LOCUS       DAJTPG010000001       136622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTPG010000001 DAJTPG010000000
VERSION     DAJTPG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            195..1628
                     /gene="cscA"
                     /locus_tag="OO230_000001"
     CDS             195..1628
                     /gene="cscA"
                     /locus_tag="OO230_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5689920.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1636..2694)
                     /locus_tag="OO230_000002"
     CDS             complement(1636..2694)
                     /locus_tag="OO230_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5689921.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2862..3080
                     /locus_tag="OO230_000003"
                     /pseudo
     CDS             2862..3080
                     /locus_tag="OO230_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3098..4426
                     /gene="dsdA"
                     /locus_tag="OO230_000004"
     CDS             3098..4426
                     /gene="dsdA"
                     /locus_tag="OO230_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5689922.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4534..6072)
                     /gene="emrY"
                     /locus_tag="OO230_000005"
     CDS             complement(4534..6072)
                     /gene="emrY"
                     /locus_tag="OO230_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5689923.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6072..7235)
                     /gene="emrK"
                     /locus_tag="OO230_000006"
     CDS             complement(6072..7235)
                     /gene="emrK"
                     /locus_tag="OO230_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5689924.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7651..8265
                     /gene="evgA"
                     /locus_tag="OO230_000007"
     CDS             7651..8265
                     /gene="evgA"
                     /locus_tag="OO230_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5689925.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8270..11863
                     /gene="evgS"
                     /locus_tag="OO230_000008"
     CDS             8270..11863
                     /gene="evgS"
                     /locus_tag="OO230_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5689926.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11919..13064)
                     /gene="yfdE"
                     /locus_tag="OO230_000009"
     CDS             complement(11919..13064)
                     /gene="yfdE"
                     /locus_tag="OO230_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5689927.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13138..14082)
                     /gene="yfdV"
                     /locus_tag="OO230_000010"
     CDS             complement(13138..14082)
                     /gene="yfdV"
                     /locus_tag="OO230_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5689928.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14152..15846)
                     /gene="oxc"
                     /locus_tag="OO230_000011"
     CDS             complement(14152..15846)
                     /gene="oxc"
                     /locus_tag="OO230_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5689929.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15900..17150)
                     /gene="frc"
                     /locus_tag="OO230_000012"
     CDS             complement(15900..17150)
                     /gene="frc"
                     /locus_tag="OO230_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5689930.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17663..18286)
                     /locus_tag="OO230_000013"
     CDS             complement(17663..18286)
                     /locus_tag="OO230_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5689931.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18583..18858
                     /locus_tag="OO230_000014"
     CDS             18583..18858
                     /locus_tag="OO230_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5689932.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18935..19177)
                     /locus_tag="OO230_000015"
     CDS             complement(18935..19177)
                     /locus_tag="OO230_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5689933.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19530..20450
                     /gene="lpxP"
                     /locus_tag="OO230_000016"
     CDS             19530..20450
                     /gene="lpxP"
                     /locus_tag="OO230_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5689934.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20805..20876
                     /gene="ypdK"
                     /locus_tag="OO230_000017"
     CDS             20805..20876
                     /gene="ypdK"
                     /locus_tag="OO230_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5689935.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20941..22179)
                     /gene="alaC"
                     /locus_tag="OO230_000018"
     CDS             complement(20941..22179)
                     /gene="alaC"
                     /locus_tag="OO230_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5689936.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22556..24042
                     /locus_tag="OO230_000019"
                     /pseudo
     CDS             22556..24042
                     /locus_tag="OO230_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24057..24791
                     /gene="ypdB"
                     /locus_tag="OO230_000020"
     CDS             24057..24791
                     /gene="ypdB"
                     /locus_tag="OO230_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5689937.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24804..25661
                     /locus_tag="OO230_000021"
                     /pseudo
     CDS             24804..25661
                     /locus_tag="OO230_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25664..28159)
                     /gene="ptsP"
                     /locus_tag="OO230_000022"
     CDS             complement(25664..28159)
                     /gene="ptsP"
                     /locus_tag="OO230_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5689938.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28184..29221)
                     /gene="ypdE"
                     /locus_tag="OO230_000023"
     CDS             complement(28184..29221)
                     /gene="ypdE"
                     /locus_tag="OO230_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5689939.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29221..30304)
                     /gene="ypdF"
                     /locus_tag="OO230_000024"
                     /pseudo
     CDS             complement(29221..30304)
                     /gene="ypdF"
                     /locus_tag="OO230_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30319..31566)
                     /locus_tag="OO230_000025"
     CDS             complement(30319..31566)
                     /locus_tag="OO230_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5689940.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31588..31914)
                     /locus_tag="OO230_000026"
     CDS             complement(31588..31914)
                     /locus_tag="OO230_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5689941.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32133..33098)
                     /gene="glk"
                     /locus_tag="OO230_000027"
     CDS             complement(32133..33098)
                     /gene="glk"
                     /locus_tag="OO230_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5689942.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33302..34558
                     /locus_tag="OO230_000028"
     CDS             33302..34558
                     /locus_tag="OO230_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5689943.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34673..34999
                     /locus_tag="OO230_000029"
     CDS             34673..34999
                     /locus_tag="OO230_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5689944.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35140..36378)
                     /locus_tag="OO230_000030"
     CDS             complement(35140..36378)
                     /locus_tag="OO230_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5689945.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36714..37916
                     /gene="nupC"
                     /locus_tag="OO230_000031"
     CDS             36714..37916
                     /gene="nupC"
                     /locus_tag="OO230_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5689946.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37966..40078)
                     /locus_tag="OO230_000032"
                     /pseudo
     CDS             complement(37966..40078)
                     /locus_tag="OO230_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40286..40361)
                     /locus_tag="OO230_000033"
     tRNA            complement(40286..40361)
                     /locus_tag="OO230_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40326..40328),aa:Ala,seq:ggc)
     gene            complement(40401..40476)
                     /locus_tag="OO230_000034"
     tRNA            complement(40401..40476)
                     /locus_tag="OO230_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40441..40443),aa:Ala,seq:ggc)
     gene            40697..41056
                     /locus_tag="OO230_000035"
     CDS             40697..41056
                     /locus_tag="OO230_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5689947.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41058..41450
                     /locus_tag="OO230_000036"
     CDS             41058..41450
                     /locus_tag="OO230_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5689948.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41502..42917)
                     /gene="gltX"
                     /locus_tag="OO230_000037"
     CDS             complement(41502..42917)
                     /gene="gltX"
                     /locus_tag="OO230_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5689949.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43176..43251
                     /locus_tag="OO230_000038"
     tRNA            43176..43251
                     /locus_tag="OO230_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43209..43211,aa:Val,seq:tac)
     gene            43296..43371
                     /locus_tag="OO230_000039"
     tRNA            43296..43371
                     /locus_tag="OO230_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43329..43331,aa:Val,seq:tac)
     gene            43376..43451
                     /locus_tag="OO230_000040"
     tRNA            43376..43451
                     /locus_tag="OO230_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43409..43411,aa:Lys,seq:ttt)
     gene            43482..43607
                     /locus_tag="OO230_000041"
     ncRNA           43482..43607
                     /ncRNA_class="other"
                     /locus_tag="OO230_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43727..44068
                     /locus_tag="OO230_000042"
     CDS             43727..44068
                     /locus_tag="OO230_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5689950.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44059..44985)
                     /locus_tag="OO230_000043"
     CDS             complement(44059..44985)
                     /locus_tag="OO230_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5689951.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45075..46073
                     /locus_tag="OO230_000044"
     CDS             45075..46073
                     /locus_tag="OO230_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5689952.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46070..46288)
                     /locus_tag="OO230_000045"
     CDS             complement(46070..46288)
                     /locus_tag="OO230_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5689953.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46290..48305)
                     /gene="ligA"
                     /locus_tag="OO230_000046"
     CDS             complement(46290..48305)
                     /gene="ligA"
                     /locus_tag="OO230_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5689954.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48376..49362)
                     /gene="zipA"
                     /locus_tag="OO230_000047"
     CDS             complement(48376..49362)
                     /gene="zipA"
                     /locus_tag="OO230_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5689955.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49592..50353
                     /gene="cysZ"
                     /locus_tag="OO230_000048"
     CDS             49592..50353
                     /gene="cysZ"
                     /locus_tag="OO230_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5689956.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50538..51509
                     /gene="cysK"
                     /locus_tag="OO230_000049"
     CDS             50538..51509
                     /gene="cysK"
                     /locus_tag="OO230_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5689957.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51893..52150
                     /gene="ptsH"
                     /locus_tag="OO230_000050"
     CDS             51893..52150
                     /gene="ptsH"
                     /locus_tag="OO230_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5689958.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52195..53922
                     /gene="ptsI"
                     /locus_tag="OO230_000051"
     CDS             52195..53922
                     /gene="ptsI"
                     /locus_tag="OO230_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5689959.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53963..54472
                     /gene="crr"
                     /locus_tag="OO230_000052"
     CDS             53963..54472
                     /gene="crr"
                     /locus_tag="OO230_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5689960.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54514..55365)
                     /gene="pdxK"
                     /locus_tag="OO230_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54514..55365)
                     /gene="pdxK"
                     /locus_tag="OO230_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5689961.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55470..55844
                     /locus_tag="OO230_000054"
     CDS             55470..55844
                     /locus_tag="OO230_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5689962.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55877..56600
                     /locus_tag="OO230_000055"
                     /pseudo
     CDS             55877..56600
                     /locus_tag="OO230_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56804..57715)
                     /gene="cysM"
                     /locus_tag="OO230_000056"
     CDS             complement(56804..57715)
                     /gene="cysM"
                     /locus_tag="OO230_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5689963.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57849..58946)
                     /gene="cysA"
                     /locus_tag="OO230_000057"
     CDS             complement(57849..58946)
                     /gene="cysA"
                     /locus_tag="OO230_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5689964.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58936..59811)
                     /gene="cysW"
                     /locus_tag="OO230_000058"
     CDS             complement(58936..59811)
                     /gene="cysW"
                     /locus_tag="OO230_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5689965.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59811..60644)
                     /gene="cysT"
                     /locus_tag="OO230_000059"
     CDS             complement(59811..60644)
                     /gene="cysT"
                     /locus_tag="OO230_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5689966.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60644..61660)
                     /gene="cysP"
                     /locus_tag="OO230_000060"
     CDS             complement(60644..61660)
                     /gene="cysP"
                     /locus_tag="OO230_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5689967.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61964..62755)
                     /gene="ucpA"
                     /locus_tag="OO230_000061"
     CDS             complement(61964..62755)
                     /gene="ucpA"
                     /locus_tag="OO230_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5689968.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62942..63742)
                     /gene="murR"
                     /locus_tag="OO230_000062"
     CDS             complement(62942..63742)
                     /gene="murR"
                     /locus_tag="OO230_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5689969.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63906..64802
                     /gene="murQ"
                     /locus_tag="OO230_000063"
     CDS             63906..64802
                     /gene="murQ"
                     /locus_tag="OO230_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5689970.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64806..66230
                     /gene="murP"
                     /locus_tag="OO230_000064"
     CDS             64806..66230
                     /gene="murP"
                     /locus_tag="OO230_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5689971.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66410..67309)
                     /gene="yfeX"
                     /locus_tag="OO230_000065"
     CDS             complement(66410..67309)
                     /gene="yfeX"
                     /locus_tag="OO230_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5689972.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67405..67980)
                     /locus_tag="OO230_000066"
     CDS             complement(67405..67980)
                     /locus_tag="OO230_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5689973.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68041..68490)
                     /locus_tag="OO230_000067"
     CDS             complement(68041..68490)
                     /locus_tag="OO230_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5689974.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68477..68902)
                     /locus_tag="OO230_000068"
     CDS             complement(68477..68902)
                     /locus_tag="OO230_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5689975.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69116..69985
                     /gene="amiA"
                     /locus_tag="OO230_000069"
     CDS             69116..69985
                     /gene="amiA"
                     /locus_tag="OO230_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5689976.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69989..70888
                     /gene="hemF"
                     /locus_tag="OO230_000070"
     CDS             69989..70888
                     /gene="hemF"
                     /locus_tag="OO230_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5689977.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70894..71946)
                     /gene="eutR"
                     /locus_tag="OO230_000071"
                     /pseudo
     CDS             complement(70894..71946)
                     /gene="eutR"
                     /locus_tag="OO230_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71992..72492)
                     /gene="eutK"
                     /locus_tag="OO230_000072"
     CDS             complement(71992..72492)
                     /gene="eutK"
                     /locus_tag="OO230_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5689978.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72505..73164)
                     /gene="eutL"
                     /locus_tag="OO230_000073"
     CDS             complement(72505..73164)
                     /gene="eutL"
                     /locus_tag="OO230_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5689979.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74346..75137
                     /locus_tag="OO230_000074"
     CDS             74346..75137
                     /locus_tag="OO230_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5689980.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75857..76252
                     /locus_tag="OO230_000075"
     CDS             75857..76252
                     /locus_tag="OO230_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5689981.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76270..76533
                     /locus_tag="OO230_000076"
     CDS             76270..76533
                     /locus_tag="OO230_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5689982.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76556..77098
                     /locus_tag="OO230_000077"
     CDS             76556..77098
                     /locus_tag="OO230_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5689983.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77237..77524
                     /locus_tag="OO230_000078"
     CDS             77237..77524
                     /locus_tag="OO230_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5689984.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77563..77769
                     /locus_tag="OO230_000079"
     CDS             77563..77769
                     /locus_tag="OO230_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5689985.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78081..78566
                     /locus_tag="OO230_000080"
     CDS             78081..78566
                     /locus_tag="OO230_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5689986.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78547..78825
                     /locus_tag="OO230_000081"
     CDS             78547..78825
                     /locus_tag="OO230_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5689987.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78818..80278
                     /locus_tag="OO230_000082"
     CDS             78818..80278
                     /locus_tag="OO230_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5689988.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80312..81121
                     /gene="murI"
                     /locus_tag="OO230_000083"
     CDS             80312..81121
                     /gene="murI"
                     /locus_tag="OO230_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5689989.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81240..81934)
                     /locus_tag="OO230_000084"
     CDS             complement(join(81240..81688,81688..81934))
                     /locus_tag="OO230_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5689990.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82453..83826)
                     /gene="eutA"
                     /locus_tag="OO230_000085"
     CDS             complement(82453..83826)
                     /gene="eutA"
                     /locus_tag="OO230_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5689991.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83801..84280)
                     /gene="eutP"
                     /locus_tag="OO230_000086"
     CDS             complement(83801..84280)
                     /gene="eutP"
                     /locus_tag="OO230_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5689992.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84293..84628)
                     /gene="eutS"
                     /locus_tag="OO230_000087"
     CDS             complement(84293..84628)
                     /gene="eutS"
                     /locus_tag="OO230_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5689993.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84921..87200)
                     /gene="maeB"
                     /locus_tag="OO230_000088"
     CDS             complement(84921..87200)
                     /gene="maeB"
                     /locus_tag="OO230_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5689994.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87489..88439
                     /gene="tal"
                     /locus_tag="OO230_000089"
     CDS             87489..88439
                     /gene="tal"
                     /locus_tag="OO230_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5689995.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88459..90462
                     /gene="tkt"
                     /locus_tag="OO230_000090"
     CDS             88459..90462
                     /gene="tkt"
                     /locus_tag="OO230_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5689996.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90565..91608)
                     /locus_tag="OO230_000091"
     CDS             complement(90565..91608)
                     /locus_tag="OO230_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5689997.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91734..92309)
                     /gene="nudK"
                     /locus_tag="OO230_000092"
     CDS             complement(91734..92309)
                     /gene="nudK"
                     /locus_tag="OO230_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5689998.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92377..94356)
                     /gene="aegA"
                     /locus_tag="OO230_000093"
     CDS             complement(92377..94356)
                     /gene="aegA"
                     /locus_tag="OO230_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5689999.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94562..96279
                     /gene="narQ"
                     /locus_tag="OO230_000094"
                     /pseudo
     CDS             94562..96279
                     /gene="narQ"
                     /locus_tag="OO230_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96481..99594
                     /gene="acrD"
                     /locus_tag="OO230_000095"
     CDS             96481..99594
                     /gene="acrD"
                     /locus_tag="OO230_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5690000.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99693..99752)
                     /gene="ypfM"
                     /locus_tag="OO230_000096"
     CDS             complement(99693..99752)
                     /gene="ypfM"
                     /locus_tag="OO230_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5690001.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100133..100489
                     /locus_tag="OO230_000097"
     CDS             100133..100489
                     /locus_tag="OO230_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5690002.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100493..101620
                     /gene="dapE"
                     /locus_tag="OO230_000098"
     CDS             100493..101620
                     /gene="dapE"
                     /locus_tag="OO230_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5690003.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101648..101848
                     /locus_tag="OO230_000099"
     CDS             101648..101848
                     /locus_tag="OO230_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5690004.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101958..102656)
                     /gene="ypfH"
                     /locus_tag="OO230_000100"
     CDS             complement(101958..102656)
                     /gene="ypfH"
                     /locus_tag="OO230_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5690005.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102730..104745)
                     /gene="tmcA"
                     /locus_tag="OO230_000101"
     CDS             complement(102730..104745)
                     /gene="tmcA"
                     /locus_tag="OO230_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5690006.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104760..105623)
                     /locus_tag="OO230_000102"
     CDS             complement(104760..105623)
                     /locus_tag="OO230_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5690007.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105791..106504)
                     /gene="purC"
                     /locus_tag="OO230_000103"
     CDS             complement(105791..106504)
                     /gene="purC"
                     /locus_tag="OO230_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5690008.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106717..107751)
                     /gene="bamC"
                     /locus_tag="OO230_000104"
     CDS             complement(106717..107751)
                     /gene="bamC"
                     /locus_tag="OO230_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5690009.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107768..108646)
                     /gene="dapA"
                     /locus_tag="OO230_000105"
     CDS             complement(107768..108646)
                     /gene="dapA"
                     /locus_tag="OO230_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5690010.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108792..109364
                     /locus_tag="OO230_000106"
     CDS             108792..109364
                     /locus_tag="OO230_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5690011.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109364..109834
                     /gene="bcp"
                     /locus_tag="OO230_000107"
     CDS             109364..109834
                     /gene="bcp"
                     /locus_tag="OO230_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5690012.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110087..110704
                     /gene="hyfA"
                     /locus_tag="OO230_000108"
     CDS             110087..110704
                     /gene="hyfA"
                     /locus_tag="OO230_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5690013.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110704..112721
                     /gene="hyfB"
                     /locus_tag="OO230_000109"
                     /pseudo
     CDS             110704..112721
                     /gene="hyfB"
                     /locus_tag="OO230_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112732..113679
                     /gene="hyfC"
                     /locus_tag="OO230_000110"
     CDS             112732..113679
                     /gene="hyfC"
                     /locus_tag="OO230_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5690014.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113696..115135
                     /gene="hyfD"
                     /locus_tag="OO230_000111"
     CDS             113696..115135
                     /gene="hyfD"
                     /locus_tag="OO230_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5690015.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115147..115797
                     /gene="hyfE"
                     /locus_tag="OO230_000112"
     CDS             115147..115797
                     /gene="hyfE"
                     /locus_tag="OO230_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5690016.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115802..117388
                     /gene="hyfF"
                     /locus_tag="OO230_000113"
     CDS             115802..117388
                     /gene="hyfF"
                     /locus_tag="OO230_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5690017.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            117378..119092
                     /gene="hyfG"
                     /locus_tag="OO230_000114"
                     /pseudo
     CDS             117378..119092
                     /gene="hyfG"
                     /locus_tag="OO230_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119102..119647
                     /gene="hyfH"
                     /locus_tag="OO230_000115"
     CDS             119102..119647
                     /gene="hyfH"
                     /locus_tag="OO230_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5690018.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119644..120402
                     /gene="hyfI"
                     /locus_tag="OO230_000116"
     CDS             119644..120402
                     /gene="hyfI"
                     /locus_tag="OO230_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5690019.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120395..120808
                     /gene="hyfJ"
                     /locus_tag="OO230_000117"
     CDS             120395..120808
                     /gene="hyfJ"
                     /locus_tag="OO230_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5690020.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120838..122667
                     /gene="hyfR"
                     /locus_tag="OO230_000118"
     CDS             120838..122667
                     /gene="hyfR"
                     /locus_tag="OO230_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5690021.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122668..122850
                     /locus_tag="OO230_000119"
     CDS             122668..122850
                     /locus_tag="OO230_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5690022.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122872..123744
                     /locus_tag="OO230_000120"
                     /pseudo
     CDS             122872..123744
                     /locus_tag="OO230_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123782..124843)
                     /locus_tag="OO230_000121"
     CDS             complement(123782..124843)
                     /locus_tag="OO230_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5690023.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            125056..126519
                     /gene="bepA"
                     /locus_tag="OO230_000122"
     CDS             125056..126519
                     /gene="bepA"
                     /locus_tag="OO230_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5690024.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            126540..126899
                     /gene="arsC"
                     /locus_tag="OO230_000123"
     CDS             126540..126899
                     /gene="arsC"
                     /locus_tag="OO230_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5690025.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(127032..127778)
                     /gene="hda"
                     /locus_tag="OO230_000124"
     CDS             complement(127032..127778)
                     /gene="hda"
                     /locus_tag="OO230_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5690026.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127828..129117)
                     /gene="uraA"
                     /locus_tag="OO230_000125"
     CDS             complement(127828..129117)
                     /gene="uraA"
                     /locus_tag="OO230_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5690027.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129203..129829)
                     /gene="upp"
                     /locus_tag="OO230_000126"
     CDS             complement(129203..129829)
                     /gene="upp"
                     /locus_tag="OO230_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5690028.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            130153..131190
                     /gene="purM"
                     /locus_tag="OO230_000127"
     CDS             130153..131190
                     /gene="purM"
                     /locus_tag="OO230_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5690029.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131190..131828
                     /gene="purN"
                     /locus_tag="OO230_000128"
     CDS             131190..131828
                     /gene="purN"
                     /locus_tag="OO230_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5690030.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            132000..134066
                     /gene="ppk1"
                     /locus_tag="OO230_000129"
     CDS             132000..134066
                     /gene="ppk1"
                     /locus_tag="OO230_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5690031.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            134071..135612
                     /gene="ppx"
                     /locus_tag="OO230_000130"
     CDS             134071..135612
                     /gene="ppx"
                     /locus_tag="OO230_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5690032.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135651..>136622)
                     /gene="pdeF"
                     /locus_tag="OO230_000131"
     CDS             complement(135651..>136622)
                     /gene="pdeF"
                     /locus_tag="OO230_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5690033.1"
                     /translation="LKQFRFFWDGMPMQPQIGVSYCYVRSPVDHIYLLLGELNTVAEL
                     SIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHE
                     ILLRMKGENDELIGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAIN
                     LSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQ
                     IAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRV
                     VAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34109 a        35150 c        33570 g        33793 t
ORIGIN      
        1 gagttacgca tagtgataaa cctctttctc gcaaaatcgt catggattta ctaaaacatg
       61 catattcgat cacaaaacgc catagttaac gttaacattt gcgatattca tcgcatttat
      121 gaaagtaagg gactatattt ttataaaagt taacgttaat aattcaccaa atttgcttaa
      181 ccaggatgat taaaatgacg caatctcgat tgcatgcggc gcaaaacgca ctagcaaaac
      241 ttcatgagca ccggggtaac actttctatc cccattttca cctcgcgcct cctgccggat
      301 ggatgaacga tccaaacggc ctgatctggt ttaacgatcg ttatcacgcg ttttatcaac
      361 atcacccgat gagcgaacac tggggaccaa tgcactgggg acatgccacc agtgacgata
      421 tgatccactg gcagcatgag cctattgcgc tagcgctagg agacgataat gacaaagacg
      481 ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct ctcacttatc tacaccggac
      541 acgtctggct cgatggtgca ggtaatgacg atgcaattcg cgaagtacaa tgtctggcta
      601 ccagtcggga tggtattcat ttcgagaaac agggtgtgat cctcactcca ccagaaggaa
      661 tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga cacatggtgg atggtagtcg
      721 gggcgaaaga tccaggcaac acggggcaga tcctgcttta tcgcggcagt tcattgcgtg
      781 aatgggcctt cgatcgcgta ctggcccacg ctgatgcggg tgaaagctat atgtgggaat
      841 gtccggactt tttcagcctt ggcgatcagc attatctgat gttttccccg cagggaatga
      901 atgccgaggg atacagttac cgaaatcgct ttcaaagtgg cgtaataccc ggaatgtggt
      961 cgccaggacg actttttgca caatccgggc attttactga acttgataac gggcatgact
     1021 tttatgcacc acaaagcttt ttagcgaagg atggtcggcg tattgttatc ggctggatgg
     1081 atatgtggga atcgccaatg ccctcaaaac gtgaaggatg ggcaggctgc atgacgctgg
     1141 cgcgcgagct atcagagagc aatggcaaac ttctacaacg cccggttcac gaagctgagt
     1201 cgttacgcca gcagcatcaa tctgtctctc cccgcagaat cagcaataaa tatgttttgc
     1261 aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc gctgaagaac agtgatgccg
     1321 aacattacgg attacagctc ggcactggaa tgcggctgta tattgataac caatctgagc
     1381 gacttatttt gtggcggtat tacccacacg agaatttaga cggctaccgt agtattcccc
     1441 tcccgcagcg tgacacgctc gccctaagga tatttatcga tacatcatcc gtggaagtat
     1501 ttattaacga cggggaagcg gtgatgagta gtcgaatcta tccgcagcca gaagaacggg
     1561 aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca acatggagca ctctggctac
     1621 tgggttaaca taatatcagg tggaacaacg gatcaacagc gggcaaggga tccgcgtcac
     1681 tcttccccct tcacgacctt caataatatg caatgcagct tcccgcccga taatgtcatg
     1741 tggaagctga attgtggtca gcggcggtaa aaacagatgc ccgacgccaa ccagattatc
     1801 aaagcccatt acagcgacat cctgcgggat acgtaccccc ttcgccaaaa gaacctgata
     1861 agccacaaag gctgcgcgat cgttaccaca tatcagaaca tcaaaatctg gtttgcccga
     1921 tttgaagtga gcattgagta aacttgcgag atcggtgtag tgatcatcac ctgttgccat
     1981 gtgaaattgt ttcacctcag ccagatctcg tccagcatca cgccaagcct gctcaaatcc
     2041 ctgccgacga taccctgttg ccaacgcact ttccggtagc cagaagcata atggttgacg
     2101 atagcccgcc gcgagcaaat gctgtgttga ttcatattgt gcagtgtaat catcagggat
     2161 ataactgggt aacgctgggt catccgccac acagttcgcc aatacaatat tttcaccata
     2221 cagagactca ggcagcgtga tatgtcgcag ccccattgta gtatagataa tgccatccgg
     2281 acggtgggca agcagctgac gtgccgcgcg ggcagcgtca tcttcagaaa aaatattgat
     2341 taaaaaacta ttccagccga actcgctggc ggtttgctca atggcaagca gaatatcaac
     2401 agagaaagga gtggtagccg tgtcctgcgc cagcacggcg agagtcgacg gcttacgtcc
     2461 ttgagcgcgc atcttacggg cggaaagatc aggaacataa ttcagggtct ggattgcctg
     2521 caatacgcgg tcacgcgttg caggacgcac agattctgca ttatgcatca cccgggagac
     2581 tgtcatcatc gacactcccg ccaggcgtgc gacatccttt aatgaagcca tacccaagcc
     2641 gtttgccgta aaacgggcac tgtagcagaa acagacgtca ctggcgagat ccaacgccct
     2701 atcacctgac acagcaatac aataaaaaat aacaataatt cccggacaat tgtccccaat
     2761 tccgcctctg ttctcgccaa cgagtaacca tataggcaag taagtaaaac atgccaactg
     2821 gcatcgccaa cctggagaag aaaatataaa tatggatcat tgtgctgcca ctgtatcccg
     2881 acatcagccc ggaaattatt gcgattgcta tcggttcagg tgcaattggc tgcacgatcg
     2941 ttacggactc gcttttctgg ctggtgaagc aatattgcgg cgctacgctc aatgaaacat
     3001 ttaaatacta tacgacagcg acatttatcg cttcagtcgt cgctctggcg ggcacattcc
     3061 tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta aaatgaactc
     3121 gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttaaagaaa ccacctggtt
     3181 taatcctagc acgacctcat tggctgaagg tttaccttat gttggcctga ccgaacagga
     3241 tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa aagcatttgc
     3301 tgaaacagct gccactgggg ggattattga atcagaactg gttgccattc cggctatgca
     3361 aaaacggctg gaaaaggaat atcaacaacc gatcagcggg caactcctac tgaaaaaaga
     3421 tagccatttg cccatttccg gctctataaa agcacgcggc gggatttatg aagtcctggc
     3481 acatgcagaa aaactggctc tggaagcggg gttgctgacg cttgatgatg actacagcaa
     3541 actgctttct ccggagttta aacagttctt tagccaatac agcattgctg tgggctcaac
     3601 cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta aggtgacagt
     3661 tcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc atggcgttac
     3721 ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta aagcagcgca
     3781 gtctgacccg aactgtttct ttattgatga cgaaaattcc cgcacgttgt tccttgggta
     3841 ttccgttgct ggccagcgtc ttaaagcgca atttgcccag caaggccgta tcgtcgatgc
     3901 tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg gtggcgtcgc
     3961 attcgggctt aaactggcgt ttggcgatca tgttcactgc ttttttgccg aaccaacgca
     4021 ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt ctgttcagga
     4081 tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg catcatgctt
     4141 tgtcgggcgg gcaatggagc gtctgctgga tggcttctat acccttagcg atcaaaccat
     4201 gtatgacatg cttggctggc tggcgcagga agaaggtatt cgtcttgaac cttcagcact
     4261 ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta agttaccaac agatgcacgg
     4321 tttcagcgca gaacaattgc gtaatgccac tcatctggtg tgggcgacgg gaggtggaat
     4381 ggtgccggaa gaagagatga atcaatatct ggcaaaaggc cgttaataac gtttcaacgc
     4441 aacatcgcaa tcctttccct gggtgagcga tgctgccgat ggcgcagact taagatcccc
     4501 ggtcttaccc gctataaccc cctcctttta tcatcaccca acgcctttcg ctgtaaacgg
     4561 cggtttcgca aaccaaacca aaaccgtaag taagataaaa gcaatagccg ccatacggaa
     4621 aatctcattt gcagaaatag aaagtgactg ctgggttatt tcattattaa tttcattaag
     4681 aactcctgaa agcgaaccat agtatttatc cataatttgt gacgatgaat taaacacggg
     4741 gttaaattga tcgatggttg ctgtcaactg actatggtgt aacgattcgc gtcgtcccca
     4801 cagcgtcatt gtcaacgacg taccaactga tcctgacaag gtacgaaaaa aattactcat
     4861 actcgaggca ttggcaaatt tattatctgg caagcctgaa aacgaaatcg ttgttaaggg
     4921 taaaaagaaa caggcaacgg cgaatccctg gaaaaactgt ggcaaaatga tgcctgtaaa
     4981 atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat acatcaaaaa
     5041 actgaatgtc actaacaccc gcatgtctat tttattgcca taacgtccta tcaaaggtga
     5101 aatcaatagt ggcatgatgc cgatgggcgc ataagcaagc ccggcccata tcgcattata
     5161 ccccatcgtt ttctggagta actgcggcat aaggacgatc gctccagagt aaaataaata
     5221 cgcgcaggtg atactcacaa taccaatggt gaaattacgg gacttaaaca aactgagatc
     5281 aagaatcggg ttctctgagg tcgattccca aatgactaaa gagatcagag aaataactga
     5341 tactactgtt aatattatta ttgtactcga gttgaaccaa tccagatcgc gccctttatc
     5401 aagcataatt tgcaagccac caacaccgag cactaacagg gtcagtcttg gtagattcat
     5461 ttttaccggt gaagtttcag tttctcttcc tttaagtaag gttaagcata atgtcaggac
     5521 gataatcccc atagggacat tgattaaaaa tatccaaccc cagctaaagt tatcgcaaat
     5581 atagccgccc aatatcggcc cacatatcgg agcgataatc acggtcattg accataatgc
     5641 cagagcaaat gttctttttt ctggtggata attccttaat aataaactct gtgacagtgg
     5701 aattaacggc cccgccatta atccctgaac gactctaaaa aatatcagca catcaagatt
     5761 ggtcgataaa ctacacatta aggaagacag cgaaaaaaaa gtgactgaaa gtaaaaataa
     5821 ccttaattcg cctattcttt gtgccagcct gccggtaaca gggatcgcaa tggcatttgc
     5881 tacgccaaac gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca gaaagccaga
     5941 tattgtcggt attgcgacgt tagaaatagt ggaatccaac atttgcataa acgtcgctaa
     6001 tgacaatgca atagtaacgc accataacgt cccaccggtt aatggtgccg gagttgattt
     6061 agtgattgcc attaaagttg tccattatgc gaaataatgt tgctaatttc tttttctatc
     6121 ggactggtat cgataactaa agccttactg gtataagcag gcatggaggt cacggttgaa
     6181 gccagatcag gcatctcagc aatgtcttcg tccttcgtat caatagttgc agtcatcgat
     6241 aaaccaatgc gcaaggggtg ttccatgagt tcttttggat caagagaaac ttcaaccggt
     6301 acacgctgaa cgattttgat ccagttccct gtcgcatttt gtgcaggtaa taaggagaac
     6361 gcattgccgg ttcccatatt gatccctgtc acccgaccat gaaacacaac attttcacca
     6421 taaagatcgc tgataatatt gactgattga ccaatccgta catccgtgag ttgtgtttct
     6481 ttaaagttgg cattaaccca catttgacgt gccggtacga cagccattaa cgattgtccg
     6541 gaactcactg tttcgccgac ctgaacactt cgctgggcaa tatagccagt aaccggactc
     6601 cttatatccg tacgtttaag caccaaccag gcttctttgg ttgcatccgc cgcttcaact
     6661 acttgtggct gacggtttaa tggtgtgttc attactaaag ctttattcgc tttataagcc
     6721 tggatagcgg cattcagtgc cgctttgcta cttattaacg tatctttggt atgctccagc
     6781 gtttcttttg aaataacccc ctgcttcgct aaaggcacac gacggttata atcttctaaa
     6841 gattgttgat actgaatacg tgctgaagcg acttcggcac tgtattgttt atcctgtaag
     6901 tacagtttat tcgtttgccg aacaatattt gccagattat ttttagcttt attgagtgcg
     6961 atagtggcat cagttttatc cagtgaaact aaaatgtcac cctgtcgaac gtagttcgta
     7021 tctttatgat taactacagt gacactacct gagacttgtg cagaaattgg atctgcattc
     7081 cccgtgacat aggcgtcatc tgtactaatc atgtcttcta attccattga ccaataggca
     7141 taggcacctg aaaacgcaat aaataaaact atcaccaata aagaaaagta tttttttctg
     7201 ttagactgtt ttttatttga attaatctgt tccactatta tctctcattt cgcatagatg
     7261 aaatttatga attgaatgaa aagagtattc attcttcata tcaaacacag tgcatgtgtt
     7321 gcacattatc ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa aaacaacaga
     7381 ggactaaacc gtggcttttg caatacaatt cttacgcctg taggattagt gagaagactt
     7441 atagtgctaa cttgaaacta taaatcatcg gtataatccc tgattttatt gttgacattt
     7501 catttatgcc gactatttat atggtatact tgtcgaatta tcttaaagga aactcagatt
     7561 ttcttatttt tattgagaaa atgagatgac gccttatgtc tgtattacta cagggagaag
     7621 ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga tgaccatcct
     7681 cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat cttagcggag
     7741 ttaactgaag gcggaagcgc cgttcagcgg gtggaaacac ttaagcctga tatcgtcatc
     7801 attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct gaggaagcgc
     7861 caatatagcg gaattattat tatcgtctcc gctaaaaatg accattttta cgggaaacat
     7921 tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag aaggcatgaa caatatcatt
     7981 gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct caaccggttt
     8041 gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca agaaattagt
     8101 gtcatgcggt atattcttga tggcaaggat aataatgaca ttgcggaaaa aatgttcatc
     8161 agcaacaaaa ctgtcagcac gtataaaagt cgcctgatgg aaaaattaga atgtaaatca
     8221 ctgatggatc tttacacatt cgcgcaacgt aacaaaatcg gctaatcaca tgaagttttt
     8281 accctatttt tttcttctct gttgtggtct ttggtcgacc ataagtttcg cagacgaaga
     8341 ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc cagtacgtct
     8401 gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg cagtacacaa
     8461 gtcccaaacg gctaccttgt tgcataccga ttcgcagcaa caggttcgtg gtattaatgc
     8521 tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc gggaatacgc
     8581 agatcatcaa aaagcaatgg acgcgcttgc agaaggtgaa gtcgatatag tgttatcaca
     8641 tttagttact tcgccgcctc ttaataatga cattgctgca accaaaccat tgataattac
     8701 ctttccggcg ctggtaacca cccttcacga ctcaatgcga ccgcttacct caccaaaacc
     8761 agtaaatatt gctcgggtag caaattaccc cccagacgag gtaattcatc aatcatttcc
     8821 aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg tctcagctgg
     8881 gcacaatgat tactttattg gtagtaacat cattaccagc agtatgattt cccgctattt
     8941 cactcactcc ttaaatgtag tgaaatatta taactcgccg cgtcaatata attttttctt
     9001 gaccagaaaa gaatctgtca ttcttaatga agtactcaat agatttgttg atgctttaac
     9061 aaatgaagtt cgctatgaag tatcacaaaa ttggcttgat acaggaaacc tggcctttct
     9121 gaacaaacca ttagaactca ctgaacatga aaaacagtgg attaagcagc atcccaattt
     9181 aaaggtgctg gaaaatcctt actcgccccc ctattctatg acggatgaaa atggctcggt
     9241 tcggggcgtt atgggggaca ttcttaatat tattaccttg caaacaggtt taaatttttc
     9301 tccgatcacc gtttcacaca atatccatgc tggaacacaa cttagccccg gaggatggga
     9361 tataatacct ggcgctattt atagtgaaga tcgagaaaat aatgttttat ttgctgaagc
     9421 cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg aacaaacatt
     9481 aaaaaaagga atgaaagttg ccattccata ttattatgag ctgcattcgc aattaaaaga
     9541 gatgtatccg gaggttgaat ggatacaggt cgataatgcc agcgctgcat ttcacaagat
     9601 taaggaaggt gaacttgatg ctctggtcgc gacacagcta aattcgcgtt acatgatcga
     9661 tcattactat cctaatgaac tttatcattt tcttattcct ggcgttccga atgcatcgct
     9721 ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata aagcactgaa
     9781 tgcaattccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa tgcccaatgt
     9841 gaccattgac acatgggacc tatatagcga gcaattttat attgttacga cattatccgt
     9901 tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag ttcgtcgtcg
     9961 taaagtcatt cagggtgatt tagaaaacca aatatcattc cgaaaagcac tctcggattc
    10021 cttaccgaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc ataatagtgc
    10081 ttttgaacat tatttcactg cggattacta caaaaatgca atgttaccat tagaaaacag
    10141 tgattcaccc tttaaagatg ttttttctaa tgcgcatgaa gtcacagcag aaacgaaaga
    10201 aaatcgaaca atatacacac aggtatttga aattgataat ggcatcgaga aaagatgcat
    10261 taatcactgg catacattat gcaatcttcc tgcaagtgac aatgcagtat atatttgtgg
    10321 ttggcaagat attactgaaa cgcgtgatct aattaatgca ctcgaggtag aaaaaaataa
    10381 agcgataaag gctaccgtag caaaaagtca gtttctggca acgatgagtc acgaaataag
    10441 aacaccaata agctctatta tgggcttcct ggaacttctg tcgggttctg gtcttagcaa
    10501 ggagcaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac tcctcggctt
    10561 aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac ttcaaccaca
    10621 atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcggtg cgattgctgc
    10681 aagcaaatcg atcgcattaa gttgcagcag tacatttcct gatcattacc tggttaagat
    10741 cgaccctcag gcgtttaagc aggtcttatc aaatttactg agtaatgctc tcaaatttac
    10801 caccgagggg gcagtaaaaa ttacgacctc cctgggtcac attgatgaca accacgctgt
    10861 tatcaaaatg acgattatgg attctggaag tggattatcg caggaagaac aacaacaact
    10921 gtttaagcgc tacagccaaa caagtgcagg tcgtcagcaa acaggttctg gtttaggctt
    10981 aatgatctgc aaagaattaa ttaaaaatat gcagggcgat ttatcattag aaagtcatcc
    11041 aggcatagga acaacattta cgatcacaat cccggtagaa attagccagc aagtggcgac
    11101 tgtcgaggca aaagcagaac aacccatcac actacctgaa aagttgagca tattaatcgc
    11161 ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat taggatatga
    11221 tgttgatgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc aacattatga
    11281 tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga ctcgcaaact
    11341 ccgtgagcaa aattcttcct tacccatctg ggggcttaca gccaacgcac aggctaacga
    11401 acgtgaaaaa gggttaagtt gcggcatgaa cttatgtttg ttcaaaccgt tgaccctgga
    11461 tgtactgaaa acacatttaa gtcagttaca ccaggttgcg catattgcac ctcagtatcg
    11521 ccaccttgat atcgaagccc tgaaaaataa tacggcgaat gatctacaac tgatgcagga
    11581 gattctcatg actttccagc atgaaacgca taaagatcta cccgctgcgt ttcatgcact
    11641 agaagctggc gataacagaa ctttccatca gtgtattcat cgcatccacg gtgcggctaa
    11701 catcctgaat ttgcaaaagt tgattaatat tagccatcag ttagaaataa cacctgcttc
    11761 agatgacagt aagcctgaaa ttcttcagct gctgaactct gttaaagaac acattgcaga
    11821 gctggaccag gagattgctg ttttctgtca gaaaaatgac taaattgcgg ctcccacaat
    11881 gttctaatgt gggagctatt taccagcaca tcttacattt atgatgagaa ctcctggcga
    11941 atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg gtccgcgcag
    12001 ccgctgattt ttatcggatt acccggcatc attattcctc cggcttcaat caacatattt
    12061 ctcgcctgag tttgtggcaa attaatggcc tcagccacac ttaataacgg cgcgacgggc
    12121 acaccaactt catgtatttt ggctaaccaa acttcagctg cctgcgtttt taacgttcgc
    12181 tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct gctaaatcgg
    12241 ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa aagcttgtca
    12301 ttaccacaac aaatcgtaat cggcttatcc tgagtatcga aaacatcaaa aggtgccatg
    12361 tagggatggc gatttcccag gcgttgtggt gacttccctg tcgcaatata tgccatcaga
    12421 ccatgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg cgcccctctc
    12481 tggctctttt cgcggccata aagtgcactc actattccgc tgaataaata gacaccgccg
    12541 catagatccg caagagaggt accaacacgc actggcggag catcagggta ccctgtttcc
    12601 atcattatcc cactcattgc ctgaatgatg gtatcgtagg caggagcatc ttttagcgga
    12661 ccggtatgtc cgaaacctga cgatgaagca tatatgaggc gcgggttgat ttcttgtaac
    12721 ctttcccatg aaaaccccag tttttccatt gtacctaggc gaaaattctc agctaataca
    12781 tcagcttgtt tgagcatatt tataaatata cttttatcgt gatcattctt taaatcaaga
    12841 accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc atccacatag
    12901 ggaccaaatg tgcgggtatc atcaccatga cctggtggct caactttaat tacccttgcg
    12961 cccatattat aaagaagttg agttccgaaa ggtccattaa ggacatgtgt catatcgata
    13021 actaataagc cttcaaacgg ccctttgctt tcattatttg tcatttgctt atgccttgaa
    13081 aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca tgattaatta
    13141 tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaagc ccagtacgct
    13201 cacagccaat gacgctgtac cagtacgggt gtagacatta aaccggctgg caataatgat
    13261 tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcaaat gttcgctgtt
    13321 caaatgacat gccataccaa cgagaagcag cgccagtggc atcagaatca acttcaggaa
    13381 ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag tcaaccccgc
    13441 agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg ggtcccatgc
    13501 tgccggaatt tttaccccaa ccagcaccag gatcgttgcc agaacaggtg cccatactac
    13561 tggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac catccgctcc
    13621 tgaggaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa caataataga
    13681 aataattgct accactaaac ctgttgatac ggaatcacca taaataggat cgagaactgc
    13741 aaaccccagg aatccaatgg taggtgaacc cgcaattaat gcacatacag ctgcttctgc
    13801 atgggtacgt ttaaaaaatt tgtagcaacc aaaccaggag aaaaagaaac atccgacaat
    13861 aaccacaagt gatacaaggg tcagacgagt gtccgcaaaa atcatttccc tgtttgcccg
    13921 ggtaatagaa acaaatagag ccgcaggaag agcgtagttt aataccagtt tattaaatgc
    13981 gcgggcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca acagcatgat
    14041 tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat ttccgataag
    14101 ggtcccctca ccatcgcata atgagttggc aagaggagac gatgtcagta tttaattacc
    14161 agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg ctgcagggtc
    14221 gataaccaca ttaataatgg tcggtttgcg cgactgaata ccggtggtta aagcatgacg
    14281 aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca ttaatttgtc
    14341 ataccttgca tggtgcaaca gatccgttgg tgatggtgca ccagcgccac tgagatcaac
    14401 accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg gcaggttata
    14461 tcgacaaatc gtttcaattt ccatcccact gaaaccaaaa gcactatcac cttcaatggc
    14521 gacaaccgga gaaccagaag tcacgctagc accgatggca tagcccatac cgatgcccat
    14581 gacaccccag gtaccacaat ccagacgacg acgtggttta tacatatcaa taatatttcg
    14641 tgcattatcc agggtatttg caccttcatt aactaaataa atatcctggt tctcgcgcaa
    14701 tacgtcgcgc acagcactta atgcattaaa gtaatttaat ggttgagtat ctgtacttaa
    14761 tttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat cgcgccatac
    14821 cagtggagtc gtaaatgtgt tttgtttcag ttctgccagc ataccttgca tactggatgc
    14881 aatatcacca acgactggca cagcaatggg gcggttgctg tcaatttcct gcggttcaat
    14941 atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg ccaataacca
    15001 attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa acgaacgcgc
    15061 agctgccgca gaaagtggat gcgtatcttc aaggatccct ttcgccatag acattggcag
    15121 gaatggaatc tgagcacttt caataaattc acgaagctgt tcatcagctt gtgaatacgc
    15181 cgcgcctttg ccaaggataa ttaatggccg ttcagctttt gctaaaagcg aaattgcgct
    15241 agtgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt taacaatcgt
    15301 ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca aatcaagata
    15361 aactccgcca gggcgacccg atacagagac ccggatagcg cgtgccaatg caatgccaag
    15421 atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg cattcatttg
    15481 gtccagctct tcataatcac cttgctgtag gtcgacgatc gcgcggtcgc tggagccgct
    15541 aatcatgata atcggaaaac catttaccgt tgcgttggcc aatgcggtca aaccattgag
    15601 gaatcctggc gcagaaactg tcaggcagat ccccggtttt tgggtaagaa aaccgcttgc
    15661 cgcagcggca tagcctgccg actgctcatg acgaaaacca atataacgaa tgccttccgc
    15721 ctgggcatgg cgtgccatat ccgtcacagg aatacctaca acaccataaa tagtgtcaat
    15781 attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt gaagttgatc
    15841 tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc ccgttaatat
    15901 cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc cagctcctgc
    15961 aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg cgtaaaggca
    16021 gagaatttca ttggacaacc aacggtcaga tattttccac gcaacggttg ttccacttca
    16081 acaacactgc cactttggcg caaagaggga tcaagtgaaa tttctttcat acttaaaacc
    16141 ggtgcacaag gaatatcaaa ctgagtcaaa taggccaccg cttcatgttt atcaatagtg
    16201 acagtgtatt tttcgatttc agcaaaaata tcgaaaatat gtggctgtcg ggcatgggct
    16261 gtactgtatg ccggatcggt aatccattct ggtttgccga tggctttaca ggtgttttcc
    16321 cagttttgct cctgaatagt gaaataaata taggcgttag gatcggtttc ccagccttta
    16381 catttcagga tccagccagg ctgaccgcca ccacctgcat taccaccgcg gggaactgca
    16441 tcaccaaatg taccattcgg atactgcggg tattcttcca gataacccaa tttatcgaga
    16501 cgctgctggt cacgtaattt cacgcggcaa aggttcaata cggcatcctg cattgacatg
    16561 gtgactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag taaaccgatc
    16621 agcaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag cggcggacca
    16681 tcccaaaaac ccgtagtgga tgccgcgcca cccgctgcct gagcaacgtt ttcataggct
    16741 tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa aatcagacgt
    16801 ggattgattt cttgaatatg ctcccaggtg aagcccatgt gatcaatggc ccctggatga
    16861 aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc tttgccttcc
    16921 gctgttttgg tatttaactc aatagaacgt ttgttactgt taagcatggt gaagtaaagc
    16981 gcatcgatat caggaatatc tcgcagttgg tgacgcgtta cgtcaccaac gccgggacgt
    17041 tcaattttaa taacgtcagc gccaaaccag gccagcattt gagtacaaga tgggccagat
    17101 tgcacaccgg tgaaatcgag aactttaatt ccttgaagtg gagttgacat agcaatacct
    17161 attagttaat taaatatgtg ttaatttagc gtttatttaa ggcataacgc cctctataaa
    17221 taatctgcaa aatatcttcg tagcggcact cgatattttt aaattcctgt atcaagtact
    17281 gaaaattatt ttatcgccac ttccaattcc tttctgcatg gtaagtagtg aggacgaaaa
    17341 gaagaatatg ataaatatta attcgtggta atcagagaga gtcgcgacat ttgtgatgtt
    17401 attcttacaa ctgtaagaag cgccggtgat ttacaagtaa gagtatgatt acgcttttta
    17461 aagcgtacca taaagcataa cataataaat gatttgtgat gacacgggat tattgcggtt
    17521 tactttgagg aaaataaaat gcgtgtttat agaaccgcga gcgccaaata attttatgac
    17581 cacaacctgt cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta aatcctttac
    17641 ctcaatatac tgcctgtcag cattagttca caaacagtgc ttcgctatcg acgatgattc
    17701 catcttccgc acctttaagc gccaggtttg cctcgtagta ttgcttttta tcaagcagtt
    17761 tctgcgcatc agcaagtgca ttacgagtct gtttaagcgg catcagatat tggttttcca
    17821 ttacgccaac accggccagg cgaagctctt ccattgcgcc ctttttatcc cctttcgcca
    17881 ttttttcatt ggcaattttt atcgcggctt ctttttcggg tgttgctaca taactctcag
    17941 aaattccgac agatgcatta atgacaatat attggtcatc attgagattg gtctttttac
    18001 ctggcttagc gaactttgcc cattcggtac tgtcatctga cagcaaagcg gaagcttcat
    18061 tggtcagttc ttttgccttt tccggatcgc catgaaacag agccaggcgc gctacctgca
    18121 cgtcacgcat cgcatataaa ccttgttcag agatacgctc tgctgctgcc gttttctggg
    18181 tttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat acagttgaag
    18241 atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt acctcattct
    18301 ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag aaatcagaga
    18361 gcaacggtca ttttgaaagg gttatcttac aattgtagtt attatcgcaa agcggcattt
    18421 agtcttacag ttgtaggaat atgcctcctg tcgcagagaa ctattcttat ataccacaaa
    18481 gagatgtcat ttattatatt gatgtaaata ttgattaatt atatgtaaat acagccataa
    18541 catcgtatgg ttctcagtga accattttca ggataacttt ctatgaaagt aaacttaata
    18601 cttttcagct tatttttatt ggtctctatt atggcatgca atgtttttgc attttccatt
    18661 tctggtggtg taagtgagag gagctataaa gagactgaaa aaacatcagc gatgacgacc
    18721 acacactcta caaaacttca gccatcacag gcgattttgt ttaagatgag agaagatgtg
    18781 ccaccattaa acctcacaga agaaatcacg cctacttatc cgacaaaggc gaattatctt
    18841 attcatcctg tgcgatagcg tctgtcacat ggcaggtgaa caatcatgtc tgatgctgat
    18901 gtgaacacct gcccaaatga gccatcctga cccactatcg cctgccatac cccaccataa
    18961 agcgaatcat caagccacaa gccggggcaa gaaacataaa tatccccgtc accagttcat
    19021 taccgccagt taaccacatg gtgaagtaat aaatgagtgc agcgaggatc accatgccga
    19081 agaaagaaga gaccgccgat accacgttca gtacctgacc gatgtagccg cttacgcaga
    19141 caatacacag ggcgagaaac atccataaat aaatcatagc tcaacctcct gccgcgctaa
    19201 aaaacctgta acttcagtca ctttaatagt aagacaatcc ttaacctctg gcaatttttg
    19261 ctaagcatca atgaaaacag cacgttaaat gcaacgctat tgcgtgcgga aaatttttta
    19321 ctttgcacga ttaataatca gtagctgaaa gcagtcagcc agtgataaag tagagccacc
    19381 gcattcatgc tcctcgtggt tatgtcctga ctagtcttta cactctttac aggaaccatt
    19441 gtcgtacatg atggcccaac caattgagtg taccgctgtc gacatggaat cttctgcgat
    19501 acaacaattc gtatctacag aaggtaacta tgtttccaca atgcaaattt tcccgcgagt
    19561 ttctacatcc tcgctactgg ctcacatggt ttgggcttgg tgtactctgg ctttgggtac
    19621 agcttcctta tcctgttctc tgctttctcg gcacgcgtat tggcgcaatg gcgcgaccat
    19681 tcctgaaacg tcgtgaatct atcgcccgta aaaacctgga actttgtttc ccgcagcatt
    19741 ctgcggaaga acgcgagaag atgattgccg aaaactttcg ttcactcggc atggcgctgg
    19801 tagaaaccgg catggcatgg ttctggcccg acagtcgcgt acgtaaatgg tttgatgttg
    19861 aagggttgga taaccttaaa cgcgcacaaa tgcaaaatcg cggcgtaatg gttgtcggcg
    19921 tccattttat gtcgctggaa ctgggcggcc gcgtgatggg actgtgccaa ccaatgatgg
    19981 ctacctatcg tccacataat aatcagctga tggaatgggt gcagacccgt gggcgcatgc
    20041 gctctaacaa agcgatgatc ggcagaaata atctgcgcgg cattgtcggt gcactgaaga
    20101 aaggtgaagc ggtatggttt gctcccgatc aggattatgg tcgtaaaggc agctccttcg
    20161 cgccgttctt tgcggtggaa aatgtcgcca caaccaatgg cacctatgtt ctctcccgtc
    20221 tctctggcgc agccatgttg accgtaacga tggtaagaaa agcggattac agcggatatc
    20281 gtttgttcat caccccagag atggaaggct acccgacaga tgaaaatcaa gccgctgctt
    20341 atatgaacaa gattatcgag aaagagatca tgcgcgcacc ggagcagtac ctctggatcc
    20401 accgtcgctt taaaacgcgc ccggtgggag aatcgtcgtt gtacatttaa caaactgctc
    20461 ttactctttc cctcctcaaa tcggccataa taacctcatg atttttatag cttttttctt
    20521 aagttttttc cttcgctgaa tatctaatta ttaccgtcag aaattaaact gtcgccgata
    20581 tgagacactt gtaagttact aaaagtaaat aagaaaatgc ctcttgtctc gtttcagttt
    20641 taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga attctggggt gatgcccacg
    20701 gtaaaataca gggcttttaa ggtcgctttg cccggtgtca actcactgta tcaggtgtaa
    20761 tgaagtcatt caggcgtaac agtaattacg cggagagatg taaagtgaaa tatttcttta
    20821 tgggcatttc ttttatggtc atcgtttggg ccggtacttt tgccctgatg atctaaaagc
    20881 aaactgtgca atgaaaaaca ggagccatca ggctcctgtt tgcatttctg acgacggtgt
    20941 ttattccgcg ttttcgtgaa tatgtttgct gctgacgggt agtaaaccgt cggcacggaa
    21001 catcgcttta atccctctga tggcctgacg aatacggtcg tggttttcaa tcagtgcaaa
    21061 gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc ggcgagacac aaaccttcgc
    21121 ttcgttaagg agcttcttgg caaattccag cgatcccatg gccgcatatg gttccgggat
    21181 tttcgcccag acatacatcg aagccttcgg catttcgacc atccagcccg cttcatgcag
    21241 ccctttaacc aatacatcac gacggcgttt gtactgttcg gcaatgtcgc gcacgcactg
    21301 ttgatcgccc tcgagtgccg caatcgccgc cacctgcaac ggcgtaaaag tgccgtaatc
    21361 gtgatagctt ttgattcgcg ccagggcact gaccagcgtt ttgttgccga ccataaagcc
    21421 gatacgccag cccgccatgt tgtagctttt cgacagcgta aagaactcga ccgccacatc
    21481 gcgtgcaccc ggcacctgca taattgacgg cgctttccag ccatcgtaga cgatatcggc
    21541 ataggccagg tcatggacca ccagcacatc gtagcatttc gccagcgcca caaccttttc
    21601 gaagaactcc agttccacgc attgcgcggt tgggttcgac gggaagccga ggatcatcat
    21661 cttcggtttc ggataacttt cacgaatggc gcgctccagt tcgttgaaga aatcgacacc
    21721 ttccaccagc ggcactgagc gtacctgcgc cccggcaatc accgcgccat aaatatggat
    21781 cgggtaactt ggattcggca ccagcaccgt gtcaccatga tccagcgtcg ccagcatcag
    21841 atgcgccagg ccctctttcg aaccaatagt gacgatggct tctgattccg ggtcgatttc
    21901 aacgtcgtag cgatcctgat accagcggga aatggcgcga cgtaaccgcg gaatgccgcg
    21961 tgaagtggag taaccatgcg tgtccgggcg ctgggccaca gtacataatt tttcgacgat
    22021 atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa tcgataatat cttcgccgcg
    22081 ccgacgcgca gccattttca gttcagcggt gatgttaaaa acgtagggcg ggagacgatc
    22141 aatgcgcgta aagcgacgtt cagggcgagt gtcagccata atttcctcag attaacgtta
    22201 gcgcccggac cgtccgagcg acgctgccac aatggtggct gttttgaaaa tagcctgatt
    22261 aatttctgtc tgtccagcgt tttagaacat ttttatcaat ggatttaaat gaggaactcc
    22321 acttcaaaaa caggaaattt ttctacgcta acaagaaaat caccaccaat tttattaaca
    22381 ttataatttc aataaattta ccagccagaa gtgcattatc aagagataca ttgctaatac
    22441 accatttatc ttttaatccc cttttaaatt tatggttttt cctcaaaaac ggcctgctgg
    22501 tcatcagcct tcaggttacc tatcatagag gtttaatcct tattcagagt cacccgtgca
    22561 cgaaatattc aacatgctgc tggcggtctt cgatcgggca gcgttaatgc ttatctgcct
    22621 gttctttctc atccgtatcc gcctgtttcg cgaactgttg cacaagtcgg cgcactcccc
    22681 aaaagaattg ctcgccgtta ccgccatttt ctcgctgttc gccctgttca gcacctggtc
    22741 cggcgttccc gtagaaggct cgctggtgaa cgtacgtatt atcgcggtga tgtccggcgg
    22801 gattctgttt ggcccgtggg taggcatcat taccggcgtg attgcgggta ttcaccggta
    22861 tttaattgat atcggcggcg tgacggcgat cccctgcttt atcaccagca ttctggcggg
    22921 ttgtatatca ggctggataa acctgaaaat ccccaaagca cagcgctggc gctggatatc
    22981 gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg caaggtctgc
    23041 gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac cgatcatgtg
    23101 ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt cattagcccg
    23161 actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga tgaagcccac
    23221 cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg ggtcgtgacc
    23281 ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt acaggaaatg
    23341 gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc cgagcagcta
    23401 cgtgaaatgg caaataaggc agagattcgc gccctgcaaa gcaaaattaa tccccatttt
    23461 ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga taccgctcgc
    23521 cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa agacgatgag
    23581 caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat tgagcaggcc
    23641 cgctttggcg acaagctgac ggttatctat gatattgatg aagaggtgaa ttgctgcatt
    23701 cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat tcagccttgc
    23761 aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt acgcattgcg
    23821 gtgcgagata ccgggcacgg catcgttcca aaggtgattg agcgggtcga agcgaatgaa
    23881 atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt attgtatggc
    23941 gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta cattcctaac
    24001 caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt gatattgtga
    24061 aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg ctaattaaag
    24121 agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg ttgaagtttt
    24181 tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg ctggatggcg
    24241 tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg ttcatcaccg
    24301 cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac attctcaaac
    24361 cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc tggcaacaac
    24421 agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc attaatctgg
    24481 tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc gaagcgcacg
    24541 agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg aacattaccg
    24601 aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca ttttgtgtca
    24661 atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt ctgcgactga
    24721 aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt cgccagttaa
    24781 tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat caacaatttt
    24841 tcgccgatct gttcagcggc ctagtgctta acccgcaact actggggcgc gtctggtttg
    24901 ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc cgtctggata
    24961 tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga atggtggaag
    25021 gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac aaaccggtga
    25081 tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac gatagccgca
    25141 ccacgtcgtt gttgcatcct gctcgccaga cccagcttcc cagcctgcaa cgcggtgaag
    25201 gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag cagaatatca
    25261 ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat atgccgccgg
    25321 gtaattcaca accgcgcggc gattttctct atcacagcat ttgtaactgg gtttaggata
    25381 attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc acgcccaatc
    25441 atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat gtgcgttggg
    25501 tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt catgaagtgg
    25561 cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg cgtcagttta
    25621 gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag gttaattctg
    25681 tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac tggcggaagt
    25741 ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc atcccttcgt
    25801 tagcacccag cgtcagcatg atcaccagtt cgacttcgcc catttctgac tgccagtcaa
    25861 ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga gatttggtgt
    25921 gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc tgccagacat
    25981 cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg ccgcacagga
    26041 actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc tccagcgcca
    26101 gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca atttcctgcg
    26161 cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta tccagttgac
    26221 gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca tccaggccca
    26281 gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa atgcctaccc
    26341 atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg aacgatggcg
    26401 taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca tacagatact
    26461 gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag tggtcgatga
    26521 tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct gcatgacgca
    26581 ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc cataagatct
    26641 gatcgaggct gtgaaccatc gggatcatca actgggcgtt gccgaaactg gcggcgcgca
    26701 aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata cgtaccgcgc
    26761 gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg cttttatcgc
    26821 cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt agcacctgct
    26881 ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata tagagcattt
    26941 cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc gcttccagag
    27001 cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag gccagctgcg
    27061 cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg acctgataat
    27121 aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg tcaagcactg
    27181 caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt accgggatcg
    27241 ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc agaatcatac
    27301 ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct tcagccacca
    27361 gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc caggtgatat
    27421 gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga taatcgctgg
    27481 cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc gcccccagtc
    27541 cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca tcctgaatca
    27601 gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc aattgctgat
    27661 tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct tgcgcactgg
    27721 cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc gtacccacgc
    27781 cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg cttaatgaac
    27841 gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg ctgtcgataa
    27901 agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg ctgccgctaa
    27961 tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc agcgagcttt
    28021 tagcatcagc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt tgccactggc
    28081 tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc aaaggacaga
    28141 gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg aaatccgtca
    28201 gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt tgctccatct
    28261 gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg gtggcaggcc
    28321 ccattaccaa tgtgggtacg ccagtaccgg ttaagtgcac cgctccaccg tccgtgccgc
    28381 cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgccacg gtttcaatcc
    28441 acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc atcggcccgt
    28501 taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag caggcggtat
    28561 caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg ccgcgtaatc
    28621 ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg gcgtcgtgca
    28681 gctcacgtag taacgtcacc agcagatagc aactgaggcg atcatcaaag gctttgccca
    28741 tcacccgctg gtgagggagg acctgaaaag tggtatcgaa ggtgacgcga tcgcctggac
    28801 gaattcccgc ctgcatcact tcgtcatagg tgcgcgcgcc aatgtccacg cgcatggcgc
    28861 taacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac tcttcacggg
    28921 tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca accggcagca
    28981 cgtcaatagc gccttcgcgg gagatgctgc gcaccataaa tcccacttcg tccatatggg
    29041 cgcagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc gatcccagac
    29101 catcaaatcg cacttctttt tgcaggcgag ccgcttcttc cagcaggatc tgccgcactt
    29161 cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc gataaatcca
    29221 ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt ctgcgccttg
    29281 cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctagca aataaatccc
    29341 cggctccacg gtcagcaaca tgcctggctg tagcgtcgtg gtgtcccgcg gtgaaaaacg
    29401 cggatcttca tggacttcaa tgccgatagc gtgaccagtg ttatgaccga aatagtcgcc
    29461 aaatccagcc tcggtaatga cccggcgcgc ggcgtcgtca atctgctggc agcgcacgcc
    29521 ggggcgaatt gcagagattg ccgcgagctg tgcctgcagg acaatctgat agacgtcaaa
    29581 cagcgggtga gattcggcgc tcaccccttc gccattcacc agcaaggtgc gcgtcatatc
    29641 agcagtagcc ctgatacagc gcaccaaaat cgagagtgac aaactcgccc gctgcaacaa
    29701 tcttgtcgct ggctttgccg tgcggcagcg ccccgcgcca gccgctggcg acaatggtgt
    29761 caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca gcggctatct
    29821 cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg cgatcggcaa
    29881 tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc cgcagcacat
    29941 ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc gcggtttccc
    30001 aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca atgatttgat
    30061 tgacgatagt ggtaagcgtg ttcgttgcgt caagcaaatg cagctggtag ccttgcgcac
    30121 gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt tcacggctaa
    30181 taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc tgccgtgagg
    30241 agagaagcac cgcatccagt tgttgcacct taagccagtt gcgcagcgaa gcgagtaatg
    30301 tcatctggat tttccttctt acaggctatc gattaacaat ttgcctttgc ggaacatcat
    30361 cagacgcagg aacaccacca tcagcgcagt aatgattgcc cccagcgcga tccccgccat
    30421 ataaacgccc aagttagtga ccagcggcca tgcccagata gcggattccg ggaaccattg
    30481 cactgcgccc agccagacag ctgcggttga gccgacaatc gcgccgacca tatatgacgg
    30541 aatagcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa tccccataaa
    30601 ggccaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca ggcgtttgcc
    30661 ggttaaacga cggtcaataa tggtcgccag acccagacca atcggcggaa taacgatagc
    30721 gatagaacga gcggtgaccg gtaatacgtg gtcagtggta aagctgaagg caacgaaacc
    30781 tgcggctttg ttgatcgggc caccaaggtc gattgctgtc gccgcggcga tccccattgc
    30841 gtacatcagc gcgcccttct cacctgcggc agtcagcaca gtacggatac cgccgttgat
    30901 ccagccacca aagggagtga tgacgtagta catcgccagc atgacaaaaa tggcggaaag
    30961 aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact ggattttctg
    31021 gttcatccat ttcacaaggt aaccggcaac gatagaaata atcagcgcac caatgaaggt
    31081 ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt tcagcagttg
    31141 cgtcggctgg gtagacatca acccgccgat aaaaccagcc gggaacgcca gtttgccacc
    31201 gatagagttc gccacaaatg cagcaaacat cggaatggca aaaccaaaca acacgccgcc
    31261 gaaggactgc gacagccagg caaatttaag taaggagagg tcgaaaccgg agaatttccc
    31321 gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc tataagcaat
    31381 cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca tgcgtgaaat
    31441 ccctgacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg cctgcggatt
    31501 cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc gttttttaat
    31561 ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca tctcttcgat
    31621 ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt cataaacgtc
    31681 gcgtgattcg aaacgttcgt tatcttccgg ggtaactgcc acggaatgga tgatgatggt
    31741 cgcttcggcg atatcctgcg ccgtcaggcg attctggatg ccgtccgcgc cctgggtttc
    31801 aattttcact tcataaccgg cttctaccgc cgcttcttcc agcacctgag cggccataaa
    31861 agtgtgagcc aggcccattg ggcaggcaca taaggcaatc agtttcttac tcatcgttaa
    31921 ttcctcgttc agtgatagtt gactagtgaa attatgaacg tgcaaaacaa atcgccgtta
    31981 ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg tgacgcaggt
    32041 cggccttgtg aggggacaca aagaaaaatt gaattaactg tatgatttaa aagattatcg
    32101 ggagagttac ctcccgatat aacaggaagg atttacagaa tgtgacctaa ggtctggcgt
    32161 aaatgtgcac cggaaccgag aagacccgga tagtcatgga cgatgagata caccggaata
    32221 tcatggacat attctttaaa gcgcccttta tcttcaaatg cggcacggaa accggaggct
    32281 ttgaagaact caaggaagcg cggcacgata ccgcccgcaa taaacacgcc gccaaatgtc
    32341 ccgagattga gcgccagatt gccgccaaaa cggcccataa tgacgcaaaa cagcgacaat
    32401 gcgcggcggc aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc ttttggcttg
    32461 agattttctg gcaggcggtt gtcagctttc acaattgcgc gatacaaatt caccagccca
    32521 gggccagaaa gcacgcgctc cgccgaaaca tgaccaattt ccgcacgcaa tatttcgagg
    32581 ataatggcct cttcttcact attcggcgca aaatcaacgt gaccgccttc gcccggcaag
    32641 cttacccaac gcttatcgac atggaccaga tgcgcaaccc caagccccgt tccggcaccg
    32701 taaaccgcaa taggcttacc ttcgaccggt tctgcgccac caaactgaat cagatgctct
    32761 tttttcagca tcgggatcgc catcgataca gcggtaaaat cgttaataat ttccagatgg
    32821 ctaaaaccga gattcttttt catttcggca attgagaacg cccaggtatg gttggtcatt
    32881 gccacccagt caccggtaat tgggcaagcg atggcaatac agccgtcttt cacctcgacc
    32941 ttatgttctt caagataaac gcgaatgacc gcttcgaggc tggggtaatc aagccctgaa
    33001 taggtcttag cctgcgagat ttcaccactg gcaatatcac acagagcaag acgtgcgttg
    33061 gtgccgccca catcaccgac taatgcatac tttgtcattc ttcaactgct ccgctaaagt
    33121 caaaataatt ctttctcaca ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc
    33181 aggctccctg taaatatcga tctgggtcac acaattactt tatcgtttca gcaccaattg
    33241 cagcgatgcc tttttgcaag ctgggcaaac taagtatctg accccgcata aggaatagaa
    33301 catgctccat ccgcgagcca gaaccatgtt gttattatcg ctccccgccg tggcaattgg
    33361 gattgcgtcc agtcttattc tgattgtggt gatgaaaatc gcctcggtat tacagaattt
    33421 gctctggcaa cgactgccgg gaactctggg gatagcccag gattcacccc tctggatcat
    33481 cggtgtatta acgctaacgg gtattgcggt ggggttggtt atccgtttca gccagggtca
    33541 tgccggacca gaccccgcct gtgaaccgct gatcggcgca ccggttccgc cctctgcgct
    33601 acctagactt atcgtagcat taattctcgg tcttgctggc ggcgtcagcc tggggccgga
    33661 acatccgatc atgaccgtca atatcgccct tgccgtcgcc attggcgctc gtctgttacc
    33721 gcgcgtcaac cgaatggagt ggactatttt agcctctgcc ggaaccatcg gcgcgctgtt
    33781 tggtacacct gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggca gtagtgaagt
    33841 tccgctatgg gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg cacttactac
    33901 cggattattt ttccatcccc atttttcact gcccattgcg cattacggac agatggaaat
    33961 gaccgatatt ctcagcggtg caattgtcgc ggcgattgcc atcgcagcag ggatggtcgc
    34021 cgtatggtgc ttaccacggt tgcacgcgat gatgaatcaa atgaaaaatc cggtgctcgt
    34081 gctgggtatt ggcggattta ttctcggaat tctgggggtt attggtggac cagtttcgct
    34141 gtttaaaggg ctggatgaga tgcagcagat ggtggcaaat caggctttca gcaccagcga
    34201 ttactttttg ctggcggtaa ttaaacttgc cgccctggtc gttgctgccg ccagtggctt
    34261 tcgcggtggg cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt tgatgctgca
    34321 tgagcacgtt cccgccgttc cagcggcaat aaccgtttcc tgcgctattc tcggcatcgt
    34381 gctggtggta acacgcgatg gctggttaag tctttttatg gcggcagtcg ttgtacccaa
    34441 taccacattg ctaccgctgc tctgtatcgt catgcttccg gcatggctgt tattagcagg
    34501 taagccgatg atgatggtca atcgtccgaa gcaacagcca ccccacgata atgtttagca
    34561 aaaaatgctc tcctttatga tttaagagtt atggcgatga tacggggctt tacctccccg
    34621 taatattgcg ttaacaggcc gctgacggct tcgatcgtga aggagaataa caatgttcag
    34681 gtcactgttt ctggcggccg ccctgatggc atttaccccg cttgcagcaa atgcaggtga
    34741 aatcacccta ctgccatcga tcaaattaca aattggcgat cgcgatcatt acggtaatta
    34801 ctgggacggt ggtcactggc gagatcgtga ttactggcat cgcaattatg aatggcgcaa
    34861 aaaccgctgg tggcgtcatg ataatggcta tcatcgtggc tgggataagc gtaaagcata
    34921 tgagcgtggc tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa aaggccgcgg
    34981 acatgggcac cgccattaaa cgcgtcctcc aatggagcac aatgcctgat gcgacgctag
    35041 cgcgtcttat caggcctaca aaccggctct ttaacgcgta gggcggataa ggcgtttacg
    35101 ccgcatccgc caatagtgcc agatgcgacg ctcattcaac tacaatccca gcgccgtccc
    35161 caccaacaac cagatattca gcgccacgac cagcactaca atcacccagc ctgtctgttt
    35221 tacgcgtttg ctgttcacca gatcgcccat caacttgctg tcactggtga aaatcagcag
    35281 tggaaccagc gccagggcga taccaaaact taacagcacc tgactcataa ccagaatccg
    35341 tgtcggatct aatcccatca gaatgacaat aaatgacggc aacatggtga ctgtacgacg
    35401 cacccacagc gggatatgga agcgaatgaa tccctgcatc accacctgcc ccgccagtgt
    35461 ccccaccacc gttgaggaca gtccggcagc aaccagactt aacccaaaga ccgttgccgc
    35521 agcatggctt aacagcggtt gcagcgtcag ataagcctca tcaagatcgg caacaccagt
    35581 atgaccagaa aagtggaacg ccgccgcagc tgtagccatc atcaccagat tgacaaaacc
    35641 ggcaatcgtc atggcgatag ccacatccca tttggtggcg gaataacgtt gttgacgcga
    35701 accgccatgt aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt
    35761 cgcccctaac acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac
    35821 cattccttta cccagctgcg ccaggttagg ctgggagaaa atcaactcga caatgtaagc
    35881 cgcggcaaca aacaacagta acccgccaat cactttctcc agcggttttt gcccgcgacg
    35941 ttgcagcatt aaaatcagga aagtcgcgat ccccgtcagc accgcgccct gcaacaacga
    36001 aacaccaaga atgagtttaa aaccgatcgc cgcaccaata aattccgcca ggtcggttgc
    36061 catcgcaata atttctgcct gaacccaata gaaccacact acgggacgcg gatagtgatc
    36121 acgaatctgc tccgccagat ttttaccggt ggcaatccct agtttggcag agaggatctg
    36181 aatcagcatc gccatcaggt tggcccaaac gacaacccac agtagctgat agccgaagct
    36241 agcacccgcc tgaatattgg tcgcaaagtt accgggatcg atataaccaa tcgccgcaat
    36301 gaacgcaggt cccattaatg cgagcctcat cttacgcgcc gcccgtccgc tgctactctc
    36361 aacgcgatag ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt ttcatgctat
    36421 gctaaacgag aatgattacc aaattcattt aaatggattg tggtgatttc tctgatagac
    36481 caagattatg attacgaaaa gattgatgac ttgaatgtta tggttatgtt taatgttagc
    36541 acatttacat aactttcagc ttccatacac aacatagcag aaatgtatga cagatcacta
    36601 tttttgaagc ctgtcacagg acgtcattat agtgtgtgtc agatctcgtt ttccttaacc
    36661 atgttacata gaatgtgcac ggaaatttaa cctgcctcat atttggagca aatatggacc
    36721 gcgtccttca ttttgtactg gcacttgccg ttgttgtgat tctcgcactg ctggtaagca
    36781 gcgaccgcaa aaaaattcgt atccgttatg ttattcaact gcttgttatc gaagtgttac
    36841 tggcgtggtt cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa
    36901 tgttcgaaaa actgctcgga tttgccaacg aagggactaa cttcgtcttt ggtagcatga
    36961 atgatcaagg cctggcattc ttcttcctga aagtgctgtg cccaatcgtc tttatctctg
    37021 cactgatcgg tattctccag cacattcgcg tgttgccggt gattatccgc gcaattggtt
    37081 tcctgctctc caaagtcaac ggtatgggca aactggaatc ctttaacgcc gtcagctccc
    37141 tgattctggg tcagtcagaa aactttattg cctataaaga tatcctcggc aaaatctccc
    37201 gtaatcgtat gtacaccatg gctgccacgg caatgtccac cgtgtcgatg tccatcgttg
    37261 gtgcatacat gaccatgctg gaaccgaaat acgtcgttgc tgcgctggta ctgaacatgt
    37321 tcagcacctt tatcgtgctg tcgctgatca atccttaccg tgttgatgcc agtgaagaaa
    37381 acatccagat gtccaacctg cacgaaggtc agagcttctt cgaaatgctg ggtgaataca
    37441 ttctggcagg tttcaaagtt gccattatcg ttgccgcgat gctgatcggc tttatcgccc
    37501 tgatcgccgc gctgaacgca ctgtttgcca ccgttactgg ctggtttggc tacagcatct
    37561 ccttccaggg catcctgggc tacatcttct atccgattgc atgggtgatg ggtgttcctt
    37621 ccagtgaagc actgcaagtg ggcagtatca tggcgaccaa actggtttcc aacgagttcg
    37681 ttgcgatgat ggatctgcag aaaattgctt ccacgctctc tccgcgtgct gaaggcatca
    37741 tctctgtgtt cctagtttcc ttcgctaact tctcttcaat cgggattatc gcaggtgcag
    37801 ttaaaggcct gaatgaagag caaggtaacg tggtttctcg cttcggtctg aaactggttt
    37861 acggctctac cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg ctgtaaaacc
    37921 atacataaaa aagccgggga tcattccccg gcttttttac gcctgttaat cagctaatgg
    37981 ctgcgggcga ccaatcaaat acccttgcag atattgcacc ccgagcttat gcaatagcgc
    38041 ctgctgctgt tgcgtctcga caaactccgc gaccacactc aatgacttcg ctttcgccag
    38101 atcggtaatt gatctcacaa tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa
    38161 gacgccatca attttgatga tatcagcctg caaacgcttt aaccgttcgt agttggcata
    38221 tccggtgcca aagtcatcaa tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt
    38281 gtacatgctg ctttctgcgt tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc
    38341 ctgcggggag atgtgataac gcttaaacag acgaataatc cgcccggcaa tattcttttg
    38401 cagcagcgtg agcggcatta aattgactga aaagcgcggt ccttttttgt cgcaagggtg
    38461 tgttgccagc cacttcaaca aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt
    38521 aaactgagca ataaggggca gaaacttatc cggggtcata atgccgccgt catatttcag
    38581 tcgcgcgagg atctcatcat aaccttcacc ctctttgttg cgaattggct gggcgtagag
    38641 cagcaaatca ccctgatcta acgccgtgcg aatggtattc agcaatagca cctgtttagt
    38701 ggtctgcccg gaaaccatct cctctctgct atccagcgcc agcacatgat gatgtgcgca
    38761 ggattgctcc gccagccagc ttaactgccc caacaagggt tgcagggttt cctgatttcc
    38821 atcaaaacgc ccccaggcag caccatagcc catatccagc ccggtattgt tccagtgaat
    38881 ttgccgacta ttcaggatgt taaccatatg ctggagtcgc ccttccgttt ccggcccgct
    38941 cagcactaac agcagttcac tacccggcaa ttgatacaac ttttcgtttt cctgcatcaa
    39001 cggcagcagc gtacggcaaa ttgagcggat acagtgaacg cgcatcatta agccgtaatg
    39061 acgactcata aactcaagat tatcaatgcg caggcagcaa aaactcttgc ccgcctcttg
    39121 ctccggcgct tgttccaacg cacgaaagtt gggtaggagc gttaacggat ctgttagcgc
    39181 ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca ccatatagag
    39241 caggcaaacg ctaaaggaaa tcagcaccgc gagaataaat gccagcgaat attcggtttc
    39301 caccccttgc aaaaagttct gattgtaatt cagaaggcaa agcgttgaaa cagcccaggt
    39361 gagatttaaa aacggatagc gaagattacc gaccccgagg gtaaagatga tgaagaaaac
    39421 gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca gaagtagcag
    39481 cacgcttaac gctgccagcc agcttaaggt aaatgcgagt gagatagtag aaaagcatgt
    39541 tgtaaatcag cactgcggtg aaaaggctta gtaaatcaac gaccgtgaaa atggcatccg
    39601 catcgccgaa aaaggtggat atctttagcg ggaaatcaaa gaagctcccc acaagataca
    39661 tgctgcattt gatgccgatc ggcgtcacca aaccaagcca gaacagacgt tgccagacat
    39721 atcggctggt caatccataa cgccaacgcg tccccagctg ccagcgtaat attgcgcaag
    39781 cacacaacac gacaaacgtc tggctgccga gcatgaccgc agtctgtaaa aaagagagat
    39841 gaaagttcca cagattggtg caaaacatcc cgcataaaac gggaacgaca ccgcgccagc
    39901 caaagataaa caagattgac agcattacgc acagtggcat ccacgccaga aagatataac
    39961 tggaatggat aatggcaagt ggcgaaataa aacgggatag ctgaataagt accacggtga
    40021 gcgtaaacgc tagtgtgaat atcttgatat tttttatcag gttatgctcc acaaacatga
    40081 gtacactact ctcactgatc ggggttttca ggcacacaaa taataagttt attcgcatcg
    40141 cgaagcaaac aggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
    40201 gcgctaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
    40261 cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
    40321 tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca
    40381 aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
    40441 gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga
    40501 cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt
    40561 caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta
    40621 ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata
    40681 cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac
    40741 cggctatagc cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc
    40801 aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga
    40861 atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga
    40921 tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa
    40981 acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat
    41041 tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg
    41101 aatgcctcgg tgtggctaaa caaactgtta accgttggac cagagaaaaa ggctggaaaa
    41161 cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag
    41221 tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg atggaagctg
    41281 aagagagaat tgctgaatac gctcctggag cacgcgcccc ggcgtatcga caaatcatca
    41341 acgcgataga taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg
    41401 aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg
    41461 gcaggatatt atctcctgcc gtttatcttt ttacacgcta attactgctg attttcgcgt
    41521 tcagcaataa aatccagcgc tttgttgata cgctcgatac tgcgggtctt accaattgcg
    41581 tgaacggtaa catccagtgc tggagactgc cccgcaccgg ttacggcgac acgcagcggc
    41641 ataccaactt tacccatacc cacttccagc tcatccgccg tcgcctgaat agcgtgatga
    41701 acgttttcag cggtccagtc agtaatcgcg gccagtttgt cacgaaccac ttccagcggc
    41761 tgacgcgcta ccggacgcag atgttttttc gccgcatcgg catcgaactc agcaaaatct
    41821 tcgtagaaat aacggcagct ctgtgccatc tctttcagcg tcttgcagcg ttcgcccagc
    41881 agtttcacca gatcagccag ctgcgggccg ttacgggtat cgatattttc ctgctcaatg
    41941 tgccactgta agtgagtagc aacatactcc ggcggcagcg cgttaatgta gtgatggttc
    42001 agccacagca gcttgtcggt gttgaacgca ctggcagatt tgctgacggc attcaaagtg
    42061 aagtatttga tcatctcttc acgagtgaag atttcctgat cgccgtggga ccagcccaga
    42121 cgcaccagat agttcagcag tgcttctggc aaataaccgt catcacgata ctgcattacg
    42181 ctgactgccc cgtgacgttt ggacagtttt ttaccgtcat cgccattgat catagaaacg
    42241 tgcgcgtaaa ccggcaccgg cgctttcagg gccttaagaa tgttgatctg gcgcggcgtg
    42301 ttgttgatat ggtcttcgcc acggataacg tgggtgattt ccatatccca gtcatcgaca
    42361 accacacaga agttataggt tggggaacca tcggtacggc ggataataag atcgtccagt
    42421 tcctggttgc tgaactcgat cggaccacgg atctgatcgt caaaaacaac agaaccttcc
    42481 tgcgggttag caaaacgtac aacacacggt tcatcatcag catgatgctc atggctgtgg
    42541 cggcagcgac cgtcataacg cggcttctca cctttcgcca tttgctcttc gcgcagcgct
    42601 tccaggcgct ctttagagca atagcattta taagcagtgc cctcttccag catctgatcg
    42661 atcaccgcgt tgtagcgatc aaaacgtttg gtctggtagt acggaccttc atcccactcc
    42721 aggctcagcc agttcatgcc atccataatg gcttcgatag cttccggcgt ggaacgctca
    42781 agatcggtgt cttcaatacg cagcacgaac tcaccgccgt ggttacgtgc aaaaagccag
    42841 gagtaaagag cagtacgcgc gccgccaacg tgcagatagc ctgttgggct tggcgcgaag
    42901 cgagttttga ttttcatgaa atggccttac gtttagaaag atgccgacaa ccggcaaatc
    42961 ctggaaaaat tgagtgggcg atattctatc actcccgcct gattcctcaa tgtagttcgg
    43021 gtttctacct tacgcattat tgttttcgtt gacaaattgc gcaacacggt ctgttttgcg
    43081 atcatcttgc ttaaatttac gacgaacgaa caatttcttt agaaaatgcg ttgactcatt
    43141 ttgaactctc cctataatgc gactccacac agcgggggtg attagctcag ctgggagagc
    43201 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta
    43261 tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc
    43321 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc
    43381 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
    43441 acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatactcaa
    43501 agattcgaac ctgcagcagg tttgagttga gcgcagcgaa acaacggagc cgctcgcggc
    43561 gacggcccga agggcgagcg aagcgagtca tcctgcacga cccaccaatg caaaaaagcg
    43621 ccctaaaggc gcttttttgc tatctgcgat ttgtaagatt gatttttctt ctcaagttcc
    43681 ttgttcttct accgatatta cctcgtacag tgacgaggac tgaacaatgg ccactatctc
    43741 ttcaacttca attccttcca tacaaacaca gagtagtaac agggcatcac aaggtagcga
    43801 tgttgcttca caaatcgcgc ggataagtca gcaaatcatc aaactcactc aacaaattaa
    43861 agagattgta gacactagcg gcagtgctga agacaagcaa aaacaggccg aattgattca
    43921 acagcaaatc accctactgg aaacacaact ggcgcagttg cagaaacagc aggcagaaaa
    43981 ggcgcaggag aaagagcagc gtttgtctct gaatgtatcc cttctaaatc ctgttgaaaa
    44041 aacgactcat atcgatatct atatctgata caacggatcc ccttcccgac cagccattag
    44101 cgcattgccc tggtctcgta ccacgtccca taacgcttca gcggcggtgg aaagtgaacg
    44161 gttcttcctg cgcaccagca tcaactggcg ttcaaccacc ggcgtaatgc gcttaaccac
    44221 cagtgggcta ccttcaggca atggtagcgc cagtgcagga agaatactga tgccgatgcc
    44281 tgcggctacc atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa tattcgcctg
    44341 aataccattg cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg cgtaatcctg
    44401 caacaccaat ttcgctccct gtaatgcctg ccagggtaca taatcttcca cggccaaagc
    44461 gctatcgcga tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac attgcagatc
    44521 gcccactggg cctggatcga tgacgatgcc aaagtcgaca tctccctggc gaatactttc
    44581 catcacccac tgctgcggac gatcatgcag gacaaactga atatctggat agcggcgatg
    44641 gctttcagca atgcattgcg gaataagatg cgcggaaatg gtctggctgg cagcgacccg
    44701 aacttttccg ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc tgttaagttc
    44761 atccagcagt cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa gcaccacttc
    44821 acgcgtggtt ctgtccagca ggcgaacacc agtatgattt tccagctcct tcacactgtg
    44881 acttactgcc gactggctca agccgatacg ctctcctgca cgactaaaac ttttctcctg
    44941 cgctactgtg acgaaaacct ttaattgttt taaagaataa ttcatctgtt ttcttcatga
    45001 atggatgcaa taaatcaatt ttatttctca aactggaaga agcacaatag aaccatcgat
    45061 catctggagt ctttatgaaa ctttttcgta tcctcgatcc tttcacctta accctgatca
    45121 cggtggtgtt gctggcctct ttctttccgg ccagaggcga tttcgtcccc ttctttgaaa
    45181 atctgaccac cgcagctatt gccctgctgt tctttatgca cggcgcgaag ttgtcgcgtg
    45241 aggcgattat tgctggcggt ggtcactggc gactgcattt gtgggtaatg tgcagcacct
    45301 tcgtgctgtt tccgattctg ggtgtactgt ttgcctggtg gaaaccggta aatgtcgacc
    45361 cgatgctcta ctccggtttt ctctacttgt gcattctccc ggctaccgtg cagtctgcaa
    45421 tcgccttcac gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct gcgtcagcat
    45481 ccagcctgct ggggattttc ctttcaccat tgctggttgg tctggtgatg aatgttcacg
    45541 gtgcaagggg cagccttgag caggtcggta aaattatgct gcaactgctg ctgccgtttg
    45601 tgttggggca tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat aaaaaatgga
    45661 ttgcgaaaac tgaccagacg tccattctgt tggtggttta tacagcgttc agcgaagccg
    45721 tcgttaatgg tatctggcat aaagttggct ggggatcatt gctgtttatc gtggtggtca
    45781 gctgcgttct tctggctatc gtgattgtag ttaacgtctt tatggcacgc cgactgagct
    45841 tcaataaggc agatgaaatt actatcgtct tttgtggttc gaaaaagagt ctggcaaatg
    45901 gcatcccgat ggcaaacatt ctgttcccca catcggtgat cggtatgatg gtgctgcccc
    45961 tgatgatttt ccatcagatc caattgatgg tctgtgcggt gctggcgcgt cgatacaaac
    46021 gccagaccga acagttacag gcgcagcagg aaagcagcgc cgataaagct taaagcggac
    46081 gcttcagggg ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc gtgatttgca
    46141 ttagctcacc gagcttccct gccgggaact catctttgcg ggcaaaccac agcagatact
    46201 cctccggcag gtcaattaag cgacgccctt tgtatttgcc aaacggcatt atcgtattgg
    46261 ctatttcaat cagctgctct ttttccatct cagctaccca gcaaacgcag catttccgct
    46321 tcgtcgatga cttcaatgcc cagttcctgc gccttcgcca gtttagatcc tgcagcttca
    46381 cccgctatca ccagatcggt tttcttcgac acgctgcccg cgactttcgc gcccagttcg
    46441 accagtcgag ctttagcgtc atcacgcgac atctggctta agctgcccgt aagcaccacg
    46501 gttttaccag caaacgggct gtcaatctct tccgcgttga taacgatcgg cgcaggccag
    46561 tgaacacctt ccgccaacag ctcgctgatg acattgcggt tgctttcttc ggcaaagaag
    46621 ttgtgaacgt gggatgcaac gacaatgcca acatcaggca ccttttgcag ctcttcaatc
    46681 gaagcggctt ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc tgcggtggcc
    46741 tcgccgactt cacggatgcc aagtgcatag aggaagcgag caaaggtggt ttctttcgct
    46801 ttttccagcg cgttaaccac gttttgtgcc aattttggcc ccatacgctc cagtccggtc
    46861 agtttgcctg cggtgagttt gaacagatct gccggagtat ggacatattc tttttcaacc
    46921 agctgatcga tgattttgtc gcccattccg tcaacatcca tcgcacggcg ggaaacaaag
    46981 tgtttcagcg actctttacg ctgcgcaccg caaatcaggc caccggtaca gcgggcaacc
    47041 gcttcacctt ccacacgctc aacgtcagaa ccacataccg gacaatgcgt cgggaataca
    47101 acctcacggg tatcttccgg gcgttcagaa agcacgacgt taaccacctg cgggatcacg
    47161 tcgccagcgc ggcgaatcac cactttatcg ccaatgcgta aaccaagacg ttcgatttca
    47221 tccgcattgt gtaaggttgc gttactcacc agcacgccag caacatggac aggttccaga
    47281 cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc acgcacaaag
    47341 gtcatctgct cctgcgccgg gaatttaaac gcaaccgccc agcgcggggc acgcgcgaca
    47401 aagccaagct gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc gtcgatatca
    47461 aagcccagcg tcgggcggtc ttcttccact ttgtgataga acgccagcac ttcttccgcc
    47521 gattcacaaa gcgttacccg atcgctgacc ggcacccccc actttttaaa ttgcagtaaa
    47581 cggccaagat gagtatccgg cagctcgcca ccttccagaa caccaacgcc atagcagaaa
    47641 aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga accagctgcc
    47701 gcattacgtg ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc gttaatcttt
    47761 tcgaaccccg cctgcggcag gaacacttca ccacgcactt ccagacgcgc cgggatattc
    47821 tctccgtgca gcttcagcgg aatggcgcga atagtacgca cattagacgt gatatcttcc
    47881 ccggtggtgc catcgccacg ggtcgcggca ctgactaaaa cgccattttc atacagaata
    47941 ctgacggcaa ggccatccag cttcagctca cagcaccagg tgactttctc gttgtttttc
    48001 agacggtcct gcacacgttt gttgaaagca agaaagcttt cttcatcaaa aacgttatcc
    48061 agtgacagca ttggtacttc atggcgtatc tggctgaaag ctgccagcgg cgcagcacct
    48121 acacgttggg taggcgaatc aggcgtaatc agttctggat gtttggtttc cagctcgcgc
    48181 agttcgcgca tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc catcacatga
    48241 taaagatatt catgatggca aagcgtcgtt cgcagttctg tcagttgttg ttcgattgat
    48301 tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa gaggagttaa
    48361 tttgccttaa gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt cctggtactc
    48421 gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca agcacgacac cgcccacttc
    48481 atcggcaata tgctgcgcag attgcagcat cagcttgaag ttctgcagct cgtcaccgta
    48541 agacggtacc tgcataaaga tagtgacacc cggagtagtg aaatccttca tttcaggatc
    48601 aaaggttccc ggtttcacca tattcgccag gctgaataac gccgggccgc tgccatccgg
    48661 gctaagatga cgatggtaaa tattcatatc gccaaaaatg aagcccgctt gttgaatgct
    48721 gttaagaagc agttcaccgt ttagctcgct accgtgatgc gccgcgacgt tcatgataat
    48781 caccgcttct ttgcgcttcg gtttatccat aactggagcc ggttccgcta caggctcagg
    48841 ctgtggtgcc gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt gcggtgctga
    48901 atgcacaggc tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca acggctgttc
    48961 aggctgcggc tgataggcag gctgctgcac cggctgcgct ggacgcggag catgttgcgg
    49021 cggtacctgc gcttcaggcg gctgctggac cggttgacgc ggctgcgcag acgcataagg
    49081 cggtaggtac tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg
    49141 ggcatgattc acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc
    49201 gtcataagaa tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat
    49261 agaagatcgt tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat
    49321 cgcgccaaca atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct
    49381 aacaccttgc caccacggca aacatttact cactaagagt atttgccgat tacctcaagt
    49441 gcaagtgcac tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt
    49501 tttcgaacat atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc
    49561 tgggattggt caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg
    49621 cagcggtttt tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg
    49681 tttcgttatt ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct
    49741 ctttacacag ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca
    49801 atggctgagt tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta
    49861 tttcttctcc acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca
    49921 actggaagca cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa
    49981 agatgtgccg cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc
    50041 aattgtatta ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact
    50101 gtggttcctg tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa
    50161 ccacaaagtg ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat
    50221 gcagtttggt gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat
    50281 gcccgttgcc gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc
    50341 gatgtggcgg taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc
    50401 ttatgccgcc ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct
    50461 aaatccttac ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat
    50521 acagttaagg acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca
    50581 cgccgctggt tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc
    50641 gtaaccccag cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa
    50701 agcgcggcgt gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga
    50761 ttgcactggc ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa
    50821 ccatgagtat tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg
    50881 aaggtgctaa aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc
    50941 cagagaaata cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa
    51001 aaaccaccgg tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg
    51061 gcgttgggac tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca
    51121 agaccgatct tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc
    51181 tggcaggtga agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta
    51241 tcccggctaa cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag
    51301 ccatttctac cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg
    51361 gagcagctgt tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata
    51421 ttgtggttat tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc
    51481 tcttcactga gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca
    51541 cctttttagg tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca
    51601 gcctgtcgga actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac
    51661 aggaaaagcc aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata
    51721 atgtttaatg acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg
    51781 ctcttcgtgc gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg
    51841 ccaggctaga ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca
    51901 gcaagaagtt accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt
    51961 aaaagaagct aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag
    52021 cgcgaaaagc ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat
    52081 ctccgcagaa ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga
    52141 actcgagtaa tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt
    52201 tcaggcattt tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac
    52261 gaaattgtca ttgaccggaa aaaaatttct gccgaccagg ttgatcagga agttgaacgt
    52321 tttctgagcg gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt
    52381 gaaacgttcg gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat
    52441 gaggagctgg agcaggaaat catagccctg attaaagata aacacatgac agctgacgca
    52501 gctgctcatg aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac
    52561 ctgaaagaac gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg
    52621 ggcctgaaga ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac
    52681 ctgacgccgt ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac
    52741 gcgggtggcc gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc
    52801 gtgggtaccg gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc
    52861 gtaaataatc aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt
    52921 caggagcaag tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt
    52981 acgctggacg gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa
    53041 ggtgcagagc gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg
    53101 gaccgcgacg cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa
    53161 gcgtgtggct cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg
    53221 ccatacatga acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc
    53281 gcgatggatc gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct
    53341 ttcggtaaat tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg
    53401 cgcaaagaga tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag
    53461 tcaattgaaa tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta
    53521 gccaaagaag ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca
    53581 gttgaccgtg gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg
    53641 aacttgatca agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt
    53701 ggtgagcttg ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa
    53761 ttctctatga gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc
    53821 gaagatgcga aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg
    53881 acgctggtta acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca
    53941 atttactgct taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga
    54001 cgacaaaaag gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa
    54061 tatcgaagac gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat
    54121 caaaccaacg ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga
    54181 aaccaatcac gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg
    54241 tatcgacacc gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg
    54301 cgtgaaagtt ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa
    54361 gtctaccctg actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact
    54421 gtccggtagc gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc
    54481 gcagtgaaaa atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat
    54541 caattcatcg ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc
    54601 acggtgcacc gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca
    54661 aaacaggtcg ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac
    54721 gctgtcggca ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct
    54781 ggtaatcacc acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact
    54841 gtcgagatcg cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat
    54901 tccctgcgcc agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata
    54961 aattccgcta tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc
    55021 tttgcgtagc gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata
    55081 gcccgtggtt acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata
    55141 gccgctaaac cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt
    55201 gctcagcaat accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac
    55261 cgcaatgctg ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc
    55321 ctgcagcgcc ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct
    55381 tgccgatgat cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg
    55441 ctattacaaa acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc
    55501 tgctaatgac actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg
    55561 ccatgctgga gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac
    55621 atacctgcga tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca
    55681 ttgacactgc cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc
    55741 cgtatatcgt gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt
    55801 taattgcgca gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc
    55861 atttacagga ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt
    55921 ttggtggata gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat
    55981 tggtaaattc catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa
    56041 attgattgcc gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga
    56101 tctctactat tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt
    56161 ccagtgccat tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg
    56221 tagcgatgaa ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg
    56281 ctgatttccc tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca
    56341 gtattcttga agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga
    56401 gcaatatgat tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc
    56461 ataaacttaa aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc
    56521 ttcaatgggg tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactg
    56581 cttttccaca cgaaaattaa aaactgcatt tgtcggcagc aacaactgcc gacaaatcag
    56641 attattgaca aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata
    56701 aaaatattgc taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc
    56761 aggcagtttt gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct
    56821 gactaaaatg ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga
    56881 tgatcgctac caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg
    56941 cgccgcctga gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt
    57001 tttccgcatc gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc
    57061 ccggcagata ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt
    57121 gcaggccaac aatggtcacc ggtttggatt gttcgcgcat aaagcatgaa acgccggtga
    57181 tagtgccggt cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc
    57241 agatttccgg cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat
    57301 cgagcagctt tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca
    57361 tgccctgctc tttggtgaca agaatcagtt ccgcaccata agcacgcatc gccgcacggc
    57421 gttcctggct catgttgtcg ggcatcagca atttcatgcg atagcctttc agcgcggcaa
    57481 tcattgccag cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt
    57541 taatttcccc gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg
    57601 aacctgccgg gttattgcct tccagtttta accacacttc actgccgtta tccggcccca
    57661 ttcgctgcaa cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacaatct
    57721 ctatccatac gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta
    57781 caaaccttgc cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac
    57841 tcaacctatc aggcgctttg tgcgagagca agttcctcat cgcgggtttc aatacgctcg
    57901 tcgccgttat acagccgcgc atgttgcaga ccaacgaata aacgctcgcc atgctgcggg
    57961 gcatcgtcgc catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc
    58021 actaattggg tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa
    58081 tcgagactgg tacggcggct gatatccact tcccacgggc gcaggaagag atccaccggc
    58141 ccctgatacg caggtgtgta gcccagcggc cagcgatgcg cgccaacatg gaactgcccg
    58201 ccgcgaatgg ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc
    58261 gctggttcgc gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc
    58321 accactacac gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg
    58381 gtgaatttca gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc
    58441 gcatccagcg cgccgaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg
    58501 cgcgccagcg ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc
    58561 agatgcgcaa gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca
    58621 ttcgggcgct cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc gaataccgtc
    58681 atatggcgga acagcgcgta atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc
    58741 aggcgactca cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg
    58801 gcgataatcc gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc
    58861 tgaccagaag gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaaagacttc
    58921 ttgatattgg caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt
    58981 ctccaggcgc cactgcaaca tactctttaa aaacagggtg ataatcgcca tcaacgtcaa
    59041 cagcgcggcg gcggtaaagg agccgacggt gttgtagtcc tgctccaaca attcaatctg
    59101 taacggcagc gaaagtgttt cgccgcgaat cgagccggaa accaccgaca ctgcaccaaa
    59161 ctcgccaatc gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc agcgaatatt
    59221 cggcaatgtg acgcgacgga acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc
    59281 gtcttcctgg ctgccctggc ttaacatcac cggcaccagc tcgcgcacca caaacggaca
    59341 cgtcacaaag atggtgacca gcaccattcc gggccaggag aacataattt gcagattatg
    59401 ttcatccagc cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc
    59461 ggcaaccacc ggcgatacag caaacggaat gtccagtagc gtcagcagta actggcgtcc
    59521 cgggaagtta aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg
    59581 tacggcaatc agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc
    59641 cagattctgt aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat
    59701 cggcaccagc aggatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc
    59761 ccagttaatc gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct
    59821 accacacgcc gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat
    59881 gccgcgagga tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc
    59941 acaaaaatca tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc
    60001 accgcgccaa attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc
    60061 ggagaaagct ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt
    60121 tccgccgctt cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc
    60181 acaaacggaa tgctggtaaa ggccatcgcc accgcaatac caagccaggt ataggtgact
    60241 ttgatatcaa acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc
    60301 agcgttaaac cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc
    60361 agcgtgcggc ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag accgaaaacg
    60421 ccgttaaaaa tcgatgccac aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc
    60481 tgcgggttgg tgatcacctc ccagtactgc gcccagctca tctcagaaag ctgcatcacc
    60541 agcgcagaga gcggcagcag caatatcagg cacacaaaca gcagactggt gccaaggctt
    60601 aaagtaaagc ccggcagcac gcgtcttgaa gaaactgcaa acattactta cgccccgccg
    60661 ctaacagctt gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccatg
    60721 agccaaattt gtcttccacg cggaacagtt cggtctgcgg gaatttatct ttcagtttgt
    60781 ccatgacttc cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg
    60841 ggctgtagag ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat
    60901 ttttatcgac ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt
    60961 caaagccctg tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca
    61021 gcacatcgcc caagccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga
    61081 acacttcaac gtttttcagg aactgggtca taaactgttc ggttttggct ttatcgccac
    61141 cgtcagcttt atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg
    61201 ttttcgggtt cgggaaaatc agcttcacgt cggagcgcac caggtcgttc cagtcgtgga
    61261 tattcttcgg gttgccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat
    61321 tattcggcag gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg tgcaggattt
    61381 gtacgtcggt cacctggtta taagtgacaa cgtctgcttt taagccctgc aaaatcgcca
    61441 gcgcctgttt tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat
    61501 cttttgccca ttgctgttca aacggcggat tcagggcggc aaacagctcg cgggagacgt
    61561 cataagaact gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga
    61621 ccagcgcgag tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta
    61681 atgactttct aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata
    61741 ccgtttggtg atttggaagt taaaaaggga ataagaactt ggttcaacag gcgtaattgt
    61801 tgcaggcagt ttggacacgg acagcgcgga gaaaccggag cgtacacata gtacgtgagg
    61861 atttcgagca ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa
    61921 gacgaaatcc tccccacaca tgcagggagg aaacagaggt gaatcagata ccgacgctaa
    61981 ccgtctccgg cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg
    62041 attcatccga tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac
    62101 acagcgggat tgcttttgcc atttcagtca gcaccgattc tggatcttcc gggttcgact
    62161 ggcgggcaat gctttccgcc attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc
    62221 gaataccgga ttgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt
    62281 tcgttaaggc atacgccgtt tcgccaggat cggccaccat atcaccagtg actgaagaca
    62341 tcatcacaat gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt
    62401 tccatacgcc tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga
    62461 ggaaactgcc cagacgacaa acgcctgcgt tattcaccag gatatcaatg cgcccttctt
    62521 tttccttcgc gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa
    62581 ccgccgtaca gcgatgacca cgaccacaca gttcgtccgc tagcttttcg atctcagggg
    62641 agatatccag caagattagg ttcgcgccat gacgtgcaaa agttctggca attccttcgc
    62701 caattccctg caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa
    62761 tgctctcctt gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca
    62821 aaatattgct gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat
    62881 gcatcatttc aggcgttgtg ttagctcact gctgcgctga atcattttca atgacgccac
    62941 atcattgatg ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc
    63001 gggtagacat cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat
    63061 gcgccagccg ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct
    63121 gctttcgtgc cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag
    63181 cgatttgtac atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg
    63241 cttcgcaggc cacgcgatag ccaattttca tcagtttgaa cgacagatcg cgccccacca
    63301 gcgccgatcc gcccaggccc gttatctgga taaacggtgc tttactgatg acctcaatga
    63361 ttttttgcag ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc agctcttttt
    63421 cgcgattcag tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca
    63481 ggtgcagcgc cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca
    63541 tacgcaattc agtaaatcct tgcgcgccga gcttctgggc aaatttcaca atgctcgact
    63601 ggctgatacc gagctgtttc gccatctgcc gtgaagagac ggatttcagc tcgctcactc
    63661 tggcccgcag aaagtcagca attttttgct cgttcccggt aaattcactt tccgcattgc
    63721 gtattttcgt caggtacaac atcttccact ctcctgggct ggcagcaaaa attccgctta
    63781 agttcttatg acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt
    63841 caattcggga tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac
    63901 caccgatgca actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg
    63961 accgcgtatc gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc
    64021 ctgccgttga tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg
    64081 tcagcggcgg cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc
    64141 tggatgccag cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga
    64201 ttgctggagg cgaatatgcc attcagcacg cggtggaagg agctgaagat agccgggaag
    64261 gtggtgttaa cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg
    64321 ctgccagcgg tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct
    64381 gccgcacagt gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg
    64441 ccattacacc gattgtaggt gccgaagttg ttaccggttc ttcgcggatg aaagcaggta
    64501 cagcgcagaa actggtgctc aatatgcttt ccaccgggct gatgattaaa tccggcaaag
    64561 tgttcggcaa cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg
    64621 tcaatatcgt taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg
    64681 cctgcgagcg caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag
    64741 aagctaaaaa acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat
    64801 aacccatggc caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg
    64861 gaccgggaaa tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg
    64921 acagttcact ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga
    64981 ccagcgacca ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga
    65041 tgagcgagct gctgggcgat gcaccggtac aggatgccgc agaaatcgcc gcccagaaca
    65101 aacgtcagtt aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca
    65161 ccatcttcac gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga
    65221 cgttaattgc cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc
    65281 tgaattttat gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt ctggtgggct
    65341 ataacgccgc ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct
    65401 ttttgctcgg ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct
    65461 ttggtctgcc catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgtgggcct
    65521 gcgcccgcat tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga
    65581 cctcgttaat caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg
    65641 gtggctggtt attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg
    65701 gttgtgcggt tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct
    65761 ttattcctgt ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc
    65821 tttcaatggc aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac
    65881 cgcacagtgc gctacgcagt caggtacgcg gggcgattat tcccggcctg ctgggcgttg
    65941 gtgaaccgct gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt
    66001 taggcggcgc ggcgggcggt ttgtttatcg gattgatagc ctggtggggt ctgccgatgg
    66061 gcttaaacag cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac
    66121 aaggcatcct tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg
    66181 ggtttatttt caccacgctc tttggctgcc gtaacgtcaa tctggactga tattatgaaa
    66241 cggacaatgc tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac
    66301 cctgttctga cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg
    66361 ccagtcagta atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcgcca
    66421 tcaacttgtc cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca
    66481 acatcgcatc acgtttgcca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt
    66541 tatgcagacg cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt
    66601 acggcaggct ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg
    66661 tgaggtgaga ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc
    66721 gcccgatcat catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct
    66781 cccaacgctg cacaaacaca tagctgccgc cggaatccac gccgtctttg ataaccgcca
    66841 cttcgcggcg cgtctcttca cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt
    66901 cacgctcttc cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa
    66961 aggcttccat cgccgcctga gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt
    67021 ggatcaacac atcgaactgg gtcgtcggcg caaggccttt accgtagccc ggaaaatctt
    67081 tcagctcttc tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa
    67141 ccaccgcacc aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa
    67201 atgttttact ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa
    67261 tcgccgcgcg gcaatgttct ggcaaaatgc cactctgaac ctgagacatt gttcctcctg
    67321 aaaataagaa tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc
    67381 gacggcggtt tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt
    67441 tttcagggta tcgtccgaag gcattaaccc ttcaggacca cgccattccc cgctgaactg
    67501 gtagctaata tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc
    67561 agctttttgg caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt
    67621 aacaccggtg tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg
    67681 atcgccgtta atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac
    67741 gttgccgttc gtggttttca ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc
    67801 aatggcttgt tcttgcagtg gtgtggacgc cgttaattca cccacgccct gctcgctcac
    67861 tttggtggac gatccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat
    67921 cgtggaacag ccggtcagca tcaacggcat agcgcataac attaaacgca gcgatttcat
    67981 gtcacttcct ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg
    68041 ttattctttt tcttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa
    68101 cgccacaata tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag
    68161 ccacagccac ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag
    68221 cacatgccag gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc
    68281 cggaataccg ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag
    68341 cagcgcagta ccctgctcac gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc
    68401 ctgaagcaat agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata
    68461 atggacagga tggaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca
    68521 cgtcggcgtg ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg
    68581 gcacgttgat ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa
    68641 gcaacgcatt ggcaattcca cgcccacgaa actctggatg cacgccaaga taataagcag
    68701 acccgcgatg cccgtcataa ccgcccatca ccgttccgac cacttcaccg tttacctcag
    68761 cgaccaaaaa caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt
    68821 tccacggacg cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct
    68881 ggcgaaatac gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta
    68941 tggcgtgaac ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat
    69001 ggggagttgt caactattga aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa
    69061 cggattacgc gatacgatat aacatctgga actttattat tacaactcag gccgtatgag
    69121 cacttttaaa ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttagc
    69181 tgccctgacg ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag
    69241 caacggacac agcaagccga aagccaaaaa atctggcggc aaacgtgtcg ttgttctcga
    69301 tccaggccac ggcggaattg ataccggagc gatcggacgc aacggttcga aagaaaaaca
    69361 tgtggtgctg gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc
    69421 gcgtttaacg cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca
    69481 taaacatggc gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc
    69541 tgccggtgct tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata
    69601 cctgtctgaa cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga
    69661 tcacctattg cagcaagtac tgtttgatct ggtgcaaacc gatactatta aaaacagcct
    69721 gacgcttggc tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa
    69781 caccgaacaa gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga
    69841 aacctcgttt atcaccaacc cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca
    69901 gaaaatcgcc acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca
    69961 gaaagcacat tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt
    70021 tctgctcaac cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt
    70081 tgtcgaagat agttggcagc gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa
    70141 tggtggtgtt ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc
    70201 tgcttccgcc accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt
    70261 ttcactggta gtgcatccgc ataacccgta tgttcccacc agccacgcga atgtgcgttt
    70321 ttttattgcc gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt
    70381 aacccctttc tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct
    70441 gtgccagcca tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt
    70501 ctacctcaaa catcgcaacg aacagcgcgg cattggcggg ctgttctttg atgatctgaa
    70561 cacgccagat ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga
    70621 cgcttattta ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa
    70681 ttttcagctc taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac
    70741 gctgtttggc ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact
    70801 ggtacgctgg gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga
    70861 gtttattaag gtcagggatt gggtgtaact ccctcacccc cattcccgca tccgctgatg
    70921 caacgtcaac gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag
    70981 atgccagaat ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct
    71041 tatcagttcg cggcgtacga cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat
    71101 gcctaaaata gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca gttgattaca
    71161 caaatccagc accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga
    71221 aagcaaccga cggtaactct gatgactgat gctttccgcc gtcaccattg gttgcgcttc
    71281 ttccagcatc gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg
    71341 ctgatggaga ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca
    71401 cagcgcggct ttatgctgct ctttcacttc cagcgccgac tggctacgca gcatatgtaa
    71461 tacccgatcc ggattgtgca aaaagttagc ctgacaggtg atgacatctt cagacagcac
    71521 tacgcccagg atcgtataat cgtccggcgt acttaattca aactcggttc caccggggcg
    71581 cgtcgctatt tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt
    71641 cgccggaatg ccaaaccaga acgagttcgg ccagaccagg cacgactgac gcagcgccag
    71701 accggtgtat tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg
    71761 aaacttgccc ggatgcagct gatcgtaaat ctgctgctag gcggtaatcg tcaaagcatg
    71821 ttcatagaca tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag
    71881 cttcaggttt tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt
    71941 tttcatgatg ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg
    72001 cttgagacga taacgactac tgcgtttacg taacgtcccg gcagaaaaga gctgttcgag
    72061 cgcatttctg gctttttcca gcggccagcc aaagtgggcg gccacttctc ctgccgtcat
    72121 tccctgacat actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac
    72181 tggcgcgtcg ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca
    72241 ctgggtgtca tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat
    72301 cgcggcagca caacctgcgt ccagcgccgc acgacacgcc gccagatcgc cttccaccac
    72361 cagcgttaag cgaccagggt caagcacttc gtgactgagc agacgaacgt tagctgcttt
    72421 gagcatggca tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag
    72481 tgcattgatc atcggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag
    72541 cactgcatcg gtaaaggcgt tacaggctgc tttacacgcg gcctggctac cggttaaaaa
    72601 tgctgccgag tagttggttt cagacggtgg cgggacgtag gtcaccagct gtacgtcggc
    72661 agatttcaac gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc
    72721 catcggatcg ccaagcgtga tcccggcggt tgatgaaaga taagaaccgg tacgcgaaac
    72781 cacatgcgcc aggaaggcgg tgttttctgc gtcattggcc cactggaaag ctgcgccatt
    72841 ttcaatatgt gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc
    72901 aagcataatc agcacttcac cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag
    72961 cgagcgacca tataccactt cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta
    73021 ggttacgtcg tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg gcggcaattt
    73081 tagttcacgc gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg
    73141 tcgaatcaaa tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc
    73201 tttctgctcc agcatacgtt tcgccaaatc cacaatgacg gcacagcagc acgaaagaga
    73261 agcaaaagtc agctacctgg tacactaggc agcgggtcag caaagggggg caaatgcccc
    73321 ttttttcatg tcagataatc agatcattac ctcaataaac taataatcac atcgcggtta
    73381 caaccagttt gatagtgata gctcaaaccc agacgtggcg ctggctgcaa gcaaaataag
    73441 ccgcaaaaat gcgaccccat gtttactagc cgcaaaaatg cggtcctatt tgccagaacc
    73501 ttttattatt caattggtta gtgcaagaaa aagcgtgatt gctgttcaag aagtatccag
    73561 aaaggtcatg attttgacgc tgaaaaaaaa taggtgggca tttttgccca ctcactagcc
    73621 gcaaatttgc ccactcacta gccgcatttt tgcggcccaa agtaataaat acagagaaat
    73681 aataaataca gatcagcgcc aagatttttc gtcttttcgg cggaatcatg agactagtgc
    73741 ttacgtcctg ataacctcca gagcagacct taacttctgt gcagcaggac tacgctcatt
    73801 gccgctcggc taggtcaaat cctcacgata aagctgttcg gattgtgacc ataatgttaa
    73861 aggactcttt aaatcattcc ttgacgaatt aactagctgg cttaagcgga atagcatgca
    73921 ggataaaaac aaaaccagct tagcagtggg gagagcttcg cttcgctcgt ctcctggggc
    73981 caatgctcaa tgaaacgacg taaggcacgg agcgctcacc ataggtagac acataaacat
    74041 ctgagagcgc accttctatc gcgctacgcg ctcaataaaa accaacgaca ccgccacact
    74101 gcaagaccaa tggtttttat cgcgtataca gaccagacag gaaacagcag caaagcagcg
    74161 tatagccagg tgcagacagc acactcacac gacgcacagc aggcttaagt gatcactcag
    74221 gaaactttat ccccgccgcc gatcaccgcg aactatcatg accgcgccaa attcgaaagg
    74281 aatggcccat agaacgcgat gaacacacat aagcgctaca cttacttaac gtcgccacag
    74341 agcgattgag agcgcttaaa atcgatgcga tttcattaaa tttttctcac tcgcctgaat
    74401 ggtggcgatc aggactgatc gcaacattgc cccgcctcga tattgagcgc ataatagata
    74461 catacattaa gcggaaaaga gataagttta ttgtgaaaat cagggggtta catatcatgc
    74521 gcatatcaga acaacaacgg cagatcctcg actatctggc aaaaaacgaa tcaatcaccg
    74581 cacgacaagc cgcagatctc gtttatggtg gcaatgttac ccgcgtccag gttgacgccg
    74641 cccgccgctc actcatgacg atggttagta atgggctaat caggaaacaa ggccgcgctt
    74701 tcgtagctga aagacatatc aatcacgctt atagccagga cgttgaagaa ttgcgccgcc
    74761 agatcctttg ggtattcagc aaacagggtc aggagtattt cctttctgaa cacccaaatt
    74821 tcgccgccaa agagttaaca gtaagcggat tgtcgtgcgc actggattat cttgtcggtg
    74881 aaggttgcgt tacttacaag cagctatggc acgccctgaa tacgcttgta gaagatggtt
    74941 tactcattgc cacgcgtgag aacatcgaat actggataat gccgcgttca ggtcgccgca
    75001 atcatgcacg gtatatttca ggcaacagat acagaacgcc ggactttaat ccgacacagg
    75061 aagatcggga ccgtgccgaa atgatgtgca gggaacagga gaaaagaatt atggataagt
    75121 tattttcgat gcagtgaaca caagcgggcg caatgcccgt ttttattgcc ttattccacg
    75181 cctgacgaat caatttgatc acacctgaga aaatgcatag ccagaagtta aggccgttat
    75241 attgcgattg ggagcgtttt aaacggtatc aatttaatta attctgatgt ggtgatttgc
    75301 aggcaaaaaa aacccgccgg agaagcggga aatagagaca tatcgttgta gttattaaca
    75361 gatcactttg aggtcgaggg cggctaaccc tcgctaatat tatcccgcta ttaccttgcg
    75421 ggtgcaaata atcttttatt gctttgatga tttaataaat agcacttttt gctaacaaaa
    75481 accgctgtca atatatggcc aaaaatgtca acagaaaaaa taaggtttat aatattaagt
    75541 atcacatggg taatgggttc agggaatcgg aatccaccgc ctcacggaaa ttcaaaattt
    75601 gcttcttctt tcctccgtgg gcggcttctt tttcgtatcg aggtatttat gaaagcaata
    75661 acattagaac agttcgcagc aatggcgaat gtatcgacca gaacgctgag gcgctggcta
    75721 aaaaaaccat caaccggagt ccctttgcca tttaccccac gcggtactcg ccaattattc
    75781 tgggagcacg aatgcaaaga atggttgtac agaacagaac gataataatc acgcatcaca
    75841 ttttgaggta aacatcatga ccaatatcga aagattagaa cgctgtgccg atattatgcg
    75901 acgcagatgg atttatgacc ctgaagcggg tttattgttt agccgtgaaa caaaagacgt
    75961 tatcaaaggc agcatgacca ataaagggca tttggttgta acggtacatg aaggtaaatt
    76021 caaagtgaca ctcacctatc agaaagcctg ctatgtatac acatacggcg cttatgacga
    76081 gacattatat gaagtgcacc acgttaattt gaacaaacag gataaccgca tcaagaatat
    76141 tcgcctgatg ccgaaagaaa agcaccgccg gatacataaa aacgtccgca aacttattcg
    76201 gctgggccac acacaatggc ttcaaccgca attaattgaa caatcactat aaacacagga
    76261 gtaaatacca tgcattcatt agacagttat tttcagcgca cgacagcccc taaatcagcc
    76321 gcccaggaac gccgtgagga attccatgag aaagtgatgc gttctgctga ttatattgcg
    76381 gacaagttcg tggaaacggt tcgtccactt gttgatgaag tagctgataa acttcagtct
    76441 gaaatgccgg aagatatgga aggcactgca aagcggcgac tcatttgcga attaagtcgt
    76501 cgttttggcg tgtcaataag cgcttttaaa taatcgaaaa aaggggggaa agataatgga
    76561 cgcatttgag gtactggcaa ataaaggcgc tgaactggta gcacagcgtg ataaaaccgc
    76621 caacgaaggg gaacgcgcac tgctaaataa acagattaaa gcgatccgta tggcccagtt
    76681 taaattaatc agtaatgaag ttatcgaaac aattaaaccg cagatcccac aaatcgttgc
    76741 tgacgctatt aaagccgccg gactggagaa acgcattgcc gggctaaaaa cgaacgcaga
    76801 gaaaatggag atgtacaaac agagcggtgc tccgaatccc gatgagtact tctcaccgga
    76861 tgttgagttt atggcgcagg ttgaggaacg tctgggtagc tgtctcaccg aggaacagcg
    76921 tcgctatttt gatggagtgg atagttcggc aggtattgac ctgaactcgt atttcggtcg
    76981 tgaaattgag cattttgatg ccgccccgtc tatgccggag ccagaaccag aaatggccac
    77041 cgatcctgaa gctgagcaac ggaaacgcgt attcaatgcg atagggattc ggtactgatc
    77101 ggataaaaga aagccgggaa ttaccccggc tgacttaaca atgtgaccaa tgaacaaatc
    77161 ggacaagaca aacaggataa gcagtctcga aacaatgttc gggaactata ttagcacaag
    77221 tccggagaaa atcatcatgt tggcactacg catcatgcag ggcatcgcta agaccctcgc
    77281 agaacatgtc ttagacctga aacattcgcc actgagcaaa caggccatga agcgccaaac
    77341 gcttcgtctg tgggctgaat attcgcttgg aactatcaat aaaatcatcg acatgaaatc
    77401 ggggccgagc aaccagagcg cggaggaaat ggagttcatc aggcgtttaa ttctcattcg
    77461 cagagacatt catagccagt tacacagcgt gggaatcgat attaatgatg ggacgggcga
    77521 ttaattttcg gacaagtact atgcgcgcgc gaggagctga acatgaagca agatattgca
    77581 gacaggctgg agatactcga aggccagcgg gcagaagcga aacaactacg taagcaggcc
    77641 agaagagcgc acaggaacaa cgaagcagag ttactaacca aatacatcag tttcaccaat
    77701 tactgcatct acgagtgtta caaggaagat gcagaggact ggctggattc attgccagaa
    77761 cagtactgag cagaataaca ccacgtcatg ttgttctgag gtgagcatat cacctctgtt
    77821 caggtggcca aatttagtgt gccacttcag catcaccgtt gaaatcacaa taacgatttt
    77881 tttgtaaata tgctctcaca tatgtaggaa tagaccaggt tggcattgaa acacatctac
    77941 catgaccata ataaagtaat tttttgatat gtttatggtt ttatattaat taaaatatat
    78001 ctaaatttga tagttttaaa attgcaaaat ccagattgtg tttgtaatgt ttaatctgat
    78061 tgtattgagt ggcgtttatt atgaaaaata aatcaggtaa ttcttgttat agagaagcag
    78121 tcgatattca tagctcactt gagctttcat ttatattgca taaatatcat ttgcgtgtag
    78181 atgagaagaa atctcgaaat gtattcagcg ccatacgtga tgagctcatc cttgatgggg
    78241 attcacgaca aaacttagct actttctgtc aaacatggct tgatgatgaa atccgtgagt
    78301 tgcttaattt agctattgat aaaaacatga tttataaaga tgaatatcca caaatggcgg
    78361 aacctgaagg tcgctgtgtg cgtatgcttt ccgattcgtg gagtttcccc gattcgcgac
    78421 acaccttagg ttgctcaacc attggttcat ctgaagctgc tatgctcggc cgcttggcgt
    78481 taaaatggca gtggtgtaaa aaacgcgaag tacaaggtaa atcaaccgaa cctgatttgc
    78541 gatcctgtgc aaatatgctg gcataaattc tgccgttact gtgatgtgaa attgtgtgaa
    78601 attccacttg atgatcatca cctgataatg aatgctaaag aggttttgaa gcatgtcgat
    78661 gaaaacacta ttggtgtcgt tacgacttta ggtattactt atttttgtaa agcagattgc
    78721 gttagtgttt ttaagataag tgttaaagtt aatattagtt tttatattta catcgtaatt
    78781 atatgtttct attttaaaaa tgttcgagag tataaaaatg tctaatgcta atatcgcacc
    78841 caaaagtaag aaaatgacag tggggacact cgctatcatg aatataactg ctgtagtaag
    78901 tctacgattt ataccttctg aagcagaata cggcttaggt gccatttttt actatacctt
    78961 tgctgcaatt atgtttttag tacctgttgc ctttgttgcg gctgagctag ctacaacata
    79021 tccacaaaaa gggggggcat ttcgatggat aggagaagca tttggggctc gctggggttt
    79081 tgtaggaatg cttatgacct ggctacaagt catcccctac tttccaacag tactgacatt
    79141 tggtgcagtc tcggttgcat ttattgatgt tgatatgggg gtggcagaat caattgccag
    79201 taataaatgg tatattttat ttttcgtact atttgtttac tggggagcta tttgtgttgc
    79261 attacgtggc gttggtattt tctcgcaggt atctaagtgg tgcggtatta tagggacaat
    79321 tattccagct attatccttg ttattcttgg tttctcctat ctgtggttca gcggtaaacc
    79381 agcggtgatc ccattaggtt ggggtgatct tttccctaat atagctaact tccagaatat
    79441 tgttcttgcc gccagcatat ttcttgctta tgcagggatg gaaatgaatg cggtccatgt
    79501 taacgattta gataatccaa ctaaaaaata cccaattgca attaccatcg cttctatagg
    79561 tactattgca atattcctgc tcagtacttt aggggtcgcc tttattattc ctacagataa
    79621 aattagtcta actcaaagtt tgttggttgc ctacgatatg ctgttcgaat gggctggagt
    79681 gccatggctt ggctctgtga tggcatttat gctagcaatt ggcgtactgg gtggggtggt
    79741 tacttggatt gcagggccta atacaggtgt attagcgatc gcgaaagctg gttatttacc
    79801 taagtttttc caaaaaacca atcgtcatgg aatggggcac catctaatgt ttgtacaagg
    79861 tataattgtc tcagttcttt ctgttacttt tgtgataatg ccttcagttc aagcagcttt
    79921 tcagatttta tcacagttaa cagttatgct ctatttagtt atgtatatgc tcatgttcgc
    79981 tagtgctatc tatctacgac attctcaacc tgatacaaaa cgcccatatc gagcccctgc
    80041 tttatttttc tgggcaacac ttggattctt agggtctttt ctggcttttg tattaagttt
    80101 tattccgccc gagcaaatcc caactggtag tccaaccagc tatgttcttt atttgttagt
    80161 attagttgcc ctatttttat taattccaat attaatttac gccttccgaa aatctgactg
    80221 gaaagatcca aactctgatt tcgaaccatt ttcttgggaa aaaagaacta gtaattaaat
    80281 tttccataag ctacaaaaga tgaggttaaa tatgtttagt gctgaatctc caattggtgt
    80341 aatagactca ggtgtcggtg gattgacatc ggtaaaggaa ataattaatt tgcttcctgg
    80401 tgaagatatt atctattgtg gagataatat gaatgcacct tatggcaacc gttctgccga
    80461 tgatatcatc agtttgacta aaaaaatgct gactttttta cagtcaagga atgtcaaact
    80521 agttgctgtc gcatgtaata ctatatcctc aactttggaa tccgaagaat acagcggata
    80581 tgctaagagc tttccgtttc ctatcttatc catcattgaa cctgcagtag aagatgtgat
    80641 tcgccagcaa tataaaaatg ttggtattat cgctaccgag tttactatta aaacggggtg
    80701 tcacaaagag ctaataaaaa aattgaattc aacaataaat gtatttggag aaccaagcaa
    80761 aaatcttgca atgttaattg aggaaggtaa tcttaatgct cctgcaatct tgaatgacat
    80821 caaaaaacat gttaaccatt taatttccct ccatccagta aatgaaatta ttctcggctg
    80881 tactcactat ccaattgttc agaatttttt tgaagcagtt gcaccggata taaaatttat
    80941 taatcctgca catgatcagg ctatctctat taaaaatcac ttgggacagc taaatttatt
    81001 aaactcctca aatataggaa ctttgcaaat aaatactagt ggaagtatgg agatctacaa
    81061 aacggtacta ggggaattga gcattacgaa acctcataca ttcagtatca ggcagtttta
    81121 aatttatccg gtacaccgta aaatcccgtc cttctgaact gcaccccaaa agttggacac
    81181 caactgagta aggtgcattt tatggacaaa cccagttaca tactgggtag tgcatccaat
    81241 tagtagaaca tgtgtttttc gataaacgct ccgatcactt tttcgtggat ctcaactgag
    81301 cgtgagaagc agattgtttt acgacccaac cgcttaatgc gggtgcgtag cgtcagatta
    81361 ttacgctcaa tgcgttgggt gaatattttg ccggtcagat gcttattctt cggcacctcc
    81421 cggccatagc tgccccagtc atcgctggtc agcatgccga tgttgaaggg tgtaagcagt
    81481 gccagtagct cccggcacgt ttgatcggtt cggggaccaa aagtgtaggc cagtacaccg
    81541 cctgttttgg tgttgtacgc gtacccgagc caatgttgcc gggctttact gccaacgtag
    81601 ctccattgct catcaagctc gcagataaac gccacatcag catgggcaac aggcgaagaa
    81661 gttattcgct ttggcgcgag ttttttaaag tccggatgac ggtgttacta ccaattttca
    81721 gtgtcctggc ggtatcgcga accccggcac cattaaaggc catttcagtt atcagctctt
    81781 tcatacccgg cttacgtgct tgataagtat aagtgaactg aaacacacgg tggcagtcac
    81841 ggcagcgaag tctgtcacgg cctttagggt tctgaccatg gcggtaaacc tgagctgact
    81901 gacaacgggg acaatgaata ttaacgctgg tcatgagata acctcaaaag cccgtattat
    81961 acatcagatt caactaatta gaggcatcac ccatccagca ttataaaaca tactaacaat
    82021 aactaaatta taataatcac aacttacaat caaataataa atgcataaga ttaacaatga
    82081 ttttttcggg ttatatttat ctaaaaaagc agaatattat gtaacttatt attttctaag
    82141 cgaaaattta ttgtgcggtc agactaacaa acttgcgtac cacccgaaga acatgcgaag
    82201 atttaagtat tctttctttt ttggttttta tttttcattt tctcttttct tctctttccg
    82261 gttctgttct ggttttggtt ttttcctttt tatttttatt ctttcttcag ggttatgcta
    82321 aaacaccaaa acatttttca ccacaaaagc atccaatcat cttgccagac caatcacctt
    82381 aaccggatac actgccggat ataatcccac taacacccag acaccaagca ccatacctga
    82441 taactaccgt attcaggaag gaaatgcgag tgatttcacc gtcaccggca caaccgatcc
    82501 gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca cttcgtcaat
    82561 gactgccaac gggagttgtt gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa
    82621 gtcctgcccg gccaccacca gcaggggatg cggattcgga aaacgcgcga cgaaatcgac
    82681 cagcgcgttg atgaccgtca gaaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc
    82741 cagcacgtac acgtcagttt tgggatcaag atccagctga agcagcgcct gttgccaggc
    82801 actcaccaga tccgtttcat caatcgggat cgccaccggc aaattgcgca gcggcagttg
    82861 tacgccctcc agccagattg tgctgccaga gagcgaaagg gtatgtgcac ccgcgccaat
    82921 caccgtggag cgtacggttt gcgccggaaa ctgcacattc atctcacgca ggcgcggatg
    82981 gtcatgcagc gccgttgcca gaagtgggcc aatatcggca aaacagaacg ggttggcggg
    83041 ctggtggcga taacattcgc ccacgcctcc agaaagcgta atgatttcgg gcgtaacacc
    83101 tgcgggcagc aaaccggttt gcatcaatgc ctgcgcgagc ggcgaaagcg ttccgtcaat
    83161 cacttcgaca atcagcgccg ccatccgccg ggtaacctgc accagctgcg cgccggtcag
    83221 cgaacgggcg tcagtgcctg caccgaagca ctcatccaca atcatctgcc ccggtttatg
    83281 agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg cgaccaccga cgttgagaca
    83341 ggcagtgccg ctgatttttc cggcatcgaa cagggcgtag ttcgcggtgc caccgccgat
    83401 atcgatattc agtacccgac acagccgttg ttcagaaagg gtttgcgccc cggctccgtg
    83461 accggcgatc acggattcga ggtgcggccc ggcgctggcg acgacaaaat cgcccagcga
    83521 ctgtttttcc ttcatcactt tttccatcag ctgtgcgacc aggctttcgg taaactggcc
    83581 ttccggcagc tgggcaatga tggtttcgcg gatgcgctgg ctttcggttt tgtcggctgg
    83641 cacgctggcg ggcacggacg ccgttaccgg gatcgattca tcacattcgg tgatggtaaa
    83701 acccagtaga tccgccactt cacgggcttc cggggtaata atgctggcgc gcagaaccac
    83761 ggacattgcc tgttcgccgc gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt
    83821 tttcacctgc ttcctcctgt ttggtgagag atgccagata atccaccagt tgctgcacgc
    83881 tttgcgggtc gtggctgttt agctcgaaaa tcggctcttc gaaacccgtc tcaagcaata
    83941 actttcgcgt tgcggcgacg tcagcatctg gcatgtccgt tttactgatg acggcgattt
    84001 gccgtttgct gacgccaata tccagcaacc cggcaggcaa ccgactttcc gggtcatttg
    84061 cgccatggac atagatcagc atgtctacat cctgtagcgt ggtaattaag gcgtgatacc
    84121 agcggggatg gctaaaatat tcacccggcg tatcaatatc gcccttatca ttaaattcca
    84181 cggccagtgt ttttctggcg agggtataat tcccctgcaa cgcattaaat aacgttgttt
    84241 ttcctgcccc gacgctaccc acaaacgcaa ttcgtttcat gaatgcctcg tattaacttt
    84301 tggtcatttc acaaagcgta taatttaata agcgacctaa accgctgacg gtttgtgata
    84361 acgcttcttc tactgcgccc accgagccat aaatcaccaa tgcaccgctg aagcggtcga
    84421 gaaaaccgat atgcacatca gcggctttca gcgctaaatc accggcgatc atcgccgttt
    84481 cgccgggagt tagcgtcata atgccaattg cgcccgcatc cggaacgccg attttcttcg
    84541 ccagttcctc accgggatgc gcgatgagat gcgccagtgt gacctgtttg ccgggcacaa
    84601 attcctgaat gatgcgttct ttatccatga gtcgccgcct gtgaaaaact ttcgtgtatc
    84661 gtgacaattt ttcgtcagag taaataacaa aattcggcaa gcgcgtttaa aaggtgaggt
    84721 agatcaataa gaatggagag atatttactg ttatgaataa actttcatat gcgacgcatg
    84781 aatataaata aaataatatc gagatatttt atatactgat ttttttatta ttcgcaaact
    84841 cagtaataag ctttgctaat atatttcagg aaaaccgctt accctgaata ttcagggtaa
    84901 gcgtgagagt taagaatgaa ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc
    84961 accatattga cgatacgacg caccgatgcg atcggcgtta acacgtgaac cggtttcgcc
    85021 acgcccatca gcaccggacc gacagtcaca ccttccgagc tggaaacgcg cagtaagttg
    85081 taactaatgc gggcagcttc catgttcggc atcaccagaa tattggcaga acctttcaaa
    85141 gggctgtccg gcatacggtc gttgcgaatc gcttccacca gcgctgcatc gccgtgcatt
    85201 tcaccatcaa tcatcagatc tggcgcacga tccctgacca gttccagcgc ctgacgcatt
    85261 ttgctcgacg acgggcagtc agaagaacca aagttggagt gcgacaacag agcaacgcgc
    85321 ggctcaatac caaaacgacg gacagtttct gccgccatca aggtgatctc cgccagctct
    85381 tctgcgtctg gttcgtcatt gacgtaggta tcggcaataa aggtgttacc actcggcagc
    85441 agcagcgcat tcattgcacc tgcggtgtga acgccatcgc gataaccaaa gacatttttc
    85501 accacactaa aatgttcatg ataatcgccc accgtaccgc agatcattgc atcggcttcc
    85561 ccacgctgaa ccatgatcgc gccgatcact gtcgggttac tgatcagcgc acgctgcgcc
    85621 tgttcctgag tgacaccgcg acgcttcatg atctggaagt attcggtcca gtactcttta
    85681 aagcgcggat cggattcgtt attgacgatt tcaaaatcaa cgcccgcttt gatctgcaag
    85741 cccagtttct gaatgcgcat ttcgatcacg ttcggacgac cgataaggat cggtttcgcc
    85801 agtcccagcg ttaccagttc ctgagtcgca tgcagaacgc gcgcctcttc cccttccggc
    85861 agaactacgc gcttcggcgc tttgcgagcc tgggagaaaa tcggcttcat aaacaggttg
    85921 gttttgtaaa cgaactcagt cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc
    85981 gtcgccacgc ccgactccat cgccgctttt gcgaccgcag gagcgatctt aacgatcaag
    86041 cgcggatcaa acggttttgg aatgatgtat tccggaccaa agctcagatc ctgaacgcca
    86101 tacgctgaag ccaccacttc actctgttct gcatgggcca gttctgcaat cgcgcgtacc
    86161 gccgccagtt tcatctcttc gttgatggcg gttgcgccaa cgtccagcgc gccacggaag
    86221 atgaacggga agcaaagaac gttgttcacc tggttcgggt agtcggaacg gccggtacag
    86281 atgatggcat ccggacgcac ttctttcgcc agcggcggca gaatttccgg ttccgggttc
    86341 gccagcgcca ggatcattgg tgcccgagcc attttcttca ccatttcctg ggtcagcact
    86401 ttcgggccgg aacagcccag gaaaatatcc gcaccttcaa tcacatcatc gagggtacgt
    86461 ttgccgtcat ccaccaccgc atatgcggct ttggtttccg ccatatttgg ctcacggccc
    86521 tgatagataa cgccttttga atcgcaaacc acgatgttat gtttttgcag ccccagcgct
    86581 accagcaggt tcatacaggc gattgctgcg gcacccgcgc cggaaaccac catccgcacg
    86641 tcggagatgt ttttctccac cacgcgcaag ccgttgagga tggcggcagt gctgatgatt
    86701 gccgtgccgt gctgatcgtc gtggaatacc ggaatattca tccgctcgcg cagtttctgt
    86761 tcaatataga aacattctgg cgctttaatg tcttcgaggt tgatgccgcc gaaggtcggt
    86821 tcgagcgcgg cgacgacttc aataaatttg tccgggtcga gttcatcaac ttcaatgtca
    86881 aatacatcaa tcccagcgaa tttcttaaat agaacgccct tgccttccat caccggtttg
    86941 cctgccagcg cgccaatgtt gcctaacccc agcaccgccg taccgttaga gatcaccgca
    87001 accaggttac cgcgcgcggt atatttgtag gcttttagcg ggtctttttc gatttcaaga
    87061 caaggtgcgg caacgcctgg tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc
    87121 ttggttggag aaacctggat tttccctgga actggaaatt catggaaatc aagtgcactt
    87181 tgttttaact ggtcatccat ttggttgttc ctttcacgta acgttcacaa ataaagtgtg
    87241 tgggcaacag cccctgccca caacgtggcg cacattatta ccctgccgga gtctacagac
    87301 tttgagcaag tccaaactct caccattaat ataatgtttt ggtaataatc ctataacact
    87361 gatgttacct gcttaatcca gcaataccat gcctgtctgc tatgcttttt tgatgcgttt
    87421 agcgaaattt ctcagaagtg tgaattaacg cactcatcta acactttact tttcaaggag
    87481 tatttcctat gaacgagtta gacggcatca aacagttcac cactgtcgtg gcagacagct
    87541 gcgatattga gtccattcgc cattatcatc cccaggatgc caccactaat ccttcgctgt
    87601 tactcaaggc tgccggatta tcacaatatg agcatttaat agacgatgct atcgcctggg
    87661 gtaaaaaaaa tggcaagacc caggaacaac aggtggtcgc agcgtgtgac aaactggcgg
    87721 tcaatttcgg tgctgaaatt ctcaaaatcg tacccggttg cgtgtcaaca gaagttgatg
    87781 cacgcctctc ttttgataaa gaaaagagta ttgagaaggc gcgccatctg gtggacttgt
    87841 atcagcaaca aggcgttgag aaatcacgca ttttgatcaa actggcttcg acctgggaag
    87901 gaattcgcgc ggccgaagag ctggaaaaag aaggtattaa ctgcaacctg acgctgctgt
    87961 tttcttttac ccaggcgcgg gcctgtgcgg aagcaggcgt ttttctgatt tcgccgtttg
    88021 tagggcgtat ttatgactgg tatcaggcac gcaagccgat ggacccgtat gtggtggaag
    88081 aagatccggg cgttaaatcg gtgcgcaata tctacgacta ctataagcaa caccactatg
    88141 aaaccattgt gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct
    88201 gcgatcgact gactatcgca ccgaatttgc tgaaggagct gcaggaaaaa gtttctccag
    88261 tggtacgtaa attaatcccg ccttctcaga cgttcccacg cccagcaccc atgagcgaag
    88321 cggagttccg ttgggagcac aatcaggatg cgatggcggt agaaaaactg tctgaaggca
    88381 ttcgtctgtt cgctgttgat caacgcaaac tggaagatct tcttgccgcc aaactataaa
    88441 ccagccacgg agtgttatat gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt
    88501 atggatgctg tacaaaaagc caattctggt catcccggcg cgccgatggg catggctgat
    88561 attgccgaag tgctgtggaa cgattttctt aaacataatc ctaccgaccc aacctggtat
    88621 gatcgcgacc gctttattct ttccaacggt cacgcgtcga tgctgctcta cagtttgcta
    88681 catctgaccg gttacgacct gccgctggaa gaactgaaga acttccgtca gttgcattcg
    88741 aaaaccccag gccacccgga gattggctat acgccaggcg ttgaaaccac caccggcccg
    88801 cttggacaag gtttggcgaa cgccgtcggg ctggcgatag cagagcgtac actggcggcg
    88861 cagtttaacc agccagacca tgagatcgtc gatcacttca cctatgtgtt tatgggcgac
    88921 ggctgcctga tggaaggtat ttcccacgaa gtctgttcgc tggcaggcac gctgggacta
    88981 ggcaagctga ttggttttta cgatcacaac ggtatttcca tcgacggtga aacagaaggc
    89041 tggtttaccg acgatacggc aaaacgtttt gaagcctatc actggcatgt gatccatgaa
    89101 atcgacggtc acgatccgca ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa
    89161 gataagccgt cgctgattat ctgccgtacg gtgattggct ttggttcgcc gaataaagca
    89221 ggtaaggaag aggcgcacgg cgcaccactg ggggaagaag aagtggcgct ggcacggcaa
    89281 aaactgggct ggcaccatcc gccatttgag atccctaaag agatttatca cgcctgggat
    89341 gcccgtgaaa aaggcgaaaa agcgcagcag agctggaatg agaagtttgc cgcctataaa
    89401 aaggctcatc cgcaactggc agaagagttt ccccgacgga tgagcggtgg tttaccgaag
    89461 gactgggaga aaacgactca gaaatatatc aatgagttac aggcaaatcc ggcgaaaatc
    89521 gctacccgta aggcttcgca aaatacgctt aacgcttacg ggccgatgct gcctgagttg
    89581 ctcggcggtt cggcggatct ggctcccagc aacctgacca tctggaaagg ttctgtttcg
    89641 ctgaaggaag atccggcggg caactacatt cactacgggg tgcgtgaatt tggcatgacc
    89701 gctatcgcca acggcatcgc gcaccacggc ggctttgtgc cgtataccgc gacgttcctg
    89761 atgtttgttg aatacgcccg taacgccgcg cggatggcgg cactgatgaa agcgcggcag
    89821 attatggttt atacccacga ctcaattggc ctgggcgaag atggtccgac gcaccaggct
    89881 gttgagcaac tggccagcct gcgcttaacg ccaaatttca gcacctggcg accgtgcgat
    89941 cagatggaag cggcggtggg ctggaagctg gcggttgagc gccacaacgg accgacggca
    90001 ctgatcctct caaggcagaa tctggcccag gtggaacgta cgccggatca ggttaaagag
    90061 attgctcgtg gcggttatgt gctgaaagac agcggcggta agccagatat tattctgatt
    90121 gccaccggtt cagagatgga aattaccctg caagcggcag agaaattagc aggagaaggt
    90181 cgcaatgtac gcgtagtttc cctgccctcg accgatattt tcgacgccca ggatgaggaa
    90241 tatcgggagt cggtgttgcc ttctaacgtt gcggctcgcg tggcggtgga agcaggtatt
    90301 gccgattact ggtacaagta tgttggtctg aaaggggcaa ttgtcgggat gacgggttac
    90361 ggggaatctg ctccggcgga taagctgttc ccgttctttg gctttaccgc cgagaatatt
    90421 gtggcaaaag cgcataaggt gctgggagtg aaaggtgcct gatggtgatc gccggatgct
    90481 gtatgttccc ctcaccctaa ccctctcccc agaagggcga ggggacggtc cgtgcgcttt
    90541 ttagggccgc atctgcgatt ttgtttaacg ggtgatccac aacgtgggcc tggcgtctgg
    90601 cccatgccag ttatcgcagg taggttctgc ggcgtaacgc accaggcgaa aacgctgacc
    90661 gtcaaagcgc cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt tcgctaaggt
    90721 caccagttca cgcgatttct catcaaatgt tgcgttcatc agttccagtt cattcgtctc
    90781 ctggccgttg ttgaacggca aacgcaaccg aaccgggcgc gaggctagcg gctttttacg
    90841 cgacacaatc catgccaaat caatggtgtt ataggcccct gcctcacagc taatcatcat
    90901 cagtgcttta tcatcggtca gcgcagtgac attcacctca cgacgtaacg gatcaagcga
    90961 gcagcgcaga ccattcatcc gccagtttcc ataatccagc aaatcgttac gttcttcgag
    91021 tgagagtggc gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag gcgcgggcgg
    91081 tacgctgaga ggcggttcgt cccctttctt gatccacgcg gtttcactgc caacgcgctt
    91141 ttgctgagca tcaataaaca acaacgccgc ttttaagccg ctcagagaaa tggtctgatt
    91201 gccgtcgcgt aaggtgatcg ccttcccttc ctgaattatc tgcaaaaacg cggtgatggt
    91261 tgccgtatca tcggttacta ataaccatgg tgaaatccgc cacttgtcac cacttaatgc
    91321 caacggctca ccatctaata gcagccgtgg cgctatctcc ccttcagacg catccggcga
    91381 cttcaaccca ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccag cgctgcggct
    91441 cagggtcatc accagtccat tatgatcgcc cgtattgcgc gccacacaaa aattttgatt
    91501 attacaggtg acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc acaccaaaga
    91561 cgtcggcaac aaagcgaaaa aaaagagaag gaaaatgcga tagcgcatgg acggtacgac
    91621 cccagaagtt caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa
    91681 tcggatttgt cggattgagc caataataaa caacgaaata ttattgctat ttttcagtcc
    91741 attaaatgtg acatttgcaa atagttaagc aataacaccg tcttaccgtc acgtatctcg
    91801 ccggttttga tcatctccag cgcctggctg aacggcagct cgagcacttc aatatcttca
    91861 tcttcaacac cgccaccggc gttagcgcgc tgattgtcac tgtattcggc gataaaaaag
    91921 tggattagct cagtcacacc gccaggcgac atatacagtt caaataattt gcgcacttcg
    91981 cccacttcat agcccgtctc ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg
    92041 ttatccagca gcccggcgca ggtttcaatc agctgcccgc tttcattgcc attaacccag
    92101 gtagcgacac ggaactgacg aatcagaacc acgctctttt tcttcgcgtt gtacaggagg
    92161 atcgtcgcac cattaccgcg atcgtaaact tcacgtttat ggcggataac ttcgccatct
    92221 ttgcgcgtga gatcgtaagt aatgttgtgc agggtgaaat agttatcgga gagaattttg
    92281 tctttaatga gggtgatttg ttgcgtcata ccgactccac agcgcgaaat gaacaattat
    92341 attacgctgt gaagtcgggt ttgtctgcgc aggctatcag tgagatttga ctgattttac
    92401 ccccagccag tcaataatcc cctgtgccgc atgacgtcct tctgccattg cggtaaccac
    92461 cagatccgca ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg tggtctggta
    92521 acggtactgg ctttccacat ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg
    92581 cgactccagc cacggcatcc cgtgtggatt gaagccaaac gccataatca ccgcatcggc
    92641 tggcatgaca aattcactgc cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg
    92701 ctcgccaaga cgcgtgcgca ggaaacgaat tccgcaaacg tgaccttgct cattcagctc
    92761 aagcgccacc ggctggacgt taaattcgaa gttggccccc tcttcgcggg cgttcttcac
    92821 ctctttcttc gagcctggca tgttagcttc atcacgacga taagcgcagg tgacgttact
    92881 cgcgccgtgg cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac
    92941 cacgacgtta agtccggcgg tattgataaa cggctcttcc ggtagctctt cgagccccat
    93001 cacctgttta gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg gcgcatcttc
    93061 attgggtaaa cccgctttca tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta
    93121 ttgttccaaa agcaaatcca aagagacatc tttacccact tcacaattga gttcgaagtg
    93181 aatccccatc gcgctgaaga tttcccgacg gcgtgccagc agggatttat ccagtttgaa
    93241 agaaggaatg ccgaaagtga gcaagccacc gatttctgga tggcgatcgt acaccgtcac
    93301 cgccacgcca ttgcgggtca gaacatccgc acaggccagc cctgccggac ctgccccgat
    93361 aatcgccacc cgcttgtcca ctttggtgac atgacttaag tcaggacgcc aacctttcgc
    93421 caacgcctga tctgaaatat agcgttcaat gttgccgata gttaccgcgc cgtgctcatc
    93481 gcgaatagta caggcacctt cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc
    93541 cggcagggtg ttggtctggt gagaaagctc gacggcggcg tcgatgtttc cggctttcac
    93601 cagttcaatc cactgcggaa tatggttatg cagcgggcag gtccattcac aaacgctgtg
    93661 ctcgccgaac ttaaggcagc gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag
    93721 ataaatttca tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg
    93781 cgcgggcgtt gcctgcattt gttcgacttt actcattacc ggcatttctt gtgcagcggt
    93841 actggcatgc cacggttgat gctcctgacg tgcggtgcgc aagcggcggg atttcgccat
    93901 gccggagagt gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat tctcgacaca
    93961 ggcaggaccg ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg cttttacttt
    94021 tcctgccgcg acgggtgtca ggacgatttg catcgtacca aacggacagg ccaccacgca
    94081 ggatttacag ccaatacact tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat
    94141 tgcgccatta gggcagctac gggcgcaggg cgcatcttca caatggtgac aggtcactgc
    94201 actacgttgc tgctgatgtt tgataaccgt aattcgggga tgaaaatggt gttggctcag
    94261 gacatgttgc tcatcattgt gagccatgac acaggcgatt tcacaagcat gacaacccag
    94321 acattgctga ctgttggcca taataaaacg attcataacg accttctttt ttggttgtaa
    94381 aaaccttatt ctttatatga gtgttgttat tacccgactt acaggggaac cggcaatgtt
    94441 catgtgccca gaataagtaa ctatttcgct ataaactgtg gcagatcaaa taatcccatc
    94501 actgactaaa ttgcgtttca gcgaactgga acattaatga ttttttgtgg agaagacgcg
    94561 tgtgattgtt aaacgacccg tctcggccag tctggcccgg gccttttttt acattgtgct
    94621 gctgtcgatt ctttccacgg gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc
    94681 tgaggctatc aatattgccg gatcgctgcg tatgcagagt taccgcctgg gctacgactt
    94741 gcaaagtggc agtccacaac tcaatgcaca tcgccagttg tttcagcagg cactgcattc
    94801 accggtatta accaacctca acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc
    94861 gcatctgaat gccaactggc tggagatgaa taatcggctc agcaagggcg atttgccgtg
    94921 gtatcaggcc aatattaata attatgttaa tcagatagac ctgttcgtgc tggctttaca
    94981 gcactacgct gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg gcggtatcgg
    95041 tattttcacg ctggtctttt ttactttgcg ccgcatacgc catcaggtgg ttgccccgct
    95101 gaatcagctg gttaccgcca gtcagcgtat tgaacacggg cagttcgact cgccgccgct
    95161 ggataccagt ctgccgaatg agcttggtct gcttgcaaaa acctttaacc aaatgtcgag
    95221 cgagctgcat aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct
    95281 ccacgagacc aagcgtcgtc tggaggtgtt gtatcagtgc agtgttccca ggcgctgaac
    95341 accagccaga ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa
    95401 gcagctgaat atctggagct gaacgttggg gataactggc ggattagcga agggaagcca
    95461 aaccctgagc tgccaatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa
    95521 ctgcactggc aaaatagtca cgtttcatca tcagaaccgc tgcttaacag tgtttcatcg
    95581 atgctgggac gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg
    95641 atggaagaac gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca ggtgctttct
    95701 tacttacgta tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca
    95761 caaagtatca tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag
    95821 ctgttgacta ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa
    95881 atgctggata cgttacaaaa tcaaaccagc gctaaactga ccctcgactg ccgtctgcca
    95941 accctggcac tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg
    96001 ctgaatgcga tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg
    96061 gacggcaatc acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa
    96121 cccgaaggtc attatggact gaatatcatg cgcgaacgcg cggaacggct gggcgggacg
    96181 ctgacttttt cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctccgca
    96241 gagggtgagg ggaaggggaa cacgccggct gaggtgtaaa taattaccct cacaccggcc
    96301 ctctcccttt aaaggcgaga gggaaaaacg tgcccatatt gacctgtaag cgagtgtgtc
    96361 agcaatctca tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg
    96421 ccgctacagt gaagcaagtc aagcctacaa caatacgcag aaacacgagg tcctctttta
    96481 atggcgaatt tctttattga tcgccccatt tttgcctggg tgctgacaat cctgttgtgt
    96541 ctgacaggta ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca
    96601 ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc
    96661 gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctgatgta tatgtcatct
    96721 cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg
    96781 gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag
    96841 gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt
    96901 gccttcgtct ctaccgatgg ctcgatggat aaacaggata ttgctgatta tgttgccagt
    96961 aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg
    97021 caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct
    97081 aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt
    97141 ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg
    97201 caaacaccag aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta
    97261 aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc
    97321 ttcaatggta agccagcctc cgggttgggg gtaaaactgg cctccagcgc aaacgaaatg
    97381 gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcacgga
    97441 ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg
    97501 gtgaaaacgc tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa
    97561 aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc
    97621 tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg
    97681 gcgatcggtc tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga acgtattatg
    97741 agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg
    97801 gcactggtcg ggattgcgat ggtgctttcg gcggtatttg taccaatggc cttcttcggc
    97861 ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg
    97921 tcagtactgg tagcgatgct cctcactccg gctctgtgtg ccacgctact taagccactg
    97981 aaaaaaggtg agcatcacgg gcaaaaaggt ttttttgcct ggtttaacca gatgtttaac
    98041 cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg
    98101 attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg
    98161 tcgttcttac cgctggaaga ccggggcatg tttactacct cggtacaatt gcccagcggt
    98221 tcaacccaac aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat
    98281 gaaaaagaca acatcatgtc ggtgtttgcc accgttggtt ctggccctgg gggtaacggg
    98341 caaaacgtgg cgagaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc
    98401 ggcacctcgt ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat taaagaagct
    98461 cgcgttatcg ccagcagccc accagcaatt agcggtcttg gtagttctgc aggttttgat
    98521 atggagttgc aggaccacgc tggagcgggt cacgatgcgc tgatggcagc acgtaatcag
    98581 ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgat
    98641 gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgat
    98701 atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt
    98761 atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg
    98821 ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct
    98881 gctttcgcga cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta taacggttat
    98941 tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat
    99001 attatggaat cgttagtgaa gcagctgcca aacggctttg gtctggagtg gacggcgatg
    99061 tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg
    99121 gtggtattcc tgtgtctggc ggcattgtat gaaagctggt cggtaccgtt ctcggtaatg
    99181 ctggtcgtgc cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa
    99241 aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc
    99301 atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccatgacct gtttgaagcg
    99361 acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc
    99421 ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg
    99481 ggtactggcg taatgggcgg gatgatttca gctactattc tggctattta cttcgtgccg
    99541 ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata
    99601 aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc
    99661 gggttcttgc tgtttatact ttgtctcagg aattacttac gaagcataac ttcgataaag
    99721 tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt
    99781 ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc
    99841 gggggacaaa ccttatgtaa atactatggt cctgcggtga taatttgtat gtgatacaca
    99901 gcaacatttc gagatattca tacggcatct aatactgatt taattctggt taaaatacag
    99961 acagataaca agatgaatat tcttaatgtt tacgttaaaa atgtttaata ttatttaata
   100021 gttgttaatt tgaatacttc gataatgtta tattttctga taatcatttg caggcaaaat
   100081 gttttcaccc ttaaatgagt atttattctc ataaatcgaa aaaggattca ttatggttac
   100141 actttacggc atcaaaaatt gtgacaccat taaaaaggct cgccgttggc tggaagccaa
   100201 taacatcgac tatcgttttc atgattaccg cgtcgatggg ctggacagcg aattattgaa
   100261 cggttttatc aacgaattag gctgggaagc gttactcaac acccgtggta caacctggcg
   100321 taaactggac gaaactaccc gcaataaaat caccgatgcg gcctctgcgg cggcattaat
   100381 gactgaaatg cctgcaatta tcaaacgtcc attgctctgc gcgcctggta agcctatgct
   100441 gctgggtttc agtgattcca gttatcagca atttttccat gaggtgtagt ctatgtcgtg
   100501 cccggttatt gagctgacac aacagcttat tcgccgccct tccctgagtc ctgatgacgc
   100561 aggatgtcag gctttgttga ttgaacgttt gcaggcgatc ggttttaccg ttgaacgcat
   100621 ggattttgcc gatacgcaga atttttgggc atggcgtggg caaggtgaaa cgttagcctt
   100681 tgccgggcat accgacgtgg tgccgcctgg cgacgccgat cgttggatca atccgccgtt
   100741 tgaacccacc attcgtgacg gcatgttatt cgggcgcggt gcggcagata tgaaaggctc
   100801 gctggcggcg atggtggtag cagcagaacg ttttgtcgca caacatccca accatacggg
   100861 gcgactggca tttctgatca cttctgatga agaagccagt gcccacaacg gtacggtaaa
   100921 agtcgtcgag gcgttaatgg cacgtaatga gcgtctcgat tactgcctgg tcggcgaacc
   100981 gtcgagtatc gaagtggtag gtgatgtggt gaaaaatggt cgtcgcggat cgttaacctg
   101041 caacctcacc attcatggcg ttcaggggca tgttgcctac ccacatctgg ctgacaatcc
   101101 ggtacatcgc gcagcacctt tccttaatga attagtggct attgagtggg atcagggcaa
   101161 tgaattcttc ccggcgacca gtatgcagat tgccaatatt caggcgggaa cgggcagtaa
   101221 caatgttatt ccgggtgaac tgtttgtgca gtttaacttc cgcttcagca ccgaactgac
   101281 tgatgagatg atcaaagcgc aggtacttgc cctgcttgaa aaacatcaac tgcgctatac
   101341 ggtggattgg tggctttccg ggcagccatt tttgaccgcg cgcggtaaac tggtggatgc
   101401 ggtcgttaac gcggttgagc actataatga aattaaaccg cagctactga ccacaggcgg
   101461 aacgtccgac gggcgcttta ttgctcgcat gggggcgcag gtggtggaac tcgggccggt
   101521 caatgccact attcataaaa ttaatgaatg tgtgaacgct gccgacctgc agctacttgc
   101581 ccgtatgtat caacgtatca tggaacagct cgtcgcgtga tgagtggttc tgcaagagga
   101641 aataagcatg gactggctgg ctaaatattg gtggattctg gtgattgtct ttttggtagg
   101701 cgtcctgctg aacgtgatta aagatctcaa gcgcgtcgac cataagaaat ttctcgccaa
   101761 caagccggag cttcccccgc atcgtgattt caacgataag tgggacgatg acgacgactg
   101821 gccgaaaaag gatcaaccga agaagtaagc tgagtgctca accgtaggcc ggataaggcg
   101881 ttaacgccgc atccggcaat ggtgaacgat ccctgatgcg acgctcacgc gtcttatcag
   101941 gccaggtaat ctgtctttta catcatttca atcacatcat catcgccagg cttaccgcca
   102001 cttagcgctt ggtcgaaata atgtttcgga atggtataac gcaagtgatc cagggcaaat
   102061 tgcatactgc gattgtcgat cgcatggcct aaatcctcca cgatatccag cgtcacgtca
   102121 ccaccggcac tgattaacgc ttcctgcgcc gcaactgcat gcgccagatc gataaccgga
   102181 tcttcaccac catgaatcaa gtgaattgtc gtggctgtag acgcggtctc cggcaggctc
   102241 gcatagcgtc cgttaaaggc aatgacgcgc gaagcaaggc ccggttcggc tttaatgctc
   102301 tccagcgcca taatcgcgcc ttgtgagaaa ccaattagcg ccgtggcgtt agcccctact
   102361 ccgctctgtt tctgccagta gcgtaccgtt tcaataaacg tcggcatgat ggcatccaca
   102421 cgcgcctggc gattatcttc cgtaataccc tgcactgaaa accactgacg ccccgccgga
   102481 ttaccgctcg gctccgcgcc accgacactc accaccagcg catcaggaaa cagcggtgca
   102541 aaccagctgc ctatttcccc cattgccacc gggttatccc cgacaccgtg gaaaagcagt
   102601 aacaactgtt gtgcaggttt atccgggctt tgaacaacaa aatggtcatg tttcatggcg
   102661 aactccttaa ctgatgtcat taattttacg ccgctgactc acaatgacca tgggagaaaa
   102721 ttgaaggagt cagtgaaaaa attgccattg cgttatgcga tcgcgcagac gctcagtgcg
   102781 aacctcattt agtgcgaaca gcgcctgcgc ggcctcttcc cgctggcgaa ccagtaacat
   102841 cttgcggcct gaaagtttaa gtgtggtaca taactgcgcg tcactggcgt ttttctgtaa
   102901 acgcccacgc agcgccggta atgccagttc actggtttgt agcagacgca ataagcaacc
   102961 taacgatgtt aatagcggac gatgagcgaa agcaaaaccg gccagttcaa gccagtcgtc
   103021 gtcagaaagg atggcatcgt ttagtggatc aacaggaagc atttcgccat tccactgcgc
   103081 gagagcttgc gcatcgcgac gtaaacggta atgctcacgt tcagccagct gtttacccgc
   103141 atcactcatc ggtaacagcg ccatcgccgt atagcaaccg ctgctggctt cccgatgatt
   103201 acccatccgc accagcacaa aaccgcagcg ttgccagaaa cgccataact ccccagtgta
   103261 accaaaactc accgaaagat agtcgaggtc atgcgtatat tgcaaagctc cagcaataag
   103321 ttgtcgccct gtgccttccc gctgacgagc cggatgaact gctatccggc taacccgcct
   103381 tccgcgcaat gtcgccgcca gtggattgct gccgtgcgcc gccagcgact gggccaccag
   103441 attaccccgc gggcgacgaa aacctgccca taccgcctga ctgagttctt gagataatcc
   103501 gccctcatcc accagccaca acgccccggc aatctcgttt tcgccagccg cctgtaaaaa
   103561 atgttgccct ggtgcatcca tcatccggcg taaatccagc ggcgaggtcc ggtagtgcgc
   103621 accagacaat aactgataaa cctttaacgg cgtttctggc tcgcttcgcc ataacgtttg
   103681 ttcaaatgcg gaaatgacga tattgccttg tggtgtatgg gtgaagtttt catcgttaaa
   103741 aaccagtgcc tcactaacca ttttttccag cgggcatccc tgtgcccagc ggatcggctg
   103801 ttgcagttca aaacggtgta aatgcggaaa gcgagcgcaa aatttcagca aaaaaccacg
   103861 tccggtacct tcgtagccct gcaccgtagt ggttaacaac gttcgaggaa aacgcgatac
   103921 cagttgatgc aacaacggcg caggtatggc tgcggcttcg tcgaccacca gccagtcggc
   103981 ttgctcatcg ctggctaaca aggcatccgg tgcaataaag cgaaacttct cgcccgcaaa
   104041 ttgtgccagt acatccgttg ccgcttttgc gggcgcggtg acaatcgcac tgcccgcaat
   104101 acgagaaatg agttgccctg ccaacgccga cttaccgcgc ccacgcgcag ccgttaccgc
   104161 tgccacgccc ggcggcatgg tcagtagctg ctgtaagagt tgctgttgtt ctggttgtgg
   104221 tgcgccggtc gcggggtgcc agtcagtacg gggagtaaaa tgcgccaacg agaacggctg
   104281 gttttgccgc cagaggatag cgtcgttatc cgccgtaagt acgcgtttga gatgctggac
   104341 aaaatgcggc gtcgcaatag ggtcagggca atcactccag cgcagcgagt cggcatcagg
   104401 ttggttttcc cactcttccc atacagggag taacaaaacc agccagcttc ccgctttcaa
   104461 cgttccgcta agtgctgcaa aggcagcggc atcaaagccg tggcgggcgt cgaataccgc
   104521 atgccggaac tcgcgcccaa gtaaagtttg tagcgccgag ggtgaacagt ggttttcagc
   104581 atctagctgc ggcgaaatcc acagccagtc gccaggtaag gcatcacgca acttaagagc
   104641 atgatcaaaa caccaaccct cttccccgct caacaccagc aagcggcaga tcccttcacg
   104701 tttcatttgc gctgttaatg tgtaaagcgc agtcagttca gccatccctg ccccggaaat
   104761 taaatgcttt taccaaaagt attgcattgt gccggatcgc cgctgtcgaa accacgttta
   104821 aaccagctgt agcgttgctg agaagtgcca tgtgtgaaac tgtctggtac tactcgcccc
   104881 tgactttgct gttgtaaacg gtcatcgccg atggcctgcg ccgcattcag cgcctcttcc
   104941 agatcgccag tttccagaac gccttgctgc tgcatactat gcccccagac accggcaaaa
   105001 cagtcggcct ggagttccat acgcacagat aagcggttta cttccgcctg cgtcgcgttt
   105061 tgttgcagtt gacgaacttt cggctcgatg cctaacagtt tctgcacatg atgaccgact
   105121 tcatgggcga taacgtaccc ctgggcaaaa tcgccatccg cgccaagttt gtctttcatg
   105181 tcatcataga aggagagatc gatataaact gtgccatccg ccgggcaata gaacggcccc
   105241 ataatggact ggcccgcccc gcagccggtg cgcgtcattc cacggtacat gaccagcttc
   105301 ggttgctgat aggtcttacc catcttttcg aactgttgtc cccaggtgtc ttccgtggtt
   105361 gcctgaatca ccgaggtgaa ttttgcggct tcgtcttcat tagggctaat tgaccgcgtt
   105421 gattgttgtt gagaaaccgg ctgcccggtc atcaacccgg ttaaatcaac gccatagtag
   105481 cctgcaacca gcacgactat cagtaaaatc agcccgcctt taccgctcgg cagacggaaa
   105541 ccgggaccgc ccattgacgg accgccagag ctgttgcgcc tgtcttcaac attgtcactt
   105601 tcacgtcgcc cttgccaacg catagatacc tcaacaatat attcattata gaaatgatcg
   105661 taggtggttc agaggaagat taccacagga aagcgggatg aaatggcgga ggaatgagcg
   105721 ttcaatgcat atattccgag agcggttgtc ttgccgtgcc agctgcacgg caagatgatg
   105781 aacagaaaaa tcagtccagc tgtacaccca ggcggcgggc gacggcttca taggcttcga
   105841 tcaggccacc gaggctctgg cggaaacggt ctttgtccat tttctccagt gtttctttgt
   105901 cccacaggcg gctgccgtcc ggggagaact catcgcccag taccacttca cctttgtaca
   105961 gaccaaactc cagcttgaag tcgaccagaa tcagaccagc atcatcgaac agttttttca
   106021 gcacgtcgtt cgctttgtag gtcagctctt tcatacgcgc caggttctct ttgctcaccc
   106081 agccaaaggt ttcgcagtaa gattcgttga ccatcggatc gtgcatggca tcgtttttca
   106141 ggaacagatc gaacagcggc gggttcagct caataccttc ttcgattcca agacgtttca
   106201 ctagagagcc agcagcacgg ttacgcacga cacactcaac cggcaccata tccagctttt
   106261 tcaccagaca ttcggtatcg gagagcagac gctccatttg agtcgggata cccgcttcag
   106321 ccagtttgct cataatgaag tagttgaact tgttgttcac catacctttg cgatcaaact
   106381 gctcaatgcg cgcgccatcc cctgctgacg tatcattgcg gaattcgagc accaacaggt
   106441 ccgggttttc cgtgctgtat acggttttcg ctttaccacg atacaactca gcttgctttt
   106501 gcatctttat cactcctggg tgtgaattaa cgttttaaaa tcttttgctg tctggtgtgc
   106561 cggatgtttt gtcggatgcg gcgtgaacgc cttatccgaa ctacgtccga caaacaaatt
   106621 tcgtgcgaat taccgctaaa atcgcgtttt cctgccgacg cacacgtttg cgtatcatat
   106681 cagaaaaaag ggccggatga ttccagccct gtatttttac ttgctaaacg cagcctggaa
   106741 gacagctacc agcgcgtcgt tctgactctg agtcagagta tgacctttcg gatcgatgaa
   106801 ctgtaggctg ctgcggttat ctaaatcgcc aacctgcagt ttatagtcac cggatgccag
   106861 gcctggatcg ctcgcgccca gttcctgcca gtcgctgtca gacagcggct tataagttac
   106921 ggccatgttg ccctgcgaac gggtgctgtc ggtcactttc atgcccactt tttccagcgc
   106981 cgctggcaga cgttgccaaa ccacattgaa cggcccgcgt acgaccagca ttggtaaacc
   107041 ggtgtcatca gctgcacttt gtacgtccat agtggtggag gcacgatttt gcgcagcgtt
   107101 cgcggcgtca gtggcagatt tatccagacc ggcggaaata acgttcatca tctccgtgct
   107161 gtaacgctgc atggaagccg cgtctgcaac cggtttgccc gcctgttcca ggttcagcag
   107221 tttaaccgta accgcctgct gataaccctg cggcttaaca gagatttgat aacgaccacg
   107281 atactgctcg tcttcgtcca gacggttcca ttgtacccaa tcggtggtca gtgtctgacc
   107341 agcatcatca cgttgggtga tggtgtagtt tttcgcctgc agcacgctaa ccacctgcgg
   107401 ccacagagta ttgccacgac cattttccac cagcaatgaa gcggtatcgc ccgtgaactg
   107461 ggtacgcgcg ccagaaacca gtgccaacgg ctgggctggt ggacgaatgt ccagcgcctt
   107521 accgacagca ccactaccgt tggtcaccgg gattgcataa tcaccggagg tcaccggcaa
   107581 aatcattcca gccggggcat gaagctccgc aagcggtgcc gcttccaggt aggcttcatc
   107641 accactgacc tgacgcttat agcgtgagtc agaactacag gcagcgagta ataaaacaag
   107701 cgaaacaccc gcaacctttg ccaggcgcga cttttgaaca gagtaagcca tcaaatctcc
   107761 ctaaacttta cagcaaaccg gcatgcttaa gcgccgctct gaccgtctca cgaccactgt
   107821 cggtgattgg tgtcattggc aggcgcagcg tatcggtcgc cacaagaccc agttccttac
   107881 atgcccattt caccgggatt ggattgggtt cgacaaatag tttgttgtgt aatggcatca
   107941 gacgctgatt aataacgcgt gcctcggcaa aatgcccttc tgctgccagt ttgcacatct
   108001 gggccatatc acgcgctgcg acgttagccg taacggaaat aactccatga ccgcccaatt
   108061 gcatgaagtc cagcgcgctc gcatcatcgc cgctcagcag aacaaaatca tctgaaacca
   108121 gctctttgat ctggtttaca cgcgttaagt tccctgttgc ctctttgatt ccgataatat
   108181 tttttacttt cgccagacgg cccaccgttt ccgggagcag atcgcagcca gtacgggacg
   108241 gcacattata cagaatttgc ggcaggtcag tatgctcagc gatggctttg aaatgctgat
   108301 acaaaccttc ttgcgacgga cgattgtagt aaggggttac cgtcaggcag ccgacgatac
   108361 cactgtcatt gaagcgctgc gtcaggctaa tggcttccgc agtagcgtta gcgccggtcc
   108421 cggcaattac cggaatgcgc ccatcagcca gatccagcgt catcatcacc acatcagcat
   108481 gttcgtcatg atttaaggta gcggactcgc cagtggtgcc aacagaaacg atcgccgaag
   108541 taccgctggc gacatgataa tcaatcagtt ttttcaagct agcccgacag acattacctt
   108601 tttcatccat cggagtaaca atcgcgacaa tacttcccgt gaacatgggc catcctctgt
   108661 gcaaacaagt gtctcaatgg tacgtttggt atggcattaa aagcaagcag acagaaccgt
   108721 tctgattgtt gtatgcatga tttttttatg ctttccttaa gaacaactca ccccttaaag
   108781 gaataaccag tttgacactg tcatcgcaac attatctggt gatcactgcg ttgggtgccg
   108841 atcgccctgg aattgtgaac accatcaccc gtcatgtcag tagttgcggc tgtaatattg
   108901 aagacagtcg cctggcgatg ctgggagaag agttcacgtt tatcatgctg ctttccggtt
   108961 catggaatgc catcactctg attgaatcaa cgttaccgtt gaaaggtgcc gaactggatc
   109021 ttttaatcgt gatgaagcgc acgacggcgc gtccgcgtcc gccaatgcca gcatctgtct
   109081 gggttcaggt cgatgttgca gactccccgc atttaattga acgcttcaca gcacttttcg
   109141 acgcgcatca tatgaacatt gcggagctgg tgtcgcgcac acaacctgct gaaaatgaac
   109201 gggctgcgca gttgcatatt cagataaccg cccacagccc cgcatctgcg gacgcagcaa
   109261 atattgagca agcgttcaaa gccctatgta cagaactcaa tgcacaaggc agtattaacg
   109321 tcgtcaatta ttcccaacat gatgaacagg atggagttaa gtaatgaatc cactgaaagc
   109381 cggtgatatc gcaccgaaat ttagcttgcc ggatcaagac ggagaacaag ttaatttgac
   109441 cgacttccag ggacagcgtg ttctggttta tttctacccg aaagccatga cccccggctg
   109501 taccgtacag gcctgcggct tacgcgataa catggatgag ttgaaaaaag cgggcgttga
   109561 tgtgctgggt atcagcaccg ataaacccga aaaactctcc cgttttgcgg aaaaagagct
   109621 gcttaacttt acgctcctgt ctgatgagga ccaccaggtg tgcgaacaat tcggcgtctg
   109681 gggtgaaaag tccttcatgg gcaaaaccta cgatggtatt catcgcatca gcttcctgat
   109741 tgacgctgat ggcaaaatcg aacatgtctt tgacgatttc aaaaccagca atcaccacga
   109801 cgttgtgctg aactggctga aagaacacgc ctgattactt tgctccattc cgtgctggct
   109861 gcgcttgcgg ccagcatacc tcacttctcg tgatcaagat cacattctcg ctttcccctg
   109921 cgacacgggt gtcgaatcca ttttttgctg aacgttaatg accatcattt ttgtaccgtt
   109981 cagaatccag ttaatacata acttattgaa tagattgagt taatcagaat ggcatccttt
   110041 atacaatatg aaatgcaatg tttcatatca ttttcaagga gccgacatga accgctttgt
   110101 ggtggccgaa ccactgtggt gtacaggatg taatacctgt ctcgctgcct gttcggacgt
   110161 gcataaaacg caaggtttac agcaacaccc gcgcctggcc ctggcgaaga cgtcaacaat
   110221 cactgcccct gtcgtgtgtc atcactgtga ggaagcccct tgcctgcagg tctgcccggt
   110281 caatgccatc tctcagaggg atgatgcgat ccaactcaac gaaagcctct gtattggctg
   110341 caagctttgc gccgtggtct gcccatttgg cgcaatcagc gcttcaggaa gccgtccggt
   110401 gaatgcccat gcgcaatatg tttttcaggc tgaaggctca ctcaaagacg gcgaagaaaa
   110461 cgtgccgccc caacatgcgt tgctgcgctg ggaaccgggt gtccagaccg tcgcggtgaa
   110521 atgcgacctg tgtgatttct tgccagaagg tccggcctgc gttcgcgctt gcccgaatca
   110581 ggcgttacgg ctgatcaccg acgatagcct gcaacgtcag atgaaagaaa aacagcgcct
   110641 tgccgcaagc tggtttgcca atggcgggga gtctcccctt tccctcactc aggagcaaca
   110701 ctaatggatg ccctgcaatt attaacctgg tcgctgatcc tctatctgtt tgccagtctg
   110761 gcttcgctgt ttttactcgg tctggacaga ctggctatta agctttccgg catcacatcg
   110821 ctggtgggcg gcgtgattgg catcatcagc ggaattacgc aattacatgc tggtgtaact
   110881 ttagtcgccc attttgccac cccttttgac tttgccgatt taaccctgcg aatggatagc
   110941 ctctcggcat ttatggtgct agttatctcc ttgctggtgg tggtttgttc gctctattca
   111001 ttgacttata tgcgcgaata cgagggcaaa ggcgcggcgg cgatgggctt ctttatgaat
   111061 cttttcatcg catcgatggt tgccctgctg gtgatggaca acgctttttg gttcatcgtg
   111121 ctgtttgaaa tgatgtcgct gtcttcctgg tttctggtca ttgccaggca ggataaaacg
   111181 tcgatcaacg ctggcatgct ctactttttt atcgcccacg ccggatcggt gctgattatg
   111241 atcgccttct tgctgatggg gcgcgaaagc ggcagcctcg attttgccag tttcgcacgc
   111301 tttcactttc tccggggctg gcgtcggcgg tgtttctgct ggcctttttc ggttttggcg
   111361 cgaaagccgg gatgatgccg ttgcacagct ggttgccgcg cgctcaccct gccgcaccat
   111421 cgcacgcttc ggcgttgatg tctggcgtaa tggtcaaaat cggtattttc ggcatcctga
   111481 aagtagcgat ggatctgctg gcgcaaacgg gtttgcctct gtggtggggc attctggtga
   111541 tggcgatcgg cgcaatctcc gcgctcctgg gcgtgctata tgcgctggcg gaacaggata
   111601 tcaaacggct gctggcctgg agtaccgtcg aaaacgtcgg cattattttg ctggcagtcg
   111661 gtgtggcgat ggacggtctg tcactgcacg acccgctgct caccgtggtt ggactgctcg
   111721 gcgcactgtt tcatctgctc aaccatgcgc tgttcaaagg gctgctattt ctcggcgcgg
   111781 acgcgattat ttcgcgtttg catacccacg acatggaaaa aatgggggca ctggcgaaac
   111841 ggatgccgtg gacagccgca gcatgcctga ttggttgcct cgcgatatca gccattcctc
   111901 cgctgaatgg ttttatcagc gaatggtaca cctggcagtc gctgttctca ctaagtcgtg
   111961 tggaagccgt agcgctacaa cttgcgggtc ctattgctat ggtaatgctg gcagtcactg
   112021 gtgggctggc agtaatgtgc ttcgtcaaaa tgtacggtat tactttctgt ggtgcgccgc
   112081 gcagtacaca cgctgaagag gcacaggaag tgccaaatac gatgatcgtc gccatgctac
   112141 tgctcgcggc actctgcgta ttaattgcgc ttagtgccag ttggctggca ccgaagataa
   112201 tgcatattgc ccatgcgttt accaataccc ctcccgtcac tgtcgccagc ggaatagcac
   112261 ttgtacccgg cacgtttcat acacgggtca ctccttcatt actgttgctg ttactactgg
   112321 cgatgccttt gctgccaggc ctttactggc tgtggtgtcg ttcgcgccgc gcagcgtttc
   112381 gtcgcacagg agatgcctgg gcatgcggct acggctggga aaacgcgatg gctccgtcag
   112441 gcaatggcgt gatgcagccg ctgcgtgtgg tcttctctgc gttatttcgt ctacgacaac
   112501 agctcgaccc tacgctgagg ctaaataaag gtcttgcgca cgtcaccgcc agggctcaga
   112561 gcacagaacc cttctgggat gagcgggtga tccgccccat cgtgagcgcc acccaacggc
   112621 tggtcaaaga aatacagcat ctgcaaagcg gcgactttcg tctctattgc ctgtatgtgg
   112681 tcgccgcact ggttgtgctg ctaatcgcta ttgccgtcta aggaaatcac catgagacaa
   112741 actctttgcg acggatatct ggtcattttt gcgttagcac aggccgtgat tctgctgatg
   112801 ctaaccccac tttttacggg tatttcccgg cagatacgcg cgcgtatgca ctcccgccgc
   112861 gggccgggga tctggcagaa ttatcgcgat atccacaaac tgtttaaacg ccaggaagtt
   112921 gcgccgacat cttcaggtct gatgttccgc ctgatgccgt gggtattaat cagcagcatg
   112981 ctggtgctgg cgatggcctt accactattt attaccgttt ccccttttgc gggcggcggc
   113041 gatctgatca cccttatcta tcttcttgcc ctgtttcgtt ttttctttgc tctttccggg
   113101 ctggataccg gaagtccgtt tgcgggagtc ggtgccagtc gcgagttgac gctcggcatt
   113161 ctggtcgaac caatgcttat tctctcactg ctggtattgg cgctgatagc aggttccacg
   113221 catatcgaga tgatcagcaa tacgctggct atgggctgga actcgccgct aaccaccgta
   113281 ctggcgttac tggcctgtgg ttttgcctgc ttcattgaga tgggaaaaat tccctttgat
   113341 gttgctgaag cagaacagga attacaggaa ggcccgctga ccgaatattc cggtgccggg
   113401 ctggcgctag cgaaatgggg gctggggctg aaacaggtcg tgatggcagc actgtttgtg
   113461 gccctgtttc tgccctttgg gcgcgcgcaa gaactttctc tcgcctgcct gctgacttca
   113521 cttgtcgtta cgctgctcaa ggttttgctg atttttgtac tggcctcaat cgcagaaaac
   113581 acgctggcac gcgggcgttt tttactcatt caccatgtga cctggcttgg cttcagcctt
   113641 gctgcgcttg catgggtctt ttggttaacc ggtctgtaag gagcactgac ggaatatgga
   113701 aaatcttgct ctgacgacgt taatgctgcc ttttatcggc gcactggtcg tttcgttttc
   113761 gccacaacgt cgggccgccg aatggggggt tttgttcgcc gcgctgacca cgctgtgcat
   113821 gttgtcgctg atctccgcgt tttatcaggc cgataaagtt gccgtcacgt tgacgttggt
   113881 caacgtgggg gatgtggcat tatttggcct ggtcattgat cgcgtgagta cgctgattct
   113941 gtttgtggtg gtgtttctcg gtttgctggt cacgatctac tccacgggtt atctgacgga
   114001 taaaaatcgc gaacacccgc ataacggcac gaatcgttat tacgcatttt tgctggtgtt
   114061 tatcggcgcg atggcgggac tggtactctc ctcgacgctg ctcggtcagt tgttgttttt
   114121 tgaaattacg ggcggctgct cctgggcgtt gatcagttat taccagagcg ataaagcgca
   114181 gcgttcagca ctaaaagcgt tgcttatcac ccatatcggt tcgttggggt tgtatcttgc
   114241 cgccgccacg ctgtttttgc agaccggaac gtttgtgctt agcgcgatga gcgagttaca
   114301 tggcgacgca cgttatctgg tttatggcgg catcctgttt gccgcgtggg ggaaatcggc
   114361 ccagctaccg atgcaagcgt ggctaccgga cgcaatggaa gcgccaacac cgatcagcgc
   114421 ctatctccac gccgcatcga tggtgaaagt gggcgtttac atttttgccc gcgccattat
   114481 cgacggcggc aatatcccgc atgtgattgg cggcgttggc atggtcatga cactggtcac
   114541 cattctttat ggctttctga tgtatttgcc acagcaggat atgaagcggt tgctggcctg
   114601 gtcgaccatc actcaacttg gctggatgtt cttcggcttg tcgctctcca tcttcggctc
   114661 gcggctggcg ctggagggta gcatcgccta catcgtcaac cacgcgttcg ctaaaagcct
   114721 gtttttcctt gtagcaggtg cgctgagtta cagctgcggc acgcgcttgt tgccgcgtct
   114781 gcgtggcgta ttgcacaccc tgccgttgcc aggcgtggat ttctgcgtgg cagcgctggc
   114841 aattaccggt gtgccgccgt tcaacggctt cttcagtaaa ttcccgctgt ttgccgccgg
   114901 ttttgcgttg tcagtggagt actggatcct gctgcccgcc atgattctgc tgatgattga
   114961 atcggtcgcc agtttcgcct ggtttattcg ctggtttggt cgcgtcgtgc cgggcaaacc
   115021 gagcgaggcc gtcgccgatg ccgcaccgct gccagggtca atgcgcctgg tgttgattgt
   115081 actgattgtg atgtcgctga tttccagcgt aatcgccgcg acctggttgc agtaaggaga
   115141 tgatgaatga ccggttctat gatcgtaaat aatctggcgg gactgatgat gctgacatcg
   115201 ctgtttgtga ttagcgtcaa aagctatcgc ctgtcatgcg gattttacgc ctgccagtcg
   115261 ctggtgctgg tgtctatttt cgccactctc tcgtgcctgt tcgccgcaga gcaactgctg
   115321 atctggtccg ccagcgcctt tatcaccaaa gtgctgctgg taccgttaat catgacttac
   115381 gctgcacgaa atattcccca gaacatcccg gaaaaagcgt tattcggtcc ggcaatgatg
   115441 gcactgctcg cggcgttaat tgtcctgctt tgcgcatttg tcgttcagcc cgtgaagcta
   115501 ccgatggcta ccgggctgaa accggcgctg gcggtagcgt taggtcattt tctgcttggc
   115561 ctgctgtgca ttgtcagcca acgcaatatc ctgcggcaaa tttttggtta ctgcctgatg
   115621 gaaaacggct cccatctggt gctggcgctt cttgcctggc gagcaccgga actggtggaa
   115681 ataggtatcg ctaccgacgc catcttcgcc gtcattgtga tggtgttact ggcaagaaaa
   115741 atatggcgta cccacggcac gctggacgtg aacaacttga ccgcgctgaa gggataatga
   115801 gatgagttat tctgtgatgt tcgctttact cctgctcacg ccgctgcttt tttcgctgct
   115861 ctgttttgcc tgccggaaac gggggctctc tgcgacttgt acggtgactg tattacatag
   115921 cttagggatc acactgctgc tgattctagc actctgggtg gtccaaactg ccgctgatgc
   115981 aggagaaata ttcgctgcgg gactgtggct tcatattgat ggtctgggcg gtttgttcct
   116041 cgccattctt ggtgtgattg gctttctcac cggtgtttac tcgattggct acatgcgtca
   116101 tgaagtggca cacggcgagc tttcacccgt tacactgtgc gattactacg gtttcttcca
   116161 tctgtttttg ttcaccatgc tgctggttgt taccagcaat aacctgattg tgatgtgggc
   116221 ggcgatcgaa gccaccacct taagctcggc gtttctggta ggcatttacg gtcagcgttc
   116281 atcgctggaa tcagcatgga agtacatcat tatttgtact gttggtgtcg cttttggtct
   116341 gttcggtacc gtgctggtat acgccaacgc cgccagcgtt atgccgcagg cagaaatggc
   116401 gatattctgg agcgaggttc ttaagcaatc gtccttgctt gacccaacat taatgctgtt
   116461 ggcctttgtg tttgtgctaa ttggctttgg taccaaaacc gggctatttc ccatgcacgc
   116521 ctggctgccg gatgctcaca gtgaagcgcc gagtccggtc agcgccctgc tctccgccgt
   116581 attgctgaac tgcgcgctgt tggtgctgat tcgctattac atcattattt gccaggccat
   116641 cggcagcgat ttccccaacc ggttgttgct catcttcggc atgttgtcgg ttgccgtggc
   116701 ggcatttttc attctggtac agcgggacat taagcgtctg ctggcgtact ccagcgtgga
   116761 gaacatgggg ctggtcgcgg tggcgctagg cattggcggg ccgctgggaa tttttgccgc
   116821 gctgctgcac accttaaacc acagtctggc aaaaacgctg ctgttctgcg gttccggcaa
   116881 tgtactgctc aagtacggca cgcgcgatct caacgtcgtc tgtgggatgc tcaaaatcat
   116941 gccatttacc gccgtgctgt ttggtggcgg tgcgctggcg ctggcgctgg cagggatgcc
   117001 gcccttcaac atttttctta gcgaatttat gaccgttacc gcaggactgg cacgtaatca
   117061 cctgctgatt atcgtcctgc tgttattgct gttaacgctg gtgctggcgg gcctggtacg
   117121 gatggctgcg cgggtgttaa tggcgaaacc gccgcaggcc gttaaccggg gtgatctcgg
   117181 ctggttgacc acctcgccaa tggtgattct gctggtcatg atgctggcga tgggaacgca
   117241 tattccacaa cctgtcatca ggatcctggc gggcgcttcc actatagtcc tctcagggac
   117301 gcacgacctg cctgcacaac gtagcacctg gcatgatttt ttgccttcag gcaccgcatc
   117361 tgtttcggag aaacacagtg aacgttaatt catcgtcaaa tcgtggcgaa gcgattctcg
   117421 ccgccctgaa aacgcagttc cccggcgcgg tgctggatga agagcgacaa acgcctgaac
   117481 aggtcaccat tacggtgaaa atcaatctgc tgcctgacgt tgtacattat ctttattatc
   117541 aacatgatgg ctggcttcca gtcctgtttg gcaacgacga gcggacactt aacggtcatt
   117601 acgcggttta ttatgccctt tcaatggaag gggccgaaaa atgctggatc gtggtgaagg
   117661 cactggtcga tgccgacagt cgggagtttc cgtcagtcac accgcgcgtc cctgccgcgg
   117721 tctggggcga gcgagaaatt cgcgatatgt acgggctgat tccggttggc ctgccggatc
   117781 agcgtcgcct ggtgttgccc gatgactggc cggaagatat gcatccgctg cgcaaagatg
   117841 cgatggatta tcgactgcgc cctgaaccga cgacagattc cgaaacgtat gcgtttatca
   117901 atgagggcaa cagcgatgcg cgggtgatcc ctgtcggccc gctgcatatc acctcctatg
   117961 aaccgggtca cttccgcttg tttgtggatg gcgagcaaat tgtcgatgct gattaccgcc
   118021 tgttttatgt ccatcgcggc atggagaaac tggcagaaac gcggatgggc tacaacgaag
   118081 tgaccttctt atcggaccgc gtgtgtggga tttgcggttt tgcccacagt gtggcctata
   118141 ccaactcggt tgaaaatgca ctggggattg aggtgccgca acgagcacat actattcgct
   118201 cgattctgct ggaagtcgag cggctacaca gtcatttgct caaccttggc ctctcctgcc
   118261 attttgttgg ttttgatacc ggctttatgc aatttttccg cgtgcgggaa aagtcgatga
   118321 cgatggcgga attgctgacc gggtcgcgta aaacctacgg tctgaatctg attggtggtg
   118381 ttcgccgcga tattctcaaa gagcaacgtc tgcaaacgct gaaactggtg cgcgagatgc
   118441 gcgccgacgt gtcggagctg gtagagatgc tgcttgctac gccgaatatg gaacaacgca
   118501 ctcagggcat tggcattctc gaccgacaaa tcgcccgtga ttatagccct gtagggccgc
   118561 tgatccgcgc agtggttttg cccgtgattt gcgctttgat cacccctacg ccgactacgg
   118621 caatattcca aaaacactgt ttacctttac cggcggcgat gttttctccc gcgtgatggt
   118681 ccgtgtcaaa gagacgtttg attcgctggc aatgctggaa tttgccctcg acaacatgcc
   118741 ggatacccca ctgctgaccg aaggctttag ctataaacct cacgcattcg cgctgggctt
   118801 tgttgaagcg ccacgcggtg aagacgtgca ctggagcatg ctcggtgata accaaaaatt
   118861 gttccgctgg cgctgtcgtg ccgccaccta cgccaactgg ccggtgttgc gttacatgct
   118921 gcgcggcaat accgtttctg acgcaccgct gattatcggt agccttgatc cctgctactc
   118981 ctgtaccgac cgtgtgacgc tggtagatgt gcgcaagcgc cagtcaaaaa ccgtgctgta
   119041 taaagagatc gaacgctacg gcattgatcg taaccgttcg ccgctgaagt aaggacagaa
   119101 gatgctgaag ttactgaaaa ctattatgcg cgccggaacc gcgacggtga aatatccctt
   119161 cgcgccactg gaggtcagcc caggctttcg cgggaaaccg gacctgatgc ccagccaatg
   119221 tattgcctgc ggtgcctgcg cctgtgcttg tccggcaaat gcgctgacta tccagaccga
   119281 cgaccagcaa aattcgcgca cctggcagct ctatctgggg cgttgtattt actgcggacg
   119341 ttgtgaagaa gtgtgcccga ccagagccat ccagcttacc aataactttg aactgaccgt
   119401 cacccataaa gccgatctct atacccacgc gacgttccat ctacaacgtt gcagccgttg
   119461 cgaacgcccg tttgccccgc aaaaaaccgt cgcactggct gctgaattgt tggcacagca
   119521 acaaaatgct ccacaaaacc gcgaaatgtt atgggcgcaa gcgagcgtct gcccggagtg
   119581 caaacaacgc gcaacgctga tcaacgacga tacagatgta ccgctggtgg ctaaggagca
   119641 gctatgagtc cagtgcttac gcaatatgtc agccagccca tcacgctgga cgagcaaacg
   119701 caaaagatga agcggcattt gctacaggat atccgtcgct cggcttacgt ttatcgcatc
   119761 gattgcggcg gctgcaacgc ctgtgaaatc gaaatttttg ctgccatcac ccccgtattc
   119821 gacgcagaac gttttggcat taaggttgtt tcatcaccgc gtcacgccga tattttgtta
   119881 tttactggcg cagtcacccg ggcgatgcgt atgcctgcac ttcgggcgta tgagtctgcc
   119941 cccgatcata aaatctgtgt ttcctacggc gcgtgcggtg tcggcggcgg tattttccac
   120001 gatctctaca gcgtctgggg cggcagcgac accattgtcc ccattgatgt ttggatcccc
   120061 ggctgcccgc caacaccggc cgccaccatt cacggtttcg ccgtggcgct cggtttgctg
   120121 caacagaaga ttcacgcggt ggattatcgc gatcccaccg gggtgactat gcaaccgttg
   120181 tggccgcaga tcccgccatc acagcgtatc gccattgagc gagaagcgcg gcggctggcg
   120241 ggctatcgtc aggggcgaga aatttgcgat cggctcctgc gccatttaag cgacgatcct
   120301 acaggaaatc gggttaacac ctggttgcgc gatgccgacg atccacgtct caatagtatc
   120361 gttcagcaac tctttcgcgt actccggggg ttacatgact gaagagtgcg gggaaattgt
   120421 tttctggacg ctgcgaaaaa agtttgtcgc cagtagcgac gagatgccgg aacacagctc
   120481 tcaggtaatg tattactcgc tggctatcgg tcatcacgtt ggcgtgattg attgtctgaa
   120541 tgtcgccttc cgctgcccac tgacggaata cgaagattgg cttgcactgg tcgaagagga
   120601 gcaagcccga cgtaagatgc tgggggtgat gacttttggt gagattgtta ttgacgccag
   120661 ccacaccgcc ctgttgaccc gggcattcgc gccactggca gatgacgcga cgtctgtgtg
   120721 gcaagcgcgt agcattcaat tcattcatct gttggatgaa attgtgctgg aaccggctat
   120781 ctatctgatg gccagaaaaa ttgcgtgaga aggatttctc attaataagg actgttgatg
   120841 gctatgtcag acgaagcgat gtttgccccg ccgcaaggaa taacaattga agcggtaaac
   120901 ggaatgctcg cggagcggtt agcgcagaaa cacggcaagg cgtctttatt acgcgccttc
   120961 atcccgctgc cgccgccgtt cagcccggta caacttattg aactgcatgt tctcaaaagc
   121021 aacttctatt accgctacca tgatgatggc agcgatgtga cggcaacaac agagtatcag
   121081 ggcgagatgg tcgattattc gcgttacgcc gtccttctcg gcagtagtgg aatggcggag
   121141 ctacgcttta ttcgtaccca cggcagccgt tttactcccc aggattgcac actgtttaac
   121201 tggctggcac gcattatcac cccggttctg caatcatggc tcaatgatga agcacagcag
   121261 gtggcgctgc gtttgctgga gaaagatcgc gatcatcatc gggtactggt ggatatcact
   121321 aatgcagtgc tgtcacatct tgatctcgac gatctgatcg ctgacgtcgc tcgtgagatc
   121381 catcattttt tcggtctggc ttcagtcagt atggtactgg gcgatcatcg aaagaacgag
   121441 aagttcagcc tgtggtgcag cgatctttct gcctcacatt gtgcgtgtct gccacacaat
   121501 atgcctggcg acagtgtatt gctgacacaa acgctacaaa cccgacaacc gaccttgacg
   121561 caccgtgcag atgatctgtt tctctggcaa cgcgacccgt tattactctt acttgcatct
   121621 aacggctgcg aatctgcgct ccttataccg cttacctttg gcaaccatac accgggtgca
   121681 ttgttgctgg cgcatacctc ttccactctc tttagtgagg aaaactgcca gctactacaa
   121741 cacatagccg atcgcatcgc tattgccgtt ggcaatgccg atgcctgccg tagaatgacc
   121801 gatttgcagg aaagtttgca gcaagaaaac caccagctta gcgagcagct cctttcgaat
   121861 ctgggcgtcg gtgacattat ctatcaaagc caggcaatgg aagacctgct ccagcaggta
   121921 gatattgtgg cgaagagcga cagtacggtg ttgatttgcg gtgaaaccgg aaccggcaaa
   121981 gaggtgatcg ccagagcgat ccatcaactt agcccgcgac acgacaagcc gctggtcaaa
   122041 atcaactgcg ctgccatccc cgccagtctt ctggaaagtg agttattcgg tcatgacaaa
   122101 ggggcgttta ctggtgcgat taatacccat cgtggtcgtt ttgaaattgc cgatggcggc
   122161 acgttgtttc tcgatgaaat tggcgatctg ccgttagaac ttcagcctaa actgctgcgc
   122221 gtattgcagg aacgggagat tgagcgtctc ggcgggagta gaacgatccc ggtaaatgtc
   122281 agagtcattg ccgccaccaa ccgtgatttg tggcaaatgg ttgaagatcg ccagtttcgc
   122341 agcgatctct tttatcgcct gaatgtcttc ccactggaat tgcctccgct gcgcgaccgt
   122401 ccggaagata tccctctttt agcaaagcat ttcacgcaaa aaatggcgcg ccatatgaat
   122461 cgttcaattg acgccatccc gaccgaggcg ctacgccagt tgatgtcgtg ggactggccg
   122521 ggcaacgtgc gcgagctgga aaacgtgatt gagcgagcgg tactgctgac tcgcggtaac
   122581 agtctgaatt tacatcttaa tgtccgacaa agccgtttac tgccgacgct aaatgaagat
   122641 tcagcgcttc gcagttcaat ggcgtagttg ctgcacccga cgacgccaga gaatgacgaa
   122701 gaagaacgtc agcgcattgt tcaggtattg cgagaaacca atggcattgt tgccgggccc
   122761 cgtggcgcgg cgacacgatt agggatgaag cgcaccacgc tgctgtcacg aatgcagcgt
   122821 ctgggaatct cggttcgcga ggtgttgtaa tctgcttttg caggagtatg catgagaaac
   122881 aaactctctt tcgacttgca gttgagcgcc agaaaagcgg caatcgctga acggattgcc
   122941 gcccataaaa ttgcccgcag taaagtgtcg gtctttttaa tggcgatgtc cgctggcgtg
   123001 tttatggcga tgtccgctgg cgtgtttatg gcgatcggat ttacttttta cctttccgtt
   123061 atcgccgatg ccccgtcttc ataggcatta acccatctgg tgggcggcct ttgctttaca
   123121 cttggcttta ttttgctggc ggtttgcggc accagcctgt tcacctcgtc ggtaatgacg
   123181 gtaatggcaa aaagtcgggg cgttattagt tggcgaactt ggctgattaa cgcacttctg
   123241 gtggcctgcg gtaatctggc aggtattgcc tgtttcagtt tgttaatctg gttttccggg
   123301 ctggtgatga gtgaaaacgc gatgtgggga gtcgcgattt tacactgcgc cgagggcaaa
   123361 atgcatcata catttactga atctgtcagc ctcggcatta tgtgcaatct gatggtttgc
   123421 ctggcgctgt ggatgagtta ttgcggacgt tcgttatgcg acaaaatcgt cgccatgatt
   123481 ttgtccatca ccctgtttgt cgccagtggc tttgagcact gtatcgccaa tttgtttgtg
   123541 attccgttcg ccattgccat tcgccatttc gcccctacct ccttctggca actggcgcac
   123601 agtagcgcag acaattttcc ggcactgacg gtcagccatt ttattaccgc caatctgctc
   123661 ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg tgagtatttg ttatcgggct
   123721 atttatttac gtcaggaatc ctgaaaaatc agcccggcga aacagttcgt cgggctgaag
   123781 attattcttg cgcgatttgc ccatcgggcc aggcgtggat cacggctttg atcagcgtcg
   123841 ccaatggaat ggcgaagaat acgccccaga atccccacaa accaccgaag atcaccaccg
   123901 ataaaataat caccagcgga tgcaggttaa ccgcttcgga gaacaacacc ggtactagca
   123961 ggttgccgtc cagcgcctga ataatcagat acaccgcgaa gcagctccag aattcagtac
   124021 ctgcgccaaa ctggaatagc gccacgccaa ccaccggaat ggtcaccaca aatgcgccaa
   124081 tgtacggaat aagaaccgag aagccgacca gcactgccag cagcagcgaa tagttcagcc
   124141 cgaagagcaa gaaccccagc caggtggcga tccccaccac gatcatctcc agcactttgc
   124201 cgcggatata gttggtgatt tgttgattca tctccttcca cacctgtcct gccagcccac
   124261 ggttacgcgg cagcacccgg cgaacggcgt tcagcatctg ctctttgtct ttcagcagga
   124321 agaagaccat caatggcacc agcaccagat agacggctat ggtcagcaaa ccgaccagcg
   124381 aggcgaggga aattttcacc accgaatcgc ccatggtcag catccgactg cgcatatttt
   124441 cggccattgc atcaataatg cccgcgtcca ttaacaccgg atagcggcgc ggcaacgtgg
   124501 cggcaaagtc agaaagctta ttgagcatcc ccggcatatc gcggattaag tagatgccct
   124561 gttgccaggc gataggcagt accacgaacg ccatcagtag caatataccg acaaaaacca
   124621 ccaatacaat cgacgtcgcc cagcggcggg agcagccaat agattgcagg cgcacggttg
   124681 gccattccag caaataagcc agcacaatag ccaccagcaa cggagcaagc aggccactaa
   124741 agaaaaagat aatgccaaat ccggcaacta aaataaccag caaggcaatc gcttccgggt
   124801 cgctaaaacg gcggcgatac cattgcatca acatttcgag cataaagacc ttccctgaac
   124861 ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt cacaaaaaag acttcgcttt
   124921 ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg ctaatcttcg ccgttacact
   124981 caaaggcggc gaggtgggaa cgatatttca cagtatcggt caaatgacta tcttcagaaa
   125041 tacaggatag aggttatgtt caggcagttg aaaaaaaacc tggttgcaac cctcattgct
   125101 gctatgacca ttggtcaggt agccccggcg tttgccgaca gcgcagacac cttgccggat
   125161 atgggaacct ccgcaggaag cacgctttcc attggtcagg aaatgcagat gggcgactat
   125221 tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg acccgctgtt aacgcaatat
   125281 attaattcgc tggggatgcg tctggtttcg catgccaatt cggttaagac accgtttcat
   125341 ttttttctga tcaacaacga cgaaattaac gcctttgctt tctttggcgg caacgtggtg
   125401 ctgcactctg ccctgttccg ttattccgat aacgaaagtc aactggcttc agttatggcg
   125461 cacgaaatct cccacgtcac ccaacgtcac ctggcgcgag cgatggaaga tcaacaacgc
   125521 agcgcgccgc tgacctgggt cggcgcgtta ggttctattt tactggcgat ggccagtccg
   125581 caggcgggga tggcggcgct gaccggtaca ctggcgggaa cgcgccaggg gatgatcagt
   125641 ttcacccagc aaaatgaaca ggaagcggac cgcattggta ttcaggtgct gcaacgctcg
   125701 ggattcgatc cacaggcgat gccgaccttc ctggaaaaat tgctcgatca ggcgcgttac
   125761 tcctcgcgcc cgccggaaat tttattgact cacccgttgc cggaaagtcg tctggcagat
   125821 gcccgcaacc gtgctaatca gatgcgcccg atggtggtgc agtcgtcgga agatttctat
   125881 ctggcgaaag cgcgcacact ggggatgtat aattccggac gtaaccagct caccagtgat
   125941 ttgctggatg aatgggcgaa aggaaacgtt cgtcagcaac gagcggcgca atatggtcgt
   126001 gctttacagg cgatggaagc caataaatac gacgaggcgc gtaaaacgct gcaaccgtta
   126061 ctggcggcag aacctggcaa cgcatggtat ctcgatctgg ctaccgatat cgatcttggg
   126121 caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg cccgcgattt acgcaccaat
   126181 ccggtgttgc agctcaacct ggcgaacgct tatctgcaag gcggtcaacc acaagaagcg
   126241 gccaatattc tgaatcgcta cacctttaat aataaagatg acagcaacgg ctgggatttg
   126301 ctggcacagg cggaagccgc gctaaataac cgcgatcagg agctggctgc gcgagcagaa
   126361 ggttatgcgc tcgccggacg actcgatcag gccatttcgc tgttgagtag cgccagttcg
   126421 caggtgaaat taggcagcct gcaacaagcg cgttacgatg cgcgcatcga ccagttgcgc
   126481 cagctgcaga aacgctttaa gccttatacc aagatgtaat tcgcattaaa ggaagaacaa
   126541 tgaccaaaca ggtaaaaatc taccataacc cacgctgttc aaagagccgg gaaacgctga
   126601 acctgctgaa agaaaacggc gtggagccag aagtggtgct ctaccttgag acacccgccg
   126661 atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat gaatagcgcc cgtgaattga
   126721 tgcgccagaa agaggatctt tataaagaac taaacctggc tgacagttcg cttagcgaag
   126781 aggcgctgat tcaggcaatg gtggataatc cgaagctgat ggagcgcccg attatggtcg
   126841 cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt actggaaatc gtgggttaag
   126901 atgtgctcca tcgctagttg aagcacattg ccggatgcga cgcttgccac gtcttatccg
   126961 gcctacgaat actatggatt aacttcgcgt aggtcaggcg ttcgcgccgc atccgacaat
   127021 aaacacctta tctacaactt cagaatttct ttcacaaacg gaatggtcag cttacgttgc
   127081 gcggtaatcg acgcacgatc caactgatcc aacgtcataa atagcgtgcg catttctctg
   127141 tcgagccgct tcagcaagaa acgccccaca tcttccggca gttcaaaacc acgcaaacgc
   127201 gcgcgtaact gtagcgcctg caacttatct tcatcagaaa gtggctgcaa tttgtagatc
   127261 tgcccccagt cgagacgcga cgcgagatcc ggtaatccca gattcaactg ccgcggtgga
   127321 cgatcgccgg tgatcaacaa ccgtgttttg cccgattcca gaattcgatt gtagagatcg
   127381 aaaatcgcca tctcccacaa ctcatcgcct gcaatacact caatgttgtc gatacagacc
   127441 agcgacaaat gctccatacc gtcgagcact tccggaacaa accaggtgcg tttatccagc
   127501 gggacatagc ccaccgcatc gccacgctgc gacaattccg cgcaagccgc gtgcagcaga
   127561 tggctgcgcc ccgcgccttc gcgtgcccag agatagatgt aaccgctatg ttcctgacgc
   127621 agcacgtttt gcagcgcggc cagtaaagag gagttatccc ccggccagaa acttgcaaag
   127681 gtttcgtcgt caggaagata aagtggcaaa gagagctgtg ccggtgtgtt cagagatacc
   127741 tcaaccagga tttcacaaaa tcgcgagaag tttaccacag aatcccatga tgtttgaacc
   127801 gggcagcaac actgcccggt cggtacatta tttgtctgtt atatccgcgt cttctgcgtc
   127861 cagcaccact tcttcagggc gcagcacact gatcagtttg aaaatcaaac ttaagccgat
   127921 accgacgatg gtcgccagcg ccataccttt taactccgcc gcgccgatgt ttaccttcgc
   127981 gccactgacg ccgatgatca aaatcacgga agtcaggatc aggttctgtg ctttgttgta
   128041 atccactttc gattcgatca aaacacgaat accggaagca ccaatgacac cataaagcag
   128101 cagcgaaacg ccgcccatca ccggcaatgg gatcatctgg atagcggcag ccagtttacc
   128161 gacgcaggaa agcaggatag cgaaaatcgc cgccccgccg ataacccagg tactgtaaac
   128221 acgggtgatc gccatcacgc caatgttttc tccgtaagtg gtatttggcg tagagccaaa
   128281 gaagccggag atcacggtcg acaggccgtt agcaaacatc gaacggtgca gacctggatc
   128341 gcgcagcaga tcttttttga cgatattagc cgttactacc aggtgcccta cgtgttcggc
   128401 aataaccact aacgccgctg gcagaatagt cagaatggca aaccactcga agcgcggcgt
   128461 atagagggtt ggcagtgcaa accagtgagc attaataatc ggcgtggtat cgacaattcc
   128521 cattgcgaaa gagagcgcgt accccaccag cacgccaatt aaaatcggga taattgccag
   128581 gaaaccacga aacagcacgg aacctaaaac cgtgaccgcc agggtggtaa tagagatgat
   128641 gatggttttg gagtttggcg tttgcccttc agccgggagt aaacccgcca taccggcagc
   128701 tacgcccgcc agctccagac cgatgacggc aacgattgcg cccattgccg caggtggaaa
   128761 cagcacgtcc aaccagccgg tccccgcttt cttcacgata aaagaaacca ggcagaacag
   128821 cacgccgcac ataataaagc cgcccagcgc gacttcatac cctaacggca acagtaacaa
   128881 taccggtgaa ataaaggcaa agctggaacc aagataagcc ggaattttcc ctttacagat
   128941 gaagagatac agcagcgttc caataccgtt aaataacagt acagtcgccg ggttaatatg
   129001 aaataagacg ggcaccagga cggttgcacc aaacatggcg aacaaatgtt gcaaactaag
   129061 cgggattgtc tgtaaaagtg gcggtctttc actcaccccg atagcacggc gcgtcatagt
   129121 attatcctct gtattatgtg ttataggcgc tttactcaaa aaaaagccga ctcttaaagt
   129181 cggctttaat tatttttatt ctttatttcg taccaaagat tttgtcaccg gcatcgccga
   129241 ggcccggaat aatgtatccg tgctcgttca gtccctggtc aatcgatgca gtatacagtt
   129301 cgacgtccgg gtgcgctttt tccagcgcag cgataccttc tggcgcagcg accagcacca
   129361 gaactttgat gctgctgcag cccgcttttt tcagcaggtc gatggtcgcg ataacggaac
   129421 caccggttgc cagcattggg tcaacgatca gcgccatacg ctcatcgatg ttagaaacca
   129481 gtttctggaa gtacggtacc ggctccagcg tttcttcatt acggtacata ccgacaacgc
   129541 tgatgcgcgc gctcggaacg ttttccagca caccgtccat cataccaaga cccgcacgca
   129601 gaattggcac aacggtaatt ttcttacctt tgatctggtc gatttctacc gggccgttcc
   129661 agccttcgat agttactttt tccgtttcga ggtcggcggt cgcttcgtaa gtcagcaggc
   129721 tacccacttc ggaagcgagt tcgcgaaagc gcttggtgct gatatcttgc tcacgcatca
   129781 gtcccagctt gtgtttgacg agtgggtgtt tgacttccac gatcttcata ctctttctcc
   129841 tttgaggggc agccacaaaa aaatcgacgg attatacctc ctttcttcag ggcggcaata
   129901 ttattttcgt tgactttagt caaaatgata acagtttgag ataaagttat tttatattca
   129961 aatggttatg aaagaagatt attccatccg aaaactaacc tttgccctgg cacaagtctt
   130021 ctttcgccgc gcgcccgggg aaaagacgtg caaaaaggtt gtgtaaaaca gtctcgcaaa
   130081 cgtttgcttt ccctgttaga attgcgccga attttatttt tctaccgcaa gtaacgcgtg
   130141 gggacccaag cagtgaccga taaaacctct cttagctaca aagatgccgg tgttgatatt
   130201 gacgcgggta atgctctggt tggaagaatc aaaggcgtag tgaagaaaac gcgtcgtccg
   130261 gaagtgatgg gcggtctggg cggcttcggt gcgctgtgtg cattgccgca aaaatatcgt
   130321 gaacccgtgc tggtttccgg cactgacggc gtaggtacca agctgcgtct ggcaatggac
   130381 ttaaaacgtc acgacaccat tggtattgat ctggtcgcca tgtgcgttaa tgacctggtg
   130441 gtgcaaggtg cggaaccgct gtttttcctc gactattacg caaccggaaa actggatgtt
   130501 gataccgctt cagcggtgat cagcggcatc gcggaaggtt gtctgcaatc gggctgttct
   130561 ctggtgggtg gcgaaacggc agaaatgccg gggatgtatc acggtgaaga ttacgatgtc
   130621 gcgggtttct gcgtgggcgt ggtagaaaaa tcagaaatca tcgacggctc taaagtcagc
   130681 gacggcgatg tgctgattgc actcggttcc agcggtccgc actcgaacgg ctattcgctg
   130741 gtgcgcaaaa ttcttgaagt cagcggttgt gatccgcaaa ccaccgaact tgatggtaag
   130801 ccattagccg atcatctgct ggcaccgacc cgcatttacg tgaagtcagt gctggagttg
   130861 attgaaaagg tcgatgtgca tgccattgcg cacctgaccg gcggcggctt ctgggaaaac
   130921 attccgcgcg tattgccaga taatacccag gcagtgattg atgaatcttc ctggcagtgg
   130981 ccggaagtgt tcaactggct gcaaacggca ggtaacgttg agcgccatga aatgtatcgc
   131041 accttcaact gcggcgtcgg gatgattatc gccctgcctg ctccggaagt ggacaaagcc
   131101 ctcgccctgc taaatgccaa cggtgaaaac gcgtggaaaa tcggtatcat caaagcctct
   131161 gattccgaac aacgcgtggt tatcgaataa tgaatattgt ggtgcttatt tccggcaacg
   131221 gaagtaattt acaggcaatt attgacgcct gtaaaaccaa caaaattaaa ggcaccgtac
   131281 gggcagtttt cagcaataag gccgacgcgt tcggccttga acgcgcccgc caggcgggta
   131341 ttgcaacgca tacgctcatc gccagcgcgt ttgacagtcg tgaagcctat gaccgggagt
   131401 tgattcatga aatcgacatg tacgcacccg atgtggtcgt gctggctggt tttatgcgca
   131461 ttctcagccc ggcgtttgtc tcccactatg ccgggcgttt gctgaacatt cacccttctc
   131521 tgctgccgaa atatcccgga ttacacaccc atcgtcaggc gctggaaaat ggcgatgaag
   131581 agcacggtac atcggtgcat ttcgtcaccg atgaactgga cggtggcccg gttattttac
   131641 aggcgaaagt cccggtattt gctggtgata cggaagatga catcaccgcc cgcgtgcaaa
   131701 cccaggaaca cgctatttat ccactggtga ttagctggtt tgccgatggt cgtctgaaaa
   131761 tgcacgaaaa cgccgcgtgg ctggatggtc aacgtctgcc gccgcagggc tacgctgccg
   131821 acgagtaatg cccccgtagt taaagctcca gctctgccgc tggcgttttt caattcacct
   131881 gtaaatcgca agctccagca gtttttttcc cccttttctg gcatagttgg acatctgcca
   131941 atattgctcg ccataatatc caggcagtgt cccgtgaata aaacggagta aaagtggtaa
   132001 tgggtcagga aaagctatac atcgaaaaag agctcagttg gttatcgttc aatgaacgcg
   132061 tgcttcagga agcggcggac aaatctaacc cgctgattga aaggatgcgt ttcctgggga
   132121 tctattccaa taaccttgat gagttctata aagtccgctt cgctgaactg aagcgacgca
   132181 tcattattag cgaagaacaa ggctccaacg ctcattcccg ccatttactg ggcaaaattc
   132241 agtcccgggt gctgaaagcc gatcaggaat tcgacggcct ctacaacgag ctattgctgg
   132301 agatggcgcg caaccagatc ttcctgatta atgaacgcca gctctccgtc aatcaacaaa
   132361 actggctgcg tcattatttt aagcagtatc tgcgtcagca cattacgccg attttaatca
   132421 atcctgacac tgacttagtg cagttcctga aagatgatta cacctatctg gcggtggaaa
   132481 ttatccgtag cgatagcatc cgttacgcgc tgctggagat cccatcagat aaagtgccgc
   132541 gctttgtgaa tttaccgcca gaagcgccgc gtcgacgcaa gccgatgatt cttctggata
   132601 acattctgcg ttactgcctt gatgatattt tcaaaggctt ctttgattat gacgcgctga
   132661 atgcctattc aatgaagatg acccgcgatg ccgaatacga tttagtgcat gagatggaag
   132721 ccagcctgat ggagttgatg tcttccagtc tcaagcagcg tttaactgct gagccggtgc
   132781 gttttgttta tcagcgcgat atgcccaatg cgctggttga agtgttacgc gaaaaactga
   132841 ctatttcccg ctacgactcc atcgtccccg gcggtcgtta tcataatttt aaagacttta
   132901 ttaatttccc caatgtcggc aaagccaatc tggtgaacaa accactgccg cgtttacgcc
   132961 atatttggtt tgataaagcc cagttccgca atggttttga tgccattcgc gaacgcgatg
   133021 tgttgctcta ttatccttat cacacctttg agcatgtgct ggaactgctg cgtcaggctt
   133081 cgttcgaccc gagcgtactg gcgattaaaa ttaacattta ccgcgtggcg aaagattcac
   133141 gcatcatcga ctcgatgatc cacgccgcac ataacggtaa gaaagtcact gtggtggttg
   133201 agttacaggc gcgtttcgac gaagaagcca acattcactg ggcgaagcgc ctgaccgaag
   133261 caggcgtgca cgttatcttc tctgcgccgg ggctgaaaat tcacgccaaa ctgttcctga
   133321 tttcacgtaa agaaaacggt gaagtggtcc gttacgcaca catcgggacc gggaacttta
   133381 acgaaaaaac cgcgcgtctt tatactgact attcgttgct gaccgcagat gcgcgtatca
   133441 ccaacgaagt acggcgggta tttaacttta ttgaaaaccc ataccgcccg gtgacatttg
   133501 attatttaat ggtgtcaccg caaaactctc gccgtctgtt atatgaaatg gtagaccgcg
   133561 aaatcgccaa cgcgcagcaa gggctgccca gtggtatcac cctgaagcta aataaccttg
   133621 tcgataaagg cctggttgat cgtctgtatg cggcctccag ctccggcgta ccggttaatc
   133681 tgctggttcg cggaatgtgt tcgctgatcc ccaatctgga aggcattagc gacaacattc
   133741 gtgccatcag tattgttgac cgttaccttg aacatgaccg ggtttatatt tttgaaaatg
   133801 gcggcgataa aaaggtctac ctttcttccg ccgactggat gacgcgcaat attgattatc
   133861 gtattgaagt ggcgacaccg ctgctcgatc cgcgcctgaa gcagcgggtg ctggacatca
   133921 tcgacatatt gttcagcgat acggtcaaag cacgttatat cgataaagaa ctcagtaatc
   133981 gctacgttcc ccgcggcaat cgccgcaaag tacgggcgca gttggcgatt tacgactaca
   134041 tcaaatcact cgaacaacct gaataaccct atgccaatac acgataaatc ccctcgtccg
   134101 caggagtttg ctgcggtcga tcttggttca aacagttttc acatggtcat agcccgtgtg
   134161 gtagatggtg ccatgcagat tattggccgc ctgaaacagc gggtgcatct ggcggacggc
   134221 ctggggccag ataatatgtt gagtgaagag gcaatgacgc gcggtttgaa ctgtctgtcg
   134281 ctgtttgccg aacggctaca agggttttct cctgccagcg tctgtatagt cggcacccat
   134341 acgctgcgtc aggcgctgaa cgccactgac tttctgaaac gcgcggaaaa ggtcattccc
   134401 tacccgattg aaattatttc cggtaatgaa gaagcccgtc tgatttttat gggcgtggaa
   134461 catacccaac cggaaaaagg tcgcaaactg gttattgata ttggcggcgg atctacggaa
   134521 ctggtgattg gtgaaaattt cgaacctatt ctcgttgaaa gccgccggat gggttgtgtc
   134581 agctttgccc agctttattt tcctggcggg gtcatcaata aagagaattt tcagcgcgct
   134641 cgcatggcgg cagcacaaaa actggaaact ttaacctggc aattccgtat tcagggctgg
   134701 aacgttgcaa tgggcgcttc cggtaccata aaagccgccc atgaagtgtt aatggaaatg
   134761 ggcgagaaag acgggataat taccccggaa cgtctggaaa aactggtaaa agaaatttta
   134821 cgtcaccgta atttcgcatc gctgagttta ccgggtcttt ccgaagagcg gaaaacagtc
   134881 ttcgttcctg gactggcgat tttatgtggt gtgtttgatg ctttagccat ccgtgaactg
   134941 cgcctttctg acggggcgtt acgcgaaggc gtactgtatg aaatggaagg acgtttccgt
   135001 catcaggatg tgcgtagtcg caccgccagc agcctcgcca accagtatca catagacagc
   135061 gaacaggccc gacgagtgct ggataccact atgcaaatgt acgaacagtg gcgggaacag
   135121 caaccgaagc tggcgcatcc gcaactggag gcgctactgc gatgggccgc catgctgcat
   135181 gaggtcgggt tgaatatcaa ccacagcggt ttgcatcgcc actccgctta tattctgcaa
   135241 aacagtgact tgccgggttt taatcaggaa cagcagctga tgatggcgac actggtgcgc
   135301 tatcaccgta aagcgattaa gctcgacgat ctgccgcgct ttaccttgtt taagaagaaa
   135361 cagttcctgc cactgataca gctattgcgc cttggcgtat tactaaacaa tcaacgtcag
   135421 gcaaccacca caccgccaac attgacactg attactgatg acagtcactg gacactgcgt
   135481 ttcccgcatg actggtttag tcagaatgcg ctggtactgc ttgatctgga aaaggagcaa
   135541 gaatactggg aaggcgtggc tggctggcgg ttgaaaattg aagaagaaag taccccagaa
   135601 atcgccgctt aatgctttgc gggccgacga gaaatgtcgg cccgcattat tcaggcactt
   135661 tcgcgaatgg gttcgatttc attcagcgta tcaattaacg gttgcggctt accaataaga
   135721 taaccctgca tataatcgat ccccaaagag agcaccgcct cgcggatctc ttcgttttca
   135781 acgtactctg ccactacccg cattttcttc attcgcgcca gatggcaaat cgacgccaca
   135841 atctgataat ccagactatt tgacacaata ttgcggataa agctgccgtc aattttaagc
   135901 agatcggcat tcacattttt aagccgtgca tagctggcgt agccggtgcc gaaatcatca
   135961 atcgcaatct ggcagcctaa ttcctgaaga tgctgcaagg tgatttgcgc ctgcttaaca
   136021 ttggtcagag cattactttc ggtgacttca aaaataagtt gccacgcttc aatctgatat
   136081 ttagccagca actgactgac ttcaacagga aaacgagcct gacataccga ggttggagac
   136141 agattaatag caaaacggtg agcgggcatc ttcgctctgt tttcagccat aaattgcagc
   136201 gtatgctcaa tgacccacat gtcgatactc gacgataaac caaattcgtg cgcgaccggc
   136261 aagaagctac cggggccgat cagttcatca ttctcacctt tcatgcgcag aagaatttca
   136321 tggtaaacat caccacgcat accggtaatc ggctgggcca tcaggaaaaa atggttgtgt
   136381 tccagcgcct gctgtagtcg attcatcatc gcgactttat ctttcaattc gcgttgcaaa
   136441 tacattgccc cgcaacgctg catattttcc ggggcgttgg tcacgatgga aagttcggcg
   136501 accgtattta gctctcccag cagcaggtag atatgatcca ctggcgagcg cacatagcag
   136561 taactgacgc caatctgcgg ttgcatcggc atcccatccc aaaagaaacg aaattgcttg
   136621 ag
//
LOCUS       DAJTPG010000002        95515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTPG010000002 DAJTPG010000000
VERSION     DAJTPG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO230_000132"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO230_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5690034.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OO230_000133"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OO230_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5690035.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OO230_000134"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OO230_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5690036.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OO230_000135"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OO230_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5690037.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OO230_000136"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OO230_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5690038.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OO230_000137"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OO230_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5690039.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OO230_000138"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OO230_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5690040.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OO230_000139"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OO230_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5690041.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OO230_000140"
     CDS             complement(8942..9223)
                     /locus_tag="OO230_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690042.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OO230_000141"
     CDS             complement(9237..10898)
                     /locus_tag="OO230_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5690043.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OO230_000142"
     CDS             complement(10882..11208)
                     /locus_tag="OO230_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5690044.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLEQYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OO230_000143"
     CDS             complement(11264..11446)
                     /locus_tag="OO230_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690045.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OO230_000144"
     CDS             complement(11430..11870)
                     /locus_tag="OO230_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5690046.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OO230_000145"
     CDS             complement(11870..12166)
                     /locus_tag="OO230_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5690047.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="OO230_000146"
     CDS             complement(12163..12501)
                     /locus_tag="OO230_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5690048.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OO230_000147"
     CDS             complement(12498..13709)
                     /locus_tag="OO230_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5690049.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OO230_000148"
     CDS             complement(13711..14283)
                     /locus_tag="OO230_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5690050.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OO230_000149"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OO230_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="OO230_000150"
     CDS             complement(15781..16005)
                     /locus_tag="OO230_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690051.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16147..16308)
                     /locus_tag="OO230_000151"
     CDS             complement(16147..16308)
                     /locus_tag="OO230_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5690052.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16318..16623)
                     /locus_tag="OO230_000152"
     CDS             complement(16318..16623)
                     /locus_tag="OO230_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690053.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="OO230_000153"
     CDS             complement(16620..16898)
                     /locus_tag="OO230_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690054.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="OO230_000154"
     CDS             complement(17187..18935)
                     /locus_tag="OO230_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5690055.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="OO230_000155"
     CDS             complement(18932..19231)
                     /locus_tag="OO230_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5690056.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="OO230_000156"
     CDS             complement(19237..19470)
                     /locus_tag="OO230_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690057.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="OO230_000157"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="OO230_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="OO230_000158"
     CDS             complement(19685..19906)
                     /locus_tag="OO230_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690058.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="OO230_000159"
     CDS             complement(19896..20096)
                     /locus_tag="OO230_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5690059.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="OO230_000160"
     CDS             20226..20405
                     /locus_tag="OO230_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690060.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="OO230_000161"
     CDS             20530..20859
                     /locus_tag="OO230_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690061.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="OO230_000162"
     CDS             complement(20909..21097)
                     /locus_tag="OO230_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5690062.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="OO230_000163"
     CDS             complement(21472..22701)
                     /locus_tag="OO230_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5690063.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22950..24071
                     /gene="roxA"
                     /locus_tag="OO230_000164"
                     /gene_synonym="ycfD"
     CDS             22950..24071
                     /gene="roxA"
                     /locus_tag="OO230_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5690064.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO230_000165"
     CDS             complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO230_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5690065.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25596..26732
                     /gene="potA"
                     /locus_tag="OO230_000166"
     CDS             25596..26732
                     /gene="potA"
                     /locus_tag="OO230_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5690066.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26716..27573
                     /gene="potB"
                     /locus_tag="OO230_000167"
     CDS             26716..27573
                     /gene="potB"
                     /locus_tag="OO230_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5690067.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27570..28364
                     /gene="potC"
                     /locus_tag="OO230_000168"
     CDS             27570..28364
                     /gene="potC"
                     /locus_tag="OO230_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5690068.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28361..29407
                     /gene="potD"
                     /locus_tag="OO230_000169"
     CDS             28361..29407
                     /gene="potD"
                     /locus_tag="OO230_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5690069.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO230_000170"
     CDS             complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO230_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5690070.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO230_000171"
     CDS             complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO230_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5690071.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO230_000172"
     CDS             complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO230_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5690072.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO230_000173"
     CDS             complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO230_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5690073.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO230_000174"
     CDS             complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO230_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5690074.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34739..35812
                     /locus_tag="OO230_000175"
     CDS             34739..35812
                     /locus_tag="OO230_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5690075.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35940..39386
                     /gene="mfd"
                     /locus_tag="OO230_000176"
     CDS             35940..39386
                     /gene="mfd"
                     /locus_tag="OO230_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5690076.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39529..40491
                     /gene="ldtC"
                     /locus_tag="OO230_000177"
     CDS             39529..40491
                     /gene="ldtC"
                     /locus_tag="OO230_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5690077.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40574..40786)
                     /locus_tag="OO230_000178"
     CDS             complement(40574..40786)
                     /locus_tag="OO230_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5690078.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41071..41703
                     /gene="comR"
                     /locus_tag="OO230_000179"
     CDS             41071..41703
                     /gene="comR"
                     /locus_tag="OO230_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5690079.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41765..42304)
                     /locus_tag="OO230_000180"
     CDS             complement(41765..42304)
                     /locus_tag="OO230_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5690080.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO230_000181"
     CDS             complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO230_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5690081.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44243..44785)
                     /locus_tag="OO230_000182"
     CDS             complement(44243..44785)
                     /locus_tag="OO230_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5690082.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO230_000183"
     CDS             complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO230_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5690083.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO230_000184"
     CDS             complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO230_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5690084.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO230_000185"
     CDS             complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO230_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5690085.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47305..47682)
                     /locus_tag="OO230_000186"
     CDS             complement(47305..47682)
                     /locus_tag="OO230_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5690086.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO230_000187"
     CDS             complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO230_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5690087.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48383..50568
                     /gene="fhuE"
                     /locus_tag="OO230_000188"
                     /pseudo
     CDS             48383..50568
                     /gene="fhuE"
                     /locus_tag="OO230_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50628..52061)
                     /gene="ptsG"
                     /locus_tag="OO230_000189"
     CDS             complement(50628..52061)
                     /gene="ptsG"
                     /locus_tag="OO230_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5690088.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52356..53153)
                     /locus_tag="OO230_000190"
     CDS             complement(52356..53153)
                     /locus_tag="OO230_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5690089.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53164..54168)
                     /gene="holB"
                     /locus_tag="OO230_000191"
     CDS             complement(53164..54168)
                     /gene="holB"
                     /locus_tag="OO230_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5690090.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54165..54806)
                     /locus_tag="OO230_000192"
     CDS             complement(54165..54806)
                     /locus_tag="OO230_000192"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5690091.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54796..55818)
                     /gene="yceG"
                     /locus_tag="OO230_000193"
     CDS             complement(54796..55818)
                     /gene="yceG"
                     /locus_tag="OO230_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5690092.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55821..56630)
                     /gene="pabC"
                     /locus_tag="OO230_000194"
     CDS             complement(55821..56630)
                     /gene="pabC"
                     /locus_tag="OO230_000194"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5690093.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56750..57991)
                     /gene="fabF"
                     /locus_tag="OO230_000195"
     CDS             complement(56750..57991)
                     /gene="fabF"
                     /locus_tag="OO230_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5690094.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58078..58314)
                     /gene="acpP"
                     /locus_tag="OO230_000196"
     CDS             complement(58078..58314)
                     /gene="acpP"
                     /locus_tag="OO230_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5690095.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58525..59259)
                     /gene="fabG"
                     /locus_tag="OO230_000197"
     CDS             complement(58525..59259)
                     /gene="fabG"
                     /locus_tag="OO230_000197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5690096.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59272..60201)
                     /gene="fabD"
                     /locus_tag="OO230_000198"
     CDS             complement(59272..60201)
                     /gene="fabD"
                     /locus_tag="OO230_000198"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5690097.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60217..61170)
                     /gene="fabH"
                     /locus_tag="OO230_000199"
     CDS             complement(60217..61170)
                     /gene="fabH"
                     /locus_tag="OO230_000199"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5690098.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61238..62308)
                     /gene="plsX"
                     /locus_tag="OO230_000200"
     CDS             complement(61238..62308)
                     /gene="plsX"
                     /locus_tag="OO230_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5690099.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62389..62562)
                     /gene="rpmF"
                     /locus_tag="OO230_000201"
     CDS             complement(62389..62562)
                     /gene="rpmF"
                     /locus_tag="OO230_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5690100.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62614..63135)
                     /gene="yceD"
                     /locus_tag="OO230_000202"
     CDS             complement(62614..63135)
                     /gene="yceD"
                     /locus_tag="OO230_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5690101.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63334..63918
                     /gene="yceF"
                     /locus_tag="OO230_000203"
     CDS             63334..63918
                     /gene="yceF"
                     /locus_tag="OO230_000203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5690102.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64030..64989)
                     /gene="rluC"
                     /locus_tag="OO230_000204"
     CDS             complement(64030..64989)
                     /gene="rluC"
                     /locus_tag="OO230_000204"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5690103.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65562..68753
                     /gene="rne"
                     /locus_tag="OO230_000205"
     CDS             65562..68753
                     /gene="rne"
                     /locus_tag="OO230_000205"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5690104.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68950..69892)
                     /gene="flgL"
                     /locus_tag="OO230_000206"
                     /pseudo
     CDS             complement(68950..69892)
                     /gene="flgL"
                     /locus_tag="OO230_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69904..71546)
                     /gene="flgK"
                     /locus_tag="OO230_000207"
                     /pseudo
     CDS             complement(69904..71546)
                     /gene="flgK"
                     /locus_tag="OO230_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71612..72553)
                     /gene="flgJ"
                     /locus_tag="OO230_000208"
     CDS             complement(71612..72553)
                     /gene="flgJ"
                     /locus_tag="OO230_000208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5690105.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72553..73650)
                     /gene="flgI"
                     /locus_tag="OO230_000209"
     CDS             complement(72553..73650)
                     /gene="flgI"
                     /locus_tag="OO230_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5690106.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73662..74360)
                     /gene="flgH"
                     /locus_tag="OO230_000210"
     CDS             complement(73662..74360)
                     /gene="flgH"
                     /locus_tag="OO230_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5690107.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74413..75195)
                     /gene="flgG"
                     /locus_tag="OO230_000211"
     CDS             complement(74413..75195)
                     /gene="flgG"
                     /locus_tag="OO230_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5690108.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75333..76084)
                     /gene="flgF"
                     /locus_tag="OO230_000212"
                     /pseudo
     CDS             complement(75333..76084)
                     /gene="flgF"
                     /locus_tag="OO230_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76104..77312)
                     /gene="flgE"
                     /locus_tag="OO230_000213"
     CDS             complement(76104..77312)
                     /gene="flgE"
                     /locus_tag="OO230_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5690109.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77337..78032)
                     /gene="flgD"
                     /locus_tag="OO230_000214"
     CDS             complement(77337..78032)
                     /gene="flgD"
                     /locus_tag="OO230_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5690110.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78044..78447)
                     /gene="flgC"
                     /locus_tag="OO230_000215"
                     /pseudo
     CDS             complement(78044..78447)
                     /gene="flgC"
                     /locus_tag="OO230_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78451..78867)
                     /gene="flgB"
                     /locus_tag="OO230_000216"
     CDS             complement(78451..78867)
                     /gene="flgB"
                     /locus_tag="OO230_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5690111.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79022..79681
                     /gene="flgA"
                     /locus_tag="OO230_000217"
     CDS             79022..79681
                     /gene="flgA"
                     /locus_tag="OO230_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5690112.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79757..80050
                     /gene="flgM"
                     /locus_tag="OO230_000218"
     CDS             79757..80050
                     /gene="flgM"
                     /locus_tag="OO230_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5690113.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80055..80471
                     /gene="flgN"
                     /locus_tag="OO230_000219"
     CDS             80055..80471
                     /gene="flgN"
                     /locus_tag="OO230_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5690114.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80511..82046)
                     /gene="murJ"
                     /locus_tag="OO230_000220"
     CDS             complement(80511..82046)
                     /gene="murJ"
                     /locus_tag="OO230_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5690115.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82246..83079)
                     /locus_tag="OO230_000221"
     CDS             complement(82246..83079)
                     /locus_tag="OO230_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5690116.1"
                     /translation="MKKLRIGVVGLGGIVQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83081..83728)
                     /locus_tag="OO230_000222"
     CDS             complement(83081..83728)
                     /locus_tag="OO230_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5690117.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83739..84323)
                     /gene="rimJ"
                     /locus_tag="OO230_000223"
     CDS             complement(83739..84323)
                     /gene="rimJ"
                     /locus_tag="OO230_000223"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5690118.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84559..85767
                     /gene="mdtH"
                     /locus_tag="OO230_000224"
     CDS             84559..85767
                     /gene="mdtH"
                     /locus_tag="OO230_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5690119.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85831..86478
                     /gene="grxB"
                     /locus_tag="OO230_000225"
     CDS             85831..86478
                     /gene="grxB"
                     /locus_tag="OO230_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5690120.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86612..87172
                     /locus_tag="OO230_000226"
     CDS             86612..87172
                     /locus_tag="OO230_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5690121.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87278..88324
                     /gene="pyrC"
                     /locus_tag="OO230_000227"
     CDS             87278..88324
                     /gene="pyrC"
                     /locus_tag="OO230_000227"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5690122.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88398..88643
                     /gene="dinI"
                     /locus_tag="OO230_000228"
     CDS             88398..88643
                     /gene="dinI"
                     /locus_tag="OO230_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5690123.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88933..89187
                     /gene="bssS"
                     /locus_tag="OO230_000229"
     CDS             88933..89187
                     /gene="bssS"
                     /locus_tag="OO230_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5690124.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89302..90420
                     /gene="solA"
                     /locus_tag="OO230_000230"
     CDS             89302..90420
                     /gene="solA"
                     /locus_tag="OO230_000230"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5690125.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90468..90581
                     /locus_tag="OO230_000231"
     CDS             90468..90581
                     /locus_tag="OO230_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5690126.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90842..91408
                     /locus_tag="OO230_000232"
     CDS             90842..91408
                     /locus_tag="OO230_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5690127.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91412..91987
                     /locus_tag="OO230_000233"
     CDS             91412..91987
                     /locus_tag="OO230_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5690128.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(92029..93081)
                     /locus_tag="OO230_000234"
     CDS             complement(92029..93081)
                     /locus_tag="OO230_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5690129.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93306..94226
                     /gene="lpxL"
                     /locus_tag="OO230_000235"
     CDS             93306..94226
                     /gene="lpxL"
                     /locus_tag="OO230_000235"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5690130.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94398..94595
                     /locus_tag="OO230_000236"
     CDS             94398..94595
                     /locus_tag="OO230_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5690131.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94619..>95515
                     /locus_tag="OO230_000237"
     CDS             94619..>95515
                     /locus_tag="OO230_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5690132.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLF"
BASE COUNT        22981 a        25312 c        23566 g        23656 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tactgttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22921 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22981 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    23041 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
    23101 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
    23161 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg
    23221 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    23281 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    23341 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    23401 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    23461 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
    23521 cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa
    23581 ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    23641 cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    23701 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    23761 ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag
    23821 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
    23881 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23941 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    24001 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    24061 tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct
    24121 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    24181 ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    24241 gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc
    24301 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
    24361 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag
    24421 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    24481 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    24541 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
    24601 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    24661 cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg
    24721 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    24781 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    24841 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    24901 atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta
    24961 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    25021 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    25081 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    25141 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    25201 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    25261 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    25321 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    25381 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    25441 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    25501 ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt ggttaaccac
    25561 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    25621 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    25681 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    25741 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    25801 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    25861 gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa
    25921 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25981 tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct
    26041 ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag cctcgtctgt
    26101 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    26161 agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg
    26221 aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag
    26281 acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg
    26341 gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg
    26401 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    26461 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    26521 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    26581 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    26641 atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    26701 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    26761 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    26821 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa ctacacgcgt
    26881 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26941 accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac
    27001 aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt
    27061 cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    27121 tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc
    27181 ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    27241 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    27301 atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg
    27361 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    27421 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc
    27481 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    27541 cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    27601 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    27661 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    27721 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    27781 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    27841 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga
    27901 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27961 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
    28021 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    28081 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    28141 taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt
    28201 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    28261 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    28321 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    28381 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    28441 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    28501 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    28561 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    28621 cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    28681 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
    28741 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    28801 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
    28861 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28921 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    28981 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    29041 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
    29101 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    29161 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    29221 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
    29281 agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    29341 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    29401 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    29461 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt
    29521 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa
    29581 cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc
    29641 attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc
    29701 ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac
    29761 atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    29821 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    29881 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    29941 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    30001 cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc
    30061 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    30121 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    30181 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    30241 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    30301 aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa
    30361 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    30421 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg
    30481 agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa
    30541 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
    30601 cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    30661 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata
    30721 cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc
    30781 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
    30841 atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag
    30901 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    30961 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    31021 atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc
    31081 ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc
    31141 tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc
    31201 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    31261 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    31321 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    31381 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    31441 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg
    31501 agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg
    31561 aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat
    31621 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    31681 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    31741 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    31801 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    31861 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    31921 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    31981 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    32041 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    32101 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    32161 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    32221 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac
    32281 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca
    32341 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca
    32401 ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    32461 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    32521 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    32581 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    32641 ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc
    32701 ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    32761 gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
    32821 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    32881 cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt
    32941 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    33001 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag
    33061 tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc
    33121 taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc
    33181 accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg
    33241 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    33301 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    33361 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc
    33421 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    33481 caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga
    33541 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    33601 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca
    33661 ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    33721 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    33781 caactctcag cggctcatcc agccacaaac gccagccggt aatattggct gccggataac
    33841 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    33901 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
    33961 tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact
    34021 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    34081 tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca
    34141 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    34201 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    34261 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    34321 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    34381 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt
    34441 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    34501 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    34561 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat tttatcatca
    34621 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    34681 tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat
    34741 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    34801 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    34861 agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt
    34921 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    34981 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    35041 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    35101 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
    35161 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    35221 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    35281 tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg
    35341 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    35401 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    35461 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    35521 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    35581 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    35641 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    35701 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    35761 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac
    35821 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    35881 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    35941 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    36001 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    36061 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    36121 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    36181 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    36241 tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    36301 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    36361 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    36421 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    36481 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    36541 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    36601 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    36661 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc
    36721 agccattgtt cttcagcgaa ccactgccac cgctgttcag ttatttccct gccaatacct
    36781 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    36841 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    36901 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa
    36961 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    37021 tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    37081 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    37141 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    37201 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc
    37261 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    37321 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    37381 agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag
    37441 ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg
    37501 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    37561 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    37621 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    37681 tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa
    37741 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    37801 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    37861 ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc
    37921 ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    37981 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    38041 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    38101 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    38161 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    38221 gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    38281 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    38341 cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa
    38401 acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg
    38461 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    38521 gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca
    38581 acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg
    38641 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    38701 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    38761 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    38821 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    38881 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    38941 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga
    39001 acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    39061 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    39121 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    39181 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    39241 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    39301 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    39361 aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc
    39421 aacatttaca aattctttgc actttcctgc actatccgac actgtcacca tccataattc
    39481 aggcttatct gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa
    39541 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    39601 ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac
    39661 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    39721 gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg
    39781 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    39841 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    39901 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    39961 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    40021 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    40081 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    40141 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    40201 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    40261 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    40321 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    40381 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    40441 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    40501 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    40561 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    40621 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    40681 ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt
    40741 ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg
    40801 ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    40861 gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    40921 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt caaatagaaa
    40981 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    41041 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    41101 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    41161 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga
    41221 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    41281 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    41341 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    41401 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    41461 ggagatatag ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    41521 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    41581 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    41641 ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    41701 taagtcgcaa aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    41761 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    41821 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    41881 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    41941 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    42001 cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc
    42061 caccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    42121 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    42181 gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca
    42241 ctgccgctac gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat
    42301 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    42361 ctatcagaca gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag
    42421 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    42481 ttgtccaggg cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac
    42541 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    42601 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    42661 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    42721 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc
    42781 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    42841 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    42901 gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata
    42961 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    43021 cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    43081 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    43141 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    43201 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc
    43261 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    43321 ttcactttgc catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc
    43381 tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct
    43441 ggcgtattga aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt
    43501 ttacgttccg gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt
    43561 ttcgcttcac gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc
    43621 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    43681 tgcagcagtg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    43741 ttcttacgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    43801 ccgacaatca caatcttttt caatggcgta gtcaacgtgc cccccttaaa tttattaacc
    43861 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    43921 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    43981 atcaagtttt gttgcttaaa agataggcaa ctttaccggg ggagatcaaa aaatagagtt
    44041 tattgtttat tatttgagca aattcggaag ggtaatatag ggtgaagcgt atatcgaggg
    44101 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    44161 atgcggcgtg gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
    44221 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
    44281 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
    44341 gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa
    44401 ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
    44461 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    44521 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    44581 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
    44641 ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    44701 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    44761 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctact
    44821 ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga
    44881 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    44941 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    45001 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
    45061 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaccccagt
    45121 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
    45181 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    45241 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    45301 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    45361 attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga
    45421 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
    45481 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
    45541 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
    45601 aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc
    45661 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    45721 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    45781 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    45841 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    45901 atcgccggtt tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg
    45961 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    46021 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
    46081 cgcgatatca ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg
    46141 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc
    46201 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc
    46261 gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg
    46321 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    46381 taccgcaccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    46441 tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    46501 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
    46561 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    46621 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    46681 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    46741 tgccagaggc gcaggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
    46801 ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    46861 acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
    46921 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    46981 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    47041 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt
    47101 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    47161 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    47221 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    47281 tttttgtcat caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc
    47341 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg
    47401 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg
    47461 aacggtaacg ggttcttgcc tttcgttata aatcgttgag gatgctgaag gttgaatatc
    47521 agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat
    47581 caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag
    47641 taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc
    47701 gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct
    47761 tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct
    47821 tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct
    47881 gacacgtcgt tcacagtcgg aatgagaata ttcggaatga tcagaatatg cgttggcgct
    47941 tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg
    48001 atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc
    48061 aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg
    48121 agttaaacct gctctttcaa tcttaactca cgatttctta agcaaaaaat cgttttgctg
    48181 ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg
    48241 tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttgc atttgcagca
    48301 aaattattcg cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac
    48361 aacataaacc aagagatttc aaatgctttc aacacaattt aacagggata atcaatatca
    48421 agtcatcacc aaaccgtcac tacttgccgg ttgcatagca ctggcactat taccttctgc
    48481 cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt gagggttcag ccacagctcc
    48541 agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat
    48601 gactcaacat gatattcctc agtcggtcac tattgttagc cagcagcgga tggaagatca
    48661 gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagtcaggc
    48721 ggattccgat cgtgctcttt attattcccg cggattccag atcgataact atatggttga
    48781 tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc
    48841 actgtttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa
    48901 tccatctgcg gcaattaata tggttcgaaa acacgcgacc tgtcgtgaat ttaaaggcga
    48961 tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc
    49021 actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga ataacgactc
    49081 atggctggac cgctacaata gtgaaaagac cttcttctcg ggcattgtcg atgctgattt
    49141 aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc
    49201 aacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgcgc
    49261 acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg tctttatgac
    49321 cctgaagcag cggtttgctg atacctggca agcgacactg aatgccaccc actctgaagt
    49381 cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct
    49441 ggttgggcca tacagtaatt atggacctgg ctttgatcat gtcggcggca ccggttggaa
    49501 cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtagtt atgaattgtt
    49561 tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttcagt
    49621 tcatgggcca acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc
    49681 ccacaagccg actggtcatc acagagcctg gcgcaggacg ataccacaca tatgaaatcg
    49741 ttatatgctg ccactcgtgt cacccttgcc gatccgctgc atctgatcct cggcgcacgt
    49801 tataccaact ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct
    49861 tacgctggtc tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct
    49921 attttccagc cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt
    49981 aacaactacg agctgggtct gaaatcggac tggatgaata gccgtctgac caccacgtta
    50041 gccatcttcc gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc
    50101 agcaacggcg aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt
    50161 gaactcaacg gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatctt
    50221 gcagaggata acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg
    50281 ttcaccagct atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgttaactgg
    50341 caaaatcgcg tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt
    50401 agctacgcgc tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag
    50461 gggaacgtca ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc
    50521 tacggcgcac cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta
    50581 aaaaaggcag ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg
    50641 atgtactcat ccatctcggt tttcaggtta tcggatttag taccgaaaat agcctgaaca
    50701 ccagaaccag caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact
    50761 ttagacacat cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg
    50821 ttttctttac caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct
    50881 tttgcatctt cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc
    50941 acgcggaaga tggtgtagta aacaatcgca taaccgatac cgacgatcgg taacagccac
    51001 agtttgctgc tgttaccaga cagaacgatg aagtcgatca gaccgtgcga gaacgacgta
    51061 ccgtcacgca tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg
    51121 atgatgtaca ggatcggcgc aacgaacatg aagaagaact cgatcggctc ggtgataccg
    51181 gtcaggaacg aggtcagcgc cgcggagatc ataataccgc ccactttcgc gcggttttct
    51241 ggtttagcag agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg
    51301 aagccaccag acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg
    51361 tggaaaacct gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc
    51421 cagatgtggt gcagaccaaa cggtaccagg caacgttcga tgaaaccgta aatgccaaac
    51481 gcaactaccg ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc
    51541 ggcggccaaa tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc
    51601 ggcacaaagc gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa
    51661 cggttaaaca tgtacgctgc aatcgcaccg gagataatcc ctccgagtac gccagtatcc
    51721 gccaagtgtt tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg
    51781 gccatggttt taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg
    51841 ttattggtaa agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg
    51901 gaaccgcctg cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg
    51961 gaaccgacgc ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat
    52021 ttaccgacct tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag
    52081 tatgggtgct ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag
    52141 ggcatctaag cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg
    52201 acggctatca cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat
    52261 gtcacaatat ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt
    52321 tttagccatt aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa
    52381 gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg
    52441 gcaacacctt tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc
    52501 tcttttcctc gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga
    52561 tccaggggga cataacgcgc agcatcgcgc agttgctccg cattacggaa ggtcacaatg
    52621 ccggaaaagg agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct
    52681 gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc
    52741 gtatcggcac gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc
    52801 tggatatgat ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa
    52861 tccagcccgg tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct
    52921 tctacatcgt agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg
    52981 tcacgttcgc ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc
    53041 agacaaaatt tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc
    53101 aaagattcat aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct
    53161 ctcttaaaga tgaggaaccg gtaacacaac gcccggttgc aggtaatgct caatacgcag
    53221 taaaagatcg gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg
    53281 aatgtggcaa acatccccca gtatagcctg caggcacgag ggagaaagat ggtttgccag
    53341 ttcggcgacc aggcccggca catcaacatt ggtcacctgc gcagcgccat gatggcgttt
    53401 tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc gccggagctt gttcatgatt
    53461 aagggccgcc agcagcgaat accagtcgcc cgatggcacg ctatatgcca acgcctgaca
    53521 caatgtttca cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgccaggcga
    53581 accggcgctt aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga
    53641 aagccaagtc acggcgtact gttctggagg cggcgcaagg taatgtaacc gacaacgact
    53701 acgtaatgtt gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc
    53761 tggtggctct tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc
    53821 atcggttacc cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt
    53881 gacctcacgt accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta
    53941 gtaatcggga tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt
    54001 gtggccctgc ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc
    54061 gcccatgccc ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct
    54121 ggctaccagt ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc
    54181 ttcacccagt gggtcacggt agtgcggatt gcatccatca cggcctccag cggctgggtg
    54241 gcatcaatgg tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg
    54301 cgattaaaga aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg
    54361 cgttttaagc caacttccgg ggtaacatcg agatagagcg ttaagtctgg gcgaaaatcc
    54421 ccgagaacag catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc
    54481 tgatacgcct gagtggaaag atcgtggcga tcgccaatca cccaggtgcc gttagccagc
    54541 gcaggtttga tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg
    54601 gctttatcgg taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac
    54661 ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt
    54721 tgctcgagcg tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc
    54781 tcaatgacga tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac
    54841 agacttgtta tgactggcaa gattggtatt aaacgtgtga ccacctttac catcggccac
    54901 aaaatagaga tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg
    54961 cgtagctatc gcacccggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt
    55021 ttccaggtct gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt
    55081 tgggtcggtc tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg
    55141 atcgcgttca ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg
    55201 atttttatct ttataaggca gaccgtccgc acgcccttcc caggcgctat cgaccgcttt
    55261 caccattttc ttgtgaactc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat
    55321 caaggtgtct ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg
    55381 cgctacggtg gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa
    55441 ttgcttgagg tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc
    55501 tttaccgctt tccaacaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta
    55561 agtcccggct ttaaagtgag aaagatccgg ttcgatacgc agcagccatt gaaaaacccg
    55621 tgggcgattg atgatcttat cagcataaag ctgttcaccg agcgccagac gtccggtccc
    55681 tggcttcagg gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg
    55741 aaccttccag acgcccacac cagcggcgat acccagtacc accagcaata acaagataat
    55801 caataacact tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat
    55861 aacgttgctg acgaaaaggt gacatcgcca caggcacgta cgggcatcac tggcattaac
    55921 gcattacaaa taaccatctc atctgcctgc aacgcctcct ccagcgaggc ttgcacttcg
    55981 acaagctgat aagaggattg tgccagtaaa cggatacaga attgtcgcat aatgccgtta
    56041 acacctgcct gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta
    56101 gccgcacagc attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt
    56161 gtctgctcaa gatgagagcg aatcaatact tgctcaagcc gattaagatg tttaatacct
    56221 gcaagatgag gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta
    56281 cgcaaacggt cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt tgctggtccg
    56341 ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca
    56401 ccattttgct gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca
    56461 caggaaatca tcaaccgctg acaagcatcc tgtagtcgca ggatatgcgc cgataacaaa
    56521 ctgactttac cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc
    56581 cgatcgctta ctgccagcga ttcctgctta taaccgttaa ttaagaacat accggctcct
    56641 tatcagagaa ttgttagtgt ggcagcatgt tcactacgga acaagtcgga ataaaagcta
    56701 agaaaaaagg cccgcaagcg gaccttttat atgggtggaa aatgacaact tagatctttt
    56761 taaagatcaa agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca
    56821 ttccgctgac ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccgggt
    56881 tatccaggtt gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag
    56941 attctactgc acccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca
    57001 acacacggct tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca gctttatcgc
    57061 cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg
    57121 catcacgcag agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg
    57181 tcatatgata agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac
    57241 cgcgtttttt cgcgtgttcg tactcttcaa gtaccagcat accggcacca tcgcccagta
    57301 cgaagccatc acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg
    57361 tagataatgc acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg
    57421 cgccacctgc aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatggccaa
    57481 tgttgtgcac gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat
    57541 acatgatagt cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc
    57601 tgatcttacg tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc
    57661 cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa
    57721 ggccagaatc ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca
    57781 tcttgcgctg ttctttgcgc gagataatgt cctcacagtt aaaatccttt actaagccag
    57841 caaatttcgt tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac
    57901 cggcaagcag agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca
    57961 gtccggtcac aactacacga cgcttagaca cgtttgtcct ccaaggaggg aaaaatgatt
    58021 ctagtgggac aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta
    58081 cgcctggtgg ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc
    58141 ttcgtccgga atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc
    58201 aagagaatcc gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt
    58261 aacgcccagc tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt
    58321 aaatttccta tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa
    58381 tttgcaacta aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc
    58441 ccaataacgc aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac
    58501 atgagcattt tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt
    58561 ttcacccgtg atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc
    58621 ctgtgcgccg ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc
    58681 gctcagcgca cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat
    58741 accgcgtgac gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc
    58801 cgcagcgtag ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat
    58861 aatacgacca tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac
    58921 agatgaaagg ttagtttcga taatatcgtt ccactcttca tctttcattc gcattaacag
    58981 gttatcacga gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg
    59041 aattttttcc agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt
    59101 gccgttggca cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc
    59161 aataactttc gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct
    59221 tgcaccggtt accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc
    59281 gagcgccgct gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat
    59341 gcgtttcgtc aggccagtaa gcaccttgcc cgggccgact tcatagagat gttctacgcc
    59401 ttgcgctgcc atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac
    59461 cagtgcgtca cggatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac
    59521 agaaactgtt ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc
    59581 tggtttcatc agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc
    59641 gccagcagct ttacaggcag cgccagcacg ctcaaccgct tctttatgac cggcaataac
    59701 cacctgtccc ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc
    59761 acacgctttc gcaataggcg catcatccag accgatgatt gccgccatag cgcccgtgcc
    59821 ttccggtacg gcttcttgca tgaacttacc gcgcatctca accagacgca ccgcatcagc
    59881 gaaatcaatc acgccagcgc aaaccagcgc ggagtattcc cccaggctgt gaccggccat
    59941 cattgccggt gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt
    60001 caacagcgct ggctgggttt gccaggtttt attcagttct tcagctgacc cctgctgggt
    60061 cagcgcccac aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat
    60121 tggatagctc gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa
    60181 cacaaatgca aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcagagcc
    60241 ccaagtgaat ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc
    60301 gtcgcgtaca gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg
    60361 atccagcgtc accacgacat tgtccatcga cataccgagt tttttcgccg ttgcactgat
    60421 aatacgcagg ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt
    60481 cgccgccagc gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc
    60541 gttgcccgcc atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag
    60601 cgtcagcaat tcaccataac taccgtcggc atgcagatgg gtggaaatga ttcccggctc
    60661 ttcagaggca gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg
    60721 atctgttgga tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac
    60781 cgccccagat ttcacgtatt gatcggctac gcttaatgca taggtgaaac ctgcacaggc
    60841 tgctgcaacg tcaaatgccg ggcaaccttt aatgtccaac atgctttgaa tctgacaagc
    60901 tgcgctcggg aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc
    60961 tttctcaatg cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac
    61021 ggtttcgttt ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc
    61081 gtcagaggtg tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata
    61141 gctgccagta ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc
    61201 gtcacttgca aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc
    61261 caccgtccag cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct
    61321 gaggaacttg tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct
    61381 gattggctgc accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt
    61441 tatactggtc ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct
    61501 ttaataacag tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa
    61561 ggaacatcct gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac
    61621 aaaccagcac atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat
    61681 tgatagaagg gattgtttta agcaccgctg aggcatcccg aatactgtca agacccttta
    61741 cttcttcttc accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt
    61801 cagccagaac tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt
    61861 tggcccctaa atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca
    61921 gcgccggacg ctcaatcccc tccaggggct tgagtaataa ttttgccagc cccatcagtg
    61981 ccccggtatt accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg
    62041 ccatacgcat tgaactccca cggctggcgc ggatagcttg cgaaggccgg gcatcactgg
    62101 cgataactga ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag
    62161 caagtaatgg cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag
    62221 aattcagtgc ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga
    62281 catctaacgc cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc
    62341 agttgcctgg ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat
    62401 gaccttgcgg ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc
    62461 agaagttttg tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat
    62521 gccacgtttg gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt
    62581 acggtttatc cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac
    62641 ggcaaatggg tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc
    62701 ggacacttca cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc
    62761 atcttcaacc attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata
    62821 cgcttccggc agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt
    62881 gtagacctga tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt
    62941 cgcatcgccg tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc
    63001 cacatcactg tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata
    63061 gataccctgg taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa
    63121 ttttaccttt tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa
    63181 ggcagcctgt ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt
    63241 tcgttcagtg ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct
    63301 atcatcaata cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc
    63361 tcgccatggc gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacct
    63421 gaggtcgacg aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca
    63481 caagaaaaat cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac
    63541 caggtatgtg ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt
    63601 ctgcaattac gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt
    63661 aattcggcga atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac
    63721 ctaagcgagg cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt
    63781 agctttaaga gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct
    63841 aacacgctgg ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa
    63901 aacccgctga tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt
    63961 gcaggcggcc tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat
    64021 atgcttgtat tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat
    64081 cggcgcttcg atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc
    64141 gtgcaggaac agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg
    64201 gtcaccgtag cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat
    64261 ctgatgagta cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc
    64321 ttccacttta aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc
    64381 gccgctttgc agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca
    64441 ctgaccgcgc accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg
    64501 cagagaacgc aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc
    64561 gagacgatga accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat
    64621 aacgccgaag cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac
    64681 caggatatga tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg
    64741 tggcgaaacc gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc
    64801 acccgcttcg agtttatatt caggcttaat acgttttttg ttcacccgca cttcaccttt
    64861 acgcaaaata cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt
    64921 atcgatacgt tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc
    64981 tgttttcatg gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg
    65041 cttatattta gttttgcacc ttattacttc actacgtgat cactttattg atggttatta
    65101 aaccaatcac cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt
    65161 catcttgcta taacaaggct tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt
    65221 taaaacaaga aattttacgg aataacccat tttgcccgac cgatcatcca cgcagcaatg
    65281 gcgtaagacg tattgatctt tcaggcagtt agcgggctgc gggttgcagt ccttaccggt
    65341 agatggaaat atttctggag agtaataccc agtctgtttc tttgataatt gcgctgtttt
    65401 tccgcatgaa aaacgggcaa ccgacactct gcgcctcttt gagctgacga taaccgtgag
    65461 gttggcgacg cgactagaca cgaggccatc ggttcacacc cggaaaggcg ttactttgcc
    65521 cgcagcttag tcgtcaatgt aagaataatg agtaagttac gatgaaaaga atgttaatca
    65581 acgcaactca gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc
    65641 tggatatcga aagtccaggg cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca
    65701 cccgcattga accgagtctg gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt
    65761 tcctcccact aaaagaaatt gcccgcgaat atttccctgc taactacagt gctcatggtc
    65821 gtcccaacat taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag atcgataaag
    65881 aagagcgcgg caacaaaggc gcggcattaa ccacctttat cagtctggcg ggtagctatc
    65941 tggttctgat gccaaataac ccgcgcgcag gtggcatttc tcgccgtatc gaaggcgatg
    66001 accgtaccga attaaaagaa gcactggcaa gccttgaact gccggaaggc atggggctta
    66061 tcgtgcgcac cgctggcgta ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc
    66121 gcctgaaaca ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga
    66181 ttcatcagga gagcaacgta atcgttcgcg ccttccgcga ttatttacgt caggatatcg
    66241 gcgaaatcct tatcgataac ccgaaagtcc tcgaactggc acgtcagcat atcgctgcat
    66301 taggtcgccc ggatttcagc agcaaaatca aactgtacac cggcgagatc ccgctgttca
    66361 gccactacca gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc cgtctgccat
    66421 ccggtggttc cattgttatc gacagcaccg aagcgttaac agccatcgac atcaactccg
    66481 cacgcgcgac ccgcggcggc gatatcgaag aaactgcgtt taacaccaac ctcgaagccg
    66541 ccgatgagat tgctcgtcag ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact
    66601 tcatcgacat gacgccagta cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg
    66661 tgcgtcagga ccgtgcgcgt attcaaatca gccatatttc tcgctttggc ctgctggaaa
    66721 tgtcccgcca gcgcctgagc ccatcactgg gtgaatccag ccatcacgtc tgcccgcgct
    66781 gctccggtac cggtaccgtg cgtgacaacg aatcgctgtc gctctctatt ctgcgtttga
    66841 tcgaagaaga agcgctgaaa gagaacaccc aggaagttca cgccattgtt cctgtgccaa
    66901 tcgcttctta tctgctgaat gaaaaacgtt ctgcggtgaa tgcgattgaa acgcgtcagg
    66961 acggtgttcg ctgcgtgatt gtgccaaacg atcagatgga aaccccgcac taccacgtgt
    67021 tgcgcgtgcg caaaggggaa gaaacgccga ccttaagcta catgctgccg aagctgcatg
    67081 aagaagcgat ggcgctgccg tctgaagaag agttcgctga acgtaagcgt cctgaacaac
    67141 ctgcactggc aacctttgcc atgccggatg taccgccagc gccaacccca gctgaacctg
    67201 ccgcgactgt cgtagcccca gcaccgaaag ctgcaacggc aacaccagca gctcctgcac
    67261 aacctgggct gttaagccgc ttcttcggcg cactgaaagc gctgttcagc ggtggtgaag
    67321 aagccaaacc gaccgagcaa ccaacaccga aagcagaagc gaaaccggaa cgtcaacagg
    67381 atcgtcgcaa acctcgtcag agcaaccgcc gtgaccgtaa tgagcgccgc gacacccgta
    67441 gtgaacgtac tgaaggcagc gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac
    67501 agcagcaaac tgccgagacg cgtgagagcc gtcagcaggc tgaggtaacg gaaaaagcac
    67561 gtaccaccga cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata
    67621 aacgtcaggc gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa
    67681 ccgaacagga agaacgtgta cgtccggttc agccgcgtcg taaacagcgt cagctcaatc
    67741 agaaagtgcg ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg gtggttgaag
    67801 aaactgccgc tgccgaacca attgttcagg aagctccagc tccacgcaca gaactggtga
    67861 aagtcccgct gccagtcgta gcgcaaactg caccagaaca gcaagaagag aacaatgctg
    67921 ataaccgtga caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg
    67981 tgagtggtca gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag tcgccaatgc
    68041 cgttgaccgt agcgtgcgcg tctccggaac tggcctctgg caaagtctgg atccgctatc
    68101 caattgtacg tccgcaagat gtacaagttg aagagcagcg cgaacaggaa gaagtacaag
    68161 tgcagccgat ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc
    68221 cagttgttga agaaatggct gaagtcgtag aagcccccgt accggttgcc gaaccgcaac
    68281 cggaagtggt tgaaacgacg catcctgaag tgattgccgc cgcggtaact gaacagccgc
    68341 aggtgattac cgagtctgat gttgccgtag cccaggaagt cgcagaacac gcagaaccga
    68401 tggttgaacc gcaggaagag acggcagatg cagatattga agaagttgcc gaaactgctg
    68461 aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc gtcgctgaag
    68521 tcgcagcaga agttgaaacg gtggccgcgg ttgaaccaga gatcaccgtt gagcataacc
    68581 acgctaccgc gccaatgacg cgcgctccgg caccggaata tgttccggag gcaccgcgtc
    68641 acagtgactg gcagcgccct acttttgcct tcgaaggtaa aggtgccgca ggtggtcata
    68701 cggcaacgca tcatgcctct gccgctcctg cgcgtccgca acctgttgag taataattag
    68761 ctcaaagtaa tcaagccctg gtaactgcca gggctttttt atttcatgtt tgaatcaaca
    68821 tattaaaatt catcaaatgg catccttgct aaccaacaat gcaaaatagg caaaaaaaat
    68881 gcccgccccg gcgatcccag agcgggcaga caaaacatac ccagtttcat gatatgtttt
    68941 aaagcgagct tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt
    69001 atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc
    69061 cacatcaacc agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc tacctaagga
    69121 atccagcgac tccagtttgt tcagctgcgt gcctaattcc gcgcgaacgg taaggacatt
    69181 attcagcgag tttttcagtc cacggttggt tttatccaat gccgcagcgg cgatttcttt
    69241 atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc
    69301 aaaaagattg gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact
    69361 gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg
    69421 cttaatactt tctgttccgc caacataatc accgttagcc tcactaaacg gcgcagtctc
    69481 tgttttatat gtagcgccca ttaccgtcag tggtgttcgc cagattcagc aactggtcac
    69541 gaagcccctg aatatccgta gccagcgagg cccggtcatc gtcactcaag gtgccattgc
    69601 tggcgtagac aattttttcc tgagcattct ggatagcagt ggtgacctgg ctaagtacac
    69661 tctcttccag tgacactttt tgagtggcga aagtacgtgc cagcgtgtac tggctgtttt
    69721 gcgcctgtgc ctgggagaga acaacagctt gtgatgcagc aatgggatcg tctgaagggt
    69781 taacgactcg cttacccgtc gacatctgct cgccgtactt catccattct gcctgagaat
    69841 tggtgatgcc gcgcatgttt tgctggtaca tcatctgggt actgaaacgc attttatctc
    69901 cccttagcga atgttaatca gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc
    69961 attcgccagg taatactgct gaaaacgttg cagatttccg tactcctcat cgagattgac
    70021 accggaaatc gactgctgct gattggaaag ctgcgtcacc acattacctt gcgtggtgct
    70081 actggttttc aacgtcgcgg ttttattacc gatatcactc actaacgagg cataagcgtc
    70141 gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt tgcagatcca gcagggcctg
    70201 accgttgcgg ttgtcgctat cacccgcatc ttcttcgctc gccatcgcga ttttcgcttc
    70261 gtcggtgatt aatacatcca tgttgacgat ggcgtcactt actggtttca gcgtgaagct
    70321 gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga ccatcaaatg ccactttacc
    70381 gttggcatct ggtgtcgccg taaaagtggt attgctggca aggcgggtga cctgccactg
    70441 attattatcg aacgagattt tgtaatctgt cgccagtacc acggaggcat cagttaccgt
    70501 ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat
    70561 agtaaagaaa tcttcaccag catcgccgtt agcatcaaat ccggctttgt gttggctgtt
    70621 gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta ttacgcgtct ggtccagatc
    70681 ctgagaacgg aatgtcagaa tgccgcccag cgacccggta ttcagtaatt tctccgggat
    70741 ctcaatattg cctgccgtcc catcaacgta agcgacagtc gtacgagaag ggtcggcgct
    70801 ggaaggaact gccgccagtt gccgcgccgt acttccctga accagtgagt aaccattggc
    70861 atcgtgatgt tataagtacc gccatcctga acgctgactt ctacgccaac aatctggttt
    70921 aattcgctca ccagttgatc gcgttgatcc agcagattgt taggtgacgc ccctgccccc
    70981 acgcctgtca ggcgcgagat ttgatcgttc aggctggcaa tttgtttagc gtaattgttg
    71041 atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc gcgcagatat
    71101 tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag cgcctggcgc
    71161 gccgccgggt cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa atcctgcatc
    71221 tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga catctgctca
    71281 tagcgggcgg tcaggccgct actttgcgtc tgcgcagcac gtaactggtt ggtaataaac
    71341 gtatcatact cacgctgcac accagaaacg tagacgccat tgccaaccca gccgccagcg
    71401 cccaacgtgc tattggcctg cgccataata gtggtttggc gggtatatcc ggcaacgtta
    71461 tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt cagtccgctc
    71521 atggcgttat tgatcaagct ggacatggag gttccttata agccttcaat tacagagtat
    71581 tatcggcagc gacccgccgg acttgagtta ttcagaacag attatcaatg ttcatgctgt
    71641 aggttttgct caccttgtcg cttatcgatt tcatctgctg gatcatgttg gtgagtttac
    71701 gggcatagtg aggatcggta gcatagcccg cgtcctgtag gacctgcgcc ccctgttccg
    71761 cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg tgttaacagc ccaacgtaat
    71821 ccgacaatgc ttccagatac gagctgtaga cccgaaactt cgcttttact ttcttcgctt
    71881 cgccgttttc atattcagtc gtggtgattt cagtgacttg ccctttccag ttgccagagg
    71941 ctttgacacc aaacaggtta tagcttggct cgccgttttc gcggcggatt tgccgttgcc
    72001 cccaaccaga ttcaagcgca gcctgagcga ggatcaaatg atgtggcaca ccgctttgct
    72061 ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa tgctctactg tcacccggca
    72121 gcgaatcatc gtagttacgt ggtacggcct tttgcaccag ctgcgaaagc gtctggtttt
    72181 gataacgcac cacggtttcg agcgggaatt tcatcggtgc tgctggcgtg gactcctctg
    72241 gcaatggttg ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc agacctttgc
    72301 ccgccgtcat ctgttgggca atttgctggt catacatact ggtatacaga cgagtgtgct
    72361 cgctgctgaa caggccatct tttggtaaag cgtcgcgcat gcttttcaac atcatctgca
    72421 cgaacatccc ttccacctga cgggccaccg gacggatatt tgccgccgga tcttcgctcg
    72481 ctttcgcctt tagttcgttg agtgattgcg catcccaggc cgcacttgcc agtagtttgc
    72541 tgtcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac tttgcattga
    72601 ttgcaaaata gacatcagat ccatcggcgt agcgcccagc gcattgagcg cacgcaccac
    72661 gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc
    72721 gatctgcgtt tgtggagtaa caacagtctg tccaccacca aacggtgtat ctggctggct
    72781 gacattggcc tgacgattaa ctgttactga gagatttccc tgcgctaccg cgcagctgtc
    72841 gagggtcact tcgcgattca tcaccaccga accggtgcgc gagttaatca ctactttagc
    72901 gtcctgcggg gtgacattaa cctgcatatt ctggatatcg gcaaggaaac ggacctggga
    72961 actgttgcca cttggtacgc gcacctgaat agtccgcgca tctaacgcgg tggcgctgcc
    73021 atatccacgc acgcggttga tggtgtcagc gatttgctgc gccatgctga aatcttcgtc
    73081 gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggcaatt cacgttcaat
    73141 aaccgcacca ttggtgatcc gtccaccgtt cagttggtta acctgaacac tgctaccgcc
    73201 tgcggaggct cctgcgccgc caaccagaat attgccctgc gccagcgcat acacctgact
    73261 gtcaacgccc ttaagcggag tcatcaacaa cgtacctcca cgcaagcttt tggcattacc
    73321 catggaagaa accaccacat cgatggtttg cccctgacgt ccaaacggag gaagtgacac
    73381 tgtcaccatt accgcagcga cgttttttag ctgcatattg gtgcccgtcg gaacggtaat
    73441 tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct gggttgtctg
    73501 gtcaccagtg ccatccagcc ccactaccaa gccatagcca atcagtgagt tttgccttac
    73561 cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg taaccagtag
    73621 aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg cgacaggtta
    73681 aggaagaaac gctgcaacca gcccatattt tgcgcttcgt taatgtagcc attgcctacg
    73741 tattcaatac gcgcatccgc cacctgagta gacggtacgg tattgctgcc gctgatagtg
    73801 cgtggattaa ccacgcccga gaagcgaata aattcggtac cctgattaat ggcaatctgt
    73861 ttttcaccca ccacatgcag gttgccgttg accagtacct ggtcaaccgt caccgtcaac
    73921 gtgccgctaa aggtattgct ggcattggcc ccgccctttc cgttgaacgt gttaccaccg
    73981 gaggcttcga catcggcacg agcgttacca aacagcccct gcaaatagcg cggcacagta
    74041 tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct tttgctggcg
    74101 ctgacgttct cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg tcgacgatct
    74161 tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat agaaccgttg
    74221 gcgacgggcg tcggaccggg aaccggttgt gcactggtcg ccccctgcac cagcggcgtg
    74281 gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat ggcataagta
    74341 tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg tgaacctgcc
    74401 accggttatg ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg tggacaccgc
    74461 tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt cttccgccac
    74521 gttgacgtta gacgtttcaa catacccttg atacagcagt cccgcgccgt tcaggcccgg
    74581 cgtgctttcg ttcggtgcac cggaggattg cgtttcggtg tagaggtttt cgccaatgct
    74641 ctccagtccg gtatcattca taaaggtggt gagattgagc tgcccaacct gaaccggagc
    74701 tgcctggcct tgttgggtta cgctgaccac gccatcacga ccgatggtga tacttaacgc
    74761 attcgccgga atggtgatcg ctggctgcac ctgaaaacca ccagccgtca ccagctgccc
    74821 gttctgatcc acctggaaag agccgtcacg gatataggct gatgagccat ctggcaacat
    74881 tacctggaaa aagccctgcc ctttaatcgc gacatctttg ctgttattgg tctgcgacag
    74941 attgccctgg ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc cgatttgtaa
    75001 tccggagggt aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa tggtttgata
    75061 aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga cgtttgccag
    75121 gttgttggca atgacgtcca tattggtttg ctgggcgtca aggcccgttt tggcgatcca
    75181 taatgaactg atcatataat gtcctgtagt ggattgctga caagcagagc agaagcacgc
    75241 gacagtgtga atttcccctc accctaaccc tctccccaaa ggggcgaggg aatagtccgc
    75301 gcgcttttat tgcaatgtca tcaatcgttc aattaactca tcgacagcag ttggttggca
    75361 cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg
    75421 ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaaccccc
    75481 gacatcacac gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct
    75541 gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc
    75601 agtttcagac gccctactgg cgcaactgta tttgccggat cgcccggatt gagcgccgag
    75661 attgtgccat cggcagcaat cgtgatttcc gccccttccg gcacagcaat tggccccgcc
    75721 tcgcctatca ccggatgccc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg
    75781 ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca tcctgctgca
    75841 acgcaacgtc cagcgggcga gaggtgtaat ccatcttgcc gggcgtcatg tctgcacccg
    75901 gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc ggcactgcgc
    75961 gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc attggccaga ttactggcgg
    76021 ttaccgcctg ttgattcagc gtctggctgg ctgctcccat cgcagtataa attgcgtgat
    76081 ccattgagct atcccgtcag cgattagcgt aagttaaccc gcgtgttgag gatctggtcc
    76141 tgggttttga tggtctgggc gttagactta tagttacgct gggcaacgat catattgacc
    76201 agttctttac tgagatcgac gttggacgct tccagcgcac cgttggtcag agtgccaaag
    76261 tttcccgtcc cggctgtccc caacagcgcc acgccagaag attgcgtcgc agaccagacg
    76321 ttgtcgcctt cggatgccag accttcgttg ttggcaaagt tcgccagtac aatctgcccc
    76381 agcagttggg tttgttcgtt ggaattgttg ccgacaaccg taccgtcatc attgatttga
    76441 taactcacca gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat gttgttagcg
    76501 ccggtatttt gctgcatgga gttgaggaag ctcagactaa acgtggcggg ttctgcaccg
    76561 ttaattgcgc cggttgcgat attattcgcc atcgcaccat ccactaatgt gccattagca
    76621 ttaaattcca gtgttgtcgc tgtcttcgca atgctgtttg gatcactgct atcctgggtg
    76681 tagacctgcc agttattatc cccggtcttc acaaaataga cgctcatgtc atgagcatta
    76741 ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc attgctggcg
    76801 ctgaatggcg taacagtagg aagcggatca ctggaattca ggttgatctg catcgacgcc
    76861 gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt cggattcgcc
    76921 ccttgctgaa tagtcggcgg cgtaccggtt accgggtaac ccgtcagctg taaaccttgc
    76981 gtattcaaca ggttacggtt ttcatccagc ttaaattgtc cgttacggct gtagaacacc
    77041 gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac gtccagacct
    77101 cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc aacttttacc
    77161 cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc tgatttaaag
    77221 ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt ggcggcagcg
    77281 tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt gaaggcttaa
    77341 attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc cagcgtatta
    77401 ccgctgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg ggcaaccagt
    77461 tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg cgcagtgctg
    77521 ccatcagtca acgaaccgtc ccaggtgaaa ctgtgaactc cggcggtcag ttcaccaata
    77581 tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac tttgtctgcc
    77641 gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc ttcactgccg
    77701 gttcccgcaa gaacagttgt gccggggatc atcacgccgt gaccgatcag gttactggcc
    77761 tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt attgagtttt
    77821 tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt gttttccatt
    77881 ggattggtcg ggtcctggtt tttcagctgc gccaccagca aagtcagaaa actgctttgt
    77941 aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac gccggtattt
    78001 gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga ccgagcgtaa
    78061 gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc tgatagctgc
    78121 gtgacgccga catggtgtta accatctccc cgacgacatc aacgttcggc atttttatgt
    78181 agccctttgc atctgccagc ggattacctg gttcataaac cagtttgtcc ggggcctgac
    78241 tttcttaaca tcggcaacct ttacgccgcc tgtcgcagca cctggtgcag cgttaacctg
    78301 gaataccacc tgttttgccc gatatggctg tccatcagga ccggtcacgc tatcagcatt
    78361 tgccagatta ctggccgcca cgttcaggcg ctgggactgg gcagttaacg ccgacccggc
    78421 gatatcaaaa atattcagca gtgccatccg ttaatttccg ctctgtaaaa cgttcatcat
    78481 gcctttgatt tgcccgctca acgcgctaag gctcatctgg tattgcaggc tgttatcggc
    78541 aaactgggtg cgttcgcgat ccatatcgac ggtattaccg tcaagcgaag gctggtccgg
    78601 aatacggtat tgcagttctg cggtaggagg cgtcagcgcc tgcgccggga tgtgctgcgt
    78661 tgaggtcatc gtcagtgcaa ccacactggt tgcatcccgt ccacgttgca tgactttttt
    78721 aagttcactg gcaaaatcga tatcgcgcgc ctgataacca ggggtatcgg cattggcgat
    78781 gtttgctgcc agcacttcct gacgctgggc gcgcagattg agcgcctctt gttgaaaacg
    78841 taaggcggcg tcgagcttat cgagcatatc tcctccgcag gtatcaaaat tctgccatca
    78901 gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg tcgctattta
    78961 tgccgttgat ggtcattgcg gacaggtaca attcacgttg tagaaatggc tgggggcgaa
    79021 aatgctgaca ataaaacgta gcgtggcgat catcgcgata ctgttcagtc cgttaagtac
    79081 ggcgagcaat ctcacatcgc aattgcacaa cttttttagc gcccaactcg cgggggtaag
    79141 tgatgaggtt cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat gcgagcagcc
    79201 attgctttcg atgagcaata attcccgcct gtggggcaat gtgaatgtgt tggcacgctg
    79261 cggtaacgac aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt atgtggttgc
    79321 cgcgatgcct attgtgcggg gaggaaagct ggaagctggc aatgtcaaac tgaaacgtgg
    79381 acgcctggat accctgccac cgcgtacggt gctggatatc aatcaacttg ttgatgccgt
    79441 tagcctgcgc gatctatcac ccgatcaacc tatccagtta acccagtttc gccaggcatg
    79501 gcgggtaaaa gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt ttagcgccaa
    79561 cgcagaaggt caggcgctga acaatgcagc cgtcgcacag aatgcgcggg tgcgcatggt
    79621 atcggaacag gtagtcagcg gcgttgttga tgcagatggg aatattctta taaacctgta
    79681 agctgttaaa gattacccgt cccttgccga taaataagca acacatgata aaagcgccct
    79741 caatgaggaa taaaccatga gtattgatcg cacttcgcct ctgaagcctg taagcaccgt
    79801 tcaaccgcgc gaaaccaccg acgcgccggt aacgaacagc cgggcggcaa aaacaaccgc
    79861 ctccaccagc accagtgtga cgttaagcga cgcgcaagca aaactgatgc aacccggcag
    79921 cagtgatatc aatcttgaac gtgtcgaagc gttaaaactg gcgattcgta acggtgaact
    79981 aaaaatggac accggcaaaa ttgccgatgc gctgatcaac gaagcgcagc aagacttgca
    80041 gagtaactga tcgtatgaca cgtcttgcag agatcctcga ccagatgtcc gctgtgctta
    80101 acgatctcaa aacggtaatg gatcaagagc agcaacatct ctctatgggg cagatcaacg
    80161 gcagccagtt gcaatggatt acagaacaaa aaagctcact gctggcgacg ctggattacc
    80221 tcgaacagtt acgcaagaaa gaacccaata cagcaaatag cgttgatatt agtcaacgct
    80281 ggcaggaaat tactgtgaaa acgcagcaac tacgccaaat gaatcaacat aacggctggt
    80341 tactggaagg acagattgag cgcaatcaac aggcgctgga aatgttgaaa ccgcatcagg
    80401 aaccgacgct atatggggcg aacggtcaga cctcaacacc ccatcgcggc agtaaaaaga
    80461 tttcgatctg aatattatct ccggcctgca ctgcaggccg gaatgcattg ttacaccgtc
    80521 cggcgggcaa attctttaac tttgaagccc agtaccgcca gtgcagcgaa gtacgcggca
    80581 atccccgcca gcacgaccgc cattaaacgc agtaagcgcc agggcatggt acccaatgac
    80641 cactccggca tgatatgtaa catacctaaa agcacgccag acatcaccag taccgccacc
    80701 accagacgca acagaaacgc catccagccg ggttgcggag taaagatttt ctgcttacgc
    80761 aactgccagt aaagcagcga agcattcaga cacgccgcca gaccaataga aagtgacagc
    80821 ccggcatgtt tcaacggacc aataaacgcc aggttcatca attgcgttaa aatcagcgta
    80881 acgatggcaa ttttcactgg cgttttaatg tcctggcggg aataaaagcc aggagccaac
    80941 actttcacta caatcaggcc aatcaaaccc accgagtagg caattaacgc ccgctgggtc
    81001 atcagcgcat caaacgcggt aaatttaccg tactggaaca gcgaaacggt taacggacct
    81061 gaaagaatgc ccaacgcaac cgcactcggc agtgccaaca ggaaacaaag acgcaacccc
    81121 cagtccatca aacggttgta ttcatcatga ttgccactgg caaaactttt cgacagcgac
    81181 ggcagcaaaa tggtgccaag cgccacgccc agcacaccgg agggaaactc cattaagcgg
    81241 tcggcgtaat acatccaaga caccgaaccg gaagcaagaa acgaggcaaa aatggtgttg
    81301 atgattaagg agatctggct cacagagacg ccaaggatcg ccggtcccat ctgtttcacc
    81361 acgcgcattg ctccagcatc gtggaagtta atgcgcggca ggaccagcat gccgatcttc
    81421 ttcaggtgcg gtagctgata caccagctgc agaatgccgc cgaccgtaac agcccacgcc
    81481 agcgccagca ccggtgggtt aaagtacggt gcggcaaaca gcgcgaaacc aatcatgctg
    81541 atgttaagca gtgttggcgc aaacgccgga atcgagaagc ggttccacgt attcagaatc
    81601 gctcccacca gcgacgccag ggagatcagc aagatatagg gaaaggtaat ctttagtagc
    81661 tggctggtca gggcaaattt gtcagctgtg tcagcgaagc ctggcgcggt caccatgatc
    81721 acccacggtg cggcgagcat gccagcgacc gtcacaaccg ccagcgcaag tgtcagcagg
    81781 ccagaaacat aagagacaaa gacccgcgtg gcgtcttcac cctgcttact tttatattcc
    81841 gccagaatcg gtacaaatgc ctgggaaaat gccccttcgg caaagatacg gcgtaacaag
    81901 ttaggaagtt taaaagcgac gaaaaaggcg tcggttgcca tccctgcgcc aaagattctg
    81961 gcgacaattg cgtctcgtgc gaagccaagc acacgcgaaa acatggtcat cgagctgacg
    82021 gcggccagcg attttaataa attcatcggt gttctaatcc ggacccacgg gcaaaacgcc
    82081 tgcaaggcag gcgctgaaag aggcgaatag tctacctaga ttatggtgaa ttgctaccgc
    82141 cagatgttac agggtttatt cactcatcgc atcgcgccag atcttgtcaa cgatacgttg
    82201 cgccagcact gcctattcgc cggcggtttg cggaactgtc tgattttaca cgcattcaat
    82261 gaagtgccgc gcacagccga caaatccacg ttgctcaaga gtgctctgcc aaccaggaat
    82321 cggtttattc actacaccct gcccgcgctc ctcacgccat tcgcgcatat ccgtaatgtc
    82381 gatgagcgca ccgtcagtca cggcctgcac ggtttcacgc tgacttccgg cacggcgatg
    82441 catacaagtg gtgatctgca aaggaccggc cgaaaaatgg tgctcggcaa acagcatttc
    82501 gccagcgtcg ttagtcagta gcgtaccgcc atccagagag gctttgccgc ccgacaacca
    82561 cagcgcggta tccaccacat gcagataatc atccagcaac gtgaaataaa gatcgtgtgg
    82621 cccgacgcta ttgctacgat gtttatccat tcttagcgag gctgcggtgg cgagttgcgt
    82681 ttttaactca ccgtagagtg gtgcgaaacg acggttaaaa ccgaccatca acgtcagttt
    82741 tttccgcgcc gccagttcca ccagcctttc agcatcgcgc agattttctg ccagcggttt
    82801 atcgacacag acatgtaccc ccgcattgag taacgtactg accacgtcaa agtggctggc
    82861 ggtgctggag tgcacaaaaa ccgcatcaca actggcggca aggctggata acgaatcggc
    82921 ataaggaatg cgccagctgt cacaaattgg cagggctttg gcgcgcgtag gcgaccaggc
    82981 tccttgtaac gtccagtcag acgctgccgc cagcaccggt aaccacgctt tttgcacaat
    83041 gccgcctaat cccactacgc cgatacgtaa ttttttcact ttaatctccc agatgggcca
    83101 gcaacgaatc aagacgttgt ttcagttctg ccacttcgat ttccagggct tcgacgcggg
    83161 tctgtaaatc accgtcaacc atgttcgaca tagccgttac cgccggctga tcttcaacct
    83221 caccactgaa aagatgcatg tagcggcttt cgcgtttacc cggttcgcgg gccagacgca
    83281 ccacaaaagg accatcttcg cgattcgcca gttgttccag cgtcgactcc acttccgcca
    83341 tatcgctgaa ttcatacatt cgcgcggcgc ggctgcgcag ttcacccggc gtctgggcac
    83401 cacgcaataa caacgtggtg attaacgcca cttctgcggc gctcagtttc agatcgccaa
    83461 attctgaatt acaaaaacgt tgctcatatt tggtgacccg attaccaaaa ccgctcactg
    83521 tgcgtagata atgacgtttg accagattat ccagctgttc ctgcacttcg gattcactca
    83581 gattcatgac cggttcacgg ttcgttttct gattacaggc cgtgactaca ccattgactg
    83641 agagcgggta ttgctccggc gtggtcacct gcttttccag caagcaacca attacgcgag
    83701 cttccagggc agtaagttga tatttcatga gattctcctt agcggccggg cgtccagtct
    83761 ggggtagtta atgccgtcag tacgtgatcg cgccattgtc catcaatcaa cagatagtct
    83821 ttcgcatagc cttctttttc aaaacccagt cgcgccagta aatcaccgct gcgtttattg
    83881 tgcggcatat aattagccat aatgcgatga atatgttggg tgcgctgcat ataacgaatg
    83941 gctgcggtca gggcttcaaa catgagtcct ttgccctgcc atttttgccc aatcgaataa
    84001 ccgagatagc aggcatgaaa agagccgcga acaacattgg aaaaattggc aacgccaata
    84061 atctcttttt cgtccgggtc gaataagcca aagtagaaag ctgaaccttg tttatgaaat
    84121 tcgttaatca tccctagcct ggcctgccag cctgatggat aacagtggct ttcgtcgcgc
    84181 actggctccc agggcttgag gaaatggcga ttctctgcgt aataatccgc cagacgccag
    84241 gcatcacgat catgcaccag acgcacgacc agtcggtctg tggttaagcg cacttttggc
    84301 acgttactgc gatagccaaa cattctatac ctactccttc ccgtaacgtc tttaatacgc
    84361 tgtaagtatt actatacctg cgcaaccaga ccctgtgaaa agtccaaaat gccattttct
    84421 gcattattca catatttgat aaaaactctc tatcaaagcg acgttttgat aaaaaaatac
    84481 tgtccccgaa gtgatgggaa aagtcttagc ggcaatgcag aatagaaagt gctcatcttt
    84541 tatttcccgg gaggggaaat gtcccgcgta tcgcaggcga ggaacctggg taaatatttc
    84601 ctgctcatcg ataatatgct ggtcgtgctg gggttctttg ttgtcttccc gctgatctct
    84661 atccgcttcg ttgatcaaat gggctgggcc gccgtcatgg tcggtattgc tctcggtcta
    84721 cgccagttta ttcagcaagg tctgggtatt ttcggcggcg caattgccga ccgctttggt
    84781 gccaaaccga tgattgttac cggtatgctg atgcgcgccg ccggattcgc cacaatgggt
    84841 atcgcccacg aaccgtggct attgtggttt tcatgcctgc tctcgggact cggcggcacg
    84901 ttgtttgatc cgccgcgttc ggcgctggtg gtgaaactaa tccgtccaca acaacgtggt
    84961 cgttttttct cgctgttgat gatgcaggac agtgccagtg cggtcattgg cgcattgttg
    85021 gggagctggc tgttgcaata cgactttcgc ctggtctgcg ccacaggggc cgttttgttt
    85081 gtgctatgtg cggcgttcaa tgcgtggttg ttaccggcat ggaaactctc caccgtacgc
    85141 acgcccgttc gcgaaggcat gacccgcgtg atgcgtgaca agcgttttgt cacctatgtc
    85201 ctgacgctgg cgggttacta catgctggct gtacaagtga tgctgatgct gccaattatg
    85261 gtcaacgacg tggctggcgc gccctctgcc gttaaatgga tgtatgccat tgaagcgtgt
    85321 ctgtcgttaa cgttgctcta ccctatcgcc cgctggagtg aaaagcattt tcgtctggaa
    85381 caccggttga tggctgggct gttgataatg tcattaagca tgatgccggt gggcatggtc
    85441 agcggcctac aacaactttt caccctgatt tgtctgtttt atatcgggtc gatcattgcc
    85501 gagcctgcgc gtgaaacctt aagtgcttcg ctggcggacg caagagctcg cggcagctat
    85561 atggggttta gccgtctggg tctggcgatt ggcggcgcta ttggttatat cggtggtggc
    85621 tggctgtttg acctgggcaa atcggcgcac cagccagagc ttccgtggat gatgctggga
    85681 attattggca tcttcacttt ccttgcgctg ggttggcagt ttagtcagaa acgcgccgcg
    85741 cgtcgtttgc tggaacgcga cgcctgattt gatactcccc cctgactcct ccatactgaa
    85801 agtgaactga cagccaaatg gaggagtcac gtgaagctat acatttacga tcactgccct
    85861 tactgcctca aagcccgcat gattttcggc ctgaaaaata tccccgtcga attacatgtt
    85921 ctgctcaacg acgacgcaga aacacccacc cggatggtcg gtcaaaaaca ggttcccatt
    85981 ctgcaaaaag atgacagccg ctatatgcca gaaagcatgg atatcgttca ctatgtcgat
    86041 aaactcgacg gcaaaccgtt actgaccggc aaacgttccc ctgccattga agagtggctg
    86101 cgcaaggtca atggctacgt caacaaactg ctgttgccgc gttttgccaa atcggcattt
    86161 gatgagtttt ctactcccgc cgcgcgcaaa tatttcgtcg acaagaaaga ggccagcgcg
    86221 ggtaattttg ccgacctgct ggcccactct gacggtctga ttaagaatat cagcgatgat
    86281 ttgcgtgcgc tggacaaact gatcgtcaaa ccgaacgccg tgaatggcga actttcggaa
    86341 gatgatattc agctatttcc gctactgcgt aatctgacgc tggtagccgg aatcaactgg
    86401 ccaagccgcg ttgctaatta ccgcgataat atggcgaaac agacacaaat caatttgtta
    86461 tcatcaatgg cgatttaatc ctgacccgcc gcggcagcct gtattcgcgg gttgccgccg
    86521 ttttcagttt tttctgctgg cgagtttatc gctggtgaca catgctgcgt tgtgtcagga
    86581 tattgacgca gatttcatgt cgaggactac catgaacaag tttttatttg ccgctgcatt
    86641 gatcgtcagt ggcctgctcg ttggctgtaa tcaactcacc caatacacca tcaccgaaca
    86701 agaaattaac cagtcgcttg cgaaacataa taatttctca aaagatatcg gtttacccgg
    86761 cgtggctgac gcccatattg ttctgacaaa cttgaccagc caaattggtc gcgaagagcc
    86821 gaataaggtt accctaaccg gagacgccaa tctggacatg aactccctgt tcggtagtca
    86881 gaaagcgacc atgaaactga agctgaaagc gctgcccgtg tttgataaag aaaaaggtgc
    86941 gatcttcctg aaagagatgg aagtggtcga tgcgacggta caaccggaaa aaatgcaaac
    87001 ggtgatgcaa acgttgcttc cctatttgaa ccaggcatta cgcaattact ttaaccagca
    87061 acctgcttac gtcctgcgcg aagatggcag tcagggcgaa gcaatggcga aaaaactggc
    87121 gaaaggcatt gaagtgaagc caggcgaaat tgtcattccg tttacggatt aatgacgagg
    87181 gcgcattcgc gccctttatt tttcgtgcaa aggaaaacgt ttccgcttat cctttgtgtc
    87241 cggcaaaaac atcccttcag ccggagcata gagattaatg actgcaccat cccaggtatt
    87301 aaagatccgc cgcccagacg actggcacct tcacctccgc gatggcgaca tgttaaaaac
    87361 tgtcgtgccg tataccagcg aaatttatgg acgggctatc gtaatgccca atctggctcc
    87421 gcccgtgacc acagttgagg ctgccgtggc gtatcgccaa cgtattcttg acgccgtacc
    87481 tgccgggcac gatttcaccc cattgatgac ctgttattta acagattcgc tggatcctaa
    87541 tgagctggag cgcggattta atgaagccgt gttcaccgct gcaaaacttt acccggcaaa
    87601 cgcaaccact aactccagcc acggcgtgac gtcaattgac gcaatcatgc cggtacttga
    87661 gcgcatggaa aaaatcggta tgccgctact ggtgcatggt gaagtgacac atgcagatat
    87721 cgacattttt gatcgtgaag cgcgctttat agaaagcgtg atggaacctc tgcgccagcg
    87781 cctgactgcg ctgaaagtcg tttttgagca catcaccacc aaagatgctg ccgactatgt
    87841 ccgtgacgga aatgaacggc tggctgccac catcactccg cagcatctga tgtttaaccg
    87901 caaccatatg ctggttggtg gcgtgcgtcc gcacctgtat tgtttaccca tcctcaaacg
    87961 caatattcac caacaggcat tgcgtgaact ggtcgccagc ggttttaatc gagtattcct
    88021 cggtacggat tctgcgccac atgcacgtca tcgcaaagag agcagttgcg gctgcgcggg
    88081 ctgcttcaac gccccaaccg cgctgggcag ttacgctacc gtctttgaag agatgaatgc
    88141 tctgcagcac tttgaagcat tctgttctgt aaacggcccg cagttctatg gcttgccggt
    88201 caacgacaca ttcatcgaac tggtacgtga agagcaacag gttgctgaaa gcatcgcact
    88261 gactgatgac accctggtgc cattcctcgc cggggaaacg gtacgctggt ccgttaaaca
    88321 ataaaaatcg tagcttcctg ttgtcattag gttattttac ctgtataaat aaccagtata
    88381 ttcaacaggg ggctattatg cgaattgaag tcaccatagc gaaaacttct ccattgccag
    88441 ctggggctat tgacgccctg gctggcgaac tttcccgccg tattcagtat gcgtttcctg
    88501 ataatgaagg ccacgtatcg gtacgttatg ccgcagcgaa taatttatcg gttattggcg
    88561 caacaaaaga agataaacag cgcattagcg aaattctcca ggaaacgtgg gaaagcgccg
    88621 atgactggtt tgtcagcgaa taatatgcag tgatttttgt ttttgccgga tcgccccggc
    88681 ttttttattt tacttacgaa ttttgagtac aaaatttcta cacttcctca aaataattgc
    88741 acaatatgct aaaaaaatat gaatgacatg ctgttttatt gttcatataa atcttaaaag
    88801 cagaaattta tctgttcccc cctttaaaaa ttcggtgaat acccttactt attggtatac
    88861 tgaagttgct tcagataaaa acgcattgaa cctcgaataa cgttgtctag taacacgaat
    88921 tagggggcca tgatggaaaa aaataatgaa gtcattcaga ctcatccgct cgtagggtgg
    88981 gacatcagca ccgttgatag ctatgatgcg ctgatgttgc gtttgcacta ccagacccca
    89041 aataagtccg agcaggaagg gactgaagtt ggtcagacgc tctggttaac cactgatgtt
    89101 gccagacaat ttatttcgat attagaagca ggaatcgcca aaattgaatc cggtgatttc
    89161 ccggtaaacg agtatcggcg tcattaacat gacatgacca tccacccaaa aggcacctca
    89221 ccggtgcctt taccatttct gggttgtata tctgcattca gttaattact ctgcttaaac
    89281 catgagagca gagagaaaaa aatgaaatac gatctcatca ttattggcag cggttccgta
    89341 ggcgctgccg ccgggtatta tgcaacccgc gccggtttaa acgtgctaat gaccgacgcc
    89401 catatgccac cgcatcaaca cggcagccac cacggcgata cgcgattaat tcgccatgct
    89461 tatggtgaag gcgaaaagta tgtcccgttg gtcctccgcg cgcaaacgct gtgggatgaa
    89521 ctttcccgcc acaacgaaga tgatcccatt tttgtacgct ctggtgtcat taacctcggc
    89581 ccggctgact ccgcatttct cgccaacgtc gcccacagcg ccgaacagtg gcaactcaac
    89641 gttgaaaagc tcgatgcgca agggattatg gcccgctggc cagaaatacg cgtcccggac
    89701 aactacatcg gcttatttga gactgattcc ggttttttgc gcagcgaact ggcgattaaa
    89761 acctggatcc aactggcgaa ggaagcgggc tgtgcgcaac tgttcaactg cccggtcacc
    89821 gcaattcgtc atgacgatga tggcgtaact attgaaacgg ttgacggtga gtatcaggcg
    89881 aaaaaagcga ttgtctgcgc gggaacatgg gtaaaagacc tgctcccgga gctgcctgtc
    89941 cagcccgtac gtaaagtatt tgcctggtat caggccgatg gccgctatag cgtgaagaat
    90001 aaattcccgg cgtttaccgg tgaactgccc aatggcgatc aatattatgg ttttccggca
    90061 gaaaacgacg cgttgaagat tggcaaacat aacggaggcc aggttatcca ttcagcggat
    90121 gaacgtgttc cgtttgcgga agtggccagt gatgggtcag aagccttccc gttcttgcgc
    90181 aatgtattgc cgggtatcgg ttgctgcctg tacggcgctg cctgcaccta tgataattcg
    90241 cctgacgaag attttattat cgatacccta cccggccacg ataatacact gctcattacc
    90301 ggcctgagtg ggcacggttt taaatttgcg tcagttttag gggaaatagc tgccgatttt
    90361 gcgcaagacc aaaaaagcga ttttgatttg acgccattca ggctttcccg cttccaataa
    90421 tcatcattac ggcctccaga atgaggccgt ttttacagga gtatttaatg cgtcggctgt
    90481 tgcactatct catcaataat attcgcgagc atctgatgct ttatcttttt ctctggggat
    90541 tgttggcaat tatggatctg atctatgtgt tttattttta gttttccgca ctgacataaa
    90601 ctgacaaata tttaatctcc ctttcattta gcgtgaaaaa ttgaatgaga ttattcctgc
    90661 ttctacagca ctcaccagac atctctgatg ctgaacccac taaacttatg aagattcttt
    90721 cattattaat tcaaaattat tgaaataaaa ataaatatca ttcaatttgt tatttttgtg
    90781 ttgcaattca ttctccgaca cgccattttc tttctctcgc aaattcgtat tggatgcact
    90841 tatgtcattc acaaataccc ctgaacgcta tggtgttata tctgcggcct tccattggct
    90901 aagcgcaatt attgtctacg gcatgtttgc tttaggtctg tggatggtca cgctcagtta
    90961 ttacgatggc tggtatcaca aagcacccga actgcataaa agcatcggta ttttgttaat
    91021 gatggggctg gttattcgcg ttctgtggcg tgtcatatct ccaccgccgg gtccgctgcc
    91081 gagttattcg cccatgactc gtcttgccgc aaaagctgga catctcgcat tgtgcctatt
    91141 gctgttcgcc atcggcatca gcggctatct gatctcaact gccgatggta aaccgatcag
    91201 cgtttttggc tggtttgacg tccccgcgac ccttgccgac gctggcgcac aggcagactt
    91261 tgccggtgcc ctgcattttt ggctcgcgtg gagcgtcgta gtactgtctg ttatgcacgg
    91321 atttatggcc ctgaagcatc atttcatcga taaagacgac actctgaagc gcatgctggg
    91381 aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa agcctgcttg gtttaacctt
    91441 cgcgtccctg atgttctctg ccggttcagc ggttgccgcc gattacaaaa ttgacaaaga
    91501 aggtcagcac gcctttgtta acttccgcat acagcatctt ggctatagct ggttatatgg
    91561 cacctttaaa gatttcgacg gtacttttac ctttgatgaa aaaaatccgg ctgccgataa
    91621 agtgaatgtg acaattaaca ccaccagcgt cgatactaac cacgccgaac gcgataaaca
    91681 tcttcgcagt gcggattttc tcaataccac aaaatatcca caggcaacat tcacctccac
    91741 cagcgtgaag aaagacggtg acgaactgga tattaccggc gatctgacgc tgaatggcgt
    91801 aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag ggcgacgacc catggggcgg
    91861 taaacgtgca ggctttgagg ccgaaggcaa aattaagctc aaagacttca atatcaagac
    91921 agatttaggt ccagcttctc aggaagtgga tctgattatt tcagtggaag gcgtacagca
    91981 gaagtaagag tgaaatgttg ccggatggca cgcatccggc aatgatattt attctgttgg
    92041 atcaggaata cccagtgttg tattcagacg tccacgagac ttattaaaga tcttattgcc
    92101 attttcacgt cctgcccgac ggcgtcgctg ttcttctggc ggtaacgcgc tttcttcgca
    92161 gcaaatctca ctacaacaac ctttgtattt ttccgcgcat actggacact gaataaacag
    92221 caggtggcag ccatcatttt tacagttggt atggctgtcg cacggcgcgc cgcactggtg
    92281 gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc cgctcgtcaa aaacaaaatt
    92341 tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc ttacgggcgt attcaataat
    92401 tccgccctcg atatgccaga ctttgttgaa tccgttatgt ttcatccagg cactggcctt
    92461 ttcacaacga atgccgccgg tgcagtacat gacgattttt ttatctttat gtgcctgcat
    92521 catctcgact gcttttggca gctgctcacg gaaggtatct gccggaattt cgagcgcgtt
    92581 ttcaaagtgc cccacttcat actcatagtg gttacgcatg tcgataaata gtgcatcggg
    92641 atcgtcaagc atggcgttca cttccgccgc ttgcagatac tcaccaacat tgctggcatc
    92701 aaagtgagga tcgtcaatac cgtcggcaac aatgcgatcg cgtaccttca tgcgcagtac
    92761 ccagaaggat ttcccgtcat catccaacgc gatattcagg cgtaaaccct ctaaagccgg
    92821 gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg ctcgcaggta cgctgatttg
    92881 cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa acattcagcg cggtaaacag
    92941 ctgatataaa gcgtcacggg tcgccttagg atcggcgatg tggaaatact tgtaaaacga
    93001 aatggtggtt cgcggttcgc tctcagccaa cattttggct tttaacgcgt cgttggaaat
    93061 gcggttgtgt aacactggca tggtgtacgg ttcctgcgag atgggaaagt aaaaatccgc
    93121 ggcatgatat agcaattatc gataattaac atccacatat tttacgctac atttgcgcat
    93181 taaaaattat ttgttattta caagcgcggc aatttcgccc agtcttcagc cacaaatttt
    93241 ggttgcgggc gaaaaaatgc gacaatacat acaattgccc gtataggttg aaaaacagga
    93301 ttgatatgac gaatctaccc aagttctcca ccgcactgct tcatccgcgt tattggttaa
    93361 cctggttggg tattggcgta ctttggttgg tcgtgcaatt gccctacccg attatctacc
    93421 gcctcggttg tggattagga aaactggcgt tacgttttat gaaacgacgc gcaaaaattg
    93481 tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga acaagaacgc cgtaaaatgg
    93541 tggtgaagaa tttcgaatcc gttggcatgg gcctgatgga aaccggtatg gcgtggttct
    93601 ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg catggaacac attcgtgacg
    93661 tgcaggcgca aaaacgcggc atcctgttag ttggcatcca ttttctgaca ctggagctgg
    93721 gcgcgcggca gtttggtatg caggaaccgg ggattggcgt ttaccgcccg aacgataatc
    93781 cactgattga ctggctacaa acctggggcc gtttgcgctc aaataaatcg atgctcgacc
    93841 gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc tggtacgcac
    93901 cagatcatga ttacggcccg cgctcaagcg ttttcgtccc gttatttgcc gttgagcagg
    93961 ctgcgaccac gaccggaacc tggatgctgg cacggatgtc cggcgcatgt ctggtgccct
    94021 tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt gattatgctg ccaccagagt
    94081 gttctccgcc actggatgat gccgaaacta ccgccgcgtg gatgaacaaa gtggtcgaaa
    94141 aatgcatcat gatggcacca gagcagtata tgtggttaca ccgtcgcttt aaaacacgcc
    94201 cggaaggcgt tccttcacgc tattaaatct cccatgccgg atgcttcaga atggcatccg
    94261 gcattaccac agcaaatccc cctgatttag cgataaaagc tctctggatt gcgccccctg
    94321 gaagtcgggc gcataattag tgtgtttatc ttttcttctt atgttcaccg cgcctggcgc
    94381 accaacagcg gattgctatg tcaccctgtg aaaatgacac ccctataaac tggaaacgaa
    94441 acctgatcgt cgcctggcta ggctgttttc ttaccggggc cgccttcagt ctggtaatgc
    94501 ccttcttacc cctctacgtt gagcagcttg gtgttaccgg ccactccgcc ctgaatatgt
    94561 ggtccggtat tgtcttcagt ataatgggtt cctaaacaca tcattttagg tattgaatat
    94621 gagacgggca atcagctaca tcagattttc aagtgaacga caactgaaag gggactctgt
    94681 acgccgacaa tctaagctgg ttactgactg gcttgataag aaccctgagt tttacttgga
    94741 ttcatccttg agctttaaag atttggggaa aagtgctttt agtggtaagc acctgaaagg
    94801 tggtctaggt gactttttaa ccgctatcga gaaagggctt gttaaagctg gtgatacgct
    94861 actaatcgag agtttagacc gtctgtcacg tcaggatatt gatattgctt ctgagctatt
    94921 acgccgtatc ttaagggctg gtgtagatgt agtcacactg agcgatggag aacattacac
    94981 acgagaaagt ttaaaagacc cgttagccct gattaaaagc attctgataa tgcagcgggc
    95041 gcatgaggaa agtttgagaa agtctgagcg tgtccaagct gcatggaacc gtaaaaaaga
    95101 actcatctcc gaaggtatca aggtatctag gcgttgtcct gcatggttaa ggctgaatga
    95161 tgaccgtcga acattcacaa tcattcctga taaagtggaa gttgttaaac gagcatttga
    95221 cctgagatta cagggtttaa gcttttgggc tatcaccaga actttaaacg atgaaggtca
    95281 cttgtcattg aatcaataca cacccaaaca aaaggggtgg tctgatactg ctgtaaagaa
    95341 actgctcaga aatcgtgctg tgattggatg tttcacacct gctggacgtg aagaagttca
    95401 gggttactac cccgcaataa tcagtgagtc actattctat cgagtacagc aactgaacac
    95461 agggcaatat ggtagggctt ctgtatcaag taatccgcta tcagtcaact tgttc
//
LOCUS       DAJTPG010000003        73011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTPG010000003 DAJTPG010000000
VERSION     DAJTPG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 73011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO230_000238"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO230_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5690133.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO230_000239"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO230_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5690134.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO230_000240"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO230_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5690135.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO230_000241"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO230_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5690136.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO230_000242"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO230_000242"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5690137.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO230_000243"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO230_000243"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5690138.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO230_000244"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO230_000244"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5690139.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO230_000245"
     CDS             complement(4598..5320)
                     /locus_tag="OO230_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5690140.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO230_000246"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO230_000246"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5690141.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO230_000247"
     CDS             6520..6738
                     /locus_tag="OO230_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5690142.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO230_000248"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO230_000248"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5690143.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO230_000249"
     CDS             complement(7472..7672)
                     /locus_tag="OO230_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5690144.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO230_000250"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO230_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO230_000251"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO230_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5690145.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO230_000252"
     CDS             10101..12014
                     /locus_tag="OO230_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5690146.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO230_000253"
     CDS             12014..13036
                     /locus_tag="OO230_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5690147.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO230_000254"
     CDS             13030..13248
                     /locus_tag="OO230_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5690148.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO230_000255"
     CDS             13302..14171
                     /locus_tag="OO230_000255"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5690149.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO230_000256"
     CDS             complement(14226..14630)
                     /locus_tag="OO230_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5690150.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO230_000257"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO230_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5690151.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO230_000258"
     CDS             15615..17705
                     /locus_tag="OO230_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5690152.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO230_000259"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO230_000259"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5690153.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO230_000260"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO230_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5690154.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO230_000261"
     CDS             complement(19673..20275)
                     /locus_tag="OO230_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5690155.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO230_000262"
     CDS             complement(20265..20432)
                     /locus_tag="OO230_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5690156.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO230_000263"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO230_000263"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5690157.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO230_000264"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO230_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5690158.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO230_000265"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO230_000265"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5690159.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO230_000266"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO230_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5690160.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO230_000267"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO230_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5690161.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO230_000268"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO230_000268"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5690162.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO230_000269"
     CDS             28269..28436
                     /locus_tag="OO230_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5690163.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO230_000270"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO230_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO230_000271"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO230_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5690164.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO230_000272"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO230_000272"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5690165.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO230_000273"
                     /pseudo
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO230_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO230_000274"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO230_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5690166.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO230_000275"
     CDS             complement(33764..34849)
                     /locus_tag="OO230_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5690167.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO230_000276"
     CDS             complement(34861..36165)
                     /locus_tag="OO230_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5690168.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO230_000277"
     CDS             complement(36177..36530)
                     /locus_tag="OO230_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5690169.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO230_000278"
     CDS             complement(36541..37419)
                     /locus_tag="OO230_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5690170.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO230_000279"
     CDS             complement(37416..38642)
                     /locus_tag="OO230_000279"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5690171.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO230_000280"
     CDS             complement(38643..39806)
                     /locus_tag="OO230_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5690172.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO230_000281"
     CDS             complement(39890..40252)
                     /locus_tag="OO230_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5690173.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO230_000282"
     CDS             complement(40269..41174)
                     /locus_tag="OO230_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690174.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO230_000283"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO230_000283"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5690175.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO230_000284"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO230_000284"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5690176.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO230_000285"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO230_000285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5690177.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO230_000286"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO230_000286"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5690178.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO230_000287"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO230_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5690179.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO230_000288"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO230_000288"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5690180.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO230_000289"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO230_000289"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5690181.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO230_000290"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO230_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5690182.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO230_000291"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO230_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO230_000292"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO230_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5690183.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO230_000293"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO230_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5690184.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO230_000294"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO230_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5690185.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OO230_000295"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OO230_000295"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5690186.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO230_000296"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO230_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5690187.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OO230_000297"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OO230_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5690188.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OO230_000298"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OO230_000298"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5690189.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OO230_000299"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OO230_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5690190.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OO230_000300"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OO230_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5690191.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="OO230_000301"
     CDS             complement(59604..61355)
                     /locus_tag="OO230_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5690192.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="OO230_000302"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="OO230_000302"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5690193.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO230_000303"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO230_000303"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5690194.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO230_000304"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO230_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5690195.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="OO230_000305"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="OO230_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5690196.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="OO230_000306"
     CDS             66301..68622
                     /locus_tag="OO230_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5690197.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="OO230_000307"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="OO230_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5690198.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="OO230_000308"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="OO230_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5690199.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="OO230_000309"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="OO230_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5690200.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..72951
                     /gene="rpnA"
                     /locus_tag="OO230_000310"
     CDS             72064..72951
                     /gene="rpnA"
                     /locus_tag="OO230_000310"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5690201.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17601 a        19778 c        18461 g        17171 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctag gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccgcgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc ctgcttatta
    72661 agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat actgcaaact
    72721 ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc accgaaacac
    72781 aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca atccaaagcc
    72841 ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttgcagacat catgcgattt
    72901 accgggctgt cagaagaaga gttggctgcg gcgagtagac tggccccctg aatctccaga
    72961 caaccagtat cacttaaata agtgatagtc ttaatactag tttttagact a
//
LOCUS       DAJTPG010000004        71465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTPG010000004 DAJTPG010000000
VERSION     DAJTPG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(750..2336)
                     /locus_tag="OO230_000311"
     CDS             complement(750..2336)
                     /locus_tag="OO230_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5690202.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="OO230_000312"
     CDS             complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="OO230_000312"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5690203.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4884..5288)
                     /locus_tag="OO230_000313"
     CDS             complement(4884..5288)
                     /locus_tag="OO230_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5690204.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="OO230_000314"
                     /pseudo
     CDS             complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="OO230_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="OO230_000315"
     CDS             complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="OO230_000315"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5690205.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="OO230_000316"
                     /pseudo
     CDS             complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="OO230_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9325..10095
                     /gene="glcC"
                     /locus_tag="OO230_000317"
     CDS             9325..10095
                     /gene="glcC"
                     /locus_tag="OO230_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5690206.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10219..11360)
                     /locus_tag="OO230_000318"
                     /pseudo
     CDS             complement(10219..11360)
                     /locus_tag="OO230_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11722..13452
                     /locus_tag="OO230_000319"
     CDS             11722..13452
                     /locus_tag="OO230_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5690207.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13449..14363
                     /locus_tag="OO230_000320"
     CDS             13449..14363
                     /locus_tag="OO230_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5690208.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14396..14644
                     /locus_tag="OO230_000321"
     CDS             14396..14644
                     /locus_tag="OO230_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5690209.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14644..15815
                     /locus_tag="OO230_000322"
                     /pseudo
     CDS             14644..15815
                     /locus_tag="OO230_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="OO230_000323"
     CDS             complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="OO230_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5690210.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="OO230_000324"
     CDS             complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="OO230_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5690211.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18028..18588)
                     /locus_tag="OO230_000325"
     CDS             complement(18028..18588)
                     /locus_tag="OO230_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5690212.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18600..19424)
                     /locus_tag="OO230_000326"
     CDS             complement(18600..19424)
                     /locus_tag="OO230_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690213.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19424..20773)
                     /locus_tag="OO230_000327"
     CDS             complement(19424..20773)
                     /locus_tag="OO230_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5690214.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20819..21577)
                     /locus_tag="OO230_000328"
     CDS             complement(20819..21577)
                     /locus_tag="OO230_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690215.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21609..22322)
                     /locus_tag="OO230_000329"
     CDS             complement(21609..22322)
                     /locus_tag="OO230_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5690216.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22496..23188
                     /locus_tag="OO230_000330"
     CDS             22496..23188
                     /locus_tag="OO230_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5690217.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="OO230_000331"
     CDS             complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="OO230_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5690218.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25028..26887)
                     /gene="gss"
                     /locus_tag="OO230_000332"
     CDS             complement(25028..26887)
                     /gene="gss"
                     /locus_tag="OO230_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5690219.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27092..27958
                     /gene="yghU"
                     /locus_tag="OO230_000333"
     CDS             27092..27958
                     /gene="yghU"
                     /locus_tag="OO230_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5690220.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="OO230_000334"
     CDS             complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="OO230_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5690221.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="OO230_000335"
     CDS             complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="OO230_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5690222.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="OO230_000336"
     CDS             complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="OO230_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5690223.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29278..29772)
                     /locus_tag="OO230_000337"
     CDS             complement(29278..29772)
                     /locus_tag="OO230_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5690224.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="OO230_000338"
     CDS             complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="OO230_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5690225.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="OO230_000339"
     CDS             complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="OO230_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5690226.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="OO230_000340"
     CDS             complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="OO230_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5690227.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="OO230_000341"
     CDS             complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="OO230_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5690228.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34936..35223)
                     /locus_tag="OO230_000342"
     CDS             complement(34936..35223)
                     /locus_tag="OO230_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5690229.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35342..36229)
                     /locus_tag="OO230_000343"
     CDS             complement(35342..36229)
                     /locus_tag="OO230_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5690230.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36386..37426
                     /gene="gpr"
                     /locus_tag="OO230_000344"
     CDS             36386..37426
                     /gene="gpr"
                     /locus_tag="OO230_000344"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5690231.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37466..37960)
                     /locus_tag="OO230_000345"
     CDS             complement(37466..37960)
                     /locus_tag="OO230_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5690232.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38151..39035
                     /gene="yghA"
                     /locus_tag="OO230_000346"
     CDS             38151..39035
                     /gene="yghA"
                     /locus_tag="OO230_000346"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5690233.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="OO230_000347"
     CDS             complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="OO230_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5690234.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="OO230_000348"
     CDS             complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="OO230_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5690235.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40725..41912
                     /gene="metC"
                     /locus_tag="OO230_000349"
     CDS             40725..41912
                     /gene="metC"
                     /locus_tag="OO230_000349"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5690236.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42052..42711
                     /locus_tag="OO230_000350"
     CDS             42052..42711
                     /locus_tag="OO230_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5690237.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42751..43650)
                     /gene="yqhC"
                     /locus_tag="OO230_000351"
     CDS             complement(42751..43650)
                     /gene="yqhC"
                     /locus_tag="OO230_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5690238.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43844..45007
                     /gene="yqhD"
                     /locus_tag="OO230_000352"
     CDS             43844..45007
                     /gene="yqhD"
                     /locus_tag="OO230_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5690239.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45112..45939
                     /gene="dkgA"
                     /locus_tag="OO230_000353"
     CDS             45112..45939
                     /gene="dkgA"
                     /locus_tag="OO230_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5690240.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46139..47065
                     /locus_tag="OO230_000354"
     CDS             46139..47065
                     /locus_tag="OO230_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5690241.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47116..47373
                     /gene="yqhH"
                     /locus_tag="OO230_000355"
     CDS             47116..47373
                     /gene="yqhH"
                     /locus_tag="OO230_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5690242.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47416..49635)
                     /locus_tag="OO230_000356"
     CDS             complement(47416..49635)
                     /locus_tag="OO230_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5690243.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="OO230_000357"
     CDS             complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="OO230_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5690244.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="OO230_000358"
                     /gene_synonym="parF"
     CDS             complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="OO230_000358"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5690245.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52204..54462)
                     /gene="parC"
                     /locus_tag="OO230_000359"
     CDS             complement(52204..54462)
                     /gene="parC"
                     /locus_tag="OO230_000359"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5690246.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55008..55490)
                     /locus_tag="OO230_000360"
     CDS             complement(55008..55490)
                     /locus_tag="OO230_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5690247.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="OO230_000361"
     CDS             complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="OO230_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5690248.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56087..56746
                     /gene="qseB"
                     /locus_tag="OO230_000362"
     CDS             56087..56746
                     /gene="qseB"
                     /locus_tag="OO230_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5690249.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56743..58017
                     /gene="qseC"
                     /locus_tag="OO230_000363"
     CDS             56743..58017
                     /gene="qseC"
                     /locus_tag="OO230_000363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5690250.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58063..58395)
                     /locus_tag="OO230_000364"
     CDS             complement(58063..58395)
                     /locus_tag="OO230_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5690251.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58714..59295
                     /gene="mdaB"
                     /locus_tag="OO230_000365"
     CDS             58714..59295
                     /gene="mdaB"
                     /locus_tag="OO230_000365"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5690252.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59326..59640
                     /locus_tag="OO230_000366"
     CDS             59326..59640
                     /locus_tag="OO230_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5690253.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59688..61580)
                     /gene="parE"
                     /locus_tag="OO230_000367"
     CDS             complement(59688..61580)
                     /gene="parE"
                     /locus_tag="OO230_000367"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5690254.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="OO230_000368"
     CDS             complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="OO230_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5690255.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="OO230_000369"
     CDS             complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="OO230_000369"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5690256.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63042..63464)
                     /locus_tag="OO230_000370"
     CDS             complement(63042..63464)
                     /locus_tag="OO230_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5690257.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="OO230_000371"
     CDS             complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="OO230_000371"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5690258.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64299..65780
                     /gene="tolC"
                     /locus_tag="OO230_000372"
     CDS             64299..65780
                     /gene="tolC"
                     /locus_tag="OO230_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5690259.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65928..66599
                     /locus_tag="OO230_000373"
     CDS             65928..66599
                     /locus_tag="OO230_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5690260.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66605..67765
                     /locus_tag="OO230_000374"
     CDS             66605..67765
                     /locus_tag="OO230_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5690261.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67803..68591)
                     /gene="ygiD"
                     /locus_tag="OO230_000375"
     CDS             complement(67803..68591)
                     /gene="ygiD"
                     /locus_tag="OO230_000375"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5690262.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68734..69507
                     /gene="zupT"
                     /locus_tag="OO230_000376"
     CDS             68734..69507
                     /gene="zupT"
                     /locus_tag="OO230_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5690263.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69565..69735)
                     /locus_tag="OO230_000377"
     CDS             complement(69565..69735)
                     /locus_tag="OO230_000377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690264.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="OO230_000378"
     CDS             complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="OO230_000378"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5690265.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70753..70901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71023..71313
                     /gene="ubiK"
                     /locus_tag="OO230_000379"
     CDS             71023..71313
                     /gene="ubiK"
                     /locus_tag="OO230_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5690266.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17832 a        19054 c        17649 g        16930 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaac atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gctggcaaaa atcagactgt gcttcacggt gtagcggaac
      841 agttcagatt ctcggcccac catgcccgtc gcggcgcagg ccacggcgat agattgcggg
      901 gagatcatct tgccagttac gccgccgctg gtatttgctg ccaccagcag agtgtcagag
      961 acgttgattt gctgcgccgt ggtcgattgc agtgaaccaa acagggcgtt tgaagaagtg
     1021 tccgacccgg taaggaatac gcccagccag ccgagaaacg gtgagaaaaa cgggaacatc
     1081 acgcctgtac ctgccagtac cagcgccagc gtggtggaca tgccggaata gttggtgacg
     1141 aaggcgaatg ccagcaccat gccaatcgac aatatcggcc acttcagact aattagcatt
     1201 tcggcaaaga cgccaatccc tttcttgatg cccacgccga ggatgaagat agagataatc
     1261 gccgcaataa aaatagcggt gccgccagcc gagagagggt cgaatttaaa caccgcatcc
     1321 attggcgttg gttgggcgac aatgggtgcc gctttcaaca cttgttgatg caaatgaggg
     1381 atctggaaat taatcaccag tgaataaaac gcgccgcccg gagcaaataa cgctttaaac
     1441 ggcttcatgg tccagatggt gaccagcacc gttaagatta aaaacggtga ccacgcacga
     1501 atgatttgcc ccagactata ttctgaaggc acgggaccgc cagaagatgg cttatttacc
     1561 accatcgcac ctgcggattg ccccatgctg attgccgttt cggtattttt cggccgccag
     1621 actttaagga ataaagcgag tgagacgata ctcaccagcg ccgaagtaat atccggcagt
     1681 tccggaccaa tatagttaga ggtaaagaac tgagtgacag cgaagcttcc cccagcaacc
     1741 agcgccgctg gccacgtctc tttcacccct ttccagccgt ccatcattgc taccagccag
     1801 aacggcacaa gaaccgacag gaacggtaac tgacgtcccg ccattgcgcc aatgtggaac
     1861 ggatcgattc ccgttacctg accggcgacc agaatcggca cgcccaacgc accaaacgcc
     1921 accgtcgcac caaagccagc agctccttcc agcaacgcac caaaggagaa accaatcagt
     1981 aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac
     2041 tgcccgctgg caacggttaa tttatacagg aacaccgccg cgacaataat ccacgcaatt
     2101 ggccataatc cataaataaa gccatagccc gcagcagcaa atgccatatc aatcggcatt
     2161 ttaaaggcga atatcgcaat caggatagat aatataaggg ttattgctcc agcaacatgt
     2221 cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc
     2281 agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg
     2341 ttcagtctct tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg
     2401 actgaactgt aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg
     2461 ctacctctgg cataacaagt atatcgggta agggtttctg ttccgcacac gcaggcgcag
     2521 agtatcgtta agatgtccat attgttgttt taggcccgct agtaatgcgc tacgggtatt
     2581 taatattgtt aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag
     2641 ttactatcat agccccgaca ataaaacttg ccggggcttt tttgacgcta ttaatgactt
     2701 tctttttcgc gtaaacgcca ggcgtgtaat aacggttcgg tataaccgtt tggctgtttc
     2761 acgccgagga agattaaatc gctggcagct ttaaaagcac acgagttagc gaaattcccc
     2821 gccatcggac gataagccgg atcgccagca ttttgctgat caaccacttt cgccatattc
     2881 tccagcgacg cctgcacctg ttctttggtc agaataccgt gacgtaacca gttggcgata
     2941 tgctggctgg agatacgcag cgttgcgcgg tcttccatca gcgccacgtt gtgaatatcc
     3001 ggcacttttg aacaaccaat cccctgctcc acccaacgca ccacgtaccc cagaatcccc
     3061 tgcacgttgt tatccagctc ttgttggatc tcttgcgccg accagttagc gttttcagcc
     3121 accggaatag tcagcagatc gtccagcagc ggttcaaatt cagcactgaa ctcggtctgg
     3181 gcaatgttgg cttgcacgct ctgtacgttg gtatggtggt agtgcagcgc atggagcgta
     3241 gcagcggttg gtgacggaac ccaggctgtg tttgccccgg cacgcagttg gtcgcccttc
     3301 tggctgtaca tgtctgccat caggtccggc attgcccaca tgcctttacc gatttgcgct
     3361 ttaccgcgca gcccacagaa cagaccggaa agcacgttat tacgttcgta ggctttgatc
     3421 cacggcgtcg atttcatctg gtttttacgc agcatcgggc cagcttccat caccgaatgc
     3481 atttcatcgc cggtacggtc gaggaaaccg gtattgatga acgccacgcg gttgcgcgcc
     3541 tgagcgatac agctacgcaa gttcagcgag gtccggcgtt cttcatccat aatgcccatt
     3601 ttcagggtat tcggtgccat accgagcatt gtctcaatgc gggtaaacag tttgttggcg
     3661 aacgccactt cctgcggacc gtgcattttc ggtttcacaa tatagacgct gccagtgcgc
     3721 gagtttttct gcacttttaa atcatagagg gcaatcgcgc cagtcatgac gccatcaaga
     3781 ataccttccg ggatttcatt gccttcgctg tcccaaatca caggaatggt catcaaatga
     3841 cccacgttgc ggataaacag caacgagcgt ccgtgcagag aaatttcaga gccatcggcg
     3901 gcggtgtact gacgatcgtc attcagttta cgtacgattt gccgaccgtt tttctccatt
     3961 ttctcttgca gagtcccctg catcaggccc agcagattgc ggtacagcag gattttatct
     4021 tccgcatcaa ccgccgcgac cgaatcttcg cagtcgagaa tggtactgat agcagcttcg
     4081 acgataacat cgttgatgtg cgccggatcg tctttgccaa tccgcccgtt ggcatcaatt
     4141 tgcagttcaa tatgcaggcc gttatttttc agcaaaatgc aggtcggcgc agcggcatcg
     4201 ccacggtaac cgacaaactg tgctggagta cgtaacgtgg tttctttacc atttttcaac
     4261 tggatgcgta attgtttatc gaccacctta aacgccacca catcctgata gctgccgttt
     4321 tccagcggta gtgattcatc gaggaaacgt cgaacccagg cgataacctg ctcaccgcgc
     4381 tgcggatcgt agccactgac catcgcccct tcctgcggga tgatgtcgct gccgtataac
     4441 gcatcgtaca gtgagcccca gcgagcgttc gccgcgttca gcgcgtagcg ggcgttcatt
     4501 gccggaacca ccagctgtgg ccccgcctga ctggtgattt cgctgtcaat gcccgtggtt
     4561 tccaccgtca cgcgctccgg ttgcggcacc aggtagccca gttcacgaag gaaagattta
     4621 taggccgctt tatcttttac cggccccgga ttgctgcgat gccactcatc aagcgccgcc
     4681 tgaatgcgat cgcgttctgc cagcaactga cgattttctg gtgccagatc gtgaacgatc
     4741 tcatcaacat tgtgccagaa tgcagcagcg tccagttcca cgcccggcaa aacttctcca
     4801 tccacaaaac gtttaaaatt ggcgtcaatg cgtaaacggc cctgggttat ggtttgactc
     4861 attgtttctc tcctcgtttt cgcttatttc gccaacaccg ctgctgccac tttcgcgacc
     4921 tgcgcatcct gtgctccggt taaaccagaa acgcccacgg caccaataat ttgcccatca
     4981 acaacaaccg gtacgccgcc ttccagcgac gtcagtaacg gcgcagtcac gaacgcggta
     5041 cgtccgttgt tcaccatctc ttcatagccc ttagtttcac gacgccccag cgcggcggta
     5101 cgcgctttct cctgggagat gtaagccgca atcggcgcgc aatcgtccat gcgacttaac
     5161 gccagcagat gaccgccgtc atcggcaaca gcaatggaaa cagaccagtt atttttctgc
     5221 gcctcttcct gacctgcggc aataattgca ctcgccattt gctggctaag aatgacttta
     5281 gttttcattt ttttattcct tttcaagggc ttgttctaca atttcaatcc agtgacgcac
     5341 agaggtacga ccggcgctcg ccagatgcgt ctggcaacca atgttggcgg tgacgatcat
     5401 ttccggtttg ccgctttcca gcgcattcat tttgttatcc cgcagctggc gtgccagatc
     5461 gggatgcgtt aacgcatatg ttcccgctga accgcagcac agatggctgt cgggaacgtc
     5521 cgttaaggta aatccaagac gaagtaacac tttttccact tcgccgttca gcttttgcgc
     5581 atgttgtagg gtacacgggc agtggaaggc cagtttttta tcgccgcgaa ttgccagttt
     5641 ttccagcggt tcctcgcgca gaagttcgac taaatcgacc gccagttcac tgacctgacg
     5701 cgctttatcg gcatataacg catcgttttt cagcatctgc ccatactctt tgacgaacgc
     5761 gccgcaaccg ctggcggttt gcaaaattgc ctcggcacct gcttcaatcg cgggccacca
     5821 ggcatcaata ttattgcgcg cccgtgccaa ccctttctcc tgcgcattaa gatgatagtc
     5881 caccgcgcca caacagcctg cttcgttagc tgacatgacg ctgatcccca gacgatccag
     5941 cactcgcgca gttgccgcgt tggtgttggg cgaaagcgta ggctgggcgc agccttccag
     6001 cattaaaacc cgacgcttat ggcgcagcgg cggacgcggt ttagctttca ccgtttcagc
     6061 aggcagtttt gctctgacct gttccggtaa aaacggtcgc agcaccagcc ctacctgcgt
     6121 cagcgcacgg aagaccgccg gacgcggcac tacctggcgc aatccttcgc gcagcattcg
     6181 ctccggcagt gggcgtttca ctttctgctc gacaatatca cgcccgatat ccagcaaatt
     6241 gtgatagcgc acaccagaag gacaggtggt ttcacaattg cggcaagtga ggcagcgatc
     6301 gagatgctcc tgtgttttaa gcgtgacttc gttgccttcc agcacctgtt taatcagata
     6361 gatgcgcccg cgcggcccgt ccagttcatc gcccagaagc tgataggttg ggcaggttgc
     6421 ggtacaaaat ccgcagtgaa cacaggcgca caggatgctg tcggcttcca gcgcgcgttc
     6481 tgccgcatct cttcggttaa tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat
     6541 gcgaccgggg ttaaacacgc cgcaagggtc gagctgctgt ttaagctgct ggtgatagcg
     6601 gaataaagga gccgatagcg gggcaaagcc accatctccg gcactaaagc gggtcgcatg
     6661 accgccagcg ttgcgggcga tgcgatggat ttgattgtca tcggctgtcg atttcagcca
     6721 gcgtaacgcc ccgccccagt cgatcagttg ctcgccgggt aaatccatca tcggcgcatc
     6781 gctgggtaat gaaatgcgcc ataaggtacc tggtaacgag aagaacggca gttgttgttc
     6841 acgcaattgc tgccagaact gacccacaac ctcttcgcca cccagcagtt cacgcgctgc
     6901 ttttaccgat ccttcgccgc cctcaaggcg gatccacaac gcattgtcga agtaacataa
     6961 gccactaatg ggtaatggct ggagttgcca ctcggcgatt tcactcatgg cttcttgcag
     7021 gctgatttcc cgacgcaggc tcagagaggc acgcggtcgc ggtaacactt tcattgagat
     7081 ttcagtgagc acgccaagac aaccgtagct tccggccatt aaccgtgaga gatcgtatcc
     7141 ggcaacgttt ttcatcactt cgccaccaaa acgcagatgt tttccagtgc cggtaatgat
     7201 gcgcgtgccg aggacaaaat cgcggaccga accgctccac gggcgacgcg gccccgccag
     7261 cccgcaggcg accatcccgc cccaggtggc ttcttcacca taatgcggcg gctcgcaggg
     7321 aagcatttgc ccggcgctct ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg
     7381 agcggttatc accagctcgg tcgggtcgta attaacaatg ccgcgatgac aacgcacatc
     7441 cagcgtttgc ccggtgactg ggcgacctaa aaaggctttg ctattgctgc cctgaatcac
     7501 cagcggcgtt ttatcgctaa ttgcctgatt cacctgctcc agcagcgcct gactgtaatc
     7561 acactcgcgt agcatcaaaa gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca
     7621 tgcatggcac caaattcagc acagcggtgt agcgtgggaa tgtttttccc agggttcagc
     7681 aaaccatcgg ggtcaaacgc cgccttgacc gcatggaagg tcgtgatttc atcgctgttg
     7741 aactgggcgc acatttgatt gattttttct cgcccgatgc catgttcccc actgatgctg
     7801 ccgccaactt caacgcagag ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat
     7861 tcaccaggtt cgttggcatc gaaaaggatt aacgggtgca tgttgccatc tccggcatga
     7921 aagacgttgg caacacgtaa atcatattgc tgcgataaac gggcaatgcc ttccagtacg
     7981 ccaggcaggg cgcgacgcgg gatggtgcca tccatgcagt agtaatccgg ggagatacgt
     8041 cctaccgccg ggaacgcatt tttgcgaccg gcccagaaac gtacgcgctc tgcttcgtcc
     8101 tgtgccaaac ggacgtcagt cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag
     8161 tcttcctgta cgtcagactc cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg
     8221 acgggataac cggcatgaat aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc
     8281 tccagcccgc cggggataat gccattggcg atgatgtcac caaccgcaag tccggctttt
     8341 tctaccgagt caaagctggc taacagaacc cgcgccacgg gcggcttcgg cagcagtttt
     8401 accgtcactt cggtggtcac gccgagcata ccttccgatc cggtgaacag cgccagcagg
     8461 tcaaaaccag gtgaatccag cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt
     8521 tgcacttcaa ttttcagcag gttatgtacg gtcagaccat atttcaggca gtggacgcca
     8581 ccggcatttt ccgccacatt gccgccaatt gaacaggcga tttgtgagga aggatccggc
     8641 gcgtaataga gattatgcgg cgcaacggcc taggagatcg ccaggttaca tacgcccggc
     8701 tgcacgcgcg cgcggcgacc aacggggtta atgtcgaggc tctctttaaa gcgcgccatc
     8761 accaacaaca cacctttttc cagcggcagc gcgccaccag aaagcccggt gcctgcacca
     8821 cgggtcacca ccggtacacg caggcgatgg cagacagcca gaatcgctgt cacctgttcc
     8881 atttgcttag gcagaacaac cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac
     8941 tcgtaaggaa tgatctcctc atcggtatgc aggatctcaa gtccagggac atgctcacgc
     9001 agtgccatca gtaccgatgt gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg
     9061 tacaagatgc tcatgagtag gcttcgcttt gttgtgttgt gtggcagctg atttttgcgc
     9121 gctgcttctg tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc
     9181 ggtcagacca ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg
     9241 atgtaacata atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt
     9301 cctacctgtg cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt
     9361 gcagagagta tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc
     9421 tcggaacgtc gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg
     9481 accgtgctgc gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca
     9541 cggcttaatc gggagcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga
     9601 acgctgtacg atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg
     9661 gcaacgctgg gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc
     9721 gccgccagtg agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct
     9781 ttccatctcg ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca
     9841 ttgaccgatc tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca
     9901 cagcaaaaaa agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg
     9961 ctgccgcacg tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc
    10021 cacgatatcg agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac
    10081 aaggtgggaa tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac
    10141 ttaacaggat ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc
    10201 gctaaacagg atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc
    10261 cgccccaatg cgttccagca tgttattcat gcctgtattg gtttcgagga tccatgacat
    10321 ctccagcgca tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag
    10381 cgcaatcacc gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac
    10441 tcgcgcagtt cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg
    10501 aaagagcgat ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cacaataaac
    10561 gcgcagggtg cagaatcaat ctcagcgatg tagatcatat cgtccggcac cagatattta
    10621 agttggtcgc ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc
    10681 tgccagccag agttgaaaat ctcacgcagg atctgcatct cttcggcaaa ccgctgacga
    10741 ttgatgcagc gaatggtcac ctttttgcgc acctgatcca tcagtttttt tagcgccgga
    10801 gagaaagtga gatcggttcg ctgcatccac cacgccagta aatcaatgcc tttgtgataa
    10861 cccagttgtt caatatgcgc ggcataccac ggtttgccgt gtggcatcat cgcacagggt
    10921 ggtgtgtcaa aaccttcaat cagtaatccg ctttcctgat tgatattcag gctgaaaggc
    10981 ccgctgatct tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg
    11041 gcaaaatcct gcggatcatc aatggcgtca atcatgccga aatgaccggt atcttcaccg
    11101 taacgctcac ggtgcaaggt atctatttgc gcggtaatac gcccaactat ctgccccgct
    11161 ttttttgcca cccacgcctg ccagatgata tggtccgtcc ccggattttt cgcagacaaa
    11221 tgctcgttgc gttcaatgaa taaagggggt atccagtttg gatcgtcggg ataaagtgat
    11281 gacggaaaag cgataaatgc cttaaggtca tttttattaa ttactttttc aattttaatc
    11341 atcagcagat cgcattccat atctctgtgg ataacaaata attaatggtg aaaaacaggt
    11401 tgcaacagca acgagcggca tcttaataat caactcaatg aatacaaacg acgaataaat
    11461 agtattaatt aatgattatg ataaggtcag aggctacaca gaaacgcctc aatgcctttt
    11521 gtcaatttaa ggcgaccgga caaggcttct tcttaatata ttcataaatt aaatatcatt
    11581 cataataaaa acaaaaaact taaactcatt atttaaatca atgttcattt tcacaccgat
    11641 gaaaatacta tctcatttat cttttatgcc tatataatcc accgcattat tgtcatttta
    11701 tttttgatat ccaatttttt cgtggtgtat atgtctaata aaatctttac gcattcccta
    11761 cctatgcgct atgccgattt tccaacgctg gttgatgctt tggactacgc cgctctgagt
    11821 agagccggaa tgaattttta tgacagacgt tgccaacttg aagatcaact ggaatatcag
    11881 acattaaaaa cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa
    11941 ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc
    12001 tgtcagtatg ctggcttagt cgccgtcccg ttggcgattc caatgggcgt cggtcagcgg
    12061 gattcctgga gcgccaaact ccagggttta ctggcaagtt gccagcccgc agccattatc
    12121 actggtgatg agtggttgcc actggtcaat gccgcgacgc ataacaaccc cgaattacat
    12181 gttttaagcc acgcttggtt taaggcatta ccggaagccg atgttgcgct ccagcgtcca
    12241 gttccgaacg atatcgccta cctccagtac acctccggca gcacccgttt tccccgtggc
    12301 gtcattatca cccatcgcga agtgatggct aatctacgtg ctataagcca cgatggcatt
    12361 aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga tatgggactg
    12421 gtcggctttc tcctgccccc cgtcgccacg caactttcag tagattattt gcgcactcag
    12481 gattttgcca tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt
    12541 tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc
    12601 gctgaactgg atctttcctg ctggcgcgtc gctggtattg gtgctgagcc gatctccgca
    12661 gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta actttgacaa taaaactttc
    12721 atgccgtgct acggactggc agaaaatgcg ctggctgtca gcttctctga tgaagcctcc
    12781 ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg
    12841 ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa
    12901 catggtattg aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat
    12961 atttgcatct ccggtcccag tctgatgagc ggttactttg gcgaccaggt ttcgcaagac
    13021 gagattgccg cgacgggctg gttagacacc ggcgacctcg gttatctgct ggacggttat
    13081 ctgtatgtca ccggacgcat taaagatctg attattattc gtggccgtaa tatctggccg
    13141 caggatattg aatatatagc ggaacaggaa ccggaaattc attctggcga tgcgattgct
    13201 tttgttaccg cccaggaaaa aatcattttg cagatccagt gtcggatcag cgacgaagaa
    13261 cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc
    13321 gcggctatcg atctgttgcc gccccacagt attccccgaa cgtcctccgg caagcctgcc
    13381 cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa
    13441 tccctggcat aaatcaaact gtcgcggtga cgggcgctac cgggtttatc ggtaaatata
    13501 ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt actgcccgcg
    13561 ctcacgtcaa tgataatctc acctgggtgc gcggttcgct ggaagatact cattcactta
    13621 gcgagctggt tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca
    13681 aagaagagat tttcacccgc tgtaacgttg acggcagcct gcgcctgatg caagcagcaa
    13741 aagagagcgg cttttgccaa cgttttctgt ttatctcttc gctggcggcg cgccatcccg
    13801 agctctcctg gtatgcaaaa tccaaatacg tcgccgagca acgactggca gcaatggcgg
    13861 acgaaattac gctgggcgtt tttcgcccga cagccgtgta tggtcccggc gataaagagt
    13921 taaaaccgct gtttgactgg atgctacgcg gcctgctgcc acgacttggt gcgccagata
    13981 cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg
    14041 ccgaaactgt acagacgcaa acctatgaat tatgcgatgg cgtcgccggt ggctatgact
    14101 ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg ttccgtgcga atggttgaca
    14161 ttcctctgcc ggtactcaca ggccttgcgg atatcagtac cgcgttgagt cgcctggcag
    14221 gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg
    14281 caagtaataa ccgtatttct gaagatatca attggtttcc cgggattagc ctggaacacg
    14341 cattacgcaa cgggctattt tgattgtctt gttttaaagg tgtgttaacg tttctatggt
    14401 aaatcgtgaa atagtaatgg attacatcct ttcctgttta caggatttgg tcgaaaacgg
    14461 tgtggaaatt aagcccgaca gcgatctcgt caacgatctt ggcctggagt cgatcaaagt
    14521 gatggatctc ttaatgatgc tggaagacag attcgatatt tccattccta ttaatattct
    14581 gctggatgtc aaaacccctg cgcaattaat ggaaactcta cttccatggc tggagaataa
    14641 ataatggggc tatacgataa atatgcgcgc cttgctggcg agcgtctgca atttagtgac
    14701 aacgggttaa caccgtttgg cacctgtatt gatgaagtgt attccgccac cgaaggccgg
    14761 atcggcaata aaaaagtgat tctggccggg accaataatt atctcggttt aacctttaat
    14821 catgatgcca tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg caccaccggt
    14881 tctcgtatgg caaacggcag ttatgcgccg cacctggcgt tagagaaaga gattgccgag
    14941 tttttcaatc gccctaccgc tatcgttttt caaccggtta taccgctaat ttaggcgtca
    15001 tcagcgcact tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta
    15061 tttatgacgt ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta
    15121 aagatctgga acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg
    15181 ttgagggcat ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca
    15241 aacgtcgtct cggtggttat ttgattgtcg atgaagcgca ttcgtttggc gtgttaggcg
    15301 caacggggcg cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg
    15361 gtactttcag taaaagtctg gcctcgattg gcggttttgc tgtcgtttcg gaagcgatgg
    15421 aagtgctgcg ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca
    15481 ttgcgaccgt gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc
    15541 tgatggataa cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt
    15601 cacatattag cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct
    15661 ggcgcgagct tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg
    15721 caggcatcac gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg
    15781 ccatcattca ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc
    15841 cacggcgggt ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa
    15901 acgctaccgg taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat
    15961 taaccatgat ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac
    16021 ggctacccat cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca
    16081 tcgccagggt caggatgggt tgccccagtt tttcccacag tgcaaggtgg tattcgctgc
    16141 tgggttgccc ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag
    16201 tattccccgg cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa
    16261 gattatctaa tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat
    16321 gcaggaccca cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa
    16381 gcgagagatc gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca
    16441 gcgatttcac cgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt
    16501 catcgtcctc ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg
    16561 gcgacgccac ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca
    16621 gcgccaccag tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac
    16681 tgtttttcga taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa
    16741 gttcgaccag tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat
    16801 aaccgcccgg gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca
    16861 gcaatagccc tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga
    16921 aaacattcat cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag
    16981 gcccattagc atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt
    17041 agccaccagt gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag
    17101 tggcaataac gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag
    17161 cagcgccatc aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc
    17221 ggcaggaaag gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg
    17281 gctttgatcc agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc
    17341 acgcccctga tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc
    17401 gggtgatggg tcaaccacat caaccgaacc ggcgcggaaa atacgggttc gattggcagt
    17461 agaagtataa atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag
    17521 gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag
    17581 ctccgactgc gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata
    17641 catcgacaga agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta
    17701 caagcgtccc ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat
    17761 ctgccctagc gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc
    17821 aatcagtact ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc
    17881 cgcttcggtc agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac
    17941 gataatcagc accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc
    18001 actaacgccc tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat
    18061 gacatgatct acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc
    18121 tttattcagc accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg
    18181 tccattgccg tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt
    18241 gccaacaaca aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg
    18301 ctgaaaacga tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt
    18361 tttcttcgcc gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg
    18421 tgtgctgttc cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc
    18481 ttcctggata taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc
    18541 cggggctaag agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc
    18601 tatagcggaa aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc
    18661 aacggtgcat aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta
    18721 tctcccatca ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt
    18781 tcaggatcaa gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc
    18841 acatcatgaa attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc
    18901 atttgctttt gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat
    18961 gaatagttca ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa
    19021 tacgacacaa tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg
    19081 gtcgttttgc cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa
    19141 cgcaggtgct tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc
    19201 tcatcgcctt tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg
    19261 agccagaatt gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt
    19321 gcaatagtcg gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca
    19381 aatagacgct cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg
    19441 ctgcgttata aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga
    19501 gcgccacgtc cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca
    19561 gatgacagaa catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga
    19621 gaaaagcgcg ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg
    19681 aaatcacaat tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc
    19741 caaacaccag cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac
    19801 cgattccgcc cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg
    19861 gatctaaacg catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg
    19921 cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga
    19981 tccccaccaa cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg
    20041 ttgaccagac aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt
    20101 tgaaggcgtt atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg
    20161 tgccgccaac cagattcgac acgtaccagg caataacaaa acccacaaaa ccaaagtcaa
    20221 aataccaggc tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag
    20281 caatcaaatc aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg
    20341 aaggaatgag cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg
    20401 aatgggaaag gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga
    20461 tatccagcga gaaggaaaat gagacgacat tgcgaaattg ctgcggatta ttgttggtca
    20521 aggctggcgt tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga
    20581 tcgacttggc gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt
    20641 taccggcaca cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta
    20701 accaggcgct attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt
    20761 taaaacccgc caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt
    20821 aagttaaact tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt
    20881 ccagcacttc gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc
    20941 caatcgtcga catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc
    21001 gggagatggc agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg
    21061 ccattttttg gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg
    21121 cacgttcgac gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga
    21181 agcgatcgct gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg
    21241 ccattcttcg cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg
    21301 ttttcatgct ttgggttttc gaggatttgg ccgccaatcg ccgttccagc cagacgccaa
    21361 ccaacggcaa tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc
    21421 gctcggtttg ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac
    21481 cggtgccatc acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg
    21541 ttgtactatt aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat
    21601 tttggtgatc aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg
    21661 gctggttgcc gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta
    21721 acggcgattt tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt
    21781 tgttcatcaa tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac
    21841 agcttcaact cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc
    21901 aattgcgggc catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg
    21961 agcagatagc cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag
    22021 gaaagcagat agataaccag cgcagtaata ttgccaggcg gagttaaggg cttttcgtgt
    22081 acatgtgccg ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt
    22141 gaaatccatt cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt
    22201 cttgctgcca gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat
    22261 cctacaatgg caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc
    22321 acgaaaaacc cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc
    22381 gttcaacctt attgtgacaa tgactaattg tgacattgac ctgaattacg ctttcataaa
    22441 aacatattaa ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca
    22501 gtcaataaca cctccattaa ttgccgttat tggtagcgat gcttcaggca agtcaacggt
    22561 gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg
    22621 aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt
    22681 acataaaaca attgaacgaa atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc
    22741 ggcgctggta attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc
    22801 ctgtcgtcgt cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg
    22861 cgcttacgat ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct
    22921 ggcaagccag gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat
    22981 caaactcaat gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc
    23041 gctggcgagg aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga
    23101 tatcgatgcc aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga
    23161 ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg
    23221 ggcattttca ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa
    23281 aaattgccgc cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct
    23341 gtaacccacc gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg
    23401 tggagacagg catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg
    23461 ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg
    23521 ctacacgacg ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga
    23581 tccacaccgg agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt
    23641 tctggtcttc tttactgacg cctggcagtt tcgccagctt cgcggaggta tcggagatgc
    23701 acagcataat gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca
    23761 tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac
    23821 agtgaaactc tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg
    23881 caatcaactt ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg
    23941 catcgcgggt acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag
    24001 gggcaatccc caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcgcgc
    24061 cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg
    24121 gtttacgttt gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct
    24181 ttttcgtccc actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc
    24241 cgacgatagg ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg
    24301 catccatcac cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac
    24361 caatcaaggt gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga
    24421 taatcgccgc cagcagcatg gaaaagacca tcgccaaacc gtgagttgac cccatattca
    24481 gcaacaaatc gattggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata
    24541 acacgccaaa aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac
    24601 gagtataaat aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt
    24661 agaacaacac aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa
    24721 caaataaatt tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc
    24781 agtgacttta acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta
    24841 tttcttgtgt atcagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag
    24901 gtattccact ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat
    24961 tgaatatttt gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca
    25021 acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca
    25081 gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata
    25141 cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct
    25201 gctcggcaaa tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga
    25261 tattgctacc acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat
    25321 cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga
    25381 tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt
    25441 ccgggcgcag caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg
    25501 gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg
    25561 cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag
    25621 catcccagcc cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca
    25681 gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat
    25741 ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt
    25801 ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc
    25861 ccgcttcggt atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct
    25921 catccatgca gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga
    25981 gacgcaaacg tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt
    26041 tcagcacctt gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta
    26101 gctcctgctc ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct
    26161 gaccgttggc ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt
    26221 caaactggcc tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt
    26281 ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg
    26341 tgtcatcaaa agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc
    26401 gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga
    26461 ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc
    26521 ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg
    26581 cctgcaatgg caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct
    26641 cccacgccat acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag
    26701 caaattcaac gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata
    26761 cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta
    26821 ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt
    26881 tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg
    26941 attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag
    27001 cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc
    27061 ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa
    27121 agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc
    27181 tggtccgacg catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct
    27241 gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt
    27301 tactggtgca gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag
    27361 cggctttgtc gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa
    27421 tccgccgatc cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg
    27481 ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt
    27541 acagggtgcc gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt
    27601 gaaaattgag tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct
    27661 ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat
    27721 ggcgatttgg ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt
    27781 tctggatgcg ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc
    27841 ggcagtgaaa cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca
    27901 tgagcgccat gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg
    27961 gttggtgttc gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca
    28021 atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca
    28081 tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc
    28141 atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag
    28201 atcggcaggg ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac
    28261 ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc
    28321 aatacacatt gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta
    28381 gcgggtgttc cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc
    28441 tttttaggcg ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc
    28501 cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa
    28561 tctccaccac ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta
    28621 aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc
    28681 tctcctcaac gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat
    28741 cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat
    28801 gcattttcgc ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc
    28861 ggattggtga ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct
    28921 tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa
    28981 acaccgtcca gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt
    29041 ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc
    29101 agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa
    29161 acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt
    29221 tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca
    29281 gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac
    29341 ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc
    29401 cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg
    29461 cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa
    29521 cgccggaact tcttcatccc gcaggatcat catcgttccc ggagtgttct ttttcgacac
    29581 aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc
    29641 cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc
    29701 ttcgacaatc cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac
    29761 taaaatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg
    29821 tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt
    29881 ttattcggat cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca
    29941 ttgaagttac gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg
    30001 ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca
    30061 acacctttga actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct
    30121 ttgccgatat tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag
    30181 cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc
    30241 gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat
    30301 tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg
    30361 ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa
    30421 gccggaatgg tggtaccttc ccacggcgcc tgtggcgctt cgtctttata ccaggagtgc
    30481 ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga
    30541 tacgaggaca gatccgcatt ctcaatgtag cctcccggga acaggaagct gccgtttttg
    30601 ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc
    30661 cattccgggt agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag
    30721 tcgctcagtt tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc
    30781 aacccgtcga ggttgattgg gttcgcgaca ccacccacag ccaggttctg aatgtgcggc
    30841 gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc
    30901 aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc
    30961 cagtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc
    31021 tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt
    31081 tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc
    31141 gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta
    31201 cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg
    31261 gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca
    31321 tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc
    31381 catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata
    31441 cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat
    31501 gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc
    31561 aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg
    31621 cgtaaccgcc gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac
    31681 ctaacagtgc gctcagtgct gacaggaaca gcatacggga atcattacgc agcttcgcca
    31741 cacgcagaac gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt
    31801 agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca
    31861 gcccgatgaa aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct
    31921 tttcatccgg accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa
    31981 tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct
    32041 gccagagcgg atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt
    32101 gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt
    32161 gtagcgacac cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca
    32221 cgccgatgta gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt
    32281 gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca
    32341 agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt
    32401 attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc
    32461 cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt
    32521 cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga
    32581 ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat
    32641 catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc
    32701 acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga
    32761 atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca
    32821 taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga
    32881 tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc
    32941 ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc
    33001 tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga
    33061 ccgcctttat cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca
    33121 ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa
    33181 cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc
    33241 agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc
    33301 ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac
    33361 acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc
    33421 ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag
    33481 cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt
    33541 tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct
    33601 gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct
    33661 tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca
    33721 caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat
    33781 ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat
    33841 ggaagccgat acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca
    33901 caccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac
    33961 agccgaggtg gtacaagcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa
    34021 aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg
    34081 cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg
    34141 caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg ataacggttt
    34201 tgcctggcag aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa
    34261 caccgcccca cgcggagcag gaaccaatag cgataatggc tgctgagcct tccgccgctt
    34321 tgcggatgtg atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta
    34381 atgggatgga accatccacc actaacacat actgcccttt gtacttctcg agagcgttat
    34441 gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag
    34501 agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg
    34561 tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa
    34621 ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg
    34681 cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc
    34741 cagtcattta tagttattcc gttgcgagaa cctggcatat attttgcctc aatcgcaaaa
    34801 tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat
    34861 actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat
    34921 aatacgtatg tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga
    34981 acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg
    35041 cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatcct
    35101 gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac
    35161 cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt
    35221 catcgttcac ctcattcatt atatatcgcc atatataacg atattttagg caaagaacga
    35281 cttttgcgca cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct
    35341 atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag
    35401 atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat
    35461 aagcctcata aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt
    35521 tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat
    35581 cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga
    35641 cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg
    35701 tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg
    35761 ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa
    35821 cggaacttaa tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca
    35881 catcttcgat atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt
    35941 tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg
    36001 aaggatacgt gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca
    36061 gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat
    36121 acttcgccgc aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt
    36181 agtaatccaa caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc
    36241 tcaactggtg tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact
    36301 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
    36361 aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
    36421 gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcattat cgctcggttt
    36481 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc
    36541 gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
    36601 cgcagaagag aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact
    36661 gattatctct accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc
    36721 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
    36781 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
    36841 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
    36901 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
    36961 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
    37021 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
    37081 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
    37141 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
    37201 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
    37261 tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt
    37321 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
    37381 cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg
    37441 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
    37501 ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt
    37561 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
    37621 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccacttca
    37681 gcttctcttt gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca
    37741 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
    37801 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
    37861 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
    37921 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
    37981 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
    38041 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
    38101 atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt
    38161 taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
    38221 caagcatcca ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta
    38281 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
    38341 ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
    38401 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
    38461 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
    38521 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
    38581 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
    38641 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt gtcatcacca
    38701 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
    38761 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
    38821 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
    38881 aggataagat cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg
    38941 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
    39001 tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
    39061 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca
    39121 aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc
    39181 aggcattgag cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac
    39241 gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat
    39301 tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc
    39361 ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg
    39421 gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt
    39481 ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata
    39541 aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac
    39601 atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc
    39661 gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag
    39721 atgcattgcc atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc
    39781 agcggcgctg gcttccagat ccaggtcacg gctttgcagc aacaatacct gcgctgcaac
    39841 atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac
    39901 cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg
    39961 cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat
    40021 gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata
    40081 gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct
    40141 ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg
    40201 cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt
    40261 ggcctggttt aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt
    40321 ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta cagaggccaa
    40381 aataagccca atcatcacgc acttaacgac aatatcggcg tactgataca taccccagac
    40441 ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa
    40501 aatctgcgca taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg
    40561 catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca
    40621 cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc
    40681 atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc
    40741 ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata
    40801 gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga
    40861 cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt
    40921 ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg
    40981 gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga
    41041 tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg
    41101 gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc
    41161 caaacactaa aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg
    41221 ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca
    41281 acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc
    41341 aggccgccac caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca
    41401 atgcccgttg ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg
    41461 ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac
    41521 atcatgaaag cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgcgcgag
    41581 ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag
    41641 gcagcagcgg gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga
    41701 actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt
    41761 tgatcctggc aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt
    41821 ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg
    41881 atctggacgc cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga
    41941 cattttattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc
    42001 aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt
    42061 attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat
    42121 attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg
    42181 cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa
    42241 ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc
    42301 tggctaagtt atattcaggg gagctggtta gggaatacca aaacggtgaa aggctggctg
    42361 gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg
    42421 ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg
    42481 ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg
    42541 tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc
    42601 catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc
    42661 ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc
    42721 ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc
    42781 cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc
    42841 gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat
    42901 catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac
    42961 agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc
    43021 gacgctcagg ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact
    43081 gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac
    43141 gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc
    43201 catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt
    43261 aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag
    43321 ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt
    43381 ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc
    43441 ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat
    43501 gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac
    43561 atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc
    43621 cagcaagcga caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca
    43681 atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat
    43741 tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag
    43801 atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct
    43861 gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat
    43921 tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt
    43981 tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga
    44041 gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt
    44101 gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc
    44161 agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga
    44221 gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc
    44281 caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt tccattctgc
    44341 acatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg
    44401 tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa
    44461 accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctaatcga
    44521 agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg
    44581 ggcggcgact caggcgctga acggtttgat tggcactggc gtaccgcagg actgggcaac
    44641 gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc
    44701 tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta agctgctgca
    44761 atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc
    44821 gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta
    44881 cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac
    44941 ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc
    45001 ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc
    45061 acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat
    45121 ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg
    45181 caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt gggttatcgc
    45241 tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat
    45301 gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag
    45361 cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc
    45421 tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg
    45481 atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat
    45541 cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt
    45601 catccgctga tgcaacaacg ccagctacac gcttggaacg cgacacacaa aatccagacc
    45661 gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc
    45721 gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat
    45781 agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat
    45841 gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc
    45901 aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc tcccggtggc
    45961 ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt
    46021 gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc
    46081 tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat
    46141 gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc
    46201 ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag
    46261 tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc
    46321 gcttaccctg aacgacaatc ttacactacc gggcaatatt ggctggctgg attatgatcc
    46381 ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga taacccaaac
    46441 tgacgtcgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa
    46501 gaccacacag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg atgatattgt
    46561 cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc
    46621 tttagcttcg ttgcagaaag aacagccaga atcctttgtt gctgaactct ttgaacatat
    46681 tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa
    46741 tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga
    46801 acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt
    46861 tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac
    46921 gctgaccaat ggcaacaatg aagagcaaga gctggtttta cagcggcgcg aaggtaagtg
    46981 ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa
    47041 aaccgccgcc agattaaagc aatgagctga attaaataac aattagccgg aacaataaat
    47101 aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc
    47161 ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca
    47221 ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat
    47281 gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat
    47341 tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt
    47401 tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg caccagcatt
    47461 caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct gacgctgagt
    47521 cgccatcggc gtatgtttag tcaacgccgg acgggtattg cggttctggc gacgagcttc
    47581 acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc caatcagatg
    47641 ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg gatcgtggta
    47701 acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga agacgccttc
    47761 gctcttataa ccaatcttcg ccagcggttt tttcccggtg taatacatgg tggttgagtt
    47821 cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat gctttttcag
    47881 ccacagcgcc aaattcacca tatcttcatc acgcgtaccg gggtgcgcag agatgaaata
    47941 cgggatcaga tactgctctt tacctgcctg tttcgagtag gtatcgaaca gctctttaaa
    48001 gcggtcatag ctgcccatgc ccggcttcat catcttcgac aacggccctt cttcggtatg
    48061 ttccggagca atcttcagat aaccgccgac gtgatgggtc gccaactctt tgatatagcg
    48121 cggatcttcc acggcgatgt cataacgtac gcctgaggcg ataaggattt ttttaatgcc
    48181 tttcagatca cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag tgtccatgtg
    48241 cggacaaata tccggataaa cgcacgacaa acggcgacaa gtttgttcag cgcgtggcga
    48301 tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa tcacgcccgt
    48361 aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat cttccgaacg
    48421 gctctgaata atgcgccctt cgtgctcggt aatagaacag aaagaacagc cgccaaagca
    48481 gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac gggcattgcc
    48541 ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca tctcttcggt
    48601 agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt tttgcatcaa
    48661 tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat gggcgtacag
    48721 cactttatcg cccttcactt tctcgaaaga aggcagcaat acgtaggttt tttcccacgg
    48781 tttctggcgc ggtggctgca cgattacagc tttcgcttcc tgctttttcg gcgcaacagg
    48841 tttgttatcc gcacacggca aatcttcgcc atacggatgc gggattgggt cgattttccc
    48901 tagggtatca agacgggtgg aatccacacc gctccagcca ggcagcgcct cttttacgat
    48961 aattgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg ccagacgatg
    49021 cgctacttcc accagcggac gttcaccgtt accaaacatc agcatgtcgg ctttcgaatc
    49081 taccagaacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg tacggcgcag
    49141 gctagcctca ataccgccga ggatcaccgg cacatctttc cacgcctctt tacaacgctg
    49201 ggtataaacc agcgtggcgc gatccggtcg cttacccgcg acattatctg gcgtgtaggc
    49261 atcatcatga cgtaaacggc gatcggcggt atagcggttg atcatcgaat ccatgttgcc
    49321 agcagtaaca ccaaaaaaca gattcggttt acccagacgc ataaagtcgt ctttgctgct
    49381 ccagtccggc tgggcgatga tcccgacgcg aaagccctgc gcttccagca tacgaccgca
    49441 aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc
    49501 gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa acggtgccgg
    49561 tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg gttggatcag
    49621 ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg ccggggatta
    49681 taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc gggcgagtat
    49741 gaagattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc cgccatttcc
    49801 agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca
    49861 agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac
    49921 ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg gaacgcttgc
    49981 ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt gacatcaata
    50041 cggttgacgt cccacagctg tccattaata cccgggtcat cacccagact gatatcgcga
    50101 ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg aagactgtct
    50161 gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccaaggtaga aaccagaata
    50221 ctggatggct caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc accacaggtg
    50281 atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc gcccggtgcc
    50341 agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc agaaatcaca
    50401 tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga cgcgttcagc
    50461 agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc
    50521 agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag
    50581 ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata atggttgggg
    50641 ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag gccgttatag
    50701 acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg
    50761 cgaataggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc
    50821 atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg
    50881 ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa
    50941 cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt
    51001 tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc
    51061 ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca aagtgcaatc
    51121 cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc
    51181 taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt
    51241 ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta
    51301 tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga ctgacgtcaa
    51361 ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga ttaatcttat
    51421 tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa
    51481 atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct tccgggaaca
    51541 tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg gtgccgtgag
    51601 ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga
    51661 agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt
    51721 tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata gcattgccgt
    51781 agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagt ggcgcaagac
    51841 ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt
    51901 aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga
    51961 tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca cctgaaatca
    52021 ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga
    52081 ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg ctgccggatg
    52141 acgacttaac gtttcatccg gcgttccttg ctagcgggag gaaacagcgc cctccccggc
    52201 atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct caacacgatc
    52261 gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag tgactttctg
    52321 tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca gcgtgctttg
    52381 cggcggcaga acgtacagtt gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg
    52441 aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa
    52501 catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat cttcaatcac
    52561 caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac ccgcacggtt
    52621 acgcgctacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag
    52681 cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag gcggcaacgt
    52741 taatttgccg gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat
    52801 ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttactct tacctttcac
    52861 cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga tatcatggcc
    52921 tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt cagacggcag
    52981 catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc
    53041 gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat tcattttacg
    53101 ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac tctgctcacc
    53161 gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga ggatcgcttc
    53221 cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt catcttcgtt
    53281 acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac cttccaggat
    53341 atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca cggtatcgcg
    53401 acggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg gacgaccatc
    53461 cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg tagcgaagag
    53521 gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca ccagacgggt
    53581 cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct ttttgttgcg
    53641 catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg gtaatgcgct
    53701 gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg aaccacgtcc
    53761 gttctcgtag attttacgaa tctcggcgcg cgaagtgata atttccgctt cagtcggata
    53821 atccggcccc tgcacgatat ccagcagctg atcgagcgta gttttcggct ggtcgattaa
    53881 tgcgattgcc gcctgagcta cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc
    53941 gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag gtagcatttt
    54001 cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct gccccagctc
    54061 gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa
    54121 cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg gataacggta
    54181 agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgctatcgc cgtgcggatg
    54241 gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg cgctggcatt
    54301 caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttca gaccatcacc
    54361 aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt aggcgttttc
    54421 cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg attcctcaac
    54481 ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc tgccgcgtcg
    54541 agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg ataaaggcat
    54601 caatgtgcct tgatatacaa ctctttgctg tagacttgat cggcatagag ggcagcacaa
    54661 ggttgcgccc atgccacacc actcggcatg ggggtccgct ccgggcgatt cgagaagttg
    54721 aggttatgcg agtccaggga agcctaaagc atcaaagtag tcgactgtaa gtatacggtt
    54781 cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct cctgccataa gtgcggcttg
    54841 ctcatggata tccccgagac ggcttacggc gcaccggata ataaatttcg cgtatacatg
    54901 gtattcctta ccggagaaat tataagccca acaacaatac gcctgtgaca ttgttcgctc
    54961 aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttccccatca cgccaacggc
    55021 acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt ggtgtagtga
    55081 aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg gcgaataatg
    55141 ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac ggcataacgt
    55201 ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcag ttcgctaacg
    55261 ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcaggggc gtcccaggcg
    55321 acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata
    55381 aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca
    55441 gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat tttgcctccc
    55501 tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat ttactttgcg
    55561 gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc
    55621 aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt ttgtgatcga tatcaacatt
    55681 gatagtaccg ctggcatctt taaacacgta gagatcgtca gagatgcgtt caacgatatt
    55741 gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta cagtcgttac
    55801 gctgccgttc ggcccctgaa atcctccggc ctgactttgc gttaccgatg ggccagaaaa
    55861 accgccctgc tctgctgcca tcaccggtgc actgcacagg gccattactg cgattactgc
    55921 tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat
    55981 agtccttaac aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac
    56041 aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact
    56101 gatagaagat gacatgctga ttggtgacgg catcaaaact ggccttagta aaatgggttt
    56161 tagtgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata gcgcacctta
    56221 tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata ttttgcgcga
    56281 atggcgagag aaaggtcagc gtgagccggt actgaacctg accgcgcgtg atgcgttagc
    56341 ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt
    56401 gatagaagtc gccgccaggc tggaggctct gatgcgccga accaacggcc aggccagcaa
    56461 cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg
    56521 cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga tgcgtaacgc
    56581 tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt
    56641 caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt
    56701 tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt
    56761 cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt gacctggctg
    56821 ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt gttcgacacc
    56881 caactgatgc tgtttgccaa acggttaagt acgctcgatc tcaacgaaat caacgcggcg
    56941 gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga cgatgcgctg
    57001 acctttgcca tctttaccca cgacggcaga atggtcctta atgatggcga taacggagaa
    57061 gatattccct atagctatca acgggaaggt tttgctgacg ggcaactggt cggtgaagac
    57121 gatccttggc gttttgtctg gatgacctca cctgatggca aatatcgcat cgttgttggc
    57181 caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca attgatcccg
    57241 tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc
    57301 gcgccgctga acaaactggc gctggcacta cgtatgcgtg accctgactc ggaaaaacca
    57361 ctaaacgcga ctggcgtacc cagcgaagtg cggccactgg ttgagtcgct aaatcaactg
    57421 ttcgcccgca cacatgcgat gatggttcga gaacgacgct ttacctccga tgcagctcac
    57481 gaactacgta gcccgttaac agcgctgaaa gtgcaaaccg aagttgcgca gctctctgac
    57541 gatgatccgc aggcgcggaa aaaagcactg ctccaattac attccgggat cgatcgcgct
    57601 actcgtctgg ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag
    57661 gacgtcgcgg agatcccgct tgaagatctc ctacaatcgt cggtgatgga tatttaccac
    57721 acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgcccacgg catcaaacgc
    57781 accggacaac cgctattgct aagtttgttg gtgcgaaatt tgctggataa cggccccggt
    57841 gtgacaccag aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc
    57901 gctaccggca gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatgacatg
    57961 aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt
    58021 ctctgtctat gagagccgtt aaaacgactc tcatagattt tactaatagc aaaatataaa
    58081 ccgtccccaa aaaagccacc aaccacaaac cagacaaata acgagcgaga atacatatag
    58141 ccaaaactcg aacatttccc gcataaagag ttttcttaag ataagaataa taagtggcgt
    58201 aagaagaaaa aatgctgcaa tcattgcttg caatatacga tcttcgcctg aaacggaagg
    58261 tatactgcaa atattgcctc ccgactcaag catccagtca tacttattga aagcaaaaaa
    58321 agaatctatt aaccaataag taagcattat atatagcaat gcttcaaata ttgttttaat
    58381 tttctgcttt aacatatatc agtacgctca taaaaatcca tttataatta tcaacatgac
    58441 acgttgatat tttcatcttt tacttattca aagccatatt ttcaatcccc aaaaaaaccg
    58501 aaccaaacgt gtaaattatt ccggtgcgag taagaattat gaggaatggc tatcagtatt
    58561 gtcattttca gaaaatattt atcctgcatc ggtgagtcag agctagatca gacttttgct
    58621 aaattcgcaa aagactttgc acattttgct aatttcacag taccgctctg tgacgtacta
    58681 tagtcgacaa acgtctcacc ttgagattaa aaaatgagca acatcctgat tatcaacggc
    58741 gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga agtcgcggat
    58801 ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag cgactacaat
    58861 gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca gatgccaggc
    58921 tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt caccgaaggt
    58981 cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccgtcgaa aaaatacggt
    59041 tccggcagcc tggtacagga caaaaaatat atgctttctc taacctggaa cgcaccaatg
    59101 gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gcgttgacgg tgtgtatctg
    59161 ccgttccata aagcaaacca attcctcggt atggaaccgc tgccgacatt tatcgctaat
    59221 gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa gcatcttgtg
    59281 gaaatttttg gttaactaga gctcaggctt tagaaggagt taaccatgct taccgtaatc
    59341 gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cggtattgga tcagtttgct
    59401 aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc aatggtggat
    59461 tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat gattgagcag
    59521 tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc cgcacatgaa ggcgtacagc
    59581 gaagccgtaa aaggcgacgt gctggagatg aatatccgta ttctgcagcc agggatttaa
    59641 tcctgccttg tttgcccggc catcctgacc gggcaatgtt ctttccttta aacctcaatc
    59701 tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc
    59761 agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc
    59821 accagacggc gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg gttcatttcc
    59881 cccagacctt taaaacgctg gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt
    59941 acgccctctt tctcttcttc cgtcagcgcg taataaacct ctttcccgag atcaatacgg
    60001 tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg gaagtgtttt
    60061 acgaacaaag cgcagagcag cgtggcaatg tgcagaccat cagagtccgc atccgcgagg
    60121 atacagattt tgccataacg aagctggctc agatcgtcgc tgtcaggatc gataccgatc
    60181 gctaccgaaa tatcatgcac ttcctgcgag gccagcactt cgtcggaaga aacttcccag
    60241 gtgttaagga tcttaccttt cagcggcatg atcgcctgat attcacgatc gcgcgcctgc
    60301 ttggcagatc cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg gttaaggccc
    60361 tgcgcggtac aatcagccag tttgccaggc agcgccgggc cgctggtcag ctttttacgc
    60421 accacttttt tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccagc
    60481 aactccgccg cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc
    60541 gaaacgaatg ccgcacactg acgcgaagag agacgctctt tggtctgccc ggcaaactgc
    60601 ggatcctgca ttttaaccga cagaacatag gcgcagcgat cccagatatc ttccgccgac
    60661 agcttcacgc cgcgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccaac
    60721 aggccctgac gcagaccatt aacatgggta ccgccctgca tcgttgggat aaggttgacg
    60781 tagctttcgg tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccacagct
    60841 tcagtatcgc cagcgaaatt accgataaac ggtttttctg gcagcgtcgg taaaccgttt
    60901 accgcttccg ccaggtaatc attcagaccg tcctgatagc accagcgctg ttcggtgttg
    60961 ttgatctcat ctttaaaggt aatctcaacg ccggggcaca gtactgcttt ggccttcagc
    61021 acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag
    61081 aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc cgacaacctg taaatcctgc
    61141 accttttcgc cattttcaaa ggcgatgtta taaacctgac catcgcggcg cacgttaact
    61201 tctacgcgct tcgacaaggc gttaactacc gaaatcccca cgccatgcag gccgccagag
    61261 aactggtaat ttttgttaga gaatttaccg cctgcatgca gacggcaaag aatcagttca
    61321 accgccggta ctccttcttc cgggtgaata tccaccggca tcccgcgccc atcgtcaatc
    61381 acttctaacg actggtcagc atgtaagatt acatccacgc gttttgcgtg acccgccagt
    61441 gcttcatcga cactgttatc aataacttct tgccccaaat ggttagggcg agtggtatcg
    61501 gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca
    61561 gcgttataag tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc agagatggtg
    61621 cagaccaaga aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt
    61681 gccgccttct atgacagtct ggcggcagga agcatagtac gccaccgcct ggcggtaatc
    61741 cagcacttca tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc
    61801 aatctgcatg actttgagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt
    61861 gtaggggttc tcgttctgac cgagatagtc cgtcagcaat tcaaacgggc gtaccgccgg
    61921 gtttaccacc actgcgggca gcataaaaca ttgcgacaac caggtggcgt aatatccccc
    61981 cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag
    62041 cagttctgcc gcgtcggaag gatacggcgg caactgcgga atgatcatct caacgtcagg
    62101 gtgatgttcc gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt
    62161 gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg
    62221 aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactcg
    62281 agagtacgcc agccgggcgc gatagtatcc agcgtaaagt tggaacagtg cggcttaaac
    62341 tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc
    62401 tgatgaatat gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc
    62461 agttcgcccg cgttacgcag actgtgttga tcgagccaac tacaacccgc aggtagcgga
    62521 tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc
    62581 cactcaagct gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc
    62641 aacaggattt gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc
    62701 tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca gacgcagggc
    62761 gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc
    62821 gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc
    62881 agcaccgcct ggtagctttc ccaggtgttt acccctaaca gggcttcgtg cttttgtgca
    62941 aacaggtgag tatcggtaat ttgtaaaatc cttcctctgg cctcaccagc cagaggaagg
    63001 gttaacaggc tttccaaatg gtgtccttag gtttcacgac gctagtaaac cggaatcgcc
    63061 atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt aatttgatgc
    63121 ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa
    63181 atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt
    63241 gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg
    63301 tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca
    63361 gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg
    63421 cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt
    63481 tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg
    63541 attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata
    63601 tcttcgtttt catcagccag accatgaata ccgcttgcgg tcgtggcgtc cacttcgccc
    63661 accataattg acgaacgctc actggtgccc cccgggcttg ccaggaaact taacaccggt
    63721 ttggtccgtt tgactatcag tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc
    63781 acactttcac cctcttcaat catcccggca accatctcca gtagccaggg ggtttcgctg
    63841 gtgtcgtacg cggcaatccg aatctgctca atcagcacaa cttcatcacg cactgggtca
    63901 aagggtagca agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc
    63961 atttgcccgt tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga
    64021 taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg
    64081 tctggcttaa gcattgttaa tgtcctagca ctaatagtga attaaatgtg aatttcagcg
    64141 acgtttgact gccgtttgag cagtcatatg ttaaattgag gcacattaac gccctatggc
    64201 acgtaacgcc aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt
    64261 tgatcgcgct aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc
    64321 ttatcggcct gagcctttct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag
    64381 tttatcagca agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg
    64441 ctgcctttga aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg
    64501 cagattacac ctatagcaac ggctaccgcg acgcgaacgg catcaactcg aacgcgacca
    64561 gtgcgtccct gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc
    64621 tgcaggaaaa agcagcaggg attcaggacg tcacatatca gaccgatcag caaaccttga
    64681 tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt ctttcctata
    64741 cacaggcaca aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg
    64801 tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg
    64861 cgaacgaagt gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca
    64921 ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac
    64981 cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg
    65041 cacgcttgag ccaggacctg gagcgcgagc aaattcgcca ggcgcaggat ggtcacttac
    65101 cgactctgga tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa
    65161 cccgtggtgc cgctggtacc cagtatgacg atagcaatat gggccagaac aaagttggcc
    65221 tgagcttctc gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggcac
    65281 agtacaactt tgttggtgcc agtgagcaac tggaaagcgc acatcgtagc gtcgtgcaaa
    65341 ccgtacgttc ctccttcaac aacattaatg cttctatcag tagtattaac gcctacaaac
    65401 aagccgtagt ttccgcgcaa agctcattag acgcgatgga agcgggctac tcggtcggta
    65461 cgcgtaccat tgttgatgtg ttggatgcga ccaccacgct gtacaacgcc aagcaagagc
    65521 tggcgaatgc gcgttataac tacctgatta atcagctgaa tattaagtca gccctgggta
    65581 cgttgaacga gcaggatttg ctggcactga acaatgcgct gagcaaaccg gtttccacta
    65641 atccggaaaa cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc
    65701 ctgatagccc ggcacccgtc gttcagcaaa catccgcacg cactaccacc agtaacggtc
    65761 ataacccttt ccgtaactga tgacgacgac ggggcttcgg ccccgtctga acgtaaggca
    65821 acgtaaagat acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc
    65881 catcctctat tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa
    65941 aatccatacg ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg
    66001 ctctcgcggt tgccactgtt tttatgctgg ctggctgtga aaagagtgat gaaacagtgt
    66061 ctctctatca aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta
    66121 ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc
    66181 gtgaagactg tgttgctgaa tttggtgaag gtcagtgtca gcaagcacca gcccaggctg
    66241 gcatggcacc agaaaaccag gcgcaggctc agcaatccag cgggagtttc tggatgccgc
    66301 tgatggccgg ttacatgatg gggcgtctga tgggcggcgg cgcgggattt gcacagcagc
    66361 cgctgttctc ctcgaaaaac ccggccagtc cggcttacgg taaatatacc gacgcgacgg
    66421 gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg
    66481 caccaaaacc ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa tctgttgcca
    66541 aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat
    66601 accgatggaa agagtcagta ttaccgagcg cccggactgg cgtgagaaag cccacgaata
    66661 cggtttcaat tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa
    66721 gttgaccctc gcccaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg
    66781 cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg atgaccaaat tccgcatacc
    66841 aaaacacacc tggagttttg tgcgtcaatc atggctgact caccagccat cgctttattc
    66901 gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga
    66961 tacgccaacg tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct
    67021 taacgccggt aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaaactgat
    67081 cgatcgcttc gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg
    67141 tcgcgacacg gtggaagatc gcggaaccat tcagtatttg caggactgcg caaccgaagc
    67201 tgaaatcgcc accgagttcc tctacatcga cgagataggt ttaggcgaaa aaggccagtt
    67261 cacggattta caggatcagg tgatttctaa cctgttcaag ctttatccgt gggaatttat
    67321 gctgcgcgaa atgttctcca ccaagctgga agatgctggc gtacgctggc tggaaccggc
    67381 gtggaagagc attatctcca acaaggcact tctaccgcta ctgtgggaga tgttcccgaa
    67441 tcacccgaac ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata
    67501 tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa
    67561 aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca
    67621 cccgttaccg aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca
    67681 acctgccgga attggcattc gtgaagaccg tgcgttgatc acccaggata tgtctcggtt
    67741 ttatccacat atttttgttg aataagccac gataccggat ggcactcgcc atccggtaat
    67801 tgttagccta tctgcaccga cagcatactt aaactgccca tttctatacc atcgaccgga
    67861 atggtaattg gctcctgccc atcccacaca cctaacacat acaacaacgg cagatagtgc
    67921 tctggcgttg ggttcaataa cgcgccacct tcatggtcga gataattcac cagaggatgc
    67981 tgctctaccg gcccttgcca cgtcagattc gctttcacat actcattaaa cgacgtcgcc
    68041 cacggatacg gtgaactatc accgtgccac ttcactgtgc gcaggttatg caccacgtta
    68101 ccgctggcga ccaacattat tccttcatca cgcagcgccg cgagtttgcg ccccatttcg
    68161 aaatgccagg cggcaggttt gctactgtcg atactcaact gcaccatagg gatatcggcg
    68221 tcaggataca tcttaatcag cacgccccac gagccgtggt caaagcccca ggcttcttta
    68281 tccagcgtca ccgggatcgg cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa
    68341 cccggagcag gataatgcgt atcgtacagc gcctgcggga agccaccaaa gtcatgaatc
    68401 gtgggcggcg tctccatcgc ggtcactcct gttccacggg taaaccagtg agccgaaacc
    68461 accacaatcg cttgcgggcg tggcaatgtc atccccaact tctgccagct gcgggtatac
    68521 aaattatctt ccagcacgtt catcggacta ccgtgaccta aaaacaatgc tggcatacgt
    68581 gttgaagaca tgatgatatc cttaactaaa ggtgtcattt tgatatcctc acaatacgct
    68641 tgttcggcgg agtaagaacc cggataacaa tgatgatgat catcagttat tttgacgatc
    68701 tgcctgaagg tgaagattta taaggagttg tcgatgtcag tacctctcat tctgaccata
    68761 ctggcggggg cagccacgtt tattggcgcg tttctcggcg ttctcggaca aaaaccctcg
    68821 aaccgcttac tggcgttttc gctaggtttt gcggcgggga tcatgttgct catctcatta
    68881 atggaaatgc ttcctgccgc actagcagct gaaggaatgt cgccggtgtt gggttatgga
    68941 atgtttatct tcggtctgct tggctacttt ggcctggacc gcatgttgcc gcatgcgcat
    69001 ccgcaggatt taatgcaaaa atcggtgcag ctgttgccaa aatcgatcaa gcgcacagcc
    69061 attctgctca ctctcggcat cagtctgcat aacttcccgg aagggattgc cacctttgtc
    69121 acggcgagca gcaacctgga gctgggattt ggcatcgcac tggccgtcgc gttgcacaat
    69181 atccctgaag gtctggcagt ggcaggcccg gtttatgcgg caacggggtc taaacgcacc
    69241 gcgattctgt gggcggggat ttctggacta gcagaaattc ttggtggtgt gctggcgtgg
    69301 ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg caatcatggc ggcggttgca
    69361 ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct
    69421 aataataacc ccagctatgg cgtactatgt ggaatgtcag tgatgggatt cagtttagtg
    69481 ctgctacaaa cggcgggaat tggttaaaaa caacatcaga agttgtccag cacatggaca
    69541 acttctgcca gcttcacgaa agccttattc taccttttcg gctgtctctt ctctcgtact
    69601 gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca
    69661 ataacgtttc aaatgcagga aataacccac cacaaccaga gcaatcaata caatccagta
    69721 ccaggcgata aacatccttt tacccttaat atttaacaat actaaacatt tagcgcataa
    69781 atttcacata tcctttttcg gatatattca tatggtcgca taataacgga acaattatat
    69841 gattttgatc ataatgaaaa agaatgcctg atattaatga aattttctca tagaaagaga
    69901 agcggaatta acatcttgca tagcagggca aaataaaaac cgaagccagc tacaacttcg
    69961 gtttcttgat taaggcagta attaagcagc ggttttcagc tggctttacg ctcatgtgcc
    70021 tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt attggcaaac
    70081 tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc acacagcaca
    70141 ccagccggtt taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca
    70201 cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga
    70261 tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg atcagcggca
    70321 gaaacaccgg tagtcacacc ttcagccgct tcaatggtca cggtaaaacc ggtgccatag
    70381 gcgctggtgt tattttctac catcattggc agatcaagtt gtttacggcg atcttcagtg
    70441 atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc
    70501 atggtttctg ccgggaagat catatcgcct tcgttttcac ggtcttcatc atcaagcacc
    70561 attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc gaaaggcgta
    70621 ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa ttatggttac
    70681 cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga gcgggtccat
    70741 gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc ggaattacac
    70801 cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat
    70861 ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact ataacgctaa
    70921 tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta cccttatcgc
    70981 actacaatgg tactcaacac ctatcagtac agggaaacta caatgattga cccgaaaaaa
    71041 attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag ggagttcggg
    71101 gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg cctcgacctt
    71161 gtaagccgtg aagagttcga cgtccaaacg caagtcctgt tacggactcg tgagaaactg
    71221 gcgctgcttg aacaaagaat cagcgaactg gaaaatcgca gtactgagat caaaaagcag
    71281 ccagatccag agactctccc cccaacgctg taaacatttg tctttaatgc actgtaaatt
    71341 atgaattaat cctctaattt acagtgtttt ctattacatt atcgcttaat cccattcaga
    71401 gtatcatcac aaaaatgaat gattgcaatt gaaaatataa ttgttatgta ttctttaagt
    71461 tttac
//
LOCUS       DAJTPG010000005        70669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTPG010000005 DAJTPG010000000
VERSION     DAJTPG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..796
                     /locus_tag="OO230_000380"
     CDS             <1..796
                     /locus_tag="OO230_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5690267.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            807..1898
                     /locus_tag="OO230_000381"
     CDS             807..1898
                     /locus_tag="OO230_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5690268.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1941..2801)
                     /gene="rsmI"
                     /locus_tag="OO230_000382"
     CDS             complement(1941..2801)
                     /gene="rsmI"
                     /locus_tag="OO230_000382"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5690269.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2866..4902
                     /gene="lpoA"
                     /locus_tag="OO230_000383"
     CDS             2866..4902
                     /gene="lpoA"
                     /locus_tag="OO230_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5690270.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4860..5255
                     /locus_tag="OO230_000384"
     CDS             4860..5255
                     /locus_tag="OO230_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5690271.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5275..5865
                     /gene="diaA"
                     /locus_tag="OO230_000385"
     CDS             5275..5865
                     /gene="diaA"
                     /locus_tag="OO230_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5690272.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5875..6450
                     /gene="yraP"
                     /locus_tag="OO230_000386"
     CDS             5875..6450
                     /gene="yraP"
                     /locus_tag="OO230_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5690273.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6564..7604)
                     /locus_tag="OO230_000387"
     CDS             complement(6564..7604)
                     /locus_tag="OO230_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5690274.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVSGLALLF"
     gene            complement(7677..8312)
                     /locus_tag="OO230_000388"
     CDS             complement(7677..8312)
                     /locus_tag="OO230_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5690275.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8440..8958
                     /gene="yhbO"
                     /locus_tag="OO230_000389"
     CDS             8440..8958
                     /gene="yhbO"
                     /locus_tag="OO230_000389"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5690276.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8938..9381)
                     /locus_tag="OO230_000390"
     CDS             complement(8938..9381)
                     /locus_tag="OO230_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5690277.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9432..9734
                     /gene="yhbQ"
                     /locus_tag="OO230_000391"
     CDS             9432..9734
                     /gene="yhbQ"
                     /locus_tag="OO230_000391"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5690278.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9721..10224)
                     /locus_tag="OO230_000392"
     CDS             complement(9721..10224)
                     /locus_tag="OO230_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5690279.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10218..10742)
                     /locus_tag="OO230_000393"
     CDS             complement(10218..10742)
                     /locus_tag="OO230_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5690280.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10951..11946
                     /locus_tag="OO230_000394"
     CDS             10951..11946
                     /locus_tag="OO230_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5690281.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11955..12833
                     /locus_tag="OO230_000395"
     CDS             11955..12833
                     /locus_tag="OO230_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5690282.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12914..13921
                     /locus_tag="OO230_000396"
     CDS             12914..13921
                     /locus_tag="OO230_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5690283.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14039..15283)
                     /gene="mtr"
                     /locus_tag="OO230_000397"
     CDS             complement(14039..15283)
                     /gene="mtr"
                     /locus_tag="OO230_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5690284.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15437..17326)
                     /gene="deaD"
                     /locus_tag="OO230_000398"
     CDS             complement(15437..17326)
                     /gene="deaD"
                     /locus_tag="OO230_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5690285.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17319..17399)
                     /gene="yrbN"
                     /locus_tag="OO230_000399"
     CDS             complement(17319..17399)
                     /gene="yrbN"
                     /locus_tag="OO230_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5690286.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17506..18390)
                     /gene="nlpI"
                     /locus_tag="OO230_000400"
     CDS             complement(17506..18390)
                     /gene="nlpI"
                     /locus_tag="OO230_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5690287.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18499..20634)
                     /gene="pnp"
                     /locus_tag="OO230_000401"
     CDS             complement(18499..20634)
                     /gene="pnp"
                     /locus_tag="OO230_000401"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5690288.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20881..21150)
                     /gene="rpsO"
                     /locus_tag="OO230_000402"
     CDS             complement(20881..21150)
                     /gene="rpsO"
                     /locus_tag="OO230_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5690289.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21299..22243)
                     /gene="truB"
                     /locus_tag="OO230_000403"
     CDS             complement(21299..22243)
                     /gene="truB"
                     /locus_tag="OO230_000403"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5690290.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22243..22644)
                     /gene="rbfA"
                     /locus_tag="OO230_000404"
     CDS             complement(22243..22644)
                     /gene="rbfA"
                     /locus_tag="OO230_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5690291.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22808..25456)
                     /gene="infB"
                     /locus_tag="OO230_000405"
     CDS             complement(22808..25456)
                     /gene="infB"
                     /locus_tag="OO230_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5690292.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25481..26968)
                     /gene="nusA"
                     /locus_tag="OO230_000406"
     CDS             complement(25481..26968)
                     /gene="nusA"
                     /locus_tag="OO230_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5690293.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26996..27448)
                     /gene="rimP"
                     /locus_tag="OO230_000407"
     CDS             complement(26996..27448)
                     /gene="rimP"
                     /locus_tag="OO230_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5690294.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27655..27731)
                     /locus_tag="OO230_000408"
     tRNA            complement(27655..27731)
                     /locus_tag="OO230_000408"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27695..27697),aa:Met,seq:cat)
     gene            28079..29422
                     /gene="argG"
                     /locus_tag="OO230_000409"
     CDS             28079..29422
                     /gene="argG"
                     /locus_tag="OO230_000409"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5690295.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29430..31037)
                     /locus_tag="OO230_000410"
                     /pseudo
     CDS             complement(29430..31037)
                     /locus_tag="OO230_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31515..31601)
                     /locus_tag="OO230_000411"
     tRNA            complement(31515..31601)
                     /locus_tag="OO230_000411"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31565..31567),aa:Leu,seq:gag)
     gene            complement(31616..31948)
                     /gene="secG"
                     /locus_tag="OO230_000412"
     CDS             complement(31616..31948)
                     /gene="secG"
                     /locus_tag="OO230_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5690296.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32176..33513)
                     /gene="glmM"
                     /locus_tag="OO230_000413"
     CDS             complement(32176..33513)
                     /gene="glmM"
                     /locus_tag="OO230_000413"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5690297.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33506..34354)
                     /gene="folP"
                     /locus_tag="OO230_000414"
     CDS             complement(33506..34354)
                     /gene="folP"
                     /locus_tag="OO230_000414"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5690298.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34444..36378)
                     /gene="ftsH"
                     /locus_tag="OO230_000415"
     CDS             complement(34444..36378)
                     /gene="ftsH"
                     /locus_tag="OO230_000415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5690299.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36478..37107)
                     /gene="rlmE"
                     /locus_tag="OO230_000416"
     CDS             complement(36478..37107)
                     /gene="rlmE"
                     /locus_tag="OO230_000416"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5690300.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37233..37526
                     /gene="yhbY"
                     /locus_tag="OO230_000417"
     CDS             37233..37526
                     /gene="yhbY"
                     /locus_tag="OO230_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5690301.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37682..38158)
                     /gene="greA"
                     /locus_tag="OO230_000418"
     CDS             complement(37682..38158)
                     /gene="greA"
                     /locus_tag="OO230_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5690302.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38406..39839
                     /gene="dacB"
                     /locus_tag="OO230_000419"
     CDS             38406..39839
                     /gene="dacB"
                     /locus_tag="OO230_000419"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5690303.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40025..41197)
                     /gene="cgtA"
                     /locus_tag="OO230_000420"
     CDS             complement(40025..41197)
                     /gene="cgtA"
                     /locus_tag="OO230_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5690304.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41213..42178)
                     /locus_tag="OO230_000421"
     CDS             complement(41213..42178)
                     /locus_tag="OO230_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5690305.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42305..42562)
                     /gene="rpmA"
                     /locus_tag="OO230_000422"
     CDS             complement(42305..42562)
                     /gene="rpmA"
                     /locus_tag="OO230_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5690306.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42583..42894)
                     /gene="rplU"
                     /locus_tag="OO230_000423"
     CDS             complement(42583..42894)
                     /gene="rplU"
                     /locus_tag="OO230_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5690307.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43152..44123
                     /gene="ispB"
                     /locus_tag="OO230_000424"
     CDS             43152..44123
                     /gene="ispB"
                     /locus_tag="OO230_000424"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5690308.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44350..44628
                     /gene="sfsB"
                     /locus_tag="OO230_000425"
     CDS             44350..44628
                     /gene="sfsB"
                     /locus_tag="OO230_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5690309.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44676..45935)
                     /gene="murA"
                     /locus_tag="OO230_000426"
     CDS             complement(44676..45935)
                     /gene="murA"
                     /locus_tag="OO230_000426"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5690310.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45990..46244)
                     /gene="ibaG"
                     /locus_tag="OO230_000427"
     CDS             complement(45990..46244)
                     /gene="ibaG"
                     /locus_tag="OO230_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5690311.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46404..46697)
                     /gene="mlaB"
                     /locus_tag="OO230_000428"
     CDS             complement(46404..46697)
                     /gene="mlaB"
                     /locus_tag="OO230_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5690312.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46697..47332)
                     /gene="mlaC"
                     /locus_tag="OO230_000429"
     CDS             complement(46697..47332)
                     /gene="mlaC"
                     /locus_tag="OO230_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5690313.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47351..47902)
                     /gene="mlaD"
                     /locus_tag="OO230_000430"
     CDS             complement(47351..47902)
                     /gene="mlaD"
                     /locus_tag="OO230_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5690314.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47907..48689)
                     /gene="mlaE"
                     /locus_tag="OO230_000431"
     CDS             complement(47907..48689)
                     /gene="mlaE"
                     /locus_tag="OO230_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5690315.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48697..49506)
                     /gene="mlaF"
                     /locus_tag="OO230_000432"
     CDS             complement(48697..49506)
                     /gene="mlaF"
                     /locus_tag="OO230_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5690316.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49716..50681
                     /locus_tag="OO230_000433"
     CDS             49716..50681
                     /locus_tag="OO230_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5690317.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50695..51681
                     /gene="kdsD"
                     /locus_tag="OO230_000434"
     CDS             50695..51681
                     /gene="kdsD"
                     /locus_tag="OO230_000434"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5690318.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51702..52268
                     /gene="kdsC"
                     /locus_tag="OO230_000435"
     CDS             51702..52268
                     /gene="kdsC"
                     /locus_tag="OO230_000435"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5690319.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52265..52840
                     /gene="lptC"
                     /locus_tag="OO230_000436"
     CDS             52265..52840
                     /gene="lptC"
                     /locus_tag="OO230_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5690320.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52809..53366
                     /gene="lptA"
                     /locus_tag="OO230_000437"
     CDS             52809..53366
                     /gene="lptA"
                     /locus_tag="OO230_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5690321.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53373..54098
                     /gene="lptB"
                     /locus_tag="OO230_000438"
     CDS             53373..54098
                     /gene="lptB"
                     /locus_tag="OO230_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5690322.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54146..55579
                     /gene="rpoN"
                     /locus_tag="OO230_000439"
     CDS             54146..55579
                     /gene="rpoN"
                     /locus_tag="OO230_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5690323.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55602..55889
                     /gene="hpf"
                     /locus_tag="OO230_000440"
     CDS             55602..55889
                     /gene="hpf"
                     /locus_tag="OO230_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5690324.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56007..56498
                     /gene="ptsN"
                     /locus_tag="OO230_000441"
     CDS             56007..56498
                     /gene="ptsN"
                     /locus_tag="OO230_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5690325.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56544..57398
                     /gene="rapZ"
                     /locus_tag="OO230_000442"
     CDS             56544..57398
                     /gene="rapZ"
                     /locus_tag="OO230_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5690326.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57395..57667
                     /gene="npr"
                     /locus_tag="OO230_000443"
     CDS             57395..57667
                     /gene="npr"
                     /locus_tag="OO230_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5690327.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57880..58512
                     /gene="yrbL"
                     /locus_tag="OO230_000444"
     CDS             57880..58512
                     /gene="yrbL"
                     /locus_tag="OO230_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5690328.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58509..59236)
                     /gene="mtgA"
                     /locus_tag="OO230_000445"
                     /pseudo
     CDS             complement(58509..59236)
                     /gene="mtgA"
                     /locus_tag="OO230_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59233..59886)
                     /gene="elbB"
                     /locus_tag="OO230_000446"
     CDS             complement(59233..59886)
                     /gene="elbB"
                     /locus_tag="OO230_000446"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5690329.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60116..62452)
                     /gene="arcB"
                     /locus_tag="OO230_000447"
     CDS             complement(60116..62452)
                     /gene="arcB"
                     /locus_tag="OO230_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5690330.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62548..63477)
                     /locus_tag="OO230_000448"
     CDS             complement(62548..63477)
                     /locus_tag="OO230_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5690331.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64152..68612
                     /gene="gltB"
                     /locus_tag="OO230_000449"
     CDS             64152..68612
                     /gene="gltB"
                     /locus_tag="OO230_000449"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5690332.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68625..70043
                     /gene="gltD"
                     /locus_tag="OO230_000450"
     CDS             68625..70043
                     /gene="gltD"
                     /locus_tag="OO230_000450"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5690333.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70227..>70669
                     /locus_tag="OO230_000451"
     CDS             70227..>70669
                     /locus_tag="OO230_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690334.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLY"
BASE COUNT        17301 a        19167 c        17553 g        16648 t
ORIGIN      
        1 ctggttaccg cgtagcctgg tttcctatca gatgaccagc cagaaagatc gcccaaccca
       61 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
      121 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
      181 ttatggaacc ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg
      241 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
      301 cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg aggatacaaa actatccggg
      361 gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt atcaggaaaa
      421 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgaac aaacaacaca
      481 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
      541 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
      601 cagcaacgat gatacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
      661 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca
      721 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
      781 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
      841 tgatatccac atcctgcgct tatgcctcct caggagggtg tggagccgac agcactagcg
      901 gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca gataacgtga
      961 caggacggga gtttacctct gcaacgctaa gtagcactaa ctggcaatac gcctgttcct
     1021 gctctgcggg taaggcagtt aaacttgtct atatggtcag ccccgtactt accaccactg
     1081 gacatcagac aggatattac aaactcaatg acagcctgga tattaaaacc acattacagg
     1141 caaacgacat tccaggactc acaaccgacc aggttgtctc tgttaacacc cgattcacac
     1201 agataaaaag cagcaccgta tattctgctg caacccaaac gggtgtttgc cagggtgaca
     1261 cgtctcgtta tggacccgtt aatattggtg caaataccac ctttaccctg tatgtcacca
     1321 agccatttct cggctcgatg accattccga aaacggatat tgccgtcatt aaaggcgcgt
     1381 gggtcgatgg aatgggaagc ccgtctacag gtgacttcca tgatttagtc aagttatcga
     1441 ttcagggaaa tctcaccgcc ccacagtcgt gcaaaattaa tcagggcgat gttattaaag
     1501 ttaattttgg attcatcaat ggtcagaagt ttaccacccg caatgccatg ccagacggtt
     1561 ttactccagt agactttgat atcacttatg actgtggtga tacttcaaag attaaaaact
     1621 cgttgcaaat gcgcatcgac ggtacaactg gggtagtaga ccagtacaac ctggtcgcca
     1681 ggcgaagaag ttcagacaat gtgcccgatg tcggtattcg tattgaaaat ctcggcggcg
     1741 gagttgcaaa tattcctttt cagaacggta tccttcccgt tgatccttcc gggcatggca
     1801 ccgtcaatat gcgcgcctgg ccagttaatc tggtcggtgg tgagctggaa acaggaaaat
     1861 ttcagggcac agccaccatt accgtcatgg tgcggtaaaa gaagacgttg cgttttgatt
     1921 aacgcaacgc ttcggcactg ttacccctgc tgctccagcg catacttata cagcgcattt
     1981 ttcttcacgc catgaatttc tgcggccagc gccgccgctt ttttcagcgg cagttctgcc
     2041 tgtagcagcg ccagcgtgcg cagggcatcg gcgggtaagt cttcttcctg tgctttatga
     2101 ccttcgacaa tcagcaccat ttcgcctttg cgacggtttt catcttcctt tacccacgcc
     2161 agcagctcgc caacgggcgc gccgtgaatg gtttcccagg ttttggtcag ctcacgcgcc
     2221 agaaccacgt agcgggattc gcctaatacc gcaacgatat cttccaggct atctaacaga
     2281 cggtgggtag attcataaaa aatcagcgtg cgcggctccg cttcaatggc ttttagcgca
     2341 tcacggcggc cttttgattt ggcaggtaaa aagccttcgt aacagaaacg gtcagatggt
     2401 aaacccgcgg cgcttaacgc agtgatagca gcacacggtc cgggtagcgg caccacacga
     2461 atccccgctt cacggcaggt acgcaccaga tggtagccag gatcgttaat tagcggcgtt
     2521 ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca gctttgccag cagcgtttcg
     2581 gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc gggcattaat cccaaaatgt
     2641 tgcagcaata aaccggtgtg acgagtatcc tcggcggcaa tcagatcaac ggcctgtaat
     2701 acctctaacg tacgctgggt gatatccgcc agattgccga ttggcgtcgg tacaatgtaa
     2761 agctggccct gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg
     2821 atttaatatt gagcattgcg taaaaaaaat atcactggat acattatggt accctcaaca
     2881 ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc
     2941 ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac ggcgcaggct
     3001 gattctgcct tttatcttca gcagatgcag caaagctctg atgataccag gatcaactgg
     3061 caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc ggttgagttg
     3121 tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg
     3181 gcagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc
     3241 acacctgccg atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa aatcgatgcc
     3301 agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctt
     3361 ggcgcgaaag aaaaacagca gaatattgat gccacctggc aggcgctctc ctccatgact
     3421 caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca aggctggctg
     3481 gatctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa agccgggatc
     3541 gccgactggc agaaacgtta tccgaacaat ccgggcgcga aaatgctgcc aacgcagttg
     3601 gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct gttgccgctg
     3661 aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc cgcgaaaaat
     3721 atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga cgtagcagaa
     3781 caacctcagc cgcaaactgc ggatagcgtt gccagcccgg cacaagcctc ggttagcgat
     3841 ctgaccggtg atcagcctgc ggcccagccg gtgcctgtaa gcgccccggc gacaagcacc
     3901 gcagcggtaa gcgcacccgc aaatccatcc gcagagctga aaatctacga tacctcatca
     3961 caaccactta gccagatctt aagccaggtt cagcaggatg gcgcgagtat tgtggttggt
     4021 ccgttgctga aaaataacgt tgaagagttg ctgaagagca acaccccgct gaacgtactg
     4081 gcactgaacc agccggagaa tatcgaaaac cgcgtcaata tttgttactt cgcgctttca
     4141 ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg
     4201 ctggtgctga tcccacgcag tgcattgggc gatcgcgtag ccaatgcgtt tgcgcaagag
     4261 tggcagaaac tgggcggcgg caccgttctg caacaaaaat ttggttccac cagcgaatta
     4321 cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg gtagcccgat tactcccaga
     4381 gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac gccaaccgat
     4441 gaccagttca ccaataatgg cggtcgtgtc gatgcggtct acattgtggc aacgccgggt
     4501 gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag cggtgcaacg
     4561 ctgtacgcca gttcccgcag cgcacaaggc accgcaggcc cggatttccg actggagatg
     4621 gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctgcc gttaatgcag
     4681 caggcgctca gcgcggtgaa caacgattat tcgctggctc gcatgtatgc gatgggcgtt
     4741 gatgcctggt cgttggcaaa tcatttctca caaatgcgcc aggttcaggg ttttgaaatc
     4801 aacggtaata ccggaagtct gacggctaac ccggattgcg tgattaacag gaagttatca
     4861 tggctacagt accaacaagg tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg
     4921 atgcgtggga agcacaagcg cgtcgctggc tggaaggcaa aggactgcgg tttatcgccg
     4981 ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc ctgaccaccg
     5041 tttttgtcga ggtacgctat cgccgctctg cgctttatgg cggcgcggca gccagtgtga
     5101 cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg cgtcataatg
     5161 ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg aatgaggttg
     5221 agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat tagcgtgcaa
     5281 gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc ggcagaggcg
     5341 cttccggatg ccatctcccg tgcagccatg acgctggttc agtctctgct caatggcaac
     5401 aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt tgctgccagc
     5461 atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact aaatactgat
     5521 aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta tgcaaaacag
     5581 gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg tggcaacagc
     5641 cgcgatattg ttaaagcagt tgaagccgcc gttacgcgtg atatgaccat tgtggcattg
     5701 accggctatg acggcggcga acttgcaggt ttgttagggc cacaggatgt ggagatccgc
     5761 attccttcgc atcgtagtgc tcgcattcag gaaatgcata tgctgacggt aaattgcctg
     5821 tgcgatctga tcgataacac gcttttccct caccaggatg attaaggaga atacatgaag
     5881 gcattatcgc caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg tgttgccgct
     5941 gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa ctgacccacg cagtgtcggc
     6001 acccaggtgg acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc gaaagacgaa
     6061 cagattaaga aagaaacccg tattaatgtc acggcctatc agggcaaagt gctgctggtt
     6121 gggcagtcac caaatgctga actttcggct cgcgccaaac agattgctat gggcgtagac
     6181 ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct gggcgaagca
     6241 tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag cgacctggtg
     6301 aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat ggggctggtg
     6361 actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtgagcgg cgtaaagcgg
     6421 gttaccacag cgtttacgtt tattaaatag cagccccttg taatgcctga tgcgacgctt
     6481 gccgcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc gtttacgccg
     6541 catccgacat gttttccctc aaatcagaac aacagcgcca gcccgctgac aatcacacca
     6601 gacactgcca ccatcgcccc tgtcagccat aaggctttcg ccgggaacgc tttgcgcagc
     6661 ataatcagtg acggcaaact cactgccggg agcgtcatca acaatgccag cgccggagcg
     6721 gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc cgtgggaatg
     6781 acaaacaagc atcctgctac cgccatcgcc accacccaca tcaggctgtt atcgacagca
     6841 ccatcggcat gggggaataa ccagacgcgt gcggcaccca acaccagtac tgcaaggatg
     6901 taaaccggga tcgtactcca gaaaagtgtc catagcgccc tgccccagcg gctaaaaaaa
     6961 ccgctctgtg cttccggtat gtcaatttcg accggtgcct gcgtttgcgg tgtttcacgc
     7021 acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagcccggc caccagacga
     7081 atcgccgcaa aaccccagcc gaggacaaac cccataaaca ccagcgtcgc cgggtttaac
     7141 accggattgc ccatccagaa tgccagcgca ccgcccatcg atacctgttg gcgacgcatt
     7201 cccgccgcga ccggagccgc acagcaggta cacatcatgc ccggcagcga aaacagcgtt
     7261 cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg
     7321 atcagcacct gaatcaacga accgaggatc acccccagca ccgccgcttt ccatactgcg
     7381 aggaaataga tcatcgcgta atccaacgcc gcttgccacg gattagcatc cgcctgcgca
     7441 aggatagatt taccgatact gtgggtttcg gctgcagtaa acgctttgcc gtagtaaggt
     7501 tgccatttca cataccagag accggcaatg acaacgagaa agaaaagcgc gggtttccac
     7561 cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt ccccaggaat
     7621 aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg gaataattat
     7681 tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca cgccttcatg ctcaggccgc
     7741 atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt ccagttacct
     7801 ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt gctacgatcg
     7861 cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc aattaatgct
     7921 tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt ggcacccata
     7981 gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc cggttaatgc ggtatccact
     8041 accagcgtgt aatcggcatg aataaacgcc tctttgctcc ccgcttctcg ccgcgtggtg
     8101 ccgagacaac aaaacacaat gtcgatgggt tcggtgacct gcgccaacgc gtcactcagt
     8161 tgcggatcat ggggattaaa caccccgggc atatcgccca acggacgtcg cgtcggcgca
     8221 gcaatggcgt taactttcgg ttcattaatc aacatccgca gcaggtgacc gcccaccagc
     8281 cccgttgcgc ctgtaatcag tacctgactc atcttcgttc ctttacagaa ttgtctgcct
     8341 tgcgctcccc ggctcatgca ccagtcttaa tagaccatga ggtaattatc cccgattgtg
     8401 gaaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa aattgccgtt
     8461 ttaatcactg atgaatttga agattcagaa tttacttcac ccgcagacga gttccgtaaa
     8521 gccggacacg aagtgattac tattgaaaaa caagcgggta aaacggtgaa aggcaaaaaa
     8581 ggagaagcca gcgtgaccat cgataaatcc atcgatgaag tgacgccagc ggagtttgat
     8641 gccctgttgc taccgggtgg tcattcaccg gattatctgc gtggcgacaa ccgttttgtc
     8701 acctttaccc gtgattttgt gaatagcggc aaacctgttt tcgccatctg tcacggtccg
     8761 cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgaccgcagt taaaccgatc
     8821 attattgatg ttaaaaatgc gggcgtggaa ttttacgatc aggaagtcgt ggttgataaa
     8881 gatcagctgg ttaccagccg gacaccggac gatctgccag cgtttaaccg cgaggcgtta
     8941 cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt ttaccaaagc ccagcgtgtt
     9001 gtcggtgaat ttgatttcat cgagccggat ttcccacacc ggggccgaca gcattctggc
     9061 aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg ctttcctcac cttccagcct
     9121 gcggatctca cctttaaact gcacaccgcg aattaacgct accgttttcg gctgaccgtt
     9181 taccgttccg gcaaccgcag cctgcggccc actcatctgc gcgtggcgcg ttttttcttc
     9241 cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga taaaaggcat tagcgcacca
     9301 gagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca gccaacggct
     9361 gatggcgatg agtgtttcca ttgctgttct cttttatact gtgggcctga ccttaacatg
     9421 cgatgcagac gatgacaccc tggtttcttt acttgatccg taccgccgac aataagcttt
     9481 ataccgggat caccacggat gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg
     9541 cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt ttctgcgcca gtgggcgacc
     9601 gttcgctggc actacgggcg gaatatcgcg ttaagcaact gaccaaacgg caaaaagagc
     9661 gtctggtagc ggaaggcgca gggtttgcag aactgttgag cagcctgcaa accccggaga
     9721 ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg gttacgccat tcagcgcgtc
     9781 atccgctaaa cgatgtacca ggaaggcgct ttcagtgccc ggccagcggc aacgcagatc
     9841 gtgatgcgcc gccagttcaa agccgaaacg actgtacagc gccggatcgc ccagcgtcac
     9901 cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca gttggcgtgc
     9961 cagcccctgt ccacggtatt tttcatcaac cgccaacggt gccatgccga cccattgcag
    10021 gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct gaccttcgtc
    10081 atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt
    10141 cgcttccgca tcactttcga atgagcgacg cagcagggca tcaatacccg gcgcatcaat
    10201 gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt ttcaggcgcg
    10261 gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg taaggctttc
    10321 ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacacccc cagctccgta
    10381 tcgccttcaa tcaccagccg acgctggaag aagagcgtat ccggatcttg tttacgcgcc
    10441 gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg cgtgttctgg
    10501 ctgaccacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat atcacgcaca
    10561 tgaatactta accagcggcc ttcaagaaac tccagctcgc catcatccag cgcctgacgg
    10621 aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg cgtcagtttt
    10681 accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag tttatccaac
    10741 acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa aaataacata
    10801 gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa ctttaactgc
    10861 cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta acatttttgc
    10921 gttttgatag cgcaaccttc aggaaaaatt atggagctgc tctgccctgc cggaaatctc
    10981 ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg gctaaaagat
    11041 gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt gcaggaagcg
    11101 gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat
    11161 ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg cggcgcagct gggtgccgac
    11221 gcgctgatcc tcgccgacct cgccatgctg gagtacgccg ccgagcgtta cccgcatatt
    11281 gaacgtcatg tgtcagtgca ggcttcggcg accaatgaag aggcgattaa cttttatcat
    11341 cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt tgtcgattca tcaggtgaaa
    11401 cagctggcac gggttactcc tgtcccgctg gaagtgtttg ctttcggcag cctgtgtatt
    11461 atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc caacactgtg
    11521 ggtgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa cgccgcaggg gctggaatcc
    11581 cgcctgaacg aagtgctgat cgaccgttat caggacggcg aaaacgcagg ttatccgacg
    11641 ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga agaaccaacc
    11701 agtctcaata ccctggaact gctgccggag ttaatggctg cgaatattgc ttcggtgaaa
    11761 attgaaggcc gccagcgtag cccggcgtat gtcagccagg cggcgaaagt ctggcgtcag
    11821 gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac cgcaaagcgc gtggatggag
    11881 acgctcgggt cgatgtccga aggcacgcaa accacccttg gcgcgtatca ccgtaaatgg
    11941 cagtgagaag agcaatgaaa tattccttag ggccagtgct gtggtactgg ccaaaagaga
    12001 cgctggaaga tttttatcag caagccgccg ccagcagcgc cgacgtgatt tatcttggtg
    12061 aagcggtatg cagcaagcgc cgggcaacca aagttggcga ctgtctggag atggcaaaat
    12121 cgctcgccgg aagtggcaag cagattgtgc tctccacgct ggcgctggtg caggcatcct
    12181 ctgaactggg cgaactgaaa cgctatgttg agaacggtga atttctgatt gaagccagcg
    12241 atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc gggcacgcgc
    12301 tgaactgcta caacgcggtg acgctgaaaa tattgctcaa acagggcatg atgcgctggt
    12361 gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag tgcgatgagc
    12421 tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatcta ccgctggcct
    12481 actccgcccg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct
    12541 gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca ggaaaaccaa caagtgtttg
    12601 tactcaatgg cattcagacc atgagcggct acgtttacaa cctcggtaac gagctggcat
    12661 ccatgcaggg attggttgat gtagtccgcc tgtcaccgca gggcactgac actttcgcga
    12721 tgctcgacgc cttccgcgct aatgaaaatg gcgctgtgcc actgccgttg acggcaaaca
    12781 gcgactgtaa cggctactgg cggcggctgg caggactgga gctgcaagcc taagtaaata
    12841 gctcactttg ttaacaactt taactattct ttaatgcagt attaaagatt aatcggtaac
    12901 aaagtgagct gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat
    12961 acccgaaggc tcttcagcgc gggaagcgtt ctcccactct ctcgatctcg cccgtctggc
    13021 tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga ccggcattgc
    13081 cagtgctgca acatcggtat tgattggcta tctggcggcg aataccacca cgctgcatct
    13141 ggggtctggc ggcgtgatgt tgcctaacca cttaccgttg gtcattgccg aacagttcgg
    13201 cacactcaat acgctctatc cggggcgaat cgatttgggg ctgggtcgtg ctccgggtag
    13261 tgaccaacgg acaatgatgg cgctacgtcg tcatatgagc ggcgatattg ataatttccc
    13321 ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg
    13381 cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag
    13441 tgcacaactg gcggcgcagc ttggtctgcc atttgcgttt gcctcacact tcgcgccgga
    13501 tatgttgttc caggcgctgc atctttatcg cagtaacttc aaaccgtcgg ctcggctgga
    13561 aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga
    13621 atttctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccgggca
    13681 actgccgccg ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatgacgt
    13741 gcagcaggcg ctgagtatgt cgttggtagg cgataaagcg aaagtgcgtc atggtttgca
    13801 gtcgatcctg cgcgaaaccg acgctgatga gattatggtt aacgggcaga ttttcgacca
    13861 ccaggcgcgc ctgcattcgt ttgagctggc gatggatgtt aaggaagagt tgttgggata
    13921 gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg taggcctgat
    13981 aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt
    14041 actgatacac cggcagtaaa ttaaagctcg ataaaatatg caccagtgcg ttgccgacgc
    14101 caaacaccag aatcagcgca atcatcggct tgccacccca gacgcggaat ttcgggctgc
    14161 caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc cagatggtag
    14221 ccgctaaacc agcataacca atggcataca ggaatccgtt cgggaacagc agaccaccca
    14281 caacaggcgg ggcaaaggtc agcaatgccg ttttcaagcg gcccacagcc gagtcgtcga
    14341 aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg
    14401 ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc
    14461 cgcttaatgc ttgtaccaac acatcaatat taccgccctt ctcggcgatg ccgataaact
    14521 ccggacgagg gatgttgccc attgtcgcca gcaaccagat ggtatacagc gccagtgcca
    14581 tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca
    14641 tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc agggtcatca
    14701 gcaaatacgg cgcataagat gcattgcttt cggcaacgtt gaacaatgtc gcaggctgca
    14761 catgccccag caggctaccg aaggtgagga agaaggtaat gactttcgcc cccagcacaa
    14821 tcgccgtcat gcggctgacg gctttagtac tcaaccacac cacaaacgct accagcagtg
    14881 caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat
    14941 gcagaatcga accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa
    15001 tggaaatgcc gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa
    15061 aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca
    15121 tacagaacca ggtaaagatc agcgctgcca ttgaccagaa aaaccacgcc ccggacatga
    15181 ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc
    15241 caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct tctctccagt
    15301 gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacatga gtacaaaaga
    15361 cagaaaaaaa gccccgatgg ttaaaatcgg ggctgtatat attattttac agattgtgtt
    15421 cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac
    15481 gacgcggtgc gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacgaccac
    15541 cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgctcgcca ccgaaaccac
    15601 gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt
    15661 tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac
    15721 ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttaccg atgtaacggc
    15781 tgctgatgtc gccttcgtta gcaatcgcgc caacgatatg acgaacttca acaccatcat
    15841 cgcggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg
    15901 gacgatcttc acggtcgcca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat
    15961 cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggaaca atcagagtac
    16021 gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac
    16081 cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt
    16141 ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg
    16201 cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca
    16261 gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg
    16321 tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag ttaaccacca
    16381 ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc ggtcgcaatc aggatgtcca
    16441 gacgaccgtc tttaaggcgt tccagtgtct gctcacgcag cgcctggttc atgtcaccgt
    16501 tcagcgcggc gctattgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt
    16561 ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca
    16621 gtgcttcgtt tttacgcata ccccacacag tccagtagct ctggctgatg tcaggacggg
    16681 tagtcacgct ggactgaata cgcacttcct gcggctcttt cataaagcgg cgggtaatgc
    16741 gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct
    16801 gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat
    16861 ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca
    16921 gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt
    16981 cataacgctg gccgccgtac agggcaacca catttacgcc gcgcatgtgt ttagagaaat
    17041 ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct
    17101 gtggtgcttt cagctcagga tcaagattct gcagcagagg taaagagaat gctgcagttt
    17161 ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac
    17221 actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag
    17281 gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc
    17341 tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt
    17401 gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta
    17461 aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga
    17521 ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg
    17581 ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc
    17641 caaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc
    17701 agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct
    17761 aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc
    17821 gaagtgctgt ttcaatactt ctttagcctg cttctcatcg agcttctgct cggcgagata
    17881 aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc
    17941 ttgcgctaac ttgtcacgac cgccgtaata taatgcaatc ccgcgattca agtgcgcgta
    18001 gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt
    18061 gcctgcctgc gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat
    18121 tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa
    18181 cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag
    18241 gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc
    18301 gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc
    18361 aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga
    18421 acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc
    18481 ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc
    18541 aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac
    18601 ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac
    18661 gcgtttgtca gcgatttgag agatgtgtac cagaccttct ttaccgccgc cgatggcaac
    18721 aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat
    18781 ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc
    18841 tgcaatcttc acagtaccgt catcttcgat ttcgatggtg gtgccagttt cttcggtcag
    18901 ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg ggttgatctt
    18961 gatggtatgg atacgtggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg
    19021 ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat
    19081 gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga taccctcgcg
    19141 ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga
    19201 cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc
    19261 tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga
    19321 agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg ggaatttgtc
    19381 catatccggc atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg
    19441 acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg
    19501 gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc
    19561 agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca gcgcagaacc
    19621 gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg
    19681 accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatcgc
    19741 gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc
    19801 gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg
    19861 gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt
    19921 taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca gttcattgat
    19981 gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca gcatctggtc
    20041 ttcgctcagc agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac
    20101 aaccagatcc agtttgctct ctttcaactc gtcctgagtc gggttcagta cgtactggtc
    20161 attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac cagacagaga
    20221 cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg ggttaacaga
    20281 aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcggccgaat
    20341 cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttgggcggc cttcacgacg
    20401 gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag
    20461 tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa caaataccgc
    20521 ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc
    20581 cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat
    20641 atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg
    20701 catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc
    20761 cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat
    20821 tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa
    20881 ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt
    20941 caggtagtcg agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg
    21001 gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag
    21061 tgctacctga acttcagtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat
    21121 tttagctgtt gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag
    21181 gacgccgatc tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac
    21241 gcgacgttaa gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt
    21301 acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg gccttcatcg tcaatttcgc
    21361 ccataccgat aaatttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg
    21421 caccggatgt acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat
    21481 tcaccaccgg gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact
    21541 ccgcagccgg aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg
    21601 tcacaatccg ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg
    21661 cgccacaacc gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg
    21721 agcagtgaat ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa
    21781 cggtaatcgg gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt
    21841 ttttaccctg atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga
    21901 aagtatccag cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga
    21961 tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc
    22021 gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca
    22081 acatgccagt tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa
    22141 tccgtttcac tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca
    22201 aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc
    22261 gtccgggtta acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat
    22321 gcgcatccct tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag
    22381 gcgcatcgct ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat
    22441 gcccgcttta accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata
    22501 cgccaggtcg cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc
    22561 tttaatttca cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg
    22621 cgggcgacca aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc
    22681 ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag
    22741 cgtcacatca ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct
    22801 aaaaacctta agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc
    22861 cagtgcggac gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt
    22921 cgttaacgtc atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt
    22981 tgtcacgcag aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc
    23041 ctgcgatggc accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa
    23101 tctgctgttt cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca
    23161 ggttatagat gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg
    23221 cagaggcatc agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg
    23281 tggcgtcggt ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt
    23341 cagtagacag tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct
    23401 tcaggacgat gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga
    23461 gtttagattt ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg
    23521 caacttcacg cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta
    23581 cgccggacag gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac
    23641 ccagttcgtt acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc
    23701 ccttgtgcag agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga
    23761 ggaaggattc gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa
    23821 cttccgcctg cagcaggata gcgtccagca gttcatcgat accagtaccc gctttcgcag
    23881 acacatgtac gaactggctt tcaccgcccc actcttccgg caggatgccg tactgggaga
    23941 gttcgttctt aacgcgatcc ggatcggctt ctggtttatc gatcttgttc actgcaacga
    24001 ccaccggaac ctgcgccgct ttcgcgtgct ggattgcttc gatcgtctgc ggcattacgc
    24061 cgtcgtcggc agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgagcacgca
    24121 ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt
    24181 caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gccactttcg
    24241 ttgaacgaat gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca
    24301 caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt
    24361 ccagctcgtt ttcacgacgc aggataactt tatggcccat ctcttcagca accagctgtg
    24421 cggtttcctg atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt
    24481 tgatgacctg agagccttta accgccatct tgttcgccag ttcgccaacg gtgatagttt
    24541 cgccgatcac aacgtcacgg ttaacggcct gagcaggctt ctggaagcct tgctgcagcg
    24601 aagaaccttt acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat
    24661 cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac
    24721 cacggccacg gccgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt
    24781 gagaagtagt gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt
    24841 tgttttcttc cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt
    24901 tacgacgcgc ttcttcttca gctttacgct tgagctctgc agcttcctgc tcacggcggg
    24961 ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt
    25021 ccgcagcttc acgtttcgct tgttcagcag cttcacgctt agcttgttct gcggcctcac
    25081 gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct
    25141 cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc
    25201 ggacttcgat ttgtaccgat ttgctttttc caccggtacc aggaatgtta agggtgctgc
    25261 gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt tttctgattc aggtggtcaa
    25321 tcaaagtctg tttctcttgt gcagacacag agtcgtcagc agacttccgg atacctgcat
    25381 cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa
    25441 tcgttacatc tgtcatgctg ttccttcctg ctacagttta ttacgcttcg tcaccgaacc
    25501 agcaaatatt acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc aacccttcga
    25561 tatcagccag atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa acgccacggg
    25621 cggccagttt gaatgccaaa tcacgatcta ccccttcaag gttcagcaga tcgtcagccg
    25681 gtttgttatc accgaggctt tcttcctggg cctgtgcaat ggtggccagt gcatttttag
    25741 cacgctcgcg cagtgcttca acggtcggct catcaaggcc ttcgatttcc aacagctctt
    25801 tcatcggcac ataggccagt tcttccagcg tcgagaaacc ttcttctacc agaacagtcg
    25861 cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg
    25921 cctgatgctt agcctgcagg tcgtcaacgg tcattacgtt gagttcccaa ccgctcagct
    25981 gcgaagccag acgcacgttc tgaccgttac ggccaatcgc ctgcgccagg ttaccagctt
    26041 caacggcgat atccatggtg tgtttatctt catccaccac gatagaagca acgtctgccg
    26101 gtgccattgc gttaatcaca aactgcgccg ggttatcatc ccacaggacg atatcgatac
    26161 gctcgccgcc cagttcagta gaaaccgcct gaacacgcgc gccacgcata cctacgcaag
    26221 cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc gattttcgca cgagaacccg
    26281 ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc gatttccggc acttcaatac
    26341 ggaacagttc gatcagcatt tccggcttgg aacgagtgac gaacagttgc gcgccacgcg
    26401 cttccgggcg aacggaataa agcacgccac gaacgcggtc gccagggcgg aagttttcac
    26461 gcggcagcat atcttcgcgc aggatcacgg cttcagcgtt gttgcccaga tccagagaga
    26521 tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga
    26581 actgatcaac caccatcgca cgttcggctt cacgcacttt ctgcacgata acctgttttg
    26641 ccgtctgggt ggtgatacgg tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc
    26701 ccaagttcag gctttcatct tcataacgtg cggcttcaag ggtgatttcc ttggtcggct
    26761 gggtgacttc atcaacaact aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat
    26821 cgatctgtac acggacgtcg atctcttgct catatttttt ctttgttgct gtcgccagcg
    26881 cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg gatacggctt
    26941 caactacagc caaaatttct ttgttcatcg cgggcttttc acctcatcca gactattaaa
    27001 agtggggaac caggttcgcc ttctggatat tactcagcgc gaacacttca tctttacctt
    27061 cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat aacgccctgc catttacgac
    27121 ggttttgtac cgccatacgg agaaccagag tcacctcttc tccgacaaaa cgggcgtagt
    27181 gttcagccgt gaacagtggg cgatcgagac ccggtgagga gacttccagg ttataagcaa
    27241 cggtgatggg atcttcaaca tccagcacag cacttacctg gtggctcaca tcagcacaat
    27301 catcaacatt gatgccatct tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc
    27361 gaataaattc gatgcctacc agttcaaaac ccagggcctc aactggcgca gtaatcatct
    27421 ctgttaattt ttgctctaat gtggacaagc ccacccccaa gacataaaaa aagggcctaa
    27481 agcccagtta ttctgtagtc agataacaaa aaaccccgat aaatcggggc tttatataac
    27541 tgaaccctat aaccgcaact gcggtctgga gcactttcca gaaggatttt ttcaaatccc
    27601 actacgaagg ccgaagtctt cacagtatat ttgaaaaagg actctaaggg aaagtggttg
    27661 cgggggccgg atttgaaccg acgaccttcg ggttatgagc ccgacgagct accaggctgc
    27721 tccaccccgc gcctgaaacg tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact
    27781 gctgggattt ggtgtaccga gacgggacgt aaaatctgca ggcattatag tgatccatgc
    27841 cacattttgt caacgtttat tgctaatcac gtgaatgaat atccagttca ctttcatttg
    27901 ttgaatactt ttgccttccc ctgctctccc ttaagcgcat tattttacaa aaaacacact
    27961 aaactcttcc tgtctccgat aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa
    28021 attcagtgag agcggaaatc caggctcatc atcagttaat taagcagggt gttattttat
    28081 gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg
    28141 tctggacacc agtgccgcac tgctgtggat gcgacaaaag ggagcggttc cttatgcata
    28201 tactgcaaac ctgggccagc cagacgaaga ggattatgat gcgatccctc gtcgtgccat
    28261 ggaatacggc gcggagaacg cacgtctgat cgactgccgc aaacaactgg tggccgaagg
    28321 tattgccgct attcagtgtg gcgcatttca taacaccacc ggcggcctga cctatttcaa
    28381 cacgacgccg ctgggccgcg ccgtgactgg taccatgctg gttgctgcga tgaaagaaga
    28441 tggcgtgaat atctggggtg acggtagcac ctataaagga aacgatatcg aacgtttcta
    28501 tcgttatggt ctgctgacca atgctgaact gcagatttac aaaccgtggc ttgatactga
    28561 ctttattgat gaactgggcg gccgtcatga gatgtctgaa tttatgattg cctgcggttt
    28621 cgactacaaa atgtctgtcg aaaaagccta ctccacagac tccaacatgc ttggtgcaac
    28681 gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc aaaatcgtca acccgattat
    28741 gggcgtgaaa ttctgggatg agagcgtgaa gatcccggca gaagaagtca cagtacgctt
    28801 tgagcaaggt catccggtgg cgctgaacgg taaaaccttt agcgacgacg tagaaatgat
    28861 gctggaagct aaccgcatcg gcggtcgtca cggcctgggc atgagcgacc agattgagaa
    28921 ccgtatcatc gaagcgaaaa gccgtggtat ttacgaagct ccggggatgg cactgctgca
    28981 cattgcgtat gaacgtctgt tgaccggtat tcacaacgaa gacaccattg agcagtatca
    29041 cgcgcatggt cgtcagttgg gccgtctgct gtaccagggg cgttggtttg actcccaggc
    29101 gctgatgctg cgtgactctc tgcaacgctg ggttgccagc cagatcactg gtgaagttac
    29161 cctggagctg cgccgtggga acgattattc aatcctgaat accgtctcag agaacctgac
    29221 ctacaagcca gagcgtctga cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg
    29281 tattggtcaa ttgaccatgc gtaatctgga tatcactgat acccgagaga aactttttgg
    29341 ttatgccaaa actggcctgc tttcctcctc tgccacttca ggcctgccgc aggtggagaa
    29401 tatggaaaac aaaggccagt aattcgacat caaccctgcc cttcaggggt ggggttacct
    29461 tgcttatcct ctgtaaactc ctttctgagc atcacataat cgaaaacatc atggtttgca
    29521 tccacaaccc gtgagtctcc tttatagtgt gcaatcactt cctcaagcgt ttgcggctgt
    29581 tccggctttg ttacccccat ccacgcatta ataaggtaat tattgtaagc tgtcgaaaag
    29641 atattgtttt ccgttgtgcg atccacgcca tccccaagaa cggggctata ccagataaac
    29701 atcgggacat gatatgcctg ctggctagcc tccctgcctc catgaaaata gacgttcttc
    29761 ttcgtagggt cacgttccag gccgtggtcg gcaaaataca tgaccgaggc gcggcgatct
    29821 tttaataatt caaaaacctg acctagcaaa ctatctgtgt aatgaatgga gttgtcatag
    29881 caggcatcct gatcgtcctg cggttgaaac acggcgctgg attgcggata ggcgctacaa
    29941 gccggttcat ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc
    30001 tgtaatgctt gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt
    30061 tccatggcgc gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc
    30121 gagcttagcc agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg
    30181 taattatgaa tgtcatgact caaaacagaa tcagcagtta aagaaagggg aaccgacagc
    30241 gcagtgtaag gtgcgccgct tattgcttga ttaaacagtt tgatctgttt tctttgtgct
    30301 tcaacttgcg gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca
    30361 gactccccca caatcaacac gtaggtatca atacttgtat ccctgactga taattgaaaa
    30421 tacggcaccg tgtttgcaat tgagagtaat ctttgatgcg ctttcgctgc taaagcaaaa
    30481 tagttgagat tgaaaaacgg cgtataggta gcaaatcacg acgctagtat atatggactg
    30541 aacgcttttt tattttttgc atctttataa gcaaattgac aagcggaaaa taaactgccc
    30601 gaaatgacaa tcagcaataa tattcctgtc actttttttg tcggcaagga aacatcatat
    30661 tttattatta ctgccaaaaa aagaagggat aaaaaagcaa aagcacatag ataagggata
    30721 tacatcccca gcattttcac aacttcatcc ggatcactct gcagcacact aatcgcaaat
    30781 ccatcattga aagttgtacc aaaggtacac catgaataga ggctaatact catgtctgcc
    30841 gcagtcagta acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt
    30901 ttaacaacca ggatggtcag gacgaagaac agcaatcgct gaaccatatg cgctccggaa
    30961 gaggcgacta agttcgtaat accaaaaaca ataacacaaa gatacaacaa ccaatgagat
    31021 ttaaaatctt ctagcaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
    31081 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgatcca
    31141 tatcagaccg aaacactgtt agttttttct ttttaaatga tgcgaccatt gcgttctcag
    31201 taattaaata acattatcaa tgcgtctcta aacattacgc agaacgtagc catcagtagt
    31261 tatactgaca ggagcaggat ggtttatcga gaagtaaaca tatccgtatt cgtaccggaa
    31321 ctggagttga cacaataaag tgccaattat gtcagcagta gggaaaagtt tataactaag
    31381 gcgtaccggc accatcgttt caaggtacca gctacgagta aagcaactgg acgagataca
    31441 gatacctgac aaccattcat cagaccagga ccaaaacgaa aagagacgct tttcagcgtc
    31501 tcttttctgg aatttggtac cgaggacggg acttgaaccc gtaagcccta ttgggcacta
    31561 ccacctcaag gtagcgtgtc taccaattcc accacctcgg cacggatact actttttagt
    31621 tcgggatatc gctggtcggc ttagccggag cagctggctg agtttgttcg gttttcgccg
    31681 gtgcactcag attttcccat tcgctacctc tattggtttt gttgctattg atgttaccca
    31741 gcaccagact gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg gtcatgaagt
    31801 taccagaacc acttgaacca aacagcgtag cggaagcgcc tgctccgaag gaggctccca
    31861 tatcagcgcc tttaccttgc tgcagcatga tcagaccaac aaggccaatt gccacaataa
    31921 ggaaaactac taaaagagct tcgtacataa tcaacctgtt ccttgcggag ttgccgcgta
    31981 ccaatgcttc aaccaataaa gcgggagttt ttaatctttc ccactgaagc gggtgtgaat
    32041 actaaccaaa acgaatgacc ttcgcaaggg caattttatc gcattgtatc aactgcggaa
    32101 aaaaacagca aaaccgatgt gttcgccgag aaaaaaggcg acaatcgccg cctttttagc
    32161 cagttatcta acgctttaaa cggcttttac tgcatcggcg atgcggtgtg caaattcagt
    32221 cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg tgccggattt
    32281 acgcagcaac acgcgtccac ggttgcccag cgcagcttca acctctgcgg tcacggcttt
    32341 aactgactca tgctcaagcg gatcgccgct acctgcggtg taacgtacgt taaccagaat
    32401 ctgcgggaac attttcatgc cgctgcaaag gtcgtgcagg ctcatatggt tacgtgccat
    32461 cgccgccagc acctgcaagc cagcaacgat gccgtcaccg gtagtggttt tatccagcag
    32521 gatcacatga ccggaattct ctgcaccgat acgccagcct ttctcctgca ttttttccag
    32581 tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt tcagcgccag
    32641 ttcaagcccc atgttgctca tcaatgtacc cacagcacca ccacgcagct ggccctgacg
    32701 aagaccttca cgcgcgatga tatacatgat ctgatcgcca tcgactttat tgccttcatg
    32761 gtcaaccata atcacgcgat cgccgtcgcc gtcgaaggca ataccgagat ccgctttttc
    32821 agccagcaca cgagcctgga gcgcgcgaac gtcggtagcc cccacttcgg cattgatgtt
    32881 tacaccgttt ggctcacaac cgatagcgat aacgttcgcc cccagttcgc gcagcacgtt
    32941 cggcgcgatg tgataagtcg caccgtttgc acaatccacc acaatcttca gttcactgag
    33001 gctaagttcg ttcgggaacg tggctttgca aaactcgata tagcgacccg cggcatcaac
    33061 gatacggctg gctttaccca gttcagccga atcaacgcag ctgatctcct tttccatttc
    33121 cgcttcgatg gcctcttcta ccgcatccgg cagtttagtg ccgtcgatag agaagaattt
    33181 aatcccatta tcgtagaacg ggttatgcga tgcagatatc acaattccgg cctctgcgcg
    33241 gaaggtacgc gtcagataag ccacggccgg tgttggcatc gggccagtga agagtgcgga
    33301 aaggcccgct gccgccagac ccgcttccag tgctgactcc agcatatagc cagaaatacg
    33361 cgtgtcttta ccaataataa tcttacggga gccgtggcgc gccagcactt tacccgcggc
    33421 ccaacccagc ttaagcacaa aatcaggtgt gatcggcgca tcccctacac gaccacgaat
    33481 cccatcggta ccgaaatatt tacgattact catagcgttt gttttccttt gcagacagag
    33541 tggcttccac cacccgcatc gcttctacgg tttctttgac gtcatgaaca cgaatgatgt
    33601 gcgcgccttg cattgcggca atgaccgcac aggccagact accgctcagg cgctcggacg
    33661 gccccacgtt cagcagctgc ccaatcatcg attttcgtga catacccacc aacagcggca
    33721 ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga atagttatgg gagagatttt
    33781 taccgaaacc gaatccgggg tcgagcaaca atttctcttt tgcgataccc gcctgctcgc
    33841 aacgtgctat ttgctcaata aagtagcgat tcacttctgc aaagacatcg tcatacttcg
    33901 gagcttcctg catggttttt ggatttccct gcatatgcat cagacaaacc ggtaaaccgg
    33961 tttctgcagc cgcctccaga gcgccaggtt cggaaaggga gcggatatca ttaataatgt
    34021 gagcgccaac tttcgctgac tcacggatga cttctggttt ggatgtatcg accgagatcc
    34081 agacttcgaa gcgttgagca attgcctcaa ccacaggaat aacacgttgc aactcttctt
    34141 caacgctaac ttccgccgcc cctgggcgcg tggactcgcc accaacgtca atgatcgtcg
    34201 cgccagcatt gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag ttatgcgtgc
    34261 caccatccga aaaggaatca ggcgtgacgt tgaggatccc cattacgtga ggatggctaa
    34321 ggtccagtga agtaccctgg gcaaagagtt tcattatgtt atccctggta tgaattgata
    34381 agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa atacagtcat ctgatgcggg
    34441 aacttacttg tcgcctaact gctctgacat ggtgttaccc gggttcggcg tacgcggttc
    34501 atcaaccgga cgaggagcct ttggactacc attgtcgcca gaattgttag aagcgcctgg
    34561 ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat caatctgcgg
    34621 tgcgtcgata gtctcatatt tcatgagagc atctttcatc gcatgcagaa tatccatatt
    34681 gtcggtcaga agctgacgcg cacgattata gttacgctca atcagtgctt tcacttcctg
    34741 gtcgatgata cgtgcagttt catcggacat atgtttcgct ttcgctacgc tacggccgag
    34801 gaatacttca ccttcttctt ccgcgtacag cagtggaccc aatttctcag agaagcccca
    34861 ctgagtcacc atgttacgtg ccaggttggt cgcaacttta atatcgttgg acgcaccggt
    34921 agaaacatgt tccggcccgt agatgatctc ttctgccaga cgaccaccgt acagcgtaga
    34981 aatctggctt tccagtttct gacggctggc gctgattgcg tcgccctcag gcaagaagaa
    35041 agtcacaccc agcgcacgac cgcgtgggat aatcgtcact ttatgcaccg gatcgtgttc
    35101 cggcaccagg cgaccgataa tcgcatgacc cgcttcgtgg taagccgttg attctttctg
    35161 cgcttccgtc atcaccatgg agcgacgttc cgcacccatc atgattttgt ctttcgcttt
    35221 ctcgaactca accatcgaca caacgcgttt gttgccacga gcagcgaaca gtgccgcttc
    35281 gttcaccagg ttcgccaggt cagcaccgga gaaaccagga gtaccacgag caatgattgc
    35341 cgcgtcgata tcgggtgcca atggtacgcg acgcatgtga actttcagga tctgctcacg
    35401 accgcgaaca tctggcaagc cgaccacaac ctggcggtcg aaacggccag gacgcagcag
    35461 ggccgggtcg agaacgtccg gacggttagt cgcggcgata acgatgatac cttcgttacc
    35521 ttcgaagcca tccatctcaa ccagcatctg gttcagagtc tgttcacgtt catcgtgacc
    35581 accgcccaga ccagcgccac gctggcggcc tacggcgtcg atttcatcga taaagatgat
    35641 gcacggtgcc gctttcttcg cctgttcgaa catgtcacga acacgggatg cacccacacc
    35701 gacgaacatt tctacgaagt cagaaccgga gatagtaaag aacggaactt tcgcttcgcc
    35761 tgcaatcgct ttcgccagca acgttttacc ggtacccgga ggaccgacca tcaggacgcc
    35821 tttcgggatc ttaccgccga gtttctggaa gcggctcggc tcgcgcagat actctaccag
    35881 ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg tcgttttgat
    35941 ctgatcttcc gtcagcatgc gcgctttgct cttaccaaac gacatggcac ctttgccacc
    36001 gccgccctgc atttgacgca tgaagaagat ccagacacca atcagcaaca gcatcgggaa
    36061 ccaggagatg aagatagaag ccagcaggct tggttcttca ggcggttcac cgacaacctt
    36121 gacgttcttg gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa tgtaagtggt
    36181 ataacggtta ctatctttct tggtaacgtt gatttcacgt ccgttgatac gcgcttcacg
    36241 aacctggtcg ttattgacct cttgtaggaa ggtagagtaa tccaccttac ggccattaga
    36301 ctcgctgggc ccaaagctct ggaatactga catcagcaca acggcaatga ccagccagag
    36361 tattaggttt ttcgccatgt cactcaaggg attaacctct tattacaact gtgttaaaaa
    36421 cagcgtcagg atactctata tccagcatct ttcaaacttt cgtctgaaat ctcccggtta
    36481 gggtttacgc ccggtcgcta caatatacac ttctcgcgaa cgtgcacgag aagagtccgg
    36541 cttacgaact ttgaccttcg taaacaggga gcgaatttcc cttagatact catcgaaacc
    36601 ttcgccctgg aacaccttca ctacaaaact gccacctggc gctaatacat cacgacacat
    36661 ttctagcgcc agttccacca gatacatggc tcgggggata tccaccgccg gtgttccgct
    36721 catgtttggt gccatatcgg acatgacaac ctggactttg ctgtcgccaa cgcgctccag
    36781 cagtgctttc atcaccagtt catcacgaaa atcgccctga agaaagtcca caccaacgat
    36841 aggatccata ggtaaaagat cgcaagcgat gatgcggcct ttgccgccaa tttgggtgac
    36901 cacatattgt gaccaaccac ccggagcagc accaaggtcg acaaccgtca ttcccggttt
    36961 aaagagtttg tcactttgct gtatttcatc aagtttaaac caggcacggg aacgtaatcc
    37021 ctttttctgt gcctgttgaa catatttatc gctaaagtgt tcctgaagcc agcggctgga
    37081 gctggcagaa cgcttcttac ctgtcattta actttcccat ggggataact catcgtaacc
    37141 aattgcgtaa atttttacgc gcctatttgg tgatatatgg gagatggcgg tagaatgacc
    37201 cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa caaaaacagc
    37261 acctgaaagg tctggcacat ccgctcaagc cagttgttct gcttggcagt aatggtttga
    37321 ccgaaggggt gctggccgag attgaacaag cgttagagca ccatgaactc atcaaggtga
    37381 aaatcgccac cgaagatcgc gaaacaaaaa ccttgatcgt ggaagctatc gtgcgcgaaa
    37441 ccggcgcctg taatgtacag gtcatcggta aaacgctggt gctttatcgc ccaactaaag
    37501 aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt tacacacatt tgctgtgtaa
    37561 aacgaggggt tttccgcagg caggagagca aaatgccacg ctctgttcgt tgataaaagg
    37621 ccgcagagcg gcctttttcc tttctttaca atacatcaac atcttgagta ttgggtaatt
    37681 cttacaggta ttccacctta attacttcaa attctacttc gccgcccggc gttttgatga
    37741 ccacaacatc atcttcttct ttgccgatca ggccacgagc aataggcgag tttacagaaa
    37801 tcaggttttg tttaaagtca gcttcgtcat cgccaacgat gcgataagtc tgttcttcgt
    37861 cagaatccag attcagcacc gttacggtag caccaaaaat aacgcgccca ttgttgggca
    37921 ttttggtgac atcaatcacc tgcgcgtttg acagcttggc ttcgatgtct ttgatacggc
    37981 cttcgcagaa gccctgctgt tcacgagctg cgtggtattc ggcgttttct ttcaggtcgc
    38041 catgctcacg cgcttccgcg atagcagcaa tgatttcagg acggcgcaca gatttcagaa
    38101 aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata gcttgcattt
    38161 gttatacctc ttgaatattc ctgatggggc aagtcttcac ccaccccggc tgttcggcct
    38221 gcccggcata actcctcacc gggaccagaa gcaaaaaaat accgacccgg gtacaagtcc
    38281 caggtcagct acaattcaca ttttgatggt cattttaccc tgaagttccc gaagggtcat
    38341 cgtttacttt atagggcgtt gcgccgtagt atgacggctc gattccaggt tgttagcgcg
    38401 agattatgcg attttccaga tttatcatcg gattgaccag ctgtatagcg ttcagtgttc
    38461 aggccgcgaa tgttgatgag tacgttactc aactccccgc aggtgccaac cttgccctga
    38521 tggtgcaaaa agtcggtgcg tcggcccccg ctattgatta ccacagtcag cagatggcgc
    38581 tgcccgccag tacccagaaa gtgattactg cgctggcggc gttgattcaa ctcggccccg
    38641 attttcgttt caccacgacg cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg
    38701 gtgacttagt ggcgcgattt ggtgccgatc cgacgttaaa acgtcaggat attcgcaata
    38761 tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg ttgatagata
    38821 cctccatttt cgccagccac gataaagccc ccggctggcc atggaatgac atgacacaat
    38881 gctttagcgc tccgcctgct gccgccatag ttgaccgcaa ctgtttctcc gtctcgctct
    38941 acagtgcccc aaagcctggt gatatggctt ttatacgcgt ggcatcttat taccccgtta
    39001 cgatgttcag ccaggtacgc accctccccc gtggttctgc cgaagcgcaa tactgcgaac
    39061 tggatgtggt gccaagcgac ctgaaccgct ttacgctgac gggatgcctg ccacaacgtt
    39121 ctgagccgct cccgttggct tttgccgtgc aggatggagc cagctatgcc ggtgcaattc
    39181 tgaaagatga gttaaaacag gcgggtatca cctggagcgg aacactgctg cgccagactc
    39241 aggttaacga acctggaacg gtagttgcca gtaaacagtc ggccccgctg cacgatctgc
    39301 ttaagattat gctgaaaaag tcggacaaca tgatcgccga tacggttttc cgcatgatcg
    39361 gccatgcgcg cttcaatgtg cctggaacat ggcgggctgg gtcggacgcc gtgcgtcaga
    39421 tcctgcgcca gcaagccggt gtcgatattg gtaacaccat tattgccgat ggttcagggc
    39481 tttcgcggca taacctgatt gcccccgcca ccatgatgca ggtgctgcaa tacattgccc
    39541 aacacgacaa tgaacttaac tttatctcca tgctgccgct tgcgggctat gacggctctt
    39601 tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg caaagtctca gcgaaaaccg
    39661 gttcgttgca gggggtatat aacctggcgg gattcattac cacagcgagc gggcaacgaa
    39721 tggcatttgt acaatatctt tctggctatg cagtaaaacc cgcggatcag cgtaatcgcc
    39781 gtattccgtt agtgcgtttt gaaagccgtt tgtacaaaga tatttatcag aacaattagt
    39841 caaaaagaaa ccccggcgca tggccggggt ttcagattat tgacaaagtg cgctttgttt
    39901 atgccggatg cggcgtgaac gccttatccg gcctacaaaa tcgtgcaaat tcaatatatt
    39961 gcaatcatct tgtaggcctg ataagtgtag cgcatcaggc ttatttggca ttggtcatta
    40021 gtgattaacg cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc cagtcgtcat
    40081 cccagtcttc atcatcctct tcagcaatct cttcaagctg ctgacggtga taatcatccc
    40141 acatgaattc gactttctct ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa
    40201 tgataaaggt catcacatcc cagcagagat ctttcacgcc cagtccgctc gccgcagaga
    40261 tcagataata tttatcttcc cagcccagcg cttcagcgat cgctttcgct ttctcttcgg
    40321 cttctgcctt atccagcaga tcgatcttgt tgaagactaa ccaacgcggt ttcgccgcca
    40381 gatcctggct gtatttttcc agctcgctga taataatacg cgcgttttca accggatcgg
    40441 tgccgtcaat cggatcgata tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct
    40501 tcaggaagcg aatgcccaga cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat
    40561 cggcaacaac gaagctcttt tcgttgtcca ttcgtaccac acccagactt ggcaccagag
    40621 tggtaaacgg ataatccgcc actttcggtt tagccgccga taccgcacga ataaaggtcg
    40681 atttacccgc gtttggcatc cccaacatac cgacgtcagc cagcagcatc agctccagca
    40741 gcagctcacg cttatcgccc ggcgtaccgt tagttttctg ccgtggtgta cggttaactg
    40801 acgatttgaa acgggtattg cccagaccgt gccagccgcc cttagcaacc agcagacgct
    40861 gaccgtgttt agtcatatca cccatggttt caccagtacc ctggtcgatt acacgcgtac
    40921 ctaccggcac tttaatcgtc acgtctttac cgcgcttacc ggtacaatcg cggcttgcgc
    40981 cgttctgacc gcgctctgca cggaaagatt tttcaaaacg ataatcgata agcgtgttca
    41041 ggttctcgtc ggcttccatc catacgtcac caccatcacc gccgtcgccg ccatccgggc
    41101 cgcctttcgg aatatacttt tcgcggcgga agctcacgca accattaccg ccatcacctg
    41161 caacgaccag aatcgatgct tcatcaacaa acttcatttt attctccgta aatcattcgc
    41221 ctgcgcgggg ttgcgatacc accgttgtat gcttacgtaa tccgccccaa atacgatgac
    41281 caatggcgga atacatcgcg cccgcaacca cgacaaacgc accgagataa cctaaaaggt
    41341 ttaacatcgg tctggcgaag aaatcgggcc aggccagtga taaaagatct gaaaaaaaca
    41401 gcgtaaacag tggggtgagc gtgatgatcg cgctcacctg cgctgcctgc cagcgagcca
    41461 tcgcttccgc caaggcgcca tatcctacca aggtattcag tccgcaaaaa attaaacatg
    41521 cgagctgcca gtggctaagc tgcgctatca ctccaggctt tgccagaggg aagagcgcga
    41581 ttgtacataa agtgtacagt aaaaacagga tctgcggtga ggccagccga cgcaataaaa
    41641 ccttttgcgc cacgccataa ctcacccaaa ccgtcgccgc accgacccca aagataactc
    41701 cccaggtgta atcggtgagc tttgtaaata tctcgaccag actggtgtta aaaaacatca
    41761 ccagaccgct caggagcatc aatgccccta caacctgagt gctgcgcatt ttctctttca
    41821 ggataaatac gctggcaacc atcatgccaa ctggcgagag ttgcccaatc acctgcgaag
    41881 cggtcggact caggtattgc aaggatgagc tgaacaggat gaagttccca aacagcccgg
    41941 cggtcgccac tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt aatggcggca
    42001 acctcttctt caccgcaaga atggcaccaa ggccaatact cgccatcaag aaacggtaaa
    42061 acacgattgt cggaggttcc atcacctcca gcacctgctt cattgcgatt ggcaacgccc
    42121 cccagcaaat tgctgtggta agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac
    42181 cgtattccct gcaaagcgca tttgcggatt aacgtcgcac catgcgcatt tttaccggtt
    42241 atcgaatgta aaaagccccg caacgtgttg cggggctttc atccgttacc gggacgcgaa
    42301 aaacttattc agcttcgatg ctgataaatt tacggttttt cgggccttta acttcgaatt
    42361 tcactttacc gtctgcttta gcaaacagag tgtggtcacg accgcaacct acgttagcgc
    42421 cagcgtggaa tttggtacca cgttgacgaa cgatgatgct acccgccaga acggattcgc
    42481 caccgaaacg cttaacgccc aagcgtttag cttctgaatc gcgaccgtta cgtgtggagc
    42541 cgccagcctt tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc tgatgccagt
    42601 aattttcaca tcagtgaacc actgacgatg gccctgctgc ttacgatagt gtttacgacg
    42661 acgaaactta acgattttaa ctttctcgcc acgaccgtga gcaacaactt cagctttgat
    42721 tacgccgcca tcaacgaaag gaacgccgat tttgacttct tcaccgtttg cgatcatcag
    42781 cacttcagcg aactcaacag tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt
    42841 ctgaccttcg cttactcggt gttgtttacc accactttgg aaaaccgcgt acatataaaa
    42901 ctccgcttcc gcgcacacct tttcaatgat tcagagtgcg ctataaatat tcacaatagg
    42961 gcgcgaatat tacgcaaaac gcacgccttt gacaagtgct acagtcaata cacgaagaaa
    43021 aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa ttaccctata
    43081 ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc
    43141 cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg
    43201 ttaatgcggc aatccttgag cagcttaatt ccgacgtcca actgatcaat cagttaggct
    43261 attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac
    43321 gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc
    43381 acacggcgac tctgctacac gacgacgttg tggatgaatc agatatgcgc aggggtaaag
    43441 ctaccgccaa cgccgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata
    43501 cccgcgcgtt ccagatgatg accagcctcg gttcactcaa agtgctggaa gtcatgtcag
    43561 aagctgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg
    43621 acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg
    43681 ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaggaggag aaaggcctgc
    43741 aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca
    43801 atgccgatgg cgaacagtta ggtaaaaacg tcggcgacga tctgaacgaa ggtaaaccga
    43861 cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta
    43921 ccgccatcga acaaggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg
    43981 cttgtggatc tcttgaatgg acgcgtcagc gcgccgagga agaagcagac aaagccatcg
    44041 cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca
    44101 tcgctgttca acgcgatcgt taatcccctc ccttcatccc gcgcagtgcg cgggatgatt
    44161 ccagtaaatt ccataaaaac actaattcag cgctataaca cctgaaaaaa gggcgatgaa
    44221 tctttatata ttctgaatat tcacactctt tacaggaact ttttagcgca atagaccatc
    44281 aggagtatag tgatgcccga cagaagaagt gttctgaatg aaagcgacta acttaaggag
    44341 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcaggtttg
    44401 cgcaagaaag gaacgtcaat ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg
    44461 ctggcaaacg cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg
    44521 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    44581 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    44641 tagccccgcg aacggggctg ccagctctca gacgattact cgcctttcac acgctcaata
    44701 tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    44761 atacgatcaa ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat
    44821 gcacgcagat cggttgccat aacctgtgca ccagaaagtt tttcaacacc atgacaaata
    44881 acggtattgc tttcgatttc ggcgtgcgcg cccatacggc tcagctctgg cacatgcata
    44941 aagcggtttt caaagaccgt ttcggtgatg aacccggtcc cttcagccac caggttcaac
    45001 agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta
    45061 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    45121 tccgctccag catcacgcag tttcgccagc acggcgtcca gagtatctgg ctgcgcgtta
    45181 cggcagataa ttttgccacg agagatcgcc gccgccacca ggaaagtacc ggtttcgata
    45241 cgatccggca gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    45301 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    45361 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg tgccttccgc cagggttgca
    45421 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    45481 ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    45541 aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca
    45601 ggtagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagcgc ccagatagaa
    45661 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    45721 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag tttcattgat
    45781 gtatcgacgt ctttcagttt cgggacgttc tggatctcta ccggttcttc cgccagtagt
    45841 gcggcaaaaa gaataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    45901 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    45961 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    46021 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    46081 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatacc gtcaaacaac
    46141 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    46201 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    46261 agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    46321 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    46381 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    46441 ttcgcgaggg tatacacttt gtcgtttacc ccctgaagcg tcacgttgtt gccctgcttt
    46501 ttcgccagat cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca
    46561 cggctaagat cgatgcaggt aatccccttc accgcctcct cacgcatttc ccaaagcggt
    46621 agcagaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    46681 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    46741 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    46801 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcttgc cagtttcccg
    46861 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cggacggcca ttcgggtcaa
    46921 taatggtaac gcgaataggc acaatggttt tatcgcccag cggctgttct ggcgcaatct
    46981 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    47041 ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca
    47101 gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg
    47161 tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    47221 ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg
    47281 cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt
    47341 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    47401 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    47461 caatgagatc ttccagcacc atcgcagact tggtgtcctg aattgtatcg ccatccttca
    47521 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttctc
    47581 ccagcaggcc ggaagtacga atgctcagcg aactggtatc aggaatgtgg ttataacgtt
    47641 gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca
    47701 cccggcccac aacaacacca ccaatgctga ccggagagcg cgctttcagg ccgccaatgt
    47761 tatcgaacgt cgcataaagc gtgtaggtcg attcagtacg tatggacgtc acgttcgccg
    47821 ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa
    47881 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    47941 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    48001 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    48061 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    48121 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagcgat
    48181 ccgccccaga tccccacggc gacgaagata accgtcaaca gtggtaatga aataacccca
    48241 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    48301 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttaacgccga accagcacgc
    48361 ccggcaaaca acagcgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    48421 agcataccca gactggtttc cgcgctataa gtggtcagaa ccagataacc ttgcagcccc
    48481 aacaccattc cgatgaacac gccagaaact acaataatca gcatcgacag gacgccgaca
    48541 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    48601 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    48661 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    48721 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    48781 ctgacgtacg cgcggatcag gattcgtctg caacgcctgg gcactgccat gagcgacaat
    48841 ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg
    48901 agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag
    48961 tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg
    49021 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    49081 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    49141 atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa
    49201 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttacgcac
    49261 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc
    49321 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    49381 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    49441 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    49501 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    49561 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    49621 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag
    49681 cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa
    49741 ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc
    49801 tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta
    49861 catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag
    49921 ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg
    49981 cgctggttcg tccttttacc gtccattccg atgttctgcg ccgtgaatta cccttaatgt
    50041 tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgaagcgatg
    50101 gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc
    50161 aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgac ggcggattgc
    50221 ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat gccagtggcc acgcggatgg
    50281 tggttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg acgatggctc
    50341 tgacggcaat tgctatcgga accagtctgc cggaactggc aaccgcaata gcgggggttc
    50401 gcaaaggtga aaacgacatt gctgtcggaa atatcattgg cgcaaacatt tttaatattg
    50461 tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca ctggcgtaca
    50521 gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc
    50581 gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt atcgtatggc
    50641 tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg
    50701 cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt
    50761 gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcctgt
    50821 gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac
    50881 atcgggcgca aaatggccgc aacgtttgcc agcaccggta caccttcatt tttcgtccat
    50941 cctagtgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt ggtgattgct
    51001 atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt
    51061 cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat
    51121 gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc
    51181 agcaccaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc
    51241 ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt
    51301 ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg
    51361 gccagtctgc gtgacgcatt gctggaagtt acccgcaaaa atcttggtat gactgtcatt
    51421 tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc
    51481 gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata
    51541 cgtgtgcgcc ctggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc
    51601 acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta
    51661 ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt
    51721 cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca gagaacattc
    51781 gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat atgggcaata
    51841 acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca
    51901 cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt
    51961 gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg atcgccttta
    52021 gcgatttgct ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtt ggcgatgatc
    52081 tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc
    52141 cactgttgat cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc
    52201 gcgaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat
    52261 cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg ttctggtgat
    52321 gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga
    52381 tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag
    52441 ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac
    52501 gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga
    52561 taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaacgc
    52621 acttgtgcca gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt
    52681 gacgcaggat gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag
    52741 cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag
    52801 aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac
    52861 ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca
    52921 ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg
    52981 tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt acccgtccgg
    53041 gcggcgaaca aggtaaagaa gtgattgacg gatacggtaa accggcaacg ttctaccaga
    53101 tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac gaactggcaa
    53161 aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc aacattaagg
    53221 gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc gacaaaggca
    53281 agcgcgtaac caccgttctg gtgccgtcgc agctgcagga caaaaacaac aaaggccaga
    53341 ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg
    53401 caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg
    53461 aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc tacatggttg
    53521 taggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc
    53581 tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccacaggaa gcctccattt
    53641 tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt gacgacttgt
    53701 ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga agagtttcac attgagcacc
    53761 tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc
    53821 gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc ggggttgacc
    53881 cgatctcggt tatcgacatt aaacgcatca ttgaacacct gcgcgacagc ggcctgggcg
    53941 tgctgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg
    54001 tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa gacgaacacg
    54061 ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt
    54121 ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca
    54181 actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt cgacgctgga
    54241 acttcagcag gagctacagc aggcgctgga gagtaatccg ctgcttgagc aaatcgacac
    54301 tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acgctggaca ccgccgacgc
    54361 gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta
    54421 caccgctggt acaccatccg gcaccagcgg tgactacatt gacgacgagc tgccggtcta
    54481 ccagggcgaa acgacgcaga ccttgcagga ttacctgatg tggcaggtcg agctgacacc
    54541 gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgaaaccgg
    54601 ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agatcgacat
    54661 cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt gacccggtcg gtgtagcggc
    54721 aaaagatctg cgtgactgcc tgctaatcca actctcccaa ttcgataaga ccacgccgtg
    54781 gctggaagag gccagattga tcattagcga tcatctcgat ctgttagcca atcacgactt
    54841 ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct
    54901 gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt
    54961 cattccagat gtgctggtgc gtaagcataa cggtcactgg acggtagaac tcaacagtga
    55021 cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa
    55081 cgatggtgac agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag
    55141 tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca
    55201 agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg atatcgccca
    55261 ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag
    55321 tccacgaggc atttttgaac tgaagtattt cttttccagt cacgtcaata ccgagggcgg
    55381 cggcgaagct tcctccacgg cgattcgtgc actggtgaag aaattaatcg cggcggaaaa
    55441 cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat
    55501 ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca
    55561 gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg
    55621 gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagctaaa tttgccaaac
    55681 ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg gagaaagtca
    55741 cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag
    55801 gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca
    55861 aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc
    55921 ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt
    55981 gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca
    56041 gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc
    56101 tggaaatcat cagcgagctg gcggcgaaac aacttagcct gccgcctcag gtggtttttg
    56161 aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc
    56221 cgcatggcaa acttgaagaa gatactctgc gtgccgttgg cgttttcgtt cagctcgaaa
    56281 cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt gccctgctgg
    56341 tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg aaacgtctgg
    56401 cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa
    56461 tcattacgga taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta
    56521 gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag
    56581 gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc
    56641 ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgcgagatt tctgccgccg
    56701 tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta
    56761 acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt aataccttaa
    56821 ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg
    56881 aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga gcggatctga
    56941 ttgtcgatac ctcagaaatg tccgttcacg agctggctga aatgctgcgt acccgtctgc
    57001 tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc aaacacggta
    57061 tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc
    57121 cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca
    57181 cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc
    57241 tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc gggaagcacc
    57301 gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc
    57361 agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat
    57421 cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg
    57481 ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt
    57541 tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg aagcgaccgg
    57601 tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaattctg gttttgatga
    57661 agattaatct tcatcacttt gacatacaac tcccttcaag ctcccccgac aataagaaaa
    57721 tcacgtactg aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt
    57781 cagaagaacc ttaagaaaac cttaagaggc attgtttagg ttttgtttaa gttaatcgac
    57841 catactggag atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa
    57901 agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc
    57961 tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta
    58021 aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac
    58081 ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc cgattttgac
    58141 ggcaagcctt ccattacgct gaccgaattt gctgaacaat gccgttacga agaagacatt
    58201 gcacaactgc gccagctact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg
    58261 acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc
    58321 ccggtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag
    58381 tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg
    58441 gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg
    58501 cgcgaagctt aatccagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc
    58561 tgccgtaaaa tccacgcctg acggctacgc acgtagcccg atggcgagga gactttgaaa
    58621 cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc
    58681 gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat
    58741 tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc
    58801 agcgttaatc cagcttccag cccttttcgt acccagctac gcccatccca taaaaagaga
    58861 ttttggccgt ctgctgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcgt
    58921 tgtgcgccag ggctttctca atggaagcga catcaaagcc ccagtgctca ggaaatttct
    58981 gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc
    59041 agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc tgtcgctcga
    59101 ccattaccgc tgagaaggga acaggcgcaa cgctaaacaa cgcgatgccc ccgccccaga
    59161 aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta aacaccgtta
    59221 agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct
    59281 cgccgcttct gcaatgttct gcgccagcat atatgctggg gtggtgacaa tcttattgtc
    59341 ttcatcaacc acgatatcat cgacaggaca cggcacatgc tccgcgccca tctcttccag
    59401 cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt
    59461 cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct gatgcatcgc
    59521 ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg ctaccaagac tggcaaaatt
    59581 gcttaaattc ttcgccgcgc caaacccccc cggcacaatc aacgcatcca gttcagcggc
    59641 atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac
    59701 attgcgcgtt tccgtcatcg cttcgccagt taaatggttg ataacatcaa cctgctgctt
    59761 atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac
    59821 cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt
    59881 cttcattgtg atcatccttt cgcaattgac tgaaacacat taattttttt aataaaaatg
    59941 gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac
    60001 tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa tttacggcgc
    60061 agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt
    60121 ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc cactcttctt
    60181 tcatctcttc aatccattca ccgacgttat cttcccaggc gggaaggtca ggagactgaa
    60241 tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca cctttaattt
    60301 tatgtccttc ctcaacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgc
    60361 tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc
    60421 ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt
    60481 tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca
    60541 tcgcggttag cgccggaacc gaaagcggct tactcagcac atcatccatt ccagcattga
    60601 ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct
    60661 cgcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata tctggcaact
    60721 gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc
    60781 cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc gcgacaatca
    60841 cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt
    60901 catcaaacgc atcatcgacc tcttctgcca ccgacggtgc gtggatcgtc aacgtaaagg
    60961 ttgaaccttt gccctgttca ctggtaaccg taatatcgcc gcccatattt ttcgccagac
    61021 gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt
    61081 tcacctggta atacatggcg aagattttat ccagctcatc ctgcggaatg ccgataccag
    61141 aatcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct
    61201 ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc tgccgtaaac
    61261 gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt aggctccagg ttaaagcgca
    61321 atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga
    61381 aatcaaccgg ctgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa
    61441 tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatagttttg agatattttt
    61501 cctgctcggc ggtgagttcg gtatccagca gaatgcggct caggccgacg ataccgttca
    61561 gcggtgtacg caattcgtga ctgatggtgg aaataaacgt cgttttgtcg cggctggcgc
    61621 gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac
    61681 cgtgacgttt acccacgcgg tcgtagtacg gcactttacg gatttcaaag caggctttgc
    61741 gcccgtccgg gtaatccagc cactgttcat aggtcagtga cacattatga cggaacactt
    61801 tttcatcggt ttcaatgact tttgcggcgg cttccggtga gtaaacatca gcaggtttca
    61861 ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg
    61921 aaaactcttt atcttcgtta cgataaaaaa ccaagtcggg tgaagcatca aggaaggaac
    61981 gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa
    62041 ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcaattt
    62101 cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc
    62161 gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc tccagttgct
    62221 cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag
    62281 aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc gccatttgta
    62341 ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta
    62401 acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat
    62461 tccttcacga caacctgccc caataatacc caattctgac agttacgtta taaattgtgt
    62521 gagaaatgcg gaattcccct caacgaccta ctccgttgga ggtagccacg gacgtcccag
    62581 cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca ttcccgtcca
    62641 tcgattttcg caccacagcg gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg
    62701 gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtataatc
    62761 ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt
    62821 cacaatatgc agcggatgca gctttatgcc gtccacgctg gtttcaacca ctcgctccag
    62881 cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt gggaacacac
    62941 cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa aatcatggcc
    63001 gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct ccagccacac
    63061 ttcgtaaccc tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc
    63121 cgggcgggta ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat
    63181 agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt aggccagata
    63241 gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg
    63301 ctgctgcgct tcatcggcaa acgaggcaac attacagaat gtgcagccac cacgcccgat
    63361 ggtaccgtca cggttagggc agctaaaacc gccgtgcagc gtcagcttat gcaccttttg
    63421 cccataacga cgggtgagat caccaccaaa catattgact aatttctgta actgcataat
    63481 ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca attcggcgat
    63541 gacctggatc aatcgtcccg gcctgctttt atcaactgca taatcaatca aaattaccga
    63601 aatttcatgc acaatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc
    63661 cttatggatt ttaggcaaaa acagtagcat gaaacgtcat taccaattaa ggcagtataa
    63721 aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt tatgacagtc
    63781 aggaattgac tgtttctcta acgactctcc ttttagcctt aaagataaaa tctattttaa
    63841 tttcagctat ttaataaaga attttgcgct aaagcacatt tctgtaccaa taagcttgcc
    63901 atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag
    63961 cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg
    64021 cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc
    64081 acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg
    64141 aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga
    64201 tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg
    64261 cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac ggttgcggct
    64321 tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt
    64381 tagcaaaaaa ctacgctgtc gggatgctct tcctgaataa agatcctgaa ctcgccgctg
    64441 ccgcacgccg catcgttgaa gaagaactgc aacgcgaaac cttgtcgatt gtgggctggc
    64501 gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccacgca
    64561 ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc acgcgatatg gagcgtcgtc
    64621 tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct
    64681 gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg gatctgccgc
    64741 gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catttgcctg ttccaccagc
    64801 gcttctccac taacaccgta ccgcgctggc cgctggcgca accgttccgc tatctggcgc
    64861 ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg cgtacctata
    64921 aattccagac gccgctgatc cctgacctgc acgacgccgc gccattcgtc aacgaaaccg
    64981 gttctgactc cagttccatg gataacatgc tggaactgct gctggcaggc gggatggata
    65041 tcatccgcgc catgcgcctg ttagtgccac ccgcctggca gaacaacccg gatatggacc
    65101 cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggtccgg
    65161 cgggcatcgt gatgtccgat ggtcgcttcg ctgcctgtaa cctcgaccgt aacggtctgc
    65221 gtccggcacg ctacgtcatt accaaagaca agctcatcac ctgcgcctct gaagtcggta
    65281 tctgggatta ccagcctgac gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga
    65341 tggtgatcga cactcgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga
    65401 aaagccgcca cccttataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg
    65461 aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg cttgccagct
    65521 accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc gtactgggcg
    65581 aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc gtgctctcca
    65641 gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg actaacccgc
    65701 caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa
    65761 tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa tcgccgattc
    65821 tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac cgcgcagata
    65881 cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacagtc aaagagctat
    65941 gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgtt gctggtgctc tccgaccgga
    66001 atatcgctaa agatcgcctg ccggttccgg ctccgatggc ggttggcgcg atccagaccc
    66061 gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc
    66121 gcgatccgca ccacttcgcc gtgttgctgg gtttcggcgc gacggctatt tatccgtacc
    66181 ttgcctatga aacgctgggc cgcctggtag acacccacgc gattgccaaa gattatcgta
    66241 ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa
    66301 tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctac
    66361 acgatgatgt agtgggcctg tgcttccagg gcgcggtcag ccgcattggt ggggcaagct
    66421 ttgaagactt ccagcaggat ctgctgaatc tgtcgaaacg tgcctggctg gcgcgcaagc
    66481 cgattagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac gcctacaacc
    66541 cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg cgagtacagc gactatcagg
    66601 aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta
    66661 cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac
    66721 gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg
    66781 cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc gaagacccgg
    66841 cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg
    66901 ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga
    66961 agccgggcga aggtggtcag ttgccgggcg acaaagtcac tccttacatc gccaaactgc
    67021 gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat atctactcta
    67081 tcgaagactt agcgcagctt attttcgacc tcaagcaggt taacccgaaa gcgatgatct
    67141 ccgttaagct ggtgtccgaa ccaggcgttg gcaccatcgc gactggcgtg gcgaaagctt
    67201 atgcggactt gatcaccatc gcgggctatg acggcggcac cggcgcaagc ccgctgtcgt
    67261 cagtgaaata tgcaggctgc ccgtgggaac tcggtctggt ggaaacccaa caggcgctgg
    67321 ttgccaacgg cttgcgtcat aaaattcgtt tgcaggttga tggcggcctg aaaacgggtg
    67381 tcgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc actggcccaa
    67441 tggtggcgct cggctgtaaa tatctgcgta tttgccacct gaacaactgc gccacgggtg
    67501 tagcaactca ggatgacaaa ctgcgtaaga atcactatca cggcctgccg ttcaaggtga
    67561 ctaattactt tgagtttatc gcccgtgaaa cccgcgagct gatggcacag ctgggagtaa
    67621 cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agagctggac ggtttcaccg
    67681 ccaaacagca gaaactggcg ctgtcgaagc tgctggagac tgccgagcca catgcaggta
    67741 agtcactcta ctgcactgaa aacaacccgc cgtttgataa cggcctactg aacgcgcagt
    67801 tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag caaaaccttc tggttcgata
    67861 tccgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg
    67921 gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc gcaggccaga
    67981 gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct gaccggggat gccaacgact
    68041 atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggttccgcct
    68101 tccgcagcca tgaagcaagc attatcggca acacctgcct gtatggcgcg accggtggtc
    68161 gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc ggtgctatca
    68221 ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggcggt attgtctgta
    68281 ttctgggtaa aaccggcgtt aacttcggcg cgggcatgac cggcggcttc gcttacgttc
    68341 tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga actggtcgaa gtcttaagcg
    68401 tcgacgatct ggcgatccat gaagagcatc tgcgcggtct tatcaccgag catgttcagc
    68461 ataccggttc tcagcgcggt gaagagattc tggcgaactg gtcaaccttc gccacgaaat
    68521 tcgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcac cgtagtcgta
    68581 gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt cagaatgttt
    68641 atcaatttat cgacctgcag cgcgttgatc cgccaaagaa accgctgaag atccgtaaaa
    68701 ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg caggctgacc
    68761 gctgtctgtc gtgcggcaac ccatactgcg agtggaaatg cccggtacac aactacatcc
    68821 caaactggct gaagctcgcc aacgaggggc gtatttttga agcggcagaa ctgtcgcatc
    68881 agaccaacac actgccggaa gtatgcggac gtgtctgccc gcaagaccgt ctgtgcgaag
    68941 gttcctgcac cctgaacgat gagttcggtg cggtgaccat cggcaacatt gagcgttata
    69001 tcaatgataa agcgttcgaa atgggctggc gtccggatat gtccggcgtg aagcaaaccg
    69061 gtaaaaaagt ggcgattatc ggtgcaggcc cggcaggtct ggcgtgtgcg gatgtcctga
    69121 cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc agaaattggc gggctgctga
    69181 ccttcggtat tccggccttc aagctggaaa aagaggtaat gacgcgtcgc cgtgaaatct
    69241 tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac gtacagctgg
    69301 acgatctgct gagtgattac gatgccgtgt tccttggcgt tgggacttat cagtcaatgc
    69361 gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg ttccttatcg
    69421 ccaacaccaa acagttaatg ggctttggtg aaacccgcga cgaaccgttc gtcagcatgg
    69481 aaggcaaacg cgtggtagta cttggcggtg gcgacactgc gatggactgc gtgcgcacgt
    69541 ctgtacgcca gggggcaaag cacgttacct gtgcctatcg tcgtgatgaa gagaacatgc
    69601 cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc aaattcaacg
    69661 tccagccact ggggattgaa gtgaacggta acggcaaagt cagcggcgta aaaatggtac
    69721 gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc gttgcaggtt
    69781 ccgaacatat cgttccggca gatgcggtga tcatggcgtt tggtttccgt ccacacaaca
    69841 tggaatggct ggcaaaacac agcgtcgagc tggattcaca aggccgcatc atcgctccgg
    69901 aaggcaacga caacgctttc cagaccagca acccgaaaat ctttgctggc ggcgatatcg
    69961 tccgtggttc cgatctggtc gttaccgcca tcgccgaagg tcgcaaagcg gcagacggca
    70021 ttatgaactg gctggaagtt taagctctgt cccagcgccg cctcctcaga aaagagtcgg
    70081 cgctgaaatt ctcctttctt tctggaaatc gcctcgcata atctgaagaa aaccaatgta
    70141 atacgctcat ctggattgtt accggtagat gcccggcgta tagctgattc cattattcaa
    70201 cgcagcgcca tataaaggga cagataatgg aatcgctctc agagagaacc tcaacaggct
    70261 accagcaaat ccacaacggc attattcatc tggtcgatag cgcccgaacg gaaacggtac
    70321 gtagcgttaa cgcgctgatg accgcgacgt actgggaaat tggccgacgg attgtcgaat
    70381 ttgaacaagg tggtgaggcc agagcagctt atggtgcaca gttgattgat cggctatcga
    70441 aggatttaag tcgacggtat aaacgtgggg tctctgcaaa aaacttacgc caaatgaggc
    70501 ttttttacct cttttttcaa catgttgaaa ttcgccagac agtgtctggc gaattaacac
    70561 cattgggaat tccgcagaca ccatctgcag aattcccatc ggcaaagatt tggcagacgc
    70621 tgtctgccaa attgagaggc tgtttgatat ttccacagct tttgtattg
//
LOCUS       DAJTPG010000006        67671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTPG010000006 DAJTPG010000000
VERSION     DAJTPG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..228
                     /locus_tag="OO230_000452"
     CDS             <1..228
                     /locus_tag="OO230_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5690335.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="OO230_000453"
                     /pseudo
     CDS             623..1262
                     /locus_tag="OO230_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="OO230_000454"
     CDS             1365..1688
                     /locus_tag="OO230_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5690336.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO230_000455"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="OO230_000455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5690337.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO230_000456"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="OO230_000456"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5690338.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO230_000457"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="OO230_000457"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5690339.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO230_000458"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="OO230_000458"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5690340.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO230_000459"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="OO230_000459"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5690341.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO230_000460"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="OO230_000460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5690342.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO230_000461"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="OO230_000461"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5690343.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO230_000462"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="OO230_000462"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5690344.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO230_000463"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="OO230_000463"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5690345.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO230_000464"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="OO230_000464"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5690346.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO230_000465"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="OO230_000465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5690347.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO230_000466"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="OO230_000466"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5690348.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO230_000467"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="OO230_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5690349.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO230_000468"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="OO230_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5690350.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /gene="alaA"
                     /locus_tag="OO230_000469"
     CDS             19267..20484
                     /gene="alaA"
                     /locus_tag="OO230_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5690351.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="OO230_000470"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="OO230_000470"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5690352.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="OO230_000471"
     CDS             complement(21226..23058)
                     /locus_tag="OO230_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5690353.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO230_000472"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="OO230_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5690354.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="OO230_000473"
     CDS             complement(23806..24300)
                     /locus_tag="OO230_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5690355.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="OO230_000474"
     CDS             complement(24383..24838)
                     /locus_tag="OO230_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5690356.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="OO230_000475"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="OO230_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5690357.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="OO230_000476"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="OO230_000476"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5690358.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="OO230_000477"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="OO230_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5690359.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO230_000478"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="OO230_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5690360.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO230_000479"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="OO230_000479"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5690361.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO230_000480"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="OO230_000480"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5690362.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="OO230_000481"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="OO230_000481"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5690363.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="OO230_000482"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="OO230_000482"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5690364.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="OO230_000483"
     CDS             33414..34307
                     /locus_tag="OO230_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5690365.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO230_000484"
     CDS             complement(34355..35245)
                     /gene="rpnB"
                     /locus_tag="OO230_000484"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5690366.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO230_000485"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="OO230_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5690367.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="OO230_000486"
     CDS             complement(36223..36939)
                     /locus_tag="OO230_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5690368.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO230_000487"
     CDS             complement(36936..37622)
                     /gene="hisQ"
                     /locus_tag="OO230_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5690369.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO230_000488"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="OO230_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5690370.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO230_000489"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="OO230_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5690371.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO230_000490"
     CDS             complement(39763..40332)
                     /gene="ubiX"
                     /locus_tag="OO230_000490"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5690372.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO230_000491"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="OO230_000491"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5690373.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO230_000492"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="OO230_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5690374.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO230_000493"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="OO230_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5690375.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO230_000494"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="OO230_000494"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5690376.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO230_000495"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="OO230_000495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5690377.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="OO230_000496"
     CDS             complement(45788..46447)
                     /locus_tag="OO230_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5690378.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO230_000497"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="OO230_000497"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5690379.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="OO230_000498"
     CDS             complement(47342..48355)
                     /locus_tag="OO230_000498"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5690380.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO230_000499"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="OO230_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5690381.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /gene="flk"
                     /locus_tag="OO230_000500"
     CDS             49656..50651
                     /gene="flk"
                     /locus_tag="OO230_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5690382.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51826)
                     /locus_tag="OO230_000501"
     CDS             complement(50648..51826)
                     /locus_tag="OO230_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5690383.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO230_000502"
     CDS             complement(52081..53322)
                     /gene="fabB"
                     /locus_tag="OO230_000502"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5690384.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53481..55487
                     /gene="mnmC"
                     /locus_tag="OO230_000503"
     CDS             53481..55487
                     /gene="mnmC"
                     /locus_tag="OO230_000503"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5690385.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55608..55886)
                     /locus_tag="OO230_000504"
     CDS             complement(55608..55886)
                     /locus_tag="OO230_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5690386.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO230_000505"
     CDS             complement(55920..56468)
                     /gene="epmC"
                     /locus_tag="OO230_000505"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5690387.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56468..57277)
                     /locus_tag="OO230_000506"
     CDS             complement(56468..57277)
                     /locus_tag="OO230_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5690388.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO230_000507"
     CDS             complement(57277..58101)
                     /gene="mepA"
                     /locus_tag="OO230_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5690389.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO230_000508"
     CDS             complement(58105..59190)
                     /gene="aroC"
                     /locus_tag="OO230_000508"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5690390.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO230_000509"
     CDS             complement(59225..60157)
                     /gene="prmB"
                     /locus_tag="OO230_000509"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5690391.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60323..60874
                     /gene="smrB"
                     /locus_tag="OO230_000510"
     CDS             60323..60874
                     /gene="smrB"
                     /locus_tag="OO230_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5690392.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60978..61868)
                     /locus_tag="OO230_000511"
     CDS             complement(60978..61868)
                     /locus_tag="OO230_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5690393.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61855..62379)
                     /locus_tag="OO230_000512"
     CDS             complement(61855..62379)
                     /locus_tag="OO230_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5690394.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62376..62846)
                     /locus_tag="OO230_000513"
     CDS             complement(62376..62846)
                     /locus_tag="OO230_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5690395.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62843..63391)
                     /locus_tag="OO230_000514"
     CDS             complement(62843..63391)
                     /locus_tag="OO230_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5690396.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63396..64118)
                     /locus_tag="OO230_000515"
                     /pseudo
     CDS             complement(63396..64118)
                     /locus_tag="OO230_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64138..64860)
                     /locus_tag="OO230_000516"
     CDS             complement(64138..64860)
                     /locus_tag="OO230_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5690397.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO230_000517"
                     /pseudo
     CDS             complement(64920..66074)
                     /gene="yfcU"
                     /locus_tag="OO230_000517"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66023..66778)
                     /locus_tag="OO230_000518"
     CDS             complement(66023..66778)
                     /locus_tag="OO230_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5690398.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66860..67423)
                     /locus_tag="OO230_000519"
     CDS             complement(66860..67423)
                     /locus_tag="OO230_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5690399.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16922 a        18283 c        16784 g        15682 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa attttccttt ttttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg gtcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaggaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagt ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc
    51541 cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat
    51601 cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga atcccgacgg
    51661 caatgccgac catggtattg ccatagccca gatcatgatg aacaaacagc gggataaccg
    51721 gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg
    51781 aaaaactggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa
    51841 tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt
    51901 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    51961 agaactggtg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    52021 agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac
    52081 ctactccggc ctactgcgaa ttaatctttt cagcttgcgc attaccagcg tggcgttggt
    52141 gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg tcgtttcggt
    52201 cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat
    52261 aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag
    52321 tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc
    52381 acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag
    52441 gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc
    52501 gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc
    52561 atagatgtaa gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc
    52621 gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg
    52681 ggtgtcgttg tatttagtag atagcgcacc catcgcgtcg aattcgcagg ccatttccca
    52741 gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc
    52801 tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac
    52861 gccatgaatt ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt
    52921 gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa
    52981 cacctggaaa cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt
    53041 ctggtaagct tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc
    53101 ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc
    53161 cagttttatg ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa
    53221 agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat
    53281 gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc
    53341 gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat
    53401 cagccattta atagagatat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc
    53461 ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta
    53521 atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg
    53581 ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg
    53641 agcatccaca tcctctgttt gtgatagcag agagcggctt cggcaccgga ttaaacttcc
    53701 tgacgctatg gaaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac
    53761 gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc
    53821 atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc
    53881 ccttgcccgg ttgccatcgt ttattgctcg atgaaagccg cgtgacgctg gatttatggt
    53941 ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg
    54001 cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt
    54061 ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg
    54121 gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg
    54181 ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca
    54241 cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta
    54301 ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt
    54361 gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt
    54421 tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc
    54481 gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt
    54541 taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg
    54601 cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa
    54661 attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta
    54721 atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga
    54781 atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac
    54841 acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc
    54901 tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag
    54961 agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac
    55021 atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg
    55081 atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag
    55141 aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc
    55201 tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg
    55261 cggagcaaaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg
    55321 ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga
    55381 tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt
    55441 tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag
    55501 tgtttgccgg atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata
    55561 agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac
    55621 gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat
    55681 tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg
    55741 gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac
    55801 ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga
    55861 atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt
    55921 cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa
    55981 ttttgtagtg ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc
    56041 atcacctgcg catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc
    56101 agattgtcgc aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac
    56161 gcctgcggct tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc
    56221 gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag
    56281 tgcgaaatct catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac
    56341 ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta
    56401 ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta
    56461 ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta
    56521 atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt
    56581 tgctcaacac caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa
    56641 agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg
    56701 acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagca
    56761 taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac
    56821 cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatctt
    56881 cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca
    56941 aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc
    57001 cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa
    57061 taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg
    57121 cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac
    57181 cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca
    57241 gcggagaaac cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag
    57301 cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg
    57361 ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa
    57421 aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc
    57481 gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag
    57541 gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc
    57601 ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc
    57661 gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt
    57721 tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc
    57781 gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag
    57841 attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata
    57901 gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc
    57961 gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg
    58021 ggttattttt tgccacggcg tcgctgccag gctagcacta ctggcaagca gagccagcag
    58081 cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt
    58141 ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat
    58201 cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc
    58261 ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc
    58321 catatgagca atgatttgct gcccgctgct gataccgccg aaaatgccgc ccgcatggtt
    58381 gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac
    58441 gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc
    58501 gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc
    58561 aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc
    58621 atctaacgcg tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga
    58681 ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat
    58741 accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt
    58801 ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta
    58861 ggtgtaatca gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg
    58921 ctggtcggta ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac
    58981 accggagagt attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc
    59041 agggcgacga cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg
    59101 aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg
    59161 aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt
    59221 ttacttaatc tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga
    59281 gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat
    59341 attgttccat aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag
    59401 caaggtaatc tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag
    59461 atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg
    59521 cgtcgacata cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga
    59581 acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt
    59641 cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg
    59701 cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg
    59761 gttgcttgct gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg
    59821 gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca
    59881 ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt
    59941 tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga
    60001 gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc
    60061 agatatttgc cgcgctgaag cggcttaccg accagcgcaa tatgtcctga atggtttgca
    60121 gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc
    60181 aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg
    60241 agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa
    60301 aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc
    60361 tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg
    60421 accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga
    60481 tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa
    60541 atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc
    60601 accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg
    60661 cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca
    60721 cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat
    60781 ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga
    60841 agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc
    60901 gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt
    60961 aggcctgata agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga
    61021 aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg
    61081 tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca
    61141 caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca
    61201 tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa
    61261 agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca
    61321 taattttcac tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta
    61381 tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat
    61441 gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca
    61501 caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca
    61561 gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg
    61621 cctttatacg caaaagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta
    61681 acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc
    61741 acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca
    61801 acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa
    61861 atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct
    61921 ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt
    61981 gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc
    62041 agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag cactatcttc
    62101 tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg
    62161 aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcactgg cggcaatggc
    62221 aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg atttactgat
    62281 taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat
    62341 caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg
    62401 gcccaggcct caaagtccgc ctctggcaga atgacaccac tcttttttac tggcacagcc
    62461 gttaacaccg gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc
    62521 cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca
    62581 aaatcgcttg gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc
    62641 gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca
    62701 ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc
    62761 ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac
    62821 agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa
    62881 attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa
    62941 ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat
    63001 cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac
    63061 cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt
    63121 tacaaacaag cgagtagtca atgggacgtt tataatcaac cctattgatc ttattaatga
    63181 atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa
    63241 gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac
    63301 aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc
    63361 cggcctcagc ctttgcggct ctgctcttca tcgacctagc aactgttacc gttgcaagtg
    63421 aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg cacgctaccc
    63481 atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga gaaaccggct
    63541 gccggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg
    63601 ttattgacgg taaattcctg accgttgcgg gttaaagtaa tcttatattg ctaggggttt
    63661 ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg cgtttgtagt
    63721 gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac gttgaagtag
    63781 aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg cacttttacc
    63841 tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa cggtgaactg
    63901 atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag tttcggatcg
    63961 ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa acgggtacga
    64021 tctggcgtta cagaagcctg ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc
    64081 gtagcaccca atttggcaga gcaaagtaaa tcagacatgg atcattcctt aatcacgtta
    64141 ttggttagct gaaacagaaa cagggggatt ggtgggtgtc acctgttgcg tctgctcctg
    64201 aatgacaggc tttatgtcat caggaacaac cggcgcgacg ttgcctttat gctggcatgg
    64261 cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg
    64321 cgcaacacca ctccagaaca cactcatgtg ttcgccaggt ttcactccag ccagataaac
    64381 attgccgtca tcatcgacaa ggcccactgt ctgctcgtta tcatttttta cttctgcgcc
    64441 aaacggagga tggctgccgt cttgtaaacg cagcaccgcc atcgcttttt gaccactgat
    64501 gacggcaaat ttgcggtagc caatcgcccc ttcagttagc gtggcttgta ccaccgactg
    64561 ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata tacgcctgat tgcggtaata
    64621 gttattgacg tcagaaacga cggctttacc aaacatattg gtataaacag cagcaccgtt
    64681 accttctacc ggaacatcgg caacgccatc ggcatcaatc aacaagcgtg tcccgcccat
    64741 attctgggta cggtgaagtg cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga
    64801 caagcccgca gaggtgtatt gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag
    64861 cgaaccatca tggctgtaat agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt
    64921 caactgatag tgagtcgcgt catcgacacg gctgaaataa ccgacctgac tgctgtccgt
    64981 cccactgcca tagtaccgtt atagctaacg gtactgttgt cgccccacgg catactgagc
    65041 gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc agtcatcgag
    65101 atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat gttgtagttt
    65161 gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac cgaaacaccc
    65221 gcatcgcgga agttctggtt ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt
    65281 accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt ctcttccgag
    65341 aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt ggaataactc
    65401 acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt atccagtttg
    65461 gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca
    65521 ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa cgaccagccg
    65581 ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac atggtgtccc
    65641 cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg agtgaggtat
    65701 ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc ttcaatgcga
    65761 atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg tccagccgga
    65821 acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc tgtggtgtgt
    65881 gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca
    65941 gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc
    66001 tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gcctggcata gtagcgactc
    66061 cactcccatt tttttgagtt tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat
    66121 gttgatagtt agtctgccag tcagcacgca tacgccacgg ccctaggtta accccaaccg
    66181 tcccgttgcc gctgatctcg ttactatcat caccgccatt ttcttcgtgc cgggtttgtg
    66241 cgttgatgct gtagtccgcg acgatcccgg agatgccgtc atcccaacgt gaaggcggat
    66301 ccctgtcggg ataagtatat tcgaggtaag cctgtggcag tgaaatgact aatgcggact
    66361 ggcttaaatc agccttaatt tccatgcctt ccagttgacc ggatttcagg catttagcat
    66421 cgtggctcca ttgcagattt ttcgccacgt cttcttttaa accaaactgc gctaccagtt
    66481 ccggtgtcag acaagcatag cttttgctcg cgtcatcttc accagcatac cagtaaatat
    66541 cgtactcttc cgccaatggc tgtttattta gttgaacctg taaattgtat tttccgggct
    66601 caacatatcc ctggctggaa aaacgcttca tatcaatttt tgtgtcgcct tttaattcca
    66661 gaaaacggga atcaaactga atgtcgtctt cagcccagac actgctataa ctccctgaca
    66721 ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac
    66781 acgtttctat ccgtttttat tcttgttcgg tcaaaataca tttaaatccc ccatagcagt
    66841 gtcgctatgg gggtatgcct tacaggtaag taatctggaa ggtggtattt gcttcaaaat
    66901 tacccagatc tggcgcatca gctgcgccga ccagccaggc gttaaagttc agtgtctgct
    66961 tcgctttgcc tttgttggta gaagtgtctt tcactatttt ctggtcaata cccatgccgc
    67021 ttttgtaaga ggtacccaga gagtcaccga tcgccaggct aacattgtta aatgccgcac
    67081 cggtatcggt attgaaaatg gtgtaataat tgccgctatt tgcagaagaa acggtaccag
    67141 taaaggtagt ggtcattgtt tcctgagtat caaatacgca atcttgcaga cgaatctgga
    67201 aacttttcgg cgtggtggtg ccgttatttt tcagtttttc ggcaccgata tcacccatat
    67261 caacttccag tttgtgatcg tccggaacga tagaacacac tgagtcttca atggtgccgt
    67321 aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc cgtagaggtg aacacgccca
    67381 tcaccattgc cgcagcaata gctgttttaa caaacttact cattatttgt ccttaaacta
    67441 taaataatta aaaatataaa cattgcaata cattgattca gtcaatagcc aatgtttata
    67501 cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa ttgtattcat
    67561 tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt ttactaaaga
    67621 gtaaatgtag atttaattaa tatattgaca gaagaaaggt tatcttaagg a
//
LOCUS       DAJTPG010000007        66846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTPG010000007 DAJTPG010000000
VERSION     DAJTPG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..930)
                     /gene="cra"
                     /locus_tag="OO230_000520"
     CDS             complement(<1..930)
                     /gene="cra"
                     /locus_tag="OO230_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5690400.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDE"
     gene            complement(1012..1098)
                     /locus_tag="OO230_000521"
     CDS             complement(1012..1098)
                     /locus_tag="OO230_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5690401.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1110..1601)
                     /gene="ilvN"
                     /locus_tag="OO230_000522"
     CDS             complement(1110..1601)
                     /gene="ilvN"
                     /locus_tag="OO230_000522"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5690402.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1604..3328)
                     /gene="ilvI"
                     /locus_tag="OO230_000523"
     CDS             complement(1604..3328)
                     /gene="ilvI"
                     /locus_tag="OO230_000523"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5690403.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3447..3491
                     /locus_tag="OO230_000524"
     CDS             3447..3491
                     /locus_tag="OO230_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690404.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3646..4590)
                     /gene="leuO"
                     /locus_tag="OO230_000525"
     CDS             complement(3646..4590)
                     /gene="leuO"
                     /locus_tag="OO230_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5690405.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5249..5335
                     /gene="leuL"
                     /locus_tag="OO230_000526"
     CDS             5249..5335
                     /gene="leuL"
                     /locus_tag="OO230_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5690406.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5428..6999
                     /gene="leuA"
                     /locus_tag="OO230_000527"
     CDS             5428..6999
                     /gene="leuA"
                     /locus_tag="OO230_000527"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5690407.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            6999..8090
                     /gene="leuB"
                     /locus_tag="OO230_000528"
     CDS             6999..8090
                     /gene="leuB"
                     /locus_tag="OO230_000528"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5690408.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8093..9493
                     /gene="leuC"
                     /locus_tag="OO230_000529"
     CDS             8093..9493
                     /gene="leuC"
                     /locus_tag="OO230_000529"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5690409.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9504..10109
                     /gene="leuD"
                     /locus_tag="OO230_000530"
     CDS             9504..10109
                     /gene="leuD"
                     /locus_tag="OO230_000530"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5690410.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10158..11336)
                     /gene="setA"
                     /locus_tag="OO230_000531"
     CDS             complement(10158..11336)
                     /gene="setA"
                     /locus_tag="OO230_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5690411.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11438..11569)
                     /gene="sgrT"
                     /locus_tag="OO230_000532"
     CDS             complement(11438..11569)
                     /gene="sgrT"
                     /locus_tag="OO230_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5690412.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11658..13313
                     /gene="sgrR"
                     /locus_tag="OO230_000533"
     CDS             11658..13313
                     /gene="sgrR"
                     /locus_tag="OO230_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5690413.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13347..13446
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13477..14460
                     /gene="thiB"
                     /locus_tag="OO230_000534"
     CDS             13477..14460
                     /gene="thiB"
                     /locus_tag="OO230_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5690414.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14436..16046
                     /gene="thiP"
                     /locus_tag="OO230_000535"
     CDS             14436..16046
                     /gene="thiP"
                     /locus_tag="OO230_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5690415.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16030..16728
                     /gene="thiQ"
                     /locus_tag="OO230_000536"
     CDS             16030..16728
                     /gene="thiQ"
                     /locus_tag="OO230_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5690416.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16842..17606)
                     /locus_tag="OO230_000537"
     CDS             complement(16842..17606)
                     /locus_tag="OO230_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5690417.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17692..18570)
                     /gene="araC"
                     /locus_tag="OO230_000538"
     CDS             complement(17692..18570)
                     /gene="araC"
                     /locus_tag="OO230_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5690418.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18909..20609
                     /gene="araB"
                     /locus_tag="OO230_000539"
     CDS             18909..20609
                     /gene="araB"
                     /locus_tag="OO230_000539"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5690419.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20620..22122
                     /gene="araA"
                     /locus_tag="OO230_000540"
     CDS             20620..22122
                     /gene="araA"
                     /locus_tag="OO230_000540"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5690420.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22322..23017
                     /gene="araD"
                     /locus_tag="OO230_000541"
     CDS             22322..23017
                     /gene="araD"
                     /locus_tag="OO230_000541"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5690421.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23092..25443
                     /gene="polB"
                     /locus_tag="OO230_000542"
     CDS             23092..25443
                     /gene="polB"
                     /locus_tag="OO230_000542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5690422.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25608..28514
                     /gene="rapA"
                     /locus_tag="OO230_000543"
     CDS             25608..28514
                     /gene="rapA"
                     /locus_tag="OO230_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5690423.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28526..29185
                     /gene="rluA"
                     /locus_tag="OO230_000544"
     CDS             28526..29185
                     /gene="rluA"
                     /locus_tag="OO230_000544"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5690424.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29302..30117)
                     /gene="djlA"
                     /locus_tag="OO230_000545"
     CDS             complement(29302..30117)
                     /gene="djlA"
                     /locus_tag="OO230_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5690425.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30372..32726
                     /gene="lptD"
                     /locus_tag="OO230_000546"
     CDS             30372..32726
                     /gene="lptD"
                     /locus_tag="OO230_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5690426.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32779..34065
                     /gene="surA"
                     /locus_tag="OO230_000547"
     CDS             32779..34065
                     /gene="surA"
                     /locus_tag="OO230_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5690427.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34065..35054
                     /gene="pdxA"
                     /locus_tag="OO230_000548"
     CDS             34065..35054
                     /gene="pdxA"
                     /locus_tag="OO230_000548"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5690428.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35051..35872
                     /gene="rsmA"
                     /locus_tag="OO230_000549"
     CDS             35051..35872
                     /gene="rsmA"
                     /locus_tag="OO230_000549"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5690429.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35875..36252
                     /gene="apaG"
                     /locus_tag="OO230_000550"
     CDS             35875..36252
                     /gene="apaG"
                     /locus_tag="OO230_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5690430.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36259..37101
                     /gene="apaH"
                     /locus_tag="OO230_000551"
     CDS             36259..37101
                     /gene="apaH"
                     /locus_tag="OO230_000551"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5690431.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37179..37658)
                     /gene="folA"
                     /locus_tag="OO230_000552"
     CDS             complement(37179..37658)
                     /gene="folA"
                     /locus_tag="OO230_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5690432.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37849..39711)
                     /gene="kefC"
                     /locus_tag="OO230_000553"
     CDS             complement(37849..39711)
                     /gene="kefC"
                     /locus_tag="OO230_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5690433.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39704..40234)
                     /gene="kefF"
                     /locus_tag="OO230_000554"
     CDS             complement(39704..40234)
                     /gene="kefF"
                     /locus_tag="OO230_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5690434.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40282..41676)
                     /locus_tag="OO230_000555"
     CDS             complement(40282..41676)
                     /locus_tag="OO230_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5690435.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41735..42022)
                     /gene="fixX"
                     /locus_tag="OO230_000556"
     CDS             complement(41735..42022)
                     /gene="fixX"
                     /locus_tag="OO230_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5690436.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42019..43305)
                     /locus_tag="OO230_000557"
     CDS             complement(42019..43305)
                     /locus_tag="OO230_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5690437.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43356..44297)
                     /locus_tag="OO230_000558"
                     /pseudo
     CDS             complement(43356..44297)
                     /locus_tag="OO230_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44312..45082)
                     /locus_tag="OO230_000559"
     CDS             complement(44312..45082)
                     /locus_tag="OO230_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5690438.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45438..46952
                     /gene="caiT"
                     /locus_tag="OO230_000560"
     CDS             45438..46952
                     /gene="caiT"
                     /locus_tag="OO230_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5690439.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            46983..48125
                     /gene="caiA"
                     /locus_tag="OO230_000561"
     CDS             46983..48125
                     /gene="caiA"
                     /locus_tag="OO230_000561"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5690440.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48254..49471
                     /gene="caiB"
                     /locus_tag="OO230_000562"
     CDS             48254..49471
                     /gene="caiB"
                     /locus_tag="OO230_000562"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5690441.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49545..51098
                     /gene="caiC"
                     /locus_tag="OO230_000563"
     CDS             49545..51098
                     /gene="caiC"
                     /locus_tag="OO230_000563"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5690442.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51207..51992
                     /gene="caiD"
                     /locus_tag="OO230_000564"
     CDS             51207..51992
                     /gene="caiD"
                     /locus_tag="OO230_000564"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5690443.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            51998..52588
                     /gene="caiE"
                     /locus_tag="OO230_000565"
     CDS             51998..52588
                     /gene="caiE"
                     /locus_tag="OO230_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5690444.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52674..53069)
                     /gene="caiF"
                     /locus_tag="OO230_000566"
     CDS             complement(52674..53069)
                     /gene="caiF"
                     /locus_tag="OO230_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5690445.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(53331..56552)
                     /gene="carB"
                     /locus_tag="OO230_000567"
     CDS             complement(53331..56552)
                     /gene="carB"
                     /locus_tag="OO230_000567"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5690446.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIIEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56570..57718)
                     /gene="carA"
                     /locus_tag="OO230_000568"
     CDS             complement(56570..57718)
                     /gene="carA"
                     /locus_tag="OO230_000568"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5690447.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58174..58995)
                     /gene="dapB"
                     /locus_tag="OO230_000569"
     CDS             complement(58174..58995)
                     /gene="dapB"
                     /locus_tag="OO230_000569"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5690448.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59162..60076)
                     /gene="rihC"
                     /locus_tag="OO230_000570"
     CDS             complement(59162..60076)
                     /gene="rihC"
                     /locus_tag="OO230_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5690449.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60142..61092)
                     /gene="ispH"
                     /locus_tag="OO230_000571"
                     /gene_synonym="lytB"
     CDS             complement(60142..61092)
                     /gene="ispH"
                     /locus_tag="OO230_000571"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5690450.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61094..61543)
                     /gene="fkpB"
                     /locus_tag="OO230_000572"
     CDS             complement(61094..61543)
                     /gene="fkpB"
                     /locus_tag="OO230_000572"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5690451.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61630..62124)
                     /gene="lspA"
                     /locus_tag="OO230_000573"
     CDS             complement(61630..62124)
                     /gene="lspA"
                     /locus_tag="OO230_000573"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5690452.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62124..64940)
                     /gene="ileS"
                     /locus_tag="OO230_000574"
     CDS             complement(62124..64940)
                     /gene="ileS"
                     /locus_tag="OO230_000574"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5690453.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(64983..65924)
                     /gene="ribF"
                     /locus_tag="OO230_000575"
     CDS             complement(64983..65924)
                     /gene="ribF"
                     /locus_tag="OO230_000575"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5690454.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(65932..66150)
                     /locus_tag="OO230_000576"
     CDS             complement(65932..66150)
                     /locus_tag="OO230_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5690455.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66253..66516
                     /gene="rpsT"
                     /locus_tag="OO230_000577"
     CDS             66253..66516
                     /gene="rpsT"
                     /locus_tag="OO230_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5690456.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16221 a        18204 c        17204 g        15217 t
ORIGIN      
        1 ttcgtccagg cttgccagga caatctccag cacacgctct gcgacatcgc ggtgacgttg
       61 agccactgcc agcaccggac actgtaagaa gtcgagcagt tcgttatcgc caaaggtggc
      121 aattgccagg tcagaaggca gtttgccgtc gcgacgcagc gtgacatcca tcactccttg
      181 caacaacgca aacgacgttg tgaacagcgc ctgcggcatc ggatgcgttt ccagccattt
      241 ttcgaataac tgggcagccg cctcccgctc atagctgttg gcatacagga aatgcacttc
      301 gcgcggatca tctttccaag cagtacggaa accttgttca cgcaggaagc tgacagaaag
      361 ctccggcagc gcgccaagat aaagcaccgt ctcggcggga aacttacgta actcttccgc
      421 cagcatttcg gcatcatcct gatcggcacc aaccacgctg gtgaaatgtt cacgatcgag
      481 ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc cagcgttgat aaaaaggatg
      541 ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc tgacgctgta aaaggtgctc
      601 aatgcagcgc atttcgttgt ctggctgatc ttctgagcag gcaatcagca gttgataacc
      661 ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg gtatagctgg tgttctccag
      721 atcggggatc acaagaccaa tagaacgtgt gcgtccagca cgaagcccag ctgccacggc
      781 gttcgggtgg taattgtgct cacgcaccac agccatgact ttttcaacgg ttttgtcgct
      841 cacacggtat tgcttcgctt tgccgttaat aacatagctt gcagtggtcc gcgacactcc
      901 cgccagccga gcgatttcat ccagtttcac aattgcccct tgcgtaaaat gtaaaaacca
      961 tagccattgt actggtatgg gttaaattca ttaacatcta agcgcagaaa attaactgcg
     1021 gcaactgctt ttattccagg ttcccactga tttcgcaaaa aaagcccaac gtgacatgtt
     1081 gggctgcaaa ttgcgcactg agatcattct caacgcatta ttttatcgcc gcgcgaaagt
     1141 ccgaccacac cagagcgagc aacttccaca attttcgcca catcgcgaat cgatgctaaa
     1201 aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg tataaagcga gggggtgaca
     1261 tcgataattt gcccacggaa tatttccgta ttacgtttca cttcgtcacg cccgtaaccg
     1321 ctggcctgaa ttttcaccag catgatttcc cgctcaacat gcgcgccctg ccccaactca
     1381 ctcacgcgca agacatcgac cagcttgtgt aattgctttt cgatctgctc aagtactttt
     1441 tcatcgccca cggtctggat ggtcatacgc gataatgtcg gatcgtcggt tggcgcaacg
     1501 gtcaggcttt caatgttgta gcctcgctgg gaaaaaaggc caatcacgcg ggataacgcg
     1561 cctgattcat tttcgagtaa gactgataat atccggcgca taatcaggtt ctctccgttt
     1621 tgcttaacca catttcatcc attccgcccc cgcgaatctg catcgggtag acgtgctcac
     1681 tgccatcgac ggtaacatca acaaacacca ggcgattatt gcgcacctgt tccagcgcct
     1741 cgctaagttt gctttccagc tcatgcggat gagagatctg gatcccgacg tgaccatagg
     1801 cttccgccag acggacgaaa tcgggtagtg attgcatata agattgtgaa tgacggccgg
     1861 aatagatcat gtcctgccac tgtttcacca tccccagata gcggttattg agattcacca
     1921 ccagtacggg caactcgtat tgcaacgcgg tagacagttc ctggatgttc atctgaatac
     1981 tgccgtcgcc agtgacgcaa accacggttt cttctggcaa cgccattttg acgcccagtg
     2041 ccgcaggtaa accaaaaccc atcgtgccga gaccgccgga attgatccag cgacgcggtt
     2101 tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg cccgacatcg gacgtcacgt
     2161 aagcgtcccc attagtcaac cgccaaagag tctcgatcac cgcctgcggt ttaatctttt
     2221 cactgtgagt gtcatatttc aggcactgaa gagcgcgcca ctgttcaatc tgctgccacc
     2281 agtcgcggat ctcatccagc ggttgatggg cggattcttg cgacaggagt tcaagcattt
     2341 gttcgaggac ctggcgagca tcccccacaa tcgggatatc cgcagtcacg gttttagaaa
     2401 tggaagtagg atcaatatcg atatgcagaa cagtggcatt tgggcagtac tttgccagat
     2461 tgttcgtcgt tcggtcatca aatcgtaccc cgacggcgaa aatcacatcc gcgttatgca
     2521 tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat gcccagtgcc tgacgatgcg
     2581 ttgccggaaa cgcccccatt cccatcaatg aggaaacaac gggcagattc aacgcctcca
     2641 ccgtttcttt caactgctga tggcagcccg ccatgattgc cccaccgcct acgtagacaa
     2701 ccggtttttt tgccgctacc agcgtttgca gagcacgctt aatttgccct ttatgtccgg
     2761 tagtagtggg attgtaagaa cgcatactga ccgactcagg gcagacatag ggtaatttgt
     2821 tcgccggatt aagaatatct ttcggtaaat caacgaccac tggtcctggg cgaccacttg
     2881 ccgccagcca gaaagccttt ttcagcacct gcggaatgtc ttccgtttgc ttaaccagaa
     2941 aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc gcactcctga aaggcatcgt
     3001 aacctatcaa cgaggtcgct acctgcccgg aaaggacaac taatggaatg gaatccatat
     3061 aagcggtggc gatgccggta atcgcattgg tcgcccctgg acccgacgtt accagcacga
     3121 cgccgacttc cccggtcgca cgcgccaggc catcggccat atgcaccgcc gcctgctcat
     3181 gacgaactaa tacatgatca ataccaccca cggtatgcaa tgcatcgtaa atatcaagga
     3241 ctgcgcctcc gggataaccg aatacttgtt taacgccctg atcgataagc gatcggacga
     3301 ccatctcggc tccagacaac atctccatgg cctgcctcac tgtttgacgg aaaaaatgtg
     3361 taaaacacat taaccgtccg atcttgctta agcaattggc agccagagac ataaacgcag
     3421 ggtaataaga ataaacagga ggaaaggtga aaagaaacga taaaatcctc cattacgaag
     3481 gattaaattg ataataaatc ggctgaatcc cacaacttac agcaatttct ctattttttt
     3541 aattaatcct aaaatagaga gtccacacat tcagaataaa attctacatt caataaaaaa
     3601 tgcagaataa accagacatt catgtctggc ctattctgca ttcagttagc gtttgcaaat
     3661 tgagactaat tgttcttcca tccactgatg gcctttatcg cgcccggcag cttcatgcca
     3721 agagagataa caggttctgc tgttttgttt taacggcagc ggtaatacct gtaattctaa
     3781 ggattcagcg aactcttcag ccagccaacg cggcgcaata gcgaccaaat gcgtttgcga
     3841 caccacgcta agtacgctca tcattgccat gccctgatac gcgatactgg cttgcttatc
     3901 taccgtgtca taccaaggtt gactaaatga cgcgaaacga tcgagcgaaa ccgccgcatg
     3961 ttgttcgtta taaacatcat gtttcagtaa cgggccttta attgttggat gatttttgct
     4021 ggctaccagc accatttcat ctttaaataa tggcacgctg gtaaattcag gacgatggaa
     4081 gtcttcataa ctaatcacaa actccgtttc ctgataacgc agctgatgtt cagtgttctg
     4141 atttaatgaa gacttgaaca taacatgtat atttggcgca atctgctcaa tgtgattata
     4201 aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca caaagatgaa atacacgttc
     4261 actgctcgcg ggttcaaaac ctgaaccagg caattcattt tgtactagtt gcaatgcctg
     4321 gcgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt tgaataccac ggccataacg
     4381 aacaaaaagc tcgtcattaa acatcacctt caggcgtgca acagcgttac tgaccgcagg
     4441 ttgcgacata cccagaacat gagcggcacg ggtaatgttt tgctcctgca tcacggcatc
     4501 gaaaacggtt aataagttga gatcgaccat gcgtagctgt ggcttgctta actccgccgt
     4561 ctctgaatga tctgtttgta cctctggcat acttaactcc actgtcacac ttaactccct
     4621 ttcccttatt ggaatgcaga gaaataatcc tgaagaatat gatttatcat gcatataaaa
     4681 taagaaaaag caaaatgagt aaaattcgga aaacataaag atacgggttc tgataaaaac
     4741 agaatccatt gccgtggaat gagtcattta ccacagcata ataatccata atgttttgtt
     4801 gcgaaaacaa tctaatcata actaccgcga atactcaatc atctacaaaa tggattaaat
     4861 gtgcttattt aataattaat ttatatattt aatgcattaa ttcttaacat taattgttca
     4921 ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac atataattca atacaaatca
     4981 tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt tcctcgttga ttagatgcaa
     5041 aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg tagttaagca gaaattaata
     5101 tcgcttactt taaccaccgt agcacaatta gctaatttta cggatgcaga actcacgctg
     5161 gcgggacgtt tttattgcgt cagggttgac atccgttttt gtatccagta actctaaaag
     5221 catatcgcat tcatctggag ctgatttaat gactcacatc gttcgcttta tcggtctact
     5281 actactaaac gcatcttctt tgcgcggtag acgagtgagc ggcatccagc attaagccag
     5341 cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt ttttatgccc gaagcgaggc
     5401 gctctaaaag agacaaggac ccaaaccatg agccagcaag tcattatttt cgataccaca
     5461 ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg tgaaagaaaa actgcaaatt
     5521 gcgctggccc ttgagcgtat gggtgttgac gtgatggaag tcggtttccc cgtctcttcg
     5581 ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg ttaaaaacag ccgcgtatgt
     5641 gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg ccgaatccct gaaagtcgcc
     5701 gaagccttcc gtattcatac ctttattgct acctccccaa tgcacatcgc caccaagctg
     5761 cgcagcaccc tagacgaggt gatcgaacgc gctatctata tggtgaaacg cgcccgtaat
     5821 tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc gtacacccat tgccgatctg
     5881 gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca ccatcaacat tccggacacc
     5941 gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca gcggcctgta tgaacgcgta
     6001 cctaacatcg acaaagccat tatttccgta catacccacg acgatttagg cctggcggtc
     6061 ggcaactcac tggcggcggt acatgccggt gcacgccagg tggaaggtgc aatgaacggg
     6121 atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca tcatggcgat taaagttcgt
     6181 aaggatattc tcaacgtcca caccgccatt aatcaccaag agatatggcg caccagccag
     6241 ttagttagcc agatttgtaa tatgccgatc ccggcaaaca aagccattgt tggcagcggc
     6301 gcattcgctc actcctccgg tatccaccag gatggcgtgc tgaaaaaccg cgaaaactac
     6361 gaaatcatga caccagaatc tattggtctg aaccaaatcc agctgaatct gacctctcgt
     6421 tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg ggtataaaga aagtgaatat
     6481 aatttagaca atttgtacga tgctttcctg aagctggcgg acaaaaaagg tcaggtgttt
     6541 gattacgatc tggaggcgct ggccttcatc ggtaagcagc aagaagagcc ggagcatttc
     6601 cgtctggatt acttcagcgt gcagtctggc tctaacgata tcgccaccgc cgccgtcaaa
     6661 ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg gtaacggtcc ggtcgatgcc
     6721 gtctatcagg caattaaccg catcactgac tataacgtcg aactggtgaa atacagcctg
     6781 accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg atattgtcac caactacaac
     6841 ggtcgccgct tccacggcgt cggcctggcc accgatattg tcgagtcctc cgccaaagcc
     6901 atggtgcacg tactgaacaa tatctggcgt gccgcagaag tcgaaaaaga gttgcaacgc
     6961 aaaactcaac acaacgaaaa caacaaggaa accgtgtgat gtcgaagaat taccatattg
     7021 ccgtattgcc gggagacggt attggtccgg aagtgatgac ccaggcgctg aaagtgctga
     7081 atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag ccattacgat gtaggcggcg
     7141 cagccattga taaccacggg caaccactgc cgcctgcgac ggttgaaggt tgtgagcaag
     7201 ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg ggaacattta ccaccagacc
     7261 agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca cttcaaatta ttcagcaacc
     7321 tgcgcccggc aaaactgtat caggggctgg aagcattctg tccgctgcgt gcagacattg
     7381 ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac cggcggcatc tatttcggtc
     7441 agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc gtttgatacc gaggtgtatc
     7501 accgttttga gatcgagcgt attgcccgca tcgcgtttga atctgctcgt aaacgtcgcc
     7561 acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc ctctatttta tggcgtgaaa
     7621 tcgtcaacga gatcgccacg gaatacccgg atgtcgaact ggcgcatatg tacatcgaca
     7681 acgccaccat gcagctgatt aaagatccat cacagtttga cgttctgctg tgctccaacc
     7741 tgtttggcga cattctgtct gacgagtgcg caatgatcac tggctcgatg ggcatgttgc
     7801 cttccgccag cctgaacgag caaggttttg gactgtatga accggcgggc ggctcggcac
     7861 cagatatcgc aggcaaaaac atcgccaacc cgattgcaca aatcctgtcg ctggcactgc
     7921 tgctgcgtta cagcctggat gccgatgatg cggcttccgc cattgaacgc gccattaacc
     7981 gtgcattaga agaaggcatt cgcaccgggg atttagcccg tggcgctgcc gccgttagta
     8041 ccgatgaaat gggcgatatc attgcccgct atgtggcaga aggggtgtaa tcatggctaa
     8101 aacgttatac gaaaaattat tcgacgctca cgtagtgtac gaagccgaaa acgaaacccc
     8161 gctgttatat atcgaccgcc acctggtgca tgaagtgacc tcaccgcagg cgttcgatgg
     8221 tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa accttcgcca ctatggatca
     8281 caacgtctct acccagacca aagacattaa tgcctgtggt gaaatggcgc gcatccagat
     8341 gcaggagctg attaaaaact gcaaagaatt tggcgtcgag ctgtatgacc tgaatcaccc
     8401 gtatcagggg atcgtccacg taatggggcc ggaacagggc gtcaccttgc cggggatgac
     8461 cattgtctgc ggcgactcgc ataccgccac ccacggcgcg tttggcgcac tggcctttgg
     8521 tatcggcact tccgaagttg aacacgtact ggcaacacaa accctgaaac agggccgtgc
     8581 gaagaccatg aaaattgaag tccagggcaa agccgcgccg ggcattaccg caaaagatat
     8641 cgtgctggca attatcggta aaactggcag cgcaggcggc accgggcatg tggtggagtt
     8701 ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt atgaccctgt gcaatatggc
     8761 gatcgaaatg ggcgcaaaag ccgctctggt tgctcctgac gaaaccacct ttaactatgt
     8821 caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac gacgccgttg cctactggaa
     8881 aaccctgcaa accgacgaag gcgcaacttt cgataccgtt gtcactctgc aagcagaaga
     8941 aatttcaccg caggtcacct ggggcaccaa tcccggccag gtgatttctg tgaacgacaa
     9001 tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt gcgtcggcag aaaaagcgct
     9061 ggcctatatg gggctgaaac cgggtattcc gctgaccgaa gtggctatcg acaaagtgtt
     9121 tatcggttcc tgtaccaact ctcgcattga agatttacgc gcggcagcgg agatcgccaa
     9181 agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt cccggctctg gcccggtaaa
     9241 agcccaggcg gaagcggaag gtctggataa aatatttatt gaagccggtt ttgaatggcg
     9301 cttgcctggc tgctcaatgt gtctggcgat gaacaacgac cgtctgaatc cgggcgaacg
     9361 ttgtgcatcc accagcaacc gtaactttga aggccgccag gggcgcggcg ggcgcacgca
     9421 tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc ggacatttcg ccgacattcg
     9481 caacattaaa taaggagcac accatggcag agaaatttat caaacacaca ggcctggtgg
     9541 ttccgctgga tgccgccaat gtcgataccg atgcaatcat cccgaaacag tttttgcaga
     9601 aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga ctggcgtttt ctcgatgaaa
     9661 aaggccaaca gccaaacccg gacttcgtgc tgaacttccc gcagtatcag ggcgcttcca
     9721 ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg tgagcacgcg ccctgggcat
     9781 tgaccgacta cggttttaaa gtggtgattg cgccgagttt tgctgacatc ttctacggca
     9841 atagctttaa caaccagctg ctgccggtga aattaagcga tgcagaagtg gacgaactgt
     9901 ttgcgctggt gaaagccaat ccggggatcc atttcgacgt ggatctggaa gcgcaagagg
     9961 tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc cttccgccgc cactgcatga
    10021 tgaatggtct ggacagcatc ggcctgaccc tgcagcacga cgacgctatt gcgtcttatg
    10081 aagcaaaaca acctgcgttt atgaattaga ccccttgccc ggtcaaatga ccgggctttc
    10141 cgctatcgtc cacgtcatca aacgtcttta acctttgcgg ttaaaaataa tgcgacaaca
    10201 gaaataatcg caattaccca gtagacagca aagtgccccc aactttgtgc aattgctccc
    10261 tgaataacac cagccagaat taccccggta gagatagtgt tggtaaataa ggtggtagct
    10321 gcccccgctc ttccaggcat taaatcctga aaccatagca tcccaatacc cgcaacaatg
    10381 ccgataaata cagcgttaaa aagttgcagc gtcatcaacg ccatacggct atgaaagaaa
    10441 atcaatccag tgtaaaacag cactcctgcc accactgcaa tgaccatcat tcgccgctta
    10501 ccataacgtt tgacatagta gccagccaga atcattgctg gtatttccag tccagctgcc
    10561 gtccccatca ggaaacccgc cagtttgtct ggcaatccta aatcgctact gatccacaat
    10621 ggcatatcaa taatgtacat agtgttgcag gtccacatta acgtcgaggc gacaaataac
    10681 atccgtacgt tactatcctg ccagtcacct tgcattgata aagcattttc cgacggcagt
    10741 tctacccgcg ccacagacag aagcataaat gcaatcaata ccagactgag tgtgaatatc
    10801 ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca acgccagcat aaaggccagc
    10861 ggtggaccga ttacccatgc cagagaaagc tgcgcacgca tcaccgagct aaacatcacc
    10921 acttctcgcg ccgagttatc cgcgtattcc cgcgccagag caaataactg tggcattgcc
    10981 gtattggcca gagatgccag aagcacacca caggtgataa gcgtcagata atgacgatta
    11041 aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc aaaatataat cagttttcgc
    11101 cgatcgccct gactgtcaga acgttttgcc aaccagaggc ttacgccgat cccagcaata
    11161 gcattcaccg tataaaagag tccgatccag aaaggttgcg cgccaacctc acgactcaga
    11221 aataagctca atgtaggagc ctgtagcgcc ccggccaccc ccatcataaa agcgaccagc
    11281 ataaatgccg cgtaaacacc gttcatacgg cgagccatcg tcattatcca gatcatacgt
    11341 tcccttttta gcgcggcgag aataaaaaaa accagcaggt ataatctgct ggcgggtgat
    11401 tttacaccaa tactcagtca cacatgatgc aggcaagtca actttcagaa ttgcggtcat
    11461 cccactgcat cagtccttca agcatggtta atcgttgtgg gacgcttaac caacgcaacc
    11521 agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa ctgacgcatg gggcacctcc
    11581 ttgcttcatc gttaggaatt attgacttaa tatagggaaa ataaaattgc tgtcttttgc
    11641 gcaggagttc cccttttatg ccatctgctc gtctgcaaca acagttcatc cgcctgtggc
    11701 aatgctgcga gggtaaatcg caggacacaa cgctcaacga actggcagcg ttattgagct
    11761 gctcgcgtcg tcatatgcgt accctgctca acaccatgca ggatcgcggc tggctgacgt
    11821 gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac attcctctat accgggctgg
    11881 cgcttcagca acagcgggcg gaagacctgc tggagcagga tcgtatcgac caactagtgc
    11941 agttggttgg cgacaaagcg actgtgcggc aaatgctggt ttctcatctg ggccgcagct
    12001 tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg tccgttgcgt aatctgctac
    12061 ctggcagcgc attgcgccgt tccgaaaccc atattgcccg gcaaatcttc agttcgctaa
    12121 cgcgcataaa tgaggaaaat ggggaactgg aagcagacat cgcccaccac tggcagcaaa
    12181 tatcaccgct tcactggcgt ttctttttgc gtccaggagt ccattttcat catggtcgtg
    12241 aactggaaat ggacgacgtg atcgcctctt taaaacgaat caatacgctg ccgctctatt
    12301 cgcatattac ggacattgtg tcgccgacgc cctggacgct ggatatccat ctcacgcagc
    12361 cggaccgctg gttgccgtta ctgctggggc aagttccggc gatgatcctg ccgtgcgaat
    12421 gggaaaccct cagtaacttt gccagccatc ctattggcac cggtccgtat gcggtgattc
    12481 gcaacagcac caatcaactg aaaattcagg cattcgatga cttcttcggt taccgggcat
    12541 taatcgacga agttaacgtc tgggttctgc cggaaattgc cgacgagcca gccggtggtc
    12601 tgatgctaaa aggtccacag ggcgagaaaa aagagattga aagccgcctg gaggaaggtt
    12661 gctactattt actgttcgac agccgcaccc atcgcggggc gaatcagcaa gtcagggact
    12721 gggtaagcta tgtgctttct ccaactaatc tggtctattt cgctgaggaa cagtaccagc
    12781 aactgtggtt cccggcttat ggactgctcc cccgttggca ccatgcccgc accataaaga
    12841 gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt ttatcaggat cacagtgagc
    12901 atcgggtgat tgccgggatc atgcagcaga ttctggcaag tcaccaggtc acgctggaaa
    12961 tcaaagagat cagctacgat cagtggcatg aaggagagat cgagagcgat atctggctta
    13021 atagcgccaa ctttacgctg ccgctggatt tttcgctgtt cgcgcacttg tgcgaggtgc
    13081 cgctgctcca acactgtatt ccgatcgact ggcaagccga cgccgcccgc tggcgcaatg
    13141 gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc cagtaaagca atggtgccgc
    13201 ttatccacca ctggctgatc attcagggac aacgcagtat gcgcggcctg cgtatgaata
    13261 ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc accggatcca tgattgctgg
    13321 tagataacaa aatcactaca ctaacgccgt tctcaacggg gtgccacgca aacgcgtgcg
    13381 ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg cgaagggatt tgaggctcct
    13441 tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt taaaaaaatg tcttcccctg
    13501 ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc tgactgttta tacctacgat
    13561 tccttcgccg ccgactgggg gcctggtcca aagatcaaaa aagcttttga agccaactgt
    13621 aactgcgaac tgaaactggt ggcgctggaa gatggcgttt cgcttctcaa ccgtctacgg
    13681 atggaaggca aaaatagtaa agccgatgtg gtgctggggc tggataacaa tctgttagac
    13741 gccgccagta aaaccggact atttgccaaa agcggtgtgg cagcggatgc cgttaacgtt
    13801 cccggcggct ggaataatga cactttcgtc ccgtttgatt acggctattt cgccttcgtc
    13861 tatgacaaga acaaactgaa aaacccgccg caaagcctga aagaattggt tgagagcgat
    13921 cagaactggc gggtgattta tcaggatccg cgtaccagca cgccggggct gggtctgctg
    13981 ttgtggatgc aaaaagtcta tggcgatgac gccccacaag cctggcaaaa actggcgaag
    14041 aaaaccgtca cggtcaccaa aggctggagc gaagcctacg gcctgttttt aaaaggtgaa
    14101 agcgatctgg tactgagtta caccacctct ccggcttatc acattctcga agagaaaaaa
    14161 gataactacg ccgccgcgaa ctttagcgaa ggtcactatc tgcaggtgga agtcgccgcc
    14221 cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat tcctccagtt tatggtttct
    14281 ccggcttttc agaatgcgat cccaaccggt aactggatgt atccggtggc aaatgtcacg
    14341 ctgcctgccg ggtttgaaca attgaccaaa ccggccacca cgctggagtt cacaccagct
    14401 gaagtggcgg cacaacgtca ggcatggatt agcgaatggc aacgcgccgt cagccgttaa
    14461 ttcccggctg gttaattcca ggtgtaagcg ccgccacgct ggtggtagcg gttgcgctcg
    14521 cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa ctgggtggca gtctggcagg
    14581 acagctacct gtggcatgtg gtgcgcttct ccttctggca ggcgtttctc tcggcgctac
    14641 tctctgtcgt acccgcgata ttcctcgccc gcgccctcta tcgcaggcgc tttccgggtc
    14701 ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct cccggtgcta gttgctgttt
    14761 tcggcattct tagcgtctat ggtcgtcagg gctggctggc atcgctctgc caatcgctcg
    14821 gtctggagtg gaccttttcg ccctacggcc tgcaaggcat tttgctggcg cacgtatttt
    14881 ttaatctgcc gatggcgagc cgcttattac tccaggcact ggaaaacatt cccggcgaac
    14941 aacgtcagct tgccgcccag cttgggatgc gcggctggca ttttttccgc ttcgtcgaat
    15001 ggccgtggct acggcgacaa atcccgccgg ttgccgcgct tatctttatg ctctgtttcg
    15061 ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca ggcaaccact atcgagctgg
    15121 ctatttatca ggcgctgagt tacgactacg atcctgcccg cgcggcgatg ctggcgctgc
    15181 tccagatggt gtgttgcctt gggctggtgc tgttgagtca gcgactaagt aaggccattg
    15241 cgcccggcac cacgctgctg caaggctggc gcgatccgga cgatcgtctg catagccgca
    15301 tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt gctgccaccg ttgctggcgg
    15361 tgatcgtcga tggggtaaat cgccagttgc cggaagtgct ggcacaaccg gtgctgtggc
    15421 aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg tgtattgtgc gtagtgctga
    15481 ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg gcagaaaatg ctggcgggtc
    15541 aggcgctgga gatgagcggc atgttgatcc tcgccatgcc ggggattgtg ctggctaccg
    15601 gcttcttttt actgctcaac aacactatcg gcctgccaca atctgctgac ggcattgtga
    15661 ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa agtactggaa aacccgatgc
    15721 gcgatatcac cgcccgctac agtatgttgt gtcagtcgct gggcattgaa ggctggtcgc
    15781 gcttaaaagt ggtcgagctg cgcgccctga aacgtccact ggcgcaggcg ctggcttttg
    15841 cctgcgtgct gtcgattggt gattttggcg tagtggcgtt gttcggtaac gatgatttcc
    15901 gtaccctgcc gttttatctc taccagcaaa ttggctccta tcgcagccag gacggcgcgg
    15961 tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt taccgtgatt gaaaaactac
    16021 cggggcgaaa tgttaaaact gactgatatc acctggcttt atcaccattt gccgatgcgt
    16081 tttagcttaa cggtagaacg cggcgagcag gtggcaatcc tcgggccaag cggcgcgggt
    16141 aaaagtaccc tgctgaattt gattgccggt tttctgacgc cagccagcgg ttcgctgact
    16201 atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc gtccggtgtc gatgctgttt
    16261 caggagaaca acctgttcag ccatctgacg gtcgcgcaga acatcgggct ggggctaaat
    16321 ccggggttga aactgaacgc ggcacagcag gagaaaatgc acgcgattgc ccgtcagatg
    16381 gggattgata atttaatggc gcggttaccg ggcgagcttt ccggcggtca gcgacagcgc
    16441 gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt tattgctcga tgaaccgttc
    16501 tctgcgctcg atccggcgtt acgtcaggag atgctgacgc tggtgagcac gagctgccag
    16561 cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg aagatgcggc gcggatcgcc
    16621 acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc agggtaagac tgaagaattg
    16681 ttgagcggta aggcgagtgc ttcggcaata ttggggatta cgggttagta tgcactgtca
    16741 gatgcggcgt gaacacctta tccgacctac ggacagtgcg ttttgtaggc ctgataagac
    16801 gcggtcagcg tcgcatgagg caacccgcac aagacaatcc cctaaacccc aaccacttta
    16861 cgcaaaatat cgatatacac cggcatcaac gggtggcgaa ttaacaccac cagcgccacc
    16921 acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac ggccgcgcga caaataatga
    16981 cttaaacgat cggttgcttt accgctgcgc cataaccgcc agcacagcca gccaccaacc
    17041 cacaaaaata ccgccgtcgc cagcagcaac catttaaact cgccgctctg cattccggct
    17101 ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga agtaaaacgg tggccacagc
    17161 aggcagccga taatattcgg cgtaatgaat tttgccaccg gcagatccag cattcccgcc
    17221 accattggca ccagcggacg cgtcgggcca acaaagcgac cgaccagaat ggtgaacatg
    17281 ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg ctttgttttt cttcagaaat
    17341 gaccagcgat gcaacggctt tttaaaacgc cagcccagcc agaaggagat ccagtcgccc
    17401 agcagacaac cgacaatccc tgccagccaa gcgtgccaga aacttaactc gccgctgcca
    17461 atcagcgctc ccagccccgc catcagcacc gtaccgggta gaatcaaacc gaccagcgcc
    17521 agcgactcca gaaaggccac caacaccacc gccatcaatg aatacacggt agattgggta
    17581 ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa atggacgaag cagggattct
    17641 gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa
    17701 cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc
    17761 cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg
    17821 accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg
    17881 ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag
    17941 acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc
    18001 caggtgatcg ctgatgtact gacaagcctc gcgtacacga ttatccatcg gtggatggag
    18061 cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat tcatcgccag
    18121 cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct
    18181 gaaatacggc tggtgcgctt catccgggcg aaagaaaccc gtattggcaa atattgacgg
    18241 ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca
    18301 ttcgcgagcc tccggatgac aaccgtagtg atgaatctct cctggcggga acagcaaaat
    18361 atcacccggt cggcagacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat
    18421 ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata
    18481 accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg
    18541 cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga
    18601 aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc
    18661 gctaacccaa ccggtaaccc cgcttattaa aagcattctg taacaaagca ggaccaaagc
    18721 catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta
    18781 tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc
    18841 tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt tttggatgga
    18901 gtgaaacgat ggcgattgca attggcctcg attttggcag tgattctgtg cgagctttgg
    18961 cggtggactg cgccagcggt gaagagatcg ccaccagcgt agagtggtat ccccgttggc
    19021 aaaaagggca attttgtgat gccccgaata accagttccg tcatcatccg cgtgactaca
    19081 ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga gcttagcgtc gaacagcgcg
    19141 cagctgtggt cgggattggc gttgacagta ccggctcgac gcccgcaccg attgatgccg
    19201 acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa cccgaacgcg atgttcgtat
    19261 tgtggaaaga ccacactgcg gttgaagaag cggaagagat tacccgtttg tgccacgcgc
    19321 cgggcaacgt tgactactcc cgctatattg gcggtattta ttccagcgaa tggttctggg
    19381 caaaaatcct gcatgtgact cgccaggaca gcgccgtggc gcaatctgcc gcatcgtgga
    19441 ttgagctgtg cgactgggtg ccagctctgc tttccggtac cacccgcccg caggatattc
    19501 gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca cgaaagctgg ggcggcttgc
    19561 cgccagccag tttctttgat gagctggacc cgatcctcaa tcgccatttg ccttccccgc
    19621 tgttcactga cacctggact gccgatattc cggtgggcac cttatgcccg gaatgggcgc
    19681 agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg cgcgtttgac tgccatatgg
    19741 gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa agttatcggt acttccacct
    19801 gcgacattct gattgccgac aaacagagcg ttggcgagcg ggcagttaaa ggtatttgcg
    19861 gtcaggttga tggcagcgtg gtgcctggat ttatcggtct ggaagcaggc caatcggcgt
    19921 ttggtgatat ctacgcctgg tttggtcgcg tactcggctg gccgctggaa cagcttgccg
    19981 cccagcatcc ggaactgaaa gcgcaaatca acgccagcca gaaacaactg cttccggcgc
    20041 tgaccgaagc atgggccaaa aatccgtctc tggatcacct gccggtggtg ctcgactggt
    20101 ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa aggggtgatt accgatctta
    20161 acctcgctac cgacactccg ctgctgttcg gcggtttgat tgctgccacc gcctttggcg
    20221 cacgcgcaat catggagtgc tttaccgatc aggggatcgc cgtcaataac gtgatggcgc
    20281 tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc ctgctgcgac gtgctgaatc
    20341 gcccgctgca aattgttgcc tctgaccagt gctgtgcgct cggtgcggcg atttttgctg
    20401 ccgtcgccgc gaaagtgcac gcagacatcc catcagccca gcaaaaaatg gccagtgcgg
    20461 tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg ctttgaacag ctttatcgcc
    20521 gctatcagca atgggcgatg agcgccgaac aacactatct tccaacttcc gccccggcac
    20581 aggctgccca ggccgttgcg actctataag gacacgataa tggcgatttt tgataattat
    20641 gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc cggaaaccct gcgtcaggtc
    20701 acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg aagcgaaact gccctacaaa
    20761 ctggtgttga aaccgctggg caccacgccg gatgaaatca ccgctatttg ccgcgacgcg
    20821 aattacgacg atcgttgcgc tggtctggtg gtgtggctgc acaccttctc cccggccaaa
    20881 atgtggatca acggcctgac catgctcaac aaaccgttgc tgcaattcca cacccagttc
    20941 aacgcggcgc tgccgtggga cagtatcgat atggacttta tgaacctgaa ccagactgca
    21001 catggcggtc gcgagttcgg cttcattggc gcgcgtatgc gtcagcaaca tgccgtcgtt
    21061 accggtcact ggcaggataa acaagcccat gagcgtatcg gctcctggat gcgtcaggcg
    21121 gtctctaaac aggatacccg tcatctgaaa gtctgccgat ttggcgataa catgcgtgaa
    21181 gtggcggtca ccgatggcga taaagttgcc gcacagatca agttcggttt ctccgtcaat
    21241 acctgggcgg ttggctatct ggtgcaggtg gtgaactcca tcagcgacgg cgatgttaac
    21301 gcgctggtcg atgagtacga aagctgctac accatgacgc ctgccacaca aatccacggc
    21361 gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc tggggatgaa gcgtttcctg
    21421 gaacaaggtg gcttccacgc gttcaccacc acctttgaag atttgcacgg tctgaaacag
    21481 cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt acggctttgc gggcgaaggc
    21541 gactggaaaa ccgccgccct gcttcgcatc atgaaggtga tgtcaaccgg tctgcagggc
    21601 ggcacctcct ttatggagga ctacacctat cacttcgaga aaggtaatga cctggtgctc
    21661 ggctcccata tgctggaagt ctgcccgtcg attgccgtag aagagaaacc gatcctcgac
    21721 gttcagcatc tcggtattgg tggtaaggac gatcctgccc gactgatctt caatacccaa
    21781 accggtccag cgattgtcgc cagcctgatt gatctcggcg atcgttaccg tctgctggtt
    21841 aactgtatcg acacggtgaa aacaccgcac tccctgccga aactgccggt ggcgaatgca
    21901 ctgtggaaag cgcaaccgga tctgccaact gcttccgaag cgtggatcct cgctggtggc
    21961 gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg atatgcgcca gttcgccgag
    22021 atgcacgaca ttgaaatcac ggtgattgat aacgacaccc gcctgccagc gtttaaagac
    22081 gcactgcgct ggaacgaagt gtattacgga tttcgtcgct aagtagccac atcaggtatg
    22141 taacgcctga tgcgacgctg acgcgtctta tctggcctac acgctgcgat tttgtaggcc
    22201 ggataagcaa agcgcatccg gcattcaacg cctgatgcga cgctggcgcg tcttatcagg
    22261 cctacgcgct gcgattttgt agaccggata agcgaagcgc atccggcacg aaggagtcaa
    22321 catgttaaaa gatctcaaac gccaggtatt agaagccaac ctggcgctgc caaaacacaa
    22381 cctggtcacg ctcacatggg gcaacgtcag cgccgttgat cgcgagcgcg gcgtctttgt
    22441 gatcaaacct tccggcgtcg attacagcgt catgaccgct gacgatatgg tcgtggttag
    22501 catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc tcctccgaca cgccaactca
    22561 ccggctgctc tatcaggcat tcccctccat tggcggcatt gtgcatacgc actcgcgcca
    22621 cgccaccatc tgggcgcagg cgggtcagtc gattccagca accggcacca cccacgccga
    22681 ctatttctac ggcaccattc cctgcacccg caaaatgacc gacgcagaaa tcaacggcga
    22741 atatgagtgg gaaaccggta acgtcatcgt agaaaccttt gaaaaacagg gtatcgatgc
    22801 agcgcaaatg cccggcgttc tggtccattc ccacggcccg tttgcatggg gcaaaaatgc
    22861 cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc gcttatatgg ggatattctg
    22921 ccgtcagtta gcgccgcagt taccggatat gcagcaaacg ctgctggata aacactatct
    22981 gcgtaagcat ggcgcgaagg catattacgg gcagtaatga ctgtataaaa ccacagccaa
    23041 tcaaacgaaa ccaggctata ctcaagcctg gttttttgat ggattttcag cgtggcgcag
    23101 gcaggtttta tcttaacccg acactggcgg gacaccccgc aagggacaga agtctccttc
    23161 tggctggcga cggacaacgg gccgttgcag gttacgcttg caccgcaaga gtccgtggcg
    23221 tttattcacg ccgatcaggt tccccgcgct cagcatattt tgcagggtga acaaggcttt
    23281 cgtctgacac cgctggcgtt aaaggatttt caccgccagc cggtgtatgg cctttactgt
    23341 cgcgcccatc gccaattgat gaattacgaa aagcgtctgc gtgaaggtgg cgttaccgtc
    23401 tacgaggccg atgtgcgccc gccagaacgc tatctgatgg agcggtttat cacctcaccg
    23461 gtgtgggtcg agggtgatat gcacaatggc actatcgtta atgcccgtct gaaaccgcat
    23521 cccgactatc gtccgccgct caagtgggtt tctatagata ttgaaaccac ccgccacggt
    23581 gagctgtact gcatcggcct ggaaggctgc gggcagcgca tcgtttatat gctggggccg
    23641 gagaatggcg acgcctcctc gcttgatttc gaactggaat acgtcgccag ccgcccgctg
    23701 ctgctggaaa aactcaacgc ctggtttgca aaccacgatc ccgatgtgat catcggctgg
    23761 aacgtggtgc agttcgatct gcgaatgctg caaaaacatg ccgagcgtta ccgtcttccg
    23821 ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg agcacggctt taaaaacggc
    23881 gtcttttttg cccaggctaa aggtcggcta attatcgacg gtatcgaggc gctgaaatcc
    23941 gcgttctgga atttctcttc attctcgctg gaaactgtcg ctcaggagct attaggcgaa
    24001 ggaaaatcta tcgataaccc gtgggatcga atggacgaaa ttgaccgccg tttcgccgaa
    24061 gataaacctg cgctggcaac ttataacctg aaagattgcg agctggtgac gcagatcttc
    24121 cacaaaactg aaatcatgcc atttttactc gaacgagcaa cggtgaacgg cctgccggtg
    24181 gatcggcacg gcggttcggt ggcagcgttt ggtcatctct attttccgcg aatgcatcgc
    24241 gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc acgccagccc tggcggctac
    24301 gtgatggatt cacggccagg gctttatgat tcagtgctgg tgctggacta taaaagcctg
    24361 tacccgtcga tcatccgcac ctttctgatt gatcccgtcg ggctggttga aggcatggcg
    24421 caacctgatc cagagcacag taccgaaggt tttctcgatg cctggttctc acgggagaaa
    24481 cattgcctgc cggagattgt gactaacatc tggcacgggc gcgataaagc caaacgtcag
    24541 ggtaataagc cgctttcgca ggcgctgaag atcattatga atgcctttta tggcgtgctc
    24601 ggcaccaccg cctgccgctt cttcgatccg cgcctggcat cgtcgatcac catgcgtggt
    24661 catcagatca tgcggcagac caaagcattg attgaagcgc agggctacga cattatctac
    24721 ggagataccg actcgacttt tgtctggcta aaaggtgcac attcggaaga agaggcggcg
    24781 aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt gggcggaaac gctgcaaaaa
    24841 caacggctga ccagcgcatt agaactggag tatgaaactc atttctgccg ttttcttatg
    24901 ccaaccattc gcggagctga taccggcagt aaaaagcgct acgccgggct gattcaggag
    24961 ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg tgcgcaccga ctggacgccg
    25021 ctggcccagc agtttcagca ggagctgtac ctgcgtatct tccgcaacga gccatatcag
    25081 gagtacgtcc gccaaaccat cgacaaactg atggcgggtg aactggatgc gcggctggtt
    25141 taccgtaaac gccttcgccg tccgctgagc gaatatcagc gtaatgttcc gcctcatgta
    25201 cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg gtcgcccctt gcaatatcag
    25261 aatcgcggca ccattaagta cgtatggacc accaacggcc cggagccgct ggactaccaa
    25321 cgttcaccac tggattacga acactatctg acccgccagc tacaacccgt ggcggaggga
    25381 atactccctt ttattgagga taattttgct acacttatga ccgggcaact tgggctattt
    25441 tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg tgacgaatgc tttgccatcc
    25501 agtaccatag cgccctttcc attcctggac ctgaataaca ccactacctc ataagcacgg
    25561 tagcgggtgg ttattgcctg caattaaaga tatagagccg aacacatatg ccttttacac
    25621 ttggtcaacg ctggatcagc gatacagaaa gcgaattggg acttggaacc gttgtcgcgg
    25681 tggatgcgcg aactgtcact ttacttttcc catctactgg tgaaaaccgt ctgtacgcac
    25741 gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg tgataccatt accagccatg
    25801 acggctggca gatgcaagtc gaagaagtaa aagaagaaaa tggcttgctg acctatatcg
    25861 gtactcgcct ggatactgaa gagtccggcg tagccctgcg tgaagttttc cttgatagca
    25921 aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg gcagattgac cgtatggacc
    25981 gctttgcgct gcgttatcgc gcgcgtaagt attccagcga acagttccgt atgccgtaca
    26041 gcggcctgcg cggtcagcgt accagcctga tcccgcatca gctcaacatc gctcatgatg
    26101 ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga agtgggttta gggaaaacca
    26161 ttgaagccgg gatgatcctg catcagcaac tgctctctgg cgctgctgaa cgtgtgctga
    26221 ttatcgtccc ggaaacctta cagcatcagt ggctggtaga aatgctgcgc cgtttcaacc
    26281 tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc tcagcacgat gcttacaacc
    26341 cgtttgacac cgaacagctg gtgatttgct cgctggattt tgcccgtcgt agcaaacagc
    26401 gcttggaaca tctctgtgaa gccgaatggg acctgctggt ggtcgatgaa gcgcatcacc
    26461 tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc cattgaacaa ctggcagagc
    26521 acgtgccggg cgttctgctg ctgaccgcga ccccggaaca gctggggatg gaaagccact
    26581 tcgcccgtct gcgtctgctg gacccgaacc gtttccacga ttttgcgcag ttcgttgaag
    26641 agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat gctgctggca ggtaacaaac
    26701 tgagcaatga cgaactgaac atgctcggcg agatgatcgg cgagcaggat atcgagccgc
    26761 tgttgcaggc agcaaacagc gacagcgaag atgcccagag cgcccgtcag gagctggttt
    26821 cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt ccgtaacacg cgtaacggtg
    26881 tgaaaggatt cccgaaacgc gagctgcaca ccattaagct gccgctaccg acgcagtatc
    26941 agacggctat taaagtctcc ggcattatgg gcgcacgtaa aagtgcggaa gatcgtgctc
    27001 gcgatatgct ctacccggag cgtatttatc aggaatttga aggtgataac gccacctggt
    27061 ggaacttcga tccgcgcgtt gagtggctga tgggctacct gaccagccat cgctctcaga
    27121 aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca actggagcag gtactgcgcg
    27181 aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat gtcgattatc gaacgtgacc
    27241 gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca ggtactgctg tgctcagaaa
    27301 tcggttctga aggacgtaac tttcagttcg ccagtcacat ggtgatgttt gacctgccat
    27361 tcaacccgga tctactggag cagcgtattg gtcgtctgga tcgtatcggc caggcgcacg
    27421 atattcagat ccatgtgcct tatctggaga aaaccgctca gtcggtgctg gtgcgctggt
    27481 atcacgaagg tctggatgca tttgagcaca cctgcccgac cggacgcact atttacgata
    27541 gcgtatacaa cgatctgatt aactatctgg cttcaccgga tcaaaccgaa ggctttgacg
    27601 atctgatcaa aagctgccgc gagcaacatg aagcgctgaa agcacagctg gaacagggtc
    27661 gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa agcccaggca ctggcagaaa
    27721 gcattgaaga gcaggatgac gataccaacc tgatcgcctt cgccatgaac ctgttcgata
    27781 ttatcggtat caatcaggac gatcgcggcg acaacatgat cgtgctgacg ccgtccgatc
    27841 atatgctggt gccggacttc cctggcctgt cggaagatgg catcaccatc acctttgatc
    27901 gtgaagtggc gctggcgcgt gaagatgcac agtttattac ctgggagcat ccgctgatcc
    27961 gcaacggtct ggatctgatc ctttctggcg ataccggtag cagcacgatt tcactgttaa
    28021 aaaacaaagc gttgccggta ggtacgctgt tggtggaact gatttatgtg gttgaagccc
    28081 aggctccgaa gcagttgcag ctcaaccgct tcctgccacc gacgccggta cgtatgctgc
    28141 tggataaaaa cggcaacaac ctggcggcgc aggtagagtt tgaaaccttt aaccgccagc
    28201 ttaacgcggt taaccgtcac accggcagca aactggttaa cgccgtgcag caggatgttc
    28261 acgctatcct tcaactgggt gaagcgcaga tcgagaaatc tgcccgtgca ttgattgatg
    28321 cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct gtctcgtctg gaagctctgc
    28381 gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc cattgagagc aaccgtcagc
    28441 aggtaatgga aagcctggat caggcaggtt ggcgtctgga tgccctgcgt ttgatcgttg
    28501 taacgcatca gtaacggagc cgaaaatggg gatggaaaac tacaatccac cgcaggaacc
    28561 ctggttggtt atcctgtatc aggatgacca tattatggtg gtcaacaagc cgagcggttt
    28621 gttgtcagtg ccgggtcgtc tggaagagca caaagacagc gtgatgacgc gcattcagcg
    28681 tgattatccg caggcagaat cggtgcatcg tctggatatg gctaccagcg gcgtgattgt
    28741 agtggcgctg accaaagccg cggagcggga gttaaaacgc cagttccgcg agcgcgagcc
    28801 gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc cccgcagaag gtctggtgga
    28861 tctgccgctg atttgcgact ggccaaaccg cccgaaacag aaagtctgtt acgaaacggg
    28921 taaacctgcg cagacggaat atgaagtggt ggagtatgcg gcggataaca cggcaagagt
    28981 ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt gtgcatatgc tggcgctggg
    29041 gcatccgatt ctcggcgatc gtttttatgc atcaccagaa gcgagagcga tggcaccgcg
    29101 tttgttgcta catgcagaga tgttgacgat tacccatccg gcgtatggca atagtatgac
    29161 gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc agttcggtag gcggataagg
    29221 cgttcacgcc gcatccggca attgtgcgcc gattgcctga tgcgacgcgg gccgcgtctt
    29281 atcaggccta cagtttaaaa cttatttaaa ccctttctgc tgctttatca gctcatatgc
    29341 ctgctgaatt tcctgcgctt tctgcttcgc catctccatc atctcaggcg gcaaaccttt
    29401 cgccaccagc ttatcgggat ggtgttcact catcagctta cggtaggcac gtttgatggt
    29461 ggtcgcatca tccgtcggct tcacgcccag cacattacag gcatcttcca gcgtcgggcc
    29521 acgctgcgct tgctgccagt taccaccgcc agattgctgc tgataaccgc cgccaaactg
    29581 tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg gcgcgggaga tccctaattc
    29641 ttcagcaatg acatacagca ccgcccgttc attcgggtgc agtgaaccat cagcaaacgc
    29701 cgcctgaatc tggatctcca gaaacatacg aattaagtca aaacgaccaa agcagacact
    29761 gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct gattttccca cccggaacgc
    29821 gttttgcgcc gcagtacggg aagcgccatg aaggttcatt cggtccatca actggctggc
    29881 gatatgaata tcagcctccg tgacgcgacc tttggatttg gttaaatgcc ccatcacttc
    29941 aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg ttggcgaacc acgccatttt
    30001 acggctacgg gctttatcaa acatatggcc aattaacagg cctaacacta cgccccaaaa
    30061 gccgccgccc atcagtaagg ccacggccac gccaatgatt tttccccaat actgcatata
    30121 ttccccaaat cgacacacgg atatcagggc tatctcccac aatataaagg tgcttttacc
    30181 gttttccggc tgcggtcaat tgtgggacat cgcctataat ttgcattatc atacctgtca
    30241 ttcaatgccg tgcctaacac cactgacgct attcggacag gattaacact agcgtagaga
    30301 tgacgagtac gttagtctct gaccgtttgt cacgcgcaac gttaccgatg atggaacaat
    30361 aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc caccatgatt gccaccgccc
    30421 tttatagtca acagggactg gcagccgacc tcgcctcaca gtgcatgttg ggcgtgccaa
    30481 gctatgaccg tcctctggta cagggcgata ccaatgactt acccgtgact atcaatgctg
    30541 accacgcgaa aggggactac ccggatgacg ccgtgtttac tggcagcgtg gatatcatgc
    30601 agggtaacag ccgtctgcag gccgacgaag tgcagctcca tcaaaaagag gcaccaggac
    30661 aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt ccattacgac gataaccagg
    30721 tgatcctcaa agggccgaaa ggctgggcga atctgaacac caaagatacc aacgtctggg
    30781 aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa agcggacctg atgaaacaac
    30841 gtggcgaaaa ccgctatacc attctggata acggtagctt tacctcctgt ctgccgggtt
    30901 ctgacacctg gagcgtggta ggtagcgaaa ttattcatga ccgcgaagaa caagttgctg
    30961 agatctggaa cgcccgcttt aaggtgggtc cggtaccgat cttttatagc ccctatttgc
    31021 agttgccggt gggtgacaaa cgtcgctctg gtttcttgat cccgaacgcc aagtacacca
    31081 ccaccaacta ctttgagttc tacctgccat attactggaa catcgcgcca aatatggatg
    31141 ccaccatcac gccgcattat atgcatcgtc gtggcaacat catgtgggag aacgaattcc
    31201 gctacctctc ccaggcgggc gctggcttga tggaactgga ctatctgcct tcagataaag
    31261 tctatgaaga tgaacacccg aacgatgaca gttcacgtcg ttggttattc tactggaacc
    31321 actccggggt catggatcag gtgtggcgtt tcaacgtcga ctacaccaag gtcagcgatc
    31381 ctagctactt caatgatttc gataacaagt acggttccag tactgacggc tacgcaacgc
    31441 aaaaattcag cgttggctat gcggtgcaaa acttcaatgc caccgtttca accaagcagt
    31501 tccaggtttt cagcgaacag aacaccagta gctactcggc agagccgcag ttagacgtta
    31561 attactacca gaatgatgtt ggtccgtttg atacgcgtat ttacggccag gcagtgcact
    31621 ttgttaacac cagagacgac atgcctgaag caacccgtgt tcacctggaa ccgaccatca
    31681 atttgccgct ctctaataac tggggcagca tcaataccga agcgaagttt ctggcaaccc
    31741 attatcagca aaccaatctt gactggtata actccagaaa cacgaccaag ctggacgaat
    31801 ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa aatggtcttt gaacgcgata
    31861 tggaaatgct ggctccgggt tatacccaaa cgctggaacc gcgcgcgcag tatttgtacg
    31921 tgccgtatcg cgatcagagc gacatctata actacgactc gtctctgctg caatctgact
    31981 actctggcct gttccgggac cggacttacg gcggtcttga ccgtattgcc tccgctaacc
    32041 aagtgacgac cggtgtcaca tctcgcatat atgatgatgc tgccgttgaa cgttttaata
    32101 tttccgttgg tcaaatctac tatttcacgg agtctcgcac tggcgatgac aacataacat
    32161 gggagaatga cgacaaaacg ggctcactgg tgtgggcagg cgacacctac tggcgtatct
    32221 ccgagcgttg gggattgcgt ggcgggattc agtacgatac acgtctggat aacgtagcga
    32281 ccagtaactc cagcattgaa taccgtcggg atgaagaccg tctggtacag ctgaattacc
    32341 attacgccag cccggaatat attcaggcta cgctgcctaa gtactattcc actgctgagc
    32401 aatataagaa tggtatttcg caggtaggtg ctgtcgccag caggccaatt gccgatcgtt
    32461 ggtccattgt tggggcctac tactacgaca ccaatgcgaa caagcaagcc gactctatgt
    32521 taggtgtgca atacagctcc tgctgctatg caattcgcgt cggttacgag cggaagctga
    32581 acggttggga taacgataaa caacatgcgg tatatgacaa cgcaatcggc tttaacatcg
    32641 aacttcgcgg cctgagctcc aactacggtc tgggtacgca agagatgctg cgttcgaaca
    32701 ttctgccgta tcaaaacact ttgtgatttg ttgatttacc acgtaatccg cagtgcggtt
    32761 aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc ttctcggtat cgccatgatc
    32821 gcgaatacca gtttcgctgc cccccaggta gtcgataaag tcgcagccgt cgtcaataac
    32881 ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt cggtaaaact gaacgctgcg
    32941 caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc accaaatcat ggaacgtttg
    33001 atcatggatc aaatcatcct gcagatgggg cagaaaatgg gagtgaaaat ctccgatgag
    33061 cagctggatc aggcgattgc taacatcgcg aaacagaaca acatgacgct ggatcagatg
    33121 cgcagccgtc tggcttacga tggtctgaac tacaacactt atcgtaacca gatccgcaaa
    33181 gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc gtcgcatcac catcctgcca
    33241 caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa acgacgccag cactgagctg
    33301 aacctgagcc acatcctgat cccgctgccg gaaaacccga cctctgatca ggtgaacgaa
    33361 gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta acggcgctga tttcggtaag
    33421 ctggcgattg ctcattctgc cgaccagcag gcgctgaacg gcggccagat ggggtggggc
    33481 cgtattcagg agttgccggg gatcttcgcc caggcattaa gcaccgcgaa gaaaggcgac
    33541 attgttggcc cgattcgttc cggcgttggc ttccatattc tgaaagttaa cgacctgcgc
    33601 ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc gccatattct gctgaaaccg
    33661 tcgccgatca tgactgacga acaggcccgc gtgaaactgg agcaaattgc tgctgatatc
    33721 aagagtggta aaacgacttt tgctgccgca gcgaaagagt tctcgcagga tccaggttct
    33781 gctaaccagg gcggtgatct cggctgggct acaccagata ttttcgatcc agccttccgt
    33841 gacgccctga cccgcctgaa caaaggtcaa atgagtgcac cggttcactc ttcattcggc
    33901 tggcatttaa tcgaactgct ggatacccgt aatgtcgata aaaccgacgc tgcgcagaaa
    33961 gatcgtgcat accgcatgct gatgaaccgt aagttctcgg aagaagcagc aagctggatg
    34021 caggaacaac gtgccagcgc ctacgttaaa atcctgagca actaatggtt aaaacccaac
    34081 gtgttgtgat cactcccggc gagcccgccg ggattggccc ggacttagtt gtccagcttg
    34141 cacagcgtga gtggccggtc gaactggttg tttgtgccga tgccactctc cttaccgacc
    34201 gggcagcgat gctcggtttg ccgctcaccc tccgccctta ttcccccaac tcccctgcac
    34261 aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc gctacgtgaa tctgtcactg
    34321 cggggcagtt agcgattgaa aatgggcatt acgtggtaga gacgctggcg cgagcgtgtg
    34381 atggctgtct gaacggtgaa tttgctgcgc tgatcacagg ccccgtgcat aaaggcgtca
    34441 ttaacgacgc aagcattccg tttaccggtc ataccgagtt tttcgaagag cgttcgcagg
    34501 cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg cgtggcgctg gcaacgacgc
    34561 atttaccgct gcgcgatatc gcagatgcta tcacccctgc acttttgcac gaagtgattg
    34621 ctattttgca tcacgatttg cgtacaaaat ttggtattgc cgaaccgcgc attctggtct
    34681 gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg tacggaagag atagacacca
    34741 ttattccggt gctcgacgag ctgcgggcgc aggggatgaa actcaacggg ccgctgcctg
    34801 ccgataccct gtttcagccg aaatatctcg ataacgccga cgccgtgctg gcgatgtacc
    34861 acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg gcgcggtgtg aacattacgc
    34921 tgggcctgcc ctttattcgc acatcagtgg accacggcac cgcgcttgaa ctggcgggac
    34981 gtggcgaagc cgatgtcggc agttttatta cggcgcttaa tctcgccatc aaaatgattg
    35041 ttaacaccca atgaataatc gagtccacca gggccactta gcccgtaaac gcttcgggca
    35101 aaactttctc aacgatcagt tcgtgatcga cagcattgtt tctgccatta acccgcaaaa
    35161 gggccaggca atggtcgaaa tcggccccgg tctggcggca ttgaccgagc cggtcggcga
    35221 acgtctggac cagctgacgg tcatcgaact tgaccgcgat ctggcggcac gtctgcaaac
    35281 gcatccattc ttaggcccga aactgacgat ttatcagcag gatgcgatga cctttaactt
    35341 tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt ttcggcaacc tgccttataa
    35401 catctccacg ccgttgatgt tccatctgtt tagctatact gatgccattg ccgacatgca
    35461 ctttatgttg caaaaagagg tggtgaatcg tctggttgca ggaccgaaca gcaaagcgta
    35521 tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg atcccggtac tggaagttcc
    35581 gccgtcagcc tttacaccac cacccaaagt ggattccgcc gtcgtgcgcc tggtccctca
    35641 tgcaacgatg cctcacccgg ttaaagatat tcgcgtgttg agccgcatca ccaccgaagc
    35701 ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc aacctgttta gcgtcgaggt
    35761 gttaaccgga atggggatcg acccggcgat gcgagcggaa aatatctctg tcgcgcaata
    35821 ttgccagatg gcgaactatc tggcggagaa cgcgcctttg caggagagtt aacgatgatc
    35881 aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca ttgaggcaca atcttcacct
    35941 gataatgaac gttacgtttt tgcttatacc gtaaccatac gcaatctggg gcgagcgcca
    36001 gtgcagttgt tggggcgtta ctggctgatc accaatggca atggccgtga aaccgaagtc
    36061 cagggcgaag gagtggttgg cgtccagcca cttatcgcgc ctggcgaaga gtaccagtac
    36121 accagcggtg caatcattga aaccccgctg ggcaccatgc agggtcacta cgaaatgatc
    36181 gatgaaaatg gcgtcccttt cagcatcgac attcccgtat tccgactcgc cgttcccaca
    36241 ctcattcatt aaaagaatat ggcgacatac cttattggcg acgttcatgg ttgttacgat
    36301 gaactgatcg cattgctgca taaagtagaa tttacccccg ggaaagatac cctctggctg
    36361 acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg ttctgcgcta tgtgaaatcc
    36421 ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc tgcatctgct ggcggtattt
    36481 gccgggatca gccgcaataa accgaaagat cgcctgacac cgctgctgga agcgccggat
    36541 gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc tgcaaatcga cgaagagaaa
    36601 aagctggtga tggcccacgc agggatcacg ccgcagtggg atctgcagac cgccaaagag
    36661 tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct atcccttctt tcttgacgct
    36721 atgtacggcg atatgccaaa taactggtca ccggaattgc gggggctggg aagactgcgt
    36781 tttatcacca acgcctttac ccgtatgcgt ttttgcttcc cgaacggtca actggatatg
    36841 tacagcaaag aatcgccgga agaagcccct gccccactga aaccgtggtt tgcgattcct
    36901 ggccctgtcg ctgaagaata cagcatcgcc tttggtcact gggcatcgct ggaaggcaaa
    36961 ggtacgccgg aaggtattta cgcgctggat accggctgct gctggggtgg gtcattaacc
    37021 tgcctgcgct gggaagataa acagtatttt gtccagccgt cgaaccggca taaggatttg
    37081 ggcgaagcgg cggcgtctta aacacagcct gatacaggaa ggccggataa gacgcgaccg
    37141 gcgtcgcatc cggcactagc cgtaaattct atacaaactt accgccgctc cagaatctca
    37201 aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga attcgctgaa taccgattcc
    37261 cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt ccacttctgc gtcgatatgc
    37321 gtcagataca gtttttgcgc ttttggcagg aactgctcat aaacgcgacc gccgccaatc
    37381 accatgattt ctggtacgtc accacacgcc gcaatggctt catccaccga tttcacccac
    37441 gttacgcgat cgtccgtacc cggttgactg ctgaggataa tatttttgcg tcctggcaac
    37501 ggacgaccga ttgattccca agtatggcgg cccataatca cgggtttatt taaggtgttg
    37561 cgtttaaacc aggcgagatc ggcaggcagg ttccacggca tggcgttttc catgccgata
    37621 acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg agatttcccg ataaaaaaat
    37681 tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg atgttgagga
    37741 aaattttata ttctgctggc gagtccacgc tctccccctg gactcgccgc attacaatga
    37801 aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc actctttatt aagatgaggg
    37861 tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta ccttcttctg ttccctgcca
    37921 gccatgtcgt tgaattaatg acagatgttc gcggtcctcg gtgatgatct cacttaacat
    37981 cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg tcattctcaa ccattgccat
    38041 ctcttccacc atctgaatat taaagcggcg gaacacatcg gcacgttctc gcgcttcata
    38101 cggccccaga cctaaacttt ccagcgccag acgcccggtt ttcagcgcac cttcaaaggt
    38161 ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga atgtagtgat cgacatcgcg
    38221 ggcgcgggca ataatctgca aatgcgggaa atgttctttc accatctccg tcagttgcag
    38281 gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc gctttcgccg ctccggcaga
    38341 ttccagtaaa tccatccgcg tggcatcgcc ataaaacact ttcataccaa atttacgcag
    38401 cgtttcgata tggtccggat cgtgatcgag caccaccatt ttcaccccgc tggagagcag
    38461 taaacgtccg gtaatctgtc caaaacgacc gaatccggcg ataatcacgc gcggctgttc
    38521 ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa gattgctcaa ggcgattgag
    38581 gatcaccagc agaatcggcg ttgctgccat cgacagcgcc accgccaggg tcagcgattt
    38641 cgcccactcc ggctccagca cattcgccat ctgcgccgca ccaaatacca caaaggcaaa
    38701 ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc tgtttatttg gcacctgcaa
    38761 cggtcgggca atcagccaca gcatggcgat tttgatgatg aggaaaccga gcagcaaaat
    38821 gacaatgcgc aatgggtttt caatcagcgt gccaaagtct atcgacatgc caacaccgat
    38881 gaaaaacagc cccaacagca aacctttaaa cggttcgata tcgctttcca acgcatgacg
    38941 gtattccgag cttgccagca gtacgcccgc cagaaacgcg cccatcgcca tcgacaagcc
    39001 gacctcttcc agcagcaaac caaagccaaa cacaaggaat aacgccacgg cactaaacgc
    39061 ttcccgcaag ccagagcggg caacaaaacg cagcgccgga cgcgtaacat agcgccccag
    39121 caataccacc agtaccagcg cacccgccac ttttaacgcc gagagagcaa atgcgcccat
    39181 cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc gccaccagcg ggattgccgc
    39241 gatatcctgg aacagcagca ctgcaaaggc actgcggccc atttgcgtca ccatcagatt
    39301 gcgttcattc atcgcctgca tggcaatcgc cgttgatgag agcgccagcg tcataccgat
    39361 caattccgcg acctgccagc gcagcccaag taacatacag aacagcccca gcaggccacc
    39421 gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc gcacgcagct tccacagcct
    39481 ttgtggatcg agttcgaggc cgataataaa cagcatcagc accaccccaa tctcggcaaa
    39541 gtgcagaata gattcggcat cggtcaccag tcgcagcccc cacgggccaa taatgcagcc
    39601 ggcgatcagg tagccaagta ccgatcccag accaagacgt accgcaatgg gtacaatcag
    39661 cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta tgactatcca tgatgggcct
    39721 cctgccattc cagcagacgt tgcttatagt ggcgcgcctg cccttcgagg gtttcgtcgt
    39781 cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca gttcagcccg cagtagattg
    39841 ccgtcgcctg tagcggctgc gacagcacat caaagcccgg atgcgcacca atttcaaaat
    39901 ggctttcccc gccgccggtc gtcaccgccc acagcaaatg tttgccatgc agcgccgtgc
    39961 cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc gatccaaagt ttaagaagcg
    40021 gaggaatgct gtaccactgc atcgggtgct gccagacgat cagatcggcg cgagagagcg
    40081 cctcctgctc ggcggcaata tcgatattga agtcaggata gagttgataa agagagcgaa
    40141 tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg tttattcgca tgggaatgat
    40201 gcggatacgg atgcgcataa atgatgagga tcatagttat tagcccgcgt gtttacgcct
    40261 ttgttttccc acaaagtgta gtcatatatt cacgcggctt atagtcaata atattgattc
    40321 agaaaagaga gaaatctgaa caaataatcc cattctctgc ccgcggatcg tcatattgct
    40381 ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac gctacggaaa tcaacaagcc
    40441 aaacagcgag atacccgccc ccattagcat cgtgttactg ataccgtaat tattgataaa
    40501 tatcggcagt gcccaggtcg aaacaatggt gccaatacga cttaaggaca taatcacgcc
    40561 cacggcagag gcgcggatat ccgtcgggaa gagttcatta ggatagagcc actgcaaatt
    40621 acccggcccg ccagagaaaa aggcatacac cgcaaaggcc atcactacca gccagatccc
    40681 catatccggg attagcccca aaaccgccaa cgccagcgtc atcatggcaa agctgccaat
    40741 caacaatgga cgccgtccgg cagtgtttag ccacagcatc ggcggaatac agccgagcat
    40801 aaagaacagg ctaatcacca cattccctag tgccgcgttt ttgccaaccc ccaatcccaa
    40861 cgaaccaacg atttgcgggc caaaggtgta aatggcgaac attgggatca cctggcaggt
    40921 ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca gatcgcgaaa
    40981 acgggtttgc tgcggctgct cttcatcgaa agccaccggt tcgccaaaca gttttatcat
    41041 catctcttcg cactctttta ctcgcccttt gcgtaataac cagcggggag attctggcag
    41101 ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc gcgctaccca gcatccagcg
    41161 ccaaccacct tccacatcat aaagccagta gccgaccaga tcggcgcagg tcgcgccgac
    41221 ataccacatc gcggcaataa agctgatgga aaacgcccgc tgacgggtac tggagaactc
    41281 ggtgatcatc gaagtggcga tgggataatc tgcaccgatg acaatgccga tgagtacccg
    41341 catcaccaac agttcgacgg gggatgaaac acacatcgtc gccaccgata tcacgccgat
    41401 ggcgatgata tcaatgagga acattttgcg ccgtccgact ttatcggaga tatagccaaa
    41461 cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg cccagcaagc caatccagtc
    41521 agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc acgccaatca ttaccagtac
    41581 ataaccatcc agaaacggac caccgcttcc ccacagcaaa atgcggcggt gaatagagga
    41641 gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt tattcctgtt tttttgcaat
    41701 gagagtcagt acggcgttac gtatcaaacc aacatcagcc gtaacggaac tccacaccaa
    41761 aggtgccgcg cgggtattcc cactgttcca gcgccgaccc cagccccaga atgcgacagg
    41821 tgccgcactc cagacatccg gcgtaatcga agcgcacact gccgtcatcc tgcttcttgt
    41881 acagacctgc ggggcacgct ttcaccagca gctccagcac ctgtttatca gcatcggcct
    41941 tcacaacaat gtgcggatgc tcttcatcga cattgaattt attgacgccc agtttgacgt
    42001 ccacattgac gggagaagtc ataaaacggt cactcctttc atgccatcct tgatcagatt
    42061 gatgaagccc actttcttgc cgtggcggag gattttcttg cgcatcagtt ccggcgcact
    42121 gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc agttccgggt tgccgctaaa
    42181 catgcgtggg ttatcaagga acgccggtag tttctggtac atgcgcatat cgcgcagcgg
    42241 gccactctcc agatgctgac gatattccgc gagtttttgc ttactgaaat cgtcgctttt
    42301 catcgctgaa agcacggttt ttgctgcagc ttccccggcg gcaatcgcca gatccatacc
    42361 gcgaatggta aaaccgaggt tcatacacat tccggcggca tcaccggcaa tcaatacgcc
    42421 gtcaccgacc agctccggca gcatgttgat gcctgcttcc ggcactacgt gagcggaata
    42481 ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc ggatgctgtt tgaaatcttc
    42541 cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga tgcagaccac aaaccagccc
    42601 cagcgacagg gtattttcat tggtataaag gaagccgccg cccatcaggc catcggtggg
    42661 tgatcccgca aacagacaag ccgcgccctg attaccctgc aactgaaaac ggtcttcgat
    42721 taccgacttc ggtaactcga tcagttcctt cacgccaacc gccacatccg tcggtttaac
    42781 gcgttttgcc atccccaact tttcggcaag gatggagttc accccatcag caaggagcac
    42841 cgttttcgct tcaatcacat caccatcggc ttctacaccg acgactttgc catcgcgctg
    42901 tacgaggtta tcgacgcgga tcccggtaat taactgcgcg cccgcttctt cggcctgctc
    42961 catcaaccag gcatcaaatt tactgcgcaa aacggagtaa gaacgctggg atggcgaggt
    43021 ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt tccgtcataa acgcgagttt
    43081 ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg aaacctggaa taatgtgttc
    43141 cagactgtgg gcatagagac gcccgccagt aacgttcttg gcacctgcgg aattgccacg
    43201 ctcgataact aacacttgcg ccccttcgcg ggcgagcacc agtgcggcaa ccgaaccggc
    43261 aagccctgca ccgacgatga tggcgtcaaa gatatcttcg gacataagag ctccctgtca
    43321 gcggcagagc cgtaatgcag ccctgccaga gtggatcaac gcgctaaagc tgcggtcagt
    43381 gccggaagga tcttcacggc atcgccaaca atgccgtaat ccgcgtactg gaagatcggc
    43441 gcatttttat ctttattgat ggcgaaaatg gtttgcgacg cgttagcgcc aaccatgtgc
    43501 tggatctacc cggagatccc caccgccagg tacagttcag gtttcagcat caggttggag
    43561 ataccgacat agcgttcgtg ctccatccat ttttcgtttt ctgccaccgg acgagaacag
    43621 gccaactccg cacctatcgc cttgcaaagc tgttctgcca gcgcaatgtt ctctttgctg
    43681 ccaatgccgc gaccgacgct gaccaccaga cgggctttgt cgagatcgac gctgttgctc
    43741 tggcgcgcct gggttgccgt gcgggtaatc gccacagccg gagcctgcca ctccacggtg
    43801 tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat cgaacgtgcc gctgctgatg
    43861 gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa tcgccagacc gccgtaaacc
    43921 atgtgtttca ctgttgcttt accgtcctgt acgctgacgg tgctggcatc gttagacacc
    43981 gccgctttaa ggcgataacc cagttttgcc gccagtaatt tgccgcgacg ggtgtttggc
    44041 aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt cagccatgac accggcgtaa
    44101 tcttcgatca tccgatcgtc cggtttgccg tttaatttcc agacatgatt agcgccgagc
    44161 tggattgcct gtgcgccgtc ggcatcattg aggacaaagg cattgatttg attagctaaa
    44221 gcctgcgcac cgttcatcag ttccggcaga cgagaggggg tatcgctgaa tacccagact
    44281 tgagaaaacg tgttcatagc atcccctgta attaaatgac tttgcgaaga ttttcagcaa
    44341 atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat gcgctgacgc tcgcgctgtt
    44401 tcggcgcggc aacctgttgt tcagaccagg cgtccacggc gttaaaacca atatccgccg
    44461 ccgaccatac ctggactggc tttttcgccg cgccgagaat ggctttcatc gaaggaattt
    44521 gtggggagtt gatatcagtg gaaacagcaa caaccgcagg cagcggaatg cttaaggttt
    44581 cggtttcatc ttccagttcg cgctcaacgg tgagggtatc tgccgtcagg gagataattt
    44641 tgctgacgcc gttaactgcc ggaatattga ggatttcgcc caccagcaga ccaacctgct
    44701 gggcataaag gtcggaagaa ccatcgccac agaggatcag atcaaagcct gctttctggg
    44761 cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc ctgctcgaac tggtcatcaa
    44821 tcaccacaat cagttcatcc gggccgcgcg atagcacatc tttacgccct ttggcgttgg
    44881 tcagggcttt accgcccaca cttaaggctg tcacctgcgc ctctgctgcc tgttgcttta
    44941 gctggcaagc cgcttcaata gcgttgagat cgtattggct tattttggca tcggctttgc
    45001 tgaagtctaa tgaaccatca gcattattga ccgcaatatc ctgttcatca ggcacgcact
    45061 tatagcaagt aataatcttc attgcatctc cagaaatcat gaaggaaacg tcgttcatat
    45121 cggtggtgtt aattacagat atcacgccct ttaatttgga aataaaactt taatcaccaa
    45181 tattgaaagt cagatgctat ttttttaccc acaaaagaat ataacaaact gattaataac
    45241 caaaattaac gaaaaacgcc ctctgttcac tcgaaacaaa aatgtgatac caatcacaga
    45301 atacagctta ttgaataccc attatgagtt agccattaac gcgtccacga ggttaataat
    45361 aattatatta aatgttaaca aaaataaaac aaacgggaac gcaaaataaa tattcgtttt
    45421 cacagtggaa ttaactcatg aagaatgaaa agagaaaaac gggaatagaa ccgaaggttt
    45481 tctttccgcc attaataatc gtcggcatac tttgttggct tacagtcaga gatctggatg
    45541 cggcgaatgt cgttattaat gctgtattca gttacgtcac caatgtatgg ggatgggcat
    45601 ttgaatggta tatggtggtg atgcttttcg gttggttctg gctggtgttt ggcccgtatg
    45661 ccaaaaagcg tttaggtaac gaaccgccag aatttagcac cgccagttgg atctttatga
    45721 tgttcgcctc ctgtacgtct gctgccgtac tgttctgggg atcgattgag atctactact
    45781 acatctccac cccgccgttt ggcttagaac cgaactcgac aggagcgaaa gagttggggc
    45841 tggcttacag cttgttccac tggggacctc tgccgtgggc cacttacagc ttcctttcag
    45901 tcgccttcgc ttacttcttc tttgtccgca aaatggaagt gattcgcccc agctcgacac
    45961 tggtgccgct ggcaggtgaa aaacacgcca aagggttgtt cggtaccatc gtcgacaact
    46021 tctatctcgt cgccttgatc ttcgcgatgg gtaccagtct gggccttgcc acgccgctgg
    46081 tgaccgagtg tatgcaatgg ttgtttggca ttccgcatac cctgcaactg gacgctatca
    46141 tcattacctg ctggattatc ctcaacgcca tttgcgtcgc ttgcggtctg caaaaagggg
    46201 tacgtatcgc cagtgacgtg cgtagttacc tgagcttcct gatgctgggt tgggtgttca
    46261 tcgtcagcgg tgccagcttc atcatgaact acttcaccga ttcggtgggg atgttgctga
    46321 tgtatctgcc gcgcatgttg ttctataccg atcccatcgc taaaggcggc ttcccgcagg
    46381 gctggaccgt gttctactgg gcatggtggg tgatttacgc catccagatg agtatcttcc
    46441 tcgcccgcat ctcccgtggt cgtactgtgc gtgaactgtg cttcggcatg gtgatggggc
    46501 tgacagcatc cacctggatc ctgtggaccg tgctcggtag taacactctg ctgttgatgg
    46561 ataaaaacat catcaacatt ccaaatctga tcgaacagta cggtgtggcg cgcgccatca
    46621 tcgaaacctg ggccgctctg ccactcagca ccgccaccat gtggggcttc ttcatcctct
    46681 gctttattgc caccgttacg ctggttaacg cctgctctta taccctggcg atgtctactt
    46741 gccgcgaagt acgcgatggt gaagaaccac cgctgctggt gcgtatcggt tggtcaattc
    46801 tggttggcat tatcggtatt gttctgctgg cgctcggcgg cctgaaaccg attcaaaccg
    46861 ccattatcgc cggaggatgc ccgctgttct tcgtcaacat tatggtgacg ctctccttta
    46921 ttaaagacgc gaaacagaac tggaaagatt aattaacccc caaaatatca agaggttgaa
    46981 agatggattt taatttaaat gatgagcagg aactgtttgt cgccggtatc cgcgaactga
    47041 tggccagcga aaactgggag gcctattttg ccgagtgcga ccgtgacagc gtctacccgg
    47101 aacgttttgt caaagcactg gcggatatgg gtatcgacag tctgctgatc cctgaagagc
    47161 acggtggtct ggacgcgggg tttgttactc tcgccgccgt gtggatggag ctgggacgtc
    47221 tgggggcacc aacctatgta ctgtaccagc tgccgggcgg gttcaatacc ttcctgcgcg
    47281 aaggcacaca agagcagatc gacaagatta tggctttccg cggcaccggt aagcagatgt
    47341 ggaactcagc gattaccgaa ccgggcgcgg gctccgacgt gggtagcctg aaaacgactt
    47401 atacccgtag aaatggtaag atttatctta atggtagtaa gtgttttatt accagtagcg
    47461 cctacacccc gtacatcgtg gtgatggcgc gcgacggggc ttctccggac aaacctgtct
    47521 acaccgaatg gtttgttgat atgagcaaac cgggcatcaa agtgaccaaa cttgagaagc
    47581 tcggtctgcg tatggatagc tgctgtgaaa tcacctttga cgatgtggaa ctggacgaga
    47641 aagacatgtt cggtcgggaa ggtaacggct ttaaccgcgt caaagaagag ttcgaccatg
    47701 aacgtttcct ggtagccctg accaactacg gtacggcgat gtgcgccttt gaagatgcgg
    47761 cgcgctacgc caaccagcgc gtgcagtttg gcgaggctat tggtcgtttc cagttgattc
    47821 aggaaaaatt cgcccacatg gcgatcaaat taaactccat gaaaaacatg ctgtatgaag
    47881 cagcgtggaa agcagacaac ggcaccatca cctctggcga tgcagcgatg tgcaaatact
    47941 tctgcgccaa tgcggcattt gaagtggtgg atagcgcaat gcaggtgctg ggcggtgtcg
    48001 ggattgcggg caaccaccgc atcagccgct tctggcgtga cctgcgtgta gaccgcgttt
    48061 ccggaggatc tgacgaaatg cagatcctga cgctgggtcg tgcggtgctg aagcaatacc
    48121 gctaagtccg tgcgctgcct gatgcgacgc tgacgcgtct tatcaggcct acgggagcac
    48181 gaatgtaagc cggataaggc gtttacgccg catccggcag tcatgcgcta cataaatttt
    48241 tcaggagcta ataatggatc atctacccat gccgaaattc ggaccgctgg ccggattgcg
    48301 cgttgtcttc tccggtatcg aaatcgccgg accgtttgcc ggacaaatgt tcgcagaatg
    48361 gggcgcggaa gttatttgga tcgagaacgt cgcctgggcc gacaccattc gcgttcaacc
    48421 gaactacccg cagctctccc gccgcaattt gcacgcgctg tcgttaaata ttttcaaaga
    48481 tgaaggccgc gaagcgtttc tgaaattaat ggaaaccacc gatatcttca tcgaagccag
    48541 taaaggtccg gcctttgccc gtcgtggcat taccgatgaa gtactgtggc agcacaaccc
    48601 gaaactggtt atcgctcacc tgtccggttt tggtcagtac ggcaccgagg agtacaccaa
    48661 tcttccggcc tataacacca tcgcccaggc cttcagtggt tacctgattc agaacggtga
    48721 tgttgaccag ccaatgcctg ccttcccgta taccgccgat tacttttctg gcctgaccgc
    48781 caccacggcg gcgctggcag cactgcataa agtgcgtgaa accggtaaag gcgaaagtat
    48841 cgacatcgcc atgtatgaag tgatgctgcg tatgggccag tacttcatga tggattactt
    48901 caacggcggc gaaatgtgcc cgcgcatgag caaaggtaaa gatccctact acgccggttg
    48961 cggtctgtat aaatgtgccg acggctacat cgtgatggaa ctggtgggca ttacccaaat
    49021 tgaagagtgc tttaaagata ttggcctcgc acatctactc ggtacgccgg aaatcccgga
    49081 aggcactcag cttatccacc gtatcgaatg cccttacggc ccactggtag aagaaaaact
    49141 cgatgcctgg ctggcggcac ataccatcgc agaagttaaa gaacgctttg ccgaactgaa
    49201 tatcgcctgt gccaaagtac tgaccgtacc ggaactggaa agcaatccac agtatgttgc
    49261 ccgtgaatca atcactcagt ggcaaacgat ggatggtcgc acctgcaaag ggccgaacat
    49321 catgccgaaa ttcaaaaata accccggaca aatctggcgc ggaatgccct cacatggcat
    49381 ggacacggct gccattttga aaaatatcgg ctacagcgaa aacgacattc aggagttggt
    49441 cagcaaaggt ctggccaaag ttgaggacta aacgcgtggc gtcagtcggc ttgcctgact
    49501 agcgcccgcc caataacaaa cgaacaaaaa tggatagagg tgcaatggat atcattggcg
    49561 gacaacatct acgtcaaatg tgggacgatc tggcggacgt ttacggtcat aaaacggcgc
    49621 tgatttgtga atccagcggc ggagtcgtta accggtatag ttatcttgag ttaaatcagg
    49681 agattaaccg cacggcaaac ctgttttata cgctggggat tcgcaaaggc aacaaggttg
    49741 cactacatct cgacaactgc ccggaattta tcttttgctg gttcgggctg gcaaaaattg
    49801 gcgcgattat ggtgccgatt aacgcccgcc tgttacgcga ggaaagcgag tggatcttgc
    49861 aaaatagcca ggcgtgcctg ctggtgacca gtgcgcaatt ttatcctatg tatcaacaga
    49921 ttcagcagga agatgccact caattgcggc acatttgcct gacagatgtg gcacttcccg
    49981 ctgatgatgg agtgagttca tttactcaac tgaaaaatca acaacctgcc accttgtgct
    50041 atgcaccgcc gctatcgact gacgatacgg cggaaattct cttcacctcc ggcaccacct
    50101 cccgaccgaa aggtgtggtg attacccatt acaacctgcg cttcgctgga tattactccg
    50161 cctggcagtg tgcactgcgt gacgatgacg tctacatgac ggtaatgcct gcgtttcata
    50221 tcgattgcca gtgtactgcg gcgatggcgg cgttttctgc cggggccacc tttgtgctgg
    50281 tcgagaaata cagcgcccgc gccttctggg gacaggcgca gaagtaccgc gccaccatta
    50341 ccgaatgtat tccgatgatg atccgtacgt tgatggtgca gccgccttca gcgaacgatc
    50401 ggcaacaccg cctgcgggaa gtgatgtttt atctcaactt gtcggagcag gaaaaagacg
    50461 cattttgtga acgcttcggc gttcgcttgc tgacgtctta tgggatgacg gaaaccattg
    50521 tgggcattat cggcgatcgc cctagcgata aacgacgctg gccgtcgatt ggtcgggcgg
    50581 ggttttgcta cgaagcggag atccgcgacg atcacaatcg cccgctcccg gcaggtgaga
    50641 tcggtgaaat ctgtattaaa ggcgtaccag ggaaaaccat cttcaaagag tattttctca
    50701 acccgaaagc cactgcgaaa gtgctggaag ccgacggctg gctacatacc ggcgataccg
    50761 gataccgcga cgaagaaggc tttttttatt tcgtcgatcg ccgctgcaat atgatcaaac
    50821 gcggtggcga gaatgtctcc tgcgtggagc tggaaaatat catcgccacc catccgaaaa
    50881 ttcaggacat cgtggttgtg ggtattaaag attcgattcg cgatgaagcc atcaaagcat
    50941 ttgtggtgct gaatgaaggt gaaacattga gcgaagagga attttttcgc ttctgcgaac
    51001 aaaatatggc gaaatttaaa gtgccctctt atctggagat cagaaaagat ctgccacgta
    51061 attgctcggg gaaaataatt agaaagaatc tgaaataaat gaaacgtcag ggaactaccc
    51121 tgccagctaa taatcacgcc ctaaaacaat atgcattttt tgcagggatg ttgtcgtccc
    51181 taaaaaagca aaaatggaga aaaggaatga gtgaatcatt acatctgacc cgcaatggat
    51241 caattctgga aattaccctt gatcgcccaa aagcgaatgc tattgatgca aaaaccagct
    51301 ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc gcaattacgt gtcgccatta
    51361 ttaccggtgc cggagagaaa ttcttttccg cgggctggga tttaaaagcg gcagcagaag
    51421 gcgaagcacc ggatgctgac tttggcccgg gtggttttgc gggattaacc gaaattttca
    51481 atctcgacaa accagttatc gcagctgtga acggctacgc ctttggcggc ggtttcgaac
    51541 tggcgctggc ggcagatttt attgtttgtg ccgataacgc cagcttcgcc ctgccggaag
    51601 ccaaactggg catcgttcct gacagcggcg gtgtgctgcg tctgccgaag atcctgccgc
    51661 ctaccatcgt caatgaaatg gtgatgaccg gcagacgaat gggcgcagaa gaggcgctgc
    51721 gttgggggat agtcaaccgc gtggttagcc aggcggaact gatggataac gcccgcgaac
    51781 tggctcagca gctggttaac agcgccccgc tggcgattgc ggcgctgaaa gagatctacc
    51841 gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta tattcgcagc ggcgtgttga
    51901 aacactatcc atcggttctg cattcggaag atgccattga agggccgctg gcgtttgccg
    51961 agaagcgcga tccggtgtgg aaaggacgtt aacaaccatg agctattacg cctttgaggg
    52021 gttaattccg gtggttcacc cgacggcgtt tgtccatccc agtgccgtct tgattggcga
    52081 tgtgattgtg ggagccggtg tctacatcgg cccactcgcc tcactgcgtg gtgactacgg
    52141 gcggttgatc gtgcaagcgg gagccaatat tcaggatggc tgcattatgc atggctactg
    52201 cgacactgac accatcgttg gggaaaacgg ccatatcggg catggagcga tcctgcatgg
    52261 ttgtgtgatt ggtcgcgatg cattggtcgg gatgaacagc gtaattatgg atggcgcggt
    52321 cattggcgaa gagagcattg ttgccgcaat gagctttgtc aaagcaggct ttagtggcga
    52381 gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta cgcagtgtta gtgacgacga
    52441 gttacactgg aaacggttga ataccaaaga gtatcaggat cttgtcgggc gctgtcatgc
    52501 atcgttacat gaaacgcagc cactgaggca aatggaggaa aatcgccccc gtttgcaggg
    52561 gacgacggat gtgacgccta aacggtgatc ttgccggatg cgacctggtt gctggataag
    52621 atgctacaac atcgtatctg gcaagcagga cgcccggtat caacgagata aaattaacga
    52681 cacatactct ttgacaacat catctgccac ttctgctcgc ggttaagttc cgtgagcgga
    52741 acggcaggaa tacgcggtgt ctttcttacg ccgactaatt tttcccgact gttattgcgc
    52801 aggcgcgcgt aaatttgctc atcgatatcg accactttaa ccagtcgctg acactggcat
    52861 ccccgccctt ccagcttatt agggatcatc ttaacttcgc agcttatttc tgtgacttcc
    52921 gacagaatat aagtcagggt attaaccgcc ttgctgtgtt caatatcgaa atgaatagag
    52981 atctctcttg ctatcaccca ccgattttca gccatcatcc attcggcgat taacaagtag
    53041 agtggttttt caacatatcc ttcacacata cgaactccag tttcacaaat taaaagtcta
    53101 gctatgactc gttttgaagc aattaaacaa ggccatatta aaattgtgga tatatcgata
    53161 gtatgcaact tcaccgtcga taagtgtgag ctaaatcctg cttatgtcat caaaaatatc
    53221 gattccccaa aagatttact taatgggcaa aaaaaaacgg tcctcatcag agaaccgtat
    53281 tagttattcg atcaaattag cggatgaaaa atatccgcca cgacacgcta ttatttgatc
    53341 tgtgcgtgca tttcctgcac cgaaattact ttttcagtcg catcggcatt cagcgccatc
    53401 gcggtagcga aaccaccgtt cagggtggtg tcataatgca ctttatattg cagcgcactg
    53461 cgacggatca cgcgggagtc ttcaatcgca cgacggcctg aggtggtgtt gatgatgtag
    53521 gtatattcgc cattcttgat acggtcctga atgtgcggac ggccttcatg caccttgttt
    53581 accagacgcg gattgatacc cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc
    53641 agctcgaagc cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct
    53701 tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc
    53761 gctttggcga acgcttcagc gaaggtgcgg cccacgccca tgacttcccc ggtagagcgc
    53821 atttctggcc ctaacagcgg gtcaacgcct gggaatttat tgaacggcag caccacttct
    53881 ttcaccgagt agtacggcgg aataacttct ttggttacgc cctgctcagc cagcgatttg
    53941 ccagccatca cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac
    54001 ggaacagtac gcgccgcacg cgggtttact tcaatcaggt aaacttcgtt gtttttcacc
    54061 gcaaactgca cgttcatcag accgcgcacc tgcaattcga aggccagttt ctgcacctgc
    54121 tggcgcatca catcctgaat ttcctgactt aaggtgtacg ctggcagcga acatgcggag
    54181 tcaccggagt gcacacccgc ctgctcgatg tgctctatga tgccgccaat cagcaccatt
    54241 tcgccgtcgc agatggcatc cacgtcaact tctactgcgt catcaaggaa atgatccagc
    54301 aacactggcg catcgttaga cacgctaacc gccgtctgga agtagcgacg caggtcagct
    54361 tcgtcataga cgatttccat cgcccgaccg ccgagaacgt aagacggacg caccaccagc
    54421 gggtagccaa tctcttttgc cttctcaact gccatttcaa tagcagtaac ggtggcgttc
    54481 gccggttgtt tcagtttcag acggtcaacc gcatgctgga agcgttcacg gtcttccgca
    54541 cggtcgatag catccgggct ggtgccgata accggtacgc cagcggcttc cagtgcacgc
    54601 gccagtttca gcggggtctg accgccgtac tgaacgataa cgcctttcgg cttctcaata
    54661 cgcacgattt ccagcacatc ttccagagtt accggctcga agtagaggcg atcggaagtg
    54721 tcgtagtcgg tagagacggt ttccgggtta cagttaacca taatggtttc gtaaccgtct
    54781 tcacgcagcg ccagcgaggc gtgtacgcag cagtagtcga attcgatacc ctgaccgata
    54841 cggtttggac cgccgccaag caccatgatt ttttcacggt cggtagacgg attcgcttcg
    54901 cactcttctt cataagtgga gtacatgtaa gcggtgtcgg tggcaaactc tgccgcacag
    54961 gtatccacgc gcttgtagac cgggtgcagg tcatattggt cacgcagctt acggatttcc
    55021 gcttcgcgta cgcccgccag ttttgccaag cgcgcatcgg caaagccttt gcgtttcagc
    55081 tggcgcagga attcagcgtt caggccagtg atgcccactt ccgccacttt ctcttccaga
    55141 cgcaccagtt cttcaatctg taccaggaac cagcggtcaa tgttagtcag gttgaagacg
    55201 ccgtccacag acaggcccgc gcggaaagca tcggcgatgt accagatacg ctctgcgcct
    55261 gcgtctttca gttcgcgacg gattttggtt aacgcttccg ggtcatccag gctcactttc
    55321 gggtcgaagc cagttgcgcc cacttccagg ccgcgcagcg ctttttgcag ggattcctgc
    55381 tgcgtgcgac caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg
    55441 ttagcaccgg cgaatttttc gaagttgaag cgaggaattt tagtaaccac atagtcgatg
    55501 gacggctcga aggaggccgg agtacgtccg ccagtgatgt cgttcatcag ttcgtctagg
    55561 gtgtaaccca ccgccagttt cgccgccact ttagcaatcg ggaaaccggt cgctttcgac
    55621 gccagcgccg aagaacggga cacgcgtggg ttcatttcga taacaatcag gcgaccgttt
    55681 ttcgggttca ccgcaaactg gacattggaa ccaccggttt caacgccgat ttcacgcagc
    55741 accgccatcg aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc
    55801 gcgacagtga tggagtcacc ggtgtggatg cccatcgcat caaagttttc gatagagcag
    55861 acgatgatgc agttgtcgtt tttatcacgc accacttcca tctcgtactc tttccagccg
    55921 atcagcgact catcaatcag caactctttg gtcggagaga gatccagacc gcgggcgcaa
    55981 atttcttcaa actcttcacg gttataagcg ataccgccgc cgctaccgcc catggtaaag
    56041 gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa cagccagcgc ttcttccatc
    56101 gtgtgtgcga taccggaacg cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa
    56161 cgacggcggt cttctgcttt atcaatcgca tcggcagtgg caccaatcat ggtgacaccg
    56221 aactcttcca acacgccctg acgttccagc tccagcgcgc agttcagcgc cgtctgaccg
    56281 cccatggttg gcagcaccgc gtccgggcgc tctttttcaa taatcttgcg taccacttcc
    56341 cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc
    56401 gggttggagt tcaccagaat gacgcggtaa ccctcttcgc gcagggcttt acatgcttgc
    56461 gcgccagagt agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc
    56521 aggatacttt ttatatctgt acgttttggc atggctcttt tactcctgat tacttagcgg
    56581 ttttacggta ctgctcaatt aacttgataa agtggtcgaa caacggcgcg gcgtcgtgtg
    56641 gaccagggct ggcttcaggg tgaccctgga agctgaacgc cggcttatcg atgcgatgaa
    56701 tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt gcaggtaatg
    56761 ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat catcaccacg tttttctcca
    56821 catctttaac cggatggttg ccgccgtggt gaccaaattt cattttgaca gtcttcgcac
    56881 cgctcgccag cgccagcagc tgatgaccga gacagatgcc gaataccgga atatcggttt
    56941 cgaggaactt ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt
    57001 tggagaggaa gatgccgtct ggattcattt tcagcacatc ttccgcagaa gtttgcgccg
    57061 gaacgatggt caggcgacag cctctatcca ccagcatccg caggatgttg cgcttggcac
    57121 caaaatcata agccacgacg tggaacggca gctcgtcttc ttttttcgct tctggcaggc
    57181 cgccggtcaa cgtccagctc ccttgtgtcc agctataggc ttctgcggtg gtcacttctt
    57241 ttgccagatc catgccgttc agacctggga acgcgcgggc tttttctaac gccagcgccg
    57301 catccgggtt atcgcctgcg ataatgcagc cattctgtgc gcctttctcg cgcagtaaac
    57361 gcgtcagctt acgggtatcg atatcggcaa tcgccacgat gttatggcgc ttcaggtaag
    57421 aagagaggtc ttcggtatta cggaagttgc tggcaatcag cggcaggtcg cgaatcacca
    57481 gaccttgtgc atgtacctga gaagattctt catcggcgtc attagtgccg acattgccaa
    57541 tatggggata agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt
    57601 gataaccggt cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta
    57661 tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa
    57721 caccctccag agaatactca ctcactttat ttgcatatta attcacagtg atgacgtaaa
    57781 tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg
    57841 cgctaaaagt caacttaatg gcaggatttt ttatattatt ttgtggctca tgcatgtttt
    57901 acaggcatta agcgacaaaa acatcatcta acctgttctg gaaaacgctt gcgcagcaag
    57961 aagattgcat gaaataaaaa cgagcaggaa aaagtggacc aaatggtcaa aatttacatt
    58021 aaaacaacaa aaatagaaga cgaaacatgt aatttttggc agttatagaa aatttaaaag
    58081 cataacacca aaaataaaag gactaaaaat taaagccctt tataattaat aaattaaatg
    58141 tgttactttt gcatcataat aaacattttg tggttacaaa ttattgagat caagcacatc
    58201 tcgcatatca aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcacc
    58261 gttagcaaat gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc
    58321 ggcaaacatc gcggtatgtt cgccaacgat gtcacctgca cgcacggtag caaaaccaat
    58381 ggtacctggc acacgttcac cggtatggcc ttcacgactg tagaccgcgc aatctttcag
    58441 atctttatca agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc
    58501 atcaacttta tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac
    58561 tttggctgct ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc
    58621 aaagacaatc gcaatatcgg cggcggcgtc acgaattgcc tgtttaccgg cttcgtcaaa
    58681 ccccgtagtg ccgatcacca tccctttgcc atgctgccga caaaaagcga gatggttcag
    58741 cgtaccttcc ggacgggtaa aatcgataaa cacatcaaaa tcatctttta tcgcatcaag
    58801 actgctttgc acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc
    58861 cagtaaagaa gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag
    58921 tgccgcctga atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat
    58981 gtttgcatca tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac
    59041 cagcgcatcg ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg
    59101 gagggatatc agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag
    59161 gttacggcac cagagccagc acctcagcca cccactgttg gaagcctttt acattcagat
    59221 ccaatgccac ctgtacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg
    59281 tgcctgaggt aaattcgcct tgagtttcta ccgcgacaaa acacggcttg agggtgaata
    59341 agtccgggcg caccagccag gcgatggcgc agagatcgtg cattcgcaga ccgctttgca
    59401 tactgccgct acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact
    59461 ccggtaaagt agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc
    59521 acatgacaat ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga
    59581 tattaaactc ggcatttggt gtacagttgc cgcgaccagc agaaccgccc atgatcacca
    59641 gacgacgaat atacggttta cattccgggc attgtaaaag taacagcgca atattggtta
    59701 acgggccgat ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg
    59761 ccagaaacgc cggtatcccg agtggctttc ggttgtgctc aacaaagtcg tagccagcca
    59821 ttcccgattc gccgtgcaca gatgctgcat cacgcggtgc gcgtaccagc ggcacagcgg
    59881 ctccctgggc gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcattgcggg
    59941 tagttttctc aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg
    60001 gggcaaaaat cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga
    60061 agataggtaa acgcatgttt tcttcataaa aaatgccggt aacaagaccg gcattttcgc
    60121 ataacttagg ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg
    60181 cacttcgaaa acaatatttt cttcacggcc ttccagcgga atggcttcac caccacccaa
    60241 ctgctgcaaa cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt
    60301 cacgccgacg catttaacct ctttcaccca ctcttcctgg atatctttcg catcgtcaat
    60361 caaaaacgcg tgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga
    60421 gtttttcgaa ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc
    60481 ctgacggtta gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa
    60541 gcgtttacgc agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtttgggt
    60601 cataaaggag agcttctctt cgtttttgac cgtcagtttc cacacatcgt ctggcgattc
    60661 gactagatac attccccctt cggggttgct gtactgcccc atcgtccctt ccacttccgg
    60721 gtgcccggcg tggccgatga gaatagattc ttcgccacgg cgactggcgc gggcaacttc
    60781 catatgcact ttggtcacca gcggacaggt ggcatcaaac accgtcaaat cgcggctttt
    60841 cgcttcgtta cgaaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc
    60901 cggcacttcg ctaatctgct caataaagat agccccacgc tcgcgcaagc tatcgaccac
    60961 gtagcggtta tgcaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc
    61021 gttttcaaca atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa
    61081 caggatctgc atgttacgcc tccagtgccg gatcgatttc cagcacttca atatcaaaat
    61141 gaacggtctg cccggccagc ggatggttga aatcaacggt aatggagtcg ccgttaattt
    61201 cgcggatcac accaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa
    61261 tttctggctc gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg
    61321 acggcacgcc aaacgccgca tctggctcca gcgagaaggt ggttttgtcg cccactttca
    61381 gccccagcag gtgttgctcc agcccttcag aaagagaagc atcgcccagg cggaacagcg
    61441 ccggtttacc gttgttgcga gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga
    61501 agtgcaccag gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttatt
    61561 tttgaggatt tttgccggat gcggcctacg atttcgtagc aatctgtagg ccgcatccgg
    61621 caggatttat tattgttttt tcgctttgga aggtaaaaac ccttccagca cgatcagtgc
    61681 cgcaccgaca caaatggcag tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac
    61741 gtagaagtcg atcatatcga caacgaagcc gtgccacagg cggtcgaaca ggttgcccag
    61801 cgcgccgcca ataatcagcg cgtaagcgat attgtttagc ttctgcgtgg ccttcgagcg
    61861 atacatcatc actgccagga gcacgctaat accaatcgca ataccggcaa agaaccaacg
    61921 ctgccagccg ccgctatcgg caaggaaact aaacgccgcg ccatagttac gcgcataatg
    61981 cagattaagc gacgggaaca gcgggaccgt atcccccaga gcaaagttct ggaggatcag
    62041 gtatttgctg cccagatcga taatcagcac gactaccacc agccacagcc agcgtagccc
    62101 tgttgaacag atcgattgac tcatcaggca aacttacgtt tttcaccgtc accggcgacg
    62161 ttgctgacac agcggccgca gatttctgcg tgttccgcca ccttgccgac atcctgggtg
    62221 tagtgccagc agcgtgggca cttctcacct tcggctttac tcaacgcgac tttcagccct
    62281 ttgagtactt cgctctgctg agcatcagca ggtgcgtcgt tatagtctgc aacggtagcg
    62341 ccggaggtca acaggacaaa tcgtaattca tcgcccagcg cggtcagttt cgccgccagt
    62401 tccggttctg catacaaggt tactgccgct tccagcgagc cacccacttt cttgtcggca
    62461 cgcgcttgct caatgacttt gttcacttcg ccacgcactt tcaacagctc gtcccagaac
    62521 gcatcgttca ttgcttcact gtctgccaga ccaaacaggc cttcgtacca ctcaccggtg
    62581 aagacgtatt tttcacgttc gcccggcaga tagccccaca cttcatcagc ggtgaaggag
    62641 aggattggtg ccatccagcg caccagcgct tcagcgatgt gatacagcgc agtctggcag
    62701 ctacgacgcg ccacgctatc cgctttggcg gtgtactgac ggtctttgat gatgtcgagg
    62761 tagaaggaac ccatctcaac ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa
    62821 tcgtatgctt cgtacgcctt gaggatgtct tcctgtgccg ctttcgcaca acctacggcc
    62881 cagcgatcca gtaccaccat ctcttccggt ttcaccatat cttttgctgg atcaaaacca
    62941 ttcaggtttg ccagcaggaa gcgcgcggtg ttacggatac gacgatagct atcggcagca
    63001 cgtttcagga tctcgtcaga aacggccatt tcaccggtgt agtcggttga tgccacccac
    63061 agacgcagaa tatccgcgcc cagtttgttc atcacatcct gcggcgaaac ggtgttgccg
    63121 atggatttag acatcttgcg accctgacca tccacggtaa agccgtgggt cagtacctga
    63181 cgatacggcg ctttgccttt catcgcggtg gagatcatta gggaagacat gaaccaacca
    63241 cggtgctggt cagaaccttc cagatacatg tccgctgcgt gaccggcaaa ttccggacgc
    63301 acgtcaacaa cagaagagtg ggtagatccg gagtcaaacc atacgtcgag agtatccggc
    63361 actttcacat actggtcagc ttcgtcgccg aggatctctt tcgcatcgag atcccaccac
    63421 gcctggatgc cgtcaacttc aacgcgttta gccacttctt ccatcagttc gagggtacgc
    63481 ggatgcagct cttccgtgtc tttgtgcacg aacagtgaca tcggtacgcc ccaggtgcgc
    63541 tgacgggaga tacaccagtc aggacggtta gcaaccatcg actcgatacg cgcctggccc
    63601 cagtccggga gccactgcac gcctttgatc tctttcagtg actgcgcacg cagacctttc
    63661 tgatccatgc tgacgaacca ctgcggtgtc gcgcggaaga tgatcggcgt tttgtgacgc
    63721 cagcagcacg gatagctgtg ctgcattttc tcaacgtgca gcagcgcgcc tttttcctgc
    63781 agcagcgcaa cgacgatgtc gttcgctttg aagacgttca cgccatccag cgtcggataa
    63841 gtgcccggca gataagtgcc gtccgggcca accgggttag cggtttccag gccgtatttc
    63901 tgaccgatca catagtcgtc cgggccgtgg ccaggcgcgg tgtgaacggc accggtaccg
    63961 gcatccaggg taacgtgatc gccgaggatt gccggaacgt cgaagcccat aaacggatgg
    64021 gtaaagcgca gcagctcaag ctctgcacct tttaccgtgc cgagaatggt gtaatcggtc
    64081 acgccgatac gctgcattac gctttcaacc agatctttcg ccagaatcac ggcctgaccg
    64141 tcgatctgca ccagcgcata gtcgaaatca ggtgcaatag agattgcgcg gttcgcaggc
    64201 agagtccacg gcgtagtggt ccagatcacc agcgagattg ggccgttaac gttgcttacg
    64261 ccaaatttcg ctttcagcgc atcctgatcg accgcctgga aagcgacgtc aatggacgga
    64321 gaagttttgt cgtaatactc aacttccgct tctgccagtg cagaacggca gtctacgcac
    64381 cagtgcaccg gcttcgcgcc tttgtgcagg tgaccattgc cgatgatttt gcccagcgcg
    64441 cggatgatgt tggcttcagt tttgaagtcc atagtcagat acgggtgcga ccagtcgccc
    64501 agcacgccca gacggataaa gtctttgcgt tgaccgtcaa cctgggtcgc cgcgtattcg
    64561 cggcacttgg cgcggaactc ggcggcggtg aatttctcac ccggcttacc gtattcttgc
    64621 tctactttca gctcgatcgg cagaccgtgg cagtcccagc caggcacata cggcgagtca
    64681 taaccggaaa gccctttgga cttcacgata atgtctttca gaatcttgtt aaccgagtga
    64741 ccaatatgaa tgctgccatt cgcataagga gggccatcat gcagaatgaa ggtttttttg
    64801 ccttttttag ccgcacggat gatgccgtac agatcatcat cagtccaacg cgccagcatt
    64861 cccggttcgc gcttggcgag atcgccacgc atcgggaacc ctgtttccgg caaattcagg
    64921 gttgatttat agtcactcat cagattctcg gttccgtgtt tcggtttgat tacataacag
    64981 gcttaagccg gttttgttag cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc
    65041 tgcgctttca gttcgtccag cgacgcaaat cgctgctcat tgcgtatttt tttacgcagc
    65101 actacttgta tatggcgacc gtaaaggtcc attgcaacat ctaacaaatg cacttccagc
    65161 tgctggcgaa taccggcaac cgttgggcgt gttccgatgt ttgccacgcc gggtaacggc
    65221 ttttcaccga ggcccagcac ttctaccgca taaacccctt tcaccgggga aacctgacgg
    65281 cgtagcggta cattcgccgt cgggaaacct atagtgcgcc ctaattcatc accgtggact
    65341 acacgcccgg agatggcaaa cgggtgcccc agtaaacttt ctgccagagc cagattgtca
    65401 tcagccagcg cctgacgcac ggcggtgctg ctgatacgca caccaccttc gcaaaaagtt
    65461 tgcgtactgg tgatatcgaa gccgtattcc atgccagctt tctgtaataa caagaaatcg
    65521 ccttcacgac cagcgccaaa gcggaaatca tcacctacgg caagaaattt tacgcgcaaa
    65581 tgcttcacca aaagatcgct gatgaaattt tgcgcggtta acgccgcaaa acgcctgtcg
    65641 aaacgcacgc acagcacgta atcaacgcca cactctgcaa ggtaacgcag tttttcccgc
    65701 agccgggtca gtcttgccgg ggctttatcg gtagcaaaca gttccagtgg ttgaggttca
    65761 aaaagcatca ccatcaccgg taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac
    65821 agcgcgcgat gaccgcgatg cacgccgtcg aaattaccaa tagtcagcac acacccttct
    65881 tgcggggcct ggctgagatt atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag
    65941 tgaaaatcgt ccgagtatac cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga
    66001 aatcagaaag aagtggcttt tcattgccat ggagcaaatc acgggaagaa actgaccgcc
    66061 tgctgcaatt tttatcgcgg aaaagctgta ttcacacccc gcaagctggt agaatcctgc
    66121 gccatcacta cgtaacgagt gccggcacat taacggcgct tatttgcaca aatccattga
    66181 caaaagaagg ctaaaagggc atattcctcg gcctttgaat tgtccatata gaacacattt
    66241 gggagttgga ccttggctaa tatcaaatca gctaagaagc gcgccattca gtctgaaaag
    66301 gctcgtaagc acaacgcaag ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac
    66361 gcagctatcg aagctggcga caaagctgct gcacagaaag catttaacga aatgcaaccg
    66421 atcgtggacc gtcaggctgc taaaggtctg atccacaaaa acaaagctgc acgtcataag
    66481 gctaacctga ctgcacagat caacaaactg gcttaatcgc caatttgctg aagctttgtg
    66541 aaaaagcccg cgcaagcggg tttttttatg cctgctgctt ttgtgatgcg tgagagaaca
    66601 cactgccggt tgccagatag tcaaaagaga catgccgttg tactatctga actcttcgtg
    66661 gcttaagaat cgtagaacca tcggctgata cattctgaac aagaatatct actcaatctc
    66721 cagactcccc cacaaagaat atggatattg tgatacacat tgaggtatta aacttaagac
    66781 cactctattt ttgaattaaa ttcctatata ctttaaccat tcataaatat ctcctcatta
    66841 gacctt
//
LOCUS       DAJTPG010000008        66306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTPG010000008 DAJTPG010000000
VERSION     DAJTPG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..2153)
                     /gene="glgP"
                     /locus_tag="OO230_000578"
     CDS             complement(<1..2153)
                     /gene="glgP"
                     /locus_tag="OO230_000578"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5690457.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKD"
     gene            complement(2172..3605)
                     /gene="glgA"
                     /locus_tag="OO230_000579"
     CDS             complement(2172..3605)
                     /gene="glgA"
                     /locus_tag="OO230_000579"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5690458.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3605..4900)
                     /gene="glgC"
                     /locus_tag="OO230_000580"
     CDS             complement(3605..4900)
                     /gene="glgC"
                     /locus_tag="OO230_000580"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5690459.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(4918..6891)
                     /gene="glgX"
                     /locus_tag="OO230_000581"
     CDS             complement(4918..6891)
                     /gene="glgX"
                     /locus_tag="OO230_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5690460.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(6888..9074)
                     /gene="glgB"
                     /locus_tag="OO230_000582"
     CDS             complement(6888..9074)
                     /gene="glgB"
                     /locus_tag="OO230_000582"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5690461.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9347..10450)
                     /gene="asd"
                     /locus_tag="OO230_000583"
     CDS             complement(9347..10450)
                     /gene="asd"
                     /locus_tag="OO230_000583"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5690462.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10642..11235
                     /gene="yhgN"
                     /locus_tag="OO230_000584"
     CDS             10642..11235
                     /gene="yhgN"
                     /locus_tag="OO230_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5690463.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11292..12632)
                     /gene="gntU"
                     /locus_tag="OO230_000585"
     CDS             complement(11292..12632)
                     /gene="gntU"
                     /locus_tag="OO230_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5690464.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12636..13163)
                     /gene="gntK"
                     /locus_tag="OO230_000586"
     CDS             complement(12636..13163)
                     /gene="gntK"
                     /locus_tag="OO230_000586"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5690465.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13302..14297)
                     /gene="gntR"
                     /locus_tag="OO230_000587"
     CDS             complement(13302..14297)
                     /gene="gntR"
                     /locus_tag="OO230_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5690466.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14521..15216)
                     /gene="yhhW"
                     /locus_tag="OO230_000588"
     CDS             complement(14521..15216)
                     /gene="yhhW"
                     /locus_tag="OO230_000588"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5690467.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15339..16376)
                     /locus_tag="OO230_000589"
     CDS             complement(15339..16376)
                     /locus_tag="OO230_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5690468.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16709..17197
                     /gene="yhhY"
                     /locus_tag="OO230_000590"
     CDS             16709..17197
                     /gene="yhhY"
                     /locus_tag="OO230_000590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5690469.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17256..18986)
                     /gene="ggt"
                     /locus_tag="OO230_000591"
     CDS             complement(17256..18986)
                     /gene="ggt"
                     /locus_tag="OO230_000591"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5690470.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSRIITTVLQMVVNSIDYGMNVAEATNAP
                     RFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDP
                     RSVDDLTAGY"
     gene            19106..19546
                     /locus_tag="OO230_000592"
     CDS             19106..19546
                     /locus_tag="OO230_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5690471.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19533..20276)
                     /gene="ugpQ"
                     /locus_tag="OO230_000593"
     CDS             complement(19533..20276)
                     /gene="ugpQ"
                     /locus_tag="OO230_000593"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5690472.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20273..21263)
                     /locus_tag="OO230_000594"
                     /pseudo
     CDS             complement(20273..21263)
                     /locus_tag="OO230_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21265..22110)
                     /gene="ugpE"
                     /locus_tag="OO230_000595"
     CDS             complement(21265..22110)
                     /gene="ugpE"
                     /locus_tag="OO230_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5690473.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22107..22994)
                     /gene="ugpA"
                     /locus_tag="OO230_000596"
     CDS             complement(22107..22994)
                     /gene="ugpA"
                     /locus_tag="OO230_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5690474.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23092..24408)
                     /gene="ugpB"
                     /locus_tag="OO230_000597"
     CDS             complement(23092..24408)
                     /gene="ugpB"
                     /locus_tag="OO230_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5690475.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(24805..25518)
                     /gene="livF"
                     /locus_tag="OO230_000598"
     CDS             complement(24805..25518)
                     /gene="livF"
                     /locus_tag="OO230_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5690476.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25520..26287)
                     /gene="livG"
                     /locus_tag="OO230_000599"
     CDS             complement(25520..26287)
                     /gene="livG"
                     /locus_tag="OO230_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5690477.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26284..27561)
                     /gene="livM"
                     /locus_tag="OO230_000600"
     CDS             complement(26284..27561)
                     /gene="livM"
                     /locus_tag="OO230_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5690478.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27558..28484)
                     /gene="livH"
                     /locus_tag="OO230_000601"
     CDS             complement(27558..28484)
                     /gene="livH"
                     /locus_tag="OO230_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5690479.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28532..29641)
                     /gene="livK"
                     /locus_tag="OO230_000602"
     CDS             complement(28532..29641)
                     /gene="livK"
                     /locus_tag="OO230_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5690480.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30065..30448
                     /gene="panM"
                     /locus_tag="OO230_000603"
     CDS             30065..30448
                     /gene="panM"
                     /locus_tag="OO230_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5690481.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30647..31750)
                     /gene="livJ"
                     /locus_tag="OO230_000604"
     CDS             complement(30647..31750)
                     /gene="livJ"
                     /locus_tag="OO230_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5690482.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32021..32875)
                     /gene="rpoH"
                     /locus_tag="OO230_000605"
     CDS             complement(32021..32875)
                     /gene="rpoH"
                     /locus_tag="OO230_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5690483.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33120..34178)
                     /gene="ftsX"
                     /locus_tag="OO230_000606"
     CDS             complement(33120..34178)
                     /gene="ftsX"
                     /locus_tag="OO230_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5690484.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34171..34839)
                     /gene="ftsE"
                     /locus_tag="OO230_000607"
     CDS             complement(34171..34839)
                     /gene="ftsE"
                     /locus_tag="OO230_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5690485.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(34842..36338)
                     /gene="ftsY"
                     /locus_tag="OO230_000608"
     CDS             complement(34842..36338)
                     /gene="ftsY"
                     /locus_tag="OO230_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5690486.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36488..37084
                     /gene="rsmD"
                     /locus_tag="OO230_000609"
     CDS             36488..37084
                     /gene="rsmD"
                     /locus_tag="OO230_000609"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5690487.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37074..37343
                     /locus_tag="OO230_000610"
     CDS             37074..37343
                     /locus_tag="OO230_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5690488.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37346..37705)
                     /locus_tag="OO230_000611"
     CDS             complement(37346..37705)
                     /locus_tag="OO230_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5690489.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            37846..38472
                     /locus_tag="OO230_000612"
     CDS             37846..38472
                     /locus_tag="OO230_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5690490.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38546..40744
                     /gene="zntA"
                     /locus_tag="OO230_000613"
     CDS             38546..40744
                     /gene="zntA"
                     /locus_tag="OO230_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5690491.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(40846..41091)
                     /gene="tusA"
                     /locus_tag="OO230_000614"
     CDS             complement(40846..41091)
                     /gene="tusA"
                     /locus_tag="OO230_000614"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5690492.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41312..41977
                     /locus_tag="OO230_000615"
     CDS             41312..41977
                     /locus_tag="OO230_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5690493.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42050..42607
                     /gene="dcrB"
                     /locus_tag="OO230_000616"
     CDS             42050..42607
                     /gene="dcrB"
                     /locus_tag="OO230_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5690494.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42611..43828)
                     /locus_tag="OO230_000617"
     CDS             complement(42611..43828)
                     /locus_tag="OO230_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5690495.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            43962..45011
                     /locus_tag="OO230_000618"
     CDS             43962..45011
                     /locus_tag="OO230_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5690496.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45066..45653
                     /gene="acpT"
                     /locus_tag="OO230_000619"
     CDS             45066..45653
                     /gene="acpT"
                     /locus_tag="OO230_000619"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5690497.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            45764..47338
                     /gene="nikA"
                     /locus_tag="OO230_000620"
     CDS             45764..47338
                     /gene="nikA"
                     /locus_tag="OO230_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5690498.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47338..48282
                     /gene="nikB"
                     /locus_tag="OO230_000621"
     CDS             47338..48282
                     /gene="nikB"
                     /locus_tag="OO230_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5690499.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48279..49112
                     /gene="nikC"
                     /locus_tag="OO230_000622"
     CDS             48279..49112
                     /gene="nikC"
                     /locus_tag="OO230_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5690500.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49112..49876
                     /gene="nikD"
                     /locus_tag="OO230_000623"
     CDS             49112..49876
                     /gene="nikD"
                     /locus_tag="OO230_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5690501.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            49873..50679
                     /gene="nikE"
                     /locus_tag="OO230_000624"
     CDS             49873..50679
                     /gene="nikE"
                     /locus_tag="OO230_000624"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5690502.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50685..51086
                     /gene="nikR"
                     /locus_tag="OO230_000625"
     CDS             50685..51086
                     /gene="nikR"
                     /locus_tag="OO230_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5690503.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51206..51565)
                     /locus_tag="OO230_000626"
     CDS             complement(51206..51565)
                     /locus_tag="OO230_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5690504.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51562..51837)
                     /locus_tag="OO230_000627"
     CDS             complement(51562..51837)
                     /locus_tag="OO230_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5690505.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(51896..53020)
                     /locus_tag="OO230_000628"
     CDS             complement(51896..53020)
                     /locus_tag="OO230_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5690506.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53020..55755)
                     /gene="rbbA"
                     /locus_tag="OO230_000629"
     CDS             complement(53020..55755)
                     /gene="rbbA"
                     /locus_tag="OO230_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5690507.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(55752..56819)
                     /locus_tag="OO230_000630"
     CDS             complement(55752..56819)
                     /locus_tag="OO230_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5690508.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57185..58807)
                     /locus_tag="OO230_000631"
     CDS             complement(57185..58807)
                     /locus_tag="OO230_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5690509.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59069..60658)
                     /locus_tag="OO230_000632"
     CDS             complement(59069..60658)
                     /locus_tag="OO230_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5690510.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61043..62074
                     /locus_tag="OO230_000633"
                     /pseudo
     CDS             61043..62074
                     /locus_tag="OO230_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62161..62637)
                     /locus_tag="OO230_000634"
     CDS             complement(62161..62637)
                     /locus_tag="OO230_000634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690511.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62630..63394)
                     /locus_tag="OO230_000635"
     CDS             complement(62630..63394)
                     /locus_tag="OO230_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5690512.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63640..64401
                     /locus_tag="OO230_000636"
     CDS             63640..64401
                     /locus_tag="OO230_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5690513.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64508..65008)
                     /locus_tag="OO230_000637"
     CDS             complement(64508..65008)
                     /locus_tag="OO230_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690514.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65159..>66306)
                     /locus_tag="OO230_000638"
     CDS             complement(65159..>66306)
                     /locus_tag="OO230_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5690515.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16108 a        17798 c        16274 g        16126 t
ORIGIN      
        1 tcatctttct cgtagtattc acgcggtttg tagccctgac gacgcagttc ttccacttct
       61 tccgctgtgt taccaaaaat aaagatattg tcagcaccga catgatccag catctcgaca
      121 ttcgcaccgt ccaacgtacc gatagtcagc gcaccgttaa gcgcaaactt catgttactg
      181 gtgccggaag cttccgtccc tgccagcgaa atctgttcag acagatctgc cgccggaatg
      241 atcaactgcg ccaggctaac gctgtagttc gggatgaaca cgactttcag cttatcgcca
      301 atctgcggat cgttgttgat cactttcgct acgtcattga tcaaatgaat aatgtgcttc
      361 gccatgtaat aggccgaagc cgccttaccg ccaaaaatat tcacgcgcgg tacccacttc
      421 gcatccgggt cggccttgat gcggttatag cgggtaatca catgcaacac attcatcaat
      481 tgacgtttgt attcgtgaat acgtttgatt tgtacatcga acaacgcctt tggattcacc
      541 accacattca gctgctgggc gatatactct gccagacgct ttttgttctc cagcttcgcc
      601 tgatgcacag cgtgattaac cattgggaaa tcacagtgtt gttgcagctc attaagcagg
      661 ctaaggtcgg tgcgccagtt acggcccagg tgttcgtcca gcacggctga aagcgatggg
      721 ttcgctaccg ccagccagcg acgcggcgtc acaccgttgg tgacgttggt gaaacgaccc
      781 gggaagattt tcgcaaagtc ggcaaacaac gattgcacca tcagattaga gtgcagttcc
      841 gatacaccgt taactttgtg gctcacaaca accgccagcc aggccatacg cacacgacga
      901 ccgttggatt catcaatgat cgacgcccgt cccagcagat cggtatcgtt cggatactgt
      961 tcctgcaagg ttttcaggaa atagtcgttg atttcaaaga tgatctgcag gtgacgcggc
     1021 agaattttac ccagcatatc aaccggccag gtttccagcg cctcgctcat cagcgtgtgg
     1081 ttagtgtagg agaagacctg acaacacacc tcaaacgcgt cgtcccagct aaattggtgc
     1141 tcatcgatca gcagacgcat catctcagga atcgacagta ccggatgggt atcattgaga
     1201 tgaatcgcga ttttatccgc caggttatcg taggttttat gcaactgata atgacggctt
     1261 aaaatgtcct gaatggtcga ggaaaccaag aagtattcct gacgcaggcg cagctcacgc
     1321 ccggagtagg tggagtcatc cggatacagt acgcgagata cgttctcgga gtggttttta
     1381 tcttccactg ccgcgaagta gtcaccctgg ttgaatttac cgaggttaat ttcgctactg
     1441 gcttgcgcac tccacaaacg cagcgtgttg gtcgcgtcgg tgtcgtaacc agggattatc
     1501 tgatcgtaag cgactcccag aatctcttcg gtttcaatcc agcgcgtttt tttaccttcc
     1561 tgctgaatgc gaccaccaaa acggacttta tagcgcgtgt tgtggcgttt gaattcccac
     1621 gggttaccgt attccagcca gtagtctggc gactctttct ggctaccgtt aacgatgttc
     1681 tgcttgaaca taccgtagtc atagcggatg ccgtaaccgc gccccggcaa ccctaacgtc
     1741 gccagagaat caaggaagca agccgccaga cgtcccaggc caccgttacc gaggcctggg
     1801 tcattttctt catcaatcag ctcttcgaga tttaacccca tcgcttccag tgcgccctgt
     1861 acatcttcgt aaattcctag cgacaacatg gcgttggaga gcgtacggcc aatcaaaaac
     1921 tccatcgaca ggtagtaaac ctgacgagtt tcttgcgaca actgggcacg gtttgaacgt
     1981 aaccagcgct ccacgagacg atcgcgcaca gcaaataacg ttgcgttcag ccattcatgt
     2041 ttattggcga cgaccgggtc ctttccaatc gtaaacatca gcttgtaagc gatagagtgc
     2101 ttaagagctt ctacgctaag cgtgggcgat gaatatgtaa acggagcatt catataggcg
     2161 tttcctgaaa actatttcaa gcgatagtaa agctcacggt acgacttcgc cgcgacctgc
     2221 cagctaaaat ccattgccat agcctgacgt tgcacaaacc gccacagtga aggacgggac
     2281 cacagtacaa aagcacgccg aatagcccgt aacagcgacc aggcattact atcttcaaag
     2341 acaaacccac tggcgacgcc atctgcaagg ttctcaagag aacagtcaga aaccgtatca
     2401 gcaagcccac cggtgcgccg cactaacggc agcgtaccgt acttcaatcc ataaagttgc
     2461 gttaagccgc acggttcaaa acggctgggc accagaatga cgtccgcgcc gcccataatg
     2521 cgatgcgaaa atgcttcgtg atagccaatc tgaacgccca cctgaccggg gtattccgct
     2581 gccgccgcaa ggaaaccttc ctgcagcacc ggatcgcccg cgccgagtag cgccagctgc
     2641 ccgccctgct ccagaagccc cggtaaggct tccagcacca ggtcgagacc tttctggctg
     2701 gtcagacggc tcaccactgc aaaaagcggc actttatcgt caaccttaag ccccattgcg
     2761 atttgtaact ggctcttatt ttccgcttta tcttccaacg tatcgcgggt gtaacgcgag
     2821 gccaacagta agtccgtctc tggactccag attttctcgt ccacgccgtt cagtacgcct
     2881 gaaagacgcc cttcacggtg acgctgttgc aacagacctt ccataccgta ggcaaactgc
     2941 ggttcggtga tctcgcgagc gtaggttgga ctgaccgccg taatgtgatc ggcatagtac
     3001 agaccggcct tcaggaaaga gatttgtccg ttgaattcca gcccatgaat attaaagaat
     3061 gaccatggca attggatgtc attcatgtga tgtgcataaa acatgccttg ataggccagg
     3121 ttgtgcacag taaacaccga cttcgcagga cgcccgcgcg ccgccagata cgcaggcgca
     3181 agacctgcat gccagtcgtg cacatgcacc acatcaggac gccagaatgg gtcaagcccg
     3241 ctggccattt ctgccccaac ccaccccagc agcgcaaaac gcaatacgtt gtcggtatag
     3301 gcaaataagt tggtatcgtg atacgggctt cccggacgat catagagatg cggcgcgtca
     3361 atcaggtata tgccaacccc gttgtaatga ccgaacaaca gcgtgatatg cccggcgaaa
     3421 gtatcacgac gggatactac ctgcgcatcg gtcacgccac ggcgaatatc gggaaatgca
     3481 ggcaacagta cgcgagcgtc aacgccgtct gcgatttgtg ctgcgggtaa tgccccaata
     3541 acatcagcca gaccgccggt tttaagcagc gggaacatct ctgaacatac atgtaaaacc
     3601 tgcattatcg ctcctgttta tgccctaact tccgtagcat ttcgcgcgtt accagcacga
     3661 tgccttcttc tgaacgatag aaacgacgtg catcttcctc tgcgttttca ccaatcacca
     3721 tgccttccgg gataacacaa gcacgatcga tgacgcagcg gcgcagacgg cacgagcgac
     3781 ctacccatac ttccggtaac aatacggcgg aatcaatgtt gcagaatgaa ttcacgcgaa
     3841 cgcgcgagaa cagaacggac tgcaccacca ccgaaccgga gatcacacaa ccgccggata
     3901 ccagtgagtt aagggtcatc ccgtggctac cggagcgatc ctgcacgaat ttcgctggcg
     3961 gtaatgattc attgtaggtg cgaattggcc aattgcgatc gtacatatcc agttccggca
     4021 ccacagaggc cagatcgaga ttcgctttcc agtaagcttc cagcgtaccc acatcgcgcc
     4081 agtacggctc ggcatccggg tcggattgta cgcaagagag cgggaacggg tgcgcatagg
     4141 ccagaccggc ttcggtgatc ttgggaatca aatctttgcc aaagtcgtgg ctggagttct
     4201 catcacgatc gtcttcttcc agcagttcat acagatagtc ggcgtcaaag acgtagatac
     4261 ccatactcgc cagagatttg ctcggatcgt tcggcattga cggtgggtta gcaggttttt
     4321 caacgaattc gataatttta tcgttctcat caaccgccat aacgccaaat gcggaggctt
     4381 cttcaatcgg tactggcata caagcaacgg tgcaacgtgc gcctttttcg acgtgatcga
     4441 taagcatacg cgagtagtct tgcttgtaga tatggtcgcc cgccaggatc accacgtatt
     4501 ccgctttata acggcggata atgtcgaggt tttgggtgac cgcatctgcg gtgccgcgat
     4561 accagttttc ccctttcatt ctctgctgtg ctggcagcag atcgacaaac tcgttcattt
     4621 cttcattgaa gaatgaccag ccgcgctgaa tgtgctgcac cagagtgtgg gactggtact
     4681 gggtgatcac gcccatacga cggatcccag agttgatgca gttagacagc gcaaagtcaa
     4741 taatgcggaa cttaccgccg aagtgtacgg ccggttttgc tcgcttattg gttaaatcct
     4801 tcaggcgggt accacgtcct cccgccagta tcagggcaac agatttcaat ggcagctggc
     4861 gcgccaacat taagtgatcg ttcttctcta aactaaccat gactaactcc ttttttatca
     4921 tctctggaac acacacaatc cgtgtgcagg tccctgccag acaaccgtaa tcactgggtt
     4981 atcctctcca gcgaatgggg gaatggcgtg ccactcccca gcaggtaaaa caatctctgt
     5041 tacctcaagc gtggcgttaa ttgcgatcaa aaagcgatcc gagagcagaa tttgcagctg
     5101 tttcggcccg ttttgccact catccgtgct taaaggttga gcatatcgat ttagccaacg
     5161 gacattgccg tcaccttctt cccaccagcg attctccacc aaagcgggaa tgcgcttgcg
     5221 cagatggatt aacgcggcgg taaatgcggt taaaccactg cttgcctgcg accagtccaa
     5281 ccagtttaat tggttatcct gacagtaggc attgttattg ccatgctggc tgtgaccatg
     5341 ttcgtcaccg gccagtaaca tcggcgtgcc ctgggagagc aacaacgttg ttaacagggc
     5401 atgaatgctg tcgcgccgcc gttcaaccag gtcaagagta ccgcctaacc cttctttacc
     5461 atgattgtta ctgtaattgt tgttggtccc gtcgcgattt tcttctccgt ttgcttcatt
     5521 gtgtttatgg ttgaagcaaa cgcagtcgcg aagcgtaaaa ccgtcatgcg cggtgacgag
     5581 attaatcgcg gcactcggca gacgaccatt acgtttaaaa acatcgctgg aggcagcaaa
     5641 acgcccggca aacgccccca gaggcaaatc ataatgtagc cagaaacgac gggcagcatc
     5701 gcggaaatga tcgttccact cggcaaacag cggcgggaaa tttcccacct gataaccact
     5761 aggagcgata tcccacggtt cagcaattaa cttcacctgc gagagcaccg ggcagttctg
     5821 gatagcggta aacaacggcg catcctgacg gaactctggc gtacggccca tgactgccgc
     5881 cagatcaaag cggaaaccat cgacgtggca ggtttctacc caataacgca ggcaggcgct
     5941 ggcataatcc accaccgccg gatgactcaa attgagcgtg ttgccgcaac cggtccagtt
     6001 gtgataatcg ccgtcttctc ttatccaata atagctacgg ttatcgatcc cacgcagcga
     6061 gaataacggg ccgtcgaggt ccagttccgc actatggttg agcacgatat caagaatgac
     6121 ttcgataccc gctttatgca gtgctttgat tgcatcgcga aactcatcca gcgccgtttc
     6181 tggcgagcag gcatacgccg gatgcagcgc aaacatcgcc accgggttgt aaccccagta
     6241 gttacttagc cccatgcgtt gcagacgtgg ttcactggca aactgcgcca ctggcagcag
     6301 ttccagcgcg gtaatgccca attgtttcaa atagttgatc atcaccggat gcccgagggc
     6361 tttataagtg ccacggatct cgaccgggat ctccgggtgc aagtacgtta atcctttgac
     6421 atgggcttca taaatgatgg tgctgcccca cggcgtgcgc ggcggggcgt catcttccca
     6481 gtcatagtga tcaaccacca ctacgcattt cggcgcaatg gcggcgttgt cgcgatagtc
     6541 aggttcatta tgaccggcgt gcagcagcgg gttatcttta aactccccgt caatttgccg
     6601 cgcgcaagga tcaatcaaca acttcgccgg gttaaagcga tgcccctcgg cgggttgcca
     6661 ggggccatga acgcgataac cataacgcaa acccgggcgc gcatccggca gataaccgtg
     6721 ccaaatgtcg ccactgttcc ctggcaagtc atagcgatgt tcctggccat tggcgtcaaa
     6781 gacacacagt tctacccgct cggcatgagc ggagaaaagt gtgaagttga cgccctgacc
     6841 gtcgtaatgc gcgccgaggg gagcgggttt gccaatggcg agttgtgtca ttctgcctcc
     6901 cgaaccagcc agatagtggc cagcggtggt agcgtcaggc ttagtgaatg ctgacgaccg
     6961 tggctggcaa tctcatcgct gtgtaccgtg ccgccattgc ctgcattact gccgtgatag
     7021 tgtatggaat cggtattgag gatttcacgc catttacctg gctgattaat gccgaagcga
     7081 taatcatgac gcggcaccgg cgtaaagtta ctggcaacga taatttcgtt accctcttta
     7141 tcgcgacgca caaagatcag caccgagcgt tctttgtcat ccaccaccag ccattcaaag
     7201 ccgtacgggt caaaatccag ttcatgcatt gctttatggt ggcggtaggt gaggttcaga
     7261 tcgcgcacca gacgctggac accgtggtgc cagttatcgc cgccttccaa cagatgccag
     7321 tcgaggctgg cgtcatggtt ccactcgcgg ccctgggcaa attcgttccc catgaacagc
     7381 agtttcttgc ccggaaatgc ccacatccag ccatagtaag cgcgcaggtt agcgaatttc
     7441 tgccatgcgt cgcccggcat gcggtcgaga atcgattttt taccgtggac cacttcatca
     7501 tgcgacaacg gcaggacgaa gttttcagtg tagttgtaga gcatcccgaa ggtcagttta
     7561 tcgtgatgat actgacgata aatcgggtcg agcttcatgt agtccagggt gtcatgcatc
     7621 cagccgaggt tccacttgta ccagaagccc agaccgccca tatcctgcgg acgagaaacg
     7681 ccagggaaat cggtagactc ctcagccatt gtcaccgcac cggaaacctg ctcaccaaga
     7741 atacggttgg tattacgcaa gaattcaatc gcttcaagat tctcgcgccc gccaaattcg
     7801 ttcgggatcc actccccctc tttacggctg tagtcacgat aaatcatcga cgccaccgca
     7861 tcgacgcgca gcgcatcaat accaaaacgt tcaatccagt aaagcgcgtt accgacgagg
     7921 aagttactga cttcacggcg accatagttg tagatcagcg tgttccagtc ctgatgatag
     7981 ccttcacgcg gatcgctgtg ttcatacaag ttcgtgccat caaattcggc aagcgcaaag
     8041 tcatcagtcg ggaagtggcc tggcacccag tcgagaatca cgttcagacc agctgcgtgt
     8101 gcggcatcaa tgaaataacg gaagtcgtcg cgagtaccaa aacggcgggt tggtgcatac
     8161 aggccggttg gctgataacc ccaactgcca tcgaagggat gctcgttaat gggcagtagt
     8221 tcgaggtggg taaagcccat ccatttagca taaggcacca gttgatcggc cagctcgcgg
     8281 tagctcaacc agaaattgtt gtcggtgtgg cgacgccagg aacccaggtg aacttcataa
     8341 atagagattg gcgcatcaaa ctgattcgct tttttgcgct cttcagtctg tacaaccttt
     8401 tccggcagcc cgcaaataag agacgcggtt tccgggcgca tttgcgcttc gaaggcataa
     8461 gggtcggact tcagacgcaa gttgccattg gcatcaatca tctcgtattt atagagctga
     8521 ccgttatgcg ccccagggat aaacagttcc cagatgccgc tctctttacg caggcgcatc
     8581 gggtgacggc gaccgtccca gtagttgaat tgcccaacca ccgagacccg acgggcgttt
     8641 ggagcccaga cagagaaacg cgtacctgtg acgccatcca tagtatctgc atgcgcgcct
     8701 aaggtttcat acgggcgcag gtgagtacct tcagataata gccaggcatc catttcctgg
     8761 attagcggac caaaacggta aggatcatca atcaggtttt gctgaccatg ccagacaaca
     8821 gccaactgat agcggaaaaa attcttacgt cgcggaatga cgccgctaaa gaatccccgt
     8881 gagtcgagac actccagttt tgcgagtttg cgcccggttt tcggttcaat cacccacaca
     8941 tcggtagcgt cgggtaaaag ggcacggact tccagtcccg cggtggtttt atgcattccc
     9001 agtacggaaa aaggatccgc aaaatggcct gcaattagcg cgttaatcac gtctctatcg
     9061 atacgatcgg acatgcttgt cttcctgttt tattgtgtca ccccatccaa ttggattttt
     9121 gacttctggt tgtgacattt ttttgacctg aacggcgcag cactctgtgc atcctctctg
     9181 cgtcgtcctc acttcaggta aggctgtgaa tactcatgta ttcagccacc cttaaagaat
     9241 agccaatgct ctatttaact cccggtaaat catgaaacat ctgcgcttac tcctgtatta
     9301 cgcactaaca ggggcggcat cgcgccccag atttaatgaa taaagattac gccagttgac
     9361 gaagcatccg acgcagcggc tccgcggccc cccacagcag ctggtcgccc acggtaaagg
     9421 ctgacaggaa ctctggtccc atattcagct tacgcaggcg gcctaccggc gtggtcagcg
     9481 tgccggtaac ggcagctggg gttagctcac gcatagtgat ttcccgatcg ttcggaacga
     9541 ctttcgccca cggattgtgc gcagccagca gttcttcgac ggtcggaata gacacatctt
     9601 ttttcaattt aatagtgaat gcctggctgt ggcagcgcaa tgccccgaca cgcacacata
     9661 aaccatctac cggaattacg gaagatgtgt tgaggatctt gttggtttcc gcctgccctt
     9721 tccactcttc gcggctctga ccgttatcga gctgtttgtc gatccacgga atcaggctac
     9781 ccgccagcgg cacgccaaag ttatccaccg gcagctcacc gctacgggtt aaggttgtga
     9841 ctttgcgttc gatatcgaga atagcagagg acggggtcgc gagttcatct gccacatggc
     9901 catacagatg gcccatctgg gttaataact cacgcatatg tcgcgcacca ccgccggaag
     9961 cggcctggta ggttgcaacg gacacccaat caacaagatc attggcgaat aaaccaccca
    10021 acgacatcaa catcaggctt acggtacagt taccgccaac aaaagtcctg atgccattat
    10081 ttaatccgtc ggtaatgacg tcctgattga cggggtcaag aatgatgatg gcgtcatctt
    10141 tcatgcgcag agacgatgct gcgtcaatcc agtaaccttg ccatccgctt tcacgaagct
    10201 ttggatagat ttcgttggta taatcgccgc cctgacaggt cacaatgata tcgagggcct
    10261 ttagcgcctc tagatcaaag gcatcctgaa gtgtgccagt ggttccgcca aaagacggcg
    10321 cagcctggcc aagctgagaa gtagaaaaga agacagggcg aatggcgtcg aagtcgcgct
    10381 cttcaaccat gcgttgcatg agaacggagc cgaccatacc gcgccagccg ataaaaccaa
    10441 catttttcat aagcgttttt tcctgcaaag atgtgtgctg tataaatgtg ccggtctcct
    10501 cttggcacat ctttcaccat acaaaaagct gccaaagtcg caagtgaaat taatcaatga
    10561 tagcgaagcc atcagtaatg cgacttatcc tgctttgtta gcacgcagaa agtccgcggc
    10621 aattatcagg gaatttgagt tatgaatgaa atcatttctg cagcagtttt attgatcctg
    10681 attatggatc cgctcggaaa cctgcctatt ttcatgtccg tactgaaaca tactgaaccg
    10741 aaaagacggc gggcaatcat ggtgcgagag ttgcttattg ctctcctggt gatgctggtg
    10801 ttcctgtttg cgggtgagaa aattctggca tttctcagcc tgcgagcaga aaccgtctcc
    10861 atttctggcg gcatcattct gtttctaatc gccattaaga tgattttccc cagcgcttca
    10921 ggaaatagca gcgggcttcc ggcaggtgaa gagccattta tcgtgccgtt ggcaattccg
    10981 ttagtcgccg ggccgactat tctcgccacg ctgatgttgt tgtctcatca gtacccgaat
    11041 cagatgggac atctggtgat tgctctgctg ctggcctggg gcggcacctt tgtcatcctg
    11101 ctacagtctt cgctattttt acgtctgctg ggcgagaaag gggtgaacgc acttgaacgc
    11161 ctgatgggat tgattctggt gatgatggca actcagatgt tcctcgacgg cattcgaatg
    11221 tggatgaagg ggtaacgagg agagtttttc gaaaagagcc cggtagcgtc acaactaccg
    11281 ggcgaacaaa cttaactcaa cagctggaac gcaatcatcc caacgatggc accaacagtg
    11341 ccgaggatgg tttccatcat ggtccaggtt ttcagcgttt cggcttcggt cgcgccggta
    11401 aatttaccga acaaccagaa accagcgtcg ttaacgtggc tgacaacaat cgaaccacca
    11461 gcgatacaaa tcgacagtgc cgccatttgc gcaccagagt agttcagttg ttcaatgact
    11521 ggcatcacca gtcccaccgc cgttaaacag gctacggtgg cagccccctg aatgatgcgc
    11581 actgcagcag ccagcacgaa gcaggtgata gcaatcggca ggcccatgcc ggttaacgct
    11641 tcgcccagtg ccggacctac gccagagtca accagcacct gtttgaacac gccgcctgca
    11701 ccaatcacca gcagaatgat ccccgccggt tgcagcgcgt gaccgcaaat ctccatcact
    11761 ttgtcttttg gcatgccctg acgcattgcc aggccataaa tcgctaccag acaagcaacc
    11821 agaatcgcgg taaacggatg accaataaac tcgaaccatt cgtaagcggt agagccttct
    11881 ggcacaaaac gcgcggcaat ggttttcagc cccaccagca ccagcggcaa caggatcagc
    11941 gacaggctga atccgaaaga tggcatttta ccttcgccga gatgcggttc gctgatgtcg
    12001 tcaggaatat gcagctcaac gtaacggctg atgaagttac cccacagcgg cccggcaata
    12061 atcattcccg gaattgccgc acacaggcca atcaggatca tccagccaaa gtcggcattc
    12121 atctgcgatg ccagcagcat tggcgctggc ccaggcacca ggaacgctgc cgcggccgcc
    12181 acgcctgcga ataatgggat taccagcttc accaggttcg taccggtgtg gcgcgccatt
    12241 gagaaagcaa cgctaatcag cagaacaatc gccacttcaa agaacagcgg tagcgcacag
    12301 accagccccg caaggccgat ggcataatgc gcgcggctgt gaccgaagga tttgagcatt
    12361 ttgacggcaa tctgatcgac tgcgccggtt tcatataaga tcttgccaaa catagctccc
    12421 agggcgacaa ccaccgccag gaagccgagg gtgcctccca tccctttttc catcgtcgct
    12481 gcgattttat cgagcggcat accggaaaaa aggccagccc ccatggacac caccatcaaa
    12541 gccaggaaag cgtgcatacg cgccttcatg actaaaaaca gcagcagtaa aacagaccct
    12601 actgctgtta aaacaagcgt taatgtagtc actacttatt tgcctttttt aataacctca
    12661 atggtgcttg ccacaacacc ttccagcggt tgatcgatat ccaccaccag tacatcggtt
    12721 tcgtccgcac ccggctcctg cagcgtttca aactgcgtca ccaacatttg ggttttaaag
    12781 aaatggcctt tgcgcgcttt caggcgactt tcaatcacat caaaatcgcc tttcaggtag
    12841 atgaaagaga gattcggatt accttcacgc agcaagtcgc gatagtgctt tttcagtgca
    12901 gaacaggcga tcagcgacac tttattggtg cgctgcatag caaacgcgga gtcgttcagc
    12961 gcctgcaacc agggtttgcg atcgtcgtca ttcagcggtt cgccagacgc cattttttcg
    13021 atattgcgcc gtggatggag gaaatcgcca tcaagaaacg cggcatgaag ttgatgcgcc
    13081 acttcactgg cgaccgcaga tttgccgctg cccgatacgc ccatcaagac gtaaatgtgg
    13141 tgatcatggt tagtcgtgct caaagtggtg cccccacaat acaagaatta aaaatgttac
    13201 gggtaactgt tatcggcaac attgtccagc cggacaatat cagaagcaat atccatccgt
    13261 gccttaagtg tataagtgtg agctacttca aatttgtggg cttaaataga tccgcccggt
    13321 gacaaggtga aacctaaatc taacattttc ggtgtcacag attcgccacg aatacgcgcc
    13381 agcaggcgtt cagcgccaat actgcccatc cgctcacgcg gcgtcagcac gctcgcaagt
    13441 cgtggctcca tcacctgacc aatgtcatga ccgtggaaac cggcaatcgc catatcgtca
    13501 ggaactttta accccagacg ctgacattca aacgccgcgc cgaccgccag atcatcatta
    13561 gtacagaaca cgccatccag ctgcggatat tcccgccgcg cctggcgaat cagttcaata
    13621 ccggaagagt aagaagaaga ttgctcaacc atcacgctat atggcaccag gcctgcatcc
    13681 agcatcgcct gttcgtatcc cttctgtttg atgatagtac gttcgtcgag acgtgcgccg
    13741 agataggcaa tgtggcgatg cccacgagca ataatggcgg tagtcatctg gcgtgccgct
    13801 tcaaagttat caaaaccgac ggcgatatcg aggcacggcg actggctgtc catcaactcc
    13861 accaccggga tccccgccac ttcaatcatc tttaaggtgc gcggcgtgtg ggtacgttcg
    13921 gtgaggatca ggccgtcgat attccaggag agcatggatt cgagacgttc ttgctccatt
    13981 tccggtttat aaccgtagtg cgccagcatg gtctgataac cgtgcgcgtc ggtgacgctt
    14041 tcgattccgc gtaatacttc cgcgaaaacc tggttggtga gagaaggtaa caggacgcca
    14101 atcgcccggc tggtagcgtt agagaggata tcgggcgcac gattgggaat atagcccagt
    14161 tcatcaagag cggcggcaat cttgccgcgt agagcgacgg aaacctgctc cgggttgcgt
    14221 aaaaaacggc tgaccgtcat tttggtcacg cctacacggt cagccacatc ctgaagtacg
    14281 ggtcttttct ttttcatcgt cctgaaggta caaaagagat agattctctt agtttaacac
    14341 ggacgcatca gaaccttccc ggggaaaaca gtgcttgttt acaattatta gatatacact
    14401 aaatcaattg catcgcattg tgctaacgac gcggcaaaac cccggaagcg tgcatcagca
    14461 cgcgaccgga gcgagcgagg aagcctgcaa aaaggcaggc ttcaaagatg ccgttgagtt
    14521 ttaaaccggc ggcagatcga acagtaacac ttcgctgtcg ctatccgcat ggatggagat
    14581 tgcctgctca tcccagattg ccagaccatc gctggtcgag gctttcacgc cgttaatggt
    14641 aacattgcct tttaccacct gaatccagac gcggcgttca gcggcaatct gatgcaccga
    14701 ctgctcatct ttcagcaacg cccaacgata cagttccata tcctgatgca ctttcagcga
    14761 accatctcgc gcatccggcg agagcaccag ctgtttgccc tgcacggcat cgaagcgacg
    14821 ctgttcataa cgcggcgtaa tgccattttc ttcaggcatg atccagatct gatacagatg
    14881 cagacgctcg gtgctgcttg ggttgtactc tgagtgacga atacccgtac ccgcactcat
    14941 aatctggaac tcacccgccg gaacctgctc tttattgccc atgctgtcct gatgctcaac
    15001 agtaccttcc agcacgtagg tcaaaatttc catatcttta tgcgggtgag tgccgaagcc
    15061 ctgccctact tcaatcacgt cgtcgttaat cacgcgcagc gcggagaagc ccataaagtt
    15121 cggatcgtaa tagttggcac aagagaaagt atgccaggag tccagccagc catgatttgc
    15181 atgaccacgt tcatttgctt tgcgtaagta gatcatttgt attcgccccc tgaatgattt
    15241 cgatgggctt agtgtggacc cgatctgcct gggatgatag agggtgaaaa ttgccccctc
    15301 tgttcaaaaa aattgaacta ttcgaggggc catcaaactt aattcgcgag agtcactgtg
    15361 gagggagagg cttgctcaaa tccgcgttca aggatttcca gattggtaag aacttcagat
    15421 tccttgacgt aatttggcgc accgtgggtg atggtttgat acaacgcatc ataaacacgc
    15481 ccgtaatcgc ccatctccgg cttcatctct tctctgaccg tcacaccctc gtcattgaca
    15541 tactccagca caccgaccga atcatccgct gcgaatcccg gttcgcccgg cataatatta
    15601 gccttcaggc tggtttcctg ctggtcgata ccgtatttaa taaacgaacc tttcttaccg
    15661 tgaacgataa atttcggata atcgattttc accagatggc tggttttgac gatggctttc
    15721 aggtcgccat aaaacagttg cgcttcaaag gtgtcgtcag gattggcttt attacgcagg
    15781 ctgcggatgt cataagcgac gtgatccggg cgaccgaaca gagaaataat ctggtccatc
    15841 gtatgcacac caaggccata gaacgcgcca tcctgcggca gtccaggttt ggtttctgcc
    15901 accgggcggt aatagtcaaa atggctttcc acttcgacaa tctctcccag cttgccgctt
    15961 tcaatcgctt ttttcgccgt caggaagcag gaatcaaagc gacgattctg atatggcgtg
    16021 acggtcagcc ctttgctttt cgccagtgca aacagctctt tcgcctgcgc aagtgtcgga
    16081 gtgaacgttt tttcgaccag cacatttttc ccggcttcca gcgcgcgctt cgcgtactcg
    16141 aagtggctgt ccgcatgggt gcagacaaca accaacttaa catcgggatc gtttagcact
    16201 tcgtcgagat cgctggtgaa atggatatga gagtaaatgg gagcctgttc ttccggcttc
    16261 gcatggcgac gaaaaatatg cgcgacatgc cagctatcct tgcggttaag tacatacggc
    16321 agatggtaac gggtggtgct tttgccgaag ccaataaagg cgcagttgat gaccatgatt
    16381 tcgtcctttt taaggtggtt atttacacct tagcgcaaag cggacgtggt tcctactgga
    16441 gtgcgcataa cgaacacaag cactcccgtg gataaattga gaacgaaaga tcaaaaaaaa
    16501 agccagcacc cggctggcta agtaatactg gaagcaatgt gagcaatgtc gtgctttcag
    16561 gttctccgcg agggtcttcc tgatcgcgat acaataataa tcattctcat tcgcacttgt
    16621 ccaacacttt ttgcaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag
    16681 ggacatttac cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac
    16741 acgggattac gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact
    16801 acaggtgcct catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat
    16861 caagcaactc gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca
    16921 acagcgccca cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa
    16981 gaaccgcggc gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt
    17041 acgggtagat cgcattgaac taaccgtgtt tgtcgataac gcgccagcaa ttaaggtcta
    17101 taaaaaattc ggctttgaga ttgaagggac cggtaagaag tacgcattgc gtaatggtga
    17161 atatgtcgat gcgtattata tggcgcgggt gaaatagaga atttgcaatc taagtttgtc
    17221 atttaagccc tcacctaccg ggagggctta agatattaat accccgccgt taaatcatcc
    17281 accgagcgcg ggtcggatgc gccgtacaac tcaccgtccg gcccaaccat aatgctttgt
    17341 gtgctgccca tcgcctcttt cagcgccact ttctgacctt ttgcttccag cagcttgagc
    17401 gtatccgggc taaacccttt ttcgacacgc agctcgtccg gcaaccactg atggtggaaa
    17461 cgcggcgcat tggtcgcttc ggcgacgttc atgccataat cgatgctatt caccaccatt
    17521 tgcagcaccg tggtgataat ccggctaccg gtaaccagcc aggttttacc gtctttcacc
    17581 acaatggtcg gcgacatcga cgacagcggg cgtttgttcg gcccgacggc gttggcatca
    17641 ccgcccacca gcccgtaaac gttcggtacg cccggtttgg cggagaaatc atccatctgg
    17701 ttattaagca gaataccgct ctcacccgcg acaatacccg taccgaaggt ggtgttcagc
    17761 gtataggtca ccgccaccgc gttaccgtct ttatccacta cagagtaatg ggtagtttga
    17821 ttactctcat aaggcgcaag cttgccaggg cgaatctcac tggacggctt cgcttttttg
    17881 atatcaattt gatcggcaat ggatttggca taggctttat tggtcagtgc ctgccacggg
    17941 actttgacaa aatccgggtc gccaagatat tccgagcggt cggcgtaggc gtatttctcc
    18001 gcttctgcca tgatttgcat cgcatcggcg ctgccaaagc cgtatttctt catatcgaag
    18061 ttttccagaa tattgaggat ttgtacgata tggatcccgc cggaggatgg cggtggcatg
    18121 gagtaaacct gatacccgcg ataatcgccg cttatcggag tgcgttcgac cgctttatag
    18181 gccgctaaat cttctttagt gatcaagcca ccgtttttct gcatctcctg ggcgatctgt
    18241 tccgcaatcg tgcctttata gaattcgtcc gggccgtttt cagcaatcat ctccaggctc
    18301 tttgccaggt tcgcctgcac cagcgtgtcg ccctttttca gcggctcgcc ctctttccag
    18361 aagatagctt tactgttttc gtgattcggc aacacttcgc taccgtaggt tttgagatcg
    18421 tcagccagcg cgtcgttaac gataaaacca tcgcgtgcca gtttaaacgc gggctgcacg
    18481 actttgttca gcggcatggt gccgtattta tccagcgcca gcgagaaacc tgctaccgta
    18541 cccggtgtgc cggaagccag atgcgaagtg agtgattttt tgctgtccgg gttgccctga
    18601 tcatcgagga acatatcgcg ggtcgctttg gcgggtgcca tttcgcggaa atcgatagcc
    18661 gtggtattgc catttttcga gcggattaac ataaaaccac caccgcccag attccctgcc
    18721 tgcggatgcg ttaccgccag cgcgtagccc accgccacgg cggcatcaac ggcattcccg
    18781 ccctccttga ggatatccac ccccacctga gtggcagtgg cgtccacaga cgctaccatt
    18841 ccctgtttcg cgcgtaccgg atggaagaca tcttcctcca caccatacga gacgggcggc
    18901 gcaggaggcg cggcggcggc gctaaaacaa cttcctgaga gcagagcagc aatggccacc
    18961 cggcgtaaaa acgtcggttt tatcatcgtt attctccaga gattaagggg caaccccagc
    19021 taagcctggt atataactct gaattaatca tcgttttgtc gggaagcgag taaacttaaa
    19081 ggatatcttc agaggaggat acgcgatgaa acgacttctg attcttacgg cactcctgcc
    19141 gtttgtcggc tttgcacagc ccattaatac tctgaacaac cctaaccagc cggggtatca
    19201 gatacccagc cagcagcgga tgcaaaccca gatgcagact cagcaaatcc agcaaaaagg
    19261 gatgctgaat cagcaactga aaacgcaaac tcagttgcaa cagcagcatt tagaaaacca
    19321 gataaacaat aattctcagc gggtgttgca gtcacagccg ggggagcgaa atcccgcccg
    19381 gcagcaaatg ctgcccaaca ccaacggcgg gatgttaaac agcaaccgta atccggatag
    19441 ttcgttgaat cagcagcata tgctgccgga gaggagaaac ggcgacatgc tgaatcagcc
    19501 cagcacgccg cagcctgaca ttccgttgaa aactattggg ccgtaaagtt cggaccaatc
    19561 acgtcaatcg catcggtaca gatgcaatcc acgccccagc gcagcaactc tgccgcgtgc
    19621 tggggtttat tgacggtata aaccagaatc cgtaatccgg cgtctttcaa ctgcatcact
    19681 cgcgctttat cgagtaactt atgattgaga tgaatagaga cgcagcccag ccgcgcggtc
    19741 agttcgcgcc agtcgtcgcg ccactcatca agcaataaac cacgcggcag ttccggtgcc
    19801 gcctgttgtg cagcttctaa agcatcaatc tcaaacgatg acagcagcgg cggcgtcata
    19861 ccggcccaca gttggcgtgc cgccagcgcc accattttgc ccgttaatgg tccagtgcca
    19921 gtggtgggtt tgatttcgat attcgccatc atcccgtgtt cgcgacagcg ttccgccacc
    19981 tgcgaaagca acggcagcgg ctcaccttta aacgctttgc tgtaccaact gcccgcatcc
    20041 acgcgcagta aatcctgcca gttcagttca cccgcgacgc cccagccgtt gctggtgcgt
    20101 tcgagattgt cgtcatggag caggaagatc tcgccatctt tcgataactt cgcgtcaaat
    20161 tcgatcatct tatgaccgta ttttgccccg acgtcgattg ccgccagggt gttttccggg
    20221 gccagcttac cgccgccacg atgagcgacg atgcggggat aaggccagtt actcatactc
    20281 gttgtcctgt ttcaccatca aaaagatgca gctgattttc cgccagatgc agccacagcg
    20341 tgctgcctgc cgtcgggcgc tcctgatgcg ccagtcgcac caccagcttc tgcttgcgag
    20401 cttagcgcaa tatgttccgg gcgaatgccg agggtcattt tacgcccggc atactgacgg
    20461 tagccaccgt ttagcggtag cacgataccg ccgtccagtt cgaaatgcgt gccttcatta
    20521 ttcatgcggc ctgtcagcag gttcatcgcc gggctgccga taaaactcgc cacaaacagg
    20581 ctggcgggct tttcgtagac ttccaccggc gtgccaatct gttcggcaac gccgccgttc
    20641 atcaccatta ctcgctgggc gagcgtcatc gcctcaacct gatcgtgagt aacgtagagt
    20701 gaagtcgttt tcaggcgacg gtgcagctgt tgcagttcaa gacgcatctg tacgcgcagc
    20761 ttggcatcga ggttagagag tggctcatca aacaagaaca ccgccggatc gcgcacaatc
    20821 gcgcgtccca tcgccacacg ctggcgctga ccgccggaaa gctcgcgcgg gcgacgtttg
    20881 agcagaccgt ccagctccag aatgcgcgcc gcttctttaa cgcgctcggc tatttgctgc
    20941 ttgcccatgc cgcgaatttt cagcccccac gccatgtttt cttcgacgct catatgcgga
    21001 taaagcgcgt aattctggaa caccatcgca atcccgcgat ctttcggctc catttcggtc
    21061 acgcgctggt cgttgatcca gatatcgcct tcggtcaccc tctccagccc ggcaaccata
    21121 cgcagcagcg tcgatttccc gcagccagac ggcccgacca tcacgataaa ttcgccatcc
    21181 gccacatcaa gggtcagcgg tttaatgacc taggttttgc catcccagct tttggttact
    21241 gcctgtaatt tcagtcctgc catcttattt ctcactatcg accaggccgc gcacgaaggc
    21301 acgctgcatc actaaaacaa tcactactgg tgggatcagg gttaacaaca tcgccgccat
    21361 cactgagttc cattccgtgg tgccttcgcc agtggcgatc atccctttga tccctgccac
    21421 ggtggtgccg agatccacgt cggtaataat caacaacggc cacaaatact ggttccagcc
    21481 gtaaataaag gtgatcacaa acagcgccgc cagattagtt ttggagagcg gaaaaacgat
    21541 gtcgcaaaag aagcgcattg gtgacgcacc gtcgatccgc gccgcttcca ccagttcatc
    21601 cggcagcgtc ataaagaact ggcgaaacag aaacgtggcg gtcgccgagg ccatcagcgg
    21661 cagcgttaaa ccggcgtagc tgtcgagcat cttcaggttg gcgatgactt ccaccgtcgg
    21721 gaagatacgt acttcaaccg gcagcatcag ggtgataaaa atcatccaga agaagaggtt
    21781 acgtagcgga aaacgaaacc agacaatggc aaatgccgag agcatcgaga cagtaatttt
    21841 gccgagcgta atgctgaacg ccatcacaaa gctgttaagc aacatccgcc agaacggcgc
    21901 gctatttgtg cctaccccgt tcacccagat gttgtggatg ttttccagca gatgcgtgcc
    21961 ggggatgagc gtcatcggcg cggcgtagac ggcctgttta tccagcgtcg ccgcgacaaa
    22021 cgccacgtac agcgggaaga ggatcaccgc gatcccgagg atcagcatgg tatggctgaa
    22081 tatcgtcagc cacggacggt tctcaatcat tggtaacgca ccttgctttc aacatagcgg
    22141 aactgcacca ccgtcagcac gatgacgagg aacatcagca ccatcgactg tgcggcagac
    22201 gaagccagat ccagtccggt aaaaccttcg cggtagatct tataaatcag cgtcgtggtg
    22261 gcctgaaccg gcccgccgga cgtggcggcg tcgatcaccg ggaaggtgtc gaagaaggca
    22321 tacaccaggt tcactaccag caggaagaaa ctcaccgggg cgataagcgg cagcgcaatc
    22381 ttaaagaagc ggcgaatcgg ccctgcaccg tcgatggctg cggcttcgat caacgaacgg
    22441 ggaatggatt gcagcgcggc atagaagaac agaaagttgt agctgatttg cttccacacc
    22501 gaggcaaaca ccaccagaaa catcgcctga ccgctatttt gcgcgtggtt ccagtcgtag
    22561 ccgaactcag cgagaaaatg ggtgatcagc ccgcgaccgg ggttaaacag gaagatccac
    22621 aatacggcgg caacggcggg agccacggca tacggcagca acattaaggt ttggtagaaa
    22681 cgactgccgc gcacgatgta ctccaccagc gccgcaaaga acagcgatac cagcaaaccg
    22741 ctgacggtga caaaggtgct gaattttatc gtcgtccaga aggagtcgag ataatagctg
    22801 tcatgaaaca gcgtgacgaa gttatccaga ccgacaaact ggctggagaa accaaacgga
    22861 tcgacgcttt gtagcgagta ccacaacgct tcgcccgcag gccagataaa aaagataacg
    22921 gtgatgatga gctgcggcgc gaccagcaga taaggcagcc agcgcgagcg gaacaccgga
    22981 cgggatgatg acattacggt taattcctga actgtgccgg atgcgcttcg cttatccggc
    23041 ctacacgcga tgccaggtca gataagcgaa acgcatccgg cattacactg attaagactt
    23101 cgtcgatttc tcaaagcggc gcagtaactg gttcccacgc tcaactgcgg tatccagcgc
    23161 ctgctgtggc gtcttcttac cggtccacac gctctcaagc tcttcatcca caatcacgcg
    23221 gatctgcggc atgttgccca gacgaagccc tttggtgaac ggcaacggcg gcttgttcag
    23281 catctgacgc gtcgcggtat ccgcacctgg gtttttctcg taaaagccct gctcacgggt
    23341 caggtcatac gccgctttgg taattggcag ataaccggtt ttctgatgcc actcagcagc
    23401 gttttctggc ttcgcgagga agtcgagaaa cttcgccacg ccggtatacg tttctttatc
    23461 tttgccctgc atcacccaca ggctggctcc gccgataatg gcgttttgtg gcgcatcttt
    23521 cgcatcggcg tcgtaaggca tcatacctac gccgtagtta aatttggcgt actcgcgaat
    23581 gttggcaaga gaaccggaag aggcggtggt catcgcgcaa tcaccgttat agaacttctc
    23641 ggtggattca tccttacgac cgacgtagct gaagtcgccc ttcttgttca tctcctcgag
    23701 catggcgatg tgtttcacct gctccggctt gttgaactcc agcaccgcgt ccgtgccgtc
    23761 aaagccgttg tttttgctgg caaacggcag accgttccag gcgctaaagt tttccagttg
    23821 gatccagccc tgccagccgc tggcgtagcc gcacttaata ccggaggctt tcagttttgc
    23881 ggaatagtcc gccagatcct gccaggtttt cggcggctgt tccgggtcta accccgcttt
    23941 cttgaaggcg tctttgttgt aatagagaac ggaagtcgag ctgttgaacg gctgggagag
    24001 taagtggccg gttttgctgt cggagtagta accggaaacc gtcggcacaa actgcgactc
    24061 atcgaactga atccctgcct ctttaaacac gtcatacacc ggtttaatgg ctttcgacgc
    24121 catcatggtg gcggtgccaa cttcataaac ctgcaaaata gccggagcgt tgccggtacg
    24181 aaatacggca atccccgcgc ttaaattctg ttcgtagttg cctttatagg tcggtacaat
    24241 tttgtaatcc ggattttcgg cgttaaaacg ttgggccaga gaatccacct ctttacccag
    24301 ttccccttcc atagaatgcc agaacggaat ggtcgtcact gcctgtgcat tccccattaa
    24361 cgccagtccg agcgccagtg ctgaagctgt ataatgtaac ggtttcatcg tttatctctc
    24421 ttgttgtacc gaatgcgcga attcacgcgt tttatgctcg cggggtaaca tgacatgctc
    24481 gaattacaga aaaataactt ttttgttaca tttgtaagat agtaaggtgt cagaaagatg
    24541 acaaggcggt gacggcgtgg gtgagggaaa atgggagatg gggcacggat aagcggaaaa
    24601 atagaaggtc tgaatcaaac tctacagatt actcatcgtt tcatgccgga tgcggcgtaa
    24661 acgccttatc cggcctacaa gatcgtgcaa attcaatata ttgcaactca cccagtaggc
    24721 ctgataagcg cagcgcatca ggcaattttg catttgtcac ctgtctcaaa ggagtctttt
    24781 gactccttat caatcaacgt gttattaccc gcctaaatac gcacttctca ctgcttcgtt
    24841 cgccagcagc gcgtcgccgg tatcggagag caccacatgg ccgttttcca gcacgtagcc
    24901 gcgatccgcc agctttagcg cctggttggc gttctgctcg acgaggaaga tagtcatccc
    24961 ctgctcgcgc agctgctcga tggtgtcgaa aatttgctgg atgataatcg gcgcaagacc
    25021 gagcgatggc tcatcaagta acaacaaacg cggattgctc atcagcgcgc gaccaatcgc
    25081 cagcatctgc tgttcaccgc cggacatggt gcccgcccgc tgaacacggc gctcatgcag
    25141 acgtggaaac agttcataca cccactttat gcgctcctga aactggtcac gttcggcaaa
    25201 gaagccgccc atcgccaggt tctcttccac cgtcatccgc gagaagacgc gacgcccttc
    25261 cgggacaatc gctaccgctt cgcgcatgat tttcgccgtc tgccagtcgg taatgtcttt
    25321 atcatcaaac acaattcgcc cgctggtggc gcgcggatcg ccgcataacg tgccgagcaa
    25381 ggtggttttc cccgccccgt tcgcgccaat gagagtgaca atctcgccct gattgatatg
    25441 caggctcacc tcatgcagcg cctggatttt gccgtagtgg gcgctgactt tgtcaaagga
    25501 caacatgact ttttccatct tatgcctcac ctaaataggc acggatcacg tccgggttat
    25561 tacggatttg ctccggcgta ccgtttgcca gtggcgtccc ctgattgacc acataaattc
    25621 ggtctgaaat ccccatcacc agcttcatat cgtgttcaat caacaaaata gtggtgttgt
    25681 gatgattgcg cagttcggca atcagctcat ccagctcttt ggtttctttt gggttaagac
    25741 ctgccgccgg ttcgtcgagc attaaaatct ccggctgcgt caccatgcag cgggcaatct
    25801 ccagacggcg ttggtcacca taggccaggt tactcgcctg acggttggcg tgttccagca
    25861 aaccaatgcg ctcaagccag gtggcggcgc ggtcgagcgc ttcgctctgg gcgcgacgga
    25921 aagaaggcgt tttcaacagg ccagagaaca gcccggtttt cagttgctga tgctgcgcca
    25981 ccagcaggtt ttcaattacc gtcatttcac ggaacagacg cacatgctgg aaggtgcgca
    26041 ccacgcccat gcgagcaatt tgctgccccg gtaaaccttc aagatgctga tcgcgcagta
    26101 aaatggtgcc gccggtgggt ttgtagaaac cggtcaggca gttaaaaacc gtggtttttc
    26161 cggcaccgtt agggccgatt aacgagacga tttcctgcgg gtacagttca agattgacgt
    26221 tgttcaccgc cagcaggccg ccgaagcgca tcatcagacc gttaacagat aataatggct
    26281 gactcatgcc tgctctcctt tcgctgcgcc gtttttcagc ttcagttgcg ggcgcgtcat
    26341 gggcagcaaa ccctgcggac gccagatcat catcagcacc atcaaaccac cgagcattaa
    26401 catgctgtat tcgttgaaat cacgcatcaa ttcgcgcgac accaccagca gaacagccgc
    26461 cagaatcact gcaaattgcg agcccatacc gccgagcacc actatcgcca gcacaaaggc
    26521 cgattcagca aaggtgaagg attccgggct gacaaagccc tgacgcgccg caaacagcgt
    26581 tccggcaaaa ccggcaaatg cggcgctgat ggtaaaagca gtcagcttga tacggcgcgg
    26641 gcttaagccc agcgaacgac aggcgatctc gtcttcacgc aacgcttccc acgcgcggcc
    26701 cagcggcatc cgcagcaggc ggttaatgac aaacaggctt aacaccacca gcagcaacgc
    26761 caccaggtag aggaagataa cgcgatcgga cggatcgtat ttcaggccaa agaaattact
    26821 gaacgtgtcc cagccgcctt cacgagcggt acggctgaac tcgagtccga agagtgtcgg
    26881 tttcgggatc tgactgattc cgttcgggcc gccggtgatt tcagtgttat tgagcagcaa
    26941 tatgcgcaca atttcgccga aaccaagggt aacgatcgcc agatagtcac cgcgcagacg
    27001 cagcaccgga aaaccgagca ggaaacccgc cgccgctgcc atcagtccgg caatcggcaa
    27061 acaggtccag aaccccagac cgtaatagtg attgagcagc gcaaaagtgt aagcgccgat
    27121 ggcgtaaaaa ccgccgtacc ccagcaccag cagaccagaa agaccaacaa ccacattcag
    27181 cccgagaccg aggataatgt agatcatggt cagagtggcg atatccaccg tcccacgtga
    27241 caccataaac ggccacgcca ccacaagcac cagcagcgcc acgaggaaca gcttttgctt
    27301 caccgtggac ccatcaatcg cgggcagaat aaacttcggc ccggaaacgc ttttcaaccc
    27361 tttctggaaa gccggtcgca aaagctggaa gaaaaagacc accgccgtgc cgataaacat
    27421 ccactgccaa cggatgtctg aagccgtgtc gaccaccagt ttggtgccat ccagctccag
    27481 ttgtacgccc ataaagacgc ccgccaggac aaagaacatc gcggcagaga gcagtgccat
    27541 tgcaatatgc atcggtttca tactttctct acctccgggc gacccagaat accggtcggc
    27601 atcaccagca gcaccagaat cagcagggcg aatgagacca catctttata ttccgtactc
    27661 agataggcag aagagagcgc ctccgcaatc cccaggatca gcccgccaat catcgcccct
    27721 ggaatgctgc caatcccacc gagcaccgca gcggtaaagg ctttcatccc ggccataaag
    27781 ccgatatagg ggttaatgac gcagtagaac tgaccgagca gtacgccagc cactgccgcc
    27841 atcgccgcgc caataacaaa ggtcaaagca atcacccggt cggtgttgat gccaagcagg
    27901 ctcgccattt tcagatcttc cgcgcaggca cgacaggcgc gccccatacg ggaatagcga
    27961 atgaaaatcg tcagcgccag catggcgagg aaggtgacaa tccagatcac cgcctgcatg
    28021 gtggtaatag aggcagagaa gttttcgcta tggcccacca cccactgacc gttaaacagg
    28081 ctcggcagcg ccacgtcgcg cgaaccttcg gtcaggctga cgtagttttg caggaagatg
    28141 gacataccga ttgcggaaat aagcgcaatc aggcgcttag agttacgcac cgggcggtaa
    28201 gctacccgtt cgatactcca gccgtaggcg ctggcgatga cgattgcgcc gacgaatccc
    28261 gccgccacca gcagccagcc ggtatcaatg cccatcatca tcagcgcggc gatgatcata
    28321 aatgagacgt agctgccaat catataaacc tcgccgtggg cgaagttgat catgccgata
    28381 atgccgtaaa ccatggtgta gccgatggct atcagcgcgt aggtactgcc cagcgtgacg
    28441 ccgttaaaca tctgctgcaa gaaatacaaa aactgctcag acataaggta acctttctaa
    28501 acccgcccgc attttacggg cggtgggatg atcacttggc tgccgtggat gaaccgtcgg
    28561 cgtgccactg gaagacacca aaatcaaatc ccttaagatc gcctttttca tcccagttca
    28621 gcggcccaat cacggtgttt gcaccgttag cttttaaatc tttcaccagc gccagcggct
    28681 catcgctgcc ggtacgctca agggcagttg ccagagattg cactgccgcg taagtgatcc
    28741 agacataagg cccggacgga tctttcttgt ctgctttcag cgcatcaacg atgccctggt
    28801 ttgccggatc ctggtcatag cgttttggca tagtgaccaa catgccttcg gcggcatcac
    28861 cggcaatgtt cgacagcgat gcgttaccga caccttccgg ccccataaac tgggttttca
    28921 ggccaacgga acgggcttgg cgcagcatct gccccatttc cgggtagtaa ccgccgtagt
    28981 aaacgaagtc gatattttct tttttcaggc gggcgatcag cgcggagaaa tctttctccc
    29041 cggcggtaat accgtcgaag aagacgacgt tggcgttagc cgctttcagc ccgtcctgca
    29101 ccgaacgcgc cagcccttcg ccatactgtt gtttgtcgtg aatgatggcg atgcgctggg
    29161 gcttcaccgt ctcaagaatg tattttgccg ccgtaggccc ctgggaagag tccagcccgg
    29221 cagtacgcat aatgtgttga taaccgcgtt gggtcagctc cgggttggtc gctcccggcg
    29281 agatcatcag aataccttcg tcttcgtaga tatccgacgc tggctgggta gaggaagaac
    29341 acagatgacc aataacgtat ttaatgccgt cgttaacgat tttgttggcg accgcaacgg
    29401 cttgtttcgg gtcgcaggcg tcgtcatatt ccacgccaac cagcttgtcg cccttaattc
    29461 cccctttgac attaatgtct ttaatcgcct gacgcgcgcc gttaaattcc atatcgcccc
    29521 actgggcaat cgggccggac atcgcgccga caacggcgac tttaatatcg tcagccatag
    29581 cggtgtgtga aattgccagt gcaatcatcc ctgcgatgat agttttcgca ttccgtttca
    29641 tagtcaaaaa tccccattcg tgatgttgtg ttgctttgtt tttatgtgtt aacaaatcag
    29701 actgttcttt ttttatactg cactgttttt gcctgtctga ttaaggggtt agcgcagtat
    29761 tttgtgatga tagcgattaa aatccctatt tttcagtcga ttaagaacag ataatattct
    29821 gaatttattg atagataaac agaaaaaagt gcctttgtca gcataaaata acggcacaaa
    29881 gggcggaata attcactatc attcagggga ttatgctgga catttttcat tctctaatgt
    29941 tttaactttg taattattgc tgttaaaaaa ttaatcacct gccaaaagaa ataaaaaaga
    30001 gaaagcctcc gattaaatta tttcgctaca ctggttccac ttttgtgatt tacacgggtt
    30061 acccatgaag ctgaccatca ttcgattaga aaactttagc gaccaggacc ggattgacct
    30121 gcaaaagatc tggccggagt attccccttc ctctttacag gttgacgata accaccgtat
    30181 ctacgccgcg cgttttaacg agcgtctgct tgcggccgtg cgggtaacct taagcggcac
    30241 cgagggagca ctggattctc tacgcgtgcg ggaagtcacc cgccgtcgcg gtgtggggca
    30301 atatctgctg gaagaggttt tgcgtaacaa tcctggcgtt tcatgctggt ggatggcgga
    30361 tgcaggcgtg gaagatcgcg gtgtgatgac ggcgtttatg caggcactgg gatttacggc
    30421 acagcagggc ggctgggaga agcgttaatc gtcaagttga tttcaaaagt gatgaccaac
    30481 gagaaattgc ctgatgcgct acgcttatca ggcctataat gtgtgctgca atttactgat
    30541 ttctttggtt cttgtaggcc ggataaggcg tttacgccgc atccggcatg aagcaacgta
    30601 ttcgatatta gcaatttggc ggcaacccaa agttgccgat taacaattac ttcgcatcgg
    30661 tcgccgtgcc gttggcgtgc cagtcaaata cgccgaactc aaagcccttt agatcgcctt
    30721 tctcatccca ggtcagtggc cccatcacgg tatccacgga gttcgctttt aggtatttgg
    30781 cgatttcagc cggatcgtca gactgattca ggcccgcctg caaagattgc agcgcggcgt
    30841 aggtggtcca gacgaatgcg ccgcttgggt cctgtttttt cgctttgatc gcatcaacaa
    30901 tgggtttgtt cgccggaacc tgatcgtagt tcttcggctt ggtcaccagc agcccttccg
    30961 ctgattcgcc cgcaatgtta gacagcgaaa cgttagccac accttccggc cccataaact
    31021 gagttttcag ccctgccgcg cgtgcctgac gcaggatttg ccccatttcc gggtgataac
    31081 cgccgtagta aacgaagtcg atattctctt ttttcagacg cgccaccagc gttgagaaat
    31141 ctttttcccc ggcggtgata ccatcaaaga acaccacgtt agcattgcct ttcttcaggc
    31201 cgtcctgcac cgctcgtgcc agaccttcgc cgtattgctg tttgtcgtga acgatagcaa
    31261 tacgctgcgg tttcactttc tcaagaatat atttcgccgc cgtcggcccc tggtcggagt
    31321 ccaggccggt ggtgcgcagg atcagctgat agccacgggc ggtcagctcc ggcgcggttg
    31381 ccgctggggt gatcattaaa atgccttcgt cttcgtagat gtcagacgca ggctgcgttg
    31441 atgaagaaca gaggtgacca atcacatatt taatgccgtc gttaacgact ttgttcgcca
    31501 ccgcaaccgc ctgtttcggg tcacaggcat cgtcatattt tacgatttgc agtttgttgc
    31561 ctttaatgcc gcctttagcg ttgatatccg caaccgcctg ctctgcgccg gtaaactcct
    31621 ggtcaccgta ctgcgcaacc ggaccggaca ttgcacccac gaccgcgact ttaatatctt
    31681 ctgccagagc catattgctg aatgccagcg cgatacatcc tgccagtaac gctttaccct
    31741 ttatgttcat cctgagaatc cccattcttc tggttattac gtgtgttgtg atgttgttgt
    31801 tcagcacttt atttcgtttt atgcatgact acccgtgctt tagcagcata ctctgctaaa
    31861 acatacccga tttttatgat attggaatag ctattttgac agtttattaa caatctgcgt
    31921 ggggattggc gttttgccgg agggggaatt gattattgta gaggcccaaa aaacaaaaac
    31981 cccggactct catccagggt tctctgctta atagcggaaa ttacgcttca atagcggcgc
    32041 gaagtttttt catcgcgttc ttttccagtt ggcgcacacg ttcagcggaa acaccgtaac
    32101 ggtcagccag ttcctgcaac gtggacttgt tgtcttcgtc cagccagcgc gcacggatga
    32161 tgtcctggct gcgttcgtcc agaccatgca tcgcgtcggt cagacggttt gccgcctgct
    32221 cttcccagtt atcatcttcg atgccgtcgg caaagttaga tgatttatcc tgcagataga
    32281 gcaccggtgc catcggctgg ctgtcggaat cgtcgtcgga agacaggtca aaggtcatgt
    32341 cctgtgccgc catacgtgat tccatctcac gtacgtcttt gctggttacg cccagttcac
    32401 gggccaccat ttcgacttca tcctggttaa accagcccag acgctgcttg gttttacgca
    32461 ggttgaagaa cagtttgcgc tgcgctttgg tggtcgcaac tttgacgata cgccagttac
    32521 gcagaacgta ttcgtggatc tctgctttga tccagtggac ggcgaaggag accaggcgca
    32581 cacccacttc cgggttaaaa cggcgcactg ctttcatcag gccgatgtta ccttcctgaa
    32641 tcaaatccgc ctgtggcagg ccatagcccg cataattacg agcaatatga acaacaaacc
    32701 gcaggtgaga caggatcagc gttttagctg cttccagatc gccatggtaa tgcagctttt
    32761 cagccagcgc ccgctcctcg tcagccgaca acatcggcca cgcgttagct gcccggatgt
    32821 aggaatccag gttgccaact ggggctaaag ctaaactttg cattttgtca gtcattcaaa
    32881 tcctctcaat cgatatcttc tggcgcttca gtggtagcaa caactgtgcc aaagcttaag
    32941 agcaacgagg ttatcattca ctgttttatc agaccgtgat tttatccaca agttcaatgc
    33001 aagcttgtga ataaattacg cacaaaatgt gacatagaga tgaaataccg ggaagagaca
    33061 acggggtctc tttccctgct acggaaccca ttgcagggaa agagtataac acgcttttat
    33121 tattcaggcg taaagtggcg taaatgttgt accgtggcaa gccacgctgc cacccagcca
    33181 atcatcgagc ataccagcag caatagcagg cattcatcga acgataaacc attgatatca
    33241 aacttcgttc cgaaaacctg tgccacttcc gccaccgctg acgacaaacg cagtaccaga
    33301 atttctgaca aaattaatga caacaatgcg ccagaaaatc ccagcagcgc accgccgtac
    33361 aggaacgggc gaaggatgaa accatccgtc gcaccaatca gtttctgcac gttgatggag
    33421 tcacggcgag caaagatgct cagacgtacg ctgttgccga tgacgaggaa cactgccgcc
    33481 accatcaaca cgccgatcat cgccgaaacg cgcccaacca gcccggtcaa cgccgccaga
    33541 cgggcaaacc agctgtcatc catccgcact tcgtcaatac cgttaatctg cgtgatacga
    33601 tcacgcagcg tattgagtga ttccgtcccc tgaaaatcga gtttcgggat caccaccgcc
    33661 actgccggaa gcgggttttc ttccagcata tccagcgcac caccaaaacc agaccagtta
    33721 cggaactcgc ccagtgcgtc ttcacgagaa agatagttca ctttctccac gccttgctcg
    33781 gcctgcaact gtgccaccac gcccgcagcg gcgtcatcgt ccagcgtttt ttgcaagtaa
    33841 acagtgattt gcggtgacgg ataatactgc gtcgccgcct gattaacgtt tttgtacacc
    33901 atataacaga cgctgggcag cgtcagggag atggcgataa ccatcaccgt taaaaacgtg
    33961 gcgaacggtt tgcttttcag atcctgcaat gcgccgtgga aggcatagcg cacctgttcg
    34021 ttgaaaacgt tggttttgcg atttaccggt tttggcgagg atttcgcgcg tttcggtgcg
    34081 ttacgaccgc cgtcgcctga gccgccgacc gatttacgga agcgatccag acgcccacca
    34141 aactgccgaa tatgattgat tgcatcgcgc ttattcatgg cccacgcctc catgcaagtg
    34201 accatcgctc agggtgagca tgcgatagga acgccgtgaa atcagattaa tgtcgtgcgt
    34261 tgccatcaat acggttaccc caacgcggtt aaactcttca aacagacgta gaatcccttc
    34321 cgacaacgcg tcgtccaggt taccggtcgg ttcgtccgcc agcagtaccg cgggcttatt
    34381 caccaccgcg cgggcaatgc caacacgctg ttgttcaccg ccggaaagct gaatagggaa
    34441 gttcttcgct ttgtccagta gcccgacttt atccagtgcc gccgacaccc ggcgacgaat
    34501 atcgtcaccg ctggcaccag cgataatcag cgggatcgcc acgttatcgt agacagtacg
    34561 gtccatcagt agatggtgat cctggaaaat catgccaatc tggcggcgca gaaacggaac
    34621 ttcacggttt ttcagacgcg tgatgtcatg gccgctaaac cagattttcc cggcgctggg
    34681 ccgctcaatc ccacagatca gcttcaggag ggtacttttc cctgcgccgg aatgaccggt
    34741 cagaaacgcc atctcacccg gctgcatatg gaacgtaacg ccctgcagcg cctgtctccc
    34801 accgagataa gccttgctga catgttcaaa gcgaatcatt gttaatcctc tcgggcaaaa
    34861 agtgcctcta taaagtcgtc cgccttaaac ggacgcaaat cctcaatacg ttcgccgaca
    34921 ccaatgtagc ggatagggat accaaactgg tcagccaccg agaaaattac cccgcctttc
    34981 gccgtgccgt ccagtttcgt tagcgtgatg ccggttaagc caacagcttc gtggaacagt
    35041 ttggcctggc ttaccgcgtt ctgcccggtg ctggcatcaa tagtcagcat aacttcatgc
    35101 ggcgcttcaa cgtcgagttt cttcatcacg cggacgattt tcttcaactc ttccatcaga
    35161 tgcgatttgt tctgcagacg tccggcggta tcggcaatca ggacgtcgat attacgcgct
    35221 ttagctgcct gaatggcgtc gaagataaca gaggcggaat ccgcaccggt atgctgggca
    35281 atcaccggaa tattgttgcg ctgaccccag acctgaagct gttcaaccgc agctgcacgg
    35341 aaagtatcgc ccgccgccag catcaccgat ttaccctgct gctcaaactg acgcgccagc
    35401 ttaccaatcg tcgtggtttt acccacaccg ttgacgccca ccatcaggat cacgaacggc
    35461 gttttatctt caacattcag cggctcatcg actttcgcca gaatctcgcc catctcttct
    35521 ttcagcaggc catagagcgc ctcggcatca cgaagctgct tgcgggatgc gccttccgtc
    35581 agattggtga taattttacg tgtggtttcc acgcccacat cggcgatcaa cagctgttct
    35641 tccagctctt caaacagatc atcgtcgatt tttttaccgc ggaacaggct gataaatccg
    35701 gaaccgagat tttctttggt ttttaacagg ctgcgtttca ggcgcgcgaa gaagccttct
    35761 ttggtcggtt tttcttgctc ctgggcaatt tctgcaaccg gctgctcttc ttctgccgga
    35821 ggaactacca tcaccgcctc ttctgccgct tcggcagcca gcgcctgtgc ttccagctct
    35881 tcgtcggtga tttcttcttt agccgcttct tcttctgccg cttcgacaat ctctacggtt
    35941 tccgcttcag cctgccactc ttctggcgaa acctcttcgg cgttgacgtc ttccggcaac
    36001 ggcagctctt cacgttcgat agccactggc tccggcgttt cttctacgac cggttccggc
    36061 tgtgcaacaa cttccgcttc aggctgcgct ttttcacttt caacaacctg ttcagtgact
    36121 tccacaacgt cggcagcaaa agtttccgcc tcggcttcag tatgcgcctg cggctgctct
    36181 tcaacggctt gttcagaagc cttcgcaggc tcttgcgcct gaacgatttc ttctacaacc
    36241 ggttgttcat tctgaacttc tgtctctttt tccggggtct gctctttttg accaaagccc
    36301 agccaggaaa aaaagccacg tcttttttct ttcgccattt gcgactacac tcctcgctgt
    36361 tgcttcatgg cacagcgtta acgctatgta catagcagct aaaaaaatga tgaaatagtc
    36421 tatcacttaa cttaattcac atcaccgcct gcaagtatgt gttatctggc ggattgagca
    36481 atttatcatg aaaaaaccga atcattccgg cagcggccaa atccgcatta ttggcgggca
    36541 gtggcgaggc cgtaaactcc cggttcctga tagcccaggt ctgcgcccca ccaccgaccg
    36601 cgtacgcgaa acgttgttta actggctggc tccggtcatt gttgacgccc aatgtctgga
    36661 ttgcttcgcc gggagcggcg cgctggggct ggaagcgtta tcgcgctacg ctgcgggggc
    36721 aacgttgatt gagatggatc gcgtggtttc tcagcagtta attaagaatc tggcgacact
    36781 aaaagcaggc aatgcacgcg tggtgaacag caacgcaatg tcattcctgg cgcaaaaagg
    36841 cacaccgcat aatatcgtgt ttgtcgatcc accgttccgc cgtggcttgt tagaagagac
    36901 gataaattta ctggaagata acggctggct ggctgacgaa gccctgattt atgtcgaaag
    36961 cgaagtcgaa aacggtctgc ccactgttcc ggcaaactgg tcattacatc gggaaaaagt
    37021 ggcgggtcag gtggcttatc ggctgtatca acacgaagca caaggagaaa gtgatgctga
    37081 ttaatattgg tcgtttgtta atgctctgcg tttggggatt tttaatcctc aatctggtgc
    37141 atcccttccc gcgcccgcta aatattttcg ttaacgtggc gctgattttt accgtgctga
    37201 tgcatggtat gcagctggcg ctattgaaat ccactttacc gaaagatggc ccgcagatga
    37261 ccaccgccga aaaggtgcgg attttccttt tcggcgtgtt tgaactgctg gtctggcaga
    37321 agaaatttaa agttaaaaaa taaccttatt gttcgccaac aaagctaaca aagcgcgttc
    37381 ctttatagct cagcgtacct ttatcgccca ctgtcagggc gtggtactgc tgggcgtcga
    37441 ggcgaaacgt ctgctccatt cctccgcttt gcggtttgaa gcttgcctca tagcgcatac
    37501 tggtgcctgc cggagtcact tcctgctggc gcgaacggcg atcgttaatc ggtttttccc
    37561 gcttgttgct caccaccacc agcttttgct ggagcggagc catatcatta tcagcttttt
    37621 cccgtcgctg ttgcataaaa cgaaacgatg cggcgacgac aattaagcca atgataacaa
    37681 taaagaaaag aggtggttta ctcatcttta tccctcatcg gaaaatgcgg aattaagcat
    37741 accctgccag ttatggtgtt gtcatccgtc caccctcgcc actaaactgg aagcaagacc
    37801 gtaggcattc tgcttacgaa aaaataacga attcaaggaa ctaagatgct ttggtcgttt
    37861 atcgctgtct gtctttccgc atggctatct gtggatgcat cgtatcgtgg gccaacctgg
    37921 caacgctggg tgtttaaacc gttaaccctt cttctcctgc tgttactggc ctggcaagca
    37981 ccgatgttcg acgccattag ctatctggtg ctggcgggac tgtgcgcctc actgctgggc
    38041 gatgcgctaa ccctgttgcc acgtcaacgt ctgatgtacg ccatcggcgc gtttttcctc
    38101 tcgcacctgc tgtacaccat ctatttcgcc agtcagatga cgctctcttt cttctggcct
    38161 ctgccactgg tgttgctggt tctgggtgcg ctgctactgg cgattatctg gacgcggctg
    38221 gaagagtacc gttggcctat ctgcacgttt atcggcatga cgctggtgat ggtgtggctg
    38281 gcaggtgaac tgtggttctt ccgtccgacc gctccggcgc tctctgcgtt tgttggcgct
    38341 tcgttgctgt ttatcagtaa ctttgtctgg ctggggagcc actatcgccg acgcttccgt
    38401 gcggataacg cgattgctgc ggcctgctac tttgccggtc acttcctcat cgtccgctca
    38461 ctgtatctct gataaaactt gactctggag tcgactccag agtgtatcct tcggttaatg
    38521 agaaaaaact taaccggagg atgccatgtc gacacctgac aatcacggca agaaagcccc
    38581 tcaatttgct gcgttcaaac cgctaaccac ggtacagaac gccaacgact gttgctgcga
    38641 cggcgcatgc tccagctcgc caacgctctc tgaaaacgtc tccggcaccc gctatagctg
    38701 gaaagtcagc ggcatggact gcgccgcctg tgcgcgcaag gtagaaaatg ccgtgcgcca
    38761 gcttgcaggc gtgaatcagg tgcaggtgtt gttcgccacc gaaaaactgg tggttgatgc
    38821 cgacaatgac atccgtgccc aggttgaatc tgcggtgcaa aaagcgggct attccctgcg
    38881 cgatgagcag gccgccgacg agccacaagc atcgcgcctg aaagagaatc tgccgctgat
    38941 tacgcttatc gtgatgatgg caatcagctg gggtctggag cagttcaatc atccgttcgg
    39001 gcaactggcg tttatcgcga ccacgctagt tgggctgtac ccgattgctc gtcaggcatt
    39061 acggctgatc aaatccggca gctacttcgc cattgaaacc ttaatgagcg tagccgctat
    39121 tggtgcactg tttattggcg caacggctga agctgcgatg gtgttgctgc tgtttttgat
    39181 tggtgaacga ctggaaggct gggccgccag ccgcgcgcgt cagggcgtta gcgcgttaat
    39241 ggcgctgaaa ccagaaaccg ccacgcgcct gcgtaacggt gagcgggaag aggtggcgat
    39301 taacagcctg cggcctggcg atgtgattga agtcgccgca ggtgggcgtt tgcctgccga
    39361 cggtaaactg ctctcaccgt ttgccagttt tgatgaaagc gccctaaccg gagaatccat
    39421 tccggtggag cgcgcgacgg gcgataaagt tcctgcagga gccaccagcg tagaccgtct
    39481 ggtaacgctg gaagtgctgt cagaaccggg tgccagcgcc attgaccgga ttctgaaact
    39541 gatcgaagaa gccgaagagc gccgcgcccc cattgagcgg tttatcgacc gtttcagccg
    39601 tatttatacg ccagcgatta tggccgtcgc cctgctggta acgctagtgc cgccgctgct
    39661 gtttgccgcc agctggcagg agtggattta taaaggtctg acgctgctgc tgattggttg
    39721 cccgtgtgcg ttagttatct caacgcctgc ggcgataacc tccgggctgg cagcagcagc
    39781 gcgtcgtggg gcgttaatta aaggcggtgc ggcgctggaa caactaggtc gtgttaccca
    39841 agtggcgttt gataaaaccg gtacgctgac cgtcggtaaa ccgcgcgtta ccgcgattca
    39901 tccggcaacg ggtattagtg aatctgaact gctgacactg gcggcggcgg tcgagcaagg
    39961 cgcgacgcat ccactggcgc aagccatcgt acgcgaagca caggttgctg aactcgccat
    40021 tcccaccgcc gaatcacagc gggcactggt cgggtctggc attgaagcgc aggttaatgg
    40081 tgagcgcgtg ttgatatgcg ctgccgggaa acatcccgct gatgcatttg ctggtttgat
    40141 taatgaactg gaaagcgccg ggcaaacggt tgtgctggta gtacgtaatg atgacgtgct
    40201 gggtatcatt gcattgcagg ataccctgcg cgccgacgct acaactgcca tcagtgaact
    40261 gaacgcgctg ggggttaaag gagtgatcct caccggcgat aatccacgcg cagcggcggc
    40321 aattgccggg gagctggggc tggagtttaa agcgggccta ttgccggaag ataaagtcaa
    40381 agcggtgacc aagctgaatc aacatgcgcc cctggcgatg gtcggtgatg gtattaacga
    40441 cgcgccagcg atgaaagctg ccgccatcgg gattgctatg ggtagcggca cagacgtggc
    40501 gctggaaacc gccgacgcag cattaaccca taaccacctg cgcggcctgg tgcaaatgat
    40561 tgaactggca cgcgccactc acgccaatat ccgccagaac atcaccattg cgctgggact
    40621 gaaagggatc ttcctcgtca ccacgctttt agggatgacc gggttgtggc tggcagtgct
    40681 ggcagatacg ggtgcgacgg tgctggtgac ggcgaatgcg ttaaggttgt tgcgcaggag
    40741 ataaggcaaa ccgatcgcaa cattgagcgc gatcggtccc ctcgcccctc tggggagagg
    40801 gttagggggt gaggaaaagg cggcatcgaa gccaatcagc ccctatcaac cgcctttacg
    40861 aatcaaataa cgataaggca gtccatccgt ctctttcgca accagttcgt gttccataaa
    40921 ggtacaaaac ccaggaatat cgcgggtagt ggccggatcg tcggcgataa tcagcaacgt
    40981 ttcgccaggc tgcatattgc gcacggtttt gcgcaccatc atcaccggtt ccgggcagcg
    41041 caggccaagc gcgtcgagtg tgtggtcagg gctggaaaag agatcggtca ttttcttctc
    41101 atcacttaaa aaaacggcgc tagtttacgc cctgtgagtc cgtaagcaaa ccaggttaac
    41161 gattgcgtga aaattaacca ttgcattgtc aacgtaaagc agtatcatgc ggcggctcga
    41221 aaaaagggta agcacgttat tatgttaagg taacagacgt gtcgtacgta ttgggttccc
    41281 tcaccccaat ggttaatcaa aaaggtacaa tatgaacgtt ttctcgcaaa ctcaacgcta
    41341 taaggcgttg ttctggttat cgttatttca tcttctggtg atcacctcca gtaactatct
    41401 ggttcagctt cccgtctcca tttttggttt ccataccacc tggggcgcgt ttagctttcc
    41461 gtttattttt cttgctaccg acctgaccgt gcgtattttt ggcgcaccgc tggcccgacg
    41521 cattatcttc gcggtaatga tccctgcgtt attaatctcc tacgtcatct cgtcgctatt
    41581 ctatatgggt tcctggcagg gattcggcgc actcgcccac ttcaacctgt ttgtcgcccg
    41641 tatcgccacc gccagtttca tggcctacgc gctggggcaa atcctcgacg tgcacgtttt
    41701 taaccgcctg cgtcagagtc gccgctggtg gctggcaccg acagcgtcca cactgttcgg
    41761 taacgtcagc gacacgctgg cctttttctt cattgccttc tggcgtagcc cggatgcctt
    41821 tatggctgaa cactggatgg aaatcgcgct ggtcgattac tgtttcaaag tgttaatcag
    41881 tatcgttttc ttcctgccaa tgtatggcgt attactcaat atgctgttga aaagactggc
    41941 agataaatcc gaaatcaacg ctttgcaggc gagttaaagg ttcgttatca gagttgtgat
    42001 aagatggatg aatgagccgt tatggccgtt tatcgaaagg aagaagtcaa tgcgcaatct
    42061 ggttaaatat gtcggaattg gcctgctggt tatggggctt gcggcctgtg atgataaaga
    42121 cactaacgct acggcgcagg gttcggttgc ggaaagtaac gctaccggga atcccgtcaa
    42181 cctgcttgat ggcaagttaa gtttctcgct gccagcggat atgaccgacc agagcggtaa
    42241 gctgggaacg caggccaata acatgcatgt ctggtccgac gccaccgggc agaaagcagt
    42301 catcgtcatc atgggcgatg atccgaaaga agatctggcg gtgttggcga agcgtctgga
    42361 agatcagcaa cgtagccgcg atccgcagct gcaagtggta accaataaag ccattgagct
    42421 gaaaggtcac aaaatgcagc agttagacag tattatctcc gcgaaaggcc agacggcgta
    42481 ctcttccgtt attctgggta acgtgggtaa tcaactgctg accatgcaaa ttacgctgcc
    42541 agctgacgat cagcaaaaag cgcaaaccac cgcagaaaac atcattaata cgctggttat
    42601 tcagtaagtt ttaagatgat gaggcggcct cagggacgtg ttccggaggc cgttttttta
    42661 atcgccacgt cagtaataac gcgattgcga ccagtcccgc cgccgccaga taaatcaccg
    42721 gtacgcccgc ccagctcatc accagcccag ccagtggtcc agtcacgcca agcgataaat
    42781 ccataaatac ggtgtaagtt gccagcgccg ccccctgatt ttgctgcgga accgctttta
    42841 ccgccactac acccaatgcc gggaacacca gcgaaaaccc ggcccccgcc agtaagacgc
    42901 cgattttcgc catccacggc atagtcgcca cgccaaccag tagcaggccg attatctcaa
    42961 cgctaaagca aatcatcgct acgtttaagc caccgatacg gttaatgccg ttagggaata
    43021 acaaacgcgt accgacaaac gcacagctaa acagcgtcag cgcgaaagcc gcaccgtccc
    43081 aacctttagc gtcataaaac agcgtgataa aggtggcgat gacgccaaat ccggcggaag
    43141 ccagtgccag cgccataccg tacagccaga cgcgcccaag caccgcgcga aacggcagcg
    43201 gtttgccttt actggctttt accgtcggac gcgggatcgc caacaaaatg gccaccagcg
    43261 ccacgcccat aatgattaac gctaacgcct gcaagccgcc ccagtgataa aacacgacgc
    43321 ctaacggcgc acccatcgcc atcgccccgt aagtgacaat gccgttccac gaaatcaccc
    43381 gcccgatatg cagcgagcca accactccaa cgccccatag ggtcgatccc gttccggcaa
    43441 aactttgccc aatcccaagg atgacgcgcc ccaggcaaag taataacagg ctgatgacag
    43501 gcagactggc ggttaatcct gccgtcagat accccagacc gctcaaaaag cagccgcata
    43561 aaccgaagac gacaatcttt ttgggtccca gcaaatcggc gtaacgtccg gcatgagggc
    43621 ggctcagcaa ggtggcgaaa tattgcaggc tgataaccaa tcctgcccag aaggcgctaa
    43681 agcccatcac atcatggaca tagcccggta atacagcgag cggcaacccg atggtgaggt
    43741 agctggcgaa gttaaacatg acaatggaga caatgcgcaa attcaggcgc aatccgttta
    43801 gcgcgggttc ggctacgggt tcgggcatga ggatcaccac atttttacaa cagtgtttca
    43861 tttttaccat gtgctgacgt gaaaatcagc agtaagaatc agaatattgc tggcgtgact
    43921 cccgcgctac acttaacaca aaaagtcaca aggaagcccc aatggaaacc cctcaacccg
    43981 ataaaacggg catgcacatt ctgctcaagt tggcctcgct ggtagtgatc ctcgcgggca
    44041 ttcacgcagc ggcagatatc attgtgcagc tgttactggc gctgtttttt gccatcgtcc
    44101 tcaacccgct cgttacctgg tttattcgtc ggggagtaca acgccccgtt gccattacga
    44161 ttgtagtggt ggtgatgctg atcgcactaa ccgcgctggt cggcgtactg gcggcatcgt
    44221 ttaacgaatt tatctctatg ctgccgaagt ttaataagga gctgacgcgc aaacttttta
    44281 aattgcagga gatgttgcct tttcttaatt tgcatatgtc gccggagcga atgctgcagc
    44341 ggatggactc ggaaaaagtg gttaccttca ccacagcgct aatgaccggg ctttccgggg
    44401 caatggcgag cgtgcttttg ctggtgatga ccgtagtttt tatgctgttt gaagtgcgcc
    44461 acgtccctta caaaatgcgt tttgcgctga ataacccaca gattcacatc gcaggattac
    44521 accgcgcact taaaggcgtt tcgcattatc tggcgttaaa gacgctactc agtttatgga
    44581 caggtgtcat cgtctggctg gggctggcgc taatgggcgt acagtttgcg ctgatgtggg
    44641 cagtactggc gtttttgctc aactacgtgc ccaatatcgg cgcggtaatt tccgccgtac
    44701 cgccaatgat tcaggtgctg ctgtttaatg gtgtttacga atgtattctg gtcggcgcat
    44761 tgtttttagt ggtccatatg gtcatcggca atattttaga accacggatg atgggccatc
    44821 gcctggggat gtccaccatg gtggtatttc tttcattgtt aatctgggga tggctgctcg
    44881 gcccggtagg gatgctactt tcggtgccat taaccagcgt gtgtaaaatc tggatggaaa
    44941 cgacgaaagg tggcagcaaa ctggcgattt tactggggcc gggcagaccg aaaagtcggt
    45001 taccgggatg aggcgacaag tgatacgata cgcactttca ttttccatta aacgttggcc
    45061 ctgatatgta tcggatagtt ctggggaaag tttcgacctt aagcgcagct ccactgccac
    45121 cgggtttacg cgagcaagca ccgcaaggtc cacgacgcga acgctggctg gcggggcgta
    45181 cattgctttc gcacacgctt tccccgctac cggagatcat ctatggcgaa caaggcaaac
    45241 ctgcattcgc gccggaaacg ccgctatggt tcaacttaag ccatagcggt gacgatatcg
    45301 ctctgctgtt gagcgatgaa ggcgaagtcg gctgcgatat cgaagtgatt cgcccgcgcg
    45361 tcaactggcg ctggctggcg aacgccgtgt tcagcctcgg ggaacacgct gaaatggacg
    45421 ccgtgcatcc tgatcagcaa ctggaaatgt tctggcgcat ctggacgcgc aaagaagcca
    45481 tcgttaaaca gcgtggcggc agcgcctggc aaatcgtcag cgtagatagc acctatcact
    45541 cctcgctgtc agtcagccat tgtcagctag aaaatttaag ccttgcgatc tgcaccccca
    45601 ccccctttac gctcaccgcc gacagtgtgc aatggatcga ttcagttaac tgatccgccc
    45661 acccgactgc ccatctattg atccagaaca ggtaatcagt atgacgaata cttaaaatcg
    45721 tcatacttat ttccgccatc tattttaatc cattggggtt accatgctct ccacactccg
    45781 ccgcactcta tttgcgctgc tggcttgtgc gtcttttatc gtccatgccg ctgcaccaga
    45841 tgaaatcacc accgcctggc cggtgaatgt cgggccacta aacccgcacc tttacacgcc
    45901 taaccagatg ttcgcccaga gcatggttta tgaaccattg gtgaaatatc aggcagacgg
    45961 ttcggtgatc ccgtggctgg caaaaagctg gactcattca gaagatggta aaacctggac
    46021 cttcaccctg cgtgatgacg tgaaattctc caacggtgaa ccgttcgatg ccgaggcggc
    46081 ggcagaaaac ttccgcgcag tgctcgataa ccgtcaacgt cacgcctggc tggagctggc
    46141 aaaccagatt gttgatgtta aagcactcaa taaaacagag ctgcaaatta ccctgaaaag
    46201 cgcctactat cctttcctgc aagaactggc cctgccccgt cctttccgtt ttatcgctcc
    46261 ctcgcagttt aaaaaccatg aaaccatgaa cggaattaaa gcgccgattg gcaccggacc
    46321 gtggattttg caggaatcga aactgaatca gtacgatgtc ttcgtccgta acgaaaacta
    46381 ctggggcgaa aagccagcga ttaaaaagat cacctttaac gtcatcccgg acccgactac
    46441 ccgcgcggtg gcgtttgaaa ctggcgatat cgacctgctg tacggaaacg aagggttatt
    46501 accgctcgat accttcgccc gctttagcca gaatccggct taccacaccc aactgtcaca
    46561 gccgatcgaa accgtgatgc tggcgctcaa taccgccaaa gcccccacca acgagctggc
    46621 agtacgtgaa gctcttaatt acgcggtaaa caaaaaatcg ctgattgata acgcgttata
    46681 tggcacccag caggtcgccg acaccctgtt tgccccttct gtgccttacg ctaatctcgg
    46741 cctgaaaccg cgccagtacg atccgcagaa agcgaaagag ttgctggaaa aagccggttg
    46801 gacgctgcct gcgggcaaag acatccgcga gaaaaatggt cagccgctgc gcattgaact
    46861 gtcgttcatc ggcaccgatg cgttaagcaa atcgatggcg gaaatcattc aggctgatat
    46921 gcgccagatt ggcgcagatg tctcgctgat tggcgaagaa gagagcagta tctatgctcg
    46981 tcagcgcgac ggtcgttttg gcatgatttt ccaccgcacc tggggcgcgc catacgatcc
    47041 acacgccttc ctgagttcaa tgcgcgtacc gtcgcacgct gacttccagg cacagcaagg
    47101 attagccgac aagccgctga ttgataaaga gatcggcgaa gtgctggcga cccatgacga
    47161 aacgcaacgt caggcgctgt atcgcgacat tctgacccgt ctgcatgacg aggcggttta
    47221 tctgcctatc agctacatct cgatgatggt ggtatcaaaa ccggagctgg gtaatatccc
    47281 ctacgcgccg atcgccaccg aaattccgtt cgaacagatt aaaccggtga aaccttaatg
    47341 ttgcgttacg tattacgccg ctttctgctg ctgatcccga tggtgctcgc cgcctcggtg
    47401 atcatttttc tgatgctgcg cctcggtacc ggcgacccgg cgctcgatta tctgcgttta
    47461 tctaacctgc cgccgacgcc ggagatgctg gcctctaccc gcaccatgct cgggctggat
    47521 cagccgctgt acgtccagta cggtacctgg ttgtggaagg cgctgcatct tgactttggt
    47581 atctcattcg ccagccaacg cccggtactg gacgatatgc tgaacttcct gcccgccacg
    47641 ctggaacttg caggtgcggc gctggtatta attctgctca cttccgtacc gctcggtatc
    47701 tgggcggcgc gccaccgcga tcgtttgccg gatttcgccg tgcgctttat cgcgtttctt
    47761 ggcgtgtcga tgcccaactt ctggctggca tttttgctag tgatggcgtt ttcggtgtat
    47821 ctgcaatggc tacccgcgat gggctacggc agctggcagc acatcatttt acctgcggtt
    47881 tccatcgcct ttatgtcgct ggcgattaac gcgcgactgc tgcgcgccag tatgctggac
    47941 gtcgccggtc agcgtcacgt cacctgggcg cggctacgcg gcctgaatga caaacagacc
    48001 gaacgccgcc atattctgcg caatgcctcg ctgccgatga tcaccgccgt ggggatgcac
    48061 attggcgaat tgattggcgg gacgatgatt atcgaaaaca tctttgcctg gcctggcgtc
    48121 gggcgctatg cggtgtcggc gatttttaac cgtgactatc cggtgattca gtgctttacg
    48181 ctgatgatgg tggtggtttt tgtggtctgt aatttgattg tcgatttgct caacgccgcg
    48241 ctggacccgc gcattcgtcg tcatgaagga gcgcacgcgt gaactttttc ctctcttccc
    48301 gctggtcggt acgcctggcg ctgatcatta tcgccctgct ggcgctgatt gcgctcacca
    48361 gccagtggtg gcagccgtac gatccacagg caattgattt gccgtcgtgc ctgctttcgc
    48421 cggatgcgca gcactggctg ggcaccgatc acttaggtcg cgatattttc tcgcggctga
    48481 tggcagcgac ccgcgtgtcg ctcggttcgg taatggcctg cctgctgctg gtgctgacat
    48541 tagggctggt tattggcggc agcgccgggt tgattggcgg gcacgttgat caggccacca
    48601 tgcgcgtcgc cgatatgttt atgaccttcc cgacctcgat tctgtcgttc tttatggttg
    48661 gcgtgctcgg caccgggctg accaacgtaa ttatcgccat cgccctgtcg cactgggcgt
    48721 ggtatgcacg catggtgcgc agcttggtga tttcactacg ccaacgcgag tttgtgctgg
    48781 cgtcacggct ttccggtgcg ggccatgtgc gggtgtttgt cgatcatctg gcaggcgcgg
    48841 tgatcccttc gctgctggtg ctggcaacgc tggatatcgg ccatatgatg ctgcacgtcg
    48901 cggggatgtc tttccttggc ctcggtgtga ccgcgccgac cgccgaatgg ggcgtgatga
    48961 ttaacgacgc gcgccagtat atctggaccc agccgctgca aatgttctgg ccggggctgg
    49021 cgctgtttat cagcgtgatg gcctttaacc tggtgggtga cgcactgcgc gatcatctgg
    49081 atcctcatct ggtgacggag cacgcacact aatgccgcaa cagattgaac tacgtaatat
    49141 cacgctacag gccgcgcagc cgctggtaca cggtgtatcg ttaaccctgc aacgcgggcg
    49201 cgtgctggcg ttagtcggcg gtagcggcag cgggaaatca ttaacctgcg ccgcgacgct
    49261 gggcattttg cccgctggcg ttcgccagac ggcgggggaa attttagccg atggcaaacc
    49321 ggtttcgcct tacgccctgc gcggcatcaa aattgccacc atcatgcaga acccgcgcag
    49381 cgcctttaat ccactgcaca ccatgcacac ccacgcgcgg gaaacctgcc tggcgttagg
    49441 gaaacccgcc gatgacgcta cgcttaccgc tgccatagaa gcggtggggc tggaaaacgc
    49501 cgcgcgcgtg ctgaagctgt acccgttcga gatgagcggc ggcatgttgc agcgcatgat
    49561 gattgcgatg gcggtgctgt gtgaatcacc gtttatcatc gccgatgaac cgaccaccga
    49621 cctcgacgtg gtagcacagg cgcgcatcct cgatctgctg gaaagcatta tgcaaaaaca
    49681 agcgccggga atgctgctgg tcacccatga tatgggcgtt gtggcgcgtc tggcggatga
    49741 cgtggcggtg atgtcacaag gtaaaattgt cgaacagggc gatgtagaaa cgctgtttaa
    49801 cgcccccaaa catacggtga cgcgcagcct ggtttccgct catctcgccc tctacggtat
    49861 ggagctggca tcatgacctt acttaacgtc tgcggccttt cccatcacta tgcccacggt
    49921 ggatttagcg gaaaacatca acatcaggcg gtgctgaata acgtttccct gaccctgaaa
    49981 agcggcgaaa ccgtcgccct gctgggacgc agcggctgcg gaaaaagcac cctcgcgcgg
    50041 ctgctggtag gcttagagtc tccctcacaa gggaatatta gctggcacgg cgaatcgctg
    50101 gcgaaactta atcgcgccca acgtaaagcc ttccgccgtg atattcagat ggtgtttcag
    50161 gactccatca gtgccgtgaa tccgcgcaaa accgtgcgcg agatcctgcg tgaaccgatg
    50221 cgccacctgc tatcactgaa aaaatccgaa caactggcgc gcgccagcga aatgctgaag
    50281 gcggtcgatc tcgatgacag cgttctcgac aaacgcccac cgcagttaag cggcggccag
    50341 ctccagctcg tctgcctggc acgcgcgctg gcggtcgaac cgaaattgct gattctggat
    50401 gaagccgttt ctaaccttga tctcgtgtta caggcgggcg tcattcgcct gctgaaaaag
    50461 ctacaacaac agtttggcac tgcctgcctg ttcatcactc atgacttacg cctggtggaa
    50521 cgcttttgcc agcgagtgat ggtgatggac aacggacaaa tcgccgaaac ccaggcggtg
    50581 ggagataaat taaccttttc ctctgacgcc ggacgtgtgc tacaaaacgc ggtattaccc
    50641 gcattccccg tgcgccgtcg cacctcagaa aaggtttaac gccaatgcaa cgagtcacca
    50701 tcacgcttga tgacgattta ctggagacgc tggacagcct gagccagcgt cgtggttata
    50761 acaaccgttc cgaagctatc cgcgatattc tgcgtagtgc cctggcgcag gaagctaccc
    50821 agcagcacgg cacacaaggt ttcgcggtgc tgtcgtatgt gtatgaacac gaaaaacgcg
    50881 acttagccag ccgtattgtt tccacccagc atcatcacca cgacctctcc gtcgccacgc
    50941 tacatgtgca catcaaccac gacgactgtc tggaaatcgc cgtgttgaaa ggtgacatgg
    51001 gtgacgtgca gcattttgcc gatgacgtta tcgcccagcg cggcgtgcgg cacgggcatt
    51061 tgcagtgcct gccgaaggaa gattgagtta tttctaaaac aaggaaagaa aagatattaa
    51121 ctgaacttaa tttataaaat taagttcagt tgcatagata gatcctgtaa aggcagatat
    51181 tctgcgtaat gtacgtcgct aaaatttata aatgattcaa acgctcattc agtgtttcaa
    51241 taatataggt attaacagag acttgttgct gcgcagccgc attattaagg cgttcactca
    51301 gcgattctgg atagcgtaac gtaaaggtct taatcttttc tgtacgcgca tacggctcaa
    51361 tacccgcagc tttacaatcc tcaagatact ctcgcaggga aagctctcct tctttttgca
    51421 gcccctgaat actgtcagag acaaaatcac aataacctga caagcccagg aatttgccac
    51481 gaaatgcatt cagttctggc acgtaggtaa taacagctgg ttgcccggca atctccatag
    51541 agtttggcgt cttcagtttg atcatggttt tacccctata cttagtaacc agtcacacac
    51601 gctttctaca gcgcctttat cggtatctgg cgacggatga ggccgatgga aacacgcaac
    51661 gctcatattt agtatgaact tgcaacgcga acctcttcct tccttaattt ctccgcctaa
    51721 tgctttaacc agtgactcta tatcagacca cttgatccct tgaggaacag gagttttaaa
    51781 tatttgatcc aatgtatttt tttgtttctt ccgtaaagcc tgtaccttgg ttctcatggc
    51841 atgtccttat gacttcactt tatgacatca cagtatgaag tcaagtttcc cttccttacg
    51901 ccattgtccc aatcgtcttc ctgaatcgca gcagtgcgat ggtgaaaaac gcgccgccaa
    51961 ttgccatcag cgtcaggaac tgcggccaga cgatttcgaa tccggcaccc cggtagagga
    52021 tggcctgcgc gaggctaaca aagtgtgtcg tcggcatggt cagcataatg tcctgcacca
    52081 tctgcggcat actttcgcgc ggcgtggatc caccggaaag catttgcagc ggcagcagca
    52141 ccagaatcac cagcagcccc agttgcggca ttgaacgcgc tatcgtcccc ataaaaatgc
    52201 cgattgacgt ggtggcaaac agactgagcg ccacgcccag cataaacagc gggatcgagc
    52261 cttcaatcgg tacgcccagt acacctttca ccatcagcac cagcgataat cccgatacca
    52321 ccagcaccac cagccccatc gaccagatct tcgccatcat gatctcaaac ggcgttatcg
    52381 gcatcaccag taaatgttcc accgtgccgt gttcacgctc gcggatcagc gccgatccgg
    52441 tcaatacaat cgccagcatg gtaatgttgt tgatgatcgc catcaccccg ccaaaccacg
    52501 cgggatcaag gttcgggtta aagcgcatcc gggtttccag cgataccaac ggttcgctgt
    52561 tatcacggta gcgcgcgaca aagctgttca cttcaccgtt gataatattc tggatatacc
    52621 cattgccggt aaatgcctgg ctcatgcgcg tggcatcgac gttcacctga atatccggct
    52681 ggcgtccggc gaggacatca cgctgaaaat taggcggaat atttatcgcg aaggtatagc
    52741 gtccggcgtc cagtccggca tccatctcat cggcggtgat catctccggt ggcaaaaacc
    52801 acggacgata gaagctgtta acgatccggt tcgataactg cgattgatcc atatcggcaa
    52861 tggcgatcgg cgcgaggttc aacgatcctg gcgtaacggt cgctgacgaa tacaccgaca
    52921 ccgtaaacga gaagacaatc agcgtcagca tcgctttatc accgagcaga ctgcgcaact
    52981 ctttgatacc cagattaaaa atattgcgta aatggcgcat catccctcct gttttttcag
    53041 cagcaggatg cttaagccca tcaccagcgg gatggctatc agcaacggga taaaaagttg
    53101 ccacaaatca gtcagatcca gcgctttcga gaacgttccg cgggcgatag tcagaaaatg
    53161 actggtcggg taaacctcgc cgatccaacg tccaggccct tccagcgaag ctaccggatc
    53221 gatcatcccg gaaaactgtg tcgccgggat caatgtgata atcgccgttc cgaaaatggc
    53281 ggcaatctgg cttttcataa aggtggagat cagcagcccc attccagtgg caatgatgat
    53341 atacagcagc gccgccaagg tgagcgtcag gaaactgcct ttatgcggta cgccaaacac
    53401 aaacaccgac aggccgcaga gcaggaaaaa gttcagcatc cccagcgcga tgtacggcag
    53461 ctgtttgcca agcaaaaatt cgctacgggt ggtgggggtc acgtaaaggt tgataatcga
    53521 ccctagctct ttttcccgca ccacgctaag ggcgcttagc attgacggga tcatcatcag
    53581 cagaagcggg atcaccgccg gaacaatcgc tggcaggctt tttacgtccg ggttatagcg
    53641 ataacgcgtc tcaatgttca tcagcccgct ttggctggcg ggtgtcgatt gtcggctcgc
    53701 cacatcctga agccaactct ggtgcattgc ctgcacgtaa ccttttaccg tttcggcacg
    53761 actgggcatc gctccgtcga tccagacgcc gagttccaca ggcgtaccac gcgcgatatc
    53821 gcgcccgaaa ttgggcggga tctcaatggc caccgtgata tcgcccgcac gcatccgacg
    53881 atcaagctcg tcataactgg tgagcggcgg ctgttcgata aagtaacggg aaccggagag
    53941 gttgagtgtc cacgcctggc tactgacggt ctggtcgcgg tcgagcaccg caaagcgcag
    54001 gttttccaca tccatactga tgccgtaacc cattatcagc atcaggatca ccgttcccat
    54061 cagcgccagc gtcgaacgta ctggatcgcg tcgcagttcc agcgcttcgc ggcggctgta
    54121 gctaaacaga cggcgcaggc taaatccctg acgcggcgcg tgggtggtgt cgtgtaccac
    54181 gggcggcgct tcggcttcgt tgctctgccc tgccgcttcc tgcaaatagg cgataaatgc
    54241 ctcttccaga ctggcggctc cgcgtttctc aaccagttcc tgcggtgtac cgctggcaag
    54301 cacttttccg gcgtgcatca gtgagatgcg gtcgcaacgt tccgcttcgt tcataaagtg
    54361 ggtggagatg aagatagtca ctttgtcctg gcgcgagaga tcgaccatca actgccagaa
    54421 catatccctc gccaccggat cgacaccaga agtaggctca tcgaggatta acatctccgg
    54481 gcgatgaatc accgccaccg ccagcgaaag ccgctggcga atgccgagcg gcaatgactc
    54541 cggcagaaca tcttcaacgt cgttgagctt aaaacgctcg ctcatttcag ccactcgcgc
    54601 gggaatttcc gcttccggga tgtgaaacaa acgggcatgt aactccaggt tttgccgcac
    54661 ggtgagttcg ttatagagcg aaaacgcctg cgacatatag cccacacgac ggcgggtatc
    54721 gatatctttt ggatcaaccg gttgcccaaa tagccacgcc tcaccttcgc tggcgggcag
    54781 cagtccggta agcattttca tggtggtgga tttaccgcag ccgttcgaac caaggaatcc
    54841 aaaaatctcc ccgcgtggaa tgcggaaatt gacgtgatca acggcaacga aggaaccaaa
    54901 acgcatggtc agatcgcgcg cttcgatggc aatctctgcg ttttcaggtt gatacggtgg
    54961 gatcactacc gcctgatgcg cctggcgttg cgcttgcggt aacagattaa taaatgcttc
    55021 ttccagcgtg gcgctttgcg tttgctgccg cagctcttcg gcgctgccgg ttgccagcac
    55081 ttctccggca ttcatcgcta ccagccagtc gaagcgttcg gcctcttcca tataggcggt
    55141 ggcgaccagc acgctcatat tgctctgccg ctggcgaata ctgtcgatca gatcccagaa
    55201 ctgggcgcgg gagagcgggt caacccccgt tgttggctca tcaaggatca acagttccgg
    55261 gtcgtggatt aacgcgcagc acagcccaag tttttgcttc atcccgccgg agagtttacc
    55321 tgccggacga tcgcgaaacg gtgctaaccc ggtgctggtc agcagctcat taattcgcgc
    55381 ttcccgctcc gctttatcgt gaccgaacag gcgagcgaaa aaatcgacat tttcatacac
    55441 cgacaaggtg tggtagaggt ttttgcccag cccctgcggc atccaggcga tgcgcgggca
    55501 gacgtctcgg cgatgcttcg ggtcgtgcat atcgccaccc agcaccacca cattgccctg
    55561 ctcaatgacg cgggcaccgg aaatcagtga caataaactc gacttcccga cgccatccgg
    55621 accaatcagc ccgaccatgc aacgggccgg aatatcgaga gtgatattgt tcagcgcaac
    55681 ggtttttcca taatgctggc tcacgcccgc cagttgcgcg acaggcggga cgggaaccag
    55741 ttccagatgc gtcattgcgg caacctcacc acgaggtcgt caggccacgg aagttcttca
    55801 ttcacccgca cccacgctac gcccggtaaa ccggttttga catattccag atgctgctgg
    55861 agtaattccg gtgggatacg cgctttgacg cggaacatca gtttcagccg ttcatcgctg
    55921 gtttcgacgg tttttggcgt gaactgggcg acactggcga caaaactgat ggttgcagga
    55981 atacgcagat ctggcgcggc atcgaggatc agccgggctt caccgcccag tttcagcgtg
    56041 cccgcctgtt cggttggcag gaagaaggtc atatagacgt cgctgagatc gaccatattc
    56101 agcacccgac cgcctgccgc cagcacttcc cctggctcgg caacccgata ctgcacgcgt
    56161 ccgtcacgcg gggctttcag ttcactgtca tcgatatctg cggcaatgcg ccgttcagtg
    56221 gcttgcgctg cttcgacccg agtttgcgcc tgaatgatat tggtgcgtgc tgcttctata
    56281 gccgctttag aagccgaaac ctgggctttc gctgattcca gcgcagctcg ggcgctctca
    56341 gcggcggcgc ggtcgtcatc cagctgttgc gcagaaatag cccctcgttg ggcgagcgag
    56401 cgggaacgcg tatgacgttt ggcaacagag tccagttcgg cctggcgttg attgaccagc
    56461 gactgtgcgg cacgagtttc actttgtcgt tgctccagca aagcctgcgc ggcggcaacg
    56521 gcgctttgtg cctctttgat ttgcgcgatg gcttccagtc gctgttcctg caacacgcga
    56581 gtatccatct tcgccagcac ttcgccttcg cgaacaaact ggccttcttt caccagaatg
    56641 gtgtcgatac gcccggcaat tttgctggcg atatccactt ccgtcgcttc aatgcgccca
    56701 ttgctgacag caaagccttc cggcacaccc gccgggcgca acagccacca cgccacgacg
    56761 gcagccaccg ccagtgcccc gacaacccac cacgccagat ggcgcttact cttatccata
    56821 atcgacccgc cataatccct gtaagcaaac gacgcgtgtt acaactgccg ccgaacgacg
    56881 ttcagcggat tcttccatcc gggataagaa ggaaacgaca aatacgacca gcgcgcagag
    56941 cattgagcgc caacggggaa ggcttagtga aaacgggtag taatgttgtc atcatcagca
    57001 ttcctggccg taaatgaaac acgcatactc ataagtgtga tctataaaac tggctgacaa
    57061 aacggtatcc atcctgttgg atcgcatttc acgcctgttc agttgcagga acagagcatt
    57121 cctttttacg ttttatcaac agtctcaacc agatagtaac attaatattc atgttgtcta
    57181 tggttcagag ctgtaacatt gtgcgccttc taatcaaagg cataagtcca tttctgtttt
    57241 tttcatgctg gtaagacttc cagggagtgg ttatctctgg ccctgcacca gggttaattt
    57301 ggcaattatg catgctgaac attcttccag catgtttact gtcacgggcg ttaagaccta
    57361 aaactgtccc cgtttcacct atgcgttctc tcccaccatt tccgttatgt gcaacaactt
    57421 gatttatacc taacatttgt gaacatgcta atagtgcttc aagtttaggt ctgtaatctg
    57481 tcgaaacaga aaaataacga ttaaaggatc gacaagagat agcccggcta gttttaaact
    57541 ttttgccatt aattatagca tgatgctttt tattaataat ttccgctaat tcgaccggat
    57601 cgagagcatt ggtcatttca tcaatagaga cagttatcgg tcctataatg taacgttttc
    57661 gctgatcatc aaaagttatc ccatgatgat ttgccattat tttgtatttg gggtcataga
    57721 aacatacagg ataatctttt atcccgtcgt aggtatcctc atcaagtggt ttcatgcgcg
    57781 tgaagttttg catatagtta ccattgcaat tcgtttcatg atttccagct aatacatgaa
    57841 cattttcatt gatactcatt atgcgtttta acaaatcgat gataaattga tcgccacaga
    57901 tattactaaa tcgatcgccg gtttggtcgc caagaaacgt acatggcgtg actgcgtctt
    57961 tattaataac caaataaggc tgaagacttt ctaaaacatt atctcttctg ttcagtatac
    58021 tgattattgt attaacatta gtgtcaagtt gctcattaaa gttctccctt agcaatacgt
    58081 tgagaacttc atcttctata ttaagcaact ggctaaggaa attttctcct tcagccatga
    58141 ttcccaaatg tcctgttgca aaaagcgcgt acaaaactgc accgactgag ccatcggtat
    58201 cgccaaaata ggtcggcctg gtacttgtgg gatcaacagg tgaacaattg gagaaatagg
    58261 ttctgtcagc gaactggggt tttataattg tttcgaattt aatatcaaat acattgtatt
    58321 gcgatatttc ccagttccag atgggtttgt acgactcagt aactgcaaca tcattagcat
    58381 tatcatttac tgtcatatta ttaagtaaat aaacaatgct gcaccaatta atatcggtat
    58441 tatcaagagg gcaaccgtaa tttttactat taattgtcac aataaactga taacagttat
    58501 tcctacgtag aatatgagcg cgcatgcccg atagcatact atatttttct gaatgattcg
    58561 tttgtatggt aaatgaaatt ttgtcatctt gaatagattg ttggaaacgt tctatttgcc
    58621 aatcatcaat tttttccaca acttcaccga gcattgtcct ggcaatactt gatatatcat
    58681 cacctatacc atttgcaata tacccaaaca gttctttagt gaagttggta acatgctctt
    58741 gcaccatatc atttgttgcg attgtcgttg tactgccatt agtgcctgcc acagttccaa
    58801 ttttcatctt ttacctcata ttattcaccg cgaggaatac atagttaaca ttttatctta
    58861 tgacatacac ctcatattcg atggggaaat aattatattt tgcacaaatc attcagcctt
    58921 aaagcataat atgaaatttc ttatctcata gcagagaaag tccctaaata ttgtagaggc
    58981 gtcatcagta catacctcta caatcgatta agaaaaccta tgaaatactg gcaaaattaa
    59041 aaagagatac cacagcactc atgaagtttt attgaggttg aaaacaacta agccctggga
    59101 cacctttttt atcaccacgt tcgttaggtg ctatcttcca agaatttagc cccatggtat
    59161 aattatcaat tttttgatgc tggtctaccc ctaatccata atggccaaat ttctgtttag
    59221 tttttttgag cgtctgtcca catttgaaga tatcttcagg ttttggtctg aaagcagtag
    59281 tgttattaaa ataattttca tatacaggtg tgggtgctat agcattgctt ctaattagat
    59341 ggagaagatg atcatccatg tgttgtttat gttttttatt acatatattt gctaattcct
    59401 caggatcaat tgggtctttc atctgattaa atggaacctg caaagcaccg agacaataac
    59461 attgattttc ttcatttctt attattccgt gatgactggt taaaacttta gtttctgagt
    59521 tataattaca aacatcgagc gttttaatta agttatacgt atcaccggca gagagtttat
    59581 aattttctag tcgtgcagta tagttaccat taaaattgat ttcatgattt ccagccagaa
    59641 cgatgacatt tttattaata cggctatctt tattaccctc catatttcgc atagaattta
    59701 gcaaagtaag gatatattta tcaccaaaaa tagtactaaa tctatcgcct gtatgatccc
    59761 caaggaagat acaaggggtt atcgcatcgt cctttaaaaa aacatgttga tgcaactcat
    59821 tcaatatcat gtctctcgta tttaataaat tgtagagcaa tttattattt ttatgtatgt
    59881 atgcagatga agccatgtct tcgtgtttta ataattcaca taataaactc caaccctttt
    59941 ccctgatgcc tatatgccct gaaacgagca atgaataaag tacagcgcct actcttccat
    60001 ctgtatcgcc aaaataagcc gtgttggcat tttgtgggtt aatcacacca cattgtttat
    60061 aaaagtctat tttagaaaac acagatttaa catagctatc aaacttatcc ttaggaaacg
    60121 tctctttaga gacatcccaa cgccagtcag ctttaaaatt accgctaaga agagtaaaga
    60181 aattttcagg tatgatagaa agcccattaa ctttataaat tatgtctgat ttatctccgg
    60241 agaaattaat aaaataataa ccatccttac actcaatctt aactgtaatg ggagacgata
    60301 attttatcac atggttattg tctggtataa ttgtaaagat aaacgtgtta gtagtattat
    60361 cggcaaaact attatattct tgaatatcta tagatggctc actattgtaa aaccatattt
    60421 tctccatctg aagactatgt tcatttaccc tccagtgacc atgttgcagg aaatagatga
    60481 tatgcatgcc aaaatcgtta tctatttgcg aatcattatc cagcttatca atttcaacta
    60541 tctttgaacg atgtgtttta tcaaccttgc tatggtgttg atgattatta actttatctg
    60601 aatgcttatc tttatggttt gaagggatgt acttaaatat attgtcaatt gctttcataa
    60661 ataacccgcg atatttttga cctgttagta aaaaataaat gatacatgtc tgaaagacat
    60721 atttacgacg atataaaaca ataatactta tatgttcgct gattttaagc accgagacta
    60781 tccggtcttt tactaaataa tggaaaacaa tccatccctg gaagacaatt gtaaaaaaaa
    60841 acaacagaat aagaattaat cttaggataa atatttattt atcatggcct tttgaccgct
    60901 aaaaaatggc actcggcaca gtaaaccccg ctatagttat gctatgcatt tgtctttaat
    60961 gatatctcgt cgttatatta acaggctgaa attatcatta atgcacattt caatattagc
    61021 agggatacct gaggagttta aagtgaacat atatatcggg tagcttttca aattaatccc
    61081 tttgattata ggcttaattt gcatcgcgtt aggtggcttt gtgctggaaa gttcagggca
    61141 acgtcacggg tcatgtgctg atttctctgg cggccatatg cctggcatta ttcactaccg
    61201 catttattat catttcgcaa ctcacgcgcg gcgttaatac gttttacaat acattgttcc
    61261 ccattattgg ctatgcgggg tcaattatca ccatgatatg gggttgggca ctgttagcag
    61321 gtaatgatgt gatggcagac gagtttgttg ccggccatgt tatttttggc gttggtatga
    61381 ttgccgcatg tgtatcgacg gtggcagcgt cgtccggtca ctttctgctc attcccaaaa
    61441 atgcagcggg gagcaagagc gacggaacac cggtacaggc ttattcttca ttaatcggta
    61501 attgcctcat tgccgttccc gttttactca ccctgctcgg tttcatttgg tctattacgc
    61561 tgttacatag tgccgacata actccgcatt atgtcgcctt gggttaaccg caatctgtgc
    61621 ctgtctaatt ggcctagtgg ccacaatcgt ccatcaaaca cgtaatacgt tctcatccaa
    61681 agagcactgg ctgtggtgtt attgggttat ttttctcggc tcagtcacgg tgctgcaggg
    61741 gatatacgtc ttagtcagtt ccgatgcaag cgcccgactg gctcccggca ttattctcat
    61801 ttgcctcgga atgatctgtt acagcatatt ctcaaaagtc tggctactgg cactggtctg
    61861 gagacgtacc tgttcgttag ccaacagaat accgatgata ccggtattta cctgtctgtt
    61921 ttgcttgttt ttgtcatcat tcctcgcgga aatcgcacaa actgagctgg actactttat
    61981 tccatctcgg gttctggttg gattaggcgc agtatgtttt acattgttct ctattgtttc
    62041 aatattagag gccggttctg cgaaaaaaaa ttaaaattta cataatccca gcgttattca
    62101 tatcttaacg ctggtttttt acgaacttag tgaaataatg tagaatataa aattataatt
    62161 ttatattgga gcacatgtgt taatgatcaa ccccgggata ctaaaaatat agacattatt
    62221 aaacttgctt agcctcgcat tgcctttacc tgttaaagat gcatcgagaa aattgagtct
    62281 gaagttctca tcctttatct gattttccag tcccgaaact ttggccttag cattacctga
    62341 gatccgtccg ttactatcta ttttgtaggt aaaaaaatac gaatgggaaa atttcctcgg
    62401 tgtatcttca tcagttaagc caacaatcgt aaaactaccg ctaccatctt tttttaaaat
    62461 gactgaaagg ttagcagagt agtataaaga atgatctgtt ttagcatttc ttgttaacgt
    62521 cgacgtacat tcaagagacg ttgtgctacc atcacgagcc ttccagagag caaaaaaaca
    62581 cacctgtagc aatgtgaaaa caagtattaa tataaaatac attttcttat tagtcatata
    62641 atagcacctc tgaacaaatt gcataagttt cgtcattaga ggattttata catcttgata
    62701 aataccgctg cccttcccca agcaaataat gcatactatt tacatcatat ataaaatttg
    62761 cacctggcac acagggtaaa tgctttttcg aaatttcagc ggcctctatt tgagatttag
    62821 cgaggccaat ctcgttaaga ttctctgaaa gatataaatc acaggtgtca atcttacctg
    62881 atttaaccaa tggataatcc gttatactgt aggtatcttt aaaagccacg gcaaacattt
    62941 caataataac aattactacc acaagcgaaa tcaaaatgcg aaaaagagga atactccttt
    63001 tgcgctctac tttttcaaca acatcttctt gtaaatgaga atcatcataa gattgtttac
    63061 ttattgttat gttttcactg atgtaaaagc ctactttggg tatcgtaaga atcacatttt
    63121 cttcaacacc aatattttta aaacttttgc gtaacaggga tatatattgg ttcaatgtgt
    63181 tgtttgacag atcaaatgaa aatctctcca aaacgctatc aatgattgag ttcctgtcaa
    63241 ccgctccacc attactttcc agtaacactg ccaaaataca ccccgccgta gctggaaaat
    63301 ttatctcatc ctgggtattt acattcgaga gcgagcattt gttcggatca aaaataatcg
    63361 cgtcattgat aatgaaaaaa catctccgct ccatgcaggc aacaacacaa aataagattt
    63421 tgtcatttta gcctattcct ggtggaactg acacaagaaa ctaaaataca aatataaccc
    63481 tgaaaaagtc atataaaact tatatccaca gaataaaaaa tcaaacatgt cagttttatc
    63541 aatataaagt atcgcaaggc gaaaagtgat gcaagaatac tcctaagtaa atgaaagcgc
    63601 accacatgaa attatcaaca catgtaattt catcattagg tgctatcgaa aataactcag
    63661 gagctactta agatgaataa tgtcttactc atgagacccg atgactcaga atttatgata
    63721 cctgatgact ggtcagggct aaacgcaaaa ggcattacat acgaacaaga taatagagtg
    63781 acagagtctt ttctgccttt taaatctttt tattgttcac cggtgtgcac gacagttgaa
    63841 ctcatctcac aaaataattg ttttgctatg ggtatccgct accttctgga aaagaaaaga
    63901 gacttaaggc taaatcactt taaagccttc tctcataaat gcgagacaaa acctgacgtg
    63961 ttaattatga ccgggtgcac cgtcgctgat acttatagta tattctcacg cattgttgaa
    64021 gaaaatataa tgaaaacgat gacacctatc gtcatcatta ctgggcatca ctcagaagat
    64081 ctttgttctc tggcatcgaa actgggagga gttaaaataa tatcaccgac agaaaagatt
    64141 gcaaaaatag aaaacgctat caatgatgct tttaacagca agcctaaaaa aataagattt
    64201 ttaacagata tactctctcc tcaacaaatt gagaccatga aaatggttgc gcagggtaga
    64261 tccgttaatg agatttcagc cgcaatgaat attagcatca agaccgtgta tgcacataaa
    64321 gttcgtgcta tcaataagct gggaattaag aaaaaagttt atgaagcgct tttttataaa
    64381 gcgctaattc agttcaactg atgtaaaaat caccctcgct taataaataa tatattctat
    64441 gtcttttttt ataagttaaa aaaggcggga tatcaaatcc cgccatgaaa ttaactaatc
    64501 atctgcatta ttctgcgtag atataagcct gggtacggat ggtgtaagag tctggctcaa
    64561 caacctgttc tttcatatgg gaaacgacgc gataggcacc acctgttcca gtatttttca
    64621 gcatcatata acgcttaccg ccaatctctt cggaggcagc tgaaggagga tcctgctcaa
    64681 gcgttgtcag ggaaactttc agtttatggt ccgggttatt gtcaccagaa attactgcat
    64741 ccgctgaagt actgctctcc gcataaatat catccgggaa agttacagca tagtcgtggc
    64801 tctgcccatc agatgcgcca acaatacctg ttgccaaagg cgtccatgca gttaacgtac
    64861 cgctatatac ccctttcact ggttgaatgg tattattaac aacgataggc ttggtcaaag
    64921 taagattagc tgacgcttca gtaccctggc ttactaaagt tcccgcctga gaagcaaaag
    64981 ctgtccccgc cagcatgatt gctaacaatg ttttcttcat tatattttct ccatgatttt
    65041 catttacact gcaaaatgca gtttctttct ttgcatttac gtgctatcgc gaaaatgctt
    65101 ccaccaaata atggaaacat cttcacaaaa ataaattatg agtttcttaa tttagaaact
    65161 ataaacatgt cgccgctatc tggtataacc ctgatgcact tttcttcgca ggaagttggt
    65221 attttgcgat acaggattga tctttagaat aaccccattt cacattaaac tcgccttccg
    65281 cactcaagcc agaaacataa gcaataccat cttcacctac aatgccggcc atattttcag
    65341 tgtctttgct ataactaatc acagcgccga aggggatggg tttaccctct ttctgtttta
    65401 aggttactaa tcctttatcc ccgctcttca ctgccaatgt cgcaggcaca acggctccag
    65461 ctgtcggtac aaccaattga ctagtttgtg gtaagtcgta attcttacct atcgtttttt
    65521 catccagaat aacatcgtct cttcggtatg gctgcactgg cagtattgca tacccacgaa
    65581 aatcagtact cagtcccggc cagccattaa cgttaacccc cgttgctccg ggtgcctcta
    65641 taagggctaa tgtcccatcc acattttgac caaatgtaag tccatccctt gtaataagta
    65701 tcccaccgct taaatttgca cctagctgtt tcatgttttc gctggagctg taatggctac
    65761 taaactgacc atatgatcca tttttgccaa cttgcacaat gttcatcgtg ctcttcggtt
    65821 gtgactgatg agcgttagtt atatttgtcg ctacatccca ccatacacca ccactattct
    65881 gacctgataa tgaaacctga tagttattac cctgctctcc cgaacgactc atctgtgaag
    65941 acgcataaat ttgattttcg tcacccagcg ataatggcac tgaaataccc acgcttaatt
    66001 tccgatctac tttccattta tgcgatgtca gcaagtcgtt ttttgcccag ttaatggaga
    66061 atgttatgcc tctccatacc gtactataac tcaggctggt agaccgcgtg tttcctgtgc
    66121 cccgggaaga attatacctc gtggtattga agttcaaatt cccgatatta ccaaggggct
    66181 gggataagga tatcgtagag ccatcgtgac tattcttatc tctttttctg ttccgttcat
    66241 cctgtgcact tactgcttca gtcagtgtgg cataacctga tgcataacgg ttcgtggtta
    66301 tagaca
//
LOCUS       DAJTPG010000009        65690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTPG010000009 DAJTPG010000000
VERSION     DAJTPG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(130..609)
                     /gene="folK"
                     /locus_tag="OO230_000639"
     CDS             complement(130..609)
                     /gene="folK"
                     /locus_tag="OO230_000639"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5690516.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO230_000640"
     CDS             complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO230_000640"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5690517.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO230_000641"
     CDS             complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO230_000641"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5690518.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO230_000642"
     CDS             complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO230_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5690519.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO230_000643"
     CDS             complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO230_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5690520.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO230_000644"
     CDS             complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO230_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5690521.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4982..7411
                     /gene="hrpB"
                     /locus_tag="OO230_000645"
     CDS             4982..7411
                     /gene="hrpB"
                     /locus_tag="OO230_000645"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5690522.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7607..10141
                     /gene="mrcB"
                     /locus_tag="OO230_000646"
     CDS             7607..10141
                     /gene="mrcB"
                     /locus_tag="OO230_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5690523.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10361..12604
                     /gene="fhuA"
                     /locus_tag="OO230_000647"
     CDS             10361..12604
                     /gene="fhuA"
                     /locus_tag="OO230_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5690524.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12655..13452
                     /gene="fhuC"
                     /locus_tag="OO230_000648"
     CDS             12655..13452
                     /gene="fhuC"
                     /locus_tag="OO230_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5690525.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13452..14342
                     /gene="fhuD"
                     /locus_tag="OO230_000649"
     CDS             13452..14342
                     /gene="fhuD"
                     /locus_tag="OO230_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5690526.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14339..16321
                     /gene="fhuB"
                     /locus_tag="OO230_000650"
     CDS             14339..16321
                     /gene="fhuB"
                     /locus_tag="OO230_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5690527.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16356..17636)
                     /gene="hemL"
                     /locus_tag="OO230_000651"
     CDS             complement(16356..17636)
                     /gene="hemL"
                     /locus_tag="OO230_000651"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5690528.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17861..19282
                     /gene="clcA"
                     /locus_tag="OO230_000652"
     CDS             17861..19282
                     /gene="clcA"
                     /locus_tag="OO230_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5690529.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19306..19371
                     /locus_tag="OO230_000653"
     CDS             19306..19371
                     /locus_tag="OO230_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690530.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19364..19708
                     /gene="erpA"
                     /locus_tag="OO230_000654"
     CDS             19364..19708
                     /gene="erpA"
                     /locus_tag="OO230_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5690531.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19755..20378)
                     /locus_tag="OO230_000655"
     CDS             complement(19755..20378)
                     /locus_tag="OO230_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5690532.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20416..21216)
                     /gene="btuF"
                     /locus_tag="OO230_000656"
     CDS             complement(20416..21216)
                     /gene="btuF"
                     /locus_tag="OO230_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5690533.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21209..21907)
                     /gene="mtnN"
                     /locus_tag="OO230_000657"
     CDS             complement(21209..21907)
                     /gene="mtnN"
                     /locus_tag="OO230_000657"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5690534.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21991..23508
                     /gene="dgt"
                     /locus_tag="OO230_000658"
     CDS             21991..23508
                     /gene="dgt"
                     /locus_tag="OO230_000658"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5690535.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23638..25062
                     /gene="degP"
                     /locus_tag="OO230_000659"
     CDS             23638..25062
                     /gene="degP"
                     /locus_tag="OO230_000659"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5690536.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25217..26373
                     /gene="cdaR"
                     /locus_tag="OO230_000660"
                     /pseudo
     CDS             25217..26373
                     /gene="cdaR"
                     /locus_tag="OO230_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26462..26848)
                     /locus_tag="OO230_000661"
     CDS             complement(26462..26848)
                     /locus_tag="OO230_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5690537.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27162..27986)
                     /gene="dapD"
                     /locus_tag="OO230_000662"
     CDS             complement(27162..27986)
                     /gene="dapD"
                     /locus_tag="OO230_000662"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5690538.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28017..30689)
                     /gene="glnD"
                     /locus_tag="OO230_000663"
     CDS             complement(28017..30689)
                     /gene="glnD"
                     /locus_tag="OO230_000663"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5690539.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30751..31545)
                     /gene="map"
                     /locus_tag="OO230_000664"
     CDS             complement(30751..31545)
                     /gene="map"
                     /locus_tag="OO230_000664"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5690540.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31913..32638
                     /gene="rpsB"
                     /locus_tag="OO230_000665"
     CDS             31913..32638
                     /gene="rpsB"
                     /locus_tag="OO230_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5690541.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32896..33747
                     /gene="tsf"
                     /locus_tag="OO230_000666"
     CDS             32896..33747
                     /gene="tsf"
                     /locus_tag="OO230_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5690542.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33894..34619
                     /gene="pyrH"
                     /locus_tag="OO230_000667"
     CDS             33894..34619
                     /gene="pyrH"
                     /locus_tag="OO230_000667"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5690543.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34911..35468
                     /gene="frr"
                     /locus_tag="OO230_000668"
     CDS             34911..35468
                     /gene="frr"
                     /locus_tag="OO230_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5690544.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35560..36756
                     /gene="ispC"
                     /locus_tag="OO230_000669"
     CDS             35560..36756
                     /gene="ispC"
                     /locus_tag="OO230_000669"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5690545.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36945..37703
                     /gene="ispU"
                     /locus_tag="OO230_000670"
                     /gene_synonym="uppS"
     CDS             36945..37703
                     /gene="ispU"
                     /locus_tag="OO230_000670"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5690546.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37716..38573
                     /gene="cdsA"
                     /locus_tag="OO230_000671"
     CDS             37716..38573
                     /gene="cdsA"
                     /locus_tag="OO230_000671"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5690547.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38585..39937
                     /gene="rseP"
                     /locus_tag="OO230_000672"
     CDS             38585..39937
                     /gene="rseP"
                     /locus_tag="OO230_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5690548.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39967..42399
                     /gene="bamA"
                     /locus_tag="OO230_000673"
     CDS             39967..42399
                     /gene="bamA"
                     /locus_tag="OO230_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5690549.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42521..43006
                     /gene="skp"
                     /locus_tag="OO230_000674"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42521..43006
                     /gene="skp"
                     /locus_tag="OO230_000674"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5690550.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43010..44035
                     /gene="lpxD"
                     /locus_tag="OO230_000675"
     CDS             43010..44035
                     /gene="lpxD"
                     /locus_tag="OO230_000675"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5690551.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44140..44595
                     /gene="fabZ"
                     /locus_tag="OO230_000676"
     CDS             44140..44595
                     /gene="fabZ"
                     /locus_tag="OO230_000676"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5690552.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44599..45387
                     /gene="lpxA"
                     /locus_tag="OO230_000677"
     CDS             44599..45387
                     /gene="lpxA"
                     /locus_tag="OO230_000677"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5690553.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45387..46535
                     /gene="lpxB"
                     /locus_tag="OO230_000678"
     CDS             45387..46535
                     /gene="lpxB"
                     /locus_tag="OO230_000678"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5690554.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46532..47128
                     /gene="rnhB"
                     /locus_tag="OO230_000679"
     CDS             46532..47128
                     /gene="rnhB"
                     /locus_tag="OO230_000679"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5690555.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47165..50647
                     /gene="dnaE"
                     /locus_tag="OO230_000680"
     CDS             47165..50647
                     /gene="dnaE"
                     /locus_tag="OO230_000680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5690556.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50660..51619
                     /gene="accA"
                     /locus_tag="OO230_000681"
     CDS             50660..51619
                     /gene="accA"
                     /locus_tag="OO230_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5690557.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51718..53859
                     /gene="ldcC"
                     /locus_tag="OO230_000682"
     CDS             51718..53859
                     /gene="ldcC"
                     /locus_tag="OO230_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5690558.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53916..54305
                     /locus_tag="OO230_000683"
     CDS             53916..54305
                     /locus_tag="OO230_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5690559.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54370..55668
                     /gene="tilS"
                     /locus_tag="OO230_000684"
     CDS             54370..55668
                     /gene="tilS"
                     /locus_tag="OO230_000684"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5690560.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55717..55977)
                     /gene="rof"
                     /locus_tag="OO230_000685"
     CDS             complement(55717..55977)
                     /gene="rof"
                     /locus_tag="OO230_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5690561.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55964..56164)
                     /locus_tag="OO230_000686"
     CDS             complement(55964..56164)
                     /locus_tag="OO230_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5690562.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56330..56875
                     /locus_tag="OO230_000687"
     CDS             56330..56875
                     /locus_tag="OO230_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5690563.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56872..57294
                     /gene="arfB"
                     /locus_tag="OO230_000688"
     CDS             56872..57294
                     /gene="arfB"
                     /locus_tag="OO230_000688"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5690564.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57308..58018
                     /gene="nlpE"
                     /locus_tag="OO230_000689"
                     /gene_synonym="cutF"
     CDS             57308..58018
                     /gene="nlpE"
                     /locus_tag="OO230_000689"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5690565.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58173..58997)
                     /locus_tag="OO230_000690"
     CDS             complement(58173..58997)
                     /locus_tag="OO230_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5690566.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59050..60768)
                     /gene="proS"
                     /locus_tag="OO230_000691"
     CDS             complement(59050..60768)
                     /gene="proS"
                     /locus_tag="OO230_000691"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5690567.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60879..61586)
                     /gene="tsaA"
                     /locus_tag="OO230_000692"
     CDS             complement(60879..61586)
                     /gene="tsaA"
                     /locus_tag="OO230_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5690568.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61583..61987)
                     /gene="rcsF"
                     /locus_tag="OO230_000693"
     CDS             complement(61583..61987)
                     /gene="rcsF"
                     /locus_tag="OO230_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5690569.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62105..62920)
                     /gene="metQ"
                     /locus_tag="OO230_000694"
     CDS             complement(62105..62920)
                     /gene="metQ"
                     /locus_tag="OO230_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5690570.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62960..63613)
                     /gene="metI"
                     /locus_tag="OO230_000695"
     CDS             complement(62960..63613)
                     /gene="metI"
                     /locus_tag="OO230_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5690571.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63606..64637)
                     /gene="metN"
                     /locus_tag="OO230_000696"
     CDS             complement(63606..64637)
                     /gene="metN"
                     /locus_tag="OO230_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5690572.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64825..65400
                     /gene="gmhB"
                     /locus_tag="OO230_000697"
     CDS             64825..65400
                     /gene="gmhB"
                     /locus_tag="OO230_000697"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5690573.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15534 a        16277 c        18098 g        15781 t
ORIGIN      
        1 gatgggattg atatttatct gcgggaattt atattatgtt tctgcaaaaa tgtttaaact
       61 aaaattttta ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat
      121 ataaataaat taccatttgc ttaatttgtc aaatgctctt gtatgtaaga cttcacgcaa
      181 cgtctcccca tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa
      241 tccacgattc ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt
      301 accaaacagc atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac
      361 acgaccctgt tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgggcga
      421 agtttcaagc gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg
      481 ggtgcggtaa aacgaagaaa cggcaagaat gcggctttca gggatatcgc ctaatgcttt
      541 cagggcagca ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc
      601 cactgtcatg tggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacgacga
      661 cgcggtgacg gttcttcatc cagctcgtta agcatccctt tttgatctgg tggtgcggaa
      721 acctggaact caccccacca tttcaccaga cgctgcagtt cagcattacg ttcaacttca
      781 gctcgcaagg ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat
      841 gcgcgtttac cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac
      901 gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc
      961 gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct gggcgatctt ctgtgccgtc
     1021 tccagcagtg ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg
     1081 ttatggatac gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg
     1141 tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat
     1201 tcacacaaca gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct
     1261 tcaaacaggc gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct
     1321 gcggtttccg ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata
     1381 cgtaccggat cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc
     1441 ttcagatcct tcataccacc aacgtaatca cggacggtaa aatccgctac gctgtaatac
     1501 aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg
     1561 tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct
     1621 tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg ggccaaacat tacatgagcc
     1681 agacggaaac ggcgacccac caggcggcag ttacggaaca gtttgcgcac ctgctcaggc
     1741 gtggcgttag tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg
     1801 ccgccgccaa ccagccaggc ttcgtatccc gctttgttga gcctgtacat taccttcagg
     1861 gcattttcac tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc
     1921 tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa
     1981 aaattagcga ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa
     2041 aaaaatagcg gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg
     2101 ccgactcggg cacggcggtc agcatccagt ttttgacggc tgactgaagg atttgctcga
     2161 cgctgaaatc ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta
     2221 gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag
     2281 cgccttgtgg attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa
     2341 gctggtacag cgggatttgc cttactgttg gttcaatcag atcagcccca cgcactattt
     2401 ctgtaacgcc ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat
     2461 cacggcgatg aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgcctc
     2521 gcagcaggtc agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt
     2581 ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat accgccaatg ctttgaatac
     2641 gcgcacgcgt acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt
     2701 cacgataggc gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt
     2761 aatgttccag ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga
     2821 tatcttctat gcgtaccagc cagcgacctt gccgggcgcg agcctgcaaa tagctgccga
     2881 gcgcggcgat cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa
     2941 tatactgtgt gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt
     3001 ggtaaacgtg atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca
     3061 gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa
     3121 tttcaacacc gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct
     3181 tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact
     3241 cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat
     3301 gtgtaacggt gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac
     3361 ggaagtgcgc cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt
     3421 ccaccccagc gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca
     3481 tgttgcttct ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct
     3541 ataaatagca gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt
     3601 gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact
     3661 acaatgtaac cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt
     3721 aagccagaat ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat
     3781 cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaaaataa
     3841 ccgcacgctg gccttcagcc gctacgctca tcaactcccg aaggtgtttc tgacctcgtt
     3901 cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt
     3961 caatatagca gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttcgg
     4021 agccgtattt tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat
     4081 taaggatagc ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc
     4141 cgctctggct ttgagttaat tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag
     4201 tcgaatacca gacggtatcg ccaggcgttg cacaaccggt catcgcaccc gtattcgggc
     4261 agtgtagcgt taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac
     4321 gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc cattgcgaca atccttgtta
     4381 ttgcgttagc gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga
     4441 ggcgtaaagg gtgaactccg tcaccgcata agaccagtta aaacccggcg gcgggaatgt
     4501 caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg
     4561 aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg
     4621 tggaggctga cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc
     4681 aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct aaaagagaaa gcgccttctc
     4741 tttctctgcg ctcacttcgc ctaaaaatgc cagagtcagg tgcaaattat cggcggcgac
     4801 cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccaa tggataatct gttcgcggat
     4861 ttctgcaggt aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg
     4921 gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt
     4981 tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc
     5041 gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca
     5101 actgctggcg catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc
     5161 ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac gaaaagccag gcgataccgt
     5221 tggctaccgg atgcgtgcgc aaaactgcgt cgggctgaat acccgcctgg aagtggttac
     5281 cgaaggcgtg ctgacgcgga tgatccagcg tgacccggaa ctgagcggtg ttggattggt
     5341 gatccttgat gagtttcatg agcgcagttt gcaggcggat ttggcgttgg cgctgttact
     5401 cgatgtgcaa caaggtctgc gtgatgacct taaactgctg attatgtcgg ctacgctgga
     5461 caatgaccgc ttgcagcaaa tgctgccgga agcgcccgtc gtcatctcag aagggcgctc
     5521 gtttccggtt gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgaagccgt
     5581 tgcggtagcc accgccgaaa tggtgcgtca ggaaagtgga tcattactgt tatttttacc
     5641 tggcgtcgga gaaattcagc gcgtgcagga acaactggct tcgcgcatcg gcagtgatgt
     5701 ggtgctctgc ccgctgtatg gcgtgttgtc actgaacgat cagcgaaaag cgatcctccc
     5761 ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac
     5821 catcgaaggt attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gctttgatcc
     5881 gcgcacgggg cttacgcggt tgattactca acgcgttagt caggcatcca tgacgcagcg
     5941 tgccgggcgc gccgggcgtc tggagccggg tatcagcctg catttaatcg ccaaagaaca
     6001 agcagaacgc gccgcggcgc aaagtgagcc ggagatctta caaagcgatc tttccggttt
     6061 gctgatggaa ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca
     6121 accgccaacg gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga
     6181 gggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt
     6241 agcggcaatg ctggttaacg caaagagcga tgacgaagct gctaccgcgg caaaaattgc
     6301 cgccattctc gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg
     6361 caatcaacca gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgcgg
     6421 cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat
     6481 tgctcatcgc cgtgggcaag atggacgcta tcaactggca aacagcatgg gagcgatgct
     6541 cgatgccgac gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg
     6601 cagcgcctcg ccggatgcgc ggattttact ggcgctaccg gtcgatattg atgaattagt
     6661 acaacgctgt ccgcagctgg tgcagcaatc tgacaccgtg gaatgggatg acgcgcaagg
     6721 tacgctgaaa gcctggcgtc ggctacaaat cggtcagttg acggtgaaag tgcagccgct
     6781 ggcgaaaccg tcagaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg
     6841 tttaagcgtg ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc
     6901 cgcaaagtgg ttgccggaat atgactggcc agcggttgat gatgaaagtt tgttggcgac
     6961 gctggaaacg tggctgctgc cacatatgac cggcgtacat tcactacgcg gcctgaaatc
     7021 actcgacatt tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga
     7081 tagtgaattg cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca
     7141 tgaagataac ccgcccgcgc tggcggtgag aatgcaggag atgtttggtg aggccactaa
     7201 tccgacgatc gcccaggggc gcgtgccgct ggtgctgaag ttgctttccc ctgcccaaag
     7261 gccgctgcaa atcacgcgtg atttgggggc cttctggaaa ggggcgtatc gtgaggtgca
     7321 aaaggagatg aaagggcgtt atcccaaaca cgtctggccg gacgacccgg caaacacggc
     7381 accgacgcga cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag
     7441 agggccgggg tgagggcatc agcgcgcacg ttcaccctca ccctaaccct ctccctcaag
     7501 ggagagggga ccgatcgagc gcaaattttg agagatatct tcttctgtct tgtaacagaa
     7561 gaacagaaaa tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga
     7621 ccgcgagcca attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg
     7681 tcgtcgttac gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc
     7741 gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg
     7801 gctatggcta ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
     7861 ttatctcgat caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcccgcggc
     7921 agtttatggc cgaatggtca accttgaacc agatatgacc atcagcaaga acgaaatggt
     7981 gaagctgctg gaggcaacac agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
     8041 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa
     8101 agaagggcag gtgcgtgcgc gtctgacctt tgatggtgat catctggcga ccatcgtcaa
     8161 tatggagaac aaccgtcagt tcggtttctt ccgtcttgat ccgcgtctga tcaccatgat
     8221 ctcctcgcca aacggtgagc agcgtttgtt tgtgccgcgc agtggtttcc ctgatttgct
     8281 ggtggatact ttgctggcga cagaagaccg tcatttttac gagcatgatg gaatcagtct
     8341 ctactccatt gggcgtgcgg tgctggcaaa cctgaccgca ggtcgcacgg tgcagggtgc
     8401 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gttcttactg
     8461 gcgtaaagcg aacgaagctt acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     8521 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     8581 cggcttcccg ctggcaagct tgtattactt tggtcgcccg gtagaagagc taagcctcga
     8641 ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     8701 cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat
     8761 tattgatcaa gaactctatg acatgttgag tgcccgtccg ctgggggttc agccgcgtgg
     8821 tggggtgatc tctcctcagc cagcctttat gcagctggtg cgtcaggagt tgcaggcaaa
     8881 actgggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     8941 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     9001 taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg
     9061 tgcgatggtc ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     9121 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa
     9181 aatctatcgc ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     9241 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
     9301 ggtggatgcg ttgacacgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg
     9361 gctgcctgcg gttacggaga cctggatcaa actgggcgta ccgaaagatc agttgcatcc
     9421 ggttccggca atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcatt
     9481 ccagaccatc gccagcggtg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc
     9541 ggaagatggt aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     9601 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca
     9661 gcttggggcg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     9721 cgtagatacc tggtttgcag gcattgacgg cagcacggtg accatcacct gggtaggccg
     9781 tgataacaac cagccgacca aactgtatgg agccagcggg gcaatgtcga tttatcagcg
     9841 ttatctggct aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga
     9901 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
     9961 ctggaccagc gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg
    10021 caatccgttt gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca
    10081 agagcagaaa gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta
    10141 acatctaagc gtgaaatacc ggatggcgag ttgccatccg gtaaaataac atcccatcta
    10201 agatattaac cctttctttt catctggttg tttattaacc cttcaggaac gctcagattg
    10261 cgtaccgctt gcgaacccgc cagcgtttcg aatattatct tatctttata ataatcattc
    10321 tcgtttacgt tattattcac tttacatcag agatatacca atggcgcgtt ccaaaactgc
    10381 tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat
    10441 gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc
    10501 acctgcgccg caagaaagcg catgggggcc tgctgcaact attgcggcgc ggcagtcagc
    10561 taccggcact aaaaccgata cgccgattca aaaagtgcca cagtctattt ctgttgtgac
    10621 cgccgaagag atggcgctgc atcagccgaa gtcggtaaaa gaagcgctta gctacacgcc
    10681 gggtgtctct gttggtacgc gtggcgcatc caacacctat gaccacctga tcattcgcgg
    10741 ttttgcggca gaaggccaaa gccaaaataa ctatctgaat ggcctgaagt tgcagggcaa
    10801 cttctataac gatgcggtca ttgacccgta tatgctggaa cgcgctgaaa ttatgcgtgg
    10861 cccggtttcc gtgctttacg gtaaaagcag tcctggcggt ctgttgaata tggtcagcaa
    10921 gcgtccgacc accgaaccgc tgaaagaagt tcagtttaaa gccggtactg acagcctgtt
    10981 ccagactggt tttgacttta gcgatgcgtt ggatgatgac ggtgtttact cttatcgcct
    11041 gaccggtctt gcgcgttctg ccaatgccca gcagaaaggg tcagaagagc agcgttatgc
    11101 tattgcaccg gcgttcacct ggcgtccgga tgataaaacc aatttcacct tcctttctta
    11161 cttccagaac gagccggaaa ccggttatta cggctggttg ccgaaagagg gaaccgttga
    11221 gccgctgccg aacggtaagc gtctgccgac agactttaat gaaggggcga agaacaacac
    11281 ctattctcgt aatgagaaga tggtcggcta cagcttcgat cacgaattta acgacacctt
    11341 tactgtgcgt cagaacctgc gctttgctga aaacaaaacc tcgcaaaaca gcgtttatgg
    11401 ttacggcgtc tgctccgatc cggcgaatgc ttacagcaaa cagtgtgcgg cattagcgcc
    11461 agcggataaa ggccattatc tggcacgtaa atacgtcgtt gatgatgaga agctgcaaaa
    11521 cttctccgtt gatacccagt tgcagagcaa gtttgccact ggcgatatcg accacaccct
    11581 gctgaccggt gtcgacttta tgcgtatgcg taatgacatc aacgcctggt ttggttacga
    11641 cgactctgtg ccactgttca atctgtacaa tccggtgaat cccgatttcg acttcaatgc
    11701 caaagatccg gcaaactccg gcccttaccg cattctgaat aaacagaaac aaacgggcgt
    11761 ttatgttcag gatcaggcgc agtgggataa agtgctggtc accctaggcg gtcgttatga
    11821 ctgggcagat caagaatctc ttaaccgcgt tgctgggacg accaataaac gtgatgacaa
    11881 acagtttacc tggcgtggtg gtgttaacta cctgtttgat aatggtgtaa caccttactt
    11941 cagctatagc gaatcgtttg aaccttcttc gcaagttggg aaggatggta atattttcgc
    12001 accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat gtaccggaag atcgtccgat
    12061 tgtagttact ggtgccgtgt ataatctcac taaaaccaac aacctgatgg cggaccctga
    12121 gggttccttc ttctcggttg aaggtggcga gatccgcgca cgtggcgtag aaatcgaagc
    12181 gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct tatacttaca ccgatgcgga
    12241 atacaccacc gatactacct ataaaggcaa tacgcctgca caggtgccaa aacacatggc
    12301 ttcgctgtgg gctgactata ccttctttga cggtccgctt tcaggtctga cgctgggcac
    12361 cggtggtcgt tatactggct ccagctatgg tgatccggct aactccttta aagtgggaag
    12421 ttatacggtc gtggatgcgt tagtgcgtta tgatctggcg cgagtcggca tggcgggctc
    12481 caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa tacgtcgcca gctgctttaa
    12541 cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa cctttcgttt
    12601 ctaatttctc ttttggggca cggatttccg tgcccatttc acaagttggc tgttatgcag
    12661 gaatacacga atcattccga taccactttt gcactgcgta atatctcctt tcgtgtgccc
    12721 gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt gaccggtctg
    12781 attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggtcg tcatcagcct
    12841 ccgtcagaag gggagattct tcttgatgcc cagccgctgg aaagctggag cagcaaagcg
    12901 tttgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg gatgaccgtg
    12961 cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cgctggggcg ctttggtgcg
    13021 gcagatcgcg aaaaggtcga ggaagctatc tcgctggttg gcttaaaacc gctggcgcat
    13081 cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc catgctggtg
    13141 gcgcaggata gccgttgtct gttgctcgac gaaccgactt cagcgctgga tatcgcccac
    13201 caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg aacgtggcct gacggtcatt
    13261 gccgtgttgc acgatatcaa tatggcggca cgctactgtg attatctggt cgccctgcgc
    13321 agtggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga aaccctcgaa
    13381 atgatttatg gcatcccgat gggtattttg ccgcatccgg cgggtgctgc acctgtgagt
    13441 tttgtttatt gatgagcggc ttacctctta tttcgcgccg tcgactgtta acggcgatgg
    13501 cgctttctcc gttgttatgg cagatgaata ccgcccacgc ggcggctatt gaccccaatc
    13561 gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc atcgtgcctt
    13621 acggcgtggc ggataccatc aactatcgcc tgtgggtcag cgaaccacca ttgccggact
    13681 cagtgattga cgtcggtttg cgtacagaac ctaaccttga actgctgacc gaaatgaaac
    13741 cgtcgtttat ggtctggtcg gcaggatatg gcccttcatc agaaatgctg gcacgtattg
    13801 cgccgggtcg cggatttaac ttcagtgacg gcaaacatcc gttggcgatg gcgcgtaaat
    13861 cgctgacgga aatggcagat ttacttaacc tgcaaagcgc agcggaaacg catttagcgc
    13921 aatacgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt gcacgcccgt
    13981 tactgctgac gacgcttatc gatccgcgcc atatgctggt cttcggtcca aacagcttgt
    14041 tccaggaaat tctcgatgag tacggcatcc caaatgcctg gcaaggggaa accaacttct
    14101 ggggcagtac cgccgtcagt atcgatcgtc tggcagcgta taaagacgtt gatgtgctct
    14161 gttttgatca cgacaacagc aaagacatgg atgcgctaat ggcaaccccg ctgtggcagg
    14221 ccatgccgtt tgtccgtaca gggcgctttc agcgcgtacc cgcagtctgg ttttatggtg
    14281 caacgctttc ggcaatgcac tttgtgcgtg ttctggataa cgccatcgga ggtaaagcgt
    14341 gagtaaacga attgcgcttt tcccggcgtt attgctggcg ctgttagtga ttgtcgctac
    14401 ggcgctcacc tggatgaact tctcacaggc gctgccgcgc agtcagtggg cgcaggccgc
    14461 ctggtcgccg gatattgacg tcatcgagca gatgattttt cactacagct tgttgccgcg
    14521 tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc tgtttcagca
    14581 agtactgcgt aacccgctgg cggaaccgac gacgttgggc gttgcaacag gcgcgcaact
    14641 ggggattacc gtcaccacgc tctgggcgat ccctagcgcg atggcgagcc agtttgctgc
    14701 gctggcaggg gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg ggaaacggct
    14761 gtcgccggta acgctgattt tcgcggggtt ggtagtgagc ctttattgcg gcgcaatcaa
    14821 tcagttactg gttatcttcc atcatgacca gttgcaaagc atgtttctgt ggagcactgg
    14881 aacgctgacg caaaccgact ggggcggtgt tgagcgttta tggccgcagt tgctgggcgg
    14941 tgtgatgctg acgttgctgc tacttcgtcc gttaaccctg atggggcttg atgatggcgt
    15001 ggcgcgcaat ctcgggctgg ccttgtcgct tgcgcgtctg gcggcgctgt cgctggcgat
    15061 tgtcatcagt gcgctgctgg tgaacgctgt ggggattatc ggctttatcg ggttgttcgc
    15121 gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg ccacgactga tgctggcgtc
    15181 gttgattggt gcgctgatcc tctggctttc cgatcaaatc atcctctggc tgactcgcgt
    15241 gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc ggtgcgccgc tgctactgtg
    15301 gctgctgccg cgtttacgca gcattagcgc gccggatatg aaggttaacg atcgtgtcgc
    15361 gactgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgt tgttgatggc
    15421 tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg cgagcggggc
    15481 gttgctcgag gatttaatgc cctggcgctg gccgcgaatt atggcggcgc tgtttgcggg
    15541 cgtcatgctg gcggtagcgg gctgtattat tcagcgactg accggcaacc cgatggcaag
    15601 cccggaagtg ctggggatta gctccggcgc ggcgtttggt gtggtgttaa tgctgtttct
    15661 ggtaccgggt aatgcctttg gctggttgtt acccgcaggc agtctcgggg cggcggtgac
    15721 gctgttgatc attatgatcg ccgccggacg cggtggattt tccccacacc gcatgttact
    15781 ggcggggatg gcgttaagta ccgcgttcac catgcttttg atgatgttgc aggcaagtgg
    15841 tgacccgcga atggcgcaag tgctgacctg gatttccggt tcgacctaca acgcgaccga
    15901 tgctcaggtc tggcgcaccg gaattgtgat ggtgattttg ctggcgatta ctccgctgtg
    15961 ccgccgctgg ctgaccattt taccgctggg tggtgatacc gcgcgggcgg tagggatggc
    16021 gctgacgccg acgcgaattg cgctgctgct gttagcggct tgcctgacgg cgaccgcgac
    16081 gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg cgcggatgat
    16141 gggctttcga cggacgatgc cacacatcgt aatttcggcg ctggtgggtg gtttactgct
    16201 ggtgttcgct gactggtgtg ggcggatggt gctgtttcca ttccagatcc cggcggggct
    16261 gctgtcgacc tttatcggtg cgccatattt tatctatttg ctgagaaagc agagccgtta
    16321 atagaatgcc tcgcccggtg ggcgaggcaa aattatcaca acttcgcgaa cacccgacgt
    16381 gcagcatcga tggtgttatt gatatcttcc atgctgtgcg ccacagacat aaagcctgct
    16441 tcaaacgctg acggtgccag gtaaacacct tcatccagca tcatatggaa gaaacgctta
    16501 aaacgctcga cgtcacaggc catcacatcc tgatagcacg tcacggactc ggcgtcggta
    16561 aagaagatac cgaacatacc accgacgtgg ttaacgacca gcggaattcc cgcttcttct
    16621 gccgcttcca gcagaccttc tgccagacgt gttgtcagct catccagcgt ttcgtgaatg
    16681 cccggctgcg cgacttcatt cagacaggcg aaacccgctg ccatcgcaat cgggttaccg
    16741 gaaagcgtac ccgcctgata gaccggacct gtcggggcca gcgcatccat tacatcacga
    16801 cgaccaccga atgcgcctac cggcattcca ccgccgatga tttttccgag gcaggttaaa
    16861 tctggcacta cggcgaaata atcctgtgcg ccagctagcg ctacgcggaa accggtcatc
    16921 acttcatcga tgatcagcaa cgcgccaaat tcgtcgcaca gcgcacgcag acctggcagg
    16981 aactctggca gcggcggaac acagttcata ttgcctgcca ccggctcgac gataatacag
    17041 gcaatctctt gcgggtattg ctcaaacgcg gcgcgtacag aggccagatc gttataagta
    17101 caggttaagg tatgtttggc gaaatctgcc ggaacgcccg gcgagtttgg ctggcctaac
    17161 gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag cgtgaccgtg gtaacaacct
    17221 tcaaatttaa tgattttgtc gcgaccggta aaaccacggg ccaggcggat ggcgctcatc
    17281 gtcgcctcgg tgccggagtt caccatgcgc accatatcca tggtcgggac cagttcggtc
    17341 accagttgcg ccattttcac ttccatttcg gtcggtgcac caaagcttaa accacgctcg
    17401 gcggcttcaa tcacggcatt gcggatcgcc ggatggttat ggcccagcac catcggcccc
    17461 caggaaccga cataatcgat ataggctttg ccatcaacat cgtacagata agcgccgtcc
    17521 gctttttcga taaacagtgg agtgccgccc acgccagtaa aggcgcgaac aggggagttc
    17581 acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat tttcagactt actcatggag
    17641 ggttcctgat tcgtagaaaa agtgaatggc tgctattcca tgttattcat aacaagttaa
    17701 atactcgtca aacatcaggc tgcttgtact ggtgcaatcc tgatttctta gagtataaaa
    17761 gttttgtgca tttgaaacat tacgctttgc aaaggatttt catggaacgt gcgagtaaaa
    17821 tgccgtcatc ttatttgtat gaccaataag tgatcattgg atgaaaactg atactccctc
    17881 tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga
    17941 gcgcgataaa accccgttag ccattttgtt tatggcggca gtcgtcggca cgcttgtcgg
    18001 gctggctgcg gttgcttttg acaaaggtgt cgcctggttg cagaaccaac gtatgggggc
    18061 gctggtacat actgctgata attatccgct actgttaacc gtcgcttttc tctgttcggc
    18121 ggtgctggcg atgttcggct actttttggt gcgcaaatac gcgcccgaag cagggggatc
    18181 ggggatcccg gaaattgaag gggcgctgga agatcaacgt cctgttcgct ggtggcgtgt
    18241 attgccggtg aaattctttg gcgggctggg gacactcggc ggaggcatgg ggctagggcg
    18301 cgaaggacca accgtgcaga tcggcggcaa tatcagtcgc atggtgcttg atattttccg
    18361 cctgaaaggt gatgaagctc gccatacgct gctggcaacc ggtgctgctg cggggctggc
    18421 tgcggccttt aacgcgccgc tggcgggtat tttgtttatt atcgaagaga tgcgtccgca
    18481 gtttcgctat acgttaattt cgattaaagc ggtatttatt ggcgtcatta tgtcgaccat
    18541 tatgtaccgg atttttaatc atgaagttgc gttgattgac gtcggtaaac tttctgatgc
    18601 gccgcttaat acgctgtggc tttatctgat cctcggtatt atttttggca ttttcggccc
    18661 tatttttaat aaatgggtgc tggggatgca ggatttgctg caccgtgtgc acggcggcaa
    18721 tattaccaaa tgggtactga tggggggggc gattggcggt ctgtgtggat tgctggggtt
    18781 tgtggcacca gcaacgtcgg gcggcggttt taacctgatt cctatcgcta ccgcggggaa
    18841 tttcagcatg ggaatgctgg tgtttatctt cgtcgctcgg gtcattacca ccttactctg
    18901 cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg ctggcgctgg gtactgtgct
    18961 gggaaccgct ttcggaatgg ttgccgttga gctgtttccg caatatcacc tggaggcggg
    19021 gacgtttgct attgccggaa tgggggcatt actggcggca tctattcgcg cgccgttaac
    19081 ggggatcatt ctggttctgg agatgaccga taactaccag ctcattttgc caatgattat
    19141 taccggtctt ggcgcaacac tattagcgca atttaccggc gggaaacctc tatactcggc
    19201 gattcttgcg cgcacgctgg caaaacagga agctgagcaa ctggcgcgaa gcaaggccgc
    19261 atcagccagc gagaatactt gaacgaaata ccagggtatt agataatggc gattattatt
    19321 gggttagaat ttgcccgatt gccgatgtcg tttggagcaa aatatgagtg atgacgtagc
    19381 actgccgctg gagtttaccg acgcagcagc caacaaagtt aaaagcctga tcgctgacga
    19441 agataacccg aatctgaaat tacgcgtgta tatcaccggt ggcggttgca gcggcttcca
    19501 gtatggtttc acctttgatg atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg
    19561 cgttggcctg gtggttgatc cgatgagcct gcaatatctg gtcggcggtt ccgttgacta
    19621 caccgaagga ctggaaggtt ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg
    19681 cggttgcggt tcttccttta gcatctaatc tgttgttcat gtgccggata aactatccgg
    19741 cacatcttct ctcttcaacg cccattctca tccagcgcaa acgtcggtag cttaagatgc
    19801 caacgaatag ccgccagccg aatcaatagc gtcacgacca tgcccatcat actggctgtt
    19861 tccagtggta cggaaaatgt gtaataagcc gtagcgtgga caataccgcc gataatacag
    19921 gcagttgcgt agatttctgt acgtaaaatc atggggattt cgcggaccag tacatcacga
    19981 atgatcccgc cgccaacgcc agtaatgacg cccatacaaa ccgcgattaa cggaccggct
    20041 tccgcattaa aggctttatt cacgctaatg ccgacaaaca ccgccagacc aacggcgtcc
    20101 agcaccggca acatccattt tggtaagcgt cttggctggc gcaccagcac gatggtcagc
    20161 atgctggtga ccatggcaac gaccagatcg gtgggatctt tcacccaaaa taccgggccg
    20221 tgatccagcg ccatgtcgcg aattgttccg tcgcctactg cggtaaccac gcccagtacc
    20281 agaacaccaa aagggtccat acgcaatttt ccggctaaca aaacgccgta gatggcaaat
    20341 accgctgtgc cgactatatc cagccaatag acgagcattg ttcaatcccc actgagcacc
    20401 tgaaaaggtc aggcactaat ctacctgtga aagcgcatta cagagctgtt gtgctgcgag
    20461 gataatacgt gggcttgcac gttcaaacca gtcactcgtg agaggaataa cgggaatttt
    20521 gagctgttca ccccagtatt gtttgatttt aggaatttgg tccggtccgc ctgtaatgac
    20581 aatagcctgt ggcgagcgtg ctaacacctg ttcgcggcta acttgcggcc agggaacccg
    20641 gctgtcttta aagatgtttt ctccgccaca aacttcgagt acctggttct gaatcgactc
    20701 ttttccactg gtaaatggcg gattaatgcc gaattgcaga aaaacacgtt ttttaggttt
    20761 atcagcatat tgcgctttca attgcacgta ctgatccagc agggattgcg cggcttgttc
    20821 ggccttgtct ggttgcggac tccagggggc cagttgacgt aacgcattgg caatttgttc
    20881 aatgcttgtc gcatcgaccc acatcacttt tattcccagc gaagccagct ggtcaacctg
    20941 ccgctcggca ttacctccac gccaggcaat caccagatcg ggtttcagcg cgacaatgcg
    21001 ttccagattc atcccctgcc aggtggaaac ctgctcaatc ttttgcgctt gtggaggata
    21061 gtcggaatag ctgctgaccc caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt
    21121 ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc cacagtggcg cgagaaaaga
    21181 cagggcgacc agcgccctga acagtgattt agccatgtgc cagtttctgc accagtgact
    21241 caaccatcag gctggactgt ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag
    21301 actgttgatc ggccacgtcg gagatggcgc gtacgacaac aaacgggacg ttgaaattgt
    21361 ggcagacatg ggcgattgcc gtcgcttcca tctctacagc aatggcctgt gggaagttgt
    21421 ggcggatttt cgccagacca acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc
    21481 cacgtacagc gttaagattc agttcggcaa tgcaggcctc agcggcagcg atcagtttat
    21541 cgtcagcttt aaagcctgcc ggacagcctg gtaactgacc gtattcataa ccaaatgccg
    21601 tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac gatatcgccc actttcagcg
    21661 ttggtgccag gccaccggca gaaccggtgt taataatcac atctggcttg cagtgttcca
    21721 acagcagagt ggcacccagc gccgcagcga ctttaccgat gcccgatttc agaagcgcaa
    21781 cctcggttcc attcagttgg ccggtataga tttcgcaacc gccgagactg atagtttgac
    21841 ggttttcgat tttgtcacgc agcagcgtaa cttcttcttc cattgcacca atgatgccga
    21901 ttttcataga tttactcgcg ataagcccga tttgaaggca tagtttacca tgcgcttacg
    21961 gggaagcgta tttctcacgc gggagaggac atggcacaga ttgatttccg aaaaaaaata
    22021 aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg
    22081 atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg caattcgtcg tctgcaacaa
    22141 aagacccagg tttttccact ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg
    22201 atggaagtcc agcaggtggg acgctacatc gccaaagaaa ttttaagccg tctgaaagag
    22261 cttaaattgc tggaagcata cggcctggat gaactgaccg gcccctttga aagcattgtt
    22321 gagatgtcat gcctgatgca cgatatcggc aatccgccgt ttggtcattt tggcgaagca
    22381 gcgataaatg actggtttcg ccaacgtttg cacccggaag atgccgaaag ccagcctctg
    22441 actgacgatc gctgcagcgt ggcggcacta cgtttacggg acggggaaga accgcttaac
    22501 gagctgcggc gcaagattcg tcaggactta tgtcattttg aggggaatgc acaaggcatt
    22561 cgtctggtgc atacattgat gcggatgaat ctcacttggg cacaggttgg cggtatttta
    22621 aaatataccc gtccggcgtg gtggcgtggc gaaacgcctg agacacatca ctatttaatg
    22681 aaaaagccgg gttattatct ttctgaagaa gcctatattg cccgcttgcg taaagaactt
    22741 aatttggcgc tttacagtcg ttttccatta acgtggatta tggaagctgc cgacgacatc
    22801 tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatttac cgttgagcag
    22861 ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct cttttcgctg
    22921 gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag tacggaagat
    22981 cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta cgcggcacaa
    23041 cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc attattggaa
    23101 gatgccagcg aatgcagcga tcttcttaag ctatataaaa atgtcgctgt aaaacatgtg
    23161 tttagccatc cggatgtcga gcagcttgaa ttgcagggct atcgggtcat tagcggatta
    23221 ttagagattt atcgtccttt attaagcctg tcgttatcag actttactga actggtagaa
    23281 aaagaacggg tgaaacgttt ccctattgag tcgcgcttat tccacaaact ctcgacgcgc
    23341 catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc tgagtttccg
    23401 ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac
    23461 ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc aggcttttgt
    23521 aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact tcgggctata
    23581 aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact gaaatacatg
    23641 aaaaaaaaca cattagcact gagtgcactg gctctgagtt taggtttggc gttatctccg
    23701 ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat gccaagcctt
    23761 gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga aggtagcaca
    23821 accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga tgattctccg
    23881 ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggggg ccagggcggt
    23941 aatggcggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc
    24001 gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt gattaaagtc
    24061 cagctgagcg atggtcgtaa gttcgacgcg aagatggttg gcaaagatcc gcgctctgat
    24121 atcgcgctga tccaaatcca gaacccgaaa aaactgaccg caattaagat ggcggattct
    24181 gatgcactgc gcgtgggtga ttacaccgta gcgattggta acccgtttgg tctgggcgag
    24241 acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc tgaaaactac
    24301 gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg tgcgctggtt
    24361 aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga cggcggcaac
    24421 atcggtatcg gttttgctat cccgagtaac atggtgaaaa acctgacctc gcagatggtg
    24481 gaatacggcc aggtgaaacg cggtgagctg ggtattatgg ggactgagct gaattccgaa
    24541 ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct
    24601 aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc actgaacggt
    24661 aagccgatca gcagctttgc cgcactgcgt gctcaggtgg gtactatgcc ggtgggcagc
    24721 aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct ggaactgcag
    24781 cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga aggcgctgag
    24841 atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca acgtgaagac gggcactccg
    24901 gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg gtgcgaacca gcaggcagtg
    24961 aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct ggcactgaac
    25021 attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctccctc aaccccttcc
    25081 tgaatacggg aaggggttct ccttacaatc tgtgaacttc gccacaactc catacatctt
    25141 catcatcctt tgggcatttg cacaatgccg tacgttacgt acttccttat gctaagccgt
    25201 gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc aggatatcgt
    25261 ggcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc gcgggcgaat
    25321 tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggtgcat tgctggtact
    25381 ttcacaggga cgagtcgtcg atatcgatga cgcggtagca cgtcatctgc acggtgtgcg
    25441 gcaggggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa ttggcctgac
    25501 aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg ctgaaatgat
    25561 gctggaacag tcgcggttga tgcacttgtt ggcgcaggat agccgtttgc gggaagaact
    25621 ggtgatgaac ctgattcagg cagaggagaa tactcccgca cttctgaatg ggcgcaacgg
    25681 ctggggatcg atctcaatca accgcgagtg gtggctattg ttgaggtcga cagcggtcag
    25741 cttggcgtgg acagcgcaat ggcggagtta caacaactgc aaaacgcgct gactacgccc
    25801 gagcgtaata atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg
    25861 ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta agcgagttga acaactgatt
    25921 acccgcatga aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa ctattttacc
    25981 ggtcctggca gtattgcccg atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa
    26041 cagcggatgc cagaaagtcg ctgctatttt tatcaggatc tgatgttacc tgtgttactc
    26101 gacagtttgc gtggcgactg gcaggccaac gaactggcgc gaccgctggc gcggctgaaa
    26161 gcgatggaca ataacggctt gctgcgacga acgctggcgg cgtggtttcg ccacaatgtg
    26221 caaccgctgg caacgtcaaa ggcgttgttt attcatcgta ataccctgga gtatcggctt
    26281 aatcgtatat cggaactgac cgggcttgat ttgggcaatt ttgatgacag gttgctgctg
    26341 tatgtggcgt tacaactgga tgaagagcgg tagattatgc gttaaggttg aggcggcgct
    26401 ggctcattcc cctcagcccg aaggggagag gggaatgccc gagttaaccg tcaattcggg
    26461 attatttacg cgttagtttt tccagatcgg cttcaatctc gctgatctta ttggttacga
    26521 ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagatcaact tcgctgcggt
    26581 cacgctgaca gatttgatca agctcatcaa tgatataccg tagattcggg ctgatttcct
    26641 ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc tgacgcggat
    26701 atttaaactt aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa tagattttca
    26761 gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca ggattggtaa
    26821 tacccagact tttcagattg tcgtacatag cgttacctca aaatgagtca gtaaattgtg
    26881 cttattttag catttggctt ggcccgcccg gctcgactgt gatcgcagaa agcctttttc
    26941 ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg tggcgatatt
    27001 ccatgggatg gattagcaca tttatcttcg ttttgcgaat tacatgacag tccgaaacaa
    27061 gatgaaaaag tatttaaaaa agggggagga agaatgagta ggacgaatta ttctgagcca
    27121 gaaagcatac gggcagcatc atgctgcccg tatatgtact cttaatcgat ggtacgcagc
    27181 agttcgttaa tgccgacttt gccgcgagtt ttcgcgtcaa ctttcttaac gataaccgca
    27241 cagtagaggc tgtatttgcc atcttttgac ggcagattac ctgaaacaac cacagacccc
    27301 gccggaacgc gaccgtagtg gatttcgccg gtttcacggt cgtaaatacg ggtgctctga
    27361 ccaatgtata cgcccataga gatgacggaa ccttcttcga caatcacccc ttcaaccact
    27421 tcagagcgtg cgccaatgaa gcagttatct tcaataatgg tcgggttagc ctgcagcggc
    27481 tccagcacgc cgccgatgcc cacgccaccg gaaaggtgga cgtttttacc aatctgcgca
    27541 caagaaccga cggtcgccca ggtatcaacc atggtgcctt catcaacata tgcgccgatg
    27601 ttaacgtaag acggcatcag cacggtgtta cgggcaataa acgcaccctg acgtaccgcc
    27661 gctggtggca caacgcggaa gccttctttc tggaaacgtg cttcgtcgta gtcggcgaat
    27721 ttcatcggca ctttgtcgaa gtagcggctt tctgcccctt cgatcacctg attatcatta
    27781 atacggaaag agagcagcac cgcttttttc aaccactgat gcgtcaccca ctgaccgtca
    27841 attttttccg ctacacgcag tgcgccggaa tccagcaggg cgatcacctg atttaccgct
    27901 tcgcgggtaa cggtgtctgc attggctggc gtgatctcgg cacggcgttc aaaagcggtt
    27961 tcaataatgt tctgtaactg ctgcattgtt aaactctttt catatcagta aacacatcac
    28021 cctttatcgt ttggattgag ggcctctgtc aaccgctgat gcacttcctg ctgcaactcg
    28081 ttattaagcg cacgccggtc agcggtggca attatgaata aatcttctac tcgctcgcca
    28141 atggttgtaa ttcgggcacc atgaagcgaa attcccagat cggcaaaaat tttcccgact
    28201 cgcgccagca gtccaggttg gtcgagggcg atcagttcga ggaacgattt gcggtcggta
    28261 tgggtcggca aaaacgttac ttcggtttca acagtaaaat ggcgtaattt ggcgggttgg
    28321 cgacggggct gcggtggctg ccagctactt tgcgtcagca cctgctccag accaaaccga
    28381 ataacctcat ggcgatctgc ggacagcggg ctgccatcgg gttccagcac gataaaggta
    28441 tccatcgcca taccgtcgcg agtggtgaaa atttgtgcgt cgtgaacact taaattgcgg
    28501 cggtctaatt cggcacagac ggcggcaaac agataggggc ggtccgggct ccagataaaa
    28561 atctcggtgc ctccacgcgt agcctgcggg ctaagcaata ccagcggttt gcttaaatca
    28621 tgctgtaata aatggcgggc atgccaggcc agttgatttg ggctatggcg gacaaaatag
    28681 ttagcacgac agcgtgacca aatttggtgc agcgcctctt cgtcgatgtt atccatgcgc
    28741 agtagtgcca gtgcctggag ttggtgatgg cgaacccgtt cgcgcatatc cggcgtgttt
    28801 tgcattccgc gtcgtagctg cttttcggtg gcaaaataga gttcgcgcaa cagactttgc
    28861 ttccagctat tccacagtgt ttcgttggtg gcgcaaatgt cagccactgt caggcatacc
    28921 agatagcgca gacgattttc cgtttgaact tcttcggcaa actgcttgat gacttcaggg
    28981 tcctgaatat cgcggcgttg tgctgtcacc gacatcagca agtgctggcg aaccagccag
    29041 gcaaccagct gtgtttcgcg tgagttcagc ccgtggagtt ctgcaaaatg cactacatcc
    29101 tgagcaccga gaatggagtg gtcgccgccg cgtcctttgg cgatatcgtg aaacagcgcg
    29161 gcgatgaaaa tcagctcagt tgacggcagg cgcggccaga cgtccacaca caacggatgg
    29221 cgctggcgcg tttcttcact ggcaaaactc tccagtttca gcatcacgcg gatagtatgt
    29281 tcatccaccg tgtaggcgtg gaacagatca aactgcatct gcccgacgat atgcgaccat
    29341 tgcggcatat acgcgccgag cacgctatgg cgatgcattg gcaatagtcc gcgccgcacc
    29401 gctccggggt gacgcagaat gctcaaaaac agttttcgtg cttgcggaat attacacagc
    29461 ggttgttgca gatggcgacg ggcatggcgt aactggcgca gcgtggtgga gtaaatgccg
    29521 gtgatcgcac tgttgcgcac catggtgtag aacatacgca agatggcttc cggctggcgc
    29581 ataaatagtg tttcatcacg caggtcgatt agcgtaccgc gtagctgaaa ctcatcgtcg
    29641 agtggacgtg gtttttcgtc ggcgggaagg gcgaggatgg cttcatcgaa cagttgcagc
    29701 agcatctggt tgagttcact gacgcggcgt gtaacgcgga agtaatcctt catcatccgc
    29761 tcgaccggtt cgttgccttc accactgtaa ttcagacgct gagcgacgct aagttggcga
    29821 tcgaataaca gacgattatc gtaacggctg acgaccagat gcagggcaaa gcgaatacgc
    29881 cacagtatat gcagacattc gtttaattcc gcccgctccg ctgaggttaa gaagccaaac
    29941 ccgaccattt catccagcga tgttgcgcca aaatgacggc gggccaccca ttgcagagtg
    30001 tgaatatcgc gcaagccgcc agggctactt ttgatgtctg gttcaaggtt gtagctggtg
    30061 ccatggtaac gctgatggcg ctggttctgt tcttcaactt tcgctgcgta gaacttgtcg
    30121 gaaggccaga atccttcgct aaaaatatgt ttttgcagtt cgaggaagag cgcaacatcg
    30181 ccaattaata agcgggattc gattaaattg gtggcgacgg ttaaatccga taacccttcc
    30241 aacatgcact cttcaagcgt gcgcacacta tgaccgactt ccagctttac atcccagagc
    30301 agcgttaaca gctcgcccac tttttgcgcc tgatcgtccg ggagcttttt acggcttaaa
    30361 atcagtaaat cgatgtcaga aagtggatgc agctcgccac gaccgtagcc accgacggca
    30421 accaatgcca ggtcggcaat ctggctgaat cccgcttcaa tccataatcg ttgcaggagc
    30481 tggtctatga actcggtacg tgcctcaatc aactgttctg cagagatccc attgtcaaag
    30541 gcatcaccca gccaacgctg gaaagtatcg atatgggctt ttatcccaca gacggttaat
    30601 tcatcacggg gccaggcgca tggattttgc ggttgaccgg gcagggtgga gagagcggtg
    30661 tttgcgtact gttctggaag ggtattcatt gtgcgccacc cataagatta aattatcgca
    30721 ttaaaaaagc cggcattcgc cggcttcatc ttattcgtcg tgcgagatta tcgccgggat
    30781 ggtgtcatcc ttgcgtagcg tcaaaatttc gcagccgtta tcagtcacca caatagtatg
    30841 ctcatattgt gcagacaagc tgcgatcttt ggtttttacc gtccagccat ctttcatggt
    30901 gcggatctct tttttacccg cgttgaccat tggctcaatg gtgaacgtca tccccggttt
    30961 cagcacgacg ttggtttcac gggagtcata gtgcagcacc tgcggttctt catggaagcc
    31021 gcgaccaata ccgtgtccgc aatattcacg aacgacggag aagccttctg cttcgacaaa
    31081 tttctgaatc gccgcaccga tttcgcgcag attaatgcct ggttttacca tgcgtagcgc
    31141 caggtacagg ctttcttgcg tgatgcggca caggcgttcg cccatgatgg tcggcttacc
    31201 gacgataaac attttcgagg tatcgccgtg gaaaccatct ttgattacgg tgacatcaat
    31261 gttaacgata tcgccatctt tcagcagctt agcatcgtcc gggataccgt ggcacaccac
    31321 ttcattaata gagatgcaaa cggatttcgg atagccgtga tagccgaggc aggcagaaac
    31381 cgcgtgttgt tcattaacaa tgtaatcatt acagatgcga tccagctcgc cggtgctgac
    31441 gcccggttta acatacggtt cgatcatctc cagcacttcg gcagccagtc ggccagcgac
    31501 gcgcattttt tcgatatctt ctggggtctt gattgagata gccattaatt ctgtccatca
    31561 gcgtcggtga taccgacaat atatatgtaa gtgccgtcaa tggtatcaca cccgggcaaa
    31621 ttgagaatca ttctgaattt cgccaaacgt gccactgaag gttttctata atagaaaatt
    31681 cgacgtctga tgctgtacac agcgccaaca attattggtg tccacgacgt atttgtggta
    31741 taaagcgcgc cggacttccg atccatttcg tatacacaga ctggacggaa gcgacaatct
    31801 cactttgtgt aacaacacac acgtatcggc acatattccg gggtgccctt tggggtcggt
    31861 aatatgggat acgtggaggc ataaccccaa cttttatata gaggttttaa tcatggcaac
    31921 tgtttccatg cgcgacatgc tcaaggctgg tgttcacttc ggtcaccaga cccgttactg
    31981 gaacccgaaa atgaagccgt tcatcttcgg tgcgcgtaac aaagttcaca tcatcaacct
    32041 tgagaaaact gtaccgatgt tcaacgaagc tctggctgaa ctgaacaaga ttgtttctcg
    32101 caaaggtaaa atccttttcg ttggtactaa acgcgctgca agcgaagcgg tgaaagacgc
    32161 tgctctgagc tgcgaccagt tcttcgtgaa ccatcgctgg ctgggcggta tgctgactaa
    32221 ctggaaaacc gttcgtcagt ccatcaaacg tctgaaagac ctggaaactc agtctcagga
    32281 cggtactttc gacaagctga ccaagaaaga agcgctgatg cgcactcgtg agctggagaa
    32341 actggaaaac agcctgggcg gtatcaaaga catgggcggt ctgccggacg ctctgtttgt
    32401 aatcgatgct gaccacgaac acattgctat caaagaagca aacaacttgg gtattccggt
    32461 atttgctatc gttgatacca actctgatcc ggacggtgtt gacttcgtta tcccgggtaa
    32521 cgacgacgca atccgtgctg tgaccctgta cctgggcgct gttgctgcaa ccgtacgtga
    32581 aggccgttct caggatctgg cttcccaggc ggaagaaagc ttcgtagaag ctgagtaata
    32641 aggcttgata actcccccaa aatagttcga gttgcagaaa ggcggcaagc tcgagaattc
    32701 ccgggagctt acatcagtaa gtgaccggga tgagcgagcg aagacaacgc atctgcggcg
    32761 cgaaatatga agggggagag cccttataga ccaggtagta cacgtttggt tagggggcct
    32821 gcatatggcc ccctttttca cttttatatc tgtgcggttt aatgccgggc agatcacatc
    32881 tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct gcgtgagcgt
    32941 actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag
    33001 ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa agcaggcaac
    33061 gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat cattctggaa
    33121 gttaactgcc agactgactt cgttgcaaaa gacgctggtt tccaggcgtt cgcagacaaa
    33181 gttctggacg cagctgttgc tggcaaaatc actgacgttg aagttctgaa agcacagttc
    33241 gaagaagaac gtgttgcgct ggtagcgaaa attggtgaaa acatcaacat tcgccgcgtt
    33301 gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat cggcgttctg
    33361 gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca cgttgctgca
    33421 agcaagccag aattcatcaa accagaagac gtatccgctg aagtggtaga aaaagaatac
    33481 caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc agagaaaatg
    33541 gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca gccgttcgtt
    33601 atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga agtgactggc
    33661 ttcatccgtt tcgaagtggg tgaaggcatc gagaaagttg agactgactt tgcagcagaa
    33721 gttgctgcga tgtccaagca gtcttaatta tcaaaaagga gccgcctgag ggcggcttct
    33781 ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta
    33841 caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga aacatggcta
    33901 ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg
    33961 gcactgaagg cttcggtatt gatgcaagca tactggatcg tatggctcag gaaatcaaag
    34021 aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac ctgttccgtg
    34081 gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg
    34141 cgaccgtaat gaacggcttg gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc
    34201 gtctgatgtc cgctattcca ttgaatggcg tgtgcgacag ctacagctgg gcagaagcta
    34261 tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt aacccgttct
    34321 ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga
    34381 aagcaaccaa agttgacggc gtgtttaccg ctgatccggc gaaagatcca accgcaacca
    34441 tgtacgagca actgacttac agcgaagtgc tggaaaaaga gctgaaagtc atggacctgg
    34501 cggccttcac gctggctcgt gaccataaat taccgattcg tgttttcaat atgaacaaac
    34561 cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc acggaataat
    34621 tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcgggggc gtaagtctgg
    34681 ttataaggcg ttattgttgc aggcagtttg gtcacggcca gcgcgcagca accggagcgt
    34741 acaaaagtac gtgaggatgg cgagcactgc tcggggccaa aatggcaaat aaaatagcct
    34801 aataatccag acgattaccc gtaatatgtt taatcagggc tatacttagc acacttccac
    34861 tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac gtgattagcg
    34921 atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa
    34981 tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat
    35041 attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta
    35101 ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg
    35161 cctccgatct cggcctgaac ccgaactctg cgggtagcga catccgtgtt ccgctgccgc
    35221 cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag
    35281 cgcgtgttgc agtacgtaat gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga
    35341 aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta cagaaactga
    35401 ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca gaactgatgc
    35461 agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga tcggctggcg
    35521 gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact caccattctg
    35581 ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac
    35641 ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg
    35701 gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg
    35761 atgctacagc aacaggggag ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat
    35821 atggcagcgc ttgaggaggt tgatcaggtg atggcagcca ttgttggtgc tgctgggctg
    35881 ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa taaagaatca
    35941 ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc gcaattgtta
    36001 ccggtcgata gcgaacataa cgctattttt cagagtttac cgcaacctat ccagcataat
    36061 ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac cgggtctggt
    36121 ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgcccga tcaagcttgc
    36181 cgtcatccga actggtcgat gggacgtaaa atttctgtcg attcggctac catgatgaac
    36241 aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa
    36301 gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga cggcagtgtt
    36361 ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat ggcatggccg
    36421 aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc gttgacattt
    36481 gccgcaccgg attatgagcg ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa
    36541 ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt
    36601 gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg
    36661 gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgaa cgcgagtgaa
    36721 gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta cagagagtcg
    36781 cgctatttgt tagcgtaggg cttcagtgat atagtctgcg ccatctgatc gtaagtagtt
    36841 ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat aggcttcagt
    36901 attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc
    36961 aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc attatggacg
    37021 gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat aaagccgggg
    37081 caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgag gcgttaacgc
    37141 tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt gcgttaatgg
    37201 aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc
    37261 tgcgtattat tggcgatacc agtcgtttta actcgcgttt gcaagaacgt attcgtaaat
    37321 ctgaagcgct aacagccgga aataccggtc tgacgctgaa tattgcggcg aactacggtg
    37381 gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag caaggaaacc
    37441 tgcaaccaga tcagatagat gaagagatgt taaaccagca tgtctgtatg catgaactgg
    37501 cccctgtaga tttagtaatt aggactgggg gagagcatcg cattagtaac tttttgcttt
    37561 ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat ttcgatgaac
    37621 aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg
    37681 agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc ctgatatctg
    37741 cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg
    37801 ccattgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag cttagcggtt
    37861 ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg ttggcgctga
    37921 tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt gaaatctcac
    37981 tttgggcttc gttgggttgg tggattgtcg cgctattgct ggtgctgttt tacccaggtt
    38041 ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg ctaaccattg
    38101 ttcccttctt ctggggcatg ctggcgttac gggcctggca ctatgacgag aatcattaca
    38161 gtggcgcaat atggctgctc tatgtcatga tcctggtatg gggcgctgac tccggcgcat
    38221 atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct ccgggtaaaa
    38281 cctggcaagg ctttataggt ggactcgcta ctgcagcggt aatctcatgg ggttatggca
    38341 tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt gtcgcagcgt
    38401 tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg
    38461 acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat agcctgacgg
    38521 ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt taacggaagg
    38581 taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg gtgtacttat
    38641 caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg
    38701 tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg gcaccgaata
    38761 tgttatcgcc ctgatcccgt tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc
    38821 ggtcgttccg gaactccgcc accatgcctt caataataaa tctgtcggcc aacgagcggc
    38881 gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct actggctggt
    38941 ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggc gaaatagcag ccaattcgat
    39001 agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg gtatcgaaac
    39061 gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg aaagcaccac
    39121 cattacagta gcgccatttg acagcgacca acggcgggat gtaaagctcg atttacgtca
    39181 ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg
    39241 gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg
    39301 tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc agtgggtgac
    39361 ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa tcgaaaggca
    39421 ggggagtccc ttgtctttga cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat
    39481 tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata aagttgtacg
    39541 ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc agctgatgaa
    39601 gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga acaacctcag
    39661 tgggccgatc tctatcgcca agggggctgg gatgacagcg gaactcgggg tagtttatta
    39721 cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt ttccgttgcc
    39781 cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt
    39841 atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg tgctgttaat
    39901 ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga agaacgcata
    39961 ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag cgccaccgta
    40021 tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca gcgtgtcgcc
    40081 gttggtgcgg ccctcctcag tatgccggtg cgtacaggcg acacggttaa tgatgaagat
    40141 atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt tcgcgtcctt
    40201 cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc cagcattact
    40261 ttctccggta acaaatcggt gaaagatgac atgctgaagc aaaacctcga ggcttctggt
    40321 gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa aggtctggaa
    40381 gacttctact acagcgtcgg taaatatagc gccagcgtta aagctgtcgt gaccccgctg
    40441 ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag
    40501 caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc tcatttccaa
    40561 ctgcgtgacg aagtgccgtg gtggaatgtg gtaggcgatc gtaaatacca gaaacagaaa
    40621 ctggcgggcg atcttgaaac cctgcgcagc tactatctgg accgcggtta tgcccgtttc
    40681 aacatcgact ctacccaggt cagtctgacg ccagataaaa aaggtattta cgtcacggtg
    40741 aacatcaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg caaccttgcc
    40801 gggcactccg ctgaaattga gcaactgact aagatcgagc cgggcgagct gtataacggc
    40861 actaaagtga ccaaaatgga agatgacatt aaaaagcttc tcggtcgcta tggttatgcc
    40921 tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt taaattacgc
    40981 gtgaacgttg atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga aggtaacgat
    41041 acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc atggttgggg
    41101 agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc
    41161 gataccgata cccaacgtgt tccgggtagc ccggaccagg ttgatgtagt ctacaaggtg
    41221 aaagaacgta acactggtag cttcaacttc gggattggtt acggtactga aagtggcgtg
    41281 agcttccagg ctggcgtgca acaggataac tggttaggta caggttatgc tgttggtatc
    41341 aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa cccgtacttc
    41401 accgtagatg gcgtaagcct cggtggtcgt ctcttctata atgacttcca ggcagatgac
    41461 gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt gggcttcccg
    41521 attaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc cctgtccaac
    41581 atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca tccgagcacc
    41641 tctgatcagg ataacagctt caaaacggac gacttcacgt tcaactatgg ttggacctat
    41701 aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct gaccggtaaa
    41761 gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac ggcgacttat
    41821 gtgccgatcg atgacgatca caaatgggtt gttctgggtc gtacccgctg gggttatggt
    41881 gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg tggttccagc
    41941 accgtgcgtg gcttccagtc caataccatt ggtccgaaag cagtttactt cccgcatcag
    42001 gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac
    42061 ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag cctcgagttc
    42121 atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac ttcattcttc
    42181 tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc tggatatccg
    42241 gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca atggatgtcc
    42301 ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga tggagacaag
    42361 gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac aaaggaatgt
    42421 agtggtagtg tagcgatgac tttaggcgat caatataaga tcgccgggcc acgcaaagaa
    42481 ctgcaccctc cggtgcaaat gggatggtaa ggagtttatt gtgaaaaagt ggttattagc
    42541 tgcaggtctt ggtttagcac tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt
    42601 caatatgggc agcctgttcc agcaggtagc gcagaaaacc ggtgtttcta acacgctgga
    42661 aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa
    42721 aatgaaaaaa ctgcagtcca tgaaagcggg cagcgatcgc accaaactgg aaaaagacgt
    42781 gatggctcag cgccagactt ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg
    42841 tcgttccaac gaagaacgcg gcaaactggt tactcgtatc cagactgctg tgaaatccgt
    42901 tgccaacagc caggatatcg atctggttgt tgatgcaaac gccgttgctt acaacagcag
    42961 cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt aaataagtaa tgccttcaat
    43021 tcgactggct gatttagcgc agcagttgga tgcagaacta cacggtgatg gcgatatcgt
    43081 catcaccggc gttgcgtcca tgcaatctgc acaaacaggt cacattacgt tcatggttaa
    43141 cccaaaatac cgtgagcatt taggcttgtg ccaggcgtcc gcggttgtca tgacccagga
    43201 cgatcttcct ttcgcgaaaa gtgccgcgct ggtagtgaag aatccctacc tgacttacgc
    43261 gcgcatggcg caaattttag ataccacgcc gcagcccgcg cagaacattg cacccagtgc
    43321 ggtgatcgac gcgacggcga agctgggtaa caacgtatcg attggcgcta acgcggtgat
    43381 tgagtccggc gttgaactgg gcgataacgt gattatcggt gccggttgct tcgtaggtaa
    43441 aaacagcaaa atcggtgcag gttcgcgtct ctgggcgaac gtaaccattt accatgagat
    43501 ccagatcggt cagaattgcc tgatccagtc cggaacagtg gtaggcgcag acggctttgg
    43561 ttatgccaac gatcgtggta actgggtgaa gatcccacag attggtcgcg taattattgg
    43621 cgatcgcgtg gagatcggtg cctgcacaac catcgatcgc ggcgcgctgg atgacactgt
    43681 tattggcaat ggcgtgatca ttgataacca gtgccagatt gcacataacg tcgtgattgg
    43741 cgacaatacg gcggttgccg gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta
    43801 ctgcatgatc ggcggagcca gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac
    43861 ggttacgggc atgggtatgg tgatgcgtcc catcactgaa ccaggcgtct attcctcagg
    43921 cattccgctg caacccaaca aagtctggcg caaaaccgct gcactggtga tgaacattga
    43981 tgacatgagc aagcgtctga aatcgcttga gcgcaaggtt aatcaacaag actaacgttc
    44041 catcttttgt tcgccaaact ttacggcctg tctcattctt acgattgcgg caggccgtgt
    44101 tattattgtc gtttcttata ttttgacagg aagagtatct tgactactaa cactcatact
    44161 ctgcagattg aagagatttt agaacttctg ccgcaccgtt tcccgttctt actggtggat
    44221 cgcgtgctgg attttgaaga aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat
    44281 gagccattct tccagggcca tttccctgga aaaccgattt tcccgggtgt gctgattctg
    44341 gaagcaatgg cacaggcaac aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg
    44401 ggtgagctgt actacttcgc tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct
    44461 ggcgatcaaa tgatcatgga agtcactttc gaaaaaacgc gccgcggcct gacccgtttt
    44521 aaaggggttg ctctggtcga tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt
    44581 agccgggagg cctgatacgt gattgataaa tccgcctttg tgcatccaac cgccattgtg
    44641 gaagagggcg cgtcaattgg cgcgaacgca cacattggtc ctttttgtat cgttggaccc
    44701 catgtcgaaa ttggtgaggg taccgtactg aaatctcacg ttgtcgtgaa tggtcatact
    44761 aaaattggcc gcgataatga gatttatcag tttgcctcca tcggcgaagt taaccaggat
    44821 ttgaaatatg ctggcgaacc gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa
    44881 agcgtcacca ttcatcgtgg cacagtccag ggcggtggat tgacgaaggt gggcagcgac
    44941 aacttactga tgatcaacgc gcacattgcg cacgattgta cggtaggtaa ccgctgtatt
    45001 ctcgccaaca acgcaacgct ggcgggtcac gtatcggttg acgacttcgc gatcatcggc
    45061 ggcatgaccg cagtccatca gttctgcatc attggtgcgc atgtgatggt tggcggctgc
    45121 tccggtgtgg cgcaggacgt ccctccttat gttattgcgc agggtaacca cgcaacgccg
    45181 ttcggtgtca atatcgaagg gctgaagcgc cgcggattca gccgtgaggc gattaccgct
    45241 atccgcaatg cgtataagct aatttatcgt agcggtaaaa cgctcgatga agtgaaaccg
    45301 gaaattgctg aactggcgga aacatatccg gaagtgaaag cctttaccga tttctttgca
    45361 cgctcaacgc gcggtctgat tcgttaatga ctgaccagcg tccattaacg attgccctgg
    45421 tcgccggaga aacctccggc gatatcctgg gggccggttt aatccgcgct ctgaaagaac
    45481 gtgtgcccaa cgcccgcttt gttggtgttg ccgggccacg aatgcaggct gaaggctgcg
    45541 aagcctggta cgaaatggaa gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc
    45601 tgcgtcgctt actgcatatt cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag
    45661 atgtttttgt tggtattgat gcgcccgact tcaatattac tcttgaaggt aacctcaaaa
    45721 agcagggtat caaaaccatt cattacgtca gtccatccgt ctgggcgtgg cgacagaaac
    45781 gcgttttcaa aataggcaga gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag
    45841 cgttttatga caaatacaat gtaccgtgcc gctttatcgg tcataccatg gctgatgcca
    45901 tgccgttaga tccagataaa aatggtgccc gtgatgtgct ggggatccct tacgatgccc
    45961 actgtctggc attgttgccg ggcagccgtg gtgcagaagt cgaaatgctt agtgccgatt
    46021 tcttgaaaac tgcccagctt ttgcgccaga catatccgga tctcgaaatc gtggtgccgc
    46081 tggtgaatgc caaacgccgc gagcagtttg aacgcatcaa agctgaagtc gcgccagacc
    46141 tgtcggttca tttgctggat ggaatgggcc gtgaggcgat ggtcgccagc gatgcggcac
    46201 tactggcgtc ggggacggca gccctggagt gtatgctggc gaaatgcccg atggtggtgg
    46261 gatatcgcat gaagcctttt accttctggt tggcgaagcg gctggtgaaa actgattatg
    46321 tctcgctgcc aaatctgctg gcgggcagag agttagtcaa agagttattg caggaagagt
    46381 gtgagccgca aaaactggct gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc
    46441 acgcgatgca cgataccttc cgtgaactgc atcagcagat ccgctgcaat gccgatgagc
    46501 aggcggcaca agccgttctg gagttagcac aatgatcgaa tttgtttatc cgcacacgca
    46561 gctggttgcg ggtgtggatg aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc
    46621 tgcggtgatc cttgacccgg cgcgcccgat tgccgggctg aatgattcca aaaagctgag
    46681 cgaaaaacgc cgtctggcgc tctatgaaga gatcaaagag aaagcgttga gctggagtct
    46741 gggccgcgcg gaaccccacg aaatcgacga gctgaacatt cttcatgcga ccatgctggc
    46801 gatgcagcgt gccgtcgctg ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa
    46861 ccgctgcccg aaattaccga tgcctgcgat ggctgtggtg aaaggggata gccgcgtacc
    46921 agaaatcagt gccgcgtcta tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct
    46981 ggatattgtt ttcccgcaat atggttttgc ccaacataaa gggtatccaa ccgcttttca
    47041 tctggaaaaa ctggctgaat atggcgcgac tgaacatcat cgtcgcagct ttgggcctgt
    47101 caaacgcgca ctgggacttg cgtcctgatt cttgtgtcga gattaagtaa accggaatct
    47161 gaagatgtct gaaccacgtt tcgtacacct gcgggtgcac agcgactact cgatgatcga
    47221 tggcttggcc aaaaccgcac cgctggtaaa aaaggcggcg gcgttgggta tgccagcact
    47281 ggcgatcacc gatttcacca acctttgcgg tctggtgaag ttctacggag cgggacatgg
    47341 cgcagggatt aagcctatcg tcggggcaga ttttaatgtc cagtgcgacc tgctgggtga
    47401 tgagttaacc cacctgacgg tactggcggc gaacaatacc ggctatcaga atctggcgtt
    47461 gctgatctca aaagcgtatc agcgcgggta cggtgccgcc gggccgatca tcgatcgcga
    47521 ctggcttatc gaattaaacg aagggttgat ccttctttcc ggcgggcgca tgggcgacgt
    47581 cggacgcagt cttttgcgtg gtaacagcgc gctggtagat gagtgtgtcg cgttttatga
    47641 agaacacttc ccggatcgct attttctcga gctgatccgc accggcaggc cggatgaaga
    47701 aagctatctg cacgcggcgg tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac
    47761 taacgatgtg cgctttatcg acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat
    47821 ccacgacggc tttaccctcg acgatcctaa acgcccgcgt aactattcgc cgcagcaata
    47881 tatgcgtagc gaagaggaga tgtgtgagct gtttgccgac atcccagaag cccttgccaa
    47941 caccgttgag atcgccaaac gctgtaacgt aactgtgcgt cttggtgaat acttcctgcc
    48001 gcagttcccg accggggaca tgagcaccga agattatctg gtcaagcgtg caaaagaagg
    48061 cctggaagag cgtctggcat ttttattccc tgacgaggaa gaacgtctta aacgccgccc
    48121 ggaatatgac gaacgtctgg agactgaact tcaggttatc aaccagatgg gcttcccggg
    48181 ctacttcctc atcgttatgg aatttatcca gtggtcgaaa gataacggcg taccggtagg
    48241 gccaggccgt ggctccggtg cgggttcact ggtggcctac gcgctgaaaa tcaccgacct
    48301 cgatccgctg gaatttgacc tgctgttcga acgtttcctt aacccggaac gtgtctccat
    48361 gcctgacttc gacgttgact tctgtatgga gaaacgcgat caggttatcg aacacgtggc
    48421 ggacatgtac ggtcgtgatg cggtatcgca gattatcacc ttcggtacga tggcggcgaa
    48481 agcggtgatc cgcgacgtag gccgcgtgct ggggcatccg tacggctttg tcgaccgtat
    48541 ctcgaaactg atcccgcccg atccggggat gacgctggcg aaagcgtttg aagccgagcc
    48601 gcagctgccg gaaatctacg aagcggatga agaagttaag gcgctgatcg acatggcgcg
    48661 caaactggaa ggggtcaccc gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc
    48721 gaccaaaatt accgattttg cgccgcttta ttgcgatgaa gagggcaaac atccggtcac
    48781 ccagtttgat aaaagcgacg ttgaatacgc cggactggtg aagttcgact tccttggttt
    48841 gcgtacgctc accatcatca actgggcgct ggagatgatc aacaagcggc gggcgaagaa
    48901 tggcgagcca ccgctggata tcgccgcgat cccgctggat gacaagaaaa gcttcgacat
    48961 gctgcaacgc tcggaaacca cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct
    49021 gatcaagcgt ctgcaacctg actgcttcga agatatgatc gcactggtgg cactgttccg
    49081 ccctggtccg ttgcagtcag ggatggtgga taactttatc gaccgtaaac atggtcgtga
    49141 agagatctcc tatccggacg tgcagtggca gcatgaaagc ctgaaatcgg tactggagcc
    49201 aacctacggc attatcttgt atcaggaaca ggtcatgcag attgcccagg tgctttctgg
    49261 ttataccctc ggtggcgcgg atatgctgcg ccgtgcgatg ggtaagaaaa agccggaaga
    49321 gatggctaag cagcgttctg tatttgctga aggtgcagaa aagaacggaa tcaacgccga
    49381 actggcgatg aaaatcttcg acctggtgga gaaattcgcg ggttacggat ttaacaaatc
    49441 gcactctgcg gcctatgctt tggtgtcata tcaaacgtta tggctgaaag cgcactatcc
    49501 ggcggagttt atggcggcgg taatgaccgc cgatatggac aacaccgaga aggtggtggg
    49561 cctggtggat gagtgctggc ggatggggct gaaaatcctg ccaccagata taaactccgg
    49621 tctttaccat ttccacgtca acgacgatgg cgaaatcgtg tatggtattg gcgccatcaa
    49681 aggggtaggt gaaggtccga ttgaggccat catcgaagcc cgtaataaag gcggctactt
    49741 ccgcgaactg tttgatctct gcgcccgtac cgacaccaaa aagttaaacc ggcgggtgct
    49801 ggaaaaactg atcatgtccg gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat
    49861 gaactcgctg ggtgatgcgt taaaagcggc agatcaacac gcgaaagcgg aagctatcgg
    49921 tcaggccgat atgttcggcg tgctggccga agagccggaa caaattgaac aatcctacgc
    49981 cagctgccaa ccgtggccgg agcaggtggt attagatggg gaacgtgaaa cgttaggcct
    50041 gtacctgacc ggacacccta tcaaccagta tttaaaagag attgagcgtt atgtcggagg
    50101 cgtaaggctg aaagacatgc acccgacaga acgtggtaaa gtcatcacgg ctgcggggct
    50161 cgttgttgcc gcgcgggtta tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct
    50221 ggatgaccgt tccgggcggc tggaagtgat gttgtttact gacgccctgg ataaatacca
    50281 gcaattgctg gaaaaagacc gcatacttat cgtcagcgga caggtcagct ttgatgactt
    50341 cagcggtggg cttaaaatga ccgctcgcga agtgatggat attgacgaag cccgggaaaa
    50401 atatgctcgc gggcttgcta tctcgctgac ggacaggcaa attgatgacc agcttttaaa
    50461 ccgactccgt cagtctctgg aaccccaccg ctctgggaca attccagtac atctctacta
    50521 tcagagggcg gatgcacgcg cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag
    50581 cgatcgttta ttaaacgatc tccgtggcct cattggttcg gagcaggtgg aactggagtt
    50641 tgactaatac aggaatacta tgagtctgaa tttccttgat tttgaacagc cgattgcaga
    50701 gctggaagcg aaaatcgatt ctctgactgc ggttagccgt caggatgaga aactggatat
    50761 taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc gtagaactga cgcgtaaaat
    50821 cttcgccgat ctcggtgcat ggcagattgc gcaactggca cgccatccgc agcgtcctta
    50881 taccctggat tacgttcgcc tggcatttga tgaatttgac gaactggctg gcgaccgtgc
    50941 gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt ctcgatggtc gtccggtgat
    51001 gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa attcgccgta actttggtat
    51061 gccagcgcca gaaggttacc gcaaagcact gcgtctgatg caaatggctg aacgctttaa
    51121 gatgcctatc atcaccttta tcgacacccc gggggcttat cctggcgtgg gcgcagaaga
    51181 gcgtggtcag tctgaagcca ttgcacgcaa cctgcgtgaa atgtctcgcc ttggcgtacc
    51241 ggtagtttgt acggttatcg gtgaaggtgg ttctggcggt gcgctggcga ttggcgtggg
    51301 cgataaagtg aatatgctgc aatacagcac ctattccgtt atctcgccgg aaggttgtgc
    51361 gtccattctg tggaagagcg ccgacaaagc gccgctggcg gctgaagcga tgggtatcat
    51421 cgctccgcgt ctgaaagagc tgaaactgat cgactccatc atcccggaac cgctgggcgg
    51481 cgctcaccgt aacccggaag cgatggcggc atcgttgaaa gcgcaactgc tggcggatct
    51541 ggccgatctc gatgtgttaa gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat
    51601 gagctacggt tacgcgtaat tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt
    51661 tttatcgcca cggtttgagc aggctatgat taaggaagga ttttccagga ggaacacatg
    51721 aacatcattg ccattatggg accgcatggc gtcttttata aagatgagcc catcaaagaa
    51781 ctggagtcgg cgctggtggc gcaaggcttt cagattatct ggccacaaaa cagcgttgat
    51841 ttgctgaaat ttatcgaaca taaccctcga atttgcggcg tgatttttga ctgggatgag
    51901 tacagtctcg atttatgcag cgatatcaat caacttaatg aatatctccc gctttatgcc
    51961 ttcatcaaca cccactcgac gatggatgtc agcgtgcagg atatgcggat ggcgctctgg
    52021 ttttttgaat atgcgctggg gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc
    52081 aacgaatatc ttgataacat tacgccgccg ttcacgaaag ccttgtttac ctacgtcaaa
    52141 gagcggaagt acaccttttg tacgccgggg catatgggcg gcaccgcata tcaaaaaagc
    52201 ccggttggct gtctgtttta tgattttttc ggcgggaata cccttaaggc tgatgtctct
    52261 atttcggtca ccgagcttgg ttcgttgctt gaccacaccg ggccacacct ggaagcggaa
    52321 gagtacatcg cgcggacttt tggcgcggaa cagagttata tcgttaccaa cggaatatcg
    52381 acgtcgaaca aaattgtggg tatgtacgcc gcgccatccg gcagtacgct gttgatcgac
    52441 cgcaactgcc ataaatcact ggcgcatctg ttgatgatga acgatgtagt gccagtctgg
    52501 ctgaaaccga cgcgtaatgc gttggggatt ctggggggga tcccgcgccg tgaatttact
    52561 cgcgacagca tcgaagagaa agtcgctgcc actacgcaag cacaatggcc ggttcatgcg
    52621 gtgatcacca actccaccta tgatggtttg ctctacaaca ccgactggat caaacagacg
    52681 ctggatgtcc cgtcgatcca cttcgattcc gcctgggtgc cgtatactca ttttcatcca
    52741 atctaccagg gtaaaagtgg tatgagcggc gagcgtgttg cgggaaaagt tttcttcgaa
    52801 acgcaatcga cccacaaaat gctggcggcg ttatcgcagg cttcactgat ccacattaaa
    52861 ggcgagtatg acgaagaggc gtttaacgaa gcctttatga tgcataccac cacctcgccc
    52921 agttatccca ttgttgcttc ggttaagacg gcggcggcga tgctgcgtgg taatccgggc
    52981 aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt ttcgcaaaga ggtccagcgg
    53041 ctgcgggaag agtctgacgg ttggtttttc gatatctggc aaccgccgca ggtggatgaa
    53101 gccgaatgca ggcccgttgc gcctggcgaa caatggcacg gctttaacga tgcggatgcc
    53161 gatcatatgt ttctcgatcc ggttaaagtc actattttga caccggggat ggacgaacag
    53221 ggcaatatga gtgaggaggg gatcccggcg gcgctggtag caaaattcct cgacgaacgt
    53281 gggatcgtag tagagaaaac cggcccttat aacctgctgt ttctctttag tattggcatc
    53341 gataaaacca aagcaatggg attattgcgt gggttgacgg aatttaagcg ctcttacgat
    53401 ctcaacctgc ggatcaaaaa tatgctgccc gatctctatg ctgaagatcc cgatttctac
    53461 cgcaatatgc gtattcagga tctggcgcaa gggatccata agctgattcg taaacacgat
    53521 ctttccggtt tgatgttgcg ggcattcgat actttgccgg aaatgatcat gacgccacat
    53581 caggcatggc agagacagat taaaggtgaa gtagaaacca ttgcgctgga acaactggtc
    53641 ggtagagtat cggcaaatat gatcctgcct tatccaccgg gcgtaccgct gttgatgcca
    53701 ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt ttctactgat gctttgttcc
    53761 gtagggcaac attaccccgg ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac
    53821 ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac ttgccagagc ggcttccggg
    53881 cgagtaacgt gctgttaaca aataaaggag acgttatgct gggtttaaaa caggttcacc
    53941 atattgcgat tattgcgacg gattatgcgg tgagcaaagc tttctactgc gatattcttg
    54001 gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga ctcgtggaaa ggggatttgg
    54061 cgcttaatgg gcaatatgtg attgagcttt tctcttttcc gttcccgccg gagcgcccca
    54121 gccgaccgga agcttgcggc ctgcgtcatc tggcttttag cgttgatgac atcgatgcgg
    54181 cagtggcgca ccttgaaagc cataacgtga agtgtgaagc catccgtgtc gatccataca
    54241 cgcaaaaacg cttcaccttc tttaacgatc cggacgggct gccgttggaa ctgtatgagc
    54301 agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc gggcattgct actgtaaaat
    54361 cgcaccatca tgacactcac gctcaataga caacttctca cctcacgcca gattctggtg
    54421 gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc agttggtgca gtggcggacg
    54481 gaaaatccgg gcgtcactct gcgcgctatt catgtgcatc acggtttaag tgccaatgcc
    54541 gatgcctggg ttacgcattg cgaaaacgtc tgccaacagt ggcaggtgcc gctggtggtc
    54601 gaacgcgtac aacttgcgca agaggggctg gggattgagg cccaggcgcg gcaggcacgt
    54661 tatcaggcat ttgcccgtac cctattgccc ggcgaagtgc tggtcaccgc gcaacatctc
    54721 gacgatcaat gtgaaacctt tctgctggcg ctaaaacgcg gtagcggccc tgccgggctt
    54781 tcggctatgg cggaagtttc ggagtttgct ggaacacggc ttatccgccc gttgcttgcc
    54841 cgcacgcggg gggaactgga gcagtgggca ttggcgcatg gtttacgctg gattgaagac
    54901 gaaagtaatc aggacgactc atacgatcgt aactttctgc gcctgcgcgt agtgccgtta
    54961 ttgcagcagc gttggccgca ttttgcggaa gcaacggccc gcagcgccac actttgtgct
    55021 gaacaagaga gtctgttgga tgaactgctg gcggatgatt tagcacactg ccaaacgtcg
    55081 caggggacgc tgcagattgc gccaatgctg gcgatgagtg atgcccgtcg cgcggcgatt
    55141 attcgccgct ggctggcagg gcagaatgca ccgatgcctt cccgcgacgc gctggtgcgc
    55201 atctggcagg aagtggcgct ggcgcgggaa gatgcttccc cctgtttacg tttgggcgcc
    55261 tttgaaatcc gccgctatca gtcgcaactg tggtggatta aatccgtcac cgggcaaagc
    55321 gaaaccattg tgctgtggca gacgtggcta caaccgctgg aattaccggc ggggctggga
    55381 accgtacagc ttactgcggg aggcgatatt cgccctccgc gtgcagacga agcggtcagc
    55441 gtgcgtttca aagcgccagg attgctgcat attgtcgggc gcaacggcgg acgtaagctg
    55501 aagaaaatct ggcaagagtt aggcgtgccg ccgtggctac gtgacaccac gccactgctg
    55561 ttttatggag aaacgctgat tgcggcggca ggggtatttg tgacgcaaga aggtgtggct
    55621 gaaggtgaga atggcgtaag ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga
    55681 taagacgcat caaacgtcgc atccggcgaa agtcaatcag gattcgctta ccaccaccgt
    55741 accgatttca gggtggctaa agctggtaat tttatccaga cgcagctcgc gggtttcgcc
    55801 agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcac tggcttttgc
    55861 ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag
    55921 ctcaagatta tcgtaatcat cacaattaat tggttgatac gtatcattca ttgacataat
    55981 cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg
    56041 cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt
    56101 atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca caatattttt
    56161 ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa gcctacgaag
    56221 ataaactctg tttcgcaagg tgactatacc acactcattt ctgcaatatc agcgccgcaa
    56281 ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta tggcgcttaa
    56341 agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc agtttctcga
    56401 tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgtatga tgctgcgctt
    56461 gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga
    56521 tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc attgatttgt ggattgagct
    56581 ggggctgccg gatgagcgac gaattaagaa agcctgcacc caggccgcag aagtggcgct
    56641 gtttacctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga gcaaatgtgt
    56701 gcagtttgcc aatctttccg tctggtatct ggacgatgaa caactggcga aagtaagcgc
    56761 ctttgccgat cgtaccatga cgctgcaggc aacgattcag gatggtgtta tctggttatc
    56821 ggatgataag aataatctgg aagttaactt aaccgcctgg caacaacctt catgattgtg
    56881 atttcccgac atgttgctat tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag
    56941 ggcgctggcg ggcagcatgt taataagacc tcaacggcta ttcatctgcg ttttgacatt
    57001 cgggcgtcca gcctgccaga gtattacaaa gagcgtctgc tcgccgccag ccatcatttg
    57061 atcagcagtg atggcgtgat tgtcattaag gcacaggaat accgcagtca ggaactgaac
    57121 cgcgaagccg ctctggcccg gctggtggca gtgattaaag atttaacaaa agaacaaaaa
    57181 gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc atcgaaagca
    57241 caaaaatcaa gcgtgaaggc gatgcgcggc aaagtacgca gcggtcggga ataaaaagaa
    57301 ggaatggatg gtgaaaaaag cgatagtgac agcgatggct gtaatcagcc tctttactct
    57361 gatgggatgt aataatcggg ccgaagtcga tacgctttct ccggcgcagg ctgccgaact
    57421 gaaaccgatg ccgcaaagtt ggcgcggcgt gctaccgtgt gccgattgcg aaggaatcga
    57481 aatctctctg ttcctcgaaa aagacggaac atgggtgatg aatgagcgtt atctcggtgc
    57541 tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct
    57601 ggtattaacc gacagcaaag gtgaaaagtc atattatcgg gcgaaaggcg atgcgctgga
    57661 gatgctcgat cgtgaaggca atccaattga atcgcagttc aactatacgc tggaaccggc
    57721 acaatccagt ttacctatga cgccgatgac cctgcgaggc atgtattttt atatggctga
    57781 tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga
    57841 gctggagcgt agctacctgg ctgcgcgcgg tcacagtgaa aaaccggtgt tactgtcagt
    57901 agaaggtcac tttacgcttg aggctaatcc ggatactggt gcgccaacca aagtattagc
    57961 gcccgatacg gcgggtaaat tttacccgaa taaggattgc agtagtttgg ggcagtaaac
    58021 ctgcttaagg cagaaacaaa cgcaaaattg cctgatgcgc tgcgcttatc aggcctacag
    58081 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
    58141 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
    58201 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
    58261 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
    58321 tgcgccagtg taagtggatg cccggatttg gcaaatgcat cagtgacaaa ttccgaacaa
    58381 aaccacgact ttttgtctcc ttcgcacaca ctgcttaact gcgctttcgc caggtcgctg
    58441 acgcactgtt ggcgaaaatc ctcggagaac ggattcagtg agcacatctg gcgagtcacc
    58501 ataaagggaa taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg
    58561 gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa
    58621 agcttatcac tatgcttcat cgctttttta agggaaacaa tctggacgcc agcgcctgtc
    58681 gcttctgcaa cgttattatc acccagaaag attgcaacgt gactcacaga ggaagtgctg
    58741 aagacgcgga ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt
    58801 ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg
    58861 aatttgacag cccatgtttt tgcctcggca tctactgctg tggctgatgg gtctggctgg
    58921 ctgatatcaa cggtacaagc cgaaagtaaa agaaagctgg ggagaagcag gcggcagtac
    58981 gcctttggtt tatccatttt atacaatcca tgtaaaaaag ggccctgaaa ttcaggaccc
    59041 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
    59101 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
    59161 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
    59221 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
    59281 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
    59341 cacctggaac ggcgcgatag cgtcaggcca tacgatgccg cgttcgtcgt agttctgctc
    59401 aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg
    59461 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
    59521 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
    59581 gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg
    59641 atcccagttg ataccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact
    59701 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
    59761 acccggaccg gctttaacca cagcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
    59821 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
    59881 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
    59941 tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt
    60001 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
    60061 tgccagttca atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
    60121 gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
    60181 ctgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
    60241 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
    60301 caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
    60361 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
    60421 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
    60481 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc
    60541 tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac
    60601 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
    60661 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
    60721 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
    60781 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta
    60841 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
    60901 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca
    60961 taggttttgc ccgtttcctc acctttacga taggccgggc gcgggtcctg cgccagtact
    61021 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
    61081 acctctgcgg taaaactcac cgccatctct gcagctggcg cactttgcgc atagctggca
    61141 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta
    61201 ccatcgacca gatccagact gccgagcttc agaatcacgc tgcctttatg gcaaacaacc
    61261 tctttcagct ctaccagcga catgccaatt gggttagggc ggaaagtaga gcgtgttgcg
    61321 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
    61381 ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaatgc ttccaggccg
    61441 cgaacggcgt cggcctggtt gtaggaagct atgagatgca gttcgccgtt ggcgcttttt
    61501 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
    61561 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata
    61621 cagcctgacg atagcagccc ggcgtaccgc tggtgacttc gcagctatgc agtaatacag
    61681 cattggcttt cattttagag gcattgattt gcatctgctt acgtgcggtt ggaatgctcg
    61741 gcggagagtc ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga
    61801 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
    61861 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
    61921 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
    61981 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
    62041 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
    62101 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
    62161 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
    62221 gcgttacgat caggtttacg tatggggagt ctttatcttc gacaaagata ccgtctttcg
    62281 ccggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
    62341 cgtcgtccag agaacgcggc agttgcggtg cttccagttc aacaattttc agattttttg
    62401 ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca
    62461 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
    62521 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
    62581 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
    62641 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
    62701 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
    62761 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
    62821 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
    62881 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
    62941 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcaa
    63001 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
    63061 tgtagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
    63121 cacccatcgc ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac
    63181 ccggcaacgc ttccggtaac agcactttac agacgatctg cattggcgtg gcacccattg
    63241 cgcgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
    63301 caataaacgg tgctgcacca acggttaacg gaacaattgc tgcctgcaaa ccaatcgatg
    63361 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
    63421 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
    63481 gccccggacg cgtgacataa agcagaacgc caaccggcag accaatcaca aacccaaaaa
    63541 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc agccacatca
    63601 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
    63661 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaatttaacg
    63721 ccaccggcgt agtccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
    63781 gtttcagaaa gcagtggggc atcgaccgat tgaccggtaa actccagacg cagcatcggc
    63841 acgcagtcag taaatggctc cgtttgcaga cgttcctggt aatcttccgg gatatccaga
    63901 tgcagggtcg actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca
    63961 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
    64021 cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
    64081 atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
    64141 tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt
    64201 ttctgcccac cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat
    64261 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
    64321 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
    64381 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt caattcctgg
    64441 ccatcgacca gcacgctacc ctcggttggg cgctccagca agtttacaca acgtataagc
    64501 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
    64561 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
    64621 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgta gcatctcgaa
    64681 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
    64741 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
    64801 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
    64861 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
    64921 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
    64981 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
    65041 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
    65101 ccgcatcatc cgcaggggag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca
    65161 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
    65221 atggtgggcg ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa
    65281 gtgctggtgc gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg
    65341 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcgcaatga
    65401 ttaaaagatg aacggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc
    65461 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    65521 ggttgaagag aaaaaatcct gaaattcagg gttgactctg aaataggaaa gtgtaatata
    65581 cgccacctcg cgacagtgag ctgaaagctg cgtcgcaact gctctttaac aatttatcag
    65641 acaatctgtg tgggcactcg aagatacgga ttcttaacgt cgcaagacga
//
LOCUS       DAJTPG010000010        60648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTPG010000010 DAJTPG010000000
VERSION     DAJTPG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            523..1812
                     /gene="sdaC"
                     /locus_tag="OO230_000698"
     CDS             523..1812
                     /gene="sdaC"
                     /locus_tag="OO230_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5690574.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1870..3237
                     /gene="sdaB"
                     /locus_tag="OO230_000699"
     CDS             1870..3237
                     /gene="sdaB"
                     /locus_tag="OO230_000699"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5690575.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3348..4103
                     /gene="xni"
                     /locus_tag="OO230_000700"
     CDS             3348..4103
                     /gene="xni"
                     /locus_tag="OO230_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5690576.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4156..5304)
                     /gene="fucO"
                     /locus_tag="OO230_000701"
     CDS             complement(4156..5304)
                     /gene="fucO"
                     /locus_tag="OO230_000701"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5690577.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5332..5979)
                     /gene="fucA"
                     /locus_tag="OO230_000702"
     CDS             complement(5332..5979)
                     /gene="fucA"
                     /locus_tag="OO230_000702"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5690578.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6526..7842
                     /gene="fucP"
                     /locus_tag="OO230_000703"
     CDS             6526..7842
                     /gene="fucP"
                     /locus_tag="OO230_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5690579.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7875..9650
                     /gene="fucI"
                     /locus_tag="OO230_000704"
     CDS             7875..9650
                     /gene="fucI"
                     /locus_tag="OO230_000704"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5690580.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEASACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9759..11178
                     /gene="fucK"
                     /locus_tag="OO230_000705"
                     /pseudo
     CDS             9759..11178
                     /gene="fucK"
                     /locus_tag="OO230_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11180..11602
                     /gene="fucU"
                     /locus_tag="OO230_000706"
     CDS             11180..11602
                     /gene="fucU"
                     /locus_tag="OO230_000706"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5690581.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11660..12391
                     /gene="fucR"
                     /locus_tag="OO230_000707"
     CDS             11660..12391
                     /gene="fucR"
                     /locus_tag="OO230_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5690582.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12435..13535)
                     /gene="rlmM"
                     /locus_tag="OO230_000708"
     CDS             complement(12435..13535)
                     /gene="rlmM"
                     /locus_tag="OO230_000708"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5690583.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13528..13923)
                     /locus_tag="OO230_000709"
     CDS             complement(13528..13923)
                     /locus_tag="OO230_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5690584.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13942..14859)
                     /gene="gcvA"
                     /locus_tag="OO230_000710"
     CDS             complement(13942..14859)
                     /gene="gcvA"
                     /locus_tag="OO230_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5690585.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15210..15437)
                     /locus_tag="OO230_000711"
     CDS             complement(15210..15437)
                     /locus_tag="OO230_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5690586.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15629..16834
                     /gene="csdA"
                     /locus_tag="OO230_000712"
     CDS             15629..16834
                     /gene="csdA"
                     /locus_tag="OO230_000712"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5690587.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16834..17277
                     /gene="csdE"
                     /locus_tag="OO230_000713"
     CDS             16834..17277
                     /gene="csdE"
                     /locus_tag="OO230_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5690588.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17328..18134)
                     /gene="tcdA"
                     /locus_tag="OO230_000714"
     CDS             complement(17328..18134)
                     /gene="tcdA"
                     /locus_tag="OO230_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5690589.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18372..19469)
                     /gene="mltA"
                     /locus_tag="OO230_000715"
     CDS             complement(18372..19469)
                     /gene="mltA"
                     /locus_tag="OO230_000715"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5690590.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19678..19754
                     /locus_tag="OO230_000716"
     tRNA            19678..19754
                     /locus_tag="OO230_000716"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19712..19714,aa:Met,seq:cat)
     gene            19788..19864
                     /locus_tag="OO230_000717"
     tRNA            19788..19864
                     /locus_tag="OO230_000717"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19822..19824,aa:Met,seq:cat)
     gene            complement(19939..21192)
                     /gene="amiC"
                     /locus_tag="OO230_000718"
     CDS             complement(19939..21192)
                     /gene="amiC"
                     /locus_tag="OO230_000718"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5690591.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21424..22755
                     /gene="argA"
                     /locus_tag="OO230_000719"
     CDS             21424..22755
                     /gene="argA"
                     /locus_tag="OO230_000719"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5690592.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22817..24643)
                     /gene="recD"
                     /locus_tag="OO230_000720"
     CDS             complement(22817..24643)
                     /gene="recD"
                     /locus_tag="OO230_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5690593.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24643..28185)
                     /gene="recB"
                     /locus_tag="OO230_000721"
     CDS             complement(24643..28185)
                     /gene="recB"
                     /locus_tag="OO230_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5690594.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28178..31066)
                     /gene="ptrA"
                     /locus_tag="OO230_000722"
     CDS             complement(28178..31066)
                     /gene="ptrA"
                     /locus_tag="OO230_000722"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5690595.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31242..34610)
                     /gene="recC"
                     /locus_tag="OO230_000723"
     CDS             complement(31242..34610)
                     /gene="recC"
                     /locus_tag="OO230_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5690596.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34623..34973)
                     /locus_tag="OO230_000724"
     CDS             complement(34623..34973)
                     /locus_tag="OO230_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5690597.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34958..35365)
                     /locus_tag="OO230_000725"
     CDS             complement(34958..35365)
                     /locus_tag="OO230_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5690598.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35362..35925)
                     /locus_tag="OO230_000726"
     CDS             complement(35362..35925)
                     /locus_tag="OO230_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5690599.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35916..36386)
                     /locus_tag="OO230_000727"
                     /pseudo
     CDS             complement(35916..36386)
                     /locus_tag="OO230_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36570..37364)
                     /gene="thyA"
                     /locus_tag="OO230_000728"
     CDS             complement(36570..37364)
                     /gene="thyA"
                     /locus_tag="OO230_000728"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5690600.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37371..38246)
                     /gene="lgt"
                     /locus_tag="OO230_000729"
     CDS             complement(37371..38246)
                     /gene="lgt"
                     /locus_tag="OO230_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5690601.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38397..40643)
                     /gene="ptsP"
                     /locus_tag="OO230_000730"
     CDS             complement(38397..40643)
                     /gene="ptsP"
                     /locus_tag="OO230_000730"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5690602.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40656..41186)
                     /gene="rppH"
                     /locus_tag="OO230_000731"
     CDS             complement(40656..41186)
                     /gene="rppH"
                     /locus_tag="OO230_000731"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5690603.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41871..42560
                     /gene="mutH"
                     /locus_tag="OO230_000732"
     CDS             41871..42560
                     /gene="mutH"
                     /locus_tag="OO230_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5690604.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42629..43342
                     /locus_tag="OO230_000733"
     CDS             42629..43342
                     /locus_tag="OO230_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5690605.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43480..43698
                     /gene="ygdR"
                     /locus_tag="OO230_000734"
     CDS             43480..43698
                     /gene="ygdR"
                     /locus_tag="OO230_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5690606.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43806..44846
                     /locus_tag="OO230_000735"
     CDS             43806..44846
                     /locus_tag="OO230_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5690607.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44879..46069)
                     /gene="lplT"
                     /locus_tag="OO230_000736"
     CDS             complement(44879..46069)
                     /gene="lplT"
                     /locus_tag="OO230_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5690608.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46062..48221)
                     /gene="aas"
                     /locus_tag="OO230_000737"
     CDS             complement(46062..48221)
                     /gene="aas"
                     /locus_tag="OO230_000737"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5690609.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48807..49838
                     /gene="galR"
                     /locus_tag="OO230_000738"
     CDS             48807..49838
                     /gene="galR"
                     /locus_tag="OO230_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5690610.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49845..51107)
                     /gene="lysA"
                     /locus_tag="OO230_000739"
     CDS             complement(49845..51107)
                     /gene="lysA"
                     /locus_tag="OO230_000739"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5690611.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51229..52164
                     /gene="lysR"
                     /locus_tag="OO230_000740"
     CDS             51229..52164
                     /gene="lysR"
                     /locus_tag="OO230_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5690612.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52151..52843)
                     /gene="ygeA"
                     /locus_tag="OO230_000741"
     CDS             complement(52151..52843)
                     /gene="ygeA"
                     /locus_tag="OO230_000741"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5690613.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52972..54390)
                     /gene="araE"
                     /locus_tag="OO230_000742"
     CDS             complement(52972..54390)
                     /gene="araE"
                     /locus_tag="OO230_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5690614.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54705..55466)
                     /gene="kduD"
                     /locus_tag="OO230_000743"
     CDS             complement(54705..55466)
                     /gene="kduD"
                     /locus_tag="OO230_000743"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5690615.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55496..56332)
                     /gene="kduI"
                     /locus_tag="OO230_000744"
     CDS             complement(55496..56332)
                     /gene="kduI"
                     /locus_tag="OO230_000744"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5690616.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56619..57800)
                     /locus_tag="OO230_000745"
     CDS             complement(56619..57800)
                     /locus_tag="OO230_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5690617.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58055..59284
                     /locus_tag="OO230_000746"
                     /pseudo
     CDS             58055..59284
                     /locus_tag="OO230_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            59919..60648
                     /locus_tag="OO230_000747"
                     /pseudo
     CDS             59919..60648
                     /locus_tag="OO230_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT        14991 a        16278 c        14784 g        14595 t
ORIGIN      
        1 ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa tgtttcttac
       61 caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg ttgcaacaca
      121 aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat tatcgccatg
      181 attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc cccggaagaa
      241 gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt ttgatttaca
      301 gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg atcaagatca
      361 ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg cggtaaatga
      421 ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc cgggctgaac
      481 tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac gactcaaacc
      541 agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac catgtggatg
      601 ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt
      661 gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat gacgtttttt
      721 gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga agacatcacc
      781 gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct gctctacttc
      841 ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac cgttgaaagc
      901 ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc gctgatcctg
      961 atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa agcgatgagt
     1021 attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct gatcccgcag
     1081 tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac cggaaacggt
     1141 ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa ccactctccg
     1201 atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa
     1261 tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc
     1321 ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga gcagaacatc
     1381 tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg gatggctccg
     1441 attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg cgcacgtgaa
     1501 ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat cgaaatcaac
     1561 aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt tgccaccctg
     1621 aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc gatgatcctg
     1681 ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac
     1741 atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat cttctactct
     1801 ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat
     1861 gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc ttccagttct
     1921 cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat tgcccgtaac
     1981 ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc tctgactggt
     2041 aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct gccggatacc
     2101 gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg tcgcctgatg
     2161 ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa cttccatgca
     2221 gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg cgataaagtc
     2281 gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga agagcatttt
     2341 ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc agccgatctg
     2401 caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa aaacgagctg
     2461 gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga agtcatgcgt
     2521 ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca
     2581 cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac cactgacccg
     2641 atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga gaacgctgct
     2701 ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc ggctgtgctg
     2761 gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg ttacctgctg
     2821 gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg tgccgaagtg
     2881 ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct ggcagaactg
     2941 ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga gcacaacctc
     3001 ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga gcgtaacgcc
     3061 attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac cagcgaaccg
     3121 cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga catgaacgcc
     3181 aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg cgattaatct
     3241 ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc atagcctgtt
     3301 ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg gctgttcatt
     3361 tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag ggttcgccct
     3421 gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag ccaacccacg
     3481 cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag cgtttaccag
     3541 attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg cctgcattac
     3601 gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac gaagccgatg
     3661 acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca acgattgttt
     3721 cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt gattacttcc
     3781 agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa ccgcagcagt
     3841 tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt gttgcgggaa
     3901 tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa gggatatatg
     3961 agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat aaagagatgg
     4021 cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac ggcaatttac
     4081 agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt atccggcatt
     4141 atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct caagcgttgc
     4201 ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca gtgccgaaat
     4261 gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat cacggttgag
     4321 ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac cttccacttt
     4381 cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag cgttatagcg
     4441 catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat aaaacgcgcc
     4501 cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc ccatacccgc
     4561 aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa ccgatcctcg
     4621 cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta gtgcccacgc
     4681 gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg tcgcggcttt
     4741 cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct gcgggatatc
     4801 atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca cgtagttaat
     4861 ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta cactgggttt
     4921 attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt tgttgctgat
     4981 aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga tcaggtaatc
     5041 cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg ttgggttggg
     5101 cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat cggtcacttt
     5161 cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct tctgataacc
     5221 acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc atgccgtttc
     5281 gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa attactcttc
     5341 aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt catcgctcag
     5401 cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca gcacttcaac
     5461 ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa gcccatgatg
     5521 ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag aaagttcgcg
     5581 tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg ccgcaatcat
     5641 gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat gaacggcatg
     5701 attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat ggaaacgcca
     5761 ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa agacaatatg
     5821 cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc catcctgata
     5881 acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca tttccaggca
     5941 agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct ctctgattca
     6001 aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa ttacccacca
     6061 tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac ctagctcaac
     6121 taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat gaccgctctt
     6181 aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg cccgttttcg
     6241 gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt tctgaaacgg
     6301 gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc cagctaataa
     6361 gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca tgagttcatt
     6421 tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc acttttgaaa
     6481 acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg aaacacatca
     6541 atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag aagttacatt
     6601 attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa taaccttaac
     6661 gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca ggctggcctg
     6721 atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg gatattgatg
     6781 aaaaaactca gttataaagc agggattatt accggattat ttttatatgc cttgggcgct
     6841 gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt tggcctattt
     6901 attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt tacggtatta
     6961 gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa ctcgtttggc
     7021 gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc acatcaatcg
     7081 caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca cagcctggta
     7141 ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt cgccctgctg
     7201 atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc caaacaagga
     7261 tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg ggcggtatta
     7321 gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat tcgctacgct
     7381 gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac cggaaccatg
     7441 gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt cgcaccacac
     7501 aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc agccttcgct
     7561 ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc gattcagtac
     7621 ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata cggttcgtcc
     7681 ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg ttttgtcagt
     7741 gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt cgcggtcatc
     7801 tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt tccgaataaa
     7861 gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc ccggttattg
     7921 acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat atggcgaaag
     7981 ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc gtcgagtgtg
     8041 tcatttccga tacctgtatc gcgggtatgg ctgaagcctc tgcttgcgaa gaaaaattca
     8101 gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat ggcagtgaaa
     8161 ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc actgaacgcc
     8221 ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc atcccggcat
     8281 tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct gccgatgttg
     8341 aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg aaaggtaaaa
     8401 gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt gatcacaact
     8461 tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa ctgcgtcgcc
     8521 gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc tgggctgata
     8581 aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat gccgagcaaa
     8641 gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac atgatgcaag
     8701 gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac aacgccatcg
     8761 ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt gacaccgccg
     8821 aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt gtcgtggcga
     8881 ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc accggcaccg
     8941 ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag cgtgtaacgg
     9001 ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac tcaggttctg
     9061 ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg atgaagccgc
     9121 actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa tggtgcccgg
     9181 cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc gaaggcggcg
     9241 tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta ctgcaaatcg
     9301 cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac aaacgcacca
     9361 actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg ccgtttacgg
     9421 atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc atcggccacg
     9481 ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt atgcacaacg
     9541 ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg gatattgaag
     9601 gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa tacattacac
     9661 cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag tggttagccg
     9721 gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa gttatcctgg
     9781 tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag ggaaaaattg
     9841 ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac acctggcacc
     9901 agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca aatcaatagt
     9961 gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt ggatggcgct
    10021 ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg tccgcgaaca
    10081 gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca ggctatttct
    10141 ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa agaaaatcat
    10201 ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat taaccaccgt
    10261 ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat gctggatatc
    10321 cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg ccgactcttc
    10381 cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc cgcagcaatg
    10441 ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca gttcgccctt
    10501 tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg ggaaatttta
    10561 atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg ttccacctgc
    10621 gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc atccggcgtg
    10681 ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat gttgattgaa
    10741 gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga tttattgtcg
    10801 tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca tttctatcgc
    10861 gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct ggaaaaaatc
    10921 gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa cacattgtgg
    10981 aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga tgccgaaacg
    11041 accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa cagcccggaa
    11101 gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac tgaacctgaa
    11161 tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc ccgaactatt
    11221 gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg ctcactttcc
    11281 cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga gcgacttgct
    11341 ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg tgatgatggc
    11401 ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta atgcgctttc
    11461 actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt atgaacgggc
    11521 gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga atattctttt
    11581 aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg ggcggttatc
    11641 gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg acctgctgct
    11701 gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca gtaaagaaac
    11761 cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca atcatggacg
    11821 cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca tcaggctgaa
    11881 aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga ttgaagaagg
    11941 gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc agttgcctga
    12001 catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg gtaaacgcga
    12061 acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct gttacgtcaa
    12121 tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt tttcttgtga
    12181 agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg attacaaatc
    12241 gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta aatttaatcg
    12301 ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt ctgatgagcg
    12361 ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg gagacgacgc
    12421 cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca gatgcggcgg
    12481 acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat ctgagcattt
    12541 atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac ttcttcgtag
    12601 cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc attaaccagc
    12661 cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca taccatccag
    12721 gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca cgtcacctgc
    12781 ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac ccacatgttg
    12841 cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac cgcccacatc
    12901 ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa tgcctcttcc
    12961 agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg aatgcccata
    13021 tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc tggtgcaata
    13081 aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac cccagcatcg
    13141 cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa ctctttgctt
    13201 tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac accctgtaac
    13261 atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag ttcccccacc
    13321 acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat taacttatcg
    13381 ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt cacgcgggca
    13441 aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca ctctttttca
    13501 aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc gctttaaacg
    13561 gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc caaccggtgt
    13621 gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc cgctgaacag
    13681 caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga tgcgacgctg
    13741 cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt cgagaccggt
    13801 ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg caccaaaagc
    13861 gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa aacggctggt
    13921 catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt cttgttcagc
    13981 agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt ctgcctggct
    14041 gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa acgggcaaac
    14101 aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg ccactccctg
    14161 cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc cttgctgaac
    14221 gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc gtgacgcatc
    14281 atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt cgcctgtcag
    14341 cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca cccgtagccc
    14401 cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca gcttatcttc
    14461 ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat taaagctgga
    14521 aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg tcaacgcccc
    14581 cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa atatctcttt
    14641 gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat tacggcggcg
    14701 gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta ctgcggcctg
    14761 ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg cggcatcaaa
    14821 aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac tttgatgtta
    14881 aatgaattta acaattagat cacactgtgt aacctattag tttttttaat ctgagccatt
    14941 ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact tcctgagccg
    15001 gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg gttgtgatgt
    15061 tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag ctaccttttt
    15121 tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc gcctaattgc
    15181 ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat ctctttcacg
    15241 tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat cataccgata
    15301 tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt ggtgtgcatc
    15361 acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc agaaataatt
    15421 gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa tagtattgat
    15481 taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc ggcttcgcca
    15541 ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa ttttgcgtca
    15601 cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc agtttcgcgc
    15661 ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga ccgcgcttaa
    15721 acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg gaaacgtcca
    15781 tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg cacgagagaa
    15841 agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc gcggcaccac
    15901 tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac cgggcgatga
    15961 gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga tggtcgccca
    16021 acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc cggatgtcga
    16081 tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc agatgtcgaa
    16141 cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt cagccgggat
    16201 ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg ttcagcaact
    16261 ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg gtatcggcgt
    16321 gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg gcggcaaaat
    16381 gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga aactggaagc
    16441 aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat ggctggcaga
    16501 ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg cagaagaagc
    16561 gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc tgctggcctt
    16621 tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt acggtattgc
    16681 cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg taaccggcac
    16741 actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc tggtgaatgc
    16801 cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt cgccggacat
    16861 ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc gttgtcgcaa
    16921 tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc tttgccagac
    16981 gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg gctgggatat
    17041 accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg cattgtgcgc
    17101 ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga attacaggca
    17161 cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag cgcctcacgc
    17221 agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca ggtttaagca
    17281 cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta accctgacgc
    17341 gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc aaaggtggcg
    17401 gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg cccttctgcc
    17461 gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc ttcagtagaa
    17521 aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa atcgcttttc
    17581 aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag atcggtaacc
    17641 tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag cgggatttta
    17701 ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat ggcatcaatc
    17761 acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac gaaatcatcc
    17821 accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat gacttccgct
    17881 tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt ggtgacgcac
    17941 acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag cgcttccgcc
    18001 gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc aaacagttgc
    18061 aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca tgcatcacta
    18121 attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc accctaaacg
    18181 cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat attttggatt
    18241 tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac aacgcgcact
    18301 tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa aacggcatac
    18361 agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt cagcacccaa
    18421 acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc gatcccttga
    18481 tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac catcagacgc
    18541 aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc tgccagcaag
    18601 gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc aaccagcggc
    18661 accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac gaaagacggg
    18721 ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca atgacgaatc
    18781 gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac cttaccaatg
    18841 ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg actgccatca
    18901 ccaaagtcga tatacccact accctgcaca tccataatga agttatccat cagagagttg
    18961 ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc acgagacggc
    19021 agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc ctggcgggta
    19081 tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc atagttgtcg
    19141 gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt atcaccgcct
    19201 gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata cagacgcggt
    19261 gacgaattac ggatatggtt aatttgctcg acaaaatcac cggcgttaat cggcgcgcca
    19321 acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc tttatattgc
    19381 tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac cgtgcccata
    19441 agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa aaataaggcg
    19501 caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct gacgcaaatt
    19561 ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta aatccgtgat
    19621 cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat ccacggacgc
    19681 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    19741 ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc ggggtggagc
    19801 agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc ggcccccgca
    19861 accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat aaaaaaagcg
    19921 ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg caaaatacgc
    19981 tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg ttttcagttt
    20041 acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg gaatatctgg
    20101 tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct tgttgatttt
    20161 tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag tcagcgactg
    20221 caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca cgccaccaat
    20281 caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg ttgcgccttt
    20341 ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg cgtcggcatg
    20401 gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca cttgcaacgg
    20461 aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt cctcgatcag
    20521 agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt tgtatttccc
    20581 caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa tcggacgatc
    20641 gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct tttcgaggtc
    20701 gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct gtgcattggc
    20761 cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa gggcaaacag
    20821 ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg ggtcaaattg
    20881 cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg ccatcccctt
    20941 gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag ggttactcaa
    21001 cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg tgtagctgga
    21061 cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga cctgacttac
    21121 gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga ttgcagtgtt
    21181 ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa gtaaacgact
    21241 aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa cggtaaacat
    21301 tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa tttacacttg
    21361 caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc aaaggggtgt
    21421 gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc ggttccctat
    21481 atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc cattgagcat
    21541 gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg catccgtctg
    21601 gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca tcaccacgaa
    21661 ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact ggtgaagcag
    21721 gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct caataacacg
    21781 ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc ccagccgctg
    21841 ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat tgatgaagac
    21901 gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt cgctgtttca
    21961 gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact agccatcaaa
    22021 ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa tgacgacggt
    22081 gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc ccaggaagag
    22141 aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa agcctgccgc
    22201 agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct gttgcaagag
    22261 ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga gcagattcgt
    22321 cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct ggagcagcaa
    22381 ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa attcaccatt
    22441 attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc ggaagagaag
    22501 attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc acggggcgag
    22561 gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa attgtttgtg
    22621 ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt ggatattgat
    22681 ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa agtgttgatg
    22741 gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac tcgtgcgtcg
    22801 catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca ctgcgccgct
    22861 cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc gacagacgac
    22921 gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc gtgcgttggc
    22981 tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt accgtcatcg
    23041 cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata ttgccgtccg
    23101 gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg atatcgccat
    23161 taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct tcgtaccaac
    23221 gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca attcgctcat
    23281 tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg tactcattga
    23341 acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga taacgtccgt
    23401 aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt aaaagtcgtt
    23461 tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta tcaccacggt
    23521 tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag cttttttgca
    23581 gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc ggaacgtgag
    23641 tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg ttggcataag
    23701 cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca cgatcgccga
    23761 gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc ataggcagat
    23821 cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta ccggcatgat
    23881 gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg ctggcatctt
    23941 ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc ttgccgagag
    24001 attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata cggcagcgtt
    24061 cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc gttttaccgg
    24121 tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc gctgcaactt
    24181 tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc gccagtagag
    24241 cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc gtccgctcgt
    24301 tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc gtgggttcat
    24361 ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt agctcaccga
    24421 tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc agtcgtgaaa
    24481 gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc gcgagggtga
    24541 cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc gggcgtagct
    24601 gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct cctccagggt
    24661 cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc gggttgtgta
    24721 aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga taacgccgcc
    24781 aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat gcagcgccag
    24841 ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg ccatagcctg
    24901 ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga gcaggtaata
    24961 acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc gtacctgcat
    25021 gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta gtgcatcaag
    25081 ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca cctgtttatc
    25141 gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct ggaggacagc
    25201 cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga gttccagttt
    25261 ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa acaaactgtg
    25321 caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg tcggttcttc
    25381 aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat cctgagcgat
    25441 accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat cgccgggcaa
    25501 tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct gccagggctg
    25561 gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg cttcaataca
    25621 ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc cgagcgcact
    25681 ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg gtgcaacgcc
    25741 gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca gatcttccgc
    25801 cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat ccaaaactgc
    25861 ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat tggtaataaa
    25921 tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga caatctgcac
    25981 cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt ctggctcgag
    26041 gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg ttccggcttc
    26101 ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc ctgccgttgc
    26161 cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg gcataacgcc
    26221 acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat cccacgcatg
    26281 ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg acgttgccag
    26341 cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca accaaagcat
    26401 ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa cggaagggat
    26461 ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc gcaccagcac
    26521 gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca acgcttcgcc
    26581 ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg ccatggtact
    26641 ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca tcgcaggctg
    26701 cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt tcactggaat
    26761 aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct tgttcacact
    26821 gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg catgaacttc
    26881 actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata tggcctgctt
    26941 cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac ggcgaaaaat
    27001 tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg ggaatcgcgt
    27061 acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat cgagccgact
    27121 taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta ctgtttcgcg
    27181 gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt ctgcaagcag
    27241 ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct tcgtgcgatc
    27301 ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac tgtttgtctc
    27361 ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac ggttaaactt
    27421 gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg cgtcgcgcca
    27481 ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag ccagcgtttc
    27541 atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac gattaatatc
    27601 gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta tttcacgcgg
    27661 cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta gcagagactc
    27721 atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca ggttgagcat
    27781 gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc gttcggctaa
    27841 caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc gttcgtacag
    27901 tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat tgctacggat
    27961 acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca gttcttcaac
    28021 gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc gcaaatagag
    28081 cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc gctcgccctg
    28141 taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt tcactcatca
    28201 ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct tcaggatgca
    28261 catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc atgccttgcg
    28321 gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc agttttatct
    28381 gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa tctttactta
    28441 acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta attaccgcct
    28501 gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct gcggttggga
    28561 aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt tgcaaaagga
    28621 agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca tagcccaatt
    28681 gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc aacagagagc
    28741 tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct gccagtgcgg
    28801 agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg accactacat
    28861 ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca tcgcgtgcca
    28921 gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt cgagcccctg
    28981 attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc aaaattttac
    29041 gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc ataatcgcct
    29101 gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag gacttcgcct
    29161 gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat gcctggaaca
    29221 gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg ttagcgttgg
    29281 tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc gccagccctg
    29341 cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg gctttcggat
    29401 tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg cttggcgcat
    29461 acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat ttcttctctg
    29521 acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa gagagcgcaa
    29581 tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta tcgacctgat
    29641 acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg atataccaga
    29701 tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta gcatcgtacc
    29761 gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc atggtatctg
    29821 ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag tcgatatcca
    29881 gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt aacagattga
    29941 gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta tcagttaaag
    30001 acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag ttggcgctaa
    30061 tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg ctgcgattgc
    30121 caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag ttgttatcga
    30181 tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga atgataatgc
    30241 ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc tctttgttcg
    30301 gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt ttattactgt
    30361 aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct ttcagcgcct
    30421 gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca gaaaactttg
    30481 aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc atcccgtcac
    30541 gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg gcgtatttct
    30601 tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg ggcaaggcgt
    30661 cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca ttgtgactac
    30721 cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt ttcgacccca
    30781 tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga tcttccagcg
    30841 acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca gaaaccagca
    30901 agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct ttatcacttt
    30961 tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat ggtgcccaga
    31021 gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac ggacctcata
    31081 atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc cagaatttaa
    31141 tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac tggtgcgcat
    31201 cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg attaaagcga
    31261 aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt ctctggtgtt
    31321 aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac catcatgttg
    31381 ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc gtcatccagc
    31441 atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact ttcaggtaac
    31501 accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga gaggtaatgc
    31561 aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg tagaaaaagg
    31621 cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca aagttgcatt
    31681 ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg ctgcacctgc
    31741 ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat actctgcccc
    31801 ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca ctgtgtttcc
    31861 cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa gcggcggaac
    31921 aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt gatttgataa
    31981 cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc ttcagtatgg
    32041 aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca gaatcgttgt
    32101 agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac aaattcagaa
    32161 tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct ttgtcgttcg
    32221 cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt aagatgcgct
    32281 tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag ataatgactt
    32341 tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc actgttatcc
    32401 tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat taacgcttcc
    32461 aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg tttctggctc
    32521 atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt cattcccagc
    32581 aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat gttaaccggt
    32641 ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag ttcatcacgc
    32701 aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc gataatcgcc
    32761 tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg caggaagaag
    32821 gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc ctgtgccagc
    32881 ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac cagttctgca
    32941 attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg cgcgctctcc
    33001 atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg tccggtggcg
    33061 gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc ggattcgttg
    33121 acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc cgccagcacc
    33181 ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg cagtgataac
    33241 aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc ggaaatggcg
    33301 taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa cggactgtag
    33361 ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg ggcttcctcc
    33421 agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct atggcaaacg
    33481 tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct acgggaaaac
    33541 tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc agactgaatg
    33601 ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg gctgctctcc
    33661 aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc cagcagcgag
    33721 ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc gctatcgcga
    33781 aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag cagtttcgcc
    33841 agataagcag gatctttaat atcgccccag taataacggc aggggttggt aaacaggaga
    33901 tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg cggtaacgcg
    33961 gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt cgcggactcc
    34021 agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg tccgagttca
    34081 tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc ttctcccagc
    34141 ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata gaccagatac
    34201 tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt gtcgctatcg
    34261 tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg cggcagcaga
    34321 gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg gatttccggt
    34381 aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc aatgtttgcc
    34441 gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc ggtactttgc
    34501 accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac aataaactcc
    34561 atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat agcggctcct
    34621 gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc cccgggtgaa
    34681 actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg gttgacctgc
    34741 cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg ccgccagagc
    34801 ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat acccgataac
    34861 gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag caccatcgca
    34921 accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga cataatgccc
    34981 cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt tttccatcaa
    35041 tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat aaggcttcat
    35101 tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc gagcactgaa
    35161 ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac tgaacgatgg
    35221 cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg cgatcctgct
    35281 gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc agagccagtg
    35341 acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc tggcatccac
    35401 cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca ccggcgagaa
    35461 gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat ccggattagt
    35521 gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg
    35581 ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat cccagatacc
    35641 gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa tttccagccc
    35701 ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg cgacggtaaa
    35761 tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta aactttcacg
    35821 ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga tcgccatagc
    35881 aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc tgtttctcct
    35941 tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc gcccgttgag
    36001 tttttgaagc gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa agcaagcgaa
    36061 ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa tggtgaactg
    36121 ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt cccctccctg
    36181 ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa atagagcaaa
    36241 tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca gtattgccag
    36301 ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag cgtttcaatc
    36361 agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa aaaaagacga
    36421 gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg caggacgttg
    36481 caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc gacgcacact
    36541 ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aacgcccgga
    36601 tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt
    36661 ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt
    36721 tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa atcacccact
    36781 tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac
    36841 ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct
    36901 gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct
    36961 acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag ctggttcagt
    37021 accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg ccactgttta
    37081 ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat ggtgacattg
    37141 ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag ttcgtggatg
    37201 atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg caggttaaaa
    37261 cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg
    37321 ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg
    37381 tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc
    37441 gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt aaactgcgcg
    37501 tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat caggaacaag
    37561 cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc
    37621 accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa
    37681 gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg
    37741 gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg
    37801 ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg tgcgataaaa
    37861 tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata
    37921 acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa cagatacagc
    37981 ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg tcccccgagg
    38041 aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg
    38101 cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaccc caccagatac
    38161 atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc
    38221 ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag
    38281 agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc
    38341 acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat
    38401 aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc aacctgatga
    38461 cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcggca
    38521 aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt catagacaga
    38581 tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg
    38641 cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt
    38701 aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt gcgatcaacg
    38761 gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac cctctttgcc
    38821 agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg
    38881 ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt
    38941 gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag gttgcccgta
    39001 gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc
    39061 gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg catgtaaggc
    39121 agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc attgaacatt
    39181 tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc actttgcagc
    39241 atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc cagtttttct
    39301 tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc gctttttaac
    39361 tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc ttcactaatt
    39421 agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgatag
    39481 ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc gcccatcacg
    39541 gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc gccatctcgc
    39601 acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc tgacagttca
    39661 tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga
    39721 tgaaacagca atcgctgacc cagcgcacgt aaatcaccag cccgctcttt gagatagtta
    39781 tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct
    39841 gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt gtccgaaagc
    39901 aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc ggcaaagcgt
    39961 ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt
    40021 tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca
    40081 tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg gattcgcgtc
    40141 tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc tgccatctgg
    40201 gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc
    40261 tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg
    40321 cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct
    40381 gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg
    40441 agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag gtagtaacaa
    40501 cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg
    40561 tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact
    40621 atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca
    40681 gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct
    40741 ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac cggataccag
    40801 taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct
    40861 gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc
    40921 ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa
    40981 gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc
    41041 gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga
    41101 ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt
    41161 gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat
    41221 gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta cacactaata
    41281 acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg
    41341 tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa
    41401 cccggagcat tctgaataag cccgatatag acattttaaa atattcatta ttagtcaatg
    41461 cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca cacctctcat
    41521 ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc acagaatgtg ccactaagtt
    41581 aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atgaaaacag
    41641 tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt
    41701 ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc gattaccggc
    41761 agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa gttacactgc
    41821 gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac atgtcccaac
    41881 ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa gcacagcaac
    41941 tttctggtta tacattggga gaactggcgg cacttgacgg gctggttacg ccagagaatt
    42001 taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag
    42061 ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg
    42121 atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgcccggtta acgggtaata
    42181 gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta ctgtggatac
    42241 cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca ccgttgctgt
    42301 ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa ttaatggata
    42361 tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat ttacagataa
    42421 gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg ggcgaacgga
    42481 ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca ctactggccc
    42541 gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc
    42601 gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc
    42661 ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg acaatattat
    42721 tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct
    42781 ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct cttgggttac
    42841 gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc gtgatttgat
    42901 tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat
    42961 tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca
    43021 gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc
    43081 agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga tgatgttcgc
    43141 cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc tggcgctctc
    43201 tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa
    43261 aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca acttgattcg
    43321 caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc gtactcactc
    43381 cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc ggtttataaa
    43441 cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg ggcagtaata
    43501 atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg
    43561 accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg
    43621 ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg
    43681 caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt
    43741 tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa
    43801 cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg
    43861 gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg
    43921 ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc
    43981 ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc
    44041 gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaagggca
    44101 tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca
    44161 agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag cgcccgacca
    44221 actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt tcgctgctgg
    44281 atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt
    44341 cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc
    44401 cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc
    44461 tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg
    44521 gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt aatacgctct
    44581 ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg
    44641 atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt gtacgccgtc
    44701 aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg aaaactaaca
    44761 tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa gcggtacatc
    44821 aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc gttaaatatt
    44881 aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc gcaccgaagc
    44941 caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa atgcccagca
    45001 tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc cccgccccga
    45061 cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa aagcccccca
    45121 gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc agggagaaaa
    45181 tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg gtttccagcg
    45241 taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc atcgcgttga
    45301 gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac agcaccaaca
    45361 ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc gaaaaacgcg
    45421 tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc atgttgatga
    45481 gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg gcaacgaccg
    45541 caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag tcagccagca
    45601 caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc attaaaccgt
    45661 tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat ttcgccggtg
    45721 aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg ataccaaagc
    45781 agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc cggcctttgg
    45841 cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca cctacaaaca
    45901 ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt aacgccagag
    45961 tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt
    46021 tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc gtgttgttcc
    46081 gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc
    46141 atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc gtgctcgcgg
    46201 gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag caccagtgcc
    46261 tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg cgaaacacca
    46321 agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt
    46381 tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat atcgccagtg
    46441 tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac ttccagtaca
    46501 cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag ttgcaggcgt
    46561 ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg tagaatacgc
    46621 cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg cgcgcattcg
    46681 gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag ctgtttggta
    46741 ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa gtcatacggg
    46801 ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac ggtgcaactg
    46861 cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata aaggaacact
    46921 tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc
    46981 ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg
    47041 acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc ttcagaaccg
    47101 gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc caggcgcggc
    47161 atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc ttttaaatct
    47221 tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag tttgccttta
    47281 tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat agcactggtc
    47341 agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg cgcgatggcc
    47401 ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat gcgttcgcct
    47461 tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag caatttgcga
    47521 taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc gaagcggtac
    47581 attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat acgcgcttcc
    47641 atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc acgcggcgca
    47701 tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg gaacaagcga
    47761 cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca
    47821 ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta gattttcatc
    47881 agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg gccctgttcc
    47941 accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg aacaaagtcg
    48001 ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta aacggcaaac
    48061 actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga gacgtgatta
    48121 ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac gcgaacgcga
    48181 tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg
    48241 attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc gacagtaaat
    48301 taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag ggtacgaaga
    48361 gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt ctgcacgacg
    48421 actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt
    48481 gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct gcgcaggctg
    48541 gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct gggatcacca
    48601 ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt tcgcaaagtg
    48661 taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc tcacattccc
    48721 acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact gaaacaatgt
    48781 aagcgtttac ccactaaggt attttcatgg cgaccataaa ggatgtagcc cgactggcag
    48841 gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc agcgaagctt
    48901 cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac gccaacgccc
    48961 gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat gtttccgatc
    49021 cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc ggtaatttct
    49081 tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt gagcaactga
    49141 tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccagat gccgatttag
    49201 cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg cctggctttg
    49261 aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg cgtcatttaa
    49321 ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt tctgacgccg
    49381 aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg gccaatgatc
    49441 ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg accgagcttt
    49501 tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg gcggcgggcg
    49561 cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg ttaattggct
    49621 ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg cgttacccaa
    49681 tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat aatcgccctc
    49741 tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca gtgtcaactc
    49801 cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag caattccagc
    49861 gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc attatcaaac
    49921 agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc gccatatgcc
    49981 cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc gcgggtttca
    50041 acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg cccggcgacg
    50101 acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag ggcactgata
    50161 tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc atcaaccagc
    50221 acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa tacgccagac
    50281 tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc caaatggcga
    50341 gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc aaccgcctct
    50401 tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg taaatcctga
    50461 ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg ggcataatca
    50521 acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg ttgtatcacg
    50581 tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc gccaccggta
    50641 ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca tacccgatgc
    50701 cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt caccggaatt
    50761 tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt aaaaacaata
    50821 tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat ttcgcctaac
    50881 gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg aatattggaa
    50941 caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat ctgccgacga
    51001 ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa acgcagcaga
    51061 ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac aaactccaga
    51121 tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc gatttatgtc
    51181 gagtctatgc aaaaatgata tgaattaccg gattgcgaga gagcgctaat ggccgccgtt
    51241 aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct gactgaggca
    51301 gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg ctttgagaag
    51361 gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac cgtgcaagga
    51421 ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat tgtcagcgcc
    51481 gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct gccggtcttt
    51541 tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc cgatgtcagc
    51601 ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc ccagcgtcat
    51661 gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac cgaattactc
    51721 tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa aaaggtatta
    51781 acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga cagctatcgc
    51841 cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat cgtagaaacc
    51901 cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc ggtggttaac
    51961 ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt cagtattgcg
    52021 gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc gctggttcag
    52081 gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct tgacgctatt
    52141 ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc gccgcggtat
    52201 caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct gtgcagccaa
    52261 aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca taataagcgc
    52321 gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga ttaattttcg
    52381 cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc agccgcccgc
    52441 gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga gtcattccgg
    52501 ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga gtgcaacgtg
    52561 actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata ccttctgcgc
    52621 ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta tcccattccc
    52681 cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc atttgcgcag
    52741 agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag taaggaatgg
    52801 tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc tcctgatgtg
    52861 tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc aggtgcccaa
    52921 tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa atcagacgcc
    52981 gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca gcgtgacatt
    53041 tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca gcgcagtgta
    53101 gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg ccgcgccgat
    53161 aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat cgcggcattt
    53221 cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca tcgcataacc
    53281 ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg aagccgtacc
    53341 gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca tcacgctgaa
    53401 accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg caatgaaggt
    53461 cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt ctgtggtcgt
    53521 aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca taccggtaaa
    53581 ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt tacggttgat
    53641 cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt cgttgagttc
    53701 ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg cctcaatatg
    53761 acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca gaataatcag
    53821 cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc tataactgaa
    53881 cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact ggtacatact
    53941 gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag gagcggtgta
    54001 agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca tctctacgct
    54061 ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg ccatcaggct
    54121 gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa ttgctgcgcc
    54181 gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa agtgatcggt
    54241 aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca atcctgcgac
    54301 cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag aacgtggcgt
    54361 taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag taagacattg
    54421 tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata aatatggatt
    54481 aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta agttgaatta
    54541 ttgagattat tattaacctc ctaattttaa agcagataaa attcataaag ttcattaatt
    54601 gataattgat atggattatt tcataaccat gatatggatt atgatgatct agagatataa
    54661 aaaaccctgc catgcggcag ggtcataaaa gtaagaggaa tgaattaacg cgccagccaa
    54721 ccgccatcca cagcaatggt ataaccattt acataatctg aagcgctgga ggcaagaaac
    54781 actaccggcc ccatcaggtc actcggcagt ccccaacggc cagctggaat gcgatcgaga
    54841 atttccgcgc tacgttgttc atctgcccgc agttgttggg tattgttggt cgccatgtaa
    54901 cccggagcta tcgcattaac attaatgttg tgtttagccc attcgtttgc catcaatcgc
    54961 gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat cccgccctgg
    55021 aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc gataaagtgt
    55081 ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat gacatcgtcc
    55141 cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc gttattcacc
    55201 aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg aatgccatca
    55261 atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt gacctgctcg
    55321 atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc ttgcgccagc
    55381 cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc aactttacct
    55441 tggagagaaa atgcacttaa aatcataaca acaccttatt cgtttatgcc cacaactagc
    55501 gcaaatcttt aacgcccaca tggtccatat cattaaagac ctggttttca ccgaccatgc
    55561 cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag ctcggggaga
    55621 tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc cccatcatgt
    55681 ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc cggcgctcgt
    55741 gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc agccccatac
    55801 tcaattgaca ggtttccagt acatccggga caaaatattt gttaatcgtg cgacggttac
    55861 tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact tttttggtgg
    55921 gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt cgccggagtg gcggtatcaa
    55981 tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg cggtgaccga
    56041 tttcatagca ttgaccatcg acagtaatcg taccggcacc gccaatattg ataacaccta
    56101 actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc ccgccaacgg
    56161 aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg tggctgtaaa
    56221 ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg cgcagccctt
    56281 gggtatccag cgtttttgcg tgcgcaccgt ggatgctctg tcttacgtcc acatcaacct
    56341 ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt cgttttctct
    56401 ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac aatgttttat
    56461 ttttaattta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat gagaatggaa
    56521 aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc aacagattaa
    56581 tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg ttcaatggtc
    56641 aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg ggcattacgt
    56701 ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc tatcgggtga
    56761 ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc aagcatcttg
    56821 ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc gacatcagcc
    56881 aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc aattcccatc
    56941 aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac gggtaaattc
    57001 aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc gtttatggat
    57061 gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa acgggctaaa
    57121 ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa cgtctgaccg
    57181 ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc aatcgccgct
    57241 cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct gatgccatat
    57301 tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc atcccacaac
    57361 ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgttcacc ggtgcggcta
    57421 tcagtcagaa catgtggtgc gcggctcatg ttttcctggc cacctgcgat gacaatatca
    57481 gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagacc ggaaccgcaa
    57541 acgtcattaa tcgtgattgc agaaacgctg ttaggaagac cacctttaat agccgattgc
    57601 cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac ttcatccacc
    57661 gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact accaagttcc
    57721 acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg taacgccccg
    57781 acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata gtaaatgatt
    57841 gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa ggaaatatgc
    57901 ctgagcagta gtcagagtca taactggcac gtaaggtttg caaccactaa cccaccaata
    57961 gatgggtaga tagggcgtta atctcccata cttaacttgg tttatggtaa attgccctcc
    58021 attttgttta atttgtagat gatacgttca gataatgtct aatatttggt ctaaagaaga
    58081 aactctgtga agtttcgcgc tctatggcac agccgttgat gcaggcacgc tattccttcc
    58141 tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg tcgcctggcc
    58201 tttaacatat tggccacata aagccttatg ccagttcatc ctctcatcaa aaacatcagc
    58261 aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg gtaatctgat
    58321 taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag tggcaattac
    58381 caactcactt acggaacagc tggcaaagca tatggttatt gatcttcgca tccgtatgtt
    58441 ggtgagtctg ggtgttgtat taattctgaa tctcattttt ctgatgggac gtcatgccac
    58501 tattcgggta atgggatttt tggtattccc attgattgcc tatttcttat ttctttccat
    58561 ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt tcaatcagaa
    58621 tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta gccatacgcc
    58681 cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg ctatggataa
    58741 atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac tgttctttgt
    58801 ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag aagaaggggt
    58861 caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt caatttccgg
    58921 gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact ttggcgttat
    58981 tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa agaaaagtcg
    59041 tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca ttgtttgttg
    59101 cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca ttgccatgat
    59161 acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac cctggcgttc
    59221 catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa tgttctttag
    59281 ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc atttttcata
    59341 atattcattt gttattattc cgccctatca cttctctcgt catatcctag aattatcagt
    59401 tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga tgcataactt
    59461 agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat attctcatac
    59521 catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg aaacataatc
    59581 atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag caaatacaga
    59641 ttaatcgcaa tttttcccag caaaattaca aaatcaaggt gtattgacat catcaatcac
    59701 caatgaaatt atgcaattat atacggatag ggaggttctt aacatggggc tttgtagtcg
    59761 ttataaaagt cttacatgca atagttgctc aatgcattgc caaataatgc cagaagagtc
    59821 accgcgtttg cagtactgcg caaactcgtg ttttgatctt acccagcaat agtggacacg
    59881 cggctaagtg agtaaactct cagtcagagg tgactcacat gacaaaaaca gtatcaacca
    59941 gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag tgaagccctg aagcttgctg
    60001 aacgcatcgg tgttactgcc gcagcccgtg aactcagcct gtatgaatca cagctctaca
    60061 actggcgcag taaacagcaa aatcagcaga cgtcttctga acgtgaactg gagatgtcta
    60121 ccgagattgc acgtctcaaa cgccagctgg cagaacggga tgaagagctg gctatcctcc
    60181 aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag tatgtcttta ttgaaaaaca
    60241 tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc cgggtggccc gcagcggctg
    60301 gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt cagcagttcc gccaacactg
    60361 cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag cgttacggtg ccccacgcct
    60421 gacggatgaa ctgcgtgctc agggttaccc ctttaacgta aaaaccgtgg cggcaagcct
    60481 gcgccgtcag ggactgaggg caaaggcctc ccggaagttc agcccggtca gctaccgcgc
    60541 acacggcctg cctgtgtcag aaaatctgtt ggagcaggat ttttacgcca gtggcccgaa
    60601 ccagaagtgg ccaggagaca tcacgtactt acgtacagat gaaggctg
//
LOCUS       DAJTPG010000011        60419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTPG010000011 DAJTPG010000000
VERSION     DAJTPG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..322
                     /locus_tag="OO230_000748"
     CDS             <1..322
                     /locus_tag="OO230_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5690618.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OO230_000749"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OO230_000749"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5690619.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OO230_000750"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OO230_000750"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5690620.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO230_000751"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO230_000751"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5690621.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OO230_000752"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OO230_000752"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5690622.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OO230_000753"
     CDS             complement(3726..4955)
                     /locus_tag="OO230_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5690623.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OO230_000754"
     CDS             complement(5019..5459)
                     /locus_tag="OO230_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5690624.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OO230_000755"
     CDS             complement(5564..6334)
                     /locus_tag="OO230_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5690625.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OO230_000756"
     CDS             complement(6331..7257)
                     /locus_tag="OO230_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5690626.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OO230_000757"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OO230_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5690627.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO230_000758"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO230_000758"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5690628.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OO230_000759"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OO230_000759"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5690629.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO230_000760"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO230_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5690630.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OO230_000761"
     CDS             12964..13311
                     /locus_tag="OO230_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5690631.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OO230_000762"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OO230_000762"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5690632.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OO230_000763"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OO230_000763"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5690633.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKARYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OO230_000764"
     CDS             complement(15131..15493)
                     /locus_tag="OO230_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5690634.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO230_000765"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO230_000765"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5690635.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKKPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OO230_000766"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OO230_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO230_000767"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO230_000767"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5690636.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO230_000768"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO230_000768"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5690637.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO230_000769"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO230_000769"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5690638.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO230_000770"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO230_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5690639.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OO230_000771"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OO230_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5690640.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO230_000772"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO230_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5690641.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO230_000773"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO230_000773"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5690642.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OO230_000774"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OO230_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5690643.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OO230_000775"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OO230_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5690644.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OO230_000776"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OO230_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OO230_000777"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OO230_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5690645.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO230_000778"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO230_000778"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5690646.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OO230_000779"
     CDS             complement(36348..36386)
                     /locus_tag="OO230_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690647.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OO230_000780"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OO230_000780"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5690648.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OO230_000781"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OO230_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5690649.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OO230_000782"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OO230_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5690650.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO230_000783"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO230_000783"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5690651.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO230_000784"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO230_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5690652.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO230_000785"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO230_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5690653.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO230_000786"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO230_000786"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5690654.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO230_000787"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO230_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5690655.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO230_000788"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO230_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5690656.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO230_000789"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO230_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5690657.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO230_000790"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO230_000790"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5690658.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO230_000791"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO230_000791"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5690659.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO230_000792"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO230_000792"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5690660.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO230_000793"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO230_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5690661.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO230_000794"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO230_000794"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5690662.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO230_000795"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO230_000795"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5690663.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO230_000796"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO230_000796"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5690664.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO230_000797"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO230_000797"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5690665.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO230_000798"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO230_000798"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5690666.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO230_000799"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO230_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5690667.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO230_000800"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO230_000800"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5690668.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO230_000801"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO230_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5690669.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16732 c        14850 g        14365 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgcggtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcggttt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAJTPG010000012        58033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTPG010000012 DAJTPG010000000
VERSION     DAJTPG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(27..281)
                     /locus_tag="OO230_000802"
     CDS             complement(27..281)
                     /locus_tag="OO230_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5690670.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            436..1008
                     /gene="yeiP"
                     /locus_tag="OO230_000803"
     CDS             436..1008
                     /gene="yeiP"
                     /locus_tag="OO230_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5690671.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1231..2697
                     /locus_tag="OO230_000804"
     CDS             1231..2697
                     /locus_tag="OO230_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5690672.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2815..3801
                     /gene="yeiR"
                     /locus_tag="OO230_000805"
     CDS             2815..3801
                     /gene="yeiR"
                     /locus_tag="OO230_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5690673.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3840..4553
                     /gene="lpxT"
                     /locus_tag="OO230_000806"
     CDS             3840..4553
                     /gene="lpxT"
                     /locus_tag="OO230_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5690674.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4965..5531
                     /gene="mepS"
                     /locus_tag="OO230_000807"
     CDS             4965..5531
                     /gene="mepS"
                     /locus_tag="OO230_000807"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5690675.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5712..7268
                     /locus_tag="OO230_000808"
     CDS             5712..7268
                     /locus_tag="OO230_000808"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5690676.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7350..9164
                     /locus_tag="OO230_000809"
     CDS             7350..9164
                     /locus_tag="OO230_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5690677.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9165..10259
                     /locus_tag="OO230_000810"
     CDS             9165..10259
                     /locus_tag="OO230_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5690678.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10259..11284
                     /locus_tag="OO230_000811"
     CDS             10259..11284
                     /locus_tag="OO230_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5690679.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11286..12875
                     /gene="yejF"
                     /locus_tag="OO230_000812"
     CDS             11286..12875
                     /gene="yejF"
                     /locus_tag="OO230_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5690680.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12879..13223)
                     /locus_tag="OO230_000813"
     CDS             complement(12879..13223)
                     /locus_tag="OO230_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690681.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="OO230_000814"
     CDS             complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="OO230_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5690682.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="OO230_000815"
     CDS             complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="OO230_000815"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5690683.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15619..17379
                     /locus_tag="OO230_000816"
     CDS             15619..17379
                     /locus_tag="OO230_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5690684.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17504..17788
                     /gene="rplY"
                     /locus_tag="OO230_000817"
     CDS             17504..17788
                     /gene="rplY"
                     /locus_tag="OO230_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5690685.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="OO230_000818"
     CDS             complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="OO230_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5690686.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19116..19343
                     /locus_tag="OO230_000819"
     CDS             19116..19343
                     /locus_tag="OO230_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5690687.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19363..21123
                     /gene="pbgA"
                     /locus_tag="OO230_000820"
     CDS             19363..21123
                     /gene="pbgA"
                     /locus_tag="OO230_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5690688.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21198..21274
                     /locus_tag="OO230_000821"
     tRNA            21198..21274
                     /locus_tag="OO230_000821"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21232..21234,aa:Pro,seq:ggg)
     gene            complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="OO230_000822"
     CDS             complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="OO230_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5690689.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24288..24935
                     /gene="narP"
                     /locus_tag="OO230_000823"
     CDS             24288..24935
                     /gene="narP"
                     /locus_tag="OO230_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5690690.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="OO230_000824"
     CDS             complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="OO230_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5690691.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="OO230_000825"
     CDS             complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="OO230_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5690692.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="OO230_000826"
     CDS             complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="OO230_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5690693.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="OO230_000827"
     CDS             complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="OO230_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5690694.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="OO230_000828"
     CDS             complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="OO230_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5690695.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="OO230_000829"
     CDS             complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="OO230_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5690696.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="OO230_000830"
     CDS             complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="OO230_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5690697.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30809..31426)
                     /gene="ccmA"
                     /locus_tag="OO230_000831"
     CDS             complement(30809..31426)
                     /gene="ccmA"
                     /locus_tag="OO230_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5690698.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31445..32047)
                     /gene="napC"
                     /locus_tag="OO230_000832"
     CDS             complement(31445..32047)
                     /gene="napC"
                     /locus_tag="OO230_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5690699.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32057..32506)
                     /gene="napB"
                     /locus_tag="OO230_000833"
     CDS             complement(32057..32506)
                     /gene="napB"
                     /locus_tag="OO230_000833"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5690700.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32503..33366)
                     /gene="napH"
                     /locus_tag="OO230_000834"
     CDS             complement(32503..33366)
                     /gene="napH"
                     /locus_tag="OO230_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5690701.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33353..34048)
                     /gene="napG"
                     /locus_tag="OO230_000835"
     CDS             complement(33353..34048)
                     /gene="napG"
                     /locus_tag="OO230_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5690702.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34055..36541)
                     /gene="napA"
                     /locus_tag="OO230_000836"
     CDS             complement(34055..36541)
                     /gene="napA"
                     /locus_tag="OO230_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5690703.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36538..36801)
                     /gene="napD"
                     /locus_tag="OO230_000837"
     CDS             complement(36538..36801)
                     /gene="napD"
                     /locus_tag="OO230_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5690704.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36791..37285)
                     /gene="napF"
                     /locus_tag="OO230_000838"
     CDS             complement(36791..37285)
                     /gene="napF"
                     /locus_tag="OO230_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5690705.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37394..37558
                     /locus_tag="OO230_000839"
     CDS             37394..37558
                     /locus_tag="OO230_000839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690706.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37693..38181
                     /gene="eco"
                     /locus_tag="OO230_000840"
     CDS             37693..38181
                     /gene="eco"
                     /locus_tag="OO230_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5690707.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38303..39334)
                     /locus_tag="OO230_000841"
     CDS             complement(38303..39334)
                     /locus_tag="OO230_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5690708.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39568..39927)
                     /locus_tag="OO230_000842"
                     /pseudo
     CDS             complement(39568..39927)
                     /locus_tag="OO230_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40145..41788)
                     /gene="yojI"
                     /locus_tag="OO230_000843"
     CDS             complement(40145..41788)
                     /gene="yojI"
                     /locus_tag="OO230_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5690709.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41864..42514)
                     /gene="alkB"
                     /locus_tag="OO230_000844"
     CDS             complement(41864..42514)
                     /gene="alkB"
                     /locus_tag="OO230_000844"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5690710.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42514..43578)
                     /gene="ada"
                     /locus_tag="OO230_000845"
     CDS             complement(42514..43578)
                     /gene="ada"
                     /locus_tag="OO230_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5690711.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43652..44707)
                     /gene="apbE"
                     /locus_tag="OO230_000846"
     CDS             complement(43652..44707)
                     /gene="apbE"
                     /locus_tag="OO230_000846"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5690712.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44819..45940)
                     /gene="ompC"
                     /locus_tag="OO230_000847"
     CDS             complement(44819..45940)
                     /gene="ompC"
                     /locus_tag="OO230_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5690713.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46678..49350
                     /gene="rcsD"
                     /locus_tag="OO230_000848"
     CDS             46678..49350
                     /gene="rcsD"
                     /locus_tag="OO230_000848"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5690714.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49367..50017
                     /gene="rcsB"
                     /locus_tag="OO230_000849"
     CDS             49367..50017
                     /gene="rcsB"
                     /locus_tag="OO230_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5690715.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50217..53066)
                     /gene="rcsC"
                     /locus_tag="OO230_000850"
     CDS             complement(50217..53066)
                     /gene="rcsC"
                     /locus_tag="OO230_000850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5690716.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53341..54117)
                     /locus_tag="OO230_000851"
     CDS             complement(53341..54117)
                     /locus_tag="OO230_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5690717.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54122..55771)
                     /locus_tag="OO230_000852"
     CDS             complement(54122..55771)
                     /locus_tag="OO230_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5690718.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55772..56331)
                     /locus_tag="OO230_000853"
                     /pseudo
     CDS             complement(55772..56331)
                     /locus_tag="OO230_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56490..>58033)
                     /locus_tag="OO230_000854"
     CDS             complement(56490..>58033)
                     /locus_tag="OO230_000854"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5690719.1"
                     /translation="TSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNN
                     VRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQ
                     GKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKP
                     AAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQN
                     GQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRN
                     NLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGP
                     GARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLL
                     HRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPL
                     ADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTA
                     AILMV"
BASE COUNT        14194 a        15655 c        14260 g        13924 t
ORIGIN      
        1 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
       61 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
      121 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
      181 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
      241 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
      301 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
      361 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
      421 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
      481 aacggcaaac tgctgctggc taaggatatt gatattcagt cgcccactgc ccgcggtgcc
      541 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
      601 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
      661 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
      721 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
      781 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
      841 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
      901 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
      961 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
     1021 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
     1081 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
     1141 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
     1201 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
     1261 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
     1321 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
     1381 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
     1441 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
     1501 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
     1561 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
     1621 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
     1681 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
     1741 cgccgccgtg agcgcagcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
     1801 aatggccatg tggtgaaaaa cgcggtgctg agaatggcag aaaaacgttc gccagaactc
     1861 gccgggtgga taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
     1921 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
     1981 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
     2041 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
     2101 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
     2161 ttcgcctata tcagtgattg catgcaggat cgcgtatttc gctatgctgc cagaacatta
     2221 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
     2281 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
     2341 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
     2401 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
     2461 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
     2521 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
     2581 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
     2641 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
     2701 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
     2761 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
     2821 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
     2881 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
     2941 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
     3001 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
     3061 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
     3121 gatctgttaa ccgcaccagt ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt
     3181 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
     3241 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
     3301 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
     3361 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
     3421 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
     3481 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
     3541 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
     3601 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
     3661 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
     3721 gagctgattt ccagcagcga agctgactgg aatgccttgc agagcgcgct gttgaagctt
     3781 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
     3841 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggccccgcg ctgtttcttt
     3901 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
     3961 tctttaatca gaaactggtc gaaagtaagg cctttttatg gctggttgca ttgactaaca
     4021 atcgcgcctt cgacggttgt tcactgctgg cgatgggtat gttgatgctg agtttctggc
     4081 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
     4141 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
     4201 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
     4261 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
     4321 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt atggtttttg
     4381 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
     4441 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
     4501 ccttttttga caaatcacta ccaggaaaaa acaaacattt ccaaaacaaa taactcacag
     4561 taattaacat catcagggtt atttttatag tgaggataat cctgatgatg cgcaccgtgc
     4621 tttcatctat cgaacgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
     4681 ctcaattgta ctttaattga ccaaccccgc ttattaactt tctgtatcac tttttcttat
     4741 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt
     4801 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
     4861 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt
     4921 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
     4981 cgattttgag atatatcttg cgcgggattc ccgcgattgc agtagcggtt ctgctttctg
     5041 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
     5101 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
     5161 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
     5221 gcagcactaa aaaaggtatc gattgttctg gtttcgtaca gcgtacattc cgtgagcaat
     5281 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
     5341 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
     5401 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
     5461 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
     5521 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
     5581 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
     5641 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
     5701 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
     5761 ttgtagcagg cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc
     5821 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
     5881 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
     5941 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
     6001 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
     6061 tcaatgaatt gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg
     6121 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
     6181 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
     6241 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
     6301 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
     6361 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
     6421 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctt tgctattacc
     6481 tgatgagcgt acgtatgcgc cccgacagag aaatcatgac cgccatcaag cgcgaacaat
     6541 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgggtaacg ggcctggaag
     6601 tactgctacg ctggcggcat cctgtagcgg gagaaattcc cccggatgcc ttcattaact
     6661 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
     6721 gcgatgccgc agaattagaa aaagtactgc cggtaggcgt caaatttggc attaacattg
     6781 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
     6841 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
     6901 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
     6961 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
     7021 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
     7081 acgcggtgct gacgctggcg aaacgtctca atatgctgac agttactgaa ggggtcgaaa
     7141 cgccggaaca agcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
     7201 ttagtcaccc gttaccgctg gacgattttg ttcgctggct gaagaaaccg tatacgccgc
     7261 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
     7321 aatgatgagt aaaaggaaat ccgttgcaga tgattgtgcg catactgctg ctgtttatcg
     7381 ctctgttcac cttcggtgcg caggcgcagg ctatcaagga aagctatacc tttgccgtgc
     7441 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
     7501 caaaaggtgg gcagataacg ttgtcagccc tcggtacctt cgataatttc aaccgctatg
     7561 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgtta tttacgactt
     7621 ccgatgacga accaggcagt tattacccgc tgattgctga aagtgcacgc tatgctgacg
     7681 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttctccca
     7741 ttactgcccg cgatgtagag tttacttttc aaaaatttct gaccgaaggc gtgccgcaat
     7801 ttcgtctggt ctacaaaggc accaccgtca aagccattgc gccgttaacc gtgcgcattg
     7861 agttagctaa acccggcaaa gaagatatgt tgagtctgtt ttcgctgccg gtatttccag
     7921 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
     7981 gtccgtacca cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
     8041 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
     8101 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
     8161 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
     8221 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
     8281 cgtttaatat ccaacgtgcg gtattcagcg atcgccgggt gcgggaagca atcactctcg
     8341 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
     8401 gttattttca aaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
     8461 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
     8521 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
     8581 acgaagcggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
     8641 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
     8701 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
     8761 ctaaccgcat gcgcaatcgc gactatgaca tgatgccgcg actatggcgg gcgatgccgt
     8821 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
     8881 cccccggcgt gcaaagcccg gttatcgact cactgatcaa ccaaattatt gccgcgcagg
     8941 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
     9001 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
     9061 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
     9121 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
     9181 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
     9241 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
     9301 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
     9361 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagat ccagaagtga
     9421 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
     9481 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
     9541 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
     9601 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
     9661 ttgacgtctg gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg
     9721 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
     9781 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
     9841 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
     9901 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
     9961 aaggggtaag tgagaaaaac attctctgga aacatatgtt ccgcaacgcc atgctgctgg
    10021 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    10081 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    10141 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    10201 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    10261 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    10321 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    10381 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    10441 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    10501 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    10561 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    10621 ctggctggga acggatgcaa acggcggcga tgtgctggca cgcattctct atggcacgcg
    10681 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    10741 gggggcgcta caaggctatt acggcggtaa agtcgatctc tgggggcaac gctttattga
    10801 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    10861 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    10921 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    10981 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    11041 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    11101 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    11161 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    11221 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    11281 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    11341 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    11401 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    11461 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    11521 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    11581 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    11641 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    11701 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    11761 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    11821 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    11881 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    11941 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    12001 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    12061 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    12121 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    12181 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    12241 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    12301 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    12361 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    12421 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    12481 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    12541 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    12601 cattaattct taagccctcg ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    12661 cggtacaggc gcaaatattg acgcttttga aatcattgca acaaaagcat caactggcct
    12721 atttgtttat cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac
    12781 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc
    12841 aggagtatac gcgtcagtta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    12901 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    12961 tggcgattta caaacaggca cggctcacct tcacactcca acaccgaaca tggaacttcg
    13021 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    13081 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    13141 tcaaccttcg aacctgcgaa accagcagac cagcgatagt tctgcggcag tcgatggacg
    13201 attgagagtt gtaatgaagt cacttatttt ccccggaagt acattcactt cacaatttgt
    13261 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    13321 cctcgcctgc gtggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    13381 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    13441 gactttttac ataaattgat tttacataaa ataaacatat atcggggaaa tgatagattt
    13501 gtggttgaca tgcatcaaca aaggaagcct tttaccttcc tcgttgtgca atagatcacc
    13561 gttttttcgg ccgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    13621 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    13681 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    13741 gaaactcatc aagaatgacc gccatcgcat tggatgacac catcgacacg cagcccacaa
    13801 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcggcgca ctgatgacca
    13861 tccacgcagc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    13921 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    13981 caaagtaata accaaagttt tccggcgcga cgtggttaat ttcaatataa acaaacggtc
    14041 cggcgcttaa gaatgagaac atcccggcaa agctgaaacc actggcaagc atgtagctca
    14101 ggacacgttt atggcggaac agcgccgcaa agttaccaat agtggtacga atgtgaaatg
    14161 gctgacgacg ctccggtggt aaggtttctt taatcaggaa gaaaatcatt gccgaagcca
    14221 gaatcgccgc taatgccagg atccagaaga tgtaatgcca gctcagccac accagcaccc
    14281 agccgccaac tatcggtgcc atcagcagtg caatggtcgt caccagcatg acaaacgaca
    14341 tcatccgcga gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc
    14401 tggccgcagc cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt
    14461 gtgccaacgc acacgccacc gcggcggcgg caaacaccag cgtcccgcca agaaccaccg
    14521 gcttacgccc gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca
    14581 gaatataagt actgagggtc atctgcgtac tgcccgccga tacgccaaac tgcgctgaaa
    14641 ttaccggtag cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc
    14701 caaggataaa aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa
    14761 agtcattgaa aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac
    14821 cgggggctaa atcagcatcc agcgtaatac cgccaatacg ttcacgatgc agctcaacca
    14881 cgtggttacc cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg
    14941 tcagacgaac ctgcgttggg gtaatcactt caagcaccgc cggcttagtg agatcttttt
    15001 cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct acaggtgatt
    15061 ccagtgtcac cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc
    15121 actgaccatc atcagtcatc agcaccagac cggtggtatc gatatccaga cgtcctgcgg
    15181 cgtgcagttt ccacgctacc ggttcatcca gaaaatagag caccgtcggg tgatccgggt
    15241 cgtccgtgga gcaaacatag ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt
    15301 gctgcgccag cgggttgcca tcgtaagcga catcatgttc agggagcagt ttgaacgctg
    15361 cattacgaac gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa
    15421 tagcacggct aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt
    15481 tgcctgtaaa aaacggaggt cgggtaatga cccgaaaatc tgaactgttg tctgcccagt
    15541 atagcggtct aataacttcc ctcaagggaa aaagaaccgt ggcatactat tagcagaata
    15601 atctacctac gcgaaaccat gatttttaca ctccgcccat atcagcaaga agccgtggat
    15661 gccacgctca accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggc
    15721 gcaggtaaaa gcctggtgat agcggaactg gcacggctgg cgcgtggtcg cgtactggtg
    15781 ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg
    15841 gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccacggtaa agtggtattt
    15901 ggcagcgttc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg
    15961 attgtcgatg aatgccaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc
    16021 actcacctga ctaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt
    16081 cgattgggca aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag
    16141 aaagcccttt tccgtgactg catttatgag ctgccgctgc gttatatgat taaacacggc
    16201 tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg
    16261 caggcacaga gtaacgggct gttcagcgaa gctgatctca accgtgagct gaaaaaacag
    16321 caacgcatta cgccacatat catcagccag atcatggagt tcgccgaaaa gcgcaaaggg
    16381 gtaatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc
    16441 gaagatgctg cactgattac tggcgacaac cccggcgctg agcgcgatgt gttaattgaa
    16501 gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cagtactgac caccggattt
    16561 gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac
    16621 caacaaattg tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt
    16681 gattatgcgg gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa
    16741 agtgacaacg ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg
    16801 gggaaaacga ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg
    16861 tttgaagatg acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc
    16921 ccgcaatgta acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta
    16981 ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta
    17041 cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc
    17101 acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc
    17161 cagcgtaccg ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct
    17221 ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttactgcg acatccggat
    17281 tttgtcgtcg cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac
    17341 gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat
    17401 aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg
    17461 ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc
    17521 agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt
    17581 cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg atcacgacaa
    17641 agtcatgaac atgcaagcta aagctgaatt ctacagcgaa gttctaacca tcgttgttga
    17701 cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct
    17761 gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg
    17821 gcggggtttt ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc
    17881 agataaggcg ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag
    17941 acgtccggcg ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat
    18001 cggttgctgg atcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca
    18061 agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga
    18121 agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta
    18181 cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct
    18241 cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg
    18301 cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttca
    18361 atccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc
    18421 ctttcaggaa agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga
    18481 tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat
    18541 tttcgttaac gcgcatactg ctcaggttgc tcagaactgc caccagcaaa tactccaccg
    18601 ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta
    18661 attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct
    18721 gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt
    18781 tatttttggc gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg
    18841 gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct
    18901 ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg
    18961 gtattcaacc accgccagtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc
    19021 agcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat
    19081 atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagta
    19141 atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc
    19201 cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca
    19261 ttgccccggc ccaacgccag gcaattgcca actcttttgc cagcgcctta cagtcctcta
    19321 tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag
    19381 cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat
    19441 attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg
    19501 cttgctggtc gcatttattc ctacgtaagc attatcggcc atttcagctt cctagtgttc
    19561 gccacctact tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg
    19621 aggtttttgt ccgtcattct ggcgacggcg ggaatgacgc tattactgat cgatagcgaa
    19681 gtctttactc gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca
    19741 gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta
    19801 ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt
    19861 cgacgcttcg cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg
    19921 gtgtatatct gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg
    19981 ccgctttcgt acccgatgac ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg
    20041 caggagtatc aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgtacagtat
    20101 ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact
    20161 gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct
    20221 gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc
    20281 tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacg
    20341 cctgcggcat taattactgc gcttaatcag caaggctatc agctggggtt attctcatca
    20401 gatggcttta ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc
    20461 gtacgcaccc aatctgacga tcagaccgcc acgcagtgga tcaactggct gggccgctac
    20521 gcacaagaag ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac
    20581 agcaatcagc aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag
    20641 atcaaacgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggtggtgatt
    20701 atcactgccg gtcggggtat tccactgagc gaagaggaag aaacctttga ctggtcccac
    20761 ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat
    20821 gctctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct gcatgtcagc
    20881 acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac
    20941 tgggttactg ctgcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg
    21001 ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa
    21061 aaaccacagt taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac
    21121 tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag
    21181 tattagcagc cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg
    21241 ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccctt
    21301 acggttggtt tttttatatc tgcaattaat tcgataaaca gaccgtgaca catcacagcc
    21361 tgtttatttt ctgttatcag aacgtccaga ccacacccgc ctgagtattc cacggcgatt
    21421 ctacccctgc gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg
    21481 atgaccttat cccagcctga taaacgccac tggttccagc cacatcatta ttaaatttgc
    21541 catcgtcatt aactttcacc tggttagaat cggcgtattc ctgacgcacg gcggctttca
    21601 gccagggttc cagcgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg
    21661 cttcagcgcg taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt
    21721 agtcctgacc atctgtggta aagccggtaa aggccagata gggtctaaca ctccacaatc
    21781 cgtcaaccca acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc
    21841 caaacgctgt tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt
    21901 tcaccacccc atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg
    21961 tatagctatc gatattacct tttccgccgc gatcaaaacc aatatcagaa tgagagtaac
    22021 caaagaacaa gccgcgaatt gtactgcttt cttcacggga gaaacggcta tcgataccga
    22081 gcgtcaggcc cgtcaatgtt tgctcaaaac cagctcccgc atcagtggtc acgttgttgc
    22141 gggtgttaat tgccgaactc cacatcaccg tatcgtaact aacgcctttt acgctaccaa
    22201 gacgctcacg cacggtgtcc agttctgcat caaataccag cggttgtgcg gctgccatgt
    22261 tcagcacatc agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat
    22321 ggttgccatt atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat
    22381 tgcccaacgt aaatgcagca tcaccgccgc ccgttgttac cagtgtaagg ctatctcctg
    22441 ctgccgggct ggcaccggtg tccgtcacga atattttgaa atcacctgtt gcctgaccgg
    22501 tgacgttgag ctgatcgctc tgatgattgg ccatatcggt acgcatataa aaattgccgt
    22561 ttcccgacag ggtattggtg gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg
    22621 aacgagtcac tggctcagcc atcataatca catttgcgcc ggacatcgca aggttggcta
    22681 agttgaggcg agtgttttgg ttcgtcgtag tgatatcggc aatgatggtc catgcgctat
    22741 tttcgagcga aacgtccgct ttgctggtat ccacggcacc atgcgttcta aaagaagcgc
    22801 cctcagaaac cactacctta cccaccaaca tagcgggtgc ataatttatt tgcggcttca
    22861 tgacctcaag aatgccatca ttccctctga ctgacacgtt aaccattgtg ccagcccaga
    22921 cattagcgcg gccattgttg attaccatat tttcagagat ggcttttgag gaatacgtga
    22981 tacttccatt ctggtctgat cgaccgagat catataccgc atttgcctgc actttcgtac
    23041 cagtatcctt tcccagcgat tgcatagtgg catgctcatc aaggatagtg tcaatggcct
    23101 gcgtgtcctc cataacaatg agcccggaac catcatccag ttcataattt tttgacacac
    23161 catcctttat actaaattgg cctttactgt ttgtaccact cacttccagc gcattcgttg
    23221 agacaattaa tttcccgcct gcttttttat caacgccagt tgcagtcccg ccatccataa
    23281 cctccagtaa gccgccacta tccacagtag tattataagc gaagtcattt tcttcaactc
    23341 gcaaacttcc gccgttttcc aacaacatat tattagcaat accattcttg atatcgaact
    23401 gaccgagacg gtttgttccg aatacctcca tggcccgcgt ggttgtttta atagcaccgc
    23461 ctgttttctg atctaccgca agcgcaaatc ccccctcttt aacctccagt agtcctccct
    23521 tttcgatagc ggcaaaaacg gcccccccat ctttttcaac gattaagcgc ccgcctttaa
    23581 tcgtggtatc attcgctggg ccataaacct gtagcacacc attattgagg gtagtctggt
    23641 ttgccgcgcc acccatttga acaatttgat aaccatcatt taatactgag tattctgcgg
    23701 taccattcat ttcaatgtat tgataccctc cgttaatttc agtattacta cttacaccaa
    23761 attcttttat atgctgttct ccgccaggat tgattttcgt attattggcg gtcccataaa
    23821 ccagctggat atctttttcc aggataacac catcaactgt ctcaccatac tcaactgttg
    23881 atgccaatgc tatgggtgaa tataacgctg tagccaccag aacagatatt gcggaacgac
    23941 aaagagaaac agaaccagat tgatgcattg agctttcatc ctatgaaatt aattgcagtt
    24001 aaaagcattg ggtacagaaa atacccatag ctccataccc ggagtcagtt tttaaaaact
    24061 gtttaaagaa atgcacaagt attgtgattg attttttagt tgtttttctt gatgagaagc
    24121 tgatgcaaaa ttccgtcttt ataataaaaa tgatgccaaa gcgaacgaca aggttgtagt
    24181 tttcactaca tgtccataca taaaatgggg taacattcac gcgcctggta gcgttaccaa
    24241 cgctacgctc aaacataatg attctaataa aacctcagga gactactatg cctgaagcaa
    24301 cgccttttca ggtgatgatt gtggatgatc atccacttat gcgacgcggt gttcgtcagt
    24361 tactggagct tgatcctggc tttgaagtgg tcgccgaagc gggcgaaggc gcgagcgcga
    24421 tcgatctggc gaatagactg gatatcgacg tgatcttgct ggatctcaat atgaaaggta
    24481 tgagtggcct ggataccctc aatgccttgc gcagggatgg cgttaccgcg caaattatta
    24541 tcctgaccgt atccgatgcc tccagcgatg tctttgcgct gatagacgca ggcgcagacg
    24601 gttatctgtt gaaagacagc gacccggaag tattgctgga agcgattcgt accggagcga
    24661 aaggcagcaa agtctttagc gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg
    24721 gcgcggaaga agatcccttc agcgtgctga cggagcgcga gctggatgtt ctgcacgagc
    24781 tggcacaggg gctgtcaaat aaacagattg catcggtgtt gaatatttcc gagcagacag
    24841 taaaagtaca tattcgcaat ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca
    24901 ccattctgtt cctgcaacaa cgcggggcac aataaaaata gcccgatgga tttatcatcg
    24961 ggctgagatt tatgacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg
    25021 gatcgatcaa tttattgaat ttacacaatt ttgtaggccg gataaggcgt tcacgccgca
    25081 tccggcataa actaagcgca ctttgtcatc agtctagccc gatggcatca ccatcgggcc
    25141 tctttttatt tactctcctg cggcgacaaa tgttgcatcg cctgcgcgat actacgttca
    25201 atcaccgcac ggcgagtatc gttggcaggt aagagtttca acatcatctc ccacgcggca
    25261 acggcttcgc caaatcgctg ctgctcaaag gcattaaacg catacatgct tagcacacgg
    25321 atattgctat ggtccgttct caccagctga cgtagcagtt caccgccgag gcggttgtcg
    25381 ttgggatcag atgaacgagt caacgcttca gcgtatccca gtgcggcatc gctgttcttc
    25441 ggatcgaggc gatacgcagt agcgtatgca tcggtggcga tactggcgtt acccagcgcc
    25501 atgccaacgc ggcccaacat aatccagcct tctatatctc ccggattttt ttgcagttga
    25561 gtacgcatcc ccagcgcaag gcgcgacatc tcttcttcgt tgagcggatc ggctttcggg
    25621 tccagcgccc tgtccagcaa cgccggagcc tgtgccgtgg cctgctgcca gattttcacc
    25681 tgctgataat tgccagtctg gtagtagctg acgccagcca caattaacgc caccacaata
    25741 cccggcagat aaacaacata tccggcacgc ttaccttccg gcacgctttg ttcaggaaac
    25801 gcttccggca ccacgcgtac ccgacgccgc gagcgggcgt atatgaccca accgccaatg
    25861 ccaatagcca ctactggcag cacccacagc agcacggtca gcggcgttaa cggcggatcg
    25921 taagtgacga agttgccgta acgcgccacc atataatcga caatctcttt tttactttta
    25981 ccttcctgca tcagttcata cactttctga cgcaggtcgg tggcaatcat cgagttggaa
    26041 tcggcaatgc tgttgttctg gcatttcggg cagcgcagtt cttcagtgag ctgacggaac
    26101 tgttgttcct gtgcttcatc tttaaactgc aacacgtcga tggtcgccag cgctgagccg
    26161 gagatcatca gcatcagcac gcccaataaa aacctcattg tgcggcctcc ttactgtatt
    26221 tctcccacag cggcttgatc tcttcttccc agacgcgagg attgagatcg cccgcatggc
    26281 gatagcgaat gataccgttg ccgtcaataa ggaaggtttc tggcgcgcca tagacaccga
    26341 gatccagccc taacatgccg tcgccatcaa acaggcttag cgcgtaagga ttgcccagct
    26401 ctttcagcca gctgattgcc ttctggcgat cgtctttata gttcatgccg accacgcgaa
    26461 tgccctgcgc tgaaagctga ttcagatatt gatgttccgc acggcaggtc ggacaccaag
    26521 tcgcccagac gttaagcagt actggtttgc cctgagtcag cacatccgcc tgataaaact
    26581 gccccggatt gtccagtgat tcgagacgaa acttcggcac aggcttgcca atgagcgccg
    26641 attccagatt ggtcggatca tccccttcgg cattacgcgc cagctgccac agcagcgccg
    26701 cggcaatcgc caggaagata atcaacggaa ttaacaatac tttgcgcttc atacggcctc
    26761 cggcgcagtt ttttgcggac tcacgcgctt acgatagcga ggatcaaaca gacacagcag
    26821 tccgcccaac gccatcatca gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta
    26881 aagacgcacg gcccacgcgc cgttttccag ctcttcaccg agcgcggcgt acaggtcacg
    26941 cgtgatgccg ccgtcaattg ccgcttcggt catcatcgac ccggcagtgt tgtaataacg
    27001 tttttccgca tacagcaccg tttccggttt accctctcgc gttacgccaa tagtcgccac
    27061 accgccgcgc cagttcgggc cagtcacctc tttgacatca cggaaggtga agcgatattc
    27121 atgaatatcg acgctatcgc cggacttcat gcgcacatca cgctcaacgc tataattctg
    27181 gctaaaggca atgccaacaa ttgtcactgc cagccccagg tgagccgcca ccatccccca
    27241 ataactgaag gtggttttcg tgccgcgtga aatacgcagc gcagcttccg cagttgccag
    27301 caccgcaatc cagcaggcca ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc
    27361 gaacagccac ggcaacagca gagacagcac cagcgtagag atgaaggcga taatcaataa
    27421 attgcggatt ttacgtgggc gatcgcgccc ccagcgcacc agaggaccga caccaagcag
    27481 tagcgcaaac ggcaccatca gccaggtaaa catggtgttg aagaacggtt cgccaatcga
    27541 aatactgccc agtccaagtt gcttgtgcac cagcggcagc agcgtcccca gcaacaccac
    27601 cagcatcgcg gcgaccagca aaacgttgtt cgctaacagc aaagattccc gcgaccacag
    27661 cgcattgttt acgcgtgagc gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc
    27721 gccaatcacc agcaccataa aggcgaggat aaacataccg cgcgccggat cagacgcaaa
    27781 cgcgtgtacc gataccagca cgccggaacg cacgaggaaa gtccccaaca gacacaacga
    27841 gaaggcactg attgccagca gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac
    27901 cgccagtgag tgcatcagtg cagtccccac cagccacggc ataaacgagg cgttttctac
    27961 cggatcccag aaccaccagc caccccagcc gagttcgtaa taggcccatg cggaaccgag
    28021 cacaatgccg agcgttagga aaatccacgc cgccagcgtc cacgggcgag taaaacgcgc
    28081 ataagtgctg tccagacgcc cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga
    28141 gaaacccacg taccccatat aaagcagagg cggatggaag atcagccccg gatcctgtaa
    28201 cagcgggtta agatcgcgcc cttcaatcgg gaagttcggc agcgtgcgtg agaacgggtt
    28261 agaggtaaag agaatgaaca gcaaaaagcc gacgctgacc atccccatta tcgccagtac
    28321 acgggccaca atatccagcg gaatacgctg actaaaaatc gccaccgcaa aggtccagcc
    28381 gctcatcagc agcacccaca gcagaagcga accttcatgc gcgccccaag tagccgccac
    28441 gcgataccac accggaagct gggtattgga gttgctggca acataggtga cggtaaagtc
    28501 gttgaccacg aaagcattga ccagcaccag aaatgcgcca gccacagaca taaacagcag
    28561 ccaggcaaac aagcgggaag acgccatcat gcgcgcatct ccgcgcgcca cgccccatag
    28621 cggatacacg gacagcagca gcgcaatccc taacgccaag cacagcagcc cgtttccaat
    28681 ttccggcatc atgatgctgg gtccttataa acactcgccg ggcgacggtg gttagcttcc
    28741 atcgctttct caacttctgg cggcgtgtag ttttcgtcgt gtttcgccag cacttctttc
    28801 gcgaggatat gattgccttt ttccagctcg ccctgcacca caacgccctg cccttcacgg
    28861 aacagatccg gcaaaatgcc ttcgtaagag acatccactg agccttcagc atcgtaaatg
    28921 gtgaaggtca ctttcagcga attgggatcg cgctgcacgc tacccggcat caccatcccg
    28981 ccaacgcgca gacgctgacc gacttccggc atctgctgag tttcacgctt gccgtagaga
    29041 atttcccccg gcgtataaaa gagatcgata ttcgagcgca gcgcatatag caccagaccg
    29101 atagtcagcg ctagccctgc caacacggca caggcaatcc acaagcggtt tttacggcga
    29161 atattcatgc agcctcctgc tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt
    29221 gcgccacacc acgcagaatt gcgcgatgtt gcatcaccga gtgcacgacc aaaaccacca
    29281 gcggaataac ggtcatcacc accgccagcc agacaaaaaa ggcgtaaccg cccattgcga
    29341 aaaattcatt ccaggaagca aatgcagggg tcatttacgg cctcttttca gtatcagttc
    29401 actcacccac ggacggcgtt tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt
    29461 cagcgtggca gacaggagca ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc
    29521 cggatcgata ctttgctgca tccgcgttga tccctgatgc agggtgttcc accactccac
    29581 ggagtaatga ataatcggca gattcaccac gccaatcagc accaggatac ctgccgcacg
    29641 gcccgccaga cggcggtcgt cgaaggcgtg ccacagggca atcacaccca catacaaaaa
    29701 cagcagcacc agttcagaag tcagacgtgc atcccatacc caccaggtgc cccacatcgg
    29761 ttttccccat gcagagccgg taaccagggc aataaaggta aacacggcac caatgggggc
    29821 catcgccgcc accgccaggt tggccatttt catctgccag acaaggccaa taaacgccgc
    29881 caccgccatt gatgcataaa tgcccatcga ccagatcgcc gcaggcacat gcaggtagat
    29941 aatgcggtag ctatttccct gctgataatc agccggagca aagccgaatc cccagatcca
    30001 gccgacggta agcacgacca cactggcaat tgccagccac ggtataaacc agccacagat
    30061 ttgatacagc cgtggtggga tcgccagttg atgcagtgtt ttccacatag tttcgatacc
    30121 agactcgaac aaaaatcagt aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc
    30181 gtcgcaaaag gacttaatgt cgcggtgcct gccagcaacg cgcctaaaat tgccagatac
    30241 ccatcaacgg gcaaatgcat agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat
    30301 gggatagtca gcggtaacac cagtatgctg agcagcacac caccgcgctt aagtcccact
    30361 gtcagcgcca cgcccggtgc accgagaaag ccaagcgtag gcgttcccag cagcagcgtc
    30421 agcgccatca cttgccagcc ataaacatcc attcccagta gcattgctac cagtggcgaa
    30481 aggatgagta acggcagacc ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg
    30541 gcgggcaagg gtaacggcaa caacatcaat tgttcaagac tgccgtcctg caaatcgtca
    30601 cggaacagtc gttccagcgc cagcaaggat gaaagcagcg cagcaaccca gataatgccc
    30661 ggtgcaatac gcgccagcag ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt
    30721 acaatcagga agaaccacag cgggttggcg atttcggcgc tatggcgaaa cgctacgcgc
    30781 agttcaagac ggaaaatacg ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc
    30841 ggcgaatttt actttcagca acgttgagcg gctggtgggt agtcagaatc acaatccccc
    30901 cctgctccgt atgctgcgcc atacgctggg tcagacgatc gacgccgtta acgtcaatcg
    30961 cggtaaaagg ctcgtcgagg atccataatg tggcacgggt caaccacaga cgcgctaaag
    31021 cgacgcggcg ttgttgcccg gcagaaagtt gatttaccgg aatatcttcg aatccggcaa
    31081 gcccggcctg cgccaaggct tccagacatt gtgcggcatc gccatcgcga tgataaaagt
    31141 gcagattttc taacgccgtc agccgggttt tgatccccgg ctgatggcct atccataaca
    31201 gggtttgatg gtagctgtcg cgtacctgat gcaagggctg cccttgccag agaacctcgc
    31261 cagcgtcagg gcgagacaac cccgtcagca aacggagaag cgttgtcttc cccgcgccgt
    31321 tgctaccggt gatttgtacc cactctcctg cgttcagcgt aaatgacaag ccactaaata
    31381 aggttcgttc atcccgctca caaagtaact ctctggcttc aagcataccc acgcaataac
    31441 cctgttaaaa acctggctcg acttcacgca tatcgggcag cttgtgcgcg atccctttat
    31501 ggcaatcaat acaggtttgc ccatctttca ccgcctggtc atgcatcttc gcggcaaccg
    31561 atttctgggc ggttgtatcc atatactcga agttgtgaca gttacggcac tcctgcgagt
    31621 tattgtcctt catgcgccgc cactcattct gtgccatcgt cagacgatga gcttcaaatt
    31681 tctgcggcgt gtcaataacg ccaaaaattt taccatacag ctctttactt gctttgagct
    31741 tgcgtatcat cttcggcaca aactcgtgcg gaacgtgaca atccggacag gtcgcacgga
    31801 cgccgctacg gttgttgtag tgcacggaat ccatgtattc ctgatacacc gtgttgcgca
    31861 tttcgtggca gctaatgcag aactcttcgg tattggcttt ttccatcccg gtgttaaagc
    31921 ccccccagaa gacgatgccg ccaacaaaac cgatcaacag cagcgtcccc agcgccagat
    31981 ggctgggggt acgccaccat ttccacaggc gcttaatcag accaggctta cggtcagaat
    32041 ttcccataat aacctcttat ttcccgtaac cttttgatgg ggtaaaggta ttccccacga
    32101 ttggcgcggt atcggcctgc ggtacgtgac attgcagaca gaaataacga cgcggagcca
    32161 cttccgcgcc cactttgccg tcgctgtcca taaagtgagt aggactgata cgcggcgcgc
    32221 cagtggtgcg atagctttcg acaccgtggc actgcaagca gcgattggta ttggtcgtta
    32281 cctgataacc ttcaacgcta tgcgggatca ttggcggctg attcacatag ttcagcggca
    32341 tccgatcctg ctcttttggc atccgaatgg ccccttcctg cgtcccggat acttccggcg
    32401 actggctaaa atcgacgcca ttagccgccc aaaccgcgcc gcttaccacc agggccagca
    32461 tcgccgtcca ttgacacagc gctttcttca ggtcatggct tttcatgatt tcgctcccga
    32521 actccatcgt gtagttattg taaatacatc ctcagaacag acatccacgc agcgaccgca
    32581 cgtcatgcaa tcgcggctgg tgacctgcac cgggctttgt tcatccagca ccggggcacg
    32641 tagcacatgc ggttccgggc aaacatgaaa acaatccata cagcggttac atttctgccg
    32701 atcgctggcc gcaacggtaa tcacaccttt gctacccagc acgccataca gcgcgccaac
    32761 cggacaaatg tgcccgcacc agccgtgttc aacgaccagt agatcaaata aaaacagcgc
    32821 gagaataagc agcgcgccgc tgccgaagcc cattaccagg ctacgcccca tcagagaaac
    32881 ggggttgatc cattcccaaa tgagcgtgcc agttaaggct gatcccacca gaatgaccac
    32941 caacagcacg tagcgaatgt ggcgagggat cgtcgcagac tgattgaggt caaagcgcct
    33001 gcgtaaccag ttcgctaagt cggtaatcgg gttcagcggg cagacccagc tgcaaaataa
    33061 tcgctttccc gccagggcgt agagcacggt gataatcacc gcaccggtca acgccaccgt
    33121 ggcaggcaga tgaccactgg caagactttg cagcgtcatc agcggatcgg tcagcggaac
    33181 ggtgtcgaat aacaggctac tgctgtagtt gccgtgcaag atccacacac caaaccatgg
    33241 accgctcaaa aacatcccca gcacgaagaa ctgacaaagg cgacgtaaca ccagccaacg
    33301 gtgactgcgc caccagcctt ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt
    33361 tgccattgtt cccctccagc cagccgaagc ggtaatggtg acctaactcc cctttcgcca
    33421 gtgacagcgg taacaccttg attgccggtt gttccagcac gcatactttt tcgcacttac
    33481 cgcaaccggt acaggcgtcg ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggtac
    33541 gcgtgttgcg ctccagctcc aaggtgatgg cctcatcaat tttcgggcat tcgcgataac
    33601 aaacatcgca gcgcagcccc tgaaagttga gacagttttc ctggtccacc agtaccgcca
    33661 gccccatccg cgcgtcgtcg atcgattcaa tctcacgatc cagcgcaccg cttgggcaca
    33721 ctttggcgca cggaatgtcc tcacacattt cgcaaggaat atcccgtgcg acaaaatatg
    33781 gcgtgcccgc cgacagacca gaggccagcg tcgccagttt taaggtgtcg taagggcaag
    33841 cctgaacaca ctgaccgcaa cgcacacagg cactggcaaa ggcgttctcg tttatggctc
    33901 cgggcgggcg caaccgcacg ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca
    33961 cacccacggc agccagcccg cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac
    34021 cattttgagg tttcgctgac cgggacataa tggcttacac cttctccagt ttgaccgcgc
    34081 acttcttgaa gtccgtctct ttcgagagcg gatcggtcgc atccagcgtc agtttgttaa
    34141 ccagctgtgc ggcgtcgaag aacggcatgt acaccaggcc ctgtggcgga cggttacgac
    34201 cgcgcgtttc aacaatcgag atcacttcgc cacggcgaga aaccactttc actttgtcgc
    34261 cacggcgcag atcgcgcgct ttcgcatcca gcgggtgaat aaacaagacc gcttccggga
    34321 aggcgcggtg cagttccggc acacggcgag tcatactgcc ggtgtgccag tgctccagaa
    34381 cgcgtccggt agagagccac aggtcgtact cttcatccgg tgcttccgcc gccggttcga
    34441 acggcagtgc gaagatcacc gctttgccat ccggtttacc gtagaactta tagccttcgc
    34501 ccgctttcac gtacgggtcg ttaccttcgc tgtaacgcca ctgtgtttct ttaccattca
    34561 ccaccggcca gcgcagaccg cgcgctttgt ggtagtcatc gaacggtgcc agatcgtgac
    34621 cgtgaccgcg accaaaccat gcgtactctt cgaacagccc tttttgcaga tagaagccca
    34681 gctcgcggga ttcatcgttc agctgatctt ccgccagttc ggataccggg aatttgctca
    34741 cttcgggggt ggcatacaga acttcgtaca gcgttttgcc acgcagttcc ggtttcttcg
    34801 ccagcagctc ttccggccat acttcttcag ttttgaagcg gcgggagaac tggactaact
    34861 gccagagatc cgatttcgct tcgcctggcg cctgtacctg ctgacgccag aactgagtac
    34921 ggcgttcggc gttaccgtaa gcgccctctt tctctaccca cattgcggtc ggcaggatca
    34981 agtcggcggc cagcgcactg actgtcggat acggatcgga gacgatgatg aagttgcgcg
    35041 gatcgcgcca gcccggcata cgctcttcat tgatgttcgg cccggcctgc atgttgttgg
    35101 tacacatggt ccagtaaaca ttcagcttgc cgtctttcag cgcacggtct tgtgccaccg
    35161 catgaagacc gattttcgcc ggaatggtgc cgctcgggat attccacttc ttctcacaga
    35221 tatcacgatg tttctcgtta gttaccacca tgtccgcagg cagacggtga gcaaaggtgc
    35281 ccacttcacg cgcagtacca cacgcagaag gttgcccggt cagggagaac ggaccgcaac
    35341 ccggctggga aattttgccg gtcagcaggt gcaggttgta gaccaggttg ttagcccaca
    35401 caccacgggt atgctggttg aagcccatcg tccagtagga gatgactttc ttgttcggat
    35461 cggcatacag ctgcgccagt tgttctaact ggtctttcgg cacgccggtc atttcagcgg
    35521 ttttttccag cgtatactca gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg
    35581 aggcgtcaga acccggattc ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt
    35641 agccgatgtc cgtcgcccct ttgcgcaggt taacgtgctt gctgaagaag tcctgattta
    35701 tcgcattgtt ttgaatgata tagttggcga tgtagttcag gatcaccagg tcagattgcg
    35761 gcgtaaagat gatgccgtta tccgccagct cgaagctacg atgctggtag gtagaaagca
    35821 ccgccacggt gacgttctgg ttagagagac gactgttagt gatgcgtgac cagaggatcg
    35881 ggtgcatctc cgccatgtta gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt
    35941 catagcagcc catcggctca tccataccaa aggtacgcat aaagccaact actgccgacg
    36001 ccatacagtg acgtgcgttc gggtcgatgt tgttcgaacg gaagcccgct ttgaacagct
    36061 tggacgcggc ataaccttcc cagatagtcc actgaccaga accgaacata ccgatcgatt
    36121 ccggcccttt ttctttcagg gcggttttga atttctcttc catcacatcg aaggcctgat
    36181 cccaggtgat tggggtaaat tcgccttctt tgtcatattt accgtttttc atacgcagca
    36241 gcggctgcgt caaacggtct ttaccgtaca tgatcttggg caggaaatag cccttaatgc
    36301 agttcaggcc acggttaacc ggtgcgtccg ggtcgccctg acaggccacc acacgcccct
    36361 gctgcgttcc gaccagaacg ccgcaaccag taccgcagaa acggcacggc gctttatccc
    36421 atttaatggc ttcctgctga ccaacaacgg cgcgggcaac gcccggcacg ctgagaccgg
    36481 cagccgccgc agcggccgca acggcgttag ctttcataaa gctacgacga ctgagtttca
    36541 tggtgtttcc tcaccttgct cttcctgctg gtgataaacc agcgacaccg ccagcacgcc
    36601 ctctacgttg cgtactgact caatggtttg gatcagcgtt tcgctgtctt ctgcttccac
    36661 caccacaatc aactgaccgc tcggcgcgtc gctgacagca acttcacagc cgggaaaggc
    36721 gttcagttgg gtgctgatgt ctgaaattcg ttcgcttttg gcctgcacga ccaggctgca
    36781 aacttgccag ttagtgtgca tggagatact ccgcagttat ggctgatacc ggacagctgg
    36841 ctgcacatgc tccacatccg ttgcaaagtt ggctattaag ttgcgcctgg tagatcccgg
    36901 agagcgtcgg gcgaaagata attgccattg gctcacagct atcctgacag cggcggcatt
    36961 caactgactg atacgccagg cacgcgtccc cgatggtgaa ctgcaaatcc caggccctgg
    37021 tgtggcgcgg agaaaatagc gattcggggc aagcctgcgc gcaggcgtaa cagaagctgc
    37081 actcgttatt tttgaaatta acgctcggat agccacccgt gccgcgttgc agaatgttgt
    37141 tttcacaagc attaatacag gcgtcgcagc gggtgcaatg ggtcagaaaa tgagattcat
    37201 caccggacca gggcggacgg ataccgttac tggctttgcg ccagcaacca gtgagtatgc
    37261 cccgacggga tgcatcaatc ttcacattga ccttccatca ttaacgcgct ctgaaaattg
    37321 agagcgacca aatagaccgc ataattaata agccattttt atagccgcta agatattaaa
    37381 ggatgtgtca aagatgcata ccccgatcgg ggtaaaacct gtagcaggat caaaagagtg
    37441 gcgggaagcg tggcaaaaac gggcttttgc tcacatttca aatggttata aacatattta
    37501 tatagcgatt aattcaccag agatatttct gctggtttgc tccctcatca gaatttaaca
    37561 ctaaaagagc aggtaaaatt gtctgaatgt tctttaagtt attcataaag caaattaata
    37621 aatctgatga atatgttaac cttcagcgac atcatcggtg aaaacctata aatgaagaag
    37681 gaaagcaaaa aaatgaagac cattctacct gaagtattgt ttgccgcttt cgctaccact
    37741 tccgcctggg cggcagaaag cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct
    37801 gaaacaggca tgaagcgtca ggtgattcag ttaaccccgc aagaagatga atctaccctg
    37861 aaagtagaac tgttaatcgg tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc
    37921 gggaagctgg aaagcaaaac gctggaaggc tggggctatg attattatgt ctttgataaa
    37981 gttagctcgc cggtttccac gatgatggcc tgcccggatg gcaagaaaga gaagaaattt
    38041 gtcaccgcgt atctgggcga tgctggaatg ctgcgttaca acagcaagct gccgatcgtg
    38101 gtgtatacgc cagacaatgt agatgtgaaa taccgcgtct ggaaggcgga agagaaaatt
    38161 gacaacgcgg aagttcgcta aactgttgtg aagtacggca ccccgttggt tggataaggc
    38221 ggttacgccg catccaacat ctaacgcccg agccggttgc ctgatgcgac gctggcgcgt
    38281 cttatcagta tacgccacat cattacaacg caatatccgc cacttctttt tgaacgggtt
    38341 gcggtttcaa ctgcggtttc ggcgtactat ctgctgcttg cggtttgtcg tatttcagcc
    38401 ccagcacttc gctggtgtac tgcaactcgc gttctgttgc cgctacatca ccgttcagct
    38461 tgcgtccata agacggaacg atcgctttca acgtagcctg ccattgcggg ctggaaacgc
    38521 gatcgccaaa tactttttcc agtagattca acataatcgg cgcggcggtt gacgcccctg
    38581 gcgatgcccc cagcagcgcc gcaatggtcc cttgctggtc actgaccact tcggtaccca
    38641 gacgcagtac gccacctttc tcggcatcac gcttgataat ctgcacacgc tgccccgctt
    38701 gccacaaacg ccagtcctct tttttcgctt gcggatagta ctctttcaac gcttcaaaac
    38761 gatcctcttc actcaacatc acctgactca ccagatattt caccagatcg aaattatcca
    38821 gcccgacgtg catcatcggc atcacgttag aggtggtggt ggaactcatc agatcccaca
    38881 atgaaccgtt tttgaggaat ttggttgaga aggtggcaaa tggcccaaac agcactacgc
    38941 gtttaccgtc cagaacacgg gtatcgatat gcggaaccga catcggtggt gcgccaacgg
    39001 atgctttacc gtaaactttc gccagatggc gattaaccac gtctggattt tccgaaacaa
    39061 ggaactgtcc gcccaccggg aaaccggcgt agtctttcgc ttccggaatc cccgattcct
    39121 gtaacagctt cagcgccgca ccgcccgcgc cgataaagac aaatttggcg cggatgttct
    39181 gtgcagtgcc gtttttcaga tcggcaacgg taacggtcca ggtgttatcg tcattacgct
    39241 ttagggcgcg gacttcgctg ctgagttgca gcgagaagtt agatttcttc tgtaaggaag
    39301 caattaactg gcgggtgatc tcaccatagt tcacatcagt gccaatttct gttgcggatc
    39361 gcgcccttcc atcactaacg gtgcccactc tttgatctgc gcgtgatctt cagagtaacg
    39421 cataccgcga aacagcgagc tttgttgcaa cgcggcgtaa cgggcgcgca ggaaattaac
    39481 gttatcctcg ccccagacaa agctcatatg cggaacggta ttgataaatg aacgcggagt
    39541 acgcagcacg ccgcgctcga cctggtgcgc ccagaactgg cgggaaatct gaaatgcttc
    39601 gttaatggcg actgcttttt caatactgat gctgccatcg gcgttttgcg gggtgtagtt
    39661 cagttccatc agtgcagaat gcccggttcc ggcgttgttc tagccgttcg aactctcctg
    39721 cgcgcccccc tccaggcgct ccaccatggt catcgaccat tcaggctcca gctcgcgtca
    39781 ataggtcccc aacgtggcgc tcataatgcc gccgccaatc aacagtacat cagtttcctg
    39841 ctcctcggac gctttcgctt ttgccgccat cgaaacggca ttaagcccca cggccatcga
    39901 gaagagcatg gcagtcactt ttttcatctt gttaatgcct tacttttagt cgctttattg
    39961 caggtgagat ttgcgcggca tcaacggtaa cacttaagta acaacattta aataatgttt
    40021 ataaattata ttctaatttt agaaacatta taatttcgtt gattaattat agggttatta
    40081 gaaatgaagg gatttttagc atattgacct gacggcagca gtccgccagg tcagcgtcgt
    40141 aatattatgc cgtccgggca acggcatcac gcgaagcggc atcgcgctct tcgcccgtca
    40201 gctcgctaag ttgcccattg cgcatttcca gcaggcggtc ggcgtggata aagtaatgat
    40261 catcatgact gatagcgaaa atagttttac ccatctcctg catcagcggc agcaacacct
    40321 gataaaactc acgacggaag tgtggatcct gatccaccgc ccattcatcc agcaggataa
    40381 tatcgcgttc ttctgccagc gccagcaaca gcgccacgcg ttttttctgc ccttttgata
    40441 acttcaggtt aacaatacgc ccgttgctta actcaagctt atgagccatt ttcagctgcg
    40501 ccagccactt ctcaaccagt tgcgggttag cgggttgccc ctccggcccc agcagttgat
    40561 caaacagcca gacatcggta aacactgccg aaaacagttt gcgataatct tccggttgtt
    40621 cgccgctgac aggtttgcca tccagcaaga tttcgccgct ttgtggctga tacaagcccg
    40681 tcaacaacat cgccagcgtc gattttccgc taccgttacc gccaatcaga aacagcagct
    40741 cgccacgttt gatggtgaga ttaatcggac caacggaaaa cgcgttatcc tgataagcaa
    40801 acgtcacgtt acgcagctcc agcgtttgcc agttgggaaa cgcctgcggg cgcggaaact
    40861 cagctttgaa aggcgcgagc gcgaatttgt tcagcttgtt aaacgccacc tgcgccgtca
    40921 gcagcgtcgg caatgcgcca accgccgaaa gcagcggcgt acgcaggaat aacagcgtca
    40981 acgaataggt cgcagcaacg ttggtatcag cccaaccgag actgttcgcc atccagaaca
    41041 ccaggccgat agcgcccagc atcatgatgt ttgaccagtt cacggcacta agatggaagg
    41101 tgtctgcgcg aataatatga tggcgatact cttgcgcatc ggggatgtag aggttgttaa
    41161 acacatactc ggcgcgttcc cggttcagag tcagctcttt gcgcccttca agtacagttt
    41221 gaaaatccgt gtacagcttg tcttcggttt cacgtagggt cgccatatgt ttgtacaccc
    41281 gcgccaccag cacaaaaccg ccccagatgg tgatcgccat ccagatagcc gttaccagca
    41341 acattttgcc cgacagcatc cacagatacg ccgatgaacc gatagtgaga atgatcccct
    41401 gccccagttc cggcagacgc acaaaagcaa tggtgatatt gcgcacatcg ctggttaatc
    41461 ccgccagcaa cgaggcgcta ccgagttgtt caatgcgctc gacgtgggta tccagaatcc
    41521 gcttgataaa ttcactacgc agtcggtaaa cgaagtgatg ccccaaagtg gtgagcgcca
    41581 gttgcgatcc gagagtgact gccatcaaca gcagcaataa tcccagaaac tccggtaaca
    41641 ccagcagact ggtatccgcc gtttcgataa ggcgctgatt gataaaagca attaagccaa
    41701 tgcctaatgc cgcactggcg aggcttagcg ccatcacact gataaatggc cagcgatact
    41761 gccgccagac aagtacaaga agttccatgc agaaaacccg gacaatgaat tacagcccgc
    41821 agtttaaaca tcttcgcgcg cacagcaata ataattctta tttttattct tttttacctg
    41881 cctgacggaa tgtcaggttg tagcggcagt cggtggtgag tggatgaaac cccgctttca
    41941 acggttgaat accgtgataa aacagccgcg attcaccgcc ccataccacc acatcgccat
    42001 gttccaacaa caaacgtttg agcggatcat tacgtttcag gccaccaaat tgaaaaatcg
    42061 cgggtaagcc cagagaaaca gaaacaattg gcgcgcgcag atccggttcg tctttatcct
    42121 gatgcagcga cagtttcgcg ccaggagcgt agcggttgat aagacaagca tctggctgga
    42181 aatctggata gcccgccgcc gtagccgcac gttgacataa atcatgaaag ctttgtggca
    42241 tagcgggcca cggtttattt gtttgcggat caatgggcga atagagataa ccttgccgat
    42301 gggtcgtcca gcccagatgc ccacagttgg tcatcgccac cgacatggta tatccgcctg
    42361 gagcgaccat ctggcgaaac ggcgactggc tggcaacgtc attaatatcg cggattagtt
    42421 gctccgcagc attaaaagca aaacgccgta aaattaccgc accagccgcc agtggctctt
    42481 gccacggttc agcatcggca aacagatcca acattacctc tcctcatttt cagcttcgcg
    42541 gcgcagcagt tgcgctttac gcgacacgcc ccagcggtaa ccggaaagtg tgccatcacc
    42601 acggaccacc cgatgacagg gtattacgat agccagcttg ttggcggcac aggcgctggc
    42661 aacggcccgt accgctttcg gtttgccgat ggcgttagcc agttgctgat aactgacggt
    42721 ttcaccacaa ggtatcgtgc gcagtgcctg ccagacttgt tgctgaaaag cagtgccgcg
    42781 aatatccagc ggtaacgtca gcggcgtatc gcgttgattg aggctggcga tcacttcacg
    42841 cacatgttgc tgaaacgtca gatcggcagg cgcgttgtcg gcagcgggaa acatctgctg
    42901 caactcgctg attaatgtcg cgtcatcatc gcccagcaat atcgcgcaaa tcccccgctc
    42961 gctttctgcc accaggcaac gacccagctc acaatcagcc agcgcgtaac gcaccgccag
    43021 attttcgcca ccgtgacgga attgtttagc cgtcatgccc agcgtttcat cggctttgcg
    43081 gtagtaactg ctgctgtcgg ggaatccggc gttaagaata gacgtcgtca cgctctcccc
    43141 tttcgccagc gattcgcgca aacggcgagc gcgccaggcc tgttgccagg ctttaggcgt
    43201 cattccggta gtagctttaa acaaccgatg tagatgaaat ggactcatcg ccacctggtc
    43261 ggctaaggct tccagcgtta caggcgtttc ctgttccagc agtcgacacg cgtgggtgat
    43321 tttatccaac cgatgttgcc ggggattggc tttgtctggc tgacaacgtt tgcaggggcg
    43381 aaatccggcg gcgagtgcct cgctggcatt tgcgtagaag gagacatttt cccgcaaggc
    43441 atgtctggcg cggcaagaag gacggcaaaa gatacccgtg gtgcgcacgg cgaaaacgaa
    43501 ttcgccgtcg gcattcgggt cgcgggctaa gacagattgc cagcgttgat cgtcagttaa
    43561 gcatgtggcg tttttcataa tcagctccct ggttaaggat agcctttagt ctgcccggtc
    43621 accatcacgc aaaaaccaac aatcttgcgc tttaattttt ttcgctgaca aggaagcttt
    43681 taaactgtgg tgacatccag gttttaaagc tatcgccttc tttggtgatc atataaaccg
    43741 ccagcccttc ccggcgaaca acctctttgg ctttctccgg cccgagtacc atcaagccag
    43801 tatcccaggc atcggcttcc agcgccgtcg gagcaatcac cgtcacggat accagattgt
    43861 gttcgatggg acgcccggtt tgcggatcga taacatggga aagacgtttg ccgtccagtt
    43921 cgtaatagtt acggtagctg ccagaggtgc taatcccatg accgttgata tccaccacag
    43981 cctgaaccgc gttttcttta tcggttggtt tttgaatcgc tacccgccac ggctggcctt
    44041 caccgttcat accacggctg ttcagcgcgc cgcccaccga caccagatag cgggaaatcc
    44101 cttcctgctc catcaagcgt gccaggtgat ccgccgcata accttcaccg acggtggaga
    44161 gatcgacata taaatccggc aggtcttttt gcagatattg ctgatgcgac tgattgatga
    44221 ccgtcaggtg ctgtaagccg gttttggctt tcatcgcatc gatctgttcc tggctcggaa
    44281 tttgaaccgg ctgttgttcc gggccaaagc cccacagatt caccagcggc ccgacggtaa
    44341 tatccatcgc accatcggtc cttgccccaa tgcgcagcga ggtggtgacg atatcggcca
    44401 tcgcttcact taccggccac ggcgacaaac tttgcgagtc gttaaagcgc atcagcgcgg
    44461 aatctttttt ataggtcgaa agcagctgat cgtcggcgtc cagctgggtc tgaatctttt
    44521 ctttaagttc ggcactgcgt ttggcatcaa tgcccgggat gctggcacgc cagaaggtac
    44581 ccatggtttt gccttcaaga acggtaactt cagtagcgtg ggttttggcg ggttgtggtt
    44641 tttgatcgca accaacaaag aagagcgcgg caaccagcag tgccacgcgg gtaaagctta
    44701 tttccatacg tgattatcct catgcgaacg gtcgcaagag tacaccaaaa aactgtgttt
    44761 gtacgctgaa aacaatgaaa aaagggcccg caggcccttt gttcgatatc catcgagatt
    44821 agaactggta aaccagaccc agagctacga tgttatcagt gttgatgcca gcgtcacgag
    44881 tgaactggtt gtcgtccagc aggttgattt tgtagtcaac gtaggtggac atgtttttgt
    44941 tgaagtagta ggtcgcacca acatcaacat atttcaggat atcttcgtcg tcgtagttac
    45001 gaccattgat gacacccagg tttttacctt tagactgcag gtaagccagg gacggacgca
    45061 gaccgaagtc gaactggtac tgagcaacag cttcgaagtt ctgtgctttg ttcgcccaac
    45121 ccaagttgcc tacgcgagtt gcgttgtagg tctgggtgta ctgagcagct aggtagatgt
    45181 tgttcgcgtc gtatttcaga ccaccggtgt aggtttcagc gcggtcgcca ttaccgatgt
    45241 aacgctcatc ataacggttc agaccgaagt tctggtcatc agtacgtttg gagctggaaa
    45301 ctgcagcacc gataccgaag ccttcgtaat cataagtaat agaaccgcca acgccgtcac
    45361 cgttctgacg cagagcttca cgaccgttgt tggtcatgcc ttcaccttcc gggctaccgt
    45421 ttttgccctg atactgaaca gcgaagttca gaccgtcaac cagaccaaag aagtcggtgc
    45481 tacggtaggt tgcgaagccg ttaccacgct gctgcatgaa gttgtcagaa ccgtaagtgt
    45541 caccaccgaa ttctggcagt acgtcggtcc aggaagttac gtcgtaaact acgccgtagt
    45601 tacgaccgta gtcgaaagaa cctacatcct ggaatttcag acctgcgaat gccacacggg
    45661 tccaggagtt gttttcgttt tcagcgctgt tgccctggat ctgatattcc cactggccgt
    45721 aaccggtcag ctggtcagta acctgagttt cacctttgaa gccaagacgc atgtaggtct
    45781 ggtcgccatc tacagacttg tcgtcagaga aatagtgcag gccgtctact ttaccgtaca
    45841 gatctaattt gttgccgtct ttgttgtaaa cttcagcagc gtttgctgcg cctgctacca
    45901 gcagagctgg gaccaggagg gacagtactt taactttcat gttattaacc ctctgttata
    45961 tgcctttatt tgctttttta tgccactgca tactgattaa ccctcattaa tcagtcggca
    46021 agtccattct ccccaaaaat gcagaataat ccaacacgaa tatgatacta aaacttttaa
    46081 gatgtttcat ttatcgctat agatgtttca aaatgtaaat acaagggaac tttttaagat
    46141 tattgcggaa tggcgaaata agcacctaac atcaagcaat aataattcaa ggttaaaatc
    46201 aataacttat tcttaagtat ttgacagcac tgaatgtcaa aacaaaacct tcactcgcaa
    46261 ctagaataac tcccgctatc atcattaact ttatttatta ccgtcattca tttctgaatg
    46321 tctgtttacc cctatttcaa ccggatgcct cgcattcggt ttttttaccc ttctttacac
    46381 acttttcatt attctgtgct accacagaaa aactataacg cttgttaact atttcacaaa
    46441 taattaacat cagcataatt tccagcaatc tttgtttatt tgcaattaat ttcgttgggc
    46501 tttttgtagg ttatttgtac agcaaaatgg cgcttgtaca tctatttccc cccatacagg
    46561 atgataaata tcacgggaga atagagaatc atcaatcagg taagagtctg gaatttcaca
    46621 ctgtaccctt tatactgccc tatcacttcg cgaagtttta acaggtcata aacacgaatg
    46681 cgtcagaaag agacaacggc cacgacccgc ttttcactcc taccggggag cattacccgc
    46741 ttctttttac tgttgatcat tgtgttactg gtgacgatgg gggtaatggt acaaagtgcc
    46801 gttaacgctt ggctgaaaga taaaagttac cagattgtcg acattaccca cgcaatccaa
    46861 aagcgcgtcg atacctggcg ttacgtgacc aggcagatct acgacaacat tgccgcgacg
    46921 acctccccct cctccggcga aggtttacaa gagacgcgcc tgaaacagga tgtctactat
    46981 ctggagaaac cacgccgcaa aacggaagcg ttaatctttg gctctcacga caactcaacg
    47041 cttgagatga ctcagcggat gtccacttat ctggatacat tgtggggtgc agaaaatgta
    47101 ccgtggtcga tgtattacct gaatggtcag gataacagtc tggtgctgat ctcaacccta
    47161 ccccttaaag atctcacctc cggatttaaa gaatcgaccg tcagtgacat tgttgattca
    47221 cgtcgtgcag aaatgttaca acaggccaac gccctcgatg aacgcgaaag tttttctaac
    47281 atgcgccgcc tagcctggca gaacggtcat tactttacct tgcgtaccac gttcaaccag
    47341 ccgggacatc tggcaacggt cgtggctttt gatctgccga ttaatgattt gatcccaccg
    47401 ggtatgccgc tggacagttt ccgccttgag ccagacgcga cggcaacggg aaacaatgat
    47461 aatgagaaag aagggacgga tagcgtcagt atccacttta acagtacgaa gattgaaatc
    47521 tcctcggcac tcaactctac cgatatgcgc ctggtctggc aggttcctta tggcacctta
    47581 ttgctggata cgttgcaaaa cattctgctg ccactgctgc tgaacatcgg tttgctggcg
    47641 ctggcgttat ttggctatac cacattccgc catttctcca gccgcagtac agaaagcgtc
    47701 cccagcacgg cggtcaataa cgaattgcgc attttacggg caatcaatga agagatagtc
    47761 tcactgttgc cgctcggcct gctggttcac gatcaggaat cgaaccgcac tgtcataagt
    47821 aacaaaattg ccgatcattt gctgccgcat ctgaatctgc aaaacatcac caccatggcg
    47881 gaacagcatc aggggattat tcaggcgacg atcaataacg agctgtatga gatccgcatg
    47941 ttccgcagcc aggtcgcgcc gcgcacacaa attttcatta ttcgcgatca ggatcgcgaa
    48001 gtgctggtaa acaagaaact caagcaggcg cagcgtctgt atgagaaaaa ccagcagggg
    48061 cggatgacct ttatgaaaaa cattggcgat gcgctgaaag aacccgcaca gtccctggcg
    48121 gagagtgcgg ctaaactcaa cgccccggaa agcaaacaac tggcgaatca ggcagatgtg
    48181 ctggtgcggc tggtcgaaga aatacagtta gcgaacatgc ttgcggatga tagctggaaa
    48241 agtgagacgg tgctgttctc cgtgcaggat ttaattgatg aagttgtgcc ttcagtgttg
    48301 cctgccatca agcgtaaagg tctgcaactg ctgattaaca atcatctgaa agcacacgat
    48361 atgcgccgcg gcgatcgcga tgccttacga cgtattttgc tgctactgat gcaatatgcc
    48421 gtgacctcaa cgcaattggg aaaaatcacc cttgaggttg atcaggatga gtcctctgaa
    48481 gaccgcctga cattccgcat tctggacacg ggagaaggcg taagtattca tgaaatggat
    48541 aatttgcact tcccgtttat caaccagacc caaaacgatc gttatggaaa ggcggacccg
    48601 ctggcattct ggctgagcga tcaactggca cgtaaactgg gcggtcattt aaacatcaaa
    48661 acgcgggatg ggcttggtac acgctactct gtgcatatca aaatgctcgc cgctgacccg
    48721 gaagttgaag aggaagaaga gcgtttactg gatgatgtct gcgtaatggt ggatgttact
    48781 tcggcagaaa ttcggaatat tgtcactcgc ctgttagaaa attggggtgc aacctgtatc
    48841 acacccgatg aaagattaat tagtcaagat tatgatatct ttttaacgga taatccgtct
    48901 aatcttactg cctctggctt gcttttaagc gatgatgagt ctggcgtacg agaaattggg
    48961 cctggtcaat tgtgcgtcaa cttcaatatg agcaacgcta tgcaggaagc ggtcttacaa
    49021 ttaattgaag tgcaactggc acaggaagag gtgacagaat cgcctctggg cggagatgaa
    49081 aatgcgcaac tccatgccag cggctattat gcgctctttg tagacacagt accggatgat
    49141 gttaagaggc tgtatactga agcagcaacc agtgactttg ctgcgttagc ccaaacggct
    49201 catcgtctta aaggcgtatt tgccatgcta aatctggtac ccggcaagca gttatgtgaa
    49261 acgctggaac atctgattcg tgagaaggat gttccaggaa tagaaaaata catcagcgac
    49321 attgacagtt atgtcaagag cttgctgtag caaggtagcc tattacatga acaatatgaa
    49381 cgtaattatt gccgatgacc atccgatagt cttgttcggt attcgcaaat cacttgagca
    49441 aattgagtgg gtgaatgttg tcggcgaatt tgaagactct acagcactga tcaacaacct
    49501 gccgaaactg gatgcgcatg tgttgattac cgatctctcc atgcctggcg ataagtacgg
    49561 cgatggcatt accttaatca agtacatcaa gcgccatttc ccaagcctgt cgatcattgt
    49621 tctgactatg aacaacaacc cggcgattct tagtgcggta ttggatctgg atatcgaagg
    49681 gatcgtgctg aaacaaggtg caccgaccga tctgccgaaa gctctcgccg cgctgcagaa
    49741 agggaagaaa tttaccccgg aaagcgtttc tcgcctgttg gaaaaaatca gtgctggtgg
    49801 ttacggtgac aagcgtctct cgccaaaaga gagtgaagtt ctgcgcctgt ttgcggaagg
    49861 cttcctagtg accgagatcg ctaaaaagct gaaccgcagt attaaaacca tcagtagcca
    49921 gaagaaatct gcgatgatga agctgggcgt cgagaacgat atcgccctgc tgaattatct
    49981 ctcttctgtg accttaagtc cggcagataa agactaatca tctgtaggcc agataagacg
    50041 cgttagtgtc ttatctggca tttgcaccga ttgccggatg cggcgtaaac gccttatccg
    50101 gcctacgatt cccattattt caacagatta cattaaagta ggccagataa gacgcgtcag
    50161 tgtcttatct ggcatttgca ctgaatgccg gatgcggcgt aaacgcctta tccgtcctac
    50221 gaatcccgcg atttcctgac cctctcggca tataccgtca gcgtctgttt tatcacatcc
    50281 agcgttaccg gcttcgagag gcagctgtcc ataccggact ccagacaccg ctgcttctct
    50341 tcagccaacg cattagcagt tactccgatt accggcaacg tcagtcccaa ctgacgaatg
    50401 cgttgcgtca agcggtaacc atccatattt ggcatgttga cgtcgctaag cacgatatca
    50461 atatgattct tactaagtac attaagcgca tcgacgccat cattcgcggt tttacattga
    50521 tagcccaacg atcccaactg atctgccagc agacgtcggt taatcggatg atcatccacg
    50581 accagaatca tcatatcgtc gttatcgctg accgctttgt ccgtcgacgg cagagcgtta
    50641 gcaggatcgt cgctctccat ctcgatcaaa taaatacgcg ccaacaatgc cggtagctca
    50701 tgcggagcag ccacactgtg tacccactcc cctggcgctt tctccagcgg aataccaata
    50761 tggcgacgac agaaggtcac taccgctctg ccctgccatt ttttactcac tacctcgtca
    50821 gtgattaaca catcttcggg agtcggttcc tgcccttcgt atgttgtaac gacgatgccg
    50881 ctgcgctgca aactggtttc caggaactga cagagcgacg cattgcggac cgccagccag
    50941 cagcgtttac cactcaaccc ttccacgcct tttttctgcg ggtactgagc gccgtacaac
    51001 ggaatacgca cggtaaactg gctgcccatt cccggttctg aatctaccga gatatcgccg
    51061 tccatcatgc tgatcagttt ttcacaaatc gccagaccca gaccggtccc ctggaagttt
    51121 cgctgtacgc ccgttccaac ctggaagaag ggatcaaaca agcgcaccac ttctttcgcc
    51181 ggaatcccca cgccggtatc gcgaacacgg atagagagat aatcgccatc agcgcgaaca
    51241 tgcaaaacta tacagccggt atcggtgaat tttatggcgt tactcaacag gttggagatg
    51301 acctgctgta aacgcatcgg gtcgccattt aaggccactg gcacatccgg ttcaataaag
    51361 cagtacaagc ctaactgctt gcgtaccacc agcggtaaat agttggcggt gatgtggttc
    51421 atcacttcac gcggtgaaaa ctcacgcggt tcgatcttca actgttccga ttcaatcttc
    51481 gagaaatcga gaatatcgct gataattttc aacaacaggc tggaagagtt gttcattgcc
    51541 gtcaccagac gatcgacgcc tttcggtaac tctttggttt gcaacagatc caggttaccg
    51601 ataatgccat acagcggcgt tcgcagctca tgactgacgg tggcaaggaa catcgatttt
    51661 gactggctcg cctgttccgc tgcttgtgcc atctcctgca acgactcttc catcttcacg
    51721 cgcgaagaaa catccaccag cacacaaatg gccacgtttt cattacgata gcgcgaatgg
    51781 acgaagctga tttgcagatt ggtattgttg ctggtcagga catcaacaaa attgacctgc
    51841 tgcccacaga taatttgcgt cagtcgttgg cggtcctcat gcgtaagcat attgagatag
    51901 gtatgcgcca gttcgttact taaaatattg acgccatcag cggtacgcaa aatgcagata
    51961 cccactggcg cggaggcgac aatcttgcga ttgaactgct catgttcttc cagtcgcagg
    52021 gcgtcgcttt ccgccggaat gaaaatacgt cgctcgtaca tccgcgcgag agtaaacaat
    52081 gcagctccgg caagcacatt cagcaaaatt gcgttaagga tcaacatgcg aatgcgttcc
    52141 agcaccttat caaccggcac cgaatacacg atgcttagcg atgagggtgg cagatttttc
    52201 ttcagcacca gctcccggaa cccttccgta tagccaaacc aggagcgttc ctgcatccag
    52261 cgaggatcgc ccttaatttt actttctggt ccggtaagcg aaatcagggt atgaccattt
    52321 tcatcaagaa tggtaacccc catcggcaac gtacccggta agaaaaagtt ctccatccgg
    52381 atggtctgct cgacacccaa aagcgcctgc aaccggttcg ccagataaac tggcgtcaac
    52441 gcgtaaaaat acccgacgca agggcgcgga ccttcgctga tccagtagag gttactgccg
    52501 ctatcatctt gtggtgcatt tcgatattta ttgatgcgtt catgcaacgc tttcaacgcg
    52561 gtatcgcgtt ccactggcat atcacgcaga ccgaaattgg ccatgcagag gttatcactg
    52621 ccgattaaaa atacccggtt gagatcgtaa gccgcagaaa aattatcgcg ccagtagcgc
    52681 ataaaccacg ccaatgactc cagagaacct cgccaggtgt tactcattgc ggaacaatcg
    52741 gagtcggcaa acagcggttc aaacgcaggc acatccgcct gcgtttctcg tccacgcggg
    52801 gaaagcacac cgttttctgc cgataagcga ttttcggcga tgtacttaag ctctttcatc
    52861 acatcagagg tgcgttgaat aaagcgctga gcctgatcgg aactcagatt aaattcctga
    52921 cgaatttccg attctcgctg atgtaacgca ttaacgatgt aaaaaacgga tgaaaaagca
    52981 atcaacagcc agagcactaa cgccaatgct ctgaacatgt agcgcgaggc tttcagggtt
    53041 gtacgaaaag aagcaaggta tttcaaaggg gagaagctcc gcctcaggtg accgatggag
    53101 tgtggttaag gtagcggtaa aagcgtgtta ccgcaatgtt ctgtcaccat tgcaataatg
    53161 cgtatcgctt agaaaccatt gccttaacca ggaaagccgg agtagatgga cagttctcag
    53221 tacgaaaaat aagaaatgac caatctgccg acgcttgtta atggttatat cctggcgtcg
    53281 gcagatcgcc cagcagtctt atctgaaaaa agagagagcc aggttgtgtg aagggatgga
    53341 tcaccagtca aaacttttca ccagcgtcag ctcgccagca ttacgcatcg gtacaataaa
    53401 tgtttcctgt ttctcattga ccgatccttc atcggtgatc agcgtcagtt gggcggtcgt
    53461 caattccgtt tcgctgcgcc caccatagta gttgatatac acctgatagc gcccgtgaac
    53521 tggtgctggc atggcgaaaa tctcgggtcc gtaccccgtc gtgacatcca tatccagtgc
    53581 accactgttt ttcagcacgg tgttaccgta ccaggcgtgt tcgccatcgg gcgtgacaac
    53641 atggagatcg agatcggtat tgtccgtatc ccacgagaga accagccgta aacgtgcacg
    53701 aatagttcct gtacccggcg ttgagtaaaa ctgcattttt tgccggcttt gcccatccgg
    53761 gctgatgacc tgcacgctgt tgctgccttc agtgaaaata taagggcgtg caaatgaacc
    53821 atcggattca attctctgcg gcatactggc accgttgacc accagccggc cctgctgaac
    53881 tttacccgcc gcagcataat tctttatttt gccgcggatc tgcgctgaga tattttgatc
    53941 atctgccatg ttgacggacg acgccgggta attgatcgat tgtgaaaaac tggcatcttc
    54001 gccctctgcc ggatgccagc cagagagcgg tgcgtcaatc tcgacttcct gcacaccttc
    54061 actgtgagcg acaggcgaaa gtgccaccag taacaacgga agaaaaatct ttcgcatagc
    54121 attagtccat taataattgt cgggtcagcg tttcgatata gttctcatca agcccggtgg
    54181 gataaccatc aaaggccaag tgcagatact catgggttaa atccagccga tcctgaagcg
    54241 taaagaagtt acgaataaac agccgtttct gctgccgatc ggtatagggg aaacctgaga
    54301 ctaaacgaca gaccgcaaac acgtctggtt cgttgtaccc cgtctcgcct tgtaatatac
    54361 gccgccactg cggcattttt ttcgccagcc aggctttcgc tttgggtaat aactggcagg
    54421 ttgagcgtgg agctccccag cggctaaggc tgttgtcggg ataagcaaac gcgagtatct
    54481 ggtcgtaacg ctccccctgc cccgcctggg ccgtggcttg ccgccaggaa agtgtccctt
    54541 cggtagcgcg actgccatga taatgaaccg gatcgcctgc atagatgagc tcctgcgtcc
    54601 aggcggtcat cgttcgcgcc cccgtggtcg ctggcgaagc agaaacacgc tgcgtggcgc
    54661 tgctgtcggg gatggtcagg caatcacctt cccgattggc gttttgctgt aaaaaagtac
    54721 gtatcgccac agtcatggct ttcgctgcct caggtggcgt acttttcgcc tcgcgatcca
    54781 gaactctggc aacgtactct tcacgatcga gatgcgactg caatttcagt ttgccctttt
    54841 cgctcagtaa ggtcgtctca ccgtggctga caaacgtaat gtggttacca ttagtaaacg
    54901 tcacacgata gcgcccatta agtacgctgg gttttactgc cgtcgtactc ttttccgctg
    54961 tgattttctt cagcggatag cgggcaaaca gctccacctc tacacattgc ccactggcaa
    55021 cctgcgtcgg cactggcaaa acccgattaa gcaccgtcgc ataacgggtt agtaccgttt
    55081 tgctggtgcc actgccagta acccacagcg gtgtaccatc ggttaaccag ccagcaaaac
    55141 caccctgacg gatttcctgt ttatcatcgg caaaccagct ccaggttttc acccgtaccc
    55201 gactaccgag catggatgcg acaccgattt tcgcctcgtc cagcaccaca tccagtaaga
    55261 cttcctgagc tttgttttgc gcaggtaacg tcgctaatga atcaagcagc gatttaaccg
    55321 ttaccgaggt ttctggcttc agcgttgtaa gagacgctaa ccacgcaggt gcctgacgct
    55381 gttgccagta ttgcccccac acatctgcgc caatatgcaa ccgctgcggc gcaaaataga
    55441 gcccacagga acgtaccagc gcggtatcgc gcgttatgct ctccccagcc tgacaacaat
    55501 aaacctcttc cggggagttt ccgcgacact ggtagggctg ctccggttga tgggtatcct
    55561 ccagccaggc gtaaacatac agtttccaca aactccctaa cggcgtggtg agcgagtcag
    55621 gtaatgctga gactttggtc acgcctgatg acgacagctg atacagttga tcgtgctgcg
    55681 caccgcgcag caccagttgt aacggcgtct cctccgcaag cgttggcaga gtaaccaacg
    55741 ccaatagcca gacgattctg cgccagttca cttatttcac ctgcatcccg gtccattcac
    55801 tccctgccgc gacactttgc tgcgcaggtg cataggaacg cacataacga gcaggaggca
    55861 gaacgaattg ccctttttgc gagaagcgca gcaaatggcg gaaagtgacc gttcccgtca
    55921 gttctttcac cggcaccata taggccagtt cgcccatttc attacgcgct ttttccagca
    55981 attgcccctg ctgtttcgcg gcgttgggtt tattgactga aatgccccat gttgtgcgct
    56041 caacgtcagc tcccggcggg agcggtactt ccacctgacc atagcgcaga actgcatcct
    56101 gctcgctggt aagcgtaatt tcatcgagat acagcgcatc gctgtcaatc tcattgctgg
    56161 tcaccggctg cagagtaaag ctcatctctt cttcaccggg gataagccga tacaactggc
    56221 gttcaacggt caccggaatg ttactttgtt gagccgtttt tgccggttca cgccagcgga
    56281 cctgcacatt tgcggcgata attcgtcacc aaaagagaga atgtccggca caccctaacc
    56341 aacccaacgc cagtcttcgc cccctccagt taacttatgt ttagcccatg cgcccgcagg
    56401 cgcaggcaaa acaactggag gcattgtcgc catatatttc gccagccagt tcatggccag
    56461 cgcgcgctca atagtggatt gctcagcggt caaaccatta aaatagcggc cgtctgggta
    56521 gcgtcaccac cgctattgag cagcacaacc gtgtgcgcca gcggctgatt gctgtttgcc
    56581 gctttatccc acgcatattg ctgaacctgt ggcatcaccg tggacttcaa ctgcgctttt
    56641 ttcagcaacg tcatggttaa gacgtttgcc accgcatctg ccagtggaga ttccggtgta
    56701 tcgaggatca ggctgtcatt gatatcgcgg atgtcttcct cagagaaatc ttcatcttta
    56761 gttccgcgcc gggcgatagc ttcatccccc cctttcaaca acgttttgca cggcagattc
    56821 atctcctgtg cccacgccag caccagcgcc cgatgcaata acggcatatt atccgcctgc
    56881 tcggcataac tgtcgagcat atgctgccag tattccggtt gttgcgttac gccgagcgcc
    56941 tggctggcct gccagtcggc gtaccatgcc catgccgtaa ggaaggcgtc accattgcca
    57001 tcttcacccc accaggtaaa gcgcgctccg ggccccgcca gttgcatcag ccgcagacgg
    57061 ttatcctgaa tcatctgacg aatgtcgtta gcggcggcac tttgatgatc ggcaagcgaa
    57121 cgccaggcga gactgagcgg gatcagacgg ctaccggtgt tgatcacccc accccacggt
    57181 tcatcgacta acgcatcaag attgttgcgg aaaatctcct gcggcgtttc actactttgt
    57241 agccggatat tactcgcctg ctcgggcaac atcagcgcgt tctcgccacc gccgagcatg
    57301 acatttttct gctgttcaac gggccagctg ttgtccacaa aagacagttt tgtgctaatg
    57361 ctgtcctgca cttgcccatt ttgttgcagt tctgcactta acaagccaga ttgctgaata
    57421 ttctgcgtca gcgaaatata attcgcccct ttgtgcagcg tcagcgtctg gcgcatctca
    57481 gcgcctgcaa atttagtcac cagcgccacc ggttcgttat cctgctgact gaagataaac
    57541 agtcctgccg ccggtttgtc gcccacgcga tacaccgttg gcatactcca cttcatgtag
    57601 agattttttt ccgaacgcag ataagcacgc ccctgcccga ccagcccgtc gccgttcatc
    57661 ccacgcgcgg tgatacgcca gcgggttaac gaatcaggca tcaggaacgt gaaatacgct
    57721 ttgccttgtt tatcggttgt gagtgacggc atccatgccg cggtatccac ctcttcacgc
    57781 cgtggacgtt caagcatttt tactcgccgc tcgctgcggt tagtcgcgcc aggcgcaacc
    57841 ggctcgctgg agagcgcctg gtcatagctg ataaacgaca gactggagct ggtacgcaca
    57901 ttgttacgcc ccagcggata gaaaaatttg ccgatattcg gcgcgatttc tggttgcagc
    57961 gcgtagatca tttcatcgac cacgcctacc gttagctgcg cagaaacagg tttacctttc
    58021 agcgacgagg tta
//
LOCUS       DAJTPG010000013        55236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTPG010000013 DAJTPG010000000
VERSION     DAJTPG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO230_000855"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO230_000855"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5690720.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO230_000856"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO230_000856"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5690721.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO230_000857"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO230_000857"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5690722.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO230_000858"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO230_000858"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5690723.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO230_000859"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO230_000859"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5690724.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO230_000860"
     CDS             7826..9559
                     /locus_tag="OO230_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5690725.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO230_000861"
     CDS             9708..10559
                     /locus_tag="OO230_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5690726.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO230_000862"
     CDS             complement(10546..10887)
                     /locus_tag="OO230_000862"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5690727.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO230_000863"
     CDS             complement(10889..11767)
                     /locus_tag="OO230_000863"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5690728.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO230_000864"
     CDS             complement(11733..14030)
                     /locus_tag="OO230_000864"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5690729.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO230_000865"
     CDS             complement(14081..14401)
                     /locus_tag="OO230_000865"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5690730.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO230_000866"
     CDS             complement(14416..15495)
                     /locus_tag="OO230_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5690731.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO230_000867"
     CDS             15804..16907
                     /locus_tag="OO230_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5690732.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO230_000868"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO230_000868"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5690733.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO230_000869"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO230_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO230_000870"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO230_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO230_000871"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO230_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO230_000872"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO230_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5690734.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO230_000873"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO230_000873"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5690735.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO230_000874"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO230_000874"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5690736.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO230_000875"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO230_000875"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5690737.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO230_000876"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO230_000876"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5690738.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO230_000877"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO230_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5690739.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO230_000878"
     CDS             30120..30728
                     /locus_tag="OO230_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5690740.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO230_000879"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO230_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5690741.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO230_000880"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO230_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5690742.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO230_000881"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO230_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5690743.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO230_000882"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO230_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5690744.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO230_000883"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO230_000883"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5690745.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO230_000884"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO230_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5690746.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO230_000885"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO230_000885"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5690747.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO230_000886"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO230_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5690748.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO230_000887"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO230_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5690749.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO230_000888"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO230_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO230_000889"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO230_000889"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5690750.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO230_000890"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO230_000890"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5690751.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO230_000891"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO230_000891"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5690752.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO230_000892"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO230_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5690753.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO230_000893"
     CDS             complement(44843..45142)
                     /locus_tag="OO230_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5690754.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO230_000894"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO230_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO230_000895"
     CDS             45895..46494
                     /locus_tag="OO230_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5690755.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO230_000896"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO230_000896"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5690756.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO230_000897"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO230_000897"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5690757.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO230_000898"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO230_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5690758.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO230_000899"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO230_000899"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5690759.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO230_000900"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO230_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5690760.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO230_000901"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO230_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5690761.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO230_000902"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO230_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5690762.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO230_000903"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO230_000903"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5690763.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..>55236)
                     /gene="yiiM"
                     /locus_tag="OO230_000904"
     CDS             complement(54612..>55236)
                     /gene="yiiM"
                     /locus_tag="OO230_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5690764.1"
                     /translation="GKPSAIAKIQVDGELMLTELGLEGDEQAEKKVHGGPDRALCHYP
                     REHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGDIFRWGEALIQVSQPRSP
                     CYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPLELVSRVSDVTVQEAAAI
                     AWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFSRRLWGK"
BASE COUNT        13448 a        14971 c        14125 g        12692 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaatacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgcct
//
LOCUS       DAJTPG010000014        53130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTPG010000014 DAJTPG010000000
VERSION     DAJTPG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..192)
                     /locus_tag="OO230_000905"
     CDS             complement(<1..192)
                     /locus_tag="OO230_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5690765.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAG"
     gene            complement(205..1986)
                     /gene="gcl"
                     /locus_tag="OO230_000906"
     CDS             complement(205..1986)
                     /gene="gcl"
                     /locus_tag="OO230_000906"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5690766.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2076..2891)
                     /gene="allR"
                     /locus_tag="OO230_000907"
     CDS             complement(2076..2891)
                     /gene="allR"
                     /locus_tag="OO230_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5690767.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2969..3451)
                     /gene="allA"
                     /locus_tag="OO230_000908"
     CDS             complement(2969..3451)
                     /gene="allA"
                     /locus_tag="OO230_000908"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5690768.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3681..4607
                     /gene="allS"
                     /locus_tag="OO230_000909"
     CDS             3681..4607
                     /gene="allS"
                     /locus_tag="OO230_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5690769.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            4676..5770
                     /gene="mnmH"
                     /locus_tag="OO230_000910"
     CDS             4676..5770
                     /gene="mnmH"
                     /locus_tag="OO230_000910"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5690770.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(6483..8898)
                     /locus_tag="OO230_000911"
                     /pseudo
     CDS             complement(6483..8898)
                     /locus_tag="OO230_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(8895..9581)
                     /locus_tag="OO230_000912"
     CDS             complement(8895..9581)
                     /locus_tag="OO230_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5690771.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            9552..10174
                     /gene="tesA"
                     /locus_tag="OO230_000913"
                     /pseudo
     CDS             9552..10174
                     /gene="tesA"
                     /locus_tag="OO230_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            10164..10973
                     /gene="ybbO"
                     /locus_tag="OO230_000914"
     CDS             10164..10973
                     /gene="ybbO"
                     /locus_tag="OO230_000914"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5690772.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            11034..11888
                     /gene="cnoX"
                     /locus_tag="OO230_000915"
     CDS             11034..11888
                     /gene="cnoX"
                     /locus_tag="OO230_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5690773.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(11951..12730)
                     /gene="fetB"
                     /locus_tag="OO230_000916"
     CDS             complement(11951..12730)
                     /gene="fetB"
                     /locus_tag="OO230_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5690774.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(12717..13394)
                     /gene="fetA"
                     /locus_tag="OO230_000917"
     CDS             complement(12717..13394)
                     /gene="fetA"
                     /locus_tag="OO230_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5690775.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            13540..14457
                     /locus_tag="OO230_000918"
     CDS             13540..14457
                     /locus_tag="OO230_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5690776.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            14454..14912
                     /locus_tag="OO230_000919"
     CDS             14454..14912
                     /locus_tag="OO230_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5690777.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(14913..15320)
                     /gene="cueR"
                     /locus_tag="OO230_000920"
     CDS             complement(14913..15320)
                     /gene="cueR"
                     /locus_tag="OO230_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5690778.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(15445..16737)
                     /locus_tag="OO230_000921"
     CDS             complement(15445..16737)
                     /locus_tag="OO230_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5690779.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(16740..17672)
                     /gene="glsA"
                     /locus_tag="OO230_000922"
     CDS             complement(16740..17672)
                     /gene="glsA"
                     /locus_tag="OO230_000922"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5690780.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            17934..20438
                     /gene="copA"
                     /locus_tag="OO230_000923"
     CDS             17934..20438
                     /gene="copA"
                     /locus_tag="OO230_000923"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5690781.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(20653..20994)
                     /locus_tag="OO230_000924"
     CDS             complement(20653..20994)
                     /locus_tag="OO230_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5690782.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            21132..21926
                     /locus_tag="OO230_000925"
     CDS             21132..21926
                     /locus_tag="OO230_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5690783.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            22130..22609
                     /gene="ybaK"
                     /locus_tag="OO230_000926"
     CDS             22130..22609
                     /gene="ybaK"
                     /locus_tag="OO230_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5690784.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(22646..24298)
                     /gene="ushA"
                     /locus_tag="OO230_000927"
     CDS             complement(22646..24298)
                     /gene="ushA"
                     /locus_tag="OO230_000927"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5690785.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            24516..25736
                     /gene="fsr"
                     /locus_tag="OO230_000928"
     CDS             24516..25736
                     /gene="fsr"
                     /locus_tag="OO230_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5690786.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            25974..27650
                     /locus_tag="OO230_000929"
     CDS             25974..27650
                     /locus_tag="OO230_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5690787.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27783..29087)
                     /gene="gsk"
                     /locus_tag="OO230_000930"
     CDS             complement(27783..29087)
                     /gene="gsk"
                     /locus_tag="OO230_000930"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5690788.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            29239..30198
                     /gene="aes"
                     /locus_tag="OO230_000931"
                     /pseudo
     CDS             29239..30198
                     /gene="aes"
                     /locus_tag="OO230_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            complement(30195..31157)
                     /gene="hemH"
                     /locus_tag="OO230_000932"
     CDS             complement(30195..31157)
                     /gene="hemH"
                     /locus_tag="OO230_000932"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5690789.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(31289..31933)
                     /gene="adk"
                     /locus_tag="OO230_000933"
     CDS             complement(31289..31933)
                     /gene="adk"
                     /locus_tag="OO230_000933"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5690790.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            complement(32114..33988)
                     /gene="htpG"
                     /locus_tag="OO230_000934"
     CDS             complement(32114..33988)
                     /gene="htpG"
                     /locus_tag="OO230_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5690791.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(34098..34703)
                     /gene="recR"
                     /locus_tag="OO230_000935"
     CDS             complement(34098..34703)
                     /gene="recR"
                     /locus_tag="OO230_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5690792.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(34703..35032)
                     /locus_tag="OO230_000936"
     CDS             complement(34703..35032)
                     /locus_tag="OO230_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5690793.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(35085..37010)
                     /gene="dnaX"
                     /locus_tag="OO230_000937"
     CDS             complement(35085..37010)
                     /gene="dnaX"
                     /locus_tag="OO230_000937"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5690794.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    complement(35688..35752)
                     /gene="dnaX"
                     /locus_tag="OO230_000937"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(37139..37690)
                     /gene="apt"
                     /locus_tag="OO230_000938"
     CDS             complement(37139..37690)
                     /gene="apt"
                     /locus_tag="OO230_000938"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5690795.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(37843..38220)
                     /locus_tag="OO230_000939"
     CDS             complement(37843..38220)
                     /locus_tag="OO230_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5690796.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            38290..38817
                     /gene="priC"
                     /locus_tag="OO230_000940"
     CDS             38290..38817
                     /gene="priC"
                     /locus_tag="OO230_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5690797.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38831..38992
                     /gene="rsmS"
                     /locus_tag="OO230_000941"
     CDS             38831..38992
                     /gene="rsmS"
                     /locus_tag="OO230_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5690798.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(39204..42566)
                     /gene="mscK"
                     /locus_tag="OO230_000942"
     CDS             complement(39204..42566)
                     /gene="mscK"
                     /locus_tag="OO230_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5690799.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(42694..43341)
                     /gene="acrR"
                     /locus_tag="OO230_000943"
     CDS             complement(42694..43341)
                     /gene="acrR"
                     /locus_tag="OO230_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5690800.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            43483..44676
                     /gene="acrA"
                     /locus_tag="OO230_000944"
     CDS             43483..44676
                     /gene="acrA"
                     /locus_tag="OO230_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5690801.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            44699..47848
                     /locus_tag="OO230_000945"
     CDS             44699..47848
                     /locus_tag="OO230_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5690802.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            48394..48768
                     /gene="tomB"
                     /locus_tag="OO230_000946"
     CDS             48394..48768
                     /gene="tomB"
                     /locus_tag="OO230_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5690803.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            48794..49012
                     /gene="hha"
                     /locus_tag="OO230_000947"
     CDS             48794..49012
                     /gene="hha"
                     /locus_tag="OO230_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5690804.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            49184..49735
                     /gene="maa"
                     /locus_tag="OO230_000948"
                     /pseudo
     CDS             49184..49735
                     /gene="maa"
                     /locus_tag="OO230_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            49851..50321
                     /locus_tag="OO230_000949"
     CDS             49851..50321
                     /locus_tag="OO230_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5690805.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            50485..52035
                     /gene="pdeB"
                     /locus_tag="OO230_000950"
     CDS             50485..52035
                     /gene="pdeB"
                     /locus_tag="OO230_000950"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5690806.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(52077..52430)
                     /locus_tag="OO230_000951"
     CDS             complement(52077..52430)
                     /locus_tag="OO230_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5690807.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(52550..52646)
                     /gene="ffs"
                     /locus_tag="OO230_000952"
     ncRNA           complement(52550..52646)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO230_000952"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            52809..53120
                     /locus_tag="OO230_000953"
     CDS             52809..53120
                     /locus_tag="OO230_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5690808.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
BASE COUNT        12657 a        13983 c        13672 g        12818 t
ORIGIN      
        1 acctgccggt aaattgtgca gcgtatgttc gagtttatta ctcgccagta cctgttttaa
       61 ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg ccgcgaaaac cacactgcgc
      121 agctttctca aaacgggcga gaaaatcata ttcaccaaat aacatcgata aattagcaga
      181 gaaacgtaac atcatttatc tcccttattc atagtgcatg aagcaggttt cagtcggtgc
      241 atccgctgcg ttatcggcga tatcttcaaa ttccatgacg ttatccagtt cgctgcccat
      301 cgaaatattg gtcacacgct cgagaataac ttccacgact accggtaccc gatattgcgc
      361 cattaaggct ttcgcctgtt caaaggctgg cgcaatatct tccggtttga agacccgaat
      421 agctttacaa cctaaacctt ccgctacttt tacgtggtca acaccgtagc cattcacttc
      481 actggagttg atattctcga aagcgagttg cacgcagtag tccatgtcaa aagcgcgttg
      541 tgactgacga atcaggccga gataggcgtt attcaccagc acatggatgt acggaatgtt
      601 gaactgcgcg ccaacggcta actcttcaat caggaactgg aagtcaaagt cgccagaaat
      661 cgccaccaca ttgcgtttcg gatcagcggc acaacccccc agcgccgccg gaatcgtcca
      721 gcctaacgga ccagcctgac cacagttgat ccagtggcgg tctttaaaga catgcagcat
      781 ttgtgccgca gcgatttgtg acagaccaat agtggtgaca taacaaacat cgcgaccaaa
      841 ggctttgttc atctcttcat acacgcgctg cggtttcacc ggcacgttgt cgaagtgggt
      901 tttgcgcagc aaagtgcgtt tacgctgctg gcagtcggcg acccattctt tacgacacgg
      961 cagacgaccc gctttttgca tctcctgcgc cacttcaacc agcagtgtca gcgccgcttt
     1021 agcatcagag acaatgccga gatccggaca cagcacgcga ccaatttgcg tcggctcaat
     1081 atcaatatga acgattttgc gcccttcggt gtatttctct accgaaccgg tatgacggtt
     1141 agcaaaacgg ttaccgatac caaacaccat gtcggacgcc agcaacgttg cgttaccgta
     1201 acgatgcgcg gtttgcagac cgaccatccc ggccatcagt tcatgatcgt ccgggataca
     1261 gccccagccc atcagcgttg ggatcaccgg aacgctggtc agttcagcaa actgttgtaa
     1321 cagtgcagct gcgtcagcat tgattacccc gcccccggca acaatcaccg gacgttcggc
     1381 ctggattaac atttctacag ctttttcgat ctgcatacgg ctggcagcag gtttgtagac
     1441 cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc gcaacctgaa cgtcgaacgg
     1501 taaatccacc agtaccggac ccggacgacc agaacgcatc aggtgaaatg cctgttgcag
     1561 cacgcgaggc accagcgccg cttcacgaac tgtaaccgcc attttgctga ccggtttagc
     1621 aattgcttca atatctacgg cctgaaaatc ttctttatgc agacgggcgc gcggtgcctg
     1681 gccggtaatg cacagaatag gaatggaatc agcagaagcg gaatagagcg cggtgatcat
     1741 gtccgtgccc gcaggaccgg aagtccccag acatacgccg atattccctg ccgttgcgcg
     1801 ggtataacct tccgccatgt gcgaaacacc ttccacatga cgcgccagaa tgtgacgaat
     1861 accgccgtgc ttacgcatcg ctgagtagaa cggattaatt gcagctcccg gaacgccgaa
     1921 ggcggtggtg ataccttctt tctccagcac atacattgcc gcgtcaacgg ctctcatttt
     1981 tgccatttta ttcctacctc tattagttgg aaaaattttc caactttcaa attttgtcaa
     2041 aaaaacccgc tccgcagagc gggatgcgac agacactatg gatgtgcttt cagtcccaac
     2101 gccgtgctga tatcgcgggc ggtgtctctg accagctcac cctgactgac aaaacgatct
     2161 tctgtcagtc ttgatgaagg cccggagatg gagatagcgg caacaactct accgacatca
     2221 tcgtaaattg ctgaagctat gcaattcaga cctacaacat gctcttcttt atctacggta
     2281 tagcccagtt cacgcgcttg ttccaggtcc ttcagcaagg tgggcatatc cacaagcgta
     2341 gttggcgtaa actgctgcaa accggtttgc agaatgatgc tcatcaactc ctcttccgcc
     2401 agcggataaa gcagcgcttt gcccgcaccg gaagcatgca gcggcagacg actgcccagt
     2461 ggcgcacaca tcctgaccat cgatttacac tctaactgac caattaatac cgcttcattg
     2521 ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta acatcaggcg gcgcataaac
     2581 ggcccggcga cggagaggac atcgcggtta tggatgtacg ccgcaccgac gttaaagaca
     2641 cctaatccta tatgccacca gcctaattga ctgtcctgat agacaaaatc cgctgcttgt
     2701 aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat ccagattgag agaaatatcg
     2761 ctaaccgacg aacttccccc acttttttcc agatattgca gaatcgcaat tccccgctct
     2821 aacgcctgtg cgcccttctg tgccacaggc tccgcctgtc ctggcctgcc gcgccgtcta
     2881 acttccgtca tacctacgtt aactccgacc tacaagtgag cataaacaat gaacaattag
     2941 tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca gagttcctgt tcaggaatac
     3001 tttcaacatc acagttgtca ctgccgccgc gatcgatggt cagaaaatcg gtgacgcgct
     3061 gccaggcgaa aagtgggtga tgccagacgt tacgatggta attcaccccc tgttcaccgt
     3121 tggtgataaa cgcccgcagc gttgacaggt ctggtttgtc gtcgcctaac gccacgacca
     3181 ccacaaacac ttcacctttc attgggataa aggcctgagt gcccagcgga tgacgttcga
     3241 gttcgtgaat ggtcagcggc agattcgccg gttgcgcgcg gttaatgctg ataagcgtac
     3301 ggtcttgctc aagaatctca accagcgcca aatcgtggta acgctccacc aggccattgt
     3361 taatatggaa aaaatcccgt tgctgcgttt cgattacgtc gccataagca ctaaaggctt
     3421 cctgacttaa cggtaatacc tgaagtttca tctttttgtc cttgtaacat aattccgttc
     3481 aaccaccgcc gaccggtcag cttggtttca ataacaggca atctaaaacc ttaagagaac
     3541 aacattaata caaaagttgg aatgtttttc taaaactaga tttagtccac aaattgaaca
     3601 ggattatcat gtaaaaatac tgataaatca gaggataaga aaatcacgca acttgcggat
     3661 ttaaaagggg atacctgaag atgttcgatc cagaaacctt gcggactttc attgcggttg
     3721 ctgaaacagg aagtttttca aaagcggcag aacgattatg taaaaccacg gcgacgatca
     3781 gttatcgcat taaacttctg gaagagaata ccggagtagc gctgtttttc cgtacgactc
     3841 gtagcgtaac gttgacagcg gctggcgagc atctactttc ccaggccaga gactggctga
     3901 gctggctgga aagtatgccc agcgagctgc aacaggtgaa tgatggcgtg gaacgccagg
     3961 tgaatattgt catcaacaac ctgctctata acccccaggc cgtcgcccag ttgctggcgt
     4021 ggctgaatga gcgttacccc tttacccagt ttcacatctc ccgacaaatc tatatgggcg
     4081 tctgggactc gctattgtac gaaggttttt cgctggctat cggggtcacg ggaactgagg
     4141 cgctggcaaa tacctttagt cttgatccct taggatcggt gcaatggcga tttgtcatgg
     4201 cggcggatca tccgctggcg aacgttgaag agccgctaac agaagcgcag ttgcgtcgct
     4261 ttccggcggt caatattgaa gacagcgccg gcaccttaac caaacgcgtc gcctggcgat
     4321 tgccggggca aaaagagatt attgttcccg atatggaaac gaaaatcgcc gcccatctgg
     4381 cgggtgttgg cattggtttt ttgccaaaat cactttgcca gtcaatgatc gataatcaac
     4441 aactggtcag ccgggtaatc ccaacgatgc gccctccttc gccattgagt ctggcatggc
     4501 gcaaatttgg cagctgcaaa gcggtagaag atattgtgac cttgtttacc caacacaggc
     4561 cggaaatcag cggattttta gaaattttcg gcaccccacg cagttaaaaa tcaagtttcc
     4621 ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga ggttcacttt taactatgca
     4681 agagagacac acggaacagg actatcgtgc cctgctgatt gctgatacgc ccattattga
     4741 tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc gccgctatca atctgccgtt
     4801 aatggataac gatgaacgcg cctccgttgg cacctgctat aaacagcaag gctcagacgc
     4861 agcgctggcg ctggggcata aactggtggc gggtgaaatt cgtcagcagc gtatgtacgc
     4921 ctggcgggca gcgtgcctgc aaaatccgca aggtattctc tgctgcgccc gtggcggtca
     4981 acgttcacat attgtgcaac gctggctgca tgaagcgggg attgattatc cgctggtgga
     5041 aggcggttat aaggcactgc gccagaccgc gattcaggcg accattgaac tggcacaaaa
     5101 accgatagtg ctgattggcg gttgtaccgg cagcggtaaa acgctattag tgcaacaaca
     5161 gccaaacggt gttgatctgg aagggctggc gcgtcatcgc ggttcggcgt ttggtcgcac
     5221 gttacaacca caacttagcc aggcgagttt tgaaaacctg ctggctgccg aaatgctaaa
     5281 aaccgacgcc cgtcaggatt tgcgcctgtg ggtgctggaa gatgaaagcc ggatgatcgg
     5341 ttcgaatcac ctgccggaat gtctgcgcga gcgaatgact caggcggcga ttgcggtggt
     5401 agaagatccg tttgagatcc gtcttgaacg cctgaatgaa gagtatttct tgcgtatgca
     5461 tcatgatttt acccacgctt atggcgacga acagggctgg caggaatatt gcgaatacct
     5521 gcatcacgga ctttcggcga ttaagcgtcg gctggggtta cagcgctata acgaactcgc
     5581 tgcccagctg gatacggcgc tgacaacgca actcaccacc ggcagcaccg acggtcatct
     5641 ggcctggctg gtgccgttac ttaaagaata ttacgaccca atgtatcgct atcagcttga
     5701 gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg gaagtggcgg tatgggttaa
     5761 ggcgcagtga tgggcggtat tctgaaacgg cgattgtcgt gagaacaaaa tggttgcacc
     5821 agaatacaga tataaaaaaa ccgacatgac agtcggtttt tttatttttt ccgcccaaga
     5881 cttggtggtt ccgggcagaa attaaacagt cctcatctag caagtgaatt ataagtcagg
     5941 atgcctacat attcaaacag ctatgttggc gatacaatcc cgacgctagt agtgatactg
     6001 cctcaaggga ttaccgcagt gcggaaaagt acttttaagt cctggtcagg atacaggtgg
     6061 tgctgattct ggtgctctaa tttggagaga gacagtcctc atctagcaca tgaagaaaat
     6121 catagttata ggcaacgtaa gggtaagctg tgattgtctt tctcccttaa gggagcaacg
     6181 gatgaaaact tttctcgata aagttctcat cgttcttaag gcgctaatcg cgctgctgga
     6241 actgatccgt cagtttattg aataactgag gaaacgtagc taaggccgaa ggtttacctc
     6301 cttcggcctt taaaaaaacc gacatgacag ttggtttcat atttattgcc aaaatgcaca
     6361 ctgttcatgc cagatgcggc gtaaacgcct tatccggcct acacaatctt gcaaaatcaa
     6421 tatattgcac gctcttcgta ggcctgataa gcgtagcgca tcaggcaatc taacacctgt
     6481 catcacccca caaactgcct gaacagcgcc ttacccttaa ccagtcgcgc acccagccag
     6541 ccgccgaaaa gcgacagcag cagcgcaccg ctgcacggca gaacaatcca caatcgccag
     6601 tctggctccc acgggaaatc aaacactttc gcctgcaaca ctgccagtgc cgtttccgca
     6661 ccaattgcgg ccaccaggcc ggaaacaaac ccaagcatgg cgaacatcac accacaatgt
     6721 cgtgcgcagc agttttttcc ccgcaccgag tgtgcgccat accaccagct cctgatgacg
     6781 ctggcgcatt cccacctgca cctgtgccag caacagcaac ataccgcagg cggtgaccag
     6841 caccaccatc acctccagcg cccgacttac ctgctccagc acctgaccga cctgttttaa
     6901 aatcgcgcca atgtctaaca ggctaatggt cgggaactgg cggttgagtt gcgtcaacat
     6961 gccgttgcca ttctcccagc ggaaactggt aagccagctc tgcggttgcc cgtctaatgc
     7021 cccttcaggg aaaataaaat agaaattagg ccgcagactt tcccagtcca ctttgcgcag
     7081 gctggtcact ttagcgcgga actcctgggt atcgcccata aaagtcacgg tatcaccgag
     7141 ggcaacgttt aagcgtttcg ccagcccctc ttccatcgac acttcatcgg cttttggcgg
     7201 ccagttaccg gcgacaatcg gattatgatc gggccgcgta ttttgccagg taagattgag
     7261 ttcgcgatta agcgcctcat cttcattacc ttctgtcggc ttatcattaa tcgccgtcag
     7321 ccgcgcccgc actaccggat aaaatgattc cgggactata tgatgttccg cgaggaacgc
     7381 ttttagcggt gctacctgtt ctgtggcgat gttaattaaa aagtagttcg ggctttctgg
     7441 cggtagctgc tgctgccagc ggtcgagcag atcgccacgc agcaccagca gcagtgccag
     7501 cagcataaag gagagcgaaa atgccgaaag ctggcttaac gttgaccacg gctgacgtaa
     7561 caggcggcta accgccaggc gcagaggcag cgatttcagc gtcatgcggc gaagtacatt
     7621 cagcagcatc cagcccagca caccgcacag caaagccagt actaccgcgc ccgccagcac
     7681 cgcccaaagc agcatgctgc cacccattaa tccggcgagc agcagcacaa ccaccacact
     7741 gacaatcggc agataaaact tcagcggcca gacgttcgcg accacatcat tacgtaatac
     7801 gcgtaaaggc tgcgttgcca gcaacaagcg gtatggtcgt agccccacca gcagcgagat
     7861 gaccgtcatg gtgccaagcg cccacagcca cggccagagg ctggctggcg gtagtgcagc
     7921 aggcagaacg ggcttgagca gcaccatcaa cacgttttcg aacaacaggc ctatggcccc
     7981 accggtaacg gctgaaagcg tcagcaccat caaccactga ccgacgatta gcttacgcag
     8041 ttgcgctcgc cctgccccca gcgttttgag gatcgccacc agatcgtagc gactgcgaca
     8101 gtaatgattc atcgctaccg ccaccgctgc cactgccagc agcaaggtca gaagcgccga
     8161 aagcagcagg aactgttgcg agcgttccat cgatcgcccc agcgcgcctt cgtcctgttc
     8221 cagaccgtac cagcgttgtt cgggtttaag ctgaggtaac aaccatttct catagccgtc
     8281 gagctggttc tcgttgccgc cgaatttata acgccaggtg acccgactcc ccggctgcac
     8341 ggctccggtt ttatcgacat ccgccagatt catcatcaga cgcggagcca tctggaaggg
     8401 gttaaaaccg gaatccggtt cctgaatcac ttctccggca atccgcaagg tggcatcgcc
     8461 cacgtcaatg gtgtcgcccg ttttcaggtt aagcagtgcc atcaggcgtg gggccagcaa
     8521 tacgctgccc gcctgcggtt tcaggccagg gggattagtt tgcagaccgc catacatcgg
     8581 gtagatatca tccaccgctt tgacgttcgc cagctgcggt gtgtcgcctg caaaggtcat
     8641 tgtggcgaaa gtcagctgct tgccgacttt caggccgcgc ttttgcgctt cctccagcca
     8701 cgcttgcggc acttcgcgtg aactgcgcaa cgcccgatcg cccgccataa actcacggct
     8761 ttgctggctt aagcccttct ccatgcgatc gctgatattg cccagcgcca gcacgcaggc
     8821 caccgccagg cttagcgcca gccagacaat taatagcgac ggcgaacgcc attcgcgcca
     8881 gaaccaacgt gcaatcatgc ttcctcctgc aactgcccgt tcaccagccg taagcagcgg
     8941 tcgcagcgtg ccgccagttg caggtcgtgg gtcaccataa tcaacgtggt gccatgttca
     9001 cggttgaggg aaaacagcag gtcggcaatt ttatcgcccg tctggcggtc aaggttgccg
     9061 gtgggttcgt cggcaaacag cacatcaggt cgaccattaa aggctcgtgc cagcgccact
     9121 cgttgctgtt caccgccgga aagctgtgcc ggaagatgat ccagacgttt acccagccct
     9181 aactgttcga gcaacgcttt cgccccgtta cgactttccg cgctactctc accgcgcagc
     9241 agagccggaa gctcgacgtt ttccagcgcg ttaagggtag gaattaacat aaatgactga
     9301 aaaacaaagc cgacgtgctt cgcgcgcaac tttgcccgcg cttcttcgtc catattatgt
     9361 agcggttgtc ccaccagact cacttcgcca ctgctgccgt catcaagccc ggcgaggatc
     9421 gccagcaagg ttgacttacc cgatcccgac tcgcccacca gtgcgatggt ctcgccacgt
     9481 ttgacaacca gctcaactcc ggtgaggatg gagagttcat gctccccctg accgacggac
     9541 ttcttaagat gatgaacttc aacaatgttt tccgctggca tttgcccttc ctgttcctgg
     9601 tcctgttaac cttccgtgcc gccgcagcgg acacgttatt gattctgggt gatagcctga
     9661 gcgccgggta tcgaatgtct gccagcgcgg cctggcctgc cttgttgaat gataagtggc
     9721 agagtaaaac gtcggtagtt aatgccagca tcagcggcga cacctcgcaa caagggctgg
     9781 cgcgccttcc ggctctgctc aaacagcatc agccgcgttg ggtgctggtt gaactgggcg
     9841 caatgatggt ttgcgtggtt ttcagccaca gcaaaccgag caaacgctgc gccagatttt
     9901 gcaggatgtc aaagccgcca acgctgaacc attgttaatg caaatacgtc tgcctgcaaa
     9961 ctatggtcgc cgttataatg aagcctttag cgccatttac cccaaactcg ccaaagagtt
    10021 tgatgttccg ctgctgccct tttttatgga agaggtctac ctcaagccac aatggatgca
    10081 ggatgacggt attcatccca accgcgacgc ccagccgttt attgccgact ggatggcgaa
    10141 gcagttgcag cctttagtaa atcatgactc ataaagcaac ggagatcctg acaggtaaag
    10201 ttatgcaaaa atcggtctta attaccggat gttccagtgg aattggcctg gaaagcgcgc
    10261 tcgaattaaa acgccagggt tttcatgtgc tggcaggttg ccgaaaaccg aatgatgttg
    10321 agcgtatgaa caacatggga tttaccggcg tgttgatcga tctggattca ccagaaagtg
    10381 ttaatcgcgc agcagacgag gtgatcgccc tgaccgataa ttgtctgtat gggatcttta
    10441 acaatgccgg attcggcatg tatggccccc tttccaccat cagccgtgcg cagatggaac
    10501 agcagttttc cgccaacttt ttcggcgcac accagctcac catgcgcctg ttacccgcga
    10561 tgttaccgca cggtgaaggg cgtattgtga tgacatcatc ggtgatggga ttaatctcca
    10621 cgccgggtcg tggcgcttac gcggccagta aatatgcgct ggaggcgtgg tcagacgcgc
    10681 tgcgcatgga gctacgccac agcggaatta aagtcagcct gatcgaaccc ggtcccattc
    10741 gtactcgctt caccgacaac gtcaaccaga cgcaaagtga taaaccagtc gaaaatcccg
    10801 gcatcgccgc ccgctttacg ttgggaccgg aagcggtggt cgacaaagtg cgccatgctt
    10861 ttattagcga gaagccgaag atgcgctatc cggtaacgct ggtgacctgg gcagtaatgg
    10921 tgctcgagcg cctgctgcct gggcgcgtga tggacaaaat attgcagggg tgagttgaag
    10981 cgcgcgctta agcccccatg tcaaagaaaa tatcgacaac agagagcgac tccatgtccg
    11041 tagaaaatat tgtcaacatt aacgaatcta acctgcaaca ggttcttgaa cagtcgatga
    11101 ccactccggt gctgttctat ttttggtctg aacgtagcca gcactgtttg cagttaaccc
    11161 cgatcctgga aagcctcgcg gcgcagtaca acgggcaatt tattctggcg aagctggact
    11221 gcgacgcgga gcagatgatt gccgcccagt ttggtctgcg cgcgattccg accgtgtatc
    11281 tgttccagaa cgggcaaccg gtagatggct tccaggggcc gcaaccggaa gaggcgatcc
    11341 gcgccctgct ggataaagtg ctgccgcgcg aagaagagct gaaagcgcag caggcgatgc
    11401 aactgatgca ggaaggcaat tacaccgatg ccctgccatt gctgaaagac gcctggcagt
    11461 tgtcgaatca gaacggggag atcggcctgc tgctggcaga aacgctgatt gcgctgaacc
    11521 gttctgaaga tgcggaagcg gtgctgaaaa ccattccgtt gcaggatcag gacacccgct
    11581 accaggggct ggtagcgcaa atcgaactgc tgaagcaggc ggctgataag ccggaaattc
    11641 aacagttgca acagcaggtg gcggagaatc cagaagatgc cgcactggcg acgcaactgg
    11701 cgctgcaact gcatcaggtt gggcgcaatg aagaggcgct ggagttgctg ttcgggcatc
    11761 tgcgtaaaga tctcaccgcc gcagacggtc agacgcgtaa aacgttccag gagatcctcg
    11821 ctgcgctggg tacgggtgat gcactggcgt cgaagtatcg ccgccagctg tatgcattgt
    11881 tgtattgata ttccgttgtt cggcacatga accacaacgc cggatgcgat gctgcgcatc
    11941 cggcatcgca tcatttcttc ttcaattgag tcaccaccaa ctggtggcgc gaattataaa
    12001 acttacgata ggttaaatag caggcaataa tggtcgacaa gctggcggtt gagagcagca
    12061 taaaggtcac cataatctga tatttaatcg ccttcaccgg atcaatcccg gcaaatatca
    12121 gcccggacat cattcctggt aaactcacta agccaaccgt ttttgctgaa tcgaccgtcg
    12181 gaattaaagc cgcgcgaata ctgtcgcgaa tcaatatcgc tgaagcctgc ttcggcgtcg
    12241 cgccaagact cagtttctcc tgaatctgtt gctgttcgct aatgacccgt tgccctaaat
    12301 tgttgtaaca caaccccacc gctaccatgg cgttaccggc aatcatcccg gcgacaggga
    12361 tcacctgcat cgggataaat tcaatcgccc ctgagagaat cagcaccgcc agggtaattc
    12421 ccgccccgac cgtaatggca ataaacgatg agataaaagc tttagcaata tatttactgc
    12481 gtttttgcgc gttccacgcc gcattaaagc agataaataa caccatcaat aatgtcaggc
    12541 tggcatcatc cacgctgaaa atatacttca gcacatagcc gacaataatc agctgaatta
    12601 tcgctcgccc gacgctccat agaatatctt tctccagcgc cagtttttct ttatggctaa
    12661 ttaagattgc caccaccacc agcattaatg ccagtgctaa tgattcgtta gtaatattat
    12721 gcgagttcat agcgtgcttc ctgcatttct ccggcatgcg gttgcagtgt aatcacttta
    12781 tccgcatgat taatttcgtc tttatcgtgt gtcacccaca gcacggcaat attttgctcg
    12841 cgcacataac gatggatcat ctcattgacg ttatgtttat tactttcatc cagcgcactg
    12901 gttatttcat ccagcaataa aaccttcggc ataaattgca ggttacgaat caatgagatg
    12961 cgttgttttt caccaccaga tagctcggcg atattcttcg tcaaaatgct gtccggcaaa
    13021 gcgaagcgtt cgagaaaatc gagaaaaatg gccgggtcag gctgctggtc acggatctgc
    13081 cagggaaaga tcagattatc gtataccgta tcgccaaaca gcgtcggcgt ctgggcgcag
    13141 taagagactt gctggcggta gatttctggt tttagtgtgc tgacatcctc accttcaaac
    13201 agtaacgttc cgctggttgg gctgatcaat gaagcaacta tttttagcag cgtacttttg
    13261 ccacaaccag aaggaccggt aattaactta aattcgccag cacgcagcga aaaattgatg
    13321 ttattaagaa tcttcgtatc acccgccaga tatcctacgt tttgtagctg aagcaaagga
    13381 ctattttcct gcatcgctgt tccctttttc tgatttttac taaaaacagt ttatccttcg
    13441 caggaataag ggggaactct ctttcagtaa tcaggtataa tttgtttaat caagcagcaa
    13501 ttttgctgat tatggacggt acaacaggag gtttttccga tgcttatctt tatcccgatt
    13561 ctcatttttg tcgcgctggt cattgtcggc gcgggcgtca aaatcgtgcc gcagggctat
    13621 caatggacgg tagaacgttt tggtcgctat accaaaacgt tacagccggg gctcagtctg
    13681 gtggtgccgt ttatggatcg cattggtcgc aagatcaata tgatggagca agtgctcgat
    13741 atcccttccc aggaagttat ctcgaaagat aacgccaacg ttaccatcga cgcagtctgt
    13801 tttattcagg tgattgacgc gccacgcgct gcctatgaag tcagcaatct ggagctggcg
    13861 atcatcaacc tgaccatgac taacatccgt accgtgttgg gttcaatgga acttgacgaa
    13921 atgctctctc agcgcgacag catcaactca cgcctgctgc gtattgtcga tgaagccacc
    13981 aacccgtggg ggattaaagt cacccgtatt gaaattcgcg acgtgcgccc accggcagag
    14041 cttatctctt caatgaacgc gcagatgaaa gcggaacgta ccaaacgcgc ttacattctt
    14101 gaagcggaag ggatccgtca ggcggaaatc ctcaaagccg aaggtgaaaa acagtcgcaa
    14161 atcctgaaag cggaaggcga acgtcagtcg gcgtttttac aggctgaagc gcgtgaacgt
    14221 tccgccgaag cagaagcccg cgccaccaaa atggtgtctg aagctatcgc ctccggtgat
    14281 attcaggcgg tgaactactt cgtagcgcaa aaatacaccg aagcgttaca gcagatcggt
    14341 tcctccagta acagcaaagt agtgatgatg ccattagagg ccagcagcct gatggggtcg
    14401 attgccggga ttgccgagct ggtgaaagac agcgccaaca aacggactca gccatgatgg
    14461 agttaatggt cgttcatcca catattttct ggctcagtct cggcgggttg ctgctggcag
    14521 ccgagatgct gggcggaaat ggttatttgt tgtggagcgg cgtggcagcg gtgattaccg
    14581 gcctggtggt ctggctggtg ccgctgggtt gggagtggca aggggtaatg tttgccatcc
    14641 tgacgctgct cgccgcctgg ctgtggtgga aatggttgtc gcggcgggtg cgcgaacaaa
    14701 agcacagcga tagtcattta aaccagcgcg ggcagcagct gattggccga cgttttgtgc
    14761 tggaatctcc gctggtcaac gggcgcggtc atatgcgcgt cggtgacagt tcatggcctg
    14821 tcagcgccag cgaggatctc ggtgcaggta cgcatgttga agtcattgcg atagaaggga
    14881 taaccctaca tatccgagcc gtctcgtctt aatcaccctg cccgatgatg acagcagccg
    14941 gagagatttt cgataatcgg gcagtcggcg ctgtcatcgc cagggcaggc attcgccagt
    15001 gccagcagct ggtcgcgcat ggattgcagc tcctcaatgt gtcgttcgat ctccgccacc
    15061 ttctccagcg tgcgccgttt gacgtcggcg ctgtgccgct gcgggtcgtt aaacagattc
    15121 accagctcgc cgctctcttc caggttaaag cccacctgcc gtgcctggcg cagtaaggtc
    15181 agttcgttga gatgctgctg cgtgtaggtg cgataaccgt tttcgctgcg catcggcggc
    15241 gtcaccagcc ccttctcttc atagaagcga atggctttgc tggtcaggcc ggtaattttt
    15301 gctacatcgc tgatgttcat cgttcgcgca acgcctcctg tgctttgtta aagggtttca
    15361 acagatattc aagcacgttt ttttgccagt gcgaatatcg accgccacca caataataca
    15421 ggaagtactg cgcacagaga ggtgttatac ggttttatta cgcttcatga ccattgccac
    15481 aataaggctg agtaagattg caccaataat ccatatcaac gcacggctgc cctgggacca
    15541 caggctgtag ataaagccaa cgatcaccaa caacatcacc aacgtaccaa cgataagaat
    15601 cggcaacgag gcgtgaatat catgacgcag gcggatcgcc accacaaaca ccgccagata
    15661 gcagatcaaa aaggtggcgc tggcaacgct ggcgagtgag tctaaattta gcgccgccgt
    15721 catcagcatt atcaacacca cgacgatgat attgccccag gtactctgcc gccacaggga
    15781 tttattcatt agcttcggca gttcgcgttc gctgcccatg ttgtccatga tgttaaacac
    15841 ggcgaacagg ttcgcgttaa tgcctgaagc cgtcgccagt aaagcgccga tgacgacgat
    15901 cacataaccc acatgcccga gcagcggaga agcagcctgc gctacggcgg tatcagcata
    15961 tttttctaac tctaatgccg atacatcgct aagcaaaacc agcgccagcg agatataaag
    16021 caacgtggta acgccaatcg ccaccagaaa cgcccgtggc ataatgacct gcggatcttt
    16081 cactttatcc gccgcgttcg ccatcatgcc aaagcccgca taggcaagga aggttatccc
    16141 aatacaggag aagaacgcac cggagctggg gggcgcagag acggaaatat gcgccggttg
    16201 cagcgaccag acaccggcaa taatcagcaa taacaggatc atcattttaa tgccgacgag
    16261 gatcacttcc agccgcccta ccgcatggtt gcttaaggag ttgaaaagcg tcatcaccgc
    16321 aatgatcccc aacgcgtaga gcaaaataag gtgctcctcc tggctgcctt catgcaaaaa
    16381 ctgcacggca taagcgccaa aagcacgggc aaccatggcg atgctcaccg ccagcgtcaa
    16441 caggtatagc aacgagagcg ctagcgaaaa gacgccgttg cctaatccgc gacgaaaaaa
    16501 gtcgataata ccgccattgc tgggatagct cgcccccaga cgcgcatagg cataaccgga
    16561 aaacatagcc acaataccgc caaaagcaaa ggcgccccag gtcgaggctt ccattagcaa
    16621 cgcagcctgc cccagcagcg cgaagatccc cgcccccacc attgccccaa taccgatgga
    16681 aacgacgttc catagaccga ggggtttgtt accgttatta ccttccgtgt tcatcatgat
    16741 cagcccttaa acacgttata gccgagttgc ttagcgaccg atgccaccat tttttgaccg
    16801 cgaacactat tgccttcttc gtccagcggt ggtgagaacg cggcaattcc catcacgcca
    16861 gggacgaccg ccagaatacc gccacctaca ccgcttttgc ccggtaaacc aacgcgatac
    16921 gcccagtcac cggagcgacc atacagccct tccatcatca tttcggccag aatgtacggc
    16981 acgttgtcgg cctgaagaac gcgtttatgc gtcaacggat tcacaccacc cgccgccagc
    17041 gtcgcgccaa gcgttgccag ttcaacagta ttgatgagcg tagagcactg acgggtgtac
    17101 acgtcacagg cttccattgc atcacaatag agatatccgg cggagtacag cagccaggca
    17161 atggcccgat tatggaagtt ggttgtttgt tccgactggt tgacttcgtc agagagcgct
    17221 acctgctcac cagccagttg ctgttggata tgtaaaattc gctgccagcg ttgttcggcg
    17281 ttttcagcgt taatcaggct ggtggtggca atagcgccag catttaccag tggcgaaagc
    17341 ggtttgccgc catgcaactc taaggcgata actgagttaa agggcaatcc ggtcgggtca
    17401 gcgccaattt tgtcctgtac cgcctgcggg ccgacatctt ctaacgcaag ggctaacgta
    17461 cagactttag agatggattc cagtgcaaag cggtaatcac tgtcacccgc actatagacg
    17521 ttgccatcgc aggtcacgat agccactgcc gccagttgac ctggtacatt cgccagaaag
    17581 ggaatgtaat cggcattttg tccgccgtta agtgagtgaa attgggtgta agcctgatcc
    17641 actgcctgct gtaatttgtt tgcatctaac atcttttgtt aactcctttt tatagatgcg
    17701 ggaggtaatt cctcacccca gtgccgattt tcaggcatcc tgatttaact tagcacccgc
    17761 aacttaacta caggaaaaca aagaaataaa tgtctaatcc tgatgcaaat cgagccgatt
    17821 ttttaatctt tacggacttt tacccgcctg gtttattaat ttcttgacct tccccttgct
    17881 ggaaggttta acctttatca cagccagtca aaactgtctt aaaggagtgt tttatgtcac
    17941 aaactatcga cctgaacctg gacggcctgt cctgcggtca ctgcgttaaa cgcgtgaaag
    18001 aaagtcttga acagcgcccg gatgtggaac aagcggatgt gtctatcact gaagcgcacg
    18061 ttaccgggac tgccagtgca gaacagctga tcgaaaccat caaacaagcg ggttatgacg
    18121 catctgtaag ccacccaaag gctaaaccgc tggcggagtc atcaatcccg tcggaagcac
    18181 tgacagcggt ttctgaggcg cttccggcag cgaccgccga tgacgatgac agccagcagt
    18241 tgctgctgag cggcatgagc tgcgccagct gtgtcacccg cgtacaaaat gcgctgcaaa
    18301 gcgtaccggg cgtcactcag gcacgggtaa acctggcgga gcgtactgcg ctggtgatgg
    18361 gcagtgcctc cccacaagat ttagtgcagg cggtggaaaa agcgggctac ggcgcggaag
    18421 cgattgaaga tgacgcgaaa cgccgcgaac gccagcaaga aaccgccgtc gctacgatga
    18481 agcgcttccg ctggcaggca attgtcgcac tggcggtggg tatcccggtg atggtctggg
    18541 ggatgatcgg cgataacatg atagtcaccg ccgacaaccg cagcctgtgg ttggttatcg
    18601 gcctgataac cctggcggtg atggttttcg ccagcggcca tttttaccgc agtgcatgga
    18661 aaagcctgct gaacggtgca gcgacgatgg atacgctggt ggcgctggga acgggcgtgg
    18721 cgtggctcta ttcgatgagc gtcaacctgt ggccgcagtg gttcccgatg gaagcgcgac
    18781 atctttacta tgaagccagt gcgatgatta tcggtctgat caatctcggc catatgctgg
    18841 aagcacgcgc acgtcagcgt tcttctaagg cactggagaa attactcgat ttaaccccac
    18901 cgacggcgcg cctggttact gacgaaggtg aaaaaagcgt gcctctggca gaagtgcagc
    18961 caggtatgtt gctgcgcctg acgaccggcg atcgcgtgcc ggtagatggc gagattaccc
    19021 agggcgaagc atggctggat gaagcgatgc tgacgggcga accaatcccg cagcaaaaag
    19081 gcgaaggcga tagcgtccat gccgggacag tggtacagga cggcagtgtg ctgtttcgtg
    19141 ccagtgcggt tggcagccat accacgctat caaggatcat tcgcatggtg cgccaggccc
    19201 agagcagcaa gccagaaatc ggtcagctgg cggataaaat ctcagccata tttgtgccgg
    19261 tagtagtggt tattgcgctt gtcagtgcgg caatctggta tttctttggt ccggcaccgc
    19321 agattgtcta taccctggtg attgccacta cggtgctgat tatcgcctgc ccgtgtgcgc
    19381 tgggactggc gacgccgatg tcgattattt ccggcgtcgg gcgggcggct gagtttggcg
    19441 tgctggtgcg ggacgccgac gcgctgcaac gcgccagtac actcgatacc gtcgtgttcg
    19501 ataaaaccgg gacgctgacc gaagggaagc cgcaggttgt cgcagtgaaa acatttgctg
    19561 atgttgacga agcgcaggcg ctgcgtctgg cggcggcgct ggagcaaggt tccagccatc
    19621 cattggcacg agcgatcctt gataaagcag gcgatatgca gctaccgcag gtcaacggtt
    19681 tccgcacatt gcgcgggctg ggcgtgagcg gtgaagcaga aggtcatgcg ttattgctgg
    19741 gtaatcaggc gttgttaaat gatcagcaag tcgatactaa agcgattgaa gccgatattt
    19801 cagctcaggc atcgcaagga gcaacgcctg tgctgctggc gattgacggg aaagcagtag
    19861 ccctgctggc tgtacgcgat ccgttgcgta gtgatagcgt ggcggcgctg caacgcctgc
    19921 ataaagcggg atatcgtctg gtgatgttga ccggggataa cccaaccacc gccaatgcga
    19981 tcgccaaaga agctggaatt gatgaagtga ttgccggagt gctgccggac ggtaaagccg
    20041 aagcgatcaa acgtctgcaa agtgaaggac gtcaggtggc aatggtgggc gacggcatta
    20101 acgacgcgcc agcgctggcc caggcggatg tcggcattgc gatgggtggc ggcagtgatg
    20161 ttgccattga aaccgcggcg attaccctga tgcgccatag cctgatgggc gttgcggatg
    20221 cgctcgcgat ttcccgcgca acgctgcgca acatgaagca gaacctgctc ggtgccttta
    20281 tctacaacag catcggtatt ccggtcgccg ccggtatttt gtggccgttt actggaacac
    20341 tgcttaaccc ggtagttgcc ggagcggcaa tggcgctctc gtcaattacc gtagtaagta
    20401 acgccaaccg gttgctgcgg tttaaaccga aggaataagt acgtcattgc ggatgcgctg
    20461 ctttagggcg gcgcatttgg catcgacgca tgactgccgg atgcggcgta aacgccttat
    20521 ccggcctacg gttcaggcac aggtttgtag gcctgataag acgcgtaagc gtcgcatcag
    20581 gcaatggtgc tcggatgccg gatgcggcgt gaacgcctta tccggcctac gagatgaagc
    20641 gatgttttgg ccttacgcga ccttttttgc acgctcttca cggcgtgcca aatattcagc
    20701 cactgtttca atccgtccca attcttcctg ggtgcgcatg ttgttttcaa cttcccaaag
    20761 atcaactgcc gcctggaggt ttagcctaaa atcgactgtg gtatcaaaaa ctttcgccag
    20821 acggaatgcc atctcagtgg tgagtttacg attgttattg atcagtgcac tgacgcgatt
    20881 acgatgaaca tgcagcaact ctgctaactc attgattttc aaatcgagcg gttccagata
    20941 ttcatagaga agaatatctc cgggtgtcgt cggttttctt gttgcctgtt tcatggtttt
    21001 taactgttgc ttccctgtga atagcgatgc aagaacatac tgaatcatac aaacacatta
    21061 aacaagtccc caactccatt tgcgccatcc ctcaccgcgc gctagcatga tatttcacaa
    21121 agggaggtcg tatggatctg ttgtaccggg taaaaacgct ttgggccgcg ctgcgcggta
    21181 atcattacac ctggcctgcc atcgatatca ccctccccgg caatcgccat tttcatctga
    21241 ttggcagtat tcatatgggt agccacgata tggctcccct gcccacccgt ttgctcaaaa
    21301 agctcaaaaa cgccgatgcg ctgatcgtcg aggcggatgt ttccaccagc gatacgcctt
    21361 ttgctaattt gcctgcctgc gaggcgctgg aagagcgcat tagcgaggag caactccaaa
    21421 acctgcagca cattagccag gagatgggca tttctccctc actcttttct acccaaccgc
    21481 tgtggcaaat cgcgatggtt cttcaggcga cgcaggcaca aaaactgggg ctgcgggcag
    21541 aatacggtat cgattaccag ctattgcagg cggcgaagca acaacataaa cccgtgattg
    21601 aactggaagg ggctgaaaac cagattgcca tgttgctcca gctccctgac aaaggactgg
    21661 cgctgctgga cgatacgctg acccactggc ataccaacgc acggttgctg caacaaatga
    21721 tgagctggtg gctgaatgca ccgccgcaaa ataatgatat aacgctgccc aatacgttca
    21781 gtcagtcgct gtacgatgtg ctgatgcatc agcgaaatct cgcctggcgg gataaattac
    21841 gcgccatgcc gccggggcga tatgtggtcg cggtcggtgc actacacctg tatggagaag
    21901 ggaatttgcc gcaaatgttg cgctaaaaaa atggccaata tcgctattgg cccgtcaaag
    21961 aggaatttca tttttttatt attatgccgt cactttaagc gacggtgtaa ctcgattgtc
    22021 gctcaatctc accatcctgc caatactctt aggcaagatg actctttttt ttggaccgcc
    22081 atcaggcgta tgcttgcttc gtttttttgt ttaaggcagg aaaattacca tgacacccgc
    22141 agttaaatta ctcgaaaaaa acaagatttc gtttcaaatc catacctacg agcacgatcc
    22201 ggctgaaacc aattttggcg atgaagtcgt caaaaaatta ggtttgaatc cggatcaggt
    22261 ctacaaaacg ctgctggtgg cagtgaacgg tgatatgaaa caccttgccg tggccgttac
    22321 gccggtcgca ggtcaactgg atcttaaaaa agtagcaaaa gcgctgggtg ccaagaaagt
    22381 tgagatggcc gatccgatgg tcgcgcagcg ttcgacggga tacctggttg gggggattag
    22441 cccactgggg caaaaaaaac gtctgccaac gattatcgac gcccccgcac aagaatttgc
    22501 cactatttat gtttccggcg gcaagcgcgg actggatatc gaactggcgg caggcgatct
    22561 ggcaaagatc ctcgatgcca aatttgctga tatcgcccgc cgcgattaag cattgtgccg
    22621 gatgcaaaca tccggcactt tcggattact gccagctcac ctcacctttc ggttcataaa
    22681 cactcacatc cagcggcgag cttttctgga tatacgcttt cagcacttcg gcatcaataa
    22741 agccggtatt cacatagccc ggtttgttat caaggcgcgg atatccatca ccgccggtgg
    22801 cattgaagtt taatgtcgcc atacggtaag ttttcgccgg atcgaccggt tcgcctttga
    22861 ttttaaggtc gttcagtttg ccgtctttcg ccacaaagct aacgttggca aattgcgggt
    22921 aggcacctga atctggcttc atctgcgcga cggcggtcag gtaatcaatc acctctttac
    22981 cggtcatgtc ggcatacacc accacattgc cgaatggctg cactttcagc acgtttttat
    23041 agctgatatc gcctgcttcg atagaatcac gaattccgcc tccgctcatc accgcaaagt
    23101 cggcaccagt gcgatccatt tgggctgcca gaatcaaccg ccccatattg gtctgtacaa
    23161 aacgcacttt gtcacgatcg ccttccagac gaccattggt tttgcctatt ttcacttcca
    23221 gctgcgcttt gcctttgttc tggaacggtg ataacagcga gatcatttgc tggttttcag
    23281 cgatttcagg agtgtaaagc acgcgctcgc ttttcccgtc ttcccaggtc actttcttct
    23341 tcaggttcac cggaatcagc tggtagttaa ccattttcat ttcgccatta cgaaactcaa
    23401 aatcagcccg tcccacgtat ttgccccact catgcgcctg cacaatccag atgccgtttt
    23461 gttgatctgg tttgcatggc gtacccggca cgtaatcgac ctgttttttg ttttctgccg
    23521 ccatgcagac cggatcttgc gagtgaccac cgacgatcat cgccagcgat ccggcaggca
    23581 gcgcgcgtgc catctccaca tcgcccggtg cgttagagcc gtgctcacca ttatcgtaat
    23641 gccccatatg ggtcgccgcg ataataatgt ctggcttttc tgtctgttgc agctcctgaa
    23701 tcaccagctt cgcttcatcg gcgggcttac gaaattcgat atcagtgaag tattccaggt
    23761 taccaatttt tgctgtgtca tcggttgtca gcccaataac ggcaattttc agatcctgac
    23821 gcttaaacag cgcccacggt ttaaacaggc gctcgccagt acttttctgg tagatattcg
    23881 cggaaagcaa cgggaacttg gcccactttt cctgctggcg taatacggtg agcggattat
    23941 caaattcatg attaccgatc gccatcgcgt catagcccac cagattcata ccgcgaaaat
    24001 caggttcggc atcctgtaag tcagactcgg gcacgccagt gttaatgtcg ccaccggaaa
    24061 gtagcagcac gctaccgcct tcagccgcaa cctctttgcg gataccatcc accagcgttt
    24121 tttgcgccgc cagaccatat tcgccatatt cattgcgcca aaaatgccca tgatgatcat
    24181 tggtatgcag aactgtaatt ttgtaggttt tatcctgctc atacgccaga gcagtttcac
    24241 tcgccagtgt aaatgtggtt aacagcgcta acgccacgcc ccgctgcaat aatttcatac
    24301 ttctctccct gacctgattt caacgtgttg aaaaatagta tgacgcaaca tcatagacaa
    24361 gtctgcgtcc ggtgaagata aatctgcatc cagattagaa attctgtatt cggcgttgag
    24421 acttccttca gacgacgcat acaggtatgt tagtcgggta ataatcacaa ataagatcat
    24481 tctttacatc gcaaccctga aaaagtgact ttcctatggc aatgagtgaa caaacccagc
    24541 ctgtggcggg cgcggctgcg tcaacgacca aggcccgaac atcgtttggt attttaggtg
    24601 ctatcagcct ctcacatctg ctgaacgaca tgatccaatc gctgattctg gcgatttatc
    24661 cgctgcttca gtcagaattt tctctgacat ttatgcagat tggcatgata accctcacct
    24721 tccagctcgc ctcttcgcta ctgcaaccag tggtcggcta ctggaccgat aaatatccga
    24781 tgccgtggtc gttgccaatt ggcatgtgct ttaccttaag tggtctggtg ctgcttgcgc
    24841 tggcgggcag ttttggcgca gttctgctgg cagcggcgct ggtcggtacc ggttcatcgg
    24901 tctttcatcc ggaatcttct cgcgtagccc gtatggcttc cggcgggcgg catggcctgg
    24961 cgcaatctat ctttcaggtc ggcggcaact ttggcagttc cctgggaccc ttgctggcgg
    25021 cggtgattat cgcgccttat ggcaaaggca acgttgcctg gtttgtgctt gcggcactgc
    25081 tggcgatcgt ggtgttggcg caaattagcc gttggtactc ggcacagcac cgaatgaata
    25141 aaggaaaacc caaagcgacg ataatcaatc cactgccgcg caacaaagtg gtactggcag
    25201 tcagcattct gttaatcctc attttctcga aatatttcta tatggcgagc atcagcagct
    25261 attacacctt ttatctgatg caaaaattcg gattatctat ccagaatgcc cagcttcatc
    25321 tgtttgcctt cctgtttgcc gttgcggcag gtacggtgat cggcgggcct gtaggggata
    25381 aaattggacg gaaatatgtg atttggggct ctatcctcgg cgttgcgccg tttacgctga
    25441 ttttacccta cgcccgcctg cactggacgg gggttttaac ggtgattatt ggatttatcc
    25501 tcgcttcggc attctctgcc attctggtct acgctcagga gctacttccg ggacgtatcg
    25561 gtatggtttc tggactcttt ttcggttttg ctttcggcat gggaggtctg ggagcggcag
    25621 ttctggggct tatcgccgat cacaccagca tcgagttagt ctataaaatc tgtgctttcc
    25681 tgccactatt ggggatgttg accatattcc tgcctgataa ccggcataaa gactgatttc
    25741 attgcagcca aaggcaaact ttggctgcat cgttttacag tcgccataag cctttcctct
    25801 gttaaaccgc cttctgtttt ccgaaccgtg ctttttttgc agctaattcc atttttctgc
    25861 aaaattcaac aattattcat aaacatcatc aatgaaattg tcataaacta ttacctgtaa
    25921 ttttggtttt cccagccaaa aatggacaat aaccaccagg aaaaggagac ggaatgcatc
    25981 acgccacccc gcttatcacc accattgttg gcggccttgt gctcgccttt atcctcggca
    26041 tgctggccaa taaactacgt atttctcctc tggtgggata tctgttagcg ggtgtgctgg
    26101 caggaccatt cactccgggc tttgttgccg ataccaagct tgcgccggaa ctggctgaac
    26161 tgggcgttat tttgctgatg ttcggcgtcg gtttgcattt ttcgctcaag gatttgatgg
    26221 cggtaaagtc catcgccatt cccggcgcga tcgcccagat agccgtggcg acgctgctgg
    26281 gtatggcgct ctccgccgtg ctgggctggt cgttaatgac cggtatcgtg ttcggcttat
    26341 gtctttccac cgccagtacc gtggtgttac tgcgcgcact tgaagaacgg caattaatag
    26401 acagtcagcg tgggcaaatc gccatcggtt ggttgattgt ggaagacctg gtgatggttc
    26461 tgacgctggt gttgctgccc gcagtggcag gaatgatgga acagggcgat gtgggctttg
    26521 ccactcttgc agtcgatatg gggatcacca tcggcaaggt gatcgcattt atcgccatta
    26581 tgatgctggt aggtcgccgt ctggtgccgt ggattatggc acgcagcgcg gcaaccggtt
    26641 ctcgcgagct gtttaccctg tcagtgctgg cgctggcgtt agggattgcc tttggtgcgg
    26701 tagagctgtt tgatgtctcc tttgcactcg gtgcgttctt tgccgggatg gtactgaacg
    26761 agtctgaatt gagtcaccgt gccgcccacg atacgttgcc attgcgcgac gcgtttgcgg
    26821 tgctgttttt tgtctcagtc gggatgttgt ttgatccgtt aattctgatt cagcaaccgc
    26881 tggcagtgct ggcgacgctg gcaattattc tgtttggtaa atcggtcgcg gccctgttcc
    26941 ttgtccgtct gtttggtcac tcccaacgta cggcattaac catcgccgcc agcctggcgc
    27001 agattggtga gttcgcgttt atcctggcgg gactgggaat ggcattgaat ttactgccgc
    27061 aggccggaca aaacctggta ctggcagggg cgatcctgtc gattatgctc aacccggtac
    27121 tgttcgcact actggagaaa tatctggcga agaccgaaac gctggaagag cagacgctgg
    27181 aagaggcaat cgaagaagag aaacagatcc cagtggatat ttgcaaccat gcgctattgg
    27241 tgggttacgg tcgtgtaggc agcctgctgg gggagaaatt gctcgcctct gatattccgc
    27301 tggtggtgat tgagacgtca cgaacccgtg ttgatgagct gcgagagcgt ggggtccgcg
    27361 ctgtattggg caatgcagcg aacgaagaaa ttatgcaact ggcacatctg gaatgtgcaa
    27421 aatggctgat cctgacgatc cccaacggtt atgaagcggg tgagattgtg gcgtctgccc
    27481 gcgcgaaaaa cccggatatt gagattatcg cccgcgccca ttatgacgat gaagtgacgt
    27541 atatcaccga acgtggcgcg aatcaggtag tgatgggtga acgtgaaatt gcccgtacta
    27601 tgctggaact gctggaaacg ccaccggcgg gtgaagtggt gacggggtaa acggtggttg
    27661 ccggatgtaa aatatgaaaa atatgtaggc atgataagac gcgccagcgt cgcatcaggc
    27721 atcgagagcc aaatgccaga tgcggcgtga acgccttatc tggcctacga accgacgata
    27781 acttaacgat cccagtaaga ctcttcctgg ctgtcttcac gctccggcaa gccgcgcgtt
    27841 aaacgaggtg aatgctggtt cagtacctga tagctcacac ggttagcata tttacacacc
    27901 tgcgctaacg atgaataagt taaccaggtg aatttatgtt tgctggagtt tggtacgttg
    27961 ctacgatggt agctgttggc ggtaatgtca tgcagcaacg ctgccaatgc gccatccccc
    28021 gctccattag tgttcatgat tttttccggc ccgcccatgt acggcgcaat gtgcgaatag
    28081 acacgcagcg gattctggca atccttgtgg cgcatggcgc ggctaaactc atactggttg
    28141 aattccgcta tagcgcccgg cagcagcgga tgctgggttt tacgtttcgc ttcgtcttcg
    28201 gtaaagcccg ccatatacaa gccgattggc ccggcggtgc acagcaccag ctctacccag
    28261 tccagcgcct tgtcagatgc caacaacgga tcgctttctc cggtcaacgc ttcggcttca
    28321 tcttcgttca tcgcaaggat agaaacgtgg tctttgagga attgctgcca ccactgcgga
    28381 ttctcggcaa tgacaaactt ggtgcccagc gtcagcacca ccggtacgtt atatttcttc
    28441 gcgtactcaa tggctttcat ggttgcttcc ggcatgggtt cacccggctt gcaacgcacc
    28501 agatatgagg tgagaaccag tgccgaggct ccggcaatca catcttccgg aatgctttca
    28561 gcccgcagct ggttcatgtg gcctggactg atagcaaagg tacgttcccc ggactcgcca
    28621 atcagcgtaa agcaacgacc aatcggacca tccacgcctt gtagatagtt aagatcggta
    28681 cggctggaag tgttacacag gtaacgatag gcataactgc caatttcaat attgctgcac
    28741 atgacgccca gcagcaccga acggtcgtcc gcgagcaccg agtagttgtg catggtgtta
    28801 ccaatggtgc cacccgcaaa ctgatgggta atcaggtttt tctgttttag ctcctgataa
    28861 agcacttcgg ctacgtcatc ctcaatcacc agtgaatgcc cggcgcttaa tccataacgc
    28921 tcaatgaatt catcatccac tttcgcttca atatcgacca gcgtctggtc aatacctacg
    28981 acccaggcgg cgctggtttc gttttctggc tggaattgct gaagcagcgg atcgcgtgca
    29041 tttacaggga agtaatgttt ggatttacgt ttaccgggaa atttcatggt tgtttttacg
    29101 ggtggttaac tgagcggcga atgttaacac aatctgacgg gagcgcgcga gacgcagcca
    29161 gtttcaatat cagcaggcgg gatcgcattt tcatggcagt ggttccttac aatgacgtaa
    29221 tttgaaagga gtttttgtat gaagccggaa aacaaactac ctgttctgga ccttatttct
    29281 gctgaaatga agaccgttgt gaatactctt cagccggatt taccgtcctg gcccgcaacg
    29341 ggaacgattg ctgagcaacg acagtattac acgcttgagc gccgattctg gaatgcgggc
    29401 gctccagaaa tggcaaccag agcttacatg gttccaacaa aatatgggca ggtggaaaca
    29461 cgtctctttt gtccgcagcc agatagcccg gcgacgctat tttatttgca tggaggcggt
    29521 tttattctcg gcaatctcga tacccacgat cgcatcatgc gcctgctggc aagctacagc
    29581 caatgtacgg tgattggtat taattacacc ctttcacctg aagcgcgttt tccacaagcg
    29641 atagaggaaa ttgtggctgc ttgttgttat ttccaccagc aggcggagga ttatcaaatc
    29701 aatatgtccc gcattggctt tgccggtgat tccgcaggtg ccatgctggc gctcgccagt
    29761 gcgttgtggt tgcgtgataa acagatcgat tgcggtaaaa ttgcgggtgt tttgctgtgg
    29821 tatgggcttt acggattacg ggattccgtg actcgtcgtc tgttgggcgg tgtctgggat
    29881 ggcttaacgc aacaggattt gcagatgtat gaagaggcgt atttaagcaa cgacgcggac
    29941 cgcgagtcac cgtattactg tctgtttaat aatgatctca ctcgcgaagt tccgccctgt
    30001 tttattgccg gggcggagtt cgatccgctg ctggatgaca gccgtctgct ttactagacg
    30061 ttagcggcgc atcagcagcc ctgtgagttc aaactctacc caggtacgct gcatgccttt
    30121 ttgcattatt cacggatgat gaaaaccgcc gacgaggctc ttcgcgacgg cgctcagttc
    30181 tttaccgctc agctttagcg atacgcggca acaagattag ccatcatttc gatatgttcc
    30241 ggcgtggcat taagcgctgg aatatattca tattttttcc cgcccgcacc gaggaagacc
    30301 tcacggtttt gctcggcaat ctcttccagc gtctccagac aatccgcagc aaagcccggg
    30361 cacatcacct gtatatgacc tacgcctttt tctccgagca ttttcagcgt ttcgtcggta
    30421 taaggcatca gccagggttc ccggccaaag cgcgactgaa aggtcatcat cactttttcc
    30481 ggtaccatcc ccagtgcgga agccagttcg cgagtcgttg tgcggcaacg ttgcgggtaa
    30541 tcatcgcctt catctgcata acgctgggga atgccatgat aagagagcag cagcagatcc
    30601 ggttcgccat gtttggcaaa agaagcgcgt acgctgttcg ccagtgcatt aatgtaatcg
    30661 tggttatcgg cgtaatcacg aataaacgat atccccggaa tgctacgttt gcgcgccaga
    30721 atgcgtgcca gttcatccca taccgcaccg accgtagaac aggagaattg cggataaagc
    30781 ggcagcacca caatatgatc tacatgctct gccaggagtt catcgacgga gctttccagt
    30841 gatggcgagc cgtagctcat gcccagcgcc actggcgtat ccggtaaacg ttgtgccaac
    30901 gcctgctgtt gctggcggct gtaaaccatc agcggcgagc cgtcttccat ccagacagag
    30961 gcgtacagct tcgccacacg cggcgagcgc agcggcaaaa tcacgccgcg cagcaacggc
    31021 caccataaca accgtgaggt atcaaccacg cgtctgtcgc ttaaaaattg tttcagatag
    31081 cgttttaccg cttcaggtgt gggggcatcg ggcgtaccca ggtttgccag caggataccg
    31141 gttttagtct gacgcattac cgcctcttat cgattcaact tgttgataat tgtagcggaa
    31201 aagtgaggaa gaagaactaa ttgctgtgag aggtattgcc tgaggtggat aagcagacct
    31261 gcccgaaagc agatctgctg cggcttgatt agccgaggat tttttccaga tcagcgcgaa
    31321 cttcagcaac ctgcttggta ccgtcaactt tcgcgtattt ggtgttaccc gcttccgctt
    31381 ctttggagta gtagccgatc agcggtgcag tcatctgatg gtattcaacc agacgtttac
    31441 gcacggtttc ttcctgatcg tctttacggg tagtcagttc ttcaccggta acgtcgtctt
    31501 tgccttctac tttcggcgga ttgaatttaa cgtgataaac acgaccagac ggcgcatgaa
    31561 cgcggcggcc tacgatacga tcaacaatca gttcgtccgg tacgtcgaat tccagaacgt
    31621 aatcaacatt gatgcccgct tctttcatcg cgtctgcctg cggaatggta cgcgggaagc
    31681 cgtccaacag gaaaccatta cggcagtctt cctgagcaat gcgctcttta accagcgcga
    31741 tcaccagttc gtcggtgacc agtttgccag catccataat gtcttttgct tgtttaccca
    31801 gctcggagcc agatttgacc gcagcacgca gcatatcgcc agtggagatt tgcggaatac
    31861 catatttctc catgatgaac tgagcctgag tccctttccc cgcgcccgga gcgccaagca
    31921 gaatgatacg cattgcgaaa atccccttaa aatgtgtcga tttttttgaa aaagcgataa
    31981 acgataccac caacaggcga ttgcctcaag aaaggcacct gaggctgaaa cggttaatgg
    32041 cgagataatt tgcggaaagt acagatgatg aaaagaaaaa tgccggatga cacaaaggtc
    32101 atccggcatg acatcaggaa accagcagct gattcatacg acgaataaac aggttcggat
    32161 cttccagcgt gccgcgttct gccagcagcg cctgatccag cagcagttct acccactcgc
    32221 tgaacttggc ttcatcttca gtatctgccg cacgtttcac cagtacgtga tccgggttca
    32281 gttcgaagat gtatttcact tctggcactt tctggcccgc cgcagcgaac agtttcgcca
    32341 tctgagtgct catttcgtcc gcgtcggtcg aaacgatcgc tggcgtatcg gtcagacggt
    32401 gagtcagacg gacatctttc acgcgctcgc cgagcagagc tttcacgcgg tcgatgaacg
    32461 gagtcagtgc tttctccgct tctttcgcgc tctcatcaac ttcgtcagcc agtttttcca
    32521 gcgactcgtc aactttagac accgactgga acggtttacc gtcgaactca gtcagatagt
    32581 tcatcatcca ctcatcgatg cggtcggaaa gcagcagaac ttcgatgcct ttcttacgca
    32641 gcagttccag gtgcgggctg ctcttcgctg ccgcatagct gtctgcagtg atgtagtaga
    32701 ttttctcctg cccttctttc atgcgggaaa cgtagtcttc cagagacacg gtctgcgcag
    32761 aagaatcggt ataagtagaa gcaaaacgca gcagtttggc gatcgcttcc tggttagcga
    32821 aatcttccgc cggaccttct ttcagtacca ggccaaactg ttgccagaag gtctggtatt
    32881 tttccgcgtc gtctttcgcc agtttttcca gcatttgcag cacacgcttg gtcagcgcat
    32941 tgcgcaggtt acgcgttacc gtgctgtcct ggaggatttc acgggaaacg ttcagcggca
    33001 gatcgctgga gtcaatcaga ccacgcacga agcgcagata gttcggcatg aactgttcag
    33061 cgtcgtccat gataaacacg cgttgaacgt acagtttcag gccgtgttta tgatcacggt
    33121 tccacatatc ccacggagcc tgggacggga tgtacagcag gctggtgtac tcctgcttac
    33181 cttcaacgcg gttgtggctc caggtcagcg gatcgttaaa gtcgtgggcg atgtgtttgt
    33241 agaactcttt atactcttca tcggtgattt ccgacttgtt acgagtccac agcgcctgcg
    33301 ctttgttgat ttttgcccag gagataacgg tttcgccatc tttctcttcg cgtttttcga
    33361 tctctaccgg cagcgcgata tggtcggagt atttgctgat gatggaacgc acgcgccagt
    33421 catcgaggaa ctcgtcttcg ccttcacgca gatgcagggt gatttcagta ccacgatctt
    33481 ctttggtgat atcggcaacg gtatattcac cttcgccagc cgattcccag aagacgccgt
    33541 tttctggttt ttcgcctgcc gcgcgagtac gcacggtcac tttgtcggcc acgataaacg
    33601 cagagtagaa accaacacca aactgaccga tcagctggct gtctttcgct tggtcagaac
    33661 ccagggattc gaggaatgat ttggtaccgg atttagcgat agtccccaga tggtcaatca
    33721 cttcgtcgcg ggtcatcccc acgccgttat cggagatggt cagcgtgcgc ttgtctttat
    33781 cgaaagagac acgaacgcgc agttcgccat caccttcgta caggtccggg ttagatagcg
    33841 cacggaaacg cagcttgtcc gccgcatcgg aggcgttaga gataagctca cgcaggaaga
    33901 tttctttatt ggaatagaga gaatggatca tcaggtgcag aagctgtttc acttctgact
    33961 gaaaaccacg agtttcttgt cctttcatgt aggtctacct caataatgcc attttaaggt
    34021 taaaaacagg atgagggaga gatggggaca agggagaata atttcaagcg agagcaggtg
    34081 ctcctgctct cgtttgctta aaaacgaatc ttatgacgcc cggcgaggga gtgtgacagc
    34141 gtggtgccat cgaccatttc cagttcgccg ccaaccggta cgccatgagc gattcggctg
    34201 gcttccacgt catattgcgc gcaaagctcg gcaatgtagt tagcggtagc ttcaccttca
    34261 accgtgggat tggtggcgag gatcacttca gtgatttttt cctctgccag acgctgttcc
    34321 agacgatcaa gcccgatatc atccggaccg atgccgtcca gcggtgacag atgccccatc
    34381 aacacaaaat aacgaccaga aaactgcccc gtctgctcaa tggcgtagat gtccgccgga
    34441 ctctccacca cacagatttg accgttttcc tgacgacgcg gattcgaaca gatgttacag
    34501 acttcctgtt cggtgaaagt gcggcaatcg gcgcagtggc cgatttccga catcgcccgg
    34561 gtgagcgcct gcgccagacg catcccgccg ctacgatcgc gctgaagcag cgtgaacgcc
    34621 atacgctgcg ccgacttcgg gccaacgccc ggcagacagc gcagtgcttc cataagctgt
    34681 gttaacagcg ggctggtttg catcagaacg gcatcttaaa gccaggcggc agctgcattc
    34741 cggaggatac agaggccatt ttttctttct gcgtttcttc aatacgacgt gctgcgtcgt
    34801 tgaatgctgc agccaccagg tcttccagca tctctttgtc gtcttccagc aggctcgggt
    34861 cgatctctac gcgacggcag ttgtgtgcac cgttgatggt cacttttacc agacctgcgc
    34921 cagattcgcc ggtaacttcc agctgcgcga tctcttcctg cattttctgc attttttctt
    34981 gcatctgctg ggcttgcttc atcaggttac ccagaccgcc tttaccaaac ataggtttct
    35041 ctctcaatca cgttaaggat gacgaacgta agctgtgctt acgatcaaat ggggcggata
    35101 ctttcttcat ccagctccgc atcgaaaaac cgacgcaggg tctgaatatt attatccgca
    35161 ataatggact cgcgcgcctg cgcaagtttt tcttcgtata tcgcctgacg ccactccagc
    35221 ggcgtacgca ccgcgggatt atcatcttca acgatagtca gttcaaccgt tgaacctttt
    35281 aacatgctca acgcttcagc cagtttttgc tgtgcaccgc ggttgttcaa atgccgctga
    35341 gaggagcgca aatgcagaca tactgcgttg tcgctctcct ctttccaggc atttaacgcc
    35401 acctgttcga ccagttttgg tagcgaaagt tggctcacct gtgccgccca cgggtcgcgc
    35461 tcaatggctt ctgccgctag cttcaccgcc agttccggcg ttttttcatg ttccagcgct
    35521 tttttcagcg ccttcggcgt ggcgaccact tctttttgct gcatcaccgg agtggtcgcc
    35581 ttccagcgat acgcttcttt tttggctggc gctttttcca gcgccgatgg caccggacgc
    35641 gcctgaacgc gatcggtgac cgaagccagt ctttccagcg cagcgttatt caccggccgc
    35701 gcgcgggtag cggctgccgg ttcactcttt tttgctttgg ttgctccctg cacgcgctgc
    35761 aactgctggc gcgccgccag cacctggctg gtggtttccg ggagcggtac agtcggtgcc
    35821 tgctgcggcg ctgattgcgg cggcaccagg gttggcgtca ttactgccgt tggcgcgacg
    35881 ggtgcaaagg actggcgtgg cacttctggc tcaggcagcg gcatacgcgg atggaacgcc
    35941 agcgcgcgca gcagcgtcat ctcaacgccc atgcggcggt ccggcgcata cggtaattct
    36001 ttgcgaccaa tcaacagcgt ctgatagtaa agctgaatat ccgtcggcgg tatggtgcgc
    36061 gccagttcac gcatccgcag ctcgatggcg gccatgtcgt tgccaagtgc agcaggcgaa
    36121 agttgtacca tcgcaatacg atgcaacaag ccgagcattt ccaccagcaa cgcttcccac
    36181 tcgataccac gggcagcggc ttcattaatc agtgccatta cgcgctcgcc gttgccctcg
    36241 accatcgctt caaccagcga cagcgcctga tcgtcgtcaa gcgtacccag catcgcactg
    36301 accgcctggg ttgaaacctg gccgtcaccg ctggcaatcg cctggtcggt cagacttaag
    36361 gcatctcgca ggctgccttc agcggcgcgc gccaacaatt gcagcgcccg cggctcgtga
    36421 gcgatatgtt cttcattgag gatgtgctca agctgatggc gaatttgctc gacatccagc
    36481 gccttgagat gaaattgcag acagcgtgac aaaatcgtca ccggcaattt ctgtggatcg
    36541 gtcgtcgcca gcagaaactt aacgtgctcc ggcggctctt caagggtttt taacagtgcg
    36601 ttaaagctgt ggcgcgacag catatgcact tcgtcgatca gataaacttt gaaacgacca
    36661 cgcgccggag cgtactggac gttatccagc aggtcgcggg tatcttcaac tttggtgcgc
    36721 gaggcggcgt cgatttcaat cagatcgaca aagcgcccct gctcgatttc acgacagtta
    36781 tcgcacacgc cgcacggcgt cgcggtaatg ccggtttcgc agtttagccc cttcgccagc
    36841 agtcgggcga tagaggtttt tccgacgcca cgggtgccgg aaaaaagata agcatgatga
    36901 atacgcccta acgacaagcc gttcgccagt gcggtcagca catgttcctg gccgacgacg
    36961 tcagcaaagg tttgtgggcg ccattttcgg gctaaaacct gataactcat tggcaggctc
    37021 tgaaacgctg gaaggtggat tcacgaaggg gtaatgctaa cacagccccg tcagaacggc
    37081 gaggctgaat gcacatcttg tgctgtccgt agcgtgggca gcacaagact ggcgataatt
    37141 aatggcccgg gaacgggaca aggctgtagc tggtaatgcc ctgtttttcg agacgctgtt
    37201 cgccgccgag atcaaacagg ttgataatga acgcagcgtc agccacttca ccacccagac
    37261 gacggatcag tttaacggtc gcttcgatag tgccgccggt tgccagcaga tcgtccacca
    37321 ccagaacttt gtcgcccggt ttgatggcat caacgtggat ttccagctga tcggtgccgt
    37381 attccaggtc gtaattttca ctgatggttt cacgcggcag tttgcccggt ttacggaccg
    37441 gtacaaagcc aacgcccaga cccagagcta ccggagcgcc aaacaagaag ccacgcgctt
    37501 cggtgccgac aactttggta atgcccgcat ttttgtaacg ctcaaccagc aagtcgatgc
    37561 tgagagcgta agctttcggg tcttccagta agctggtgac atcgcggaaa agaatgccgg
    37621 gttttgggta gtcctgaatg cttttgatgc tatttttgag atactcaagc tgctgtgcag
    37681 tcgcggtcat aagtgtatgc ctgcttgtta cggtggtact cacggcgcgt tttaaacgta
    37741 tcaaaagtaa ctgttgttta acctttgacc gcctgcgcct gtgctcgaaa acgccagaat
    37801 gtactggctg tacgcaataa ttgcaactgc gattgttgtg cttcagtgct tttcttgctt
    37861 ttcatcaatc accggaattc gccacatata gaaaagcaaa caggcgagaa ttaccagcaa
    37921 catgatgcgc acccatggca tctggacgaa ccacagagaa atggcaaacg tgagcaaaat
    37981 aagcaaaatc gcccgcggtt tgacgccgcg cggcatcgca tgatgtttct gccagaaacg
    38041 tagatagctg ccaaaccatg agcggtacag caaccaggcg tgaaagcgcg gggaagaacg
    38101 ggcaaagcac caggccgcca gcaggataaa cggcgtcgtc ggtaataccg gtaataccac
    38161 gcccagcgta cccagcacta ccgccagcca gccaatgatg attaaaatga ttcgttgcat
    38221 attgagtgtt gtcagcttac atagtggcta ctttagcata acaattatca ttttcattga
    38281 ggtcttatcg tgaaaaccgc cctgctgctg gaaaaactgg aaggacagct cgctacgctg
    38341 cgtcagcgtt gtgccccggt gtcacagttc gccacgctaa gtgctcgttt cgacaggcat
    38401 ctttttcaga ctcgtgcgac aacactacag tcttgtctcg acgaggcggg cgataatctg
    38461 gctgcgcttc gtcatgcagt tgagcagcaa cagctgccgc aagtggcctg gctggcggaa
    38521 catctggcgg cacaactgga agccatcgcg cgtgaagcct ccgcctggtc attgcgcgag
    38581 tgggacagtg cgccaccgaa aatttcccgc tggcagcgta aacgtattca gcatcaggat
    38641 tttgagcggc ggctacgtga gatggttgcc gaacgcagag cccgcctggc gcgggtgacc
    38701 gatctcgtgg aacagcaaac gctgcatcgt gaagtggaag cctatgaagc gcgcctggca
    38761 cgctgccgcc atgcgctgga aaaaatcgaa aacaggctag cgcgcttaac ccgctagcaa
    38821 tggaaagagt atgtcacttg aaaatgcccc tgacgatgtc aaactggctg tcgatttgat
    38881 tgtgctgctg gaggaaaatc agatccctgc cagtaccgtg ctgcgcgcgc tggatattgt
    38941 aaagcgtgat tatgaaaaga aattaacgca cgatgatgag gcggaaaagt gagaaataaa
    39001 tggggccgct aaatgtcccc taagattgcg ggcaaacgca aaactgcctg atgcgctacg
    39061 cttatcaggc ctacaccgct cctgcaatat attgaattct tgcggtttcg taggccggat
    39121 aaggcggtca cgccgcatcc ggcatgaaca acgcgcactt tgtcagtaat ctcggggcaa
    39181 ctaaatgtcg ccccctttgg ttattaccca accgctggtg tcggatcgtc gcctttgtag
    39241 tcgcgtttta cttccgtcac ctcatcgccc ttctcgttgt gcagatgcac ttcaagctgg
    39301 ttaaaggcaa tgttgatgtc gttttcacgg cacagctgat cgatagtacg gttcagctca
    39361 tcgacagtac ggctacggtc acgcagttca cgcacataca gacgcagctc atgatccaac
    39421 gtgctggcac caaatgccgt aaagaagact tccggcattg gttcgtgcat cacccttggg
    39481 tgctcagtcg ccgccttcag taacacttta cgcacttttt ccagatcgga gccataggcc
    39541 acgccgagac ggatcaccag acgcgtagta gtgtcagtca acgaccagtt gatcagacgc
    39601 tcggtaacaa acgctttgtt cgggatgatc acttctttgc gatcgaaatc ggtaatcgtt
    39661 gtcgcacgaa tacggatctt acttaccgtc cccgagaagc taccaatggt taccgtatcg
    39721 ccaatacgca ccggacgttc gaacagaatg atcaaaccgg agacgaagtt accgaaaatt
    39781 tcttgtaaac caaaaccaag acctacggat aatgctgcgg ccagccactg gagtttatcc
    39841 caagagacgc ccagcgatcc gaacaccgtc atcgcaccaa cggcaataat gatgtagtta
    39901 aggatggtag taatggcata cgacgcgccc tggcgcatat tcagtcgcga gagcaccagc
    39961 acttccagta aaccaggcag gttgcgaatc aacgcccagg ccaccattga ggcgataatc
    40021 gcaaacaaca gactgcccat ggtgacgttt ttcaccaccg cagcgccagc ttcagtgccg
    40081 ttgtaatgcc agagcgtgat gctgtcgaga tagctgaaca cggtgatcaa atcggaccaa
    40141 attgcccaga acatgacacc gaacagcgca aacatcagca acatggtaat acgcagcgtc
    40201 tgctggttaa cttgctccag tgcaatggtg ggttcttccg gcggttcggc accttctgcg
    40261 ccctctttca ccagattctg ccgacgcgcc agcgcacgac gccaggcgat acgccgcgcc
    40321 gctacgctta agccgcgcaa taccgtctgg tagagcaagt tccagatgat caccagataa
    40381 acggtttcaa tccaacgccc ggagaggcgc agcgtggtgt agaaataacc cgtggcagtc
    40441 agcaccatca atgcaatcgg gataatcgac agcacggtaa tggtgactag tcgcatggtg
    40501 tgcgactctt tatcacgcca gctttcgcgg cacattggcc ataccaggaa ggcaatcagc
    40561 agcaggttga agaaaatcat cgcctgcccc agcacatcat ccatcagatg cagcggggaa
    40621 agttctgcca ccacagacca gaaatggata ggcagcaacg cgagactgat gcggacaatt
    40681 tgccgacgcc agtggctggt ctgctgctcc ggcatgccga agtgacgcac ggcaacgcca
    40741 tttttctcca gtaccttcca gcacaggcca aacaccagcc agaaaatcgc cagtttttta
    40801 ctgaacgacc atagcagttc gctgatgttg agctgcatgg tcaacagaat caggccaacc
    40861 gcgagaataa tcaggcacac cggcagcgcg cggatcaggt cgataaggat cgcttttggt
    40921 gtgttgagct ggctatcgtt acgcagggaa cccacagcgg aagccagttt ctgttgatac
    40981 gctttcagcc agcccagacg ccagtggatc agcccggcaa tcaacagcag cggcaaaccg
    41041 gcgaggaaag cgataaaaac ggcaggccag gctttttccc agttcaccgt gattttcatc
    41101 gacttaaatt catctttcag gctttgcggg aacgctttga tccagtccca gtccattgga
    41161 cggttactgt tcacccaaaa gatttgctga gtcaggatgg atttcaggtt tttcgatacg
    41221 ctcattaact gctgctggtt gatttgcagg ttaatggcca tcatcagctg gttacccaac
    41281 tgtttgttga gttgatccag caattcgcga cgcatatcaa ccacttgcaa taacgcatcg
    41341 tgaacttcgc tgttgacttc gttggtgtga ccttcttcca gtttgttgac gaacgcatcg
    41401 ctctggaaga gtgcatcacg ctgctggtta acttcaaact gttcgagacg caaatccgcg
    41461 atgcggttgg tcatgttttc cagttcatcc gccgagggca gcgtttgttg ttgctggtaa
    41521 aggatacgag acaacagcag gctgcccttc aggacggcaa tctgctcttt aatattgcgt
    41581 tccgattgca gcgcccgctc cagccagttt ttgactttaa tgttttgctg catcaactga
    41641 ttaccgtttt cagtcgcggt aatcagacgc tgacttaact gctggttaat ttccagttcc
    41701 tgtttcacca gcggattagc ctgaatacgc gcggcttcat ccggggagac ggcttcctgc
    41761 gccgtttttt cagttaaagt caggcgcttg ctgtttaccg cttcttgcaa cagctgcaac
    41821 tggtgctcca gacgggcgct gttcgccgtc acgtaatcac gttgcttttg caaggtatcc
    41881 tgcaagacgg tgttcccttc caggctttta cgctgctggt caatctccgc attcagcaac
    41941 gcctgctgag cctgcattaa cactttctgg ctgggacgta aggcagtctc gccgacatca
    42001 gtcccatcca gacgactgcg aatttgttgc agctgctgcg aagcgttata catcgcattt
    42061 tgcacgcgtt cgggctgcgt ctgtaacgaa accagctggc tgttataaga cgccagatcg
    42121 ttttgtgcgt tttgcaaatc gtccagcgcc tgggcaacgc gagtttccag ctggcgcaac
    42181 gaaagcgtac tcagaatttt gcgcgtttct tcgtcgttat cgacatcgct aagtgctgtt
    42241 aacgccgcgg tcgcctggcg cattttttcc ggcgcttcag cgactttttg ccgtagctga
    42301 actgtctctt cttttatgcg atcgatttta tcgagggtgg ctaatgtatc tgtcagatcc
    42361 tgctgcacca gtttgtcctg agcagaaaga tctttttgtt tatttagtga gtcaagttgc
    42421 gcctgcaggt ccgcttttgt cggcagatca ccattcgatg acgcccgcgc aaacgccgtg
    42481 ttctggcata acaagacaaa aacaaaagca ataaatgctg aaaaaacaaa atgccgtgat
    42541 cgtttgtaat actggaacat agtcatgatg aatgaaggtt tctgaacctg aagaacgacc
    42601 tgaaaaagtc aaaccgcaag aatatcacga cgcagtgaac cagaatagca acgacgaaaa
    42661 tgtccaggaa aaatcctgga gtcagattca gggttattcg ttagtggcag gattacgaag
    42721 cgtggggcac aggagataca tctccagtaa gatggcgacg taatcgcggg cttctttttt
    42781 aagatcaaaa gattgcgggg caaagagcca gttttccatc aggccggaaa tatagccgcg
    42841 cataataatt gctgcccgac gcgtcattaa atccgcaggc aacattttcg cttcaataca
    42901 atgttttaac gtttgttcta tacggtcata actttccaga cagagattac gttgtgcctg
    42961 ttgcacaaca gccatttctc cgacaaattc gcatttgtgg aatataatct ccatcaataa
    43021 tcgacgtcgt tcttctgtca ccgtggattc aagaacatga attaatatct ctcttaatac
    43081 tgagagtgga tcgccaggga attttgcctg atactcaagc tctagttcac caatattgga
    43141 ttctgacagt tcccagatct cactgaacaa atccgacttg tctttaaaat gccagtagat
    43201 tgcaccgcgc gtaacgccag ctgcttttgc aatctcgccc agcgaggtgg atgatacccc
    43261 ctgctgtgag aaaagacgta gagccacatc gaggatgtgt tggcgcgttt cttgcgcttc
    43321 ttgtttggtt tttcgtgcca tatgttcgtg aatttacagg cgttagattt acatacattt
    43381 gtgaatgtat gtaccatagc acgacgataa tataaacgca gcaatgggtt tattaacttt
    43441 tgaccattga ccaatttgaa atcggacact cgaggtttac atatgaacaa aaacagaggg
    43501 tttacgcctc tggcggtcgt tctgatgctc tcaggcagct tagccctaac aggatgtgac
    43561 gacaaacagg cccaacaagg tggccagcag atgcccgccg ttggcgtagt aacagtcaaa
    43621 actgaacctc tgcagatcac aaccgagctt ccaggtcgca ccagtgccta ccggatcgca
    43681 gaagttcgtc ctcaagttag cgggattatc ctgaagcgta atttcaaaga aggtagcgac
    43741 atcgaagctg gtgtctctct ctatcagatt gatcctgcga cctatcaggc gacatacgac
    43801 agtgcgaaag gtgatctggc gaaagcccag gctgcagcca atatcgcgca attgacggtg
    43861 aatcgttatc agaaactgct cggtactcag tacatcagta agcaagggta cgatcaggct
    43921 ctggctgatg cgcaacaggc gaatgctgcg gtaactgcgg cgaaagctgc cgttgaaact
    43981 gcgcggatca atctggctta caccaaagtc acctctccga ttagcggtcg cattggtaag
    44041 tcgaacgtga cggaaggcgc attggtacag aacggtcagg cgactgtgct ggcaaccgtg
    44101 cagcaacttg atccgatcta cgttgatgtg acccagtcca gcaacgactt ccttcgcctg
    44161 aaacaggaac tggcgaatgg cacgctgaaa caagagaacg gcaaagccaa agtgtcgctg
    44221 atcaccagtg acggcattaa gttcccgcag gacggtacgc tggaattctc tgacgttacc
    44281 gttgatcaga ccactgggtc tatcacccta cgcgctatct tcccgaaccc ggatcacact
    44341 ctgctgccgg gtatgttcgt gcgcgcacgt ctggaagaag ggcttaatcc aaacgctatt
    44401 ttagtcccgc aacagggcgt aacccgtacg ccgcgtggtg atgccaccgt actggtagtt
    44461 ggcgcggatg acaaagtgga aacccgtccg atcgttgcaa gccaggctat tggcgataag
    44521 tggctggtga cagaaggtct gaaagcaggc gatcgcgtag taataagtgg gctgcagaaa
    44581 gtgcgtcctg gtgtccaggt aaaagcacaa gaagttaccg ctgataataa ccagcaagcc
    44641 gcaagcggtg ctcagcctga acagtccaag tcttaactta aacaggagcc gttaagacat
    44701 gcctaatttc tttatcgatc gcccgatttt tgcgtgggtg atcgccatta tcatcatgtt
    44761 ggcagggggg ctggcgatcc tcaaactgcc ggtggcgcaa tatcctacga ttgcaccgcc
    44821 ggcagtaacg atctccgcct cctaccccgg cgctgatgcg aaaacagtgc aggacacggt
    44881 gacacaggtt atcgaacaga atatgaacgg tatcgataac ctgatgtaca tgtcctctaa
    44941 cagtgactcc acgggtaccg tgcagatcac cctgaccttt gagtctggta ctgatgcgga
    45001 tatcgcgcag gttcaggtgc agaacaaact gcagctggcg atgccgttgc tgccgcaaga
    45061 agttcagcag caaggggtga gcgttgagaa atcatccagc agcttcctga tggttgtcgg
    45121 cgttatcaac accgatggca ccatgacgca ggaggatatc tctgactacg tagcggcgaa
    45181 tatgaaagat gccatcagcc gtacgtcggg cgtgggtgat gttcagttgt tcggttcaca
    45241 gtacgcgatg cgtatctgga tgaacccgaa tgagctgaac aaattccagc taacgccggt
    45301 tgatgtcatt accgccatca aagcgcagaa cgcccaggtt gcggcgggtc agctcggtgg
    45361 tacgccgccg gtgaaaggcc aacagcttaa cgcctctatt attgctcaga cgcgtctgac
    45421 ctctactgaa gagttcggca aaatcctgct gaaagtgaat caggatggtt cccgcgtact
    45481 gctgcgtgat gtggcgaaga ttgagctggg tggtgagaac tacgacatca tcgcagagtt
    45541 taacggccaa cctgcttccg gtctggggat caagctggcg accggtgcaa acgcgctgga
    45601 taccgctgcg gcaatccgtg ctgaactggc gaagatggaa ccgttcttcc cgtcgggtct
    45661 gaaaattgtt tacccgtacg acaccacgcc gttcgtgaaa atctctattc acgaagtggt
    45721 taaaacgctg gtcgaagcga tcatcctcgt gttcctggtt atgtatctgt tcctgcagaa
    45781 cttccgcgcg acgttgattc cgaccattgc cgtaccggtg gtattgctcg ggacctttgc
    45841 cgtccttgcc gcctttggct tctcgataaa cacgctaaca atgttcggga tggtgctcgc
    45901 catcggcttg ttggtggatg acgctatcgt tgtggtagaa aacgtggagc gtgttatggc
    45961 ggaagaaggt ttgccgccaa aagaagctac ccgtaagtcg atggggcaga ttcagggcgc
    46021 tctggtcggt atcgcgatgg tactgtcggc ggtattcgta ccgatggcct tctttggcgg
    46081 ttctactggt gctatctatc gtcagttctc tattaccatt gtttcagcaa tggcgctgtc
    46141 ggtactggta gcgttgatcc tgactccggc tctttgtgcc accatgctga aaccgattgc
    46201 caaaggcgat cacggggaag gtaaaaaagg cttcttcggc tggttgaacc gcatgttcga
    46261 gaagagcacg caccactaca ccgacagcgt aggcggtatt ctgcgcagta cggggcgtta
    46321 cctagtgctg tatctgatca tcgtggtcgg catggcctat ctgttcgtgc gtctgccaag
    46381 ctccttcttg ccagatgagg accagggcgt atttatgacc atggttcagc tgccagcagg
    46441 tgcaacgcag gaacgtacgc agaaagtgct caatgaggta acgcattact atctgaccaa
    46501 agaaaagaac aacgttgagt cggtgttcgc cgttaacggc ttcggctttg cgggacgtgg
    46561 tcagaatacc ggtattgcgt tcgtttcctt gaaggactgg gccgatcgtc cgggcgaaga
    46621 aaacaaagtt gaagcgatta ccatgcgtgc aacacgcgct ttctcgcaaa tcaaagatgc
    46681 gatggttttc gcctttaacc tgcccgcaat cgtggaactg ggtactgcaa ccggctttga
    46741 ctttgagctg attgaccagg ctggccttgg tcacgaaaaa ctgactcagg cgcgtaacca
    46801 gctgcttgca gaagcagcga agcaccctga tatgctgacc agcgtacgtc caaacggtct
    46861 ggaagatacc ccgcagttta agattgatat cgaccaggaa aaagcgcagg cgctgggtgt
    46921 ttctatcaac gacattaaca ccactctggg cgctgcatgg ggcggtagct atgtgaacga
    46981 ctttatcgac cgcggtcgtg tgaagaaagt ttacgtcatg tcagaagcga aataccgtat
    47041 gctgccggat gatatcggcg actggtatgt tcgtgctgct gatggtcaga tggtgccatt
    47101 ctcggcgttc tcctcttctc gttgggagta cggttcgccg cgtctggaac gttacaacgg
    47161 cctgccatcc atggaaatct taggccaggc ggcaccgggt aaaagtaccg gtgaagcaat
    47221 ggagctgatg gaacaactgg cgagcaaact gcctaccggt gttggctatg actggacggg
    47281 gatgtcctat caggaacgtc tctccggcaa ccaggcacct tcactgtacg cgatttcgtt
    47341 gattgtcgta ttcctgtgtc tggcggcgct gtacgagagc tggtcgactc cgttctccgt
    47401 tatgctggtc gttccgctgg gggttatcgg tgcgttgctg gctgccacct tccgtggcct
    47461 gaccaatgac gtttacttcc aagtaggact gctcacaacc attgggttgt cggcgaagaa
    47521 cgcgatactt atcgtcgaat tcgccaaaga cttgatggat aaagaaggta aaggtctgat
    47581 tgaagcgacg cttgatgcgg tgcggatgcg tttacgtccg atcctgatga cctcgctggc
    47641 gtttatcctc ggcgttatgc cgctggttat cagtactggt gctggttccg gcgcgcagaa
    47701 cgcagtaggt accggtgtaa tgggcgggat ggtgaccgca acggtactgg caatcttctt
    47761 cgttccggta ttctttgtgg tggttcgccg ccgctttagc cgcaagaatg aagatatcga
    47821 gcacagccat actgtcgatc atcattgata caacatgtaa tcactaaggc cgcgtaagcg
    47881 gcctttttta tgcataacct acgaacatta aggagtaatt gaaccaccaa ctcaggatct
    47941 catacgaaaa ccagtattaa ccacgtataa aattcataaa aaatactgat tgttagttaa
    48001 tttatattaa gtagcgctaa tagatttaat aatccataat catttagagg ctattcttaa
    48061 ttatttgcgg taattcttta ttcattcctc ggttattacg tcatattcag agcaatcctg
    48121 gtattagtgt caccaatttc atctggcgat aatcctgaaa tgttatgaac agttcgagca
    48181 aactgctttt acctgctgcg ggttagtgct agtatgaaaa agtgagtcct gtcccgattc
    48241 cttcctaatt gtaatgtttc gtaataatgc gatgaaaacc tgcaaagagt ggcttatagt
    48301 taagctaaca aacgagaggg caagtctagg tcagtaagtt ttttccatcc cgaaaggtgt
    48361 ccgttagttc aaccactaag aaggggacgc gttatggatg aatactcacc caaaagacat
    48421 gatatcgcac agcttaagtt tctctgtgaa accctgtatc atgactgtct tgcaaacctt
    48481 gaagaaagca atcatggttg ggttaacgac ccaacctcgg cgatcaacct ccagttgaat
    48541 gaactgattg agcatattgc gaccttcgca cttaattaca aaattaagta taatgaagac
    48601 aataagctca ttgagcagat cgacgaatat ctggatgaca cctttatgtt gttcagcagt
    48661 tatggtatta atatgcaggc tcttcagaaa tggcggaagt caggtaatcg actattccgt
    48721 tgttttgtca acgcgacgaa agagaatcct gcgagtttat cttgttagaa ttattacaac
    48781 catgggtaga agtatgtccg aaaaaccttt aacaaaaacc gattatttaa tgcgtttacg
    48841 tcgttgccag acaattgaca cgctggagcg tgttatcgag aaaaataaat acgaattatc
    48901 agataatgaa ctggcggtat tttactcagc cgcagatcac cgcctcgccg aattgaccat
    48961 gaataaactg tacgacaaga tcccttcctc agtatggaaa tttattcgct aataaataat
    49021 tcgctttcgg tgctataacc ggctgtttat taagaatttt atactttttc gccatgaaga
    49081 cataccctat gtgatcttta tcacacagat gtaatgagaa cgttctcttc actgactttt
    49141 cgtcttactg tgttgccgca ttttcagcaa ccggaggtca gtaatgagca cagaaaaaga
    49201 aaagatgatt gctggtgagt tgtatcgctc ggcagatgag acgttatctc gcgatcgcct
    49261 gcgcgctcgt cagcttattc accgatacaa tcattccctg gcggaagagc acacattacg
    49321 ccagtaaatt ctcgctaatt tattcggtca ggtgacagag gcttatattg agccaacgtt
    49381 tcgctgtgat tatggctata acatttttct cggcaataat tttttcgcca acttcgattg
    49441 cgtgatgctt gatgtctgcc ctattcgcat cggtgataac tgtatgttgg caccaggcgt
    49501 tcatatctac acggcaacac atcccatcga ccctgtagca cgtaatagcg gtgctgaact
    49561 ggggaaaccc gtcaccatcg gtaataacgt ctggattggc ggacgcgcgg tcattaaccc
    49621 ttgtgtgacc attggtgata acgtcgtggt agcttcaggt gcagttgtca caaaaggtgt
    49681 cccggacaac gttgtcgtgg gcggtaatcc agccagaata attaaaaaat tgtaatcggt
    49741 ttttcgcaac tgttatgcaa aattgtggta gatctgttac ttcccctcta ctattcccac
    49801 gttaaaatag ggtgttccct ggaaagttgc agataccacg aaggcaaacg atgaccgaaa
    49861 tacaacgcct gctgaccgaa acgattgagt ctctgaatac ccgcgaaaaa cgcgacaaca
    49921 aaccccgctt tagtatcagt tttatccgta aacatccggg gctgtttatc ggtatgtacg
    49981 ttgctttttt tgccaccctg gcggtgatgt tgcagtccga aacgctgtca ggctctgtct
    50041 ggctactggt tgtattattt atcctgctta atggtttctt ctttttcgat gtctacccac
    50101 gctaccgcta tgaagatatc gacgtgctgg atttccgcgt ttgctataac ggcgaatggt
    50161 acaacacgcg ctttgtacct gccgcgctgg ttgaagccat cttgaactct ccgcgtgtcg
    50221 cggatgttca taaggaacaa ctgcaaaaaa tgatcgtccg taaaggtgaa ctgtcttttt
    50281 acgatatttt taccctcgct cgcgccgaat caacatctta agttagggtt acataccagg
    50341 cgtaaagctc tgcgcctggt caaatgacaa tgatcgtttc cacccatcac ttcatgaaat
    50401 accagctcta cctccttatc tcctgccagc ctttttccac aatcagatat actttcccta
    50461 cactgtgtta ataaggatat gctggtgaga acacgacatc tggtcggcct tatttcggga
    50521 gtactgattc tttcagtatt gctgcctgtc ggcttaagca tctggctggc ccatcagcag
    50581 gtagaaacat cgtttattga agagctggat acctattcct cccgcgtcgc tattcgagcc
    50641 aataaggtgg cgacacaagg gaaagatgcg ctgcaggagc tggaaagatg gcaaggcgct
    50701 gcctgtagcg aagcccatct catggaaatg cgtcgggtat cttacagtta tcgctatatt
    50761 caggaagtgg tttatatcga taacaacgtt ccccagtgtt cgtctctgga gcatgaaagt
    50821 ccgcccgata cctttcccga gctaggtaaa atttcgaaag atggttatcg tgtctggtta
    50881 acatcgcata acgatttagg cattatccgt tacatggtcg ccatgggaac ggcacattat
    50941 gtcgtcatga tcgaccccgc ttcctttatt gatgtcattc cctatagttc atggcaaatt
    51001 gatgccgcca ttattggcaa tgcccataac gttgtcataa ccagcagcga tgaaattgct
    51061 cagggaatta ttaccaggct acaaaaaaca cccggtgagc atatcgaaaa taatgggatc
    51121 atttacgata tactgccctt cctggagatg aatatttcga tcatcacctg ggcttcaacg
    51181 aaaatgttgc agaaaggctg gcatcggcaa gtctttattt ggttaccgct cgggttggtg
    51241 attggcctgc tggcagcgat gtttgtgctg cgtattttgc gccgtatcca atcaccgcat
    51301 catcggctgc aggatgctat cgaaaatcgt gatatttgcg tgcactatca gccgattgtc
    51361 tccttagcca atggcaaaat tgtcggtgct gaggcactgg cgcgctggcc gcagacagac
    51421 ggtagttggt tgtcaccaga tagttttatt ccgctggcac agcaaacggg cctttctgag
    51481 ccattgacgc tactgattat aagaagcgtc tttgaagata tgggcgactg cttgcgtcag
    51541 catccacagc agcatatttc gatcaatctt gaatccaccg tgctcacctc ggaaaaaatc
    51601 ccgcaattgc tgcgtgaaat gatcaatcac tatcaggtta atcccagaca gatcgcgctt
    51661 gaactcactg aacgcgagtt tgccgatccg aaaaccagcg ccccgataat ttctcgctac
    51721 cgggaggcgg gccatgaaat ttatcttgat gattttggta cggggtattc aagtttaagt
    51781 tatttacagg atctggatgt cgacattctg aagatcgata aatctttcgt tgatgcgctg
    51841 gaatataaaa atgtcacgcc gcatatcatc gaaatggcaa aaacactgaa actgaaaatg
    51901 gtagcggagg gaatcgaaac cagtaaacaa gaagagtggc tacgccagca tggcgtgcac
    51961 tacggtcagg gctggctcta cagcaaggca ttaccgaaag aagatttctt acgctgggcc
    52021 gagcaacatt tgtgaatttt tgggctgacg gcacgccgca cagcccgtca cgctacctat
    52081 tcgtcgatga ttgactcgaa tccgccatag atcattcgct tgccgtcaaa cggcatggac
    52141 tcgccgaact ctttcatccg tgggtccgac atcatctttt gattagctgc gtcgcggacc
    52201 tcttttgaag ggtattcaat ccagctaaag acaacctctt cattctcttc cgctttcacc
    52261 gccatacgaa aatcggtcac tttgccatcc ggcacatcgc tggcccagca ttcgacaata
    52321 cgaagcgcgc caaactcttt aaacaatggc gcggccttag cggccatttc ccgataggca
    52381 tcctttttat cggcaggaac ggcaaccaca aaaccatcaa catacttcat cagacatacc
    52441 tccgaagcgt actgcgcagc cagacaattc ttgcgacgtc agttgtggtt aaagtttagc
    52501 catgctggga ttttttgacg aaacggtggg aggcagaaat gggtgggggc cctgccagct
    52561 acatcccggc acacgcgtca tctgccttgg ctgcttcctt ccggacctga cctggtaaac
    52621 agagtagcat tgcgggagaa ccaacagagc ccccattgag agcgttgaaa accaacgcgc
    52681 aggcgcatta tcactgctgc cccggctaat tgcaacccat tgcgtgccag atgctggttt
    52741 cttgcgcaat gcgacttcac tcgggcgttt ttccagatta tgctgaaaaa ttacctcagg
    52801 aagaaaagat ggaaaaagaa gattcatttc cccaacgcgt ctggcaaatc gtcgccgcta
    52861 ttcccgaagg ctatgtcacc acttacggtg atgtggcgaa actggcggga tcgccccgcg
    52921 ccgcgcgcca ggtgggcggt gtgttaaagc gtctccctga aggcagcacc ttaccctggc
    52981 accgggtggt taatcgccat ggcacaattt cgctaaccgg gccggattta cagcgtcagc
    53041 gacaggcatt actggcagaa ggtgtgatgg tatcgggaag cgggcaaatc gacttgcagc
    53101 gttatcgctg gaactactaa ccctctccgg
//
LOCUS       DAJTPG010000015        52544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTPG010000015 DAJTPG010000000
VERSION     DAJTPG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..185
                     /locus_tag="OO230_000954"
     CDS             <1..185
                     /locus_tag="OO230_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5690809.1"
                     /translation="FQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
     gene            complement(223..1122)
                     /gene="nhaR"
                     /locus_tag="OO230_000955"
     CDS             complement(223..1122)
                     /gene="nhaR"
                     /locus_tag="OO230_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5690810.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1188..2354)
                     /gene="nhaA"
                     /locus_tag="OO230_000956"
     CDS             complement(1188..2354)
                     /gene="nhaA"
                     /locus_tag="OO230_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5690811.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            2940..3092
                     /gene="mokC"
                     /locus_tag="OO230_000957"
     CDS             2940..3092
                     /gene="mokC"
                     /locus_tag="OO230_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5690812.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3196..4326)
                     /gene="dnaJ"
                     /locus_tag="OO230_000958"
     CDS             complement(3196..4326)
                     /gene="dnaJ"
                     /locus_tag="OO230_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5690813.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4415..6331)
                     /gene="dnaK"
                     /locus_tag="OO230_000959"
     CDS             complement(4415..6331)
                     /gene="dnaK"
                     /locus_tag="OO230_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5690814.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6708..7112
                     /locus_tag="OO230_000960"
     CDS             6708..7112
                     /locus_tag="OO230_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5690815.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7138..7851
                     /gene="msyB"
                     /locus_tag="OO230_000961"
     CDS             7138..7851
                     /gene="msyB"
                     /locus_tag="OO230_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5690816.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8000..8566
                     /gene="satP"
                     /locus_tag="OO230_000962"
     CDS             8000..8566
                     /gene="satP"
                     /locus_tag="OO230_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5690817.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8601..9188)
                     /gene="mog"
                     /locus_tag="OO230_000963"
     CDS             complement(8601..9188)
                     /gene="mog"
                     /locus_tag="OO230_000963"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5690818.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9303..10256)
                     /gene="tal"
                     /locus_tag="OO230_000964"
     CDS             complement(9303..10256)
                     /gene="tal"
                     /locus_tag="OO230_000964"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5690819.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10535..11965
                     /locus_tag="OO230_000965"
                     /pseudo
     CDS             10535..11965
                     /locus_tag="OO230_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12035..12811
                     /gene="yaaA"
                     /locus_tag="OO230_000966"
     CDS             12035..12811
                     /gene="yaaA"
                     /locus_tag="OO230_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5690820.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(12964..13260)
                     /locus_tag="OO230_000967"
     CDS             complement(12964..13260)
                     /locus_tag="OO230_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5690821.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13474..14760)
                     /gene="thrC"
                     /locus_tag="OO230_000968"
     CDS             complement(13474..14760)
                     /gene="thrC"
                     /locus_tag="OO230_000968"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5690822.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPTDFSALRKLMMNHQ"
     gene            complement(14761..15693)
                     /gene="thrB"
                     /locus_tag="OO230_000969"
     CDS             complement(14761..15693)
                     /gene="thrB"
                     /locus_tag="OO230_000969"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5690823.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15695..18157)
                     /gene="thrA"
                     /locus_tag="OO230_000970"
     CDS             complement(15695..18157)
                     /gene="thrA"
                     /locus_tag="OO230_000970"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5690824.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18183..18302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18238..18303)
                     /gene="thrL"
                     /locus_tag="OO230_000971"
     CDS             complement(18238..18303)
                     /gene="thrL"
                     /locus_tag="OO230_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5690825.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18517..19203)
                     /locus_tag="OO230_000972"
     CDS             complement(18517..19203)
                     /locus_tag="OO230_000972"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5690826.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19603..19743)
                     /locus_tag="OO230_000973"
     CDS             complement(19603..19743)
                     /locus_tag="OO230_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690827.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19839..20555
                     /gene="arcA"
                     /locus_tag="OO230_000974"
     CDS             19839..20555
                     /gene="arcA"
                     /locus_tag="OO230_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5690828.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20614..21966)
                     /gene="creD"
                     /locus_tag="OO230_000975"
     CDS             complement(20614..21966)
                     /gene="creD"
                     /locus_tag="OO230_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5690829.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22024..23448)
                     /gene="creC"
                     /locus_tag="OO230_000976"
     CDS             complement(22024..23448)
                     /gene="creC"
                     /locus_tag="OO230_000976"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5690830.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23448..24137)
                     /gene="creB"
                     /locus_tag="OO230_000977"
     CDS             complement(23448..24137)
                     /gene="creB"
                     /locus_tag="OO230_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5690831.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24150..24623)
                     /gene="creA"
                     /locus_tag="OO230_000978"
     CDS             complement(24150..24623)
                     /gene="creA"
                     /locus_tag="OO230_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5690832.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24834..25703
                     /gene="robA"
                     /locus_tag="OO230_000979"
     CDS             24834..25703
                     /gene="robA"
                     /locus_tag="OO230_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5690833.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25700..26347)
                     /gene="gpmB"
                     /locus_tag="OO230_000980"
     CDS             complement(25700..26347)
                     /gene="gpmB"
                     /locus_tag="OO230_000980"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5690834.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26399..26919
                     /gene="yjjX"
                     /locus_tag="OO230_000981"
                     /pseudo
     CDS             26399..26919
                     /gene="yjjX"
                     /locus_tag="OO230_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27004..27330)
                     /gene="trpR"
                     /locus_tag="OO230_000982"
     CDS             complement(27004..27330)
                     /gene="trpR"
                     /locus_tag="OO230_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5690835.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27420..29357)
                     /gene="sltY"
                     /locus_tag="OO230_000983"
     CDS             complement(27420..29357)
                     /gene="sltY"
                     /locus_tag="OO230_000983"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5690836.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29568..31235
                     /gene="ettA"
                     /locus_tag="OO230_000984"
     CDS             29568..31235
                     /gene="ettA"
                     /locus_tag="OO230_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5690837.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31342..31509)
                     /locus_tag="OO230_000985"
     CDS             complement(31342..31509)
                     /locus_tag="OO230_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690838.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31543..32775)
                     /gene="nadR"
                     /locus_tag="OO230_000986"
     CDS             complement(31543..32775)
                     /gene="nadR"
                     /locus_tag="OO230_000986"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5690839.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32796..34178)
                     /gene="radA"
                     /locus_tag="OO230_000987"
     CDS             complement(32796..34178)
                     /gene="radA"
                     /locus_tag="OO230_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5690840.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34227..35195)
                     /gene="serB"
                     /locus_tag="OO230_000988"
     CDS             complement(34227..35195)
                     /gene="serB"
                     /locus_tag="OO230_000988"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5690841.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35301..35945
                     /locus_tag="OO230_000989"
     CDS             35301..35945
                     /locus_tag="OO230_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5690842.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            35973..36988
                     /gene="lplA"
                     /locus_tag="OO230_000990"
                     /pseudo
     CDS             35973..36988
                     /gene="lplA"
                     /locus_tag="OO230_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37020..37283
                     /locus_tag="OO230_000991"
     CDS             37020..37283
                     /locus_tag="OO230_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5690843.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37444..38163)
                     /gene="deoD"
                     /locus_tag="OO230_000992"
     CDS             complement(37444..38163)
                     /gene="deoD"
                     /locus_tag="OO230_000992"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5690844.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38220..39443)
                     /gene="deoB"
                     /locus_tag="OO230_000993"
     CDS             complement(38220..39443)
                     /gene="deoB"
                     /locus_tag="OO230_000993"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5690845.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39495..40817)
                     /gene="deoA"
                     /locus_tag="OO230_000994"
     CDS             complement(39495..40817)
                     /gene="deoA"
                     /locus_tag="OO230_000994"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5690846.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(40944..41723)
                     /gene="deoC"
                     /locus_tag="OO230_000995"
     CDS             complement(40944..41723)
                     /gene="deoC"
                     /locus_tag="OO230_000995"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5690847.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            41981..43522
                     /locus_tag="OO230_000996"
     CDS             41981..43522
                     /locus_tag="OO230_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5690848.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43494..44357
                     /locus_tag="OO230_000997"
     CDS             43494..44357
                     /locus_tag="OO230_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5690849.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44571..45353)
                     /locus_tag="OO230_000998"
     CDS             complement(44571..45353)
                     /locus_tag="OO230_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5690850.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45350..46423)
                     /locus_tag="OO230_000999"
     CDS             complement(45350..46423)
                     /locus_tag="OO230_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5690851.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46545..46706)
                     /locus_tag="OO230_001000"
     CDS             complement(46545..46706)
                     /locus_tag="OO230_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5690852.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46823..47428)
                     /gene="osmY"
                     /locus_tag="OO230_001001"
     CDS             complement(46823..47428)
                     /gene="osmY"
                     /locus_tag="OO230_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5690853.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47821..49410)
                     /gene="prfC"
                     /locus_tag="OO230_001002"
     CDS             complement(47821..49410)
                     /gene="prfC"
                     /locus_tag="OO230_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5690854.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49501..50178)
                     /gene="yjjG"
                     /locus_tag="OO230_001003"
     CDS             complement(49501..50178)
                     /gene="yjjG"
                     /locus_tag="OO230_001003"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5690855.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50193..50639)
                     /gene="rimI"
                     /locus_tag="OO230_001004"
     CDS             complement(50193..50639)
                     /gene="rimI"
                     /locus_tag="OO230_001004"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5690856.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50608..51021)
                     /gene="holD"
                     /locus_tag="OO230_001005"
     CDS             complement(50608..51021)
                     /gene="holD"
                     /locus_tag="OO230_001005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5690857.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51124..52155
                     /gene="rsmC"
                     /locus_tag="OO230_001006"
     CDS             51124..52155
                     /gene="rsmC"
                     /locus_tag="OO230_001006"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5690858.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52432..52518
                     /locus_tag="OO230_001007"
     tRNA            52432..52518
                     /locus_tag="OO230_001007"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52466..52468,aa:Leu,seq:cag)
BASE COUNT        12493 a        14237 c        13171 g        12643 t
ORIGIN      
        1 gctttcaact ctccaatgat ggtacgaatg aaatccagta tggtaaaagc aattactact
       61 cttttcagca tccccatgaa ggaagtaatc agattcctct atttattcgc ccgcgtacat
      121 atggaaacaa tgtctcaagc gggcaaataa tgagccgagt caaaatcgtg gtaatgtaca
      181 actaatctat ctcccccacc ttaactttgg gggagctgcc gattaacgca ccgctggact
      241 aaaaagcgca gaataatccg tattgcagat tcgctgtacc gccgggtgct gaatcatccg
      301 ctcagcaaaa atagcatggt actcttccat cacattctcg acgcgaccaa tttctacgac
      361 agttttatcg gcataaaagt catatgcata aagcgttggg gcaacgaaga ttgcattgtg
      421 catcgcacca aaagctttca tcaaagcggc atcatcaaac tcgccgagga tttctacgtt
      481 taatccctgg gagttaaacc agttaagcaa tttgcgccct aacattgaac gtcgcccagg
      541 aatcaaaagt cgccgttctt ccagacaagc cgggaacggt ttttctggtg gtggatttgt
      601 acaccagaaa ctcacgccac attcgccaat tctcacggag aacaggcctt cctgctgcgt
      661 agagtctatc ggacagtcag aaatgatcat atccagttta tgctgactta attgctccag
      721 cagcatttcg tgggtggatt cgaagcagcg aagatgaatg ggctcgcctt ctaccactgc
      781 ggcgttaagt acgctactga ccaggcgttt ggaaagtgca tcagccacgc caacgtcaaa
      841 caataaattg gattctttgc gatagttcac aatatccagc atttcctggc ttaaggtgaa
      901 cattttatcg gcatagcgat agaccagttc tcccagctcg ctgggttcga gaccacgtcc
      961 cttgcgttta aataatttgc cttgcaggcg ctcttccagc gctcgaatct gtccggtaat
     1021 ggtttgtgga gttaaataaa gcgcctccgc tgcgccaacc acggaacctt ctttatagac
     1081 atgccagaag taatacaagt ggttgtaatt gatatgagac atgctcattt ctctccctga
     1141 taacaatgaa aagggagccg tttatggctc cccggtaaac cgtcctgtca aactgatgga
     1201 cgcaaacgaa cgcgtaacca gctgtatcca attaccgccg aagagataga accgaccagg
     1261 ataccgagtt tcgcccagtt aatcagttct ggatctacgc taccaaaggc caggctggca
     1321 ataaagatag acatagtaaa accgataccg cacaggatcc ccaccaccat aatttgctga
     1381 taagtcgttc cctcaggcag atgcgccagt ttcaaacgca gcgccaacca gcagaacaga
     1441 ctaatcccca gcggtttgcc aatcagcaag ccagcgatga tccccaatgg cagaatggag
     1501 gtcaagccat ccagcgtgac gccttgcagt gaaacgccag cattagcaaa tgcaaacagc
     1561 ggcaaaatca gatacgccac ccacgggtgc aacacatgct ccagtcgctt cgctggagaa
     1621 cgcccatgct tctctttcaa aggaataaag aagccgacaa ttacccccgc cagagttgcg
     1681 tgaacccccg atttcaacac cgcagtccac aacaccacgc caacaagaat atagacgccc
     1741 gtgcggcgta caccacacag attcaatacc gcgagtaccg caattgctac agccgcgacg
     1801 ccaagggagg ccatcgataa gtcattagtg tagaacaatg cgatgataat gatggcccca
     1861 agatcgtcga taatagccag agccatcaaa aagatcttca gcactaacgg aacacgactt
     1921 cccaacagcg ccagtacacc aagtgcaaaa gcaatgtcag tagccgccgg gatcgcccac
     1981 ccttcgcggg taatcggatc ggcatagtta aaagccagat agagtaatgc cggcacaatc
     2041 atcccaccaa tagcggcgat aactggaaat gcggcctggc gtaagctggc tagcgatcct
     2101 tgcatcagtt cacgtttaac ttccagaccg actaacagga aaaataccgc catcagcgca
     2161 tcatttatcc ataacagcat gtttttgttg atttcgagtg aaccaacccg gagctgaacc
     2221 ggcgtctcca gaaagtcgtg ataccatcca ctggttgcgc cgctgttggc cataatcatc
     2281 gccaggatag cggcaatgat aagaataatg cctcccgagg catcactgct aaagaatcga
     2341 tgcagatgtt tcacttttta tttctctttc aggtgaatag atcgttttta ctattttacc
     2401 ccgcacgaat catcgttaaa atcagattat agtgtaggaa taattcgttt tttacgagcc
     2461 attgaagaat ttgcgccatc agcggtgaga tgcgtttttg acggctgtaa gcgctcacga
     2521 gtgaccctaa gcccagataa tcatagctta aggtgaggat tcctgaggga ttaattagga
     2581 aaagtaataa ttaatttgtt gtcagtcggc gatgcttgtt aggccaatat ttcaaaaata
     2641 atccagcttg tcgtcaataa ttgcgatgaa gagataaatt aaagttaaat aattaaatca
     2701 ttaaaaaatt acgccacaaa aatgcgaggc gtcttcagga ttagaatcga taaaacaaaa
     2761 tgggttaaag agagcgatat aatagcggcg ggtgcttgag gctgtctgtc tcaggcatta
     2821 gctgaacggc agatagagaa aagccccgag tgatatttta ccatcaaccc gaggcctcct
     2881 atatgctgaa cacatgtaga gtgcctctta ctgaccgtaa ggtcaaggag aagagagcaa
     2941 tgaagcagca taaggcgatg attgtcgccc tgatcgtcat ctgtatcacc gccgtagtgg
     3001 cggcgctggt aacgagaaaa gacctctgtg aggttcacat ccgaactggc cagacggagg
     3061 ttgctgtttt cacggcttac gaatccgagt aagagcaacg gcggggagtg atccccgcca
     3121 ctttacaggt gctcgcatat cttcaacgca ccctattttt acccaggcct gcccacgggc
     3181 aggcttttgg ggaggttagc gggtcaggtc gtcaaaaaac ttcttcacac catcaaagaa
     3241 gctctttgag cgcgggctgt tgtgctcgcc ggttgggcca ccgaagcttt cttgcagctc
     3301 ttgcagcagc tgtttctgct tttcgttcag gcctaccggt gtttcgacga caacgcggca
     3361 cagcaaatca ccctgtgcgc caccgcggac agacttgacg cctttaccgc gcatacggaa
     3421 tagcttaccg gtctgggttt cgccaggcac tttcagtttg acgcgaccat caagggtcgg
     3481 tacttcgatt tcgccaccca gcgccgccat agcgaagttg atcgggactt cgcaatacag
     3541 gttgttgcct tcacgctcga aaatcgggtg ctgtttaacc tgaacctgaa cgtacagatc
     3601 gcctgccggt gcgccatgct cgcccgcttc accttcgccc gcaagacgga tgcggtctcc
     3661 agtgtccacc cctgccggga ttttaacgga cagcgttttg ctgcgctcaa cacgaccatg
     3721 accatgacat ttgttgcacg gatctttgat cagcgtaccg cggccctgac agtgtggaca
     3781 ggtctgctgt acagcgaaga atccctggcg catctgcacc tgaccagaac catgacaggt
     3841 cggacaggtc tgcggctgtg tacctggttt tgcaccgcta ccgtggcaaa cgtcacactc
     3901 ttccagagtc ggaatgcgga tctctttggt cacgccacgt acagcttctt cgagggtgag
     3961 ctccatgtta tagcgtaaat cagcaccgcg cgccgcacgt tgacgaccac gtccgccgcc
     4021 aaaaatatcg ccgaaaacgt caccaaaaat atcgctgaag tctgcgccgc cgccaaaacc
     4081 accgccgccc atgcctcctt gctcaaacgc agcatgacca tactgatcgt atgccgcacg
     4141 tttttgcgag tcggtcagaa cttcataagc ttccttgatc tctttaaatt tcgcctcggc
     4201 ctctttgtca ccctggttac ggtccgggtg gtatttcatg gccaggcgtt tgtaggcctt
     4261 tctgatttca cgctcttccg ctgttttgga aacgcctaaa atctcgtaat aatcttgctt
     4321 agccatcttt tttaaattgc ccctagatga atgcacgggc ggagaggaaa ttccccttcg
     4381 cccgtgtcag tataattacc cgtttatagg gcgattattt tttgtctttg acttcttcaa
     4441 attcagcgtc gacaacatcg tcatctttcg cgttgtttgc agaagcatca gcaccggcag
     4501 tctgctgctg ggcatgttgc tgctgggcga tttccatcag tttctgggaa acctgtgcca
     4561 gttcctgcat tttcgcttcg atagcggctt tgtcttcacc tttcagagca gtttccagtg
     4621 cagtcagcgc agactcgata gcagttttgt cgtcagccgg cagtttgtcg cctgcttctt
     4681 caacctgctt acgggtgctg tgcagcagat ggtcgccctg gttgcgagtc tgtaccagct
     4741 cttcaaactt acggtcagct tcggcgttag cttctgcgtc gcgtaccatt ttctggattt
     4801 catcttcgtt cagaccagaa gaagccttga tggtgatctt ctgctcttta ccgctgtttt
     4861 tatctttcgc ggaaacgtgc aggataccgt cagcatcgat atcgaaggta acttcgatct
     4921 gcggcatgcc gcgcggtgcc gggttgatac catctaggtt gaactgaccc agagatttgt
     4981 tatcagccgc acgtttacgt tcaccctgca gcacatggat ggttaccgca gactggttgt
     5041 cttcagcggt agagaacacc tggctgtgct tggtcgggat agtggtgttt ttcgcgatca
     5101 gcgtcgtcat cacaccgccc atggtttcga tacccagaga cagcggggta acgtccagca
     5161 gcagtacgtc ttttacgtca ccagtcagaa caccaccctg aacagcagca ccgattgcta
     5221 cagcttcgtc cgggttaacg tctttacgcg gctctttacc aaagaactca gcaactttct
     5281 tctgaaccat tggcatacga gtctgaccac caacgaggat aacgtcgtcg atatcagata
     5341 cggacaggcc agcatcctgc agtgcaactt tcagcggctc aatggaacgg tttaccagat
     5401 cttcaaccag gctttccagt ttcgcacgag tcactttgat gttcatgtgt ttcggaccgg
     5461 tcgcgtctgc agtgatgtat ggcaggttaa cgtcggtctg ctgagcggaa gacagttcga
     5521 ttttcgcttt ttctgccgct tctttcaggc gctgcattgc cagcggatcg ttgcgcaggt
     5581 caatgccctg atctttcttg aattcttcaa ccagatagtt gatcagacgg ctgtcgaagt
     5641 cttcaccacc caggtgggta tcaccgttgg ttgccagaac ttcgaaggtt ttttcgccgt
     5701 caacttcgtc gatttcgata atagaaatat cgaaagtacc accccccagg tcataaaccg
     5761 cgatagtacg gttgccagtg cctttgtcca gaccgtaagc cagcgcagct gcggtcggtt
     5821 cgttgatgat acgttttact tccagaccag cgatacggcc tgcgtctttg gttgcctgac
     5881 gctgagcatc gttaaagtat gccggtacgg tgataacagc ttcagttacc ggttcaccca
     5941 ggtaatcttc agcggttttc ttcatttttt tcagcacttc agcagaaatc tgcggcggtg
     6001 ccattttctg gcctttaact tcgacccatg cgtcgccgtt atcagcagca ataattttga
     6061 acggcatgat ggaaacatca cgctgtactt cttcgtcctg gaagcggcgg ccaatcaggc
     6121 gtttaatcgc aaacagggtg ttttgcgggt tcgtcactgc ctgacgttta gccggctgac
     6181 caaccagagt ttcaccatcc tgggtatagg caatgataga aggcgtggtg cgatcgcctt
     6241 cggcgttctc cagcacgcga ggaatggtgc catccataat cgctacacaa gagttggtag
     6301 tacccaggtc gataccaatt attttaccca tctaaacgtc tccactatat attcggtcat
     6361 catgtggttg tgagtctgta ataggggcga aacgtctggt ttcaactgcc caatttcatt
     6421 tttttgcaga ctcactcact gcggttgact actaaatggg gtcgtaaccc acgtcatcaa
     6481 gggggagatg caaaaatttt ttgtgctttt gcgcagaaat ttcgcgatat ggcaggacaa
     6541 gacgtaaaat tgacagcaat tttattcata taaagaatga atcgcagcca aggcgttcat
     6601 caaaaaattg taataaaaag aaaagattac gtgcctgaat cttctcttta tcagcagtaa
     6661 actagtgggt attcatcccc ctacctcttc ccactaagag aatccttatg aaatccgttt
     6721 ttacgatttc cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg
     6781 accataaact cctcggcgtc attgcaatgc cgcgtaacga aaccaacgat ctggcgctga
     6841 aacttcctgt ttgtcgcatt gtgaaacgca tacaactctc tgccgaccat ggcgatttac
     6901 agttaagtgg tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag agcctgaata
     6961 ttccttcaga aataaaagaa gggcaaacca ctgactggat caacattaac agcgataacg
     7021 acaataaacg ctgcgtctca aaaatcacct tttccggtca tacggtgaac tcatcggata
     7081 tggccacgct gaaaattatc ggcgacgact aacgccagaa tatagaagcc acaaaaaatg
     7141 aatgttaatt acctgaatga ttcagatctg gattttcttc agcattgtag tgaggaacag
     7201 ttggcaaatt tcgcccgatt gctcacccat aatgaaaaag gcaaaactcg cctctccagc
     7261 atactgatgc gcaacgaact gtttaaatcg atggaagggc atcccgagca acatcgccgc
     7321 aactggcagc tgattgccgg agaattacag cattttggtg gcgatagtat cgccaacaaa
     7381 ctgcgcggac acggtaaatt gtatcgggcc attttgctcg atgtttcaaa gcgattgaag
     7441 ctgaaagccg acaaagagat gtctacgttt gaaattgagc aacagttact ggaacaattt
     7501 ctgcgtaata cctggaagaa aatggacgag gaacataagc aggagtttct gcacgcggtc
     7561 gatgccaggg tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga caaattattg
     7621 gcaaaaggtg tgtcgcattt gctttccagc caactgaccc gcattttacg cacccacgca
     7681 gcaatgagcg tacttgggca tggtttgctg cgcggcgcgg ggctgggagg ccctgtgggc
     7741 gcagcattaa atggggttaa agcggtcagc ggcagcacct atcgcgtgac gattccagcc
     7801 gtactgcaaa tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg ataccggaaa
     7861 ccggggaaaa ttttcatttt cccctcaaaa gatcgtagac actgccccac tggctgatta
     7921 ttatgccgcg ccctgaaaac actacagtta ttcagggaaa ttatttcacc attcattcga
     7981 tgatgatttt tgaggaatta tgggcaacac taagttggct aatccggcac cgctgggcct
     8041 gatgggcttc ggcatgacca ccattctgct taacctgcac aacgtgggtt atttcgctct
     8101 ggacggtatt attcttgcca tgggcatttt ctacggcggc atcgcgcaaa tttttgctgg
     8161 tctgctggag tacaaaaaag gcaacacttt cggtttaacc gcattcacct cttacggttc
     8221 tttctggctg acgctggttg cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc
     8281 aaatgcacag ttccttggtg tctacctggg tctgtggggc gtatttacgc tgtttatgtt
     8341 cttcggcact ctgaaaggcg cgcgcgtcct gcaattcgtt ttctttagcc tgaccgtgct
     8401 gtttgccctg ctggcgatcg gtaacattgc cggtaatgcc gcgatcatcc actttgccgg
     8461 ctggattggt ctgatctgcg gtgccagcgc aatctatctg gcgatgggtg aagtgctgaa
     8521 cgagcagttt ggtcgcaccg ttctgccgat tggtgaatcc cactaattgt cttgagatcc
     8581 ccccgctcgg ggggattttt ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat
     8641 gcagcaacca cttctggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac
     8701 ggtacgctgg caaaaatacc atgcaccaca acgttaccct cagcgtcctt cacaccttcc
     8761 agcgtctctt taatagactt cggctgaccg ggtaagttaa ggatcagcgc ctgtttgcga
     8821 atcacgccca cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc
     8881 atctgttcac caaagccagg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt
     8941 acgtcacgac gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca
     9001 tccaccagct cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt
     9061 tccagttcaa acggcgtggt tagcgccgat gtcagccatt cttccagcgc tggaatgcct
     9121 ttatcctgat aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa
     9181 gtattcatca agatttttcc gtttaaacaa tctcgttaaa cagaatgata cactgcgaag
     9241 ggagtgacag acaggcttca gaagaggtag cgtgaccgac ttcccggtca cgctaagaat
     9301 gattacagca gatcgccgat cattttttcc agtttttcct ggtcaatagc aaacttacgg
     9361 ataccttccg ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg
     9421 gactcagtga tacgcgccgg acgcgctttc acttcgccgg tgtaagacag tttacgttcg
     9481 atagcccctt cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtca
     9541 cagcctgcca gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt
     9601 tcataaccgt gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct
     9661 tctgccggag cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca
     9721 acaaacggcg agatcaggaa cacgcccgcc tccgcacaag cacgagcctg agcgaaggag
     9781 aacagcaggg tcaggttaca gttgatacct tctttttcca gctgttctgc agcacggata
     9841 ccctgccagg tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg
     9901 tagagtttga tcaggcgttt tgctttcgca attgacgctt cagtgtcata ggaaagacgc
     9961 gcatcaactt cagttgagat acggcccgga accagtttca ggatttccag accaatattt
    10021 actgccagtt tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc
    10081 gcccaggcga cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga
    10141 atgagagaag ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc
    10201 ccagtgtcgg ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata
    10261 gtatttctct ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg
    10321 acgcaacgtc agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt
    10381 tctgacactg cgttgatatc gcccaccatt tttatacaaa acctcatgta tgctacgcag
    10441 aagttatcaa gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg
    10501 aaagtcatcg cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa
    10561 cagcgtcctt tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac
    10621 ttttcgcacc ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa
    10681 tagcattcat ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc
    10741 ggcgcgcgtg ggtagcggca acctggccgg cgttgcgctg gcaattaccg ccggtggacc
    10801 aggtgccgtc ttttggatgt gggttgctgc gtttatcggc atggcgacct cgtttgccga
    10861 atgttccctt gcacaacttt ataaagaacg tgacgtcaat gggcagtttc gtggcggacc
    10921 ggcatggtat atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt
    10981 tttgctcatc gcctacggca taattttcag cggagttcag gcgaacgccg ttgcccgagc
    11041 cctgagtttt tcttttgatt ttcctccgct ggtgacgggc attattctcg ctgtctttgc
    11101 tctgctggca atcactcgtg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc
    11161 gttgatggcg ataatctggg tactgaccag cctggtgatt tgcgtaataa atatcgggca
    11221 acttccccac gtcatttggt ctatttttga gagtgctttt ggctggcagg aagcggcagg
    11281 cggcgcggcg ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt
    11341 ttccaatgag gtgggaatgg gatcgacgcc aaacgcggca gcggcagctg catcctggcc
    11401 tccgcatccg gcagcacaag ggattgtcca gatgattggc atttttatcg acaccctggt
    11461 catctgtacg gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc
    11521 gctggaaggt attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctagggtgc
    11581 tgaatttgtt accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat
    11641 ttatgccgaa aacaatatct tctttttacg cctgaacaac cctaaagcga tctggtgttt
    11701 gcggatctgc accttcgcaa cggtcatcgg cggcaccttg ctaagccttc cgctgatgtg
    11761 gcaactggca gatatcataa tggcctgcat ggctattacc aatttgaccg ccattttact
    11821 gctctcgcct gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt
    11881 gcgcccggtg tttgatccgt tgcgttatcc ggatatcggt cgccagcttt ctccggacgc
    11941 gtgggatgat gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc
    12001 taaagtcggc ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa
    12061 aacgcttgat taccaaagcc cgttgaccac cacgcgctat acgctgccgg agctgttaga
    12121 caattcccag cagttgatcc atgaagcgcg gaaactgacg cctccgcaga ttagcacgct
    12181 gatgcgcatc agcgacaaac tggcgggtat caacgccgct cgctttcatg actggcagcc
    12241 agatttcacg ccggcgaatg cccgccaggc gattctggcg tttaaaggtg atgtctacac
    12301 cggcttgcag gccgaaacct tcagcgaaga cgatttcgat tttgcccaac agcatttgcg
    12361 aatgctttcc ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct
    12421 ggaaatgggg atccgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga
    12481 tatcatcacc aacaagctga acgaggcgct cgcagcacaa ggcgataatg tggtgattaa
    12541 tctggcgtca gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat
    12601 caagccggtg ttcctcgatg agaagaacgg caaatttaag atcatcagct tctacgctaa
    12661 gaaagcacgc ggtctgatga gtcgtttcat tattgaaaat cggctgacca aaccagagca
    12721 actgactggt tttaatagcg aaggttactt ctttgatgaa gattcctcca gcaatggcga
    12781 actggtgttt aaacgctacg agcagcgtta agtttgtagt caatgccgga tgcggcgtga
    12841 acgccttatc cggcctacaa aagcatgcag attcaatata ttgcagaatt aacgtaggcc
    12901 tgataagacg cgccagcgtc gcatcaggcg ctgaatgccg gattgttacc cggcatttgt
    12961 catttagcgg tgatgtttgc ctggaccatg accgccgtga tgatcatgag gagctttctt
    13021 atggtggcgc ggcggtggcg gcggtccgtg tgggtgccag cgattgcctc gccattcata
    13081 atgttgtttc caccagccgt ggtcgcgcca gtgacctcca tcccagtaat agccacgatt
    13141 atcacgatcg cctatctgta attttactga cgggactaac gtaatttccg cagcctgtgc
    13201 tgccatggga gcgaccagaa ccagggaaag tgcgagtacg atagattgca tctttttcac
    13261 aggttactcc ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagaa
    13321 cgacggttgt tattctccgc aatcctaatc tctaagttac cgcatttatt gataatttct
    13381 cctttttccc tgtcgttttt ctccataatt tcttcataaa aaagccgggc tgcataaaag
    13441 caaacccggc ctgattgaga taatgaatag attttactga tgattcatca tcaatttacg
    13501 caacgcagaa aaatcggtgg gcagattatg tgaaagcaag ggtaaatcag cacgttctgc
    13561 cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt
    13621 cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggattcaact ggtcacgcag
    13681 cgcacgatac gctacggcag cgtgcggctc cgaggtgtag cccagttctt ttaactcacg
    13741 cattgtctgt tgcgtggttt catcatccac ggctgcataa cccagctctt tcagttgcca
    13801 gattttgcgg cggaacaact cttccacacg cggccagttg ttcggctgac tcacgtccat
    13861 cgcgttggat aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg
    13921 tggcacggta tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca gaccgaatga
    13981 cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agacaaccag
    14041 ctggttgcgc gcctcctgcg gcagctgcgc aacagcttca aagtagtagc aaatctgcgc
    14101 cagcaaacgg ctgatgttaa tcgagttagc cgagtttaac cccagcgcca ctttcagttc
    14161 ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc
    14221 aacagtttcg atattgccgc ccaacgtaca gaacagtttt tcttgcagtg gactgatttt
    14281 gcctcgtgga tagaggataa ccactttcac attcggcaaa ccgtagaaag catgagccac
    14341 tgcagctccg gtatcaccgg aggtcgcggt cagaatggtc actggcttat catccgcaat
    14401 atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc
    14461 gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaaggc
    14521 aaacgccgcg cgcacgcgct cttccaggat ttcctgtggg atttcatcac caataaacgc
    14581 cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt
    14641 caggctgaat tccggcaggt cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg
    14701 ggttacggct tgcgcaaagc tgacctgctc gttgtgatct ttcagattgt agagtttcat
    14761 ttagttttcc agtactcgtg cgcccgccgt atccagccgg caaatatgaa caaaaccttc
    14821 ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt
    14881 gtcacataga gcgaacaagg tcgggccgga gccggagata ccgctcgcta ccgcgccgat
    14941 ttccgcgacc gcctgccgcg cctgccggaa gccaggcagt aaccgttcac ggtagggttc
    15001 agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg
    15061 aatgaagcca gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg
    15121 taaaatagcc cgggcttctg ccgtcgagac tttaattccc ggatacgcca gcacccacag
    15181 ccactcatca aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg
    15241 cataccacca agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc
    15301 ttccagctcg cccatcaaag ccagcaaacg agtgtcatta agcggcttgc cgcaatgttc
    15361 attcatcgcc atcagcgccg cgaccaccga acaggcgctg gagcctaagc ccgaaccgat
    15421 cggcatattc ttttccagag tcatcgccac tggaatttgc ttgccaagct cctggcaaaa
    15481 acgctcccag cactgataaa cgatattttc ccgtggttct gacggcagct tatcggcaaa
    15541 gcgtccgagt ttgttgagac tgaatgtctc tgccgcctca accgtgacta catctccgag
    15601 caatgcacca tcaacgggtg ccaccgccgc cccgagcaca tcaaacccga cgctcatatt
    15661 ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg
    15721 tacgtagcag atcggcaaag acaccggcag cggtaacgtc attgcccgca ccatatccgc
    15781 gcagcaccaa cggcagcggc tgataatagt ggctataaaa ggccagggcg ttttcgccat
    15841 ttttcacttt gaacagcgga tcattcccat ccacttcggc aatcttcacg cggcagacac
    15901 catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg gccttcgcca
    15961 cgcgcgcggc aaagagatcg tcgagctgtg acagattcgc cataaaagcg gcaacatcac
    16021 cctcggcgtt aaactctgcg ggcagcacag gttcaatttc aatatccgcc agctccagtt
    16081 cacgtcccgt ttcacgagcg agaatcaata gtttacgcgc cacatccata ccagaaagat
    16141 catctcgcgg gtccggttcg gtataaccca tttcccgcgc cagcgtggtc gcctcggaga
    16201 aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa agaatgccgg
    16261 agaacttcac caattcatca cctgcattga gcagattttg caggttctca ataaccggta
    16321 atccagcccc aacgttggtg tcatagagga atttacgccg cgatttttcc gccgcataac
    16381 gcaactgatg atagtaatcc atcgacgagg tgttggcctt tttgttcggc gtgacaacgt
    16441 ggaaaccttc gcgcaggaag tcggcatatt gatccgccac tgcctggctg gaggtgcagt
    16501 caacaatgac cgggttcagc agatgatatt ctttcacgag gcgaattaag cgcccgagat
    16561 taaacggctc tttggcttgt gcaagttctt cctgccagtt ttccagatta aggccatgca
    16621 cactagtgag cagagccttc gagttggcaa cgccgcagac acgtaagtcg atatgtttat
    16681 tcttcagcca gctttgctga cgcttcagtt gctccagcag cgcaccgcca acgccaccga
    16741 cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc
    16801 gcacgccagt ggtcgcatca tcgttattta ccacgacaga gattgagcgt tcagaagatc
    16861 cctgagcaat ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg
    16921 agatcccacg caaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttccg
    16981 tcaccgccag cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg
    17041 cccgttcagc tcgcacacag tcgctttgtg gaacgcagaa actgatactg tattcggaag
    17101 atgattgcgt aatcagcacc acggaaatac gggcgcgtga cattgcagca aagacgcgcg
    17161 ccgccatgcc aaccatccct ttcatccccg ggccggaaac gctgaacatt gccatgttat
    17221 tcagattgga aatgcccttg accggtaatt cgtcttcatc acggctggca ccaatgagcg
    17281 tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctgg aactgggcga
    17341 tgggggtaat ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt
    17401 cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat
    17461 agaccccgtc aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag gcagccagca
    17521 ccgccgcgga gtagtcggaa ccgttacgtc caagcaccac cagttcgcct ttttcattac
    17581 cggcggtgaa acctgccatc aacaccatgt gatcagccgg aatgcggctt gccgcaatac
    17641 ggcgggtgga ctcagcaata tcgacggtag attcgaggta atgccccact gccagcagtt
    17701 tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg ccggccataa
    17761 tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttgatgcta tccgggcact
    17821 gccccaacaa actaatgcca tgcaggacat gttttatttg ggcaaattcc tgatcgacga
    17881 aagttttcaa ttgcgccagc gggaaccccg gctgggcggc ggcgagtccc gtcaaaagtt
    17941 cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatgtttt
    18001 tttcaatcat cgccaccagg tggttggtga ttttggcggg ggcagagagg acggtggcca
    18061 cctgcccctg cctggcattg ctttccagaa tatcggcaac acgcagaaaa cgttctgcat
    18121 ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg
    18181 tcgaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca
    18241 gcccgcaccg ttacctgtgg taatggtgat ggtggtggta atggtggtgc taatgcgttt
    18301 catggatgtt gtgtactctg taatttttat ctgtctgtgc gctatgccta tattggttaa
    18361 agtatttagt gacctaagtc aataaaattt taatttactc acggcaggta accagttcag
    18421 aagctgctat cagacactct ttttttaatc cacacagaga catattgccc gttgcagtca
    18481 gaatgaaaag ctgaaaaata cttactaagg cgttttttat ttggtgatat ttttttcaat
    18541 atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg
    18601 ctgtaaccag tcgaccagtt ttatgtcatc tgccactgcc agagtcgtca gcaatgccat
    18661 gactcgttcg cgtaaagctt gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg
    18721 ttgttgtatt aatgttgcta attgatagca atagaccatc accgcctgcc ccagattgag
    18781 cgaaggataa tccgccacca tcggcacacc agtaagcacg tcagccaacg ctaactcttc
    18841 gttagtcagc ccggaatctt cgcgaccaaa caccagcgcg gcatggctca tccatgaaga
    18901 tttttcctct aacagcggca ccagttctac tggcgtggcg tagtaatgat atttcgcccg
    18961 actacgcgca gtggtggcga cagtgaaatc gacatcgtgt aacgattcag ccagtgtcgg
    19021 gaaaacttta atattatcaa taatatcacc agatccatgt gcgacccagc gggtggctgg
    19081 ctccaggtgt gcctgactat cgacaatccg cagttcgcta aaacccatcg ttttcattgc
    19141 ccgcgccgct gccccaatat tttctgctct ggcgggtgcg accaggataa tcgttatacg
    19201 catattgcca ctcttcttga ccaaataacc gcgaacaggg tgatcactgt caacttatta
    19261 cgcggtgcga atttacaaat tcttaacgta agccgcagaa aaagcccttt acttagctta
    19321 aaaaaggcta aactattttc tgactgcact aacggtttaa ttgttaaaaa atgctacata
    19381 tccttctgtt tacttaggat aattttataa aaaataaatc tcgacaattg gattcaccac
    19441 gtttattagt tgtatgatgc aactagttgg attattaaaa taatgtgacg aaagctagca
    19501 tttagatacg atgatttcat caaactgtta acgtgctaca attgaacttg atatatgtca
    19561 acgaagcgta gttttattgg gtgtccggcc cctcttagct tgttatgttg ctgttaaaat
    19621 ggttaggatg acagccgttt ttgacactgt cgggtcctga gggaaagtac ccacgaccaa
    19681 gctaatgatg ttgttgacgt tgatggaaag tgcatcaaga acgcaattac gtactttagt
    19741 catgttacgc cgatcatgtt aatttgcagc atgcatcagg caggtcaggg acttttgtac
    19801 ttcctgtttc gatttagttg gcaatttagg tagcaaacat gcagaccccg cacattctta
    19861 tcgttgaaga cgagttggta acacgcaaca cgttgaaaag tattttcgaa gcggaaggct
    19921 atgatgtttt cgaagcgaca gatggcgcgg aaatgcatca gatcctctct gaatatgaca
    19981 tcaacctggt gatcatggat atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg
    20041 aactgcgcga gcaggcgaat gttgcgttga tgttcctgac tggccgtgac aacgaagtcg
    20101 ataaaattct cggcctcgaa atcggtgcag atgactacat caccaaaccg ttcaacccgc
    20161 gtgaactgac gattcgtgca cgcaacctgc tgtcccgtac catgaatctg ggtactgtca
    20221 gcgaagaacg tcgtagcgtt gaaagctaca agttcaatgg ttgggaactg gacatcaaca
    20281 gccgttcgtt gatcggccct gatggcgagc agtacaagct gccgcgcagc gagttccgcg
    20341 ccatgcttca cttctgtgaa aacccaggca aaattcagtc tcgtgctgaa ctgctgaaga
    20401 aaatgaccgg ccgtgagctg aaaccgcacg accgtactgt agacgtgacg atccgccgta
    20461 ttcgtaaaca tttcgaatct acgccggata cgccggaaat catcgccacc attcacggtg
    20521 aaggttatcg cttctgcggt gatctggaag attaatcggc tttaccaccg tcaaaaaaac
    20581 ggcgcttttt agcgccgttt ttatttttca accttatttc cagatacgta attcatcgtc
    20641 cgttgtaact tctttactgg ctttcatttt cggcagtgaa aacgcatacc agtctatatt
    20701 acgagtcaca aacatcatgc cggccagcgc caccaccagc acactggttc ccaacaacag
    20761 cgcgctatcg gcagagttga gcagtcccca catcactcca tccagcaaca acagcgcgag
    20821 ggtaaacaac atgctattgc gccagccttt caataccgcc tgcaaataaa taccgttcat
    20881 taacgcccca atcagactgg cgattatcca tgccacggta aaaccgatat gttcagaaag
    20941 cgccagcaag agcaaataaa acatcaccaa tgaaagcccc accagcaaat attgcattgg
    21001 gtgtaaacgt tgcgcggtga gcgtttcaaa aacaaagaac gccataaaag tcagtgcaat
    21061 cagcagaatg gcgtacttag tcgcccggtc cgttaattgg tattgatcgg ctggcgtcgt
    21121 cactgcgacg ctaaacgcgg ggaagttttc ccagccggta tcattgcctg aagcaaaacg
    21181 ctcaccgaga ttattagcaa accagctgct ttgccattgc gcctgaaaac ctgactcgct
    21241 agcttcccgt ttggctggta gaaaatcacc taaaaaactg ggatgcggcc agttgctggt
    21301 taaggtcatt tcgctattac gcccggcagg caccacagaa agatcgccgg taccgcttaa
    21361 attcagtgcc atattcagct gcaggttctg cttccgccag tccccttcgg gtaaagggat
    21421 atgcacgccc tgcccgcctt gctctaaccc ggtaccgggt tcaatggtca gtgccgttcc
    21481 gttaacttca ggcgctttca ccacaccaat accacgcgca tccccgacgc taatcacaat
    21541 aaagggcttg cctaagatga tatttggcgc gttgagctcg ctaagacgcg aaacatcgaa
    21601 atcggctttt agcgttaaat cactgtgcca gacctgaccg gtataaatcc ctatcttgcg
    21661 ttcttccacg ttctgattgc catcaaccat caatgactca ggtaaccaaa aatggataaa
    21721 acttcgtttc cgctccacgg ttttatcctc ttcctgcacc gtataaagct cggtcacagg
    21781 aatagcgata agcggcccaa cgagtttttg cggcccgctg gtactttggc gaatcgcatc
    21841 ttccacatcg ctacggtaat cggcacgttc gacaatcact tgccgaatca gcattatcgg
    21901 aatcaacaac agcaatactg caccaaacaa ggtggtcatt ttccagaaca ggggggattt
    21961 caacatagtc ttctcctttg caatggcagc aggatacgaa ggctatgtgg gaagaatttg
    22021 aagctatgtg aagtgacggt gaagtcgtag cgaggccagc acgccacctt cctgcacgtt
    22081 gcgcagcgtg acttcgccgt taaacaaacg ggcgacctca ctgacgaacg ccaaccccag
    22141 accgctgctt ttttgcccat ttgcacgcgg caaagagtaa aagcgttcaa aaatacgtga
    22201 cagcgcgtag tcaggaatac cactaccggt atccagcacc ttaagcgtga cgtgttcctg
    22261 atccacttcg gcgcttagcg ttatgcaacc gctctcggga gtaaaatcga tggcgttatc
    22321 cagtaaattc cccagcgcct gatccagtaa cgccggttca gcagcaacat taacctcggt
    22381 gggcataaca tgcaaagtga tgtttttttc tgccaactgc acggtgcgcg cttcgctgac
    22441 gcggcgaaat aatgccgcca catcaacagc agtcagaacg acttcctgac gattctccag
    22501 tcttgcctgg cgtagtaacg tttccaccag cgcctgcatt cgcgcatttt gtgtcagaat
    22561 gttgtcagta aaacgagcca ctacttcagg cggcggacct tcgcgtaaaa tttccgccgc
    22621 gccacgaatc gccgccagtg ggctttttag ctcatgggtt aacgcataaa catactgctc
    22681 aatatagttt ttcccttcca gcttcacgcg catactttcc agcgcctgcg cgagtttacg
    22741 cagctcgcta ctaccgagat cgggaagagg aacgggctta ttgtcagtga cggaatcagc
    22801 atagcgagtg agccgggcaa tggagcggtt gatccaccaa accatgcctg cgccaatcac
    22861 cagtgcaatc cccaacaaaa tggcgctggc ccataaaatt cgccgctcgc tgcgtttaat
    22921 gaccggagcc atcgccgcgt tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc
    22981 gcccataatc ggcgcggcaa catacatcac cgaactttcg ggatcggcag gattttgcaa
    23041 cgtgctgcgc gcaccatact gaccacgcaa cgttagccag acgtcattcc agcgcgaata
    23101 atcctgtcca acggctttat ttgccgaatc gaacaatact ttgccatgcg catcggtcat
    23161 atagacatga tattcattgc gcactttgtt aatgccacca atattggcgc gaaacgggcg
    23221 atgttgtagc tgattaaacg cctgcgccag ttgcccatgc gttgggtccc cagagagcaa
    23281 atcgggacgc gccagctccg caagtaacgt tgccgtgtcg atcaacgtcc cctccgttgc
    23341 tcttcgcacg cccggtttaa cttctttgac aaaaatagcc agtacgaacc aggccgccac
    23401 cgccaccagt aaaaaatagc ccagcagcaa ccgcatgccg atacgcatta caagcccctc
    23461 aggctatatc ccatgccgcg atgagtatta atcggtgaaa gatcggggtt gatggcgcgc
    23521 agcttggcac gcagcgtttt aatgtggata tcgacggtgc gatcgtaggt gtcctgcgca
    23581 tcttcccaca cgctatccat tagttgctgg cgggaccaga cgcggcctgg cgacttgagt
    23641 aacgtcttca gcaataaaaa ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg
    23701 atctgcgccg cgggttcatt caattcaaaa tgaccaatac ggatgacggg agacggcgtc
    23761 gagaacttct tcacccgacg cagtaaggtg cgcaccctgg cgcacacttc gcggggtgaa
    23821 aacggtttag ccacatagtc gtcagcgcca atttccagcc caagcaggcg atcgacctct
    23881 tcacttcggg ccgtcaggaa cagtacaggt aacgccggat ggagcgccag taactggcgg
    23941 cacaattcaa agccactgat atcaggcaaa ccaacgtcga gaatcatgac gtcgggaacc
    24001 tgctggcgag ctttatccag caccggcaaa cctcgctcaa agacctcgac ggcaaaacct
    24061 tcctgctgca acatgtagac cagcgtgtcg gctatccctt gctcatcttc cactaaccag
    24121 accgtttccc gttgcataaa tcgcctctgt tattgccgcc acggcatgac aggaaccgcg
    24181 ctaatcgcgt ttttcggcga accttctaca actttgtcgg agtaagccag ataagcgagc
    24241 gcgttgcgtt tggcgtcata aaagcgcacg acctgtaacg acttaaagac cagggacgtg
    24301 cgttttttga atactacctc gccctgagct ttgccgtttt taatacgatc cgacagttca
    24361 atcggcccga cttgctgaca agaaatggcc gcatcggagg tatcttctgc caggcccaat
    24421 ccccctttaa taccaccggt tttcgcccgg ctcacataac aggtgacatt tttcacatcg
    24481 ggatcgtcaa aggcttccac aacaattttg tgatccgggc cgatcatttt aaatacggtg
    24541 tcgaccgaac caatctcttc tgcatgagcc aatggcccca gcattatcag gcttaaagac
    24601 aggatcaaat gcttgtattt catattgtta ccatttttat ctattacacg agcaattagt
    24661 tcgtcacggt aaataacata ttgattaaga acaaaatctc aattctttta tttccgtcaa
    24721 gccctaaaac atactctact aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta
    24781 ttatccaatt acctgatgtc aggtgctcgt tgttgaaagg atgaggatat tttatggatc
    24841 aggccggcat tattcgcgac cttttaatct ggctggaagg tcatctggat cagcccctgt
    24901 cgctcgacaa tgtagcggcg aaagcaggtt attccaagtg gcacttacag agaatgttta
    24961 aagatgtcac tggccatgct attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg
    25021 cggtcgcact acgcctgact gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg
    25081 actctcaaca gacatttacc cgcgcattca agaagcagtt tgcccagact cctgcacttt
    25141 accgccgttc tcctgaatgg agcgcctttg gtattcgccc gccgctacgt ctgggtgaat
    25201 tcacaatgcc agagcacaaa tttgtcaccc tggaagatac gccgctgatt ggtgttaccc
    25261 agagctactc ctgttcgctg gagcaaatct ctgatttccg ccatgaaatg cgttatcagt
    25321 tctggcacga ttttctcggc aacgcgccga ccattccgcc ggtgctctac ggcctgaatg
    25381 aaacgcgtcc gagtcaggat aaagacgacg aacaagaggt attctatacc accgcgttag
    25441 cccaggatca ggcagatggc tatgtactga cggggcatcc ggtgatgctg cagggcggcg
    25501 aatatgtgat gtttacctat gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg
    25561 tatacggaac gtgcatgcca atgctcaatc tgacgcgccg taaaggtcag gatattgagc
    25621 gatactaccc ggcagaagac gccaaagcgg gagatcgccc aattaatctg cgctgtgaac
    25681 tgctgattcc tatccgccgt taacgctgca gctcatctaa tgcaggggcg tccagatgcg
    25741 agatgtcccc tgcggtttca acgacccagc cagacgccag ccacaggctt tcctgataat
    25801 ccacgcgcga aatagaacag ttacgcagac gtaagcggcg ctctgcccat gctggtaatc
    25861 cgagaatcgt actcaccaga catcccagtg caataccatg gctcaccagc agcggtcggc
    25921 ttccctgcgg taagtcccgg caagattcca gtgccgcatt gacgcgatcg ctgagctcct
    25981 gcattgactc gccttcagga atacgcccgt caacggtgcc attgaccagc tgccgacgcc
    26041 agttctcttc ttcttcggtc agagaatcga tatgtctttt ttccagcaca cccatgttta
    26101 attcacgcag gcgagaatca aagatgatgt cacagccgca ggcctgggcg atgatttccg
    26161 ccgtacgccg ggtgcgtcct aaatcgctac tgataatatg cgtaatgcca agctctttgg
    26221 cacgggttgc cacctgcatc gcctgttgct cacctttggc ggtcagcggg ctgtcagact
    26281 ggccctgaat acgtcgctcg gcgttccact gcgtttcacc gtggcggact aggtatacct
    26341 gtaacatgct ttttttccgt atactgctgt aacgttaatt ttctgagtaa tgctgattat
    26401 gcaccaagtt gtctgtgcga ccaccaatcc cgctaaaatt caggccattc tgcaggcatt
    26461 tcacgagatc ttcggcgaag gatcctgcca tattgcatcc gttgccgtcg agagcggtgt
    26521 accggaacaa ccctttggca gtgaggaaac gcgcgctggc gcacgaaatc gggtagcaaa
    26581 tgcccgccgt ttacttccag aggctgattt ttgggtggcg attgaagctg gcattgatgg
    26641 cgacagcact ttcagttggg tggtgattga aaacgccagc cagcgcggcg aggcgcgttc
    26701 ggcaactttg ccgttacctg cggtgattct ggaaaaagtt cgtgaaggcg aggcgcttgg
    26761 tcccgtgagt cgcgttatac cggtattgat gagattggtc gtaaggaagg ggcgatcggc
    26821 gtgtttaccg ccgggaaact cactcgcgcc agcgtctatc accaggcggt gatcctggcg
    26881 ctgagtccgt ttcacaatgc cgtgtatcag gcattgtagg acggataagg cgttcacgcc
    26941 gcatccgaca ttcggtgcac gatgcctgat gcgacgctgc cgcgtcttat caggcctaca
    27001 aaatcaatcg cttttcagca acacctcttc cagccactga cgcagctcca cgggcgcggc
    27061 tttcaggctg ttagatccac gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa
    27121 ctcacgctga ctcatttcgc cacgcaacag ctcttcgaca atacgcacgc gagtccccaa
    27181 cgcttcgcgc tcatctggcg tcagcatcag gtttaacaac ggtaaatgga gatcgttttg
    27241 gtaggcattc ttaagcaggt cgacaaaacg taaccactcc tggtgacgct gttctgccat
    27301 cgctgctgaa tagggtgatt gttgggccat aatatgtcgc cattgttagc gggggaagca
    27361 aaatgactcc cccggttgta ctcgctaaag agtacgatag catatcataa acgtgcggat
    27421 cagtaacgac gtccccattc cgtggcgctc atcaacgtcg gtttatcccc catgaaatag
    27481 cggtagtaag catcataagc cagcacgttc ttcacataac cgcgcgtctc ggagaacgga
    27541 atactctcga caaatgccac agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc
    27601 acccgtccag gtccggcgtt ataagctgct gaggagaaaa tacggttatt gccaaactgc
    27661 tgataaacat attgcaggta actggtgcca atgttgatgt tcgtttccgg atccagcaat
    27721 tgcccagggc tgctataacc aggaatagag aacatcttca ccgtatgggt cgctgtacca
    27781 ggcataatct gcatcaggcc gctggcccct accggtgatt tcactttcgg attccaggcg
    27841 ctctcctgac gggcaatcgc catcgcatag ctttgcggga tctctttccc actggtgtaa
    27901 cgtttgaaaa gatcgttgta agccagcggg aatcgctctt ccagatgatc ccaaagcttc
    27961 ccggcgatcg ttgcctgaac gctaagatcc caccattggt tattgaaagc ataccgagcc
    28021 agttgagcct gctctgtttt tgacttgctc ttcaccagat tggcccactc gctacgcgcg
    28081 gtattatcga gattccagta catcaactcg cgcacgcgcg ccatctccgg cccctgagtc
    28141 agggtgctgt caacattctg cggcgcttta tcaatcttca gctcatactc ttcaccgatg
    28201 cgttgtgctg caaccatcgg gtagaaacca cgctgttgta tgagttgatg caaaatctct
    28261 ttcgcttcag cttcacgtcc gcgttccagc aataaatccg cctgccagta acgccattca
    28321 tctttctctt ttgcttccat cggcagacgc gccagccagg tattcaggcc gcgacgatcg
    28381 ccggtgccaa gcgccattcg cacacggcgt tcaatcaacg acgtggattg cgagcgcata
    28441 atggcgtcat cgcgccattt cgcctgctca tcggtgacat cattgcccat caaacgccag
    28501 gcgacgatat cgcgcagctc ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc
    28561 gacgggatca tcagccgtgc attttcggca tcctgccgcg ccacactggc aaacgccacc
    28621 gccgccattt gacgggtaaa atcggtcgcg ccggttgtac gcgcgaaggt cagtaccgta
    28681 ttagggttgt tcgccagtga aatgattgcc gaggcgatag tctggtaatc ggcaggcatc
    28741 tgccctgcca gcactgttac caggcctgtg ttacccgctt tcatcgccag acggatacgc
    28801 tctaaatacg ccagcggatc ttgtttacct gacgcacgcc agacgctaaa taacttgtca
    28861 caggcgttag gctggctctt gccggttagc cacagctctt tcgccccttg ccaggcttct
    28921 tcactctgcc cggtgttcca tttcgcatag tagtaattac attgcgcttc ggtagttccg
    28981 ggcttttccg ggctaaaggc taacaagcca cgccagtctt cacgccgcgc cagttcattg
    29041 acgaaacgag attgcagcgt gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta
    29101 gtgaccgtca ccgccggttg attcatcagg tcatcggtga tctggcggta ttccaggtag
    29161 ggataaagcg gataatcctt cagtccaggc atcatttgtt cgaccacatc catctgtcga
    29221 ttatcccagg cctgcttgat ttgcgcgtaa cggctacgct gctcatccag tgagtcggct
    29281 cgcgccacgc tgctgaccgt cagcagacag acaccggcag ccaacagccg ccaggtaact
    29341 tgtttggctt tttccacaag cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc
    29401 cgcgtaatag atttatgcta accagtcatt gccgtttacg ccacgttacg gacacttttt
    29461 tacttttact gcgaaggtga tcgaccaact gggattaggc ggggaaacag gctacacttc
    29521 gcctttgaaa actcatcatc acgataaaca tagaggcgaa gtccaacgtg gctcaattcg
    29581 tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca
    29641 tctctctgag tttcttccct ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta
    29701 agtccaccct gctgcgcatt atggcgggca ttgataaaga catcgaaggt gaagcgcgtc
    29761 cgcagccaga catcaagatt ggttatctgc cgcaggaacc gcagctgaac ccggaacaca
    29821 ccgtgcgtga gtccattgaa gaagcggttt cagaagtggt taacgccctg aaacgcctgg
    29881 atgaagtgta tgcgctgtac gccgatccgg atgccgattt tgacaagctg gccgctgaac
    29941 aaggccgtct ggaagagatc attcaggctc acgacggtca taacctgaac gtacagctgg
    30001 agcgtgcggc ggatgcgctg cgtctgccag attgggacgc gaaaatcgct aacctctccg
    30061 gtggtgagcg tcgtcgcgtg gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc
    30121 tgctcgacga accgaccaac cacctggatg ccgaatccgt ggcgtggctg gaacgcttcc
    30181 tgcacgactt cgaaggcacc gtggtggcga ttactcacga ccgttacttc ctcgataacg
    30241 ttgcaggctg gatcctcgaa ctggaccgcg gtgaaggaat tccgtgggaa ggtaactact
    30301 cctcctggct ggagcagaaa gatcagcgtc tggcgcagga agcttcacaa gaagcggcgc
    30361 gtcgtaagtc gatcgagaaa gagctggagt gggtacgtca gggaactaaa ggccgccagt
    30421 cgaaaggtaa agcacgtctg gcacgctttg aagagctgaa cagcaccgaa taccagaaac
    30481 gtaacgaaac caacgaactg tttattccac ctggaccgcg tctgggcgat aaagtgctgg
    30541 aagtcagcaa cctgcgtaaa tcctacggcg atcgcctgct gattgatgac ctgagcttct
    30601 cgatcccgaa aggggcgatc gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc
    30661 tgttccgtat gatctccggt caggaacagc cggacagcgg caccatcact ttgggtgaaa
    30721 cggtgaaact ggcatcggtt gatcagttcc gtgactcaat ggataacagc aaaaccgttt
    30781 gggaagaagt ttccggcggg ctggatatta tgaagatcgg caacaccgag atgccaagcc
    30841 gcgcctacgt tggccgcttt aactttaaag gggttgatca gggtaaacgt gttggcgaac
    30901 tttccggtgg tgagcgcggt cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca
    30961 tgctgctgct cgacgaacca accaacgacc tggatatcga aaccctgcgc gcgctggaaa
    31021 acgccctgct ggagttcccg ggctgtgcga tggttatctc gcacgaccgt tggttcctcg
    31081 accgtatcgc cacgcacatc ctggactacc aggatgaagg taaagttgag ttcttcgaag
    31141 gtaactttac tgagtacgaa gagtacaaaa aacgcacgct gggcgcagac gcgctggagc
    31201 cgaagcgtat caagtacaag cgtattgcga agtaatgcgt aaaatgccgg atgcggcgcg
    31261 aacgccttat ccggcctaca aaaccagcaa tttcaatacg ttgtaaaact gtaggcctga
    31321 taagacgcgg taagcgtcgc atcaggcatt gagcaccgaa tgccggatgc ggcgtgaaca
    31381 ccttttccgg tctacaacag ccttagcatc ctcaatttcg tggcgggtca cccgtgcagg
    31441 ttttttcaaa ccttgtttaa tctcgacagc ctcttccaga gaggttttca actcatcaaa
    31501 aaaactcatt ttatacctcg cctttgagca gtttcgtcac ggttatcctt gctcccccat
    31561 catctcccgc accagctcca cgcagcgcag gaaacggctg tcgtaatcgt cctcttcaac
    31621 ccgcacgaat tcgatattgt tctcttccag catctccacc agcaagttct ggaactcttt
    31681 gcgatccacc gaactgccga ggctgcgtaa accatccgcc acccacggcg tgttgttctc
    31741 cagcaggatc accagatcga aacggtattc atcaatcagc gcctgcacga acggatgctc
    31801 acgcccttcg tactttttgc agaacgcctg ggtagtgaca aaatcggtgt caataaacgc
    31861 cactttattg gcgtatttca ctgcaaaatc aatgtattgt gcgtgaccca acgcgatttt
    31921 atcgtagtcg gaatactgca atgcgatctc atcaccgccg aggtgtgaaa agacataatc
    31981 gcggccatat tcccacgcac tggtggtgtt gaagatattg gcaagtttgt ttaccagggt
    32041 ggatttaccg ctcgactcgc cgccaaggat cgccacggta cgcacaaaaa acggcttcac
    32101 ttcggtagga atatattccc agtagcggaa cgggttttcg cggatctgcg caccgctgat
    32161 actcataaag gtacgtttcg gatcgaccag caccgtctcg atccccagat gttccatata
    32221 ctgtggcgca tcggcttctt ccgaggtgta gatcaggtcc ggctggatcc ccttttcagc
    32281 cataaacttt ttgatgccgt tgctccacac atcccagcca tgcggatacg gctccatgcc
    32341 ctcttcgttg aaagcatgaa tacggatatt tttctgatat ttaaaggttt gcaatagcca
    32401 gcgcagacga tccggcaccg tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg
    32461 gtcgcgggta tcgtcaaaac ccataatgat atgcagctcg tcaacctggc tacaggcgcg
    32521 ctggataagg tagatatgtc cggtatgcag cgggtagaac ttaccgaata cgacaccaat
    32581 ggttttcttc tgccggggaa attcaagccc caaaaaacgg tgcaatgcct ccagcttttg
    32641 cgcgctgggg cttttaattt tggcattcag taactggctt aaataccctt tggtcatacc
    32701 gctggcatca gctacctgct gtagcgtgca gccctgttgc ttgatggcag ttttcaggta
    32761 atcaaatgac gacatatctc cctccgtatc tctcattata agtcgtcgaa cacgctaagc
    32821 gcgtcggaga gttttttaac gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg
    32881 ttagccgccg gaacaatcgc ccggcgaaaa ccgtgtttcg ccgcttcaga aatccgctcc
    32941 tggccactgg gcaccgggcg gatctcccct gccagcccga cttcaccaaa caccaccaga
    33001 tcctgtggca gcggtctgtc acgcaggctg gaaaccatcg ccagcagtaa cgctaagtcg
    33061 gcgctggttt cggttacctt cacgccgccg accacgttca caaacacatc ctgatcggcc
    33121 atttgcagac caccgtgacg gtgcaagaca gccagcagga ttgccagacg gttttgttcc
    33181 agccccactg ccacgcggcg cggattcgcc attatcgagt gatcgaccag cgcctgaatc
    33241 tccaccagca acggacgcgt tccttcccac accaccatca cggagctgcc ggaggtcact
    33301 tcatctccgc gacttaagaa aattgccgaa gggttgctga cttcacgcag cccctgttcg
    33361 gtcatcgcga agacgcccag ctcattcacc gcgccgaagc ggtttttatg gctgcgtaag
    33421 gtgcgaaaac gggagtcggc atcgccatcc agaagcaccg aacagtcgat acagtgttcc
    33481 agcactttcg ggccagccag cgaaccatct ttggttacgt gtccaaccat gacaatcgcc
    33541 acaccgcgcg ttttggcgaa acgcgtcaga taagccgccg tttcacgcac ctgcgccacg
    33601 ctaccaggcg atgattgtac atccgccata tgcatcacct ggatcgagtc aattaccatc
    33661 agcttcggtt gctcttcttc ggcaatcagg cagatctgct cgatgctggt ttccgacaac
    33721 atattgagat tgtcagtcgg caggccaagg cgatgagcgc gcattgccac ctgttgcagc
    33781 gactcttcgc cggtgacata cagcgttttc atctgctggg ccagtttgca cagcgtttgc
    33841 agcaacagcg tggatttccc cgcaccaggg ttaccgccaa tcagaatggc acttcctggc
    33901 accacgccgc ctcctagtac gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc
    33961 tcttcaaggc tgatatcgga gagtttctgg acttttgcca ccccggcgct accggcatag
    34021 ccgctgagac gctcgttacg cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg
    34081 gtgttccagg catgacaggc actgcactgc ccctgccagc gcggataatc ggccccgcat
    34141 tcattacaaa caaaggcgcg ttttggagct tttgccacgg tgtacctcgt taatgctgtg
    34201 ccgcccgcag gatgacgggc aggcaattac ttctgattca ggctgccaga gaggatgcag
    34261 aataccccca tcaggtcagc gtgacggatg gtgacttccg ccttttcatt cacttttggc
    34321 ttggcatggt aggcaatccc cagccctgct gctttgatca tcggcaggtc attagctcca
    34381 tcgccaatcg ccacggtctg cgccagcggg atttcatact cctgcgcgag gcgagtcaga
    34441 gttttcgctt tgtactgcgc gtctacgatg tcgccgatca cattgccggt aaatttaccg
    34501 tccatgatct ccagttcatt ggctaccacg gctgtcaggc gcagcttgtc gcgcaggtat
    34561 tcagcaaaga aagtaaagcc gccggaggca atcgccactt tccagcccag cgtttccagc
    34621 ttgagcacca gctgcgttaa gcctggcatc agcggcagat tttcacgcac ctgttgcaga
    34681 atattggcgt cagcgccttt cagcgtcgcc acgcggctgc gcaggctggc ggtaaaatcg
    34741 agttcgccgc gcatcgcccg ttcggttacc tccgccacca tctcgcccgt tccggccagc
    34801 ttggcaattt catcaataca ttcaatctgg atggcggtgg aatccatgtc catcaccagc
    34861 aaacccggcg tgcgcaggtg cgggattttc cccagcgggg cgacatccag ctgcgcttcg
    34921 tgggccaggc gtgtagcccg tgcggtgagt gaacctgcca gacgaatcac ctgataatct
    34981 tccacgcacc aggcggcaac aatcaccatc gccgcaccca gtttgctctg gtattgggtc
    35041 agacgctgtt tatccagccc acgaccatac agcagccagc cgctacgacc tgcgtggtaa
    35101 tccagtggca tcacttcatc accacttaat gaaagaggca gacccggcca taaagagaca
    35161 tcttcaggca ggtcgcacca ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa
    35221 gcagggaaaa taacgcatga ggctaccttg tatccattgc ttctggcaac attaagtctc
    35281 aaattttcaa agggtggaag atggctcgca caaaactgaa attccggcta catcgggcag
    35341 tgattgtcct gttctgtctt gccttgttag tggcactgat gcagggggcg tcatggttta
    35401 gtcaaaacca ccagcgacag cgtaatccac agctggaaga actggcccgc accctggcgc
    35461 gtcaggtgac gctgaacgtt gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca
    35521 ttcaggcgat cctcgatcag ttaacagatg aaagccgtat cctcgacgcg ggtgtgtatg
    35581 acgaacaagg cgatcttatc gcacgttctg gcgaaagcgt cgaagtgcgc gatcggctgg
    35641 cgctcgacgg taaaaaagca ggcggctatt ttaaccagca gattgtcgag ccaattgcgg
    35701 gtaaaaacgg accgctcggc tatctgcgcc tgacactcga cacccatacg ctcgccaccg
    35761 aagcccaaca ggtggataac accactaaca ttttacgcct gatgttgctg ctctcactgg
    35821 caatcggcgt agtgctgacc cgcacgctgc tacagggtaa acgcacccgc tggcagcaat
    35881 cgcccttcct gttaaccgcc agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag
    35941 agtgacccat tactacaaga aaggaaatcg ttatgtccac attacgcctg ctcatctctg
    36001 actcttacga cccgtggttt aacctggcgg tggaagagtg tatttttcgc caaatgcccg
    36061 ccacgcagcg cgttctgttt ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc
    36121 agaacccgtg gaaagagtgt aatacccggc ggatggaaga agataacgtc cgcctggcgc
    36181 ggcgcagtgg cggtggcggc gcggtgttcc acgatctcgg caatacctgc tttaccttta
    36241 tggctggcaa gccggagtac gataaaacta tctccacgtc gattgtgctc aatgcgctga
    36301 acgcgctcgg cgtcagcgcc gaagcgtccg gacgtaacga tctggtggtg aaaaccgtcg
    36361 aaggcgaccg caaagtctca ggctcggcct atcgcgaaac caaagatcgc ggcttccacc
    36421 acggcacctt gctactcaat gccgacctca gccgcctggc aaactatctc aatccggata
    36481 aaaagaaact ggcggcgaaa ggcattactt cagtgcgttc ccgcgtgacc aacctcaccg
    36541 agctgctgcc ggggctcacc atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc
    36601 ccattatggc gagcacgtgg aagcggaaat catctccccg gacaaaacgc cagacttgcc
    36661 aaacttcgcc gaaacctttg cccgtcagag tagctgagag tggaacttcg gtcaggctcc
    36721 ggcattctcg catctgctgg atgaacgctt tagctggggc ggcgtggaac tgcatttcga
    36781 cgttgaaaaa ggccatatca cccgcgccca ggtatttacc gacagcctca accccgcgcc
    36841 gctggaagcc ctcgccgggc gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca
    36901 agagtgcgaa gcgctgttgg ttgacttccc ggaccaggaa aaagagctac gggagttgtc
    36961 gacgtggata gcgggggcgg taaggtaact cgctgattaa aatatccaag gaaggaaata
    37021 tgatcgatct ggaaaaccag gaacgggaga ttatcaattt aatgttcagc cagcgtattt
    37081 cctggctagc cgccgtgcgt atcaggcata aattatcgct tgctgaagta agtaaaatgt
    37141 taggtatctc gattaattct ctaaaacaga tcgaaaagac ggaacggctt agctcaaaca
    37201 tcaaaagcaa aatggcagaa atttatggtt gtccaccaga gttgctgata tgcccttcct
    37261 ggatgacggc tgaacataaa tgaatagatt cgtgaataga tacttgaatg ttttttattt
    37321 tctatccata tgaaaaataa tagtaaatct ataaatcagt tgctattgat gaatagatac
    37381 taaaaagccg gagcagtccc ccactccggc ttaacacgac aatcgccttt cagcgaaaca
    37441 caattactct ttatcgccca gcagaacgga ttccagtgcg attttgatca tgtcgttgaa
    37501 ggtggtctga cgctcagcgg cagtggtctg ctcgtgagtg cggatgtggt cagacacggt
    37561 gcagatggtc agggctttcg cgccaaactc tgcagccacg ccgtagatac cagccgcttc
    37621 catttccacg ccgaggatgc cgtatttttc catcacgtcg aacatttcgc cgtccggaga
    37681 gtagaacagg tcagcggaga acaggttacc cacgcgagca tcaacgccca gtgctttagc
    37741 tgcatctact gcgttacgca ccatgtcaaa gtcagcgata gcggcaaagt catggtcttt
    37801 aaaacggatg cggttaactt tggaatcggt gcaggccccc ataccgataa cgacgtcgcg
    37861 cagttttacg tgcggcagaa ctgcgccaca ggaaccaacg cggataattt tcttcacgcc
    37921 gaaatcggtg atcagttctt tggtgtagat ggagcaggac gggataccca taccgtgacc
    37981 cattacggaa attttgcggc ctttgtaagt accagtgaag cccagcatac cgcgaacgtt
    38041 gttcacttca cgggcatctt caaggaaagt ttcagcaata tacttcgcac gcagcgggtc
    38101 gcctggcatc aaaactacgt cagcgaaatc gcccatttct gcattaatgt gtggggtagc
    38161 cattgtttta tcctttttgt gacataacaa aggcggagtc aacgctccgc ccaaatccat
    38221 cagaacatgg ctttgccata ttccatatca gaagtaccaa aatattttgc cagagtctgg
    38281 ccgatatccg cgaaggtttc acggtgaccc agtgagcccg gttttacttt cgggccatat
    38341 accagtaccg gaatgtgttc acgcgtgtgg tcagtaccgg tccaggtcgg atcgcaaccg
    38401 tggtcagcgg tgaggatcag gatgtcgtca tcgcgcagca gagacatcag ctccggcaga
    38461 cggcggtcga acagttccag acccgcagca taaccggcga cgtcgcggcg gtggccccag
    38521 gaagagtcga agtcaacgaa gttggtgaag acgatggtgt tatcacccgc ttctttcatc
    38581 tctttgatgg tggcgtcaaa cagcgcgtcc aagccagtcg ctttcacttt tttggtgata
    38641 ccgcagttgg cgtagatgtc tgcaatttta ccgacagaaa ccacctggcc gtgtttttca
    38701 tcaaccagtt tctgcagcac ggtcggtgct ggcggctcaa cagccaagtc gtgacggtta
    38761 ccggtacgct ggaagttacc agctttgtca ccgataaacg gacgagcgat aacacgaccg
    38821 atattgtagc cgccgttggt cagctcttca cgggcgattt cgcacagttc gtagagttta
    38881 tccagaccga aagtttcttc atggcaggca atctggaaca cggagtcagc ggaggtatag
    38941 aaaatcggct tgccggtttt catgtgctct tcgcccagtt gatccagaat gaccgtaccg
    39001 gaagagtggc agttaccgag gtaacccggc agattagcgc gttcgaccag tttatccagc
    39061 agctcttgcg ggaagctgtt ttcgtgatcg gagaaatatc cccactcaaa cagaaccggg
    39121 acaccggcga tttcccagtg accagacggg gtatctttac cggatgacat ttcgtgcgcc
    39181 catgcgtacg cgccgataac ttcagcgttg ccgtccattc ccgccggaat gaaaccggta
    39241 gaaccttcgt gtgctttcgc cagccccaga cgggtcagat ttggcagatt gagcggccct
    39301 ttacgaccat tatcagcttc gcctttggca caagcttctg cgatatgacc cagggtgtca
    39361 gccccgacgt caccaaagcg ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc
    39421 accataataa atgcacgttt catatgttct ccgtactcag tgcttcaaat ttaaatgatc
    39481 agatcagtat accgttattc gctgatacgg cgatagacag ttggtgtgct ttccggtgcg
    39541 ttatcggcaa gtttaattgc cgctttcacc gctttcgccg cttcctgcca gctgttttcg
    39601 tctttcgcga ggataaccgc cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc
    39661 atatcagtaa agccgacgct gtaatcgatg gtgtcagatg cctgacgacg accgccgccc
    39721 attgcaacta ctgccatccc cagcgcgcgg gtatccattt cactgacaaa accttcggta
    39781 tcagcataga ctgctttcgt cagcatcgct gtcggcagat acttcgcgta gttctcaacg
    39841 aagtcggtcg ggcctttttg tgccgctacc atacgaccaa agacttctgc cgctttaccg
    39901 ttgtccagca ccgcctgcaa tttcgcgcgc gcttcggcgt catctttcgc cagtttgccg
    39961 gagatcaaca tctccacgca cagcgccatc gtgacatcaa acagacgcgg gttacgatac
    40021 tcacccgtca ggaactgcac cgcttcacga acttcaaccg cgttacctgc actggaggcc
    40081 agtacctgat tcatgtcggt gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg
    40141 ccaacaatcg cttcggcaag ggcttcagag agttcgtagg tcggcataaa cgcgccgcta
    40201 cccactttca cgtccatcac cagcgcgtcc agaccttccg ccagtttctt cgccaggatc
    40261 gaggcagtga tcagcgggat ggagtccacg gttgcggtaa tatcacgggt cgcgtagaaa
    40321 cgtttatcag ccggagccag tgaactggtc tgaccgataa tcgccacgcc gacgtcttta
    40381 ataatttcgc ggaaacggtt gtcatccggg aaaatgtcga agccagggat ggattccagt
    40441 ttgtcgagcg taccgccagt atgaccgagg ccgcgaccgg agatcatcgg aatatagccg
    40501 ccgcaggctg cgaccatcgg ccccaacatc agcgaagtga catcgccgac gccgccggtc
    40561 gagtgtttat caacaatcgg gccattcaga tgcaggcttt tccagtcgag aacggttcct
    40621 gaatctcgca tcgccatggt cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa
    40681 atggtcatcg cgagggcggc aatctgccct tcggaaatag tgttgtcgcg aataccgttg
    40741 ataaagaaac gaatttcttc atcgctcagc gcatgaccat cacgtttttt acgaataatt
    40801 tcttgtgcga gaaacaaggt aacctcccga ggaaaagagt atgaggcggc ttttgccgga
    40861 tgcgtctacg ccttatccgg cctacgggta gatacgtctc gtaggcctga taagcgcagc
    40921 gcatcaggcg taaagcatct tacttagtag ctgctggcgc tcttaccgtc gccgtggccc
    40981 agcgctttca gcaggcttgc cagcaggctg gaagcaccaa agcggtagtg acgcgcatca
    41041 gcccagtcag caccgaacag ttcatctgcg atggcgagat atttctgcgc atcttccgca
    41101 gtacgcacgc cgcccgccgg tttgaaacca acggtttttt ctacgcccat atcacggatc
    41161 acttccatca tgatgcgagc gctttccggc gtcgcgttca cagccacttt accggtagag
    41221 gttttgatga agtccgcacc cgctttgatg gagatttcag acgctttacg gatcagcgct
    41281 tcgtctttca gttcgccggt ttcgatgatc actttcagca gtacattcgc tgccgcgcaa
    41341 gcctctttac aggctttcac caggtcaaaa ccaacctgct cgtcacccgc catcagcgcg
    41401 cggtacggga acaccacgtc aacttcatca gcaccgtagg cgattgccgc acgggtttct
    41461 gccagcgcga tttcgatgtc gtcgttaccg tgtgggaagt tggttaccgt agcgatacgg
    41521 atttccgggg tgccctgctc tttcagagtt ttgcgagcaa tcgggataaa acgaggatag
    41581 atacagatag cggcggtatt gccgaccgga gttttggcct gatgacacag agcaattact
    41641 ttctcgtcgg tgtcgtcgtc attcagggtg gtcaggtcca tcaatttcag tgcacgcagg
    41701 ctgcttgctt tcagatcagt catttcattc tcctggcttg tcgccaataa aattcacctt
    41761 gcgagtttgt tagtattcta acatctactc cgcaacacac ttcgatacac atcacaatta
    41821 aggaaatcta cttacaagtt tgcatcactg taatgcgatc tggttcaaat aattcacttt
    41881 caaatgaatg cgtcagtggt ggcaaacgca tcaggatcaa agtgaacatc acgaaacttc
    41941 ttacaatggc gcatcacctt cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa
    42001 tgcactgcaa caacgttgcc agcaaattgt caccagccca gtgcttagcc cggagcagaa
    42061 gcgccatttt ctggcactgg aagcagaaaa caatctgcct tacccacagc ttcctgccga
    42121 agcccgccgc gcgctggatg aaggtgtaat ctgcgatatg tttgaaggtc atgcgccgta
    42181 caaaccgcgc tatgtcttac ccgattacgc tcgttttctg gcgaacggtt ccgaatggct
    42241 ggagctggaa ggcgcgaaag atcttgatga cgcactctct ctgctgacca ttctttacca
    42301 ccacgtaccg tcggtcacat cgatgccggt ctacctgggg caactggatg cgttgttgca
    42361 accgtatgtt agaattctaa cacaaaacga gatcgatgtt cgaataaaac gtttctggcg
    42421 ttacctcgac agaaccctgc cagatgcctt tatgcacgcc aatatcggcc cgtctgattc
    42481 gcccattacc cgcgcgatct tacgtgcaga cgcgcaggtt tcaccgaacc tgacctttat
    42541 ctacgatcct gaaatcaccc ctgatgacct gctgctggaa gtggcgaaga acatctgtga
    42601 atgtagcaaa ccgcacatcg ccaacggtcc ggtgcatgat aaaattttca caaaaggggg
    42661 ctacgggatt gtgagctgtt acaactcact gccgctggcg ggtggtggca gcacgctggt
    42721 acgccttaac ctgaaagcca ttgccgagcg cagcgaatcg ctggatgact tctttacgcg
    42781 cactctaccg cactactgcc agcagcagat cgccatcatc gatgcgcggt gtgaattcct
    42841 ctatcaacaa tcacacttct ttgagaatag cttcctggtg aaagaagggc tgattaaccc
    42901 tgaacgtttt gtgccaatgt ttggcatgta tgggctggcg gaagcggtta acttgctgtg
    42961 tgagaaagaa gggattgccg cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta
    43021 tcgcatcagc gcgcaactgg cggagtttgt cgccaatacc cccgtgaaat atggctggca
    43081 aaaacgcgcc atgttacacg cacagtcggg gatcagttcc gatatcggca ccacgccggg
    43141 cgcgcgtttg ccgtatggcg atgagccaga tccgatcacc catctgcaaa ctgtcgcccc
    43201 gcatcatgct tattattatt ccggcatcag cgacattctg acgctcgacg aaaccatcaa
    43261 acgtaacccg caggcactgg tacagctttg cctcggtgcc tttaaagccg gaatgcgtga
    43321 atttaccgcc aatgtcagcg gtaacgatct ggttcgcgtt accggttata tggtgcgttt
    43381 gtcggattta gaaaaatatc gcgccgaagg ttcacgcacc aacaccacct ggctgggcga
    43441 agaagccgca cgcaacactc gtattctgga acgccagccg cgcgtgataa gccatgaaca
    43501 gcagatgcgc tttagtcagt aagattatcc ccttctcctg cgttgacggg ccaggcagtc
    43561 gtctggctct gtttttgcag ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga
    43621 cgatgggacg ttgcaatgac tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc
    43681 agattgttga cggcaaagtg gtgtggaacg ctgtggtctg cgagcaatgt gatacctgcc
    43741 tgaagatgtg tccgcaacat gccacaccaa tggcgcaatc catgagcgtg gacgaagtgc
    43801 ttagccatgt gcgcaaagcg gtgctgttta ttgaagggat aacggtaagc ggcggcgaag
    43861 ccacgaccca actgccgttt gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac
    43921 tgcgccatct cacctgtctg gtggacagta acggtatgtt gagcgaaacc ggctgggaaa
    43981 aattactccc ggtgtgtgac ggcgcaatgc tcgatctcaa agcatggggt agcgaatgtc
    44041 atcaacaatt aaccggacgc gataatcaac agattaagcg cagcatctat ttgctggcag
    44101 agcgcggcaa gctggcggaa ctgcgtttgc tgatgatccc aggccaggtg gattatttgc
    44161 aacacatcga agaactggcg gcgtttatca agggacttgg tgatgttccg gttcgcctga
    44221 acgcgtttca tgcccacggc gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg
    44281 aagacgttga gccgttggct gatgcattaa aggtgcgcgg ggtgagccgg ttgatatttc
    44341 cggcgctcta tttgtgacat gccggatacg gcctacggtt cggcacagac ttgtaggcct
    44401 gataagacgc gtcaagcgtc gcatcaggca ttgtgcgcca actgccggat gcggcgtgaa
    44461 cgccttatcc ggcctacggt tcggcacaaa cctgtaggca tgataagacg cgttaagcgt
    44521 cgcatcaggc attatgcgcc gactgccgga tgctgcctac gaagggcaaa ctacccacga
    44581 acgttaaaca ccgcatacgt gttattaagc aacacttcgg caatctcatc cgcgggttcc
    44641 gggcgtaact cgcaaagcac ggcaaacaca cgggcagcct gttccgggcg gttaggctgc
    44701 ccctgaaaac cgttgagcgg catatccggc gcatcggttt ccagcaacaa cgatgccagc
    44761 ggtaattttg cgatgacatc gcgggtttta ctggcgcgtg gataggtgat agtaccgcct
    44821 acgccgattt tgtagcccag ctgtacaaac cgttcagcct gttgctggct gccagaaaat
    44881 ccgtggacaa caccagcacg cggcaaatcg tggcgtttaa gatgcatcgc cagtttgtcg
    44941 tgcgtgcgcc gtgaatgcag gatcaccggc agatcgtagc gtttcgccag tttcagttgt
    45001 tcgtcgagta accactgctg cctctcaaat tgcggatcgt cgccgaagag atccagaccg
    45061 atctccccca ccgccaccac cttcgccgga cgcctttcca gcgcctgctg tagctgctca
    45121 agagacacat cgctatgttt ttccaacata ccaggatgca agcccaatac ggcatacagc
    45181 ggttgataat tttccgctaa cgcctgcaca cgggcaaaat tttccgcctc tgtcgccgga
    45241 acaatgatct tgcctacgcc cgcttgtgcc gcgcgttgca ggctggcctc ttcatcgcca
    45301 ataaaaggcg ggaaatcaaa atggcagtga gtgtcgataa aacggcaaat caagccagat
    45361 cctcattgtt aaatgtcggg tcgttcgcct gcggagcgtc gctcacttcg gcaaccagcg
    45421 tatcgttggc gactggcgca ggcggaatga caatcgattc cggcgtcacc actggcacca
    45481 gatggcgggt aagcggcgct ttttcagtta atagcttgcc aaccgtggcg aggaaataac
    45541 gaccgcataa acgcccaagt ttatagtctt cacgcagcgc cggaatccga ctgccaagcg
    45601 cgatactatg taatggcttc ggcggataaa tttcggatat ccgcagcttg cccggtggtt
    45661 tctcaataaa ttgctgaatg tcacgatagc tggtttcatg atgctgcacc agattgacca
    45721 gcggctgcag gctactgtca cccagccagc gttccatgcg tttgaaccac tgcggcgtgt
    45781 agtacatttg tgacggcaca gtgcggatga cgaccaacgt tttagcgccc tgccttgccg
    45841 cctctttaac cggaatcgca tcactgatcc cgccatccag gtagttaatg ccttccagcg
    45901 acactccgct acgataaaag ccaggtatcg cactggaggc gcgaatcaca tccagccagt
    45961 tttgtttggt tggtaaaaag taattcggcg cgtagtcatc ctgacgacag gcgcacatat
    46021 aaaacgattt gccgctgtca aacaaccgcg cggcggtatc catttgcaac ggcatctggc
    46081 ttgcggtggc ctccaccagc cagtcgagat cgataagatt tcctccacgg acaaagcgca
    46141 atggatcgaa aaattcgcgt tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg
    46201 gctgattgca gataaacgcc gagaggttct gcgccccggc agatgtgccg agataaagat
    46261 cgaaaggatt aaactgcgcg cgcataaact catccagcac gccagccgtg aaaattccac
    46321 gctgtccgcc gccttcgcac accagagcta ttcgtccagg ctggaacggc cttagcgaca
    46381 acggcgcaat attaccaagc gttacaggta ttcgctgccc cacttctgcc ttcctgtttt
    46441 tgttgtgaaa gaaagacaaa gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt
    46501 ccgctggact ggctttggat aaaattttct taatggtctg aaatctaggg tcgttttcgg
    46561 cccatgaaca aactcactag gaacagaata atcccgacga caaagacaat tttagctgcg
    46621 cctgcagcgg taccggccag accaccaaac ccaagtgcgg cggcgattaa cgcgataacc
    46681 agaaatatga tgccccaacg aaacataagc gtctccttta ccatagttaa tgtcaccgct
    46741 tgatatgagc gtgaaaatca ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc
    46801 cgacaaagtt cgggaggacg aattacttag ttttcagatc atttttaacg cttttcacac
    46861 catctaccgc tttggcgata ctttcagcac ggtcactttg tgcctgagaa tctacggtac
    46921 cggagagctg aaccacgcca tcggtggttt caactttcac tttacgtgaa gggacgatat
    46981 cgtccgccag cagtttggct ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct
    47041 tcaccgagcc ttctttagcg tcgcgaacgt gcagtttgtc gctgacagag gtcacgcctt
    47101 caacgccttt cgccactttc actgcctctt cggcctgggc ctgactttca acgaaaccgc
    47161 tcagggtcac gactttttga tcggttttga cagagatatc ggtgctcttg atgttgtcat
    47221 gatccaccag cgccgccttc actttcgcgg tgatggcgct gtcatccatg aaattaccga
    47281 ctttattcat agagctatcg actttttgcc ctgcgctttc attggtagtc tgcgcgttgt
    47341 tttccgcgta ggcagagccg gtcgcgacgg cagaggtcaa cattacagcc agcagagttt
    47401 tcgaaatctt cagtcttgtc atagtcatcg atttattcct gtatgtttgc tcgtaatttg
    47461 agcctagcaa cacgaggttg cattgctgaa tagggagaga cttcaccctc tacagaagtc
    47521 aatggtcgcc atcacaaaag cgatgggtga tgaataacga ccattacagc ctctgaatca
    47581 gttattaata tcggtagaat gacaatcgac ggctttagat actaatatct acgcattgaa
    47641 cggtatttaa cgccatcaga aatgtcatca ctttgttaaa tatagatcac aattttgaaa
    47701 ccgctcggga tatcagcgaa aacataagca aaagtgaatg ttttaagaac attccgtaag
    47761 cggctaataa ggaagggaaa ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa
    47821 ttaatgctcg cgggtctggt ggaactgaac gtccggataa cgttcctgtg ccaggcgcag
    47881 gttgaccatg ctggtagcga tgtaagcgag gttatcgccg ccatcaagcg ccagttggct
    47941 ttcgttctta cgcttgaact cttcgaattt cttcgcgtct gcacattcta cccagcgggc
    48001 agtggcaacg ttgactgact catacactgc ttcaacgttg tattcgctct tcaggcgcgc
    48061 taccaccaca tcaaactgca gcacaccaac cgcaccaacg atcagatcgt tgttggagat
    48121 tggacggaac acctgcaccg cgccctcttc ggaaagctgt accagccctt tgagcagctg
    48181 tttttgcttc agcggatctt tcaggcggat acgacggaac agttctggtg cgaagttcgg
    48241 aataccggtg aacttcatca tctcaccctg ggtaaaggtg tcgccgatct gaatggtgcc
    48301 gtggttgtgc aggccgagga tatcgcccgg atacgcttct tcaacgtgcg aacggtcacc
    48361 cgccataaag gtcagcgcgt cggagatcac cacatctttc gcagtgcgca cctggcgcag
    48421 tttcatgcct ttttcatatt taccggacac cacacgcata aacgccacgc ggtcgcggtg
    48481 tttcggatcc atgttggcct gaattttaaa tacgaagccg gtaaatttat cttcgctcgc
    48541 ttctacggta cgggtatcgg tctgacgcgg catcggcgca ggcgcccact ccaccaggcc
    48601 attcagcata tgatcaacgc cgaagttacc cagcgcagta ccgaagaata ccggagtgat
    48661 ttcgcccgca aggaacagct ctttgtcgaa ctcgttagac gcgcctttaa ccagttccag
    48721 ttcgtcacgc agctgctgtg ccagatcttc accaaccgca gcatcgagat ccgggttatt
    48781 cagcccttta acaatgcgga cttcctgaat ggtgtgccct ttaccgctct gatagagata
    48841 ggtttcatct ttataaaggt ggtaaacgcc tttaaacagc ttgccgcagc caattggcca
    48901 agtgatcggt gcgcagccaa ttttcagctc gttctcaact tcatcgagca gttccatcgg
    48961 gtcgcggata tcacggtcaa gtttgttcat aaaggtgagg atcggcgtgt cgcgcagacg
    49021 ggtaacttcc atcagcttac gggtacgatc ttcaacacct tttgcggcgt cgataaccat
    49081 caggcagcag tccaccgccg tcagggtacg ataggtatct tccgagaagt cttcgtgccc
    49141 cggggtgtcg agcaggttaa ccaggcaatc gtgatacgga aactgcatca cagacgtagt
    49201 aatggagatc ccacgctgct tttccatctc catccagtcc gacttagcgt gctggttgga
    49261 accacggcct tttactgtac cggcggtctg aatggcctgt ccgaacagca gcaccttctc
    49321 ggtgatggta gttttaccgg cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc
    49381 cacctctttc aaataaggag acaacgtcat aattctttct tcttgagtaa gcggcatcgc
    49441 gccgcgcttg ttgaaaacga aaaattgcgg ctattttacc catcaacggg ggggaggcaa
    49501 tcagtgttta cacaggagct gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc
    49561 gatgccttct ggttgctcgc gattgtgcgc attcagccag caggtcgcaa gcccggcgtt
    49621 gatgccaccg agaatatcgg actcggcagt gtcgccaacc atcagcacgc gtgaacggtc
    49681 aggattgccc gcctgttcca gcgcataatc gaaaattttc ttattcggtt tggcaacgcc
    49741 aacttcttcg gaaatcacca gcaaatcgaa gtaatcacgc aggcccgtcc gttccagacg
    49801 cacttgctgc aaggcgctaa aaccgttggt gatgatgccg attttagcgt tgctacgaat
    49861 ggcgttaagc agagaaaccg cgcccggcaa cggcgtacag atttccgcca tcgcattaat
    49921 aaaggcatcg ttaagcaagc ctgcttcaac tttcagccgt tcggcccagc tctcgaaacg
    49981 cccgtgctga agctgtaatg aagtgatcgc gccgttttga taatccaccc acagtggctt
    50041 gttaacggcc tgatagtcct gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa
    50101 catccgctgc aggccggtga atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa
    50161 aatccagtcc cacttcatta ttccaccttg tcttacatac tgattggcaa cgccatgatg
    50221 atggcgtctt cgcgaccgtc cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta
    50281 aagcctaaac tttcgtacag ggcaatggcg gcagcatttg aggcacggac ttccagccat
    50341 agtgtcgcca cgccgcgttt ttccagttcg tcgatcagat gttccagcag cgccctaccc
    50401 agcccctgac gctgatagtc gggatcgacc gcaatattga acaatgtagc ttcatccagc
    50461 accacttgcg taatcgcaaa cgccgccatt ttgccgtttt gcgttaactg aaagttgaga
    50521 taacgctcgc cctggttgct ggcaaaggtt ttttcactcc acggaaaggc gtgggcgcgt
    50581 tgttcaatgt ggtaagccgc tggtaaatca gtcgtttcga gggaagaaat cgtgttcata
    50641 tgtgcaaatt tgttgccata acgcggcgcg tgccgttggg tttgcccgta attcggtgag
    50701 cgccggtgat gccacctgag cgccttccaa tgatagcggt tcgtcagtac ccaaccgcca
    50761 gctgttgcag cgactgcctt gcggcagcat cgcgattttt tctggcgtca gttgcagcac
    50821 ttgatcgggg ctgacggtta atgcgcgcag aacatcgctc actaaaggat cagtcagggc
    50881 gggaagatcg tttgccacca tcaccagacg gacgtgtgcc gggctcgcaa tggcaatctc
    50941 cccctgcaac gcgccagggc gacgcagcga ccactgggta atgcccagtt gctgtaactg
    51001 ccagtctcgt cgggatgtca tagcgaatcg ctcctgttgt caggggcgca aatatagcaa
    51061 attcgtcgat accgcgccaa catatggcta taatcgccgc cagtatcaat tgaggagcat
    51121 tccatgtctg catttacccc ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa
    51181 caaagccgta ttctgtttgc cggagactta caggatgacc tgcccgcgcg tttagatacc
    51241 gcggccagcc gtgctcatac ccagcaattc caccattggc aggtgttaag ctgccagatg
    51301 ggggataacg cccgttttag tctggtcgcc acggcgaatg atgtcgcaga ttgcgatacg
    51361 ctgatttact actggccgaa gaacaaaccg gaagcccagt tccagttgat gaatttactt
    51421 tctctgctgc cagtggggac agatattttt gtcgttggcg agaaccgcag cggcgtgcgc
    51481 agcgctgagc agatgctggc agattatgcg ccgttgaata aagtcgacag cgctcgtcgc
    51541 tgtggcctct attttggtcg tctggaaaaa cagccagtat ttgatgcgga taaattctgg
    51601 ggcgaataca gcgtcgatgg cctgacggtc aaaacgctgc ctggcgtgtt tagccgcgac
    51661 ggtctggatg tcggtagcca gttgctgctc tcgacgttaa ctccgcacac gaaaggtaaa
    51721 gtgctggatg tcggctgtgg cgcgggtgta ctttcagttg cctttgcgcg ccattcgccg
    51781 aaaattcgtc tcaccttgtg cgatgtctct gccccggcgg tagaagccag ccgcgcaaca
    51841 cttgcagcca acggtgttga aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa
    51901 ggttgttttg atatgatcat ctccaacccg ccgttccacg atgggatgca aaccagcctg
    51961 gatgcggcgc aaacgctgat tcgtggcgcg gtgcgtcatc ttaatagcgg cggcgagctg
    52021 cgaattgtag cgaacgcctt cctgccttac ccggacgtgc tggatgagac atttggcttc
    52081 catgaagtta tcgcgcaaac cgggcgcttc aaggtatatc gcgccattat gacccgccag
    52141 gcgaagaaag gttgagtatc tgatatctgt cgccgcatac cgtgtcggat gcggcgtgaa
    52201 cgccttatcc gacctacaaa acctgcacgt tagcccttcg taggccagat aagacgcgcc
    52261 agcgtcgcat ctggcatctg cccccgcatg cctaccaaac ctgcacgcta gtttcctgat
    52321 ggacattttt ccagcaatta cacatcaggc gataattaac tattgacgaa aagctgaaaa
    52381 ccactagaat gcgcctccgt ggtagcaatt ctttttaaga attgatggta tgcgaaggtg
    52441 gcggaattgg tagacgcgct agcttcaggt gttagtgtcc ttacggacgt gggggttcaa
    52501 gtcccccccc tcgcaccaaa aatcacgttg atattgctcg cact
//
LOCUS       DAJTPG010000016        49242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTPG010000016 DAJTPG010000000
VERSION     DAJTPG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..859
                     /locus_tag="OO230_001008"
     CDS             <1..859
                     /locus_tag="OO230_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5690859.1"
                     /translation="AASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLACA
                     GPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTKGM
                     SDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVPFN
                     FYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLPD
                     IPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQF
                     GVDIGEAAQ"
     gene            856..1821
                     /gene="yggF"
                     /locus_tag="OO230_001009"
     CDS             856..1821
                     /gene="yggF"
                     /locus_tag="OO230_001009"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5690860.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            1843..2352
                     /gene="fumE"
                     /locus_tag="OO230_001010"
     CDS             1843..2352
                     /gene="fumE"
                     /locus_tag="OO230_001010"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5690861.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2349..3062
                     /locus_tag="OO230_001011"
     CDS             2349..3062
                     /locus_tag="OO230_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5690862.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3034..3711)
                     /locus_tag="OO230_001012"
     CDS             complement(3034..3711)
                     /locus_tag="OO230_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5690863.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3705..4382)
                     /locus_tag="OO230_001013"
     CDS             complement(3705..4382)
                     /locus_tag="OO230_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5690864.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4370..4753)
                     /locus_tag="OO230_001014"
     CDS             complement(4370..4753)
                     /locus_tag="OO230_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5690865.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4769..5077)
                     /locus_tag="OO230_001015"
     CDS             complement(4769..5077)
                     /locus_tag="OO230_001015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690866.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5078..5656)
                     /locus_tag="OO230_001016"
     CDS             complement(5078..5656)
                     /locus_tag="OO230_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5690867.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5679..6110)
                     /locus_tag="OO230_001017"
                     /pseudo
     CDS             complement(5679..6110)
                     /locus_tag="OO230_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6482..7501
                     /gene="epd"
                     /locus_tag="OO230_001018"
     CDS             6482..7501
                     /gene="epd"
                     /locus_tag="OO230_001018"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5690868.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7551..8714
                     /gene="pgk"
                     /locus_tag="OO230_001019"
     CDS             7551..8714
                     /gene="pgk"
                     /locus_tag="OO230_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5690869.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8929..10008
                     /gene="fbaA"
                     /locus_tag="OO230_001020"
     CDS             8929..10008
                     /gene="fbaA"
                     /locus_tag="OO230_001020"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5690870.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10199..11059
                     /gene="mscS"
                     /locus_tag="OO230_001021"
     CDS             10199..11059
                     /gene="mscS"
                     /locus_tag="OO230_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5690871.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11198..11833
                     /gene="argO"
                     /locus_tag="OO230_001022"
     CDS             11198..11833
                     /gene="argO"
                     /locus_tag="OO230_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5690872.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11926..12666
                     /locus_tag="OO230_001023"
     CDS             11926..12666
                     /locus_tag="OO230_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5690873.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12833..13783
                     /locus_tag="OO230_001024"
     CDS             12833..13783
                     /locus_tag="OO230_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5690874.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13725..15203)
                     /gene="scpC"
                     /locus_tag="OO230_001025"
                     /pseudo
     CDS             complement(13725..15203)
                     /gene="scpC"
                     /locus_tag="OO230_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15227..16012)
                     /gene="scpB"
                     /locus_tag="OO230_001026"
     CDS             complement(15227..16012)
                     /gene="scpB"
                     /locus_tag="OO230_001026"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5690875.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16023..17018)
                     /gene="meaB"
                     /locus_tag="OO230_001027"
     CDS             complement(16023..17018)
                     /gene="meaB"
                     /locus_tag="OO230_001027"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5690876.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17011..19155)
                     /gene="scpA"
                     /locus_tag="OO230_001028"
     CDS             complement(17011..19155)
                     /gene="scpA"
                     /locus_tag="OO230_001028"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5690877.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19359..20252)
                     /gene="argP"
                     /locus_tag="OO230_001029"
     CDS             complement(19359..20252)
                     /gene="argP"
                     /locus_tag="OO230_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5690878.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20635..21294
                     /gene="rpiA"
                     /locus_tag="OO230_001030"
     CDS             20635..21294
                     /gene="rpiA"
                     /locus_tag="OO230_001030"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5690879.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21550..22782
                     /gene="serA"
                     /locus_tag="OO230_001031"
     CDS             21550..22782
                     /gene="serA"
                     /locus_tag="OO230_001031"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5690880.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23011..23070
                     /gene="ibsC"
                     /locus_tag="OO230_001032"
     CDS             23011..23070
                     /gene="ibsC"
                     /locus_tag="OO230_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5690881.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23403..23462
                     /locus_tag="OO230_001033"
     CDS             23403..23462
                     /locus_tag="OO230_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5690882.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23563..24165)
                     /locus_tag="OO230_001034"
     CDS             complement(23563..24165)
                     /locus_tag="OO230_001034"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5690883.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24186..24369)
                     /gene="ssrS"
                     /locus_tag="OO230_001035"
     ncRNA           complement(24186..24369)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO230_001035"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24411..24740)
                     /gene="zapA"
                     /locus_tag="OO230_001036"
     CDS             complement(24411..24740)
                     /gene="zapA"
                     /locus_tag="OO230_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5690884.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24908..25486
                     /gene="ygfB"
                     /locus_tag="OO230_001037"
     CDS             24908..25486
                     /gene="ygfB"
                     /locus_tag="OO230_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5690885.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25512..26837
                     /gene="pepP"
                     /locus_tag="OO230_001038"
     CDS             25512..26837
                     /gene="pepP"
                     /locus_tag="OO230_001038"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5690886.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            26834..28012
                     /gene="ubiH"
                     /locus_tag="OO230_001039"
                     /gene_synonym="visB"
     CDS             26834..28012
                     /gene="ubiH"
                     /locus_tag="OO230_001039"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5690887.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28035..29237
                     /gene="ubiI"
                     /locus_tag="OO230_001040"
                     /gene_synonym="visC"
     CDS             28035..29237
                     /gene="ubiI"
                     /locus_tag="OO230_001040"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5690888.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29311..29394)
                     /locus_tag="OO230_001041"
     CDS             complement(29311..29394)
                     /locus_tag="OO230_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690889.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29685..30779
                     /gene="gcvT"
                     /locus_tag="OO230_001042"
     CDS             29685..30779
                     /gene="gcvT"
                     /locus_tag="OO230_001042"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5690890.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            30803..31192
                     /gene="gcvH"
                     /locus_tag="OO230_001043"
     CDS             30803..31192
                     /gene="gcvH"
                     /locus_tag="OO230_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5690891.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31311..34184
                     /gene="gcvP"
                     /locus_tag="OO230_001044"
     CDS             31311..34184
                     /gene="gcvP"
                     /locus_tag="OO230_001044"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5690892.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34451..35194
                     /locus_tag="OO230_001045"
     CDS             34451..35194
                     /locus_tag="OO230_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5690893.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35251..36684)
                     /gene="bglA"
                     /locus_tag="OO230_001046"
     CDS             complement(35251..36684)
                     /gene="bglA"
                     /locus_tag="OO230_001046"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5690894.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            36729..37039
                     /locus_tag="OO230_001047"
                     /pseudo
     CDS             36729..37039
                     /locus_tag="OO230_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37203..37862
                     /locus_tag="OO230_001048"
     CDS             37203..37862
                     /locus_tag="OO230_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5690895.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38058..39038)
                     /gene="ygfZ"
                     /locus_tag="OO230_001049"
     CDS             complement(38058..39038)
                     /gene="ygfZ"
                     /locus_tag="OO230_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5690896.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39281..39547
                     /gene="sdhE"
                     /locus_tag="OO230_001050"
     CDS             39281..39547
                     /gene="sdhE"
                     /locus_tag="OO230_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5690897.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39528..39935
                     /locus_tag="OO230_001051"
     CDS             39528..39935
                     /locus_tag="OO230_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5690898.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(39975..40496)
                     /gene="fldB"
                     /locus_tag="OO230_001052"
     CDS             complement(39975..40496)
                     /gene="fldB"
                     /locus_tag="OO230_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5690899.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40608..41504
                     /gene="xerD"
                     /locus_tag="OO230_001053"
     CDS             40608..41504
                     /gene="xerD"
                     /locus_tag="OO230_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5690900.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41529..42239
                     /gene="dsbC"
                     /locus_tag="OO230_001054"
     CDS             41529..42239
                     /gene="dsbC"
                     /locus_tag="OO230_001054"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5690901.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42245..43978
                     /gene="recJ"
                     /locus_tag="OO230_001055"
     CDS             42245..43978
                     /gene="recJ"
                     /locus_tag="OO230_001055"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5690902.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44069..45167
                     /gene="prfB"
                     /locus_tag="OO230_001056"
     CDS             join(44069..44143,44145..45167)
                     /gene="prfB"
                     /locus_tag="OO230_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5690903.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45177..46694
                     /gene="lysS"
                     /locus_tag="OO230_001057"
     CDS             45177..46694
                     /gene="lysS"
                     /locus_tag="OO230_001057"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5690904.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46737..47285)
                     /gene="idi"
                     /locus_tag="OO230_001058"
     CDS             complement(46737..47285)
                     /gene="idi"
                     /locus_tag="OO230_001058"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5690905.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47340..47411
                     /locus_tag="OO230_001059"
     CDS             47340..47411
                     /locus_tag="OO230_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690906.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47408..47533)
                     /locus_tag="OO230_001060"
     CDS             complement(47408..47533)
                     /locus_tag="OO230_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690907.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47535..48983)
                     /gene="uacT"
                     /locus_tag="OO230_001061"
     CDS             complement(47535..48983)
                     /gene="uacT"
                     /locus_tag="OO230_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5690908.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11877 a        12343 c        13266 g        11756 t
ORIGIN      
        1 tgcggcgtcg ctggcagagc ctatgtgctg cattattggt gcttatcatg ccaattatca
       61 caccacgcaa tatgtttatg agcatcgcat gggcgtcaaa cctggcggca atattgcact
      121 gctggcgtgt gcgggtccga tgggcattgg cgctatcgat tacgccatta acggtggcat
      181 acaaccgtca cgggtggtgg tggtcgatat cgacgacaaa cgcctggcgc aggtgcagaa
      241 gctgctgccg gtggatctgg cggccagtaa aggcattgag ctggtgtatg tgaataccaa
      301 agggatgagc gatcctatcc agatgctgcg ggcgctgaca ggagatgtcg ggttcgatga
      361 catttttgtt tatgcggcgg tgcctgctgt cgttgagatg gctgacgaat tactggcgga
      421 agatggctgt ctgaacttct ttgccgggct gacggataaa aacttcaaag tgccgtttaa
      481 tttctacaac gtccattaca acagcacgca cgtagtcggt acatccggcg gttcaacgga
      541 cgacatgaaa gaggcgattg cccttagcgc cacaggacag ttacagccgt cgtttatggt
      601 gacccatatt ggtggcctgg atgcggtgcc agaaaccgtg ctcaatctgc cggatatccc
      661 tggcggtaaa aaactcattt ataacggcgt gaccatgccg ctcactgcca ttgccgattt
      721 tgccgaaaaa ggcaaaaccg atccgctgtt taaagagttg gcgcggctgg ttgaggaaac
      781 gcacggcatc tggaatgaac aggccgagaa atatctgctg gcacaatttg gcgttgatat
      841 cggggaggcc gcgcaatgat gtccctggcg tggccattat ttcgcgttac ggaacaggca
      901 gcgttggctg cctggccgca aaccggatgt ggcgacaaaa ataaaattga tggcctggcg
      961 gtcaccgcga tgcgccaggc attaaacgac gtcgcttttc gtggacgagt ggttatcggc
     1021 gaaggagaaa ttgaccatgc gccgatgctg tggattggcg aagaggtggg caaaggcgat
     1081 gggccagaag tcgatatcgc ggttgatccc attgaaggca cgcggatggt ggcgatgggg
     1141 cagagcaatg cgctggcggt catggctttc gctcctcgag atagcctgtt acatgcgccc
     1201 gatatgtata tgaaaaagct ggttgttaat cgactggctg ccggggcgat tgatctgtct
     1261 ttgcctctgg cggacaactt gcgtaacgtg gcgcgggcgt taggtaaacc gctggacaag
     1321 ctgcgcatgg ttacgctgga taaaccgcgt ctgagtgccg cgattgaaga agcaacacag
     1381 ctgggcgtga aggtttttgc cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc
     1441 tggcaggata atccttacga tgtgatgtac accatcggcg gtgcgccaga aggcgtgatt
     1501 tctgcctgtg ccgttaaagc gttaggcggg gatatgcagg ccgagctgat tgacttttgt
     1561 caggctaaag gggattacac ggaaaatcgg cagattgcag agcaggagcg caagcgttgt
     1621 aaggcaatgg gcgttgacgt taaccgtgtg tactcgctcg atgaactggt gagggggaac
     1681 gatatcctct ttagcgctac gggcgtgacg ggcggcgagt tggtgaacgg tatccaacag
     1741 acggcgaatg gggtgcggac gcagacatta ctgatcggcg gcgcggacca aacgtgtaat
     1801 ataatagact ccctgcattg atggtgatct atcggaagac caatggcgac gctgacagaa
     1861 gatgatgtgc ttgagcaact ggatgcacag gacaatttat tttcatttat gaaaactgcg
     1921 cattctattt tgctccaggg gatacgccag tttctgccgt cgctgtttgt cgataacgat
     1981 gaagagatcg tcgaatatgc agtgaagccg ttactcgccc aaagcggccc gcttgacgat
     2041 attgatgttg cgctgcgttt gatttatgcg ctggggaaaa tggataaatg gctgtatgcc
     2101 gatatcacgc acttttccca gtactggcat tacctgaacg aacaggatga aacgcccgga
     2161 tttgccgatg atataacctg ggattttatc agcaatgtca acagtataac tcgcaatgcg
     2221 acgctctacg atgcgttaaa agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg
     2281 cgttttagcg gaatggtcaa aacggcgctg acgctggcag taacgaccac cttaaaggaa
     2341 ttaacgccgt gaaaattgaa ttaacggtga atgggctgaa cgtgcaagcg cagtaccatg
     2401 atgaagagat agaacgcgtg cataaactgt tgctgcgtat gctggcggcc ttacagacgg
     2461 taaatccgca gcggcgtacg gtggtttttc tttgcgctcc gccggggacg ggtaaatcta
     2521 ccctaaccac cttctgggaa tatctcgcgc aacaagaccc ggagctgccc gctattcagg
     2581 cgctcccgat ggatggtttt caccattaca atagctggct ggacgcgcat caattgcgcc
     2641 ccttcaaagg cgcaccagag acatttgacg ttgcgaaact ggcggaaaat ctgcgccagg
     2701 tggtggaagg ggattgtacg tggccgcagt acgatcgaca aaagcatgat cctgttgaag
     2761 atgcgttgca cgttaccgca ccactcgtca tcgtcgaagg aaactggttg ttgctggacg
     2821 acgagaagtg gtgccagctt gcgcaatttt gtgatttctc gatctttatc aacgcgccag
     2881 ctgctgcgtt gcgggagcgt ctggtggggc gcaagctggc gggaggggta tcgctggcag
     2941 atgcagaagc cttttatgac cgtaccgacg ggccgaatgt tcgccggggg ctggaagaga
     3001 gtctgcctgc aaatttgacc ttaatgatga ccgctacggg tgaatatcgt ctggtggatt
     3061 aacgttcctg attacaccat cttccagccg caccacacga gagaaatgac gccgggtaaa
     3121 tgcggcgtca tgactgattg ctaccacgct cgtgcctcgc tggcggcact tctccagcca
     3181 gctttcaaac acgctcaacc aattttcgtc aaaatcccga ctcggctcgt ccagcagtaa
     3241 tagtggcgga gagagtgctt ccaggcaggc gacggcgacc atccgtcgct gcgcattatg
     3301 cagatccaac gggtgcgagt tggcagtgtc tgctaactga caacattgca gggcagcatg
     3361 ggtacgttga gtgatttcat ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc
     3421 gtcagcgacg gtgctgtgaa aaagttggtt ttccgcttcc tgaaacagta cgccaacctt
     3481 tgccgcgcgc tggcggtttt tcaggttttt catcgcctgt tgttgcagca taaccgtacc
     3541 ggctgtgggc gtaagaagac ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt
     3601 gtcaccggtc agcgccaacc attcaccttg tttgagttgc agagaaatgt cgcaaagaca
     3661 atccgttgcc gcgccaggcc agcgataaga tatcttattt aacgttaaca tagtggctgg
     3721 agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga
     3781 cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac
     3841 agcatttcgc tggcctgcgg tgtcaggcgg ctaaaggctt catcaagaat taagagcttt
     3901 ggctgcatcg cgatggcgca ggcgatgatc acgcgctgtg tctctccacc ggagagtgtt
     3961 gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc atcaatccgc
     4021 gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac ttcttcttcc
     4081 acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag ctggatggtg
     4141 gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt ccccgtgcct
     4201 gttatctctc ttggcagaaa gtccggatac cagcccgcca tcagctgtgc cagcgtactt
     4261 ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata gtgaaagtca
     4321 taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc taacgtaacc
     4381 atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt
     4441 gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc
     4501 gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt gttagcggaa
     4561 taattaacgc gggcatagca cgtaaacgct gataccagcc ctcatccagc ggcacgccgc
     4621 gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca acgaccaata
     4681 acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac agagcgcgaa
     4741 taaaacattg cacggggacg tactgcattc acaactgtga ggttgaaaca atcgccagaa
     4801 tcagcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc gtgccgctta
     4861 gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc gagaacatca
     4921 gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg cagaaaaagg
     4981 tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg
     5041 ctaatgccca cagtgttaat gaggtaaatg gatgcattta gcgcacggca gccattgccg
     5101 gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca atcaccgctg
     5161 taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac tccaccaggt
     5221 tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg gtgacaccgc
     5281 cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta ataatcacgc
     5341 cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc caaccggaga
     5401 ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg gtcagcaatc
     5461 cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca agcagtaccg
     5521 cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg ctggccagtt
     5581 gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc tgactggaaa
     5641 agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg ggaaatatgc
     5701 aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta
     5761 ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg accgccaagc
     5821 atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg ttccagcgtg
     5881 ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc ttgcccggca
     5941 tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatctaggc ggcatgaagt
     6001 tgatgttgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc
     6061 agtgcgtaaa agcgtgcgga ggaagaatat gagtagggca atgtcatcat tggagtactc
     6121 cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc tgatataagt
     6181 cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt
     6241 ggaataaatt ccacaggggc ggggctgaaa acgtgacgta agtcagataa ttgctttctt
     6301 tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt
     6361 cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg
     6421 gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc
     6481 tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc
     6541 tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga
     6601 tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt ttgcatggga
     6661 agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga
     6721 acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac
     6781 cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg ccaaaaaagt
     6841 gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca
     6901 ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat
     6961 aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac
     7021 aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg
     7081 cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg ctggtatcac
     7141 acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat
     7201 aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt
     7261 caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga
     7321 attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac
     7381 ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg
     7441 gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta
     7501 agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa
     7561 ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg
     7621 taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca
     7681 tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta
     7741 ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac ctgaaagaca
     7801 aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag
     7861 gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa
     7921 ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg
     7981 ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag
     8041 gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa cctgctcgcc
     8101 cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt ctggactccc
     8161 tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg
     8221 cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac gaagctaaac
     8281 gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca accgagttct
     8341 ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac gagcagatcc
     8401 tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca
     8461 ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa
     8521 tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc
     8581 tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg
     8641 gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc
     8701 gcgctaagaa gtaaaaaatc acagggcagg gaaacctgca cttgtttcag cgcgctttta
     8761 gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga
     8821 atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc
     8881 aacttgaagt attatgagta taaggcccga cgatacagga caagagacat gtctaagatt
     8941 tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta
     9001 gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac
     9061 gccgtactgg aaactgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt
     9121 ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc
     9181 ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg
     9241 gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg
     9301 gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc tcacatgatc
     9361 gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgt
     9421 atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac
     9481 ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat
     9541 tacgcataca ccgagctgag caaaatcagc ccgcgtttca ccatcgctgc gtccttcggt
     9601 aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat cctgcgtgat
     9661 tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgaa cttcgtattc
     9721 cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg
     9781 aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac
     9841 aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg
     9901 aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct
     9961 cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaagt cattcctttc
    10021 tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaagg tgattcagat
    10081 gagaaatctg tgatctattt ggcaaaatta tgctttattg tttacccttg tcagactgcc
    10141 cgtcataagg cggcggagtg tatttctcca ttttgagtca gttgaaaagg aatattgaat
    10201 ggaagatttg aatgttgtcg atagcataaa cggcgcggga agctggctgg tagctaacca
    10261 ggcgctgctg ctaagttatg cagtaaacat cgtggcggca ctcgcgatca tcatcgttgg
    10321 tttgattatc gcgcggatga tttccaacgc ggtgaatcgc ctgatgatct cccgtaaaat
    10381 cgatgccact gttgctgatt ttctttctgc attagtccgt tacggtatta tcgcctttac
    10441 gctaatcgct gccctgggac gcgtgggtgt acaaacagcg tcagtcattg ctgtactcgg
    10501 tgccgcaggc ttagctgttg gtctggcttt gcaggggtca ctttctaacc tggccgctgg
    10561 cgtgttactt gtcatgttcc gcccgttccg tgccggagaa tatgttgacc tcggcggcgt
    10621 agccggtact gtgctgagtg tgcagatttt ctccaccacc atgcgtactg cagacggtaa
    10681 aattatcgtt attccgaacg gtaaaattat tgccggaaat attattaact tctcccgcga
    10741 gccagttcgc cgtaacgaat ttattattgg cgtggcgtat gattccgata tcgatcaggt
    10801 taagcagatc ctgaccaata ttatccagtc tgaagatcgc attttgaaag atcgcgaaat
    10861 gactgtgcgc ctgaacgaac ttggtgcatc gtcgattaat ttcgtggtcc gcgtctggag
    10921 caacagcggc gatctgcaaa acgtgtactg ggatgtgctg gagcgtatta aacgtgaatt
    10981 tgatgccgcc ggtatcagct tcccgtaccc gcaaatggat gtgaacttta agcgggtgaa
    11041 agaagacaaa gctgcgtaat caacgctgag gccagataat actctctggc ctctctctta
    11101 ttagttattc tgattgccaa ttaatattat caatttccgc taataacaat cccgcgatat
    11161 agtctctgca tcagatactt aattcggaat atccaacgtg ttttcttatt actttcaagg
    11221 tcttgctctt ggggcagcta tgattctgcc gctcggccca caaaatgctt ttgtgatgaa
    11281 tcagggcatt cgtcgtcagt accacattat gattgcctta ctttgcgcta tcagcgattt
    11341 ggtcctgatt tgcgcgggga tttttggtgg tagcgcgtta ttgatgcagt cgccgtggtt
    11401 gctagcgctg gtcacctggg gcggcgtagt cttcttgctc tggtatggtt ttggcgcttt
    11461 taaaacagcg atgagtagta atattgagtt agctagtgcc gaagtcctga agcaaggcag
    11521 atggaaaatt atcgccacca tgttggcagt gacctggctg aatccgcatg tttacctgga
    11581 tacttttgtt gtactgggca gccttggcgg gcagcttgac gtcgaaccaa aacgttggtt
    11641 tgcgctcggg acaattagcg cctctttcct gtggttcttt ggtctggcta ttctcgcagc
    11701 ctggctggca ccgcgcctgc gcacggcaaa atcacagcgc attatcaatc tggtagtggg
    11761 atgtgtcatg tggtttatag ccttgcagct ggcgagagac ggtattgctc atgcacaagc
    11821 cttgttcagt taggcgctat ctgatggaaa aataaaacag aggcgctaag cttgcctcca
    11881 gaggtcctga attttgggca agtcaattaa cacggagaga ctaacgtgaa gttcaaagtt
    11941 atcgccctgg cggcattaat gggtattagc gggatggcag cgcaggctaa cgaattgccg
    12001 gatggaccgc atattgtcac ctccggtacg gcaagtgtgg atgcggtgcc agacattgcc
    12061 actcttgcga ttgaagttaa cgtggccgcg aaggatgccg ctacagccaa gaaacaggca
    12121 gatgagcgcg tcgcacaata catttccttc cttgaactca atcagatcgc gaaaaaagat
    12181 atcagctcag cgaatttacg cacccagcca gattatgact atcaggatgg taaaagtatc
    12241 ctgaaaggct accgagctgt gagaacggtg gaagtcacgc tccgtcagtt agacaaactg
    12301 aattccttgc tggatggcgc gctgaaggcg ggtcttaacg aaattcgttc tgtgtcgctg
    12361 ggcgtggcgc agccggatgc ctataaagac aaagcgcgta aggcagcgat tgataacgcg
    12421 attcatcagg cgcaggaact ggcgaacggc tttcatcgta aactggggcc ggtatatagc
    12481 gtgcgctacc atgtttccaa ctatcagccc agcccaatgg tgcggatgat gaaagccgat
    12541 gccgcgccgg tgtctgccca ggaaacttac gagcaggccg ctattcagtt tgatgatcag
    12601 gtcgatgtgg tcttccagtt agaacctgtg gatcaacaac ccgctaaaac acctgcagca
    12661 caataatact tgtagcccga cgtattcgtc gggctaatgc ctttacccga tattgcctcc
    12721 tttttctgta attttctgta aaaccatgac gtaatcacct atctttgctc gcggtttcat
    12781 caaggatagg ctgactgaaa cggttaagcg gacaaggatg cggggagggg ggatggacat
    12841 ttttatttcg aaaaagatgc gcaatttcat tttattagcg caaaccaata atattgcccg
    12901 ggcggcagaa aaaatccata tgacggcttc gccatttggt aaaagcattg ccgcgctgga
    12961 agaacaaatt ggctatacgc tatttacccg caaagataat aatatcagcc tcaataaagc
    13021 cggtcaggaa ctgtatcaaa aactgttccc ggtttatcaa cgactttctg cgattgataa
    13081 tgaaatccat aactcagggc gtcgttcacg agaaattgtg attggcatag ataatactta
    13141 tccgacaatt attttcgatc agttgattag tctcggagat aagtatgaag gcgtgaccgc
    13201 ccagccagtt gagttcagcg aaaacggcgt tatcgataat ctttttgacc gtcagttaga
    13261 ttttattatc tccccacagc atgtatctgc acgagttcag gaactggaaa acctgactat
    13321 cagtgaatta ccaccgctgc gattgggctt tctcgtttct cgccgttatg aagagcgtca
    13381 agagcaagag ctgcttcagg agctgccctg gctacaaatg cgctttcaaa accgggctaa
    13441 cttcgaagca atgattgatg cgaatatgcg accttgtggg atcaatccca ccattattta
    13501 tcgtccctac agttttatgg ccaaaatcag cgccgtggag cgcggacatt ttctgacggt
    13561 gatcccgcat tttgcctggc gactggtgaa tccggcaacg ctgaaatatt ttgatgcgcc
    13621 gcacaggccg atgtatatgc aggaatatct ttattcaatc agaaatcatc gttataccgc
    13681 cacgatcttc agcatattgc tgaagatcgt gacgggacaa accattaacc cagcatcgag
    13741 ccggttgcaa ttaaattacg gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt
    13801 ccgccgggcg cattttccag atagcgatgc agataatccc gatacatagg atgtgcacaa
    13861 ttatcaatga tagtgcgggc gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct
    13921 tgttcggtga tgatcacttt gacgctgtgt tcgctgtgat caacatggct gcacattggc
    13981 acgacggttg agatcttgcc ttctttagca atcgacggag ccataaagat cgacagatac
    14041 gcgttgcgtt caaaatcacc gctgccgccg atgccgttca tcagatcgac cccggctacg
    14101 tgcgttgaat ttgcatgccc gtaaatatca aactccaggc cgacgttcag agcgatgacg
    14161 cccagacgac ggatgatttc cgggttattg gaaatctcct gcggacgcaa cacaatgcgg
    14221 ctggcaaagt aatccatatt gtcgtaaatc ttgcgcaggg aatcggccga gattgtcagg
    14281 ctggaggcgc tggccccgct gattttgccg gtttccagta aatgcaccac cgattcctgt
    14341 agcacttccg aatacatcat gaacggagga atttccgggt tttcccccag acgtgccatt
    14401 accgcattat tgatattgcc cacgccactt tgcagcggca gaaattccgg cggaatacgc
    14461 ccatgcgcca tttcctgcaa taagaacgtg accacgttat cggcaatctg ctggcacatg
    14521 ggattttgct tatccagcat attaccggcg tcgggcaagt tggtttccac gacggcgaca
    14581 atctttttcg gatcgatttg cacatagcgg gtaccgacgc gatccattgc atggaagatc
    14641 gacacgctat tgcgccgcgg tggcgcgcca ggaatcacaa tatctgccag ttctgcaacg
    14701 cgcggatcgt gatagtgatt aagttcaatg atcactttct tcgctcgcag cagctaggtc
    14761 ggcgcattac cgatcccgct ggttaaccag actcgaccat ccggtgccag tgccgatgct
    14821 tcaatgacgg caacatcaat gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg
    14881 ctcaaatgca ggtcaacgaa actcaccgcg ccctgattga tctttttacg taaactggac
    14941 gatgtttgat atggcgcacg ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg
    15001 gcggcgctga ttgacgcccc cgtcagcagg cgaatttgat acggcttttt ggcctcatgc
    15061 tgttcgttag ctctgcgggc aatcgcggcg ggtagggctt tcggcgaacc cgctggggta
    15121 aagccgctaa atgccaccat gtcgttatgc tggataattt ctgccgcttc atcggcggtc
    15181 atccttgtcc actgagtttc catttttact ccttcgttcg cagggattaa tgaccaacga
    15241 aattaggttt acgtttttcg aggaaagcgt tcatcccttc ctggtaatct tcgctgtcat
    15301 aaaccgcgcg gcgcatcccc tgaatacgtt caaattcatc ggagttcatg gtgtgtgctt
    15361 cgcccagtac acgcagctct tctttgataa cggcaatggc taacggcgct ttctcagaga
    15421 tgtggtgcgc catttgtaag gtgaaatctt ccagttcttc cacttccaca acatggttga
    15481 ggatgccgac agccagcgcg cgctgggcgg tgattggcga agcggtaaaa atcagctctt
    15541 tgacaatgtg gaagcccgcg tcgcgggtca ggttgtgaat gccgaccagg ttatacggga
    15601 cgccgaggtt tacaggcgtc attgagaagg ttgaggtact ggcggcgatg atcagatcgg
    15661 aactcatgat catttcaaat gcgccacccc aaacactacc ttccaccatc gaaatgatcg
    15721 gtttcgggaa tttttggatc atgcgggtga tttgacgcaa tggatcatca taggagagcg
    15781 gatcgcgacc gccagacggc agttcgtgaa tatcgtgacc tgcggagaag actttggatc
    15841 cacttggcgc ccgcaaaatg atacagcgaa tttccggccg gttgagatcg cttaacgcct
    15901 gcataagatc atcaataaag actttactta aggcattaag ttttcgtcca tagttaaact
    15961 caatgaccgc cactttgttg atagtggcaa cgttcacata ctgataagac ataaaaattc
    16021 ctttaatcaa aatattgcgt ctggataaat tcactgagct gccgcaggcc ggtgcgcggt
    16081 gagagcgtat tgtttttgac cgctaaaagc gtctggcggt aatagcgatc gaaatcttca
    16141 ttcgcgaaca ggtgattcag tacttcttct tcggtctgct tacgcagcca ttccaccgat
    16201 tgttgttgcc gcacttgttg taaacgacca ctggcagtta gcgcggtttt gaagtcgatg
    16261 atggcgtgcc agatctcatc gattccacgt ttttccagtg cgctacaagt cagaccccgt
    16321 ggctgccatt cgtcgtattt acgtcgcaga atatgcaggg cactctcgta catatgccgg
    16381 gcaatggcga cattggtatg gttatcgcca tcgtctttgt tgataacgag cagatcagcc
    16441 acttccatca gccctttttt aatgccctgc agatcatcgc cgccaccggc aatttgcaac
    16501 gagataaaac agtccaccat gcgggcgact tccgtttccg actgcgcgat gccaaccgtt
    16561 tcgacaatca ctacgtcata acccgccgct tcgcataaca gcattaattc ccgtgctcgc
    16621 tgactggcac cgcccagatg accggaggag ggtaccgggc gaataaacgc cgcctcggca
    16681 cgcgccaggt cattcatgcg ggttttatcc ccgagaatgc taccgccagt gaccgggctg
    16741 ctgggatcga ccgcaataac agcaaccttt aatccctcac gaatcaacaa catgccaaag
    16801 gcttcaagaa aggtactttt ccccgcgccg ggtgtgccgg taacgcccag tcgcagggcg
    16861 ttaccacaga acggcataat agcgtcaagc agctgtgtac ttagcgcctg atgacgtggg
    16921 tgacggcttt ccaccagcgt catggcttgg gcgagcgtgg cacgctcacc ctgacgtaag
    16981 cggcgaatac tttctgccag cgtggcttca ttaatcatga tgctggctta tcagattcag
    17041 tacgtcgcgc acactgtcga gcataggtgt acctggacca taaatcgccg ccacgccgcg
    17101 ctcttgcagg aaggcgtaat cctgcggcgg aatgacgcca cccgcgacca cgcagatatc
    17161 ttcgcgtccc cattttttca gcgcttcgac cagttccggg atcagcgttt tatgaccggc
    17221 agccagtgag gatgcgccca ctacgtgaac gtcgttttct acggctaggc gggcgatctc
    17281 ttcaggtgta gagaacatcg ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct
    17341 ggcgatcact ttcgcgccgc gatcgtgtcc atcctggccc atcttagcga tcagaatgcg
    17401 cgggcgacga ccattgtcgg caaggaactg ctccgtttgc gcaacaatgg catcgaactc
    17461 ggaggccgat ttctcagact gatgatagct ttgcgcaatc acgccggtaa cacactggct
    17521 tggcaccaga taacggtcga aagcgacttc cagcgcatcg gaaatttcac ccagggtggc
    17581 gcgaacgcga gcggcattaa cagcggcagc cagcaggttt tcgttatgct gtgcggcgtg
    17641 agtcagggcg ttcaacgcgg cggttacggc ggcatcatca cgggtggcgc gaatgcgttc
    17701 cagcgaagca atttgctcgt tacgcaccat cacgttgtcg atctcaagta catcggtttc
    17761 gtcttcgtga tccagtttgt acttgttgac accaacgatg acacgcttgc cctggtcgat
    17821 cagcgactgt tcgcgcgctg aggcctcttc gatcattcgt tttggcagac ctgcttcgat
    17881 cgctttcgcc atgccaccgg cttcgtcgat ctgttggata atagctctgg cttgtttgac
    17941 gatttgatcg gtcagcgact caatgtaata ggatccggcc agtggatcga cggtgtggca
    18001 gagttctgat tcttcctgga tgatgatctg ggtgttgcgg gcaatgcgtg ctgagaaatc
    18061 ggtaggcaaa ccaagcgctt cgtcaaaggc gttggtatgc agtgactgag taccgcccag
    18121 cgtcgcagcc agtgcttcaa tggtggtgcg gataacgttg ttatacggat cctgttcagt
    18181 caggctccag cctgaggtct ggcagtgggt acgcagcgcc agtgatttcg gatcctgtgc
    18241 gccaaatcca ctgaccgctt cgctccataa ataacgtgcc gcacgcaaca tggcgacgtt
    18301 cataaacaga tccatgccga taccgaagaa taacgacagg cgaggagcga agtcatcaat
    18361 tttcagtccg gcagagattg ctgctttgat gtactcaatc ccatcggcga gcgtaaatgc
    18421 tacctgctgc acgcagttgg cacccgcttc ccccatgtgg taaccgctga tactgatggt
    18481 attaaatcgc ggcatgttgc cggaacacca ggcgatgatg tcggcgataa tgcgcattga
    18541 cggttttggt gggtaaatat aggtgttgcg gcagaggtac tctttgagaa tatcgttttg
    18601 aatggtgccg gtcagtttat caggtgtaac accttgctct tctgcggcga cgatataaaa
    18661 cgccagtact ggtagcactg cgccattcat ggtcatcgaa accgacattt tatccagcgg
    18721 gatctggtcg aacaggactt tcatatcttc cacggtgtcg atagcgacgc ccgctttgcc
    18781 gacgtcaccc gccacgcgtg ggttatcgga gtcgtagcca cggtgggtgg caaggtcaaa
    18841 cgcaacggaa agaccttttt gcccggcggc caggttacgg cgataaaaag cgttggactc
    18901 ttttgctgtt gaaaaaccag catactgacg gatggtccac ggttgggcgg tatacatagt
    18961 ggcacgcggg ccacgaacgt agggcggcaa accaggaagg gtacctgtca cctccagatt
    19021 atcgagatcg gcttcggtat acagcggctt gatggcgatc ccttccgcgg tttgctgaac
    19081 cagcgagtcg acagttttct cccgacggct caattccttg ttggcaagct gttgccactc
    19141 ctgctcgtta gacattattt gctccgtaaa acgcaatcaa aatgagggta tttgcgctta
    19201 cggtgagctt tttacgtggg tttgtcgcca gcaaaaaaaa tgcattcaga cgtcgttttt
    19261 tgccgcctga gatttgtcta ttttgtgatg tgattcaagg aatacaaaaa gaagtgagga
    19321 caacgcctga tatgtgctat caggcattat ttgatggatt aatcctgacg aaggactttg
    19381 tgaccataat cgagtaacgc atcagtgact ttacgcatca tgcggctttc aggagcaaag
    19441 cggtgccagt agagcatccg tcgttgaaat agcccaggcg ttaagtcaat cagttcaccg
    19501 ctggccagct ctttctcgat ttgcaggtgc gggatcatac agcaggtggt gccctggcga
    19561 gcaagttgta cgaacgcttc tgaagaatta acgatatggc agggtacgct gccaggagga
    19621 agatcgaagt tttgctgcaa aaaggcctgg tgcatatcgt caagatggtc aaacgcgacc
    19681 actggcgctt tcaataatgc cgaacgcgtt acgccgttag gaaaatattt ttcggcaaac
    19741 ggttttgagc tgacgaacag atagtcgagc gcaccgagtt tatcgacaag acaactcggc
    19801 agcgcctgat gttgaatact caccgcgccg accacttcgc cgcggcgcag acgttcctga
    19861 gtacgggttt catcttctac ctgcaagttg aggcggatag gcgaatcagc caacacagga
    19921 gccagtgccg gaagcaacca cgtcgccaga ctgtcggcgt tgaccgccag tgaaagcagc
    19981 agcggagtcg aaccggtttg ttcatcgccc agccactctt cttccagcaa ctccacctgg
    20041 cgcagcagtg ccagcagttt ttgcccttgt tccgtcgggc gcggcggtac ggtacgcacc
    20101 aacagcggct gcccgaacat attttccagt tgcttaatgc gctgtgagac ggctgattgt
    20161 gtaatgcaca gcttttgtgc cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt
    20221 gcctgtaatg ttctgtagtc cgggcgtttc attgctgttg tctgctcctg gtaaatgctt
    20281 tttcctgcac tatgacacaa ttttgtgtca ggttgcatat accgaagccg tcggcgggta
    20341 acatttgtat caatggttaa acgcgcagct taaaagatca gggatttgcg ctattgcatt
    20401 tcgcttagcg acttcgcgcc ctggttatgt tgaatggcgt ggcgttattg cctctgttcg
    20461 caccgtggag cggcataccc agaccgttgt atgctttatt tgcggggcga aaggggatgc
    20521 ccgccattgc gcgatatttt atggatgagt taaccacccg gcttgccaac ggggtctgaa
    20581 tcgctttttt tgtatataat gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg
    20641 caggatgaat tgaaaaaagc agtaggatgg gcggcacttc agtatgttca gcccggtacc
    20701 attgttggtg taggtacagg ttccaccgcc gcacacttta ttgacgcgct cggcacgatg
    20761 aaaggccaga ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa actgaaaagc
    20821 ctcggcattc acgtttttga tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc
    20881 gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt
    20941 gaaaaaatca ttgcttcggt tgcggaaaaa tttatctgta ttgccgacgc ctccaagcag
    21001 gttgatattc tgggtaaatt cccgctgcca gtagaagtta tcccgatggc acgtagtgca
    21061 gtggcgcgcc agctggtgaa actgggcggt cgtccggaat accgtcaggg cgtggtgacc
    21121 gataatggca acgtgatcct cgacgtccac ggcatggaaa tccttgaccc gatagcgatg
    21181 gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg gcttgtttgc taaccgtggc
    21241 gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac
    21301 gggggaacct cccccgttaa aaaaattctc ttcattaaat ttggtgacat gtgtcacgct
    21361 tttaccaggc aattgtcgat tgctctaaat aaatcctcta aaccagcata ttcatccaag
    21421 aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat tgccgcaata
    21481 ttattttttg atatgttgaa aggcggatgc aaatccgcac acaacatttc aaaagacagg
    21541 attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa
    21601 ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt
    21661 cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc
    21721 ggcctgcgat cccgtaccca tctgactgaa gacgtgatca acgccgcaga aaaactggtc
    21781 gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc
    21841 gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga gctggtgatt
    21901 ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcaccgtggc
    21961 gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc
    22021 ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt
    22081 tacttttatg atattgaaaa caaactgccg ctgggcaacg ccactcaggt acagcatctt
    22141 tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtccacc
    22201 aaaaatatga tgggcgcgaa agagatttcg ctaatgaagc ccggctcgct gctgattaat
    22261 gcctcgcgcg gtactgtggt ggatattccg gcgctgtgtg atgcgctggc gagcaaacat
    22321 ctggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag cgatccattt
    22381 acctctccac tgtgtgaatt cgacaacgtc cttctgacgc cacacattgg cggttcgact
    22441 caggaagcgc aggagaatat cggcttggaa gttgcgggta aattgatcaa gtattctgac
    22501 aatggctcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcatggtggt
    22561 cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc taactgcgct gaacaaaatc
    22621 ttcgccgagc agggcgtcaa catcgccgcg caatatctac aaacttccgc ccagatgggt
    22681 tatgtagtta ttgatattga agctgacgaa gacgttgccg aaaaagcgct gcaggcaatg
    22741 aaagctattc cgggtaccat tcgcgcccgt ctgctgtact aattcccctt ctctgaaaat
    22801 taacgggcag gtcactgact tgcccgtttt tttatccctt ctccacaccg ttgccgcgtt
    22861 gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc aatgcgttgt
    22921 gtttatttaa tggggattta cgatggcggg acagcatggg gctgtaacgg taaagccctc
    22981 accgaagcga gggcttaaag gagaagggtt atgatgcgac ttgtcatcat actgattgta
    23041 ctgttactga taagtttcgg agcttattaa cagtcagtct caagggagga gtcatcctcc
    23101 cttaccctta ctcactaaat taggtcaaag aatcaacgat gtcaatcagg gcgatgcggg
    23161 gtgcatcgcc ctgaaccgaa ctttttgata gccacttcat cgcccacgtt tactgccgta
    23221 atagatcctt tttcccaaaa attatctctg ttttgcatgg ggtttttcga gcacgctcga
    23281 agaaaagatt taaaggtaga tcaatgcgtt gtggttattt aaagagagct tacgatagct
    23341 gggcagcatg gggctgtaac ggtaaagccc tcaccgaagc gagggcttga aggagaaggg
    23401 ttatgatgcg acttgccatc atactgattg tactgttact cataagtttc ccagcttatt
    23461 aacagtcagt cacaggggag gagcaatcct cccttaccct tactcactaa attaggttaa
    23521 agaatcaacg atgtcaatca gggcgatgca ggtgtatcgc ccttaccact cccagacttt
    23581 cgacggtgta accaccgcag gaagagggat atcccactct tcaacgggga gtttttccac
    23641 caactgacaa tcatgcgcat aacccaccgg ttgcgtttta tagtgctgcc agttttgtaa
    23701 ggtccgatca taaaaaccac cgcccattcc cagacgctga ccgtactcat caaaggcgac
    23761 cagcggtgtg atcagcacgt ctaatcggga aaggggtagc acgtcacgca catccagttt
    23821 tggctcatgg atcttcaacc tgttcatcac cagttcgctt tgcggatggt aattcaggaa
    23881 cagcaaatta ccggcactaa agggatgcaa aactggaaga tatacgcgct taccggcgcg
    23941 ccagagttgt tctattagcg gctgggtgtc gagttcacca tcaaaagaga ggaatacagc
    24001 gaccgtatgc gccatcacca ccgggggata agtcatcatc cgggtagcgg cttgttgacc
    24061 catttccagc tgttgttccg gcgttaacgc acgacgacgt tgccgaatca ttttgcggat
    24121 ttcttgtcgg gataatgtca gtgggagctc tggtagttgc gtcatggctg gtgccagata
    24181 agaagggaat ctccgagatg ccgccgcagg ctgtaaccct tgaacccttg gttcaaggtg
    24241 aatgtgtcgt cgcagtttta aggcttctcg gacgaaccga gcatgctcac caaccgcgga
    24301 gcgccacatt cttgtggtat gaaatatcgg ctcaggggac tggcccgctt gcgaacatct
    24361 cagagaaatt ttgtcttcac ggttactcta ccacagtaaa ccgaaaagtg tcattcaaag
    24421 ttttggttag ttttttcggt gatgcgacct tgttcaagta acgcttgttc tatggtctgc
    24481 tgcagcatcc gaatacgctg ttccatactt gccgcgtagt cacgagtctt tgctttttct
    24541 tgcgctaact catagctgat attcaatgcg gcaatgaaga ccaactgttc agtatttgtg
    24601 actctagtgc gttctttcag atcttgcaac cgttggttca gatcgtccgc tgcctgattc
    24661 aacgcatccc tttggtcagg cgggcagttc acacgcagtg aacggccaaa aatttggata
    24721 tcgacgggtt gtgcagacat gccaccttcc tgctgattga ctgcgctgcc ttcgtcttta
    24781 gacccatggt ctgcgaaggg gcgacactat agctaccctg atgagaagag acaagccctt
    24841 ttctggtcca ccagggccca aagtggtagc atatcatgaa tattcctccc tttgacgacg
    24901 aatgcttatg tctatacaga acgaaatgcc tggttacaac gaaatgaacc agtatctgaa
    24961 ccaacaaggg acgggtctga cccctgccga gatgcatggt ttaatcagtg ggatgatatg
    25021 tggcggtaac gatgacagct catggctgcc gctacttcac gacctgacga acgaaggcat
    25081 ggctttcggt catgagctgg cacaggcact gcgtaaaatg cactctgcca ccagcgatgc
    25141 cctgcaggat gacggcttcc tttttcagct ttatctgcct gatggcgatg atgtcagcgt
    25201 tttcgatcgg gctgatgcac tggcaggttg ggtcaatcac ttcctgcttg gtcttggcgt
    25261 tacgcaaccg aagctggaca aagtgaccgg cgaaaccggt gaagccatcg acgatctgcg
    25321 taacatcgcg cagctgggct acgacgaaga cgaagatcag gaagagcttg aaatgtcgct
    25381 tgaagagatc atcgaatacg ttcgtgttgc cgcgctgtta tgccacgaca cctttactca
    25441 tccgcaaccg accgcgccag aagtacaaaa accgactcta cactaaaaac aaaaaacgta
    25501 aggagagtgt tatgagtgag atatcccggc aagagtttca gcgtcgccgt caggccctgg
    25561 tggagcaaat gcaacccggc agcgccgcgc tgatttttgc tgcaccagaa gtaacacgta
    25621 gcgccgacag cgaatacccc tatcgtcaga acagtgactt ctggtacttc accggcttta
    25681 acgaaccgga agcggtgctg gtgctgatta aaagcgatga cactcataac cacagcgttc
    25741 tgtttaaccg cgttcgcgac ctgacggcgg agatctggtt tggccgtcgc ttaggccagg
    25801 atgccgcgcc agagaaactg ggcgttgacc gcgcactggc attcagcgaa atcaatcagc
    25861 aactttatca actacttaac ggcctggatg tggtttacca tgcccagggc gaatatgcat
    25921 atgctgatga aatcgtgaac agtgcgctgg aaaaactgcg taaaggttcg cggcaaaatc
    25981 tcaccgcacc ggcaacgatg atcgactggc gtcctgttgt tcatgaaatg cgcctgttca
    26041 aatcgccaga agagattgcc gtactccgcc gcgcgggaga aatcaccgcc atggcacata
    26101 cacgggcgat ggaaaaatgc cgtccgggaa tgttcgagta ccatctggaa ggcgaaattc
    26161 accacgaatt taaccgccac ggtgcgcgct atccttccta taacaccatt gtaggcagcg
    26221 gtgaaaacgg ctgcattctg cactacaccg aaaacgagag tgaactgcgc gacggtgacc
    26281 tggtgttgat tgacgcgggt tgtgaataca aaggttacgc gggcgatatt acccgcacct
    26341 tcccggtcaa cggcaaattc acccaggccc agcgtgaaat ctacgacatt gtgctggagt
    26401 ctctcgaaac cagcctgcgc ctgtatcgtc cgggaacttc cattctggaa gtcactggtg
    26461 aagtggtgcg catcatggtt agcggcctgg taaaactcgg catcctgaaa ggtaatgttg
    26521 atgaactgat cgctcagaac gcccatcgtc ctttctttat gcatggcctt agccactggt
    26581 taggactgga tgtccatgac gttggcgttt atggtcagga tcgctcgcgc attctggaac
    26641 cggggatggt actgaccgta gagcctggtc tgtatatcgc gccggatgcg gatgtgccag
    26701 aacaatatcg cggtatcggc attcgtattg aagacgacat tgtgattacc gaaaccggca
    26761 acgaaaacct caccgccagc gtggtgaaaa agccggaaga aatcgaagcg ttgatggctg
    26821 ctgcgagaaa gcaatgagcg taatcatcgt cggtggcggc atggcgggcg cgacgctggc
    26881 gctggctatt tcccggttaa gtcacggggc gctgccggta catttgattg aagcgactgc
    26941 gccagagtca catgctcatc cgggctttga tggacgagcg atagcgctgg cggcgggtac
    27001 ctgtcagcaa ctggcgcgca tcggcgtctg gcaatctctg gctgattgcg caaccgccat
    27061 cactaccgtg catgtcagcg atcgtggtca tgctggattc gtcaccctcg ccgcagaaga
    27121 ttaccaactg gcggcgctgg ggcaggttgt cgaattgcat aatgtcgggc aacggctgtt
    27181 tgcattgctg cgaaaagcac ctggcgtaat gctgcattgc cccgatcgcg tagctaacgt
    27241 tgcccgtact cagagtcatg ttgaagtgac gctggagagc ggcgagatgc tgacgggccg
    27301 cgtgctgata gccgctgatg gcacccattc agcgttagcc accgcgtgcg gcgttgattg
    27361 gcagcaggag ccttacgaac aactggcagt gattgccaac gttgctactt ctgttgcgca
    27421 cgaaggccgc gcttttgaac gcttcacgca acatggcccg ctggcgatgt tgccgatgtc
    27481 tgacggacgc tgttcgctgg tctggtgtca tccactggaa cggcgcgaag aggtgctgtc
    27541 gtggagcgac gagaagtttt gccgtgaact ccagtctgcc tttggctggc gactggggca
    27601 aattacccac gccggtaaac gcagtgctta tccgctggca ttaacccgcg ccgccagacc
    27661 gattacccat cgcactgtgc tggtgggcaa tgcggcgcaa actctgcacc cgattgccgg
    27721 gcaagggttt aacctcggta tgcgagatgt catgagcctt gcggaaaccc tgactcaggc
    27781 gcatgagcgc ggagaagaca tcggtgatta cggcatattg tgccgctatc agcagcgtcg
    27841 acagagcgat cgcgaagcaa ccatcggcgt cacggacagc ctggtacatc tttttgccaa
    27901 ccgttgggca ccgctggttg tcgggcgcaa catcggactg atgacgatgg aattattcac
    27961 cccggcacgc gatgtgctgg cgcagcgcac cctcggttgg gtggcgcgtt gaaggcggtt
    28021 ttaaggaata aacaatgcaa agtgttgatg tagccattgt tggcggcggc atggtggggc
    28081 tggcggttgc ctgcggctta caggggagcg gcttacgcgt tgccgtactg gagcagcgcg
    28141 taccggaacc tctggcggta gatgcactac cacaactgcg cgtttcggct atcaatgccg
    28201 ccagcgaaaa attactcacc cgtcttggcg tctggcagga cattctcgct cgtagggcca
    28261 gttgttatca cggcatggaa gtgtgggaca aagacagttt tggtcacatc tcgtttgacg
    28321 atcaaagcat gggctatagc catcttgggc acatcgttga aaactcagtg attcactacg
    28381 cgctgtggaa caaagcgcag cagtcgtcag atattactct gttggccccc acagaattac
    28441 agcaggtcgc ctggggagaa aatgaaacct tcctgacgct gaaagatggc agcatgttaa
    28501 cggcgcgtct ggtgattggc gcggacggtg ctaattcctg gttgcgcaac aaagcagata
    28561 ttccgctgac tttctgggat tatcagcatc acgcgctggt agcgaccatt cgcacggaag
    28621 aaccgcatga tgcggtggcg cggcaggttt tccatggcga aggcattctg gcctttttac
    28681 cgcttagcga tccgcatctt tgctcgattg tctggtcact gtcgccagag gaagcgcagc
    28741 ggatgcagca ggcaagtgaa gacgaattta atcgcgcgtt aaatatcgct tttgataatc
    28801 gccttggctt atgcaaggtt gagagcgcgc gtcaggtgtt cccgctgacg gggcgttatg
    28861 cgcgccagtt tgccgcacac cgtctggcgc tggtgggcga cgccgcgcat accattcacc
    28921 cactggcggg gcagggcgtt aatctcggat ttatggacgc cgcagagctg attgctgaac
    28981 tgaaacggtt gcatcgtcag ggtaaagaca tcgggcagta catttacctg cgccgctatg
    29041 aacgtagccg caagcacagc gcggcgctga tgctggctgg tatgcagggt tttcgcgatc
    29101 tcttttccgg cgctaatccg gcgaaaaaac tgctgcgtga tattggtttg aaactggccg
    29161 acacgcttcc tggcgttaag ccgcaactta tccgccaggc aatgggatta aacgatttgc
    29221 ctgaatggct gcgttaaaaa tttctcctct gttgtttatt tgatacccat cacactttca
    29281 tctcccggtt ttttcgccgg gagattttcc tcatttgaaa taaactaatt tcacctccgt
    29341 tttcgcatta tattttctaa taccatgatt ttttgattta gtgttttttg acattttttt
    29401 agctcttaat attgtcttat tcaaattgac tttctcatca catcatcttt gtatagaaat
    29461 tggtgtattt tttggttttt tattctgtcg cgatttttgc attttttaac cataagctaa
    29521 tgtgatgatc aattttacct tatggttaac agtctgtttc ggtggtaagt tcaggcaaaa
    29581 gagaacgatt gcgttgggga ccgggagtgg ctccgatgct gggttttgtg gtgataattt
    29641 caccatgaaa aagttgtcag ccccgcttat tcaatgagga caagatggca caacagactc
    29701 ctttgtacga acaacacacg ctttgcggcg ctcgcatggt ggatttccac ggctggatga
    29761 tgccgctgca ttacggttcg caaatcgacg aacatcatgc ggtacgtacc gatgccggaa
    29821 tgtttgatgt gtcgcatatg accatcgtcg atctccgcgg cagccgcacc cgggagtttc
    29881 tgcgttatct gctggcgaac gatgtggcga agctcaccaa aagcggcaaa gccctttact
    29941 cggggatgtt gaatgcctct ggcggtgtga tagatgatct catcgtctac tactttactg
    30001 aagatttctt ccgcctcgtt gttaactccg ccacccgcga aaaagacctc tcctggatta
    30061 cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg tgatgacctt tccatgattg
    30121 ccgtgcaagg gccgaatgct caggcaaaag ctgccacact gtttaatgat gctcagcgtc
    30181 aggcggtgga agggatgaaa ccgttctttg gcgtgcaggt gggcgatctg tttattgcca
    30241 ccactggtta taccggtgaa gcgggctatg aaattgcgct gcccaatgaa aaagcggccg
    30301 atttctggcg tgcgctggtg gaagcgggcg ttaagccctg cggtctgggg gcgcgtgaca
    30361 cgctgcgtct ggaagcgggc atgaatcttt atggtcagga gatggacgaa actatttctc
    30421 ctttagccgc caacatgggc tggactatcg cctgggaacc gacagatcgt gactttatcg
    30481 gtcgtgaagc cctggaagtg cagcgtgagc atggtacaga aaaactggtt ggtctggtga
    30541 tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg ctttaccgat gcgcagggca
    30601 accagcatga aggcattatc accagcggta ctttctcccc gacgctgggt tacagcattg
    30661 cgctggcgcg cgtgccggaa ggtattggcg aaacggcgat tgtgcaaatt cgcaaccgtg
    30721 aaatgccggt taaagtgaca aaacctgttt ttgtgcgtaa cggcaaagcc gtcgcgtgat
    30781 ttactttttt ggagattgat tgatgagcaa cgtaccagca gaactgaaat acagcaaaga
    30841 acacgaatgg ctgcgtaaag aagccgacgg cacttacacc gttggtatta ccgaacatgc
    30901 tcaggagctg ttaggcgata tggtgtttgt tgacctgccg gaagtgggcg caacggttag
    30961 cgcgggcgat gactgcgcgg ttgccgaatc ggtaaaagcg gcgtcagaca tttatgcgcc
    31021 agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc gattccccgg aactggtgaa
    31081 cagcgaaccg tatgcaggcg gctggatctt taaaatcaaa gccagcgatg aaagcgaact
    31141 ggaatcactg ctggatgcga ccgcatacga agcattgtta gaagatgagt aacggcttta
    31201 ttcctcttct gcgggagagg atcagggtga ggaaaattta tgcctcaccc tcactctctt
    31261 cgtaaggaga gaggttcaca attcactgca cgtttcagga accatcgctc atgacacaga
    31321 cgttaagcca gcttgaaaac agcggcgctt ttattgaacg ccatatcgga ccggacgccg
    31381 cgcaacagca agaaatgctg aatgccgttg gtgcacaatc gttaaacgcg ctgaccggcc
    31441 agattgtgcc gaaagatatt cagcttgcga caccaccgca ggttggcgca ccggcgaccg
    31501 aatacgccgc actggcagaa ctcaaggcta ttgccagtcg caataaacgc ttcacgtctt
    31561 acatcggcat gggttacacc gccgtgcagc taccgccggt tatcctgcgt aacatgctgg
    31621 aaaatccggg ctggtatacc gcgtacactc cgtatcagcc tgaagtctcc cagggccgcc
    31681 ttgaagcact gctcaacttc cagcaggtaa cgctggattt gactggactg gatatggcct
    31741 ctgcttctct tctggacgag gccaccgctg ccgccgaagc aatggcgatg gcgaaacgcg
    31801 tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc caatgtgcat ccgcaaacgc
    31861 tggatgtggt ccgtactcgt gctgaaacct ttggttttga agtgattgtc gatgacgcgc
    31921 aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt acagcaggta ggcactaccg
    31981 gtgaaattca cgactacact gcgcttatta gcgaactgaa atcacgcaaa attgtggtca
    32041 gcgttgccgc cgatattatg gcgctggtgc tgttaactgc gccgggtaaa cagggcgcgg
    32101 atattgtttt tggttcggcg caacgcttcg gcgtgccgat gggctacggt ggcccacacg
    32161 cggcattctt tgcggcgaaa gatgaataca aacgctcaat gccgggccgt attatcggtg
    32221 tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc gatgcagact cgcgagcaac
    32281 atattcgtcg tgagaaagcg aactccaaca tttgtacttc ccaggtactg ctggcaaaca
    32341 tcgccagcct gtatgccgtt tatcacggcc cggttggcct gaaacgtatc gctaaccgca
    32401 ttcaccgtct gaccgatatt ctggcggcgg gcctgcaaca aaaaggtctg aaactgcgcc
    32461 atgcgcacta tttcgacacc ttgtgtgtgg aagtggccga caaagcgggc gtactgacgc
    32521 gtgccgaagc ggctgaaatc aacctgcgta gcgatatcct gaacgccgtg gggatcaccc
    32581 ttgatgaaac caccacgcgt gaaaacgtaa tgcagctttt cagtgtgctg ctgggcgata
    32641 accacgggct ggagatcgac acgctggaca aagacgtggc tcacgacagc cgctctatcc
    32701 agcctgcgat gctgcgcgac gacgaaatcc tcacccatcc ggtgtttaat cgctatcaca
    32761 gcgaaaccga aatgatgcgc tatatgcact cgctggagcg taaagatctg gcgctgaatc
    32821 aggcgatgat cccgctgggt tcctgcacca tgaaactgaa cgccgccgcc gagatgatcc
    32881 caatcacttg gacggaattt gccgaactgc acccgttttg cccgccggaa caggctgaag
    32941 gttatcagca gatgattgcg cagctggctg actggctggt gaaactgacc ggttacgacg
    33001 ccgtttgtat gcagccgaac tccggcgcac agggcgaata cgcgggcctg ctggcgattc
    33061 gtcattatca tgaaagccgc aacgaagggc atcgcgatat ctgcctgatc ccggcttctg
    33121 cgcacggaac taaccccgct tctgcacata tggcaggaat gcaggtggtg gttgtggcgt
    33181 gtgataaaaa cggcaacatc gatctgactg atctgcgcgc gaaagcggaa caggcgggcg
    33241 ataacctctc ctgtatcatg gtgacttatc cttctaccca cggcgtgtat gaagaaacga
    33301 tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca ggtttacctt gatggcgcga
    33361 acatgaacgc ccaggttggc atcacctcgc cgggctttat tggcgcggac gtttcgcacc
    33421 tcaacctgca taaaactttc tgcattccgc acggcggtgg tggtccgggt atgggaccga
    33481 tcggtgtgaa agcgcatctg gcaccgtttg taccgggtca tagcgtggtg caaatcgaag
    33541 gcatgttaac ccgtcagggc gcagtttctg cggcaccgtt cggtagcgcc tctatcctgc
    33601 caatcagctg gatgtacatc cgcatgatgg gcgcagaagg gctgaaaaaa gcaagccagg
    33661 tggcaatcct caacgccaac tatattgcca gccgcctgca ggatgccttc ccggtgctgt
    33721 ataccggtcg cgacggtcgc gtggcgcacg aatgtattct cgatattcgc ccgctgaaag
    33781 aagaaaccgg catcagcgag ctggacattg ccaagcgcct gatcgactac ggtttccacg
    33841 cgccgacgat gtcgttcccg gtggcgggta cgctgatggt tgaaccgact gaatctgaaa
    33901 gcaaagtgga actggatcgc tttatcgacg cgatgctggc tatccgcgca gaaattgacc
    33961 aggtgaaagc cggtgtctgg ccgctggaag ataacccgct ggtgaacgcg ccgcacattc
    34021 agagcgaact ggtcgccgag tgggcgcatc cgtacagccg tgaagttgcg gtattcccgg
    34081 caggcgtggc agacaaatac tggccgacgg tgaaacgtct ggatgatgtt tacggcgacc
    34141 gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca gtaattcact gattcgagta
    34201 ttttctaaag gcgcttcggc gcctttttag tcagatgaca aagtacaaaa gtgctcagac
    34261 agtcccctcg cccctttggg gagagggtta gggtgagggg aacaggccgg cactggcgcg
    34321 aatatttacc ctcaccccga ccctctccct gaaagggcga gggggaaaag cgtgccaaca
    34381 ttgaagattg agccagtttg ttagcaatct caaagatacg tcaacgaatt aatttttctc
    34441 ggaaaaacaa atggctatag cacttgtgac cggtggcagt cgcggcatcg ggcgggcaac
    34501 tgcattactg ttggcgcaag aagggtatac ggtggcggtt aattatcagc aaaacctcca
    34561 cgcggcgcag gaagtgatga acttaataat gcaagccggt ggcaaagcat tcgtgctcca
    34621 ggcggatatc agcgacgaaa accaggtcgt tgcgatgttt acagcaatcg atcagcacga
    34681 tgaaccgcta gcagcgctgg tcaataacgc cgggatcttg tttacccagt gcaccgttga
    34741 aaaccttacc gcagagcgaa tcaaccgagt actttccacc aacgtgacgg gatattttct
    34801 ctgctgccgc gaggcggtaa aacgcatggc gcttaaaaat ggtggcagtg gcggcgctat
    34861 cgtcaatgtc tcttcggtgg cctcacggtt gggttcgcca ggggaatatg ttgattacgc
    34921 ggcatcgaaa ggggcgattg attcgttaac caccggacta tcgctggaag tcgccgcgca
    34981 ggggatccgc gttaactgcg tgcggccagg gtttatttat accgaaatgc acgccagcgg
    35041 cggcgagcct ggacgcgtcg atcgcgttaa gtcgaacatc cccatgcagc gtggtggaca
    35101 ggcagaagag gtcgcgcagg ccattgtctg gctactaagt gataaagcct cttacgtcac
    35161 gggaagtttt atcgatttgg cgggcgggaa ataaaacagg gaagttgtct gaccggatgc
    35221 aacaagtatt gcatccggta cttcatcgac ttaaagcttc tcgccgttgc tggcaatcac
    35281 ctctttgtac cagttaaagc tcttcttacg tgaacgcgac atatcgccag taccgtcgtc
    35341 atgtttattc acatagataa agccgtagcg tttgctgtac tgcccggtag tgaatgacac
    35401 gcagtcgatg cagccccacg gtgtgtagcc catcagatcc acaccatcgt aagtcactgc
    35461 ttttttcatc tcttcaatat gggcgcgcag gtagtcaatg cggtagtcgt cgttgatgct
    35521 gccatcttct tccactttgt cgtaagcgcc aaaaccgttt tcgacaataa acagcggcct
    35581 ctgataacgc tcatacagtt cgcaaagggc atagcgcaga cctactggat caatctgcca
    35641 gccccagtca gacgctttaa catacgggtt cggtacgctg ccttcaaaac cagagatcgc
    35701 atcgccggtg ccgccttcgg ccttcactgc gttggtcatg taatagctga aaccaagata
    35761 atcgcaggtg ccttcgcgca gtacatcgag atcgccgtct tccattttga tattaaatcc
    35821 gcgacgttcc cactcgttca atacatagga cgggtaatag ccgcatagct gcacatcggt
    35881 aaagacgtag cgttcgcgca tcgactcctg agcgaacatc acatcgtccg ggttacatga
    35941 gtagggatag agcggcacca tcgccagcat acagccgact ttcatctccg ggttaatgcg
    36001 acgcgcggct ttcactgcca gggcgctggc gacaaactgg tgatgcagca cctggtacat
    36061 cgtctcttcc gggttgtcat gctcggtata caccacgccg gagcagcagt aaccaaacag
    36121 cggcgcacgc cagttacgct ggttgtttat ttcgttgaag gtcatccagt atttgacttt
    36181 atgcttgtag cgttcgaata ccacttccgc gaaacggaca aagaaatcaa cgaatttacg
    36241 gttggtccag ctaccgtatt gctgcaccag atgcagcggc atttcaaagt gggagagggt
    36301 tatcaccggt tcgatgttgt atttcagcag ttcatcgaac agagagtcgt aaaacttcag
    36361 cccttcttcg tttggctgag cttcatcacc ttttggaaaa atacgcgtcc aggcgatgga
    36421 agtccgaaaa catttgaagc ccatttcggc aaatagcttg atgtcttcct tgtagtgacc
    36481 ataaaaatca acggcttcat ggtttggata gtattttcct ggcaagactt ctttggtgat
    36541 ttcgcgcggc acgccgtgtg cgccacaggt cagaacgtca caaatgctcg gtcctttgcc
    36601 gcctttgttc cagccgcctt cgacctgatg agcggcaacg gcgccacccc ataagaaatc
    36661 tttcggtaag gtgagttttt tcactatcat aggctccaga tcagtcaatt cttacaggaa
    36721 atagtaccat gcagccaaac gacatcactt ttttcaacgt ttccaggatg acattctggc
    36781 tgggcgtaaa accatcacca tccgcgacga gtctgaatcg cacttcaaaa cgggtgatgt
    36841 gcttcgtgtc ggacgttttg aagatgacgg ttatttttgc acgattgaag tcaccgcaac
    36901 ctcaaccgta acgctggata cactgacgga aaaacatgca gagcaggaaa atatgaccct
    36961 gcctgaactg aaaaaggtca ttgctgacat ctatcccggt cagacacaat tttatatgat
    37021 tgaatttaaa tgtctttaaa ttgaactcac gcctgttagt tgaaatttcg catttaattt
    37081 catgattttt aggagattag tctcaaatca gaatttattt tagctaacag gtgttcactg
    37141 gaactattct cagttacgct ggagaggtat acatgtgcgt gtacgtttct ctggagtaag
    37201 ttatggttca gaagcccctc attaagcagg gatattcgct ggcagaggaa attgccaaca
    37261 gcgtcagtca cggcattggg ctggtgtttg gtatcgttgg gctggtgttg ctactggttc
    37321 aggcggtgga tctcaatgcc agcgccacag cgataaccag ttacagcctc tatggcggca
    37381 gtatgatcct gctgttcctc gcttcgacgc tctatcacgc cattcctcat caacgggcaa
    37441 aaatgtggct gaagaaattt gaccattgcg ctatttacct gttgattgcc ggaacctaca
    37501 cgccgttttt gctggtgggg ctggattctc cgttagcgcg cgggttgatg attgttatct
    37561 ggagcctagc attgctgggg attctgttca aactgaccat cgcgcaccga ttcaaaattt
    37621 tatctctggt gacttatctg gcgatgggct ggctgtcgct ggtggtaatt tatgaaatgg
    37681 cagttaagct cgcggcgggc agcgttacct tactggcggt aggcggcgtg gtttattcgc
    37741 tcggggtgat tttctacgtc tgcaaacgca ttccatacaa ccatgccatc tggcacggct
    37801 tcgtgctcgg cggtagtgtg tgccactttc tggcgatcta tttgtatatt gggcaggcgt
    37861 aatgggtatg cctttgtagg cctgataaga cgcgtcagcg tcgcatcagg catcggagcg
    37921 caaccgccgg atgcggcgtg aacgccttat ccgatctacg ggcctcgtcc ctgttgtgat
    37981 aaaggcatca aatcccagac atgataagac gcgtcagcgt cgcatcaggc gataaatgcc
    38041 tgatgcgata cagagattta ctcttcgagc gaatacggca gcggctcgat atgcagtgta
    38101 ttcgcatcgt cgcgtacgcg gaagatgctg tccggttcca tatcgttatt catgacgacc
    38161 tgtaccacga cctgaccatc ttccagtttt accgcagcca gcaccgtacc ggtacgacgc
    38221 cagttctcgc ccattttcag ctctaagtct tcaccagctt ccggcagtcg gctggcacta
    38281 cctgccagca accagagcgc gcgtttgttg gcaccacgga attttgctcg cgccaccatc
    38341 tcttgtccgg tataacagcc tttcttaaag ctgataccgc ccagcgcctg gagattggtc
    38401 gcctgtggga taaactgccc gctgttggcg gcatcaatca ccgggaaacc agcttcaatg
    38461 tttaatgcca gccactgttg gctattgttc agttccgctt caccgcgcag tttatcggtc
    38521 agcatattag cagtagcttc atcggttacg atcaggaaac gttctgccgg gtgttcaaac
    38581 catagcaaag tggtcgcgcc ttctttgact acctgttttt ctttcgaagg cagttcgcta
    38641 aagagatttg ccagcgcggc gcgcgcctga aaaccggcaa caccaagcag cacacgctcg
    38701 tcgtctggcg cgatggtcac tttagagaat accgcatatt ttttcagttc agtcagctgc
    38761 ggttcacgca cgctgcgccg ttcaatccat gcaaagccat cgccgtcgcg gaacagacgt
    38821 aaattgctcc acattttacc tttggcgtcg caatgggcgg cgagcaggtg ctgatcttct
    38881 gccatctggc tgacatctgc tgtcacctga ccctgcatat atttttcgct gtccgcgcca
    38941 gtaatggtgg caagcgccca gtcatcaagc gtcatcagcg tcagcggtaa acgggcagaa
    39001 gccgtaggct gacgtggagg aaaaggtgta aaagccataa tagagtcctg agttgcttaa
    39061 cggcgtatta atgactaatg gtaaaagagc tatgaggcaa tgcaagtgct ttcactacgc
    39121 gatctgtgcc ttctgcgagg cgactgccaa aaaaatgaag cccttgaatt gttgtgaatt
    39181 aatttcgcga tcgcgatggt gtttactgat attcccgtaa aagaagtcga aaaacacgtt
    39241 acaatagccg ggtactggat tttcgtgaga caggaagaag atggacatta acaacaaagc
    39301 ccgaattcat tgggcatgcc gccgtggtat gcgcgaactc gatatttcaa tcatgccgtt
    39361 tttcgaacat gagtacgaca gcttaagcga tgacgaaaaa cgcatcttta ttcgtctgct
    39421 ggaatgtgac gatccggacc tgtttaactg gctgatgaat cacggtaaac cggccgatgc
    39481 agaactggaa ataatggtcc gactcatcca gacacggaac cgggaacgtg gtcctgtggc
    39541 aatctgattt gcgcgtctcc tggcgcgcac agtggctttc cttgctgatt catgggctgg
    39601 ttgccgctgt tattttactc atgccctggc cgctcagtta caccccgtta tggatggtgt
    39661 tactttcgct ggtggtattt gattgcgttc gcagccagcg gtgtattaat gctcgccagg
    39721 gggaaattcg cttgttgatg gacgggcgtt tgcgttggca agggcaggag tggagcatcg
    39781 tcaaagcacc gtggatgatt aagagcggca tgatgctgcg tttacgttct gatggcggta
    39841 aacggcaaca tttatggctg gcagccgaca gcatggacga agccgaatgg cgggatttac
    39901 ggcggatttt gttgcaacaa gagacgcaaa gataaagatg acggcaaagc atatgcgcta
    39961 agccgtcagt gagatcaggc gtaatgctct gccatttcgt tgaggatttg ctcgcaccag
    40021 ctctgaatac gctcgtcgct aaggtcatac tggttagttt catccagcgc cagacccacg
    40081 aacagttgcc cgtcagcaat caccggtttc gggctggtaa attcatatcc ttccgttggc
    40141 cagtagccga cgaacttcac gcctttggtc gagagtttgt catgcagcat accgagcgca
    40201 tcgaggaacc actcgccgta tcccagttga tcgccaagcc catacagcgc aataatttta
    40261 ccttcaaggt tcaggtcgtc gagctgatcc cagacggctt cccagtcttc ctggatttca
    40321 ccaaaatccc aggtcgggat acccagaatg agcacatcgt actgctccat taatttcggg
    40381 gagtcgtcct tgaggttatg taaggtcacc agttctgggc cgataatatc gcggattttt
    40441 tctgccgcca tttcggtgta acaggtgctg gaaccgtaaa aaagacccat attcataacg
    40501 taagcttctc gattctgctg tggcttttgc agctagtgta tcagaatcgc tttagtcacg
    40561 gcacaataag gcataatgga tcaaatgagt gaccataagg ggcgcaagtg aaacaggagc
    40621 tggcacgcat cgagcagttt cttgatgctc tgtggctgga aaaaaatctg gctgaaaata
    40681 cgttgaacgc ttaccgtcgc gatctgtcaa tgatggtgga gtggttgcat caccgcgggt
    40741 tgacgctggc gacggcgcaa agtgacgatt tgcaggcatt actggcagaa cggctggagg
    40801 gcgggtataa agccaccagc tcagcgcgtt tgctgagtgc agtgcgccga ttgttccagt
    40861 atctttatcg cgaaaagttt cgtgaagacg atcccagtgc gcatctcgct tcaccgaaat
    40921 tgccccagcg tttgccaaaa gatttaagcg aagcgcaggt cgaacgttta ttacaggcgc
    40981 cattaattga tcagccactg gagctacgcg ataaagccat gcttgaagtg ttgtatgcta
    41041 ccgggctgcg tgtctctgaa ctggtcgggc tgacaatgag tgatatcagc ctgcgtcagg
    41101 gcgtagtgcg ggtcattggt aaaggcaaca aagagcgcct ggtgccgtta ggggaagagg
    41161 cggtttactg gctggaaacc tatctggaac atgggcgtcc gtggctgctg aatggtgtgt
    41221 caattgacgt gttgtttccc agccagcgtg cgcagcagat gacgcgacag accttctggc
    41281 accgcattaa acattatgcc gtgctggcgg gtatcgacag cgaaaagctg tcaccgcatg
    41341 tgttgcgtca cgcttttgcc actcatttat taaatcatgg tgcggattta cgcgtggtgc
    41401 agatgctact gggccacagc gatctttcca ccacgcaaat ttatacgcat gtcgctaccg
    41461 agcgtctgcg gcaacttcat caacagcatc acccgcgggc gtgatgtctg aaaagaacgg
    41521 gaagatttat gaagaaaggt tttatgttgt ttactttgtt agcggcgttt tcaggctttg
    41581 ctcaggctga tgacgcggca attcaacaaa cgttagccaa aatgggcatc aaaagcagcg
    41641 atattcagcc cgcgcccgta gctggcatga agacagttct gactaacagc ggtgtgttgt
    41701 acatcaccga tgatggtaaa catatcattc aggggccaat gtatgacgtt agtggcacgg
    41761 ctccggtcaa tgtcaccaat aagatgctgt taaagcagtt gaatgcgctt gaaaaagaga
    41821 tgatcgttta taaagcgccg caggaaaaac acgtcatcac cgtgtttact gatattacct
    41881 gtggttactg ccacaaactg catgagcaaa tggcagacta taacgcgctg gggatcaccg
    41941 tgcgttatct tgctttcccg cgccaggggc tggacagcga tgcagagaaa gaaatgaaag
    42001 ctatctggtg tgcgaaagat aaaaacaaag cgtttgatga tgtgatggca ggtaaaagcg
    42061 tcgcaccagc cagctgcgac gtggatattg ccgaccatta cgcacttggc gtccagcttg
    42121 gcgttagcgg tactccggca gttgtgctga gcaatggcac acttgttccg ggttaccagc
    42181 cgccgaaaga catgaaagaa ttcctcgacg aacaccaaaa aatgaccagc ggtaaataat
    42241 tcgcgtgaaa caacagatac aacttcgtcg ccgtgaagtc gatgaaacgg cagacttgcc
    42301 cgctgaattg cctcccttgc tgcgccgttt atacgccagc cggggagtac gcagtgcgca
    42361 agaactggaa cgcagtgtta aaggtatgtt gccctggcag caactaagcg gtgtcgaaaa
    42421 ggccgttgag atcctttaca acgcttttcg cgaaggaacg cggattattg tggtcggtga
    42481 tttcgacgcc gacggcgcga ccagcacggc tctaagcgtg ctggcgatgc gctcgcttgg
    42541 ttgcagcaat atcgactacc tggtaccaaa ccgtttcgaa gacggttacg gcttaagccc
    42601 ggaagtggtc gatcaggccc atgcccgtgg cgcgcagtta attgtcacgg tggataacgg
    42661 tatttcctcc catgcgggag ttgagcacgc tcgctcgttg ggcatcccgg ttattgttac
    42721 cgatcaccat ttgccgggcg acacattacc cgcagcggaa gcgatcatta accctaactt
    42781 gcgcgactgt aatttcccgt cgaaatcact ggcaggcgtg ggtgtggcgt tttatctgat
    42841 gctggcgctg cgcacctttt tgcgcgatca gggctggttt gatgagcgcg gcatcgcaat
    42901 tcctaacctg gcagaactgc tggatctggt agcactgggg acagtagcgg acgtcgtgcc
    42961 gctggacgct aataatcgca ttctgacctg gcaggggatg agtcgcatcc gtgccggaaa
    43021 gtgccgtcca gggattaaag cgctgctgga agtggcaaat cgcgatgcac aaaaactagc
    43081 cgccagcgat ttaggttttg cgctgggacc gcgtctcaat gctgccgggc gactggatga
    43141 tatgtccgtt ggtgtggcgc tgttgctgtg cgacaacatc ggcgaagcgc gcgtgctggc
    43201 aaatgaactc gatgcgctaa accagacgcg aaaagagatc gaacaaggaa tgcaggttga
    43261 agccctgacc ctgtgcgaga aactggagcg cagccgtgac acgctacccg gcgggctggc
    43321 aatgtatcac cccgaatggc atcagggcgt tgtcggtatt ctggcttcgc gtatcaaaga
    43381 gcgttttcac cgtccggtta tcgcctttgc gccagcaggt gacggtacgc tgaaaggttc
    43441 cggtcgctcc attcaggggc tgcatatgcg tgatgcgctg gagcgattag acacactcta
    43501 ccccggcatg atgctcaagt ttggcggtca tgcgatggcg gcgggtttgt cgctggaaga
    43561 ggataaattc gaactctttc aacaacggtt tggcgaactg gttactgagt ggctggcccc
    43621 ttcgctattg caaggcgaag tggtgtcaga cggtccgtta agcccggccg aaatgaccat
    43681 ggaagtggcg cagctgctgc gcgatgctgg cccgtggggg cagatgttcc cggagccgct
    43741 gtttgacggt catttccgtc tgctgcaaca gcggctggtg ggcgaacgtc atttgaaggt
    43801 gatggtcgaa ccggtcggcg gcggtccact gctggatggt attgctttta atgtcgatac
    43861 cgctctctgg ccggataacg gcgtgcgcga agtgcaactg gcttataagc tcgatatcaa
    43921 cgagtttcgc ggcaaccgca gcctgcaaat tatcatcgac aatatctggc caatttagcg
    43981 tcatcttctc tataaaaaag agcgtggatt gggtacaatc ccgctcttat caccgcattt
    44041 tgactagctc aataaaagaa atcagaccat gtttgaaatt aatccggtaa ataatcgcat
    44101 tcaggacctc acggaacgct ccgacgttct tagggggtat ctttgactac gacgccaaga
    44161 aagagcgtct ggaagaagta aacgccgagc tggaacagcc ggatgtctgg aacgaacccg
    44221 aacgcgcaca ggcgctgggt aaagagcgtt cctccctcga agccgttgtc gacaccctcg
    44281 accaaatgaa acaggggctg gaagatgttt ctggtctgct ggaactggct gtagaagctg
    44341 acgacgaaga aacctttaac gaagccgttg ctgaactcga cgccctggaa gaaaaactgg
    44401 cgcagcttga gttccgccgt atgttctctg gcgaatatga cagcgccgac tgttacctcg
    44461 atattcaggc ggggtctggc ggtacggaag cacaggactg ggcgagcatg cttgagcgta
    44521 tgtatctgcg ctgggcagaa tcgcgtggtt tcaaaactga aatcatcgaa gagtcggaag
    44581 gtgaagtggc gggtattaaa tccgtgacga tcaaaatctc cggcgattac gcttacggct
    44641 ggctgcgtac agaaactggc gttcaccgcc tggtgcgtaa aagcccgttt gactccggcg
    44701 gtcgtcgcca cacgtcgttc agctctgcgt ttgtttatcc ggaagttgat gatgatattg
    44761 atatcgaaat caacccggcg gatctgcgca ttgacgttta tcgcgcgtcc ggcgcgggcg
    44821 gtcagcacgt taaccgtacc gaatctgcgg tgcgtattac ccacatcccg accgggatcg
    44881 tgacccagtg ccagaacgac cgttcccagc acaagaacaa agatcaggcc atgaagcaga
    44941 tgaaagcgaa gctttatgaa ctggagatgc agaagaaaaa tgccgagaaa caggcgatgg
    45001 aagataacaa atctgacatc ggctggggca gccagattcg ttcttatgtc cttgatgact
    45061 cccgcattaa agatctgcgc accggggtag aaacccgcaa cacgcaggcc gtgctggacg
    45121 gcagcctgga tcaatttatc gaagcaagtt tgaaagcagg gttatgagga accaacatgt
    45181 ctgaacaaca cgcacagggc gctgacgcgg tagtcgatct taacaatgaa ctgaaaacgc
    45241 gtcgtgagaa gctggcgaac ctgcgtgagc agggaattgc cttcccgaac gatttccgtc
    45301 gcgatcatac ctctgaccaa ttgcacgcag aattcgacgg taaagagaac gaagaactgg
    45361 aagcgctgaa catcgaagtc gccgttgctg gccgcatgat gacccgccgt attatgggta
    45421 aagcgtcttt cgttaccctg caggatgtgg gtggccgcat tcagctgtac gttgcccgtg
    45481 acgatctgcc ggaaggcgtt tacaacgagc agttcaaaaa atgggacctc ggcgacatcc
    45541 tcggcgcgaa aggtaaactg ttcaaaacca aaaccggcga actgtctatc cactgtaccg
    45601 agctgcgtct gctgaccaaa gcactgcgtc cgctgccgga taaattccac ggcttgcagg
    45661 atcaggaagc gcgctatcgt cagcgttatc tggatctcat ctctaacgat gaatcccgta
    45721 acacctttaa agtacgctcg cagatcctct ctggtattcg ccagttcatg gtgaaccgcg
    45781 gctttatgga agttgaaacg ccgatgatgc aggtgatccc aggcggtgcc gctgcgcgtc
    45841 cgtttatcac ccaccataac gcgctggatc tcgacatgta cctgcgtatc gcgccggaac
    45901 tgtacctcaa gcgtctggtg gtcggtggct tcgagcgtgt attcgaaatc aaccgtaact
    45961 tccgtaacga aggtatttcc gtacgtcata acccagagtt caccatgatg gaactctaca
    46021 tggcttacgc agattacaaa gatctgatcg agctgaccga atcgctgttc cgtactctgg
    46081 cacaggatat tctcggtaag acggaagtga cctacggcga cgtgacgctg gacttcggta
    46141 aaccgttcga aaaactgacc atgcgtgaag cgatcaagaa atatcgcccg gaaaccgaca
    46201 tggcggatct ggataacttc gactctgcga aagcgattgc tgaatctatc ggtatccacg
    46261 ttgagaagag ctggggtctg ggccgtatcg ttaccgagat cttcgaagaa gtggcagaag
    46321 cacatctgat tcagccgacc ttcattactg aatatccggc agaagtttct ccgctggcgc
    46381 gtcgtaacga cattaacccg gaaatcacag accgctttga gttcttcatc ggtggtcgtg
    46441 aaatcggtaa cggctttagc gagctgaatg acgcggaaga tcaggcgcaa cgcttcctgg
    46501 atcaggttgc cgcgaaagac gcaggtgacg acgaagcgat gttctacgat gaagattacg
    46561 ttaccgcact ggaacatggc ttaccgccga cagcaggtct ggggattggc atcgaccgta
    46621 tggtaatgct gttcaccaac agccatacca tccgcgacgt tattctgttc ccggcgatgc
    46681 gtccggtgaa ataagcatta cgttatgctc acaaccccgg caaatgtcgg ggttttttat
    46741 ttaagctggg taaatgcaga taatcgtatt ctggcttcgc gatttgtcgc ctgcatcacc
    46801 atccacggac tgaacgccca cggcgtggca tcaataccgc gtaatacatc agctaaatca
    46861 caccattgat aatccatcac ttcatcatcg ttgatctgta acgcactggt ggtgcgtgcg
    46921 gcaaataccg gacacacttc attttccaca atgcctctcg gatcggtggc gcggtagcga
    46981 aagtcaggat agatagattc aggaggcgta atttccacgc caagctcata acggcaacgg
    47041 cggatcaccg cgtcttcgct gctttctccc agttgtgggt gcccacaaac cgagttagtc
    47101 cacacgccag gccacgcttt tttgctcagt gcgcggcggg taactaataa ttgccccttg
    47161 gcattaaaca gccaactgga gaacgcgaga tgtaagaggg tgtctgccgt gtgtgcggca
    47221 tacttttcca gcgtacccgt gggaactccc tgtgcattca ataaaatgac gtgttccgtt
    47281 tgcataattt ctcacatgta attctgatca ctcatcatta tagataattg aagagtgaaa
    47341 tgattaacca agagagcgtt tatcgacaac cgcccgtttt gagcggatgc cgacaggtaa
    47401 aaaccattta aaaaagtagc atcatatatt tgaagcgact ttcaatggcc attgcaacac
    47461 agtcagaaat gacaggaata gagagagtaa aaagcaacaa cagactgaat atagcgcgca
    47521 ttaaaaaatt catattattc tccatgctca tttttcttca gcttgcgcat cagaagccac
    47581 atacgtactg tcctgacttc taaatcttta tcagagacgg attccttcac caggtcagca
    47641 tgatgctgat agccattaaa gaagacgttc agcacaacgg cgctaagggt tgcgagcata
    47701 atgccgctat gtagcagcgg ttgcagtacg gccggtaact tagaaaagaa atcgtgagag
    47761 agcgtcggag tcatgccaac gccgagactg atcgccacaa tatagaggtt gtaacggttg
    47821 gtggtgtagt tacagcgcga cagaattcga atccctgtcg ccagtaccat gccgaacatc
    47881 actagaccag cgccgcccag cacaaattgc ggaatggagg ctaccagcac cgccattttt
    47941 ggcaccatgc caaacaggat taatataatt cccgaagaaa tacacaccca gcggctatga
    48001 acgcgcatca cgctaaccag gccaacgttt tgagaaaatg acgtgtgggg gaagctgtta
    48061 aacgtgccgc ctatcattgt ccctacgcca tcgacacgca gcccgcaaat aatatcgtgt
    48121 gaagagagtt tacgaccgac tatttcaccc agtgccagga acatccccat cgactcgata
    48181 aacacgatga ttaacacggc agtcatggtc agaatggaaa cgggatcgaa aatcggcata
    48241 ccaaacgaca tcggggtgac aatcgcaaac cacgaggcat catgtagccc ggataaattg
    48301 acttcattca tcatccacga aagtaaaaag ccaaatacaa tccccagtaa tacggcgacg
    48361 ttggacataa atcctttcgc atagcgagta atgagcaaga taaaaattaa gacggcaaag
    48421 gagataccta aataaacggg attaccatat tgcggattcc ctttacctcc agcggcccag
    48481 tcaataccca cctgaatgat gctaagccca atagaagtaa taaccacacc ggtaaccagt
    48541 ggcgggaata aaggcatcaa gcgaccaata agtggcgcta ataatgtggt gataaaaccc
    48601 gcggcgatag tggcaccaaa tatccccagc aggccgatat ccgggttcat cccaatggct
    48661 atcattggtg ttacagcagc aaaggtcacc gacataatca ccggcaggcg gatccccata
    48721 aagcggccga taccgataca ttgcaataat gtgacgatcc cgcagcaaaa gagatccgag
    48781 ctaatgagca tcgcaatagc atcttttgag aggcccagtc ggtcaccaat cataagagga
    48841 acagcgactg cacctgcgta catgacaagg acgtgttgca gaccgagtat gatcagcttt
    48901 cctggtgata atatgcggtc aacctcatca gttgggcggt cttgcccaga agatgaggga
    48961 agttgggaat ctatggcgct catcagtgtt tctcctttcg cccgccagaa taaaaagcaa
    49021 ccgtttacgt taatgaaact gtctaaacac atgctcgtgg agtgccagtt tttgcgagga
    49081 aggggagtta gagacaggaa ttgcggaggc aatcacataa tcgtgcttaa accacgaaaa
    49141 tgacaagaga aactgacaat ggaattatct gtaattttct catattgata tttcattaat
    49201 cattctggca tgtcgcactc tcgcatttaa tcgtttttat ct
//
LOCUS       DAJTPG010000017        49048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTPG010000017 DAJTPG010000000
VERSION     DAJTPG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1154)
                     /gene="setB"
                     /locus_tag="OO230_001062"
     CDS             complement(<1..1154)
                     /gene="setB"
                     /locus_tag="OO230_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5690909.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1522..2652
                     /gene="fruB"
                     /locus_tag="OO230_001063"
     CDS             1522..2652
                     /gene="fruB"
                     /locus_tag="OO230_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5690910.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2652..3590
                     /gene="fruK"
                     /locus_tag="OO230_001064"
     CDS             2652..3590
                     /gene="fruK"
                     /locus_tag="OO230_001064"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5690911.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3607..5298
                     /gene="fruA"
                     /locus_tag="OO230_001065"
     CDS             3607..5298
                     /gene="fruA"
                     /locus_tag="OO230_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5690912.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5722..6663
                     /locus_tag="OO230_001066"
     CDS             5722..6663
                     /locus_tag="OO230_001066"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5690913.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6651..7589
                     /gene="psuG"
                     /locus_tag="OO230_001067"
     CDS             6651..7589
                     /gene="psuG"
                     /locus_tag="OO230_001067"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5690914.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7683..8933
                     /locus_tag="OO230_001068"
     CDS             7683..8933
                     /locus_tag="OO230_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5690915.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9004..9698)
                     /gene="yeiL"
                     /locus_tag="OO230_001069"
                     /pseudo
     CDS             complement(9004..9698)
                     /gene="yeiL"
                     /locus_tag="OO230_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9829..10770
                     /gene="rihB"
                     /locus_tag="OO230_001070"
     CDS             9829..10770
                     /gene="rihB"
                     /locus_tag="OO230_001070"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5690916.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10870..12120
                     /locus_tag="OO230_001071"
     CDS             10870..12120
                     /locus_tag="OO230_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5690917.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12176..13264)
                     /locus_tag="OO230_001072"
     CDS             complement(12176..13264)
                     /locus_tag="OO230_001072"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5690918.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13267..14124)
                     /gene="nfo"
                     /locus_tag="OO230_001073"
     CDS             complement(13267..14124)
                     /gene="nfo"
                     /locus_tag="OO230_001073"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5690919.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14198..15247)
                     /locus_tag="OO230_001074"
     CDS             complement(14198..15247)
                     /locus_tag="OO230_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5690920.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15346..16227
                     /locus_tag="OO230_001075"
     CDS             15346..16227
                     /locus_tag="OO230_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5690921.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16433..17902
                     /gene="lysP"
                     /locus_tag="OO230_001076"
     CDS             16433..17902
                     /gene="lysP"
                     /locus_tag="OO230_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5690922.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18194..20185
                     /gene="cirA"
                     /locus_tag="OO230_001077"
                     /pseudo
     CDS             18194..20185
                     /gene="cirA"
                     /locus_tag="OO230_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20217..21052)
                     /gene="yeiG"
                     /locus_tag="OO230_001078"
                     /pseudo
     CDS             complement(20217..21052)
                     /gene="yeiG"
                     /locus_tag="OO230_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21310..21978
                     /gene="folE"
                     /locus_tag="OO230_001079"
     CDS             21310..21978
                     /gene="folE"
                     /locus_tag="OO230_001079"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5690923.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21995..23071
                     /locus_tag="OO230_001080"
     CDS             21995..23071
                     /locus_tag="OO230_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5690924.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23213..24253
                     /gene="galS"
                     /locus_tag="OO230_001081"
     CDS             23213..24253
                     /gene="galS"
                     /locus_tag="OO230_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5690925.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24533..25531
                     /gene="mglB"
                     /locus_tag="OO230_001082"
     CDS             24533..25531
                     /gene="mglB"
                     /locus_tag="OO230_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5690926.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25592..27112
                     /gene="mglA"
                     /locus_tag="OO230_001083"
     CDS             25592..27112
                     /gene="mglA"
                     /locus_tag="OO230_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5690927.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27128..28138
                     /gene="mglC"
                     /locus_tag="OO230_001084"
     CDS             27128..28138
                     /gene="mglC"
                     /locus_tag="OO230_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5690928.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28174..29387)
                     /gene="preA"
                     /locus_tag="OO230_001085"
                     /pseudo
     CDS             complement(28174..29387)
                     /gene="preA"
                     /locus_tag="OO230_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29381..30619)
                     /gene="preT"
                     /locus_tag="OO230_001086"
     CDS             complement(29381..30619)
                     /gene="preT"
                     /locus_tag="OO230_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5690929.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30813..31052)
                     /locus_tag="OO230_001087"
     CDS             complement(30813..31052)
                     /locus_tag="OO230_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5690930.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31055..31774)
                     /gene="sanA"
                     /locus_tag="OO230_001088"
     CDS             complement(31055..31774)
                     /gene="sanA"
                     /locus_tag="OO230_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5690931.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31924..32808)
                     /gene="cdd"
                     /locus_tag="OO230_001089"
     CDS             complement(31924..32808)
                     /gene="cdd"
                     /locus_tag="OO230_001089"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5690932.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32938..33633)
                     /locus_tag="OO230_001090"
     CDS             complement(32938..33633)
                     /locus_tag="OO230_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5690933.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33630..34028)
                     /locus_tag="OO230_001091"
     CDS             complement(33630..34028)
                     /locus_tag="OO230_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5690934.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34267..35214
                     /gene="dusC"
                     /locus_tag="OO230_001092"
     CDS             34267..35214
                     /gene="dusC"
                     /locus_tag="OO230_001092"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5690935.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35585..35668)
                     /gene="yohP"
                     /locus_tag="OO230_001093"
     CDS             complement(35585..35668)
                     /gene="yohP"
                     /locus_tag="OO230_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5690936.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35892..37328
                     /gene="mdtQ"
                     /locus_tag="OO230_001094"
                     /pseudo
     CDS             35892..37328
                     /gene="mdtQ"
                     /locus_tag="OO230_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37381..38142
                     /locus_tag="OO230_001095"
                     /pseudo
     CDS             37381..38142
                     /locus_tag="OO230_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38259..38837)
                     /locus_tag="OO230_001096"
     CDS             complement(38259..38837)
                     /locus_tag="OO230_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5690937.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39007..39594
                     /locus_tag="OO230_001097"
     CDS             39007..39594
                     /locus_tag="OO230_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5690938.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39768..40700
                     /gene="pbpG"
                     /locus_tag="OO230_001098"
     CDS             39768..40700
                     /gene="pbpG"
                     /locus_tag="OO230_001098"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5690939.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40738..42453)
                     /gene="dld"
                     /locus_tag="OO230_001099"
     CDS             complement(40738..42453)
                     /gene="dld"
                     /locus_tag="OO230_001099"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5690940.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42649..44946
                     /gene="bglX"
                     /locus_tag="OO230_001100"
     CDS             42649..44946
                     /gene="bglX"
                     /locus_tag="OO230_001100"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5690941.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45198..46115
                     /gene="osmF"
                     /locus_tag="OO230_001101"
     CDS             45198..46115
                     /gene="osmF"
                     /locus_tag="OO230_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5690942.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46122..47279
                     /gene="yehY"
                     /locus_tag="OO230_001102"
     CDS             46122..47279
                     /gene="yehY"
                     /locus_tag="OO230_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5690943.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47272..48198
                     /gene="yehX"
                     /locus_tag="OO230_001103"
     CDS             47272..48198
                     /gene="yehX"
                     /locus_tag="OO230_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5690944.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48203..48934
                     /gene="yehW"
                     /locus_tag="OO230_001104"
     CDS             48203..48934
                     /gene="yehW"
                     /locus_tag="OO230_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5690945.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48915..49022)
                     /locus_tag="OO230_001105"
     CDS             complement(48915..49022)
                     /locus_tag="OO230_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5690946.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
BASE COUNT        11847 a        12089 c        13172 g        11940 t
ORIGIN      
        1 aacagagtgg cgataatcat caccatcgca aaccagaaca cagcgtgata attccagatc
       61 tcggcgacga tgcccgccac tgatcctgcg atgatccagc ccacgcgcga cgtgttggta
      121 tagagcgtgg tggctgaacc tgcctgaccg ggcatcaaat cctgaaaata gagcatcccg
      181 atgccgccca gaatgccaat aaaaatagca tttagcagct gcaagcccaa cagaatggca
      241 ggtgaatgcg ccatcagcat tcctgcgtaa aaacagacgc cacccacggc agcaacgcgc
      301 attaagaaac gcttacccag acgtttggcg aaatatccgg caatcaacat cgtcgggatt
      361 tccagcccgg cggcggtccc catcatcaca ccggccagtt tctcgggaag atgcagttcg
      421 ttgataataa atagcggcat gttgatgatg tacaggctgt tcgagcccca catcaatgta
      481 caaatgacaa acagcagtag cgtatcgcga cggttacggc gcggcgcttc aaccgtgccg
      541 gtcgccagcg gaagctcttt ttgcatcgac ggtaaaaaca gccacaccat cacgccgcaa
      601 acaataaacg ctaccgctgc gctcagatac attaccgtaa agctgaaacc catcgctaac
      661 gcataagcca gcggtgggcc aatgacccat gccagtgaaa cctgagcgcg taaaaaagag
      721 ctgaacatca ccgcctcacg tccggttttg tcggcatgtt cacgggcaag ggcaaacatt
      781 tgcgggttag cggtcgagcc aaagctgcta agaaagacgc caacgaatag caaaacaaag
      841 tagttgcgat tccaagcaaa aagggtgcag gccagcacgc ctaacaggca gcaaaagaca
      901 atcagcgatt tgcgatcgcc gcgcttatca gagcgcccag cgagaaactg acttaccaga
      961 atcccaatga cagcgctgcc ggtaaagaag aatcccacca tcgccggacg ggcatgtact
     1021 tcatcggtaa ggaaaatact gagtgtcggg gtttgcagag cacccgcaat accggtgaga
     1081 aaggcaacga ttaaaaacgc cgtcgaggtc aggtcaaacg atttcgcgct ggagactgcg
     1141 ggggagttat gcatgctggt cgatcactgg aagaggaaga cgcggagttt acgctgcttg
     1201 tcagaaaata aacagacagc gaaatcaaaa gcgccacgaa taatcaaaat gccatttcac
     1261 tgttgctgat tggctgctga cgctgctgaa atgaaattgc tgataacgga ttttcccatc
     1321 agcaattagg aaaaatgaca aaaaattgtg cagcacatca aacttttgct cataacttta
     1381 cggctttcct tgcgtgctga aacagaagta ttatgctttc ttgaaacgtt tcagcgcgat
     1441 cttgtcttta accctaagct aggttggcgc tttttttctc atagaggctg aatcgtttca
     1501 attcagcaag agaggagaac tatgttccag ttatccgtac aggacatcca tccgggcgaa
     1561 aaggccggag acaaagaaga ggcgattcgc caggtcgctg cggcgctggt gcaggccggt
     1621 aatgtagcag aaggctacgt caatggcatg ctggcgcgcg aacagcaaac ctcaacgttc
     1681 ctcggcaatg gtattgctat tccacacggc actaccgaca cccgcgatca ggtgctgaaa
     1741 accggcgttc aggtatttca gttcccggaa ggcgtcacct ggggtgacgg tcaggtagcg
     1801 tacgtggcaa tcggtattgc tgccagctcg gataaacatc tgggcctgct acgccagctg
     1861 acccacgtac tgagcgatga ttccgttgct gaacaactga agtcagcaac aacagcagaa
     1921 gaacttcgcg cattactgat gggcgaaaag cagagtgagc agctgaagct cgacaacgaa
     1981 atgctgacgc tggatatcgt cgccagcgat ctgctgactc ttcaggcgct gaacgctgcg
     2041 cgtctgaaag aggcgagggc agttgacgcc actttcgtca ccaaagccat caatgaacaa
     2101 ccgctgaatc tcggacaggg tatctggctg agcgatagcg ccgaaggcaa tctgcgtagt
     2161 gcgattgcgg taagccgtgc ggcaaatgct tttgatgtgg acggcgaaac ggcagccatg
     2221 ctggtgagtg tggcgatgaa tgacgatcag cccatcgcgg ttcttaagcg tctcgctgat
     2281 ttgttgctcg acaataaagc tgaccgcttg ctgaaagcgg atgcggcaac gttgctggcg
     2341 ctgctgacca gcgatgatgc gccgaccgac gacgtgttaa gcgcggagtt tgtggtgcgc
     2401 aatgaacacg gcctgcatgc tcgtccaggt accatgctgg tcaataccat taaacaattt
     2461 aacagtgata ttaccgtgac aaaccttgat ggcaccggca aaccggcaaa cggacgtagt
     2521 ctgatgaaag ttgtggcact tggcgttaag aaaggtcatc gcctacgctt taccgcccag
     2581 ggtgcagatg ctgaacaggc gctgaaagcc attggcgacg ctatcgctgc tggtcttggg
     2641 gagggcgcat aatgagcaga cgtgttgcta cgatcaccct taatccggct tatgaccttg
     2701 ttggtttctg cccggaaatt gaacgcggcg aagtgaacct ggtgaaaacc accggtctgc
     2761 atgcggcggg taaaggcatc aacgtggcca aagtattaaa agacctggga attgatgtca
     2821 ccgttggcgg cttcctgggt aaagacaatc aggatggttt tcagcaactg ttcagcgagc
     2881 tgggcattgc caaccgtttc caggttgtac aggggcgcac ccgaattaac gttaagctga
     2941 cggaaaaaga cggcgaagtg accgacttca acttctcggg tttcgaagtc acccccgccg
     3001 actgggaacg ctttgtgact gattctctga gctggctcgg tcagttcgat atggtctgtg
     3061 tcagcggaag cttaccgtca ggcgtcagcc cggaagcgtt caccgactgg atgactcgcc
     3121 tgcgtagtca gtgtccttgc attatctttg atagtagccg tgaagcgtta gtagcaggtt
     3181 tgaaagcggc accgtggctg gtgaaaccta accgccgcga gttggaaatc tgggcaggcc
     3241 gtaaactgcc tgaaatgaaa gatgtgattg aagctgcaca tgcgctacgt gaacaaggta
     3301 ttgcgcatgt tgttatttca ctgggtgccg aaggcgcgct ttgggttaat gcctccggcg
     3361 aatggatcgc taaaccaccg tcagtcgatg tcgtaagcac cgttggcgca ggggattcta
     3421 tggttggtgg cctgatttat ggcttgctga tgcgtgaatc cagtgaacac acactgcgtc
     3481 tggcgacagc tgttgcagcc ctggcggtaa gtcaaagcaa tgtgggtatt accgatcgtc
     3541 cgcagttggc cgcaatgatg gcgcgcgtcg acttacaacc ttttaactga cagcaggaga
     3601 ggcataatga aaacgctgct gattattgac gctaatctcg gtcaggcacg cgcctatatg
     3661 gcgaagaccc tgctgggcgc ggcggcgcga aaagcaaaac tggaaatcat cgacaatccg
     3721 aacgacgcag aaatggcgat tgttctcggt gattccatcc cgaatgacag cgcgctgaac
     3781 ggtaaaaatg tctggctggg tgatatttcc cgggcagttg cgcaccctga gctgttcctg
     3841 agtgaagcca aaggccatgc gaaaccttac actgcgccgg tcactgcgac agcaccggtt
     3901 gccgccagcg gtccgaaacg cgtagttgcg gtgactgctt gcccgactgg cgtagcacac
     3961 acctttatgg cggctgaagc cattgaaacc gaagcgaaaa aacgtggctg gtgggtgaaa
     4021 gttgaaaccc gtggttctgt tggcgcgggt aatgcaatca ctcccgagga agtagccgca
     4081 gcggatctgg tgattgtggc ggcagatatc gaagtggatc tggcgaaatt tgctggtaaa
     4141 ccgatgtatc gtacctctac cggtctggcg ctgaagaaaa cctcgcagga actggataaa
     4201 gcggttgctg aagcaacgcc gtatgaaccg gcgggcaaag ctcaaacggc gaccactgaa
     4261 ggtaagaaag agagtgcagg cgcttatcgt cacttgctaa cgggcgtctc ttacatgctg
     4321 ccgatggtcg ttgctggtgg tctgtgtatc gcgctttctt ttgcttttgg tatcgaagcg
     4381 tttaaagagc cgggtacgtt ggcagcggcg ctgatgcaga ttggtggtgg ttcagccttt
     4441 gcgctgatgg tgccggtact ggcaggttat attgccttct ccattgccga tcgtccgggc
     4501 ctcacgccgg gtctgattgg cggtatgctg gcggtcagca ccggttctgg cttcattggc
     4561 ggtattattg cgggcttcct ggctggttac attgcgaagt taatcagtac gcaactgaaa
     4621 ctgccgcaga gtatggaggc gctgaaaccg atcctgatca ttccgctaat ttccagtctg
     4681 gtggtcggtc tggcgatgat ctacctgatc ggtaaaccag ttgctggcat tctcgaaggg
     4741 ctgactcact ggctgcagac catggggact gcgaatgcgg ttctgctggg ggcgatcctc
     4801 ggtggcatga tgtgtactga catgggcggt ccggtaaaca aagcagcgta cgcattcggt
     4861 gtgggtctgc tgagtactca aacctatggc ccgatggcgg cgattatggc ggcaggtatg
     4921 gtgccaccgc tggcaatggg cctggcaaca atggtggcgc gtcgcaaatt cgacaaagcg
     4981 cagcaggaag gtggcaaagc cgctctggta ttgggactgt gcttcatttc ggaaggtgca
     5041 attccgtttg ctgctcgtga tccgatgcgt gtgctgccgt gctgtatcgt gggtggggcg
     5101 ctgactggcg caatctcaat ggcgattggt gcgaaactga tggcaccgca cggtggtctg
     5161 tttgttctgc tgatccctgg cgctattacg ccggtattgg gttacctggt agcaattatt
     5221 gccggtacgc tggtggcggg tttggcctat gccttcctga aacgtccgga agtggacgca
     5281 gtagcgaaag cagcgtaata aaaggtgtgt tacagggcag aaatttcctg ccctgtaaca
     5341 tttgatgagc gcaaagagaa tgaccagctt gtgaatgtaa ttttatgaat ttaaatggtt
     5401 ttatctttca gtagaaactt caaacgttca tcaaacacta aggttatttc ctcaagggca
     5461 gattattttc tgcccttttt tagtgttcct gttctgtgca tcccgtcaca aattcctcgc
     5521 ttttgtaatt caggcattta atcgcatttt gtgagcctgc tcaaatctgc ccattttgcc
     5581 tctctggcgg taatttactt tacgtaaaac atgcatacta tgagcacatg tttaaatatg
     5641 aacacatgtt taaaccgtct ttgcagtatc tatattcttg tggattgaca gggttggtaa
     5701 ttttttacca gaggacgtgc tatgcgcgaa aaggattatg tcgtaattat aggttcggcg
     5761 aatattgatg tcgccggata ttcacatgaa tcattaaatt atgcggattc aaatccaggt
     5821 aaaataaaat ttacgcctgg tggagtaggg cgcaatattg cacaaaacct ggcgttgctg
     5881 ggtaacaaag cctggctact gagcgccgta ggcagtgatt tttatggtca atcgctgcta
     5941 acgcaaacca atcaatctgg tgtttatgtc gataaatgcc tgattgtgcc gggagaaaat
     6001 acgtcgagtt atttatcatt actcgataat accggtgaaa tgctggttgc tataaatgac
     6061 atgaatatta gcaacgctat tacagctgaa tatctcgcac agcaccgtga atttattcag
     6121 agggcaaagg tcattgtcgc ggactgtaat atcagtgaag aggcactggc atggattctg
     6181 gataatgccg ccaacgtacc cgtatttgtc gatccggttt cagcgtggaa atgtgtcaaa
     6241 gtgcgcgacc gtctaaatca gaaccacact ctgaagccaa accgccttga agcggaaacc
     6301 ctgagtggga ttgcgctgtc agggcgtgac gatgtggcaa aagttgctgc ctggttccat
     6361 caacatggcc tgaaccgact ggtattgagc atgggcggcg acggcgttta ttacagcgat
     6421 atcagcggtg aaaatggctg gtctgcgccg atcaaaacca atgttattaa tgttaccgga
     6481 gcgggcgatg ccatgatggc gggacttgct tcgtgttggg tagacggaat gccgtttgcc
     6541 gaatctgttc gtttcgcaca gggatgttcg tcaatggcgc tctcctgtga atacaccaat
     6601 aaccccgatt tatcgattgc caacgttata tcgttagtgg agaacgcaga atgtctgaat
     6661 taaaaatttc ccctgaatta ttacaaattt ctccggaagt gcaggacgct ttaaaaaaca
     6721 aaaaaccggt tgtggcgctg gaatcgacca ttatttctca cgggatgccg ttcccacaaa
     6781 atgcccagac cgcaattgaa gtagaagaaa ctattcgtaa acagggcgca gtacctgcca
     6841 ctatcgccat tattggcggc gtgatgaaag tgggtttaag caaagaaaaa attgaattac
     6901 tgggtcgtga agggcataac gtgaccaaag ttagtcgtcg cgatttacct tttgttgttg
     6961 ccgccggaaa aaatggcgca accactgtgg cttcaacgat gattattgcg gcgcttgccg
     7021 gaattaaagt atttgccacc gggggaattg gtggtgtgca tcgcggggcg gaacatacct
     7081 tcgatatttc tgccgatttg caagaactgg caaatactaa tgtcaccgtt gtttgtgccg
     7141 gggcgaaatc tattctcgat ttaggattaa ccactgagta tttagaaacc ttcggtgtgc
     7201 cgttaattgg ctatcagact aaagcgctgc ctgcgttttt ctgccgtacc agctcgtttg
     7261 acgtcagcat tcgtctcgac agcgccagtg aaattgcccg tgcaatggcg gtgaaatggc
     7321 aaagcgggct gaacggtggc ctcgtggtag cgaacccgat cccggaacag tttgcgatgc
     7381 cggaggaatc tatcaatgca gccatagatc aagccgtcgc cgaagccgaa gagcagggcg
     7441 ttattggtaa agaaagtaca ccgttcctgc tggcgcgcgt tgctgagctg accagcggtg
     7501 acagcctgaa atccaatatc cagctggtgt tcaacaacgc cattctggcg agtgaaattg
     7561 ccaaagaata ccagcgtctc gcgggttaat ttctgacggg cagaaagccg tcgcccgtac
     7621 tcatcaagca taccaaaacc tggcgctgac gccgggttta ctggcatgaa gggaaaatca
     7681 ttatggatat aatgagaagt gttgtgggga tggtggtgtt actggcaata gcatttctgt
     7741 tgtcagtgaa taaaaagagc atcagtttgc gcacggttgg agccgcactg ctgctgcaaa
     7801 tcgctattgg tggcatcatg ctctacttcc caccgggaaa atgggcagta gaacaggcgg
     7861 cattaggcgt tcataaagtg atgtcttaca gtgatgccgg tagcgccttc atttttggtt
     7921 cgctggttgg gccgaaaatg gatgtcctgt ttgacggtgc gggttttatc ttcgcctttc
     7981 gcgtacttcc ggcgattatt ttcgttactg cgctcatcag tctgctgtac tacattggcg
     8041 tgatggggct gctgattcgc atccttggca gcattttcca gaaagccctc aacatcagca
     8101 aaatcgaatc ttttgttgcg gttaccacta ttttcctcgg gcaaaatgag atcccggcga
     8161 tcgttaaacc gtttatcgat cgcgtgaatc gcaacgagtt gtttaccgca atttgtagcg
     8221 ggatggcgtc cattgctggt tcggtgatga ttggttatgc cggaatgggc gtaccaattg
     8281 actacctgtt agcggcatcg ctgatggcga tccccggcgg tattttgttt gcacgtattc
     8341 ttagcccggc aaccgagcct tcgcaggtca catttgaaaa tctgtcgttc agcgaaacgc
     8401 cgccaaaaag ctttatcgaa gcggcggcga gcggtgcgat gaccgggctg aaaatcgccg
     8461 ctggtgtagc gacggtggta atggcgtttg tcgcaattat tgcgctgatc aacggcatta
     8521 tcggcggaat tggcggctgg tttggtttcg ccaatgcctc tctggaaagt atttttggct
     8581 atgtgctggc accgctggcg tggatcatgg gtgtggactg gagtgatgcc aaccttgctg
     8641 gtagcctgat tgggcagaaa ctggcgatta acgaattcgt cgcttacctg agtttctccc
     8701 catacctgca aacgggcggc acgctggaag tgaaaaccgt ggcgattata tcctttgcgc
     8761 tttgtggttt tgctaacttt ggttctatcg gtgttgtcgt tggcgcattt tcggctattt
     8821 cgccaaaacg tgctccagaa atcgcccagc ttggtttacg ggcgctggca gcagcaacgc
     8881 tttccaacct gatgagtgcg actattgccg ggttctttat tggtctggcg taatgctcga
     8941 tttgtagacc ggataaaacg tattaagcgt tgcatccggc aatcgcgcta ttgcaaataa
     9001 tttttactgc atcatcccgg agaatttatt ctccgggtcc atctccagcg ccagtcctga
     9061 caactgcttt ctgtttttaa ttagatatcc tttcttttaa ttaataaacc gtcgtgaatg
     9121 aattgcgcga gaacatataa aagatgtcga taagaaacgc ctaaatactc tgccgcttgc
     9181 gtatgctttt cgtgataaag atcaccttct tgtgagagta atataaatgc tgccaggcga
     9241 ttcactaacg gaaatgattg attctgagtt aaagaaacaa tattacgata atttttatga
     9301 cttaaggtga cgcaaagttt tcgtaaaaat agggtgtcgt ttaataacag cggacggtaa
     9361 tgtttcatag ggagcgcaag gcaccaacac tcttcaatag cctgcaccgc acgcggttca
     9421 tggtctttgt cgattaactc aatctcgcca ataaaacagg gagcggcaaa gaaatcgatc
     9481 agcgacacgc gaccattggc aagcgtggcg taaagcctgg cgcggcctcg cgtcagataa
     9541 aacagccatg acggctgttg cccttcctgc acaatgtagt cacgcgctaa aaaatgaaac
     9601 agccgcgtat cggctgaaac atccgttgaa aagcaatcct taaacgcaga ttcactcatc
     9661 aattgctgct taaggtcatt attgtggatt tctttcacga tgtttatccg ctatgagatt
     9721 tctcatattt agcatacgta cgctgttgtt aagattcaat cattggcccg gtcgaatttt
     9781 ctttgctgta gaaaaaaata acgcaactgg aaacagagga aataaacaat ggaaaagaga
     9841 aaaattattc tggattgtga gccgggtcat gatgatgcta ttgcaatgat gatggcagcg
     9901 aaacatccgg caatagattt attaggcatc actattgtag cgggtaatca gacgcttgat
     9961 aaaacattaa ttaatggcct gaatgtttgc cagaaactgg agattaatgt tccggtttat
    10021 gcggggatgc cgcaacccat tatgcgtaaa caaatcgttg ccgataatat tcacggtgaa
    10081 accggactgg atggcccggt attcgagccg ctgacccgcc aggcagaaag tacccatgcg
    10141 gtgaaatata tcatcgatac cctgatggca agcgatggcg atatcactct ggtgccggtt
    10201 ggtccgcttt caaatatcgc ggtggcaatg cgtatgcaac ccgcaatcct gcccaaaatc
    10261 cgtgaaattg tgctaatggg cggcgcttac ggtacaggca acttcacccc atctgccgag
    10321 ttcaatatct ttgccgaccc ggaagccgcc cgcgtggtgt tcacctccgg cgttccatta
    10381 gtgatgatgg gcctcgatct taccaaccag accgtttgca ccccggatgt aattgctcgg
    10441 atggaaaggg taggcggccc tgccggagag ctgttcagcg acatcatgaa cttcactctc
    10501 aaaacgcagt tcgaatacta cggcctggct ggcggcccgg tgcatgacgc cacctgcatt
    10561 ggttatctga tcaaccctga tggcattaaa acccaggata tgtacgtcga agtggacgtc
    10621 aacagcggcc cttgctacgg gcgtacggtc tgcgacgaac tgggcgttct tggcaagccc
    10681 gctaatacca aagtcggcat cactattgat accgactggt tctggggctt agtcgaagag
    10741 tgcgtgcgcg gctacatcaa aacccattaa gtgttgataa caagccggga cagtatcgca
    10801 agatgcaggc ccggctattt ctgatgggca gaaagccgtc gcccaaaatg acatgaagag
    10861 ataatcacta tggatgtcat gagaagtgtt ctgggaatgg tggtactgct ggcaattgcg
    10921 tttttactgt cagtaaacaa gaagaagatc agcctgcgta ccgttggcgc ggcgttagtg
    10981 ttacaggtcg tgattggcgg gattatgctt tggttaccgc cagggcgttg ggtcgcagaa
    11041 aaagtcgctt ttggcgtcca caaagtgatg gcgtacagcg acgcaggtag cgcatttatc
    11101 ttcggttctc tggtcggacc gaaaatggat accttatttg atggcgcagg atttatcttt
    11161 ggtttcaggg tattaccggc aattatcttc gtcactgcac tggtgagtat tctctactac
    11221 atcggtgtga tggggatctt aattcgcatt ctcggcggta tcttccagaa agctttaaat
    11281 atcagcaaga tcgagtcatt cgtcgcggtc accaccattt tcctcgggca aaacgaaatt
    11341 ccggcgatcg tcaaaccctt tatcgatcgt ctgaatcgca atgaattatt tacagcgatt
    11401 tgtagtggca tggcctcgat tgctggttcg acaatgattg gttacgccgc actgggcgta
    11461 cctgtggaat atttgctggc ggcatcgtta atggcgatcc ctggcgggat cttgtttgcc
    11521 cgcctgttaa gcccggctac ggaatcttcg caggtttctt ttaataacct ctctttcacc
    11581 gaaacaccgc caaaaagcat tattgaagcc gatgcgacag gggcaatgac cgggctgaaa
    11641 atcgccgccg gtgtagcgac agttgttatg gcatttgtcg ccatcattgc gttaattaac
    11701 ggtattatcg gcggcgttgg cggctggttt ggttttgaac atgcctcgct ggagtccatt
    11761 ttaggttacc tgctggcccc acttgcgtgg gtaatgggtg ttgactggag tgatgcaaat
    11821 cttgccggga gtttgattgg acagaaactg gcaataaatg aatttgtcgc ttatctcaat
    11881 ttctcaccct atctgcaaac ggctggcact ctggatgcta aaaccgtggc gattatttcc
    11941 ttcgcgttgt gcggtttcgc taactttggt tctatcgggg tggtggtggg ggcgttttct
    12001 gcggttgcgc cacaccgtgc gccggaaatc gcccagcttg gtttacgcgc gctggcggcg
    12061 gcgacacttt ctaatctgat gagtgccacc attgccgggt tctttattgg tttagcgtag
    12121 tgtgcaattt atgagccgga ttagatgcgg tcaagcgtcg aatccggcaa cattgttaca
    12181 ccattggcac taatgacagc gcgttatcgg cagacagggt agggttgttt aagctgccgc
    12241 tggcgcgtga aattgccgcg caggccatcg caaaacgggc gctgtcacgg aaattgtttc
    12301 cctccagaaa gctatatacc agacccgcca taaaaccatc gtcagcacca aaactgtcta
    12361 ccgtcgtatg cgctggcaca gtcagcaaaa attgttcacc atccttttcg ctgcaataaa
    12421 ctgactcatc gggcaaataa acaaacagtt gctgaacacc ttgctgatgc aatgcattca
    12481 ctgcggcatt acggtcagca tcgctggtga tcgcctgtcc ccataaaatt tccagctccg
    12541 gtaaagtggg tttcagggtg tgaatatgcg ccagccagtg tttgattttg cccgctttga
    12601 attctgaaac ggtatcgaca aacaccggga tctcatcagc gagggtaaag acccattcca
    12661 gcgcctcggc tgtcaggttg caatcagcca gtaccacgcc cgcatgacga agtaaatcgc
    12721 gcgacccgtt cagtagttgc ggtgtcaact gttccagcag atgggtatcg ttaattgcca
    12781 gcacggtttg atcgtctcga ttggcaattg ccagatacgt cgatgtgctt tgaccatgca
    12841 aacgaacgca gccggagaca ttcacgccgg cacggcgcgt ttcttccagg agcatttcgc
    12901 cataaaagtc atcgccaatc actgaaagca aatggacgtc acggcctaac agcgccagat
    12961 tgtgggcgat attgcgtcca acgccgcctg ccgaacaatg aattgtaccg ggatgagaag
    13021 ccgcttgcgg gtaatggata tccgccatcc cgcgaatatc catattgatt gtccccacca
    13081 ctacgcagta ttcctgctcg gtgagaatgt aacctttgtc tttaatccgg cctttgcgca
    13141 ttaaatccat aatatgcgcc gcaacgcgcg aacgactgat ttgcagcatg tccgcaattt
    13201 cgttctgctg aatcagcggg ttacgccgta aaattgcaag gatctccttt tcccggttat
    13261 tcatcttcag gctaccgctt tttcagtttg ttgcgctttc agccaggcga tctcttctgc
    13321 ccagatatcc gggttgatgg tttcaaggat cagcggaatg ccgtcgaaac ggtcgttctg
    13381 cataatccag cggaacgcat catgaccgat attgccttca ccgaggctat gatggcggtc
    13441 aacgcggctg ccaaaggtac ttttcgcatc gttaaggtgc atcccgcgca aatacttaaa
    13501 gccgacaata cgggcaaaat cagcgaacgt tttttcgcat tcggctggag tacgcaaatc
    13561 atacccggca gcgaaagcat ggcaggtatc aatgcagacg ccgacgcggg atttatcttc
    13621 cacgccgtcg ataatcgccg cgagatgttc gaatttaaac cctaagttac tgccctgacc
    13681 ggcggtgttt tcaatcaccg ctgttacacc ttgagtttta tccagcgcaa tgttgatgga
    13741 ttcggcaata cgcgcaaggc aatcctcttc tgaaatctgc atcagatggc tgccagggtg
    13801 gaagttgagc aaagaaagcc ccagttgttc gcaacgctgc atttcatcta taaaggcatc
    13861 gcgcgatttt tccagagctt cagtgaccgg atgtccgagg ttgatcagat aactgtcgtg
    13921 gggaagaatt tgcgccgatg tgtagtgata tttttcacag gcggctttga attcatcgat
    13981 ggtttgcgtc gtgagcggtg cggcacgcca ctgacgttgg tttttggtga acaaggcaaa
    14041 cgcggttgcg tcgatttcgg cggcgcgaat tgcggcattt gccagaccgc cagcagcact
    14101 aacgtgcgct ccaatgtatt tcatgcgagg actcctgtta aacccgctgg aggaaaacgg
    14161 taatgatagc gggttaacag gagcaagaag tagtggttta tgcgatgacg ctttgaatca
    14221 catagttaat cgcaccacca ccaacaatca gccaggcaaa cagtaccagt gccatcagca
    14281 gaggtttcgc cccagctttt ttcagcgcgc tgacgtgagt ggtcagaccc agcgccgcca
    14341 tcgccattgc cagcaggaag gtatccagcg ttaccagcat gttcaccacg ctctgcggta
    14401 acaggtggaa cgagttaaag atggcaacta cgatgaacaa gatggcaaac cacggaatag
    14461 tgattttgct tttctcgccg ctgttcgccc cagacagctg tttaacacgc gccgccagca
    14521 ggatgaggaa cggagccagc atcatcacgc gcagcatttt ggaaataact gctgcgtttt
    14581 ctgcatccgg gctgatggca tgacctgccg ccaccacctg cgccacttcg tgcacagtag
    14641 aaccaatgta gataccgaaa gtttccggac taaaccattg agacatcagc ggatatatcg
    14701 ccgggtagag gaaaatcgcg acggtcccga agataacaac ggttgcaaca gccacggtta
    14761 ctttactggc ttccgctttc actaccggct cagtcgccag taccgcggca gcaccacaga
    14821 tactgctacc ggcaccgatc aaccaactgg tgtgcttatc cagaccaaac actttctgcc
    14881 ccaggaagca agccagcagg aaggtactgg acagcgtcag cacgtcaatg atgatcccac
    14941 tgataccgac atcggcaatt tgcgagaacg tcagacggaa gccataaaga atgataccca
    15001 gacgtaataa atattgcttg gcaaacagca caccaccgtc acagcttttc cagatgtgcg
    15061 gatagatggt gttgcctaaa accatcccca acaagattgc gagggtgagg gcactaaacc
    15121 cggcacccgc aaccgcggga atggaaccac cccacagggc gaccccggtg ataactgcac
    15181 tcagggctaa ccccggaata aaatgccaca gtgtacgatg ttgtttctgt aaggtgatat
    15241 tcgtcataac cctctccttt aaccgggcat aaggttacga ctaactggtt taaaaataaa
    15301 attgattata tatttataat taatctttat aagtggtaag cgactatgca catcaccctc
    15361 cggcagttgg aagtttttgc agaagtattg aaaagtggat caaccaccca ggcgtcggtg
    15421 atgctggcgt tgtcgcaatc agcagtgagc gcagccttga ctgacctgga agggcagctt
    15481 ggcgttcaac tgtttgatcg cgtggggaaa agactggttg ttaatgaaca cgggcggctg
    15541 ctctatccgc gagcgttggc actgcttgaa caggcggttg aaatcgaaca actgtttcgc
    15601 gaagacaacg gcgcgattcg tatctatgcc agtagtacca tcggtaacta cattctgcct
    15661 gcagttatcg cccgttatcg ccatgattat ccgcagttgc cgattgaact tagcgttggg
    15721 aacagccagg acgtgatgca agcggtgctg gatttccgcg ttgatattgg ctttattgaa
    15781 ggaccgtgcc acagcactga aatcatttct gaaccgtggc tggaagacga gctggtggtt
    15841 ttcgccgcgc cgacttcgcc gttggctcgt ggtccggtca ccttagaaca gctggccgct
    15901 gcgccgtgga tcctgcgtga acgcggttcc ggcacgcggg atattgtcga ttatctgttg
    15961 ctgtcacatt taccgaagtt tgagatggcg atggaattag gtaactccga ggcaatcaaa
    16021 catgcggtgc gtcatgggtt gggaattagt tgcctgtcgc gacgtgtgat tgaagatcaa
    16081 ttgcaggcag gcacattaag tgaagttgcg gtccctctgc cgcgcctgat gcgtacgttg
    16141 tggcgtatac atcatcggca aaaacacctt tccaacgcgc tacggcgctt tctggactat
    16201 tgcgatcccg caaatgtgcc gcgttaagtt gctgtacaag aacatgctgg tgctgtgtcg
    16261 atttcgtgac gcagcgcctt cagcatgcat tcgccagaaa agagattggc tgctttactt
    16321 ataatccctg ggcgatcatg aaggtgtctt ataaccgtgt atttctgccg gaaggattgc
    16381 caatcgtctg ctacaatcgc gccatcattt ttaagatgga tagcattttt gtatgggttc
    16441 cgaaactaaa actacagaag cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac
    16501 gatgattgcc attggcggtt ccatcggtac aggtcttttt gttgcctctg gcgcaacgat
    16561 ttctcaggca ggtccgggcg gggcattgct ctcgtatatg ctgattggcc tgatggttta
    16621 cttcctgatg accagtctcg gtgaattggc tgcatatatg ccggtttccg gttcgtttgc
    16681 cacttacggt cagaactatg ttgaagaagg ctttggcttc gcgctgggct ggaactactg
    16741 gtacaactgg gcggtgacta ttgccgttga cctggttgca gcacagctgg tcatgagctg
    16801 gtggttcccg gatacaccgg gctggatctg gagtgcgttg ttcctcggcg ttatcttcct
    16861 gttgaactac atctctgttc gcggctttgg tgaagcggaa tactggttct cactgatcaa
    16921 agtcacgaca gttattgtct ttatcatcgt tggtgtgctg atgattatcg gtatcttcaa
    16981 aggcgcgcag cctgcgggct ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg
    17041 ttttgcggcg atgatcggcg tagctatgat tgtcggcttc tctttccagg gaaccgagct
    17101 gatcggtatt gctgcaggcg agtccgaaga tccggcgaaa aacattccac gcgcggtacg
    17161 tcaggtattc tggcgaatcc tgttgttcta tgtgttcgcg atcctgatta tcagcctgat
    17221 tattccgtat accgatccga gcctgctgcg taacgatgtt aaagacatca gcgtcagtcc
    17281 gttcaccctg gtgttccagc acgcgggtct gctctctgcg gcggcggtga tgaacgcagt
    17341 tattctgacg gcggtgctgt cagcaggtaa ctccggtatg tatgcgtcta ctcgtatgct
    17401 gtacaccctg gcgtgtgacg gtaaagcgcc gcgcattttc gctaaattgt cgcgtggtgg
    17461 cgtgccgcgt aatgcgctgt atgcgacgac ggtgattgcc ggtttgtgct tcctgacctc
    17521 catgtttggc aaccagacgg tatacctgtg gctgctgaac acctccggga tgacgggttt
    17581 tatcgcctgg ctggggattg ccattagcca ctatcgcttc cgtcgcggtt acgtattgca
    17641 gggacacgac attaacgatc tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt
    17701 cgcattcatt ctgtgtctga ttatcacttt gggccagaac tacgaagcgt tcctgaaaga
    17761 taccattgac tggggcggcg tagcggcaac gtatattggt atcccgctgt tcctgattat
    17821 ttggttcggc tacaagctga ttaaaggaac tcacttcgta cgctacagcg aaatgaagtt
    17881 cccgcagaac gataagaaat aagtttcctc ccttccttgc taagccctct caaccgagag
    17941 ggctttttca attccatttc cctgacaaat catgcggata taaaatttaa catttggatt
    18001 gataattgtt atcgtttgca ttatcgttac gacgcaatca aaaaaggctg acaaatcaga
    18061 ggctgttccg gctttctggg atgatcacct gcatacaaat aagtccaccg cgatgctgcc
    18121 gtacgcaagg ggacgtaaag aagatgtgag cgatacccat tttattttcg tagttacctc
    18181 atggagatat ggaatgttta ggttgaaccc tttcgtacgg gtcgggctgt gtttgtccgc
    18241 tatttcttgt gcatggcctg tgttagcggt cgatgatgat ggcgaaacga tggttgtcac
    18301 tgcatcttcc gtggaacaaa atcttaaaga tgcacccgcc agtatcagcg tcattaccca
    18361 ggaagacctg cagcgaaaac cggtacagaa tctgaaggat gtcctcaaag aagtgcctgg
    18421 cgtacaactg acgaacgaag gggataaccg taagggcgtt agtattcgtg gtctggacag
    18481 cagctacacc ctgattcttg tcgacggtaa acgcgttaac tcccgcaatg ccgtcttccg
    18541 ccacaatgat ttcgatctga actggatccc ggtcgattcc atcgaacgta ttgaagtggt
    18601 tcgtggcccg atgtcgtcgc tatacggctc cgatgcgctc ggcggtgtag tgaatatcat
    18661 caccaaaaaa atcggtcaga aatggtcggg taccgttacc gtcgatacca ccattcagga
    18721 acatcgcgat cgcggtgata cctataacgg tcagttcttt accagtggac cattaattga
    18781 tggtgtgctg ggaatgaaag cttacggcag cctggcaaaa cgtgaaaagg atgacccgca
    18841 aaactcaacg accaccgata ccggagaaac gccgcgtatt gaaggattct ccagccgcga
    18901 cggcaatgtc gaatttgcct agacaccgaa tcaaaatcac gattttactg ccggatacgg
    18961 tttcgaccgt caggatcgtg attccgattc gctggacaaa aaccgcctgg aacgccagaa
    19021 ctactgcgtc agccataatg ggcgttggga ttacggcacc agcgaactga aatcctacgg
    19081 tgagaaagtc gagaacaaaa accctggcaa cagcagcccg ataacttccg aaagcaatac
    19141 ggtcgacggc aaatacacgt tgccgctgac ggcgattaat cagtttctca cggttggcgg
    19201 tgaatggcgt cacgacaaac ttagcgatgc ggtgaacctg accgggggaa ccagctccaa
    19261 aacgtctgcc agccagtacg cgctgtttgt ggaagatgaa tggcggatct tcgagccgct
    19321 ggcgctgacg accggcgtgc gtatggacga tcacgaaacc tacggtgaac actggagtcc
    19381 gcgtgcctac atggtttata acgccaccga caccgtaacg gtgaaagggg gctgggcgac
    19441 ggcatttaaa gcaccttctc tgttgcaact tagccctgac tggacgagca attcctgccg
    19501 tggcgcatgt aagattgtgg gtagcccgga tctgaaacca gaaaccagcg aaagttggga
    19561 gctggggctt tactacatgg gtgaagaagg ctggctggaa gggggtgaat ccagcgttat
    19621 cgttttccgt aacgatgtga aagatcgtat cagcatcagc cgtacgtctg acgtcaacgc
    19681 tgcaccgggc taccaaaact ttgttggttt tgagacgggc gctaacggac ggcgcatacc
    19741 ggtatttagc tactacagcg ttaacaaagc tcgtattcag ggcgtggaaa ccgaactgaa
    19801 aattccgttc aacgatgaat ggaaactgtc gatcaactac acctacaacg atggtcgtga
    19861 tgtcagcaac ggcgaaaaca aaccgctatc cgatctgccg ttccatactg ctaacggtac
    19921 gctggactgg aaaccgctgg cgctggaaga ctggtcattc tatgtttctg gtcactatac
    19981 cgggcagaaa cgcgccgaca gcgcgacggc taaaacaccg ggcggttata ccatctggaa
    20041 taccggcgcg gcctggcagg tgactaaaga cgtcaaactg cgcgcaggcg tgctgaacct
    20101 tggcgacaag gatctcagtc gtgacgacta cagctataac gaagacggac gtcgttactt
    20161 tatggcagtg gattatcgct tctgatgaga agatgcccgg cgaaccgggc ggactttcac
    20221 ttcagtaaat actgcgcatg gaagcgcagg tgatcctcta taaaagaggc gatgaagtag
    20281 taactgtgat catatcccgg ctgaatacgc agcgtcatcg gccacgcttt ctggcgcgcg
    20341 gcttctgcca ataccgcagg ttgcaactgg tcggcaagaa actgatcatt atcgccctga
    20401 tcgataagcg tcgggatcgc atcctgcgcg ttactggcat acatcagcgc gcaactatcc
    20461 cattccagca tgcatttttg tcctcaccta aataggtgct aaacgctttg attccccacg
    20521 ggacgctgca cggattcaca atgggcgcaa aggccgaaac gctggtgtat ttacccggat
    20581 ttttcagcgc cataatcagc gcaccgtgac cacccattga gtgaccgcta atggcgcagc
    20641 ggtcgctgac attaaattgc gactgaacca gcgccggtaa ttcatcgcgc agataatcat
    20701 acatccggta atgcgtcgcc cacggcggtt gcgtggcatt aagataaaag cctgcgccct
    20761 ggcctaaatc gtagccatcg tcgttggcaa ccttttcgcc gcgcgggctg gtgtctggca
    20821 tcaccagtac aatccccagt tccgccgcta cccgctgggc acccgccttg gtggtgaagt
    20881 tctcgtcatt gcaggttaat ccggaaagcc agtacagcac tggtggcggg gtgtgatcac
    20941 gagggggggg gagaaagata ctgaacgtca tcgggcagtt taaggtactg gagtcgtgtc
    21001 gccagcgttg ctgccagcct tcaaaacagc ggtgctcttc gagcatttcc atgcgtggct
    21061 ccttgttgtg ttgtttgcaa tttcttcatc ataccgttta ttcaatgtgc tgtgagtaac
    21121 tttcacttcc gtatttgcat aacgatgttt taacatctgc tgatgaaagg cagcggcaat
    21181 tacaataatt atcgctgtga atactggatt atgtgcgccg cctcacgcac aataatcaag
    21241 ctgtaaatca gcttaataac tttgccccca cgcagggtgg aggcgtcaca cctgcaggag
    21301 aaatcataaa tgccatcact cagtaaagaa gcggccctgg ttcatgaagc gttagttgcg
    21361 agaggactgg aaacaccgct gcgcccgccc gtgcatgaaa tggataacga aacgcgcaaa
    21421 agccttattg ctggtcatat gaccgaaatc atgcagctgc tgaatctcga cctggctgat
    21481 gacagtttga tggaaacgcc gcatcgcatc gctaaaatgt atgtcgatga aattttctcc
    21541 ggtctggatt acgccaattt cccgaaaatc accctcattg aaaacaaaat gaaggtcgat
    21601 gaaatggtca ccgtgcgcga tatcactctg accagtacct gtgaacacca ttttgttacc
    21661 atcgatggca aagcgacggt ggcttatatc ccgaaagatt cggtgatcgg tctgtcaaaa
    21721 attaaccgta tcgtgcagtt ctttgcccag cgtccgcagg tgcaggaacg tctgacgcag
    21781 caaattctta ttgcgctaca aacgctgctg ggcaccaata acgtggctgt ctcgatcgac
    21841 gcggtgcatt actgcgtgaa ggcgcgtggc atccgcgatg caaccagtgc cacgacaacg
    21901 acctctcttg gtggattgtt caaatccagt cagaatacgc gccacgagtt tctgcgcgct
    21961 gtgcgccatc acaactgatt aaaaggcagg aaccatggag cgcaacgtca cgctcgattt
    22021 tgttcgcggc gtcgccattc tggggatcct gctattaaac atcagcgcct ttgggctacc
    22081 aaaggcggct tatctcaatc ccgcctggta cgatgctatt acgccgcagg atgcatggac
    22141 ctgggcattt ctcgatctca tcggccaggt gaaattcctc acgctttttg cgctgctgtt
    22201 tggtgctggc ctgcaaatgt tgctgccccg tggcagacgc tggatccagt cgcggttaac
    22261 gctgttagtc ttgttgggct ttattcacgg tttattgttc tgggacggcg atattctgct
    22321 ggcttacggg ctggtggggc ttggcgtttt gctgttattg gggctgattt ccgacagcca
    22381 gaccagccgc gcctggacgc cggatgcatc ggctattttg tatgaaaaat actggaagct
    22441 tcacggcggc gttgaagcga tcagtaatcg tgccgatggt gttggcaaca gtttactggc
    22501 actgggcgca cagtatggct ggcaactggc agggatgatg ctcattggtg cggcgctgat
    22561 gcgcagcggc tggctgaaag ggcagttcag cttacgtcac tatcgtcgta ctggttttgt
    22621 gctggtggcg attggggtga ccattaacct tcctgccatc gccctgcaat ggcagctgga
    22681 ctgggcgtat cgctggtgtg ccttcttact tcaaatgccg cgggaactga gtgcgccgtt
    22741 tcaggcgatt ggctatgcgt cgctgtttta tggcttctgg ccgcaattga gccgctttaa
    22801 gctggtgctt gcgatcgcct gcgtcggacg gatggcgctg accaactatc tattgcaaac
    22861 gctgatttgt accacgcttt tttaccacct cggtttgttt atgcattttg accgcctgga
    22921 gctgctggcg tttgttattc cggtatggct ggcgaatatc ctcttctctg ttatctggct
    22981 gcgttacttc cgccaggggc cggtggaatg gctctggcgt cagttaactt tgcgtgctgc
    23041 cggaccggca atatctaaaa catcaagata acgatctgga ttacattcgt taacaaaacg
    23101 gctgtaaccg tttccattgc tgtgactcga ttcacgaagt cctgtattca gtgctgacaa
    23161 aatagccgcc agcaagcagt catttactgc aatctcataa caggtagtga atatgatcac
    23221 cattcgtgat gtagcgcgtc aggctggcgt ctctgtggca acggtttccc gggtgctcaa
    23281 taacagcacg ctggtcagtg ccgacacgcg tgaagcagta atgaaagccg tgagtgagct
    23341 ggattatcgg ccaaacgcca atgcccaggc gctggcaact caggttagcg acaccattgg
    23401 cgtggtggtg atggacgttt ctgatgcgtt tttcggcgcg ctggtaaaag cggtggatct
    23461 ggtcgcccag cagcatcaga aatacgtgct aatcggcaat agctatcatg aagcggaaaa
    23521 agagcgtcac gccattgagg tgttaattcg ccagcgttgt aatgcgttga ttgttcactc
    23581 aaaagcattg agtgacgatg aactggcgca atttatggat aacattcccg gtatggtgtt
    23641 aatcaaccgc gttgtgccgg ggtacgccca tcgttgcgtt tgcctggata atctcagcgg
    23701 tgcccgaatg gcgacgcgca tgttgctgaa taacggtcat caacgtattg gttatctttc
    23761 ttccagccac ggcattgaag atgacgccat gcgtaaagca ggctggatga gtgcgttgaa
    23821 agagcaggat attattccgc cggaaagctg gattggcact ggtacgccgg acatgccggg
    23881 cggtgaggcg gcgatggttg aactgctggg gcgcaatcta caacttaccg ctgtatttgc
    23941 ttataacgac aatatggccg ctggcgcact gacagcatta aaagataatg gcattgcgat
    24001 tccgttacat ctctcaatca tcggtttcga tgatattccc atcgcccgtt acaccgaccc
    24061 gcaattaacg accgtgcgtt atcccattgc ttcaatggct aaattagcca ccgaactggc
    24121 cttgcagggg gcagcaggca atattgatcc tcgtgccagc cactgtttta tgccgacgtt
    24181 agtgcgtcgc cattctgtcg caacgcgcca gaatgcggcg gcgatcacta actcaacaaa
    24241 tcaggcgatg taaccgcttt caatctgtga gtgatttcac agtatcttaa caatgtgata
    24301 gctatgattg caccgtttta atgttgtaac ccgtatgtaa cagtgaataa tcactttcac
    24361 cgaggtaata gcgtcataac aacaattaaa gccgttttct ggagcgttac cgggcatgga
    24421 agaacgaatt ttaaaaagtg agcttcggcg ttcagtaaca cttcattaac tctactgccc
    24481 cgccgagcat ttatctcaag cactaccctg cataagaaaa accggagata ccatgaataa
    24541 gaaggtgtta accctgtctg ctgtgatggc cagcatgtta ttcggtgccg ctgcacacgc
    24601 tgctgatact cgcattggtg taacaatcta taagtacgac gataacttta tgtctgtagt
    24661 gcgcaaggct attgagcaag atgcgaaagc cgcgccagat gttcagctgc tgatgaatga
    24721 ttctcagaat gaccagtcca agcagaacga tcagatcgac gtattgctgg cgaaaggggt
    24781 gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc
    24841 gcgtgggcaa aacgtgccgg tggttttctt caacaaagaa ccgtctcgta aggcgctgga
    24901 tagctacgac aaagcctact acgttggcac tgactccaaa gagtccggca ttattcaggg
    24961 cgatttgatt gctaaacact gggcggcgaa tcagggttgg gatctgaaca aagacggtca
    25021 gattcagttc gtactgctga aaggtgaacc gggccatccg gatgcagaag cacgtaccac
    25081 ttacgtgatt aaagaattga acgataaagg catcaaaact gaacagttac agttagatac
    25141 cgcaatgtgg gacaccgctc aggcgaaaga taagatggac gcctggctgt ctggcccgaa
    25201 cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga
    25261 agcactgaaa gcacacaaca agtccagcat tccggtgttt ggcgtcgatg cgctgccaga
    25321 agcgctggcg ctggtgaaat ccggtgcact ggcgggcaca gtactgaacg atgctaacaa
    25381 ccaggcgaaa gcgacctttg atctggcgaa aaacctggcc gatggcaaag gtgcggctga
    25441 tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta ccttatgttg gcgtagataa
    25501 agacaacttg gctgaattca gcaagaaata agactgattg ggcgcagtcg tttactgcgc
    25561 ccttattgta tggccaacaa ggtaaaatta tatggtcagc tcaacgactc cgtcttccgg
    25621 ggaatacttg ttggaaatga gcggtatcaa caagtccttt cctggtgtta aggcacttga
    25681 taacgttaat ttaaaagtcc ggccacattc tatccatgca ttaatggggg aaaacggtgc
    25741 aggaaaatcg acattattaa aatgcctgtt tggtatttat caaaaagact ccggcaccat
    25801 tttattccag ggtaaagaga tcgatttcca ttccgccaaa gaggcactgg aaaatggtat
    25861 ttcgatggta caccaggagt taaacctggt attacaacgt tcggtgatgg ataacatgtg
    25921 gctggggcga tatcccacca aaggcatgtt tgtcgatcag gacaaaatgt accgcgaaac
    25981 caaagcgatt tttgatgaac tggatattga tatcgatccg cgtgcgcgcg tcggcacatt
    26041 atccgtttcg caaatgcaga tgatcgaaat cgccaaagcg ttttcctata acgcgaaaat
    26101 tgtgattatg gatgaaccga cttcttcgtt aaccgaaaaa gaggtcaatc atctgttcac
    26161 tattattcgt aaattaaaag agcgcggctg cggtattgtt tatatctcgc ataaaatgga
    26221 agaaatcttc cagttatgtg atgaagttac cgtattgcgc gacggtcagt ggatcgccac
    26281 cgaaccgctg gcaggactga cgatggacaa gatcatcgcc atgatggttg ggcgttctct
    26341 taatcagcgt tttcctgaca aagaaaacaa gccgggcgaa gtcatcctcg aggtgcgtaa
    26401 cctaacgtca ctgcgccagc cgtcgattcg cgatgtctcg tttgatctgc ataaagggga
    26461 gatcctcggt attgccgggc tggtgggggc gaaacgtacc gatattgttg agacgttatt
    26521 tggtattcgc gagaaatcgg ctggcaccat tacgttgcac ggcaaacaga tcaataacca
    26581 taatgccaac gaagccataa accacggatt tgcactggta actgaggagc gccgctcaac
    26641 gggaatttat gcctatctgg atattggttt taactcgtta atttccaata ttcgcaacta
    26701 caaaaataaa gttggtttac tggataactc gcggatgaaa agcgataccc agtgggtgat
    26761 tgattcgatg cgggtaaaaa cgccgggtca tcggacgcaa attggttcgc tctccggtgg
    26821 taatcagcaa aaggtgatta ttggtcgctg gctactaacg caaccagaaa tattaatgct
    26881 cgatgaaccg acgcgcggta ttgatgtcgg ggcgaagttt gaaatttatc agttaattgc
    26941 cgaactggcg aagaaaggca aggggattat tattatctcc tctgaaatgc ctgagttgtt
    27001 agggataaca gaccgtattc tggtcatgag caatggtctc gtttccggaa ttgtcgatac
    27061 aaaaacaaca acgcaaaacg aaattctgcg tcttgcgtct ttgcaccttt aagattaggg
    27121 gcttcccatg agtgcgttaa ataagaaaag ttttcttact tacctgaaag agggcggtat
    27181 ttacgtcgtt cttttagttt tgctggcgat tattattttc caggacccaa catttttaag
    27241 tctgttgaac ttaagtaata ttctcaccca gtcatcggtg cgtattatta tcgcgctcgg
    27301 tgtggcaggg ttaattgtca cccaggggac cgatctttct gctggtcgtc aggtagggct
    27361 ggcggcagtg gtggctgcga cattattgca gtccatggat aacgccaaca aagtgttccc
    27421 ggaaatggcg acgatgccga ttgcgctggt tattctgatt gtctgtgcca ttggtgcggt
    27481 gatcggtttg atcaacggtc tgattatcgc ttatctcaac gtgacgccgt tcattaccac
    27541 gctcggcacg atgatcatcg tctatggcat caactcgctc tattacgact ttgtcggggc
    27601 gtcgccaatt tctggttttg acagtggctt ctctaccttt gctcagggct ttgtcgcgct
    27661 ggggagtttc cgtctctctt acatcacctt ctacgcgttg attgcggtgg cgttcgtctg
    27721 ggtgttgtgg aacaaaaccc gcttcggtaa gaacattttt gccattggcg gtaacccgga
    27781 agcggcaaaa atatctggtg tcaacgtcgg cctgaacctg ctgatgatct acgcgttgtc
    27841 tggcgtgttc tatgcctttg gcgggatgtt agaagccgga cgtatcggct ctgccaccaa
    27901 caacctcggc tttatgtatg agctggatgc tatcgcggcg tgcgtggtag gcggtgtatc
    27961 gttcagcggc ggtgtgggga cggtgattgg cgtggtgacc ggggtaatta tttttaccgt
    28021 catcaactat ggcctgacgt atatcggcgt aaacccatac tggcagtaca tcatcaaagg
    28081 ggcgattatt atcttcgccg tagcgctgga ttcactgaaa tacgcgcgta agaagtgatg
    28141 tgattgcgga tattgccgga tgcgtctctg gttttacaac gttaccgggt gttctttctc
    28201 gcctttctta aacttcactt ccccgagctc aatacaaccc accgggcaga cgtgaccaca
    28261 aagcagacaa cccacacatt tctcggtatt acaatgcggt gtgcgggttt tctcgctcca
    28321 ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa cagcgtccac agccaacaca
    28381 tttgtcaaga ttgatacggg gatagacaat ataactgcgg tctaaatctt ccgccgggac
    28441 aatattgtta ttcgccagac ctaccatctc ctgcaacgaa tcaaatccct gatcggcgag
    28501 ataatgcgat aacccgctcg ccatatcttc tacgatgcga tagccgtact gcataatgcc
    28561 ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac tccgccgcat cttcccaggt
    28621 ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt tctggatggg tgcgcatttg
    28681 ctgaatgaag cgcagggcga tcggttttac cgctttaccg gaatatccgg aaatactcga
    28741 tttaccgtta acgatcggca taccgatttt ctgattaaga tcgatattgg tgatggattt
    28801 aacggtgtta attgccgcaa tgccatctgc gccgccgcgc ttcgccgcca gcgccacttc
    28861 gcacatatca ccgatattcg gcgtcatctt cgccagcatt ggcagcgtgg aaccccgttt
    28921 cactgcccga caatattttt ctaccagctc cgggctttgc ccgacatcgc tacccatcgc
    28981 atgagaagtc atttgcggac aggagaagtt acactcgatc atatccgcgc cagctgctcc
    29041 cattgctgct cattttcccc catgatcgaa gcgatcaata ctttgtccgg gtaatcttcc
    29101 ttcagccgac gcagggcggt cagattctct tccaacggat gttcagcaat ctgctccata
    29161 tttttgaagc cgataaaacc ggtatcttct ttcaccagat gatcaaaacg cggcgagact
    29221 tcgttggcga taaaaaagcc gatcgtttta aacaccacac cgccccaacc tgtgtcgtag
    29281 gctttggcac acatctcata gcagttgcct accggcgaag aagagaggca gaacgggttg
    29341 ggaaagttca cgccgcaaaa agtaatcgaa agatctttcg ttaacatgag caagctccct
    29401 ctaaatagtg atgaatcgcc tcggcggctt ctttcccggt tttcacggca tagaccacgg
    29461 ttttgtcacc ctcaacaata tcgccagcag caaagacttg cggatcgcgg atctgataat
    29521 tttgtgtttt gatggtgtta cgctgctgct ctaactccgc aaaggcatcc agtctggcat
    29581 gctgaccgac ggcgagaata attttatctg ccgccatcgt cagttcgcct gataaccgta
    29641 catgcttaaa cgtgacttta ttgccttcca cggctactgg cgtgaatcca tcgatgatcg
    29701 aaacgcccag ctcccgggcg ctggcaaact ctttttcgct tgccggaaac tcatctaact
    29761 cttcacgtgc tacgcaagtt accgcctgac agccgagaac tttcagcgtg ctggctacgt
    29821 ccatcgcgac atcaccgccg ccgataatta atgcgctttg tggaatgctg atatcgcctt
    29881 gcgcctgtcg tacacgttgc aggaagtcga cggcaatctc aacgtcgcta tgctcaaaca
    29941 gcggtagccc ggaaccgctt gataacccca cggtgaccag taccgcgcgg ttttccgctt
    30001 tcagttgctc cagggtgagt gttttaccga tttcgttatt gcacttaatg gtcacgccca
    30061 ttttttcaat acgggcgatc tctgcatcca gcactgattg cggtaaccgg aattgcggaa
    30121 taccgttacg caaccagcca ccggggtgcg cttctttctc ataaatcgtg acgtcataac
    30181 cctggtttgt cagtgtcaca ctggcctgca atccggcagg acctgcgcca ataatcgcga
    30241 ctttgccgag cgttttagta ccgggctgat aaatttccat cccggtttgt tgttcaaaat
    30301 cagtaacaaa acgctgtaag cggccgatat caatgggagc atcaacaccg gcacgggtac
    30361 aaccgctttg gcataatttc tccgtcgggc acactctggc acaaacggca ccgagggcat
    30421 tattttcgcg aattgtctcg gcagcgcctt taaaattacg aaagtagatt gagcgaataa
    30481 atttccccgg atcggtctgt gccgggcaag cctgactaca gggagcgtcg tgacataata
    30541 aacagcgaga ggcttcttta atcgccagta acggcgtaaa agccggagtg agttcatcca
    30601 gataattttg ttgcggcatg tgatcttcct taatgctatg agtaattatg gcgaacgcta
    30661 aataaatatt gcgtgctgat attaaggcta tgcgcgtggg gaaatttaag ctgtgaaagg
    30721 attcacaaat agctgcggtt cagaatgtta tatccgcagc ttattgagtg aagtaataat
    30781 tattctcaat aattaacaga aaagagaaaa tattaacgat agaaaataag ataaacaatc
    30841 atgccgatag ataaaattcc gaagccaata tatgccacca tataagcgaa gaagagtccg
    30901 ggattttttg ctcgccagac atacaccggt tccggtgcat ttttaacccg tttatcaatc
    30961 gcgcctgcgc gccagccttt ccatgcttcg cggaaacaaa atatcgggat aaggaaaaaa
    31021 acggcaacga caaaaaactg ctgtacatcc ataactactt tccttgtttc ttttgtaatt
    31081 caagtaactg ttcgggtgta acggcgggat acccctgcgc atcttccggc acctggtgca
    31141 tagccggaat agggaccagc ggccctaaaa aacgcggttc acgtttaaaa atataaaggt
    31201 cagccagcgc accgaaacgg gcggcaaatt cgcgaatacg tactgacagc atatctttcg
    31261 gtgacggtac ggcataacac tgagcctgaa tccccatatg cagcgcaata aataatgctc
    31321 gctcacagtg gaaacgttgg gtgataataa tgaaatcatt agtatcgaaa actttgcgtg
    31381 tacgcacgat ggaatccagc gtacgaaagc ctgcgtaatc gagaacaata tctgatgggt
    31441 cgacaccagc agcgattaaa tctttgcgca tggtcatcgg ctcgttataa ctttgcaatg
    31501 cgttatcgcc gctcagtaat agataattta ccttaccgct gttataggca ttaatcgctc
    31561 cttgaatgcg gtagcgataa tactgattaa taacgccggt acgataatat tttgctgtgc
    31621 cgagcaccac gccgacctgg cggtagggga gatcctgcaa ttcgtcgtag atataaggcg
    31681 cggttttcca gctcatccag cgatcgaggc cgagcacagt caacagcagc aagccgatca
    31741 ggactaacag gctgaggaac acgcgcttta acatgcagga gactcgagct aaggaaaatt
    31801 tccatcaggc tactttaccc gctggttttg cgcaagaaac ggcaaactgt ttgccgggaa
    31861 tttcgacacc aggagagggc tggcaagccg gacaaggcgt tcacgccgca tccggcacca
    31921 ggcttaagcg agaagcacgc ggtcgatact gtgacagccg agagctttca gcgttgcgga
    31981 ggtggcatcc cactgaatca acggcgcatc ggctttttct gccagaaccg cgcgctggat
    32041 atccgggtaa tcataaccct tgagattcaa cagaattaac gccccctgca acggcggcag
    32101 agtcgggttg aatgcggcgt tttcagcgta gctgccgctg aaaatacgac cgtctttaca
    32161 ttccagcgcg acaccgcttg gtgacttact gtaaggcatg tgcgaacggt ttgccgctgc
    32221 aatcgccgcc tgagaaagcg catcaccagt cagcgcatag ccgtgatcct gctcgtccat
    32281 cagcagcgtt ttaatctcca gatctttcgg cccaaaggca tccggcagat agtcacgcag
    32341 cgcgtgtgcc tcgcggcccg gcaaatgaat acgcagatcc agaccgctgt tcagttcatt
    32401 cataaactga cggcagtgac cacaaggcgt atagttaacg gtgatggctg caagcgcttt
    32461 ttcaccactc aaccaagcgt ggctgatcgc gctttgttcg gcatgaacgg tttgctgcat
    32521 tgttgcacca ataaattcca tattggcacc aaaataccag gttccgctca caccgcgcgc
    32581 aattgcgcca acattaaaat tcgacaatgg cgtacgcgca caggccgccg ccagcggaag
    32641 tagtgcgaat gccagcgcgt cttcgtccag tcccgttgcg ctcttcagcg atgagacttg
    32701 ctccccggtc aacaaagcgg ggaagtactt gtctgccaga ataggttcca gtgcagattg
    32761 caagttatcc gcaagttggg caaaagcggt ttgaaaacgt ggatgcatgg cgttgcctca
    32821 taatcatgta atgggataac agtttacgaa cccgttatgg ctttatatgt gatctgaatc
    32881 tcattaatta tgcaacacgt gaaacttaca tcaaaaatgc gcgacgtatc gcaaatttta
    32941 gcccattact gccagaataa tcgggaaaag gaacggtgcg atcagcgaag taattatccc
    33001 gcataacacc agcgctagcg aactaaatgc accttcctga taatccagct cggcgcagcg
    33061 cgccgtaccg agggcgtgcg aggcagtccc cattgccaga ccgcgcgcag ctttggtacg
    33121 aatacgcatc gcattaagca atgtatggcc aaataccgcg ccgaggatgc cgacgaaaat
    33181 cacgcaaact gcgctgattg ccggaatacc gccaatactg ccgccaaccg ccattgcaat
    33241 aggcgtggtg actgatttcg gcaggattga cgcggcgatt tccggtgaag cgcccatcaa
    33301 caatgccacg gaagtccccg tcaccatcgc aaccacgctg ccgataaaac agatggtgat
    33361 gatcgatttc cagcgcgcgc ggatctggtg tagctgctca tataaaggat aggccagcgc
    33421 gacgaccgcc ggttgcagca gatcgtttag cacttcgcta cctttaaagt aactgtcgta
    33481 agagatgccg gtcagcatta aaaaaggaat gatcaccacc attgctacca gcaacgggtt
    33541 aagcagggga aacttatacc gtgccgccag tttgcgggcg gcaaaaaaga caatcaacgt
    33601 cagcggtaat gaccaccaga tattcgccat cattcttctg atcctttctg acctacgact
    33661 ttacgttcac cgtgtaccag ttgtgaactc cagctcacca ccagaaaaac caccagcgta
    33721 ctgacagcac aggaaaccac taccgggcca aactgcgcgc ggagcaaatc aaaatattgc
    33781 atgacgccta cgccaatcgg cacaaacaat agcgccatat agcgaatcag tacgtagcac
    33841 cccggattga cccatttcgc cggaagaatt tgcaaggcca gcaggacaaa caggatcagc
    33901 atcccgatga tgctgcccgg aatggttacc ggtagcaggg aagcaatgaa aatgcctgca
    33961 tacaggcagg cataaatcag gacgaaagcg cgtaaatatt gccagataat gttcagtgtc
    34021 ttactcataa tcaacacccc agatgagatg gattcatcat acaattaatt cgtaaaacgt
    34081 gctaccgatc acattaagaa atcagcgctt attttttcgc tacccggtat tacgcaatga
    34141 gcagcgaact caggcataca tcaagcatag agagggattg cagtttgcag caatccctct
    34201 ttgcagacct ttaatccgct gaccataggc gctaacatag cgcctcattt ttttgcgggt
    34261 gatgatatgc gtgtgttact ggcaccgatg gagggagtgc ttgactctct ggtgcgtgaa
    34321 ttgctgaccg aagttaacga ctacgatctg tgcatcaccg agtttgtccg cgtggtggat
    34381 caactgctgc cggtaaaagt ctttcatcgc atttgccctg agctacaaaa cgccagccgg
    34441 acaccatctg gtacgctggt gcgcgtgcag ttgttaggtc agttcccaca atggctggca
    34501 gagaacgccg cccgtgcggt ggagttaggt ttctggggcg tggatctcaa ttgcggctgc
    34561 ccgtcgaaaa cggttaacgg tagcggcggc ggggcgacgt tactcaaaga tcctgaactc
    34621 atctaccagg gggcaaaagc gatgcgtgaa gctgtaccgg cgcatttgcc tgtcagcgtg
    34681 aaagtgcgtc tgggctggga cagcggtgag aagaaatttg aaatcgccga tgcggttcaa
    34741 caggctggcg ctacggagct ggtggtgcat gggcggacga aagagcaggg ttaccgcgcg
    34801 gagcatattg actggcaggc gattggcgag attcgccagc ggctgaatat tccggtgatt
    34861 gccaacggtg aaatctggga ctggcagagc gcgcaacaat gcatggcgat cagcggctgc
    34921 gacgcagtga tgattggtcg cggggcgctc aatattccca acctgagccg ggtggtaaaa
    34981 tataacgaac cgcgaatgcc gtggccggag gtggttgctt tgctgcaaaa atatacccgt
    35041 ctggaaaagc agggcgatac cgggttatat cacgttgcgc ggattaaaca gtggttgagt
    35101 tatttgcgta aagaatacga tgaagcaacg gaattatttc agcatgttcg ggtgttgaat
    35161 aactcccctg atattgcaag ggctattcag gcaattgata tcgagaaact ctaaaaacca
    35221 tagtgatgtt tttgacaatg tgttagctct ttatcaatag gccgcacggt tgtttggtgg
    35281 cgtcaaaatc gccgaggcgt tccctgaagg ccggggcagc ccacatggat gtgggctgag
    35341 ggcgcgtttt acagggatgt tacctcgcgc ccgacccggt agccgtaagg gataagccga
    35401 gggcaccgcg cagcggcgat tttgttcgcc agagcccggg ggggcagggg gcggcggcga
    35461 ttggtcgccc cctgcgcgct ccttgcgcca gtgattatat gaagcaatgc acatgacagg
    35521 agcgcaacag gatgagagac tgaaatattc tcgttatttt cggatctgat gacattaatt
    35581 aattttaaaa tatcatctta aatacgccag tcaccaccag tagcccaatc aggaaaataa
    35641 tcaatacagc ccagagtata attttcattg gcgttccttt atttgtgagt gctgaataag
    35701 tataggaaaa aacgaagccc tttgccgtta tccataaacg attaagataa atcggaaaat
    35761 atcatttact tagtctatat ttttctgggt atgtcgtttt attgctactg atgtattgcc
    35821 acgcgaaact aaaaaatata tcgcaacaca cggtaatctc cgcgcaccgt ttcttacctg
    35881 attattcccc catgaatcgt gattcctttt atccagccat cgcctgtttt ccgctgttac
    35941 tgatgctggc cgggtgtgcg cctatgcatg aaacccgcca ggcgttaagc cagcaaacgc
    36001 ccgctgcaca agttgacacc gcattaccca cggcgctgaa aaatggttgg ccagacagcc
    36061 aatggtggct ggagtatcac gataatcagc tcacttcatt aattaacaat gcactacaga
    36121 acgcaccgga tatgcaggtg gctgaacagc gcatttagct agctgaagcc caggcgaagg
    36181 cagttgccac tcaggatggt ccgcagatcg acttttcggc ggatatggag cggcaaaaaa
    36241 tgtcggcaga agggctaatg gggccgtttg ctctgaacga tccggccgca ggtacgaccg
    36301 gtccgtggta caccaacgga acttttggct taacggcggg ctggcatctc gatatctggg
    36361 gaaagaatcg ggcggaggtt actgcccgcc tgggtacggt taaagcacgg gcggcggaac
    36421 gcgagcaaac ccgccaattg ctggctggca gcgtagcccg cctgtactgg gagtggcaaa
    36481 cccaggcggc gttaaacacg gtcttgcagc aaatagaaaa agagcagaac accattatcg
    36541 cgaccgatcg ccagctatat cagaacggga ttacttcttc agttgacggt gtggaaaccg
    36601 atattaatgc cagcaaaacc cggcagcagc tcaacgatgt cgcggggaaa atgaaaatta
    36661 ttgaggcacg gttaaatgca ctcacaaatc accagactaa gtcattgaag cttaaaccgg
    36721 tcgcgttgcc gaaagtggca agccagcttc ctgatgaact ggggtactcc ttactggccc
    36781 ggcgggcaga tttgcaggcg gcgcactggt acgttgagtc atcgttaagc actattgatg
    36841 cggcaaaagc ggcattttat cctgacatca acctgatggc cttcctgcaa caggatgcgt
    36901 tgcacttaag cgatctgttc cgtcattccg cgcagcaaat gggcgttacg gcaggcctga
    36961 cgctacctat tttcgatagt ggtcgtctta acgccaatct cgatatcgca aaagccgaaa
    37021 gcaacttgtc tatcgccagc tacaacaaag cggtggttga agcagtgaat gacgtggcgc
    37081 gggcagccag tcaggttcag acactggcgg agaaaaacca gcatcaggcg caaattgagc
    37141 gcgatgcctt gcgtgtggta ggtcttgcgc aggcccgctt taacgcgggc atcattgctg
    37201 gttcccgcgt cagcgaagcc agaatcccgg cgctgcgtga gcgggccaat ggcctgttat
    37261 tgcaagggca gtggctggat gcctccattc gactcaccgg tgcgctgggc gggggttaca
    37321 aacgctgatg atatgcgcct tctatactta acgtttattc agcgttaagt ggagaactcg
    37381 atggcatagg tcgcgataat taccgcttcc gattcgggga tcggcaaaga gtgcgcgtta
    37441 ttactggcgc agcaggggtt tgatattggc attacctggc actcagatga agaaggggca
    37501 aaagataccg cgcgtgaggt ggttacccac ggcgtacgtg cggagatcgt gcaactggat
    37561 ctcggcaatc tgccagaagg tgcgcaggcg ctggagacac tcatccagcg atttgggcgt
    37621 attgatgtgc tggtgaataa tgcgggtgca atgaccaaag cgccgtttct tgatatggct
    37681 tttgatgagt ggcgcaagat ttttaccgtt gatgttgatg gtgcattttt atgctcgcaa
    37741 attgcggctc gtcagatggt gaaataaggg cagggcggtc gcatcatcaa cattacgtcg
    37801 gtacatgaac atacgccgct gccggatgcc agcgcctaca cagccgctaa acatgcgctc
    37861 ggcgggttaa ccaaaacgat ggcgctggag ttggtcaggc ataagatttt ggtgaacgca
    37921 gtcgcgcctg gggcgatagc cacgccaatg aacagcatgg atgatagcga cgtgaagccc
    37981 gacgcggagc cttcgattcc tctacggcgt tttggcgcaa cgcatgagat tgccagcctg
    38041 gtggtgtggc tttgttcgga gggcgcaaat tacacgacag ggcagtcgtt gatagtggat
    38101 ggcggcttta tgttggcgaa tccgcagttc aacccagaat agcgcagtga ggtgcgtgga
    38161 tatttcaatg ggttaggcca gataagacac cagcgtcgca tcaggcatgg aacacccggt
    38221 gccggatgtg gagtgaatgc ttatctgtgg caactgcttt acgcctgatt atccggcttt
    38281 ttcttcccgc gccgtttcag ccaccagcgc acgcccacca ccagaaccac gaccagaatc
    38341 aaccagaccc agtgcttcaa atgctggtcg agattgtgca accacggcgc aatcacctga
    38401 ccaccagcgt aaccaatagt ggtaaaaatc aacgcccagg caaatgcgcc gagaatattc
    38461 agcggcagaa agattttcgg cggcagctgg ctggcaccaa tcagcgtcgg gccaatcacc
    38521 cgaaagccat acataaagcg cgtaccaatg acaaacagat acggatggcg ttggataagt
    38581 ttctgtgccc gctcaatttt atcctgatgt ttcgagaaac ggcgtaacag cttgccgcca
    38641 aaccgccgcc cgcacagata gagcacctgg tcgccaatca tgccaccaag cgccacagaa
    38701 agtaccacca gtgggaactt taatagcccc tgatgtgccg caacgcctcc cagcaaagtc
    38761 acggtttcac cttccgccag gctaccgata accagcgcgg cataaccata ttgtgagatg
    38821 agtgtattga gatccatagg ccagtttgtc tccctttggt tgcctgtaag catacaccct
    38881 gtgaatcatt cacgctgaaa aaggacatac atcagacggt gcaaattaca tggatgaaca
    38941 aaaaataatc acaagctggc ttatacttga actgtcaggt tcatgaactc acaacaaggg
    39001 ggcggtatga gccatgtctg gggactgttt tcccatcccg atcgtgaaat gcaggtgatt
    39061 aatcgcgaaa acgaaacgat ttctcaccat tacacccacc acgttttgct gatggcggcg
    39121 attccggtga tttgcgcctt cattggcact acacagattg gctggaattt tggcgatggc
    39181 actatcctga agttatcctg gtttaccgga ctggcgctgg ctgtcttgtt ttacggcgtt
    39241 atgctggctg gggttgcggt gatggggcgg gtcatctggt ggatggcgcg caattacccg
    39301 cagcgtccgt ctcttgcgca ctgcatggtc ttcgcgggct acgttgcaac tccgctgttt
    39361 ttaagcggtc tggtggcgct ttatccactg gtctggttgt gtgcgctggt cggcacggtg
    39421 gcgctgtttt acaccggcta cctgctttac cttggtattc cgagcttcct gaatatcaac
    39481 aaagaggaag gccttagctt ctccagttcg acgctggcga ttggcgtgct ggtactggaa
    39541 gtgctgctca ccctaaccgt tatactatgg ggctacggtt atcgtttgtt ctagaaagtt
    39601 ctttgacgtt gcattgctgg cgtaaatgtc agcaatgcag catttactca cgattctcct
    39661 ttgacgatcg cactttttgc tcgttatgat ggcgtcgcta gcctcagtaa atccttactc
    39721 cggcggtgcg caacccgtgc gcgtgaacca ctatctgaat gctcatcatg ccgaaatttc
    39781 gagtttcttt atttagcttg gccctgatgc tggctgtgcc ttttgcaccg caggccgttg
    39841 cgaaaacggc agccgctacc accgcttcac aaccggaaat tgcctccggt agcgcgatga
    39901 ttgttgatct gaataccaac aaagtgatct attcgaacca cccggatctg gtgcgtccga
    39961 ttgcgtctat cagcaaatta atgaccgcga tggttgtgct ggatgcacga ctgccgctgg
    40021 atgaaaaact aaaagtggat atcagccaga cgccggagat gaaaggggtc tattcgcgcg
    40081 tacgactgaa tagcgaaatc agccgtaaag atatgctgtt gctggcgctg atgtcttcag
    40141 aaaaccgcgc ggcggcaagc cttgcgcacc attatcccgg tggttacaaa gcctttatta
    40201 aggcaatgaa tgcgaaagcg aaatcgctcg gaatgaacaa cacgcgcttt gttgaaccta
    40261 ccggattgtc ggtgcataac gtttcaactg cccgtgactt aaccaaactg ctcattgcca
    40321 gcaaacaata tccgttgatc gggcagttaa gtactacccg ggaagatatg gcaaccttct
    40381 ctaacccgac gtatacgctg ccgttccgca atactaatca tctggtgtat cgcgataact
    40441 ggaatattca gttaaccaaa accggcttta ccaatgcggc gggccattgt ctggtgatgc
    40501 gtacggttat caataataaa ccggtggcgc tggtagtgat ggacgcgttt ggcaaatata
    40561 cccattttgc cgatgccagc cgcctgcgta cctggattga aaccggtaaa gtgatgcctg
    40621 tgccggcagc agcgttgagc tataaaaaac aaaaagccgc ccaaatggcg gcggcggggc
    40681 agacggcaca gaacgattaa gtgaattcgg atggcgatac tctgccatcc gtaattttta
    40741 ctccacttcc tgccagtttt tccgtttact ggttttaccg atccccggat tcatgctatt
    40801 ggtcggatca ttctcgcgat agaacttctg caacgtctcc ggtgctttat acaaatgacc
    40861 gacgttatgc tcggcagggt actgcgcgcc gcgctgctgt agcagttcca gcatctgttc
    40921 ttttaacgca tgcacatcca cgcctttttt cactatgtaa tcctgatgga agacatagca
    40981 cataaaatgg ccgtaataga gcttgtgcac cagctggctg tcgatctccg gcggtaaatg
    41041 ctcataccac tcggtgtcgt tacgccgcag agcgatatcc agtgccagaa tgtcttcgac
    41101 ttcatcggaa tgcaccgcct gataacgaat tgctgcgccc gcagcggcga aacggtgtaa
    41161 aaacgctttg ctaccttcct ccggcgtaca gacaaagaaa tcgccctcgg cctgtttgaa
    41221 ataatccacc agccacgatt tggcttcacc gacgccatcg cctgccattt ttaacagcag
    41281 atgatgctcg tatttatcgc gccagttttt catgcgcggc ggtaaatggc tggggaacag
    41341 gtgaccgaat ttttgcattg cacggtcagt aaaatgcgga cggaagaatt tcactttctc
    41401 cagcatcgca tcggtgcgtc ccttgagatt aaagaagaac ggcatcttgt cggtgcctaa
    41461 cttatcaatc atcaggaagg tgtctttgcc gtatttttcc gcaatatcgt agatatcccg
    41521 gtgcatatat tccccggcaa ccggcagatt ttcgaagtta gccagaatat gacggcggat
    41581 ttcggtcagc acttccggct ggttggtgcc gatataaaac acctgctgat ttttttccgc
    41641 ttcgaaggta tcaagacgta ccgcaaagac ggccagcttc ccggcgcaac cagaagattc
    41701 aaataaccga tccggatcgg cgttataacg tgcgggcgtg tcggcttcaa tatcacgaac
    41761 gcggtggaca tagtcataat cgtgggcgtg acgaccatcg tgacgcacat cgtcatcttt
    41821 gatgcgatcg tcatccagct tgctaaggat ctgctccggc gtttcgccca gatcaatccc
    41881 cagatggttc accagcgtca gtttgccatc ttcatctata cgcgcgaata acgacatttc
    41941 ggtatacgcc gggccacgtt gcaccagcga gccgccggag ttgttacaaa taccgccgat
    42001 gaccgatgcg cctatacacg atgatccaat caccgaatgc ggttcgcgtc ccagcggttt
    42061 gagggctttt tccagcgaat agagcgtggt gcccggatag gccagcacct gttcgccctt
    42121 gccgagaacg tgcagcttgt cgagacgcag ggtgctgatg atcacgatat cgcgatcata
    42181 gtcgttaccg tttggcgtcg atccttcggt caggcctgta ttggcagcct gcatcagaat
    42241 aattttgtcg gcggtgacgc aggctttcag cacccgccac aattccagta gtgagccagg
    42301 gaaaacgaca gccagcgcgt cgccctgacc agaacggaag cccttgcgat agcgggccgt
    42361 ttttgcggga tcggtgagca ggtgtgaatg accgaccaga cgggcaagtt cattcaaaaa
    42421 ggctttatta tcagttgttg tcatggaaga cattttccac tccttgtggt ggcgaaaaaa
    42481 tatcacaaac tagaatagcg ccttgtatga caattcagcg agtgaatatt ttgaaaattc
    42541 agacaataaa ctgagcattc tttttctctc tccatcatgc ttatggcaaa ctacggtttt
    42601 tgcaccattt tcgctgccac gtcgggcaac aaaggaagaa aaatccatat gaaatggcta
    42661 tgttcagtag gaatcgcggt gagtctggtc ctgcagccag cactggcgga tgatttattc
    42721 ggtaaccatc cattaacgcc tgaagcgcgg gatgcgttcg tcaccgatct gctcaagaaa
    42781 atgacggttg atgagaaaat tggtcagctg cgcttaatca gcgtaggccc ggataatccg
    42841 aaagaggcga tccgcgagat gatcaaagac ggtcaggttg gggcgatttt caacaccgta
    42901 acccgtcagg atatccgcgc catgcaggat caggtgatgg aattaagccg cctgaaaatt
    42961 cctcttttct ttgcttacga cgtgctgcac ggtcagcgca cggtgttccc gattagcctc
    43021 ggtctggcct cgtcttttaa cctcgatgcg gtgaaaacgg tcgggcgtgt ctctgcttat
    43081 gaagcggcag atgatggcct gaatatgacc tgggcaccga tggtcgatgt ctcgcgcgat
    43141 ccgcgctggg gacgtgcttc cgaaggtttt ggcgaagata cgtatctcac ctcaataatg
    43201 ggtaaaacca tggtggaagc gatgcagggt aaaagcccgg cagatcgtta ctcggtgatg
    43261 accagcgtca aacactttgc cgcatacggc gcggtagaag gcggtaaaga gtacaacacc
    43321 gtcgatatga gtccgcagcg cctgtttaat gattatatgc cgccgtacaa agcggggctg
    43381 gacgcaggca gcggcgcggt gatggtggcg ctgaactcgc tgaacggcac gccagccacc
    43441 tccgattcct ggctgctgaa agatgttctg cgcgaccagt ggggctttaa aggcatcacc
    43501 gtttccgatc acggtgcaat caaagagctg attaaacatg gcacggcggc agacccggaa
    43561 gatgcggtgc gcgtggcgct gaaatccgga atcaacatga gtatgagcga cgagtactac
    43621 tcgaagtatc tgcctgggtt gattaaatcc ggcaaagtga ccatggcaga gctggacgat
    43681 gctgcccgcc atgtactgaa cgttaaatat gatatggggt tgtttaacga cccatacagc
    43741 catttggggc cgaaagagtc tgacccggtg gataccaatg ccgaaagccg cctgcaccgt
    43801 aaagaagcgc gtgaagtggc gcgcgaaagc ctggtgttgc tgaaaaaccg tctcgaaacg
    43861 ttaccgctga aaaaatcggc caccattgcg gtggttgggc cactggcgga cagtaaacgt
    43921 gacgtgatgg gcagctggtc cgcagccggt gttgccgatc aatccgtgac cgtactgacc
    43981 gggattaaaa attccgtcgg tgaaaacggt aaagtgctgt atgccaaagg ggcgaacgtt
    44041 accagtgaca aaggcattat cgatttcctg aatcagtatg aagaagcggt caaagtcgat
    44101 ccgcgttcgc cgcaagagat gattgatgaa gcggtgcaga cggcgaaaca atctgatgtg
    44161 gtggtggctg tagtcggtga agcacagggg atggcgcacg aagcctccag ccggaccgat
    44221 atcactattc cgcaaggcca acgtgacttg attgcggcgc tgaaagccac cggtaaaccg
    44281 ctggtgctgg tgctgatgaa cgggcgtccg ctggcgctgg tgaaagaaga tcagcaggct
    44341 gatgcgattc tggaaacctg gtttgcgggg actgaaggcg gtaatgcaat tgccgatgta
    44401 ttgtttggcg attacaaccc gtccggcaag ctgccaattt ccttcccgcg ttctgtcggg
    44461 cagatcccgg tgtactacag ccatctgaac accggtcgtc cgtataatgc cgacaagccg
    44521 aacaaatata cttcgcgtta ttttgatgaa gctaacggag cgctttatcc gttcggctat
    44581 ggtctgagct ataccacttt caccgtctct gatgtgaaac tttctgcgcc gaccatgaag
    44641 cgtgacggca aagtgacggc cagcgtgcag gtgacgaaca ccggtaagcg cgagggggcc
    44701 acggtagtgc agatgtactt gcaggatgtg acggcttcca tgagtcgccc tgtgaaacag
    44761 ctgaaaggct ttgagaaaat caccctgaaa ccgggcgaaa ctcagactgt cagcttcccg
    44821 atcgatattg aggcgctgaa gttctggaat caacagatga aatatgacgc cgagcctggc
    44881 aagttcaatg tatttatcgg cactgattcc gcacgcgtta agaaaggcga gtttgagttg
    44941 ctgtaatgct ttagtaaggg cgcgacgttt gcgccctttg cctgatgttc atatcttaac
    45001 ttgctgaatt ttcgcccttt gtaggccgga taaggcgctc gcgccgcatc cggcaggaaa
    45061 cgccggaccg aatatgcctc ttaagtattt atctgattct gcaactgaat ccttccgctc
    45121 aggctaactc tgtcattatc aactatgctt ttctcttaat tcgctgaaaa caacagtaaa
    45181 agaatgagga aagcatcatg ccactcttaa agctctgggc aggttcactg gttatgttgg
    45241 cagccgtgag cctgccgcta caggcggctt cccccgttaa agtcggttca aaaatcgata
    45301 ccgaaggcgc gctgctcggc aacatcatct tgcaggtact cgaaagccac ggcgtaccga
    45361 cggtgaataa agtgcaactc ggaacgactc ctgtggggcg cggggcgatt acttccgatg
    45421 aactggatat ctatccggaa tataccggca atggcgcttt cttctttaaa gatgaaaacg
    45481 atgcagcgtg gaaaaatgcc gggcagggct acgagaaagt caaaaagctc gatgcggagc
    45541 acaacaagtt aatctggctg acgcccgcgc ctgcaaataa cacctggact atcgccgtgc
    45601 gtcaggatgt ggcagagaaa aacaaactca cttcgcttgc cgacctgagt cgttatctgc
    45661 aagagggcgg caccttcaaa ctggcagcct cggcagagtt tatcgaacgc gccgatgcgt
    45721 tacccacgtt tgaaaaagcc tatggcttta agctcggtca ggatcagttg ctgtcactgg
    45781 ctggtggcga cacggcggtg acgattaaag ccgctgccca gcaaacctct ggcgttaatg
    45841 ccgcaatggc ttacggcact gacggtccgg tcgcggcgct ggggctgcaa accttaagcg
    45901 atccgcaagg cgtgcaacct atctacgcgc ctgcaccagt ggtgcgtgaa tcggtgttga
    45961 aggagtatcc gcaaatggca cagtggctac agccagtctt cgccagcctc gatgcaaaaa
    46021 cattgcagca actgaatgcc agcattgcag tggaaggact ggatgccaaa aaagtggctg
    46081 ccgactacct gaaacaaaaa gggtgggcga agtaatttcc cgtgacttat ctacgtatta
    46141 atcctgttct ggcgctgctg ttgttgctga cggcaatcgc agcggcgctg ccgtttatca
    46201 gttacgcgcc taatcgttta gtctcgggtg aagggcttca tctctggcaa ctgtggccgc
    46261 aaacgatctg gatgctggtg ggcgttggtt gcgcctggct gacagcctgt tttgtccccg
    46321 ctaaaaaagg cagcattttt gcactcattc tggcgcaatt cgtcttcgta ttgttggtgt
    46381 ggggagctgg aaaggcggcg acccaactgg cgcaaaatgg cagtgcgctg gcgcgtacca
    46441 gcctcggcag aggtttctgg ctggctgcgg cgctggcatt gctggcctgt agcgatgcca
    46501 tccgccgaat ctccacgcat ccgctgtggc gctggttgtt gcatatgcag attgccatta
    46561 ttccgctgtg gttgctgtac tccggcacgc ttaacgatct ctcactaatg aaagaatacg
    46621 ccaaccgtca ggatgtgttt gacgacgcgc tggcacaaca tctgacgttg ctgtttggtg
    46681 cggtgctgcc tgcgttagtg attggtgtgc cgttgggcat ctggtgctac ttttccactg
    46741 cgcggcaggg ggcaattttt tctctgctca atgtcattca gaccgtgcct tcggtggcgc
    46801 tctttggcct gttgattgcg ccgcttgccg cgctggttac ggcctttccg tggctgggga
    46861 agctcggcat agcagggacc ggaatgacac ccgcactgat tgcgctggtg ctctatgcct
    46921 tgctgccgct ggtgcgaggc gtggtagtcg gtttgaacca gatcccgcgc gatgtgctgg
    46981 agagcgccag agcgatgggg atgagcgggg cgcagcgttt cctgcatgtt cagttaccgc
    47041 tggcgttacc ggtatttctg cgcagcctgc gggtggtgat ggtgcaaact gtaggtatgg
    47101 cggtgattgc ggcgttaatc ggcgcaggcg gttttggtgc gctggttttc caggggctgc
    47161 taagcagcgc cattgattta gtgttgctgg gggtgatccc ggtaattgtt ctggcggtgc
    47221 ttaccgacgc gctgttcgat ttgcttatcg cactgctgaa ggtgaaacgt aatgattgaa
    47281 tttagccatg tcagcaaact gttcggcgca caaaaagccg ttaacgatct caatctcaat
    47341 tttcaggaag ggagtttttc ggtgctgatt ggcacatctg gctccggcaa atccaccacc
    47401 ctgaaaatga ttaaccgcct ggtggagcat gacagcggag tgatccgctt tgccggagaa
    47461 gaaattcgct cgctgccagt actggagttg cgccgccgga tgggctatgc cattcaatct
    47521 attggcctgt tcccccactg gagcgtggcg caaaacatcg ctaccgtgcc gcaattacaa
    47581 aaatggtcgc gggcgcggat tgacgatcgt atcgacgaat taatggcgct actggggctg
    47641 gagtcaaatt tgcgtgagcg ttatccgcat cagctttccg gtggtcagca gcaacgtgtg
    47701 ggagtggcgc gtgcactggc tgccgatccg caagtcttac tgatggatga accttttggc
    47761 gcactggacc cggtaacgcg cggcgcgttg caacaagaga tgacgcgcat tcaccgtttg
    47821 ctggggcgca ctattgtgct ggtcactcat gatattgatg aggcgctacg gctggcagaa
    47881 catctggtat tgatggatca cggtgaagtg gtgcagcagg ggaatccgct gacgatgctg
    47941 actcgtccgg cgaatgattt tgtccgccag ttttttggac gtagtgaact gggggtgcac
    48001 ctgctttcgt tacgtagcgt ggcggattac gtgcgtcgcg aagaacgggc agaaggtgag
    48061 gcactggcag aagagatgac gctacgcgat gcgctctccc tgtttgtcgc gcggggatgc
    48121 gaggtgctgc cggtggtgaa cacgcagggc cagccttgcg gcacgctgca ttttcaggat
    48181 ctgctggtgg aggcgtaagc gtatgaagat gttgcgcgat ccgctgttct ggctcattgc
    48241 tctttttgtg gcgctgattt tctggctgcc ttacagccag ccgctgtttg ctgccttgtt
    48301 cccacaactg ccacgacccg tttatcagca agaaagtttt gcagctctgg cactggctca
    48361 tttctggctg gtgggaattt cgagtttgtt tgcggtgatc attggcacag gtgccggaat
    48421 tgctgttact cgcccgtggg gcgcggaatt tcgcccactg gtggaaacta ttgccgccgt
    48481 tggacagact tttccgcctg tcgcagtgct ggcgattgcc gttccggtga tcggctttgg
    48541 tctgcaacca gcgattatcg ccttgatcct ttacggtgtg ctgcccatcc tgcaggtgac
    48601 acttgccggg ctgggagcga ttgatcccag cgtgacagaa gttgcgaaag gtatgggaat
    48661 gagtcgaggt cagcgactgc gtaaggtcga gctaccgctg gcggctccgg tgattctggc
    48721 gggcgtgcga acttcggtga ttatcaacat tggtacggcg acgatcgcct caacggtagg
    48781 ggccagtacg ctgggtacgc ccatcatcat cgggcttagc ggatttaata ccgcgtatgt
    48841 gatccagggg gcgttactgg tggcactggc ggcgatcatc gcagaccgcc tgtttgaaag
    48901 gctggtgcag gcgcttagcc agcacgcaaa ataaaggtat aacctgcgag catgacgcca
    48961 ccaattccgc ctaacgccat aaacaggaac agggcgatga ccccaatttt agctatgcgc
    49021 ataatgcact ccttatgtta acgaaagg
//
LOCUS       DAJTPG010000018        48323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTPG010000018 DAJTPG010000000
VERSION     DAJTPG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            177..470
                     /locus_tag="OO230_001106"
     CDS             177..470
                     /locus_tag="OO230_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5690947.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(511..1701)
                     /gene="nepI"
                     /locus_tag="OO230_001107"
     CDS             complement(511..1701)
                     /gene="nepI"
                     /locus_tag="OO230_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5690948.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1912..2364)
                     /locus_tag="OO230_001108"
     CDS             complement(1912..2364)
                     /locus_tag="OO230_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5690949.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="OO230_001109"
                     /gene_synonym="yicO"
     CDS             complement(2417..3751)
                     /gene="adeQ"
                     /locus_tag="OO230_001109"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5690950.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3926..5692
                     /gene="adeD"
                     /locus_tag="OO230_001110"
     CDS             3926..5692
                     /gene="adeD"
                     /locus_tag="OO230_001110"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5690951.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="OO230_001111"
     CDS             complement(5738..7129)
                     /gene="uhpT"
                     /locus_tag="OO230_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5690952.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="OO230_001112"
     CDS             complement(7267..8586)
                     /gene="uhpC"
                     /locus_tag="OO230_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5690953.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="OO230_001113"
     CDS             complement(8596..10098)
                     /gene="uhpB"
                     /locus_tag="OO230_001113"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5690954.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="OO230_001114"
     CDS             complement(10098..10688)
                     /gene="uhpA"
                     /locus_tag="OO230_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5690955.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="OO230_001115"
     CDS             complement(10763..11053)
                     /gene="ilvN"
                     /locus_tag="OO230_001115"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5690956.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="OO230_001116"
     CDS             complement(11057..12745)
                     /gene="ilvB"
                     /locus_tag="OO230_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5690957.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="OO230_001117"
     CDS             complement(12851..12949)
                     /gene="ivbL"
                     /locus_tag="OO230_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5690958.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13514..13603
                     /gene="tisB"
                     /locus_tag="OO230_001118"
     CDS             13514..13603
                     /gene="tisB"
                     /locus_tag="OO230_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5690959.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13883..15067
                     /gene="emrD"
                     /locus_tag="OO230_001119"
     CDS             13883..15067
                     /gene="emrD"
                     /locus_tag="OO230_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5690960.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15075..15572)
                     /locus_tag="OO230_001120"
     CDS             complement(15075..15572)
                     /locus_tag="OO230_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5690961.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15569..15931)
                     /locus_tag="OO230_001121"
     CDS             complement(15569..15931)
                     /locus_tag="OO230_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5690962.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15921..16268)
                     /locus_tag="OO230_001122"
     CDS             complement(15921..16268)
                     /locus_tag="OO230_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5690963.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16377..16790
                     /locus_tag="OO230_001123"
     CDS             16377..16790
                     /locus_tag="OO230_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690964.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16837..18279)
                     /locus_tag="OO230_001124"
                     /pseudo
     CDS             complement(16837..18279)
                     /locus_tag="OO230_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18314..20029)
                     /locus_tag="OO230_001125"
     CDS             complement(18314..20029)
                     /locus_tag="OO230_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5690965.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVIITFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20196..21089
                     /locus_tag="OO230_001126"
     CDS             20196..21089
                     /locus_tag="OO230_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5690966.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21086..21900)
                     /locus_tag="OO230_001127"
                     /pseudo
     CDS             complement(21086..21900)
                     /locus_tag="OO230_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21900..23516)
                     /locus_tag="OO230_001128"
     CDS             complement(21900..23516)
                     /locus_tag="OO230_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5690967.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23811..24485
                     /locus_tag="OO230_001129"
     CDS             23811..24485
                     /locus_tag="OO230_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5690968.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24524..26185)
                     /locus_tag="OO230_001130"
     CDS             complement(24524..26185)
                     /locus_tag="OO230_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5690969.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26381..26809)
                     /gene="ibpB"
                     /locus_tag="OO230_001131"
     CDS             complement(26381..26809)
                     /gene="ibpB"
                     /locus_tag="OO230_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5690970.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26921..27334)
                     /gene="ibpA"
                     /locus_tag="OO230_001132"
     CDS             complement(26921..27334)
                     /gene="ibpA"
                     /locus_tag="OO230_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5690971.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27328..27400)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27639..27971
                     /locus_tag="OO230_001133"
     CDS             27639..27971
                     /locus_tag="OO230_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5690972.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27973..29217)
                     /locus_tag="OO230_001134"
     CDS             complement(27973..29217)
                     /locus_tag="OO230_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5690973.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTNTWTPDSQWLVFDVRPSG
                     ASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYD
                     FHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQ
                     LDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEE
                     GWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRG
                     VVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPR
                     KLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVF
                     SPDGQWLAWMEGGQLWITETDR"
     gene            29318..30418
                     /gene="cbrA"
                     /locus_tag="OO230_001135"
     CDS             29318..30418
                     /gene="cbrA"
                     /locus_tag="OO230_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5690974.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30379..31716)
                     /locus_tag="OO230_001136"
     CDS             complement(30379..31716)
                     /locus_tag="OO230_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5690975.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALCFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31791..32939)
                     /gene="dgoD"
                     /locus_tag="OO230_001137"
                     /pseudo
     CDS             complement(31791..32939)
                     /gene="dgoD"
                     /locus_tag="OO230_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32936..33553)
                     /gene="dgoA"
                     /locus_tag="OO230_001138"
     CDS             complement(32936..33553)
                     /gene="dgoA"
                     /locus_tag="OO230_001138"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5690976.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33537..34415)
                     /gene="dgoK"
                     /locus_tag="OO230_001139"
     CDS             complement(33537..34415)
                     /gene="dgoK"
                     /locus_tag="OO230_001139"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5690977.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34412..35101)
                     /gene="dgoR"
                     /locus_tag="OO230_001140"
     CDS             complement(34412..35101)
                     /gene="dgoR"
                     /locus_tag="OO230_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5690978.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35379..36035
                     /locus_tag="OO230_001141"
     CDS             35379..36035
                     /locus_tag="OO230_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690979.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36081..36893)
                     /gene="yidA"
                     /locus_tag="OO230_001142"
     CDS             complement(36081..36893)
                     /gene="yidA"
                     /locus_tag="OO230_001142"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5690980.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37007..37405)
                     /locus_tag="OO230_001143"
     CDS             complement(37007..37405)
                     /locus_tag="OO230_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5690981.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37645..40059)
                     /gene="gyrB"
                     /locus_tag="OO230_001144"
     CDS             complement(37645..40059)
                     /gene="gyrB"
                     /locus_tag="OO230_001144"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5690982.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40088..41161)
                     /gene="recF"
                     /locus_tag="OO230_001145"
     CDS             complement(40088..41161)
                     /gene="recF"
                     /locus_tag="OO230_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5690983.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41161..42261)
                     /gene="dnaN"
                     /locus_tag="OO230_001146"
     CDS             complement(41161..42261)
                     /gene="dnaN"
                     /locus_tag="OO230_001146"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5690984.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42266..43669)
                     /gene="dnaA"
                     /locus_tag="OO230_001147"
     CDS             complement(42266..43669)
                     /gene="dnaA"
                     /locus_tag="OO230_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5690985.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43966..44046
                     /locus_tag="OO230_001148"
     CDS             43966..44046
                     /locus_tag="OO230_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5690986.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44276..44416
                     /gene="rpmH"
                     /locus_tag="OO230_001149"
     CDS             44276..44416
                     /gene="rpmH"
                     /locus_tag="OO230_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5690987.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44433..44792
                     /gene="rnpA"
                     /locus_tag="OO230_001150"
     CDS             44433..44792
                     /gene="rnpA"
                     /locus_tag="OO230_001150"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5690988.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44756..45013
                     /gene="yidD"
                     /locus_tag="OO230_001151"
     CDS             44756..45013
                     /gene="yidD"
                     /locus_tag="OO230_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5690989.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45016..46662
                     /gene="yidC"
                     /locus_tag="OO230_001152"
     CDS             45016..46662
                     /gene="yidC"
                     /locus_tag="OO230_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5690990.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46768..48132
                     /gene="mnmE"
                     /locus_tag="OO230_001153"
     CDS             46768..48132
                     /gene="mnmE"
                     /locus_tag="OO230_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5690991.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11985 a        13061 c        12217 g        11060 t
ORIGIN      
        1 cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa aagtggaata
       61 taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg atgagagcga
      121 tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga
      181 aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg
      241 agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac
      301 gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt
      361 cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa cgtaataatc
      421 agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac
      481 aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt
      541 aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc
      601 aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa gctgaataac
      661 cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg tgatccacgt
      721 tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca cgccggtagc
      781 aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca gtattaacgg
      841 cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa gcgacgtacc
      901 aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca cgccgaatcc
      961 cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc cggcgaaaga
     1021 catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc ggaaagtatt
     1081 ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa tccagaaaat
     1141 acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga taagctcgcc
     1201 taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac
     1261 cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg acgccgacat
     1321 cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa gcaaactgaa
     1381 tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa acaaaataac
     1441 aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg caaacattgc
     1501 cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg
     1561 ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac aggcgacaca
     1621 aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt
     1681 ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa aagccgacca
     1741 ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg tgttgcttga
     1801 gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg tgaacgtctt
     1861 atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt ttcaatgcaa
     1921 ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa aagcattaat
     1981 atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga cctgagcatc
     2041 ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca gcaggttctg
     2101 ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg cgttttgcag
     2161 gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg actttcccat
     2221 ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc tgagacggcg
     2281 aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca ctaccgccag
     2341 cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca tgatatctgg
     2401 aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga gctgcgacca
     2461 ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg atgcagtacg
     2521 acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg
     2581 taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag
     2641 tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga accatcgcca
     2701 ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca gccgttagcc
     2761 ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta acagacgagg
     2821 taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc ttattcatat
     2881 tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca attaatgttc
     2941 ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg
     3001 ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat
     3061 agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag
     3121 aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta
     3181 aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc gtgtctttat
     3241 tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat ccgattccac
     3301 tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac cggatacgaa
     3361 acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc ccggtctgcc
     3421 aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc cccattgccg
     3481 gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa
     3541 tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg
     3601 ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca atcaattctg
     3661 tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat
     3721 tactcacgta atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc
     3781 cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc
     3841 acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa
     3901 ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat ttcatcacat
     3961 tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta
     4021 tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt ccggcccaat
     4081 tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata ctccggcttt
     4141 gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca
     4201 tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct
     4261 gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc
     4321 ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg
     4381 cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct
     4441 cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt
     4501 aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc tgacgctgga
     4561 cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg cgggtattga
     4621 aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc
     4681 gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac cgttgatcaa
     4741 cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc
     4801 ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct
     4861 gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc tgaatcacct
     4921 cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt
     4981 cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct tacaggcgga
     5041 agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg cccgccagcc
     5101 agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc gggtcattga
     5161 cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg aaaatggttt
     5221 tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac ggctggctcc
     5281 ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg cgacagtcag
     5341 ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg cgctggcggt
     5401 caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc aagtacaaag
     5461 tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt cgctggcgga
     5521 acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt
     5581 tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg
     5641 gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat aataaaaaaa
     5701 gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc aactgctgaa
     5761 tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc atcaggcaga
     5821 tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccga
     5881 ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca
     5941 ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc
     6001 caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga
     6061 gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc gtggcgataa
     6121 tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg tcagagagcc
     6181 agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg
     6241 ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac tggtcgatac
     6301 caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc actttgtttt
     6361 tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttgt
     6421 cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca taagattccg
     6481 gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata atcgacggga
     6541 agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac agcgccacac
     6601 ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg aatgtcccgc
     6661 gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtactct
     6721 ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc
     6781 ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg aacggcagga
     6841 attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc acgccataag
     6901 tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaaa
     6961 tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc aggtagccga
     7021 taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga
     7081 gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg ggttactcct
     7141 gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca ggcctgagat
     7201 agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt gaaaaggtga
     7261 gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga
     7321 aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg tatcgagcac
     7381 tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc cgacaaaccc
     7441 cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac caattaacat
     7501 ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca tcacgtagct
     7561 ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg cgaaaatcaa
     7621 attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg ctaccagcgc
     7681 accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga ccagatcgac
     7741 gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc ggaccacata
     7801 gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca catatttggt
     7861 gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt ccagcgcgtc
     7921 gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc gtagccgcca
     7981 gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc cggcacgcca
     8041 gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc cgacgttatg
     8101 cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc aggccgttaa
     8161 cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca cccagagcac
     8221 ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc ccgtggcgat
     8281 aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa
     8341 cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat cgctacggct
     8401 gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc gcgtgaagta
     8461 aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt agcgataacg
     8521 ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt tcagaaacgg
     8581 caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac gcgtgccgtg
     8641 cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca ttccggtgag
     8701 gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt caatcaccag
     8761 catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat cggcatgttt
     8821 cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct ggttttcgct
     8881 taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc cttccagctc
     8941 catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca gctggcgcgg
     9001 acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca gttgttcgat
     9061 gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa caatgcccgc
     9121 ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac gcgccacgtc
     9181 gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat tgcgcgccag
     9241 ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc ccagtaacaa
     9301 ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat cgcgccaggt
     9361 ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc cttgccagcc
     9421 atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa agcgcgacag
     9481 ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca agtaccagac
     9541 cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga gcggcagcca
     9601 ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg tcagaccgcc
     9661 agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc agggcagcga
     9721 ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc gccagtcacg
     9781 ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac taccgatcat
     9841 caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca gcagccactc
     9901 cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc ccagacgcag
     9961 accaaacgga aataacagca ccgccatatc agggcgctca accagatgca ggctgatact
    10021 ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg taattaagcg
    10081 ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc tacgtcgtta
    10141 ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt cggtgacaag
    10201 cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc cgccacctgg
    10261 cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc aatatccggc
    10321 gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc atccgggcta
    10381 cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac cagcgcagga
    10441 ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct tagcagctcc
    10501 agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc gcgccccggc
    10561 agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa atcaggttcc
    10621 agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat aagggcaacg
    10681 gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg
    10741 atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta aacatcgtcg
    10801 gatcggactg attacgctgc actttcacga catcttccag cttagcgatt tggcttatca
    10861 tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg tcgctgtcct
    10921 gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa
    10981 cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg ttgttatgag
    11041 ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct ggcggcacca
    11101 tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg ccagggcgat
    11161 tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca caggtttcga
    11221 ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg gcggcaaaaa
    11281 cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct tcgttgttca
    11341 tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc tgaatattca
    11401 tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc gccagcgccg
    11461 cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag gtcagccact
    11521 ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga cccacatcgg
    11581 tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg ccgtaatggc
    11641 ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa tccgctacca
    11701 actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc gccagcacgt
    11761 catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc tctgcgcgat
    11821 cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg ccaatcgccc
    11881 ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa atatagttgg
    11941 tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct tttggcagca
    12001 tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc agttcacgca
    12061 cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt ttggcagcgt
    12121 taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca acggcttttt
    12181 ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc ttaggaatgt
    12241 ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct
    12301 gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg atagagatgc
    12361 cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga acctgaccag
    12421 tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc accaggttag
    12481 tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg cgcgccattc
    12541 cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg atttgcgtgc
    12601 tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc gtcacaatct
    12661 taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag cgcttacgcg
    12721 tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg actttccggg
    12781 aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga atcgtgtgtt
    12841 gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg accactgcgg
    12901 cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt ggggaccttg
    12961 ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga caatggaaaa
    13021 tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg cagataacaa
    13081 aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc aaagcacact
    13141 gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa
    13201 ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg tccaaatatt
    13261 actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt tatgcggggg
    13321 ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta
    13381 accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt gagataccgc
    13441 gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc
    13501 acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac tcattgttgc
    13561 agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat tcaagtcgca
    13621 ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg gagtcggatg
    13681 cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat tgccgctttt
    13741 gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca
    13801 tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat
    13861 ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt tattgatgtt
    13921 ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga
    13981 tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct
    14041 gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg tcggtcgccg
    14101 accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac
    14161 ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg
    14221 cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt tgcgccatgc
    14281 taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc taatcggcgg
    14341 tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg ttctttgtgc
    14401 tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg atgcaccgcg
    14461 cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt
    14521 gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct ccggcgtgct
    14581 gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat
    14641 tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct tctcaacgtt
    14701 gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga tccccgactg
    14761 gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct ttttcggtgc
    14821 cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct tcctggcggg
    14881 cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct
    14941 ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga ccttaatggg
    15001 attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc aggggcagcc
    15061 cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt
    15121 atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc ctgatagccc
    15181 gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg
    15241 cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg
    15301 gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca ctcaaacagg
    15361 gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg
    15421 tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg tttgaaattg
    15481 tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt atgcctgtcc
    15541 tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt
    15601 gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa
    15661 atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc
    15721 gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca atcgaaatcc
    15781 agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt
    15841 agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc cctgggtcgg
    15901 caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac gatcaccgcc
    15961 acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag gtcctctttc
    16021 agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc taacccgccg
    16081 gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc gaaatctggc
    16141 gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg gatccacgcc
    16201 agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa
    16261 attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg ttttttttaa
    16321 gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga ggtcagatgg
    16381 gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc atcgctttaa
    16441 tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca acgcttgaaa
    16501 gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga ataaaaggcg
    16561 aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc ctcgatcaaa
    16621 gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt atgcgtaagg
    16681 aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg tgcagcattt
    16741 tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa gcagcctgaa
    16801 taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt ttttctcctc
    16861 caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg gcgaatagcc
    16921 atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt tacgccacgg
    16981 acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa
    17041 ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca ggttatgcat
    17101 ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt tgagcaccag
    17161 tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc
    17221 aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg caaggatatt
    17281 ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg tcggcagtaa
    17341 atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg aacggatgat
    17401 cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca gcttatgtgc
    17461 gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac gttgctctgg
    17521 cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc aggcaaaata
    17581 gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct gcgcccataa
    17641 gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa gttcgtagta
    17701 aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat gcggctcatc
    17761 atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca gaaaatctac
    17821 cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt tcgcctgtaa
    17881 atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg tcagttcgct
    17941 aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac actcgccagt
    18001 gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt gatagccggc
    18061 atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt tgttggtcca
    18121 cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag gcgtacaaac
    18181 cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac tatcaatatt
    18241 ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct gggtatcggt
    18301 caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg gctgggtcgt
    18361 gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg atgaaataac
    18421 tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg acatagacgc
    18481 caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac ggtttcatgt
    18541 ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata aaaccgatca
    18601 ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg aaatggaagt
    18661 cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc atcgccactt
    18721 ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg gtaaccagcg
    18781 gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc ggcgcgttcg
    18841 cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat ttgcgcccga
    18901 cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa atacccatac
    18961 tgaataatgt actggcgcta ttcagaaagc cgttgaaggt aatgatcacc gcaccaaata
    19021 acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc agcgtcgggt
    19081 aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac cctggcagca
    19141 ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc ttttgccctt
    19201 ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta caccagtaaa
    19261 aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga tcggtcgggc
    19321 caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg ccctgcataa
    19381 agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg ccgataacca
    19441 gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg atcaccgcat
    19501 acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca ccgtgagaaa
    19561 tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg gagtagagaa
    19621 caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg ataatacgcg
    19681 tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg cgcttcagat
    19741 agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc acttcccagc
    19801 ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt tgttccgtgg
    19861 aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa cgtccggcaa
    19921 gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag gtgatcaccg
    19981 ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc tcatagccct
    20041 tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg tcgggtcagg
    20101 cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc tgttaagttc
    20161 aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg caaagttcag
    20221 atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat gtgatctcca
    20281 gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac cgggtgatct
    20341 ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg tataccgtac
    20401 aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat agcaactgta
    20461 ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta gtctgggaac
    20521 agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt cagcaaagcg
    20581 ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag ttaataactt
    20641 caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt atttatcagt
    20701 ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa attgaaaaat
    20761 taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat atggctgcca
    20821 cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc ggcaagacgc
    20881 cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta aagcaacaag
    20941 gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt catttcagca
    21001 aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat acggaccagc
    21061 acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct ttgttggcct
    21121 ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt ttcgacaacg
    21181 taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg accagttttt
    21241 ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg ccgaccacca
    21301 gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg
    21361 cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt tcgcattcgg
    21421 gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt tgagcatcta
    21481 ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc caatggaacg
    21541 catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac cgtggcgcag
    21601 tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt gcgccatgac
    21661 aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg caatgtccgg
    21721 cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc gcgcgccatc
    21781 gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa
    21841 cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga atttggtcat
    21901 tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat taagctatcg
    21961 agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc atcgccacta
    22021 cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt ttgtgacatg
    22081 tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat attgccgacc
    22141 ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg ctctgcagcg
    22201 gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac ttccgcatct
    22261 tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac cacgaagtac
    22321 agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc gtggttgctg
    22381 aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc catgttgccc
    22441 accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc gtgtaccgca
    22501 aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat tcccaccagc
    22561 atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt ttccggtgcg
    22621 gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga gttaccgtgc
    22681 agggcaaaac cgccttccag gaacaacgat ttcagtggct cggcgctcag actgaactct
    22741 tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg accaaagata
    22801 aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg
    22861 taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc
    22921 atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat caccaggaag
    22981 gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta
    23041 tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc gccgataatg
    23101 ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc caccgcgtcc
    23161 tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt gatgaaatag
    23221 ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc ttgcttagca
    23281 aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac cgtccaaccg
    23341 ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt cagtgattcc
    23401 ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc ggcgaaggga
    23461 aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact gagcatttcc
    23521 acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg cagacctgga
    23581 gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc gtgatcgctt
    23641 tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga catctgtgag
    23701 cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat cgattaagca
    23761 gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag atgatctaca
    23821 aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg ctaaacagcc
    23881 ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg actatccgta
    23941 aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc agcggcactt
    24001 acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg gtggaggtgt
    24061 taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc
    24121 ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac ttctcccgtc
    24181 gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg ccggtaaaac
    24241 tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat attgaacaag
    24301 agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc gccgatagac
    24361 tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc acctcgttgt
    24421 catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat gccagcgaat
    24481 actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg atactccaga
    24541 agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac ggatagacag
    24601 tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca aacgccagcg
    24661 ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg
    24721 ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc agggcaccat
    24781 aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc ccaccagatt
    24841 tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg aggttggcac
    24901 ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg atcaacgcca
    24961 taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct ggcacaaaga
    25021 cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc agcatctgaa
    25081 cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg
    25141 gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca ctggcgacca
    25201 gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca
    25261 ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc tcgacgcgca
    25321 aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc agttgcgcat
    25381 tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg ataatggtat
    25441 ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata attttgtcgc
    25501 cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc tcaacgcgaa
    25561 tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc tgctgagctt
    25621 ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac aaaatcccgc
    25681 aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc atttccattg
    25741 gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta ttggttaccg
    25801 caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta tgcaggatgg
    25861 cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg
    25921 agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta taaacgaaca
    25981 ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct
    26041 gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata cctaatccta
    26101 tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa gccagaatac
    26161 tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt ttaattgcta
    26221 cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat cacgggaaag
    26281 aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa
    26341 aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt aacgcgggac
    26401 gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga attaaatcaa
    26461 tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca gccagcgtaa
    26521 aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc tcttcttttg
    26581 gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc tctaaatctt
    26641 cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa
    26701 tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg gccagtttgt
    26761 caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata gtcatttctc
    26821 cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc cctgacggcg
    26881 agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt tcgatacggc
    26941 gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa ccatttacca
    27001 ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca aagttgcgtt
    27061 cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga gcacctttca
    27121 ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa ccagccacag
    27181 cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga gggtagccgc
    27241 cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat ccaatagcag
    27301 aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga aatcagcgag
    27361 aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca ggcgcgtcct
    27421 catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat cagcggactt
    27481 ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc
    27541 gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact
    27601 catcaggcca acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag
    27661 cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg atgtcccata
    27721 ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg attggcgacg
    27781 atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtaa
    27841 tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg cgctcgcgcg
    27901 ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg gccagaatgc
    27961 ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt ccatccacgc
    28021 cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat tggcgtggtt
    28081 ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc gattatccag
    28141 cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa tatccgtttt
    28201 gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca gttgcacaat
    28261 gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca
    28321 gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg ttaaacgtcg
    28381 ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta acggcgcatc
    28441 acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata gctccggcac
    28501 tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca
    28561 tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg gcgtggtttt
    28621 actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct tttgtacgtt
    28681 gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg gatcgagttg
    28741 atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc taaagacatg
    28801 cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat ccattgcatc
    28861 gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga agtcgtaatg
    28921 ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg acttcggatg
    28981 aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct cgacctcgcc
    29041 tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag aaggacgcac
    29101 gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat tggtattggt
    29161 attggtattg gtattggtga gcaggtgatt acggggagca aaggttatct gtttcatggc
    29221 gcggtcctga tacaaaacgg ttcccgccat catacttcac aaggacttca ctttcagaca
    29281 atagtctttt gccatcgtga tgaatgtgag tgaaaagatg gaacatttcg acgtggcgat
    29341 tattggcctc ggcccggcag ggtcggcgtt ggcacgaaag ttagccggca aaatgcaggt
    29401 gatcgcgctg gataaaaagc accagtgcgg tactgaaggt ttcagcaaac cttgcggcgg
    29461 tctgctggca ccggacgcgc agcgatcttt tattcgcgat ggactgacgc ttcctgtcga
    29521 tgtgatcgcc aatccacaga ttttcagcgt caaaactgtc gacgtcgccg catcgctcac
    29581 gcgtaactac cagcgaagct atatcaatat taatcgccat gctttcgact tgtggatgaa
    29641 atcgctgatc cccgccagcg ttgaggttta tcacgatagc ctgtgccgaa aaatctggcg
    29701 tgaggatgat aaatggcatg tcatttttcg tgcagacggc tgggagcaac atattaccgc
    29761 ccgctatctg gtcggtgccg atggcgcaaa ctcgatggtg cggcgacatc tctacccgga
    29821 tcatcaaatc cgtaaatatg tcgctatcca gcagtggttc gcagagaaac atccggtgcc
    29881 gttctactcc tgcatctttg ataatgcgat aactgactgt tactcatgga gtatcagcaa
    29941 agatggttat tttatctttg gcggtgccta tccaatgaaa gacggtcaga cgcgtttcac
    30001 gacgctgaaa gagaaaatga gcgcctttca gttccagttt ggcaaagcgg tgaaaagtga
    30061 gaaatgcacg gtgttgtttc cctcacgctg gcaggatttt gtctgtggta aggacaacgc
    30121 ctttctgatt ggtgaagcgg cgggatttat cagcgccagc tcgctggagg ggattagcta
    30181 tgcgctggat agcgcagaga ttctgcgtgc ggtgttcctg aaacagccgg agaagagcaa
    30241 cgccgcttac tggcgcgcca cacacaaact gcgtttaaaa ctcttcggca agatagtaaa
    30301 aagccgatgc ctgaccgcac cggctttaag aaagtggatt atgcgcagtg gtgtggcgca
    30361 tattccacag ttgaaagatt atccaacgcg cttcacatca cccaccagca ggatgtaaga
    30421 gagcgcgcca atcaacgcga cggcggagat ataaaccagt gcaggcgcga aaccgtaacc
    30481 ctgcgccagg taccccacca ccagcggaac ggtgatgccg cccagaccac cggcgaagtt
    30541 aaacacgccg ccggttaaac caatcaggcg catcggtgcc agagaagaga ccagcgacca
    30601 ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc atcaggcaca taatcatcat
    30661 cggatcgttg gtgtagttag cgcccataat gcaggtggag atcagcaagc cgcagataat
    30721 cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat ccgcgaccca
    30781 gccagagagc aggacgccga caaacgccgc gaggaatggc accgtggtca taaagcctgc
    30841 tttcagcgcc gtgattcctt tttcctgggt taaatagttc gggaaccagg ttaagaaaaa
    30901 acacagtgca gaagccaccg caaattgccc aagataaacg ccgatcagtt tacgatggaa
    30961 caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc tctttcttca ccggcgcatc
    31021 accatccacc agaccgccgc catcacgaat gtaatccagt tcagctttgc tgataccttt
    31081 ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc gaccagataa tgccgatacc
    31141 accagtgaca atgaacaccc agtgccagct caacatctcc tgaatccaga tcagcagcgg
    31201 agtcaaaaac gccagaccga caaactgacc agacgtataa aaaccaacgg cggaagcgcg
    31261 ttcatgttcc gggaaccagc tggtcaccat ccggttattg gtcgggaaag cgggcgcttc
    31321 gaaaataccg gttatcgcac gcagaccaat taatgacatt aatcccgtgg caaagccttg
    31381 gaataaagtc gccactgacc aaccaaatat cgcaataaaa taagtcacgc gagaacctac
    31441 gcgatctaaa aaccaaccgc cggggatctg gcatagcgta taaagccagg cgaaggccga
    31501 aaatacatag cccatttccg ctttcgtaat gccgaactct tcctgaatat gggcggaagc
    31561 cacggccagg ttagcgcggt caacataaca aatgaccacc gtaataaaga tcatcaccag
    31621 cgtcagataa cgccgacgcc ccggctttgc tgcattaacg ggaatatcca tagcgagctt
    31681 tctccagatt ttgggcatag cgaagccgct caccatgccc tgtaatttac agagggttat
    31741 atttttgtat tgctgtttta gtgcccgatg aggggcttac gtggcaggaa ttaccactct
    31801 gctacgctgt tatcttcatg acgccagagc ggattacgcc agtccggggc atttttactg
    31861 aactcaataa ctttagcttc gtcgatttcc acacctaagc ccggtttcgt taacggttta
    31921 aagaagccgc caaccatgct gaagtcttct ttgtttttca caaagtcgag taactccgcg
    31981 cctttgttgt aatgaatgcc catactttgt tactgaagta ccgcgttata ggaaacaaag
    32041 tcgatatgca ggcaagccgc cagtgcaatc ggtccgaacg gacagtgcgg cgcaagggtc
    32101 acatcatagg cttctgccat tccagcaatt ttgtagcatt cggtaatacc gcctgcatgg
    32161 gagagatccg gttgcagaat cgaaataccg cctgcctcca gcacgcgttt aaaatcgaaa
    32221 cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc caatttcggg
    32281 tagtattcgg cttgttccgc cagcaccggc tcttcaataa acagcgggcg atacggctcc
    32341 agttctttaa tcagcacttt cgccattggc gcgctgacgc gaccgtggaa atcaagacca
    32401 aactcaatct gattgccaaa agcttcacga atttgtgcca cggtgttgac tgccgcatct
    32461 accgcgcggg agttatcaat tagccccagt tcttcacaac cgttcagttt gaaggtatcg
    32521 aagccgattt cgcgcagggt tttaatgccg tcgataacat ccgccggacg atcgccgccg
    32581 acccaactgt aggctttaat tttgtcgcga accaggccgc ccatcagttg ccagactggc
    32641 gcattcagca ctttgccttt gatatcccat aacgcctggt cgatcccggc gatggcgctc
    32701 atcaggattg gaccgccacg ataaaatccg gcgcgataca tcacttgcca taagtcgttg
    32761 atgcgcgaag gatcctgacc aatcaaatag tcacccagct cgtgaactgc cgcttccacc
    32821 gtacgggcgc ggccttcaat cacgggctcg ccccaaccga ccacgccttc atcggtttca
    32881 attttcagga acatccagcg gggaggtaaa cgatacgtgg taattttggt gattttcatt
    32941 gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg cgttctacgg
    33001 actgcccggc gcgataaaga tcgctgccta agcccgcccc aacacaacct gcgtctatcc
    33061 actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg tccggtggta
    33121 ataccgcttt taacgctttg atgtattgcg gaccaaaagc cgatgacgga aatattttca
    33181 gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg cagccggggc
    33241 aaacggtcat gccgtagccc accgcgcggc ggatcacttc actatggata ttgggcgtaa
    33301 cgatgagctg acagcccatc ctggcgagcg catcgacctg ttcaggtttc agtaccgtac
    33361 ctgcgccaat caacgccttg tcgccatacg catcaacgat ggcgggaatg ctctgctccc
    33421 attgtgggga attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc accgcgccaa
    33481 catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc gggagtttag
    33541 tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc gcccgccacc
    33601 gccgccacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt cagcgatgtt
    33661 ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt cgcgcatact ggcaacctct
    33721 gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggggaag tgttcccagc
    33781 acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg cgggcgcatt aaggccgcgc
    33841 tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc tgcgccaatc
    33901 aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg aaaatcgttg
    33961 atttgctggc tatcggcctg cacccatttg caatgcgtac cgggcataac ataaagagag
    34021 gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat cacattgtgg
    34081 ttatcgtcat gagagacgca taatccggga ataatccaga tattgtcgcc aacagacgtt
    34141 aattgttcgc caatagacga aaaacgggca ggaacagata aatacggagc aactttccag
    34201 ccgacattgc tgccgaccat tcctgccatc accactggcg tattttcttc acgccagtcg
    34261 gtcgtgactt ctgctaacac tgcagccgga gattttccgt tcaggcgcgt gacgcctgct
    34321 tctgattgcc tgctctccag gcagtggtcg ccctgataaa gccaggcgcg cagattggtc
    34381 gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac cttcgtgtcg
    34441 agctggcaat catggtaagc gccgcctgct ctgccgcatc gccgtcctga tgccgtatcg
    34501 catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca tcgcccatcc
    34561 aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt tgctgtaaca
    34621 ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct tcgttaaacg
    34681 cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc tgcgccagat
    34741 cgctggaggt cgcgcgctct gctgcccaac gggcaatcgc cggttccacc agatttcgca
    34801 cttcgctcat ggcactgata agccgtgggt cgtagtcgtt ttccagcacc cattgcagta
    34861 cgtcagtgtc gaggtaattc cactggttac gcggtgccac aaacgccccg cgataacgtt
    34921 tcatttcaat cagccgcttc gccatcagcg aacggaacac ctcacggatg atgttgcgcg
    34981 aggttgcaaa ctcttcacag agctccgcct cagccggaag tggcgagcca ggtacgtatt
    35041 tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg atcggtttta ttgagagtca
    35101 tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat gcataacgcg
    35161 gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc gcgcgcgtaa tgtagtacaa
    35221 acttatatta ttgcgctaca atttagatca aaaaaagaac aacacataaa aaaatgacat
    35281 gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa caggagggaa agaagagtat
    35341 gctttcaacg gcttagctac tcgtttaaag gattaatcat gaagttgaat tttaagggat
    35401 tttttaaggc tgccggttta ttcccactgg cgctgatgct ttcaggctgt atctcgtatg
    35461 ctctggtttc ccataccgca aagggtagtt caggaaagta tcaatcgcag tcagacacca
    35521 tcactgggct atcgcaggca aaagatagta atggaacaaa aggctatgtt tttgtagggg
    35581 aatcgctgga ttaccttatc actgatggtg ccgatgacat cgttaagatg ctcaatgatc
    35641 cagcacttaa ccggcacaat attcaggttg ccgatgacgc aagatttgtt ttaaatgcgg
    35701 ggaaaaagaa atttaccggc acaatatcgc tttactacta ctggaataac gaagaagaaa
    35761 aggcactggc aacgcattat ggttttgcct gtggtgttca acactgtacc aggtcactgg
    35821 aaaacctaaa aggcacaatc catgagaaaa ataaaaacat ggattactca aaggtgatgg
    35881 cgttctacca tccatttaaa gtgcgatttt atgaatacta ttcacccaga ggcattccgg
    35941 atggtgtttc cgcagcatta ctgccagtga ctgttacact ggacatcatt actgcaccgc
    36001 tgcaatttct ggttgtatat gcagtaaacc aataatcagt aagcgggcaa acgcgtttat
    36061 gctgtttgcc cgcccacaga ttaattcagc acatacttct caatagcaaa cgccacgcca
    36121 tcttcaaggt tggattttgt gacaaagttc gccacttctt tcaccgaagg aatagcgtta
    36181 tccatcgcca caccgacgcc tgcatattca atcattgcga tgtcgttttc ctgatcgcca
    36241 atcgccatga tttcttccgg tttaatacct aacacgtcgg ccagtgattt cacacccgta
    36301 cctttgttaa cgcgtttatc gaggatttcg aggaagtacg gcgcactttt cagcacggta
    36361 tatttctctt tcacttcctg cggaatacgc gcgatagcct ggtcgaggat ggcgggttca
    36421 tcaatcatca tcactttcag gaactgggta ttggggtcca ttttctccgc ttcgcagaac
    36481 accagcggaa ttgtggcaac gaaggattca tgcaccgtgt agtagctgat atcgcggtta
    36541 gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc gcgagagagt
    36601 ttttccagga aacgatagtc gtcatagctg agagcggttt gcgccacggt gctaccatca
    36661 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
    36721 tgcagctctt tcaggtagtt gtgcacacct gcatacggac gacccgtcgt tagcacgaca
    36781 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
    36841 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
    36901 caaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
    36961 ccgagggaaa aggggatgca acgcgggttg catgctcccc atttgcttag cggaagagtt
    37021 tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt gctgacactt
    37081 caccttgcgg tgagagcgca tcaataatct tcggcaattg ctctgccagt aaactggaag
    37141 ccgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt gtgccgagcg
    37201 ccgactccag ttgctcgccg ctaactgatt gatttctctg ttgattactc aaccaggttg
    37261 agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga atgccaccct
    37321 gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc agaaaggcac
    37381 cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt gttgacccaa
    37441 aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg cggagatgat
    37501 ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttac ccggcctaca
    37561 aaatcatgca aaatcaatat attgcaggag tctcgtagga ctaataagcg tagcgcatca
    37621 ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca gggcgttctc
    37681 ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga acaactggtc
    37741 agcggcaatc gcatccttaa cggtaacgcg cagcatacga cggctttccg ggtccatggt
    37801 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    37861 gccacgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
    37921 acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccacgca gtttctcacc
    37981 cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca gcggatagtc
    38041 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt
    38101 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    38161 cagcgcgttc acccagcggg taacggtctg ctcatcagaa aggtcagctt ccgtcaacgt
    38221 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    38281 cattttctgc gtcgcgttgt actcagatac cagtttctct aacgcttcgc cagccaatgc
    38341 cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga tctggtactg
    38401 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
    38461 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    38521 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    38581 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacagccaag
    38641 cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc gcgctttctc
    38701 gacgttaagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    38761 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa gcgccggatc
    38821 gcgttcctgg cagtctgcca gtttgcccgg caggccagct aagtcgagcg cacctttacg
    38881 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
    38941 gccaaccacg attttcgcat cagttgggtt ttccagcagg tattccgcca gcagttcgtt
    39001 catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
    39061 ggagaatttc gggtccggca cttttacgga aacgaccgca atcaggcctt cacgcgcatc
    39121 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcatt
    39181 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    39241 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcagcgccac
    39301 ttcaacgcca ataccgtctt tttcggtgga gaagtagaag atatttgggt ggatcggcgt
    39361 tttgttcttg ttcagatatt caacgaacgc tttgatgccg ccttcatagt ggaagtggtc
    39421 ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    39481 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    39541 gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
    39601 cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctgaat
    39661 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    39721 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    39781 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
    39841 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    39901 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    39961 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    40021 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    40081 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    40141 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
    40201 ttaagcggct ggcaagcaac ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    40261 tcaggtagag acaccgccgc ccgctttcac gagtgaggaa ctctccttgc gccagacgta
    40321 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    40381 cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat
    40441 cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc cagccgcgct
    40501 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca tccatatcgg
    40561 ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca
    40621 gttctttatc ccacgggcgt agctgttcgt aacgagtcac ctggcgcagc gccgcattgc
    40681 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccaggt tcgttgtgaa
    40741 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    40801 cttctggcgt tatcaactgc atcggcatca ggtgcgccag ttccgcgact ttatgcccgt
    40861 ccgtaccgtc aatacggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
    40921 tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa tgcctcctgc tcatggcgaa
    40981 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
    41041 ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata
    41101 aagcgagatc cgcggtttca atattgcgga aatcgcggat caacaagcgg gtgagggaca
    41161 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
    41221 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gcgcgttcag
    41281 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    41341 gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcagtaattt tcagctggtt
    41401 ttcgctgaca tacaggcgca caccgcggaa tttctcgtta gagagaatcg ccgcgcgagc
    41461 aaacgcctgc ttgagcagat cgcagcctgc ttccagatgt ttgtccgggt tcttcggcag
    41521 aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaaa taaagtcgcc
    41581 aacgtgggca cgaatgttat tgctgccaat ctgcacacgc agcagattgt cgccgccgtc
    41641 gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat ggcttggcaa
    41701 agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg
    41761 cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa catcctgatg
    41821 cgccatcgaa aactgggttg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
    41881 ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc gccgctggca gggtagacag
    41941 cgaaaaacgg ctacgcccgg agcgcaccag catccgttca ccttccagct gcacggcaat
    42001 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    42061 cgctcctggc tcgtgtggct gaaccagcgc aacgcgtgcc accatttcca tctcgagatc
    42121 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    42181 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
    42241 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa tttgaaaaat
    42301 cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag gcatgaagca
    42361 ccgtcgtgtg gtcacgccca ccaaacgcat cgccaatctc tggcagactg tggttagtca
    42421 gctctttcgc tagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct
    42481 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    42541 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    42601 cgatggtgat cgcccgtccg gtaaagttgg cgttagcaat aacgcggttc agcgcccctt
    42661 ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact tcacccggca
    42721 aacgaatgtc attttcgtcg gcctttttca tcaggatcgc cacacgggtt tccagctctg
    42781 gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc
    42841 cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca
    42901 gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    42961 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    43021 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    43081 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    43141 ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg ttatccgcca
    43201 cctggcaagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    43261 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg acgttatccc
    43321 aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    43381 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    43441 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    43501 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
    43561 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    43621 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    43681 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    43741 aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    43801 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
    43861 ctcacagatt ttccctattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    43921 ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    43981 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
    44041 gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc gtacattttc
    44101 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggtgtgta
    44161 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    44221 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    44281 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    44341 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    44401 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    44461 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    44521 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    44581 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgcctgacgc
    44641 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    44701 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    44761 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    44821 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    44881 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    44941 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    45001 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    45061 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    45121 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    45181 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    45241 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
    45301 ccgttccagc tgttggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    45361 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa
    45421 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    45481 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    45541 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc gtttggtcag
    45601 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    45661 cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa atacaagttc
    45721 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
    45781 ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat
    45841 accgctaatc tgggtaacgg catcgtcgct atcggctata aatctcagcc ggtactggtt
    45901 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    45961 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    46021 atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
    46081 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    46141 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
    46201 cgtctgggcg atgacaaaca gcgtatcagt caggaaatga tggcgctgta caaagctgag
    46261 aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
    46321 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    46381 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
    46441 ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca gaagatcatg
    46501 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtactg
    46561 tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg
    46621 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    46681 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgca
    46741 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    46801 acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
    46861 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    46921 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    46981 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    47041 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt
    47101 tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc gattgccgat
    47161 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    47221 ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat ttacgtcgaa
    47281 gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    47341 cagcttaatg acgtgatggc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt
    47401 ttgttgcgcg aagggatgaa agtagtgatt gccggacgtc ctaatgccgg taaatcgagc
    47461 ctgttaaacg cgctggcggg ccgtgaagcg gcaatcgtaa ccgatatcgc cggaaccacg
    47521 cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgacact
    47581 gccgggctac gtgaggccag tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag
    47641 gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacgac agacgccgtt
    47701 gatccggcag agatctggcc ggaatttatc gcccgtctgc ccgcgaaact gccgatcacc
    47761 gtggtacgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt
    47821 cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat
    47881 ctcaaacaga gcatgggctt tgacaccaat atggaaggcg gcttcctggc gcgccgtcgc
    47941 cacttacagg cgctggaaca ggcagcggag catttgcagc aaggtaaagc gcaactgttg
    48001 ggagcctggg caggtgaact gctggcggaa gagttgcgcc tggcacagca gaacttaagc
    48061 gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc cagcttctgt
    48121 attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg cttcgcttca
    48181 ttgttagcac tcctgttatg cctcaaccct tttttaaaca ttaaaattct tacgtaattt
    48241 ataatcttta aaaaaagcat ttaatactgc tccccgaacg attgtgattc gattcacatt
    48301 taaacaattt cagaatagac aaa
//
LOCUS       DAJTPG010000019        47955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTPG010000019 DAJTPG010000000
VERSION     DAJTPG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO230_001154"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO230_001154"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5690992.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO230_001155"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO230_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5690993.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO230_001156"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO230_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5690994.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO230_001157"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO230_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5690995.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO230_001158"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO230_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5690996.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO230_001159"
     CDS             5377..5835
                     /locus_tag="OO230_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5690997.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO230_001160"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO230_001160"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5690998.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO230_001161"
     tRNA            6887..6971
                     /locus_tag="OO230_001161"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO230_001162"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO230_001162"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO230_001163"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO230_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5690999.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO230_001164"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO230_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5691000.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO230_001165"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO230_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5691001.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO230_001166"
     CDS             complement(10252..13995)
                     /locus_tag="OO230_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5691002.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO230_001167"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO230_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5691003.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO230_001168"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO230_001168"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5691004.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO230_001169"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO230_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5691005.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO230_001170"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO230_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5691006.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO230_001171"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO230_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5691007.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO230_001172"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO230_001172"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5691008.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO230_001173"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO230_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5691009.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO230_001174"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO230_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5691010.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO230_001175"
     CDS             23392..23499
                     /locus_tag="OO230_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5691011.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO230_001176"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO230_001176"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5691012.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO230_001177"
     CDS             complement(24538..25347)
                     /locus_tag="OO230_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5691013.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO230_001178"
     CDS             complement(25351..25743)
                     /locus_tag="OO230_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5691014.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO230_001179"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO230_001179"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5691015.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO230_001180"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO230_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5691016.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO230_001181"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO230_001181"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5691017.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO230_001182"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO230_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5691018.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO230_001183"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO230_001183"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5691019.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO230_001184"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO230_001184"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5691020.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO230_001185"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO230_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5691021.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO230_001186"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO230_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5691022.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO230_001187"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO230_001187"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5691023.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO230_001188"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO230_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5691024.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO230_001189"
     CDS             36790..39657
                     /locus_tag="OO230_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5691025.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQQNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO230_001190"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO230_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5691026.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO230_001191"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO230_001191"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5691027.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO230_001192"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO230_001192"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5691028.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO230_001193"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO230_001193"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5691029.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO230_001194"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO230_001194"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5691030.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYRDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO230_001195"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO230_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12041 a        12344 c        11741 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaaa ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcattac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagcaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct accgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaat
//
LOCUS       DAJTPG010000020        47438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTPG010000020 DAJTPG010000000
VERSION     DAJTPG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            90..572
                     /gene="sseA"
                     /locus_tag="OO230_001196"
                     /pseudo
     CDS             90..572
                     /gene="sseA"
                     /locus_tag="OO230_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1391..2167)
                     /gene="sseB"
                     /locus_tag="OO230_001197"
     CDS             complement(1391..2167)
                     /gene="sseB"
                     /locus_tag="OO230_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5691031.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2309..3592)
                     /gene="pepB"
                     /locus_tag="OO230_001198"
     CDS             complement(2309..3592)
                     /gene="pepB"
                     /locus_tag="OO230_001198"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5691032.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3651..3851)
                     /gene="iscX"
                     /locus_tag="OO230_001199"
     CDS             complement(3651..3851)
                     /gene="iscX"
                     /locus_tag="OO230_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5691033.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3863..4198)
                     /gene="fdx"
                     /locus_tag="OO230_001200"
     CDS             complement(3863..4198)
                     /gene="fdx"
                     /locus_tag="OO230_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5691034.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4200..6050)
                     /gene="hscA"
                     /locus_tag="OO230_001201"
     CDS             complement(4200..6050)
                     /gene="hscA"
                     /locus_tag="OO230_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5691035.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6067..6582)
                     /gene="hscB"
                     /locus_tag="OO230_001202"
     CDS             complement(6067..6582)
                     /gene="hscB"
                     /locus_tag="OO230_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5691036.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6678..7001)
                     /gene="iscA"
                     /locus_tag="OO230_001203"
     CDS             complement(6678..7001)
                     /gene="iscA"
                     /locus_tag="OO230_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5691037.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7018..7404)
                     /gene="iscU"
                     /locus_tag="OO230_001204"
     CDS             complement(7018..7404)
                     /gene="iscU"
                     /locus_tag="OO230_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5691038.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7432..8646)
                     /gene="iscS"
                     /locus_tag="OO230_001205"
     CDS             complement(7432..8646)
                     /gene="iscS"
                     /locus_tag="OO230_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5691039.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8758..9246)
                     /gene="iscR"
                     /locus_tag="OO230_001206"
     CDS             complement(8758..9246)
                     /gene="iscR"
                     /locus_tag="OO230_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5691040.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9516..10256)
                     /gene="trmJ"
                     /locus_tag="OO230_001207"
     CDS             complement(9516..10256)
                     /gene="trmJ"
                     /locus_tag="OO230_001207"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5691041.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10375..11178
                     /gene="suhB"
                     /locus_tag="OO230_001208"
     CDS             10375..11178
                     /gene="suhB"
                     /locus_tag="OO230_001208"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5691042.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11323..12177
                     /locus_tag="OO230_001209"
     CDS             11323..12177
                     /locus_tag="OO230_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5691043.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12368..13648
                     /gene="csiE"
                     /locus_tag="OO230_001210"
     CDS             12368..13648
                     /gene="csiE"
                     /locus_tag="OO230_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5691044.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13640..14779)
                     /locus_tag="OO230_001211"
     CDS             complement(13640..14779)
                     /locus_tag="OO230_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5691045.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14939..15829)
                     /gene="hcaR"
                     /locus_tag="OO230_001212"
     CDS             complement(14939..15829)
                     /gene="hcaR"
                     /locus_tag="OO230_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5691046.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15965..17326
                     /locus_tag="OO230_001213"
     CDS             15965..17326
                     /locus_tag="OO230_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5691047.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17323..17841
                     /locus_tag="OO230_001214"
     CDS             17323..17841
                     /locus_tag="OO230_001214"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5691048.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17841..18161
                     /locus_tag="OO230_001215"
     CDS             17841..18161
                     /locus_tag="OO230_001215"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5691049.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18158..18970
                     /gene="hcaB"
                     /locus_tag="OO230_001216"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18158..18970
                     /gene="hcaB"
                     /locus_tag="OO230_001216"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5691050.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18980..20202
                     /locus_tag="OO230_001217"
                     /pseudo
     CDS             18980..20202
                     /locus_tag="OO230_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20299..20721
                     /locus_tag="OO230_001218"
     CDS             20299..20721
                     /locus_tag="OO230_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5691051.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20769..21641)
                     /locus_tag="OO230_001219"
     CDS             complement(20769..21641)
                     /locus_tag="OO230_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5691052.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21653..22747)
                     /locus_tag="OO230_001220"
     CDS             complement(21653..22747)
                     /locus_tag="OO230_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5691053.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22780..23778)
                     /locus_tag="OO230_001221"
     CDS             complement(22780..23778)
                     /locus_tag="OO230_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5691054.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23803..25314)
                     /locus_tag="OO230_001222"
     CDS             complement(23803..25314)
                     /locus_tag="OO230_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5691055.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25330..26324)
                     /locus_tag="OO230_001223"
                     /pseudo
     CDS             complement(25330..26324)
                     /locus_tag="OO230_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26421..29522)
                     /locus_tag="OO230_001224"
     CDS             complement(26421..29522)
                     /locus_tag="OO230_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5691056.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29529..29702)
                     /locus_tag="OO230_001225"
     CDS             complement(29529..29702)
                     /locus_tag="OO230_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691057.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29820..31013
                     /locus_tag="OO230_001226"
     CDS             29820..31013
                     /locus_tag="OO230_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5691058.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31211..32464)
                     /gene="glyA"
                     /locus_tag="OO230_001227"
     CDS             complement(31211..32464)
                     /gene="glyA"
                     /locus_tag="OO230_001227"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5691059.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32791..33981
                     /gene="hmpA"
                     /locus_tag="OO230_001228"
     CDS             32791..33981
                     /gene="hmpA"
                     /locus_tag="OO230_001228"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5691060.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34026..34364)
                     /gene="glnB"
                     /locus_tag="OO230_001229"
     CDS             complement(34026..34364)
                     /gene="glnB"
                     /locus_tag="OO230_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5691061.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34425..35759)
                     /gene="glrR"
                     /locus_tag="OO230_001230"
     CDS             complement(34425..35759)
                     /gene="glrR"
                     /locus_tag="OO230_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5691062.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35749..36462)
                     /gene="qseG"
                     /locus_tag="OO230_001231"
     CDS             complement(35749..36462)
                     /gene="qseG"
                     /locus_tag="OO230_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5691063.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36627..38054)
                     /gene="qseE"
                     /locus_tag="OO230_001232"
     CDS             complement(36627..38054)
                     /gene="qseE"
                     /locus_tag="OO230_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5691064.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38612..42499)
                     /gene="purL"
                     /locus_tag="OO230_001233"
                     /gene_synonym="purI"
     CDS             complement(38612..42499)
                     /gene="purL"
                     /locus_tag="OO230_001233"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5691065.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42758..44314
                     /gene="mltF"
                     /locus_tag="OO230_001234"
     CDS             42758..44314
                     /gene="mltF"
                     /locus_tag="OO230_001234"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5691066.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44311..44847)
                     /gene="tadA"
                     /locus_tag="OO230_001235"
     CDS             complement(44311..44847)
                     /gene="tadA"
                     /locus_tag="OO230_001235"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5691067.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44872..45507)
                     /gene="yfhb"
                     /locus_tag="OO230_001236"
     CDS             complement(44872..45507)
                     /gene="yfhb"
                     /locus_tag="OO230_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5691068.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45716..46564
                     /locus_tag="OO230_001237"
     CDS             45716..46564
                     /locus_tag="OO230_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5691069.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46903..47438)
                     /locus_tag="OO230_001238"
                     /pseudo
     CDS             complement(46903..47438)
                     /locus_tag="OO230_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11312 a        12993 c        11936 g        11197 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg gacttgcagg ctggcagcgc gatgacctgc
      121 tattagaaga aggtgcagta gagctgccgg aaggagagtt taacgccgcg tttaatcctg
      181 aagcagtggt gaaagtaacc gatgtgttat tggcaagcca tgaaaatacg gcgcaaatta
      241 ttgatgcccg cccggctgca cgttttaacg cagaagttga tgaacctcgc ccaggtttac
      301 gtcgcggaca tattcccggt gcattgaacg ttccgtggac ggaactggtg cgcgaaggcg
      361 aactaaaaac gaccgatgaa ctggatgcga tattttttgg tcgcggcgtc agctatgaca
      421 aaccgattat cgtcagctgc ggctctggtg taacggcagc cgtggttttg ttagcactcg
      481 cgacgctgga tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa tggggcgcgc
      541 gggcagattt accggttgag ccagtgaaat aagtatatta caggcaataa aaaaccgccg
      601 aatttggcgt ttttttattg ctggtctggt tcgcggcctt tccagcaggt tgacttgtgt
      661 tacatgagca acgcaggtgc ttcacagcaa aacaatactc accagtaact ctctttttgt
      721 caagcaaaag agagtaatta ttgtttattt agcgtattat cgacaccggc cctttccgcc
      781 gtgttcggta ataaaataac ctggcttatt agtccgaatt cagacaaata taaacaaatc
      841 ctgctcaaaa ttaaaaattc taaccggtaa aagatattac ttaaacatgt aaatttactt
      901 tcctttaaaa aacaaaaaac cgccaaaatc aggcggttat ttgttgctgg tccggttcgc
      961 ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac
     1021 tcgccagtaa ctctcttttt gtcaagcaaa agagagttat tattgttctg ttagtgtatt
     1081 atccactgcg gccctttccg ccgtctcgca agcgggcgct ggcttaagga aaggatgttc
     1141 cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg cggcaatatc gccagtggtg
     1201 ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt ctgtatcgta
     1261 cttattgttt ctggtgcgct gttttcccaa gtaaataata accggagtct ctccggcgac
     1321 aatttactgg tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttagg
     1381 gcggcatatt ttagattatc cggttttgtt taaagtcacg caaaaaacca ccccagcgac
     1441 gttcatagaa tggcgcaata tgttcggtaa taaagtggct aattcctttt tccccttttt
     1501 tcacctgaca aatatcgatt ggttcatcgc caggtaatgt atcggtcgct acacttcccg
     1561 tcgcctgaat aatttcttcg atatcaccat cggcttcaat gccaataagt aaattaggct
     1621 gtgcctcttc gttctcttta attgaacaaa taaaagcacg cttcaccggc ttaatggttt
     1681 taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg ctctgcgact tccgataata
     1741 tcagcgattc accgccttcc aggatttcct ggctgctcag cggatttccc tcttcaccaa
     1801 tcaacaaact gatttcacgc ggcataaatt ctttaccggt cggcagtttg gcattaagga
     1861 agagcgtttc gccaagggtc atctcaaaca atgtgcgaac gggcattacg acaaatgcct
     1921 gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt gaaaaaagga atgacgctgg
     1981 tgccgtcttc tttttcccag tgctgtaaat caagcgcact atcttcaacc acagcctcgc
     2041 cctgcgccgc cgtaccaggc acccagacgg tggattccag tagagtacgg aaaaaggccg
     2101 ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg ttttttgttt
     2161 cggacataag aaccacaatt cattcaacgt tcggggcgca aatgcctgat gcgctacgct
     2221 tatcaggcct acaagggatt cgcaatttgt tgaatttgca gaatttgtag gccggataag
     2281 gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagct atcgtgcgca
     2341 caccaagtcc cgtagcgccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg
     2401 agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac aggaagcccg
     2461 ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt attgttcagt tcggcaaagt
     2521 tagacggcag ctggctgcgg tggaattccg ccagcggcag acgccagaat ggttcgttct
     2581 cttgtgaggc actcgccagt aaacgaccgg caagcgcatc gtcaaaactg aacagcgcgt
     2641 gataatcatt acccagcgca gttttcgccg ccccggtgag ggtcgccgca tcaatgatca
     2701 tttccggttt ctgcgcactg gcatcaatca gaccatcggc aagcaccaga cgcccttcgg
     2761 catcagtgtt catcacttca acttttttac cgttgcgata ggtgatgata tcgcccagct
     2821 tgaacgcatt gccgctaatc aggttatccg cacagcagag gaacagcttc acgcgcttgt
     2881 tcagtccgcg cataatggca aatgccagcg ccccggtaac cgttgccgcg ccgcccatgt
     2941 ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg gagtcaaaag
     3001 tgataccttt acctaccagg cacgcgtaca ccggtgcttc tttatcgcca gttgggttgt
     3061 aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg cccgacagtg tgcagcccca
     3121 tataaccttg ctcacgcaga tcttcgcctt tggtgatccg ataagtcaca cgatcgcccg
     3181 cgacgttgct gatcagatca acagcacgct gtgccagttg cgatggtccc aattcttctg
     3241 ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct
     3301 ggcgctgggc atcgtccaga tccggccact ccactttacg cgtgcctttc gggcctttat
     3361 aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat
     3421 gcttgatgcc cagaccgtca atcttgcggg ccgcacgctg gatcagcccc agatcgtctg
     3481 ccccgttcaa atgcagggta atgccgtcat tattaatgct gtaagttgct ttttctcccc
     3541 agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat cgcttctgtc attttagtta
     3601 tccttattcg tttaacagca aaacgggcta cccccaggca gcccgttcat tcactcggct
     3661 tcgtccagcc agactaacaa aatcgcttcg aggattttct cgttggatgc ctgcgggtcg
     3721 tcgtcgaaat cttccagatc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc
     3781 ggatcaagat cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccac
     3841 ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga
     3901 tttcgaccac taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct
     3961 ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagtgagt
     4021 caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt
     4081 gttcaatctc aataccgtta cgcagcgctg cgtcgagaat ggtttcaccg ctattagctt
     4141 ccagaacagc gccatcaggg cagaggtcct gatgaggcaa aataacaatc tttggcatat
     4201 taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga
     4261 gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca
     4321 tcatcgccct gcgccacttc actcaggtga gcggcagcat tgtcaatgac ctgacgctct
     4381 gcggcgctta acagcgcggc atcagcagcc agcgcgccgt gcagactttc cagcacacgc
     4441 gccgcttcta ctttttgttc tgccagcata cgggctttta cgtcctgctc ggcatagctc
     4501 attgagtctt tgatcatcga agcgatttcg ctatcggtca gaccgtaaga cggtttgacc
     4561 tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg
     4621 gcatcgacct ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc cggaatacca
     4681 cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca gttcgcgctc accctgcatt
     4741 acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg agcgcgggcc
     4801 accggaatag tggtattacg cggaatcact ttctccacca ggccgcccat cgtttcgagg
     4861 cccagcgaca gtgggatcac atcaagcagc agcatttcgc tgtctggctt gttacccacc
     4921 agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatggaagtc
     4981 agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca ccagcggcac gcgagtagaa
     5041 ccgcccacca tcaccacttc cagcacttcg tcagcttcta cacccgcgtc tttcagcgcg
     5101 cgacgacaag ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca
     5161 cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagtccgc atcgctcagc
     5221 gcgattttgg ctgcaatggt ggcatccagc agttcacgct gaacgcggtt atcgctacga
     5281 tcaggaatgt ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg
     5341 ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca cgccgcgact taagcgcaga
     5401 atggaaatat caaacgtccc gccaccgagg tcataaacag cgatcacgcc ttcctgaccg
     5461 gaatccagcc cgtaggcgat agccgcagca gtcggttcgt taagtaagcg cagaacatgc
     5521 aaccccgcca gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgcc
     5581 ggaacggtga taactacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc
     5641 agtgctttga ggatgtccgc agaaacgcgc accgggttca gtagccccgc cgccgtttca
     5701 atcatcggca ggccgttttc gctggcctgg aattgataag gcagatgcgg atagcgttgc
     5761 tggatatcag ccagcgagcg ccccatcagg cgtttaacag aactaatggt gttggcggtg
     5821 tcaagcgctg cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga
     5881 acaacagatg gcagcaggtg acggccttca tggtcggcta acgtttcggc ctgaccgctg
     5941 cgcactgtcg ccaccagcga gttggttgtg cccaggtcaa taccagccgc cagacgacgc
     6001 tgatgcggcg cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc
     6061 cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc
     6121 gagaaaacgc agcttacgca ccgtatccgc cgccgcgtcc cacgcctcgt tgtctaactg
     6181 ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc
     6241 cagccgcgct tcatctttcg cctgttcgat ctcgtccagc tcttcgcgca gctccaactg
     6301 ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg
     6361 taaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt
     6421 aatggttgca gattgctgta cggcggcgag ttgttccgcc aggcttccgc tggcgaattt
     6481 atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagtgcct gggtatcgag
     6541 ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg
     6601 atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg
     6661 ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttaacg
     6721 ttcgggttgg tgaatttaaa cccttcattc aggccttctt ttacgaagtc cagctgcgta
     6781 ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac
     6841 acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacat
     6901 ccggaggttc tcacgcccag acgcaggcca aaccctttgc cgcggttagc cagaaaggta
     6961 tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta
     7021 ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag
     7081 aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt
     7141 gatcgcctgc gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga
     7201 agcgatagcg gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc
     7261 gttgacttta atctgcaact tcattacgtc gccacaggcc ggtgcaccca ccatgccgct
     7321 gccgacgttc tcgtcgttgt tatcaaagga acccacgtta cgcggattct cgtaatggtc
     7381 gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg
     7441 agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag
     7501 gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc
     7561 agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc
     7621 cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga
     7681 aactgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac
     7741 gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc
     7801 tttgatgccg ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat
     7861 ctcttctttt gcgatgcgat aggcttcgcc cattccgacg atctggtgaa caggcagagt
     7921 gccggaacgc ataccgcgct cgtgaccgcc gccgtgcatt tgcgcttcga tgcgtacacg
     7981 cggtttacga cgcacataca gcgcaccgat acctttcggg ccatagattt tgtgaccgga
     8041 gaaagacatc aggtcaactt tcaactggct caggtcgatt ggcagtttgc ccacgctctg
     8101 ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat
     8161 atcctgcacc acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcgtc
     8221 acgcatcgcc gcttcgagtt ctttcaggtc gataataccg ttacgctgcg gtgccaggta
     8281 ggtgacttcg aaaccttcgc gctccagctg acggcaagta tccagtaccg ctttgtgttc
     8341 ggttttgctg gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat
     8401 cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc
     8461 gaccagatcg gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa
     8521 acggtgagaa cgagaggccg ggttaccaaa ggttccgtcc atcgtcatga actgcatcat
     8581 tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa
     8641 tttcattgct ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga
     8701 tgtacgaccg tgtttacgga gtatttagca ctccggcctg attctgaatt ctttttatta
     8761 agcgcgtaac ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg
     8821 ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt
     8881 gttgagaaaa ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt
     8941 atcgccgccc tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac
     9001 ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc
     9061 gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc
     9121 cagataagaa agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc
     9181 ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag
     9241 tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg
     9301 gggtattgca tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt
     9361 gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct
     9421 gttgtcggat gcggtgtgaa caccttatcc gacctacgat tctgtacatt gtaggcctga
     9481 taagacgcgt taagcgtcgc atcaggcaac cactgttatt cggccttgtt acctttattc
     9541 tgctgctcaa tagaagccag aatcccgcgc aggatattca attcctggct ttccgggcgc
     9601 gcacgggtaa acagacggcg caatttattc atcacctgcc ccggatggtt ttcacggata
     9661 aaaccggttg ccagcagcgt ttgttccaga tgaccataaa aacgctccag atcatcgacc
     9721 agcggatacg gcgtctcttc atgctcgacc tgctcgccat tttcctgagt tgccagccag
     9781 gccatacgca cttcataggc aataacctga accgccattg ccaggttcag cgaactgtat
     9841 tccgggtttg ccgcaatcgc gacatgataa tggcatttct gcaactcttc attggtcaag
     9901 ccgacgcgct cacgaccaaa caccagcgcc accggggtat ttgccgcttc agcgacgctt
     9961 ttcagaccgc attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga gcgtgcgctg
    10021 gtgcccacca ccaggctaca accggctaac gcttcgtcga gcgtatcgac gatgtgagca
    10081 ttaccaatca catcgctggc ccctgcagcc agggcaatcg cctgggagtc gggtttcacc
    10141 agtggattaa ccagccacag attggttaat cccattgttt tcatggcacg ggcaacagaa
    10201 cccatattgc cggtgtgtga cgtctccacc agcacaattc gaatattttg cagcattgtc
    10261 tttcttcatc taaagattat tcacgcatct tatcataaaa cgaagacaga tgccgatctc
    10321 gctgctatac tctgcgccgt tttcccgttc tttaacatcc agtgagagag accgatgcat
    10381 ccgatgctga acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat tgccaaaaac
    10441 tatgaaaccc cggacgctgt agaagcgagc cagaaaggca gtaacgattt cgtgaccaac
    10501 gtagataaag ctgccgaagc ggtgattatc gacacgattc gtaaatctta cccacagcac
    10561 accatcatca ccgaagaaag cggtgaactt gaaggtactg atcaggatgt tcaatgggtt
    10621 atcgatccac tggatggtac taccaacttt atcaaacgtc tgccgcactt cgcggtatct
    10681 atcgccgttc gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc tatgcgtaac
    10741 gaactgttca ccgccactcg cggtcaaggc gcacagctga acggctaccg tctgcgcggt
    10801 agcaccgctc gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt caaagcaaaa
    10861 cagtacgcca ctacctacat caacatcgtc ggcaagctgt tcaacgaatg tgcagacttc
    10921 cgtcgtaccg gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg tgttgacggt
    10981 ttctttgaaa tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct ggttcgtgaa
    11041 gcgggcggca tcgtcagcga cttcaccggt ggtcataact atatgctgac cggtaacatc
    11101 gttgctggta acccgcgtgt tgtgaaagcc atactggcga acatgcgtga cgagttaagc
    11161 gacgctctga agcattaatg actcaggcgg gtgatatcac tcacccgccc tcgcctttca
    11221 ggcgctattc cgaaatactt cctcaccgct ttactttctt tcgtcactct cccaccatct
    11281 ttccccgatt aatggatgat aaagaagtcg caaggaaagg ttatggcact gccagtgaac
    11341 aaacgcgttc ccaaaattct gtttattctc tttgttgttg ccttctgcgt ttatttagtg
    11401 ccgcgcgttg ccatcaactt cttctattat cctgacgata aaatttacgg tcccgacccc
    11461 tggtcggcgg aatccgtcga atttacggct aaggacggta ctcgtctgca aggctggttt
    11521 attccttctt cgacgggccc tgctgacaac gccatcgcaa ccatcattca tgctcacggc
    11581 aatgccggaa atatgtccgc ccactggccg ctggtcagtt ggttacctga gcgtaatttc
    11641 aacgttttta tgtttgatta tcgcgggttt ggtaaatcaa aaggcactcc gtcccaggcc
    11701 ggattgctgg acgatacgca aagcgccatc aatgtggtgc gccatcgcag tgatgtaaac
    11761 ccacaacagc tggtgctgtt cgggcagagt attggcgggg cgaatattct tgctgttatt
    11821 ggtcagggtg atcgtgaagg catacgtgcg gtgatcctcg actccacatt tgcctcttat
    11881 gcaaccatcg ccaaccagat gatccccggc agtggctact tacttgatga gagttacagc
    11941 ggcgaaaatt acatcgccag cgtcagcccg atcccgcttt tactcattca cggtaaagcc
    12001 gatcacgtta tcccatggca gcacagcgaa aagttgtata gcctggcaaa agagccaaaa
    12061 cggttgatcc taatcccgga tggcgaacac attgatgctt tttccgatcg tcacggcaat
    12121 gtttatcgcg aacagatggt ggactttatc cttagcgcgt tgaatccgca gaactaaccc
    12181 atgatcgcta gcacgataat cattcacaaa accaccttaa gacatgctaa tccactggtc
    12241 agaacagttt aaaatgagaa aaattctgtg acgcttgcca acatttctga tgattagcat
    12301 tcccttcgcc atttccctga gcaaacttta gctattctta tcaattatgc ttatgggaga
    12361 tctacagatg atgcctacgc ttgctccacc atctgtcctt tcggctcccc agcgccgctg
    12421 ccagatcttg ctgacgctct ttcagccggg gttgaccgcc accatggcaa ccttcagcga
    12481 gcttaatggt gtggatgatg atattgccag tcttgatatc agcgaaacag gacgggagat
    12541 cctgcgctat catcaactca cactgacgac tggttatgac ggtagctacc gggttgaagg
    12601 tacagtgctt aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt tccgtctgtg
    12661 tccgtcattt attaccagcc aattcacccc cgccctgaag agtgaactga agcggcgcgg
    12721 aattgcgcgt aacttttacg acgataccaa tctacaagcg ttagtgaatc tctgctcccg
    12781 acggctgcaa aaacgctttg aatcgcgcga tattcatttc ctgtgtctgt atctgcaata
    12841 ttgtttgctg cagcaccacg ctggaattac gcctcagttt aatccgctcc aacgtcgctg
    12901 ggcggaatcc tgccttgaat ttcaggtagc gcaggaaatt ggacgccact ggcagcgtcg
    12961 ggcgctccag catgtaccgc ctgatgagcc actgtttatg gcattacttt tttccatgtt
    13021 gcgggttccc gatccactgc gggatgcgca tcagcgggac agacaattgc gtcagtctat
    13081 caaacgtctg gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg atgaacaggg
    13141 gttatgtgat cagctttata tccacctcgc ccaggcgtta aatcgcagtt tgtttgccat
    13201 cggtattgat aataccctgc cggaagagtt cgccagactg tatccacgcc tggtacgcac
    13261 cacccgcgcg gcgctggccg gatttgaaag tgaatacggc gtccatcttt ctgatgagga
    13321 aagtggtctg gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg atctgcatga
    13381 aaaacagatt attctactga ccgggaatga tagcgagcga gaagcgcaga ttgagcagca
    13441 gttacgcgaa ctaacgttac tgccgctcaa cattaagcat atgtcggtaa aggcattttt
    13501 gcagacaggc gctccgcgtg gcgcggcact gattattgcg ccttatacca tgccgttacc
    13561 gctcttttca ccaccgctga tctatacgga cctgacgctg acaacacatc aacaggagca
    13621 gatccgcaaa atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa aacattgccg
    13681 gaagcgccac cagcgccatt acccagaaca cgccgtggcc cagatattga tacaggaaac
    13741 cggcgaaaac ggtcatgata gcgatactgc cgcccatcgc gacggcagag taaaccgcct
    13801 gtaaacggat gacttcgcta acctggcgag cagcaatata acgcatggcg gccaggtggc
    13861 agaccgtgaa ggtgccgcaa tgcagaattt gcaccactat caaccacggc aacgccgtag
    13921 ttgctcccat aatgccccag cgcactacgc cgcaaatcgc cgagatcaac agcatatcgc
    13981 gagcactaca acggcggaaa agtttattac tcagcgcaaa gataatgact tccgccacca
    14041 cgcccagcga ccacaaatac cccaccgccg aggccgagta gccagctgcc tgccagtaaa
    14101 tggcgctaaa accgtaatag gccgcatgtg ccccctgcaa taaacaaacg caggccagaa
    14161 agcgccagtt ctggcgaacc agcgccaacc acgcagacca accggtgctc tcctgctggc
    14221 ggcttgcccc ttgtggctga atcgtcggac ggatgagaaa gccgagcagc atggatgcca
    14281 cgcccaacgt caacagcacg aggatcaccc gataatcaaa catagtgacc agtttgcccg
    14341 tcagcgccga gccaatgaca aacgccaccg agccccacag tcgcactttg ccgtaatcaa
    14401 gcgggaactg cttttgccac gtattcgcca gtgcatcggt caacggtacc agcggtgaga
    14461 aaaagaggtt aaagccaatc atcaccagca tcagccacgc tacgtgcgcc cccgcccaga
    14521 aggcgacagc aaagagaagt ggcagcagtg ccagcacgcg caaggcggaa atcaggcggg
    14581 aaggatcgct gacgcggggc gcgatgagca aactcccgag aaaacgggca accagacctg
    14641 cccccaataa caggccgatg gtttctggcg ttaaaccaat ccctttaagc cagacgctcc
    14701 agaaaggtaa aaaaatgccg taactaaaaa agtatgtgaa atagccgagc gccaaccagc
    14761 gcgtggattg caaaaccatg agtccctccc gtcaggaggc gttagtctgg ggttaatcga
    14821 atgttggtgc aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt ctgtaacgcc
    14881 agttcacaag cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata atgcgtaatt
    14941 atgccgttac gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt aatgccagct
    15001 tcatctcccc tttcttccac gccatcagta aagcaatgga aggcacattt ccggcaatgg
    15061 gacgaaaaac aacctgcccg gtattaaaat tattcatata accgggtatc aaagtgacgc
    15121 ccagccccat gcccaccaga ttcatggtca ccagaatatt cgttgccacc tggacgatat
    15181 ttggctggct gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc gaaccggaat
    15241 acgccggatc ggtactgacg aaattcacgc catccagttg cgcggcggtg atctcttttt
    15301 catgtgctaa agggtgatca accggtaaca ccaccactaa tggttcgtca aaaagctcca
    15361 gataatcaat ctccgggcta taaacaggat ggcgcattaa gccgacgtcg agttcaccac
    15421 ggcgaatttt ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc aaggtgtctg
    15481 gctgtctgag acgaaacatc ggtaatactt ttggcaataa attcacttcc gccgatggca
    15541 caaagccaat ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt aatttggcat
    15601 tttccgcttg ttcgaggatt gccagcgcgt cctggagaaa acattctccc gccgccgtca
    15661 acgcgacttt gcgcttatcc ctcaccagta acggaacacc gacacagttt tcaagatcgc
    15721 ggatctggct gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca cgggtaaagt
    15781 tcagtgcctg cgccactgcg acgaaatagc gtaaatgccg tagttccatc accttcccct
    15841 tgttatcgaa aaaacgtctc agctggtaga aattaactat ttcacatatt agcaaccaac
    15901 cagcaacatc cttatggcac aaaaatagaa ggtcaataca tcttatcttt caggattaaa
    15961 aaatatgacc acaccctcag atttgaacat ttaccaactg attgacaccc aaaacggtcg
    16021 ggtcactccg cgtatttata ccgacccgga catttaccaa ctggagcttg agcgtatttt
    16081 cggtcgttgc tggctatttc tcgcccacga aagccagatc ccaaaacccg gtgatttctt
    16141 taacacctac atgggagaag atgcagttgt cgtggtgcgc cagaaagacg gcagcatcaa
    16201 ggcgtttctc aaccaatgcc gccaccgggc catgcgtgtg agttatgcag attgtggcaa
    16261 cagtcgcgcc tttacctgcc cgtatcacgg ctggtcttat ggcattaacg gcgagttgat
    16321 cgatgtaccg ctggaacctc gcgcctaccc acaagggttg tgtaaatccc actgggggct
    16381 aaacgaagtt ccttgtgtgg agagttataa aggactgatt tttggcaact gggataccag
    16441 cgcaccgggc ctgcatgatt acctgggtga cattgcctgg tatctggatg gcatgctgga
    16501 tcgtcgcgaa ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga tcaactgtaa
    16561 ctggaaattc ccggcagagc agttcgccag tgaccagtat catgctctgt tcagccatgc
    16621 ttctgccgtt caggtattag gggcgaaaga tgatggcagc gataagcgcc tcggtgatgg
    16681 acaaaccgcc cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg gtcaggacgg
    16741 tcacggtagc ggtttcttct ttactgaaaa accggatgct aatgtctggg tcgatggcgc
    16801 agtttcaagc tattaccgcg aaacctatgc cgaagcagaa caacgtttag gtgaagttcg
    16861 cgccctgcgc ctggcgggtc ataacaatat attccccacg ctttcatggc taaacggcac
    16921 tgccacactc cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt gggcgttctg
    16981 tattactgac aaagccgcct ccgatgaagt taaagccgct tttgaaaaca gcgccactcg
    17041 tgcttttggt cctgctggtt ttctcgagca ggatgactcg gagaactggt gtgaaatcca
    17101 gaaattgctt aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa tggggcttgg
    17161 tcaggaaaag cgccgcgacg acggcattcc tggcattact aactatatct tttcagaaac
    17221 tgccgctcgt ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa gctggcagga
    17281 agtgctcgat aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc gcaagtttca
    17341 ctggagttac atcaccgcat tagccagttt ctctttcacg aagccagtct gctggatgac
    17401 tggaaatttc gtgactggtt ggcacagctc gacgaagaga ttcgttacac catgcgcacc
    17461 acggttaacg cgcaaacacg cgaccgtcgc aaaggcgtcc agccgccgac gacctggatt
    17521 ttcaatgaca ccaaagacca gctggagcgg cgaatcgccc gtctggaaac gggcatggcc
    17581 tgggcagaag agccgccgtc acgcactcgt cacttaatca gcaactgcca ggtaagcgaa
    17641 accgacatcc caaacgtatt tgccgtgagg gtgaattatc tactttatcg ggcacaaaaa
    17701 gagcgcgatg aaacattcta tgttggaacg cgtttcgaca aagttcgccg tctggaagat
    17761 gacaactggc gcttgctgga acgggatatc gtcctggatc aagcggtgat cacttcccat
    17821 aacctgagtg tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg gatgtgccgg
    17881 agggtgaggc tctccggata gatacctcgc ccgtcatcgc cttgttcaac gtgggcggcg
    17941 agttttatgc cattaacgat cgttgcagcc atggtaatgc gtcgatgtca gaagggtatc
    18001 tggaagatga cgccacggtg gagtgcccgc tacacgccgc cagtttttgc ctgaaaacgg
    18061 ggaaagcgtt atgcctgccc gccaccgatc cgctcaccac ttatccagta cacgttgaag
    18121 gtggtgacat tttcatcgac ttaccggagg cgcagccatg agcgatctgc ataacgagtc
    18181 catttttatt accggcggcg gatcgggatt agggctggcg ctggtcgagc gatttatcga
    18241 agaaggcgcg caagttgcca cgctggaact gtcggcggca aaagtcgcca gtctgcgtca
    18301 gcgatttggc gaacatattc tggcggtgga aggtaacgtg acctgttatg ccgattatca
    18361 acgcgcggtc gatcagatcc tgactcgttc cggcaagctg gattgtttta tcggcaatgc
    18421 aggcatctgg gatcacaatg cctcactggt taatactccc gcagagacgc tcgaaaccgg
    18481 cttccacgag ctgtttaacg tcaacgtact cggttacctg ctgggcgcaa aagcctgcgc
    18541 tccggcgtta atcgccagtg aaggcagcat gattttcaca ctgtcaaatg ccgcctggta
    18601 tcctggcggc ggtggcccgc tgtacaccac cagtaaacat gccgcaaccg gacttattcg
    18661 ccaactggct tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc cgtgtggtat
    18721 ggccagcgac ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga taatgcagtc
    18781 tctgacgccg gagaaaattg ccgccatttt accgctgcaa tttttcccgc aaccggcgga
    18841 ttttacgggg ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat taagcggtgt
    18901 gatgatcaac gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag cggctgggct
    18961 ggatctttaa ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg gcgggcaagc
    19021 ggcggcaatg gctgcggcct cgctacgcca gcaagggttc accggtgagc tgcatctgtt
    19081 ttccgatgag cgacatcttc cttatgaacg acctccactc tcgaaatcca tgttgctgga
    19141 agattccccg cagttacagc aggtgttacc cgctaactgg tggcaggaaa acaatgttca
    19201 tctgcattcc ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt tagtgttaac
    19261 caacggcgaa agctggcact gggatcagct ttttatagca accggcgcgg cagctcgacc
    19321 gctgccgttg cttgatgcac tgggagaacg ctgctttacc ctacaccatg ccggtgatgc
    19381 cgccagactg cgagaagttc tgcagcccga acggtcagtc gtgattgtcg gtgccggaac
    19441 tattggtctg gaactggctg ccagcgccac gcagcgcagc gcagcgcagc gcagcgcaga
    19501 tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc accaccgccc
    19561 gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct gctcaataat
    19621 gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag tggggaaacg
    19681 cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca actggctcgc
    19741 gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg cacctgcgat
    19801 cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg tgcactacac
    19861 cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc cgcaatgtta
    19921 gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta cagtgataac
    19981 ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa cccggaaact
    20041 cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt cacgctgaat
    20101 cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac gtttgatgcg
    20161 aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata attagcgaat
    20221 atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa atcacattat
    20281 cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc tgcccgcccc
    20341 gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt tggttttccc
    20401 aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc gccaatgccg
    20461 atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt aatcgtgctt
    20521 ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg tacggcggtg
    20581 attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat gattaatttc
    20641 tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg accgggggca
    20701 atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct tatccggcat
    20761 tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga ttcccctggt
    20821 gctagcgcaa tgagatcacc cccttccggg cgatgatgct catccggcgc atggctcatc
    20881 ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc ggaaacaaag
    20941 atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc ttcctgcggc
    21001 tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca ctgacgcggc
    21061 aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa ctcacccgcc
    21121 agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg cgtttgtggt
    21181 gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc cccttgattg
    21241 gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg acttacccga
    21301 tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc gctgtgcgag
    21361 acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc ccactcaaca
    21421 ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct cacccggttt
    21481 gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt tttaccagga
    21541 cgtaagagcg gcgtcgtatc gcgccagaag ccttcgatta cgccgccctg gtcagaaacg
    21601 tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact ccttaatccg
    21661 ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag gcatcacctg
    21721 cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc cagtcagtca
    21781 aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg ataatccgcc
    21841 gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt tcaccaatgt
    21901 aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct gcggcattac
    21961 cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt atctgcggca
    22021 gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt gccatcgcca
    22081 gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct ttcgccagca
    22141 tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg ctgccggaaa
    22201 cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata aacgcaccat
    22261 cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc agcagatatt
    22321 cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca gtacaagaga
    22381 taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga tacaccagca
    22441 cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc tgcccgcacg
    22501 gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc gccgtggcac
    22561 ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat ttcattttga
    22621 tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg acggttgaat
    22681 ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca atacgattaa
    22741 ttttcatccc tgcagcaccc gcgccagggg cgttaaggtt tagcgtttcg ctttactgct
    22801 tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc cgcgtaccac
    22861 ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta gcgtaatcac
    22921 cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg ccgtaccgcc
    22981 gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg cgttaccgga
    23041 accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg aaagggtaaa
    23101 gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg tggcattacc
    23161 gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca caaacagcgc
    23221 aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc cgcccagcca
    23281 gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa acagccccat
    23341 tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt taaacactcc
    23401 gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca gcagacaagc
    23461 aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga caaaagccac
    23521 catcggccca acgctgacat caatttcacc ggagataata atcagcgtca tcgcccaggc
    23581 ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca atgagataaa
    23641 gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca gcaaaccgat
    23701 ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct gcggcaatgg
    23761 taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgca cagacagaat
    23821 ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga acgtgccgtg
    23881 ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac tggagataaa
    23941 caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct gctgtttggc
    24001 ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg cggcatagac
    24061 ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga tgggtgtttc
    24121 gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc ggcggatggt
    24181 ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca ctgtattttc
    24241 gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg tatagccaat
    24301 gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt tgataacaat
    24361 ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac tgcgccctgc
    24421 ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc tgatatcctc
    24481 cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca caatttcctg
    24541 aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca caatatgatg
    24601 cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca taacggtggc
    24661 ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca ccgccacgcc
    24721 cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac tggcaagcga
    24781 gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc gtgcaatttc
    24841 caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac taacgtcaac
    24901 gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat caatcatgcc
    24961 gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc cttccaccag
    25021 acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag tcaacgtagc
    25081 ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg ggcgttcgct
    25141 gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta acagcgcacg
    25201 aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc cggggtaacg
    25261 cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga acatagggac
    25321 ctctgcgaat cagcgattag ggcagagcag accatcaacg tgcgttgcca gccactgttt
    25381 accatcttcc gttttggtat agagatcgat tggcacctga atcacttttt caccgtcggc
    25441 ttgtttattg ataaccttca atgtttgcgc gaaaacagca ttgcccattt tcttaccgga
    25501 aatatccact accgctttca gcacctgatt gttttccagc tcctgagcaa tttcggttgt
    25561 catatccgaa ccgaaaacag caatttttcc ggcctgattt tgattacgta ccgcttttac
    25621 cgcgccgagt gtcgcaccgc ccgactcccc cataatggcg ttgagatccg gcgtggagat
    25681 aatcagtttt tcaccaacgg aaatcgcttt atctaaaaca gtcccttcct ggttagcgac
    25741 aatttgcgcg ccgggaacgc gggattttaa tacttcttca aatcctttac gtcgctgcac
    25801 acaaacttca aaggcttcgc aattgatgac ggcaattttc ggctggtcaa ttttattggc
    25861 aataaaataa tcggcggcag cgttacccag tttttaccaa attccagtgg atcgccgacc
    25921 agatacgccg agacatattt atcgacaccc ttttgattaa tacaggtgtt gtagcaaatc
    25981 accggaatac ccgcttcact ggcgcgacga acggtacggc tactgccatt ttcagacact
    26041 gccgataaaa taatggcatc gacattacgc gccacgaggg tatcaacaaa ggtactttct
    26101 ttcgaaatat ccccctgggc gttagtttca attaactgta cctgtactga agaatctttt
    26161 gccgcatcct gaacgccctg acgcactcca gcgtaatatc cctgggtatc aaggtatatt
    26221 gcaccaatgg tcatttcttt atccgcagcc ctggcaaata gtgcgctacc taatagcgta
    26281 gccattaata atagattacg ggttgttctc atttttttag gcatagtgcc tcctgtaggg
    26341 tttttattaa caacggctta ttctaattat tttgtgatga gcggcagcgc gtgccgctca
    26401 aatattacag ttaatagggt ttagtgaacg tagttaaaaa taaaaggcat cacagtggtg
    26461 aataatgccg ctttcggcca ggccggatta atcgccgtca attcagcgcg agcagactca
    26521 tattgcgcaa cctccccgag tcccgcggac gccagcatcg ccaccatcag gcatttttct
    26581 ttatgctgct gttgtaaatc gccatacagc gataacaggt caggttgtga aacagcaaag
    26641 aaatccgcct caatactggt tttcgccatc tcttgcgccc actgtttcat ttcactaaac
    26701 agttgctgtg cggtttgctg ttcacccagc agtcgcagcg ccattccttg ccagaagaga
    26761 taatcaaccg gctgatcgtt gtaataactg tggatgttaa tggtgcgatc gccggtcgcc
    26821 gccagacgta aacaacgcat cgcttcagtt tcatcgccct gcgcgttggc gcatatcgcc
    26881 tgccagaacc agatgtcgtt atcagtttgc cccggtaaac ggccttcgct taaattctcc
    26941 ggataataca gcgcggcatg aagcagttcg ctggcctgct gcggctctct ggcatcaaga
    27001 tgctgccagg cgcgtaataa ctggttgagg ataaactgac tggtgacctt cccttccccg
    27061 ccttcccacg ggtggaattt acgcgtggcg agaatgtccg ccgctttgtc tgcctgaccc
    27121 gtgagatgcc acaaattgag cagttctgcg gtcatgtcgt cgcgtttcag cgcaatttcc
    27181 agattatttt ccagacgcgc cagtcgtttc tccggtgtgg ctccacttaa cttatccagc
    27241 aaatcccgtt cgaaaagcag acgtgcatcc tgcggcgcaa gctgataagc attatcaaga
    27301 taacgcgcgg ccagctcata atcgtgtttc ttattccacg catggatcgc taacccgcgc
    27361 cagccgtcgg caaactccgg cgacatctct acgcaacgtt gccataaggc aatggctttg
    27421 ttgtagctac gtttgttgta gtagaagcag gccagtaaat ggcgagcaaa ccaacactct
    27481 tcaatactct ccagcgccgc cacttcttcc agcgtattcg ggaaacggac aaactgcggg
    27541 aagacatcaa tggcttttgc gaccagttcg ccacgttcgg ctttcggcag caagctggct
    27601 tgcaggtaaa gcggcagcgt gcgctggcag tccagtgcgt tcagcatctc tgccgccagg
    27661 gtgggcattc cccagttaat cagttgcccg gcggtcatca gagcgttaac gtcgcgtccc
    27721 tgacacagac cgcgccactg cgcgagggct gattcgctac gaccatcgaa ccagttcagc
    27781 caccacagag tggcgttcag cggataatca cgcagcagtt tctcgcgctg caaacgcgcg
    27841 ttgtcctgac gaccacttaa caccagcagc agattatgca agcaaagcac ttcctgattg
    27901 gttgggcagg cacgaagact ttgttggcaa aaatccagac ccgcgtcgaa gttatcatta
    27961 cgcgccgcca gtcgtgccag accataatag ccgcctgctt tactattgcc gctccagacc
    28021 gcacgccaga aatcctcttc ggcttgttga tattgtccct gacgttcgta agcactggcg
    28081 cgaatcaaac ttgcttgtcc acactgcggt tttttgttca gcgcatgtgc acgtttcagc
    28141 gcctgactgg catacgccac cgcttgcggg aaatctgcgc ggttatattc cagcatcgcc
    28201 agcgccaagt tacagcgata atccagcggg tccagcgcca cgccgcgcag gtagtaatcg
    28261 aacggtgaac ggctggcgtg atgatattgc tccagatgct gaccgataaa ccaggcttca
    28321 tctgtactgg taatgtcttg tgctgccagt ggcgctttgg cgacgtccgg caacggcaac
    28381 tcctgcggct gatgttcctg ataactcagt acaatattgc cgtcggcatc ggagagttca
    28441 atggtcagtc tgtcaggatt gataccgtgc aacacgccct ggatggcggt cgctggtgtc
    28501 agggccacgg catcatcgag caacgcgttg catttgccga tttcgcggat cgccagacgg
    28561 tatccgttca acggagagat ggcatacagc ccccactcaa tcccccgctc actacgctgg
    28621 agttttatca ccgcatcgcg ggaggcattt tgcaccatgc ccagagaatg ataaggcagg
    28681 aaatactgct cgaaacgctt ctcttcgtaa gcatcaagcc aggtaaaatc aggctggtta
    28741 tcggcaaaaa taccggtcat cagttcgata tacgggccgt tattgtcggt cagactctta
    28801 tcccacgcct ggccaaattc actgtgtccc caactccact gttttttacc tggcgcaata
    28861 tggtggttgg caacgtgcag caaaccgcca tcttcatcgt gacaccacgc gccgacaaaa
    28921 tcgtactgcg atttttcggc catatatgag gttggaacag gcacattttt atagcgagaa
    28981 atgtccactc cggcggagta gtccactttg tagtaagtgc cggtggcgat ggggaaagcg
    29041 gagacggccc gtttgccgtg atcaaacacc gcagtcacat ccggtgggaa aacgctctga
    29101 tgcccttcac cccctttcac tgccgggttg acccaccaca agaaatgacg cggcgtggcg
    29161 ttgccgttat agacgcggct ggcgatttcc agtgccgccc ggtcagggcg cagggtgaaa
    29221 cctgtcatca cctgtaaacc atgcatcggc tccgtttcgc cgacccacac cgtctgtgca
    29281 ccgtcttcat gggcttcgag ggtgaaatca acgggcataa aggtggtcgg gcgatggtgt
    29341 tgcggccagt taaactcaat cccgccggag atccacggtc ccagcatccc caccagcgca
    29401 ggtttaatga cttcattgtg atagacaaaa tcgcgctgtt tcactttatc ccatgcgcga
    29461 tgcacccgac cgcccagttc cggcaggatc atcactttga tgtagtcgtt ttccagccac
    29521 accgcctgct aggatttcag ggttttctgc tcgctcagcg tatcggtcac gccgtaggga
    29581 taaaccgcgc cggacgatcc ctgataaacg cgattttcca ggaacatggg atgtatatcc
    29641 tgcggcccgg tttcataggt cgggatctca acgcgctctt gccacacttt tactggagtc
    29701 atggtgccct caatattaac aagacatact gaattaaaag atttgtggca gtgtattgaa
    29761 caatctggca atgttttcgc ggaataatca cgcaattaac taaacaaggt ttagtgaaga
    29821 tgagagcctg cattaacaat caacagattc gccaccataa caaatgcgtg attctggaac
    29881 tgctgtaccg gcaaaagcgc gccaataaat caacgctggc ccggctggcg caaatttcga
    29941 ttccggcagt cagtaatatt ttgcaggaac tggaaagcga aaaacgggtg gtgaatattg
    30001 acgatgaaag ccagacgcgc gggcatagta gcggtacatg gctgattgcg ccggaaggtg
    30061 actggacgct gtgcctgaac gtgacgccca ccagtattga gtgtcaggtt gctaatgctt
    30121 gtttaagtcc gaaaggcgaa tttgagtatt tacagattga tgtaccgacg ccgcaggcgc
    30181 tgctgtccga aatcgaaaaa tgctggcatc gccaccgtaa attgtggccg gaccgcacca
    30241 tcaatctggc gctggcaatc cacgggcagg ttgatccggt gaccggcgta tcgcaaacca
    30301 tgccgcaagc gccgtggaca acgccagttg aggtgaagta tctgctggaa gagaagctgg
    30361 gcattcgggt gctggtcgat aatgactgcg tgatgctggc gctggcggag aaatggcaaa
    30421 ataattcgca ggaacgggat ttctgcgtga tcaacgttga ttacggcatt ggctcgtcgt
    30481 tcgtgattaa cgagcaaatt tatcgcggca gtttgtatgg tagcggacag attggtcaca
    30541 ccatcgttaa tccggatgga gtcgtctgcg actgtggacg ttacggcagc ctggaaaccg
    30601 tcgcctcgtt aagtgcatta aaaaaacagg cgcgggtatg gctaaaatca caaccggtta
    30661 atactcagct tgagcctgaa aaactgacaa ccgcgcagtt aatcgctgcc tggcagagtg
    30721 gagaaccgtg gatcaccagc tgggttgatc gctctgccaa tgccattggt ttgagtctgt
    30781 ataacttcct caacatcctc aatattaatc agatttggtt gtacggtcgc agttgtgcct
    30841 ttggtgagaa ctggcttaat actattattc gccagacagg atttaacccg ttcgaccgcg
    30901 acgaaggacc gagcgtgaaa gcgacgcaaa ttggcttcgg gcaattaagc cgcgcacaac
    30961 aggtgctggg aattggctat ttgtatgttg aggcgcagtt acgacagatt tgatggcgcg
    31021 ataacgtaga aaggcttccc gaaggaagcc ttgatgatca taaacgaaaa attgcctgat
    31081 gcgctacgct tatcaggcct acacggagat tgcaatatat tgaatttgca agattttgta
    31141 ggcctgagaa ggtgttcatg ccgcatccaa catgaacaac gagcacattg tcagcaaatc
    31201 accgtttcgc ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga
    31261 tgcgctcgat aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca
    31321 gttctttcgc ttcggcttct ttaaagccgc gacgggtaat cgccggagta cctacacgaa
    31381 taccggaggt cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga
    31441 tgttagcacg gcccagagcg gcgtctgctt ctttaccggt caggttttta tcaaccagat
    31501 caaccaggaa caggtggtta tcagtgccgc cggaaaccac tttgtagccg cgctcgagga
    31561 acacttctac catcgcttta gcgtttttcg cgacctgctg ctggtaagtt ttgaactcag
    31621 gctccatcgc ttctttcaga gcaaccgctt taccggcgat tacgtgcatc aacggaccgc
    31681 cctgaccacc agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt
    31741 tcgccaagat caggccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa
    31801 cgtgagcatg aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg
    31861 ccatatcaac gaacagataa gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt
    31921 caaccacgcc ggaatatgca gagaaaccac cgataatcat tttcggcttg tgttctttgg
    31981 cttgtttttc cagatcggcg taatcgatat gaccggtagc atcgataccg taaggaacga
    32041 tgttgtacag tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg
    32101 ccaggttcat acccagaacg gtatcacctg gttccagcag cgcggtgtag accgcaaagt
    32161 tagcctggga gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg
    32221 cacgatcgat cgccagttgt tcaacgatat caacatactc gcaaccgccg tagtagcgtt
    32281 tgcccggata accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc
    32341 gcgggctggt gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt
    32401 cctgctccat agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta
    32461 acatccgcat ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt
    32521 tggacaacgg cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat
    32581 ttacgcaaac gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg
    32641 gaaacggatt tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga
    32701 aaaacaccaa agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac
    32761 atcaattaag atgcaaaaaa ggaagaccat atgcttgacg ctcaaaccat cgctacagta
    32821 aaagccacca tccctttact ggtggaaacg gggccaaagt taaccgccca tttctacgac
    32881 cgtatgttta ctcataaccc agaactcaaa gaaattttta acatgagtaa ccagcgtaat
    32941 ggcgatcaac gtgaagccct gtttaacgct attgccgcct acgccagtaa tattgaaaac
    33001 ctgcctgcgc tgctgccagc ggtagaaaaa atcgcgcaga agcacaccag cttccagatc
    33061 aaaccggaac agtacaacat cgtcggtgaa cacctgttgg caacgctgga cgaaatgttc
    33121 agcccggggc aggaagtgct ggacgcgtgg ggtaaagcct atggtgtact ggctaatgta
    33181 tttatcaatc gcgaggcgga aatctataac gaaaacgcca gcaaagccgg tggttgggaa
    33241 ggtactcgcg atttccgcat tgtggctaaa acaccgcgca gcgcgcttat caccagcttc
    33301 gaactggagc cggtcgacgg tggcgcagtg gcagaatacc gtccggggca atatctcggc
    33361 gtctggctga agccggaagg tttcccgcat caggaaattc gtcagtactc tttgactcgc
    33421 aaaccggatg gcaaaggcta tcgtatcgcg gtgaaacgcg aagagggtgg gcaggtatcc
    33481 aactggttgc acaatcacgc caatgttggc gatgtcgtga aactggtcgc tccggcaggt
    33541 gatttcttta tggctgtcgc agatgacaca ccagtgacgt taatctctac cggtgttggt
    33601 caaacgccaa tgctggcaat gctcgacacg ctggcaaaag caggccacac agcacaagtg
    33661 aactggttcc atgcggcaga aaatggcgaa gttcacgcct ttgctgatga agttaaggaa
    33721 ctggggcagt cactgccgcg ctttaccgcg cacacctggt atcgtcagcc gagcgaagcc
    33781 gatcgcgcta aaggtcagtt tgatagcgaa ggtctgatgg atttgagcaa actggaaggt
    33841 gcgttcagcg atccgacaat gcagttctat ctctgcggcc cggttggctt catgcagttt
    33901 accgcgaaac agttagtgga tctgggcgtg aagcaggaaa acattcatta cgaatgcttt
    33961 ggcccgcata aggtgctgta atttgatgtt gccggatgga aacatccggc aacccttgac
    34021 gcggtttaaa ttgccgcgtc gtcctcttca ccggtacgga tgcgaatgac ccgagccacg
    34081 tcaaagacga agattttacc gtcaccgatt ttgccggttt gcgccgtgcg aataatggta
    34141 tcgacacagg tatcgacaat gtcgtccggt acgacaatct caattttcac tttcggcaga
    34201 aaatccacca tatactccgc gccgcggtac agctcggtat ggcctttctg gcgaccaaag
    34261 cctttcactt cggtcaccgt catgccggta ataccgactt cggccagtgc ttcgcggaca
    34321 tcgtccagct tgaagggttt tataatcgca tcaatctttt tcatgctatt ccttgaaaag
    34381 gttgcctgtc ttttgatctg ctaaacgtaa cacataacgc caattcattc cttgaaatcg
    34441 tttgcatcca gctcgtgtcg ggaaagcagt ttataaaatt ctgtccggtt gcgccccgcc
    34501 attctcgccg cgtgggtgac gttgcctttg gtgatttgca gcagcttacg caagtagttg
    34561 agttcaaaat gattacgtgc ctcaacaaag gttggcagcg ccgtattttc accctccagc
    34621 gcctgctcca ccagcgcatc actaatcacc ggagatgagg tcagcgccac gcactgttca
    34681 atcacgttga ccaactggcg cacattaccc ggccagctcg cggtcatcag gcgtttcatc
    34741 gcatcggtag agaacgcgcg gacaaacggt ttatgtcgct ctgccgcctg gcgcaacagg
    34801 tgatttgcca gtagcggaat gtcttctgtg cgctccgcca gtgccggaat tttcaggctg
    34861 acaacgttga ggcggtaata gagatcttcg cggaattccc cgcgcgccat cgcttttgac
    34921 agatcacggt gagtggcaga aataatccgc acattgatat caatatcgcg gttactgccc
    34981 agcgggcgca ctttacgctc ctgcaacacg cgcagcagtt tgacctgtaa cggcgcaggc
    35041 atatcgccaa tttcatcgag aaatagcgtt ccgccttccg ccgcctggaa taaaccttcg
    35101 cgattgctga cagcgccagt aaacgcgcca cgcgcatgac caaacagctc cgactccagc
    35161 aattgctcgg gcaatgcgcc acagttaata gcaataaatg gtttgctgtt gcgcgggctg
    35221 gcgttgtgga tagcctgggc gaaaatctct ttcccggtgc cgctctgacc gttaatcaaa
    35281 acgctgacgt ctgattgcgc caccagccgc gcctgttcca gcaaacgcag catcagcggg
    35341 ctgcgggtga caattgcctc gcgccagcgt tcatcggtgg ctggcgcgga ttgctccagc
    35401 gcatcgtcaa ttgcctgata tagcgcatct ttatcgacag gcttggtgag gaaactaaaa
    35461 acgccctgct gtgttgcagc aacggcatcg gggatagaac catgcgcagt aagaataatt
    35521 actggcattc ccggctgcac tttctggatt tcagcaaata gctgcatacc gtccatttca
    35581 tccatccgca ggtcgctgat aactaaatct actttttcgc gattcagtac ccgtaatcct
    35641 tcagcgccac tttccgccgt gaccacactg tagccttcgc tggtcaggcg caggccaagc
    35701 agtttcagca atcccggatc gtcatcgacc aataataaat gcgcaggttt atggctcatc
    35761 aggagtgacc tcatgggtgg atggcgcggg cttttcactc tcgtgcggcg tatccggcga
    35821 gaaatttcca gccggtttgc gggtcgagag ctggcgttca atatcggtca ggttttccag
    35881 cttgcgggtg gtgagttcca gttgctgctg tagaacgtga tgttgctggc gcaatgtatc
    35941 cagctcgctg tcgctggact gctggagttt actgtaacgt tggcgctctt ccgccagttg
    36001 cagttgcagc gcctgaccgt cgcgccagag ttgatacagt gggcgaacct gtgccgggat
    36061 ctcggtactt aacgcttcaa tacgcgcgac cagctggcgg cgctcatacg gcgtaatttt
    36121 ggcgtcggcg agcaaaatcc cttgtttaaa ggtattttgc cagctgccgt cgtcatattg
    36181 gcgagcttgc tgacgcgact gcgcaggcat taaacgatca gcacaatcca tcgcccgcag
    36241 ccagtaaagc ggattggttt cggttgattt accttgcagc gcccagatgt cgctacattc
    36301 agtagaaaga tagtcagcca gttgataaac cggaattttt tcttctgctg gcgtatcaat
    36361 ggctggctta ttgtgattct gcacgcaacc cagcaatgcc agacatggca gccctgccag
    36421 ccacagccgt cggggcaata atcgttgaaa aatgtgtcgc atattcacca gacttaaagc
    36481 ctatcccagt gggcgtaatt gttgcagaca gtctggacat ggacagcgcg gagaaaccgg
    36541 ggcgtacata tcgtacgtga agatttcgag cactacccgg ggccgaactg acaaataaaa
    36601 tagcctgata ggataggctg ttagcattat ttcgtgtttt tcgacgacgg taattcaatg
    36661 cggaaacaaa cgtcttgccc gctctcgtcg accagataca gttccccttg catacggcga
    36721 atacaatccc tggcaatgct taatcccaga ccgctgccct tcaccgcccc ttttcgctgg
    36781 tggcttccct gaaaaaaggg ttcgaagatc atggcgcgtt cctcttgcgg aatgggcgtg
    36841 cctgtattga tgacatcaat ataaacccgc gcaccatgta aactgctgcg aaggcaaatg
    36901 ttaccggatt cagccccgta gtgcaccgca ttggagtaaa gattatccag tacgctcatc
    36961 agcagcattg gctccgccag gcaagctgtt gctttgagat cgacgtcggt atgcatcatt
    37021 ttagcccgtg cgggcaggct atgagcagaa accactgtct ccaccagcgg tgctaattca
    37081 acattctcca gttccaccgc actgtccgcc tgtttacggt tgtaatcaag cagttgttcg
    37141 atcagttttt gcaaattgca gctgctgcta tcaagaatgc tcaccacctc tttttgctct
    37201 ggcgtaagcg gcccgacaac ctgatcagcc agtaattcag tgccctcgcg catactcgcc
    37261 agtggcgttt ttaattcatg agataaatgt cttaaaaatt gatggcgttg ggattccagc
    37321 catgacaggc gctcacttaa ccaaagaata cgttgcccaa ccgagcgtaa ctcgctcggt
    37381 ccacagaacg aaacgctatt gcccagagaa cgcccttccc ccagacggtt gatcatgcgc
    37441 tcgatatttt tcaccggccc gataatcatc cgcgtgaaaa gtagcaccat caccagactc
    37501 accagaaata gcaccagcga ttgccaacca aaatattgcc cacgttcggc gatttcacgc
    37561 tgaagttgct gcccacgaga gaacaccact gtgcgcgtgg cctgcaccat ttcggtattg
    37621 gcactggcaa aggcttcaag acgcgcggcg gcggcagcat cgggaccact gttgttacac
    37681 tgaagttggg ccagattgtt caaatcctga cgcaatgcct ggtaaagttt atcgtctggc
    37741 agtacgcctg cgtgggcatc gagcatttcg ctgtaacgct tgcgctggct ttgataaacc
    37801 ttcgcaagcg tagggtcgtc cagcacgcaa tactgacggt agctacgctc catctccagc
    37861 gccgcgttgg tcatcgcttc actacgccgg gcatcaataa gcgtagtgcg gttaaccagc
    37921 gccgcctgat cgctaagcgc attcaggctt tgccaggctt gccatgccag caccaacagg
    37981 ggtagtagaa ttagcaaaaa tgccagcatt accagttgtc gtaatgagcg gggaaaaacg
    38041 ggccagcgtt tcaaggtgtt actctcgtca gacgcgaata gcctgatgct aaccgagggg
    38101 aagttcagat acaacaaagc cgggaattac ccggctttgt tatggaataa ggcggtgcct
    38161 aactcgacgt ttcgcccgat ggttgatata gctacgctga tatcagaagt tggacggcag
    38221 gcaccttgtt gtgcgtcatt cgaattttat gtagcacgtc ccgaaggggc tgacataagt
    38281 cggtgaatga gccactgctt actattatgc agtaactgtg ccaataaaga aaatagtttg
    38341 gtaacgtact gattatacgt tgctttgagg gatttatggc ttcttcgcta tttgaatgta
    38401 aatcagccac tgtgtcgcta aaaagagaca acttaagata aacttattag attacaataa
    38461 aatcaatcat ttaagtgtcg ccagaaagcg acacggtaaa ccacccattg tcgtgattta
    38521 cagacacaaa aaagctcccg gagttgggag cttatgatag tggttggtgc ttaatgccgc
    38581 atccgggctg caaaaccaat gaactgatga cttaccccaa ctgcttacgc gcattgcgga
    38641 aaatgcgcat ccatgggcca tcctcgcccc agttttccgg atgccaggag ttgctgacag
    38701 tacggaaaac acgttccggg tgcggcatca taatggtgac tcgaccactt tcagtcgtaa
    38761 ccgccgtaat accgttcggt gaaccgttcg ggttagccgg gtaggtttca gtgactttgc
    38821 cgaagttatc gacatagcgc agtgccacca gccctttgct ttccagtgcc gccagatgcg
    38881 ccgcatcacg cacttccacg cgcccttcac catgagagac agcaatcggc atttgcgagc
    38941 ccaccatccc ctgcaacagc agagacgggc tttgggttac ttcaaccagg ctgaaacgcg
    39001 cttcaaagcg atcggaggtg ttacgcacaa aacgtggcca caactcactc cccgggatca
    39061 gttcacgcag gttagacatc atctggcaac cgttacatac ccccagggcc agcgtttgcg
    39121 gacgatggaa gaaggttgca aactcatcgc gtacacggtc attgaacagg atagacttcg
    39181 cccaaccttc accggcaccc agcacatcac cgtaggagaa acctccgcac gcgaccaggg
    39241 cgtgaaaatc ctccagtccc gtacgtccag tcagcaggtc gctcatatgc acgtcgatag
    39301 catcaaagcc cccacgttgg aaagctgccg ccatttcaac atgcgagttc acgccctgct
    39361 cacgcagtac ggcaactttc ggacgtgcgc cagtggcaat atacggtgct gccacatctt
    39421 cgttgatatc gaacgacagt tttacattca ggcccggatc ggcgtcgtta gatttcgcct
    39481 gatgctcctg atcggcacac tccgggttgt cacgcaggcg ctgcatctgc caggtagttt
    39541 ctgcccacca gacacgcaac gtagtgcggc tttcgctgaa tacagtctgc ccgttggcgg
    39601 taatcacaaa acggtcaccg gaaaccgcct gcccaacata atggacgcaa tccgcaagcc
    39661 catgctgtgc cagtacggac tcgaccgctt cacggtcagc ggcacgaacc tgaatcaccg
    39721 cacccagttc ttcgttaaac aacgccgcca ggcgatcgtc acccagagag gcgatatccg
    39781 cgtcaatgcc acaatgacct gcaaaggcca tttccgccag cgttaccagc aggcctccat
    39841 cagagcggtc gtgatacgcc agcagcttac gctgtgcaac cagcgcctga atcgcgtcat
    39901 agaagccttt cagttggaca acatcgcgta catctgccgg tttgtcgcca agctgacgat
    39961 aaacctgcgc cagcgccgtt gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca
    40021 gcagtgcgtt atcttcggta caaagctgcg gcgtgatggt gtgacgtaca tcttccacgc
    40081 gggcaaatgc agaaatcacc agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt
    40141 cctgccagcg ggttttcatc gacatagagt ctttacccac cgggatcgtc aggcccagcg
    40201 ccggacaaag ttcttcaccc acggctttaa cggcttcata caggcccgca tcttcaccag
    40261 ggtggcctgc cgccgccatc cagttggcgg aaagtttaat gcgtttgata tcgccaatct
    40321 gtgttgcggc aatattggtt aacgcttcac cgaccgccag acgggcagag gcggcgaaat
    40381 ccagcagcgc aaccggcgca cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga
    40441 ggctggcggt agtgaccgcg cagttagcga ccggcacctg ccacggcccc accatctgat
    40501 cgcgcgccac catgccggtt acgctgcggt caccgatggt caccaggaaa gttttctccg
    40561 ccacggtcgg cagatgcagt acgcgtttca ccgcgtctgc aatggtgatc ccttcacgga
    40621 ccagcgcatc gcccttcgct ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac
    40681 caagcaggac gtccagcggc agatcgatcg gctgattatc aaaatgacga tcgtgcagag
    40741 aaagatgcag ttcttcggtc gcttcaccaa tcaccgcgta gggtgcgcgc tcacgcttac
    40801 acagttcgtc aaacagcggt aattgatcgg cagcaaccgc cagcacgtag cgttcctggg
    40861 attcgttaca ccagatttcc agcgggctca tgcccggttc gtcgcttaga atatcgcgca
    40921 gttcaaattt accgccgcgt ccgccgtcgc tcaccagctc cggcatcgcg ttagaaagac
    40981 cgcccgcacc gacgtcgtgg ataaacagaa ttgggttagc atcaccaagc tgccagcaac
    41041 ggtcgatcac ttcctggcaa cgacgctcca tctccgggtt gtcgcgctgt acggaagcaa
    41101 agtcgaggtc ggcatcagac tgaccagacg ccatggaaga cgccgcgccg ccgccaagac
    41161 cgatgttcat tgccgggccg ccgagaacga ccagcttcgc accgacgttg atctcgcctt
    41221 tttgtacgtg atcggcgcga atgttgccga tcccgcccgc cagcatgatc ggtttgtgat
    41281 aaccgcgcag ctcttcgcca ttgtggctgt tcactttttc ttcataagta cggaagtagc
    41341 cgttcagtgc cggacgacca aattcgttgt taaacgccgc accgcccagc gggccttcgg
    41401 tcatgatgtc cagcgcggta acaatgcgct caggcttacc gaaatcttct tcccacggct
    41461 gttcgaagcc aggaattcgc aggttggata cggagaaacc aaccaaacca gctttcggct
    41521 tcgcgccgcg cccggtggca ccttcatcgc ggatttcacc gccggaaccg gtcgccgcgc
    41581 ccggccacgg agaaatcgcc gtcgggtggt tgtgagtttc gactttcatc aggatgtgcg
    41641 ccggttcctg atggaaattg tagcggcccg tttcgtggtc agcaaagtag cggcccactt
    41701 cagaaccttc cattacggcg gcgttatctt tataagcaga gagaacgtga tctggcgtgg
    41761 tttcgaaagt atttttgatc atcctgaaca gcgatttcgg ctgttgttca ccgtcgataa
    41821 tccagtcggc gttaaaaatt ttgtggcggc agtgctcaga gttcgcctga gcgaacatat
    41881 acagctcgat gtcgttcggg ttacgaccaa gctttgtgaa agcatcctgc agatagtcaa
    41941 tttcatcttc cgccagagcc aagccaagac gcaggttagc gtcgatcagc gcctgacggc
    42001 cctgccccag caaatcaacg cttgtgaccg gagtcggttg atggtgggca aacaactgct
    42061 ctgcatcatc taaatcaaaa aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtaa
    42121 cctgctgcca ttgttcattg gtcagcgtac cggcttctat atagtaagca acgccgcgct
    42181 caaggcggtt tacctgttgt agcccgcagt tatgggcaat atcggtcgct ttcgaagacc
    42241 agggagagat ggtgccagga cgcggggtca ccagcaggag tttgccttgc ggggcgtggc
    42301 tggcgagtgc cggaccatat ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta
    42361 acggcgcatt gaggtcagca aaatggacat actcggcgta aatattgtga accgggagcc
    42421 tggcagcctg aaaacgtgcc agcagtttgt tgattcggaa tgccgacagt gcaggcgaac
    42481 cacgcagaat ttccatcata agtctctcgt cttctaagct ttcggtgtac ccaagggggg
    42541 ggaaacgggc gtcattataa agaatctgat gcgctgacga aaccgtttgc gtggaaataa
    42601 aatcaccatc gtgaattagc aacgcgtgcc gccaatggct gtaataagtt gccatctggc
    42661 gcaggtttac gcaaaatgcc gctcatttat gagtaaacct ttcactatta ttacgttttt
    42721 tcaagctggg acgcgcacga cacagagaat taactaattg aaaaaattaa agattaatta
    42781 tctgttcatc ggcattctgg cactgctgct cgcggtcgct ctctggccat ccattccctg
    42841 gtttggtaaa gccgacaacc gtatcgccgc cattcaagcg cggggagagt tgcgtgtgag
    42901 caccattcat actcccctga cttataacga aatcaacggg aaaccttttg gcctggatta
    42961 cgaactggcg aaacagtttg ccgattacct cggcgtaaaa ctgaaagtga ccgtgcggca
    43021 gaatatcagc cagctgtttg acgaccttga taatggtaac gccgacctgc tggcggcagg
    43081 actggtctat aacagtgagc gggtaaaaaa ttatcagcct ggccctacct attattccgt
    43141 gtcacaacaa ctggtttata aagtgggtca gtatcgccca cgtacgctgg gcaacctgac
    43201 ggcggagcaa ctcaccgttg caccgggtca tgtggtagtt aacgatctcc agaccctgaa
    43261 agaaacaaaa ttcccggaat taagctggaa ggtagacgac aaaaaaggct ctgcggaatt
    43321 aatggaagat gtcatcgaag gaaaactcga ttacaccatt gctgattctg tcgccatcag
    43381 cctgtttcag cgcgttcacc cggagctcgc cgtagcgctc gatatcaccg atgaacaacc
    43441 ggtgacttgg tttagcccgt tagatggcga taataccctt tccgccgccc tgctcgactt
    43501 cttcaacgaa atgaatgaag acggtacgct ggcacgcatt gaagagaaat acctggggca
    43561 tggcgatgat tttgattacg tcgatacgcg cacattttta cgcgccgtcg atgcggtact
    43621 gccgcagtta aagcccctgt ttgagaaata cgccgaagaa attgactggc gtttgctggc
    43681 cgctattgct tatcaggaat cgcactggga tgcacaggcc acttcaccga cgggtgtgcg
    43741 cggcatgatg atgttaacca aaaataccgc gcaaagcctc ggcattacgg atcgtaccga
    43801 tgccgaacag agcatcagcg gtggcgtgcg ttatttgcag gatatgatga gtaaagtgcc
    43861 ggaaagtgtg ccggagaacg aacggatctg gtttgccctc gctgcgtaca atatgggcta
    43921 tgcgcatatg ctggatgccc gcgccctgac ggcaaaaacc aaagggaatc ctgacagttg
    43981 ggctgacgta aaacagcgtc tgcctttact tagccagaaa ccctattaca gcaagctgac
    44041 ttacggctac gctcgtgggc atgaagccta cgcttatgtc gaaaatattc gtaagtatca
    44101 gattagcctg gtgggttatc tgcaagagaa agagaagcag gctacagaag cggcgatgca
    44161 actggcgcag gattatccgg cggtatcgcc tacggagttg ggcaaagaga aatttccttt
    44221 tctctcgttt ctttcccagt cgtcatcaaa ctatttgacc cattctccct ctctgctgtt
    44281 ttccaggaaa gggagtgaag agaaacaaaa ttaatccgtc gaggattgcg cttttttctg
    44341 cgctttaatt tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg cgcactcatc
    44401 cgccagtatt ccttccgtaa tttccactcg gtgattcata cccggatgat gcagcacatc
    44461 cattaaagat cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca cgcgaccaat
    44521 gcgactgtgg atcatcgctc cggcacacat tacacatggt tcaagcgtga catacaacgt
    44581 ggcgtcgatc agacgataat tttgcatcac cagaccaccc tgccgcaggg ccatgatttc
    44641 tgcatgtgcg gtgggatcat ggcgaccaat cgggcggttc cagccttcgc cgattacccg
    44701 attgttatgc actaataccg cgccgaccgg cacttcccgc tcatcccagg cacgtttcgc
    44761 cagcgtcagc gcgtgacgca tccaatattc gtggctaaat tcgacttcag acaaaaagaa
    44821 aactccggtt ataaaagcgc ggcgcattat acacggacgc tatgctttac tctattccag
    44881 ttgctggagt tcaccgcgcg gggttactcg ccaacgatgc tgacagaaat aaagcagcgg
    44941 attgtcctga ttactgtcgc tatagccact gtacagccgc agcggcgtgc cgattttgcg
    45001 ctccagttgt gcgacctttt catgtcccag acaacgcatc gtcaataccc aaccaccata
    45061 gccacgctga atttggctgg cgataagatt aacccgcggc agccagggcg tatcgaaata
    45121 aaccgcttca accagcggct gcggagagcc ggtaatcaac cagatatcag catcggaact
    45181 taacaggtag gtggttaatc gctcctgaac cagcggaaag gcggtaacat tgtcgcgaaa
    45241 ccagcgcacg aaatcggcct gcaacgtctg taaacgtgct tcgctgtgac caaaagtgca
    45301 cccccacaga agcagactca tcggccagcg tgccgcacga ccttttatca ataacgcaat
    45361 ggctataatc ggtaacaacg gcaggacaag taacgcattc agcggttggc gacgcagtaa
    45421 atagcgcaga aaactgccga acatatcctg ctgatgcaat gttccatcta agtcaaaaaa
    45481 caccacacga cgctcgtgag ttgccaaacc atactcctct ggactgaaac atcctgatta
    45541 attttcgcta ctacatagcc taacagatag atcatcactt ttccggcaac aatcgaccgc
    45601 atcatggcta actggaattt ttaattcatg gcaattagcg gcaatggaat ataaaattca
    45661 ctcgcgtgtg tctcatattg cctgttgtcg ccactatttc atcgcgagga aagatatgaa
    45721 cggcttactt cgtatccgtc agcgttacca agggcttgcc cagagtgata aaaaactggc
    45781 ggattatctg ctgctacaac ctgatacggc gcgccattta agctctcagc aactggccaa
    45841 cgaagccgga gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg gctataaagg
    45901 ttttccggcg cttaagttgg cgttgagtga agcgctggca agccagccgg aatcgccctc
    45961 cgtgcccatt cataaccaaa tccgcggtga tgatccgtta cggctggtcg gcgaaaaact
    46021 gattaaagaa aataccgccg ctatgtacgc aacgctaaac gttaatagtg aagagaaact
    46081 gcatgaatgc gtaacaatgt tgcgctctgc gcggcggata attctgaccg gtattggcgc
    46141 ttcgggtctg gtggcgcaaa actttgcctg gaagctgatg aagattggct tcaatgctgc
    46201 cgcactgcgc gatatgcatg cgctactcgc aacagtacag gcgtcgtccc ctgacgatct
    46261 gttattagcc atttcctaca ccggtgtacg acgcgagtta aacctggcgg cagatgagat
    46321 gctgcgagtg ggcggaaaag tgctggcgat taccggcttt actccgaatg ccctgcaaca
    46381 gcgtgcttct cattgcctgt ataccattgc cgaagaacaa gcgacaaaca gtgcttcaat
    46441 ctctgcttgt cacgctcagg gaatgttaac ggatttgctg ttcattgcgc tgattcagca
    46501 ggatctggaa ctggcaccag aacgtattcg tcatagtgaa gcgctggtga aaaaactggt
    46561 ctgagtaaag aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag agtttcctta
    46621 tggcgctgtt aatcactaaa aaatgcatca attgtgatat gtgtgaaatc aaataaaaac
    46681 acatttattt tcatagtgat acagcaatgt gttattgaat gtgttgttgg atttatggtg
    46741 caaaaaaatt ttgcataagt tcagcaacac aaagaacacc actttcttta acgtcctccg
    46801 cgccctaaag gacggggctt tacggcgcaa aaggcctgtg acatacaaca ttaacttttc
    46861 agtcaaccgt agcacgttct tgtatacgcc gtgtctgcgg ttttatataa ccctggctgg
    46921 aaacctctta tacaaagtcg attcgccaac atcatagatg atcgccacct tctggcgaga
    46981 aactcctgat gcaattagtc gcccggcctg cgcccattat tctggtgaaa gtttgggacg
    47041 gcgaccgcca attcgtccct gtgcgcgagc agcttccagt cctgcttttg ttcgttcaac
    47101 aatcagttca cgttccattt cagccagggc acccatcaca tggaagaaaa agcgtcccat
    47161 tggtgtgctg gtatcaattg aatcagtcag actacgaaag ttgatgcctc gttcgcgcaa
    47221 cgcctccacc agcacgacaa gatgccgcat actgcgcccc agccgatcca gcttccagac
    47281 aaccagtgtg tcgcctgccg ataatgtctt gagcagtttt ttcagccccg gtctttcgga
    47341 ctttgtacca cttatcttgt cttcaaaaat cagctcgcat cctgcacagt tcaacgcatt
    47401 acgttgtaga tcggtattct ggtcatttgt tgatacgc
//
LOCUS       DAJTPG010000021        46339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTPG010000021 DAJTPG010000000
VERSION     DAJTPG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(193..268)
                     /locus_tag="OO230_001239"
     tRNA            complement(193..268)
                     /locus_tag="OO230_001239"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Lys,seq:ttt)
     gene            complement(433..1224)
                     /gene="cpoB"
                     /locus_tag="OO230_001240"
     CDS             complement(433..1224)
                     /gene="cpoB"
                     /locus_tag="OO230_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5691070.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1234..1755)
                     /gene="pal"
                     /locus_tag="OO230_001241"
     CDS             complement(1234..1755)
                     /gene="pal"
                     /locus_tag="OO230_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5691071.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1790..3082)
                     /gene="tolB"
                     /locus_tag="OO230_001242"
     CDS             complement(1790..3082)
                     /gene="tolB"
                     /locus_tag="OO230_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5691072.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3215..4456)
                     /gene="tolA"
                     /locus_tag="OO230_001243"
     CDS             complement(3215..4456)
                     /gene="tolA"
                     /locus_tag="OO230_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5691073.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4521..4949)
                     /gene="tolR"
                     /locus_tag="OO230_001244"
     CDS             complement(4521..4949)
                     /gene="tolR"
                     /locus_tag="OO230_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5691074.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4953..5645)
                     /gene="tolQ"
                     /locus_tag="OO230_001245"
     CDS             complement(4953..5645)
                     /gene="tolQ"
                     /locus_tag="OO230_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5691075.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5642..6046)
                     /gene="ybgC"
                     /locus_tag="OO230_001246"
     CDS             complement(5642..6046)
                     /gene="ybgC"
                     /locus_tag="OO230_001246"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5691076.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6196..6489)
                     /gene="ybgE"
                     /locus_tag="OO230_001247"
     CDS             complement(6196..6489)
                     /gene="ybgE"
                     /locus_tag="OO230_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5691077.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6489..6602)
                     /gene="cydX"
                     /locus_tag="OO230_001248"
     CDS             complement(6489..6602)
                     /gene="cydX"
                     /locus_tag="OO230_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5691078.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6617..7756)
                     /gene="cydB"
                     /locus_tag="OO230_001249"
     CDS             complement(6617..7756)
                     /gene="cydB"
                     /locus_tag="OO230_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5691079.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7772..9340)
                     /gene="cydA"
                     /locus_tag="OO230_001250"
     CDS             complement(7772..9340)
                     /gene="cydA"
                     /locus_tag="OO230_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5691080.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10189..12822)
                     /gene="mngB"
                     /locus_tag="OO230_001251"
     CDS             complement(10189..12822)
                     /gene="mngB"
                     /locus_tag="OO230_001251"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5691081.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12840..14756)
                     /gene="mngA"
                     /locus_tag="OO230_001252"
     CDS             complement(12840..14756)
                     /gene="mngA"
                     /locus_tag="OO230_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5691082.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14926..15648
                     /gene="mngR"
                     /locus_tag="OO230_001253"
     CDS             14926..15648
                     /gene="mngR"
                     /locus_tag="OO230_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5691083.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15751..16620)
                     /gene="sucD"
                     /locus_tag="OO230_001254"
     CDS             complement(15751..16620)
                     /gene="sucD"
                     /locus_tag="OO230_001254"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5691084.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16620..17786)
                     /gene="sucC"
                     /locus_tag="OO230_001255"
     CDS             complement(16620..17786)
                     /gene="sucC"
                     /locus_tag="OO230_001255"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5691085.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17880..19097)
                     /gene="odhB"
                     /locus_tag="OO230_001256"
     CDS             complement(17880..19097)
                     /gene="odhB"
                     /locus_tag="OO230_001256"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5691086.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19112..21913)
                     /gene="sucA"
                     /locus_tag="OO230_001257"
     CDS             complement(19112..21913)
                     /gene="sucA"
                     /locus_tag="OO230_001257"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5691087.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22214..22930)
                     /gene="sdhB"
                     /locus_tag="OO230_001258"
     CDS             complement(22214..22930)
                     /gene="sdhB"
                     /locus_tag="OO230_001258"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5691088.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22946..24712)
                     /gene="sdhA"
                     /locus_tag="OO230_001259"
     CDS             complement(22946..24712)
                     /gene="sdhA"
                     /locus_tag="OO230_001259"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5691089.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24712..25059)
                     /gene="sdhD"
                     /locus_tag="OO230_001260"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24712..25059)
                     /gene="sdhD"
                     /locus_tag="OO230_001260"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5691090.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25053..25457)
                     /gene="sdhC"
                     /locus_tag="OO230_001261"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25053..25457)
                     /gene="sdhC"
                     /locus_tag="OO230_001261"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5691091.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25838..25945
                     /locus_tag="OO230_001262"
     CDS             25838..25945
                     /locus_tag="OO230_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691092.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26151..27434
                     /gene="gltA"
                     /locus_tag="OO230_001263"
     CDS             26151..27434
                     /gene="gltA"
                     /locus_tag="OO230_001263"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5691093.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27825..28391
                     /locus_tag="OO230_001264"
     CDS             27825..28391
                     /locus_tag="OO230_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5691094.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28442..30901
                     /locus_tag="OO230_001265"
     CDS             28442..30901
                     /locus_tag="OO230_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5691095.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30916..31647
                     /locus_tag="OO230_001266"
     CDS             30916..31647
                     /locus_tag="OO230_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5691096.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31644..32720
                     /locus_tag="OO230_001267"
                     /pseudo
     CDS             31644..32720
                     /locus_tag="OO230_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32872..33918
                     /locus_tag="OO230_001268"
     CDS             32872..33918
                     /locus_tag="OO230_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5691097.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33915..34706)
                     /gene="nei"
                     /locus_tag="OO230_001269"
     CDS             complement(33915..34706)
                     /gene="nei"
                     /locus_tag="OO230_001269"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5691098.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34742..35476)
                     /gene="pxpA"
                     /locus_tag="OO230_001270"
     CDS             complement(34742..35476)
                     /gene="pxpA"
                     /locus_tag="OO230_001270"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5691099.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35466..36398)
                     /gene="pxpC"
                     /locus_tag="OO230_001271"
                     /pseudo
     CDS             complement(35466..36398)
                     /gene="pxpC"
                     /locus_tag="OO230_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36392..37048)
                     /gene="pxpB"
                     /locus_tag="OO230_001272"
     CDS             complement(36392..37048)
                     /gene="pxpB"
                     /locus_tag="OO230_001272"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5691100.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37071..37814)
                     /gene="ybgI"
                     /locus_tag="OO230_001273"
     CDS             complement(37071..37814)
                     /gene="ybgI"
                     /locus_tag="OO230_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5691101.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38085..39566
                     /gene="dtpD"
                     /locus_tag="OO230_001274"
     CDS             38085..39566
                     /gene="dtpD"
                     /locus_tag="OO230_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5691102.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39716..41134)
                     /gene="phrB"
                     /locus_tag="OO230_001275"
     CDS             complement(39716..41134)
                     /gene="phrB"
                     /locus_tag="OO230_001275"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5691103.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41131..41640)
                     /locus_tag="OO230_001276"
     CDS             complement(41131..41640)
                     /locus_tag="OO230_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5691104.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41769..42056
                     /locus_tag="OO230_001277"
     CDS             41769..42056
                     /locus_tag="OO230_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5691105.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42111..42491)
                     /locus_tag="OO230_001278"
     CDS             complement(42111..42491)
                     /locus_tag="OO230_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691106.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42494..45880)
                     /locus_tag="OO230_001279"
     CDS             complement(42494..45880)
                     /locus_tag="OO230_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5691107.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(45899..46102)
                     /locus_tag="OO230_001280"
                     /pseudo
     CDS             complement(45899..46102)
                     /locus_tag="OO230_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11123 a        12625 c        11197 g        11394 t
ORIGIN      
        1 cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta atcacccgac
       61 actacgctgg atactacaac taactatgtt agtgggggtc gtgcaggatg actcgacttc
      121 gtctcgccct tcggcgccgt tgctgatgca acgttatcct tcacgtttta ctaccgcttc
      181 caccttagcg attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
      241 actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt gtttgatatc
      301 ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa aatttcgatg
      361 cagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg acttctggtc
      421 gtgtgttatg cattacatcg cgttcagacg tttttgtgcc tgtttagcgc catcagtacc
      481 agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt cacctttgtc
      541 ctgcatgatg acgccgactt taaacatcgc atcagcagcc tttggtgact tcggatagtt
      601 tttcactacc gaagcaaaat agtacgccgc atcatctttt ttacccttgt tgtagtttaa
      661 ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa
      721 attctgaaat gccaccattg cgtcatcctg gcgggattta tcctgcacca gcgcaatagc
      781 tgcattgtaa tccgtgtttg catcgccgct tttcaccggc gcgccagcat tcgcagtacc
      841 agcatcagct gtcggcgtcg ttgatgccgt cgcaccgctt tgatcgctgc tggttgattg
      901 cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac aggatctgct tttgccgctc
      961 cacgacctga ttcagttgat actgattttc ctgaatctga ccacgcaggg aatcaatatc
     1021 ggattgatta tcagaaagtt gttgctggag ttgggttaaa agctggctgt gagcattaga
     1081 aatacgctca agttgagtga cgcggtcttc gaccgagcct gagctgacac tactgattgg
     1141 tgcctgagca aaagcggccc agggggccgc tataccaacc agtaacgaca gactcaatag
     1201 ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg cacgacggtt
     1261 tttggagtat gccgcttcgt catgacccag tactgcaggt ttttctttac cgtaagaaac
     1321 gatggagatc tggtctgcag aaacgccttt accctgcagg tacatcttaa cggcgttcgc
     1381 acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg cgtgaccttc
     1441 tacggtgact ttgtaagacg ggttgctacg caggaagttt gcatgtgcat ccagcatttg
     1501 agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt tgttctgctg
     1561 cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt tgccgccgtt
     1621 cgcatccata ccagtgccgg cacccagcat gccttcgctg ccgtcattgc tggcgttctt
     1681 gttggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc ctttcagcac
     1741 tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat cacagatacg
     1801 gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct ttgaaacgcc
     1861 catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg tagattacca
     1921 tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac agaacttgta
     1981 cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccattg gagctgacca
     2041 ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg
     2101 taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgcgga cgaccggcct
     2161 ggtcagaagt aaatgccagg ttctggctat ccgggaacca ggtcggttcg gtattgttac
     2221 tgcgaccatc agtcacctgg cggatctgac cagaagccaa atccattacg tacaggttca
     2281 gactaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg
     2341 caccgttgtg acgcgggaat gaagccacct gacgtacagc gccatttgcc agcgtctgaa
     2401 taaccagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa ccgtctggtg
     2461 accacgccgg agacatcagc ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt
     2521 catagtcaga tacgcgcagt tcatacggga actgaccgcc gttggtctga acaacgtagg
     2581 caatacgggt acggaacgca cctttaatgc cggtcagttt ttcaaacact tcatcactgg
     2641 cggtatgacc agcataacgc agccactgct tgttcacttt gtacgagttc tgagcaagta
     2701 cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa gaaccatccg
     2761 gattcggagt gacttgaccg acaactacag cgtcaatgcc cagtgcggac catgcagctg
     2821 gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcgcgatct aacggattaa
     2881 atttaccgct gttacgcaag tcagcagcaa cgatgccgcc aatatcttca ggtgccgcac
     2941 caggccccgc ccactggaaa ggaacaacac caataggacg accggaatct acaccgctgt
     3001 cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg
     3061 ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc cacgataatt
     3121 tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg caaccggtgc
     3181 ctgatgttga ccgtccgaac agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt
     3241 ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca agtttagctg
     3301 ctgccaacgc agcctgacaa agtgcgggat cgccaccttc aggtttgata tccagtaaca
     3361 taccatcggg tgccagtttt atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt
     3421 catagaactt actttcgata gcagatttaa tctgcccggc atagttattg atatcggccc
     3481 ctgatgcgcc attgttttta gtattaccac tcccggcagg cgaagcattg ttccctttcg
     3541 cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa atatcatctg
     3601 cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgcc gctgccgctt
     3661 ttttgtctgc ggcggctttt tctgccgctg ctttcttatc agctgctgcc ttatcagcag
     3721 ccgctttttc tgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag
     3781 ctgcagcctt ttcagcagcc gctttcttct cagcttctgc cttggctttt tcagcagctt
     3841 cagttgccgc tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct
     3901 tcagtgccgc agcggctacc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt
     3961 ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt cgctgcttct tccgcagctt
     4021 tagcatctgc cgccgctttc gctgccgcca cttcagcttg cttctgcttt aactcggcct
     4081 gttttgcggc ttcttcagcc tgctttttct gctcctgagc cgctaaccgc tctttctcaa
     4141 gttgcttcag gcgttcctgt tcagccgctt gtttctcacg cagttcttca gcagcctgct
     4201 gttccttcat cttgcgctgt tcatcagaac gcttcgcgct tgattcctgg ctttgcatgc
     4261 gtttgtactg ctcaactacc gcacctgaat caaccatgac agcgtcgatg gacgaaccac
     4321 cgccgcctcc ggctgaagct tctatattct catcgaacga actccagatc agcgccgcaa
     4381 ataagatgac atgcagcact gctgaaataa ttatcgcccg cttgagcttg tcgttttgtt
     4441 cggttgcctt tgacactctc ggtttccaaa aactgttcgc ctgttacccg ctctctttca
     4501 agcaagggaa acgcagatgt ttagataggc tgcgtcatta aaccaaccga tttcacaccc
     4561 gcactatgta acaagttcag tgctttaatt atttcatcgt aaggcacatc ttttgcgcca
     4621 ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc tggacacttc cgccaccacc
     4681 tgctctggtg gtaaacgctc caggcgatct ttctcaacca ccacggtgta ctgaccaata
     4741 ccagacactt caacaatcac tggcggatta tcgttactgc tcaccgcctg tgattcagta
     4801 gcgtctggca gatcgacctc cacgctctgg gtgatgatgg gcgctgtcgc cataaagatc
     4861 agcaacagca ccagcagtac gtccagcaac ggaacaatgt tgatttcgga cttgagatcg
     4921 cgacgacctc gtccacgcgc tctggccatg gcttacccct tgttgctctc gctaacggta
     4981 aacgcctggc ggtgcagaat cgcggtaaac tcttccataa agttgtcgta attcagttcc
     5041 agtttgttta cgcgctggtt gagacggtta taagccatta ccgccggaat tgcggcgaac
     5101 agaccaattg ccgtcgcaat caacgcttct gcgataccgg gcgcaaccat ttgcagtgtt
     5161 gcttgtttta ccgccccgag ggcgataaag gcgtgcatga tcccccagac cgtaccaaac
     5221 agaccaatat acgggctgat ggagccaacc gtaccgagga acggaatgtg cgtttccaga
     5281 ttttcaagtt cacggttcat ggagatacgc atagcacgcg acgccccttc cactacggct
     5341 tccggcgcat ggctattggc acgatgcagg cgcacaaact ctttgaaccc gctgtaaaag
     5401 atttgttccg aaccagtcag attatcccgt ttcccctgac tctcttgata gaggcgagag
     5461 agttcgattc cagaccagaa tttatcttca aacgcttcgg cttcacgcgc cgctgcgtta
     5521 agaatacggg ttcgctggat aataatggcc caggatgcga ttgaaaaacc aatcaaaatc
     5581 aacatgataa gtttaaccag aaggctagcc ttcaggaaca aatcaaggat attcatgtca
     5641 gtcactgctt aaactccgcg acaatagact tgggaagcgc acgaggcttc attttgagtg
     5701 ggtcaacgca aacaaccaga acctctgctt cattcagcaa agtattctcg gcgttgacaa
     5761 tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt tatttcagtc tgtatttcga
     5821 gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt cattttacgt accacaaagg
     5881 caacgcgttc agccatcagc gcctgctgac tgaagtgatg atgacgcagc atctctgtgc
     5941 gtgctctttc ataaaaagcg acgtaactgg cgtggtacac cacaccaccg gcatcggtat
     6001 cttcatagta gacgcgaacc ggccatcgaa acagcgttgt attcacttta catcccggta
     6061 atgcaacaaa agttagagct tttaaacgct gctactatac gcgcgcgatt gaggtttggg
     6121 aatgagggca agttaagggc gcgtaaaaat ttatgggcct agagcggccc ataatttttt
     6181 aaagagaatt ctgatttaaa agaagaagaa aatcagcccg acaatgagaa caatatcggc
     6241 aagcaatggg caaaaaatcc cttgccaaag aaccttctgc ggacgaaagc ccacgccgtg
     6301 aatcacacca gcacagacgg cccacatcag caataaacca tgccagattt ccagttcact
     6361 ggtcttcgcg gcaaaacgag atgggtccca aaacatacat cctgctaaca gaagcgccat
     6421 cacgaaggaa agcgcccgca gggggcgctt gtccattacc gcatacaagg tcgcgataat
     6481 cttactcatc agatgtcttc ttgaccggct ttgcctgatt cgacgtgttc aagcgccagc
     6541 gcggtgatta ccccaaacga acaggcaaga agcgttccca gaatccatgc gaaataccac
     6601 atttttagct ccttacttag tacagagagt gggtgttacg ttcaatatct tctttggtga
     6661 tacgaccgaa cattttccag taacaccagg cggtgtagag cagaatgatc ggtaccagaa
     6721 ccaccgcaac ccaggtcatg acgttaagcg tcagctggct ggaagttgca tcccacattg
     6781 tcagacttgc gttcatcatg gtgctggacg gcatcacaaa cgggaacatt gcgataccgg
     6841 ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac aaacgcccac gcggctttat
     6901 ccatacgtgc agtcaggatg gtcagcagca gcagaaccac acccagtgcc ggaatagccc
     6961 acagaattgg cgtgttgttg aagttaacca gccatgcgcc agcttcacga accacttctt
     7021 tattcagtgg gttagaggct gcgtaatggt ccattgtcga tttcacgaca taaccatcga
     7081 taccgtacat cacccatacg ccagccagtg cgaaacagac cagtgtcacc agcgcagcca
     7141 cctgagccgt tgcacgggta cgcaggtgca gttcgcccac ggtacgcatt tgcagatagg
     7201 ttgcgccctg agtaatgatc atccctacgc tcaccacgcc tgccagcagg ccgaacgggt
     7261 taagcaactg gaagaagtta ccggtgtagt acagacgcag atattcatca acgttgaacg
     7321 gtacgccctg caacaggtta ccgaacgcta caccaattac cagcggcgga acgaagctac
     7381 caatgaagat gccccagtcc cacatgttac gccagcgggt ttcttcaatc ttggagcggt
     7441 agtcaaaacc gaccggacgg aagaacaaag acgccagcac gaggatcatc gccacataga
     7501 agccggagaa cgcagcggca tagaccatcg gccaggcagc aaagagtgcg ccgcccgcgg
     7561 tgatcagcca aacctggtta ccgtcccagt gtggtgcaat ggagttaatc ataattcgac
     7621 gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc cacccccatg tcgaaaccgt
     7681 cagtgactgc aaaaccaatc agcagaacgc caaccagcag ccaccagata aaacgcaata
     7741 cttcataatc gatcatttga cgactcctgt cttagcgtgc cggctgagta gtcgtggaag
     7801 actgctcaaa gtgatagcga ccggttttca ggctgcttgg gccgaggcgt gcaaacttga
     7861 acattaagaa caattctgcc accaggaaca gggtatacag gccgcaaatc agcaccattg
     7921 agaagatgag atcgcctgcg gtcagtgacg aattcgccac agctgtcggc agcacttcac
     7981 cgatagccca cggttggcgg ccatattcag ccacgaacca gcccgcttct acagcaatcc
     8041 acggcagcgg aataccgtac agcgcggcgc gcagaagcca ttttttctcg ccaatgcggt
     8101 tgcggatgac actccagaaa gagagcgcga tgattgccag cagcaggaag ccacacgcca
     8161 ccatgatacg gaacgcaaag tacagcggcg ctacacgcgg gatggagtct ttggttgcct
     8221 gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt atagcgtttc agcagcagac
     8281 cgtaaccgag gtctttcttc atgctattga actggtcacg aaccgtctgg tcggtagaac
     8341 cagaacgcag ttgttcgagc agagagtacg ccttcatccc gttacgaatg cgttcttcat
     8401 gctgcaccat cagctctttc aggccgataa ccggggtatc cacggaacgc gttgcaatga
     8461 tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt ctcctcttcc tgatcaggaa
     8521 tgccgaacaa agtaaaggca gcaggcgcag gttgcgtttc ccactcggct tcaatagcag
     8581 ccagtttggt tttctgcacg tcgcccattt cgtagccgga ttcatcaccc agaacaataa
     8641 cagacagaac agcagccata ccgaagctgg cagcgatagc aaaggagcgt ttagcgaagg
     8701 cgaagtcacg acctttcagc atataccatg cgctgatacc gaggatgaac atcgcgccag
     8761 tcacataacc agacgctaca gtgtgaacga atttcacctg agcaaccggg ttaagcacca
     8821 gctcggagaa gctcaccatc tccatacgca tagtttcaaa gttgaaatcg gacgcgattg
     8881 ggttttgcat ccagccgttc gcaaccagaa tccacagtgc ggacaggttt gaaccgagcg
     8941 ccaccagcca ggtgacacac atatgctgaa ctttacccag acgatcccaa ccgaagaaga
     9001 acagacctac aaaggtggat tcgaggaaga aggccatcag accttcgatt gccagcggcg
     9061 caccgaagat atcccctaca tagtgggaat agtaagacca gttagtcccg aactggaact
     9121 ccatggtcag accggtagcc acacccagag cgaagttgat accaaacaac ttgccccaga
     9181 acttggtcat atctttataa atctgtttgc cggagaggac gtagaccgtt tccataatgg
     9241 ccaggaggaa cgccataccg agcgtcagtg gcacaaaaag gaagtggtac atcgcggtca
     9301 aggcaaactg taagcgcgac agttcgacta tatctaacat catgactcct tgctcatcgc
     9361 atgaagactc cgagagtgaa ccccgttaga aagggtcaca cgcatgcccc aatagaaatt
     9421 tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa aacggcgtaa agaaaggttg
     9481 caaacatgtt aataaaaact caaattgatc ccacgtatat attacgccgc aaaatcctta
     9541 caataaacag gtttttgttg aagcaaattt gcatttttcg acggtgatca atttatagct
     9601 aaattgccgc ctttcagcca atttgatcga gaacaattta tctctttttg atgcccattt
     9661 ccaagactta tacattgata aatatcaatt tctacaaatt atgttaatca tgtttcaaca
     9721 aggtaacttt agtgatattt atgtttaaaa tgtgtgaatt ataagactga aattagtttc
     9781 ggttggcaat taaagataaa aagagacgaa gcaaaaacag cacggttgac cttaattctt
     9841 tttttggtca ctggctaata cgacgcagag tttcaggatt attaataaag gcataaatct
     9901 cccccccgcc gatattaaca tttaattaac ttataattat attataatta actcacccgt
     9961 ggttattatt ttgttaaaaa taaagccaca tatacgtagc ttgagagtga agataaacac
    10021 aaaattacct gatgcgctac gcttgccagt gtctacatca tctctgcaat atattgaatt
    10081 tgcctgcttt tgtagaccga ataaagcgtt catgccgtat ccggcatgaa caaagcgccc
    10141 tttgtcaaca atctggccgc gcatatgtgg cttcggtttg attgctattc aggcaagccg
    10201 gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc gatagatttg aaccttgatt
    10261 ttcctcggtg gtaatgtgtt catccatcat cgtttctgag caagaaatca cctcgcgact
    10321 gaaagcaaca gtcgcatcac aggttgctga ttcagccgga ttaaacagcc gtaaaatcac
    10381 ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg catcccactg ggggcatttt
    10441 caacaaacta taactttccg gcacgttgaa tccggctttg ttgagcttca tcgcatccca
    10501 tgggattttg ttgtaacact gtactggagt cagccatgct cgcgcctgct gagctacacc
    10561 agcggcggtt ggcgtaccgg tataactcaa taaacttagg cgacaagaaa gcagaccacg
    10621 tagttgtgag tccgggactg gcattttaat tcccgaaggc cgcctaggcc ttaaaagcag
    10681 atcttctttc cccagtaagc ccacgccgcg cagcaacgta atggcaaagg ttttcttctc
    10741 ttcaccgatg acttcaaatt cacgtagccc ttcgctaaag acagccatgc cgttacgccc
    10801 ttcctgtaag gctgcatagt tgagcatatt ccataccgga accggcgctt ctttccagct
    10861 ttcttgctgc cagttgttca ttgcactgtc gttcacgggg cgcgttagcg aaccaaactg
    10921 ggtatctgcc agaacactat cggtgttaaa agatgtaggg atcaggacgc gaaggcgatg
    10981 atcgtcagcc tggttatcaa ggttgatatc cacatcaatg cgcctgttgt tatgactaag
    11041 agtgacaacc aactcgacat ctactctgcc agtggattgt cttgcgctgc gttgttgcag
    11101 attacgcggc accgccattt catagcggat aacagccctg ctctgccagg cctcatgaat
    11161 aatctcgcat tgcggtttcg cattcgccga agtaattacc cactcttctt ttgcgggtga
    11221 atagtcatat tcatcaccat catcagagct ttcttcaatt tgcaacaccc ggtcatagcg
    11281 cacaccgcta tctttatcta ccagttgtag cgttccatcc tcattgagcg caatttgcca
    11341 gaaagcattt tccagtatcc cttcagcgtc actttttgcc gcaatcacgt tgccaggctg
    11401 attcgcttcg atataaagcg tgcgatagcc catagaaggg acaatctggt tgatctgtat
    11461 atcaaactcc ataaagggat cgtaattacc gtaatgaact atttgccgat cgattaggcc
    11521 tggatcgatc tcacgggcat ggcgaataaa atacggtaca ggctgaccgc gatcgtctcg
    11581 tagattaaac tggctggcgc gcagccgcac agtggtgttg atcacttctt cccgcggcca
    11641 gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc tgcggcatgt tgtcggcgat
    11701 tttacgcata tagaaacgca gcagattatc cgccatgtct tcagccagtt cgaagcgggc
    11761 gacgatttcg cgatgaactt tgtcacgaca gcagcagccg atgctgtcgt gggcatgatt
    11821 ttttaagatc tctttccaca ttttctccag caagccgtgg tggtatccaa aacccaacgt
    11881 cctggccagc gttgccagcg gttccagcag attaacaatc ttattttcaa tacgtgcgtg
    11941 ggcaactttg atatccatgc gcgtggaacc gatggtgcga tgcacgcgca tatatttgcc
    12001 atcaataaat tcccctttca gggttgccag attatctcgc tgcgcttcga tcttctcaaa
    12061 tacctcttca aagcggctca tcacaaactt tcgttgagga taaatttcgc gcagtttctc
    12121 catcacttcg aagatatttt gctgcaatgg catctgatca tgcccattcg gcagcaaaat
    12181 ctctttggtt acagacgctt tttccagcac gtcaaaataa ctgtcgaggc gtttgcgtaa
    12241 tccgttttcg tccgcaggta agtacttacc gatggcataa cccaacggca tcacctgcgc
    12301 cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg gttttatccg taccgtggcg
    12361 ctcagaacat ccacgccaga acatggtgca ggtaatgcca aatccattgt agatatgcgg
    12421 cagttgcccg gacatgccaa aggaatcggg taaataacct attttcatcg gctcgccaaa
    12481 cgcgaggcag tcacgcattc cgtacatcag attacggacg atggattccg cagaaacaat
    12541 cgtggtatcg gtctgggtat accaggggcc gataatcaac ttgccggctt ctaccagttt
    12601 cttcaagcgg tctttgtttt ccggtttcac cgcgaaataa tcttcgagga tcgccgtttg
    12661 cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc agtcggcaca ggatctcttc
    12721 catattattg accagcagaa tgcgtgactc ttcggtggtg aaataccact cccgatccca
    12781 gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc atcgtcgttt cctttttgtt
    12841 tatggcatta cgccatcagt cagatagttg ccatgcttaa ccgcgtgacg ccgccagatc
    12901 aggagaattg cagtcgagat tgcagccccc accaatgccg caccaaacca gccaattgcc
    12961 gccataacac cgcccgcgcc attatcatga agtaaaaaga gcgagaaaat gccagcaccg
    13021 ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc ccgttaccat agagcccagc
    13081 acaaacgaac caataacccg cagcggatct tcaatcgcca tcgggatcgc cccttcggta
    13141 atacctgcca gccctaacag ccaggtggat ttcccggtct caatttcaaa ctctttaaac
    13201 aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg ccgaaaccat tttgacggag
    13261 gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg cgcccaggca gaatgcataa
    13321 gcggctttat tcactggccc tccaaggtca aaggaacaca taaaaccgag aatggcaccc
    13381 agcaacagcg cgttacttcc tgacagaccg ttcagccagg cggtaagcga gttattgatc
    13441 caggcgacag gttcccccac cacaaacagc atcagactgc ccgctcccaa tgtaccgagc
    13501 accgggtaga gataaaaagt caggaagcca ttgaatttac tgctaagacg caagtgattt
    13561 ttcatccagc gtatcaagta accggctatc aatccgccaa cgaccgcgcc gagaaacccg
    13621 gagccgatca tgttggcggc aagtccagcc gcaaagcctg gcgctaacgc cggtttatct
    13681 gccagcgaat aggcggtata ggctgccagc accggtacca tcaaaattcc gagcagcccg
    13741 ccgcccagtt tgcggtacat ccacagccag gagttttctt cattaaacag atcttgtagc
    13801 ccgaagattt gcgacagtaa taccgcgacc gccagcaccg tcccccccgc gacaatcaac
    13861 ggtacggcaa aagagatccc gctcaacagt gcctgtttca gctccgtttt gacgcttttc
    13921 accggttgcg tatcttgctg taaggtacgc gtctcatcgc cacgctcgag ggtaagcgat
    13981 tgttggatca acgcttctgc atggcgaatc ggctcggcaa caggcacaga aagcgcagga
    14041 atgccgttaa aacgctcact ctccttgatg gcgacttcag ccgcaaaaat acaggcagtt
    14101 gcactattga gttgatccgc cgttaaacgc ccttcaatac cgttagcacc ttgcttttca
    14161 acgtagacat tcacgccgag tttgcgtccg gctttttcca gatactccgc agccatgtag
    14221 gtgtgagcaa tacccgccgg gcaggccgtt acgcaaacga tagttggcgc gttggaaaaa
    14281 gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg agagcaattc atcaggcgtc
    14341 gtcgccgact gtatacgcgc ccgaatctca tcatccgcca ggcgcgtggt cagcgctgtc
    14401 agcagttgca tgtgcgtagt acccgcttca ttgaggggaa tcgccagcaa caccactaaa
    14461 tcaaccgctt ccgggccatc cacgccttcc cactgaagcg gctcgctgag tgtggcgacc
    14521 gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg gcacagccaa cccttcactt
    14581 aaggcggtcg ggccaaggct ttcacggcga tacacttctt tcagaaattg ctcagtactg
    14641 gaaattttcc ccagagcagc aagacgttga gtcaacgcgt gaatggcctc ttcacggctg
    14701 gtaaagcgcg cattcagaca caacgcatcg cggtgggtca gagtcgtcag gttcatcatg
    14761 atcctcactt gatcgttttt atagtcgcgc cagtgtgccc gacaaaacaa taccattgcg
    14821 taatcaattt cacttgattg aaattatttg cattttattt gtattgttta caggatgagt
    14881 ttatcgggca taatggctaa attcgtatta atgagatgct aaataatggg acacaaaccc
    14941 ttataccgac agattgctga tcgcattcgt gagcaaattg cgcgtggcga gcttaaacct
    15001 ggcgatgcgt tacctaccga atcggcatta caaacagagt ttggcgtcag ccgggttacg
    15061 gtgcgtcagg ctttgcgcca gttagtcgag caacagatcc tcgaaagcat tcagggcagc
    15121 gggacttacg tcaaagaaga gcgggtcaat tacgatattt ttcagttaac cagttttgat
    15181 gaaaaactgt cggaccgtca cgtcgataca cacagtgaag ttctgatatt tgaagtgatt
    15241 ccggctgacg attttcttca gcaacagcta caaatcaccg tgcaggatcg tgtttggcat
    15301 gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc tggaagaaac ctggatgccg
    15361 cttgcgttgt tcccggatct cacctggcag gtcatggaaa attcgaaata tcactttatt
    15421 gaagaagtga agaagatggt tatcgatcgt agcgaacagg aaatcattcc tctgatgcca
    15481 accgaagaga tgagccgtct gcttaatatc agccagacaa agccgattct tgaaaaagta
    15541 tcgcgtggat atttagttga tggtcgggtg tttgaatata gccgcaacgc ttttaatacc
    15601 gatgactata aatttaccct gatagcgcag cgaaaatcat cgcgataagc acaaaaaggc
    15661 catcatacga tggccaacca tgtcgaaacc gacagccctt cgccattcct gtagagggaa
    15721 gcggcgaggg ctatttctta ttacagatat ttatttcaga acagttttca gtgcttcacc
    15781 gatatccgcc aggctgcgaa cggttttcac gcctgcggct tccagagcag cgaatttctc
    15841 atccgcagtc cctttcccac cggcaatgat cgcacccgcg tggcccatac gtttgccttt
    15901 cggcgcagtc acaccagcga tgtaacccac aactggcttg gtaacatgct ctttgatgta
    15961 cgctgccgct tcttcttcag cgctaccgcc gatctcaccg atcatcacga tcgcttcggt
    16021 ctgcggatct ttttcgaaca tttcgagaat gtcgataaag ttagagccag ggatcgggtc
    16081 accgccgata ccgacacagg tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc
    16141 ttcataggtc agtgtaccgg aacgggaaac gatacccact ttacccggtt tgtgaatgtg
    16201 acccggctgg ataccaattt tgcattcacc cggggtgata acgcctgggc agttcgggcc
    16261 gatcatacga acgcctgctt cgtccagttt cactttcacg gtcagcatat ccagcgtcgg
    16321 gatgccttca gtgatggtga taatcagttt gatgcctgca tcgatagctt ccagaatgga
    16381 gtctttgcag aacggtgctg gtacgtaaat aacagacgcg gaagcgccag tagcagcaac
    16441 agcttcacgc acggtgttga acaccggcag gccgaggtgg gtagtgccgc ctttacctgg
    16501 agttacgcca ccaaccattt tagtgccgta tgcaatggcc tgttctgagt ggaaagtccc
    16561 ctggctaccg gtaaagccct ggcagataac cttggtgttt ttatcaatta aaatggacat
    16621 tatttcccct ccactgcggc aacaacctgc tgagctgcat ccgtcagacc ttttgctgca
    16681 ataatattca ggccgctgtc agccagtttc ttcgcgccga gttcggcgtt gttaccttcc
    16741 agacgtacca cgaccggtac gttaacaccc acttccgcta ccgcgccgat gataccgtca
    16801 gcgatcaggt cgcaacgaac gataccgccg aagatgttaa ccagaacggc tttcactttg
    16861 tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt ctttggttgc gccgccgcca
    16921 acgtccagga agttagccgg ttcgccgccg tgcagtttaa ctatgtccat cgtacccatc
    16981 gccagacctg cgccgttaac catacaaccg atgttaccgt ccagcgcaac gtagttcagt
    17041 tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg catttcgcgc
    17101 agatcaggct ggcggaacag ggcgttgcca tcagcgccca gtttgccgtc gaggcaaatc
    17161 agatcgccct gtttggtgat gaccagcggg ttgatttcga tcagcgccag gtcgcgctcc
    17221 aggaaaatgg tcgccaggcc catgaagatt ttggtgaact gctgaaccag tttaccttcc
    17281 agacccagtt tgaacgccag ctcgcgtccc tgatacggca tcgggccagt cagcggatca
    17341 agcgcaactt tatggatcag gtgcggagtt tcttccgcca ctttttcgat ttccacgccg
    17401 ccttcggtgg aggccataaa gaccacgcga cgggaactac ggtctacaac ggcaccgaga
    17461 tacagctctt tagcgatatc ggtcgccgct tcaaccagaa tctggttaac cggttggcca
    17521 ttggcatctg tttgatacgt taccagacgc ttgcccagcc agttttctgc aaaagcacgg
    17581 atgtcttctt tgctgtttac aactttcaca ccgcccgctt taccgcggcc accagcgtga
    17641 acctgacatt tcactaccca cggaccggca ccgatttttg aagcggcttc ttctgcttcg
    17701 cgcggagtag tacaggcata acccaccggt gctggtaagc catagcgggc aaacagttgt
    17761 tttgcctgat attcatgtaa gttcatgtgt tctgtccatc cttcagtaat cgttatcttt
    17821 taaaccgtag accgacaccc tatccacgtc ggtctacagt gcaggtgaag cttaaactac
    17881 tacacgtcca gcagcagacg cgtcggctct tccagtaact ctttgatcgt taccaggaag
    17941 cccacggatt cgcgaccatc gatcagacgg tgatcgtagg acagcgccag gtacatcatc
    18001 ggcaggatct caacctgacc attcaccgcc atcggacgat ctttgatagc gtgcataccc
    18061 agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca tcagggaacc gaacacacca
    18121 ccgttggtga tggtgaagtt accaccggtc agatcttcaa ccgtcagctt gccatcacgg
    18181 cctttgactg ccagctcttt gattttcttc tcgatgtctg ccatgccgag ggtatcgaca
    18241 tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa ccgccatgct gacgtcgaaa
    18301 tagttgtggt aaaccacgtc atcgccgtcg atagacgcgt tcacttccgg gtaacgtttc
    18361 agggcttcaa ccaccgcttt cacgtagaag gacataaagc ccagacggat gccgtggcgt
    18421 ttttcaaacg cttcaccgta ctgcttacgc agatccataa tcggcttcat gttgacttcg
    18481 ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca gcagacgctc tgccacacgc
    18541 ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg cagccagagc cggttgcgcc
    18601 gccggagcag ccgctgccgg agcagactct ttcgccgggg ctttcgccag atgtttttcc
    18661 acatcttcac gagtcagacg accacccaca ccggtgcctt taatggcgct ggcgtcgaga
    18721 ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg catcgttgtt ttgctcttcc
    18781 agagacgcct gctggcgttg cgccggagtg gacgctttct cttcagattt ggcgctggtt
    18841 tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga cgttaccgtt
    18901 gtaccttcat cttccagaac cgcatccaga atgccgtctg ctgatgccgg tacttccagt
    18961 accactttgt cagtttcgat ttctaccagc acttcatcac gtacgactgc gtcgccgggt
    19021 tttttatgcc aggttgcgac ggtggcatcg gctacggatt caggcaggtc agggaccaga
    19081 atatctacgc tactcattgt gtatccttta tttattcgac gttcagcgcg tcattaacca
    19141 gatcttgttg ctgtttctgg tgaacggaca tataccctac cgccggagag gcggaggccg
    19201 ggcggcctgc ataacgcaga gaagccccaa acggaatcac ttcacggaaa tgatgctggc
    19261 tgcagtacca tgcgccctgg ttgagcggct cttcctggca ccagacaaaa tccttgacgt
    19321 gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag agttgctcga
    19381 tacgcacaat ggcgacatcg tgttgattgt tcttacgacg ctgttccagc aggtcgtaat
    19441 aaaccttacc agaacacatc actacgcgct tcacgccctt cggatcaagc tcgtcgattt
    19501 caccgatggc tggcaggaag gtgccgttcg ccagttcttc gaggctggaa accgccagcg
    19561 gatgacgcag cagggatttc ggcgacatca cgaccagcgg acgacgcatc ccgcgcagcg
    19621 cctgacgacg cagcatgtgg taaacctgtg ccggggtaga cggtacgcaa acctgcatgt
    19681 tttgctcagc acaaagttgc agataacgtt ccagacgcgc ggaggagtgc tccggcccct
    19741 gcccttcgta accgtgcggc agcaacatca ccagaccaca catccggccc catttctgct
    19801 cgccagagga gatgaactgg tcgataacca cctgcgcacc gttggcgaag tcaccgaact
    19861 gcgcttccca gatggtcagg gtgcgtggtt ctgcggtggc ataaccatat tcaaacgcca
    19921 gcacggcttc ttctgacagt acggagtccc agacacggaa cgcgccctga ccgttatgga
    19981 tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc gcgtggcggt
    20041 ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg aaccggaatg ccttcatcaa
    20101 ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc gaacagtttc tcaccggctg
    20161 ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga ctgcatttca actgcttctg
    20221 gtaccgtact gatacgttta gccagttcct gcaggcgctt catctcaact ttgttcgggt
    20281 actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg
    20341 gacgccactc tgccactacg caatcgccag catccagcgc atcgcggtac aggttaacca
    20401 tctcggtggc atcttccagc gtcgccactt tttcctgctc cagcttgtca gcgtagattt
    20461 tgcgcggtgt cggatgtttt ttgattttct gatacatcag tggctgggtt gcgctcggct
    20521 cgtcggcttc gttgtggccg tgacggcggt agcataccag gtcgatgaag acatcacgtt
    20581 taaaggtgtt acggaaatcg agcgccagac gggtcacaaa ggcaacggct tccggatcat
    20641 ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc gatatcagta cagtacggtg
    20701 tagaacgggc atccagcggg ttagaggtgg tgaaaccaac ctggttgttg ataacgatac
    20761 gtaccgtacc gccaacttca taaccacgcg ctttcgacat gttcagagtt tcctgaacca
    20821 cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat tggcagcact ttgttgctgt
    20881 tcggctcatc aagtctgtcc agacgggcac gaacagagcc gataacaacc gggcttacaa
    20941 tctcaaggtg agacgggtta aacgccagcg ccaggtgcac caggccgcca tcggtctgga
    21001 agtcagacga gaagcccatg tggtatttca cgtcgcctgt gccgaggtgt tctttatgtt
    21061 taccggcgaa ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc accagcacgt
    21121 tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg ggtgccgctg ttgccagcgt
    21181 ggcggatcat ctctttaagc atcgggatta acgcgtcacc gccttccagc gagaagcgtt
    21241 ttgcgccagg gaattttgcg ccgaggtaac gctcaaggcc ttcagcggcg gtcagttcgc
    21301 ttaagaagcg ttttttctct tcgctattga aagtcgcgcg accagactcg atacgctgtt
    21361 ggatccagcg tttttcttcg gtgctggtga tgtgcatata ctcggcacca atcgggccgc
    21421 agtaggtttg cttgagggcg gaaataagct cgccgagttt catggtttct ttgccgctgg
    21481 caaatgaacc gacgttgaag gtttcctgga agtctgcttc ggtcagatcg tggaaagacg
    21541 gatccagatc ggccacttta tcttgctgcc acagtcccag cggatctaga ttcgcatgct
    21601 ggtgaccacg gaagcggtat gcgttaatga gctgcaggac tttaacctgc ttcacattgg
    21661 tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat
    21721 attcacgcgt ttgagagtgg aattgatccg gtttgactcc cgtaccaggt aactgctgga
    21781 acgtcgaacg ccagttagcg tcaaccgagt caggatcggt taagaagtct tcatagagct
    21841 gttctatcca gctctggttt gcgccagaga ggtaagaaga gtccaaccag gctttcaaag
    21901 cgctgttctg catcgtgatc ccttaagcat cttttttatg cttacttcgc cgtggatact
    21961 accacgcaca gtgatgtgcg tgccggggtt cacttgcgag ctcttgtcta cgtagtggct
    22021 cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca gtttttaaag atttcctgca
    22081 tttctttgtt acgcctgatg cgctgcgctt atcaggcctg cgggtaatga cttgtaggcc
    22141 ggataaggcg ttcacgccgc atccggcact ggttgcctga tgcaacgctt gcgcgtctta
    22201 tcaggcctac agtttacgca ttacgttgca acaacatcga cttgatatgg ccgatggcgc
    22261 gcgtcgggtt cagccccttc ggacatacac tgacgcagtt catgatgctg tgacagcgga
    22321 atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta tcacggctat
    22381 cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc gataaactta tcgggattcc
    22441 accagaaaga cggacaagag gttgaacaac atgcgcagag aatacattca tacagcccgt
    22501 cgagtttttc gcgctgctct ggcatctgta aatgctcgcg agctggcgga ttttgtccat
    22561 tattcaacag gtaaggctta attttctcat attgcgcata gaattgtccc atgtctacca
    22621 ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat cacaatcttc ttgcccggct
    22681 ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt cttgccgttc atgttcagac
    22741 cgtcggaacc gcacacacct tcacggcagg agcggcggaa cgacaggctg ggatctttct
    22801 ctttcagctg gataagcgca tccagcagca tcatgtcgcg accttcttcc gcttccaggg
    22861 tgtaatcctg catacgcgga gcatcatcaa catccgggtt atagcgataa attgaaaact
    22921 cgagtctcat tttcctgtct ccgcattagt aagtacgaat cttcggcggg aatgccgggc
    22981 gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga ttccgactca ggcagataca
    23041 gggagtggca cagccagttt tcatcatcac gatccgggaa gtcgaagcgg ctatgtgcgc
    23101 cacggctttc ggtacggaag ttggcagaaa cagccgttgc atacgccgtt tccatcaggt
    23161 tatccagttc caggcactca acgcgctggg tgttgaactc gctggaagtg tcatccagac
    23221 gggcattttt cagacgctcg cggatcactt tcaactgctc aagccctttc gccatcgcat
    23281 caccttcacg gaagaccgag aagttatgct gcatacattc ttgcagcgct ttacggatcg
    23341 ccaccggatc ttcaccgtta cgattattgt tccaccggtt caggcgatcc agagacgctt
    23401 caacatcaga ctcgctggca tcgtgcagtg cgccctgctc ggcgatagac tcttgcagat
    23461 gcagacctgc cgcgcgacca aagaccacca ggtccagcag cgagttgccg cccagacggt
    23521 tagcgccgtg taccgataca caagcgattt caccaacggc aaacagtccc ggaacaacca
    23581 catcttcgcc tttctcattc acagtcagcg cctgaccggt aactttggtc ggaataccgc
    23641 ccatcatgta gtgacaggtt gggataaccg gaatcggctc tttcaccgga tcgacgtgag
    23701 cgaaggtacg ggaaagctcc aggatacccg gcagacggga ttcgagaact tctttaccca
    23761 ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc atcacagccg cgaccttcac
    23821 ggatttcaat catgatggaa cgcgccacca catcacggcc cgccaggtct ttggcgttcg
    23881 gcgcataacg ctccataaag cgttcgccat gtttgttcag cagataaccg ccttcaccac
    23941 ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc ggtcgggtgg aattgccaca
    24001 tttccatatc ctgcaccggt acgcctgcgc ggatagccat gccgacaccg tcgccagtgt
    24061 taatgtgggc gttagtggtg gactgataaa tacgccctgc tccgccagtc gccagcacgg
    24121 tagcgcgggc tttgaaataa accacttcac cggtttcgat gcacagtgcg gtacaaccca
    24181 ccaccgcgcc atcctggttt ttcaccagat ccagcgcata ccactcggag aaaatggtgg
    24241 tgtggttttt caggttctgc tgataaagcg tgtgcaacag tgcgtgaccg gtacggtcag
    24301 cagccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga ccgccaaacg
    24361 gacgttgata gatacgacca tcatcgagac gcgagaacgg caggcccatg tgttcgagtt
    24421 ccagaatcgc ttccggcccg gttttacaca tatattcaat cgcgtcctgg tcaccgatat
    24481 agtccgaccc ttttacggtg tcgtacatat gccattccca gttatcttca tgggtattac
    24541 ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc gggaagactt
    24601 tagagagcag ggcacaggtc tggccgctct gggaaatttg cagcgccgcg cgcatacctg
    24661 cgccaccggc accaatcaca actgcatcaa attctctgac tggcaatttc atcacacacc
    24721 ccacaccaca acgaatccat aaatcacgta aaccaccagt gcaacgacaa tcaccagttg
    24781 cagcatcagg cgcaaggcca gcggtttaac gtagtcggtc aacacctgcc acatgccgat
    24841 ccaggcatga atcaagatag aaaacagcgc cagcagggtg aacactttgg tgaacgcaga
    24901 ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca ctggtggcga aaaaaccgac
    24961 catataaatg atgtagagcg tcaggacgat agcggtagcg cgaacgagga tgaaatcatg
    25021 tacgccattg cgtcctaatg cggaggcgtt gcttaccata cgaggactcc tgcgagaagt
    25081 gaaagcacga cagtaataac aaaggagatt ttggcggagc gtttacccgc ttcgaatgtt
    25141 tcttccagat agccaaaatc catcatcatg tggcgaatac ctacgacgac gtgatacgcc
    25201 agagcggtaa ggatgcccca catgataaat ttgacgaaga agctgcccat aatcgcggaa
    25261 gcttgctcga aaccttcagg ggaagagagg ctggtaccca gaagccactg caggatgccc
    25321 actgcaacaa aggtgatcac accggaaacg cgatggagaa tggacgctat cgccgtgatg
    25381 gggaaccgga tggtctgtag gtccagatta acaggtcttt gttttttcac atttcttatc
    25441 atgaataacg cccacatgct gttcttatta ttccctgggg actacgggca cagaggttaa
    25501 ctttctgtta cctggagacg tcgggatttc cttcctccgg tctgcttgcg ggtcagacag
    25561 cgtcctttcc ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa aacgacacgt
    25621 tacaacgctg ggtggctcgg gattgcaggg tgttccggag acctggcggc agtataggct
    25681 gttcacaaaa tcattacaat taacctacat atagtttgtc gggttttatc ctgaacagtg
    25741 atccaggtca cgataacaac atttatttaa tttttaatca tctaatttga caatcattca
    25801 acaaagttgt tacaaacatt accaggaaaa gcatataatg cgtaaaagtt atgaagtcgg
    25861 tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac
    25921 agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta
    25981 ttcgtcatca atggattctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc
    26041 tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc
    26101 aaatttaagt tccggcagtc ttacgcaata aggcgctaag gagaccttaa atggctgata
    26161 caaaagcaaa actcaacctc aacggggaca cagctgttga actggatgtg ctgaaaggca
    26221 cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg
    26281 acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg
    26341 aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc
    26401 tagaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat
    26461 ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccacg
    26521 ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg
    26581 cgttctatca cgactcgctg gatgttaaca atcctcgtca tcgtgaaatt gccgcgttcc
    26641 gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc
    26701 catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct
    26761 ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc
    26821 tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct
    26881 cttcgggggc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg
    26941 cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac
    27001 acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct
    27061 tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc
    27121 atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg
    27181 aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt
    27241 tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt
    27301 tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac agttacggta
    27361 tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg
    27421 atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg
    27481 cgggttaatt tttatatgcc taaacacaaa aaattgtaaa aataaaatcc actatcggac
    27541 attcatatat tctaaaaaaa aatacatcgg tataaattat aaacaaatag atgattttta
    27601 atcatctatc ttcctctcag ttacatgaat aaaccaccaa atatagcgca cccctataat
    27661 tcccaatact ttattcatca aacatttaat tcataccatc aattaattac ttttacattt
    27721 aataaaatta ataatatttt taagaaaaac taaaaacatt cgacaatcaa ataagatatt
    27781 cttgcaccga tatacttttt tcttttctgt taaaggactc agttatgttt aaaggacaaa
    27841 aaacattggc cgcactggcc gtatctctgc tgttcacagc acctgtttat gcggctgatg
    27901 aaggatccgg tgaaattcac tttaaaggtg aagttattga agcaccgtgt gaaatacatc
    27961 aggatgatat tgataaagag gttgaactcg gtcaggtgac caccagccac attaatcagt
    28021 cacatcacag cgatgccgtt gctgtcgacc tgctcttagt caactgtgat ctggaaaact
    28081 ccagcaacgg ttccggtggc aagatttcta aagttgcagt cacctttgat agctcagcga
    28141 aaaccaccgg cgcagatccg attctcaaca acaccagcac gggtgaagcc accggcgtcg
    28201 gcgtacgttt aatgaataaa gatcaaagca atatcgtttt aggcaccgct actccagata
    28261 tcgacctggc tccgacctcc agcgaacaaa cgctgaattt ctttgcctgg atggaacaaa
    28321 ttgatcaggc tacacctgta acgccaggcg cagttacggc gaatgcaacc tacgtgctgg
    28381 attataaata agtattactt tggcggcgct atcgccgcct ttcataaaat atttatcagg
    28441 tatggacacc gtgaatattt atcgactctc ttttgtatcc tgcctggtcg tggcgatgcc
    28501 ttgcgcattg gcggtcgaat tcaatctgaa tgttctcgat aaatcaatgc gcgaccgcat
    28561 tgatatttca ttattaaagg aaaaaggagt cattgctccc ggtgagtatt ttgttagcgt
    28621 tgcggtaaat aataaccaaa tcagtaacga gcaaaagatt aactggcaca aaaatgacga
    28681 taaaaccatt ccgtgcatca atgatttact ggtcgataaa tttggcttaa aacctgaagt
    28741 ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt agctcccgac ctgaaatgct
    28801 cttcaatttc gatcaagcca atcagcaact gaatattagt attccgcaag cctggctggc
    28861 gtggcactca gaaaactgga ccccaccctc cacatggaaa gaaggtgtcg ccggtgtcct
    28921 gatggattac aacttgtttg ccagcagcta ccgcccacag gatggcagca gcagcactaa
    28981 tcttaacgcc tatggaacag ccggaatcaa cacgggggca tggcgcttac gcagtgatta
    29041 tcagttgaat catactgata gcgatgataa ccatgaacag tcaggcgaaa tatcacgtac
    29101 ctatcttttt cgcccgttac cgcaattagg ttcaaaatta accctcggcg aaacggattt
    29161 tagtcccaat attttcgacg gtttttctta taccggcgcg gcactggcaa gtgacgatcg
    29221 aatgttgcca tgggaactac gcggctacgc cccacaaatt agcggtattg cacagaccaa
    29281 tgccacggtg acgatcagtc aatcaggccg cgtcatttac cagaaaaaag tcccaccagg
    29341 cccatttatc attgacgacc ttaatcagtc tgttcagggc acactggatg tcaaagtgac
    29401 ggaagaagat ggtcgggtga acaatttcca ggtttcagca gcatcgacgc ccttcctgac
    29461 tcgtcaggga caggttcgct ataaactggc cgcgggtcag ccacggccct ccatgtcaca
    29521 tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc tgggggatgc tgtcaaacac
    29581 ctcgctgtac agcggcctgc tgctttctgg tgatgactac cattctgccg caatgggtat
    29641 tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat gtcacgtggg ccagtagcca
    29701 ttttgatact cagcaggacg agcggggctt aagctaccgt tttaattaca gcaaacaagt
    29761 ggatgccact aacagcacga tttccctcgc cgcttatcgt ttctccgatc gtcattttca
    29821 cagctacgcc aactatctgg atcacaaata caacgacagc gatgcgcagg acgaaaaaca
    29881 gacgatcagc ttatctgtgg gccaaccgat taccccacta aacctcaatc tttacgccaa
    29941 cctgctacat caaacctggt ggaatgcaga cgcctccacg accgccaaca tcacggcagg
    30001 ttttaatgtt gatattggtg actggagaga tatctctatt tctacgtcat tcaatacaac
    30061 ccattacgaa gataaagatc gcgacaacca gatttacctg tcgatttcgc tccccttcgg
    30121 taacggtggt cgggttggct atgacatgca aaacagtagc cacagcacca cacaccgcat
    30181 gtcgtggaac gatacgctgg atgaacgtaa tagctggggc atgtctgccg gactgcaatc
    30241 cgaccgtcct gacaatggag cccaggtgag cggtaactat cagcacctga gttcagcggg
    30301 tgagtgggat atttctggta cctatgccgc caatgattac agttccgtca gcagcagctg
    30361 gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt catcgccgca gctccaccaa
    30421 tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat attccggttc agggcaatct
    30481 cgactacacc aaccattttg gcattgcggt ggtgccgttg atttccagtt accagccttc
    30541 caccgtggcg gtgaacatga atgacttacc cgacggcgta acagttgcag aaaacgttat
    30601 caaagaaacg tggattgaag gcgcgatagg ttacaaatca ctggcttccc gttccggtaa
    30661 agacgttaac gtcatcattc gcaacgccag cggtcagttt cctcccctcg gagcggatat
    30721 ccgccaggat gacagcggca ttagcgtggg gatggttggc gaggaaggac atgcctggtt
    30781 aagcggagtc gctgaaaatc aaaagtttac cgtggtctgg ggtgatagcc agcattgctc
    30841 gctccatctt cctgaacata tggaagacac cgcaaatcgc ctgattttac cttgtcatta
    30901 attaaggaaa tgaaaatgac atttatgaaa ggactacccc ttttgttgtt ggttgccagt
    30961 ctgtgcagcc acgctgcact acaacccgat cgcactcgta ttgtgtttaa cgctaatgat
    31021 aaagctacca gtctgcgggt ggataatcgt agcgataaac tcccctacct cgcttattcc
    31081 tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc tggtcgccct gccgccgata
    31141 caacgtctgg aaccgaaagc aacaacacag gtacggatag taaagcaagc ctcgaccaca
    31201 aaattaccgg gcgatcgcga aacgctattt ttctacaata tgcgtgaaat tcctcccgcc
    31261 ccggaaaaaa acagcgacca tgccgttctg caagtcgcca ttcagagtcg tatcaaggtg
    31321 ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa aggtcgaact gcagttacag
    31381 gtcagccagc agggcaacca actgacactg aaaaatccta ccgcgtatta tctgaccatt
    31441 gcctacctgg gacgaaatga aaaaggcgtt ctccccggct tcaaaaccgt gatggttgcg
    31501 ccttttagta cggtcaacac aaacaccgga aattatagcg gcagccagtt ttatctcggt
    31561 tatatggatg attacggcgc gttacgcatg acgacgctca actgcagcgg acaatgccat
    31621 ttacaagccg tggaggcgaa gaaatgagtg ctggcaaggg agtgttgctc gtcatctgtc
    31681 tgttatttct gtcgttgaag tctgcgcttg ccctgaactg ctactttggt tcatccggtg
    31741 gctcggtaga aaaatcagaa gcgattcaac cttttgctgt accaggcaat gccaaactcg
    31801 gcgataagat ctgggaatct gacgatatta aaataagatc tgggaatctg acgatattaa
    31861 aattcccgtc tattgtgaca acaataccaa cggcaacttt gaaagcgaac atgtctacgt
    31921 atgggtaaac ccctacccgg gggtgcagga tcgttattat caactcggag taacttacaa
    31981 tggcgttgat tatgacgcca accagggaaa aagccgcatc gacactaacc agtgtatcga
    32041 cagtaagaac attgacattt atacccctga tgggatggca gaacaaaatt tgctcgggtg
    32101 atcccgctaa tattcacatg tcgcgaacct ttctcgctcg catgcggtta tacgtcaaaa
    32161 tacgagaaat gccgccgcat gattatcaaa gtacgcttag cgactatatc gtcgtgcaat
    32221 ttgacggtgc cggtagcgtt aacgaagacc ctactgccca aaacctgaaa tatcatatta
    32281 ctggtctgga aaacattcgc gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa
    32341 cacagatgat tgattttggt aaatttaatt tgctggatat tcgtcgccac acaatgtcga
    32401 aaacattcag tattaagacg acaaaatcac aaaatgacca atgcaccgac ggatttaaag
    32461 tcagttcctc cttttatacc gaagaaacgt tggttgaaga agataaagca ttgttgattg
    32521 gtaatggtct gaagctacgt ttgctggatg aaaacgcctc gccttatacc ttcaataaat
    32581 atgctgaata cgccgatttc accagcgaca tgttggtcta cgaaaaaacc tatacagctg
    32641 aactttcgtc cattgcaggt acccccatcg aggctgggcc cttcgataca gtggtgcttt
    32701 ttaaaattaa ctataactga tttgccatga tgccctttgc agccggaaag ggcatctctt
    32761 tcagaaaatt gctgattttc cccttttcct tgattgctgt tattttttcc tagcccggct
    32821 ttctttatgc tgtatcgata caccaaagag aaaataatga gggagcgaag gatgccagtt
    32881 ttgcagtggg gaatgttatg tgtgctatca cttctccttt ctattggttt cctcgcggta
    32941 catctcccgg cggcgctatt acttgggccg atgatcgccg ggatcatctt cagtatgcgc
    33001 ggaattactc tgcaacttcc ccgctccgct tttctcgccg cgcaggccat tcttggctgc
    33061 atgattgcgc aaaacctcac tggttcgatt ctcaccacgc tggccgccaa ctggccgatc
    33121 gtgcttgcta ttttactggt gacgctgctt tccagcgcca tcgtgggttg gttattggtg
    33181 cgctatagct cactgcctgg aaataccggt gcctggggtt cttctccagg cggcgcagcg
    33241 gcaatggtcg ctatggcgca agattacggc gcagatattc gcctggtggc gtttatgcaa
    33301 tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg tcacccgcat gatgctgggt
    33361 gataacgctg aagcggttaa ccagcaaatc gtctggttcc cgccagtgag cattaatctc
    33421 ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg gatgcatgct gcgtcttcct
    33481 tccggcacga tgctcatccc gatgctggcg ggcgcagtgc tccagtctgg tcagctcatc
    33541 accatcgaac taccggaatg gctgctggcg atggcgtata tggcaattgg ctggcggatt
    33601 ggtcttggtt tcgataaaca aatcttactg cgggcactac gcccgctacc gcaaatcctg
    33661 ctgtcgattt ttgctctgct ggctatttgt gcaggtatgg cgtggggact gacccggttt
    33721 atgcatattg attttatgac tgcctacctc gccaccagtc ccggcgggct tgataccgtg
    33781 gcggtcatcg ccgcagggag caatgccgat atggcgctta ttatggcgat gcaaaccctg
    33841 cgcctgttca gtattttgct gacggggcca gccattgcgc ggtttatttc aacctatgcg
    33901 ccgaagcggt cggcctagtg ctggcagcct ggacaccagt aaaacgggcg cgaggacaac
    33961 gtggttttct caatgatgcc gccacagcgt tcgcacagtt cgccatcacg atgaaaaacc
    34021 ttaaagcgaa acagcgcccc atgatgctta ttctcatcca cctgcccccg cgtagcgtaa
    34081 gaaaaccgag gaatctccag taacgcgtgt gcgagtgcat ccagttgcgc cgcattgaga
    34141 tctttcgctt tatgatttcc agtcaaccca acctgccaga ggatctccac ccgcaaataa
    34201 ttgccaagcc cagccagaaa cgcctgatcg agcagtaatc ctgcaaactg acggttacga
    34261 aagcgcggcg acaataaccg ttcctttacc acctccggcg tcagattcgg atccagcaca
    34321 tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt gttccggcct caacatctca
    34381 atatccgagg cgctataaag cagaatggtt ttgtcagccg tttgcagttt tacccgcaat
    34441 actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc gccagacgcc gtaaagctga
    34501 ttatggctgt agagcgttaa accgttggga aaatgagtta acaacgcctt accacgcgtt
    34561 tccacatggg taacgtgttg accgataagt tgtgattgat aagtttttaa ctgcgggaag
    34621 gcaaaccaga catcagttag tggtttgcct ttgatcgccg cctccaggtt atccgctgca
    34681 cggcggatct ccgggccttc aggcatggtg atatcctttt ttcattcttt attaagcggg
    34741 gttatgctgc gacaacaatc ccctttttgg caaatgtagc tcgtaatcgg cgggcgaacg
    34801 ccagagcgtg ctcgccgtcg ccatgcaggc agaccgtttg cgccgcgacc gttgcccatt
    34861 cgccggtgat acttttgact ctgccgtgtt gcaccatctc cagcgtttgc gccaacgcct
    34921 gttcttcgtt ttcaatcaac gcgcctggct gacttcgcgg caccagcgag ccgtcagcct
    34981 gataaccgcg atcggcaaac acttcctcgc gcgttgtcag gccatattgc ttgcctgcac
    35041 gaatcagctc gcttcctgcc agcccaacga gaaccaatgc cggatcgcaa gcgtataccg
    35101 ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc ctggttgtac aacatgccgt
    35161 gcggtttgac atgacgcatt acgccgcctt gcgcacgggc aatcgttgcc agcgcgccaa
    35221 tttgatacag cgtctgggcg taaacggttt ccggcggcag ctgcatggcg ctgcgaccaa
    35281 aattttccct gtcgggaaaa ctcgggtgag cgccaatcgc gacaccattt tttattgctt
    35341 cacgcacgca agcctgcatg gtttgcgcat cgcctgcatg aaatccacaa gcaatattgg
    35401 cagaggaaac cagcgttaat agctctgcgt cgctggcgca gccttcgccc agatcggcgt
    35461 tcaggtcaat tttcattgtg cagccgccac gctaattgtt caaaataacg ttgctgatct
    35521 tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa aatgaatcgg ctgaccgagc
    35581 ggaatttgcg ccagatggta catatcagcc tcaatgatac aggcaatacg tggataacca
    35641 ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc cgttatgcgg cacctgcacc
    35701 acgcccggta acaaaccgtg agataacagt tcgcgatcgg tgatgcgttt taaaatttgc
    35761 ccctgtaaac gatagcccat tcggttactt tgcgagctaa gctgccaggg cgaacgccag
    35821 aatgcatcct gcgaggcgcg atcgaactca tgatattccg gccccggcaa ggcgcgaatg
    35881 cggttgcccc acagcagctg tttaacgccc tgcgcttcca taaaatcacg cttcgatttg
    35941 ccaatcggga gtcagtcacc atccttcagt aaacggcctt ccagcccgcc aatccccact
    36001 ttgagatcgg tgctgcatga ccccattacc ggcggaacat caataccacc cgcgaccgcc
    36061 agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta aacgctggcc tgctttcatc
    36121 ggcaatcgcc agccggtcca gacggcgtta tcatccaacc gcgcttcgca acctgcaccc
    36181 gtcagggcaa accacccatc agtttcgaac tcaactgtta actgaccgag cgtgatctcc
    36241 agcgcggggg cattggcgtc attacccacc agcaggttag caatgcgcag cgcaggcata
    36301 tccagtgcgc cacagtggct gatacccgac tggcaaaaac cgtgacgacc gccatcctgc
    36361 acagtggtgt acatgcccgc acgaataatc ttcagcatat tccctccttc tgcggtacaa
    36421 atcgcacgct gtctcccgga cgtaataaga tgggttcgtc acgcgccgga tcaaacaggc
    36481 tgagtgaggt atgaccaatc aactgccagc cacccggcgt tgccagcgga taaacaccag
    36541 tctgcggccc gccgatcccg acagaacctg ccggaacgag caagcgcggt tcagcgcgcc
    36601 gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg gaagcccggt tgaaaaccta
    36661 aaaaccagac cacgtattcc acggaggagt gcaattcaac aacctgtttt tcgctcaacc
    36721 cgcaatacgc cgcgaccacc gccaaatccg gtccgcctgc accaccgtaa accaccggaa
    36781 tttcaataaa gcgagactca ggttccaggg cctcgctctc ctcccaccag cgttgcaaac
    36841 gctctatggc atccagcgcc agcgactcag gattacgcaa aatcaccgtg atattgttca
    36901 tgccgggaat ggcttcaacc acattcggca tatccaccag acgctgcgcc agtcgccaga
    36961 tccgtttctg gctagccagc gtcaccggcg gttccagttc cagcactacc gccgtttcac
    37021 ctatcagata acaacgcgct cgttgcactt tcgatccctc tgattattcg ttatgcagga
    37081 ttaggaatat caataaaggt cacatcaaga tcggtatttt cattcagcca ctcgctcaat
    37141 gcgcgaatac caccgcgttc agtggcatgg tgacctgcag cataaaaatg caatccctgc
    37201 tcgcgggctg aatgaatggt ctgttcagaa acttcgccag taataaaagc atccacgcca
    37261 aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg tgcaccaggc gacgcgctgg
    37321 accacctcag gtccggtatc gccacaccat aacggcttac gtcccagacg cgcttcaatc
    37381 caggaggcta gttccattcc cggcacaggc atggtcagct ctccccacgg caccaacggc
    37441 tcaatttcgc ccatgaccgt gatccccagt aacgccgcca gctgtgcgtt attgcccagc
    37501 tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt tgatatcatt cgccagcaac
    37561 gtttttaaac ggttacgctt cattccgcga atgaccggag actctccttt ccagaagtag
    37621 ccatgatgca caatgaccgc atccgcgccc agacgcactg cctcatcgag caaagcctgg
    37681 ctggcggtga caccggtaac aattttttgc accgtctctt tgccttccac ctgcaaacca
    37741 ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt cgttaatcag ttgttccagt
    37801 tcggtgtttt tcatcttcgc gccttccgta ttctgatagc tacaccgtta acatagcgtt
    37861 acatcgcctg ttcgtcgata gccaatcttt tgacatatta agaaacaaac ccgacgccaa
    37921 aagacgcaaa ttgtttatat catcacgctt cagctaatgt atatttatgc aatcagacag
    37981 catattgcat aaataatcag atctgtatca aagttttaac tttaaggaaa cacaagcacg
    38041 ccgcgcaact attacgtgac gttgccgtca attgtggaat tattatgaat aaacacgcat
    38101 cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat ctgggaatat tttagctttt
    38161 acggcatgcg tgccctgctg attctctatc tcaccaatca actaaaatac aacgatactc
    38221 acgcctacga gttatttagc gcctactgtt cgctggtgta tgtcacgcca atcctcggtg
    38281 gctttttggc ggataaagtt ctcggcaatc gcatggcggt gatgctgggg gcgttgttga
    38341 tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca tccgtcattc ctctatctgt
    38401 ccctggcgat tatcgtctgc ggctatggcc tgtttaaatc taacgtaagc tgtctgctcg
    38461 gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg tttctcgctg atgtatgcgg
    38521 cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg ttacgcccag gaagagtaca
    38581 gctgggcgat gggctttggc ctggcggcgg ttggaatgat cgcgggtctg gtcattttct
    38641 tatgtggcaa tcgtcatttc actcataccc gcggcgttaa caaaaaagta ctgcgtgcga
    38701 caaactttct cctgccgaac tggggatggc tgctggttct gctggtggca acgcctgcac
    38761 tgattaccgt gctgttctgg aaagagtggt cggtatacgc cttaattgtc gcgactatta
    38821 ttggcctggg tgtactggca aaaatttatc gcaaagcaga aaaccagaaa cagcggaagg
    38881 agctggggct gattgtgacg ctcaccttct tcagtatgtt gttctgggcc ttcgcacaac
    38941 agggcggtag ctcgattagc ctttatatcg accgcttcgt taaccgcgat atgtttggtt
    39001 ataccgttcc gaccgcgatg ttccagtcga ttaatgcctt cgcagttatg ctgtgcggtg
    39061 tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa tcgtaccgtg cgcatctggg
    39121 gaaaatttgc tcttggcctt ggcctgatga gcgccggatt ctgcattctg accttaagcg
    39181 cccgctggtc cgcaatgtat ggtcactctt ctctgccact gatggtgtta ggcctcgcgg
    39241 tgatgggctt tgcggaactg tttatcgacc cggttgccat gtcgcaaatt acgcgcattg
    39301 aaattccggg cgtgaccggc gtattaaccg gcatctacat gctgctttct ggcgcgatcg
    39361 cgaactatct ggcgggcgtg attgccgatc agacatcgca ggcttcgttt gatgcttccg
    39421 gggcgatcaa ctactccatc aatgcatata ttgaagtatt tgatcaaatt acctggggcg
    39481 cactggcgtg tgtaggactg gtactgatga tttggctgta tcaggcgctg aaattcagaa
    39541 accgcgcgct ggcgctggag tcataatagg ccagcaccga tgcctgatgc gacgcttgac
    39601 gcgtcttatc aggcctacgt atttcctgca atttattgaa tttgcacaaa tttgtaggct
    39661 ggattaggcg ttcacgccgc atccggcatc tgggctcgat tgcctgacgc gtctgttatt
    39721 tccccttccg cgccgcctca tacgctgcca acgtttgtac tctcgcctct ttgtgctcga
    39781 ctatcggttg cggataatcc agcttcacac ctgctttctg cgcccacttc cacggctcat
    39841 gcaccgcttt ccctggtaca tcgcgcagct ccggtaacca tcgacgaata aactcgccct
    39901 cacggtcaaa tttctcgccc tgagttatcg gattgaaaat acgaaaatac ggcgcagcat
    39961 cagtacccgt agaagccgcc cactgccagc caccgttatt agctgccaaa tcaccatcaa
    40021 tcagctgcga catgaaatat cgctcgcctt cgcgccagtc gatcaacaaa tctttcacca
    40081 gaaaactggc tgtaatcatc cgtagccggt tatgcatcca gccagtgctg ttaagctgac
    40141 gcatagcggc atcaataatc gggtatcccg ttttgccttt ctgccaggcc tgtaaatgtg
    40201 cgggattgct ttgccactgc acacgatccg tccaggcaat aaatggacga tgtttacaca
    40261 acgaggggta atacgtcatc agatgacggt agaactcgcg ccagatcagc tcactaagcc
    40321 agacactacc ggccccaccg tccagcgcct gcggctgttc cgccaacaag cgatgcaaac
    40381 actggcgagg cgataacccg cccgttgcca agctggcgga caaacggctg gtgccttcca
    40441 ctgccggaaa atctcgttgt tgctcatact ctccagcacc gttctcgcaa aactggcgta
    40501 attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc agtatcgaaa gactgacgag
    40561 gataattcag cgtaatggat ggcgcgggct ttatcgatcc gctactacga acttttggtg
    40621 cagcgacgca ctccggcatc ccttcccgca gccgtttcag ccaggcattc ttaaaaggcg
    40681 taaagacttt gtacatctcg tgattgccgg tcatcaccgc ccccggcggc aggatcacgc
    40741 tgtcatcaaa tccttcacac accacgttac gcagcgctct ttcaacgtcc acatcccgcg
    40801 ctcgctcatt cacctcatac tgatagttat aaaaaagatg agtaacgctg ttttccgcgc
    40861 acacctgttt aactatttcg acactggcga caaagtcatc cacttcacgg aacaataaag
    40921 gaataccttt ttccgccagc gcgatttgta gcccattcag ttgagcattg ataagttcag
    40981 cctgacgcgg cgacatgtta tgcgtctccc acttgcgtgg tgtagcgata tacagcgcca
    41041 gcacgcgtgc agacgaattg cggcaggcgg cagccagtgc gagattatcg tgcagacgta
    41101 aatcctggcg aaaccaggcc agatgggtag tcataaaact cctgaatggc gcaagttaat
    41161 cacacgcggc caaagttcga aataacgctg ccctgaaagc caggcgtcag gataaagcgc
    41221 catatagtgt ttgatacgca tcagcggcgc aagaagtggt tgctcgccac ggcgataact
    41281 gtcgataagc gcagccagct gctggcgttc tgtggaatca atatgcgggc gaaaataacc
    41341 ctgaacgtgc atcaaaacgt tggtgtgatc gcgcgggttg gcgggatgag aaagcaaaac
    41401 aattacgcgt tggcggtact ggttgtaaaa atcatccaga ttttgccact gatgaatatc
    41461 agccacaaac gggccaagtt tgcggtactc gggctgagaa tgggccagaa agacgagttt
    41521 atagcggctg tgataatcga gaagcgcccc gcgcgttaag ccatgttctt tcagtcgatg
    41581 taactcatga agggcgaaga tacggattag ggtgctgtca tcaaatcgtt gtagattcat
    41641 tactgctcct gaacggtata caggagtaag tgtaggcgtt ggtaaatgat gtttgtgaag
    41701 ttaatccgct agcagggcaa gattacgaaa gcgcgctccc cgcaagcaac taatcaaggt
    41761 agttaatcat ggctgaacac gatattacgc cagaagtcac tatttccaaa acacagcgcc
    41821 gctataaagt cggctatgtc agagcccgcc accaggaccg caaaaccggt atagcccggt
    41881 attattcaca acatcccagt ctgcacctta agggcgactg gctgaaagag gccggatttg
    41941 ataccggacg gggtgtcacg gtgaagattt cggagggggg cctgaccatc atcgctgaca
    42001 gtgacgaggt gcaggaactg cgggatggga tgtttagtgt gctgaacgaa agttaaatat
    42061 taaaaaagct gggaagattt aaaaccttcc tagcttttta ttctgtctta ttataatgga
    42121 tactcatcat cttccgatga atttataaat tgctccaagt ttacatgaag agtacttaac
    42181 ctagttttta actcattaag tgaagcgata atttcatatt ttccaacata atcatatgat
    42241 ggatcgtatt cattatctat gtcagaagct aaaaatgatg atattcgtag tttaaattta
    42301 gcttcatctt tattacattc attggcataa ggtgaatgcc ctatcatagc atattcatct
    42361 aatctaaaag gataagatgg ttttaattca tcgatcaatg aaatttcaat ccatgttcta
    42421 tatccaggca ttaatggact aatttctttg agcaaaacct ttccacttaa taatgtattg
    42481 agattgttca ttattatgtc tcccacggga aaaatatatg tctaacactt ccaccatgtt
    42541 gatgaacttg taaatgtggt aaatcaccat caggatttcc tggtccatga cctgacactc
    42601 tatatgaagg ttttgttaca gaaggatctt ttatatcatc ccaatgatat gaacctgctt
    42661 ttgttttatt ttcagtaaga tattccatta agttcttagt gctggggcct gactcacctg
    42721 tcatattttt ataattatga cctaaatatc tatccataaa caattgttca gcgtcacttc
    42781 tagacctaac agtaactttg gtttcactac tatctaataa acgattcgct ttttggttac
    42841 taacagcaca tggagttaat ccaagtggat cagcatattt tagaggatta ggtgcataac
    42901 tgtagagatt taaaccgcct ctcaacccta tcgggtcagt taccatgaac cgcccgacat
    42961 ccggatcata gtacctgtgc agattgtagt gcagccccgt ttccctgtcg aggtactgtc
    43021 cctggtagcg caggttctgc tccaccccgc cgtggtcctt ctcctgtatc gtgttacccc
    43081 agacctgata ccccgtctcc cacacgattt tcccgtcgct gtccgtcatc tcttccggcg
    43141 cgccgttcac atccgtgtga aaatacagta tccggttcgg gccctctttg cccgccttat
    43201 ccacccgcgc cagcggttca taactcccct ggtccctgta gatgtacagg ctgcctgttc
    43261 cctccggcct gctctcctgc gccagccgca ttccgtccca taaaaatttt gtccggttgt
    43321 acggcttacc gtcagggctg atgtgctgtt tttccgtccg cctccccagt gcatcgtaca
    43381 cataccggta tgtcccccgc gctgtcgtca cttccaccat ccggtgttcc gcatcataac
    43441 ggtaactctg cgttccgctc cggccctcct tttccaccgc acggccaaat tcgtcataac
    43501 ggtagtgcac accacggtaa gacatcagcc ggttgaacgg caccacgctg ttttctgccg
    43561 tggccctttc ccccgcgcgc ctccccagca ggtttcccgc cgcgtccacc tccgtttccg
    43621 gctggtgcca caccgggtgc cactgcgcct gctccacccg ccccagcggg tcgtgcgtct
    43681 ccgtgatatg cccctgcagg tcatacacat agcgccattt gccgccctga gggtcggtca
    43741 tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt cgtcacctgt ccggcttcgt
    43801 cctggtacgc cgtcacctga ttctgcgcat cccactggtg ataacgtgtg cgccccagtc
    43861 cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc cagccggtac tgttcaccgc
    43921 tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc cggaccttca taccagcccc
    43981 actggtaata atacccttcg ccgcccggct ggcggtgcag cgtcatgcag tggttttcgt
    44041 cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta cacgccgttc aggtggccgg
    44101 cctcgtcata gctgtaacga cgcagcagta aaggtgcagt ctcagggaaa tgctgataaa
    44161 tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata cagacggatg cacggacgct
    44221 gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc gtagtacaga ttcagacagt
    44281 tgctgttacg gtcgcccagc attttcagcc gctggcgctg cgggtggtgc gggtcagcct
    44341 caaacagaaa acactgcccg tcatcgctgc tgataagcca gtgcccctgc tcaccacgcc
    44401 ttaccgccag cccttcaccg gggctgtaaa acccgccccc cggcagtacg gcttccatat
    44461 ccaagcgtcg ccccatgccc gacacaaagg tcatgcagtt ttcgtctgcg ctacccggtg
    44521 tgcacacgag gctgacttca tacattacac tccagccctg accaaacatt ccttccgtac
    44581 gttcgtcacg actgctgtac acgcgctgcc agtccagcgg aaagtggccc ggcagactga
    44641 aatcgaagtc atcgtcatcg gccaggtatt tggccccggt ggcggcattg accggatgcg
    44701 gaagggcact ggtggccttc tgcgtcagca tgctcatccc gacactggca gcaaaacagg
    44761 cgattttaga aagcattttg cccgggcgaa gaaacatcag cgccacggtt gccaccaggg
    44821 ttatctgcga cctgccgctt ctgatatccc tgaccaccag taacggaccg ccaatgcgca
    44881 catccggtga cacaaacgcc ccgttccccg gctcattcac caccctggcc tcgcaggtac
    44941 agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg cacatcccgc gcgccttccg
    45001 cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa cagattactg atgctgccaa
    45061 taaccgcatt cactccctga aaggcggttt ccagcatacc gcccggattc agcaggttaa
    45121 gggccgttcc cagggggtca tccagaaagg cggctttttt ttgtgcgaga aactgcgtca
    45181 gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg ggtcggggtg gatcccgggg
    45241 ccgcactggc cacagtgggc tgccatagtt ccgacacaaa atgtgtcacg ccatccacca
    45301 tttcagaacg cccttcttcc gtactgagct ggtcgtgcag agcccgggac atcagcacag
    45361 gccctgtcac cgggttaacc gccagggcta tggcccccgc catttccaga tattccccgg
    45421 ccacatcccg gaacgttctg gcatcctcct gacgctgcgc ttcttcctgt tctgccttcg
    45481 cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc agtcgccgct gcccgcgcgg
    45541 caggtttgct gttgatccat gtgtcaccag agccggtgag gatgtaacct tcaatctgag
    45601 gcgggaaaag catatttgcg agggctgaac aggcttcgct gaccttttct gtcacgccgg
    45661 tgtacaccat cgccacaccc gccacaaaac cggtcagcac gacggccgtg caccccatcg
    45721 tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac aatgagggca tcaataacga
    45781 catttgcggc cacctccagc accgccccca gcacatctgc cataaacgac gtatgcagca
    45841 gggcatcacc ctgtcttgcc gcccagaatg cctcttccat gtcccgttcc ctgtatgatt
    45901 aattcgtcct gacttcctgc cgcatcctga agcgcagatt ctttttaatt tcagcccagc
    45961 gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta ggtgaaggcc atcatgaccc
    46021 gctcacccgg caatacaaat gccacctgac actggtacac ctgctcgtta ccccggagaa
    46081 actggctctg tatttccaaa ccgcctcgtt gagataatat tccgtcataa tgtaaatagt
    46141 ccgttttaat aaaggaatta tttattgaca ggttctgttt ttttaatcat gaatgtaata
    46201 cccatcaccg ctttacagca cactttatct ttccagtcat aatcactaat aatattaagc
    46261 aattagcaat atttacaacg accagagcgg atacatagtt atgtatttaa caatcccaat
    46321 atattcacta tgcaaataa
//
LOCUS       DAJTPG010000022        45524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTPG010000022 DAJTPG010000000
VERSION     DAJTPG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            95..2248
                     /locus_tag="OO230_001281"
     CDS             95..2248
                     /locus_tag="OO230_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5691108.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNSTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="OO230_001282"
     CDS             2301..4031
                     /locus_tag="OO230_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691109.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO230_001283"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO230_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5691110.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="OO230_001284"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="OO230_001284"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5691111.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="OO230_001285"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="OO230_001285"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5691112.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO230_001286"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO230_001286"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5691113.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="OO230_001287"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="OO230_001287"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5691114.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="OO230_001288"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="OO230_001288"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5691115.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="OO230_001289"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="OO230_001289"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5691116.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="OO230_001290"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="OO230_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5691117.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO230_001291"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO230_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5691118.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO230_001292"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO230_001292"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5691119.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO230_001293"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO230_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5691120.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO230_001294"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO230_001294"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5691121.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO230_001295"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO230_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5691122.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO230_001296"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO230_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5691123.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="OO230_001297"
     CDS             16289..16828
                     /locus_tag="OO230_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5691124.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="OO230_001298"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="OO230_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5691125.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="OO230_001299"
     CDS             complement(18187..18534)
                     /locus_tag="OO230_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5691126.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO230_001300"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO230_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5691127.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO230_001301"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO230_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5691128.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO230_001302"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO230_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5691129.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO230_001303"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO230_001303"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5691130.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO230_001304"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO230_001304"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5691131.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="OO230_001305"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="OO230_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO230_001306"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO230_001306"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5691132.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO230_001307"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO230_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5691133.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO230_001308"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO230_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5691134.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO230_001309"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO230_001309"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5691135.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO230_001310"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO230_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5691136.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="OO230_001311"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="OO230_001311"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5691137.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="OO230_001312"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="OO230_001312"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5691138.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="OO230_001313"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="OO230_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5691139.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO230_001314"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO230_001314"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5691140.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="OO230_001315"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="OO230_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5691141.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="OO230_001316"
     CDS             complement(39992..40447)
                     /locus_tag="OO230_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691142.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="OO230_001317"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="OO230_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO230_001318"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO230_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5691143.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="OO230_001319"
     CDS             complement(41290..41967)
                     /locus_tag="OO230_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5691144.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO230_001320"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO230_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5691145.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO230_001321"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO230_001321"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5691146.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="OO230_001322"
     CDS             complement(43173..43631)
                     /locus_tag="OO230_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5691147.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="OO230_001323"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="OO230_001323"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5691148.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..>45524)
                     /gene="adrA"
                     /locus_tag="OO230_001324"
                     /gene_synonym="dgcC"
     CDS             complement(44577..>45524)
                     /gene="adrA"
                     /locus_tag="OO230_001324"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5691149.1"
                     /translation="ASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIY
                     NLKTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTL
                     ELTGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMT
                     GVYNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQ
                     LQITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRIS
                     VGVAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11835 a        11882 c        11133 g        10674 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatagc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgataccagg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaaacc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagc
//
LOCUS       DAJTPG010000023        43998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTPG010000023 DAJTPG010000000
VERSION     DAJTPG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            37..111
                     /locus_tag="OO230_001325"
     tRNA            37..111
                     /locus_tag="OO230_001325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69..71,aa:Gln,seq:ctg)
     gene            complement(265..1440)
                     /gene="ubiF"
                     /locus_tag="OO230_001326"
     CDS             complement(265..1440)
                     /gene="ubiF"
                     /locus_tag="OO230_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5691150.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1586..3010
                     /gene="miaB"
                     /locus_tag="OO230_001327"
     CDS             1586..3010
                     /gene="miaB"
                     /locus_tag="OO230_001327"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5691151.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3163..4203
                     /locus_tag="OO230_001328"
     CDS             3163..4203
                     /locus_tag="OO230_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5691152.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4200..4667
                     /gene="ybeY"
                     /locus_tag="OO230_001329"
     CDS             4200..4667
                     /gene="ybeY"
                     /locus_tag="OO230_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5691153.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4757..5635
                     /gene="corC"
                     /locus_tag="OO230_001330"
     CDS             4757..5635
                     /gene="corC"
                     /locus_tag="OO230_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5691154.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5660..7198
                     /gene="lnt"
                     /locus_tag="OO230_001331"
     CDS             5660..7198
                     /gene="lnt"
                     /locus_tag="OO230_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5691155.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7595..8503
                     /gene="gltI"
                     /locus_tag="OO230_001332"
     CDS             7595..8503
                     /gene="gltI"
                     /locus_tag="OO230_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5691156.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8673..9413
                     /gene="gltJ"
                     /locus_tag="OO230_001333"
     CDS             8673..9413
                     /gene="gltJ"
                     /locus_tag="OO230_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5691157.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9413..10087
                     /gene="gltK"
                     /locus_tag="OO230_001334"
     CDS             9413..10087
                     /gene="gltK"
                     /locus_tag="OO230_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5691158.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10087..10812
                     /gene="gltL"
                     /locus_tag="OO230_001335"
     CDS             10087..10812
                     /gene="gltL"
                     /locus_tag="OO230_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5691159.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10930..11865
                     /gene="rihA"
                     /locus_tag="OO230_001336"
     CDS             10930..11865
                     /gene="rihA"
                     /locus_tag="OO230_001336"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5691160.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11949..13619
                     /gene="hscC"
                     /locus_tag="OO230_001337"
                     /pseudo
     CDS             11949..13619
                     /gene="hscC"
                     /locus_tag="OO230_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13679..15130)
                     /gene="djlC"
                     /locus_tag="OO230_001338"
     CDS             complement(13679..15130)
                     /gene="djlC"
                     /locus_tag="OO230_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5691161.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15127..15834)
                     /locus_tag="OO230_001339"
     CDS             complement(15127..15834)
                     /locus_tag="OO230_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5691162.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15936..16490
                     /locus_tag="OO230_001340"
     CDS             15936..16490
                     /locus_tag="OO230_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5691163.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16500..17927)
                     /locus_tag="OO230_001341"
     CDS             complement(16500..17927)
                     /locus_tag="OO230_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5691164.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17924..18631)
                     /locus_tag="OO230_001342"
     CDS             complement(17924..18631)
                     /locus_tag="OO230_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5691165.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18795..19772
                     /locus_tag="OO230_001343"
     CDS             18795..19772
                     /locus_tag="OO230_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5691166.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19842..20324)
                     /locus_tag="OO230_001344"
     CDS             complement(19842..20324)
                     /locus_tag="OO230_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5691167.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20559..23141
                     /gene="leuS"
                     /locus_tag="OO230_001345"
     CDS             20559..23141
                     /gene="leuS"
                     /locus_tag="OO230_001345"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5691168.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23156..23737
                     /gene="lptE"
                     /locus_tag="OO230_001346"
     CDS             23156..23737
                     /gene="lptE"
                     /locus_tag="OO230_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5691169.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23737..24768
                     /gene="holA"
                     /locus_tag="OO230_001347"
     CDS             23737..24768
                     /gene="holA"
                     /locus_tag="OO230_001347"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5691170.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24770..25411
                     /gene="nadD"
                     /locus_tag="OO230_001348"
     CDS             24770..25411
                     /gene="nadD"
                     /locus_tag="OO230_001348"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5691171.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25435..26046
                     /locus_tag="OO230_001349"
     CDS             25435..26046
                     /locus_tag="OO230_001349"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5691172.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26306..26623
                     /gene="rsfS"
                     /locus_tag="OO230_001350"
                     /gene_synonym="rsfA"
     CDS             26306..26623
                     /gene="rsfS"
                     /locus_tag="OO230_001350"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5691173.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26627..27094
                     /gene="rlmH"
                     /locus_tag="OO230_001351"
     CDS             26627..27094
                     /gene="rlmH"
                     /locus_tag="OO230_001351"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5691174.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27125..29026
                     /gene="mrdA"
                     /locus_tag="OO230_001352"
     CDS             27125..29026
                     /gene="mrdA"
                     /locus_tag="OO230_001352"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5691175.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29029..30141
                     /gene="mrdB"
                     /locus_tag="OO230_001353"
                     /gene_synonym="rodA"
     CDS             29029..30141
                     /gene="mrdB"
                     /locus_tag="OO230_001353"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5691176.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30152..31240
                     /gene="rlpA"
                     /locus_tag="OO230_001354"
     CDS             30152..31240
                     /gene="rlpA"
                     /locus_tag="OO230_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5691177.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31379..32590
                     /gene="dacA"
                     /locus_tag="OO230_001355"
     CDS             31379..32590
                     /gene="dacA"
                     /locus_tag="OO230_001355"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5691178.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32701..32964
                     /locus_tag="OO230_001356"
     CDS             32701..32964
                     /locus_tag="OO230_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5691179.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33065..33706
                     /gene="lipB"
                     /locus_tag="OO230_001357"
     CDS             33065..33706
                     /gene="lipB"
                     /locus_tag="OO230_001357"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5691180.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33965..34918
                     /locus_tag="OO230_001358"
     CDS             33965..34918
                     /locus_tag="OO230_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5691181.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35127..36092
                     /gene="lipA"
                     /locus_tag="OO230_001359"
     CDS             35127..36092
                     /gene="lipA"
                     /locus_tag="OO230_001359"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5691182.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36193..36396)
                     /gene="tatE"
                     /locus_tag="OO230_001360"
     CDS             complement(36193..36396)
                     /gene="tatE"
                     /locus_tag="OO230_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5691183.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36525..37313)
                     /locus_tag="OO230_001361"
     CDS             complement(36525..37313)
                     /locus_tag="OO230_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5691184.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37406..37789
                     /gene="crcB"
                     /locus_tag="OO230_001362"
     CDS             37406..37789
                     /gene="crcB"
                     /locus_tag="OO230_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5691185.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37843..38052)
                     /gene="cspE"
                     /locus_tag="OO230_001363"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37843..38052)
                     /gene="cspE"
                     /locus_tag="OO230_001363"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5691186.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38227..38787)
                     /gene="pagP"
                     /locus_tag="OO230_001364"
     CDS             complement(38227..38787)
                     /gene="pagP"
                     /locus_tag="OO230_001364"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5691187.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39376..40761
                     /gene="dcuC"
                     /locus_tag="OO230_001365"
     CDS             39376..40761
                     /gene="dcuC"
                     /locus_tag="OO230_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5691188.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40802..41143)
                     /locus_tag="OO230_001366"
                     /pseudo
     CDS             complement(40802..41143)
                     /locus_tag="OO230_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41481..41990)
                     /locus_tag="OO230_001367"
     CDS             complement(41481..41990)
                     /locus_tag="OO230_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5691189.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42153..42398
                     /locus_tag="OO230_001368"
     CDS             42153..42398
                     /locus_tag="OO230_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5691190.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42373..>43998
                     /locus_tag="OO230_001369"
     CDS             42373..>43998
                     /locus_tag="OO230_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5691191.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAEGVAMFNART
                     GRETEMCGGKLSFDDVFEREYARTIVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGG
                     SLKGAKNVYYNMALMNAGVKKVVVRFDPQQLHST"
BASE COUNT        11074 a        11006 c        10976 g        10942 t
ORIGIN      
        1 tttattcaag acgcttacat cgtaagtgca cccagttggg gtatcgccaa gcggtaaggc
       61 accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc acattaaaaa
      121 agctcgcttc ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat gcgatgttga
      181 cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac ctacggctgg
      241 cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg cctgacgttt
      301 caacacgcca gcacgctctg ccgccattaa cccgagatta cgcacaaaac gcagcggcgg
      361 cagattatta ctgaatccgg cataaaacag atccatacca ctttgcataa tgaagttatc
      421 cgccatgcgc cgcatctggt aacgcttgag gacaggataa ctggcccacg cttcaccgta
      481 gctgcgggca ttcaccagaa catcaatcag ggcatcgaca tcacgataac caagattcac
      541 cccctgcccc gccagcggat ggatggtatg cgcggcatcg cccaccagcg caagccccgg
      601 ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac gcaccagcgg caagcggtgt
      661 aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc gcctggagtt gcgccatatt
      721 catattctgc aactggcgaa tacgcgtcgg agagtcatac cacaccagcg atgcccagtt
      781 atcaaacaac ggcagaaacg cacgcggtcc gtccggggta aattgctgcc aggtgctgtc
      841 gccgggatcg ttctcacact gtacgctaat caacatgcac gactgcgcat actgccaggc
      901 atgaacgcca attcccgcca tctgccgcac ctgagaattt gcgccgtcgg caccaatcac
      961 cagtttcgcg cggatcgttt caccgccctt cagttccagc tcctgaagat cattatctcg
     1021 atgtaatgca atcagcgagg ttggcacacg taacgttact ttcggatgcg cttccagcgc
     1081 ctgccacaac gcctgttgca ggactgtgtt ttccaccatg tagccaagca acggcaactt
     1141 taattcggct gcgtcaaata ccacatgtgc cgtttcccac tcccaggttt ccagtctgcg
     1201 gtaaggatgg caacgcatag cctgtactgc atcccagacc cctaaccctt taagcaatga
     1261 taccgaagcc gcgctgatcg ccgagatccg cacgtccggt tggctgtcag cgacaaacgg
     1321 tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg tgctgtgcca gccccagcgc
     1381 cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt gatttgtcat
     1441 ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc ccaagggctg
     1501 ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc tgcattcctg
     1561 gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa cctggggctg
     1621 tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcct cccacggcta
     1681 tcaactgacc gacgtggcgg aagaagcgga tgtgctactg ctgaacacct gctcaatccg
     1741 cgagaaggct caggaaaaag tcttccatca gttgggtcgc tggaaactgt taaaagagaa
     1801 gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat
     1861 tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc accgtctgcc
     1921 ggagatgatc aactccgtgc gcggcgaccg cagcccggtt gtagatatca gcttcccgga
     1981 aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc
     2041 catcatggaa ggctgcaata aatattgcac ctactgcgtg gtgccttaca cccgtggtga
     2101 agaggtaagc cgtccgtctg acgatattct gtttgagatt gcccagcttg cggctcaggg
     2161 cgtgcgtgaa gtcaacctgc tcggtcagaa tgtgaatgcc tggcgtggtg agaactacga
     2221 cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga
     2281 tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca tcgaagtgta
     2341 ccgcgacacg cctgagctgg tgagcttcct gcatctgccg gtacagagcg gttccgatcg
     2401 cattctgaac ctgatgggac gtacccatac ggcgctggag tacaaagcga tcatccgtaa
     2461 actgcgtgcg gcgcgtccgg atattcagat cagttctgac ttcattgttg gcttccctgg
     2521 cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca atttcgacat
     2581 gagctacagc tttatcttct ctgcacgtcc aggaacgcct gccgccgata tggttgatga
     2641 tgttccggaa gaagagaaga agcagcgtct gtatattctg caagaacgta tcaatcagca
     2701 agcgatggca tggagccgtc ggatgctcgg caccacccag cgtattctgg tagaagggac
     2761 ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa aataaccgcg tggtcaactt
     2821 cgagggcaca ccggatatga tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc
     2881 gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa atgggtctgc gcatggcaga
     2941 aacaccggag tcggtgattg cccgcacccg caaagaaaac gaccttggcg tgggttatta
     3001 tcagccgtaa ttctcaggcc tgccgttccg gtgggccttg ttttttctct ctttatcccc
     3061 atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct tataggccgg
     3121 tgaatgttca gcttccggcg gcatacagat aagaggaacg gtttgaacat agacactcgc
     3181 gaaatcaccc tggagccagc agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat
     3241 gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg cgataaccac
     3301 tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct gcgtagcctg
     3361 tatgtcgata ctgccccgat gcgcggtcag attcaggata tcgaaccgga acagatccac
     3421 cttgcgatta aagaagcgcg ggtactggag caaagcgcgg agagcgtgcc ggagtacggc
     3481 aaagcggtca atatcaaaac taaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg
     3541 cagtacatcg ccaatattct cgaccatgac attaccttcg gcgttggccc ggcgggtacg
     3601 ggtaaaacct acctggcagt ggctgcggca gttgatgccc tggagcgtca ggaaattcgc
     3661 cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt cctgcctggc
     3721 gatttaagcc agaaagtaga cccgtatctg cgcccactgt acgacgcgct gtttgaaatg
     3781 ctgggctttg agaaagtcga gaaactgatt gagcgcaacg ttattgaagt cgcgccgctg
     3841 gcctatatgc gtggtcgtac gctgaacgac gcgtttatca ttctcgatga gagccagaac
     3901 actaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc aaaagcggtt
     3961 atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg cttacgtcat
     4021 gccattgaag tgctggccga tgtcgaagag atcagcttta acttcttcca cagcgaagac
     4081 gtggttcgtc acccggtggt ggcgcgtatc gttaacgctt atgaagcctg ggaagaagca
     4141 gaacaaaaac gtaaagcggc gctggcagca gaacgcaagc gcgaagaaca ggaacaaaaa
     4201 tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taattccggg ttaccggaag
     4261 agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa gagtcggaag
     4321 tgactattcg cgtggtcgat accgccgaaa gccacagtct gaatctgacc tatcgcggta
     4381 aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc atggagatgt
     4441 cgctactggg cgatctggtt atctgccgtc aggtggttga gaaggaagct caggagcaag
     4501 gcaaaccact ggaggcgcac tgggcgcata tggtggtgca cggcagtctg catttgttag
     4561 gttacgatca cattgaagat gacgaagcag aagaaatgga agccctcgaa acagagatta
     4621 tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt gccaggcatt
     4681 tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta actaacatga
     4741 gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga taagcaacaa
     4801 gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga aaaaccgtga
     4861 cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg aagatacgcg
     4921 cgatatgctc gaaggggtga tggacatcgc agaccaacgc gttcgcgaca tcatgatccc
     4981 ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat
     5041 catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc acattgaagg
     5101 gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag ccttcagcat
     5161 ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag accggatgct
     5221 gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat tcggtggggt
     5281 ttccggtctg gtgaccattg aagacatcct ggaactgatt gttggtgaga ttgaagacga
     5341 gtatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg
     5401 cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta gcgatgaaga
     5461 agtcgacact atcggtggtc tggtgatgca ggcgtttggg catcttccgg cgcgtggcga
     5521 aactatcgac atcgacggtt accagttcaa agtggcgatg gccgatagtc ggcgtattat
     5581 tcaggttcat gtcaaaatcc cggatgactc accccagcca aagctggatg aataaaaccg
     5641 aaactggata gataactaca tggattttgc ctcattaatt gaacgccagc gcattcgcct
     5701 gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg
     5761 gcctgcggcg attatttcgc tgatggggct tcaggcgttg acctttaacc gtcgcccact
     5821 ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg gtattaactg
     5881 ggtctatgtc agcatcgcga cctttggcgg gatgcctggc ccggttaaca tcttcctggt
     5941 ggtgctgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg tgctgtcgcg
     6001 tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc acccctgctc tctggcaagt
     6061 gaccgagttt ctgcgcggtt gggtgctgac tggcttcccg tggttacagt tcggctatag
     6121 ccagattgat ggcccgttaa aagggctggc accgctaatg ggcgtggaag ccattaactt
     6181 cctgctgatg atggttagtg gcctgctggc actggcgttg gtcaaacgca actggcgtcc
     6241 gctggtggtg gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca tccaatggtt
     6301 taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata ttccgcaatc
     6361 gctgaaatgg gacgaaggcc agcttcttaa tacgctaaag atttactaca acgcaacggc
     6421 accgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa ccgatctgga
     6481 aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgtgata aaggtagctc
     6541 gctggtaacc gggattgtcg acgcgcgtct caataagcag aaccgctacg atacctacaa
     6601 caccatcatc acgctgggta aaggtgcacc gtacagctac gaatcagccg atcgctataa
     6661 caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt
     6721 agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata tccagccgcc
     6781 gctgtcggca aatggtattg agcttactgc ggctatttgc tacgagatta ttctcgccga
     6841 gcaagtgcgc gataacttcc gcccggatac cgactacctg ctgactatct ccaacgatgc
     6901 gtggtttggt aaatctattg gtccatggca acacttccag atggcgcgaa tgcgtgcgct
     6961 ggagctggcg cgcccactgt tgcgcagcac caacaacggc attacggcgg tgattggccc
     7021 gcagggtgag attcaggcga tgatcccgca gttcgcccgc gaggtgttaa ccactaacgt
     7081 gacgccgacc accggactca caccatacgc acgtaccggc aactggccgc tgtgggtgct
     7141 gacggcactg tttggttttg ctgctgtgtt gatgagtctg cgtcagcgac gtaaataatc
     7201 ccttcttatc gtgccggaat acatcccata tttcggcacg ctcaccggat gcggcatcag
     7261 tgcattatcc ggataacaat attctcctat ccaactttgg cacatctatt gctttgttat
     7321 acaaggcaaa gcttgaacca gcatcagtac aacccggttg caccacagca atccaccggt
     7381 agcaccaaaa gagtgcattt taagaatttt gcttcagaat ggtgcggtgc acctcgcaaa
     7441 aacaaacaat aaaacatcaa tatctttaca tttagccaaa ctaaatgtta cctaacaggc
     7501 acaacactgc acaataaagt tgcagacgat aacaacacaa acactcacaa cgggtatcca
     7561 tgcgttctta acgcagaaga taaaggtgtt ggatatgcaa ttacgtaaac ctgccacagc
     7621 aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc
     7681 aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt gtcgtcggtc accgtgaatc
     7741 ttcagtacct ttctcttatt acgacaatca gcaaaaagtg gtgggttact cgcaggatta
     7801 ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac aaaccggact tgcaggtaaa
     7861 actgattccg attacctcac aaaatcgtat tccactgctg caaaacggca ctttcgattt
     7921 tgaatgtggt tctaccacca acaacgtcga acgccaaaaa caggcggctt tctctgacac
     7981 tattttcgtg gtcggtacgc gcctgttgac caaaaagggc ggcgatatca aagattttgc
     8041 cgacctgaaa ggcaaagccg tagtcgtcac ttccggcact acctctgaag ttttgctcaa
     8101 caaactgaat gaagagcaaa aaatgaatat gcgcatcatc agcgccaaag atcacggtga
     8161 ctctttccgc accctggaaa gcggtcgtgc cgttgccttt atgatggatg acgctctgct
     8221 ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg gatatcgtcg gcaagccgca
     8281 gtctcaggaa gcctacggtt gcatgttgcg taaagacgat ccgcagttca aaaagctgat
     8341 ggatgacact atcgctcagg tgcagacctc cggtgaagcg gaaaaatggt ttgataaatg
     8401 gttcaaaaat ccaattccgc cgaaaaacct gaacatgaat ttcgaactgt cagacgaaat
     8461 gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac taattacaag aaccaggggc
     8521 ggaaattcca gccctctcga ttgttacgta gcacggacag actatacgcc tgatggtcgt
     8581 tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa tcttcgaggg tagcagttaa
     8641 cgctgctacc ctcttttttc tggagtagat ttatgtctat agactggaac tggggtattt
     8701 ttttacaaca agccccgttc ggcaatacca cctatcttgg ttgggtctgg agtggttttc
     8761 aggtgacgat cgctttgtcg atctgcgcct ggattatcgc tttcctcgtc ggttcatttt
     8821 tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg tctgggtacg ttgtatgtcg
     8881 aactgttccg caacgtgccg ttgattgtgc aattctttac ctggtatctg gtgatcccgg
     8941 agctgctgcc ggagaaaatc ggcatgtggt ttaaggccga gctggatccc aatattcagt
     9001 ttttcctttc atccatgctc tgcctggggc tgtttaccgc cgcccgtgtt tgcgaacagg
     9061 tacgcgccgc tattcagtcg ctaccgcgtg ggcagaagaa tgccgcgctg gcgatgggcc
     9121 tgacgctacc gcagacttat cgatacgtac tgctgcctaa tgcttatcgc gttatcgtcc
     9181 cgccgatgac ttccgagatg atgaacctgg tgaaaaactc cgccatcgcc tcgaccatcg
     9241 gtctggtgga tatggcggcg caggcgggta aattgctcga ctactcggct cacgcctggg
     9301 agtcgtttac cgccatcacg cttgcgtatg tgttgattaa cgcttttatc atgctggtga
     9361 tgacgctggt tgaacgtaaa gttcgcctgc ccggcaacat ggggggcaaa taatgtacga
     9421 gtttgactgg agttccattg ttccttccct gccatatctg ctcgacgggc tggtgatcac
     9481 cctgaaaatc accgtcacgg cagtcgtgat tggtattttg tggggtacca tgctggcggt
     9541 gatgcgcttg tccagcttcg cgcctgtttc gtggtttgcc aaagcctacg ttaacgtatt
     9601 ccgctcgatt cctttagtca tggttttgct gtggttttac ttgattgtgc cgggatttct
     9661 gcaaaatgtg ctgggattat cgccaaaaaa tgatattcgt ttaatttcgg cgatggtggc
     9721 gttttcgatg tttgaagcgg cctactattc agagattatc cgtgccggta ttcaaagtat
     9781 ttcccgtggt cagtcgagcg ccgcgctggc gttggggatg actcactggc agtcgatgaa
     9841 actgattatt ctgccgcagg ctttccgcgc gatggtgccg ctgctgctca ctcagggcat
     9901 cgtactgttc caggatacct cgctggtgta tgtgttatct ctggccgatt tcttccgtac
     9961 cgcctcaacc attggtgagc gtgatggtac gcaggtcgag atgatcctgt ttgccggatt
    10021 tgtttatttc gttattagcc ttagcgcgtc gttgttggtc agctacttga aaagaaggac
    10081 agcataatga ttaccctgaa aaatgtttca aaatggtatg gtcactttca ggtgctgacc
    10141 gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg tttgcggccc gtctggttcc
    10201 ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac cagtgcagca aggtgaaatc
    10261 accgtcgatg gtatcgtggt taacgacaag aaaaccgatc tggcaaagct gcgctcccgc
    10321 gtcgggatgg tattccagca tttcgagctg ttccctcatc tgtcgattat cgaaaacctg
    10381 accctggcgc aggtgaaagt gcttaaacgc gataaagcgc ccgctcgtga aaaagcattg
    10441 aaattgctgg agcgcgtggg actttccgca catgccaata aatttccggc gcagctttcc
    10501 ggcggtcagc agcagcgcgt ggcaatcgct cgcgcattgt gtatggctcc tattgcgatg
    10561 ctgttcgacg aaccgacatc ggcgctggat ccggagatga tcaacgaagt gctggacgtg
    10621 atggtggaac tggcgaacga aggaatgacc atgatggtgg tgacccacga aatgggcttt
    10681 gcccgcaaag tggcgaatcg ggtgatcttt atggacgagg gtaaaattgt cgaagactcg
    10741 ccgaaagacg ctttcttcga tgatccgaaa tcggaccgcg caaaagactt cctcgcgaaa
    10801 atcctgcatt aatcactctg gcgcacatac ccaattgcgc gccatctcac gctttatcgt
    10861 ttcgcccctc gtatctgtta catcctggcg ttacccttgt cgcaaagaag cacacaacaa
    10921 ggagcaacaa tggcactgcc aattctgtta gattgcgacc caggtcatga tgacgctatc
    10981 gcaatagttc tcgcactcgc ctcaccagag cttgatgtca aagcaattac gtcttccgcc
    11041 ggaaaccaga caccagaaaa aaccttacgc aatgttctgc gtatgctgac cttgcttaat
    11101 cgcaccgata ttccggtggc aggcggcgcg gtaaaaccgt taatgcgtga gttgattatc
    11161 gcggacaatg tgcacggcga aagcggtctc gacggcccgg cattaccgga accgacgttc
    11221 gcaccgcaaa actgtacggc ggtggagctg atggcgaaaa cgctgcgcga aagtgcggaa
    11281 cctgtcacga ttgtttctac cggaccgcaa actaacgttg ccttgctgct caatagccat
    11341 ccggaactgc atagcaaaat tgcccgtatc gtgattatgg gcggcgcaat ggggcttggt
    11401 aactggacgc ctgcggctga atttaatatt tacgttgacc cggaagcggc agaaattgtc
    11461 ttccagtcag ggatcccggt ggtgatggcc ggtctggatg ttactcataa agcgcaaatc
    11521 cacgttgaag acaccgagcg tttccgcgcg attggcaacc ctgtttcaac cattgttgcc
    11581 gaactgctgg atttcttcct cgaatatcat aaagacgaaa aatggggctt tgtcggcgca
    11641 ccgctgcatg acccatgcac catcgcctgg ctgttgaaac ccgagttgtt taccactgtt
    11701 gagcgctggg ttggcgtgga aacacagggg aaatataccc agggtatgac ggttgttgat
    11761 tattattatc tgactggcaa taaaccgaat gccactgtaa ttgtcgatgt tgatcgtcag
    11821 ggctttgttg atttactggc tgatcgtctg aaattttacg cttaacactc gtcattgatt
    11881 aagatcacca cgcgttacct gggtggtctt aatctattta tctctttttc tttcaagata
    11941 taaatgcaat ggataatgca gaactcgcca taggtatcga tctcggcact accaatagtt
    12001 taattgccgt ctggaaagac ggtgccgcgc aattaattcc aaataagttc ggtgaatatt
    12061 taacaccatc cataattagc atggatgaaa ataatcatat tttagtcgga aaaccggctg
    12121 tatcacggcg tacttcgcat ccggataaaa cggcagcgtt atttaaacgt gcaatgggca
    12181 gtaataccaa ctggcggtta ggcagcgaca catttaacgc gccagaactg tcctctttgg
    12241 tattacgctc attaaaagaa gatgccgaag aatttctgca acgtccgatt aaagatgtgg
    12301 tgatctccgt tctggcttat ttcagcgatg aacaacgcaa gcatacccgt ttagcagcgg
    12361 acttagccgg gttaaatgcg gtacgcttaa ttaatgaacc cacagcagct gcgatggcgt
    12421 atggcctgca tacccaacaa aatacccgtt cgctggtgtt tgatctcggt ggcggcacgt
    12481 ttgacgttac ggtgcttgag tacgccacgc cggtgattga agttcacgcc tccgctggcg
    12541 acaacttcct cggtggcgaa gattttaccc atatgttggt cgatgacgtt ttaaagcgcg
    12601 cggatgtcac aaggaccacg cttaacgaga gtgaactggc agccttgtac gcctgtgtgg
    12661 aagcggcaaa atgtagcaat caatcgccaa tgcacattcg ctggtagtac caggatgaaa
    12721 cgcgggaatg cgaattttac gagaacgaac tggaagattt gtggttgccg ctgctcaatc
    12781 gcttgcgagt gccgattgaa caggcgttgc gcgatgcgcg tctgaagccg agtcaaatcg
    12841 acagtctggt gctggttggc ggcgcgtcac aaatgccgct ggtgcagcga atcgccgtgc
    12901 gtctgtttgg caaattaccg tatcaaagtt acgatccgag caccattgtc gcgctgggcg
    12961 cagcaatcca ggccgcctgc cgcttacgca gtgaagatat tgaagaagtg atcctcactg
    13021 atatttgccc ttactcgttg ggcgttgagg ttaaccgcca gggcgtttcc ggcattttct
    13081 cgccgattat tgaacgaaac accactgtgc ccgtgtcgcg tgtagaaact tattcaacca
    13141 tgcacccgga acaggattca attacggtta acgtctatca gggagaaaac cacaaagtta
    13201 aaaacaacat tctggtggaa tccttcgatg tgccgttgaa gaaaaccggg gcttatcagt
    13261 cgattgatat tcgctttagt tatgatatca acgggttgct tgaagttgac gtgcttctgg
    13321 aagacggcag cgttaagtcc agagtgatta accacagccc ggtaacattg agcgcgcagc
    13381 agattgaaga gagtcggacg cggttatccg cattgaaaat ttatccgcgc gatatgctca
    13441 tcaatcgcac ctttaaagcc aaactggaag agttgtgggc gcgggcgctg ggtgacgagc
    13501 gagaagagat tggccgggtg atcaccgatt ttgatgcggc gctgcagtca aacgatatgg
    13561 cccgcgtcga tgaggtcagg cggcgggcga gcgtttattt agccattgag acctcataat
    13621 cacggctgcc ggatgcggcg taaatgctgc atccggcatc ggcgcaaaaa acacatcgtc
    13681 aactaaacaa catcgcgatt ttgccgagag cataacctat aaccagcacc gttaaactga
    13741 ttttgataat gctggtaatg ctctgtggtt tttcaccctc atcatttttt tcttcatggt
    13801 tttcaggtgt tggctcatcg acatcttcat cctccggctc gagttttact ttcgtcaaca
    13861 gcatccgggt gataatggcc tctaatggat gagcatctcc gccttccagc cttgtattaa
    13921 gacgggccag catctcttgt ttcggaaacg cttcaagtac tacctgttcc gctacccgat
    13981 acaaatccag taacggtgaa tcttcttctg tctcgctacc gactctcgat ttccaccacg
    14041 cccatgcttt gcgtttgaag aataacgact caataaacga ttgacctaac ggcgataatt
    14101 ttccgctatg taaaatttcc gcccataaca gccgcgtccg ggcgctctca ccaggaacat
    14161 atttcagcgg ttgaccttgc gcatctacca tgctccgcgc ttcgataacc atcactaata
    14221 acgcgaaata atccgggcaa tgacgcttgc tccactgcaa caacaaatta tcagcttgtg
    14281 tagatgggta actttcccag tacgcgccga gatcagcaag caggctttcc ccctcgccgc
    14341 agtacaaacg ttgtgcacac tggtaatacc gggcatcttc gctttctggt tcttccgctt
    14401 gccactgttt ggctacagga atgagttcca ccatacctgt ttgcaccgag ctgtaccagc
    14461 gcaacagttt gcgatgtaac tttgcatcat caggaatgaa ccacggtcct tccatcgcca
    14521 aaaacgcagt gacatagtca ggatggtaac ggcagcaacg ttccagcgcg aagtaatatt
    14581 caacagtctg gttctgtaac gcgacgggat attccagaag gctaaggaaa tcgaaacaat
    14641 caccagcctg aatggcctca agaaaagcct ccctttcttc ctcatccatt ccttcgctgt
    14701 cattaaacga atgccagttc aaacgctctg ccaacaaaga agcacaattt aatgacagat
    14761 atcgcgcttc tatagcgatt gcacacagcg gccagcgtaa ttgatcgaca tcctccatgt
    14821 tccaggtatt taattgctga ataaatttct gccaggcgga gggctgaaaa cgatcacttt
    14881 ctgaatccag taatgtcctg aatgcacgta gtatttcatg ttcagcggtg gcatcttttt
    14941 cttcatcatc tgcttcctcg acaggattta ccgccagtcg cagcgcctct tcataggcct
    15001 ggcgtaatgc tttaaacccc tgcggatcgg tttcgggatg acacaacggt aagcgagcaa
    15061 gataagcctg gcggataatg tctatttgcg tcgtgctttc aatttcgagg atttgccaac
    15121 atgttttcat aggtcgctca catccttaag cgttaccggg cattcaggct ggacagttaa
    15181 tggctgccag ggaagcgtgg catagaaacg gtcgcttccc gctgccagct cttcgaacat
    15241 ccgactcccg gcgctatcgt atttgtacag ggttagcgcc tcgcgtagcg aagcgttgtc
    15301 ttcgcactga gaagattgcc agtagagacg ccctagtgaa taggcagaaa aatagtgcat
    15361 ccagctgtga tagtaatggt gagctcgtgc atagacgcgc gattgcagcc ataaactctc
    15421 ttcttccgtc agccagttgt tgagcacacc ggcccgtgac agaaatccca ttcgaacata
    15481 atcccaggct ttgattcccc cttctccaca gcacatagcg gtttgggcaa cgaattgggt
    15541 ataaatcaac cctctctccg agccaccagc aattaatgct ttccactctt caggggaaga
    15601 gcgttgccat gtcagatata agccagctaa atcattggca tgaccatcat cagtcatctg
    15661 ataaatagtc tgaattaaat catcacgatt atttattccc caggatgatg agagatcgaa
    15721 ttcttttatc ccggtataca tttttggcga gtcatgcgcg ggtttgcaac tctgattaag
    15781 tatttccatg ggcgcagaaa gcgcaaaaag aagatattgt tcttccttat tcatttttta
    15841 ataatccctt aagtgcatta actgactact tttttattat tgatgagtat aaacttgacg
    15901 ctttcaaaat aaaaagaaaa tcgaagcatt cacacatgaa taaaaaatta atgtatatat
    15961 tcgcaatttt tatagttgca gcaattacct gtattagcca acccaagaaa acgatgttgc
    16021 gtgataaagc catggtgaat tatgcctttg attatttaag atcaccaggc agtctaccgt
    16081 taaccacggc agccacggag ctttccgcga ttcatggtca ctcaacatcg caatatcgcc
    16141 ttggcgaatt ttatcttcat ggtagcgacg gtaaaccact ggattataca caggcgagat
    16201 actggtatga gcaatcagca gaacaggaaa atccgcgcgc acaaagcaaa ctggggtgga
    16261 tctacctgaa aggcctggga gtcaaacccg atacccgtaa agcaattctc tggtataagg
    16321 aagcagctga acaagggtat gctcatgctc aatatacttt gggtttgatc tacagaaatg
    16381 gcactggtat taatgttaac cattatgaat ctcaaaaatg gttaaaactg gccgccaaac
    16441 aacattacaa aaatgcggaa agattacttg ccggacttcc cgcacattaa cgtcgactgt
    16501 cacccgaaga gatggagtat tttccctatc aaaccggcaa agataaaaat gtagaaaata
    16561 attttgatga cgcccagaca gcccagcccg ccatcatcaa cgggcattgg ctgcggttgc
    16621 ggctccggct cgacggtttc ctgaccggta aaattcgccg cctgaattaa taatcgacga
    16681 ataattaatg cttccagtgg acaacgatct tctgcatcaa ggcggctcac aatcggcatt
    16741 aatgattctt ctgtcggttg gctggcctgc actaattgtt tggcaactaa atataatggc
    16801 cataagggat gttctgtatc acgcgtgagt gagatccgcg gcttacgtcc tttgtgcaaa
    16861 accatctcga ccagtgaacg ccccagcgca gagagttttt catcgctgag tatttcgtat
    16921 aaatgaaaac gcgtacgggc gctgtcgccg gggacataaa gtagcggttt tccctgatcg
    16981 ttaaccagat cacgcgcttc aatcatcatc accactaatg ggtaataata gacccgatgc
    17041 tgacggcacc attgcaacat taaagcatcc gcctgggtgg agggatactc gcgccagtag
    17101 taacaaagtt ctgccagcag gctttcgcct tctccacaat agacgcgttg tgcgtattca
    17161 taataacgcg catcttcatt atcaggttca gcttcccgcc acgaccgggc gtaatccagg
    17221 agttcgggga ttcccaggcg gcttgcgcta taccagcgga gtaaatttct gtgcagtttt
    17281 gcatcatcgg ggataatcac tgcgcgatgt tgcgctaaat acaatgtcag ccattctgga
    17341 tgcgatgacc aaatccgagc gaacatttgg taaaaatcga tggtgtcatt ttgcacggca
    17401 actggcagat gcagaatggt ctggaagtta aaaacattcc ccttcgcaat ggcatataaa
    17461 aaggattcca gttcttcttc gtctatttcc ccgacgtttt cctcctgcaa ccaccgcaat
    17521 ctttctgcta ataacaccac gcactcgaag gataaatggg cagtgttcat ggcgattgtg
    17581 cacagcgacc agcgtaattc atcaatctct tccatcgagc aataatttaa ttgctgaatg
    17641 aatcgctgcc aggcggaggg cagaaaacgt tcgctatcag aggcaagtaa cgcgcgaaag
    17701 gcgagcagaa tttcatgttc tgctacctca tattcttctg gttgccaaac agatttagca
    17761 ggcgactgcg caatccgtag cgcctcctca tacgcctgac gaagttgttt aaaaccctgc
    17821 ggatcggttt ctggatgaaa ggacggtaac agcgccagat aagcgcggcg gataatatcg
    17881 acgtcagtcg tttcctctat atcgaggatc ttccagcaat ttttcataag tcgctcatat
    17941 ccttaagcgt ttccgggcat gtggggtagt cagccagtgg ttgccaggac aaggtagggt
    18001 aatacgcatc atcactggca aataattgat aaaacatatc attgccggaa tcgtcgtatt
    18061 ctttacgcgc taagaattcg cgtaataaag gcagatgatc atcttcggga gactgccaat
    18121 attgccgacc aaaggtataa ccggcaaagt attgccgcca gttactataa taacgtagag
    18181 cccgtagatg aatacgcgac tggatccata gactttcttc ttcactcaac cagttattaa
    18241 gtacgcccat tcgacttaaa aaacccatcc ggacataatc ccatgcttta attcctccct
    18301 caccacagca ttcggctgtg ctggcgacaa attgcgcata agcctgacct tgctcattta
    18361 attcagcaag atagtcacgc cattcacatg gagaataacg aaaccagcgg tgataaaacc
    18421 ctgccaaccg agcggcatga ccgttctcga tcattcggtg aattatgcgc agcaggtcat
    18481 cacggtcatc aattccccat gaggaagaaa ggtcaaaaaa gcatgtacct aaatacattt
    18541 tgggggatga atgcgaaggt aaacagcatt cattgcgtat ttccatcgga gtggaaagtg
    18601 caaacaatat tttttgcgac cccatgtcca tataatttat ccttaaacat tcatttaatg
    18661 aatgtaaact acatttcgag gctcagttat ttatactttt ctgacgcaac ataatacgca
    18721 taaaataagg cttacgcaaa aatggaacac ataaaaatat aatatatttt atctacaacc
    18781 ataaggaaat gataatgatt tttacgtcaa gttgctgcga taatttatca atagatgaga
    18841 ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata tattgttggg ttttattata
    18901 atcgcgatag cgcaattgac tctccggacg acgaaaaggc cttttactgg ctgaagctgg
    18961 ccgctgagca aggtcattgt gaagcacagt attccttagg gcaaaagtat accgaggata
    19021 aaagccgtca taaagataat gagcaagcca tcttctggct gaaaaaagct gccctacaag
    19081 gccatacttt cgcttccaac gcccttggct ggatactgga tcgtggagaa gaccccaact
    19141 ataaagaagc ggttacctgg tatcagatag ccgcggagag cggaatgtct tatgcgcaaa
    19201 ataatcttgg atggatgtac agaaatggca acggagtcgc acaagactat acgctggcat
    19261 ttttctggta caaacaagct gcattacaag gccatagtta cgcacaagac aatctggccg
    19321 atctttataa agacggagaa ggcgttgctc aaaacaagac actcgccgca ttctggtatt
    19381 tgaaaagcgc acagcagggt aatcggcacg cccagtttca aattgcatgg gattataacg
    19441 ctggcgaagg ggtggaccag gactataagc aagcgatgta ctggtatctg aaggctgccg
    19501 ctcaggaaag cgtcggcgct tacgtcaaca tcggttatat gtataaacac ggacaaggcg
    19561 ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa agccgctgaa tgcaatgacg
    19621 ccactgcctg gtataacctg gccattatgt atcactacgg agaaggaaga cctgtcgatc
    19681 tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc
    19741 aggaaattcg tgagactgaa gctttactgt agaaataaaa acaagggccg gggaatttca
    19801 ttctgcccat attctcttta ttgctgtgcg aaatcagact attacggctc aaacgggcgt
    19861 ctctggaact ggtcataacc acatttcggg catagcgtca gcacttccgg tgtgtagatc
    19921 gggagatgga agtgacattt ctcgcagacc agatttccca gcccgaccac ttctccgctg
    19981 tgataaaccc catgatgatt gaggtcctgg aaaatttcgc gccattcaag ctgcgtttta
    20041 tcggtgatgt ctgccagctc ctgccacaag ctttctttaa tcacccgcat aaagacgctg
    20101 tcagattctt ctttcaggct ctcttcatag ctcatggcga actcttccag gtcacgtctg
    20161 acagctcgcg tcagctcatc gatctcggtt cgcgttaact cccctgtttt tattacgcgc
    20221 tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc cattgcgcag gcgttcgttc
    20281 agtgacgcga ccagttcacg gtaatattga gcaaccttgt tcatcgtttc atctcccggg
    20341 tcgttaactg actataataa tagacgttct ttgttccttg ctttgctaat acgcccacaa
    20401 actctgtaaa ttaatgtacg aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg
    20461 ctgttttgac gcggacgttt gggctatgct atgcggatct gaaaaaccac ctcaacgcta
    20521 catttgtagc cgtattgaaa acaggaccac tggctgccat gcaagagcaa taccgcccgg
    20581 aagagataga atccaaagta cagcttcatt gggatgagaa gcgcacattt gaagtaaccg
    20641 aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct tctggtcgac
    20701 tacacatggg ccacgtacgt aactacacca tcggtgacgt gatcgcccgt taccagcgca
    20761 tgctgggcaa aaacgttctg cagccgatcg gctgggacgc gtttggtctg cctgcggaag
    20821 gcgcggcggt gaaaaacaac accgctccgg caccgtggac gtacgacaac atcgcgtata
    20881 tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag ctggcaacct
    20941 gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat aaaaaaggcc
    21001 tggtatataa aaagacttct gcggtcaact ggtgcccgaa cgaccagacc gtactggcga
    21061 acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgaa cgtaaagaga
    21121 tcccgcagtg gtttatcaaa atcactgctt acgctgacga gctgctcaac gatctggata
    21181 aactggatca ctggccagac accgttaaaa ccatgcagcg taactggatc ggtcgttccg
    21241 aaggcgtgga gatcaccttc aacgttaacg actatgacaa cacgctgacc gtttacacta
    21301 cccgcccgga cacctttatg ggttgtacct acctggcggt agctgcgggt catccgctgg
    21361 cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt tattgacgaa tgccgtaaca
    21421 ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat actggcttta
    21481 aagctgttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga
    21541 tggagtacgg cacgggcgca gttatggcgg ttccgggtca cgaccagcgc gactacgagt
    21601 ttgcctctaa atacggcctg aacatcaaac cggttatcct ggcggctgac ggctctgagc
    21661 cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct ggcgagttca
    21721 acggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact gcgatgggcg
    21781 ttggcgagcg taaagtgaac taccgcctgc gcgactgggg tgtttctcgc cagcgttact
    21841 ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgcca accccggacg
    21901 accagctgcc ggtgatcctg ccggaagatg tggtgatgga cggcattacc agcccgatta
    21961 aagcagatcc ggagtgggca aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg
    22021 acactttcga cacctttatg gagtcctcct ggtactatgc gcgctacact tgcccagagt
    22081 acaaagaagg tatgctggat tccgaagcgg ctaactactg gctgccggtg gatatctaca
    22141 ttggtggtat cgaacacgcc attatgcacc tgctctactt ccgcttcttc cacaaactga
    22201 tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa acagttgctg tgtcagggta
    22261 tggtgctggc agatgccttc tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc
    22321 cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg
    22381 caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag aacaacggta
    22441 tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg tttatgatgt
    22501 ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggtgtggaa ggggctaacc
    22561 gcttcctgaa acgtgtctgg aaactggtgt acgagcacac agcaaaaggt gatgttgcgg
    22621 cactgaacgt tgatgcgctg actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa
    22681 ctatcgctaa agtgaccgat gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg
    22741 cgattatgga gctgatgaac aaactggcga aagcaccgac cgatggcgag caggatcgcg
    22801 ctctgatgca ggaagcactg caggccgttg tccgtatgct caacccgttc accccgcaca
    22861 tctgcttcac gctgtggcag gaactcaaag gcgaaggcga tatcgacaac gcgccgtggc
    22921 cggttgctga cgaaaaagcg atggtggaag actccacgct ggtcgtggtg caggttaacg
    22981 gtaaagtccg tgccaaaatc accgtcccgg tggacgcaac ggaagaacag gttcgcgaac
    23041 gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttactgta cgtaaagtga
    23101 tttacgtacc aggtaaactc ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg
    23161 atatctggca acactgttgt tatctctggc ggtgttaatc accgccgggt gtggctggca
    23221 tctgcgtgac accacgcagg ttccttccac tatgaaggtc atgatcctgg actcaggcga
    23281 tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt
    23341 gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttgggta aagtgagcat
    23401 cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc agatgatcat
    23461 gacggtgaat gcgaccgtgt tgatccccgg ccgtgatatc tacccgatta gcgccaaagt
    23521 cttccgttca ttcttcgata acccgcaaat ggcgttagcg aaagataacg aacaagacat
    23581 gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc tgccaagcat
    23641 ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg accacaacgg atactccggc
    23701 aacgcctgca cgcgtctcca ccatgctggg taactgatga ttcggttgta cccggaacaa
    23761 ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg taacgatcct
    23821 ctgttattgc aggaaagcca ggacgctgtt cgccaggtag ctgcggcaca aggattcgaa
    23881 gaacaccaca ctttttccat tgatcccaac actgactgga atgcgatctt ttcgttatgc
    23941 caggctatga gtctgtttgc cagtcgacaa acgctattgc tgttgttacc agaaaacgga
    24001 ccgaatgcgg cgatcaatga acaacttctc acactcaccg gacttctgca tgacgacctg
    24061 ctattgatcg tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc ctggtttact
    24121 gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc tcagcttccc
    24181 cgctgggttg ctgtgcgcgc aaaacagctc aacttagaac tggatgacgc ggcgaatcag
    24241 gtgctctgct actgttatga aggtaacctg ctggcgctgg ctcaggcact ggagcgttta
    24301 tcgctgctct ggccagacgg caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat
    24361 gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc
    24421 gcgttgcata ttcttcagca actgcgtctg gaaggcagcg agccggttat tttgttgcgc
    24481 acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc ccatacgcca
    24541 ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat gatgggcgag
    24601 gcgttaaatc gcttaagtca gccgcagtta cgtcaggccg tgcaactcct gacacgaacg
    24661 gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg cagagctgga agggttatct
    24721 cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata tgaaatcttt
    24781 acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctaa aacccgtgga
    24841 aacgctggcg aatttgattg gtctgacgcg ggtcacaatc atccctaata atgttcctcc
    24901 gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa cacatgcttg aactggcgat
    24961 tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg ccccctctta
    25021 cactgcgcaa acactgaaag agtggcggca ggaacaagga ccggacgtgc cgctggcgtt
    25081 tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg aaacgatact
    25141 cgacaatgca catttgatcg tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc
    25201 gcaataccag caatggctgg aagatcattt gacacataac ccggaagatc ttcaccttca
    25261 gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg cgaccatcat
    25321 ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta ttgccggaac cggtattgac
    25381 ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg catgatgcga
    25441 ctgtggttaa ttcgtcatgg cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat
    25501 gcgcccaccc ccctgaccgc acgcggtatt gagcaagcgc aaaatctgca tacactgcta
    25561 gatgatgttt cctttgactt ggttttatgc agtgaactgg aacgggcaca gcataccgcg
    25621 cgactggttc tcagtgaccg ccagctcccc gttcatatca tccctgaact caacgaaatg
    25681 ttttttggcg actgggagat gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac
    25741 tatagcgcgt ggtgcaatga ctggcagcat gctatcccca cgaacggtga aggatttcag
    25801 gcattttcgc aacgtgtgga acgctttatc gcaaggctta gtgaatatca gcactatcag
    25861 aatattttaa tcgtcagcca tcagggcgta ctgagtctgt taattgctcg tttaattggc
    25921 atgcctgccg agtctatgtg gcattttcgc gttgaccagg ggtgttggag cgccattgat
    25981 atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaac
    26041 gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacagc cccgggcagg
    26101 ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa attgttttac
    26161 aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat
    26221 tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc
    26281 tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga
    26341 caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcaag gcaaatccag
    26401 catcaccgac tgcatgatca tctgtacggg tacgtccagc cgtcatgtta tgtccattgc
    26461 tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga
    26521 aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga
    26581 agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact
    26641 tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg
    26701 tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggca aacgcggcaa
    26761 gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg
    26821 caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc cgcagttagc
    26881 cgctgagctg gaacgctgga agctggatgg tcgcgacgta agtctactga ttggcgggcc
    26941 tgaagggttg tcgcctgcct gtaaagcggc ggctgagcag agctggtcgc tgtcggcgct
    27001 taccctcccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag
    27061 catcaccacc aaccatcctt atcaccgtga gtgataaggg agctttgagt agaaaacgca
    27121 gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg
    27181 ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct
    27241 gtataatctg caaattgttc gctttaccga ctaccagacc cgctctaatg aaaaccgcat
    27301 taagctggtg cctatcgcgc ccagccgcgg cattatctac gaccgtaacg gtatccctct
    27361 ggccctcaac cgcactatct accagataga aatgatgccg gagaaagtcg ataacgtgca
    27421 gcaaacgctg gacgctttgc gcagcgtggt agatctgacc gatgacgata ttgctgcatt
    27481 ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa ctaacctaac
    27541 cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa
    27601 aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca tcggctatgt
    27661 gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta
    27721 tgcggcaacg catgatattg gtaagctggg cattgagcgt tactatgaag acgtgctgca
    27781 cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt
    27841 aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc tcaaactcca
    27901 gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggttgtta ccgaaccgcg
    27961 tacaggtggg gtgctggcgc tggtttccac gcctagttat gacccaaacc tgtttgttga
    28021 cggtatctcc agcaaagatt attccgcctt gctgaacgac ccgaatacac cgctggtgaa
    28081 ccgcgccaca cagggggttt atcctcccgc gtctacagtt aaaccctatg tggcggtttc
    28141 ggcattaagc gccggggtga tcacgcgcaa tacgacgctg tttgacccag gctggtggca
    28201 actaccaggg tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa
    28261 tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat
    28321 ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt acaccggtat
    28381 cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac agaaacgctt
    28441 taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggccagg gttactggac
    28501 agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa
    28561 ggttccgcat ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacaacc
    28621 gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga aagacggtat
    28681 gtacggtgtt gctaaccgcc ctaatggtac ggcgaataaa tactttgcca gcgcaccgta
    28741 caaaattgcg gcgaaatctg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta
    28801 taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc
    28861 atacaacaat ccgcaagtgg ctgtcgccat gattctggag aacggcggcg cgggtccggc
    28921 ggttggtacg ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga
    28981 tctgcctgcg gaaaatccag cggttgccgc agcggaggac cattaatcat gacggataat
    29041 ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatttta
    29101 ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc
    29161 atgatggagc gtaaaatcgg ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg
    29221 caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt
    29281 ttgctggtgg cggtagatgc tttcggagcc atctctaaag gcgctcaacg ctggctggac
    29341 ctcggtattg ttcgttttca gccgtcggaa attgccaaaa tagccgtacc actgatggtt
    29401 gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga agaatactgg catcgcgctg
    29461 gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacctggg aacatcaatc
    29521 ctcgttgcgc tttccggtct gtttgtactg ttcctctctg gccttagctg gcgtctgatt
    29581 ggcgtcgcag tagtgctggt agcggcgttc attccgattc tgtggttctt cctgatgcat
    29641 gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgcg
    29701 ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc
    29761 tggctgcacg gcactcagtc acagcttgaa tttctccccg aacgccatac tgactttatc
    29821 ttcgcggtac tggcggaaga gctgggatta gtgggcattc tgattctgct cgctctctac
    29881 attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc
    29941 gtcatggctg gtggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg
    30001 gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg
    30061 gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa
    30121 atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat
    30181 cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga cggtaagtgt
    30241 accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcgg acccgcgttt
    30301 cgaaccactg aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa gctacaaaat
    30361 cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg atgccgaacc
    30421 aggcagtaac ctgacggcct ctggcgaagc tttcgatccg acaaagctga cggcggccca
    30481 tccaacgctt ccgatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat
    30541 cgtggtgcgc attaatgatc gcggccctta cggcaacgac cgcgttattt cgctttctcg
    30601 cgcggcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat
    30661 tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca
    30721 gacttacgcc ctgcctgcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc
    30781 tggcccgcag ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac
    30841 cggcgcgccg gtaaccagta gcggtttcct cggcgctcca acgaccttag cgcctggtgt
    30901 actggaaggc agcgaaccga cgcctgcgcc acagcccgtt gttacagctt cgtcgacaac
    30961 gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa
    31021 ctttatggtg caagtcgggg ccgtaagcga tcaggctcgt gcgcaacagt accaacagca
    31081 actgggacag aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg tctggcggat
    31141 ccagcttggc ccattcgcca gcaaagccga agccagtacc ttgcagcaac gtttgcaaac
    31201 cgaagcccaa ttacagtcat ttattactac cgcgcagtag cgtaaagcag gcatctgatg
    31261 tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag
    31321 tgcatttgct atagtagggc acttttttaa ttccatcacg gatgtcgtag ttcagaccat
    31381 gaataccatt ttttccgctc gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac
    31441 agcctttatc tctgctgcac atgccgatga cctgaatatc aaaactatga tcccgggtgt
    31501 accgcagatc gatgcggagt cctacatcct gattgactat aactccggca aagtgctcgc
    31561 cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg accaaaatga tgaccagtta
    31621 cgttatcggc caggcaatga aagccggtaa atttaaagaa actgatttag tcactatcgg
    31681 caacgacgca tgggccaccg gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa
    31741 accgggcatg caggttccgg tttctcagct gatccgcggt attaacctgc aatcgggtaa
    31801 cgatgcttgt gtcgccatgg ccgattttgc cgctggtagc caggacgctt ttgttggctt
    31861 gatgaacagc tacgttaacg cactgggcct gaaaaatacc cacttccaga cggtacatgg
    31921 tctggatgct gatggtcagt acagctccgc gcgagatatg gcgctgatcg gccaggcatt
    31981 gatccgtgac gtaccgaatg aatactcgat ctataaagaa aaagaattta cgtttaacgg
    32041 tattcgccag ctgaaccgta acggcctgtt atgggataac agcctgaatg tcgacggcat
    32101 caaaaccgga cacactgaca aagcaggtta caatcttgtt gcttctgcga ctgaaggcca
    32161 gatgcgcttg atttctgcgg tgatgggtgg acgtactttt aaaggccgtg aagccgaaag
    32221 taaaaaactg ctaacctggg gcttccgttt cttcgaaacc gttaacccac tgaaagtagg
    32281 taaagagttc gcctctgaac cggtttggtt tagtgattct gatcgcgctt cgttaggggt
    32341 tgataaagac gtgtacctga ccattccgcg tggtcgcatg aaagatctga aagccagcta
    32401 tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat
    32461 caacttccag cttgatggca aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat
    32521 cccggaaggt aacttcttcg gcaaaatcat tgattacatt aaattaatgt tccatcactg
    32581 gtttggttaa aaattgaaca cttgaaagtg taatttccgt ccccatatac taagcatcag
    32641 taaaaaaact cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac
    32701 atgaaaacca aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg
    32761 gggcaggcgt tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca
    32821 ggtgactaca ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc
    32881 actatcaacg ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc
    32941 gatattgtcc gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac
    33001 tcccctctcc tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc
    33061 cgttttgtat caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat
    33121 ctcccgggct atgcatgaat tcaccgatac ccgcgataat agtacccttg atgaaatctg
    33181 gctggtcgag cactatccgg tattcaccca aggtcaggca ggaaaagcgg agcacatttt
    33241 aatgccgggt gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg
    33301 gccgggacaa caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg
    33361 tgaactggtg accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga
    33421 agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact
    33481 gggtttacga attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga
    33541 tctttcacca tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat
    33601 atcacaatgg aaacccgaag cgacgactaa taatattgct ccacgtttac tggaaaatat
    33661 tttagcgcta ctaaacaatc cggacttcga atatattacc gcttaattcc atacatcaat
    33721 ggcccaattt acactgggtc attactcata atacctgcta atgctttaca tctccttcgc
    33781 gtttgtatat tttctttatc acttgcagaa atgcggtgcc acgccttgct ccgtaactca
    33841 ccccattgat taacctattg aaatagttga ttatggtgag caaaagcgtt ccgttccaat
    33901 tagacataag attattgtaa ttttttaaat attatttttc catgaataaa attggagtaa
    33961 gtgcgtggat agtaataatc aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa
    34021 accacaaata tttacaacct tacgaaatat tgatcttaac cttctgacta tttttgaagc
    34081 tgtatatgta cataaaggga tcgttaatgc agcgaaagtg cttaatctga ccccctcggc
    34141 aatcagtcag tctattcaga aactgcgcgt tatattccct gacccattgt ttattcgcaa
    34201 aggccagggt gtcactccta ccgcatttgc gatgcatcta catgagtata tcagtcaggg
    34261 ccttgagtcc attcttggcg cgctggatat cgaaggaagc tatgataagc aacgaacgat
    34321 aactattgcg accactccct cggtcggagc cctggtcctt cctgtcatct atcgggcgat
    34381 taaaactcac tatccgcagc ttttactgcg caacccgccc atcagcgacg cggaaaacca
    34441 actcagtcag tttcaaaccg atctcatcat cgataacatg ttttgcacca atcgtacggt
    34501 gcaacatcat gttctgttca ccgacaatat ggtgttaatt tgccgcaagg gaaatccact
    34561 actctcttta gaagatgaca gagagactat cgacaacgct gcgcatgtac ttctgttacc
    34621 ggaagggcaa aatttcagcg gtctgcggca gagagttcaa gagatgtttc cggaccggca
    34681 aatcaatttc accagctaca acattttgac aatcgctgca ctggttgcca acagtgacat
    34741 gttagcgatt attcccagcc gtttttataa cctgtttagt cgttgctggc cgctggaaaa
    34801 actgcctttt ccgtccttaa atgaggagca aatagatttc tctatccact acaacaaatt
    34861 catcctgcgt gatcccatcc tgcacggagt aatagatgtt atccgtaatg cattttgaaa
    34921 aagtattgtt acagccagat cacagcgcag aacgataaaa tggcacaatc caacaacaat
    34981 tttacatttt cgcgaccgct ttggctgctt tcaggtccgt ttcaatgata tactgccagt
    35041 cgttaattca aaaatagttg ataattacaa caatctattg aattgaaacg ctttccttcg
    35101 taattcgcaa ctggaacacg cacgctatga gtaaacccat tgtgatggaa cgcggtgtta
    35161 aataccgcga tgccgataag atggccctta tcccggttaa aaacgtggca acagagcgcg
    35221 aagccctgct gcgcaagccg gaatggatga aaatcaagct tccagcggac tctacacgta
    35281 tccagggcat caaagccgca atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct
    35341 cctgccctaa cctggcggaa tgcttcaacc acggcacagc aacgtttatg atcctcggcg
    35401 ctatttgtac ccgccgttgt ccgttctgtg atgttgccca cggtcgcccg gtagctcctg
    35461 atgccaatga accagtgaaa ctggcgcaga ccattgccga tatggcgctg cgttatgtgg
    35521 ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg tgcccaacac tttgcggatt
    35581 gtattactgc cattcgggaa aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt
    35641 tccgcggtcg aatggatcgt gctctggata ttctgactgc aacgccccca gatgtgttca
    35701 accataacct ggaaaacgta ccgcgtattt accggcaggt acggcctggc gcagattaca
    35761 actggtcgct gaagctgctg gaacgcttta aagaagcgca tccggaaatc ccgaccaagt
    35821 ctggtctgat ggtgggtctg ggtgaaacca atgaagaaat tattgaggta atgcgcgacc
    35881 tgcgccgtca tggtgtgacg atgttaacgc tggggcaata tttgcagcca agccgccatc
    35941 acctgccggt tcaacgttac gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc
    36001 tggcgatggg ctttacccat gctgcatgcg gtccgtttgt ccgctcttct taccacgccg
    36061 atttgcaggc gaaaggaatg gaagttaagt aatcctgtaa taattactta catctgtctg
    36121 ataaaaaacc cgctttttga agcgggtttt ttgtatcgaa caagatattg agggagcgtc
    36181 ctgctcgcca cgtcactctt tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc
    36241 ttttttcgcc gcagcatcgt catcattcat cgccttcttg aaccctttaa tggccgctcc
    36301 aaggtctccg cccagcgtac gtaacttctt agtcccaaac agcagaacga ccagcgccgc
    36361 aactaccagc agtttggtaa tactaatctc acccatagat accttcttga catataaccg
    36421 gcaacagaac gcgccttaaa aaccagagaa tattaacggt cattcgacgc gcgcacacaa
    36481 tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa aacatcataa taattgcggc
    36541 ggcgcaaagc gacggttgtt taagatgggc agttgcgcgc gcacctgacg cacacgttcg
    36601 ggcgtcactt ccgccataat gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca
    36661 aagggatcta taatccggct ttgcccgata tttttgttcc cgcactcccc cgccgccacc
    36721 atataacagg tggtatccag cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg
    36781 agcggcccac gaacccaggc ggcaggaagt accaggattt cagctccctg taaagcctgt
    36841 gccagcgcca gctctggaaa gcgtaagtca taacaggtca tcagaccgac cttcatccct
    36901 tccacctcca gtaacggagc tatttcatta ccagcatcaa catggcgtga ttcctgaatg
    36961 gcaaatgcat catagagatg cagtttggca taacgggcga cgatgtttcc tgcctgaagt
    37021 gccaccagca tattccatgc gcgccccggc gttgaaggaa gatgaatcgt cagaattgtc
    37081 gtcatcatgt tacgtttact ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc
    37141 tgtgctgatt taaccgatag atctgcatca tgatcatcgc gcgccagcaa tgcttccggc
    37201 aggacaaaca gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca
    37261 gcgttctttt cccacacaga tgtaacagca aactgtcctg cagcaaccag cattgccacc
    37321 tcctgtcaga gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat
    37381 acaaatcaaa tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg
    37441 tggtacggga agcgtagcga gatggctgtt aagtatgcga tttaacccgc tgcatcaggc
    37501 gattccgttg gggacgctgg cagcaaacct gattggggca ttcatcatag gaatgggatt
    37561 cgcctggttc agcaggatga cgaacattga tccagtgtgg aaagtattaa tcaccaccgg
    37621 attttgtggc ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca
    37681 agagggccgc tttggctggg cattactgaa cgttttcgtc aaccttctgg ggtcttttgc
    37741 catgaccgca ctggcattct ggctgttttc ggcctcaacc gtacactaaa gaccaaaaaa
    37801 aacccgctga ttaagcgggt tttgaattct tgctgacgta tcttacagag cgattacgtt
    37861 tgcagcagaa gggcctttgg caccgttagt gatttcgaac tctacgcgct gaccttcagc
    37921 aagagtttta aaaccattag tctggattgc agagaagtgt acgaacacgt ctttgctgcc
    37981 gtcttccgga gtaatgaaac cgaatccttt ggactcatta aaccacttaa cgttaccttt
    38041 aatcttagac atcaaaatta cctttacatg aaaaatagac acaaatgctg tgtcggttat
    38101 cagtacacca attgtggtac gctttgtcca gtgcaacttt actaaaaagt gataaaagtc
    38161 gcgttatttt tgatgttgct tattttagtt attgatttta aagaagttac taaaacttca
    38221 tttgtctcaa aactgaaagc gcatccaggc aaagtacaca ttgccattgt tgtaggtacc
    38281 cggaatgtag gtcatctgaa aagtcactgg gccataaccc acggaggcca atggcagtag
    38341 aaccgggaga gggatgtaat tccagttatc gcgtgccgtt acgccagcgg tgaatcccag
    38401 acctaaatga aaattttcat ccgccagcgg tcgccaggta ctttcccatc cgtatccggc
    38461 aatcggttcc catttgttcc acgagtcctt aaatgccatg gcatacaggc catgccagtt
    38521 tcctttttca tcccaacgcg acaggccaaa accgccaccc cacggtcgct cgttatagcg
    38581 atcggttttt tctttgtcgt aagcgaaacg tgcatgccag gtgatggcag gaatatataa
    38641 atcataatgt tcaggctgtt gccaggtttg tgcaatattt tctctaaacg ttgtcatcct
    38701 ctcatctgcg ttagcaaaaa ctttaccaac gctgattaac tgaataaaaa caaaggaaaa
    38761 gatagcgaca tatttactca cgttcatttg tgaccataaa acatttatca aaaatctact
    38821 actagcatag caaagctaca attaacataa ccttaataga cccaacataa agaataatct
    38881 gaatagctgc tctatttatt tccgcttgtt caagttaaat atttccgcgc atcgattaaa
    38941 gatgatgcgt cgttttctgc gatgggaata gtcaaaaaag aaaaaccaag tcttttttga
    39001 tgacaaaatg caatcaagga aaaattattt tattttttta acgagataca acaatcatct
    39061 taacgaagta tataatatat atcgtcaacc catgctcata ttagattatt ttttgagcgc
    39121 acagccgcag ctaacaccat tgcaattaac aaatttgcat caatccacca tcaatttaca
    39181 cacattatta tgtgataatt gccaaccgct aaatatgcgt tttgttatct atgtataaaa
    39241 acagcaactt caatgtctta atggcagttt ttcttgattt taatcagcat tcatcgccaa
    39301 tttattgggc atattttttc cttaagcttt aggaagtttt tattatttac tttgggcctg
    39361 gagacaggaa aaattatgct gacattcatt gaactcctta ttggggttgt ggttattgtg
    39421 ggtgtggctc gctacatcat taaagggtat tccgccactg gtgtgttatt tgtcggtggc
    39481 ctgttattgc tgattatcag tgccattatg gggcacaaag tgttaccgtc cagccaggct
    39541 tcaacaggct acagcgccac ggatatcgtt gaatacgtta aaatattact aatgagccgc
    39601 ggcggcgacc tcggcatgat gattatgatg ctgtgtggct ttgccgctta catgacccat
    39661 atcggcgcga atgatatggt ggtcaagctg gcgtcaaaac cattgcagta tattaactcc
    39721 ccttacctgc tgatgattgc cgcctatttt gttgcttgtc tgatgtcact ggccgtctct
    39781 tccgcaaccg gtctgggggt tttgctgatg gcaaccctgt ttccggtgat ggtaaacgtt
    39841 ggtatcagtc gtggcgcagc agctgccatt tgtgcctccc cggcggcgat tattctcgca
    39901 ccgacttcag gggatgtggt gctggcggcg caggcttccg aaatgtcgct gattgacttc
    39961 gccttcaaaa cgacgctgcc tatctcaatt gctgcaatta tcggcatggc gatcgcccac
    40021 ttcttctggc aacgttatct ggataaaaaa gagcacatct ctcatgaaat gttagatgtc
    40081 agtgaaatca ccaccactgc ccccgcgttt tatgccattt tgccgttcac gccgatcatc
    40141 ggtgtactga tttttgacgg taaatggggt ccgcaattac acatcatcac tattctggtg
    40201 atttgtatgc tgattgcctc cattctggag ttcctccgca gctttaatac ccagaaagtt
    40261 ttctctggtc tggaagtggc ttatcgcggg atggcagatg cgtttgctaa cgtggtgatg
    40321 ctgctggttg ccgctggggt attcgctcag gggcttagca ccatcggctt tattcaaagt
    40381 ctgatttcta tcgctacctc gtttggttcg gcgagtatca tcctgatgct ggtattggtg
    40441 attctgacca tgctggcggc agtcacgacc ggttcaggca atgcgccgtt ttatgcgttt
    40501 gttgagatga tcccgaaact ggcgcactct tccggcatta acccggcgta tttgactatc
    40561 ccgatgctgc aggcgtcaaa cctgggtcgt accctatcac ccgtttctgg cgtagtcgtt
    40621 gcggttgccg ggatggcgaa aatctcacca tttgaagtcg taaaacgcac ctcggtgccg
    40681 gtgcttgttg ggctagtgat tgttatcgtt gctacagagc tgatggtgcc aggaacggca
    40741 gcagcggtca caggcaagta aatagtaatg ccggaggcaa gtttcctccg gctttatcat
    40801 gtcacccact gtggtatatg cgttgtggtc tgccaacttt gccgtgaaca atttcagcaa
    40861 taatcagatg gcggctggcg caatattcaa gataacgcct ggcagtggtg cggctgatgg
    40921 tcagtgcctg cgccaccgtt tctgccgtat gttgcacacc aggctcttta aacagttttc
    40981 gcaccgcgtt tagcgtcaag gggtcaatgc cggtcggcag ttcgtctttg ggttcaccgc
    41041 gagcataggc attaaacatc tcatcaattt gcttctggct ggcgctatca atactttcca
    41101 gcatatgttt acgctggcgg aaacgggtta gcgtttgccc cagtgtattg attcacttga
    41161 agtacgaaaa aaccgggaga acattggatt attcgggatc tgatgggatt agatttggtg
    41221 gggcttgcaa gcctgtagta caaattttag gcgttaacca atgaaacgcg aaagatagta
    41281 aaaaattgct tttgtttcat tgaaaaaacg aaaaacaaaa acactataaa tcatttcaat
    41341 catagcttca aaaaacgttc aaaaccgata acaaccaggc tgtcaccaaa tgactcatat
    41401 cacaaatcag ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg
    41461 ttttagctgg ctcatggttg ttatacggct tttttcgcat cttgtggttc caacaaagga
    41521 gctacaggct cttcctgttt atgaacacca tacattgaca aaatctcttg gcgagtttct
    41581 ggaggtaatt tatgcagcgc caaagctgtt tgtagtgttt ggcgatcaat acccagcgtt
    41641 gccaataggg tgtcaacgga gatggagggg cgttcttgca ttaatccgga aagcccatat
    41701 ttagccatgt ttagctctat ctctcggact tcattttctc ctagtttatt gattaacagg
    41761 gagaacattt tcaatgtatc agcattcatt tcttcgatag caattttttg catagccggt
    41821 attgccggga tatattcaac cccaggcccg cgacgccctt cttgtgaacg cttcggccat
    41881 ccttctttct ttgctcgttt atgcaccccc tgtattgtcc caggcattcc atccagttca
    41941 gtaatttgtt tgggtgtcat ccactggttt atctcatttg cgcttttcat aaactaagtt
    42001 taccagattt atctatataa aaatcaatgg attacaaaat aaaccacatt aactgatata
    42061 aaccggtttg gtttatttgg taatatataa aacagataat caagacgaat tatttgtttt
    42121 ggtaaccctt tatcatacgc aatatggttg gtgtgcgaaa gatgagtaac gattggcatc
    42181 gggccgacgt gattgcggcc ctcaaaaaaa caggaacccc tctggcaaaa ttgtccagac
    42241 gttcaggtta ttcaaccggg acgcttgcaa atgcattagt caggccatgg cctaaaggtg
    42301 aacgcattat tgctgacgcc ttaaacatga aacctgagga aatttggcct agtagatatc
    42361 taagggatca caatgagaga gtggatatca gcaattgaat gcatgggttt atctggttta
    42421 cctggttcat taatgggggt tcacaaaaga gctcagagag aaggatggct caagagaaga
    42481 agagaaggaa ttagaggggt tgcttacgaa taccatatat cttctcttcc taacattgct
    42541 cgagctgaag ttttgctcca acaaggaaaa tttaaaacca gtcagggatg ttttgaaatc
    42601 gcccgcccca cgctggaagc tcatgattat gatcgtgagg cactatggag caaatgggat
    42661 aaagccagcg attcccagcg cagcctggct gaaaaatggt tgccctcaat tcaggccaca
    42721 gacgaaatgc tgaaccaggg gatttcaacg aaaacggctt ttgcgaccgt tgcagggcat
    42781 taccaggtca gcgcatccac tttgcgggac aagtattacc aggtacagaa gtttgcgaag
    42841 cctgactggg cggctgcact tgttgatgga cgtggagcat cccgtcgcaa tgttcacaaa
    42901 agtgaatttg acgaggatgc ctggcagttt ctgattgcag attatctgcg accggaaaaa
    42961 cccgccttcc gcaaatgtta tgagcgtctg gaactggcag cccgcgagca tggctggagt
    43021 attccctccc gtgccacggc ctttcgccgg attcagcaac tggacgaggc aatggttgtt
    43081 gcctgtcgtg aaggtgaaca tgcactgatg catctgatac cggcacagca gcgaactgtg
    43141 gaacacctgg acgccatgca gtggatcaac ggcgacggtt atctgcataa cgtctttgta
    43201 cgctggttta acggtgatgt gatccgtccg aaaacatggt tctggcagga tgtgaaaacc
    43261 cgaaaaattc tgggctggcg ctgcgatgtg agcgagaaca ttgattcaat tcgcctctcg
    43321 ttcatggatg ttgtgactcg ctacagtatc ccggaggatt ttcacatcac cattgataac
    43381 acccgtggtg ctgcgaataa atggctgacg ggaggcgcgc ccaatcgcta ccgctttaag
    43441 gtaaaagagg acgatccaaa aggactgttt ttactgatgg gtgcgaaaat gcactggaca
    43501 agcgttgttg ccggtaaagg ctggggccag gcaaaacctg ttgaacgtgc tttcggtgtt
    43561 ggtgggcttg aggaatacgt tgataagcat ccggcactgg ctggcgcata tacggggcca
    43621 aatccgcagg caaaacctga taactatggc gaccacgctg ttgatgcaga gctgtttctg
    43681 aaaacctttg ccgaaggtgt ggcgatgttc aatgccagaa caggccgtga aacagaaatg
    43741 tgcgggggca aactctcgtt tgatgatgtt ttcgagcgtg aatacgccag aacgattgtg
    43801 cgtaagccaa ccgaagaaca aaaacggatg ctgttactgc ctgccgaggc ggtgaacgtt
    43861 tcacgcaaag gcgagtttac gcttaaagtt ggcggctccc ttaaaggcgc gaaaaacgtt
    43921 tattacaaca tggcattaat gaatgccgga gtgaaaaaag ttgtggtcag atttgatcca
    43981 cagcagttac acagcacg
//
LOCUS       DAJTPG010000024        42594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTPG010000024 DAJTPG010000000
VERSION     DAJTPG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO230_001370"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO230_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5691192.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPYNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO230_001371"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO230_001371"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5691193.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO230_001372"
     CDS             complement(3736..4092)
                     /locus_tag="OO230_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5691194.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO230_001373"
     CDS             complement(4096..4512)
                     /locus_tag="OO230_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5691195.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO230_001374"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO230_001374"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5691196.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO230_001375"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO230_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO230_001376"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO230_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5691197.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO230_001377"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO230_001377"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5691198.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO230_001378"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO230_001378"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5691199.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO230_001379"
     CDS             10521..11504
                     /locus_tag="OO230_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5691200.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO230_001380"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO230_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5691201.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO230_001381"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO230_001381"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5691202.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO230_001382"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO230_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO230_001383"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO230_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5691203.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO230_001384"
     CDS             complement(15306..15515)
                     /locus_tag="OO230_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5691204.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO230_001385"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO230_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5691205.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO230_001386"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO230_001386"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5691206.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO230_001387"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO230_001387"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5691207.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO230_001388"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO230_001388"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5691208.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO230_001389"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO230_001389"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5691209.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO230_001390"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO230_001390"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5691210.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO230_001391"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO230_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO230_001392"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO230_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5691211.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO230_001393"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO230_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5691212.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO230_001394"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO230_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5691213.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO230_001395"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO230_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5691214.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO230_001396"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO230_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5691215.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO230_001397"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO230_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5691216.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OO230_001398"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OO230_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5691217.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO230_001399"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO230_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5691218.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OO230_001400"
     CDS             complement(34461..34604)
                     /locus_tag="OO230_001400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691219.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OO230_001401"
     CDS             34605..34883
                     /locus_tag="OO230_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691220.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OO230_001402"
     CDS             complement(34930..37026)
                     /locus_tag="OO230_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5691221.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OO230_001403"
     CDS             complement(37026..37763)
                     /locus_tag="OO230_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5691222.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OO230_001404"
     CDS             complement(37760..38398)
                     /locus_tag="OO230_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5691223.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OO230_001405"
     CDS             complement(38512..38754)
                     /locus_tag="OO230_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691224.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO230_001406"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO230_001406"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5691225.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..>42594
                     /gene="lysC"
                     /locus_tag="OO230_001407"
     CDS             41391..>42594
                     /gene="lysC"
                     /locus_tag="OO230_001407"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5691226.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACG"
BASE COUNT        10597 a        10972 c        10451 g        10574 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt atacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgt atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgataccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcg
//
LOCUS       DAJTPG010000025        42536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTPG010000025 DAJTPG010000000
VERSION     DAJTPG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..813)
                     /gene="ycjM"
                     /locus_tag="OO230_001408"
     CDS             complement(<1..813)
                     /gene="ycjM"
                     /locus_tag="OO230_001408"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5691227.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITE"
     gene            complement(1025..1372)
                     /gene="pspE"
                     /locus_tag="OO230_001409"
     CDS             complement(1025..1372)
                     /gene="pspE"
                     /locus_tag="OO230_001409"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5691228.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1415..1636)
                     /gene="pspD"
                     /locus_tag="OO230_001410"
     CDS             complement(1415..1636)
                     /gene="pspD"
                     /locus_tag="OO230_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5691229.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1645..2004)
                     /gene="pspC"
                     /locus_tag="OO230_001411"
     CDS             complement(1645..2004)
                     /gene="pspC"
                     /locus_tag="OO230_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5691230.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2004..2228)
                     /gene="pspB"
                     /locus_tag="OO230_001412"
     CDS             complement(2004..2228)
                     /gene="pspB"
                     /locus_tag="OO230_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5691231.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2282..2950)
                     /gene="pspA"
                     /locus_tag="OO230_001413"
     CDS             complement(2282..2950)
                     /gene="pspA"
                     /locus_tag="OO230_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5691232.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3117..4094
                     /gene="pspF"
                     /locus_tag="OO230_001414"
     CDS             3117..4094
                     /gene="pspF"
                     /locus_tag="OO230_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5691233.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4214..5479)
                     /gene="puuE"
                     /locus_tag="OO230_001415"
     CDS             complement(4214..5479)
                     /gene="puuE"
                     /locus_tag="OO230_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5691234.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5517..6797)
                     /gene="puuB"
                     /locus_tag="OO230_001416"
     CDS             complement(5517..6797)
                     /gene="puuB"
                     /locus_tag="OO230_001416"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5691235.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6799..8286)
                     /gene="puuC"
                     /locus_tag="OO230_001417"
     CDS             complement(6799..8286)
                     /gene="puuC"
                     /locus_tag="OO230_001417"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5691236.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8436..8993)
                     /gene="puuR"
                     /locus_tag="OO230_001418"
     CDS             complement(8436..8993)
                     /gene="puuR"
                     /locus_tag="OO230_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5691237.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9020..9784)
                     /gene="puuD"
                     /locus_tag="OO230_001419"
     CDS             complement(9020..9784)
                     /gene="puuD"
                     /locus_tag="OO230_001419"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5691238.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9996..11414
                     /gene="puuA"
                     /locus_tag="OO230_001420"
     CDS             9996..11414
                     /gene="puuA"
                     /locus_tag="OO230_001420"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5691239.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11563..11727
                     /locus_tag="OO230_001421"
     CDS             11563..11727
                     /locus_tag="OO230_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691240.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11717..13102
                     /gene="puuP"
                     /locus_tag="OO230_001422"
     CDS             11717..13102
                     /gene="puuP"
                     /locus_tag="OO230_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5691241.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13236..13481
                     /locus_tag="OO230_001423"
     CDS             13236..13481
                     /locus_tag="OO230_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5691242.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13794..15437
                     /gene="sapA"
                     /locus_tag="OO230_001424"
     CDS             13794..15437
                     /gene="sapA"
                     /locus_tag="OO230_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5691243.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15434..16399
                     /gene="sapB"
                     /locus_tag="OO230_001425"
     CDS             15434..16399
                     /gene="sapB"
                     /locus_tag="OO230_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5691244.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16386..17276
                     /gene="sapC"
                     /locus_tag="OO230_001426"
     CDS             16386..17276
                     /gene="sapC"
                     /locus_tag="OO230_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5691245.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17276..18268
                     /gene="sapD"
                     /locus_tag="OO230_001427"
     CDS             17276..18268
                     /gene="sapD"
                     /locus_tag="OO230_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5691246.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18270..19076
                     /gene="sapF"
                     /locus_tag="OO230_001428"
     CDS             18270..19076
                     /gene="sapF"
                     /locus_tag="OO230_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5691247.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19144..19497
                     /locus_tag="OO230_001429"
     CDS             19144..19497
                     /locus_tag="OO230_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5691248.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19865..20653
                     /gene="fabI"
                     /locus_tag="OO230_001430"
     CDS             19865..20653
                     /gene="fabI"
                     /locus_tag="OO230_001430"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5691249.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20797..21924
                     /locus_tag="OO230_001431"
     CDS             20797..21924
                     /locus_tag="OO230_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5691250.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21992..23926
                     /gene="rnb"
                     /locus_tag="OO230_001432"
     CDS             21992..23926
                     /gene="rnb"
                     /locus_tag="OO230_001432"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5691251.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24004..24107)
                     /locus_tag="OO230_001433"
                     /pseudo
     CDS             complement(24004..24107)
                     /locus_tag="OO230_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24161..26135
                     /gene="pdeR"
                     /locus_tag="OO230_001434"
                     /pseudo
     CDS             24161..26135
                     /gene="pdeR"
                     /locus_tag="OO230_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26283..26456
                     /locus_tag="OO230_001435"
     CDS             26283..26456
                     /locus_tag="OO230_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691252.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26546..27295
                     /locus_tag="OO230_001436"
     CDS             26546..27295
                     /locus_tag="OO230_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5691253.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27564..27782
                     /gene="osmB"
                     /locus_tag="OO230_001437"
     CDS             27564..27782
                     /gene="osmB"
                     /locus_tag="OO230_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5691254.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(27908..28234)
                     /gene="yciH"
                     /locus_tag="OO230_001438"
     CDS             complement(27908..28234)
                     /gene="yciH"
                     /locus_tag="OO230_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5691255.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28234..28971)
                     /gene="pyrF"
                     /locus_tag="OO230_001439"
     CDS             complement(28234..28971)
                     /gene="pyrF"
                     /locus_tag="OO230_001439"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5691256.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29164..30333)
                     /gene="lapB"
                     /locus_tag="OO230_001440"
     CDS             complement(29164..30333)
                     /gene="lapB"
                     /locus_tag="OO230_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5691257.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30340..30648)
                     /gene="lapA"
                     /locus_tag="OO230_001441"
     CDS             complement(30340..30648)
                     /gene="lapA"
                     /locus_tag="OO230_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5691258.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(30797..31561)
                     /gene="pgpB"
                     /locus_tag="OO230_001442"
     CDS             complement(30797..31561)
                     /gene="pgpB"
                     /locus_tag="OO230_001442"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5691259.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            31731..32321
                     /gene="ribA"
                     /locus_tag="OO230_001443"
     CDS             31731..32321
                     /gene="ribA"
                     /locus_tag="OO230_001443"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5691260.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32385..35060)
                     /gene="acnA"
                     /locus_tag="OO230_001444"
     CDS             complement(32385..35060)
                     /gene="acnA"
                     /locus_tag="OO230_001444"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5691261.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35224..35319)
                     /locus_tag="OO230_001445"
     CDS             complement(35224..35319)
                     /locus_tag="OO230_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691262.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35433..35600)
                     /locus_tag="OO230_001446"
     CDS             complement(35433..35600)
                     /locus_tag="OO230_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691263.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35603..35767)
                     /locus_tag="OO230_001447"
     CDS             complement(35603..35767)
                     /locus_tag="OO230_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5691264.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36062..37036)
                     /gene="cysB"
                     /locus_tag="OO230_001448"
     CDS             complement(36062..37036)
                     /gene="cysB"
                     /locus_tag="OO230_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5691265.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37246..39843)
                     /gene="topA"
                     /locus_tag="OO230_001449"
     CDS             complement(37246..39843)
                     /gene="topA"
                     /locus_tag="OO230_001449"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5691266.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40223..40474
                     /locus_tag="OO230_001450"
     CDS             40223..40474
                     /locus_tag="OO230_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5691267.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40510..41559)
                     /gene="sohB"
                     /locus_tag="OO230_001451"
     CDS             complement(40510..41559)
                     /gene="sohB"
                     /locus_tag="OO230_001451"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5691268.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            41779..>42536
                     /locus_tag="OO230_001452"
     CDS             41779..>42536
                     /locus_tag="OO230_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5691269.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
BASE COUNT        10136 a        11080 c        10574 g        10746 t
ORIGIN      
        1 ttcggtaata atcactgtac ctggtgctac gtcatcaata atcgaccgta acagcttgat
       61 aatcagatgt gttttttcca gatggatgca gcttgttccc ggctctttcc acataaagcc
      121 aacggcatcc aggcggacat actcagcacc tttctcaagg taacagagta aaacatccac
      181 catcgccagc aacacttcag ggctacggta attcaggtca atttgatcgt cactaaaggt
      241 ggtccataaa tggcgcgtgg aattatcatc catttgaaac ggcgttaata gcggtaacgc
      301 acgcggaagg gtgacggcac tgagatcggt tttcggatca accgcaataa agaaatcttc
      361 aaaacccgga agctgttgta aatagttttt aaaccattca ctttttgcag acatatggtt
      421 gcagacaaaa tcaaacatta aatgactgca ttcaccgagt tgctgaatat cctgccactc
      481 ccccgcttca ctggcgacct gatgataatc aattaccgaa aatccatcat cagatgacca
      541 tgggtaaaaa ggtaataaat gaacatgtga aaaaatgctt tgcagccatt gatggtagaa
      601 ctgattaaat atgggtagtg gttttaaatc attactgtga aattgatcgg cataggtaat
      661 taacacgaca tcactttcat cccagtgttt tttacgtcgc tgtgtaatta atcgtttcgc
      721 actctcaaga cgcgttacaa gtttctgtaa atgagttgcg gtaaatgttc caccgtagat
      781 ttcgtccatg taatccgtaa ttttctgttt catgttgcct ttccgtggta gcggtcccat
      841 gaggcgagag actactccgc agaaaataaa ctgtcaattg agcacaaggg ttttagtcac
      901 gcaggaaatc attttttacc agggaaaaag cgtatttgtg agctgacgca aataatctat
      961 agggatgtaa ataaggtgtg acagaaaaaa aacggcgcat aagcgccgct catggtgaat
     1021 tcttttaacc tttgaccttc ggcattgcga tgtctttcag gccaccggcg ttctcaacgt
     1081 gggtatatcc catctcgcta aggagctctt ttgcctgccc ggactggcgt ccggcattgc
     1141 aatacacttt cacggtgtcg tttttatccg gaacggcggt ggcaatgcgc tctttcactt
     1201 ccttcagggg aatattgatg gccccctgaa cgtgctcttg ctgatactgc tctggaacac
     1261 gaacatcgat ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt gccagagcaa
     1321 gtaagccttt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata
     1381 cgcctgagat ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt
     1441 tattagcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc
     1501 ggcgagcaac tgattttatc gcccagcccg ccacacccgc cgggccatag cgcagtgcgg
     1561 taagaagtac cagcttgcct gctaatttga aaccaagctt caccttttgc ccggcctgtt
     1621 gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt
     1681 atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg
     1741 gtcgacttca tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc
     1801 cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag
     1861 accgaaaaaa atcgacagca ccaccaggat acgcaccagt tttaccggta catcaaaata
     1921 gttggcaatc ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataactt
     1981 tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga
     2041 atagattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcaac cagttgcgct
     2101 aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc
     2161 cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc
     2221 gcgctcataa gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt
     2281 attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat
     2341 catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc
     2401 tttctgcttc cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg
     2461 cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct
     2521 gatgacgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct
     2581 cgccaatctc tttcttcatg cgtgccagcg tatcgtccac cagcgtcact tcatgttcca
     2641 gggacttaat cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct
     2701 ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg
     2761 cttgttcaat acggcgagtc agctgtttct tttctgccaa cgcacgcgcc gaagtagaac
     2821 gtacttcaac cagtgtatct tccatctcct ggatcatcag acgcaccagt ttctgtggat
     2881 cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa
     2941 aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta
     3001 atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca
     3061 acagcaacat gccaggatga attagcgaat tacactaaca agtggcgaat ttcatcatgg
     3121 ccgaatacaa agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg
     3181 tttcgcatct cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta
     3241 aagagttgat tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt
     3301 cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag
     3361 cgggggcgtt taccggtgcg caaaaacgtc atcctgggag atttgaacgt gccgacggcg
     3421 gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggaa aaattattgc
     3481 gtgtgattga gtacggtgaa ctggagcgcg ttggcggtag ccagccgttg caggtgaatg
     3541 tgcggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggtacttttc
     3601 gcgctgacct gctcgaccgg ctggctttcg atgtagtgca actgccgccc ctgcgcgagc
     3661 gcgaaagcga cattatgctg atggcagaac actttgccat tcagatgtgt cgggaaatca
     3721 agctgcctct gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt
     3781 ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca
     3841 ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt ccacctgaag
     3901 aagccatcgc cgtttcagaa aatacctcgc ttccgacgct gccgctggat ttacgcgagt
     3961 ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacagggg aaatataacc
     4021 agaagcgcgc ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa
     4081 agcaccagat ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat
     4141 tcggcataca taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta
     4201 ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt
     4261 gcgcatccgg gatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg
     4321 tcagcaggag cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg
     4381 acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta
     4441 ccgcagcaat ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc
     4501 ccagttgatt cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc accgcgtgcg
     4561 cggcagccac cgccagcggg ttcccggcgt aggtgccacc caacccgccc ggcgcgggcg
     4621 cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc gcgaggcttt
     4681 tcgccatcgt cattaagtcc ggcttatcgg cgtaatgatc catggcaaac agtttaccgg
     4741 tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aataccgtgc tcgtcgcaca
     4801 ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgcac tcgccctgca
     4861 ccggttcgaa aataatggcc gcgacctgct tcgcttcgat gtctgattta aacaagcgtt
     4921 cgatggcgtc gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga taaggtacgt
     4981 gatacaccga accagggaac gggccgaagc cgattttgta cggcgcaact tttccggtca
     5041 gcgccatggt catatatgta cgaccgtgga agccaccgct aaacgcaatc acgccaggtc
     5101 gtccggtatg ggcgcgagca attttcaccg cgttttccac cgcttccgcg ccggtagtga
     5161 agaatgcggt tttagcctgc ccgctcactg gggcaagggt gttaattttc tccgccagcg
     5221 tgacgtagct ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt tgcaattgct
     5281 gctccaccgc cgcgaccaga tcagggtggc gatgcccggt attcagcacc gcaatgcctg
     5341 cggcgaaatc gatgtattcg ttgccctcta cgtccttcag cgtagcgttt tctgccgact
     5401 gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga cgacgctgat
     5461 ggaattcatt gttgctcatg tttcacacct tttgactctt cactgttaat cggaaattaa
     5521 acgcccagac gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa tggcacgcgc
     5581 agcgtgcgcc cgccggggaa cgggtaatgc ggcagattgg cgaaggcatc gaaacgctcg
     5641 gcgtcaccgc gcagcagttc agcaatcaaa cgcccggcaa gatgggtaca ggtgacgccg
     5701 tggccgctgt aaccctgcat gtaatagatg ttggtatcaa ggcggccgaa ctgcggcatg
     5761 tgcgacaagg tcagcaggaa gttgcccgtc cagcggtaat caattttcac gcccttcaac
     5821 tgagggaagg tttttagtag tttcggtacc acaagacgct caacgtcatc cgggttgcgt
     5881 gcaccataca ctacaccccc gccgtacagc aggcggttgt cggcggtaag acggtagtaa
     5941 tcaagcagat agttacagtc ttccacacag tagtttttcg ggatcagcga acgggctaaa
     6001 tcttccgaca gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct gcgtttcgct
     6061 agttccggct ctactttatc gcccagatac gcattcccgg cgacaatcac atacttcgcc
     6121 gtcacctgac cattggcagt ccgaaccacg gctggcgtgg tgtgctggat ctgcgtcacg
     6181 gcggaaagct catacacgcg cccgccgttc aggcggatgg catcggcttc accaatggcg
     6241 aggtttagcg ggtgaatatg cccaccgctg tgatccagca gcgcaccagt gtagcgatcg
     6301 ctggcgacct cgcggcgaat agcgttgacg tccagcagct ccagctgttt attgccgtag
     6361 cgtacccagt tctctttctg ctcttcgagt gtggcgagtt gtttatcatt catcgccaca
     6421 aacagaccgc cggggcggta gtcgcagtca atctgataac gtttgatacg ttcgcggatg
     6481 atctcgccac cctcgaacat catgctgccg agcatacggg cggtgtccat gccgtagctt
     6541 ttttcgatca cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc attgcgcccg
     6601 cttgcgccaa agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc cgcttccgcc
     6661 agatgcagcg cggaggagag cccggtatag ccgccgccaa ccacgcaaac gtcgcaggtg
     6721 attgactcat tcagtgtgtc gaatggtgca tatttattcg cactggcggc gtagtaactg
     6781 ctggtatgtt cggtcatttc aggcctccag gcttatccag atggttttca gttcagtgaa
     6841 tttttcaagg gcatgcaggg atttgtcgcg accgttgccg ctctgcttat agccgccaaa
     6901 cggcacggtc atatcgccgt cgttgtagtt attgacgaag acggaaccgg ctttcaggcg
     6961 tcggctcatg cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc caaggccgta
     7021 ctggctgtcg ttggcaagct gcagcgcctg atcttctgat gtgaaacggg tgaccaccag
     7081 caccggaccg aaaatctctt cgcggcttaa ggacgcattc gggtccacat ccacgaagat
     7141 ggtcgggccg atggccgctg ccagcccggc gttgcgacca tccagcagca gttgcccctt
     7201 gccttcgcct tcacgaataa aggtgtgtac cgagtcagcg tgagcgctgt cgattaaggt
     7261 gcccatggtg gttgcgggat caagtggatg gcccggctgc cagttttgcg cctgctgttt
     7321 taacagggct aagaattcat cggcgatgct ctcttccagt aacaaacgcg ttccggcgat
     7381 gcgcacctgg ccctggttgt agaagatgcc tgcggcggtg gcgcttgccg ctttttgcag
     7441 atccggacaa tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca gccagacacg
     7501 tttcatgttg ctatcacccg cgtctttcag cagttgttta cccgtacgcg tggagccggt
     7561 aaaggcgatg gcatcgatat cgttatgacg cgaaagcgcc tgccccgctt catgaccaaa
     7621 acccgtcacc acgttcaaca caccatccgg caagcctgct tctttcgcca gccccgcgag
     7681 acgaatcgca ctgagcggtg atttttcaga cggttttaga atcacgctgt ttcccgccgc
     7741 cagcgccggg ccgagtttcc agcaagtcag caacagcggg aagttccacg gcacgatggc
     7801 ggcaatcacg ccgaccggtt cacgcacgat catcgccagc tcatggctac tggtggtcgc
     7861 cacttcgcca tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc
     7921 gggaatatca tcacgcagac tgtgacgaat cggtttgccg gtgtcgagag tttccagcag
     7981 tgccagctct tcggcgtggg cttccattaa atcggcgagt ttattcagta ccgctttacg
     8041 tttagccgga gaagagagtg accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac
     8101 cgcacggtcg atatcgacgc tcttgccgcg ggcaattttc gccagcggtg cctgggtgac
     8161 cggatcaacg gtttcaaagg tttcattttc cgccgcagca gtatattcac cgttaataaa
     8221 taagcggttt tcaatggcga gacttaacgc tttatcctgc cagtaagcca gatgatgaaa
     8281 attcattatg actcctgttt cacgtctatc agatatatgc agtttgtgaa cgtcagggcg
     8341 cagagatccc cgggctgcat gcccgtttat ttctggcgac attctttatg gattgccggg
     8401 cgcattgcgc ttacccggct tacagaacaa aaagattaaa acgtggtggg cgtatgggcg
     8461 ctgataattc ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat gccggtatta
     8521 atggcataac tttgccccgc gacgaggtgg taatcctgac cattaatcgt cagaacaatt
     8581 tcaccttcca gtactgtgcc tatttcctca ccctgatgct taattctttc cccagtggtt
     8641 gtgcccggct ggtacgtttc aaagatcatc gccagcgtgc gattcgggtt accgttatga
     8701 accagcttca ttgacacacc ctgactgccc atctcaatta agtcgtcctg attaatgacg
     8761 acctgcggct catcaggttt ttccggctcg gaaaagaatt ccgagagtga cagaccatac
     8821 accttcagca gcttttgcag cgtactgatg gcagggctga ctttatcttg ttctatcgta
     8881 ctgatagcac tgtgagtcag cccggagagt tcggcggcac gacgttgtga aagcccctgc
     8941 tgctggcgga tttccgacaa gcgttttcct ggcgccagtc cctcatcact catatttgca
     9001 tttccttaaa ctgtagtggt cagagtcgct gtttttcagc gatatggtgc tgccaagcgg
     9061 tgataaagcc ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc cattccgggt
     9121 gccactgtac gcccagcgca aaaggatgat tgatgacgct aaccgcctca actaagccat
     9181 ccggcgagcg agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct tgtccatgta
     9241 gagagtttac ccaaaagttg ctacattcag gtaacaacgc agacagtaat cccccctctt
     9301 caacctgaac ttcatgcgac ggtgcatatt gctgttccac cggcagttcg ggatcttccc
     9361 gatgttccag caattcaggc tgttcgcaca gcttgcgatg caacgaacca ccggttgcca
     9421 ccaccagttc ttgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt tcgagtgcgg
     9481 cattaattat cgccatgctc agaagatcac gcccgggatc ggcgtcaggc tcatcgccgt
     9541 tttcaccata taggtgcggc tgcacattgc tgggactact aggaagataa atgccatcga
     9601 gtttcggcaa aagttgttca agtaacgacg gttccgccag cgcatgtggt agcgcaattg
     9661 gcaagccgcc tgcgtggatg atggcattca ggtacttttc ttgcagagtc tgggtcgcat
     9721 gacccttaag cctgttcctg cacattacga caccgataac cggattgttc attatatttt
     9781 ccatgctcaa tctcacaaaa tggactaaat tatcgccatt actgcgcttt aaatgacctt
     9841 tatgttcaat attttttcaa tctagcagtg gattttcatt tttgcaaact caatttaaca
     9901 tttgacaaac atttggtttg catacagatt cgaatgatgg tcattatatt ttacgctttg
     9961 ataacgagcg gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc gttgaagtag
    10021 agaactttgt tcagcagtca gaagagaggc ggggtagcgc ctttacgcag gaagtgaaac
    10081 gctacctgga gcgctacccg aatacgcaat atgttgatgt tttacttact gatttgaatg
    10141 gttgcttccg tggaaaacgt atccctgttt caagcctgaa gaagctcgag aaagggtgtt
    10201 atttcccggc ctcggtattt gcaatggata ttctgggcaa cgtagtagaa gaagcaggtc
    10261 tgggtcagga aatgggcgag ccggatcgta cctgtgttcc tgttctcggt tccttaactc
    10321 cttctgccgc cgatccagag tttatcggtc agatgctcct gaccatggtc gatgaagatg
    10381 gcgctccctt tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag cagctgcgcc
    10441 agcgcggatt gttcccggtt gtagcggtag agctggagtt ctatttactg gatcgccagc
    10501 gcgacgctga aggggatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    10561 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    10621 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    10681 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    10741 tggcactaaa acgcctagtg cgtctgatgg cagaaaagca taagatgcac gccactttta
    10801 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    10861 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    10921 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    10981 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    11041 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggtga ccgtcataat caccgcgtgg
    11101 aatatcacgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    11161 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    11221 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    11281 agaatgatca cctgcgccgc tatttaggcg aacggttttg ccatgtgtat cacgcctgta
    11341 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    11401 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    11461 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    11521 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    11581 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    11641 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    11701 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    11761 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    11821 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    11881 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    11941 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    12001 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    12061 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    12121 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    12181 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    12241 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    12301 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    12361 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    12421 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    12481 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    12541 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    12601 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    12661 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    12721 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    12781 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    12841 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    12901 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    12961 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    13021 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    13081 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    13141 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    13201 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    13261 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    13321 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    13381 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    13441 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    13501 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    13561 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    13621 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    13681 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    13741 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    13801 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    13861 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    13921 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    13981 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    14041 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    14101 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    14161 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    14221 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    14281 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    14341 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    14401 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    14461 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    14521 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    14581 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    14641 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    14701 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    14761 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    14821 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    14881 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    14941 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    15001 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    15061 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    15121 acccggacag tttcttccgt ccgttactga gctgcgcggc aattcattca cagaccaacc
    15181 tcgcccactg gtgcaatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    15241 agctggcagc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    15301 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    15361 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    15421 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    15481 gttcctgctg accttcgttg gcttcagctt gagctatttc actccacacg cgccgttaca
    15541 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaat ggcctgatcc actgggattt
    15601 tggtgtttcc agcattaacg gtcaaccaat tgccgaacag ttaaaagagc tcttcccggc
    15661 gacgatagaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    15721 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    15781 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacactgt tttgttcact
    15841 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    15901 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    15961 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    16021 tgaagtgatc cgcctgatgc gtatcagcac tattgaagtg tatgaccaga actatgtaaa
    16081 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    16141 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    16201 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    16261 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    16321 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    16381 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    16441 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcgggac
    16501 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    16561 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    16621 ggactgacga cctcggacgt gatgttttaa gccgtttact gagcggagct gcgccaaccg
    16681 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    16741 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    16801 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    16861 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    16921 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    16981 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    17041 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    17101 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    17161 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    17221 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    17281 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    17341 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    17401 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    17461 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    17521 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    17581 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    17641 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    17701 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    17761 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    17821 tgacgaaccg acaaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    17881 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    17941 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    18001 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    18061 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatactctac ccggcgctat
    18121 cccattgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    18181 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    18241 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    18301 taagtaaaac gttccgctac cggaccggtt ggtttcgtcg tcagaccgta gaagcggtaa
    18361 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    18421 cgggtaaatc cacactggca aaaatgctgg cgggaatgat agaacctacc agcggcgagt
    18481 tattgattga cgatcatccg ctgcattttg gcgattattc cttccgtagt cagcgtattc
    18541 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcacaaattc
    18601 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    18661 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    18721 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag
    18781 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc aatgcgttcg cagttgatta
    18841 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    18901 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    18961 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    19021 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    19081 ctgaatcctc cttccagatt attcacctca ttctgacaag agattaaatc ttgtcagaat
    19141 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    19201 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    19261 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    19321 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    19381 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    19441 gaggcggtgc tggagattat tctgcaggaa ctgaaccgcc ggtcaccctc accctgaaag
    19501 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    19561 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    19621 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    19681 gagagggtta gggtaagggg aaaagcgccc cccctgccgc agcctgctcc ggtcggacct
    19741 ggtaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    19801 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    19861 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc
    19921 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    19981 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    20041 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    20101 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    20161 gctggatggt gactatgtta acgccgttac ccgtgaaagc ttcaaaattg cccacgacat
    20221 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    20281 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    20341 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    20401 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcctccgg
    20461 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    20521 agttactatt gaagatgtgg gtaactctgc ggcattcctg tgctccgatc tctctgccgg
    20581 tatctccggt gaagtagtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    20641 cgaactgaaa taatcgttct gttggtaaag atgggcggcg ttctgccgcc cgttatctct
    20701 gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt
    20761 caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc
    20821 aaaagccact ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg
    20881 gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag
    20941 acgctggccc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc
    21001 cctgaccagc ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg
    21061 gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga
    21121 cttacgccgc tccccggacc tgattcctcc agggaaagta atcatcggtt ggcacaaatc
    21181 acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag
    21241 catctgaatg acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt
    21301 tttattggct ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta
    21361 cgctggttac ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa
    21421 tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt
    21481 tcaactgcag aactttgcca actggccgaa acactctctc tccaccctat ttcactttcc
    21541 cttctcgaaa agttgttaaa cagcacacgg gtcaatgcac agccggataa tcagcttgcg
    21601 gcgttgttat gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca
    21661 tcaaatgaat ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga
    21721 tgggctgacc gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga
    21781 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    21841 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    21901 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    21961 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    22021 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    22081 aaaaggcttt ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca
    22141 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    22201 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    22261 tcagggcaaa aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat
    22321 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    22381 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    22441 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    22501 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    22561 agatctgacc tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga
    22621 cgcccttttc gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga
    22681 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    22741 caccaactat ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct
    22801 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    22861 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    22921 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag
    22981 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    23041 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    23101 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    23161 agaagcgatg attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt
    23221 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    23281 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    23341 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    23401 cttccagtca tttgctgaaa ttagctctga acccggtcct cactttggcc tcggtctgga
    23461 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    23521 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    23581 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    23641 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    23701 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    23761 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    23821 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    23881 agtccgcatg gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    23941 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    24001 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    24061 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    24121 tttaaccgcg gactccgctg ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    24181 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    24241 cgatgctttg cgcttttcta ccaccaaaac cacagaacct gatcgtacat tgcagttatc
    24301 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    24361 tctgaccgtc gatgaaagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    24421 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcggttgctc gtgatttatc
    24481 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    24541 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca cagggttgaa
    24601 agaacacgac gtcattgggt aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    24661 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    24721 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaat ttgtccacag
    24781 cggcagtggc aaaaacgaga tttttttaat ctgttccggt accgacatta ccgaagagcg
    24841 ccgcactcag gagcgactgc gtattctggc aaataccgac agtatcaccg gactgccgaa
    24901 tcgtaacgca atgcaggagt taatcgatca cgctattaat caggcagata acaataaagt
    24961 tggggttgtg tatcttgatt tggataattt taaaaaggtc aacgacgcct atgggcattt
    25021 gtttggtgac cagttattac gcgacgtgtc attggctatt ttaagctgtc tcgaacatga
    25081 ccaggtgttg gcgcgtccag gtggcgatga gtttctggta ctggcatcca atacctcaca
    25141 aagcgcgctg gaagcaatgg catcacgaat tttgacccgc ttacgcctcc cctttcgtat
    25201 tggtttaatt gaagtttata ccagctgttc agtaggtatt tcgctctctc ccgaacatgg
    25261 ttcggacagc gcgactatta ttcgtcacgc cgacacagca atgtacacag cgaaggaagg
    25321 cggacgagga caattttgcg tttttactcc agaaatgaat cagcgggtat ttgaatatct
    25381 ctggctggat accaacttgc gtaaagcact ggaaaacgat cagttggtta tcgaaaatca
    25441 cctagcgtgt cgaagtgcgc agtctggaag cactagtacg ttggcagtca cctgaacgtg
    25501 ggttgattcc accgttggac ttcatttcct acgccgaaga gtcagggcta attgtgcctt
    25561 taggccgttg ggtgattctc gatgtcgtac gccaggtggc aaagtggcgg gataaaggca
    25621 taaacctgcg agtggcggta aatatttctg cacgtcagct cgccgatcaa accattttca
    25681 ccgccctgaa acaggttctc caggaactca attttgaata ctgccctata gatgttgaac
    25741 tgacagagag ttgtctgatt gagaatgatg aactggcact gtctgttatt caacaattta
    25801 gccaactagg tgcgcaagtg catctggatg attttggtac cggctactct tcactttcgc
    25861 aactggcgcg ctttccgatc gatgccatca aacttgacca ggtttttgtt cgagatattc
    25921 acaaacaacc tgtctcgcag tcactggtcc gggcgatcgt cgctgtggcc caggcattga
    25981 atcttcaggt gatcgccgaa ggtgtagaga gtgcaaagga agatgctttt ttaaccaaga
    26041 acgggatcaa tgagcggcaa ggatttttgt ttgccaaacc gatgcccgcc gtcgccttcg
    26101 aacgctggta taaacgctat ctgaagcgcg cataagtaga tgaatatata tacttagcgc
    26161 tatatcccgt cattatatat cgtattcctg gatgataatt agccgttgct gttaaacact
    26221 tctcttttag cggcaatttt tgcattattc attttccccc ttttgctata aggatgtcct
    26281 tgatgtctga atttgatgcc cagcgcgttg ccgaacgtat tgatattgtg ttagatatcc
    26341 ttgtggcagg cgattaccac tctgcgatcc ataatctcga gatacttaaa gcagaactct
    26401 tgcgccaggt tgctgaatct acgccagaca tcccgaagac accgtgggag atttaacaat
    26461 cgtgccttca ggttttccta tttgaggatg cccggcgcgt cagtattttt ttatttagta
    26521 ttataacgtt ataagaatta cagcgatgaa ttcccgacaa caaactattc tacagatggt
    26581 cattgaccag ggtcaggtta gcgtaaccga tctggcaaaa gccactggag tttctgaagt
    26641 taccattcgc caggatctca acaccctcga aaaactgagt tacctccgcc gtgcacatgg
    26701 ctttgcagtt tcgcttgata gtgatgacgt cgaaacccgt atgatgagca actatacgct
    26761 gaagcgtgaa ctcgccgagt ttgccgcgtc actggttcaa ccgggcgaaa ccatctttat
    26821 cgaaaatggc agcagcaatg ccctgctcgc ccggactctg ggggagcaga agaaaaatgt
    26881 cactatcatc acggtcagca gctacatcgc gcatttgctg aaagacgcgc cttgtgaagt
    26941 cattttgctc ggtggcgtgt accagaaaaa aagcgaaagt atggttggcc ctttgacacg
    27001 ccagtgcatc cagcaggtac atttcagcaa agcatttatt ggtattgatg gctggcaacc
    27061 tgaaactgga ttcaccggtc gcgatatgat gcgcaccgat gtggtcaatg ccgtgctgga
    27121 aaaagagtgc gaagcgatag tcctgactga cagctcgaaa tttggtgctg tacattcata
    27181 ctccatcggt cccgttgagc gattcaatcg cgtgattacc gattcgaaaa tacgcgccag
    27241 cgatctgatg catcttgagc acagtaaact caccgttcac gtcgttgaca tttaaccatt
    27301 tcccttctct acggatgatt tgcagtttgg caaatcatcc gctctaagat gattcctggt
    27361 tgataattaa gactatttac ctgttattaa cactctcaag atataaaatt attatcagcg
    27421 atataacagg aagtaattat cacctgcgtg atataaccct gcgcgcgagc agatttcacg
    27481 gaataatttc accaggctta ttcttagcta ttatagttat agagagctta cttccgtgaa
    27541 tcataaattc aggagagagt attatgtttg taacgagcaa aaaaatgacc gcggctgttc
    27601 tggcaattac tttggcaatg tctctgagtg cctgttctaa ctggtctaaa cgggaccgca
    27661 acaccgcaat cggcgcgggt gcaggggcat taggcggtgc agtactgacc gatggcagta
    27721 cgttgggtac attaggtggt gcagctgtcg gtggtgttat tggtcaccag gtcggtaaat
    27781 aatcgtaaat aaatgtcgat tatcgactcg caattataat acgcttgatt ctgttcagta
    27841 cgaaataact gaataataca cacagtaaaa tattcgaaag ccacggatat atccgtggct
    27901 ttttatgtta accgcctgcg agttttacct tcatcccttt cgcttcaagt agtgatttta
    27961 ataaatcacg cttatcgccc tggatttcaa taactccatc tttaactgct ccgccgcagc
    28021 cgcatttttt cttcagttcc gctgcaagtt ttgtcagttc ggcatcatcg agatcgatac
    28081 cggtaatcag gcaaacgccc ttacctttac gtccactggt ctgacgctga atacgcaccg
    28141 ctccatcgcc tttagggcgt actggggccg ctttgggctc atcaatacgc ccggtttccg
    28201 ttgagtagac cagacggctg ttggaatcac tcatcatgca ctccgctgta aagaggcgtt
    28261 gatcgctttc agtgtctgcg ctggatctac cgattgcgtt accgggcgac caatcaccat
    28321 ataatcaaca ccagccgcca acgcctgttc tggcgtcata atgcggcgct ggtcaccagc
    28381 ttcactcccc tgcggacgaa tgcccggtgt aaccagtttg aactcctgac cgaatacctg
    28441 tttaaagcgc acagcttcct gagcagaaca caccacacca tcaaggtcac atttttgcgt
    28501 cagtgctgcc agacgttctg cataatctgc aggtgacagt gtcacgccaa gatcggccag
    28561 gtcgctggct tccatgctgg tcaacactgt cacagcaatc aaaagcggtg catctttgcc
    28621 aaacggaacc agtgcctcac gcgctgcggt catcatacgc gccccaccgg aggcatgaac
    28681 attcaccatc cacacgccta agtcagctgc agcagcgaca gcgtgcgctg cagtgttggg
    28741 gatatcgtgg aatttcaggt caagaaagat atcaaaacca cgctgttgaa gttcgcgcac
    28801 aaactgtggc ccaaacaatg taaacatctc tttaccgacc ttcagacgac aatcgcgtgg
    28861 gtcgatcttg tcgacaaagg acagcgcgtc atcacaatta tgataatcaa gggcaacaac
    28921 cacaggagaa ttcgtaacgg cgcgggaaga agatgaagca gttaacgtca tgaccagacc
    28981 ttcttgatga tgggcaccgg gagtggcgcg aaacaggtaa acggcgagca ttctacccgt
    29041 gaaaataaaa aattaacagt tgcgattttt cccctagcag gctttttctg ctccgtcgtt
    29101 gccggagcac aatgggacat caataactag tatgttgtaa ctaaagtcgg atttttttaa
    29161 aaattacagg ccatcaagac cgcgaatcgg tttaatagtt gaccaggcac gacaagacgg
    29221 acaatgccag tagagggtgt atgcggtaaa accacatttc tggcagctat aacgaggctt
    29281 actacgcacc ttctcgccaa ccatgtcacg cagcaccatc agactctctt tggcgcgccc
    29341 ttcttccgct tcatttaagt ggtaatccat tagcttatgg aatacacgca tggtcggatg
    29401 acgctgaagc tggcgtgtaa tatagacctg tgcagcctca ctaccgtcgc gcgcttcgat
    29461 gatatcagca agcatcaatt cagcatcggc accggtgttc tcttccaccg cgcgttgcag
    29521 gaattctgcc cattcggcag ttttacccaa ctgctgatag caggtttgca acatttccag
    29581 cgtttcgctg accagttctc tgtcctggga tatgacgcgt tgcaaacttt cgacggcttt
    29641 ggcgtattct cctttcgcca taaacacgcg tcccatcatg atggatacgc gggcgctgtt
    29701 tttatctgcc gccgcccctt tttttagcaa ggtcatggca cgatcgagat cgtcgctggc
    29761 catatgctgc agggctaact cacagtagaa atgggcaatt tcgacgcgct gtttatcttt
    29821 acccagcttc accaggcgtt cggcaacatc aattgctttc tgccactcac tggtagcctg
    29881 gtagatttgt agcaactgtt gcagcgcgcc aatgcggaag tcagtttcat cggtcagctg
    29941 attgaacatg tcttccgcgc ggtcgtataa cccggcggcc atgtaatcac gccccagttg
    30001 ttgaatcgcc aacagacgct gttcataggt cagcgaggcg ctttccatta gggtctgatg
    30061 gatgcgaata gcgcgatcaa cttcgccacg cgaacggaac aggtttccga gcgtaaggtg
    30121 ggcttcaacg gtgcctgtat cctctttaag catatcgaga aacaggtcta ccgctttatc
    30181 ctgttgatta ctaagcagga agttaacccc cgctacgtaa tcacgcgaca agcggttggc
    30241 ttcatcttgc ttgttttgtt gcgcacttct gcggcccata taccagccat aggcagcggc
    30301 tacaggcaaa agcagaaaca acaactccag catagaaagt tattccttcg ccgctgacga
    30361 gtgcggcact acagccacgt cagtcgcggg tgaaagctgg ttttccagtc gctttatttt
    30421 acgttcagcg cgcgccaggg aaacacgaac tcgcagccag aacaggccac aaatcaacca
    30481 accgatagca aaccccgcag caaacaatac cgccagcaat gtggaaatac ggtactcccc
    30541 ttgcgctaac agataattaa acgtcacctg ttgatcgttc tgcgcaccca acgtgaccga
    30601 aatcacgaag atcgctaaca ccagtaagaa aatgagtaaa tatttcacat tacttcccgt
    30661 tatgtggtta gagcgaataa attatggtca acttaccgca atagcccttt aaagtaccat
    30721 tttcgcgccg tctgcgaaat ggcatccacg cgcattgttg atgatttaag gcctaaattc
    30781 ggaaaatcaa ccagcgttaa ctttcttgtt ctcgttgtgc aatttcgcga ttttcttccg
    30841 caggtggtgt taatggccca caaattcgtt gcgcaagcca cgttgccacc gccaccagcg
    30901 cccacgaaat caacgtagct accaccagat cgcgtggcca atgcatcccg agcagcaggc
    30961 ggcttcccat gactcccgtt gcccagacca acaagatagc aatggttaac gttcgccgac
    31021 gcggccacag caaaccaacg gccagcagtg cccaactggc agcaaacatc gtgtgaccgg
    31081 aaggaaaggc aaaccccgtc tctttctgcc agtgtgaacg caaatattgt ggaatatttt
    31141 tctcttcagc caactgttct tttactagat ttccgcgttc tgctcgcttt aaagtgtaga
    31201 actcatcaac cggaatatga tgtgtttttt ccaaccagat aacaaaaggt cgtggttcct
    31261 gtactttgtc tttgatccag gatttaacgc cttgtccaac aaggattgcc gccgccagaa
    31321 tggcaaataa tacaaaggca gccttaatgc gaaaacgcag acaccagaga aaccagccga
    31381 acaaaatcaa atgtgtaatg acgccccagg gctgggtgac agtttcagta acccaaaaag
    31441 ccgcttttag tagccaactt tgttctccag gttgccaacg ccagccagaa atccatacgg
    31501 ctactggcat gacaagcaat agtgcagctc ccactgcggt acgtcgggca atcgaacgca
    31561 tggcctctcc tttttgataa gtcccacaat cataactgaa aatgccagtt ccaagaaaaa
    31621 ttgacagatt tgtgccattc cgtgaacgat cgacgcgtcg tgattaggtg aaccccttct
    31681 cgttatggca aaataagcca atacagaacc agcattatct ggagaatttc atgcagctta
    31741 aacgtgtggc agaagccaaa ctgccaaccc catggggcga tttcctgatg gtgggatttg
    31801 aagaactggc aaccggacac gatcatgttg cgctagtcta tggcgatatt tccgggcata
    31861 ccccggtact tgcgcgcgtc cattccgaat gtctgaccgg tgacgccctg ttcagcttgc
    31921 gctgcgattg tggcttccag ctcgaagcgg cattgacgca aattgccgag gaaggccgtg
    31981 gtattttgct gtatcaccgt caggaaggtc gtaacattgg tctgctgaat aaaatccgcg
    32041 cttacgcact gcaggatcaa ggttacgata ccgtagaggc taaccaccag ttaggcttcg
    32101 ccgctgatga gcgcgacttc actctttgcg ctgatatgtt caaactcctt ggcgtcaatg
    32161 aagtccgctt gttaaccaat aacccgaaaa aagtcgaaat tctgaccgaa gcagggatta
    32221 atattgttga acgcgtacca ttgattgtag gtcgtaaccc caataacgaa cattatctcg
    32281 ataccaaagc cgagaaaatg ggccatttgc tgaacaaata accctcttgc atcgtgtaat
    32341 tcatttgctt gccggaagca aaataaccgg caagcaaata cttgttactt caacatatta
    32401 cgaatgacat aatgcaaaat gccgtcgttc tggtagtagg tcaactccgt cgcggtgtcg
    32461 atacgacaac ggcagggtac gacttcctgg ctaccatccg cgcgcgtaag cgtcaccgga
    32521 accgtcgcgc caggttgtag attttgcaga tcgccaatat caatcttctc ttccccggtt
    32581 agccctaacg ttttacgcgt cacgccttgc ggaaattcca gcggcaggat gcccatgcca
    32641 attaaattcg aacggtgaat tcgttcaaac gattcggcaa tcaccacacg aattccaagc
    32701 agacgaggtc cttttgccgc ccagtcacgg cttgagcctg atccatactc tttcccggca
    32761 atcaccgcca gcggcgtttg ctcctgctta tagcgcatcg cagcatcata aatagagact
    32821 acgtcgctgt caggtaaatg ccgcgtcatc cccccttcaa cgccaggcac catttcatta
    32881 cggatgcgaa tattggcgaa ggtgccgcgc atcatcactt catggttacc acgccgcgaa
    32941 ccgtaggagt taaagtcttt tcgctcaaca ccccgacctt gtagatatcg acccgctggg
    33001 ctgtcgggct taatactgcc cgccggagag atatggtcag tggtgactga atcccccagc
    33061 attgcgagga tccgcgcacc gtgaatatct tccactggtg ctggtgttgc ctgcatttca
    33121 tcaaagaaag gcgataagcg aatataggtt gagtcctcct gccaaccgta ggtatcggat
    33181 cgtgtgacgt taatttcctt ccactctgct gtgccttcaa aaacttctgc gtactctttg
    33241 cggaacattt ctgtggagac ttgttctacc gcacgggcaa tttcttgtgc cgatggccag
    33301 atatctttca gataaaccgg ctcgcctttg cgatcatggc cgataggctc agaagccagg
    33361 ttgatattca tatttcccgc cagcgcatag gcaaccacca gcggtggcga ggccagccag
    33421 ttagttttaa ccagcggatg gatacggcct tcaaagttac ggttgccgga cagcaccgca
    33481 ccgacggtta aatcgccttt tttgattgcc gtttcgatag gatcgggcag cggcccagag
    33541 ttaccaatac aggtggtaca accgtatccc acaaggttaa accccagttc gtcgagatac
    33601 ggtgtcagtt tcgcttttgc cagataatca gaaacgactt tcgaacccgg tgccaacgac
    33661 gctttgaccc acggttgccg cttgaggcca agagttacgg cttttttcgc cagcaagcct
    33721 gcggccatca gcacacttgg gttagaggtg ttggtgcacg aggttatcgc agcaatgacc
    33781 acagcgccat caggtaactg atactgatgt ccgttcataa cataatcgac tggcaggcga
    33841 tctttatgcg tggcattcac tttcagttcg ttactggcgg caaatgcttt tggtacatcg
    33901 ggcagtgcaa cgcgatcctg tgggcgtttt ggccctgcca ggctcgcttc aacgtcattc
    33961 atatccagtt ctaacatact ggtaaaaatc ggttcatcgc ccgggttacg ccacatgccc
    34021 tgcgctttgg catatttttc gaccaactcg acctgatctt cgctgcgccc gcttaaacgc
    34081 atgtaatcga gggttactgc atcgattggg aagaagccac aggtggcacc atattctggc
    34141 gacatattgg caatggtggc gcgatccgcc aacggtagtg aatccagacc atcaccataa
    34201 aattcgacga atttccccac cacgccatgt ttgcgcagca tttgggtaac agtgagaacc
    34261 aggtctgtgg cggtaatacc ttcacgtaat tttccggtaa gtttaaagcc cactacgtcc
    34321 gggataagca tggaaaccgg ctggcctaac atggcggctt ctgcttcgat cccacccacg
    34381 ccccacccca gcacgccaag gccgttgatc atagtggtgt gcgagtcagt accaacgagt
    34441 gtatccggat aagcaatcca ttcaccgtcc tgcaattcgc tccacactgc tttgccgaga
    34501 tattcgaggt taacctgatg gcaaatgcct gtgcctggcg gcacgacgct aaaccgactg
    34561 aacgcttgct ttccccattt caggaacaca taacgttcgt ggttgcgctc catttccagg
    34621 cgtacgtttt cttcaaatgc ctcatcatca ccaaaacgat cgacggtcac cgagtggtca
    34681 atgaccaggt cgaccggtga gagcgggtta acctttgcag tatcgccacc gaggcgttta
    34741 accgcttcgc gcattgccgc cagatcaaca acggcaggta cgccggtaaa gtcctgcatc
    34801 agcacccttg ccgggcggta ggcaatttca cggtcagcat gggcattttt cagccatccc
    34861 gccagtgcgt ggatatcctc ttcggtaact gagttaccat cctgccagcg cagcaggttt
    34921 tcgagcaaaa ctttgagtga cttgggtaga cgggtgatat cgcccagtga tttagcagca
    34981 agcggcaggc tgtagtagtg gtaagtttta tctttggcct gcaacgtgtc cttactggct
    35041 tctcgtaggg ttgacgacat agctcctcct taatgacagg gttgcgaagc cctgattctc
    35101 ttcagggtga atattaaaga taacacaaac atcgcgtaac gatttgataa caacccaaat
    35161 tgataaaaga ggagaaacct tgtggcaaag caaatgacaa ccccgccgca gcggggtcaa
    35221 ggatcagtga gtaatgatcc agaggagttc ggcccagaag agcatcgaca gagaaaacgc
    35281 gcccatccag gaccaatatt tcatatttgt gttgatcata ggctacaccc ttaacggtta
    35341 ttattttgtg ttgagataac tcaagctgtt attgctgcgg tgtattaaaa gccctttaat
    35401 tcattaaaaa tgggtaataa gaatgatgat aattattgtt tcggctgatc atcttcagca
    35461 aacagttcta taaaggcttg ctgctgcgat gttagttgcc agtcgtctgg aataacgacg
    35521 gaacattccc gtttcgtttc tggttcattg tcactatgct ggcataatgg tgacgactcc
    35581 agttgctcct gaccgaccat agttatcccc acactttcca aatcagcagt gcgacaacca
    35641 cccagaaaag tgcagaacca agaaaaaccg ccagccaggc tttacgtttt aattcaggat
    35701 cacggcgggg ttcctgattt ccagaaggca ttgctaacct catataatcg ccatcactta
    35761 tcagcaagac gttgaatgat tggtgtaatt aatcatacga tgagacaaat cctaaagaaa
    35821 ctttagtcaa aaatccagcg aatttacgaa tcgtctccag tgaataattc aatcttttga
    35881 cctgaaatag atatttgaaa actggaattt gcgaggcggg taattagaca ctactttaca
    35941 ggtaatttac cctataaata tgagggataa aaatattgtg cacggtatag aaataagtaa
    36001 caaaagagaa tatattccgg caccttcgct acataaaagg tgccgaaaat aacgcaagaa
    36061 attatttttc cggcagtttt atatctttaa acatgacctc aatttcttca ttggagcgca
    36121 atgcgacagc cgcatcaacg acatcacgcg ttaaatgcgg tgcaaaacgc tgaatgaaat
    36181 catacatata actgcgcaaa aaagtactac ggcgaaaacc aattttggtt gtactgtggc
    36241 tgaagatatc gtgagcatca acgcgcacaa ggtcgggatc ggcgaccgga tccaccgcca
    36301 tgctggcaat gacccctacc cccagcccta accggacgta agttttaatg acgtcagcat
    36361 ccgttgccgt gaaaacgata cgcggcgtta accctgcgcg gttaaaggca gtatccagtt
    36421 ctgaacgtcc ggtaaagccg aaggtatatg tcaccaacgg atattgcgcc agttcttcaa
    36481 tggtaatggc ttttttgcct gccagcgggt gatccggagt gactacaata gcccgattcc
    36541 agtggtagca cggtaacatc actaaatctt catacagatg cagcgcttct gtggcgatag
    36601 cgaaatcggc attgccttta gagacggcat cagcaatttg tgtcggcggg ccctggtgca
    36661 tatgcaaaga aacgcgggga taacgctcaa taaagccttt gataacgttt ggtaacgcgt
    36721 agcgtgcctg ggtatgcgtg gtggcgatat acagcgagcc tttatccggc caggtgtgct
    36781 caccggcaac cgattttatg gcatcgactt tcgacaggac ttcgcgagcg atacgaatta
    36841 tttcttgtcc tgctggcgtt acctgcgtca ggtgcttgcc gcttcgggaa aaaatttgaa
    36901 tgcctagctc gtcttccagc attctgactt gtttactgat cccgggttgt gatgtgtaaa
    36961 gtccttccgc tgttgatgag acattcaggt tatgattgac cacctcaaca atatagcgaa
    37021 gttgttgtaa tttcatgtta aaccatccac ttagacgcca tcagaacatc gttttttcgt
    37081 cacacctggc gggtgactaa tacgatttaa taataaaaaa ggtgctaact ataaccacta
    37141 tatcatttat agccttagtg tataggtgtg aagaaaaaat aatatgcaat aaaaaagggc
    37201 cgctttcgcg accctttgtt tataaaaacc tgacagaatt aaaggttatt tttttccttc
    37261 aacccatttg ccatcaacat aaaatgctga ccagccagtc gcctttccgt ctttttccga
    37321 agagacatat tgctgtttgg ttttacggct aaagcgaacc atggtcttat taccttccgg
    37381 atcctgctgt ggcgcatcgg ccagataacg cagtttttcc ggcagacggt cgcggaagcg
    37441 ataaagctct tccaccagtg gcgcacgcgt ttcacgcgat ttcgggaaag tgttggcagc
    37501 caggaacaca ccggcagcac cgtcacgcag cacgaaataa gcatctgatt tttcgcacgg
    37561 cagctcaggt aatggcaccg gatcttcttt cggtggtgcc acttcaccgt tacgtaaaat
    37621 cttacgtgtg tttttacact cttcgttggt gcaggccatg tatttaccga aacgccccat
    37681 tttcaggtgc atttcagagc cacatttttc acactcaacg atcgggccgt cataaccttt
    37741 aatgcggaat tcgccctctt cgatctcgta accgtcgcag gttgggttat taccacagac
    37801 atgcaactta cgtttcggat cgatgagata gctgtccatc gccgtgccgc atttcgggca
    37861 acgacgtttt gcgcgcagcg cgttggtttc agcatcttcg ccttccagca cgttcagcac
    37921 ttcgttttcc ggcaccaggt taatggtggt tttgcaacgc tctttcggcg gcagcgcata
    37981 gccagaacag ccaaggaata ccccggtact cgctgtgcga atccccattt tgcgaccaca
    38041 agtcgggcag tcaatgctgg tcagaaccat ctggttcggg cgcataccac cctcttccgg
    38101 atctttttca gctttatcta actgctgggt gaaatccgag aagaagtgat ccagtacagc
    38161 tttccactct gcttcgtgat ttgccacctg gtcgaggctg ttttccatct gcgcggtaaa
    38221 gtcgtagttc attaactcgc ggaaattttc ttcaaggcga tcggtgacga tttcgcccat
    38281 tttttccgca tagaaacgac gattttctac tcgcacatag ccacgatcct gaatggtcga
    38341 aatgatcgac gcataggtag acggacgacc gataccacgt ttttccagtt ctttaaccag
    38401 cgatgcttca ctgaaacggg ctggcggctt ggtaaagtgc tgggctggtg ttagttcaac
    38461 gagcgtcaga gcatcgcctt tatcgactgc cggtaagata cgatcttcat cgcctttacg
    38521 cagtgcaggc atcacttttg tccagccatc aaagcgcaaa atacgaccgc gtgctttcag
    38581 gcggaaatcg cccgcaccaa cggtcagcgt cgtggagtca tatttcgctg gggtcatctg
    38641 gcaggcaacg aactgacgcc agattaactg gtacagtttc tgcgcatctg cttccatatc
    38701 cttcagcgat tccgccatca cattgacgtc agaaggacga atcgcttcgt gcgcttcctg
    38761 tgagttttct ttgctggcgt actgattcgg actttccggc agatatttct taccaaaatt
    38821 atcgctgata taaccgcgaa ccatatttac cgcgtcctga ctcaggttag tggagtcggt
    38881 acgcatgtaa gtgatatagc ctgcttcata caaacgctgc gccatcatca tggttttttt
    38941 cacgccaaaa ccaagacggg tgctggcagc ttgttgcagc gtagaggtaa taaaaggagc
    39001 gccaggttta ctggttgtcg gtttgtcttc acgttccagc acgctgtagc gcgctttttc
    39061 cagcagactt accgcagcct gagtttgttc tttgttgacc ggacggaacg gtttgtcatt
    39121 ctgatgagtc acctgcaacg ccaacgcttc accagatggc gtagtcgtgc tggcatcgac
    39181 ttcccagaac tcttccggta cgaacgcttt aatttcacgc tcacgctcta ccaccaggcg
    39241 gaccgccacc gactgcacac gaccggcaga cagaccacga gcgatctttt tccacagcag
    39301 cggcgaaacc atatacccca ccacgcggtc cataaagcga cgcgcctgct gggcattaac
    39361 acgatcaata ttcagctcac ccggtttgtt aaatgcctgg cggatcgcgt ttttagtaat
    39421 ttcgttaaac accactcggc tatagcgcgc atcatcacca ccaatcactt cccgcaggtg
    39481 ccatgcaatg gcttcccctt cgcggtcaag gtcggttgcg agatagatgt ggtcggcttt
    39541 ttcagccagt tgtttcagtt cagagacgac cttctcttta ccaggcaaca cttcatagtg
    39601 cgcctcccaa ttgtgccacg ggtcaacccc catacggttg acgagagcgc cacgttcatc
    39661 ctttttaggc tttttagccg tcttggtgga ggtagagtcg gcactctttt tggcagctga
    39721 gccactggtc ggcaaatcgc ggatgtgacc gacgctggat ttcaccacgt agtcacaacc
    39781 cagatacttg ttgatcgttt tggcttttgc cggggactca acgatgacaa gagctttacc
    39841 catattcacc ttacctaatt tgattcttcc aggaatgcgt cgcacgttga taaaccttcc
    39901 actggcaacg agttaccgat atggactcgc tctctgcgga tatcaacccc ttgtcgtgac
    39961 tctgttcacg aaaattcagg tgactgagtt aacaggtctt ggcctacagg cgctatagca
    40021 cgacggaatt ttggtcgaat gtcaagcaaa tctattgcca gattgacgaa agcgtcacac
    40081 tgtacctgat aaaatccgat acgcaacttt attagcatgc aaaatccaaa gttgccagta
    40141 aggcgctctt gccaccagcg tggtatattt gtctgcaagc catgcctggc gtagactaat
    40201 gtccttgttt aaggagtaaa taatgaacaa agagacacaa cccatcgacc gcgaaacgct
    40261 gttgaaagag gccaacaaaa tcattcgtga gcatgaggat acgctcgcag ggattgaagc
    40321 caccggcgtc acgcagcgta atggcgtgct ggtatttacc ggcgactact ttttagatga
    40381 acaaggattg ccaacagcca aaagcaccgc ggtgtttaat atgttcaaac acctggccca
    40441 tgtgctttca gaaaaatatc atctggttga ttaaagcaaa acgcgaggtc ttagcctcgc
    40501 gtttgtcttt tacatcaatg gcttttgacc ccgctgccac cagcgtagca acaatcgatc
    40561 ggcgctctct gccgcgctgc cggtgaatcg gtcaatgagt cgtttacgct gcatatagcg
    40621 tacattaacc acttcacggc cttccatcag gctaagaata acttcatcac tggtgttgat
    40681 ttcatcaacc aggcctttct ctaccgcctg ttgtccgtac cagtgttcac ccgttgccac
    40741 ctgttcaata tccagagacg gacgcatacg cttcacaaaa tctttaaata actgatgcgt
    40801 ttcgttcagc tcttcgcgga atttctcccg cccttcttcg gtattttcac ccagcaaggt
    40861 cagcgtgcgc ttatactgcc cggcggtgtg cagttcgata tcaatatctt tgcttttcag
    40921 gaagcggtta aagttgggca tttgcgccac caccccaatg gaacccacaa tagcaaacgg
    40981 tgcggaaaca attttgtccg ccacacaggc catcatgtaa ccgccgctgg cagcgacttt
    41041 gtctaccgta acagttaaag gaatgttttt atcacgcaga cgctgcagct gcgaagccgc
    41101 caacccgtaa ccatgcacca tgccaccagg gctttccaga cggagcacaa cctgatcctg
    41161 cggtttgaat gctgcgagta ccgccgttat ctcttcacgt agcgagttca cttcatgggc
    41221 gtccatgctg cctttaaaat ccagcaccca gacgcgaggt ttactgtcag ttgccacctc
    41281 gcccagtttg gctttcgctt ttgctgcttt cgcttcttgc ttgtgctttt tcttctgcgc
    41341 tttgtgccac tgtttttgct gatgtgagtc catcagcgcc gcggccagtt cttctttcat
    41401 ctccttatac tgttcgctga gattgttgac ccgtaactcg ccacgctggc gtttattacg
    41461 ttgagcaaca ttgacaataa tggcggcaat cgccgcaatc gctagcacaa cggtaacgat
    41521 tttagccaaa aacaaaccat attcagacaa caattccacg agtccccacc ttggttaaac
    41581 aacgctttgg ctcacagttt acaacaggca tgttaagccg tctcgcagag atgttattgc
    41641 agcgaaagta tgacaaagta tccattttgc ggtgttatcg ccccaggctg attgaaatgc
    41701 ccttctgttt caggcataaa gccccaaagt cataaagtac actggcagcg cgacagacaa
    41761 cgtcgttgag gagtagccat gcattaccag ccaaaacaag atttactcaa tgatcgcatt
    41821 atcctggtga cgggagccag cgatgggatt ggtcgtgaag ccgcgatgac gtatgcacgc
    41881 tatggtgcga cagtgattct gttgggccgt aatgaagaaa aattacgtca ggtagccagc
    41941 cacataaacg aagaaactgg gcgtcagcca cagtggttta ttctcgattt gctgacctgc
    42001 acgtccgaaa attgccaaca actggcacag cgcattgtcg ttaattatcc gcgtctggat
    42061 ggtgttttgc ataatgccgg attgctcggc gatgtttgcc caatgagcga acaaaatccg
    42121 caggtctggc aggacgtcat gcagatcaac gttaatgcca cctttatgct cacccaggca
    42181 ctgcttcctt tattactcaa atcggacgcc ggttcactgg tctttacttc atcaagcgtt
    42241 ggacgtcagg gacgagccaa ctggggtgca tatgcagcgt cgaaatttgc caccgaaggg
    42301 atgatgcagg tactggccga tgaatatcag cagcgcctgc gcgtcaactg cattaaccca
    42361 ggcggtacgc gcaccgcaat gcgcgcaagc gccttcccga ccgaagatcc acagaaactt
    42421 aaaacacccg ctgatatcat gccgctctac ctctggctga tgggcgatga cagccgccgt
    42481 aaaaccggca tgacctttga cgcccaaccg ggccgtaaac caggaatttc ccaatg
//
LOCUS       DAJTPG010000026        40546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTPG010000026 DAJTPG010000000
VERSION     DAJTPG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..956)
                     /locus_tag="OO230_001453"
     CDS             complement(<1..956)
                     /locus_tag="OO230_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5691270.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OO230_001454"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OO230_001454"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5691271.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO230_001455"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO230_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO230_001456"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO230_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5691272.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OO230_001457"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OO230_001457"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5691273.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OO230_001458"
     CDS             complement(5018..6346)
                     /locus_tag="OO230_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5691274.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="OO230_001459"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="OO230_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO230_001460"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO230_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5691275.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO230_001461"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO230_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5691276.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO230_001462"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO230_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5691277.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO230_001463"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO230_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5691278.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO230_001464"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO230_001464"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5691279.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="OO230_001465"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="OO230_001465"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5691280.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="OO230_001466"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="OO230_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5691281.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO230_001467"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO230_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5691282.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="OO230_001468"
     CDS             18267..19166
                     /locus_tag="OO230_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5691283.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="OO230_001469"
     CDS             19172..21604
                     /locus_tag="OO230_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5691284.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="OO230_001470"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="OO230_001470"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5691285.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="OO230_001471"
     CDS             22717..23982
                     /locus_tag="OO230_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5691286.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="OO230_001472"
     CDS             complement(24223..25815)
                     /locus_tag="OO230_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5691287.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="OO230_001473"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="OO230_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5691288.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="OO230_001474"
     CDS             complement(27012..28130)
                     /locus_tag="OO230_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5691289.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO230_001475"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO230_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5691290.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="OO230_001476"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="OO230_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5691291.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="OO230_001477"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="OO230_001477"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5691292.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO230_001478"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO230_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5691293.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="OO230_001479"
     CDS             complement(31379..31444)
                     /locus_tag="OO230_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691294.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="OO230_001480"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="OO230_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5691295.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="OO230_001481"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="OO230_001481"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5691296.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="OO230_001482"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="OO230_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5691297.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="OO230_001483"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="OO230_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5691298.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="OO230_001484"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="OO230_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5691299.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="OO230_001485"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="OO230_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO230_001486"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO230_001486"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5691300.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /locus_tag="OO230_001487"
     CDS             39719..39979
                     /locus_tag="OO230_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5691301.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40546
                     /locus_tag="OO230_001488"
     CDS             40244..>40546
                     /locus_tag="OO230_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5691302.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10096 a        10719 c         9868 g         9863 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcctg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaatg tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcgtg
//
LOCUS       DAJTPG010000027        38787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTPG010000027 DAJTPG010000000
VERSION     DAJTPG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO230_001489"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO230_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5691303.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            531..572
                     /gene="hisL"
                     /locus_tag="OO230_001490"
     CDS             531..572
                     /gene="hisL"
                     /locus_tag="OO230_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5691304.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="OO230_001491"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="OO230_001491"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5691305.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="OO230_001492"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="OO230_001492"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5691306.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="OO230_001493"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="OO230_001493"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5691307.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="OO230_001494"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="OO230_001494"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5691308.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="OO230_001495"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="OO230_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5691309.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="OO230_001496"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="OO230_001496"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5691310.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="OO230_001497"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="OO230_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5691311.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /locus_tag="OO230_001498"
     CDS             7140..7751
                     /locus_tag="OO230_001498"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5691312.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO230_001499"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO230_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5691313.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO230_001500"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO230_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO230_001501"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO230_001501"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5691314.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..12304)
                     /locus_tag="OO230_001502"
     CDS             complement(11945..12304)
                     /locus_tag="OO230_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691315.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12954..13517)
                     /locus_tag="OO230_001503"
     CDS             complement(12954..13517)
                     /locus_tag="OO230_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691316.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13732..14661)
                     /locus_tag="OO230_001504"
                     /pseudo
     CDS             complement(13732..14661)
                     /locus_tag="OO230_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067396.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14724..15098)
                     /locus_tag="OO230_001505"
     CDS             complement(14724..15098)
                     /locus_tag="OO230_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5691317.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="OO230_001506"
     CDS             complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="OO230_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5691318.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="OO230_001507"
     CDS             complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="OO230_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5691319.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17168..18058)
                     /locus_tag="OO230_001508"
     CDS             complement(17168..18058)
                     /locus_tag="OO230_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5691320.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="OO230_001509"
     CDS             complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="OO230_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5691321.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="OO230_001510"
     CDS             complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="OO230_001510"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5691322.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="OO230_001511"
     CDS             complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="OO230_001511"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5691323.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="OO230_001512"
     CDS             complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="OO230_001512"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5691324.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="OO230_001513"
     CDS             complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="OO230_001513"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5691325.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23204..24097)
                     /gene="galF"
                     /locus_tag="OO230_001514"
     CDS             complement(23204..24097)
                     /gene="galF"
                     /locus_tag="OO230_001514"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5691326.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="OO230_001515"
     CDS             complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="OO230_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5691327.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="OO230_001516"
     CDS             complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="OO230_001516"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5691328.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="OO230_001517"
     CDS             complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="OO230_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5691329.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="OO230_001518"
     CDS             complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="OO230_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5691330.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="OO230_001519"
     CDS             complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="OO230_001519"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5691331.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="OO230_001520"
                     /gene_synonym="manB"
     CDS             complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="OO230_001520"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5691332.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="OO230_001521"
     CDS             complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="OO230_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5691333.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34285..35508)
                     /gene="wcaI"
                     /locus_tag="OO230_001522"
     CDS             complement(34285..35508)
                     /gene="wcaI"
                     /locus_tag="OO230_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5691334.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="OO230_001523"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="OO230_001523"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5691335.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="OO230_001524"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="OO230_001524"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5691336.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="OO230_001525"
     CDS             complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="OO230_001525"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5691337.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38102..38650)
                     /gene="wcaF"
                     /locus_tag="OO230_001526"
     CDS             complement(38102..38650)
                     /gene="wcaF"
                     /locus_tag="OO230_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5691338.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38666..>38787)
                     /locus_tag="OO230_001527"
     CDS             complement(38666..>38787)
                     /locus_tag="OO230_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5691339.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10353 a         9715 c         8730 g         9989 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat
    12181 agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat aactcactat
    12241 attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat
    12301 tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaaa
    12361 taatacacac aaggataaaa tatcgtccca acttgtaatt tgtaatgttg atatctttta
    12421 ttaaacttgc aattgagatc gcaacctgca atactgacac tgggtaaatg ggtgataaat
    12481 acctgtcacc ttgcgtaaca gaaaaccaaa ataagacata tgttaaaaca atcgagaaaa
    12541 atacccaaag tattttatta ttcctaagtg taaagacaga cagtatagca taaaaaacaa
    12601 catcactgcc aactttatta attgcttgtt gcaaatctaa tgtggcagga cttataccat
    12661 gtcgagcttg ttctgctgtt tgaacttgta gatcaatttc attccatagg tagtgaccaa
    12721 atatatttcc tccagcagga ctaaaaggat caccagagat ccaaaaacta cggacatacc
    12781 aataaatgcc accaagaatt gttgccaagc taaaaataag aaatgtctgt atgtttctct
    12841 ttttcgctat caatataaca aatatgatgg ctccatatat ggcaccaaat agttttacgc
    12901 cacaagcaat tccaataaaa aaaccacata aaaaaattaa ttcattttgt gttttattgt
    12961 tttctgagcg aatatatata agaatactgg ctgcagcaaa acaaagatag cgttaaatca
    13021 atatatgcat aaccaagatg atctttaagt ggattgatag taaatagtag gattgtagca
    13081 ataatgcctg gataataagt tttcgtaatt tcccatgaac agccaacaag tccacaggct
    13141 attacaaaaa aacagatatt tgctaaaaac tgagctaacc ttacatctcc taactgtaaa
    13201 cctaaagcca ttaaaagatc tgcattttgc gggaacagag gaaaacgcaa atattgttca
    13261 acaacaattg catggtgctc aactaatgaa cgagccaaag gtaagtgata catagtatca
    13321 tcccaatcac cgggagctcg cagggacgat aacaatgtaa ccacaaaaat caaaacaaga
    13381 aataaaataa caaacagcgt actgaactca atattattta ttgccagcca tctaaattgc
    13441 tgacgtttaa taatgcttaa aacccaacac accgtaaaaa ttaaaaagat agcaatctta
    13501 ttaaattgcc cgaccatgcc cagaaagtaa attatcaaaa tatttaaaga catccctatg
    13561 cataggcaat aaaaaataaa gaaaatttgt taagtcctgc caccccctcc ttaaaaaaag
    13621 atgacaatac aatattacca atgacaaaaa atgtcattcc aaaagcaaaa caatacacaa
    13681 catacaaata caacggcaat tcaatcatag taaaatactc tatttacatt atcagtttat
    13741 agttttttca gaaagataaa ataatgcctt catttcacgt ctagttgttg tatttgaatg
    13801 caaaataatg ccacatacaa atgccattat tgacgataac atgatggagg cagttaaaaa
    13861 ggcagtcgga aatcttctca ccaaacctgt atcaatataa tcaacaatga tgggcaatcc
    13921 taaaattata gacaaagcag caagtaaaac gccgataata ctaaaaaagt atagtggccg
    13981 ttcaagcgag taaagtttaa ttattgtttt cagaatcttg aacccatcac tccaagttga
    14041 tagtttgctc acactccctt cagatcgctc gccatatttt gtattaacct caccatactt
    14101 catacgtaat tctaaagcat gaattgtaag ctcagtttcg atttcaaatc cccttgatag
    14161 acatggaaaa gatttaacat agcgtctgga aaatacacga taccccgaca acatatcagt
    14221 aaaattccct ttaaagatag tctgaacagt accagtcaac aatttatttc ctcagcgatg
    14281 cccttttctg tatgtatttt gatctccatt ctcttgacga catcctacaa ccatatccaa
    14341 ttgatttttt atgagatggt taatcatttc tggagcagaa cttgcatcat aagtgtcatc
    14401 tccatcaacc attagataaa tatccgcatc tacatctgag aacatacgtc ttacgacatt
    14461 ccccttgcct ttcagaggaa cagagtgaac tgttgctcca gcatcctctg ccacttgtgc
    14521 tgtactgtca gtcgaattat tatcaaatac atatatagaa gcagttggaa tattcgtttt
    14581 gaaatcattt atgacttttg ctattgcctt ctgctcatta taacaaggaa taattacagc
    14641 gatattaaaa gagtgataca taagcccctc atataaacta tttagctaat gtgcaagtaa
    14701 ttgtgttttt aacaaatcgt ttactaacga aaaatgaaat attttgatgc gcaatatcct
    14761 aaaaccaaac taaaaagaga aaaggtgaac aatgtaaata ttggagataa agctaagaga
    14821 tcaaataaaa cgccgaataa ataactcatc gcccccataa aaaatataaa tataaaatat
    14881 ctagccttgg ttgcagatac tttaaatgag catcttgcat ttgcaaaaaa agaaaaggtt
    14941 gctgcacata taaaaccaac aatgttactt cggccttgac caaaagctaa attattataa
    15001 cacgcataaa atacccccca atgtaaagca gtattgacaa ttccaattaa aaaataacta
    15061 aaaaaagaac tggtcaataa cttccctatt tttaacatcc tttattttgc tccagaagtg
    15121 aggttattac taatttggat attttctata gaaaataccc ataaaactac aacgcctata
    15181 ataaaaattc ccatgggtgt aaaaagaaat ggattagtta tagaaacggt aatactgaaa
    15241 aaaagtatta acgacatcat taatgatgtt tttgtactat ttaaaaaata gcctttcaaa
    15301 aagataagca acataggaac taatgatata aatacaccaa taacgcctgt tttctgaagt
    15361 atctcaagag gtactatttc aatccttaat cgatctaata taaatgaacc aaatcctctt
    15421 ccaaacaaga acgtcgctaa atcaacattt ttataataaa aatataaatc attaaatcta
    15481 acagagtcag aatcttcact gcgtgatccg accatgtaaa gcataattat aaatagaacg
    15541 ctgcccaata ttattataat agtatttaaa tatagcttat gtaatttaaa actggctaat
    15601 aacagtgata atgttgtaaa aagtaataca cctcttgatt ctgtcaataa tatcgcaagc
    15661 actattatga gacattttaa atattttttg tcgacaaaac tgataatcgc accgacacca
    15721 aaaaagtaga aggatttgga aaaaaaagcc ccatcgggac gaaaaaagaa ctcgctagtc
    15781 aatgataagt gttcataaat taaattaaaa tttaacaaac cgaaattcaa caaaattatg
    15841 tatgaaatat ataacagtga tccatataat gcaaatgcat taactatttt aataaaaatc
    15901 tcacatgaaa atacttttaa agtatatctt agtgtaaata gatatataag tgacagcata
    15961 aaaaggtatg gttgcacatc aaacagtata tcatttattt tattaccagc taataagccg
    16021 acaaagaagc cataaaaagg aaataaaagc aaccatataa aaagactgac acatttttgg
    16081 gtgattgaaa aattgaatgt aactaaatta aaaattaaaa gtataaaact aaatacaaat
    16141 aataaatatc gcaaagggaa tattcccaat ggctccagta atcttccacc accaccaata
    16201 atcagttcaa tacataaaaa tgttataaaa attttattta tattattcat attcgtaagg
    16261 tgatgttttt taaaatatgt ttaaaatcaa cattaataaa atataaattt tatcagcctt
    16321 tttccgcctt aacttccaaa aacttaaagc caagatcaaa ttgcctatat aacctttacc
    16381 caattgatta ttcacaaagc tatcatcagc taaggcatta gctattttag ttacttcttt
    16441 tcgataccac ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt
    16501 tgctcccact tggttgtttt catgctgtct gtacagcatt gttggttttc tatcaatata
    16561 ccacgaataa tttctcgtcc gcgcaaatga atataaaaac caatcatgca gacagatatc
    16621 ttgtgcagca tttttattat ttatgatgaa cttcttaaat tcaataagcg tttctttttt
    16681 tataacatat gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga
    16741 ctgagcctta tcaactaaat tttttcgccc tgatggataa tatgcaatta cattgctgga
    16801 ataacaattc gcattattaa ataccaagca gtcaattgca ttttttattt tatctttata
    16861 ccaaatatcg tcctgatcac taaaacagat ataatcatag ttttcaagat cgacatcttt
    16921 aattaatcta taaaaatttt tacctgcccc cccaaactta ttgacgcttg gaagtatatt
    16981 tataattgag ggatatgata attgaaattc ttcacaaata tttaatgtat tatcagttga
    17041 taaatcatct gatataaaaa ttgagatgtc tacatctttt tgttcaataa tactttttaa
    17101 ttgttcctca atccagcatt cgccattata cgtagccata agtacagcta ctcttttttg
    17161 atcagaacta tgcattataa cgaccgcccc cagtaattcc tcttattcca tccagcgtag
    17221 ccaaaatatg ttttttcata tattcaattt tatttttttg agttgcgata attaaagtcg
    17281 tatgaattaa agaaaaaaca acttccctaa tagagagtaa aagaggcaca tgattttttc
    17341 taagcaaaag aaatgcattt cttactcgat aataacgacg tactggcgag tgaattggta
    17401 tgttaatccc acagattttt actgacttat ccccaatagc atgaaccatt tctatatcag
    17461 gaatgatatg aactaatatg ccatagttta agcatctaag acaccattct gtatcgacat
    17521 aatcaataaa aagttcctca tccatcattc caacaatctc aaaaacttct tttgaaacca
    17581 tggttccgga tgagattgtt actgaacttt taaacggtgt ctgtccttca gtgacatgaa
    17641 ttttctctcg aagaccattt tttttgatat tacaaatagg ataataatgt gacttatctc
    17701 tatcaataaa aactgggcca gctatttttg cattttcatt atccatatag caggaaagtt
    17761 ttttgataaa ctcattgcga atacggctat cttgatcaaa aaaaattatc ttctctgccc
    17821 ctttttcaag agaatatcga atcccaatat tttgtgcttt tgctatacct tcattcctac
    17881 caagacaaat taaatgaact ttttcaatgt ctgctaattt atctttcaag gtgaaattag
    17941 tcctattatc gactactaca acgtaacaat tttgctcctt tagttcagtg atcagtgcat
    18001 tcagattttt aagttcggga ttatatgtca caatgacagc gtaaatatta ctattcatta
    18061 tccaagtgac tcagtaattg gttaatttta gttttcatat acagaacaca ttctagtata
    18121 aagccattgt aataacagag catctaaaat tacacgtatg ctccaggata ttgcggcacc
    18181 cagtaaccca tatttttcga ccataaaata caaaagggct aaataaggaa ccaattcagc
    18241 acaatgaatc aaagcagtaa ttttagattt tcctaatgcc tggattgcag agaatggtat
    18301 cattgccagc gcattgaaga aaaaaccgat caataacaca tttaatatat taaaggaatt
    18361 aatttgataa ttctcaccaa accatatttt caatacaagc cctgaatata acaagcctat
    18421 gattatcacc ggcaaacaga ttagaaacat taaaagcaat gatttattta cattacgttt
    18481 aaattcttta aagtctttga tgttacttaa ccttggaaac actgccctcc cgattgctgc
    18541 aggtattatt cctaatttta gaataacctc cgctggtgca gaataaaatg caactttatc
    18601 tgcccccata atatttgaca ctataaatcg atcaaaatac accatgaccg gacttatgat
    18661 attacttact gtcatccaac cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa
    18721 atgaaccccc gaaattttaa tatcattcct gacataatag gcagagacta aaatcgaaat
    18781 aactcttgca aatattaaac cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa
    18841 tatggccgga attcccgcga tgcaagagct tgatatagat ttttgaatat ttacaatgcc
    18901 aaatttttca tccccctcaa gaatggctga ccataattga tttagaataa ataatggaat
    18961 gcaaatagcc aacagtttaa atgctagttg tatatcacta tgttcaacac cagaaatttt
    19021 tagataatta acaattccat cagagaaaat caataataaa aaagcaccaa agcatgaaaa
    19081 tagcaccaaa aaagatgtac ttgttgaaat taccttggtt ctttcatgat gattatcacg
    19141 atgaattgca atttctctaa taacagagcg agtcaggcct acatcaaaaa ttccagcata
    19201 cccaacaagt gcaattgcaa ttgtataaac accgaaacct tcaggcccca aacctcgagc
    19261 gagaaatcct agcgagggga tagcaattaa agttggtatt gcatagccaa aaaggttcca
    19321 gacactattt tttattatgc tcattattac atttctttat catataaaat aatcagcatc
    19381 tacaaatttt tgaccattaa tatctttatc agaaagcaat ggattttgaa ccggccattc
    19441 tatatttata cttttgtcgc tccataaaat cccacgctct tgcatttttg aatagaaatt
    19501 atttgttttg tagacaaatt ctgcttcatc actaagaacg caaaacccat gagcgaatcc
    19561 ttccggtatc cataattgac gcttattctc cgccgacaaa tttacaccaa cccacttgcc
    19621 aaatgttggt gatgatttcc gaatatcaac agctacgtca aaaacttcac cgacaacaca
    19681 gcgtaccagt tttccttgag cataaggttc cagctgatag tgcagtccgc gtaacacacc
    19741 cttagttgat ttagaatggt tatcctgaac aaactcaacc tttcgtccta cagcctcttc
    19801 gaaaactttc tgattgaagc tttccataaa aaagccccgc tcatcaccaa atacttttgg
    19861 ttcaaaaatt agcacatcag gaatttcagt tttaattacg ttcattttat taataacctt
    19921 taatcatttt cagcagatac tgaccataag catttttctt cagcggctcg gctaatacct
    19981 tcacttgctc agcatcaata aagcctttac ggtgagcaat ttcttccggg caggaaactt
    20041 tcagcccctg acgctcttca attgttgcaa tgaagttgct tgcttcaata agactttgat
    20101 gtgtccccgt gtccagccat gcataaccac gccccatcat agcgacagac aaacgtcctt
    20161 ggtccatata aatacggtta atatcggtaa tttccagttc gccacgtgca gatggtttaa
    20221 ggtttttcgc catttctaca acgtcattgt catagaaata aagcccagta accgcatagt
    20281 tactttttgg ttcctgcggt ttttcttcca gactaattgc cgtaccgtta tcatcaaatt
    20341 ccaccacacc gtagcgctca ggatcattta cgtgataggc aaaaaccgtt gcgccactct
    20401 ctctgttaac agcggtatcc attaatttcg gcaggtcgtg accatagaag atattatcac
    20461 caagtaccag cgcacaatca tcaccaccta taaattcttc accaatgata aatgcttgcg
    20521 ccagaccatc cggactcggt tgtactttat attgcagatt aagcccccac tggcttccat
    20581 caccaagcaa ttgttgaaaa cgtggtgtat cctgcggcgt actgataatc agaatatctc
    20641 gaatacccgc caacataagc gttgaaagcg gataataaat catcggttta tcataaatcg
    20701 gtagcagttg tttgctcaca gccattgtca caggataaag acgagtgccg gaaccaccag
    20761 ccagaataat acctttacgc gttttcattt caccattcct tttaattcat ctcgctctag
    20821 catcatgagc gagatgcaat aatttgttaa attgccgtgg tcgtaaataa ttcgttgagc
    20881 atacgtttca cgcccacctg ccagtcaggc aagacaagcg caaagttctg ctgaaatttt
    20941 tcggtattga ggcgagaatt atggggacga cgtgctggtg tgggataggc cgttgtcggc
    21001 acggcattaa gtttgttaag agcaaggttg atccccgctc tgcgcgcttc ttcaaatacc
    21061 agagcggcat aatcgtgcca ggttgttgta ccaccagcaa ccagatggta caaaccagca
    21121 acttctggtt tatttgcggc cacgcgaatg gcatgagcgg tgcaatcagc cagtaattca
    21181 gcccccgttg gtgcgccgaa ttgatcgttt attacagcca actcttcgcg ttcttttgcc
    21241 aggcgcaaca tcgttttggc aaagttattt cctttaccag cgtataccca gctggtacgg
    21301 aaaataagat gctttgcgca atgttcttgt aacgcttttt ccccagctag cttggtttca
    21361 ccgtagacat ttagcggtgc tgttgcatcc gtttccagcc atggcgtgtc accgttgcct
    21421 gggaaaacat aatcagtgga gtagtgtata acccaggctc cgacttcatt agctgctttt
    21481 gcaatcgctt cgacgcatgt cgcgttaagt aattgcgcga aattcggttc tgattccgct
    21541 ttgtctactg cagtgtgagc agcagcatta acaataacat cggggcgaat ttttttgacg
    21601 gtttcagcca caccttcggg gttgctgaaa tctccacaat aatcagtgga gtgaacatcg
    21661 agcgcaatca agttacccag aggagccaga gcacgctgca gttcccaacc cacctgccct
    21721 gttttgccga aaagcaggat attcattact gacggccctc atagttttgt tcaatccacg
    21781 actgataagt accgcttttc acattctcaa cccaatttgt attagccaga taccattcca
    21841 ccgttttacg aatcccgctc tcaaacgttt cttgcggttt ccagcccaat tcgcggctaa
    21901 ttttatcggc atcaatggcg taacggcggt catgccccgg gcgatcggca acataagtaa
    21961 tttgctcacg ataagatttc tctttcggta caatctcatc caacaaatca caaatagtga
    22021 gcactacgtc gatgtttttc ttttcgttat gtccaccaat gttataagtt tcacccgctt
    22081 taccttcggt tacgacggta tataacgcac gagcatgatc ctctacatac aaccagtcgc
    22141 gaatttgatc ccctttacca taaataggta atgccttacc ttccagagca ttcagaataa
    22201 ccaatggaat aagtttttcc gggaagtgat aaggaccata attgttcgag caattagtca
    22261 caatggttgg taaaccataa gtccgcttcc aggcacgtac caaatgatcg ctggaagctt
    22321 tagacgcaga atacgggcta cttggcgcgt aagctgtcgt ttccgtaaat agcggtaacg
    22381 cttcgttatt atttgcttca tccggatggg gtaagtcacc ataaacttca tcagtagaaa
    22441 tatgatgaaa gcggaaactt tttttctttt catcattcag agcagaccag taattgcgcg
    22501 ccgcttctaa aagaacataa gtccccacaa tattggtttc aataaatgcc gcagggcccg
    22561 ttattgaacg gtcaacgtgg ctctctgccg ccaggtgcat caccgcgtct ggctggtgct
    22621 gtgcgaaaat acgcgacatg gctacagcat cgcagatatc tgcatgctca aaagcataac
    22681 gttcagaatc agaaacatca gcgagtgatt ccaggttccc ggcgtatgtt aatttatcga
    22741 cattaacaac actatcttgc gtattattta ttatgtgacg aacaacagca gaaccaataa
    22801 accctgcgcc accagtaaca agtatcttca cttttttatt ccatatagct atagagcatg
    22861 ctgtgaaata gactgctcca gatttgatta atagaggtat taatacacgc taccgcccct
    22921 ggcttaacag ctaccagtgc actgcatgca tctcaacgat gtggcgagcg ttagccactc
    22981 gcgccaaatc cgaaaaattc aaacgttaat tgtcttacca aacagccgta gaaacaagga
    23041 aaatcctgga aaaatttgaa taatatgagc tatgtaactt cttgttttta taggctttat
    23101 aaaaacggca acggcaggaa aattcaatga gcaaaactta tgccacgaat cttcactgcc
    23161 gttataattt tcttatcaac cgttacatcc ggtcagattt tcattattcg cttaacagct
    23221 tctcaatacc tttacggaac ttcgcccctt ctttcaggtt gcgtaggcca tacttcacaa
    23281 acgcctgcat atagcccatt tttttgccgc agtcgtaact gtcgccggtc atcagcattg
    23341 catcaacgga ttgttttttc gccagctcgg caatagcatc agtcagctga atacgtcccc
    23401 atgcaccagg ctgagtacgt tccagttccg gccaaatatc ggcagaaagc acatagcgac
    23461 ctacggccat gatgtctgag tccagcgtct gcggctgatc cggtttttcg ataaattcaa
    23521 caatgcggct gactttaccc tcacggtcca gcggctcttt agtctggatg acggagtatt
    23581 cagagaggtc acccggcata cgttttgcca gcacctggct gcggcccgtt tcgttgaaac
    23641 gtgcaatcat ggcagcaagg ttgtaacgta gcgggtcggc gctggcatcg tcgatcacaa
    23701 cgtctggcag taccacgaca aatgggttgt caccaatggc aggtcgcgca cacaaaatgg
    23761 agtggcctaa acctaaaggt tcgccctgac gcacgttcat aatgatcacg cccggcggac
    23821 agatggactg tacttccgcc agcagttgac gcttcacgcg ctgctcaagg agtgattcta
    23881 actcataaga ggtgtcgaag tggttttcga ccgcgttctt ggacgcgtga gttaccagga
    23941 ggatttcttt gatccctgca gccacaatct cgtcaacaat gtactgaatc attggcttgt
    24001 cgacgattgg tagcatctct ttgggtatcg ccttagtggc aggcaacata tgcatcccga
    24061 gacccgctac aggaataact gcttttaaat tcgtcattat ttcatccacc tgtaaaatgg
    24121 ttgctgaatt atagcttgtt cgattttttt cgccagcatc aattaccctg aattgattac
    24181 tgaattactt gtgatgttac gccgcttcgt tgtggattgc agtagcattg ttcctaagta
    24241 tggctccatt tttccaggaa tggtcgcaaa tctactccct ccgttccggc aatctaaagt
    24301 taatcttttc cacattaaca atatggtgat taatcctgtc gatatcgacg gagctttgcc
    24361 ctctttcatt caccgcatga acatttgcaa gagacagcag tgtttctttt ttcgccataa
    24421 agacgccgcg aacgtctttg cgcatgtcga agttcatgct caatgctggt ccaactgagg
    24481 attcctgcat cacattgata tttcgcataa aaagatgttg cggcttgttg tgtaactcca
    24541 gcgaggcacg cttcatctca atgttagtta gcgacacaaa ggagacggca ttcccggcgg
    24601 agatttggat gccgcgcaat ttataagcaa gatgggtatt atccagttga atattattca
    24661 ctcggaaatt ttgcggtatc gagagatatt tgcctttaat taccccatag ccgattaaca
    24721 tcccggcgct attaatcatt tcaatattat caatcacgaa attgtcacaa ccgtaaatag
    24781 cgactgtcgc gttatcaatg cccgctttct tactgaaatc cggcgtgata ttgcgggcat
    24841 tgatattacg aataacaaaa tgtttaccat tttcaacatg gatcaactgc cgacaatccg
    24901 atcccgtgat attcgccacg acaaagtttt tcactgcctg gtcttccggg tagttgttat
    24961 cataagtgct tcccgcaagg cctatgccga tgccccagtt gattttgccg ttggtacagt
    25021 tgatacgctc gatgacatgg tcagatatca aaatatcact gtcgttaatt gccacgttcc
    25081 attcaatggc gtcgccctgt aagtcgctga acttacagtt ggtaatgttg gcaccgataa
    25141 tctggttatg aaatccctgg cgtaagatgg cgtaattagc gtggctgaca gtgaggttat
    25201 cgatggtcag gttgcgcatg acccgtttgt ttttgccgcc aatataaatc tgcattaccg
    25261 ggccaaagcc gctcatcgcc agccctttga tggtgcagtc agaaccacgc acatccaggg
    25321 taatgttatg cataccgccg ccctctcccc ctgtcacctg gctgccgtcc tgtaagacaa
    25381 atcgccctct gccgttgccg cgcaggcttc caaggatgtg taacgttttg ccgggaggga
    25441 tgaagatgcc ggtgttgata ttgtcacaaa cgaatccggc aggcacgacg accgtttgcc
    25501 cttcgctgaa ggcttgttta aatgaggcga tccagtcatg tgggttgtag tcgttaatgt
    25561 taacgctttg tcgggcggga agtgcgcgag cgaaaggggt atggaggaag gcaagcgccg
    25621 agcttgccgt caggaaggtg cgtcgggaga gtgttttaaa tggcatacgt tctcctctat
    25681 aaagcctgca gcaagctggc gagttctcga ttaatcacct gctggttaaa atcgtgttcg
    25741 actttttcgc acgcacgttt gacgaccgga gccagttcgt cggtatccag ttggctaaac
    25801 gccgccagac gttgtgccag tgcgcgagca tcgttctcag gcagcagcca gccggattta
    25861 tcggcctcca ccagttcagg aattccgcta tgcagagtag aaaccaccgg aataccgacc
    25921 gccatcgctt ccattagcgc caccggaatg ccttccatat cgccatccgc gcccgttacc
    25981 gatggcaaca ggaagacatc cgcgtcgtcg agcatctcct tcacttcatg gctcggttta
    26041 aagcccggca tctctaccac atcttccagt tgatattgtt cgatgatggt gcgcaggcgt
    26101 cgttcccaca ggccaatgcc gaggatgcga tagcgaaatg ccacgccctg ctctttcaac
    26161 tgacggcagg cttcaatcgc cacatgcagg ccttttttct cggttaagcg tgcgacagat
    26221 atgatttcca gcggcgttgc gggcgctttc accggacgcg ggctaaagcg cgtcatgtcc
    26281 acgcccatac gcgatacggc gattttttct ctggggcagc ccattttttg cagccttccg
    26341 gcccacagat cgcttatcgg taacatcagg tcgccacggc gaaacagttg ttgatattcg
    26401 ggagtgtagt ggttgagcac ttcccgactg gagatatcaa taccgtggaa gatggtggca
    26461 attttgccgc gaatgatacc cagttcgcgt agttttgctg cggttacccc cgccgggcca
    26521 aaatgagcga tgaacacatc ggcgcgaaac ggtgttgcga cctgaccaca aatggcagac
    26581 aaaatcaggt tccgcgactc ggcaccatag cgtttgaggt tgagcgcatg ccaggtattt
    26641 ttacgatgaa tgccacgtaa ggtctggctg gcgcggtggc gcagtttcgc tactttgccc
    26701 gcaggttcgt cctgtaacca gcgggttctg gcgacaaggt tgtatttcgt ccatgccgcg
    26761 tgggtgttct ctgtatcgcc tttttgcaaa gcgacaatct ccacttcaaa gcccatatca
    26821 ataaacgcgg taatttgatt gaggacgaag gtttccgacg acagcgggaa tttcaataag
    26881 aagaagccga ccttcatttc acctccccga tgcgctcaag cacagactgc accatctgca
    26941 ttcccgtctg acgttcgcga ctgacggctt cgttaaatcg agcgttcagc accggaagct
    27001 ggcctaaggt atccgcaacc atcgcttgca ggctgccgtc cagtaaatga cgaatatcaa
    27061 tcgccatctc tggcagtccc agctgctgca taatcccggc ggatttatgt tcatagttga
    27121 tggcaatcgc cggagtagca aagttcattg agataatggc ggagtgcagg cgcgtaccga
    27181 cggtgagttc acaggctccg agaattttgc ccatttccag atcgttgagt tcatccatca
    27241 ctacgtggta acgggcagga tcgctgatgt gctggcgcag attgagcgcc accatgcggt
    27301 cgtctttgtt atagctgtcg atacccgtac aggtggagag cgcaatcacc tgatagcctt
    27361 catcgagaat gcgattgacc accccggcaa aggctttttc atacgcttgt tgagtggtgc
    27421 cgagacgttt gtcgaacggt gccagttcgc gcagggtaat tgccactgtt ttctgttgtg
    27481 cggcaacgtc cagccagtgt tgaacggcat agctggcggt gaagtcttcg gtgtggtgat
    27541 cgaccagcca cgcggtatcg acgccatgtt ccacttttgc ggtggtgata tttctgcgtt
    27601 tcatcaaatc aaggctgacc gattcgcgca ggatcagcgc gtcgcagtga ccaaagacat
    27661 agttcgccag ttggttaaat tgctcatcct ggaaggggcc gacgctgtga ccaatcataa
    27721 acagcggctt tttcgccata aatgtgcaaa gcgcatgttc aaactgcggc acgccgtaga
    27781 gatcgacaaa aaacgatccg ccgacctgaa taatggcgtc gtaacctgac agcaggcgca
    27841 cgaagtcggt gaatccctgg gcgatggcga tattgcgcag cttgccagtg tcggtgacgc
    27901 gtgagagcaa tacctgatgc tggtagcggc gacggaggac ttttttaatg cgcccgacaa
    27961 cgcccgccgc gctattgtgt tgtgtcatct gcaggaacag cggatcgccc attaccgggc
    28021 ggttgagcag ccaggaagaa ctgaccggat agcggctcat cacgtccact tcggtatgtg
    28081 gattgaggat gttgatggca tcaagtaagc cgcgcaagat ggcgctgtcg ccacgattgc
    28141 cgcaagtgtg gttgcccaga ataagtaatt tcattttttc ctcataaatt tgatgccagg
    28201 tgaggctgtg tttgttgtgt tcgttgccgt tttttgcctg atgcgacgct ggcgcgtctt
    28261 atcaggccta caggtcccga gcacagaacc gtaggacgga taaggcgttt tacgccgcat
    28321 ccggcaaccg ttgtcggaac cgaaaacagc aattcacccc gcccgtaaaa gcattttcat
    28381 tttttcgctg cgacaaaact gacgcttcac ttccaccacc agcggatggc gtgacagcac
    28441 aatcatcacc acaaacgcca gcacgcccgc ggctatttgc accgccagca gcatccccag
    28501 cgccagttgc cctttcagca caatgcccag cgcataactg accaccagcg tcggcaacga
    28561 gagataaaac ggcagccaca aactcaggat gtactggcga taactggaac caagaaccgg
    28621 tttaatcatc acgaagtaac tcagaatggt gttgataatc tgcaccagca ggaaaccgag
    28681 cgtgacgccg attgcgcccg ccatctgccc accaataaca atcgccggaa taaacagaaa
    28741 tgttttgaat acgttgaatt taaagctgat atcgacccgc gctttcgcca tcagcagcga
    28801 accaatcggg ttacccacgg agcgcagcag acccacaata cacagcaatt gcagcaccgg
    28861 aataatgctg ttccactttt cgccgaatac cagcggtacg aagttattcg acaccaccat
    28921 cagccccagc agcgccggaa agttgataat ccccactatc gacagcagct tgtagaagtt
    28981 aacacgcagc ttttcggtgt cgtcctgaat tttggcaaac gccggaaaca acacgcgggt
    29041 gatgattggg ttcagcttca tcggcggtac gacggccacg ttgtacgcca ggttgtatcc
    29101 ccctgccacg cccgcgccaa gaatacgcgc cagcaccagc gttgaaaggt tggtgttgag
    29161 atagttgatg atgctgtccg ccgtcagcca ggcaccaaaa cgtaagttcg gtgccacaga
    29221 cgccagcgag aagtgcagac cggggcaata aattttgcgt ccaaagtagc caaacagcag
    29281 tgttctcacc gcactattga ccagataacc gaggatcgcg gtcatcgcca gcggccagaa
    29341 atgggcgcta actaccgtaa aagtgaagcc cgccagcacc gcgctggttt cgatcatgcc
    29401 gattttgttg aactccagct ccttctgcat caacgcgcgg aactgttgcc cgtgggggat
    29461 caccacaaac gccagcgaca atgttttaat caacggtgcc aggtccgggt tattcagcac
    29521 gtcgccgatg acatcactca acaaaaacac cgccacacat accacaagcc ccagcccgac
    29581 gttcagccag tacagcgtgg tgagttcaag gtgactgatt tcttttcgct gaataatcga
    29641 gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg ataatcacca gcgacacagt
    29701 cagcaggccg aactggtggt tgtcgataat ccgcgccagc acggtcatct gcaccagccc
    29761 gaggccgatg atgatcaccg tggcaatagc cgaccacttc gcgccgctga tggttttttc
    29821 acgtaagctc atatcaatat gccgctttgt taacgaagcc cttgaatacc gtcaggaaaa
    29881 cgattttgat atcgaaccag acgctccatt cgcggatgta ctcaaggtcg aactcgacgc
    29941 gtttttccat tttctccagc gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc
    30001 cggtaatgcc cggtttcact ttatggcgca gcatgtagcc ttcaatgagc tggcgatact
    30061 gttcgttatg cgctactgcg tgcggacgtg gaccgacaat cgacatcccc ccggtcagca
    30121 cattgataaa ctgcggcaat tcgtccagcg aggtgcggcg cagaaagttc cccactttag
    30181 tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt gtcgttctcc atcactttca
    30241 tggaacggaa cttccacact ttgatcggct tgccatccat accatagcga gtctggcgga
    30301 aaataaccgg ccctggtgaa ctgagtttca ccgccagcgc aatacagcac agcaccgggg
    30361 agatcagcag cagaataagc gtcgccagca caatgtcttc cgcacgtttt agcaggcggt
    30421 taacccccga aagcggcgtg tcatacagcg gcaccaccgg aacgccgttc atctcttcga
    30481 gacgtgaatg gagaatgttg aaggtaaaga cgtcggggat cagcagcacc gaacaggtgg
    30541 tatccgccag ttgatggacc agttttttca ctcgtgcgcc gtcgcacatt tgcatcgcga
    30601 tatagacgtt atgaatcttg cccgcttttg catcctcgac cagctgttgc aggttacccg
    30661 cccagtcgtt agaaacgccg cccggtttcg ggtcgtggta aacgcccacc acttcaaacc
    30721 ctaaccacgg ctgattacgg aaactctcca tcagcatttg tccggcggct aaatcccccg
    30781 ctacggcgac catccgcttg ttgtagccat gattacgcag ccagcccgcc ccaatgcgaa
    30841 tacatgaacg gcaaaccacc agtccgatgc tggtcagccc ataccacgcc agccagattt
    30901 tcagttgcgt gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta
    30961 aggtccagtt ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc
    31021 gataaaaatc ggtgatgccg cccagcatct ggaacaccac cagcgtaatc agcgccacca
    31081 acaggtgcat gtagaggaat gacagtccgc tgacttcgca aaccagccat agcccggcaa
    31141 acatgatggt gatatctgaa aagcgttgca ccatagagat taacgatgca ttggttttcg
    31201 ctcgctcgcg cttttttaga tttgtcatcg ttgttcctgt tattagcccc ttacccgcag
    31261 tggggtaagg gaagatccga cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg
    31321 cttccatcag cggcacatca ccgcgcgatt ccacattcag gcgcaccacc ggttcggtat
    31381 tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcgctgcgat
    31441 ccaccgccag cgcctcacgg ctaaaatgct gttccacgcg gttaatcgcc tcaacgggtt
    31501 gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca
    31561 gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca
    31621 tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt
    31681 agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt ttcgacatta
    31741 ccggcgtgcc gcctgcagca gtcaccacat caatagtgtt ccaagagaga cgcggatcat
    31801 ggatgatctt cgcgccggga tttttttcga ggaacgcttc tgccagcagg ccgacaatgt
    31861 agtagccctc gataaactgc cctttttcgt caaacagaaa acagcggtca aaatcgccat
    31921 caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg tcgtcgcgac
    31981 attccggcag cagcgggtta ggaataccgt tggggaaatt gccgtccggt gtgttgtgta
    32041 ctttgattaa ttccacgggc gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca
    32101 ccggacccgc tgcgccattc ccggagttga tcaccagctt gagcggcgtg agatttttga
    32161 cattgatata accgaacagg tgatcaacgt aagcgtcacg caggttgatt tgctgatagc
    32221 gaccgcgttt ggtttcatcg acgggaggaa agtcgttggc ttcagccaga cgctggacgt
    32281 cgcgcagtcc ggtatcaccg ctgatcgtgc gagccccctc gcgcaccagc ttcatgccgt
    32341 tgtaatccat cggattatgg ctggcagtaa cttcaatgcc gccatccacg ccgagatgga
    32401 acgtggcgaa atagatctct tcggtgccgg acataccgat atccagcaca tcgacgcccg
    32461 catcctgtaa accttttgcc agcgccagtt ttaaggtttc gctggtgaga cggacatcac
    32521 cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca atgcgccagg
    32581 cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg
    32641 ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc gaaacggcca
    32701 gttttatttt tgtcgttatt tacaccgcgg tttcgcattc attgcctgat gcgacgctgc
    32761 cgcgtcttat caggcctaca aaaccgaacc gtaggtcgga taaggcgttc acgccgcatc
    32821 cgacaaacag cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg aagcgcacca
    32881 catcatcctc ttcgagataa gagccggagc gcacttcaat taaatcgagc ggaattttcc
    32941 ccgggttttc caggcagtgc gtcgccccca gcggaatata aatggactcg ttttcaccaa
    33001 gcagtttgat atcaccatca atggtgactt ttgccgttcc cgcgaccact acccagtgtt
    33061 ccgcgcggtg atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt
    33121 tcacctggta gcggtcgccc gcgtcgatag agtcatattt gccccacgga cgatacactt
    33181 cgcgatgcac ccgatgctca tggcgaccat cggctttgat ctgctcgacc acttttttca
    33241 catcctgtac cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc accactacca
    33301 gatctttcac cccgacggtg gtgaccaggc ctgattcggc atacacatag ctgttttcag
    33361 ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct
    33421 cccataatga agaccaggaa ccaacatcgc tccagcccgc atccatcggc accacaacag
    33481 catctgccgt aggttccatg accgcgtaat ccaccgactc ttccgggcag gcgagaaacg
    33541 cttcttcatc cacacgaata aaatcgagat ccggatcgac ggcgctcatc gctttttcgc
    33601 aggcatcgag gatatccggg cgatattttt tcagttcttc gagatagcgt ccggcgcgga
    33661 acaggaacat gccgctgttc cagtaatatt cgccgcttgc tacataggcc tgggcggttt
    33721 ccagattcgg tttttcgaca aactgcgcca cttcaaaggc caccgtatcc tgctcaccca
    33781 cagacacttc accgcgacga atatagccat aaccggtttc tggtagatcc ggcacaatgc
    33841 caaaggtcac cagcttgccc gcttcggcat aaggcatggc attacgcacg gcggcacgga
    33901 acgcgtcttc atcggcaatc acatgatccg ccgccaatac cagcattaac gggtcgctct
    33961 ccgggctatg acgttttgcc gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc
    34021 ctgccggttc gagaataatg ttctcggtga gtttgttcag ttgacgcagc tgttccgcga
    34081 caataaagcg gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc acgccgttca
    34141 ggcggcagat ggtggtttgc agcatggtga gatcgccttt caggcataaa aactgcttgg
    34201 ggtaaagtac gcgggaaagc ggccataaac ggctaccgga gccacctgcc atcacaactg
    34261 gatagagttt cgactgcgcc ataattatcc ccgaatatca tttataaatt gacgtaacac
    34321 gttctcttta tcgagcgtgc gttcggcata ttcacgtgcc accgtgttat gtttgggcag
    34381 caggagcgcc tgacgaatcc ccgccaccag cgcctcgacc gattccggtt ctacgcaaac
    34441 cgcaataccc ggaaaggttt cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat
    34501 caccgcgtta ccgcctaccg ccagaatatt ggtcagtttc gacggcaata cggcatctgc
    34561 cgcgccgcgt ttttgcacca ccagatggca atcgcccatc ttcagcagtg caggtaaagc
    34621 gtcatacgat tgcagcggga aaaattgcat gttgcgcagt ccacgctgct gcgccatttt
    34681 ttccagccgc gctttgccgc cgccctgccc gacaatggca aaaatcagcg gttcatcgcg
    34741 caggagatcg gcagcttcaa taacgttttc cagcccctgc ttttcaccaa tattgccgga
    34801 gtaaagaatg atttttttgt tatccggcag gccaagctgg ttacgaaggg catcaacatc
    34861 ggcgtctgca acatgctgaa aacgggcaat ttccgaccag ttggggaaga agatgacgtt
    34921 atccgccgcc acgccttttt cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac
    34981 gttatcgacg ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg ccactttgcc
    35041 gcctttgcct tttccggcaa ggcccagccc cagcatggcg tccacttcgt aatcctgaat
    35101 atgcagcacg gtacgcgcac cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa
    35161 cagcgttggc actacgccaa taatgcgatc cggcttccag cgacgttgcg ccatcagtgg
    35221 aaagaaactg ctgacggcaa aactgccgag atgcaacaag cgtttcaggg tgctcggctg
    35281 ttttggcacg tacagcgggc agcgccacac cgtggcggcc ccttcttctc gtttgtagcg
    35341 ccaggcggaa tagttctcac ccacctgcca ttgcgggtag taaggcggtg cggtaatgac
    35401 ccgcacctca tgaccttgtg ccgccaacca ttccaccatc tcgccggtgt atttgccgat
    35461 gccggttaac tccggcgagt agttaatgcc gtagaccagt attttcataa tccgggtact
    35521 ccggcacgct tctcagcgag aaaataggct cggctgttag cgtgaacatc gttactggcg
    35581 agcagcgcgt ccggcgtcag ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc
    35641 tcttcttccg ctactctgaa gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg
    35701 ccggagaagt tatcgtcata aaaatgctgc cagacaccgt aaaactggcc tgctgttatc
    35761 ggcagacgca gccccagttc cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg
    35821 tctttctgca cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg gttggttctt
    35881 ttgccaagca gaaactcgcc gcgactgttc tcgacaataa agtcgagaga gacaagcggt
    35941 gtggagcgca ctacagtggc aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc
    36001 ggtcttgatt ctcaaggaac cactggtaag tgctggcaag ccccgcttcc agtgagattt
    36061 cgtgatacca gccaagctga tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat
    36121 ccggtttgct ggcatcaaaa accacccgac ctttgtaacc caccactttg gcgatggttt
    36181 gcgccagctc gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg
    36241 gttgggtatt ctccagccag acttcatgcg ccagctccat gacatgaatg ctcgccgccg
    36301 ccatatcatc gacgtgcagg aattcacgca tcggtgtacc gctgccccat accaccacgt
    36361 ccggcgcatt ctgtgccgtc gcctcgtgga agcgacgcag caatgctggt atcacatggg
    36421 agttactcgg gtggaagttg tcatgcggcc cgtacaggtt ggtcggcatg acagtgcggt
    36481 aatcgcgtcc atactggcgg ttgtaagatt cgcacagttt gatcccggcg attttggcaa
    36541 tggcgtaagg ctcgttggtc ggctccagcg tgccctgcaa taactcgctt tctgccatcg
    36601 gctgttttgc cagtttcggg tagatgcagg acgatccgag aaacatcagt ttgttcacgt
    36661 cgttctgatg cgcggcgtga atgatgttgc tctcaatcat catgttctgg tagatgaaat
    36721 ccgccggata ggtgttgttg gcaacaatgc cgcccacttt cgccgccgcc agatagacct
    36781 ggtcaatgcg ttcgctggca aagaaatcat gcacggcgcg gctgtccagc aggttcagtt
    36841 catcgcgggt acgtaatacc agctccacat caccgcgctg ttcgagctgc cgcttgatgg
    36901 cagaaccgac catcccacaa tggccagcga taaaaatgcg ttgtttactc atgcttatga
    36961 ctccagcgcg atcgccacgt cgtagccgtg agatttcagc agagagtgtt ttttcgccgc
    37021 ttcgaggtca ttagccacca tttcagacac catctctctg agggtgattt ccggtttcca
    37081 gcccagtttt tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt ctgccggacg
    37141 gaagtaacgc ggatcaacgg cgataatcac atcacccggt ttaacgcccg gcgcgtcatg
    37201 cccggtgacg gaaaccacaa tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt
    37261 gatgcccagc tgtgctgccg ccatttccac aaactgacgt acggagtact gaacgccggt
    37321 cgcgataacg aaatcttccg gctgttcctg ttgcagcatc atccactgca tttttacgta
    37381 gtctttggcg tggccccagt cacgcaggga atccatattg ccgaggtaca ggcacgactc
    37441 cagcccctgg gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc
    37501 gcggcgcggg gattcatggt tgaagagaat tccgttacaa gcgtacatgc cgtaggattc
    37561 gcggtagtta acggtgatcc agtaggcgta cagtttggcg accgcatacg gagatcgcgg
    37621 gtagaacggc gtggtttctt tctgcggaat ttcctgcacc agaccataca gttcagaggt
    37681 ggaagcctga tagaaacgag ttttcttttc cagaccgagg aagcggatcg cctccagcag
    37741 gcgcagcgta cccatcgcgt cgacgtcagc ggtatattct ggtgactcaa aagagaccgc
    37801 aacgtggctc attgcgccta ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg
    37861 cgtcaggtta gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt
    37921 gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt
    37981 aataccatgc acctcgtaac ctttttccag cagaaactct gccaggtaag aaccgtcttg
    38041 tccggttaca ccggtgatga gagcgacttt tgacatgtct tattcctctg tatttttgaa
    38101 tttattcagt ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg caaaccacat
    38161 ttgccggaag cgatttaaaa acactgcttc gtgcacccac gacggtgccg tcgccgattg
    38221 tgacgcctgg ggcaacaaag acatcggttg ccagccagca tttctcgcca atcacaatag
    38281 gcgtggcgtt aatggtgaaa tgttgacttg catggtcgtg gctaccggtg cataaataac
    38341 ttttttgcga tatcaccgaa tgtgcgccaa tggttatttc accgagggta tataaattga
    38401 cgtcatcgcc aacccacgcg taatcaccta aggttaattt ccacggatag gtaattttta
    38461 ctgacggacg aataactacg ttttttccta tttttgctcc gaataaacgt aataaaaaag
    38521 cccgccagcg atacaatact tgtggcgacc aggcaaatat tgttgcctgt actgcccacc
    38581 ataattgcac tttaatagcg ttgccgcccc ggaacccttt cggcaccgag aaaccgctta
    38641 aatcttgcat tggttttcct tatattcaga ctttgttata taaggcttta gtcttacctg
    38701 tcgtacgttg gcgtaaatgc caggataatt ctgcccagaa gccaggtaca tgtaatatct
    38761 gccgttggac ttttttggcg tcagcac
//
LOCUS       DAJTPG010000028        38400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTPG010000028 DAJTPG010000000
VERSION     DAJTPG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            59..649
                     /gene="uidR"
                     /locus_tag="OO230_001528"
     CDS             59..649
                     /gene="uidR"
                     /locus_tag="OO230_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5691340.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1037..2740
                     /gene="uidA"
                     /locus_tag="OO230_001529"
     CDS             1037..2740
                     /gene="uidA"
                     /locus_tag="OO230_001529"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5691341.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            2746..3771
                     /gene="uidB"
                     /locus_tag="OO230_001530"
                     /pseudo
     CDS             2746..3771
                     /gene="uidB"
                     /locus_tag="OO230_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            3810..5075
                     /gene="uidC"
                     /locus_tag="OO230_001531"
     CDS             3810..5075
                     /gene="uidC"
                     /locus_tag="OO230_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5691342.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5301..6809)
                     /locus_tag="OO230_001532"
     CDS             complement(5301..6809)
                     /locus_tag="OO230_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5691343.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(6910..8085)
                     /gene="manA"
                     /locus_tag="OO230_001533"
     CDS             complement(6910..8085)
                     /gene="manA"
                     /locus_tag="OO230_001533"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5691344.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8284..9930
                     /gene="fumB"
                     /locus_tag="OO230_001534"
     CDS             8284..9930
                     /gene="fumB"
                     /locus_tag="OO230_001534"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5691345.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10073..11476
                     /gene="fumC"
                     /locus_tag="OO230_001535"
     CDS             10073..11476
                     /gene="fumC"
                     /locus_tag="OO230_001535"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5691346.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11473..12402)
                     /gene="tus"
                     /locus_tag="OO230_001536"
     CDS             complement(11473..12402)
                     /gene="tus"
                     /locus_tag="OO230_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5691347.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12478..13779)
                     /gene="rstB"
                     /locus_tag="OO230_001537"
     CDS             complement(12478..13779)
                     /gene="rstB"
                     /locus_tag="OO230_001537"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5691348.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(13958..15277)
                     /locus_tag="OO230_001538"
     CDS             complement(13958..15277)
                     /locus_tag="OO230_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5691349.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15502..16245)
                     /gene="rstA"
                     /locus_tag="OO230_001539"
     CDS             complement(15502..16245)
                     /gene="rstA"
                     /locus_tag="OO230_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5691350.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16374..16709
                     /locus_tag="OO230_001540"
     CDS             16374..16709
                     /locus_tag="OO230_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5691351.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(16706..17428)
                     /gene="folM"
                     /locus_tag="OO230_001541"
     CDS             complement(16706..17428)
                     /gene="folM"
                     /locus_tag="OO230_001541"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5691352.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17465..18847)
                     /locus_tag="OO230_001542"
     CDS             complement(17465..18847)
                     /locus_tag="OO230_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5691353.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19033..19977)
                     /locus_tag="OO230_001543"
     CDS             complement(19033..19977)
                     /locus_tag="OO230_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5691354.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20501..22033
                     /gene="pntA"
                     /locus_tag="OO230_001544"
     CDS             20501..22033
                     /gene="pntA"
                     /locus_tag="OO230_001544"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5691355.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22044..23432
                     /gene="pntB"
                     /locus_tag="OO230_001545"
     CDS             22044..23432
                     /gene="pntB"
                     /locus_tag="OO230_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5691356.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23457..24491)
                     /gene="tqsA"
                     /locus_tag="OO230_001546"
     CDS             complement(23457..24491)
                     /gene="tqsA"
                     /locus_tag="OO230_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5691357.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            24902..25267
                     /gene="mdtJ"
                     /locus_tag="OO230_001547"
     CDS             24902..25267
                     /gene="mdtJ"
                     /locus_tag="OO230_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5691358.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25254..25583
                     /gene="mdtI"
                     /locus_tag="OO230_001548"
     CDS             25254..25583
                     /gene="mdtI"
                     /locus_tag="OO230_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5691359.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25622..26443)
                     /locus_tag="OO230_001549"
     CDS             complement(25622..26443)
                     /locus_tag="OO230_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5691360.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26543..26626)
                     /locus_tag="OO230_001550"
     CDS             complement(26543..26626)
                     /locus_tag="OO230_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691361.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(26719..27054)
                     /locus_tag="OO230_001551"
     CDS             complement(26719..27054)
                     /locus_tag="OO230_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5691362.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27451..28704)
                     /locus_tag="OO230_001552"
                     /pseudo
     CDS             complement(27451..28704)
                     /locus_tag="OO230_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            28811..29740
                     /locus_tag="OO230_001553"
     CDS             28811..29740
                     /locus_tag="OO230_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5691363.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAELAKEPFVFFDPHVGTGLY
                     DDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAE
                     EDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            29875..31095
                     /gene="mlc"
                     /locus_tag="OO230_001554"
                     /gene_synonym="dgsA"
     CDS             29875..31095
                     /gene="mlc"
                     /locus_tag="OO230_001554"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5691364.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31220..31915
                     /gene="bioD"
                     /locus_tag="OO230_001555"
     CDS             31220..31915
                     /gene="bioD"
                     /locus_tag="OO230_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5691365.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(31868..33160)
                     /gene="clcB"
                     /locus_tag="OO230_001556"
     CDS             complement(31868..33160)
                     /gene="clcB"
                     /locus_tag="OO230_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5691366.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(33319..33933)
                     /gene="dmsD"
                     /locus_tag="OO230_001557"
     CDS             complement(33319..33933)
                     /gene="dmsD"
                     /locus_tag="OO230_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5691367.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(33976..34830)
                     /locus_tag="OO230_001558"
     CDS             complement(33976..34830)
                     /locus_tag="OO230_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5691368.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(34832..35449)
                     /gene="dmsB"
                     /locus_tag="OO230_001559"
     CDS             complement(34832..35449)
                     /gene="dmsB"
                     /locus_tag="OO230_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5691369.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35460..37498)
                     /locus_tag="OO230_001560"
                     /pseudo
     CDS             complement(35460..37498)
                     /locus_tag="OO230_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37559..>38400)
                     /locus_tag="OO230_001561"
     CDS             complement(37559..>38400)
                     /locus_tag="OO230_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCR5691370.1"
                     /translation="PNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQ
                     KFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPE
                     ANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQL
                     FGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPA
                     KVTPRILPGVSAMGQGAWHEPNMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEI
                     EKI"
BASE COUNT         9752 a         9302 c         9849 g         9497 t
ORIGIN      
        1 ataggcgctc acctgacaac gcggtaaact agttattcac gctaactata atggtttaat
       61 gatggataac atgcagactg aagcacaacc gacacggacc cggatcctca atgctgccag
      121 agagattttt tcagaaaatg gatttcacag tgcctcgatg aaagccatct gtaaatcttg
      181 cgccattagt cctgggacgc tctatcacca tttcatctcc aaagaagcct tgattcaggc
      241 gattatctta caggaccagg agagggcgct ggctcgtttc cgggaaccga ttgaagggat
      301 tcattttgtt gactatatgg tcgagtctat tgtctctctc acccatgaag cctttgggca
      361 acgggcgctg gtggtcgaaa ttatggcgga agggatgcgt aacccacagg tcgccgccat
      421 gcttaaaaat aagcatatga cgatcactga atttgttgcc cagcggatgc gtgatgccca
      481 gcaaaaaggc gagataagcc cagacatcaa cacggcaatg acttcacgtt tactgctgga
      541 tctgacctac ggtgtactgg ccgatatcga agcggaagac ctggcgcgtg aagcgtcgtt
      601 tgctcaggga ttacgcgcga tgattggcgg tatattaacc gcatcctgat cctctcattt
      661 cgcgggctgg tgataactgt gcccgcgttt catatcgtaa tttctctgtg caaaaattat
      721 cctccccgtg ttcggagaat tcccccccaa aatattcacc gtagccatat gtcatgagag
      781 tttatcgttc ccaacactct cgaacgaacg ttcgattgct tattttatgg cttctgtcaa
      841 cgctgtttta aagattaatg cgatccatat cacgctgtgg gtattgcagt ttttggtttt
      901 ttgatcgcgg tgtcagttct ttttatttcc atttctcttc catgggtttc tcacagataa
      961 ctgtgtgcaa cacagaattg gttaactaat cagattaaag gttgaccagt atttttatct
     1021 taatgaggag tcccttatgt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
     1081 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
     1141 cgaaagcgcg ttacaagaaa gccgggcaat tgcagtgcca ggcagtttta acgatcagtt
     1201 cgccgatgca gatattcgta attatgtggg taacgtctgg tatcagcgcg aagtctttat
     1261 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
     1321 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
     1381 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
     1441 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
     1501 gaaaaagcag tcttacttcc atgatttctt taactatgcc gggatccatc gcagcgtaat
     1561 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
     1621 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgata tcagcgttga
     1681 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcaccagcg ggactttgca
     1741 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
     1801 agccaaaagc cagacagagt gtgatatcta cccgctgcgc gtcggcatcc ggtcagtggc
     1861 agtgaagggc gaacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
     1921 tcatgaagat gcggatttgc gaggcaaagg attcgataac gtgctgatgg tgcacgatca
     1981 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
     2041 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgcagctgt
     2101 cggctttaac ctctctttaa gcattggttt cgaagcgggc aacaagccga aagaactgta
     2161 cagcgaagag gctgtcaacg gggaaactca gcaggcgcac ttacaggcga ttaaagagct
     2221 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
     2281 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
     2341 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
     2401 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggttggt atgtccaaag
     2461 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa ctgctggcct ggcaggagaa
     2521 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg gcctgcactc
     2581 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
     2641 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
     2701 gacctcgcaa ggcatattgc gcgttggcgg taacaattga cctacatggg cctcgggctt
     2761 tgctacagcc tggtgaatat tccttatggt tcacttgcta ccgcgatgac ccaacaacca
     2821 caatctcgcg cccgtctggg cgcggctcgt gggattgccg cttcattgac ctttgtatgc
     2881 ctggcatttc tgataggacc gagcattaag aactccagcc cggaagagat ggtgtcggta
     2941 taccatttct ggacgattgt gctggtgatt gccggaatgg tgctttactt catctgcttc
     3001 aaatcgacgc gtgaaaatgt ggtacgtatc gtggcgcagc cgtcattgaa gatcagtttg
     3061 caaaccctga aacggaatcg cccgctgttt atgttgtgca tcggtgcgct gtgtgtgctg
     3121 atttcgacct tcgcggtcag cgcctcgtcg ttgttctacg cgcgctatgt gttaaatgat
     3181 accgggctgt tcactgtgct ggtactggtg caaaacctgg ttggtactgt ggcatcggca
     3241 ccgcttgtgc cggggatggt cgcgaggatc ggtaaaaaga ataccttcct gattggcgct
     3301 ttgctgggaa cctgcggtta tctgctattc ttctgggttt ccgtctggtc actgccggtg
     3361 gcgttggttg cgttggccat cgcttcaatt ggtcagggcg ttactatgac tgtgatgtgg
     3421 gcactggaag ctgataccgt agagtacggt gaatacctga ccggcgtgcg aattgaaggg
     3481 ctcacctatt cactattctc atttacccgt aaatgcggtc aggcaatcgg aggttcaatt
     3541 cctgccttta ttttgggatt aagcggatat atcgccaatc aggtgtaaac gccggaagtt
     3601 attatgggca tccgcacatc aattgcctta gtaccttgcg gatttatgct actggcattc
     3661 gttattatct ggttttatcc gctcacggat aaaaaattca aagaaatcgt ggttgaaatt
     3721 gataatcgta aaaaagtgca gcagcaatta atcagcgata tcactaatta atattcaata
     3781 aaaataatca gaacatcaaa ggtgcaacta tgagaaaaat agtggccatg gccgttattt
     3841 gcctgacggc tgcctctggc cttacctctg cttacgcggc gcaactggct gacgatgaag
     3901 cgggactacg catcagactg aaaaacgaat tgcgcagggc tgataagccc agtgctggcg
     3961 cgggaagaga tatttacgca tgggtacagg gaggattgct cgatttcaat agtggttatt
     4021 attccaatat tgttggcgtt gaaggcggag cgtattatgt ttataaatta ggtgctcgtg
     4081 ctgatatgag tacccggtgg tatcttgatg gtgataaaag ttttggcttt gccctggggg
     4141 cagtaaaaat aaaacccagt gaaaatagcc tgcttaaatt aggtcgcttc gggacggatt
     4201 atagttatgg tagcttacct tatcgtattc cgttaatggt tggcagttcg caacgtacat
     4261 taccgacagt ttctgaagga gcattaggtt attgggcttt aacgccaaat attgatctgt
     4321 ggggaatgtg gcgttcacga gtatttttat ggactgattc aacaaccggt attcgtgatg
     4381 aaggggtgta taacagccag acgggaaaat acgataaaca tcgcgcacgt tcttttttag
     4441 ccgccagttg gtatgatgat accagtcgct attctctggg ggcatcggta cagaaagatg
     4501 tttccaatca gatacaaagt attctcgaga aaagcatacc gctcgacccg aattatacgt
     4561 tgaaagggga gttgctcggc ttttacgcgc agctcgaagg tttaagtcgt aataccagcc
     4621 agcccaatga aacggcgttg gttagtggac aattgacctg gaatgcgccg tggggaagcg
     4681 tatttggcag tggtggttat ttgcgccatg caatgaatgg tgccgtggtg gataccgaca
     4741 ttggctatcc cttttcatta agtcttgatc gtaaccgtga aggaatgcag tcctggcaat
     4801 taggtgccaa ctaccgtgta actccgcaat ttacgctgac atttgcaccg attgtgactc
     4861 gcggctatga atccagtaaa cgagatgtgc ggattgaagg cgcgggtatc ttaggtggta
     4921 tgaactatcg ggtcagcgaa gggccgttac aagggatgaa tttctttctt gctgccgata
     4981 aagggcggga aaagcgcgat ggcagtacgc tgggcgatcg cctgaattac tgggatgtga
     5041 aaatgagtat tcagtatgac tttatgctga agtaagaaat aacgccggag agaaaaacct
     5101 ccggcgtttc agattgttga caaagtgcgc gttttttatg ccggatgcgg cgtaaacgcc
     5161 ttatccagcc tacaaaaact cataaattca aagtgttgca ggaaaaggta ggcctgataa
     5221 gcgtagcgca tcaggcaatc tctggtttgt tttcagatga aaacgccgga gtgaaaattc
     5281 tccggcgttt tggccgtgaa ttactgctgc ggaattgccg gtacagccgg aacgttaaga
     5341 gctggcatcg caaacatgcc aacaaaatct tctaacgaca ttttctgccc atttaacgtt
     5401 atctgaccgt tagcatattg caggctggtg gtgatggtat tgtcctgcaa ggtggtcaga
     5461 cggaacatct gccccattgc tgatgcacct tcaacttgct gtttcgccag ttttttcgct
     5521 tgatcttcct gataaccttc cagcttcgct acctgagtca tcaactcagt tgccatatcc
     5581 accggaatgg tcagtttcgc atccagagat ttaaccgaac gatctacttc ctgcgccagc
     5641 gtttgcggcg cttctttagt cgttgccgga tctttcagga acagcgacag attcagcgca
     5701 ctttcaccct gactgttttt ccagcttagc ggcgcgatag taatcaccgg atcgcctttc
     5761 aacatcagcg gcagggcgct aaagaaggct tccgtcactt tctcctgata aagttcggga
     5821 ttgttggcaa tttctggctg tgccagcagc gcctgagttt gcgcgttata ttgctggcta
     5881 aactgatgcc aggcttcacc atcaatctgg ccgactttta aagtcagctt gccgctgccc
     5941 agatcctgat tctgtacctt caggctgttt agcgagtaat ccagttggct attgatcgtt
     6001 ttaccgtcat tgaccagatc cgatttaccg ctgatctcca tgccttccag cagtgccagt
     6061 tctttgcctt ccactgaaat ggtcattttt tccagtgaca gtttttggtt tcctacgcgc
     6121 tcaccaaaac ttcccagcgt gctggaaccg tcggttttca gattattaaa ggtcaactgc
     6181 actttctggt tgtattcgtt aactgcgtct atccgaccac tttgcgcctc tccggaaagg
     6241 gagatggctt tgccgtctct gtcagcattt aactggaact cgccgccgct aaaggcgact
     6301 ttttcatcct tttgctcgta attcagtgga ttgagcgaaa tatcggaact ggaatcaccg
     6361 ctgtaaccaa tgcgcgagtt aatctcaaaa ggcgtttcac cttttgccat atcaaacagt
     6421 ggtttgctta cttcgttatt aaccagcgtg gtttgaattg atgccatcga cgggatcagg
     6481 ttcagttttt taagctgggc aagcgggaag ggaccatgat caaccgattc gttgaagatg
     6541 acgctctgac cgcttttaat ccacggattt tctttcccgg caatgggttt caccaacagt
     6601 tgcaactggc tgctgaatac gccgcgatga tagttttgat aactcacttc caggttggat
     6661 tcaggagctg tcagtttgag ttgcgcgttc gcctgcgcga ccatgtcttc gagatgggtt
     6721 tcaatcttct tgcctgtata ccatgcgccg cctgtccaga ctacgcctag cgcaacaatg
     6781 acgcctaccg ctaccagcga tttattcata atgattatcc ataaaatgaa atcaggcgga
     6841 ctggccgcct gaaggtgtta taagccttta ataagcttag caagagatgt taattttttc
     6901 agtaagctct tacagcttgt tgtaaacacg cgctaaacgg ccgtggcctt tgacagtcac
     6961 cggtgattcg ttggcggcaa taaacgctga ttcacccggt ttaagctgta actgctgaga
     7021 acctttccac aacgttgcat cgccttcgac gcagaacaaa atggcggcac tctgctggct
     7081 gatggtggtt tctttatcac taaggtcatg cagcgagaag gcaaaatcat ccacaggaat
     7141 cgggaagtcc agttctgcac cttgtttcac cggctgggtc aacaactggt tagccggttt
     7201 ggcttcgaat ttcacattgg caaccagttc cggaatatca atgtatttag gcgtcagacc
     7261 tgcacgcagc acgttatcgg agttcgccat cacttccagc gccacgcctt gcaggtaagc
     7321 gtgcggtgtt tcagcgaaca ggaacatcgc ttcgccagga ttcaatttca ccacattcag
     7381 taacagcggg gagaacaggc cgctgtcttc cgggtaaaat tcagaaatta aacgaatcgt
     7441 ttgccacggt tcaccctgct gactgtcgag cgccgatttt aaaatcgcca gcgcgcggga
     7501 tttttcttca ccctgcatat tcaacaggtt ggcgaacagt tcgcttaaac gttcggcatg
     7561 tggctgttgt aaaaagtgag caatcgccgg atgtgcaccg gcgaccggct ggagtaggga
     7621 gacaatctcg gaaaattcac gaaacgcgtt catcgcaagg aaaggcgtca gcgcaaaaac
     7681 cagctccggc ttgtggttag gatctttata gttacgctcg gcggcatcca tcgggatacc
     7741 tgcggcattt tctttggcaa aaccgatttc agaattgtgt ttgtttggat gaacctgaat
     7801 ggagagtggc tgtgctgcgc ataatacttt gaacaggaaa ggcagttcgc caaagcgttt
     7861 ggcaacggcc tctccgagca gagtcgattt atcactctca atcacatcac gcagtgaaac
     7921 gatatctccg gcggcattct gcactcgtga actgcttttc ggatgtgcgc ccatccacag
     7981 ctcggccatc ggctggctgg acggattttc cataccataa agttcagtca acgccgtttt
     8041 gctaccccag gcatagtttt gcactgagtt aatgagtttt tgcatgatca atccctgttt
     8101 taatgtggaa attaatcccg ctattaaagc aagaatccta cgggaagtaa cctggagccg
     8161 caaaaagtcg tactagtctc agtttttgtt aaaaaactgt gtaggatatt gttactcgct
     8221 tttaacaggg caacggaaca cccgcccaga gcataaccaa accaggcagt aagtgagaga
     8281 acaatgtcaa acaaaccctt tcattatcag tctccttttc cactcaaaaa aaatgatact
     8341 gagtattacc tgctaaccag tgaacacgtt agcgtatctg aatttgaagg gcaggagatt
     8401 ttgaaagtcg cacccgaagc gttaactctg ttggcgcgtc aggcgtttca tgatgcgtcg
     8461 tttatgctgc gtccggctca ccaacaacag gtggccgaca ttctgcgtga cccggaggcc
     8521 agcgaaaatg ataaatatgt ggcgctgcaa ttcctgcgta actccgacat cgcggcgaaa
     8581 ggcgttctgc caacctgtca ggataccggc accgcgatta ttgttggtaa aaaagggcag
     8641 cgtgtatgga ccggtggtgg tgatgaagcg gcgctggcgc gcggtgtcta taacacttat
     8701 atcgaagata atctgcgcta ctcgcaaaac gcgccgctgg atatgtataa agaagtgaat
     8761 accggcacca atctgccagc gcagatcgat ctttatgccg ttgatggcga cgaatacaaa
     8821 ttcctctgta tcgcaaaagg tggtggttcg gcaaacaaga cgtatctcta tcaggaaacc
     8881 aaagcgttac tgacgccggg gaaactgaaa aattacctgg ttgagaagat gcgcacgctg
     8941 ggtaccgcgg cctgtcctcc gtatcatatt gcgttcgtta ttggtggaac ttctgcagaa
     9001 acgaacctta aaacggtgaa actggcttcc gcgaaatact atgatgaact gccaacggaa
     9061 gggaatgagc acggtcaggc gttccgcgat gtggaactgg aaaaagaatt actgatcgaa
     9121 gcgcaaaatc tcggtctggg tgcgcagttt ggtggtaaat acttcgctca cgacatccgc
     9181 gtgattcgcc tgccacgtca cggcgcatcc tgtccggttg gtatgggtgt ttcctgctct
     9241 gctgaccgta atatcaaagc gaagatcaac cgtcagggga tctggatcga aaaactggaa
     9301 cataatccag gtaaatatat ccctgaagag ctgcgtaaag cgggagaagg cgaagcggtg
     9361 cgcgttgacc ttaaccgtcc gatgaaagag atcctcgcac agttgtcgca gtatcccgtt
     9421 tctacacgct tatcgcttaa cggcacgatt atcgttggtc gtgatattgc tcacgccaaa
     9481 ctgaaagagc ggatggataa cggtgaaggg ctgccgcaat acattaaaga tcatccgatt
     9541 tactacgcgg gtccggccaa aacgccggaa ggttatgcct ccggttctct tggcccaacg
     9601 accgccggac ggatggattc ttatgtcgat caactgcaag cgcagggcgg aagtatgatc
     9661 atgctggcga aaggcaaccg cagccagcag gtgacggatg cctgtaaaaa acacggcggc
     9721 ttctaccttg gcagtatcgg tggtccggcc gctgtattgg cgcagggaag tattaagagc
     9781 ctggaatgtg ttgaatatcc ggaactggga atggaagcca tctggaaaat tgaagtggaa
     9841 gatttcccgg cgtttatcct tgtggatgat aaaggaaatg acttcttcca gcagatacaa
     9901 ctcacacagt gcactcgctg tgtgaaataa acagagccgc ccttcggggc ggttttttta
     9961 catggcacga aagaccaaac atttgttatc aaatagtaaa taatgagtga gctaaaagtt
    10021 gcttaacgaa agcaaaacag aaagaaaaaa ttaatcaggt gaggagcagg ccatgaatac
    10081 agtacgcagc gaaaaagatt cgatgggggc gattgatgtc ccggcagata agctgtgggg
    10141 cgcacaaact caacgctcgc tggagcattt ccgcatttcg acggagaaaa tgcccacctc
    10201 actgattcat gcgctggcgc taaccaagcg cgcagcggca aaagttaatg aagatttaga
    10261 cttgttgtct gaagagaaag cgagcgccat tcggcaggcg gcggatgaag tactggcagg
    10321 acagcatgac gacgaattcc cgctggctat ctggcagacc ggctccggca cgcaaagtaa
    10381 catgaacatg aacgaagtgc tggctaaccg ggccagtgaa ttactcggcg gtgtgcgcgg
    10441 gatggaacgt aaagttcacc ctaacgacga cgtgaacaaa agccaaagtt ccaacgatgt
    10501 ctttccgacg gcgatgcacg ttgcggcgct gctggcactg cgcaagcaac tcattccgca
    10561 gcttaaaacc ctgacacaga cgctgagtga aaaatcgcgt gcatttgccg atatcgtcaa
    10621 aatcggtcga acccacttgc aggacgccac gccgctaaca ctagggcagg agatttccgg
    10681 ctgggtagcg atgctcgagc ataatctcaa acatatcgaa tacagcctgc ctcacgtagc
    10741 ggaactggct cttggcggta cagcggtggg tactggacta aatacccatc cggagtatgc
    10801 gcgtcgcgta gcagatgaac tggcagtcat tacctgtgca ccgtttgtta ccgcgccgaa
    10861 caaatttgaa gcgcttgcga cctgtgatgc cctggttcag gcgcacggcg cgttgaaagg
    10921 gttggctgcg tcactgatga aaatcgccaa tgatgtccgc tggctggcct ctggcccgcg
    10981 ctgcggaatt ggtgaaatct caatcccgga aaatgagccg ggcagctcaa tcatgccggg
    11041 gaaagtgaac ccaacccagt gtgaagcatt aaccatgctc tgctgtcagg taatggggaa
    11101 cgacgtggcg atcaacatgg gtggcgcttc cggtaacttt gaactgaacg tcttccgtcc
    11161 gatggtgatc cataatttcc tgcaatcggt gcgcttgctg gcagatggca tggaaagttt
    11221 taacaaacac tgcgcagtgg gtattgaacc gaatcgtgag cgaatcaatc aattactcaa
    11281 tgaatcgctg atgctggtga ctgcgcttaa cacccacatt ggttatgaca aagccgcgga
    11341 gatcgccaaa aaagcgcata aagaagggct gaccttaaaa gctgcggccc ttgcgctggg
    11401 gtatcttagc gaagccgagt ttgacagctg ggtacggcca gaacagatgg tcggcagtat
    11461 gaaagccggg cgttaatctg caacatacag gtgcagccgt ggaatgatca aacgaagcgg
    11521 ctgcgcctga ggtttataac ggtgctgcac attgtcggca tcgtaattta acaactcacc
    11581 aacgtccggc acgcccgcgc cattatcccg attaatcagt gcaatcagtg gtgtagggca
    11641 ggcgtgttgg acttgttttt gatctccttt gtaccagacg cgggcaatcg gctgcacctt
    11701 cactggacgt ttgattttta acttcgcgtt ctgtggcagg gcagcgatat cctgatactc
    11761 tcgctccagt tttctttgcc actcctcgcg cgtccacggt gcgacactgc gtggtgattt
    11821 caggcttttt tccagctgtg ccaggacttc atcacgatgc aaattcttaa tgatatgttt
    11881 attagcccaa ccaaagcgta aagtggcggg gtcgtgcaga acggtgagcg tgcggtaagc
    11941 attaagggtg atcagccccg gcaaatgacg atgcacccat tcaaaacgtg ccgcggtggg
    12001 gagttctgat tcaaccgtga cgatatgctc gaacgtggtc ttgagtttat tgatgtgctg
    12061 aatatgactg accaacgctg cttgcgaaag gttatcgacc tggtaacaca acacgccagg
    12121 cagacggaca gcggccttgc tgctgcgatt ttcggactgt tgttgaataa ataaatggcg
    12181 gaaatgacgc aacgccagcg actgcgcgtc gttgccgaga tgttgtttta cctcaatacg
    12241 attaagcgga ttatgctcat cctctttttt tacctccggc aaagagaaca cgcgggcaac
    12301 caatagcttg tgttgctcaa gatgagcggc aaatgtagcc agctcttgtt ccatctggcg
    12361 aaaggtagtg ttgagtcggt ctacgagatc gtaacgcgcc ataatattaa ccactttagt
    12421 tacaacatac ttattttata gcacagtcgt ggtgacttga ccatcgtgcg cgtagtgtca
    12481 ggcagaggta aattgcggga tgttatgcca taacggccag ctaaacgaga agcgagcacc
    12541 acccagttcg ctgatgtcac aattaaccgt accgcccatt gtcagtgcta ttgagtggac
    12601 aattgccagc cccagcccgc agccgccggt tgagcgatcc cggctgggat cgaggcgaac
    12661 aaaaggttca aagatatgtt cgcggttttc tggggcaatc cctgggccat catcctcaac
    12721 aattaatgtc gctctattcc ccgacagtag caggctggtt tcaaccgttg aatggcagta
    12781 gcgcagggcg ttattgagca aattatccag cacgcgctcc attaagcgca tatccaacgc
    12841 cgcataatgg ccttgcatga gcgtttttat ccgtaccgtt ttatcgggcg ttactgcctg
    12901 aatatctgcc agatgcgttg acagccacaa cggcaggtct ggttcgctaa gatgaagctc
    12961 gttttgtggg cgatcgagtc gggcataagt cagcagctct tcaattaaag cttcaagttg
    13021 actgatatca cgattcaacg cctgggattc ggcggcgctc aggttatcgc tcatctccag
    13081 tcgataacgc aggcgcacta acggtgttcg cagttcgtga gcgataccgt caataagctg
    13141 ttttttgctg gcaattaagg cgttgatatt gtccgccatc tggttaaatg cgataccaag
    13201 tcgttcaaag ctcgaaccct catcaaagtg gatacgttca ttgagatgcc catcgccaaa
    13261 tcgttgcgcc gctgcttcca gttttaacat atcctgccag tgcggacgca tccagataaa
    13321 caccggaaag gcgagggaaa tagcaataaa agcgatcagg gcgatatcca gcaatcgcat
    13381 ttgatggagg taataaagat aaggaacagg accaactgcc agcacgtagt ggctgcgcgg
    13441 gatacgctgc aaaaacgtgt actgatcgtc cagggcgaca atttcgccgc cacgcagtcg
    13501 gtgcatggaa atatcatcaa gatggtattt actcagtggc tcgacacgca gatcgaaaga
    13561 gagatttaaa tccatctctt tcagcgtctt accccagtcg tgtgggggga tctctcgcaa
    13621 ttcgctgcgc atcagataca gcgaactgtt catcaaatca tccagcgact gtttgcccgc
    13681 gcgttcggcg gtaaatttgt acaccagccc aaccagcaga gacatcacaa ggaagcagac
    13741 aaacaataac aggtaaaact ggataaacaa ttttttcatt ggcatcgccc gcaacagaat
    13801 actattcgta ttaattcacg actgtggagc aatgtaaacc gccaactgga taagaccttg
    13861 tttggaagat aatgtccgga acagacggat gtatccccgg tgtattcata ttcagtaaat
    13921 tcaattttcc accggggctt gtttattatg ctaaggttta atttaacgtg acgctttgtt
    13981 gttctttagt ctctctatcc ttaagaagac gggctgatat tacaccggac attattgaac
    14041 cattcacatt aagcgctgtt cttgccatat caacgatcgg ctctatggca ataaaaataa
    14101 ctactaatgc gacagggaaa ttaagtgttg aaaggacaat cagcgccgcg aatgtcccac
    14161 cactacctac gcctgctacc cctattgagc caagcgcgat ggcaggtaaa agagttgcga
    14221 ggaattgcaa tgagaaggga tcgataccca ttgttggagc aatcatcgcc accatcacgg
    14281 caggataaat cccagcgcag ccattttgtc ccatgctgga accaaaagac gctgcaatat
    14341 tagcaatcgt atttggaacc cccaattttt ccaatgcaga aatagccaga ggaattgaag
    14401 ccgcactact gcgagaaaca aacgcaaagg taagtacttg ccagacggtc ttgtaatagt
    14461 taacggggtt tttacctatt aacagcaaaa tgcccgagtg aacaataaac atcacaaaaa
    14521 cggccagata acaggcgacg atgaacccga gcaaattggt aaactgtgaa agctgatacg
    14581 ttgagaagac tttcgccatt agtgccatta ccccataagg cgtcagcgca atgacaatac
    14641 ggaccatttt cattacccat atttgaatcg cgttgatccc cgcgctcaat ttttcgccct
    14701 ctgctggtgc atctttcttt acttttaaca gcgcaatacc agcaagaatt gtaaagatga
    14761 cgatgccaat aaccgaaaca cttcttgccc cggtcagatc aaggaagagg ttggttggga
    14821 tcaatgacgt aatgagtcgc ggtaaagata ccgcagctgt tttcgagacg tcatcgctgg
    14881 ttaaagagga ttgcatatga ccaaaggcgc tggcatcaag gccaattgca ctggcaatag
    14941 ccaggccaac taaacctgca atcatgacta aacacaacat acaggcaaca atagttaaag
    15001 acatcttgcc aataccagcg ctattttcga gcttattaat cgctgaaaga atcgaaatga
    15061 atatgagtgg aacggcaaca agttttaata aattaacata accgttacca gcaatattat
    15121 accattcgat aacggtttca ctgcttgtct caggaatgaa atgcagggag ataccaatga
    15181 tagcccccag gaaaatcgcg ataaaaacac ttttggaaag ggattcgctc ttttgatccg
    15241 ttttgaaaat cccccatgag cagatcaaaa aaagcacaat atatgtgaaa gttgtaaccg
    15301 ttttttaagc ctcttgcctc attcagcacc tgaaatgccg gaaagagttt gttgtaaaaa
    15361 atctgcgcca taagacgcag ggacgaggcg attatagaga aattttttct cattttcata
    15421 tcaaatgtag ggggtttgga atttgaataa gctcttatta attgaattcg ttaattgttc
    15481 tctttttcag atgaacaaat attagtcatc tgattgttta ttgtcactat cccatgcatg
    15541 aggcgcaaaa agatagcctt tgttacgcac agttttaatg cgataaggtt ctgcggcgtt
    15601 atcgagcagt ttttttctta accgcgaaat agccacgtcc acgctacgat ccagtccgtc
    15661 ataactgacg ccgcgtaaat ttttcagcaa tgcatcgcgg tccatgattt gcccggcatg
    15721 cgtagctagt tcccacaata attcgaaatc agctgtcgag agcgagattt cagtgttagc
    15781 cagggtgact acgcggttga tggggtcgat agttaacgtg ccgaaatgca gggctttgta
    15841 gggagtaaga gacgtttcct gaagaccttt ggtcagtgtg gcttgctcat tctgacgcaa
    15901 atgcaaacgt aaacgcgcta gcaaaacagc agggggcgtc gttttgagaa tatagtcgca
    15961 ggcacccatt tccagtgcca ggatgtggtt catatcgcta tcgagagagg ttagaagaac
    16021 aatcggtcca gaccactttg cgcgtaaatc acgacaaatg gtcatgccgt ccttgcctgg
    16081 tagcatgatg tcgagtaaca ccaaatccgg attttctcgc aaaatggttt cttcggcctg
    16141 gtcgccgcgc ggttctacgg taacctgcat atcatgtttt gccaggtacg cggcaatcag
    16201 tgaaccgact tccgcatcat cttccacaaa tacgatagtg ttcataacat tcacggtaga
    16261 tataaaaacg tcacaataca ccgcgctgtt tttacttact atcaatcgtt tctaaacaag
    16321 ttttcatcag gtattctact gatgacttgt tatggaagtg ttaaggtaaa aagatggggc
    16381 tggtaatcaa agccgcgctc ggtgcgctgg tagtgctgtt gattggtgtt ttagcaaaaa
    16441 cgaaaaatta ttatatcgcc gggctgattc cacttttccc gacctttgcg cttatcgcgc
    16501 attatattgt tgccagcgaa cgtggcattg aagccttacg cgcaaccatc atttttagta
    16561 tgtggtcgat tattccctat tttgtttacc tggtgtcgct gtggtatttc accggaatga
    16621 tgcgcttgcc cgccgctttt gttggttctg ttgcgtgttg ggggataagc gcatgggtgt
    16681 tgattatatg ctggataaag ctgcattaac gcagatgacg accgccatca agtgggaaac
    16741 tgcgtccggt gacaaagcaa ctggtaagta agtaatcgac caggtcgatc acttctttct
    16801 cgccaggcgc ggttttcatc agtgatttat tcagcgcctg ttgtcgatat tcggcatcat
    16861 catgttcatt aaacaggatc agcgatggcg caatagaatt cactttcact tccggtgcca
    16921 gcttgcgggc aaacgagcgg gtcatattat ccagtgccgc tttgcttgca gcatacgcaa
    16981 catgtttgtc gctaccgcgc tccaccacat aatcggtaaa gtgaatgata tcgctggcgg
    17041 cgtgtccgtg cccacgcagt aatctttcca gcgcatggtt gagcaggtat ggggtattaa
    17101 cgtggatctg catcatgcaa gccagtacgt cggccagtgg cgcacccggt ttttccgcaa
    17161 tccacgcact ggcgttatgc aaaatagcac gcagaccatg ggtggttttt agtacttcat
    17221 cggcaaacgc catcacaccg tcgttggtcg aaaaatcagc ctgaatacac tgcgcacctg
    17281 cattaatcag tccatcaatg gctggatagt gtgtccgata gctgacaatc accggttgct
    17341 tttgattaat gaaatgccat gcgagggcga ggccgatgcg acgacctccg ccagtaatta
    17401 atattggcaa gggctgggtt ttacccatcg ttatctcctt tgctatccaa cgatgggatg
    17461 aacgttatcc caccagcatc caggtcgccg gaactgaggc aattaacagc atgccaatca
    17521 gcaccatttc ctgacggttc agtacattat catgtgtatg cgttttgcgc gcatacagga
    17581 aaaccagcag ccccggtgca tacagaacaa cggataacag caagtgcatt ggaccagagg
    17641 cgtacaataa ccataagcca taaatgcagg caccgacacc taccgcttta tgcagtggac
    17701 gggttgcgat tttcagcaag aacgcgccga ccaggaaata gggcaccaga atcatttctg
    17761 aggcgatggt cagcaacgtg ttgtaatcgg aaccggtcag ccagatgagt accagacaaa
    17821 tttgcacgca gatgttggtc aaccacagtg aggcggatgg cgcagcttgt gcgttctggc
    17881 gcgcaaaaat gcgtgggaat gctttatgag tggcagccag gaacggaact tctgccgcca
    17941 tgatggtcca gcttaagtac gcgccgcaaa cggaaacgat caaaccggca gcgatgatga
    18001 tttcgcccca tggtcccatc atttccacca tcagaccggc cattgacggg ttacgaattt
    18061 cagccagttc aggacgtgcc accacaccca gcgaaagcag cgttaccagc aagtaaacgc
    18121 ccagagcgga gagaaccgcc agcagtgtcg ctttgccaac atcacgttta ttacgcgcac
    18181 gcgcagaaac aaccacggca ccttccacac caatgaaaac ccacagggtg atcagcatgg
    18241 tgtttttcac ctgttcccaa acaggtacgc caagtgcaag tccggtgaag tcgagcttga
    18301 aggtatccag tttgaacatc atcatcgcca gcacaacaaa cagacccagc ggcaacaatt
    18361 ttgccagtgt cgccaccagg ttaatgctgg cagcggtttg caccccgcgc aggatcaaaa
    18421 agtgaacaat ccacaataaa gccgatgcac cgactatcga ttgccaggta ttaccatcgc
    18481 caaacaggcg caattccggc gtgtccgtaa aaaagcttaa cgcggaaaaa acgatcacca
    18541 gataggagac gttggcgatg accgcgcaca gccagtatcc ccatgcggaa caaaagccga
    18601 ttagctcgcc aaacccttcg cgggcatagg taaagatacc gccatcaagt tcgggacgaa
    18661 tgcgcgtgag gatcagcatg gcaaaggcca gcaataaaat gccagcgcca gtaatacccc
    18721 agccgatgag cagtgctgcc gggctggcaa ctgccgccat attttgcggc agactgaaaa
    18781 cacccgcgcc cagcattgag cttaatacca gcgcggtgag tgcgctcagt cccagtttct
    18841 tttccatcga tatccagtga ttaaaacgtg ataagaagaa taaatattag ggcaaaacgc
    18901 ataaaaatgg agtttgccac tgaagcgcgg gattttacga agagatgtgt ctgcatgcaa
    18961 tggtcgcggg gaaatttgtg actaaaaatg cgaaaggcgg catcaagccg ccttatagga
    19021 gtaactgaat agttatttat acagatctgc gctgacggtc aggttattac cacgtttctg
    19081 ccactggcgg gtgatgtggt aatacttagc acctttcttc gcggcacgtt tcgcaacctg
    19141 ataggagact tcggtcatgt tgccgtagtt gccagagaat ttgatgctgt cgaacgggac
    19201 catcatcgct gctgtcgctt tgttcagttc ttcgacttta gtgccatccg ggagcgtgac
    19261 ggtgtaacgc ccgccttttg atgactgggt ttcaaagaag cgaccgactt cagaacttgg
    19321 tgatgcggta gtcgcaacac ccgggatctc aactttcttc gcggcttcgc caccggcagc
    19381 cagagctgca cgtcctgctt cggaatctgc cgggatcaca tccgggctct ggacgatacg
    19441 tttcttagca tcttttttat agatgaatgc agtaatacgc tggttgccgc cctggttggc
    19501 atcgatttga cgaacgatgt agaaagagta ggcacctttc gcttttgccg ctttggtgat
    19561 ggcatcattg acttctggct ggctacgata gaagccctgg acggtgaccg tgtcaaacgg
    19621 ttcaatcaga acagcctgat ctttcggcag ttcgacaaca ccgttaatta cgcgattact
    19681 tgtttcttct gctttttcag catcggcttt atagaggtca gcgaccacac gccagttacc
    19741 gctgttacca aaatcagaag tgtcgacaac ataaaaagag gcggcacctt ctttatctgc
    19801 gcgacgagaa acggctttca ccgcttcgcc aatagcatta aaacgaccgg taaccactac
    19861 acggtcaaaa ggtttaaccg ctgccgcttg ctccggtgtc agttctgttg ctgcgttaac
    19921 ggagaatgcc gtagcagaaa gcagtgccga cgccaggagg gtgttcttaa gcttcataaa
    19981 aataatcctt cgccttgcgc aaaccaggta ctggtattgt tattaacgag aaacgtggct
    20041 gattattgca tttaaacggt gtaactgtct gcgtcatttt tcatatcaca ttccttaaac
    20101 caattttaat cctgctcaaa tgaccgtcta tgcttaaaaa acagccgtat cagcatcatt
    20161 actactgaag caactgaatt gtataagtta atttaatgtt aagtagtgat tcgtgccggg
    20221 gcgatgtctc gttttacccg accgtcgaag acaattatca gtctttatcc ggcgttctaa
    20281 ggtgtttatc ccactatcac ggctgaatcg ttaatatttt gcgagttcac gccgaaatac
    20341 tgatttttgg cgctagatca caggcataat tttcagtacg ttatagggcg tttgttacta
    20401 atttatttta acggagtaac atttagctcg tacatgagca gcttgtgtgg ctcctgacac
    20461 aggcaaacca tcatcaataa aaccgatgga agggaatatc atgcgaattg gcataccaag
    20521 agaacggtta accaatgaaa cccgtgttgc agcaacgcca aaaacagtgg aacagctgct
    20581 gaaactgggt tttaccgtcg cggtagagag cggcgcgggt caactggcaa gttttgacga
    20641 taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg aatagcgtct ggcagtcaga
    20701 gatcattctg aaggtcaatg cgccgttaga tgatgaaatt gcgttactga atcccgggac
    20761 aacgctggtg agttttatct ggcctgcgca gaatccggaa ttaatgcaaa aacttgcgga
    20821 acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt atctcacgcg cacaatcgct
    20881 ggacgcacta agctcgatgg cgaatatcgc cggttatcgc gccattgttg aagcggcaca
    20941 tgaatttggg cgcttcttta ccgggcaaat cactgcggcc gggaaagtgc caccggcaaa
    21001 agtgatggtg attggtgccg gtgttgcagg tttggccgcc attggcgcag caaacagtct
    21061 cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg aaagaacaag ttcaaagtat
    21121 gggcgcggaa ttcctcgagc tggattttaa agaggaagcg ggcagcggcg atggctatgc
    21181 caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa ctctttgccg cccaggcaaa
    21241 agaggtcgat atcattgtca ccaccgcgct tattccaggc aaaccagcgc cgaagctaat
    21301 tacccgtgaa atggttgact ccatgaaggc gggcagtgtg attgtcgacc tggcagccca
    21361 aaacggtggc aactgtgaat acatcgtgcc gggtgaaatc ttcagtacgg aaaatggtgt
    21421 caaagtgatt ggttataccg atcttccggg ccgtctgccg acgcaatcct cacagcttta
    21481 cggtactaac ctcgttaatc tgctgaaact gttgtgcaaa gagaaagacg gcaatatcac
    21541 tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg atccgtgcgg gcgaaattac
    21601 ctggccggca ccgccgattc aggtatcagc tcagccgcag gcggcacaaa aagcggcacc
    21661 ggaagtgaaa actgaggaaa aatgtgcctg ctcaccgtgg cgtaaatacg cgttgatggc
    21721 gctggcaatc atcctgttcg gttggctgtc aagcgttgcg ccgaaagagt ttcttgggca
    21781 cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac gtggtgtgga atgtatcgca
    21841 cgcgctgcat acaccgttga tgtcggttac caacgcgatt tcagggatta ttgttgtcgg
    21901 agcactgttg cagattggcc agggaggctg ggttagcttc cttagtttta tcgcggtgct
    21961 tatagccagc attaatattt tcggtggctt caccgtgact cagcgcatgc tgaaaatgtt
    22021 ccgcaaaaat taaggggtaa catatgtctg gaggattagt tacagctgca tacattgttg
    22081 ccgcgatcct gtttatcttc agtctggccg gtctttcgaa acatgaaacg tctcgccagg
    22141 gtaacaactt cggtatcgcc gggatggcga ttgcgttaat cgcaaccatt tttggaccgg
    22201 atacgggtaa tgttggctgg atcttgctgg cgatggtcat tggtggggca attggtatcc
    22261 ggctggcgaa gaaagttgaa atgaccgaaa tgccagaact ggtggcgatc ctgcacagct
    22321 tcgtgggtct ggcggcagtg ctggttggct ttaacagtta tctgcatcat gacgcgggaa
    22381 tggcaccgat tctggtcaat attcacctga cggaagtgtt cctcggtatc ttcatcgggg
    22441 cggtaacgtt cacgggctcg gtggtggcgt tcggcaaact gtgtggcaag atttcgtcta
    22501 aaccgttgat gctgccaaac cgtcacaaaa tgaacctggc ggctctggtc gtttccttct
    22561 tgctgctgat tgtatttgtt cgcacggaca gcgtcggcct gcaagtgctg gcattgctga
    22621 taatgaccgc cattgcgctg gtattcggct ggcatttagt cgcctccatc ggtggtgcag
    22681 atatgccggt ggtggtgtcg atgctgaact cgtactccgg ctgggcggct gcggctgcgg
    22741 gctttatgct cagcaacgac ctgctgattg tgaccggtgc gctggtcggt tcttcggggg
    22801 ctatcctttc ttacattatg tgtaaggcga tgaaccgttc ctttatcagc gttattgcgg
    22861 gtggtttcgg caccgacggc tcttctactg gcgatgatca ggaagtgggt gagcaccgcg
    22921 aaatcaccgc agaagagaca gcggaactgc tgaaaaactc ccattcagtg atcattactc
    22981 cggggtacgg catggcagtc gcgcaggcgc aatatcctgt cgctgaaatt accgagaaac
    23041 tccgcgctcg tggtattaat gtgcgtttcg gtatccaccc ggttgcgggg cgtttgcctg
    23101 gacatatgaa cgtattgctg gctgaagcaa aagtaccgta tgacatcgtg ctggaaatgg
    23161 acgagatcaa cgatgacttt gctgataccg ataccgtact ggtgattggt gctaacgata
    23221 cggttaaccc ggcggcgcag gatgatccga agagtccgat tgctggtatg cctgtgctgg
    23281 aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc gatgaacact ggctatgctg
    23341 gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat gctgtttggt gacgccaaag
    23401 ccagcgtgga tgcaatcctg aaagctctgt aaccctgacg gcctctgcgg aggccgtcac
    23461 tctttattga gatcgcttaa cagaacggcg atgctttgac ctcccgctgt ttgttcaagc
    23521 gcaattttga caataattgt caacggcacg gaaagcagca tacccaccgg tcctaacaac
    23581 catccccaaa aaatcaacga caaaaatacc accaatgtcg aaagccccag cccacgtccc
    23641 atgatgcgcg gctccagaat attgccgaag accagattaa tcagcagata tcccgccagc
    23701 accagcaacg cttcgtagaa gccattaaac accagtacct gagcgatagg ggggattgcc
    23761 gcgagaactg aaccaatatt agggatgtaa ttaagcgcaa aggccagcaa tccccagaca
    23821 aaagcgaagc gaacatcgag tgcggcgagc atcgcccagg cgaccaggcc ggtgatgatg
    23881 ctgatggctg ttttcagcac cagataatga gaaacactgt caatcgcacg ttgaatcgcc
    23941 gccatccctt caaccggacg cgccatcatt tgctgaaatt ttccgggcaa ttgtggcact
    24001 tcgagcagca taaacagcac cgtcagcagc aataaaaata ttgatgacat ggcattagat
    24061 aactgcgtca ataagttggt gagcaacgtc atcgccgcgt tcggatcaat gtaatgcgcc
    24121 agttggtcaa ctgagacgtc aatccctacg cgttgcaaca acggctcaag agcttgcagc
    24181 ggcgtcataa tagagttgcg atattgcggt aacgtccgcg tcaactcgtt gagcgcggaa
    24241 cccagataag ctaatagcaa caccatcgcc atcacgatga ttgtcatcaa aatcgacact
    24301 gccagtacac gcggcacacg ccagcggacc atatgttgca ccagcgggtt aagaataaca
    24361 gcaataaata atgcgagaat aaacggcacg atgatctcgg cggcaaaacg gataccgcag
    24421 agaataatga ccagcattcc caacataatg acgattttta ggccattgag cgtgatgatc
    24481 ggctttgcca tgcttgtccc cgtttttctt cctttgctaa taataatgtc tttttcgctc
    24541 cgtcatcata acgaaaatca aaccgctcag gtaaagaagt gaaaatattt tgagttaatt
    24601 cttaagctat gatacaaatc aggcgtgttc aactaccgag gacaattatc atctgcgatg
    24661 acgagaagca acactgcgga taattgtaat attatggaca atttgttcag gacgttattt
    24721 tctacattta cccatcttcg tacctcatcc actatcttgc tcgtaggtga gcagcactgg
    24781 cgcaacgcgc tttagtcctt tcttctgcct aagcttgccc tatgcgcagg gcttgaccac
    24841 tttactttgg tttcgctgaa ttaagcgaaa attaaaatag ttctcttgca ggagaaggac
    24901 aatgtatatt tattggattt tattaggtct ggctattgct acagaaatta ccggtacgct
    24961 gtcaatgaaa tgggcgagcg tcagtgaggg aaatggcggc tttattttaa tgctggtgat
    25021 gatttctctg tcgtatatat ttctctcttt cgccgttaaa aaaatcgcct taggcgtagc
    25081 ttatgcgctg tgggaaggta tcggtatttt atttattacc ttgtttagcg ttttgttatt
    25141 cgacgaaagt ttatcgctga tgaaaattgc cgggttaacc accctggtcg ccgggatagt
    25201 gttgatcaaa tcaggtaccc gtaaagcgcg taaacctgaa ctggaggtga accatggcgc
    25261 agtttgaatg ggttcacgcc gcctggctgg cattggcaat cgtgctggaa atcgttgcta
    25321 acgtcttttt gaaattttct gacggctttc gtcgcaaaat atttggcttg ctctccctgg
    25381 cggcggtgct ggcggccttt agtgcgcttt ctcaggccgt taaagggatc gacttgtctg
    25441 tcgtttatgc attgtggggc gggtttggta ttgccgccac gttagccgca ggttggatct
    25501 tgtttggtca acggttaaat cgtaaaggct ggattggcct ggtcttgctg ttggctggaa
    25561 tgatcatggt gaaacttgcc tgatgaagac gctgcccgcg ctgtcgggca gcgtttgaac
    25621 attatttttg cgacagttga tccagcttgt cgcgaaaacc ggtaacagaa atggcccggt
    25681 tatcggcgcg ccagcgatct ttcgcggcag gagccgaact ttgcacccca attaattgcc
    25741 agccgtcatc ggtatgcaac atcagaggcg aaccgctgtc acccggcaag gtatcgcact
    25801 gatgtgacat caccgacgtt tgcgcccagc cagtcacttc acagttttga tgactgtata
    25861 acgtatcgag atgatcttca gggtatcccg cctgagtcac tttacgacca gccgatttta
    25921 atgcggcagt aagcgcggct ttatctccct caaataacgg caacggtgta atgccagaag
    25981 ggggattacg tagcacaatc aatccgaagt cccacggtgc agctgcggga ggaacaatcc
    26041 aaccatcccc atctgctttt aaccgctttc ccagtgtcgg atcaacgcgg ccttctatgt
    26101 cgtggatctc atagcgccaa agacctttat ttgacacaaa acgcagcgct accgccttat
    26161 ccgctttacc cttaggcggc gtcaataaac agtgtcctgc cgttaatgcc agattgggtg
    26221 caatcagcgt cgccgtacat aaattgccgc tggccgtttc cagttgccca accgcatccc
    26281 acggtgattg ggtcgtgtca ttcactggca cacgatcatc atgaccaaaa aacagggtgc
    26341 tgacctcatc gtttgccgat ttggcaacgt ctggtttatc tgcaaacaca aaagcagacg
    26401 tcaaactaat tgcacccaac actacagcaa tggttgtacg catatcacac tctggtgggt
    26461 aattatgatt attaaaagca aaccctcata aatactatag acgggacggg atgaaagtgg
    26521 gagtaaaatc agataactac agtcaggaaa gataaaaacg ggcggcaatc agtgcgcata
    26581 aaataaggat gataagaatg aactcaaagc gataacggcc caccatacta ccctccgcta
    26641 aaggcggtgc taaacgttac ccgcttagca ccgtaatttt caggcgcgag ggggcgcgcc
    26701 agcattactg ttgaaaactt acgctgcggg ttgtgcagca ggttttgccg gttgctgatg
    26761 gccgtgtttc ccggcgtgtt tcttcgctgc ctgcgctttt tgttcagggg ctttctgttc
    26821 agctttcgta tttttatgat gctttttagc cgcctgcgct ttctgggcag gtgctgcttt
    26881 atgctgtttt ttatgatgtg tagttttcgc tggcgctgct ttggtggtcg tcgcagtcgg
    26941 agcaggtgtg gtcgcagtct ctgcagcaaa ggcggcagaa gacagaccca tagcagcggc
    27001 aacaaccaga gctaatactt ttttcattgt cataccctca atttgttttt tcatttaacc
    27061 ccactgcggg gccgttgaaa taactatatc cctgggaatg gcagacttcc gtgagtggtt
    27121 ggtttcagcg tgtaacgata tgtacaaacg ctgaataaat tacagcgttg ataataacgt
    27181 attgtgactt aaggaaaatt tagctaccaa taatagtagt cttgatcggg ttaactatta
    27241 tactacttac aaagcggtag aataactgcg catcagtaat aaatgacaca cagcaaaatg
    27301 aatccgttta tttgggtact tataatcctg atgacgctag atgcgctgcg ggaattggct
    27361 ggcgcttcgt ctattttagg atggctatta acgttggttt gagcaggcaa taagtccgga
    27421 cgggtattta ccgcagtccg gacttatttt tcaggcgtgc agacgacgat gcaaacgcgt
    27481 cccgaccagc agagcaatga ccagcatcag agcaataaat gcgccgacgc cgttccagcc
    27541 atagttatgc cagaaaacac cacccagcgt cccggcaata ctcgacccca gatagtaact
    27601 gaacagatac agcgaggagg cctggccttt agcgcgtttt gcgcgggggc cgatccagct
    27661 gctggctact gagtgggctg cgaagaatcc ggctgagaag agtaacattc cggcaaagat
    27721 cagccacagc gagctaaata aggtcatcag taaaccaaac ggcataactc ccgtcgaaaa
    27781 caacatcacc ggaccgcgcc catagcgggt ggtcatggtt ccggctttgg gtgagctcca
    27841 tgtaccggtc aaataagcca gcgataataa gccaaccacg gcctgactga catgctaggg
    27901 ggaaagcatc aaccgatagc cgatgtaatt aaacagcgtg acgaacgacc ccatcagcaa
    27961 aaagccttct gcgaacaata acggtaatcc ccggtcacgc cagtgcagac gaaagttgat
    28021 aaataacgtc ttagggcgca gcgaagtcgg gcaaaaatgg cgtgattcag ggaggatttt
    28081 ccagaacatc aacgccgagg ccagcgcgaa acaaccgatt gccgccagag caattcgcca
    28141 gttgaaaaag tccgtgaaga caccactaat taagcgtccg ctcatgccgc caattgagtt
    28201 gccgctgata tacaacccca tcgaaaaggc cacgaaactg gggtggattt cctcgctaag
    28261 ataagtcatg ccaacagctg ccacgccact taacgaaagc ccaatcaagg cgcgcataat
    28321 caaaatgccg tgccagctgg tcatcattgt cgaaagtaac gtacaaatgg aggccaacag
    28381 tagcgccgtg accatcactg gtttgcgacc aatggcatcg gatagcgggc cagtaaatag
    28441 caaaccgata gccaacatcg ccgtggaaat ggacagtgaa atactactgt tcgcgggcgt
    28501 taagccaaac tcctgcgaaa gcaccggaag gataggctgc acacaataga gaagtgcaaa
    28561 ggttgccagt ccggcagaga acagcgccag ggtgacgcgc ataaattgcg gcgtaccgcg
    28621 tttaataaat tgatttggct gagaaatgct ttgcttgtca gtgtcgcttg ccggagcgcc
    28681 atcaacagtt gtagtacggc tcacttgaaa tccttgctaa atatgcctgt ggatcaggct
    28741 tatacatagg gtaggaaaat cgaattgttc tgtctaatat attaataatc tcaaataaga
    28801 tgttttaaat atgaatattg aacttcgtca tctgcgttac tttgttgctg ttgcggaaga
    28861 gctgcatttt gggcgcgcgg ctgcccgcct gaatatttcg caaccgccgc taagtcagca
    28921 gattcaggca ctggagcaac aaatcggtgc ccgactgctg gcacgaacta atcgcagtgt
    28981 attgctgacg gcagcaggaa aacagtttct tgcagatagt cggcaaatcc tgtctatggt
    29041 ggatgacgct gccgctcgcg ctgaaagact gcatcagggt gaagcggggg agttgcgtat
    29101 tggttttact tcgtccgctc cttttattcg ggcggtgtcc gatacgttat cgctgtttcg
    29161 ccgtgattat cctgatgtcc atttacaaac ccgcgaaatg aacactcgcg agcaaatcgc
    29221 tccgctcatt gaaggaacgc tggatatggg attgctgcgt aacacagcgt taccggagac
    29281 gcttgaacac gcagtcatcg tccatgaacc gcttatggcg atgatcccgt acgatcatcc
    29341 cctggcaaat aatccgagtg taacactggc tgaactggct gaactggctg aactggctga
    29401 actggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg ggactgggct
    29461 gtatgacgat attctcggac tgatgcgacg ttaccatttg acgcccgtca tcactcagga
    29521 ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg tttcaatttt
    29581 gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga ttgctgaaga
    29641 ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa gtccagctgc
    29701 gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc agcgaaaaag
    29761 cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac gtattgaagt
    29821 gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta tgcggtggtt
    29881 gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg cgcggtttat
    29941 cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct ggcgcagctg
    30001 gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct ggtgcgagag
    30061 ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt ggttgaaact
    30121 gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct tgctctgcgc
    30181 gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa agatgacttg
    30241 ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca ccagaaaaaa
    30301 cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac ggaaaatggt
    30361 attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg cgaggcgctg
    30421 gagcagcata ccggcgtacc ggtttatatt cagcatgata tcagcgcatg gacgatggca
    30481 gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt tatcgatcac
    30541 aacgtggggg tgggcgtcat taccgatggt catctgctac acgcaggcag cagtagtctc
    30601 gtggaaatag gccacacaca ggtcgaccca tatggtaaac gctgttattg cgggaaccac
    30661 ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca gctgcgtctc
    30721 aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc attgtgtcag
    30781 gcggcattgc gcggcgatct actggcaaaa gacatcatta tcggggtggg cgcgcatgta
    30841 gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact gattggctca
    30901 ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagacagcat ccgtcagcag
    30961 gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc taaccagggc
    31021 acgatggcag gcgctgcact ggtaaaagac gcgatgtata acggttcttt gttgattcgt
    31081 ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc tcaatttggg
    31141 ccaggaaagc ataacttaga ctttcaaggt taattatttt cctagtttat atttgtgaag
    31201 cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag acacttctgt
    31261 agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg gaaaaagggt
    31321 tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc tgcgtaataa
    31381 agatgccttg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag cagttaatcc
    31441 tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca attacaccct
    31501 catttcaaac ggcctggcaa acctgaccga taaagtcgat catgtcgtgg tagaagggac
    31561 tggcggctgg cgcagtctga tgaatgattt gcgtccactc tctgaatggg tagtgcagga
    31621 acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc atgcactgct
    31681 aacagctcag gcgatcgcca acgacgggct gccgctcatt ggctgggtgg ctaaccgaat
    31741 caacccagga ctggcgcatt atgcggaaat cattgatgtg ctgggtaaaa aacttccggc
    31801 accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac tggggcaata
    31861 catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg tgtaacgtct
    31921 gcgaaattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc tgatactccc
    31981 cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt gccgccaaca
    32041 gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca gggaaccata
    32101 atcccaggct acgaccatac aacatgccaa tggcaagacc gataaatagc gtcggtgtaa
    32161 agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt ttacaaagga
    32221 agatcccggc aatgatcatt aacagtggtg gggcggttaa aaaggattgt acggtgctat
    32281 agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc aacccgccta
    32341 gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga caggcgttca
    32401 ttaacgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg ataatcagcg
    32461 catagtcacg agcctgaacc gtcactgaga gttgtacgtt gtagagtaac gcgtcgctat
    32521 gattaatcag attgctaacc agccatgcca cgatggcgga aataatcacc gggccgagag
    32581 aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca gcaagcgggg
    32641 cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat ttccattcct
    32701 ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa agaatcatcg
    32761 caccttcgcg accaattgca ctgccgctgg ttactaccag cagcgaggca agcgatttaa
    32821 ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg taatcggtcg
    32881 gcgcatgagg gcgttgttgg gtaaatttct gccagcccat cagcaacaaa cccgccgcca
    32941 gtccgccgag cgccggagtt agcagccgtc gccaggagga aaggtttgtc gctgcattga
    33001 ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc gcatgacgaa
    33061 acccggcaac ggcaaagaca gcgagaatac cgacgactgt tgcgataagc agacggcgga
    33121 acatagtgcg cagatccgga tagatgtgta gatgatgcat gagcagaaat gggcaatgaa
    33181 acacgggcga ctattttgcg gcaaaagagg ccaaccgcaa aagcagtgcg tgctttaaga
    33241 gataaaaaaa cgtgacattg taacgtatta ttgccgaatc gcgcgtaatc gtcaccatcc
    33301 ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa tcaacagttg
    33361 tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt agaaagggtg
    33421 ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa ggtgccatgc
    33481 cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag ccatcaacag
    33541 cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa tgcctttctc
    33601 gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt cgcgatccag
    33661 ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca aacgctgcca
    33721 ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg gcgctaatga
    33781 cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg caaccagagg
    33841 tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc gcgccgcgac
    33901 agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat gtgcgggttg
    33961 ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca ggccataaaa
    34021 gagcacacgc ccgatcatct caccaacaag tatgaggatt agccccagaa caagtccggc
    34081 aacgtgaggt tcacggcgac gaatgagcgg acaaagccag catccaagac ctgcgcataa
    34141 caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc tggcttgttg
    34201 cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcacct cggcacaagc
    34261 gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa cgcgggctgc
    34321 ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga agaaagccag
    34381 cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt tgatacacgc aggtcatcat
    34441 ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc ccaacgcttg
    34501 cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa aaaatatgga
    34561 accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg agttaaacgc
    34621 tcgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca tcaacagcca
    34681 gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcatttttgg cggcgaacca
    34741 gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg taaagataac
    34801 cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta gccagataac
    34861 cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata ttgggttttg
    34921 tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc tggcgcagct
    34981 cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacgcat atgggttgtt
    35041 tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct ttttcagcat
    35101 tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta gcggcagccg atacaaacat
    35161 cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg gggcagactt
    35221 ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa gcgaacacgt
    35281 tctggtgcca gatgccatta tcttcctgcc agtcgccgcc agcatattca taaatacggc
    35341 ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg caggttttac
    35401 agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg ggctactcct
    35461 taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc cagcggtgaa
    35521 gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc accaaacata
    35581 tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat acgcggagtc
    35641 acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt atcgccatga
    35701 cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg gcaggcctgc
    35761 tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttggtttg gcgacttcac
    35821 tcagcatcca gtagatgggt ttgcgttcaa attttgctga ggttgcgggt tgggcgagga
    35881 tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc tctgttggca
    35941 tcaggtcagg caacaggata tcgcaatact tagccgaggc cgtcatgaag tggtcaatgc
    36001 caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga ttgatgtcgc
    36061 catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg gggacatcca
    36121 gtttttcttt tccacgaaca ccatcacggg tcgcggtcat tttcgtacca tggtcgatgg
    36181 catctgtcca tgtaaaaacg gaaatctgcg ttttaacagg attctcgagc atcgggaacc
    36241 attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg tttatgccga
    36301 cgttaccggt aagaacggaa agcatggcaa tagcgcggga cgtttgttcg ccgttggagt
    36361 gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg atctctcgtg
    36421 ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat tccggcgttt
    36481 tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg ttacgtggtg
    36541 cgttggcggg cagcgttttt tcatcgtaac caacacaata tttgtcgaga aatggctgat
    36601 cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg ccatcggtgc
    36661 cagggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta tagcgtggat
    36721 cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag taagtgacac
    36781 caccgccgct catccgcgtt tctgccgggt tatttccgaa cataacgacc agtttcgtat
    36841 tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc attgcggcac
    36901 tgatctgtgc ggtactgtag ctgccatagc gactgagaaa acccccgcaa gagttcatca
    36961 gacggtacgg gacgttcgag ttggtgatat ttccgccatc tacgcctgtt ccgtacagga
    37021 catgtacagc ctcattgccg taatctttca ggatccgccg aagattatca ctgatggtat
    37081 ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgctcg ccgacgcgct
    37141 tcatgggata tttcaacctg tcaggatgat tcatccgtcg gcggatagag cgcccgcgta
    37201 aacatgcccg aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca gactccaccc
    37261 agtacacggt gtcatctttc acatgcaaac gtagcagaca gcggctcccg cagttaacgg
    37321 tgcaggaact ccagaccgtt ttctcttcta ccggagcctc tgccgcccgg accatttggg
    37381 aaaatggcag agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg gaggttttca
    37441 tcagacttct atggctgatt tcagccttca tgagcgcctc tgtggtatgg attttcatca
    37501 ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt gtagtgggtt
    37561 atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc agtggtgaag
    37621 ggcgcagagt ggttagcgta ttcacacatc cgccatggtc gattttatcg ccagacatat
    37681 tgggctcgtg ccaggctccc tggcccatag cgctaactcc agggagaatg cgtggtgtta
    37741 ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata tcgccgttgg
    37801 caatcccacg tttctgtgca tctatagggt tgatccacac ctcctgacgg caggctgcct
    37861 tcaggacatc aatgttgccg taggtcgagt gagtacggga tttgtaatgg aaaccaaaca
    37921 gttgcagtgg gaaggttcta cgttcagggg agtcccagcc ttcaaaggtt gaggcataaa
    37981 ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca atttccgcca
    38041 gcttgctgga ataaatttca atcttaccgg aaggcgtttt aagtggattt gcctcggggt
    38101 cgtcacgaaa tgctttgtag gcgacaaaat ggccattggg atctttacgc ttatagatac
    38161 ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc attttggcgt
    38221 acagatgttg taaccattgt tcctgcgtgc gaccttctgt gaacttttga tagacgtcag
    38281 gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt tcgaattttt
    38341 cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag tcgttaggaa
//
LOCUS       DAJTPG010000029        37358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTPG010000029 DAJTPG010000000
VERSION     DAJTPG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(15..899)
                     /gene="yhdJ"
                     /locus_tag="OO230_001562"
     CDS             complement(15..899)
                     /gene="yhdJ"
                     /locus_tag="OO230_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5691371.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(985..1281)
                     /gene="fis"
                     /locus_tag="OO230_001563"
     CDS             complement(985..1281)
                     /gene="fis"
                     /locus_tag="OO230_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5691372.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1307..2272)
                     /gene="dusB"
                     /locus_tag="OO230_001564"
     CDS             complement(1307..2272)
                     /gene="dusB"
                     /locus_tag="OO230_001564"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5691373.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2601..3482)
                     /gene="prmA"
                     /locus_tag="OO230_001565"
     CDS             complement(2601..3482)
                     /gene="prmA"
                     /locus_tag="OO230_001565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5691374.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3494..4945)
                     /gene="panF"
                     /locus_tag="OO230_001566"
     CDS             complement(3494..4945)
                     /gene="panF"
                     /locus_tag="OO230_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5691375.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4935..5177)
                     /locus_tag="OO230_001567"
     CDS             complement(4935..5177)
                     /locus_tag="OO230_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5691376.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5286..6635)
                     /gene="accC"
                     /locus_tag="OO230_001568"
     CDS             complement(5286..6635)
                     /gene="accC"
                     /locus_tag="OO230_001568"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5691377.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6646..7116)
                     /gene="accB"
                     /locus_tag="OO230_001569"
                     /gene_synonym="fabE"
     CDS             complement(6646..7116)
                     /gene="accB"
                     /locus_tag="OO230_001569"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5691378.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8094..9068)
                     /locus_tag="OO230_001570"
     CDS             complement(8094..9068)
                     /locus_tag="OO230_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5691379.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9220..11160
                     /gene="csrD"
                     /locus_tag="OO230_001571"
     CDS             9220..11160
                     /gene="csrD"
                     /locus_tag="OO230_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5691380.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11465..12508
                     /gene="mreB"
                     /locus_tag="OO230_001572"
     CDS             11465..12508
                     /gene="mreB"
                     /locus_tag="OO230_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5691381.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12574..13677
                     /gene="mreC"
                     /locus_tag="OO230_001573"
     CDS             12574..13677
                     /gene="mreC"
                     /locus_tag="OO230_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5691382.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13677..14165
                     /gene="mreD"
                     /locus_tag="OO230_001574"
     CDS             13677..14165
                     /gene="mreD"
                     /locus_tag="OO230_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5691383.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14174..14767
                     /gene="yhdE"
                     /locus_tag="OO230_001575"
     CDS             14174..14767
                     /gene="yhdE"
                     /locus_tag="OO230_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5691384.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14757..16226
                     /gene="rng"
                     /locus_tag="OO230_001576"
     CDS             14757..16226
                     /gene="rng"
                     /locus_tag="OO230_001576"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5691385.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16294..20094
                     /gene="yhdP"
                     /locus_tag="OO230_001577"
     CDS             16294..20094
                     /gene="yhdP"
                     /locus_tag="OO230_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5691386.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20250..21695
                     /gene="tldD"
                     /locus_tag="OO230_001578"
     CDS             20250..21695
                     /gene="tldD"
                     /locus_tag="OO230_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5691387.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21823..22752)
                     /gene="aaeR"
                     /locus_tag="OO230_001579"
     CDS             complement(21823..22752)
                     /gene="aaeR"
                     /locus_tag="OO230_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5691388.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22935..23138
                     /locus_tag="OO230_001580"
     CDS             22935..23138
                     /locus_tag="OO230_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5691389.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23146..24078
                     /gene="aaeA"
                     /locus_tag="OO230_001581"
     CDS             23146..24078
                     /gene="aaeA"
                     /locus_tag="OO230_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5691390.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24084..26051
                     /gene="aaeB"
                     /locus_tag="OO230_001582"
     CDS             24084..26051
                     /gene="aaeB"
                     /locus_tag="OO230_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5691391.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26143..26415
                     /locus_tag="OO230_001583"
     CDS             26143..26415
                     /locus_tag="OO230_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5691392.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26471..26734)
                     /gene="yhcN"
                     /locus_tag="OO230_001584"
     CDS             complement(26471..26734)
                     /gene="yhcN"
                     /locus_tag="OO230_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5691393.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27099..27569)
                     /gene="argR"
                     /locus_tag="OO230_001585"
     CDS             complement(27099..27569)
                     /gene="argR"
                     /locus_tag="OO230_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5691394.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28004..28942
                     /gene="mdh"
                     /locus_tag="OO230_001586"
     CDS             28004..28942
                     /gene="mdh"
                     /locus_tag="OO230_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5691395.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29004..30071)
                     /gene="degS"
                     /locus_tag="OO230_001587"
     CDS             complement(29004..30071)
                     /gene="degS"
                     /locus_tag="OO230_001587"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5691396.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30161..31508)
                     /gene="degQ"
                     /locus_tag="OO230_001588"
                     /pseudo
     CDS             complement(30161..31508)
                     /gene="degQ"
                     /locus_tag="OO230_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31662..32060)
                     /locus_tag="OO230_001589"
     CDS             complement(31662..32060)
                     /locus_tag="OO230_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5691397.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32254..33381
                     /gene="zapE"
                     /locus_tag="OO230_001590"
     CDS             32254..33381
                     /gene="zapE"
                     /locus_tag="OO230_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5691398.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33600..34028
                     /gene="rplM"
                     /locus_tag="OO230_001591"
     CDS             33600..34028
                     /gene="rplM"
                     /locus_tag="OO230_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5691399.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34044..34436
                     /gene="rpsI"
                     /locus_tag="OO230_001592"
     CDS             34044..34436
                     /gene="rpsI"
                     /locus_tag="OO230_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5691400.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34831..35469
                     /gene="sspA"
                     /locus_tag="OO230_001593"
     CDS             34831..35469
                     /gene="sspA"
                     /locus_tag="OO230_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5691401.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35475..35972
                     /gene="sspB"
                     /locus_tag="OO230_001594"
     CDS             35475..35972
                     /gene="sspB"
                     /locus_tag="OO230_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5691402.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36015..>37358)
                     /gene="dcuC"
                     /locus_tag="OO230_001595"
     CDS             complement(36015..>37358)
                     /gene="dcuC"
                     /locus_tag="OO230_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5691403.1"
                     /translation="IVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGVLDPAK
                     SSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLLSRPLKLI
                     RSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMS
                     IEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNI
                     NHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVVMLLSLTVT
                     MFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRG
                     AEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVPAVVMIMPM
                     HFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9022 a         9361 c         9852 g         9123 t
ORIGIN      
        1 ggcttacact tcctttactt tgcaatgaga tcggggtcaa cttcgcttaa ccggcttcgt
       61 tttgacaggt tgccggtctt tctttttttc actttcgcca gttcttccgc ggagtaatgc
      121 gacgcgacat ccagccgtcg aagccccatt ttgatgtact cgctgttgat ctcaatacca
      181 atgaattttc gtccgctggc gatggctacg gcaccggtag taaagctacc ggcaaacggg
      241 tcgagaacga tatcgcctgg gttggaagag gcgagaataa tgcgtttcag taaggctttc
      301 ggtttttgcg tcgggtggtt ctcatattca tccatcaaat aacgcacgcg cggaaaatcc
      361 cagacgttac ccggtacttt ttgatgattg tatggctgtg gaggattttt gcgataatcg
      421 atcaacgcgc gctgcgatcc ggttttggct tctaccagaa tagcatcacc gttgaatgtg
      481 tagttctttg cgtctttcac catcatcagg atgggttcgt acatggagcc gtagtgtttt
      541 ttcgcctgta ctccagaact gtcatatgac cagacgatgc gacttttgat ggtaaaaagc
      601 ttgcggcact ggagatcgat aaagggcatg ttttccgtac tgttcataat gtacatgctg
      661 ccctgctttt tcagaacgcg gtggcactct gcaatcactt caaacagcca gtcgataaac
      721 agatcttctt tccaggcttc gatcagacca tcaaaatttt taccgatgtt atacggtggg
      781 tcggcaaaga gcagatcgac gctttcagta ggtagctttt taagttcggc aaaggcatca
      841 ccgtgaataa tggtcttagc ttcattacca aaccgggtcg gttcacatcc tgttctcatg
      901 gtcactccct ttgtgacacc tataaaaaag gcgcttcccc atgccgagta gcgccttttt
      961 aatcaagcat ttagctaacc tgaattagtt catgccgtat tttttcaatt ttttacgcag
     1021 cgtaccacgg ttgatgccca tcatcagcgc agcacgggtc tggttaccac gggtgtattg
     1081 catcaccatg tccaacaggg gctgttctac ttcagccagt accagctcat agaggtcatt
     1141 cacatcctga ccattcagtt gagcaaaata gttcttcagt gcctgtttaa ccgagtcacg
     1201 caggggtttt tgggttacct gatcctgaga gttaacggta gaaacggtca gtacgtcaga
     1261 atttacgcgt tgttcgaaca tagttctgtc agctctttat ttctgtttac gcaaaatttt
     1321 cgaagtatgc ctccaacgcc tccagctgtt cgctggcatc ctcaatggcg ttgaatgtgc
     1381 gccgaaactg gtcatttgga gcgtgttcct ggagatacca ggaaacgtgt ttacgtgcaa
     1441 ttcggtaccc ttttgccgga ccataaaagt catgcagttc ccgaacgtgc gcgcaaagca
     1501 agcgcttaac ctctgccaaa ggcagtgggg gcagcaactc cccagtgtcc agataatgct
     1561 ggatttcccg aaagatccag ggtcttccct gagctgcgcg gcctatcatc agggcatccg
     1621 cccctgtata gtcgagcaca gctctggctt taagcgggtc agtaatgtcg ccattcgcga
     1681 taaccggaat ggaaactttc tgcttaactg cccgaatact gtcgtactca gcttctccat
     1741 tgaacaaaca ggcgcgtgta cggccatgaa tggtcagagc ctgaatgcca cagtcttcag
     1801 ccagttgggc aatctcttcg cagttacggt gttccggtgc ccagccggtg cgaatcttca
     1861 gggtaacagg aacgtccact gcattgacga cctcggtaag gatcgattta acgacatccg
     1921 ggtactgcaa gagggctgaa cctgcgagct tgcgattcac ttttttagcc gggcaaccca
     1981 tattgatatc aataatctgg gcaccgcttt ccacgttaat acgtgctgca tctgccattt
     2041 ctttcggagc gctaccagca atttgcacgg tgcgaatacc gggttcatca atgtgcacca
     2101 tccgtaaacg agatttgtcg ctttcccaaa cctgtgggtt agaagacatc atctcggata
     2161 ctgtcaatcc ggctcccatc tcgtagcaca acgtccgaaa aggtctgtct gtaatgccag
     2221 ccatgggcgc tgcgatcagg cgatttctga gctgatattg tccgatgcgc atgagttaag
     2281 aaatgaccat actgtgactg caaggcggcg tatattacgc attttttgca cgagatgaaa
     2341 ggccaaactt tgaccaatcc tctgagatgg atcaaagaat tgcatttaaa atgagcgtgg
     2401 tgcgataatt actcataaaa atcatcatat tagaaaatag tgactaaaat ttacactcaa
     2461 agaaatttga gtaagttctc aatttttctt tatgaatgaa aattttggca cgcaaatttg
     2521 cgtaaataat cggcaaattt acgtgccttt tgtgagcttg ctcgcacttc gccccgcgtc
     2581 accctacggc gatgcgaagg tcaattctta cgaccggtaa tacggcacca ctcttctttt
     2641 tccacgaccg ggtccagtgc gaagctatcg gcataagctt cacaaacgct ctctgcctgg
     2701 cttgccagaa taccggaaag gcccagcaaa ccgcctgaaa ctggcaggac gctgattaac
     2761 ggtgccagtt cacgtaatgg gcctgcaagg atgttagcga ccaccacgtc ggctttcatt
     2821 tcttctggct gatctttcgg taagtagagt tccagacggt cagaaacgcc attacgttcg
     2881 gcgttatcgc ggctggcctg aatcgcctgc ggatcgatat caataccaat ggcttttgct
     2941 gcacccagtt tcagcgccgc gatcgccaga atgccggaac cacagccaaa gtcgatgact
     3001 gttttaccgg ttaaatcgag gctgtcgagc cattgcaggc acagagaggt ggttggatgg
     3061 gtacccgtac caaacgccag ccctggatct aacatcacgt tgacggcgtt ttcgtccggc
     3121 acatcacgcc agctagggca gatccacagt cgttcaccaa agcgcatcgg gtggaaatta
     3181 tccatccatt cgcgctccca gtctttatct tctagttgtt cgattttatg cgcgaagcct
     3241 gcgccgagca gcggatggtt ttccagaatc gccaccacgt cgttcatatc ggtttcagcg
     3301 tcgaacagac caatcacatc ggtgtcgccc cacaggcgcg tttcgcccgg cagcggctca
     3361 aatactggcg tatcgtgggt atcctgaaaa gtgatagaaa cggcacccgc ttccatcagc
     3421 gcatcgctaa gatcttccgc gttcgcgccg gtggtgttca gtttcagttg gatccaaggc
     3481 atggcaaaac tctttattta tcagtagtca aaacggtagc ttgcgggacg gatgtaccga
     3541 aacggtttcc gaccaggaaa gccagcaaac ttagtagtaa cgagggcacg atagggtgga
     3601 agcccaggta ctgaatattc agcgtcgcga gtacggcata cagcacgccg ccaacgatca
     3661 tcgcacttag cgcgcctttg gcgttggcgc gttcccagta aagacccagc accagcggcc
     3721 acaggaaaac ggcttccagc ccaccgaagg ccagcaaatt cagccagatg atcatttctg
     3781 gcggcttcca ggcggcaagc agcagcaacg cgccgagaac taacgtaatt accgccgaca
     3841 tccgcttcag acgcgtctcg ttttgcattt gatccggacg gatattcaga tagagatctt
     3901 taatgatcgt agcggaactt tgcagcagtt gggcgttaat tgtcgacatg atcgcagcca
     3961 taggtgcagc cagaaagatc ccggcagcaa aaggtggcag cacttttacc attaacgtcg
     4021 ggatcaccag atctggtacg gtgagatcgg ggatcactgc acgacctaac gctccggcca
     4081 ggtgcatacc gaacatcagg attgcgacta taatcgtacc gatgatgatc ccccggtgta
     4141 ctgctttgct gtctttataa gagatacagc gcaccgcagt atgcggcagg ccaatcacgc
     4201 caaagcacac cagtacccag aacgacgtca taaaggcagg cgacagaata tcgtcagcgc
     4261 cttgtggcgt aaccagttgc ggatcgatgg tttgcaaggt ctgtactgcg ttgcttaagc
     4321 cgccagcggc atgtaccacg ccaataagca gcacaacggt gccaatcagc atcacaagcc
     4381 cttgcatggt gtcgttcagc acgctggcgc gaaagccacc aaaggcggta tataacgcaa
     4441 tgctgatacc aaaaatcagc agcccggttt cataaggaat acccgccgcg gtttccagca
     4501 ggcgcgcacc gccgataaac tgcacggtca tcgcaccaac gaacgcaacc agcaaactca
     4561 aactcgccag ccacaccaga agacgactct ggtagcgggc aaacagcata tcgttcagcg
     4621 tcactgcatt gtagcggcgc gcaagaatcg caaacttctt gccgagaata ccgagtgaaa
     4681 gccagacagc aggaagctga atcatcgcca gcaataccca gcccagcccg tatttataag
     4741 cagctcctgg cccgccgata aacgaactgg cactgatata ggtcgcggtg agagtcatcg
     4801 ccagcacaat accgcccata gagcggctgc cgaggaaata ctcattaagg aaggtgccgg
     4861 tgctccgttt acgcatcgca taaaccgaga taccgaacac caccaccaga taggcgacca
     4921 gcggtagaat tacttcaagc tgcatcgtca tcctccagtg ggatatcgcg atagataaat
     4981 ttcaccatcg cccagcacag tccaataaac agtagcggcg tcaggatgca ggccatctca
     5041 aaccagcgcg gaaagccggt aaaaccgggg gcaacgccag gtaagtaagc gtctactaac
     5101 caaactgcca gatacaaaag ggtcagcccc agcgcccagc gggcctcttt atgggcctga
     5161 acaaaacgag tgtccatttt ttgtccctga tgggtgaaga aagcgaggat tgtaccttat
     5221 gggggttgtc gatccccagt aataaaaaag gccggaaaat ccggcctttt gacgcttttg
     5281 cagtcttatt tttcctgaag accgagtttt ttctccagat agtggatgtt agtgccacca
     5341 tgctggaagt tctcgtcatt catgatgcgg atctgcagat caacgttggt tttgataccg
     5401 tcgatgatca gctcctgcag cgcattcttc atgcgggcaa tcgccacgtc acggttttca
     5461 ccgtagcaaa tcagcttacc gatcattgag tcatagtacg gcggtacggt gtagcccgcg
     5521 tagatatgag actcccaacg tacgccaaaa ccgccaggtg cgtggaaacg ggtgattttg
     5581 cctgggcttg gcaggaaggt gttcggatct tcggcgttga tacgacattc caccgcatgg
     5641 ccgcgaacgt gaacttcttc ttgcttgatc gacagcggtt gaccggcagc gatacgcagc
     5701 tgttctttga tcaggtcaac gccggtgatc atttctgtaa ccgggtgttc tacctgaata
     5761 cgggtgttca tttcgatgaa atagaactcg ccgttttcga acaggaactc gaaagtacct
     5821 gcaccgcgat agccgatatc aacacacgct ttagcgcaac gttcgccgat gtagcgacgc
     5881 agttccgggg taatgcccgg tgctggtgct tcttcgacca ctttctggtg gcggcgctgc
     5941 atggagcagt cacgttccgc cagatagata gcgttgccct gaccgtcagc cagtacctga
     6001 atctcgacgt ggcgaggatt ctccaggtat ttctccatgt aaaccatatc gttgctgaaa
     6061 gcagctttcg cttccgcacg ggtcatggag atggattgtg ccagttcagc gtcgccgcgc
     6121 actacgcgca taccgcgacc gccgccgccg ccggaggctt tgataatcac cggataacca
     6181 atgcgtttag caatggcgcg gtttttatcc atatcgtcgc ccagcgggcc gtcagaacca
     6241 ggtacgcaag gaacgcccgc ttttttcatc gccgcgattg cggatacttt gtcgcccatc
     6301 aggcgaatgg tttctgcttt cgggccgatg aagataaagc cggagcgttc aacctgctcg
     6361 gcaaagttgg cgttctcgga gaggaagccg taacccggat ggattgctac tgcgccggtg
     6421 atttcagcgg cgctgatgat tgccgggatg ttcagataac tttttactga cggagcaggg
     6481 ccaatacaga ccgtttcatc tgccagtaat acgtgtttta gatcgcgatc cgtgctggag
     6541 tgcacagcga cagtcttgat gcccagttct ttacaggcac gaagaatacg caatgcaatc
     6601 tcgccgcggt tggcaataac aattttatcc agcatgttcg cctcgttact cgatgacgac
     6661 cagcggctcg tcaaattcta ccggttgtcc actttcgacc agaattgctt tcacggtacc
     6721 ggatttgtcc gcttcgatct ggttcatcat tttcatggct tcaacgatgc acagggtatc
     6781 gcccacgttg actttctgac ccacttcgat gaacgctttt gcgtccgggc ttggggtgcg
     6841 gtagaaagta ccaaccatcg gggaacgtac gatgtgacca ctgatttccg ctgctgctgg
     6901 cgcttccatg gaaggaacgg tcgccggagc ggctgcgtta gattgagctg gctgctgcat
     6961 cattggtgca gcgtaagctt gttgcatcac agggaaactt gcggcaggag ctgcacggct
     7021 aatgcgtact gactcttcgc cttcagaaat ttccagttcg gagatgcctg attcttcaac
     7081 cagctcgatc agttttttaa tcttacgaat atccatgagt gggttccgta ctctttgttt
     7141 agtgtgattg tgacaggcgt ttcaccgccg tctgtaaagc gtatgccccg acagctgtat
     7201 gcatagcgat aaattccagc aggccgggac gccggtctat tttgccccag agtgtcaatg
     7261 ttagacttga cggacattgt gcagcgatct gctgctatcc tccggcaaaa aacaaaatat
     7321 accttcgttg cgccaatgtc accttttccg cagcgcaaaa actgcgtcag ggaggacgag
     7381 gtcgcacatt ataacgattt cgtagcaatt ggcagctaaa tactggtctt atcagggaat
     7441 ataatcaaca gctaacatgt aaataacctt caacaccgtg taatttgcaa caagccgcac
     7501 aattcacgaa attagcgcca tcatcgacgg aacttcttat aacgtaaggt gaaaagctga
     7561 aaagccagcc ctgcgaagat gaggggctgc aatgagataa tcttcacaga ccacaaataa
     7621 tgtagggatg ccaggatcgc gacaagatag acgacattac gcagcggttg ctggggatta
     7681 cccaattttc actgcatcgt ctggatagat gtgaatgcta aagcacgcaa aatcaaccaa
     7741 ctgacaattc tcagcattaa ataggttcga gtggttaatt tgtagtcaaa taaaatcaga
     7801 ttattcaccc catggtacca gaaatcagta acaggttaag cgtagtgtcg cccaggcaaa
     7861 gcaccataaa cctaacaggc gacggaggcg tatcaataat ggctgtttag aggagtatca
     7921 atgcgcatca atttgtggtc agtcgaaatc atgactttcc agtttggatg atgttgtaca
     7981 acttcctgac caaggtgttc gacgaaagct gtccggctaa ccgcttttcc cgttaaccga
     8041 ttgaataatg atgtcaaaat gagttccctc tcttattatt ccttgctaaa tggttagtta
     8101 accttcacca gcgtgcgacc ctggatctgg ttattaatga tggcctcggc aaagttcggt
     8161 gcctctgaca gagatatctc ttttgccgcc tgggtataga atgattccgg taaatcggcg
     8221 accagtcgct gccaggcttg tgcgcggcgt tctggtggcg tcattactga atccacccct
     8281 tgcaaacgga cattacgcag aataaatggc atgaccgtgg ttggcagagt aaaaccaccc
     8341 gccagaccac aggccgccac gcagccgccg taattcattt gcgccagcac ttttgccagc
     8401 actttgtcgc caacagtgtc aattgcccca gcccagactt gtttttccag aggacgggat
     8461 tcggcaaact catcacgagg gagaataccg ctggcaccta aacttttcag atattcatgg
     8521 gtactttcgc gaccggaaac ggcaacgacc tgataaccca acttatgcag cagcgccacg
     8581 gcggtactgc cgacgccacc actggcaccc gtcacgacaa tctcaccgtc ctgcgggcga
     8641 acaccggcat cttccagcgc catcacacac agcatggcgg taaaaccggc agtaccgata
     8701 atcattgctt tacgcgcgtc cagcccttgc ggcatggcaa ccagccagtc acctttcact
     8761 cgcgcctgct ccgccagccc accccagtgg ttttcaccaa cgccccagcc agtgagtaac
     8821 acctcctgac cggcatgaaa acgcggatct tcgctggtgc gtacagttcc ggcaaaatcg
     8881 atcccaggaa tcatcggaaa attacggatg atttttccct taccggtaat cgccagcgca
     8941 tctttatagt tcaggctcga ccagtgaaca tcgaccgtga catcgccctc cggcaggcga
     9001 cgttcgtcca gagtctgtac tgatgcgaga gttttgccgt cctgctgttc taaaagtaac
     9061 gcctgcataa gtggtcctca tgtgcatgat ggattggaaa ataatttctg aagactatac
     9121 tcgctaatgg aaaagcaaag ccgatgaagc gcaataaatt gcgagataaa tctgatttgc
     9181 tagtatgccc gcttcctcac tatcggagtt aacacaagga tgagattaac gacgaaattt
     9241 tcggcctttg ttacgctgct caccgggtta acaatttttg tgactttgct gggctgttcg
     9301 ctaagtttct acaacgccat tcagtataag tttagtcatc gcgttcaggc ggtggcgacg
     9361 gcgattgata cccaccttgt gtcgaatgac ttcagcgcat taaggccaca aattaccgaa
     9421 ttaatggtgt cagcagatat cgttcgtgta gacctgctgc atggtgataa acaggtttat
     9481 accctggcca gaaatggtag ttatcgtccg gttggcacca gcgatctgtt tcgtgaactg
     9541 agcgttccgt tgataaagca tccggggatg tcgttgcgtc tggtttatca ggatccgatg
     9601 ggcaactatt tccattcgtt gatgaccacc gcgccgctca cgggggcgat tggctttatc
     9661 attcttatgc tctttctggc ggtacgctgg ttacaacggc aacttgccgg gcaagaattg
     9721 ctggaaaccc gggctactcg tatcttaaac ggtgagcgtg gctctaatgt gttgggaacc
     9781 atctatgaat ggccgcccag aaccagcagt gcgctggata cgctgcttcg tgaaattcag
     9841 aacgcacgcg aacaacacag ccgtcttgat acgctgatcc gctcttatgc cgcacaggac
     9901 gtgaaaaccg gcctcaataa ccgactcttt ttcgataatc agttagcaac gttactggaa
     9961 gatcaggaga aagtaggtac ccacgggatc gtgatgatga ttcgtctgcc ggatttcaat
    10021 atgttgagcg atacctgggg gcacagccag gttgaagaac agttcttcac tctgacgaat
    10081 ctgctgtcga catttatgat gcgctaccct ggcgcactgc tggcgcgtta ccaccgcagt
    10141 gattttgctg cgctgttacc gcaccggacg ttaaaagagg cagagagcat agccagtcag
    10201 ttaatcaaag ccgtcgatac cttgccgaac aataaaatgc tcgatcgcga cgatatgatc
    10261 cacattggta tctgtgcctg gcgtagtggt caggataccg agcaggtaat ggaacatgca
    10321 gagtctgcca cgcgtaatgc gggattgcag ggtggcaata gctgggctat ttatgatgac
    10381 tcgttgcctg aaaaaggacg cggtaatgtt cgctggcgta cgcttatcga gcaaatgctc
    10441 agtcgcggcg gcccgcgcct ttatcaaaaa ccggcggtta ctcgcgaagg tcaggttcat
    10501 catcgcgaac tcatgtgccg catcttcgat ggtaatgaag aggttagctc ggcggagtat
    10561 atgccgatgg tcttgcagtt tggcttatcg gaagagtatg accgtctgca aatcagccgt
    10621 cttattccac tattgcgtta ctggccagag gaaaatctgg cgattcaggt taccgttgag
    10681 tcgctgattc gtccgcgttt tcagcgttgg ctgcgcgata cgttaatgca atgtgaaaaa
    10741 tcacaacgaa aacgcataat tattgaactt gcagaggccg atgtaggtca acatatcagt
    10801 cgtttacaac ctgttattcg tttagtgaat gctttagggg tacgggtagc cgtcaaccag
    10861 gctggtttga cgctggtaag caccagttgg atcaaagaac ttaatgttga gttactcaag
    10921 ctccatccgg ggctggtcag gaacattgag aagcgaacgg agaaccagct gctggttcaa
    10981 agcctggtgg aagcctgctc cgggaccagc acccacgttt acgccaccgg cgtgcgttcg
    11041 cgaagcgagt ggcagaccct gattcagcgc ggtgttacag gcgggcaagg ggattttttc
    11101 gcgtcctcac agccacttga tactaacgtg aaaaaatatt cacaaagata ctcggtttaa
    11161 cctgccgttt aatccgtttt cacgtagaat aacgcgcgct gcgtctcatg ggagtgtgct
    11221 tgtctgctcg ccagattgtt gcagcacata tgcagatgaa tgaccttacg cggttgcaaa
    11281 caggcgagga atgctgctga tgcattaagc ctttctggac tcaggcagag atttgtaaca
    11341 aaggaaacga actgcactaa ttttcaccgt agcagatgat ttttgcgcct tgtcgctgct
    11401 gcgtgtggtt ggtaaagtaa gcggattttc ttttccgccc cagctttcag gattatccct
    11461 tagtatgttg aaaaaatttc gtggcatgtt ttccaatgac ttgtccattg acctgggtac
    11521 tgcgaatacc ctcatttatg taaaaggaca aggcatcgta ttgaatgagc cttccgtggt
    11581 ggccattcgt caggatcgtg ccggttcacc gaaaagcgta gctgcagtag gtcatgacgc
    11641 gaagcagatg ctgggccgta cgccgggcaa tattgctgcc attcgcccaa tgaaagacgg
    11701 cgttatcgcc gacttcttcg tgactgaaaa aatgctccag cacttcatca aacaagtgca
    11761 cagcaacagc tttatgcgtc caagcccgcg cgttctggtt tgtgtaccgg ttggcgcgac
    11821 ccaggttgaa cgccgcgcaa ttcgtgaatc cgcgcagggc gctggtgccc gtgaagtctt
    11881 cctgattgaa gaaccgatgg ctgccgcaat tggtgctggc ctgccggttt ctgaagcgac
    11941 cggttctatg gtggttgata tcggtggtgg taccactgaa gttgctgtta tctccttgaa
    12001 cggcgtggtt tactcctctt ccgtgcgcat tggtggtgat cgtttcgacg aagctatcat
    12061 caactatgtg cgtcgtaatt acggttctct gatcggtgaa gccaccgcag aacgtatcaa
    12121 gcacgaaatc ggttcggctt acccgggcga tgaagtccgt gaaatcgaag ttcgtggccg
    12181 taacctggca gaaggtgttc cacgcggttt taccctgaac tccaacgaaa tcctcgaagc
    12241 actgcaggaa ccgctgaccg gtattgtgag cgcggtaatg gttgcactgg aacagtgccc
    12301 gccggaactg gcttccgaca tctccgagcg cggcatggtg ctcaccggtg gtggcgcact
    12361 gctgcgtaac cttgaccgtt tgttaatgga agaaaccggc attccagtcg ttgttgctga
    12421 agacccgctg acctgtgtgg cgcgcggtgg cggcaaagcg ctggaaatga tcgacatgca
    12481 cggcggcgac ctgttcagcg aagagtaatc ggatgcaggc aggggaagcg tctgtttacc
    12541 ctgcctggtc tgatacgaga atacgcataa cttatgaagc caatttttag ccgtggcccg
    12601 tcgctacaga ttcgccttat tctggcggtg ctggtggcgc tcggcattat tattgccgac
    12661 agccgcctgg ggacgttcag tcaaatccgt acttatatgg ataccgccgt cagtcctttc
    12721 tactttgttt ccaatgctcc tcgtgaattg ctggatggca tatcgcagac gctggcctcg
    12781 cgtgaccaac tagaacttga aaaccgggcg ttacgtcagg aactgttgct gaaaaacagt
    12841 gaactgctga tgcttggaca atacaaacag gagaacgcgc gtctgcgcga gctgctgggt
    12901 tccccgctgc gtcaggatga gcagaaaatg gtgactcagg ttatctccac ggttaacgat
    12961 ccttatagcg atcaagttgt tatcgataaa ggtagcgtta atggcgttta tgaaggccag
    13021 ccggtcatca gcgacaaagg tgttgttggt caggtggtgg ccgtcgctaa actgaccagt
    13081 cgcgtgctgc tgatttgtga tgcgacccac gcgctgccaa tccaggtgct gcgcaacgat
    13141 atccgcgtaa ttgcagccgg taacggttgt acggatgatt tgcagcttga gcatctgcca
    13201 gcgaatacgg atattcgtgt tggtgatgtg ctggtgacct ccggtctggg cggtcgtttc
    13261 ccggaaggct atccggtcgc ggttgtctct tccgtaaaac tcgataccca gcgcgcttat
    13321 actgtgatcc aggcgcgtcc gactgcagga ctgcaacgtt tgcgttatct gctgctgctg
    13381 tggggcgcgg atcgtaacgg cgctaacccg atgacgccgg aagaggtgca tcgtgttgct
    13441 aatgaacgtc tgatgcagat gatgccgcag gtattgcctt cgccagacgc gatggggcca
    13501 aagttacctg aaccggcaac ggggatcgct cagccgactc cgcagcaacc ggcgacagga
    13561 aatgcagcta ctgcgcctgt tgcgccgaca cagcccgctg ctaatcgctc tccacaaagg
    13621 gctacgccgc cgcaaagtgg tgctcaaccg cctgcgcgtg cgccgggagg gcaatagtgg
    13681 cgagctatcg tagccaggga cgctgggtaa tctggctctc tttcctcatt gcgctgttgc
    13741 tgcaaatcat gccctggccg gataacctga ttgttttccg gccaaactgg gtgttactta
    13801 tcttgttgta ttggatcctg gccttgcccc atcgcgtaaa tgtgggcaca ggttttgtga
    13861 tgggtgccat actggatctg atcagcggct cgatgcttgg cgtacgcgta ttggcgatga
    13921 gcatcattgc ttacctggtg gcgctgaaat accagctttt ccgcaacctc gcattatggc
    13981 agcaggcgct ggtcgtcatg ttgctttcgc tggtggtgga tattattgtt ttctgggcag
    14041 agtttttagt gattaacgtc tctttcagac cggaagtgtt ctggagtagt gtagtcaatg
    14101 gggtgctctg gccgtggatt ttcttgctga tgcgtaaagt ccgtcagcag tttgcagtgc
    14161 aataaaggtt tctatgactt ctctgtattt agcttccggt tctccgcgtc gtcaggagtt
    14221 acttgcgcaa cttggcgtga cctttgaacg tattgttacg ggcattgagg agcagcgtca
    14281 gccgcaggag agcgcgcagc agtatgttgt gcgtctggca cgcgagaaag cacaggcggg
    14341 tgtcgcgcaa acggcacagg atctcccggt gctgggtgcg gatattatcg ttatcctgaa
    14401 cggagaagtg ctcgagaaac cgcgcgacgc agagcatgcg gcgcagatgt tgcgcaaatt
    14461 atcgggtcag acccatcagg tgatgacggc agtggcgttg gccgacagcc agcacattct
    14521 cgattgcctg gtggtcaccg atgtgacttt cagaacgtta acagacgaag acatcgcggg
    14581 ctatgtcgcc agcggtgaac cgttagataa agcaggtgca tacggtattc aggggctggg
    14641 tggctgtttt gtcaggaaga taaatggcag ctatcacgcc gtagtcggct taccgctggt
    14701 tgaaacgtat gaattattaa gtaattttaa cgcactgcgt gagaaaaggg ataaacatga
    14761 cggctgaatt gttagtaaac gtaacgcctt cggaaacgcg agtggcgtat attgatggcg
    14821 gtattctgca ggaaattcat attgaacgtg aggcgcgacg cggaatagta ggcaatatct
    14881 acaagggtcg tgtaagtcgt gtacttccgg gtatgcaggc ggcttttgta gatattgggc
    14941 tggataaagc cgcgtttctt catgcatccg acatcatgcc gcataccgaa tgtgtggcgg
    15001 gtgaagaaca aaagcaattc acggtgcgcg acatctcgga actggttcgt caggggcaag
    15061 atctgatggt gcaggtggtg aaagatccgc ttggcactaa aggtgcgcgc ctgaccaccg
    15121 atatcacgct gccttctcgc tatctggtat ttatgccagg ggcttctcac gttggggttt
    15181 cccaacgaat tgaaagcgaa tcagaacgtg aacgtctgaa aaaagtggtc gcagagtatt
    15241 gcgacgagca gggcgggttt atcatccgca ccgcagcgga aggggttggc gaggctgaac
    15301 tggcctccga tgccgcttat ctgaaacgcg tctggaccaa agttatggag cgtaaaaagc
    15361 gcccgcagac ccgttatcag ctgtacggcg aactggcgct ggcgcagcgt gttctgcgtg
    15421 attttgccga cgccgaactg gaccgcattc gcgttgactc acgcctgact tacgaagcat
    15481 tgctggagtt tacctcggag tacattcccg agatgaccag caagctggag cattacaccg
    15541 gacgccagcc gattttcgat ctctttgatg tcgaaaacga aatccagcga gcgttggaac
    15601 gcaaagtaga actgaaatcc ggtggttatc tgattatcga ccagaccgaa gcgatgacca
    15661 ccgtggacat caataccgga gcgtttgtcg gtcatcgcaa tctggacgac actattttca
    15721 ataccaatat tgaagcgacg caggctatcg ctcgccagtt acggttgcgt aatctgggcg
    15781 ggattatcat tattgatttc atcgatatga ataatgaaga tcaccgccgc cgcgtgctgc
    15841 attcgctgga gcaggcgttg agcaaagacc gggtgaaaac cagcgttaat ggtttttcgg
    15901 cgctggggct ggtggagatg acgcgtaaac gcacccgcga aagcattgag cacgtactgt
    15961 gtaacgaatg cccaacctgc cacggtcgcg gaacggtgaa aaccgtggaa acggtatgct
    16021 atgaaatcat gcgcgagatt gttcgtgtcc accatgctta cgactccgac cgtttcctgg
    16081 tctatgcttc tccggcagta gccgaagcct tgaaaggcga agagtcacac tcgctggcgg
    16141 aagtggaaat tttcgttggc aaacaggtta aagtacaaat tgaaccgctc tataaccagg
    16201 agcagtttga cgtcgtaatg atgtaaacag atgctgggcc gccatccggc aaagggtttt
    16261 tgagtcacat ttttagcaga caaggagtga cgggtgaggc gattgccggg gattttactg
    16321 cttactggag ccgcgctcgt tgtgatcgct gccctgctgg ttagcggcct gcgtattgct
    16381 ttaccgcatc ttaacgcctg gcgtccggaa atcctcaaca aaatagaatc cgcgactggc
    16441 atgccggtag aagccagtca gctctcagcc agctggcaga attttggccc gacgcttgaa
    16501 gcacacgaca tccgtgcaga actaaaagat ggcggcgaat tttcggttaa acgcgttact
    16561 ctggcgctgg atgtctggca gagcctgtta catatgcgct ggcagtttcg cgacctcact
    16621 ttctggcagc tgcgctttcg caccaacact cctatcacca gcggtggtag cgatgacagt
    16681 ctggaagcca gtcacatcag cgatctgttt cttcgtcaat ttgaccattt cgatctccgc
    16741 gacagtgaag tcagttttct gacgccatcc ggtcagcgcg ccgagctggc gatcccacaa
    16801 ctcacctggc tgaacgatcc acgtcgacac cgtgcggaag gcctggtaag cctctccagc
    16861 cttaccggac agcacggcgt gatgcaggtg cgcatggatt tgcgcgatga tgaggggttg
    16921 ttaagcaatg gtcgcgtctg gctacaggcg gatgacatcg acctgaagcc gtggctcggt
    16981 aaatggatgc aggacaatat tgctctggaa acggcacagt tctcccttga aggctggatg
    17041 acgatcgaca aaggcgatgt aaccggcggt gacgtctggc tgaaacaggg cggtgccagc
    17101 tggttgggcg agaagcaaac gcatacgctg tcggtggata atctgaccgc gcatattacg
    17161 cgtgaaaatc cgggctggca gttctctatt cccgatacac ggatcacgat ggacggcaaa
    17221 ccctggccga gcggagcatt gacgctggcc tggataccgg aacaggacgt tggcggcaaa
    17281 gacaataaac gcagtgacga actccggatt cgcgctagta atctggagct ggcaggcctg
    17341 gagggcatac gcccgctggc cgcgaaactt tcacctgcac tgggtgatgt ttggcgctcc
    17401 acacaaccga gcggcaagat taacactctg gcgctggata tcccgcttca ggcggcagac
    17461 aagacccgtt ttcaggcatc gtggagcgat ctggcctgga agcaatggaa attattaccg
    17521 ggtgcggaac acttctccgg gacgctttcc ggcagcgttg aaaatggttt gcttaccgcg
    17581 tcgatgaagc aggcaaagat gccttacgaa acggtattcc gtgcgccact ggaaatcgcc
    17641 gacggccagg caactataag ctggctgaac aatgacaaag gtttccagct ggatgggcgt
    17701 aatattgacg ttaaagccaa agccgtccat gcgcgcggcg gttttcgtta cctgcaacct
    17761 gctaacgatg aaccctggct gggtattctg gctggcatca gtaccgatga tggttcacaa
    17821 gcctggcgct atttcccgga aaacttgatg ggtaaagacc tggttgatta tttaagtggt
    17881 gcgattcagg gcggtgaagc ggataacgcg acgctggttt atggtggcaa tccgcaactc
    17941 ttcccctata aacacaacga aggtcagttt gaagtgctgg tgccgctgcg caacgcgaag
    18001 tttgccttcc agccggactg gcctgcatta actaaccttg atattgaact ggactttatt
    18061 aacgacggtt tatggatgaa aaccgatggc gttaatctgg gcggcgtgcg cgcgagtaat
    18121 ctcaccgcag tgatccctga ctactcaaaa gaaaaactgc tgattgacgc tgacattaaa
    18181 ggtccgggta aagccgttgg cccttacttt gatgagacac cgctgaaaga ttctctgggt
    18241 gcgaccctgc aagaactcca gctcgacggc gatgtgaatg ctcgcttaca tcttgatatc
    18301 ccgctgaacg gcgaactggt aaccgcgaaa ggtgaagtga cgctgcgtaa taacagtctg
    18361 tttatcaaac cactcgacag catcctgaaa aatttgagcg gtaaattcag ctttatcaat
    18421 ggcgatctgc aaagtgaacc actgacagca agctggttta atcagccgtt gaacgtggat
    18481 ttttccacca aagaaggggc aaaagcctac caggttgcgg tgaatctcaa cggtaactgg
    18541 caaccggcga aaaccggcgt tctgcctgaa gcggtgaacg aagcattgag tggcagcgtg
    18601 gcgtgggatg gtaaagtggg cattgatctg ccttatcatg ctggcgcgac ctataacgta
    18661 gagctgaacg gcgatctgaa taatgtgagt agtcacttac cttcaccgtt agccaaacct
    18721 gcgggtgaac cactagcggt aaacgttaag gttgatggca atctcaacag ctttgaatta
    18781 accggacagg ctggtgcgga taaccatttc aatagccgct ggttgcttgg tcaaaagctg
    18841 acgctcgatc gtgctatttg ggcggcagac agtaaaacgc tcccgccgtt gccggaacaa
    18901 agtggcgttg aacttaatat gccgccgatg aatggtgccg agtggctggc cctgttccag
    18961 aaaggcgctg cggagagtgt cggtggtgca gcgagtttcc cacaacacat aacgttacgt
    19021 acgcctatgt tgtcactggg aaatcagcaa tggaataacc tgagtattgt ttcgcaaccg
    19081 acggcaaatg gcaccctggt tgaggcgcaa gggcgtgaaa tcaacgccac gctagcgatg
    19141 cgtaataacg cgccgtggct ggcgaatatc aaatatcttt attacaaccc gagcgtggcg
    19201 aaaactcgtg gtgattcaac gccgtcatca cctttcccga caacggagcg cattaacttc
    19261 cgtggctggc cggacgcaca aatacgatgc acagagtgct ggttctgggg gcaaaaattc
    19321 ggtcgcattg acagtgatat caccatttct ggcgatacgt taacgctgac caatggactg
    19381 attgatactg gtttctcgcg gcttactgcc gatggtgaat gggttaataa tccggggaat
    19441 gaacgtacct cgctgaaagg aaaactgcgt gggcagaaaa ttgatgccgc cgcagaattt
    19501 tttggtgtca cgacgcccat acgccagtcg tcatttaatg tggattacga tttacactgg
    19561 cgcaaagcac cctggcagcc agatgaagcg acgttgaatg gcatcattca tactcaactg
    19621 ggtaaaggcg aaattaccga aatcaatacc ggacatgccg ggcaattgct gcgcttattg
    19681 agcgtagatg ccctgatgcg taagctgcgt tttgatttca gagacacttt tggcgaaggg
    19741 ttctattttg actccattcg cagcaccgcg tggattaaag acggcgttat gcacaccgac
    19801 gacacgctgg tggatggcct ggaggcggat atcgccatga aagggtcggt aaatctggta
    19861 cgccgcgacc tgaatatgga agcggttgtc gcaccagaga tttctgcgac ggtgggcgtg
    19921 gctgcggctt ttgcggttaa ccccattgtt ggcgcggcag tgtttgccgc cagtaaagtg
    19981 ctggggccgc tgtggagcaa agtctccatt ttgcgctatc acatttcggg tccgctggac
    20041 gatccgcaaa tcaacgaagt gttgcgccaa ccgcgtaaag aaaaagcgca atgatttgac
    20101 gagggcgcgt aattgcccca atctcatatg ataatcgttg ccaaaggcca acgagccgga
    20161 acataactgt aggccggata gggcgttcac gccgcatccg gcagccgtaa aaaatcctct
    20221 actgcagtaa ctaacgagta gcaaaaacga tgagtctgaa cctggtaagt gaacaattgc
    20281 tagcggcgaa cggcctgaaa catcaggact tgttcgcgat cctcggtcaa ctggccgaac
    20341 gtcgccttga ttatggcgat ctctattttc agtcgagcta tcacgaatcc tgggttttag
    20401 aagaccgcat tattaaagat ggttcttaca acatcgatca gggcgttggc gtgcgtgcaa
    20461 tcagcggtga aaaaaccgga tttgcttacg ctgaccaaat cagcctgctg gcgctggaac
    20521 agagtgcgca agcggcgcgc accatcgtcc gtgatagtgg tgatggcaaa gtacagacgc
    20581 tgggcgcggt agagcatagc ccgttgtata cctcggtaga tccgctgcaa agcatgagcc
    20641 gtgaagagaa gctggatatc ctgcgtcgcg tcgataaggt tgctcgcgaa gcggacaagc
    20701 gcgtacagga agtgactgcc agcctcagtg gtgtctatga attaattctg gttgcggcca
    20761 ccgacggcac gctagcggcg gatgtccgtc cgctggtgcg tctttccgtg agcgttctgg
    20821 tcgaagaaga tggcaaacgc gaacgcggtg ccagtggcgg cggcggtcgt tttggttatg
    20881 agttcttcct tgccgatctt gacggcgaag tccgtgcgga tgcatgggca aaagaagcag
    20941 tgcgtatggc gctggtcaat ctttctgccg ttgctgcacc agcgggcacc atgccggtag
    21001 tacttggcgc aggttggccg ggcgtgctgt tgcatgaagc ggtaggtcac ggtctggaag
    21061 gcgacttcaa ccgccgtggc acttcagtat ttagtggaca ggtcggggag ctggtggctt
    21121 cagaactgtg taccgtggtt gatgacggca cgatggtcga tcgccgtggt tcggtggcga
    21181 ttgatgatga aggtacgcca ggccagtaca acgtgctgat tgagaacggc attctgaaag
    21241 gctacatgca ggataaactc aacgcgcgtt tgatggggat gacgccgact ggcaacggtc
    21301 gccgtgaatc ctacgcccat ctgcccatgc cgcgtatgac caacacctat atgctgccgg
    21361 gtaaatcgac cccgcaagaa attattgaat ccgttgagta cggtatctat gcaccgaact
    21421 ttggtggcgg tcaggtggat atcacctccg gcaaattcgt tttctccact tcagaagcat
    21481 atctgattga aaacggtaaa gtaacgaagc cggtgaaagg cgcaacgttg attggttccg
    21541 gtatcgaaac catgcagcag atttcgatgg ttggcaacga cctgaaactg gataacggcg
    21601 tgggtgtctg cggtaaagaa gggcaaagtt tgccggttgg cgtgggccag ccaacgttga
    21661 aagtcgataa cctgactgtt ggcggtactg cgtaataatt tcattatttt cagaaggata
    21721 attaatcttt ctacgtgcac gaacggtccc ctcgctcctc tggggttagg gtgaggggaa
    21781 cccgttggca caggtttgta gaacgtaaca gtacaatatg aattacttct ctttcccgcg
    21841 cccgtgcatc tcctgaaaca atttaccgac ctcaacaaaa taatccgtca gcgagttgat
    21901 cacgacctgt accttcagcg gcagcttatc tttttcggta tataacgcat aaaccgggcg
    21961 tggatctgac tgataacgcg gcagcaggat ctccagctcc ccacgattga tctcgttgat
    22021 cacccacatc agcggcacgt aggcgatccc ggcacccgcc gttagccagc gcaccagcgt
    22081 catcggatca ttagtcacaa atctcccttg cgggatcagg cgagtcgaga tcccttccgg
    22141 tgcaatcagt tcaaattcat tgtcgggccg cacgctgtat tcaagccatg aatggctact
    22201 taaatcggcg ggtttttccg gtatgccgta ttgtgtgaga tagcttttcg cggcgcacac
    22261 caccattggc atcgcgccaa gacggcggga aaacaggctg gaatcctgca acgcgccgac
    22321 gcggatcacc acatccagac cgtcggcaat caggtcgggg gctggaattc cggtaaccag
    22381 attggcgctc aaacctgggt attctttcag cattttggct gtcagcccgg cgagaacatt
    22441 ttgtgccata gttgaagaac agccaatgcg tagcgtcccg atgggggtgt tattgaaggc
    22501 atacagttgc tcatgaacat cctgcacttc atgaagcata cgacggcagc cctggtagta
    22561 aattctaccg gcttcggtca ggccaatgct gcgtgtgcta cggtttaaca gctttacctg
    22621 caactcatct tccagttttg acaccgtctg actgatggac gaaacgctca tctgtagctg
    22681 tctggcggcg gcggtaaaag agccaaattc aactactttg gcaaacaccg acatgcgttt
    22741 tagtcgttcc attattcact ctgacttaaa agtgatttag atcacataat atagataaca
    22801 gcataacagt tacgctaata tattaaatat caatctacag caatgttgct ctcgcccggc
    22861 tttccatgcc cttctctgcg gcgacagatg ctgaaaataa taacgcctgc tctctctata
    22921 caaccaaggt caacatgagt ctgtttcccg ttatcgtggt gtttgggctg tccttcccac
    22981 cgatattttt tgaattgctt ttatcactgg cgattttctg gctggtgcgc cgggtacttg
    23041 tgccaacagg tatctacgac tttgtctggc atccggcgtt gttcaacacc gcgctctatt
    23101 gctgcttgtt ttatttgata tcgcgactgt tcgtttgagg ttgaagtgaa aacactaata
    23161 agaaaattct cccgtacggc catcacggtc gtattagtca ttctggcctt catcgcaatt
    23221 tttaatgcct gggtctatta caccgaatcc ccctggacgc gtgacgcgcg ctttagcgct
    23281 gacgtcgttg cgatcgcgcc ggacgtttct ggactcatta cccaggtgaa tgttcatgat
    23341 aaccagctgg tgaaaaaagg acagatactg ttcaccatcg accagccgcg ctatcaaaag
    23401 gcgcttgagg aagcgcaagc cgatgttgct tattatcagg tactggcaca ggagaaacgc
    23461 caggaggccg gacgtcgtaa ccgtctcggt gtgcaggcga tgtctcgcga agagatcgac
    23521 caggctaaca acgtactaca aacggttctg catcagttag cgaaagcgca ggcgacccgc
    23581 gatctggcaa aactggatct tgaacgcacg gtgatccgag cgccagcaga tggctgggtg
    23641 accaacctca acgtctatac cggtgagttt attactcgag gatcaacggc ggttgcgctg
    23701 gtgaaacaga actccttcta tgtactggcc tatatggaag aaactaagct ggaaggggtg
    23761 cgtccggggt atcgtgcaga gatcacgccg cttggcagta acaaagtgct gaaagggact
    23821 gttgatagtg ttgccgctgg ggtcaccaac gccagcagca cgcgtgacga caaagggatg
    23881 gcgaccatcg actctgacct tgaatgggtg cgtttggcgc aacgtgttcc ggttcgtatt
    23941 cgtctcgaca accagcaaga gaacatctgg cctgcgggca ccactgctac agtggtggtc
    24001 actggcaaac aagatcgcga cgaaagccag gattcgttct tccgtaaaat ggcccatcgc
    24061 ctgcgtgagt ttggttaatc acgatgggta ttttctccat tgctaaccaa catattcgct
    24121 ttgcggtaaa actggcgacc gccattgtac tggcgctgtt tgttggcttt cacttccagc
    24181 tggaaacgcc acgctgggcg gtactgacag cggcgattgt tgccgccggt ccggcctttg
    24241 ctgcgggagg tgaaccgtat tctggcgcta ttcgctatcg tggctttttg cgcatcatcg
    24301 gcacatttat tggctgtatt gccggactgg tgatcatcat tgcgatgatc cgcgcaccat
    24361 tattgatgat tctggtgtgc tgtatctggg ccggtttttg tacctggata tcctcgctgg
    24421 tacgaataga aaactcgtac gcgtgggggc tggccggtta taccgcgctg atcattgtga
    24481 tcaccattca gccggaacca ttgcttacgc cgcagtttgc cgtcgaacgt tgtagcgaga
    24541 tcgttatcgg tattgtgtgt gcgattatgg cggatttgct cttttctccg cgatcgatca
    24601 aacaagaagt agatcgagag ctggaaagtt tgctggtcgc gcaatatcaa ttaatgcaac
    24661 tctgtatcaa gcatggcgat ggtgaagttg tcgataaagc ctggggcgac ctggtgcgac
    24721 gcaccacggc actacaaggc atgcgcagca acctgaatat ggaatcttcc cgctgggcgc
    24781 gggccaatcg acgtttaaaa gcgatcaata cgctatcgct gacgctgatt acccaatcct
    24841 gcgaaactta tcttattcag aatacgcgcc cggaattgat cactgatact ttccgcgaat
    24901 tttttgacac gccggtagaa accgcgcagg acgtccacaa gcagctcaaa cgcctgcgga
    24961 gagttatcgc ctggactggg gaacgggaaa cgcctgtcac catttatagc tgggtcgcgg
    25021 cggcaacgcg ttatcagctt ctcaagcgcg gcgttatcag taacacaaaa atcaacgcca
    25081 ccgaagaaga gatcctgcaa ggcgaaccgg aagtaaaagt agagtcagcc gaacgtcatc
    25141 atgcaatggt taacttctgg cgaaccacac tttcctgcat tctgggcacg cttttctggc
    25201 tgtggacggg ctggacttcc ggcagtggtg caatggtgat gattgcggtc gtgacgtcac
    25261 tggcaatgcg tttgccgaat ccacgcatgg tggcgatcga ctttatctat gggacgctgg
    25321 ccgcgctgcc gttagggctg ctctactttt tggtgattat ccctaatacc caacagagca
    25381 tgttgctgct gtgtattagc ctggcagtgc tgggattctt cctcggtata gaagtacaga
    25441 aacggcgact gggctcgatg ggggcactgg ccagcaccat aaatattatc gtgctggata
    25501 acccgatgac tttccatttc agccagtttc tcgacagcgc attaggacaa atcgtcggct
    25561 gtgtgctcgc gttcaccgtt attttgctgg tgcgggataa atcgcgcgac aggactggac
    25621 gtgtactgct taatcagttt gtttctgccg ctgtttccgc gatgactacc aatgtggcac
    25681 gtcgtaaaga gaaccacctc ccggcacttt atcagcagct gtttttgctg atgaataagt
    25741 tcccagggga tttgccgaaa tttcgcctgg cgctgacgat gattatcgcg caccagcgcc
    25801 tgcgtgatgc gccgatcccg gttaacgagg atttatcggc gtttcaccga caaatgcgcc
    25861 gcacagcaga ccatgtgata tctgcccgta gcgatgataa acgtcgtcgg tactttggcc
    25921 agttgctgga agaactggaa atctaccagg aaaagctacg catctggcaa gcgccaccgc
    25981 aggtgacgga accggttcat cggctggcgg ggatgctcca taagtatcaa catgcgttga
    26041 ccgatagtta agtcaaaacc gacgccaaaa gcgtcggttt tttcatggct atacttagcg
    26101 atgcacggca gaactcgccg cgaaacgtga cggtggcaac agatgaatat ttatgccttt
    26161 gattttgatg agattgagag tcaggaggat ttttatcgtg actttagcca aacctttggt
    26221 ctggctaaag ataaggtacg cgatctcgac tcactatggg atgtgttaat gaacgatgtc
    26281 ctgccgctac cacttgagat tgaatttgtt catctgggag agaaaacgcg tcgccgtttt
    26341 ggcgcgttaa tattgctgtt tgatgaggca gaggaagagc tggaagggca tttgcgtttt
    26401 aatgttcgtc attagcgcaa aaaaaagccc acgaaccggg ggcaatatcg tcggacaaaa
    26461 cgatgagggt ttatttgtac agttcagccg tagcgtgcca ggtgtcaccg ctacgagctt
    26521 cagtaatctg gtaggccgtt gcgcctttct cttccgcttt tttgttcagc atttcacgta
    26581 tatccattgg cgaagacgcc acaccactta cggatacggt cccgattgct tcacgatttt
    26641 gtgcttgtgc agcatcaatg gagtcggcag cgaatgcacc gaaagagaga acagaaagta
    26701 cgcttaatgc agctacagtg gttttgattt tcatgatttt tacctcgaca taatctttta
    26761 gctgggcctt tgtttcgtga cccatatcac aaaatcaagt atacactaat tattgaacta
    26821 attaatacca cactaactgt ttcagttgat actaagtgtt aaaaatgtga attttaataa
    26881 caaaaaagta taaaaaacac ggttatttat tattaattat agtattttca attagataat
    26941 gaattttgca taatgtgctt tttttaccgt atgtattcat tttgtgaatg atgtgtcgcg
    27001 gtaaaacgca ctattcgtta aatgtatcgc aggagaaaga aggggaatga gagggataag
    27061 caacattttc cccgccgcca gaagcgacgg ggcagagatt aaagctcctg gtcgaacagc
    27121 tctaaaatcg cttcgtacag gtctttgacg gtgaaaccgt tagcaggggt ggtaaagatg
    27181 gtgtcatcgc cagcgatggt gcccagaata ccttctgctt tgcccagtga gtccagcagg
    27241 cgagcaatta actgcgccgc gccagggctg gtatgaatca cgacaactgc atcgttgtag
    27301 tcgatatcca gcaccagatt cttcaatgga ctggaggtgg ttggtacacc cagttcagct
    27361 ggcaggcagt aaaccatttc cattttggca ttgcgtgtac gtacagcacc aaacttggtc
    27421 aacatccgcg agactttaga ctgattaata ttgtcaaagc cttgctcctg caacgcggcg
    27481 acgatttcgc cctgggagct aaatttctct tctttaagta atgctttaaa tgctttaact
    27541 agttcttctt gcttagccga gcttcgcata agtcacccga tatggtggtt gatacaacat
    27601 tattgtgcat acagatgaat ttttatgcaa acagtcagcc ctgaagaagg ctgaaataat
    27661 gttatgaaag agcgggattt tatcaaattt cgttattgag aaacatgcct gcgtcacggc
    27721 atgcaaattc tgtttaaaag taaattaatt gttatcaaat tgatgttgtt ttggctgaac
    27781 ggtagggtat attgtcacca cctgttggaa tgttgcgcta atgcataagc gactgttaat
    27841 tacgtaagtt aggttcctga ttacggcaat taaatgcata aacgctaaac ttgcgtgact
    27901 acacattctt gagatgtggt cattgtaaac ggcaattttg tggattaagg tcgcggcagc
    27961 ggagcaacat atcttagttt atcaatataa taaggagttt aggatgaaag tcgcagtcct
    28021 cggcgctgct ggcggtattg gccaggcgct tgcactactg ttaaaaaccc aactgccttc
    28081 aggttcagaa ctctctctgt atgatatcgc tccagtgact cccggtgtgg ccgtcgatct
    28141 gagccatatc cctactgctg tgaaaatcaa aggtttttct ggtgaagatg cgactccggc
    28201 gctggaaggc gcagatgtcg ttcttatctc tgcaggcgta gcgcgtaaac cgggtatgga
    28261 tcgttccgac ctgtttaacg ttaacgccgg catcgtgaaa aacctggtac agcaagttgc
    28321 gaaaaactgc ccgaaagcgt gcattggtat tatcactaac ccggttaaca ccacagttgc
    28381 aattgctgct gaagtgctga aaaaagccgg tgtttatgac aaaaacaaac tgttcggcgt
    28441 taccacgctg gatatcattc gttccaacac ctttgttgcg gaactgaaag gcaaacagcc
    28501 aggcgaagtt gaagtgccgg ttattggcgg tcactctggt gttaccattc tgccgctgct
    28561 gtcacaggtt cctggcgtta gttttaccga gcaggaagtg gctgatctga ccaaacgtat
    28621 ccagaacgcg ggtactgagg tggttgaagc gaaagccggt ggcgggtctg caaccctgtc
    28681 tatgggccag gcagctgcac gttttggtct gtctctggtt cgtgcactgc agggcgaaca
    28741 aggcgttgtc gaatgtgcct acgttgaagg cgacggtcag tacgcccgtt tcttctctca
    28801 accgctgctg ctgggtaaaa acggcgtgga agagcgtaaa tctatcggta ccctgagcgc
    28861 atttgaacag aacgcgctgg aaggtatgct ggatacgctc aagaaagata tcgccctggg
    28921 caaagagttc gttaataagt aattgattag cgaataataa aaaaccggag cacagactcc
    28981 ggtttttgtt ttgagcactc gacttaattg gttgccggat attcctgaat ggtgacctgc
    29041 agcgttaact gcttatcatc acgcatcact acaaccggga tcaccgaacc agggcgaatt
    29101 tccgccacct gatccatcgt ctccagagca gagatggccg gtttgttatc caccgaaata
    29161 atcagatcgt tgacctgaat acccgcattc gccgctgggc cgtcaggtga cacttcatta
    29221 accacgatcc cttgcagttg atctatacca ccgctctgcg cgtgcagtgg tgcgatctcg
    29281 cgtccgccga taccaatgta gccgcggatc acgcgaccat cgcggatcag cttatccata
    29341 attttggttg ctaactggaa aggaatcgca aagccgatac cttccggcgt ttcgccatcg
    29401 ttactcttat caaacgacag cgtgttaatg cccatcagtt ctcccagcga gttcaccagc
    29461 gcgccgccag agttaccgtg gttaatggaa gcatcggttt gtaggaagtt ttgccgcccg
    29521 gtcgggttca gaccgattcg acccgtggca ctaataatcc cttgggtaat ggtctgcccg
    29581 aggttgtacg ggttaccgat cgccagtact acgtcgccaa tgtgcggtac gcgacgtgca
    29641 ttaattggaa tggtaggtaa accgccagtg gcattaattt taagtaccgc cagatcggtt
    29701 agagagtcag atcccaccag caatgcttca aatacacgtc catcctgtaa ggcgacgatg
    29761 atctgatcgg cgtcgttgat gacgtgttta ttggtgatga tataaccgcg ttgatccatg
    29821 attacaccgg atcccagggt gcggatctca agctggttgt gagagttggt gttcaaacca
    29881 cggttgtaaa cgttaaccac cgctggcgcg gcgcggcgaa ccgccagatt atagctggca
    29941 ggcgtctcat cggtactgtc aaattgcgga gtggaaagcg ggttaaggct gcgcagcgaa
    30001 ggcatggcaa ccagcagaat agcgccgaca attaatccaa tcgcaacgga acgtaagagc
    30061 ttcacaaaca tgatggaggc gtcattaaaa aagggaacgg cagcagcata ccacgagtta
    30121 accggacatc acacgtcagc ctgatgcccg gtttacgaca ttaacgcatc agcagataga
    30181 tgctttcatt gccgcgtaca atttgcaggg cgatgatggc cggttttgcc gccagcactt
    30241 tacgcatttc agcaatcgag ttcacccgat cgcggttgac gccaatgatc acatcgtctt
    30301 tttgcaagcc agcctgagca gctgggcttc ctttgacaac ttcatctatc ttaatacctt
    30361 tgccgccatc ttttagctga ccatcgctca acgttgcacc ttccagcgct ggccgacgaa
    30421 gaggtgctgg tatcaagcgt cacttctact tccagtggtt tgccgttacg cagcaggcca
    30481 agcttcactt tcgtgcccgg ctctgtagtc gcgatacgag agcgcaactc agcaaagcta
    30541 ttcagcggtt tgccgttgag gctggtaata atatcgcccg ctttgacgcc cgctttcgct
    30601 gagccagaac ttggcaacac ttcgctgaca aacgcgccac gctgcacgtc aaggttgaag
    30661 gctttggcga tatcggcact catctcggtg cctttgatgc ctaacaaacc gcgtttgatt
    30721 tcaccaaagt cgataagctg ctgcgccagt gttcgcgcca tattactggg gatggcaaat
    30781 ccaatcccga cgctcccgcc gccaggcgca aggattgcag tgttgatgcc aattaactca
    30841 ccgttaaggt ttaatagtgc accgccggag ttaccgcggt taatggaagc atctgtctgg
    30901 ataaagtttt ccagaccttc aagattcaac ccgctgcggc ctaatgcgga aacaatgcca
    30961 gaggtggcgg tttgcccaag gccaaatggg ttaccgaccg ctacggcaaa atcaccgacg
    31021 cgcaatttat cggagtcggc aatagcgatt tgcgttaatt tgctcgggtt ttgaatttgt
    31081 aacagggcga tatcgctctg gtcatcgcta ccaatcagtt ttgcatcaaa ctcgcgccca
    31141 tcattgagct gaatactgat tttctgtgcc tgattaatca catggttgtt ggtcagcaca
    31201 tagcctttac tggcgttgat gatgacaccg gaacctaaac cttcgaaggg ttgtgcaggt
    31261 tgatccggta aatcatcacc aaaaaacttt ttgaattctt ccgggatttt ctgtccctga
    31321 ctggccgttc cttccacccg tacgctcacc actgccggaa gcactttttc cagcattgga
    31381 gccagactgg ggagaggggc ctgatcggca acctggcctg gaatcgacgc gacggcctga
    31441 aatgacgccg agagagttaa cccggcactt aacgctaatg cactcaacag ctgggtttgt
    31501 tttttcatta ttcctgctct cgtacctgaa tgataagaaa agagattcaa aacagtttga
    31561 tgttattgaa ttttcagacg gctaacaatg agacaccaga ttaaatgata gtcggggagg
    31621 gagagaagag gagggcgcaa tggctgcgcc cgaaaaataa attagtcgcg cttcgcgcca
    31681 gtacgcagca ggccggatgc gccttcagaa tagtcgcgag gcacctgcac cggtgcctga
    31741 tcgttgctgg cttcagactc tgccagacga ttacggaacg ggtttgcttc agcagacagt
    31801 tccggcagca ggctgctgga gctttttgcc atgtgctgat acagctggcg atagtcgtgc
    31861 gccatggtat ccagtaattc cgcgctgcgg gcaaagtggc taaccagctc ttcgcgatac
    31921 tcgtccagtt cagctttatt cttttccagt tcgtactgca acgcctgttg ctggcgtagt
    31981 ttacgattac caaaacgcat ggccacagca ccaataatga tgccgacgac taacccaatt
    32041 agcgcatatt cccaggtcat gaacatctcc cgttgtcttt tgtttccgta gggtgttggc
    32101 ttcaggctcc atgcctgcgg ctgattatgc cactataacc gttaattcca cagaagtgga
    32161 atcccgactg catatcgcgt agtgtagaac ggcctttttt tcgtcaaccg tgaacaacgg
    32221 cgcaccgatt attcaaggaa taacaataag atcatgcaaa gcgttacccc aacatcgcaa
    32281 tacctgaagg cgctcaatga aggcagccat caacccgacg acgttcaaaa agaggccgtc
    32341 agccgcctgg aaattattta tcaagaactc atcaatagca ggccaccagc ccccaggacg
    32401 agtgggctaa tggcgcgggt cggtaagctg tggggtaaac gcgaagacac aaagcatacg
    32461 ccagtgcgtg gcttatatat gtggggcggt gtaggacgcg ggaaaacctg gctgatggac
    32521 cttttctatc aaagcctgcc gggagagcgg aaacagcgcc tgcactttca ccgttttatg
    32581 ctgcgggtgc acgaagagct aactgcctta caggggcaga ccgatccgct ggaaattatt
    32641 gccgatcgct ttaaagccga aactgacgtg ctctgttttg acgaattttt tgtttctgat
    32701 attaccgacg ccatgctact tggcggtctg atgaaagccc tgttcacccg cggtattacc
    32761 ctggtagcga cgtcaaatat tccgccggac gaactttatc gaaatggcct gcaacgtgcg
    32821 cgttttctgc ctgcaatcga tgccattaaa cagcattgtg atgtaatgaa cgtggacgct
    32881 ggtgttgatt atcgactgcg tacactcact caggcgcatc tgtggctttc gccactcaac
    32941 gatgaaaccc gggcgcagat ggataaacta tggttggcgc tggcgggggt gaaacgagaa
    33001 aattcaccga cgttagaaat caaccatcgg ccattggcga caatgggcgt cgagaaccag
    33061 acgctggcgg tttcttttac tacgctgtgc gtcgacgccc gcagtcagca tgactatatt
    33121 gcgctctcac gcctgtttca cacagtaatg ttgtttgatg tgccagtcat gacgcggttg
    33181 atggagagcg aagcgcggcg ctttattgcg ctggtggatg agttttacga gcgccatgtc
    33241 aaattagtgg tgagtgcaga agtgccgctg tatgaaattt atcagggtga gcggctgaag
    33301 tttgagttcc agcgttgcct gtcacgtctg caagagatgc aaagcgaaga gtatctgaag
    33361 cgcgagcatt tggcgggtta aaacctgcca caaatcacaa aaaggggtcg atctttgacc
    33421 ccgacttctc tataatcctg cgaccccacg ttacaagaaa gtttttttcc caaaactttt
    33481 tgtgtgctgg cataggctat tcgaaggggt aggtttgccg gactttgtcg tgtgaacctc
    33541 aacaattgaa gacgtttggg tgttcaccaa cgtgtaacta tttattgggt aagcttttaa
    33601 tgaaaacttt tacagctaaa ccagaaaccg taaaacgcga ctggtatgtt gttgacgcga
    33661 ccggtaaaac tctgggccgt ctggctactg aactggctcg tcgcctgcgc ggtaagcaca
    33721 aagcggaata tactccgcac gtagataccg gtgattacat catcgttctg aacgctgaca
    33781 aagttgctgt aaccggcaac aagcgtactg acaaagtgta ctatcaccac accggccaca
    33841 tcggtggtat caaacaagcg acctttgaag agatgattgc tcgccgtcct gagcgtgtga
    33901 ttgaaatcgc ggttaaaggc atgttgccaa aaggcccgct gggtcgtgct atgttccgta
    33961 aactgaaagt ttacgcgggt aacgagcaca accacgcggc acagcaaccg caagttcttg
    34021 acatctaatc gggattatag gcaatggctg aaaatcaata ctacggcact ggtcgccgca
    34081 aaagttccgc agctcgcgtt ttcatcaaac cgggcaacgg taaaatcgta atcaaccaac
    34141 gttctctgga acagtacttc ggtcgtgaaa ctgcccgcat ggtagttcgt cagccgctgg
    34201 aactggtcga catggttgag aaactggacc tgtacatcac cgttaaaggt ggtggtatct
    34261 ctggtcaggc tggtgcgatc cgtcacggta tcacccgcgc tctgatggaa tacgacgagt
    34321 ccctgcgttc tgaactgcgt aaagctggct tcgttactcg tgacgctcgt caggttgaac
    34381 gtaagaaagt cggtctgcgt aaagcacgtc gtcgtccgca gttctccaaa cgttaattgg
    34441 cttctgctac ggcagaaaac aatttgcgaa aaaacccgct tcggcgggtt tttttatggc
    34501 taaattctga atcagcgtaa aaaatgggaa gttgcttttt gctgccgtct gaaagacagg
    34561 taaaacaaaa accacatcgc caataagggt ctaagtcaac tatttcagac taaagcgcat
    34621 ctctttttcc ccatttccgg catccactca ccacaaaggt cgcaaaatct ggtaaactat
    34681 catccaattt tctgcccaaa tgtcgggtat tgctcatttt ttgtttgatt ttcgaacaaa
    34741 gagagtcgtt ccttattggg taacacaact tctgactggc cacctggtgg ctggtagcag
    34801 taaaaattct gactatacct ggaggttttc atggctgtcg ctgccaacaa acgttcggta
    34861 atgacgctgt tttccggtcc tactgacatc tatagccatc aggtccgcat tgtgctggct
    34921 gagaaaggtg taagtttcga gatcgaacac gtggaaaagg acaatccgcc tcaggatctg
    34981 attgacctca acccgaatca gagcgttccg accctggtgg atcgtgagct gaccctgtgg
    35041 gaatctcgca tcattatgga atatctggat gagcgtttcc cgcatccgcc actgatgcct
    35101 gtttacccgg tagctcgcgg tgaaagccgt ctgtacatgc atcgcatcga aaaagactgg
    35161 tacacgctga tgaacactat catcaacggt tcagcttctg aagcagatgc cgcacgtaag
    35221 caactgcgcg aagaactgct ggcgattgcg ccggtcttcg gtcagaagcc gtacttcctg
    35281 agcgatgagt tcagcctggt cgattgctac cttgctccgc tgctgtggcg tctgccgcaa
    35341 ctgggcatcg agttcagcgg cccgggtgcg aaagagctga aaggctatat gacccgcgtc
    35401 tttgagcgtg actctttcct tgcttcttta actgaagcag aacgtgaaat gcgtctgggc
    35461 cggagttaat ctgtatggat ttgtcacagc taacaccacg tcgtccctat ctgctgcgtg
    35521 cattctatga gtggttgctg gataaccagc tcacgccgca cctggtggtg gatgtgacgc
    35581 tccctggcgt gcaggttcct atggaatatg cgcgtgacgg gcaaatcgta ctcaacattg
    35641 cgccgcgtgc tgtcggcaat ctggaactgg cgaatgatga ggtgcgcttt aacgcgcgct
    35701 ttggtggcat tccgcgtcag gtttctgtgc cgctggctgc cgtgctggct atctacgccc
    35761 gtgaaaatgg cgcaggcacg atgtttgagc ctgaagctgc ctacgatgaa gataccagca
    35821 tcatgaatga tgaagaggca tcggcagaca acgaaaccgt tatgtcggtt attgatggcg
    35881 acaagccaga tcacgatgat gacactcatc ctgacgatga acctccgcag ccaccacgcg
    35941 gtggtcgacc ggcattacgc gttgtgaagt aatacaaaac aggcccaggc ggcctgtttt
    36001 gtctttttaa tgacttaata aaatagcgtg attgtggcaa tcatattcac cacgaaaccg
    36061 actgccatgg ggatcgctgt ccgcttcacc accgcaaaag gcgaaacgcc tgcaattcct
    36121 gacgtaacga ccaccaccgc agtaatcggc gaaaccgcgc gcgctagcgt cgtggcaaaa
    36181 tgcatcggca taatcattac aaccgctggt acatgtagtc cggctgcgat attcggaata
    36241 agactggcaa atggcataaa cggcgcattg ccagagccca tcacaatggc acaaatggca
    36301 atgactagcg ccataataat catcacgcca ataccgccca gaccagaatg ctccgcaccc
    36361 ctgataaccg catcaacagt gccaatcgtc gttaagcctt tcgcaaatat ttcacccgcg
    36421 accaccagcg ttaccacgtt gacaaactgc gtacccatgc cgtcaaaaaa cgcctgcaca
    36481 tcgtccattg tttcgcgcaa gttatgcttg cggaagaact caacaaacat cgtcaccgtc
    36541 aaacttagta acatcaccac caccagatgc agttctgact gcatcagccc gacgtgggcg
    36601 aggaacagcg agccgagcat caggattaac ggcatcacag gtaaaatggc gtaatagagc
    36661 ggcgggacat tatcgagagt tttttgctct gcctgttcgt gattgatatt tttatctttt
    36721 ttgtcaaaag cgcgttgcac gaaaaagtgg gagatcgcca ccgagataat gacgcaagag
    36781 gcgaccggaa gctggtagtg gaagaagtat gtggcaattt tcattcccgc gacctgggct
    36841 gcaaaaatgg agttcgtttc cagaatcccc cactcaatgg acatcgtggt tgcgataacc
    36901 gccaccgcag agaggcgact tactcccaga ctcaccagcg tcggaaataa ggtgaccatc
    36961 agcaacatac ccagaccgga ggcgctggta ataaactgcg ccatgatttg gccgatgacg
    37021 taagttgccg acagaataat atacggcgag cgaatgagtt ttaacgggcg gcttagcaag
    37081 ctcaccatcg cgcaactggc tcctatgcgc tccatgtagc gggcataacc gcccaccgcc
    37141 ataatcgaca gccccaatcc ggcaatgcgg ttggacagca tgcgcaggat ttcattataa
    37201 atgtcgacga tcaagtagcc gctgcttttg gcgggatcga gtacaccacc gaaccctaac
    37261 cagactccgc acatcatcag gaagacacct gcggcagcca gcaccacctg aggtttgtag
    37321 tttttcagga tcaaatagcc catcgtaatt aatacgat
//
LOCUS       DAJTPG010000030        36070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTPG010000030 DAJTPG010000000
VERSION     DAJTPG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..884)
                     /locus_tag="OO230_001596"
     CDS             complement(<1..884)
                     /locus_tag="OO230_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5691404.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDV"
     gene            complement(1031..1107)
                     /locus_tag="OO230_001597"
     tRNA            complement(1031..1107)
                     /locus_tag="OO230_001597"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1071..1073),aa:Pro,seq:tgg)
     gene            complement(1150..1236)
                     /locus_tag="OO230_001598"
     tRNA            complement(1150..1236)
                     /locus_tag="OO230_001598"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1200..1202),aa:Leu,seq:cag)
     gene            complement(1257..1332)
                     /locus_tag="OO230_001599"
     tRNA            complement(1257..1332)
                     /locus_tag="OO230_001599"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1297..1299),aa:His,seq:gtg)
     gene            complement(1391..1467)
                     /locus_tag="OO230_001600"
     tRNA            complement(1391..1467)
                     /locus_tag="OO230_001600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Arg,seq:ccg)
     gene            complement(1570..2955)
                     /locus_tag="OO230_001601"
     CDS             complement(1570..2955)
                     /locus_tag="OO230_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5691405.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3146..3886)
                     /gene="rffM"
                     /locus_tag="OO230_001602"
     CDS             complement(3146..3886)
                     /gene="rffM"
                     /locus_tag="OO230_001602"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5691406.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(3889..5241)
                     /gene="wzyE"
                     /locus_tag="OO230_001603"
     CDS             complement(3889..5241)
                     /gene="wzyE"
                     /locus_tag="OO230_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5691407.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5238..6317)
                     /gene="rffT"
                     /locus_tag="OO230_001604"
                     /gene_synonym="wecF"
     CDS             complement(5238..6317)
                     /gene="rffT"
                     /locus_tag="OO230_001604"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5691408.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6314..7564)
                     /gene="wzxE"
                     /locus_tag="OO230_001605"
     CDS             complement(6314..7564)
                     /gene="wzxE"
                     /locus_tag="OO230_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5691409.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7566..8696)
                     /gene="rffA"
                     /locus_tag="OO230_001606"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7566..8696)
                     /gene="rffA"
                     /locus_tag="OO230_001606"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5691410.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(8701..9375)
                     /gene="rffC"
                     /locus_tag="OO230_001607"
                     /gene_synonym="wecD"
     CDS             complement(8701..9375)
                     /gene="rffC"
                     /locus_tag="OO230_001607"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5691411.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9353..10234)
                     /gene="rfbA"
                     /locus_tag="OO230_001608"
     CDS             complement(9353..10234)
                     /gene="rfbA"
                     /locus_tag="OO230_001608"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5691412.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10253..11320)
                     /gene="rffG"
                     /locus_tag="OO230_001609"
     CDS             complement(10253..11320)
                     /gene="rffG"
                     /locus_tag="OO230_001609"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5691413.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11320..12582)
                     /gene="wecC"
                     /locus_tag="OO230_001610"
     CDS             complement(11320..12582)
                     /gene="wecC"
                     /locus_tag="OO230_001610"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5691414.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12579..13709)
                     /gene="wecB"
                     /locus_tag="OO230_001611"
     CDS             complement(12579..13709)
                     /gene="wecB"
                     /locus_tag="OO230_001611"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5691415.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(13765..14811)
                     /gene="wzzE"
                     /locus_tag="OO230_001612"
     CDS             complement(13765..14811)
                     /gene="wzzE"
                     /locus_tag="OO230_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5691416.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(14823..15926)
                     /gene="wecA"
                     /locus_tag="OO230_001613"
     CDS             complement(14823..15926)
                     /gene="wecA"
                     /locus_tag="OO230_001613"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5691417.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16166..17425)
                     /gene="rho"
                     /locus_tag="OO230_001614"
     CDS             complement(16166..17425)
                     /gene="rho"
                     /locus_tag="OO230_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5691418.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17510..17611)
                     /gene="rhoL"
                     /locus_tag="OO230_001615"
     CDS             complement(17510..17611)
                     /gene="rhoL"
                     /locus_tag="OO230_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5691419.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(17752..18081)
                     /gene="trxA"
                     /locus_tag="OO230_001616"
     CDS             complement(17752..18081)
                     /gene="trxA"
                     /locus_tag="OO230_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5691420.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18212..19477
                     /gene="rhlB"
                     /locus_tag="OO230_001617"
     CDS             18212..19477
                     /gene="rhlB"
                     /locus_tag="OO230_001617"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5691421.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19611..21095
                     /gene="gppA"
                     /locus_tag="OO230_001618"
     CDS             19611..21095
                     /gene="gppA"
                     /locus_tag="OO230_001618"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5691422.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21142..23163)
                     /gene="rep"
                     /locus_tag="OO230_001619"
     CDS             complement(21142..23163)
                     /gene="rep"
                     /locus_tag="OO230_001619"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5691423.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23250..23531
                     /gene="ppiC"
                     /locus_tag="OO230_001620"
     CDS             23250..23531
                     /gene="ppiC"
                     /locus_tag="OO230_001620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5691424.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23577..25052)
                     /gene="ilvC"
                     /locus_tag="OO230_001621"
     CDS             complement(23577..25052)
                     /gene="ilvC"
                     /locus_tag="OO230_001621"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5691425.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25202..26092
                     /gene="ilvY"
                     /locus_tag="OO230_001622"
     CDS             25202..26092
                     /gene="ilvY"
                     /locus_tag="OO230_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5691426.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26089..27633)
                     /gene="ilvA"
                     /locus_tag="OO230_001623"
     CDS             complement(26089..27633)
                     /gene="ilvA"
                     /locus_tag="OO230_001623"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5691427.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27636..29486)
                     /gene="ilvD"
                     /locus_tag="OO230_001624"
     CDS             complement(27636..29486)
                     /gene="ilvD"
                     /locus_tag="OO230_001624"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5691428.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(29551..30480)
                     /gene="ilvE"
                     /locus_tag="OO230_001625"
     CDS             complement(29551..30480)
                     /gene="ilvE"
                     /locus_tag="OO230_001625"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5691429.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30500..30763)
                     /gene="ilvM"
                     /locus_tag="OO230_001626"
     CDS             complement(30500..30763)
                     /gene="ilvM"
                     /locus_tag="OO230_001626"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5691430.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30760..32406)
                     /locus_tag="OO230_001627"
     CDS             complement(30760..32406)
                     /locus_tag="OO230_001627"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5691431.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32409..32459)
                     /locus_tag="OO230_001628"
     CDS             complement(32409..32459)
                     /locus_tag="OO230_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691432.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32545..32643)
                     /gene="ilvL"
                     /locus_tag="OO230_001629"
     CDS             complement(32545..32643)
                     /gene="ilvL"
                     /locus_tag="OO230_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5691433.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32995..34474
                     /locus_tag="OO230_001630"
                     /pseudo
     CDS             32995..34474
                     /locus_tag="OO230_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(34499..34837)
                     /gene="maoP"
                     /locus_tag="OO230_001631"
     CDS             complement(34499..34837)
                     /gene="maoP"
                     /locus_tag="OO230_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5691434.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34956..35795
                     /gene="hdfR"
                     /locus_tag="OO230_001632"
     CDS             34956..35795
                     /gene="hdfR"
                     /locus_tag="OO230_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5691435.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(35891..35966)
                     /locus_tag="OO230_001633"
     tRNA            complement(35891..35966)
                     /locus_tag="OO230_001633"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35931..35933),aa:Trp,seq:cca)
     gene            complement(35975..36051)
                     /locus_tag="OO230_001634"
     tRNA            complement(35975..36051)
                     /locus_tag="OO230_001634"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36015..36017),aa:Asp,seq:gtc)
BASE COUNT         8812 a         9870 c         9096 g         8292 t
ORIGIN      
        1 taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac aggttggctg atgatggcaa
       61 accgcacccg gcgcaccgac aaaattggca aacgcccatt cgatattcat cacgaaaatc
      121 ttgccgacgt cgcgtttgat ccagtggtca aaaatcgcca ccagaaattc accaaattca
      181 tcggggcgca cagaccattc cgtcagttcc ccctgaatat caccaggcgc atgtagtttc
      241 agtccatcgc ggacagctgt ttcatcagcc aggcgctcga ccaccggaat aaactggatg
      301 aattcgactc ccgcatcgca caaaaaatca taaacctgta acggttgctg cgcgctggtg
      361 cggttgacgc agaccagcac gttatagtcg acatgatgtt tttgcaggag cgtcagggca
      421 cgcatcacca gtttatgcgt gggtctgccg cctttggtca cgcgatattg attgtggatc
      481 tcagccggac catccagcga taacccaaca agaaaattat tttctgccag aaatgcacac
      541 cattcgtcat cgagcagcac gccgttagtc tggaaactgt tacttatctt cctgccagca
      601 ccatatttcg cctgtagcgc cacggcacgg cggtaaaact ccagcccgag tagcgttggt
      661 tcgccgccct gccaggtaaa agccacttcg ttttgcagtt cgctggcggc gatatagtga
      721 cggatatacg cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg
      781 gattgttttt cgagataaaa acagtagtca cagttcagat tacaatcgga accgctcggt
      841 ttcgctatca catgaaaagc acgcgttgga acctgttgca gcatggtcgc tccttgcctg
      901 aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag ttattacggg
      961 cgtttattgc gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg
     1021 ggttcaaaat tggtcggcga gagaggattc gaacctccga cccactggtc ccaaaccagt
     1081 tgcgctacca agctgcgcta ctcgccgaaa tactgctttt tgaattttta gttcaattct
     1141 ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg tccgtaagga cactaacacc
     1201 tgaagctagc gcgtctacca attccgccac cttcgcattg tcacaacttc taataatggg
     1261 gtggctaatg ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg
     1321 agctatagcc accactacaa atcttgttac gcggtattac taccaccgca gctcaagcgc
     1381 tgggactaaa tggcgcgccc gacaggattc gaacctgaga cctctgcctc cggagggcag
     1441 cgctctatcc agctgagcta cgggcgctta gcgccgttgc gggggtggat aatacggact
     1501 tcccacccct ctgtctagtc cctttttaaa taaattgctc gtttggttac ggtttgtgcg
     1561 ttttgctgct tattcctcca gtttatgcgc tttaccgtgg cgattaagcc caaaaacttt
     1621 ataaatcgcc gtcaccgcca gcataaagat gatgccgaca aacagcgaca tacgcgtatc
     1681 ttcattaaag tacatgccga tcaaaacgca aatcaggaat gccattgtta cgtagttggc
     1741 ccacgggaac aggattgagc ggaacggatg gctggcaatc gccgctttat gtgcacgccg
     1801 aaaacgcagc tggcttatca atatcacaaa ccatggcacc atccccggaa gcacgctggc
     1861 actgtagacg tagacaaaca cacgctgcgg attgggaatg atgtagttca ggcatgagcc
     1921 aattagcaga atagcaatag atactgccac acccgcaacc ggtacgccgt gacgggaaac
     1981 ttttgccatt gccgccggta actgacggtt tttcgccagt gcgtagagca tacgtccgca
     2041 actgtacatg ccgctgttac agccagagag cgcagccgtc agcaccacaa agttgataat
     2101 gcccgccgct gcggtaatac cgattttggc aaaagtcagt acgaacgggc tgccgttgct
     2161 gccaatttca ttccacggga agatggtgac gataacgaaa atcgcgccta cgtagaaaat
     2221 caggatccgc cacagcactt tgcctacggc actgcgcagc gtcacctgcg gattcttcgc
     2281 ttcaccggca gtaatgccaa tcagctccac gccctggtag gacgccacca caatacacag
     2341 agcggtcagg aaccctttcc agccacccgc aaagaaaccg ccatgctctg tgagattgct
     2401 aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa atcacgccca agccaatgac
     2461 aatcatcacg ataatcgtgg tgactttgat catcgcgaac cagaactcga tttcgccgta
     2521 caaccgtacc gccgccagat tcgccaacgc caccagcgcc accgcgatca atgcgggtat
     2581 ccactgcgcc atctccgggg accaaaactg gacataaacg ccaatggcgg tgatttctga
     2641 gatccccacc gccatccaca taaaccagta agaccaggcg gtgagatagc caaaaaacgg
     2701 gctcatataa cgatgcgcat aaacggcgaa cgaaccggta accggttcga ggaacaacat
     2761 ttcgcccatt gaacgcatga tgaaaaaaac gaacagcccg gcgatgatat aggccaacaa
     2821 tacggatggc ccggcccatt tcagggtact ggcggccccc ataaacaggc cgacgccaat
     2881 ggtgcccccc agggcgatga gttcgatatg tcgagcttcc agcccacgct gtagctctgg
     2941 tttgttatct gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt taccggttat
     3001 cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa
     3061 gcattaaaaa tttgaatgct ttgtgtaata aaaaagcaga caggcgacga agtgaccact
     3121 ccgtcgcttt acaaagagag gaaaatcaga ggttgccggt gtagtgccag cgtaaataac
     3181 gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg tagagccact
     3241 ccagtcccag cgtttgccag attttcggtg cgcgttttac gtgaccggtg aaaacatcgt
     3301 aagtcccgcc aacgcccata tacagcgcat ctggatgcac cagacggcag tcgcgcatga
     3361 agatctcctg ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa
     3421 tgcgttcaaa cagcgcctga cgctgctcgg gtttaaaata accatcctga ctgccaacga
     3481 tattcacatt ccattggttg cgcagtttag cttcagtttg cgccagcact tcaggtttac
     3541 cgcccacaag aaataccggc gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga
     3601 gatcggcacc ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt actgaacgta
     3661 caatgctgat gccatccgca tatttaaatt cggcagcgtt aattaactcc ctgacctcgg
     3721 cgttatcttc aatggtcagc attttttcgg cattaatggc aaccagcgtt ccctgcttaa
     3781 gctgcccgtc agcaaacaga taatcgaggg catgctgcat atcacgccaa ccaatcaact
     3841 gtaagccacg cagcgtatag gtcggtgccg tggtgttgtt attcattgtt atccttcaac
     3901 ctgcgtccgg agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca
     3961 gtacaacagt tttgcgatca tcagacatgc gccgaagacc acgataaaaa agactacgcg
     4021 tgagacaaac gaatccagcc cttcacgtgc cagcacgatc atattgaaga tcgccccaaa
     4081 gcagaaactg tgcaatatcg cagccttata gcgattagtc tcgcggttgc ccagctcata
     4141 cagccagtcg aaccatttga tgatcagtcc aaccacaatc gccccgagtg ggatgaacaa
     4201 cgcgccgccc atcaccacca gtgagcctat aagcgtaggc gagatcgcca gtccggagtg
     4261 gttattcagt acttcccagg taaagtaatt ggctgagttc agcaccatac tcgggcgccc
     4321 cggccacagc caggaaggga taaagacata gaaatcgcgg acaattggag ccaggccctg
     4381 gaagtcgatg ttgtcgtagt tctgcaacag caacgccaga ttctcccacg gtgaaaaggt
     4441 gtcgcgagtg agatagagaa acgtatagaa cgcttcatcg ccgctcacat tcattccata
     4501 gcgttttaac gccagccaga acatgccaac aatccccagc acgcccgccg ccgccagcat
     4561 ccacaacgaa atccagccgc gaataatgcc aataaacagg aagatagcga atgcgatgat
     4621 gatattggcg cgagtgccgc cgacaatcat ataagtcagc aagccaaagg cgaccgtgct
     4681 gacgaggaaa aacagccacg ctttgctgtc ctggcgcaga aagtagacca ccagcatcgc
     4741 cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg gagacttcac tggaaaagat
     4801 ctgactgtag gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga agatgccgac
     4861 gcttaccagc gcgataccca tcaggatcac ccacgtaaga ttggtctcca cgcggttcat
     4921 ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt
     4981 gacatagtaa accgcgtaga agcagcccgc agaaagcaac acctgcaaca agatttctgg
     5041 cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg gtcagcggga agccgaagaa
     5101 aaaggtgagc aaaaacagca atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa
     5161 ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc agccagacca caaacaggcc
     5221 actgaattgc agcagactca tgcgacctcc ccggcggcaa tcgccaacgc ccgctgccag
     5281 ccttgtagat agttagggct aaagaaggca atggtgtttt tatccgccga cgccaactgg
     5341 cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg tagtaaacag cactggcaaa
     5401 tgctgttccg tcatatcctg ccagaacgga ttttccctgt taagcacaca aggaatgccc
     5461 gcctgaatca gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca
     5521 agatcgcact gacgaagtag cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt
     5581 tgcagatttt cttcgctgaa taactccagc cccgcctgac gaacttcctc aatgtacgct
     5641 tcgttattag gcggatatcc catcggcacc accactttta ccgtatcgcc aaattgctga
     5701 tgaacggcac gtaaagcggc gacatgctca ttgctgcggt cgccggagtt ccccaccaga
     5761 atggtcattt ttccttcacg ttgccgatcg ttcgccatcg tattgagaga agggtccatc
     5821 cgcgtcggga agtacagcag ttctccccgc acctttgggt gtgttttggc aaaaaagctc
     5881 aaatcgccgc gggtggcaaa tacacagccg actcgctttt gcgccaggcg acgtagtggg
     5941 taaaaaagct tatatctcaa gccactggaa agctcataca ggtctgcccc ccagatatgc
     6001 cagaaaaact ggctgggctt aatgccacca ctcagcagag ccagccacag tgtgggattg
     6061 aactgaccgt ggaaaaaaaa tcgctgctga cggttagctt ttgctttcgc gatgaccgct
     6121 tccgccagcg attttttccc agggaaaaat tgcacagaaa gcgccggaca gctatcactt
     6181 aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt cgcggccagc
     6241 gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag ggatatccga tcccagtacg
     6301 tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa
     6361 aatagacgat ataagttgcc atatatgcct gcgccgcgcc cagtgcacca tgcgcaggga
     6421 ttagccagtg ggcaaatacc atcaataaag tgaactggct gacttccgcc agaatataaa
     6481 accgcagtga cgctttggcg atcaccagat aaccaaagac ataagcgccc acttttaaca
     6541 catcacccac taactgccag gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca
     6601 gcaaccagat agcaaaatca cgcagcaacc agacggtaaa actcgctgcc gccactgccg
     6661 gtaagacgaa tttcagcgat ttaaccactt cccgggtgat atcgcgcttt tccgttagcc
     6721 gcgacaacgt gggcagcaaa tatacgctga acgatgccgt aataaattgc aggtaggcat
     6781 cggaaatact gctcacccct tgccagatcc ccacttcatc ccagctatac tgcgccgcca
     6841 gcagtttacg catcatgatg taagcaacag gcaaggtcac cgacgtaatc aacgccatga
     6901 gcgtaaattt gctcaactgc cctgccagac cgttatccca gctgggtttc agatagctta
     6961 acgggatgac accacgttta atcaacatga tggcggcagg aattaccacc agcgcgggaa
     7021 tcagcgccag acccagcaac gccccttcat aaccgcccaa acggtaactg acgtagtacg
     7081 cgagaacgcc aatcaagctg ccgacaatca gcgataacgc attacctgcg gcatcgcgaa
     7141 agcctttcat cagcgccagt aacaggttgc cccaggcgat ccccatttgc accagcgcca
     7201 ctaaacgcac cagcccctga tagtcggtat taccaaacaa tccctggctg attggcgcag
     7261 ctgccagcac aaaaaccagc gccatcagcg tagagaaacc aagtaccatc gctgatgaag
     7321 tgccgaccac gcggcgcagc tgttgcggat tatcatggta ctgggcaacg tatttggtta
     7381 caccgttaaa gatgccagcc ccggcaagca cgccgagcac ggtaatcaac tggcggaaat
     7441 ttgccgccag cccaagcccc gccggaccaa atgacaccgc cagcaactta ccgaccagta
     7501 acccggcacc aatcttgacc agtgtactgg ccgccgtcca caaggacgct tttgccaacg
     7561 acatatcagg agaagtagtt caacaaagtc gcaattaccg tacgctgatt gacgggcgac
     7621 aggttgtaga acagcggcag gcgcagcagg cgctcgctct ctttggtggt gtagcgatct
     7681 tcaccgtgga actcaccaaa gtgttccccc gcagggcaac cgtgcagcgg aatgtaatga
     7741 aacaccgcca tgatttccgc ttctttcaga aagttaatca acgcgctccg gtcatcaata
     7801 tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc agccatcggg aatcgacggc
     7861 agctcgatac gcccggcttt cgccagaggc gctaacgtat cgtagtagtt ttgccacagc
     7921 gccagacgtt gctggttgat acgatccgct gcttccagtt gcgcccacag gtacgcagct
     7981 tgcagatcgg acatcaaata gctggagcca atatcgcgcc aggtatattt atcgacctga
     8041 ccacggaaga actggctgcg gttagtgccc ttttcacgga tgatctcggc tcgttcgatt
     8101 aacgctttat cgttaatcag cgtcgcgccg ccttcaccac ccgccgtgta gtttttggtt
     8161 tcatggaagc taaagcagcc aatatgacca atggttccca gtgcacgccc tttgtaagtg
     8221 gacatcacgc cctgagcggc atcttccacc acaaacaaat tatgcttttt cgccaacgcc
     8281 ataatggtgt ccatttcgca ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt
     8341 ttgtcggtga tcgctgcttc aatcaacgtt tcgtcgatgt tcatggtgtc cgggcgaaca
     8401 tccacaaaga cgattttcgc gccacgcagc acaaaggcat tggcggtgga gacaaaggtg
     8461 tagctcggca tgatcacttc atcgccaggc tggatatcga gcagcagcgc cgccatctcc
     8521 agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg ttgctccagc
     8581 cactgctggc agcgacgggt aaaaccgcca tcgccacaca gtttgccgct acccattgcc
     8641 gactgcatat agtcgagttc ggttcccacc accggcggtg cgttaaatgg aatcatgtga
     8701 tcacctgtat aaccagtacg cggtgctttc tacattcgca ccactttgta tgtatcgttt
     8761 aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa gttgttttac cgcgagcata
     8821 cgcccagttt agcgccgttt gcatcagctc agcacctgca ccgcgtccag ccagcaggcc
     8881 aattcgcgca tctgtcgcat tgagttcccg taaagagaca tagccgcgaa tatcgccgga
     8941 cgccgcacgt agaatcagac attgatgatc aaaggtgccg cgcacggcat tttcaatcca
     9001 ctgtgcataa aagcgaccgc tggcgtcagg cgcataccac ggcgcacgaa aacggctttg
     9061 cgcaaatgcg gcgctagcta actgacgtaa tgcgggaata tcggtctctt gtgccacgac
     9121 agcaccgcta tcactgacat tgttcacggg tagcgccaaa tcaacttcac cttctaccag
     9181 ggagaatccc agctgttgca gggcatccag ttcccccgta tttgatgccg caattttggc
     9241 ctgcacccgt gaccatggcg ctaaggcgtc tgacgtcagg agcggtgctt cagacgtaat
     9301 gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc caggttagtg gctcaatact
     9361 ggcgcggacg ggcacgaagt aactccagca gatattggcc gtagccagtt ttcgctaatg
     9421 aactggcagc acgcttcaca ccctcgtcat cgagccagcc gttacgccag gcgatctctt
     9481 ccaggcaggc aatcttaaag ccctggcgtt tttctactgt ctgcacaaaa gtgctggcct
     9541 caatcaggct gtcgtgagtg ccggtatcca gccaggcaaa tccgcgcccg agcagttcaa
     9601 cggtcaggtt gcccgcctcg aggtacatct ggttgatgga ggtaatctcc agttcaccgc
     9661 gctccgacgg cttcacctgc tttgcgtact ccacgacttt actgtcgtag aaataaagcc
     9721 cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc ttcaagcgag atagcgcgga
     9781 aattgtcgtc aaattccacc acgccaaagc gttccgggtc catcacctga tagccaaaaa
     9841 ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt cggactgaag ccctgaccga
     9901 agaagatgtt atcgcccagc accagacaag aaggttcacc attaaggaag gtttcaccga
     9961 tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc ggcatattcc agctggatac
    10021 cgaactcact accatcgccc agcaggcgct ggaaataacc tttatcttcc ggcgtagtga
    10081 tgatgagaat ttcgcggata ccggccagca tcagcaccga cagcggatag taaatcattg
    10141 gcttatcgta aatcggcaac agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg
    10201 taccggaacc gcccgccagg ataataccct tcatgtttgc ctccgctgaa aattagcctt
    10261 tcagacctaa acgctcaccc tgatagctgc cgtcctgcac ctgcttccac cagctttcat
    10321 tagccagata ccactgaacc gttttacgca ttccactttc aaaggtttcc tgcggcagcc
    10381 agccaagttc acgggcaatt ttcgaagcat caatggcata acgcagatca tgccccggac
    10441 ggtcagcgac aaaggtgatc aggtcacgat agtacgccac gccgtgcggc ttgttcgaag
    10501 ccagttcttc cagcagctcg caaatggttt ccacaacatc gagattctta cgctcgttgt
    10561 gaccaccaat attataggtt tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc
    10621 gggcgtgatc ttccacatac agccagtcac ggatttgctg cccgttgcca tataccggca
    10681 gcggtttacc cgccagcgcg ttgaggatca tcagcgggat cagtttttcc ggaaagtggt
    10741 aaggaccgta gttattcgag cagttggtga tcagcgtcgg cagaccgtag gtccgcagcc
    10801 aggcgcgcac cagatggtcg ctgctggctt ttgacgcgga ataagggctg ctcggcgcat
    10861 acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg
    10921 tggagatatg atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta ttccagtaag
    10981 cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata aacgctgccg
    11041 ggccgtcaat agaacggtca acatggcttt cggctgccag atgcatgaca cagtctggct
    11101 gatgctcagt gaatacgcgt gccagttctg cccgatcgca gatatcaact ttctcaaagg
    11161 caaagcgctc gctttgcgcg accggtgcca gcgacatcag gtttccggcg taggtcagct
    11221 tatcgaccac taccaccgca tcgctcgttt cgttgatgat ataacgcacc agcgccgagc
    11281 caataaaccc ggcaccacct gttatcagaa tttttctcat cagcgccaga ctcctttggc
    11341 atcgacgaca tactgctgat ggacattgtc gccattgata actttgaact gactatgatc
    11401 gaccagcatc accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca
    11461 aagcccggtc agttttttcg gcaactggtg gatgttaggc tcaacaacca gcgtttcgcc
    11521 gctgtgccac tgggcgatca gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc
    11581 aatattcggt ttaaacgcca gaccaaagca ggcgattttc agttcactgg ctcgtttatc
    11641 ggtagcagca aggcagtcgg caaccgccgc tttcacctga tcgataaccc agaacggttt
    11701 gtgatcgtta acttcgcgcg cggtacggat aagccgcgcc tgctgggggt tctgtgccac
    11761 gataaaccac ggatcaacag caatgcagtg accgcccacg ccagggccag gctgaagaat
    11821 attaacgcga gggtgacgat tcgccaggcg aatcagttcc cagacgttaa tcccctgatc
    11881 ggcacaaatc agcgacaatt cattagcaaa agcgatattc acatcgcgga agctgttttc
    11941 ggtgagctta cacatttccg ccgtccgcga gttagtgacg acacactcac cttcgaggaa
    12001 aattttgtac aattcgctgg cgcgggccga acaaaccggc gtcataccac caatcacgcg
    12061 atcgttttta atcagctcga ccataacctg gcccggtaat acacgttccg ggcagtaagc
    12121 aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa gtcagatccg ggcgcatctc
    12181 tgctaaccat tctgccatct tctcggttga ccccaccggc gaggtggatt caaggatcac
    12241 cagcgcgcct tttttcagca ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat
    12301 atctggctca tgatcgcctt taaacggcgt aggtacagca atcagccagg catccgcttc
    12361 aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg gcagttttta ctacgctcgc
    12421 caaatcaggc tcgacgatat ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt
    12481 gatatcgaca ccaatcacct gtttttgccg tgaggcaaac gctgctgctg taggcagccc
    12541 gatgtaaccc agtccgataa cagaaatggt cgcaaaactc atagtgatat ccgattattt
    12601 tttaacgctt ccagaatgcg agagcatgcc tgaccatcac catacgggtt atgggcgcgg
    12661 ctcatagctt gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc
    12721 tgcttatccg tgcctaccag acgcaccgta cccgccgtca ccgcttccgg acgctcagtg
    12781 gtatcgcgca tcaccagcac cggtttcccc agcgaaggcg cttcttcctg aatgccgcct
    12841 gagtcagtca aaatcagcca ggcgtggttc atcagccaga caaacggtaa atactcctgg
    12901 ggatcgatca gaatgacatt tttcacatgc cccagaatgc gattgaccgg ctctctgacg
    12961 ttcgggttga gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt ggcgatgtct
    13021 gccagcgcgt ggcagatttc ttcaaagcca cgaccgaaac tctcacgcct atgaccggtc
    13081 accagaatca tctttttatc gggggcgata aacgggtaat ttgccgccag ttctgaacgc
    13141 agcgtgtcgc tgctcatcac ctggtcacgc acccataaca gtgcatcaat gactgtatta
    13201 ccggtaatga agattcggct atccgcaacg ttttcacgca gcaagttttg ccgggaagtt
    13261 tcggttggag agaagtgata catcgccaga tgcccggtca atgtacggtt agcctcttcc
    13321 ggccacggcg aatagagagc gcccgtgcgc agaccagcct caacgtgacc aacaggaata
    13381 cgctgataaa acgccgccag gctggttgcc agcgtcgtcg tcgtatcgcc gtgaaccagc
    13441 acgacgtctg gtttgaactc ggcaagaata ggttttagcc cttccagaat ccgacaggtt
    13501 atctctgtca ggccctgtcc tggctgcatt atgttgagat cgtagtcagg tacaatggaa
    13561 aagagtttca gtacctgatc aagcatctcc cgatgctgcg cagtgacgca aactttagcc
    13621 tcaaaaaaag gatcttttgc caacgcatgc accaacggag ccatcttgat ggcttccggg
    13681 cgcgtaccaa atacagtcag tactttcaca tcgattctct tcgaataagc ggcgagcgcc
    13741 tttgcgctca ccgcagcagt gttgctattt cgagcaacgg cgggttaatg cgacaccagc
    13801 cccgatcagc cccccgacaa tgccccacat aatcatcagg aaggcacgac gtgggctatc
    13861 gcgttttacc ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac gcggatccag
    13921 ggttggacca acattcaggg tgtttaacat ggctcgattc tgatcatagt cgagatcaaa
    13981 ggccggaccg acggcctgta aattttccag tcgagcctgg agcattggac gcccaagcag
    14041 gaacatttct gaatcaggta attcctcggc aggcacatct gtcgcactgc gcgaaatatt
    14101 atgctgctca gcaattttca gcgcctgctc aatactgttc atccggcggt cgtagatggc
    14161 tttcgccacc tcttcctgac gcttcacctg agctttcatc tggatggtgc gtgccgccca
    14221 tgcgcctttc agctcatcat tcagatggct ggctgcacgc tggctggcaa aagcaacata
    14281 ctgacgtaac aggttattag cgtcaggcgc ggtttcggca ataagcttca cgctgtcatt
    14341 gaccgcgcga gtaaagtctc cggggataaa ctggatgttg ttaatcattt catccagcaa
    14401 cgccgcatcg gctttgctgt tgcccaccat ccgctgtttg taatagtcgg tttgcagcca
    14461 gaactctctg cgggtatccc acgaggccag ttgcataaca aactctttat aggcttcgtc
    14521 catgaccgat ggttgatcgg cagaagccat gtttgaacgg acatccaggt tacgcaaaaa
    14581 ttgctgctgc gagtaatatc cccccagcat attcaccgtt ggacgatcgg taatcgccgt
    14641 cgagctccac tcctgacgag caaaaaaagt atacgccagc gcgattaacg caaacgccag
    14701 ccccatgcca ataatccata gcttcccagc ccacaaggta cgaaacaacc cacgaatatc
    14761 cagttcattt tcagcgtctt cggccggttt cccaggcatt ggttgtgtca tcacatcctc
    14821 atttatttgg ttaaattggg gctgccacca cgatttctac gcagtctgcg ttttacgcgc
    14881 ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat atccatagag gaagaatgct
    14941 agcaaaaaga gcaccagcat gacccactcc gggacaaaat gagaatattc tgccagcacg
    15001 ccaatggaag cgagcagtgc tgcggcaagg gtaatcagca caaacgcctg acgggaagta
    15061 aacccggcac gcatgatcaa atggtgaata tgctgacggt caggagagaa tgggctcatg
    15121 cctttacgca ggcgacggta cataatcgcc accatatcca ttagcggaat ggcgattatc
    15181 cacaaagcgg taaccgggct gatgggatgg gttttgccct gggtcgtttc gagcaggatc
    15241 cagataacgg taaaaccaat cagcgtactg cccgcatcac ccataaagac tttgtagcgg
    15301 cgacccagga taccaaggtt aagcatgatg tatggcagga tggcggcgat catcgcaaag
    15361 caccagattg cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac
    15421 gagacgcagg acaacccgcc cagcaagcca tcaatgccat caaccatgtt gaacgcatta
    15481 atggccgccc agacggcaaa tagcgtcagg aagtaaccaa acggtccgag caccatctcc
    15541 caggagccaa agatataacc caggctactg agataaagtt tgccgaacac catcataaca
    15601 atgccaacag cggcctgtat ggtggcacgg atttttacgc tgatatcaaa acgatcatcc
    15661 agcgcgccaa tgaaaacaag cacaccggca caagcgagat agagagatgc atgcggaata
    15721 tagtaatcga caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg
    15781 agaggtatca atccctggtg acgtttgcgg aagtttggtt tatccactaa accgactttt
    15841 tttgccacct tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat actgatgaga
    15901 tcagtactca ctgtcagtaa attcacaatg catgctctca gagaaaatta ttagcagaag
    15961 tataaccacg aagaccttta ttcagaaggg aaagtccgtt ccgaacccac cagccctgtg
    16021 caaaaatcaa ccatttgata tgcaaattgc ataacatgcc agcaaattcc aggaattttc
    16081 ctaacatcgc cagcgcgaca ttaagattac agatataaaa gcaaaacgcc acgtaaacac
    16141 gtggcgtttt tggcataaga caaatttatg agcgtttcat catttcgaag aaatcgtcat
    16201 tggtcttggt catggccagt ttattaatga ggaattccat tgcatcgatt tcgcccatcg
    16261 ggtgaatgat tttgcgcagg atccacattt tctgcagttc ttcctgagtc gtgagcagct
    16321 cttctttacg ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt ttttcagcga
    16381 tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg taaataactt
    16441 cgtccatttt agaaccggta tcgataagcg ccgtcgcgat aatggtcagg ctgccgccct
    16501 cttccacgtt acgcgccgca ccaaagaagc gtttcggacg atgaagggca ttggcgtcca
    16561 caccaccggt caacacttta cctgacgccg gaacaacagt gttgtaagcg cgcgccagac
    16621 gagtgatgga gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg cgtttggcct
    16681 tctcgatcac catttccgca acctgaacgt ggcgagatgc gggttcgtca aaggtagaag
    16741 caacaacttc accttttacc agacgctgca tctcggttac ttcttccgga cgttcgtcga
    16801 tcagcagaac catcagcaca caatccgggt ggttgtaagc aatgctctga gcaatgttct
    16861 gcagcagcat agttttaccg gctttcggcg gtgccacaat cagaccacgc tgaccacgac
    16921 cgataggtga tgccagatcc agtacgcgag cagttaaatc ttcagtagaa ccgttaccac
    16981 gttccatacg cagacgagag tttgcgtgca gcggggttaa gttctcaaag aggattttgt
    17041 tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc
    17101 gttcaccttc tttcggcggg cgaatcttac cagagatggt atcaccagtg cggaggttga
    17161 aacggcggat ttggctaggg gaaacgtaga tgtcatcagg accggcgagg taggagctgt
    17221 ctgcggaacg gaggaaacca aatccatcct gcaatatctc cagtacgcca tcaccaaaga
    17281 tatcttcgcc actctttgcg tgctgcttca ggatggcaaa aataatgtcc tgcttacgca
    17341 tgcgagccag gttttccagc cccatatttt cgccgagagt gatcagctca gaaaccggcg
    17401 tattctttaa ttcggtaaga ttcatagtgg tgtgagttct taaacttggg gtaaatctcg
    17461 aacttaatgt tgtgaatggt atggcaggat catccatgcc tgtttacggt catcgactca
    17521 tgtcttttcg ctgcctggtc acaggagagt acgcagaact gaaacgacaa gacggattga
    17581 gtgacgagcc agaaatctgt tcacttcgca tttaagataa aacgggaagc gttggaaatt
    17641 acaagattca aacttaataa ggtatgttta atacgaagtc aacactaagt tagcatgact
    17701 cacgccgggc gtccagtttt tagcgacggg gcacccgaac atgaaattcc cttacgccag
    17761 gttagcgtcg aggaactctt tcaactgacc tttagacagt gcgcccactt tggttgccgc
    17821 cacttcaccg tttttgaaca gcagcagagt cgggatacca cggatgccat atttcggcgc
    17881 agtgccaggg ttttgatcga tgttcagttt tgcaacggtc agtttgccct gatattcgtc
    17941 agcgatttca tccagaatcg gggcgatcat tttgcacgga ccgcaccact ctgcccagaa
    18001 atcgacgagg atcgccccgt ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag
    18061 gtgaataatt ttatcgctca tatataactc cacaggaata agcctggcgt gttggtttcg
    18121 ttgttggtgt aacattaacc aactaaaggt tgactttatt tcaccggata cgctttcgta
    18181 aagcaatagt aagctgatat tctaccacac tatgagcaaa acacatttaa cagaacagaa
    18241 gttttccgac ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca
    18301 taactgtacg cccattcagg cactggccct tccgctgacg ctggcgggtc gtgacgtagc
    18361 cgggcaggcg caaaccggta ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta
    18421 tcttctctct catcctgcga ttgccgatcg caaggtgaat caaccgcgtg ccttaattat
    18481 ggcaccgacg cgtgaacttg ccgtgcagat ccacgccgac gcggaaccgc tggcggaagc
    18541 taccggcctg aagctgggcc tggcttacgg tggtgatggc tatgacaaac agctgaaagt
    18601 gctggaaagc ggcgttgaca ttctgattgg caccacgggt cgtttaattg actacgccaa
    18661 gcagaaccac attaacctcg gtgccattca ggtagtggta ctggacgaag ccgatcgcat
    18721 gtacgatcta ggctttatta aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa
    18781 tcagcgcctc aacatgctgt tctccgccac gctttcgtac cgggtacgtg aactggcgtt
    18841 cgagcagatg aacaatgccg aatatattga agtggaaccg gaacagaaaa cgggccaccg
    18901 tattaaagaa gagcttttct acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct
    18961 gatcgaagaa gagtggccag acagagcgat tattttcgcc aataccaaac accgttgtga
    19021 agagatctgg ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt
    19081 cgcgcagaaa aaacgtctgc gtattcttga tgaatttacc cgtggcgatc tggatattct
    19141 ggttgccacc gacgttgccg cgcgtggttt gcatattccg gcagtgacgc acgtctttaa
    19201 ctacgattta cccgatgact gtgaagatta cgttcaccgt attggtcgta caggtcgcgc
    19261 aggcgcaagc ggtcactcta tcagcctggc gtgtgaagag tatgcattga atttgcctgc
    19321 tattgagacc tatattggtc actcaattcc ggtaagcaaa tacaatccgg acgcattgat
    19381 gaccgatctg ccaaaaccgc tgcgcctcac gcgcccgcgc acaggcaatg gtccgcgtcg
    19441 tactggcgct ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca
    19501 ttcaaactgt atcaagatgg caagagaatg aatcccgatg agcttactgt agtaagtgat
    19561 tcgggtgaaa gaatgcagcc aacacagaga cagattgaag gatgaagagt atgggttcca
    19621 cctcgtcgct gtatgcagcc attgatctcg gttcgaatag ttttcatatg ctggttgtgc
    19681 gcgaggtggc tggaagcatc cagacgctga cgcgaattaa acgcaaagtg cgtctggctg
    19741 ctggcctgaa tagcgaaaat gacctctcta atgaagcaat ggagcgcggt tggcaatgtc
    19801 tgcgcctgtt tgctgaacgt ctgcaagata tccctccctc gcaaattcgc gttgtcgcta
    19861 cggcgacatt acgacttgcc gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc
    19921 tcggttgtcc ggtacaggtg atcagcggtg aagaggaagc gcgtctgatt tatcagggcg
    19981 ttgctcacac cactggtggt gccgatcagc gcctggtggt ggatataggc ggtgccagta
    20041 ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct
    20101 gcgtcacctg gctggaacgc tattttgccg atcgtaatct ggggcaagaa aattttgatg
    20161 ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc cgatgaatta cggtatcacg
    20221 gctggaaagt gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa atcatgatgg
    20281 cacaggggat ggatgaacgc attaccctgg aaaagttgca gcaattgaaa cagcgagcca
    20341 ttcattgcgg tcggctggag gaactggaga ttgacgggct gacgctggaa cgtgcattag
    20401 tgttcccgag tggtctggcg atcctgatcg ccatttttac cgaactgaat attcagtgta
    20461 tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg catctggccg
    20521 tcgagcagga tattcgcagc cgtacactgc gtaatattca acgccgcttt atgatcgata
    20581 ttgatcaggc acagcgcgta gccaaagttg cggctaactt cttcgatcag gtggaaaacg
    20641 aatggcatct tgaagcaata agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa
    20701 tcggcctgag cgttgacttc aaacaagctc cgcaacacgc tgcttatctg gtgcgtaacc
    20761 tggatcttcc cggttttacc cccgcacaga agaaactgct ggcgacgcta ctgctcaacc
    20821 agactaaccc ggtcgatctc tcatcgctgc atcagcaaaa tgccgtacca ccgcgcgtcg
    20881 cagaacaact ctgccgttta ctccgcctgg ctatcatttt tgccagccgt cgccgtgacg
    20941 atctcgtgcc agagatgaca ttacaggcta accatgaact gttgaccttg acgcttccgc
    21001 aaggttggct aacccaacat ccgctgggta aagagattat tgctcaggaa aaccagtggc
    21061 agagctatgt ccactggccg ctggaagtgc attaatgagt aagtgccgga tgcgatgctg
    21121 acgcatcttc tccggccttg attatttccc tcgttttgcc gccatcatcg ctttcagatt
    21181 cgccagatgg ctttgccctt tctgcatccg ttcttcggca ctgaccactt tgcgctcctg
    21241 ttcccaaatc agatcatcct gcggcagctc cagcaaaaag cggctcggct ccgggcgcac
    21301 cagttcgccg tactgacggc gttctttaca cagcgtaaag gtcaattcct tctgggcgcg
    21361 ggtaatgccg acataggcca gccgccgctc ctcatcgata ttatcttcat cgatactgct
    21421 ctggtgcggc aaaaaccctt cttccatacc gaccatgtag acataaggaa actccagccc
    21481 ttttgacgcg tggagagtca tcagttgcac ctgatccagc tcttcttcac tctcaccacg
    21541 ctccatcatg tcgcgtaaag taaagcgcgt caccacctgg gtgagcgtca tcggctcatc
    21601 cagttcgctg ccttccagca tctccgtcat ccagctaaac agttggttga cgttcttcat
    21661 gcgcatttcg gcagctttcg tgctgggcga tgtttcgtac agccaggatt cataatccat
    21721 gccgtggatc agatcgcgca ccgcggcaat cggctcccgc tccgccagac gctggatttc
    21781 tgccaaccag tgagtgaagc gggtcaatgc ttcataacca cgtccgctaa gcgtctgact
    21841 caggcccata tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg cccactcacc
    21901 cagctttttc agcgtcgccg ggccaatctc tcgcttcggc gtgttaacga tacgcagaaa
    21961 tgcgctgtca tcgtccgggt tagtcagcac gcgcagataa gccagcaagt ccttgatttc
    22021 aggacgagag aaaaacgacg taccaccaga aattttgtac gggatgcggt tttgcatcag
    22081 gaatttttca aacacccgcg actgatagtt accgcgataa agaatagcgt aatctttgta
    22141 ctgcgtttta ttgacgaagt gatgcgcaat cagttcacca gtgacgcgct cggcttcatg
    22201 ttcttcgtta ttcgcgctta aaacttttag ctctgtgccg taacccagtt cggagaacag
    22261 acgcttttca aagacgtgcg ggttattggc gatcaggatg ttcgccgctt tcaaaatacg
    22321 tccggaagag cggtagttct gctcaagctt aatcaccttc agcgccggaa aatcctggct
    22381 cagcagcacc aggttttgcg gacgtgcgcc gcgccaggag tagatcgact ggtcatcgtc
    22441 acccaccacg gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt catactggct
    22501 ggtgttggtg tcctgatatt cgtccaccag caggtagcga attttgttct gccagcgctc
    22561 gcggacttct tcattgcgtt gcagaagtag cgtcggcaat aaaatcagat catcgaagtc
    22621 gagcacatta caggctttca ggtgtgcatc atacagccca taacaatggg caaaaatacg
    22681 gtcccgctcg ccaatcgcac tggctgccgc ctgggacggt gttttgagat cgttcttcca
    22741 gttagagatg gtcgaaatca actgttgcag gagaactttg tcatcttcaa tcagcccttc
    22801 ggtcaactct ttgagcaacg ccagctgatc ggtatcgtca aacagcgaaa agttcgcctt
    22861 catccccagc gccgcatact cacgcttgat gatatccagc cccagcgtgt ggaaggttga
    22921 gatcatcagc ccatgcgcct ctttgcgacc cagcgtctgt ccgacacgct ctttcatctc
    22981 gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaccc
    23041 gcggatcaga tgggcgattt tattggtgat aacacgagtt ttaccggaac ccgcacccgc
    23101 cagcaccagg caggggccgg taacgaattc gacagcttgt tgttggccgg ggtttagacg
    23161 cataggaaaa tcgctcaatg aaagtcggga ggggaaaata acagcgtggt agtatagcca
    23221 gcctcatcct taccactcaa ggcacgatca tggcaaaaac agcagcagca ctgcatatcc
    23281 ttgtaaaaga agagaaactg gctctggatc ttctcgtgca gattaagaac ggtgccgatt
    23341 tcggcaagct ggcgaagaaa cactccattt gcccatcagg caaacgcggc ggtgatttag
    23401 gtgaattccg ccagggtcag atggttccgg cgttcgataa agttgtgttc tcttgtccgg
    23461 tactggagcc aaccggcccg ctgcacaccc agttcggtta ccacatcatt aaggtgctgt
    23521 accgtaacta atagcaaggc cttctccggg agaaggcctt gaactctgaa tcgctattaa
    23581 cccgcaacag caatacgttt catatccgtc atatagccgc gcagtttctt acctacttgc
    23641 tcaatcgcat ggctgcgaat cgcttcattt acatcacgca gctgcgcgtt atctaccgca
    23701 ccttccggaa tagctttacc caaatcgccc ggttgcagtt ctgccataaa cggtttcagc
    23761 agcggcacac aagcgtaaga gaacagatag ttaccgtact cggcagtatc agagataacc
    23821 acgttcattt catacagacg cttacggacg atggtgttgg caatcagcgg cagttcgtgc
    23881 agtgattcat agtaagcaga ttcttcgatg atgccggaat caaccatggt ttcgaacgcc
    23941 agttcaacgc ccgctttcac catcgcgatc atcagtacgc ctttatcgaa gtactcctgc
    24001 tcgccgattt tgccttcata ctgcggcgcg gtttcgaagg cggttttgcc ggtctcttca
    24061 cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc ggaggagaat
    24121 tcgccggaga tgatgtcgtc catatgtttc tggaacagcg gtgccatgat ctctttcagc
    24181 tgttcagaca acgcgtaagc acgcagtttc gccgggttag agagacggtc catcatcagt
    24241 gtgataccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg aattagtttt
    24301 tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag cagcgagcca
    24361 gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt cacttccgca
    24421 acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc ccatgcttta
    24481 gcgatcgcca tgccttcgcc tttcggatcg ttttccgggt gaacggcaat cagcgtcggt
    24541 acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt cggcgcaacc
    24601 atgactacgg tgatgtcttt acggatctgc tcgcccactt cgacgatgtt gaaaccgtgc
    24661 gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac tacatcagag
    24721 tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gtgggatcag ttcttcgtaa
    24781 gtacccactt taaaaccatt ttcggtcgct ttacgccagg acgcgcgctt ctcggcaatc
    24841 gcttctttac gcagagcgta ggagatatcg agaccagaat cacgcatgtt cagaccctgg
    24901 ttcagacctt gtgcgccaca gccgacgatg actacttttt taccctgaag gtagctcgcg
    24961 ccatcggcga attcatcgcg ccccataaag cgacatttgc ccagctgtgc cagctgctga
    25021 cgcagattca gtgtattgaa gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt
    25081 attgttcggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt tattgcggaa
    25141 attgatatat tcacaacgtc acattgcaat ttctgcaacg tcaacattga gggctgcgcc
    25201 attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc attttggccg
    25261 cagcgcgcgg gcgatgcacg ttagcccgtc cacgctctca cggcagattc agcgcctgga
    25321 agaagatctc ggccagccgc tgtttgtgcg cgataaccgc acggtgacgc tgactgaagc
    25381 gggcgaagaa ctgcgcgttt tcgcccagca aacgctgttg cagtatcagc agctgcgcca
    25441 caccatcgac cagcaagggc cgttgctcta tggcgaatta catatcttct gctcggtgac
    25501 cgccgcttac agccatctgc cgccgattct ggatcgcttc cgcgcggaac acccgtcggt
    25561 ggagatcaaa ctcactaccg gtgatgcggc agatgcaatg gaaaaagtcg tcacaggtga
    25621 agctgatctg gcgattgccg ggaagccaga gactctgccc ggcgcggtgg cgttttcgat
    25681 gttggagaat ctggcagtgg tgttgattgc acccgcattg ccctgcccgg tgcgtaatca
    25741 ggtttctgtc gaaaagccgg actggtcaac ggtaccgttt atcatggcca atcaggggcc
    25801 ggtgcgccgc cgcattgagt tgtggttccg acgcaataaa atcagtaacc cgatgattta
    25861 cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct gtggcgtggc
    25921 attgttgccg gaagtggtgc tggaaaacag ccccgaaccg gtgcgtaacc gcgtgatgat
    25981 tttagagcgc agcgatgaga aaacgccgtt tgagctgggc gtgtgtgcgc agaaaaagcg
    26041 gctacatgag ccgctaattg aggcattctg gaagattttg ccgaaccatt aacccgccaa
    26101 aaagaacctg aacgccgggt tattggtttc gtcgtggcaa tcgtagccca gctcattcag
    26161 ccgggtttcg aaatccggtt catggtcgcc aagttcgaac gccgccagta cgcgcccgta
    26221 gtcggtgcca tggctgcgat agtggaacaa agaaatgttc cagtgcgtac ccagcgtgtt
    26281 gaggaagcgc agcagcgcgc ccggtgattc cgggaattcg aagctgtaga ggcgttcctg
    26341 caacggatgt gacggacgcc cgccgaccat atagcgcaca tgcaacttcg ccatttcgtc
    26401 gtcggagaga tccaccacgc tgtagccgcc gtcgttgagc atctgcaaaa tttctttgcg
    26461 ctcttcgagg ccgcggctca ggcgcacgcc gacaaagatg caggcgtttt tggcatcggc
    26521 aaaacggtag ttgaactcgg tgaccgatcg cccgccaagc agttggcaga atttgaggaa
    26581 gctgcctttt tcttccggaa tggtcaccgc caacaacgct tcacgctgtt cgcccagttc
    26641 gcagcgttcc gagacgtagc gcaggccgtg gaagttcacg ttggcaccgg aaagaatatg
    26701 cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat tttttcattc ccgccagcgc
    26761 cagcgcgcca gagggttccg ccaccgcacg cacatcttcg aataaatcct tcatcgccgc
    26821 acagatcgca tcgctatcga cggtgatgat gtcgtcgaga tactcctggc ataaacggaa
    26881 ggtttcgtca ccgatgcgtt ttaccgcgac accttcggcg aacagtccca cgcgtggcag
    26941 atcaaccgga tgacccgcat ccagcgctgc tttcaggcag gcggagtctt ccgcttctac
    27001 ggcgatcact ttgatttgcg gcatcagttg tttgatcagc accgctacgc ccgccgccag
    27061 accgccgccg ccgactggca caaatacgcg gtcgagatgg gcgtcctgct ggagcagttc
    27121 cagcgccagc gtgccttgcc cggcaatcac catcggatgg tcgaacggcg gcacccaggt
    27181 gaacccctgc tgctgtgaca gttcgatcgc tttagctttc gcttcgtcaa agttcgcgcc
    27241 atgaagcagc acttcgccgc cgaatccacg taccgcatcg actttgatgt cggcggtggc
    27301 ggttggcata acgatcaggg ccttcacgcc taaccgcgca gaagaaaacg cgacgccctg
    27361 cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct ttttgttctt ccgtcaggcc
    27421 cgccatcatg gcgtatgcgc cgcgcagctt aaagctgtgc actggctggc gatcttcgcg
    27481 cttcaccaga atgacgttat caagacgcga cgacagtttt tccatttttt gtagcggcgt
    27541 aacctgcgcc gcctcgtaaa ccggcgcgcg cagcactgct cttaaatatt cggcaccttc
    27601 cggagcaccg gacaggggtt gcgagtcagc cattattaac cccccagttt cgatttatcg
    27661 cgcaccgcac ctttgtcggc gctggttgcc aggctggcgt aggcgcacag ggcaaaggaa
    27721 acctgacgtt cacggttttt cggcgtccag gctttgtcac cccgagcttc ctgcgcttca
    27781 cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa tgccacggtt cgggatgtcg
    27841 atagcgatca ggtcaccatc ttcaatcaga ccaatgctgc cgccgcttgc cgcttccggt
    27901 gagacgtggc cgatggaaag accagaggtg ccgccagaga aacgaccgtc ggtgatcagc
    27961 gcacaggctt taccgagacc cattgatttc aggaagctgg ttgggtagag catttcctgc
    28021 atgccagggc cgcctttcgg gccttcatag cggatcacca ccacatcgcc cgcgacaact
    28081 ttgccaccga ggatcgcttc taccgcgtcg tcctggcttt cgtacacttt cgccgggccg
    28141 gtgaatttga ggatgctgtc atcgacgccc gcggttttca cgatgcagcc gttttcagca
    28201 aaattaccgt agagcaccgc caggccgccg tctttgctgt aggcgtgttc cagcgagcgg
    28261 atacagccgt ttgcgcgatc gtcatcgaga gaatcccaac ggcaatcctg cgagaatgcc
    28321 tgtgtagtac gaatgcccgc agggcctgcg cggaacatat tttttaccgc gtcatcctgg
    28381 gtcagcataa cgtcgtattg ttccagagtt tgcggcaacg tcaggccaag tacgtttttc
    28441 acatcacggt tcagtaaccc cgcgcgatcc agttcaccga gaataccgat aacaccacca
    28501 gcacggtgaa catcttccat atggtatttt tgggtgctcg gcgcaacttt gcataactgc
    28561 gggactttac gcgaaagttt gtcgatatca ctcatggtga agtcgatttc cgcttcctgc
    28621 gccgccgcca gcaggtgaag taccgtgttg gtagagccgc ccatcgcgat atccagcgtc
    28681 atggcatttt caaacgccgc tttgctagca atattgcgcg gcagtgcact ttcgtcgttt
    28741 tgctcgtagt aacgtttggt caattcaaca atgcgtttac cggcattgag gaacaactgc
    28801 ttacggtccg cgtgggttgc cagcagcgag ccgttgcccg gctgcgacag gcccagcgct
    28861 tcggtcaggc agttcattga gttagcggta aacatcccgg agcaggagcc gcaggtcgga
    28921 cacgcggaac gttcaacctg atcgctctgg gagtcagaaa ctttcgggtc tgcgccctgg
    28981 atcatcgcat caaccagatc gagtttgatt atctgatcgg acagtttggt tttcccggcc
    29041 tccatcgggc cgccggaaac aaagatcacc ggaatattca ggcgcaggga agccatcagc
    29101 atccccgggg tgattttgtc gcagttagag atgcagacca tggcgtcggc gcagtgggcg
    29161 ttgaccatat actcaacgga atcagcgatc agttcgcgag atggaagtga ataaagcatc
    29221 cccccgtggc ccatggcaat cccatcatcc accgcaatgg tgttgaactc tttggcaacg
    29281 ccgccagccg cttcaatttg ttcggcgacc agtttaccga gatcgcgcag atggacgtga
    29341 cccggtacaa attgggtgaa cgagttcaca accgcgataa tcggcttgcc aaaatcggcg
    29401 tcggtcattc cggtggcgcg ccacagcgca cgagcacccg ccatattacg accatgagtg
    29461 gtggtggcgg aacggtactt aggcatactt tatttactcc cagtgtctgt ctcgtaaatg
    29521 ggacggtgcg tgccgtccca ttttttgtat ttattgatta acttgatcta accagcccca
    29581 tttatcttcg gtttcgccag tgaagaggcc gaagaaggct tgctgaatgc gtttggtaac
    29641 cgggccacaa cggccttcgc caatctgaat accgtctacg ctgcgcactg gcgtgatttc
    29701 tgccgccgta ccggacataa agacttcatc cgccaagtac agggattcgc gcgacagcac
    29761 ctgctcacgc acttcaattc ccagctcttt cgccagtttg atgatggcat cacgggtaat
    29821 acccggcagc gcggaggagg tgaacggtgg ggtgaacagc acaccatctt tcacttcaaa
    29881 caggttttcg cctgcgcctt cagagatata accgttcaca tccagcgcga taccttcctg
    29941 ataaccgtgg cggcgcgctt cgctacccac cagcagggaa gagagatagt taccaccggc
    30001 ttttgccgca gttgggatgg tgtttggtgc tgcgcggttc caggaggaaa ccatcgcatc
    30061 gatcccctgc tccagcgctt ctgcgcccag atacgctccc cacgggaaag cggcgataat
    30121 cacatcggtt gagtatcccg ctggcgggtt tactcccatg ccaacatcac cgacgaagat
    30181 cagcggacgg atataggcgc tggtgagatt gtttttgcgg atcacgtcac gacaagcttc
    30241 catcagctca tcaatgctct gtgaaaccgg gaagcgatag attttggcgg agtcatgcag
    30301 acgctgcata tgctcacgat ggcggaatac aaccggtcct ttgtgtgagt cgtagcaacg
    30361 gatgccttca aaaaccgagg tgccatagtg cagcgcgtgc gacatcacat gcaccttcgc
    30421 gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa tcagctttct tcgtggtcat
    30481 ttttatattc cttttgcgct caggcgcgga tttgttgtga tgtggttgtg ctctggcaga
    30541 tggcaacgtg tgcgacgtcc accagtttat ttaactgact aaacagtaag tcgaccgacc
    30601 gtgggctggc aacggtcaat tcgatattta tattttgtgc atcgctggcg gcagccatat
    30661 tcattgagca gacgtggaaa ccacgatgac gcaccacgcg taaaacacgt tctaaggttt
    30721 ccggattgaa gcgagccgat acattgacct gatgttgcat catgataatt tctccaacat
    30781 ttctgaatta ctggcacctg gcggcaccag cggccagacg ttctcaagtt cgtcgattga
    30841 gacatgaagc aggtatggcc catcactgtt cagcatggtg tcgagtgccg cttcaacctg
    30901 gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg ctggctaaca tgaggaaatc
    30961 ggggttatcg gtaagggtgg tttcgctgta tcgttcctga aaaaacagtt gctgccattg
    31021 tcgaaccatc cctaaccgtt ggttatcgag taagacgatt ttcaacggta actgcttgcg
    31081 ttttacggtg cccagctctt gcacattcat catgaaagag ccgtcaccgg agatacagac
    31141 aacggtatcg ttcggtcgcg cgacttgtgc gccaactgcc gccggtaaac caaaacccat
    31201 ggtacctaag ccgctggagg tgatgaaatt ttccgggcga gtgtgggcga tgtgctgcgc
    31261 agcccacatc tggtgctgcc ccacatctgt ggtcacgacg caatccgcag gtttacgatc
    31321 cgacagttgt tttaacaaca acggagcgta gatagcgtca ccgggatggt cgtaacgcca
    31381 ggaatgttca tcacgcagct gcgcgcagtg ttgctgccag tcattgatat ttaacggctg
    31441 ctgtaatgct ggtaacagag catttaaatc accttgtaat gccacatgtg cctgacgcag
    31501 cttgttcatt tctgccgggt cgatatccat atggataaca ctggcgtgtg gtgcgaaggt
    31561 gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc acggcgatca gcaggtcaca
    31621 ctcctgcacc gcgaagtttg ccgctttggt tccgtgcatc cccagcatgc ccagatagta
    31681 cggataatct gcttctactg cgcccagccc tttcagcgta caggtggcag gcatttttgt
    31741 ggcagcgaga aattcacgca aagccggaac tgcctgcgcc atccccacgc caccgccaac
    31801 gtacagcatc ggtttttgcg cttttgccag catctggcgc gcttgctcga cttcggcatg
    31861 tgggaaagtc acttcgtttt caacggtggt gaaccacggt tccagatcgc cgctggctaa
    31921 ctggatatct tttgggatat cgaccagaac cggaccagga cgacctgagc tggcaacgtc
    31981 gaatgcttca gccatgatgc gcggcaactc ttccagagac tgcaccagaa agctgtgctt
    32041 ggtacaggct aacgacaatc ccaggacatc cacttcctga aatgcgtcgg tgccgataaa
    32101 cggtgcggac acctgaccag tgatggcaac gacagggatg gaatctaaca gtgcgtccgc
    32161 aagcccggtt atcaggttgg ttgcgcccgg accagacgtg gcgatacata cgccagtttt
    32221 accggtagca cgggcataac cgatagccgc cattgccgca ccctgctcat gtcggcacag
    32281 caagtgctcc acgccgccgt catacaatgc atcgtaaacc ggcataattg cgccacccgg
    32341 ataaccgaaa acggtgttca caccctgtgc ccgcaacgca tgtaccaccc actgtgcgcc
    32401 attcatagtt agttccccgt cctgaatctt gagaaacaga attttgtgct gttattcatt
    32461 gtctgctcct cggttatgtt tttaaggtca aaaaaacccc cggacctttc ggtgcggggg
    32521 tcttagttcg ttaaggcttg atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg
    32581 tgggataata atcaccacca cgctaatcac gaccaggcta atcactcgta gaagggctgt
    32641 cattttgtct tttcttgcat cttgttcgaa ggaatgccta aagagttacc atagattttg
    32701 cgaatagcac aagatatttt tgggcctatt tttatgatgg ctttaataat ttcaatagaa
    32761 attgttgttt tttaaaaaga aaatagtgag tgaaaaaatt caatttcagt ataaatgttg
    32821 ttcccttcct cgtagttcat cctttgtccg ctgcattttt gcgagcatca ttccaccgcc
    32881 agaacaactc aacttcactt gcacaaataa acaggaatca gccactaaaa ttcacgaatt
    32941 agcgggctac agcagcgata tcttatgcac ataatggtga ctcaaggagg gtttatgtca
    33001 ctgtcaattg ttcatacccg cgcagccctg gaagtaaatg cgcccccaat cactgttgag
    33061 gtacatatca gtaaaggtct acccggctta acgatggtgg gcttaacaga aacaacggtg
    33121 aaagaagctc gcgatcgcgt gcgcagcgcc attatcaata gcggatatga atatccggcg
    33181 aaaaaaatca ccatcaacct tgctccagcc gatctgccaa aagaaggggg acgatatgat
    33241 ttacctatcg ccattgcgtt gctggcggcc tcagaacagc ttacagccaa taagttagat
    33301 gaatatgaat tagtcggaga actagcgctt acaggcgctc tgcgtggcgt tcccggtgca
    33361 atctccagtg caactgaagc tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac
    33421 gaagatgaag tggggctaat taacggtgaa ggatgcctga tagccgatca tctgcaggct
    33481 gtctgtgcgt ttctggaagg taagaacact ctcgaacgcc cgaaaccaac tgatgcagta
    33541 tcccgggcgc tacaacatga tctcagtgat gttgtcggtc aggagcaagg aaaacgagga
    33601 ctggaaatta ccgccgctgg tgggcacaac cttttactga ttgggccgcc gggaacaggt
    33661 aaaacaatgc tcgccagccg tattaatggc ctgttgccag atttaagcaa tgaagaggca
    33721 ctggagagcg ctgcgatatt aagtctggta aatgctgaat cagtacaaaa acaatggcgg
    33781 cagcgcccgt tccgctcacc tcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt
    33841 gcaattccag ggccaggtga aatttcgttg gcgcataacg gcgtgctttt tcttgatgag
    33901 ctacctgaat ttgaacggcg tacactggat gccttgcgag agccgattga atccgggcag
    33961 atccatcttt cacgcacacg agcaaaaata acctatccag cccgtttcca gcttgttgcg
    34021 gcgatgaatc ccagccctac cggacattat cagggaaacc ataaccgctg cacgccagaa
    34081 cagacattac gttatctcac tggagatccc attaccaccc cccggcattt tgagtaaaac
    34141 ggtagtgccg ggagaaagca gcgccaccgt taaacaacgc gtaatggccg ccagagagcg
    34201 ccaatttaag cggcagaata aactgaatgc ctggctggat agtccggaaa tacgccaatt
    34261 ctgcaaactt gagagcgaag atgcgatgtg gttggaagaa acgctgatcc atctggggtt
    34321 atcgattcgt gcctggcagc ggttactgaa agttgcacga accattgctg atattgatca
    34381 gtctgacatt atcacacgtc agcatttgca ggaggcagtt agctatcgtg cgattgaccg
    34441 tttgctcatc catctgcaaa aactactgac ataaaaaaag ggcatttcgc cctttttatt
    34501 aatcgtcaga atcggtgtag tcttcagcac cttcaacctg cggtttaccg ccggaaaggg
    34561 tgtgaaaacg ttttggacgc ttaatacgcg tcatatactt ggaccacacg cgttctgctt
    34621 ctgtcactgg ctcacgttcg ccacggcata ctgctacaaa gagtttctct tcctcggtaa
    34681 ccggctcgcg tttgccaaga tccaactcat tgaaggcata accatgacgc tcaagcagtt
    34741 gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc acgtggataa tgtttattgt
    34801 cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct gcacgctcct aattctttga
    34861 ccgagctagt tatggcgcgg agtattagtt acgcttgaca gagtgtaaaa caaaacattt
    34921 aaatcataac gacaaataat tttgcggaga gcactgtgga tacggaattg ttaaaaactt
    34981 tcctggaagt tagccgaacg cgtcactttg gtcgagcggc tgaatcgctc tatctgaccc
    35041 agtcagcagt gagctttcga atcagacaac tggaaaatca actgggtgtg aaccttttca
    35101 cccgccacag aaacaatatc cgtttaaccg ctgccggtga aaaactactg ccttatgcag
    35161 aaacgctcat gagcacctgg caggccgccc gcaaggaggt ggcgcatacc tcacgacata
    35221 acgagttttc tatcggtgcc agcgcctcgt tgtgggaatg tatgcttaat cagtggttgg
    35281 gacgcttgta tcaaaatcag gatgcccata caggcttaca gttcgaagcg cgaattgccc
    35341 aacggcagtc tctggtaaaa cagctgcatg aacgccagct tgatcttctt attaccactg
    35401 aagcgcccaa aatggacgaa tttagtagtc agttgctggg gtatttcact ttagcgcttt
    35461 ataccagtgc cccttctaaa ctaaagggag atcttaacta tctgcgactg gagtgggggc
    35521 cagattttca acagcatgag gcaggtttga tcggtgctga cgaagttccc attctgacaa
    35581 ccagttctgc tgaactggca cagcaacaga ttgcgatgct taatggttgc acctggctac
    35641 ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt tgtcgatagc acaacacttt
    35701 cacggccgct ttatgccata tggctgcaaa atagcgataa aaatgcgttg attcgcgatc
    35761 ttttgaaaat taacgtgctg gatgaagtgt attaatctga atggctggca aggatgccgg
    35821 tagaaggatt tacttcggag aggattattt cagataaaaa aaatccttag ctttcgctaa
    35881 ggatgatttc tggcaggggc ggagagactc gaactcccaa cacccggttt tggagaccgg
    35941 tgctctacca attgaactac gcccctaatt agggtggcgg aacggacggg actcgaaccc
    36001 gcgaccccct gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc
    36061 ttttacaacc
//
LOCUS       DAJTPG010000031        35609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTPG010000031 DAJTPG010000000
VERSION     DAJTPG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(152..910)
                     /gene="deoR"
                     /locus_tag="OO230_001635"
     CDS             complement(152..910)
                     /gene="deoR"
                     /locus_tag="OO230_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5691436.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(968..1564)
                     /gene="ybjG"
                     /locus_tag="OO230_001636"
     CDS             complement(968..1564)
                     /gene="ybjG"
                     /locus_tag="OO230_001636"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5691437.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1849..3070
                     /gene="mdfA"
                     /locus_tag="OO230_001637"
                     /pseudo
     CDS             1849..3070
                     /gene="mdfA"
                     /locus_tag="OO230_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(3111..3395)
                     /locus_tag="OO230_001638"
     CDS             complement(3111..3395)
                     /locus_tag="OO230_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691438.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3481..4296)
                     /gene="ybjI"
                     /locus_tag="OO230_001639"
     CDS             complement(3481..4296)
                     /gene="ybjI"
                     /locus_tag="OO230_001639"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5691439.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4296..5504)
                     /locus_tag="OO230_001640"
     CDS             complement(4296..5504)
                     /locus_tag="OO230_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5691440.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5588..6124
                     /gene="rcdA"
                     /locus_tag="OO230_001641"
     CDS             5588..6124
                     /gene="rcdA"
                     /locus_tag="OO230_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5691441.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6299..7984)
                     /locus_tag="OO230_001642"
     CDS             complement(6299..7984)
                     /locus_tag="OO230_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5691442.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8254..8631
                     /locus_tag="OO230_001643"
     CDS             8254..8631
                     /locus_tag="OO230_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5691443.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8661..8918)
                     /gene="grxA"
                     /locus_tag="OO230_001644"
     CDS             complement(8661..8918)
                     /gene="grxA"
                     /locus_tag="OO230_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5691444.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9078..9365
                     /locus_tag="OO230_001645"
     CDS             9078..9365
                     /locus_tag="OO230_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5691445.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9349..10071
                     /gene="nfsA"
                     /locus_tag="OO230_001646"
     CDS             9349..10071
                     /gene="nfsA"
                     /locus_tag="OO230_001646"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5691446.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10132..11034
                     /gene="rimK"
                     /locus_tag="OO230_001647"
     CDS             10132..11034
                     /gene="rimK"
                     /locus_tag="OO230_001647"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5691447.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11122..11598
                     /locus_tag="OO230_001648"
     CDS             11122..11598
                     /locus_tag="OO230_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5691448.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11950..13062
                     /gene="potF"
                     /locus_tag="OO230_001649"
     CDS             11950..13062
                     /gene="potF"
                     /locus_tag="OO230_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5691449.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13157..14290
                     /gene="potG"
                     /locus_tag="OO230_001650"
     CDS             13157..14290
                     /gene="potG"
                     /locus_tag="OO230_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5691450.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14300..15253
                     /gene="potH"
                     /locus_tag="OO230_001651"
     CDS             14300..15253
                     /gene="potH"
                     /locus_tag="OO230_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5691451.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15250..16095
                     /gene="potI"
                     /locus_tag="OO230_001652"
     CDS             15250..16095
                     /gene="potI"
                     /locus_tag="OO230_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5691452.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16155..16643
                     /locus_tag="OO230_001653"
     CDS             16155..16643
                     /locus_tag="OO230_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5691453.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16684..17811
                     /gene="rlmC"
                     /locus_tag="OO230_001654"
     CDS             16684..17811
                     /gene="rlmC"
                     /locus_tag="OO230_001654"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5691454.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17986..18717)
                     /gene="artJ"
                     /locus_tag="OO230_001655"
     CDS             complement(17986..18717)
                     /gene="artJ"
                     /locus_tag="OO230_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5691455.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19009..19677)
                     /gene="artM"
                     /locus_tag="OO230_001656"
     CDS             complement(19009..19677)
                     /gene="artM"
                     /locus_tag="OO230_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5691456.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19677..20393)
                     /gene="artQ"
                     /locus_tag="OO230_001657"
     CDS             complement(19677..20393)
                     /gene="artQ"
                     /locus_tag="OO230_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5691457.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20400..21131)
                     /gene="artJ"
                     /locus_tag="OO230_001658"
     CDS             complement(20400..21131)
                     /gene="artJ"
                     /locus_tag="OO230_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5691458.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21149..21877)
                     /gene="artP"
                     /locus_tag="OO230_001659"
     CDS             complement(21149..21877)
                     /gene="artP"
                     /locus_tag="OO230_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5691459.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22095..22610)
                     /locus_tag="OO230_001660"
     CDS             complement(22095..22610)
                     /locus_tag="OO230_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5691460.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23259..25028
                     /locus_tag="OO230_001661"
     CDS             23259..25028
                     /locus_tag="OO230_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5691461.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25239..25562
                     /locus_tag="OO230_001662"
     CDS             25239..25562
                     /locus_tag="OO230_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5691462.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25559..26389
                     /gene="amiD"
                     /locus_tag="OO230_001663"
     CDS             25559..26389
                     /gene="amiD"
                     /locus_tag="OO230_001663"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5691463.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26386..27399)
                     /locus_tag="OO230_001664"
     CDS             complement(26386..27399)
                     /locus_tag="OO230_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5691464.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27498..28928)
                     /locus_tag="OO230_001665"
     CDS             complement(27498..28928)
                     /locus_tag="OO230_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5691465.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28939..29940)
                     /gene="ltaE"
                     /locus_tag="OO230_001666"
     CDS             complement(28939..29940)
                     /gene="ltaE"
                     /locus_tag="OO230_001666"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5691466.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29977..31695)
                     /gene="poxB"
                     /locus_tag="OO230_001667"
     CDS             complement(29977..31695)
                     /gene="poxB"
                     /locus_tag="OO230_001667"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5691467.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31828..32796)
                     /gene="hcr"
                     /locus_tag="OO230_001668"
     CDS             complement(31828..32796)
                     /gene="hcr"
                     /locus_tag="OO230_001668"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5691468.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32808..34460)
                     /gene="hcp"
                     /locus_tag="OO230_001669"
                     /gene_synonym="priS"
     CDS             complement(32808..34460)
                     /gene="hcp"
                     /locus_tag="OO230_001669"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5691469.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34604..35503)
                     /gene="lysO"
                     /locus_tag="OO230_001670"
     CDS             complement(34604..35503)
                     /gene="lysO"
                     /locus_tag="OO230_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5691470.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8870 a         8803 c         9148 g         8788 t
ORIGIN      
        1 ccgctgatcg tgatggaaaa tgtggaagag ggcggattct ttggtaggat gtgggatttc
       61 gtgatgatga aattccatca gtggttcggc agctggttct cttaatcttc tgataaccgg
      121 atggcgcgaa acgtcatccg gttatacgtc attaatacat caacttaata cggtgcgtct
      181 gcgcgtactt cacatactca tcttccgggc aacaatcact caccacaata tcaaagcgtt
      241 tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt actgtggtcg acaaccagca
      301 catgcttttg cgccatcgac atggcccagt gttttaccgg caactcttca agattaaaac
      361 aggtagcgcc tttactgaca tgcacgcccg ccgcggaata aaaagcgata tccgggcaaa
      421 aattattcaa cgtttgctga aaatcgatgg gtttgaaaat ggcgttgctg gcgtgaaatt
      481 caccaccgca cagaaatgcg cggcaatggg gtttctcttt cagcgccaga aaggtattta
      541 gcgaataaca aacggcggta aaagggattt cattatcaat cgcttcaata atccacggcg
      601 tggtggtgcc acagtcaaaa aagagggtct gatcgggttc taccagtgtc gcagccagtt
      661 ttgccgcccg gcgtttttct tccaccaggc gggatttttg atcgcttaac aggtaatggc
      721 tggcactgcg cggttccaga acaatatagc cgccgagcaa aacgacgggc gcactgtggt
      781 tgttcagatc gcgacgaatc gtcatctccg aaaccccaag cagggcggcg gcgtctttaa
      841 gatgtaactt atcgctgcgt tttaattctt gcagcagctg cccgatacgc tcttcgcgac
      901 gtgtttccat aatccctctg aatagttatt gaagcgagcc gctcaatact acacttttta
      961 gcagagatca gtcacgcacc cagcctttgc ggatcggtaa tgcaaaacag acgcgatacc
     1021 acgattgcag acgttgatag agtttatgcc ccatcgcttg ccagataatc tgggcactaa
     1081 ggcagccaat catacctgcc agcaatccac cgagcatatc cagcggccag tggacgccaa
     1141 gataaacgcg cgaccaggca atgacgacgg ccagcaccat taaaagtgag ccggaccaca
     1201 ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat caccgtaccg tgatcgctcg
     1261 ggaatgagtc atccgccgca tgatgcagga agttatagcc gatattttcg acaaagggtc
     1321 ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa caggctgacc gccagcgcga
     1381 tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc ccacaaccaa agtaccgcgg
     1441 ccagcaacgg caccacggta atcaaatctt tagcaataaa aatcgccaac gagatcatcc
     1501 acggagccga gtctggcgtc gcgttaataa gagagaatag agagagattc aaattttcca
     1561 gcataacttc ccgacgcaaa gtgattaaaa gggagccaat acaggcaagt cgttgagaat
     1621 aaagtgcagg ttaaactggg taaagcggca tcgtcttatt tccctcaagc ggcctgttta
     1681 cggtgggtga ttgtaacggg cataggttaa ataaaactta aagaaagcgt agctatactc
     1741 gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac aaaattcgct ttctcgttag
     1801 ctgcgctttt attaaactct gcgcgattat tattggcgaa gaaattgcat gcaaaataaa
     1861 ttagcttccg gtgccaggct tggacgtcag gcgttacttt tccctctctg tctggtgctt
     1921 tacgaatttt caacctatat cggcaacgat atgatccaac ccggtatgtt ggccgtggtg
     1981 gaacaatatc aggcgggcat tgattgggtt cctacttcga tgaccgcgta tctggcgggc
     2041 gggatgtttt tacaatggct cctggggccg ctgtcggatc gtattggtcg ccgtccggtg
     2101 atgctggcgg gtgtggttta tcgtcacctg tctggcaata ttactggcgc aaaacattga
     2161 acaattcacc ctgttgcgct tcttgcaggg cataagcttc tgtttcattg gcgctgtggg
     2221 atacgccgca attcaggaat ccttcgaaga ggcggtttgt atcaagatca ccgcgctgat
     2281 ggcgaacgtg gcgctgattg ctccgctact tggtccgctg gtgggcgcgg cgtggatcca
     2341 tgtgctgcct tgggagggga tgtttgtttt gtttgccgca ttggcagcga tctccttttt
     2401 cggtctgcaa cgagctatgc ctgaaaccgc catgcgtata ggcgagaaac tgtcactgaa
     2461 agaactcggt cgtgactata agctggtgct gaagaacggc cgctttgtgg cgggggcgct
     2521 gggattcgtt agcctgccat tgctggcgtg gatcgcccag tcgccgatta tcatcattac
     2581 cggggagcag ttgagcagct atgaatatgg cttgctgcaa gtgcctattt tcggggcgtt
     2641 aattgcgggt aacttgctgt tagcgcgtct gacctcgcgc cgcaccgtac gttcgctgat
     2701 tattatgggc ggctggccga ttatgattgg tctgttggtc gctgctgcgg caacggttat
     2761 ctcatcgcac gcgtatttat ggatgaccgc cgggttaagt atttatgctt tcggtattgg
     2821 tctggcgaat gcgggactgg tgcgattaac cctgtttgcc agcgatatga gtaaaggtac
     2881 ggtttctgcg gcgatgggaa tgctgcaaat gctgatcttt accgtcggta ttgaaatcag
     2941 caaacatgcc tggctgaacg ggggcaacgg actgtttaat ctcttcaacc ttgtcaacgg
     3001 aattttgtgg ctgtcgctga tggttatctt tttaaaagat aaacagatgg gaaattctca
     3061 cgaagggtaa aaaaatgcct gattgatttg tgcgatcagg cattctcgaa ttaatggtga
     3121 tggtcgtcaa tctggtgttc gataaccatc ccttcaccta cgctggcaag atggcgaaca
     3181 taaggatgcg ggcggtaagc cggagctggc gcaggagcca catagacggt ttgcggcaca
     3241 gtcgcgacgc tgaccgcttg tggaacgctg actgaatcag gaaccacaac cactttgtat
     3301 ccactcggca catcgacggt gacactttgc gccatcgcca caccagtaaa gcccattaaa
     3361 agcgcgccca acagtagaca atttttcata atcatttcgc ctgaaataat gcagcgggtt
     3421 aatttccgcg aattatgcag ccttcattca ggcattgtgt tgccgggcgt gctgccgttt
     3481 ttattggtca aatggcgctt cgtgtttaag aactttatcg atcacatcca gtacgccttc
     3541 acggttattg tagcctgccc ggtattttgc cgctgcgacg actgcgctgc cggcattttc
     3601 cattgcaaaa ctaaagcctg cctgacgcag catctcaata tcgttaccgc catcgccaaa
     3661 gaccaccact tcgctgtcgt ctattcccca taatttctgc agttggcgaa ggccattggc
     3721 tttatgtacg ccggggataa tcagatcgat gctgccgttg ccggtgtgga ctggcaccat
     3781 aatatcgccg atggcctcat gtaatgcttt ttgtacttgt ggaatcagtt catcggaaag
     3841 attcaggcca aacttaaaga aaatatcttc taagttgtca aagttatcga cgtattccag
     3901 acggtgataa tacatttccg ccaccgtttt catggcatcg tcatattttt tgagtgtata
     3961 ggcgctattt tttccgcagg caataatttc cacttccggg cgcgtcagca aatgttccac
     4021 gacagtagca aacgcatcct tcgacagctc gccattaaaa acatctttgc cttcactcat
     4081 tacccagccg ccgttttcag ccacaaaggc aatttcattc gcaatttcag ggaagaaaga
     4141 gatcaactga taatattgat tcccgctggc gaccacaaag cgaatgcctt gtgctttcat
     4201 ttgctgatac tgagccataa accgctcacg gttataggtt ttttgatcgc ttaagaaagt
     4261 accatccatg tctaccgcaa ttaatttaat gctcatcaac tattctccat cgccgtctgc
     4321 gttttggtat cgggtttggc gacggctttc gcgacaatag ccgcgagaat aaccagcgcc
     4381 agtacaacca gcattgcact acgtaatcca taatgttcgc cgagatagcc cagcagcggc
     4441 ggcccgacga ggaaagccag ataaccggtc gttgctacca cactgacgcg ggttggtgca
     4501 tcggggccgg tatcgctggc ggcagaaatg gtcagcggga agcccagcga ggcacccagt
     4561 ccccaaagta caacagacac tcctgcgacc caggcgctat cgacaaaaat aatcagccca
     4621 atacccaacg cccccattag tgcactggcc cgaaccacgg caacgcgact gtaactgtcg
     4681 atgaaccaac cgccggtgaa gcgtccaacg gtcatcccca gggtaaaacc ggcataaatc
     4741 agtgagccgg aagtggggct aaaaccgtga ccatcaacca ttaataaggg taaccagtcg
     4801 ttggcagaac cttcggcaaa ggccatcgcc agcactacaa caccaatcaa cagcaactgg
     4861 atatcgcgat aaaaaggaat gcctttttcg ccatgctggg tgccatcggc agcatttttg
     4921 cccgtaccgt cagggattgc ctgaatggcg atataaatag gcgcgatacc taccagcgcc
     4981 gccaataaaa tgtgcaccgt tgccggaacg ccaaaggccg tcagtgccat cccgacacca
     5041 gcgcctgcca gcgtgcccag gctataaaaa ccgtgcatca tcggcaaaac cgttttattc
     5101 atttctcgct caacggcggc accttcaacg tttatcgcca cttccgcaga accaaaactt
     5161 gccccaaaga cgccgagacc aacggcaaag agcaggggcg atgtcagcca gagtgccaga
     5221 cttaatatca tcatcccgat caatgcgcag gacatcgtga ccaggatgac attacgtgtc
     5281 ccaaagcgtt tcactaacca cgccgagcag agaataccgc tcatcgaacc gatcgacaga
     5341 ccaaagagaa caccgcccat ttcagcgatc gagacagaga gaatatcgcg gatagcaggc
     5401 gtacgggttg cccaggacgc cattaacagg cctggcaaaa agaagaacat aaacagcgcc
     5461 caggttcggc gtttcaatgc attacgtgaa gaatttacgg tcatagatca cgtcaaaata
     5521 agaagaggga agacaacatt agcaaggttg tgtacatttg tacacaattg cagacagagg
     5581 aaatgacatg cgtcgcgcta acgatccgca acggcgagaa aaaattatcc aggccacgct
     5641 ggaggcggtg aaactttacg gaatacatgc tgttacgcac cgcaaaattg ctacccttgc
     5701 cggggtaccg ttgggatcga tgacctacta tttttcagga attgatgagt tgttgctgga
     5761 ggcgttcagc agttttactg agattatgtc ccggcaatat caggcatttt ttagcgatgt
     5821 tagtgatgct caaggcgcat gccaggctat caccgatatg atctacagct cacaggttgc
     5881 aacgccggat aatatggagc tgatgtacca gctctacgcg ctggctagcc gaaaaccgct
     5941 attaaaaacg gtaatgcaaa actggatgca gcgcagtcag caaacgctcg aacaatggtt
     6001 tgaacccgga accgcccgcg cgcttgatgc gtttattgag gggatgacgc tgcattttgt
     6061 caccgaccgt aagccgctat cgcgcgagga gattttgagg atggttgaga gggttgcagg
     6121 gtagtagata aattttagat aacaaaaaac ccatcaacct tgaaccgaaa tggcggggtt
     6181 gatgggctcc acaaaatggg gacatcaaag aaaagcagtg gcactaatta agactgatgc
     6241 cctgcggaaa agttctgcgg ttgtgcaaaa aaatttcatt ttcagggcaa cttcagtttt
     6301 atcctaatcc tggccatacc atgacgatga ttgtccctgc cagcgtcagc aggacgttgg
     6361 cgattgcgta ggtgcccgca tagcccagcg cagggatgtt actgcgagct gtatcactga
     6421 tgatctccat tgccggcgcg caggtgcgtg cgcccatcat tgcgccgaac aacagtgcgc
     6481 ggttcattcg caatacataa gcaccgaaca agaaacagat aaccacgggc acaagactga
     6541 caattaatcc ggcaatcaac atctgaccgc caatcgcgcc caggccgtta ttaataccgc
     6601 taccggcgct cagaccaacg cctgccataa acaccatcaa gccgaactct ttcaccatgc
     6661 ttaatgcacc ttgcggaatg taaccgaagg tcgggtggtt agcacgcata aagcccagca
     6721 taattccggc gaataacaac ccggcagcgt tccccatgcc gaaactgaat gtgctgaact
     6781 ggaaggtgat catcccgatc atcagcccaa taacaaagaa ggcgcaaaat gccagcaggt
     6841 cagtgacctg gctgtgaatc gagataaagc cgatgcgatc ggcgatggtt tttacgcgac
     6901 gggcatcacc gctgacttgt aaaacgtcac ctttgttaag cacgacgttg tcatctatcg
     6961 gcatctcaat ctggctacga atgacgcggt taaggaagca accgtgatcg gtcaacttca
     7021 gttgtgcgag acgtttacct acagcgttat ggtttttaac gaccacttct tcagtgacga
     7081 tacgcatgtc gagaaggtca cgatcgaaaa cttctttacc gttacggaag ctgggatcga
     7141 gtcgggcatg ggcgtcggga tagcctacca acgctatttc atcgcccatt tgtagcacgg
     7201 catcaccgtc tggatttgcc agaatcccgt tacgtcgaat acgttcaatg tagcagccgg
     7261 tttgtcgata aatacccagt tcacgcagat ttttgccgtc ggtccaggcc accagctccg
     7321 ggccgacgcg ataggcgcgg atcaccggta aataaacctt acggttggca tcagtgtcca
     7381 ggccacgttc gcgggcgatt tgctgggcgc tggtctgtaa gtcctgatgc tgcaatttcg
     7441 gcaagtaacg cgcaccaaca atcaaactca ccagaccgat taaataggtt aaggcatacc
     7501 cgaggctcag attatccagt gccagtgaga gctgcctgct ttccatgccg gaatgatgca
     7561 gtgtatcgcc agcaccgacc agaaccggtg tcgacgtcat agagcctgct aacataccgg
     7621 ccgtcaggcc aatatcccag ccaaacagct tacctaaccc taaggcgatc accagcgcac
     7681 tgccaaccat caccagtgct aacattaggt aatttttccc atcgcgaaaa aaaatggaaa
     7741 aaaagttcgg tccggcttcg accccgacgc agaaaataaa cagcataaag ccaagattaa
     7801 gcgcatcggt gttaatgctg aaatgttgtt ggcctaataa cagcgatacg actaaaacgc
     7861 caatggaatt acccagttgg atcgaaccaa gtcgtaactt tccgagacat agcccaagcg
     7921 cgaggaccac aaataataac agaatgtaat tcccatttaa caattcggcg acgtttatat
     7981 tcacggaggc taacttcttg tttactagta agctgttgaa agaaatgcta atttacgata
     8041 atgtgtttta ccagaattca gggcgcagat tcattcagcg cacctaaacg atagtaaagt
     8101 aacaatatat tttactagtg taatcacatt aggtatcaac ggctatatga attgcgttgg
     8161 cctatattag catggaatgc gaagcggctt tatcttactg aacgccacac tggcgaaaaa
     8221 tgtgttcgat agacgcagtg tcaggaggaa cgagtgaaac ataaacaacg ttgggcgggg
     8281 gcaatctgct gttttgtcct cttcattgtg gtgtgccttt ttctggcgac gcacatgaaa
     8341 ggcgcttttc gggctgccgg gcatcctgaa atcggcttgc tgtttttcat tcttcctgga
     8401 gcagttgcca gtttcttttc acagcgtaga gaagtcctga aacctctatt tggcgcaatg
     8461 ctggcggcac cctgttcaat gctcattatg cggctgtttt tttcaccgac gcgctcattc
     8521 tggcaagagc tggcatggtt actaagcgcg gtgttctggt gtgcgctggg ggcactgtgt
     8581 ttcttattta tcagtagttt gtttaaacca cagcacagaa aaaatcagta aagccctcaa
     8641 cgcgagggct tgtcagacga tcaggcgtcc agattttctt tcacccatgc agcaaaatcg
     8701 gtatagccgc cgatatgttg ctgatcgaca aaaatctgcg gcacggtttc tacgggttta
     8761 cctgcctttt gttgtagatc ttctttagtg atcccttccg cacgaatatc tacatactga
     8821 tactgaaaat catcgcgttc attgctcaat ttctcagcca gatcttttgc acgcacacag
     8881 taagggcaac ccgaacgacc aaaaataacg gtttgcatta tttctctcct catagattta
     8941 tgcctgtaat gatcacgcta aaatgtattc gctgaaagta ggtttaacct gttgcattaa
     9001 ttgctaaaag ctataactgt taaacacaat acagtgaaaa gttttagact gaaggctcac
     9061 tttgcagagg gaagcgtatg cgcgcgatcg gtaaattgcc taaaggcgtg ttgatactgg
     9121 aatttatcgg aatgatgcta ctggcggtgg cgctgctgtc ggtaagcgac tccctgtcgc
     9181 tgcctgagcc attttctcgg ccagaagtgc agattctgat gatttttctc ggtgttttgc
     9241 tcatgcttcc cgctgcggtg gtggttattc ttcaggtggc aaaacgtctt gccccacagc
     9301 tgatgaactg tccaccgcaa tattcacgtt cagaaagaga aaaagataat gacgccaacc
     9361 attgaactta tttgtggcca tcgctccatt cgccatttca ctgatgaacc catttccgaa
     9421 gcgcagtgtg aggcgattat taacagcgcc cgtgcgacgt ccagttccag ttttttgcag
     9481 tgtagtagca ttattcgcat taccgacaaa gcattacgtg aagaactggt gacgctgacc
     9541 ggcgggcaaa aacacgtagc gcaagcggcg gagttctggg tgttctgtgc cgactttaac
     9601 cgccatttac agatctgtcc ggatgctcag ctcggcctgg cggaacaact gttgctcggt
     9661 gtcgttgata cggcaatgat ggcgcagaat gcattaaccg cagcggaatc gctgggattg
     9721 ggcggggtat atatcggcgg cctgcgcaat aatattgaag cggtgacgaa actgctgaaa
     9781 ttaccgcagc atgttctgcc gctgtttggg ctgtgccttg gctggcctgc ggataatccg
     9841 gatctcaagc cgcgtttacc ggcctccatt ttggtgcatg aaaacagcta tcaaccgctg
     9901 gataaagacg cactggcgca gtatgacgag caactggcgg aatattacct cacccgtggc
     9961 agcaataatc gctgggatac ctggagcgat catatccgcc gaacaatcat taaagaaagc
    10021 cgcccattta ttctggatta tttgcacaaa cagggttggg cgacgcgcta aaaccgccac
    10081 gtcgatgtat gatacgcggg cttttgacca ggtctgacag agaggtgcag ggtgaaaatt
    10141 gccatattgt cccgggatgg aacgctctat tcgtgtaagc ggctgcgtga agccgctata
    10201 cagcgcggtc acctggttga aattcttgat ccgctttctt gctacatgaa cataaatcct
    10261 gcggcgtctt ctatttacta caaaggccgc aagttacccc attttgacgc agtgatcccg
    10321 cgtattggca ccgccattac cttttatggg acggcggcac tgcgccagtt cgagatgctg
    10381 gggagctatc cgctcaatga gtcggtcgcc attgcccggg cgcgtgacaa attgcgttcc
    10441 atgcaactgc tggcgcgtca gggcatcgac ctgcctgtca cgggcattgc gcattcgccg
    10501 gatgatacca gcgatttaat cgacatggtc ggtggtgcgc cgctggtggt caagttggtt
    10561 gaaggcacgc agggaattgg cgtcgtgctg gcggagactc gtcaggcggc ggaaagcgtg
    10621 attgacgctt tccgcggtct gaacgcgcat attctggtgc aggaatatat caaagaggcg
    10681 caagggtgcg atatccgctg tctggttgtt ggtgatgaag tggtcgctgc gattgaacgg
    10741 cgggcgaaag agggctattt tcgttccaat ttgcatcgtg gcggcgcggc aagcgtcgcc
    10801 agtatcacac cacaggagcg tgaaatcgcg ataaaagccg cgcgaacgat ggcgctggac
    10861 gttgctggtg tggatattct gcgtgctaat cgcgggccgt tggtgatgga agtgaatgcg
    10921 tcgccggggc tggaaggaat agaaaaaacc accggtatcg atatcgcggg taaaatgatc
    10981 cgctggatcg aacgccacgc tacgacagaa tattgcctga aaacgggtgg ttagtcgcaa
    11041 tcacattact gatcatggtt ttgcctgcgc tttttgcgta agctgtgccg gtctttttat
    11101 cgaaagaggt tgtacaaaat tatgacatcg ctggtcgttc ctggtctgga tacgctgcgt
    11161 caatggctcg atgacctggg gatgagtttt tttgaatgtg ataactgtca ggctctgcat
    11221 ctgccccata tgcagaattt cgacggtgtc tttgatgcca aaatcgatct gatcgataac
    11281 acgatcctgt tttctgccat ggcggaagtc cgaccttcag ccgtattgcc gctggcggcg
    11341 gatttatctg ccatcaatgc cagttcgctg accgtgaaag catttcttga tatgcaggat
    11401 gataatctgc caaagctggt ggtttgccag tctttatccg ttatgcaggg cgtaacctat
    11461 gagcagtttg catggttcgt gcgtcagagc gaagagcaga tttcgatggt cattcttgaa
    11521 gctaatgccc atcaactgct gttaccgact gacgatgaag ggcaaaacaa cgttaccgaa
    11581 aactattttc tccactgata attcctttcg agcacgcagt cgctgccgca gtgactgcgc
    11641 gctgcaaaat tatctgctgt ttttaacctt ttcttaaaga ttatttcact tctcttgtgt
    11701 cgatttggct ttatcacata gagcaaatac gcataaaaat ttgttaaata ccgtttttta
    11761 atccgagcta tagtctcaaa ccctggctaa agttattctt gcgatgcttc tatatagcga
    11821 gcagtgctgg ccgggagaga gttctctttt cttacaccgc gccgataaaa aaatatgcac
    11881 gtttattgca tatctttcag tgtgacaact tttgttcgtt tgttaacgaa ctttcagaag
    11941 gaaagagatg tgaccgcctt aaataaaaaa tggctatcgg gtctggttgc gggtgctctg
    12001 atggccgtct ctgtcggcac gctcgcggct gaacaaaaaa cactccacat ttataactgg
    12061 tctgattata tcgcccctga cacggtggcc aattttgaaa aagaaaccgg tattaaagtc
    12121 gtctacgatg ttttcgactc taacgaagta ctggaaggca aattaatggc gggtagcacc
    12181 ggctttgact tggtggttcc atctgccagc tttctagaac gccagttgac tgcgggagtt
    12241 ttccagccgc tggacaaaag caaattgccg gagtggaaga atctcgaccc ggagctgctg
    12301 aagctggtcg ccaaacacga tcccgacaat aaatttgcta tgccctatat gtgggcgacg
    12361 accgggattg gctataacgt tgataaagtt aaagctgtgc tgggcgaaaa cgcgcccgtc
    12421 gatagctggg acttgatcct caaacctgaa aatctggaaa aactgaaaag ctgcggtgtc
    12481 tctttcctgg atgcgccaga agaagttttt gctaccgtgt tgaattatct cggcaaagat
    12541 cccaacagca ctaaagcgga tgattacacc ggaccggcaa ccgatctgct gttaaagctg
    12601 cgcccgaaca ttcgttattt ccattcatct caatacatta acgacctggc aaacggcgat
    12661 atttgcgtcg ctatcggctg ggcaggtgat gtctggcagg cgtcaaaccg cgcgaaggaa
    12721 gcgaagaatg gagtgaatgt ctcgttctcg attccaaaag aaggggcgat ggcgttcttt
    12781 gatgtattcg ccatgccagc ggatgccaaa aacaaagacg aagcctatca gttcctgaat
    12841 tacctgctgc gcccggatgt agtagcgcat atttccgacc atgtgttcta tgtcaacgcc
    12901 aataaagcag ccacgccgct ggtgagtgcg gaagtccgtg agaacccggg tatttatccg
    12961 cctgcggatg ttcgtgcgaa gctgttcact ctgaaagtgc aggacccgaa aatcgaccgt
    13021 gtgcgcaccc gcgcgtggac caaagtgaag agcggaaaat aatccgcagc cttagatgcc
    13081 gggcgggcgc accacacccg ccggcaattc gcaccatcat ggtgcgcttg cacacattca
    13141 atgccggaga gcagccgtga atgacgctat ccctcgcccg caggcgaaaa cccgtaaggc
    13201 gctgacgccg ctattagaaa tccgcaacct caccaaatcc tacgatggtc aacatgcggt
    13261 ggatgatgtc agcctgacca tctacaaagg tgaaatcttc gcgctgctgg gcgcatccgg
    13321 ctgtggcaag tccacgctgc tgcgtatgct ggcaggtttc gaacaacctt ctgccggaca
    13381 gataatgctt gatggcgtcg atttgtcaca ggttccgcct tacctgcgcc ccatcaatat
    13441 gatgtttcag tcttacgcgc tgtttccgca tatgaccgtg gaacagaaca tcgcttttgg
    13501 cctgaaacag gacaaactac cgaaagcgga aatttccagt cgggtcaatg agatgctcgg
    13561 cctggtgcac atgcaggagt tcgccaaacg taaaccgcat cagctttctg gtggtcagcg
    13621 acagcgtgtg gccctggccc gaagccttgc caagcgcccg aaactattac tgctcgacga
    13681 gccgatgggc gcgctggata aaaagctgcg tgaccggatg cagcttgaag tggtggatat
    13741 tctggagcgc gtcggtgtga cttgtgtgat ggtcacccac gatcaggaag aggcgatgac
    13801 catggcgggg cgcatcgcca ttatgaatcg tgggaaattt gtccagattg gcgaaccgga
    13861 agagatctac gagcatccga ctacccgcta tagcgccgaa tttattggct cggtaaacgt
    13921 ctttgaaggc gtactcaaag agcgtcagga agatggcttg gtgcttgatt cgccggggct
    13981 ggtgcatcca ctgaaagtcg acgcggatgc ctcggtggtc gataacgtgc cggtacatgt
    14041 ggcgctgcgc ccggaaaaaa tcatgctttg cgaagagccg cccgccaatg gttgtaactt
    14101 cgcggtgggg gaggtgatac acattgccta tctcggcgat ctttcggtgt atcacgttcg
    14161 tctgaaaagt gggcagatga ttagtgccca gctacaaaac gcccatcgtc atcgtaaagg
    14221 gttaccgacc tggggcgacg aagtgcgttt gtgctgggaa gtggacagct gtgtggtgct
    14281 gacggtttaa ggagcaaaga tgaatacact tgaacctgct gcccagtcga aaccgccggg
    14341 cggatttaag ctgtggttgt cgcagctgca aatgaagcat gggcgcaaac tggtcattgc
    14401 gttgccatat atctggttga tcttgctgtt tctgctgcca tttctgattg tctttaaaat
    14461 aagcctggcg gagatggcgc gcgctattcc accttatacc gaactgatgg agtgggctga
    14521 cgggcaactg tccatcactc ttaatctcgg taattttctg caactgaccg acgatccgct
    14581 ctatttcgat gcttatctcc agtcgctaca ggtggctgcg atttcgacat tttgctgttt
    14641 actgatcggc tatccgctgg cgtgggcggt ggcgcacagt aagccttcga cccgtaatat
    14701 tttattacta ctggtgatcc tgccgtcgtg gacctcgttt ctgatccgcg tttatgcttg
    14761 gatgggaata ttaaaaaaca acggtgtgct gaataatttt ctgctgtggc tgggggttat
    14821 cgatcaaccg ctgaccattc ttcataccaa tctggcagtt tatattggca ttgtttacgc
    14881 ctacgtgccg tttatggtgc tgccgattta taccgcgttg attcgtattg attattcgct
    14941 ggtggaagca gcgctggatc tcggtgcacg accgctgaaa acgttcttta ctgtgatcgt
    15001 gccgctgact aaaggtggga ttattgccgg atcgatgctg gtgtttatcc cggctgtggg
    15061 cgagtttgtg atcccggaac tgctcggtgg cccggacagc atcatgatcg ggcgcgtgct
    15121 atggcaagag tttttcaata accgcgactg gccggtggcc tcggcggtag cgatcatcat
    15181 gttgctgctg ctaattgtgc cgataatgtg gttccacaaa caccagcaaa aaagcgtggg
    15241 agaacacgga tgaataattt accggtagtt cgttcgccct ggcggattgt gattttgctg
    15301 ctgggcttta cctttctcta cgcgccaatg ctgatgctgg ttatctattc gtttaacagc
    15361 tcgaagctgg tgacggtgtg gggcggctgg tcaacgcgct ggtatggtga gttattgcgc
    15421 gatgacgcga tgatgagtgc ggttggttca agcctgacaa ttgctgcctg tgcggcaacg
    15481 gcggcggcga tcctcgggac tattgcggcg gtggtgctgg tgcgctttgg caggtttcgc
    15541 ggatcaaatg gctttgcctt tatgatcacc gcgccgctag taatgccaga tgtcatcacg
    15601 ggcttgtcgc tgttgttatt attcgtcgcg cttgctcatg ccattggctg gcctgcggac
    15661 cgcggtatgc ttaccatctg gctggcgcat gtcactttct gtacggctta tgtggcggtc
    15721 gttatttcgt cgcgtctgcg ggaactggat cgctcgatag aagaagccgc gatggatctc
    15781 ggtgcgacgc cgctgaaagt gtttttcgtc attacgctac cgatgatcat gcccgcgatc
    15841 atttctggct ggttactggc ttttactttg tcgcttgatg atctggtgat cgccagcttt
    15901 gtttccgggc cgggagccac cacgttaccg atgctggtct tttccagcgt gcggatgggg
    15961 gtgaatccgg aaatcaacgc cctggcaaca ttgatcctcg gcgcggtcgg aattgtcgga
    16021 tttatcgcct ggtatctgat ggcgcgcgca gaaaaacagc ggatacgcga catccaacgt
    16081 gcaagacgtg gctgaagaca ctaaaatttg ccaacctggc tacataatgc cgcgcatgtc
    16141 gcggcattgt tttcatggaa gacgaaacgt tgggattttt taagaaaaca tcttcatctc
    16201 atgctcgcct gaatgtgcct gcgctggtgc aggtggcggc gctcgccatt attatgatcc
    16261 gtggcctcga cgtgctgatg attttcaata cgctgggcgt gcgcggtatt ggcgagttca
    16321 ttcatcgcag cgtacaaacc tggagtttaa cgctggtctt tttaagcagt ctggtgctgg
    16381 ttttcattga gatctggtgt gcgttttcac tggtgaaagg gcgtcgctgg gcgcgctggc
    16441 tatatctgct gacacaaatc accgccgcaa gttacttgtg ggcggcttcg ctggggtacg
    16501 gttatccgga gctgttcagc attcccggtg aatcaaaacg tgaaatcttc catagcctga
    16561 tgctgcagaa gctgccggat atgctcatcc tgatgctgct gttcgttccc tcgaccagtc
    16621 ggcggttctt ccagttgcaa taatgtgtat aatcgtcgcc cctgatgatg tgaaggtcaa
    16681 tgtatgcagt gcgcacttta cgacgcgggt cgctgtcgtt cctgtcagtg gataacgcag
    16741 ccgattccag agcaactctc cgctaaaacc gccgatctta aaaatctgct cgccgatttt
    16801 ccggttgagg aatggtgcgc gccggtgtca ggcccggaac aagggtttcg taataaagcc
    16861 aaaatggtgg tgagtggtag cgttgaaaaa acactgctcg gtatgctgca tcgcgatggc
    16921 acgccggaag acctttgtga ctgcccgctt tatccagcct catttgcgcc cgtttttgcg
    16981 gcgctaaaac cgtttatcgc ccgagcgggg ttaacgccct acaacgtggc gcgtaagcgt
    17041 ggcgaactga aatacattct gctgactgaa agccagagtg atggcggcat gatgctgcgc
    17101 tttgtactgc gttctgatac caagctggcg caactgcgta aggcgctgcc gtggttacac
    17161 gaacaactac cgcagctgaa agttattacc gtcaatattc agccggtaca tatggcgatt
    17221 atggaagggg agacggagat ctacctgacc gaacaacagg cactggcgga gcgttttaat
    17281 gacgtaccgc tgtggatccg tccgcaaagt ttcttccaga ctaatccggc ggtcgccagc
    17341 cagttgtacg ccaccgcgcg cgactgggta cgacagctgc cggttaaaca tatgtgggat
    17401 ctgttctgcg gtgtgggggg ctttggttta cactgcgcga cgcctgacat gcagttaacc
    17461 gggatcgaaa ttgcgccaga ggccattgcc tgtgcaaagc agtcagccgc tgaactgggc
    17521 ttaactcgtt tacaatttca ggcgctggac tccactcagt ttgccactgc tcagggggag
    17581 gtgccggagc tggtgctggt taacccgccg cgccgcggca ttggtaaacc gctgtgtgat
    17641 tatctttcaa cgatggcacc gcgttttatt atctactcca gctgtaacgc ccaaaccatg
    17701 gcgaaagata tccgcgaact gccaggttac cgcatcgaac gggtacagct ttttgatatg
    17761 ttcccgcaca ccgcgcacta tgaagtgttg acgctgctgg tgaagcaata aaaaagccac
    17821 aggtgcggct tcagattgct gatcaggtgc gctttgttca tgccggatgc ggcgtgaacg
    17881 ccttatccgg cctacaaaat catgctaaat caatacattg cagagttgat gtgggccgga
    17941 taagcgcagc gcatcaggca atcttacttt tgtcatcagt ctcaattact gcggaaacca
    18001 ctggtcattg attttctgat atgtaccgtc agctttaatt gctgccagcg cgttattcag
    18061 tttttccagc agagctttgt tatccggacg tacagcgata cccaggccgg tgccgaagta
    18121 ttgcggatcg gtcacttttt cagtcgcaac gcccagttgc ggattggttt tcagccattc
    18181 gtttaccacc gcggtgtcac caaatacccc atcaatacga ccatttttca gatcgataaa
    18241 ggcattctga taactgtcat aggagacagt tttcacttcc gggtgctgat cctgaatata
    18301 tttctggtgc gtcgtaccgt tttccatccc aatgcgtttg cctttcaggt cggcaaacgt
    18361 tttgtaggta tcttttttgg aaatcacgac ggctgagttt tcatagtagg gtgtggtaaa
    18421 cgaaacctgc ttgctacgct ccggggtgat atccataccg gagattacgg cgtcatattt
    18481 tctgaatttc agtgacggga tcaggctgtc gaacgcgtga ttagtaaaag tacattctgc
    18541 ctgcatttgt ttgcacaagg cttttgccag atcgatatca aagccgacaa tctcattatt
    18601 agcacctata gattcaaagg gtggataggt ggctgaaacg ccaaaattga ttttctctgc
    18661 ggcagaagca ccgaaagtaa aggaagcaag taaagcggca agaactaact ttttcatgat
    18721 ggaactcccg tctgtcaatc ttatgatttt tggccgtgtc tgcggcatgg gataacaatg
    18781 ccatcaagtg aatttatatg caataaacat gattaaataa tttaaatgaa ataaaaaaag
    18841 acggataact tagtgagttg tccgtcttca ttataagaat ttatgcacta tgtaggccgg
    18901 ataaggcgtc cacgccgcat ccggcacagg caccgtgctg atgtctgatg cgacgctggc
    18961 gcgtcttatc agacctacaa acccccccgg cgaatgtacg cagctacatt aattccgccg
    19021 ttcgaatgcc agtgctttgc gctcgatcag acgcatcatc agcgtcagca ggccgttgac
    19081 gaccaggtaa ataatccctg ccgcaccgaa caccattaca tcgtaggtgc gtccgtacaa
    19141 caactggctg tatcccatca cttccatcag cgtaatggtg taagccagag aggtactttt
    19201 gaacaccagc accacttcgt tggaataaga agagagcgag cgtttaaagg catacggcag
    19261 caggatcgcc agcgtatctt ttttgctcat tcccagggcg ctacaggact gccactgacc
    19321 ttctgggatc gcacgaattg caccgtaaaa cagctgcgtg gtatacgccg cactgttcag
    19381 cgataacgca atcagcgcac ataaccacgg ttctgacaac aaatgccaca gtgccggata
    19441 ctcctgcaac gtcggaaact ggcccggccc gtaataaatc aggaagatct gcaccagcag
    19501 cggcgtaccg gtaaacagcg tgatataacc ccgcaccagc cacaccagca ccggcgtttt
    19561 cagcgtcagg atgatggtaa aaatcaatgc cagaatcagt gccacaatca gcgaggcaac
    19621 ggttagcgtc aggctggtgt gcagcccttt catcagctcg ggtaaatact caaacattag
    19681 ctgggcctcc gctcaaaacg tgtcgcgcgc aggtcaatgc gtttgagaat gtactgactg
    19741 agcagggtga tcaccaggta aatcgccgcc gccacaatgt accaggtaaa tggttcctgg
    19801 gtacgagtag caatgctttt ggtttgtaac attaaatcat tcacactaat caaactgacc
    19861 agcgcggtat ctttcagcag caccagccac tggttaccga ggccaggcag cgcatgacgc
    19921 cacatctgcg gcatcaccag acggaaaaag atagccgatt tcgacagccc cagcgcctga
    19981 ccagattccc actggcccac cggtacagct ttcaacgcgc cgcgcagcgt ttgcgaggca
    20041 taggcggcat acagcagtga cagagcgatg acaccacaaa ggaacggact cacgtcgaag
    20101 ttctcaatgt ccatctgcac cgggatctgc acgaacccaa gattgatagt gaagccatcc
    20161 gaaagcgtca gcagtaactg cgaggagcca aaatagataa acagcactac cagaatttct
    20221 ggcaggccac gcagaatggt taccagcgct gaacctgccc acgcgacagg acgccatttc
    20281 gctgactccc ataccgcaaa gaacatcgcc agcgccagcc cgacaatcaa tgcacaaacg
    20341 gcaaggccga cggtcatccc ggcggcgctt gctaaaggaa aaaattcatt catcaggaat
    20401 tacttctgga accatttgtt gtagatggtt tcgtaagtgc catctttctt cactttttcc
    20461 agcgcagtgt tgagtttctg ctgcagttca gtgtttccct gacgtaccgc gatgccgagg
    20521 ccagtaccga aataatcttt atcggtcact ttatcgccca ctgccgccag ttttgggtta
    20581 tctttcagcc actcagtgac cactgcggtg tcaccaaata cgctgtcgat acgcccgttt
    20641 tgcagatcca gttttgcgtt ctggtagctg tcatacggaa cggtggtgat ttccgggtgc
    20701 ttatccataa tgaacttctg gtgtgtcgtc ccgttctgaa cgccgacttt tttgcctttc
    20761 agctgatcaa cactggtgta tttgccttgc tgacccacaa acagggcaga gttgtcatag
    20821 tacggggtag taaacagtac ctgcttttca cgctccggag tgatatccat gcccgccatc
    20881 actgcttcta cgcgacggaa tttcaggctt gggatcaggc tgtcaaacgc ctggttagag
    20941 aaagtacagg ttgcatcaat ctctttacac agtgcctgcg ccaggtcgac gtcaaaacca
    21001 acgatctggt tgtttgcatc aatcgattca aacggaggat agggggcttc ggtagcaaaa
    21061 cgaatggttt cggcagctgt ggcggaaaga ctaaaacctg caattaacgc ggcaatcaga
    21121 acttttttca ttgttgttat cccgaatctt agtgagagag atagttttta aatgcttcgg
    21181 tttgcggctc ggtaaagcag ctcgcgtcgc cttgttctac gatatgacca ttttccatat
    21241 acaccactcg gctggcggtt ttacgcgcca cttcaacttc gtgggtgacg atcacctggg
    21301 taatattcgt ttctgccagc tcacgaatga tgctgacgat ttgtgccgta atttccgggt
    21361 ccagtgcggc ggtcggttca tcgaacagca gtacctgcgg ttccatcatc aacgcacggg
    21421 caatagcaac acgctgctgc tgaccaccag aaagatgcag cgggtaacga tcgctataag
    21481 gtttgagacg cagacgttcc agcagttttt ctgcacgggc cagcgcctga tctttactca
    21541 accccagtac acggcagggc gcttcaatca ggttttgctg cacggtcaga tgcggccaca
    21601 ggttgtattg ctgaaacacc atgccaacgt tacgacgcaa atcgcgaatc gctttgtcag
    21661 agggtgtttt ggtgaaatcg aaatggttgc ctgcaatgtt gagcgtaccg gagcgcggca
    21721 tctcaagcag attgagtaca cgcagcagcg agcttttacc cgcgccgctg gggccaagta
    21781 acaccagcgt ttcgccctgt gggcaatcca gcgtgatatc gaacagcgcc tgatgcgcgc
    21841 cgtagaagca attaatgccg tttaattgaa tactcattga cactcgtata ctggcagtct
    21901 gatagctatt gaggtcgaag atagtacctt tgacagaata attatgcaat atttatgctt
    21961 taaaagttaa aagcaaagcg cattattcaa taaacatagc acaaaataac gggggcggtg
    22021 gtcggtgagc ataaatgtcg gcattcctca cgaaatgccg gataatttac ggggtttatt
    22081 ggttgatcaa ggcgttagcg attctcgatg gactgacgga gcgtacccgc cgtggcatga
    22141 acgctaccgc ctaagtagcg cacatcgtcg atgacccaac actggccttc ctgaatcatt
    22201 aacacttcat cctgccagcc ctggtcaccc tgtttgagat ccacccgcaa tggaatgtta
    22261 cgggcatcac gattagggat agtcgatgca ctggcaacgt gggcgctgtc tggcaaggtg
    22321 gttcgactgg agaacggatc gctgcttaac aattcacgat gactattatc gcggctggca
    22381 tcgctaagtt gggttgccag tttgtcactc aaataggggc gcagggcggt aatgtcattg
    22441 ctgcgatgtt gaatgcggta gtcgtagaat tgttgagcca cactgtcagg ccctccctgg
    22501 acacaggaac cggtgcgggg gccgttatct ttataagctg gcgtgacggt ggtacaggca
    22561 ctcagcagca gtgcacatgg aataagtaga gatagtgaac ggtagcgcat aatcatttcc
    22621 ttattagctt gctgtctgcg tcacactacg accaaacgct atcagattat ttaatacatt
    22681 gtttttaata caattctgaa acaatctgaa cacctgattg ggtttgagtc cattgctggc
    22741 gacccactca aggttgctcg tagctgactt acttaaatta cccaaacaat agacagtgta
    22801 aaccatttgt gaggttgagc gtgttatatg gctgaaatga tcatttaagg taaatatgat
    22861 agttctgaaa tggtggtata aatcctcctg tagaggttaa gagcatctgc aagaagccag
    22921 tttcacattt ataacgcgtg ataaatgaat gcggttattg tctaatcatt acaaaatttt
    22981 tcacatgcga taagagatcc tgcgattaag atgattgcca aaattacgtt gtatttttga
    23041 aaatgagcga aatttgatag tcccagcttt gactctcgat aatattattt atgctacaat
    23101 tgtgggtaaa gaatttaagc taatgaattt gagtgaagaa taaattaaat tgcttcagta
    23161 tttcgttaat taatataaat acaaaaggcg ttgtgctttt ataattataa agcgcgagga
    23221 gttcggtgga ttatcgtttt tcttgggtga tttttaatat gataaacaat atatctgatc
    23281 aagcatcatc atttccaggc acacaactaa atcaaagtga taatttcctg gattcactga
    23341 gggaattttt cgcgatatta aatcccagtc gtaagggtga gttgtctaca tgggatacca
    23401 tctacttgca tctgatatta gcaataaatg ctgacagtga tctcataaaa aatgatgtgt
    23461 tgctcgcgga aaatattcca tcagcgaatt accaatttaa tacatttttt agcaatactt
    23521 tcgagattga tgttaaaaaa tatctgggta aatcagaaga taacgaagtt gaaataaaag
    23581 cgggtaatga aagaatctct attggaatac gtaatgtatc gaatggaaaa cttgaaagac
    23641 aacagtttct tttcccactg gactatgaga ataaattaca agaacaatta gataagtatt
    23701 ttacgattga atcgcaccca ttattataca ggtataccat tggtagtaag attgcgaatg
    23761 tgatttttga aaagttatac agtcgtattg actttaacaa agaacaatat atatccttta
    23821 taaaggacgc atttattcat ttttatgatt atagcaggcg atatgctatt agtgagaata
    23881 tcgataaaga cgctgtaaca aacaatatcg cattgatgag cactttttat gacagtgata
    23941 atacgtcagg tgaggtttta aataatgatt ttacagaaga agagtcattt gaaactgcgt
    24001 tagatgtaga gcatgcaata gtgcttggat ttgctgatga taattttgaa acaaaacctg
    24061 ttcattatca ggatttatta acacgattta gtgcctttca agataccgtg tttaatctgt
    24121 ttcctgaaat gcactcttcg cattaccacg atatttgtag cgtaagtgtg gatatgacaa
    24181 agggaacgca atgcatgatt cacttgatgg tgaatgaaga agttttcatg tctctgcctg
    24241 ttccagttgc cacgatggta cgtgaagatg caagtaattt agtcaatctt aaaacactac
    24301 tcaatgatgg ttgtttcatt aaatatagtc attttaatga cgtggcatta ataaagcaaa
    24361 acataagtaa tctctattta tcacacactg ttgtgaatga aagcatattg aaaaaatgtt
    24421 gttttgaaaa cggcagttta ggtgatgtta aaataacaaa tagcaatgta ataaacagtg
    24481 cattcaaaaa tatttcgttt agatctgtta aaattaataa cgtaaatact cattctttaa
    24541 aatttattaa ctgcaatttt ttcaatgttg atatgataag agttaatctc agcaaatgtc
    24601 tttttcatga atgtagtatg catggtgtca agataaaacc ctggttgcct gtaaaatgga
    24661 caaaagaatt aataagtgat tatttgtatg gctgtttact atctctgtac tctatctgtg
    24721 caagggatat atataatatg aacgccggga ataacgtgaa ggttgctgcg gatgctttcc
    24781 ttgaaattat tttctctctt aaaaataaat attgtattaa actattaagc gctcaggatc
    24841 gggcgtttat atacgagttt gcaagaatga tcttcgctta tattaatgac aaaagcattg
    24901 agatcttatt actttcttgt tttgcggcgg ctgaccaaaa agcaatccag cggtatagac
    24961 ctcagtcaca agatggggaa gactttagga gtcatttaca atataagttg cctctttccg
    25021 ctcattaatg ttgttggtgc aacataataa gacaggtaag cggtacccta taatgaaacc
    25081 aatatcgggt tatgcaacgg ataatcgtaa agacttaagt gggtgttttt gacacaatga
    25141 gatgatttcc taaactgatc aaaaccttct tcccaacatg gcatatcagt ccgataatgc
    25201 tttagagatc gaaggcttaa caaacaagga gatcgatcat gcaattttct acaaccccaa
    25261 ctctggaagg cctgaccatc gttgaatatt gcggtgtggt gaccggcgaa gcgattttag
    25321 gtgccaatat tttccgtgat ttctttgccg gtatccgcga tatcgttggc ggacgttccg
    25381 gtgcgtatga aaaagaactg cgtaaagcac gggagatcgc ctttgaggaa ttaggctccc
    25441 aggcgcgggc gctgggggcc gatgccgtag ttggtattga tatcgactac gaaacggtcg
    25501 ggcaaaacgg cagtatgctg atggttagcg tcagcggtac ggcggtgaaa acgcgtcgat
    25561 gagaagagtc ttctggctgg tcgctgccgc tctgttactg gcagggtgtg caggcgaaaa
    25621 aggcattgtc gagaaagagg gatatcagct tgatacccga caccaggcgc aggcggcgta
    25681 tccgcgcatt aaagtgctgg tgatccacta caccgcagat gattttgata gctcgctggc
    25741 gacactgact gataagcagg tcagctcgca ttatctgatc cctgcggtgc caccgcgata
    25801 caacggtaaa ccgcgcatct ggcaactggt gccggaacaa gaactggcct ggcatgcggg
    25861 gattagcgcc tggcgcgggg caacgcgcct taacgacacc tctattggca ttgagctgga
    25921 aaaccgtggc tggcaaaaat catccggagt gaaatatttt gccccgtttg aacaggcaca
    25981 gattcaggcg cttattccgc tggcgaaaga tattattgcc cgttatcaca tcaagccgga
    26041 aaacgtagtg gcacatgcgg atatcgcacc gcagcgcaaa gacgatccgg ggccattatt
    26101 tccctggcag caactggcgc agcaggggat tggtgcctgg ccggatgcgc agcgggtgaa
    26161 cttttacctt gccgggcgcg cgccgcacac accggtagat accgcgtcat tgctggagct
    26221 tttggcgcgc tacggttatg acgtaaaacc tgatatgaca ccgcgcgagc agcggcgcgt
    26281 gattatggca ttccagatgc atttccgccc gacgttatat aacggcgaag cggatgcaga
    26341 aactcaggcg attgccgaag cattgctgga gaaatacggg caggattagc gcggcagttt
    26401 tccgtggtcg cgtagccagg cggcagtttt ctcgatgcct tcatccaggg tgatgaccgg
    26461 ctgataacct aactcttcct gcgcacgcgt aatatccagc gtaaagtcaa aattcaactt
    26521 ggaggcgccg tagtgggtca gcggcggctc ttttgctgac ttgcggccta aacgctccat
    26581 gctgcgggcg atcatatcca gcatcgggta agggacggaa cgaatacgac agtcaatatt
    26641 caactcgtcg atcagcttct gcacgatgct gcgcagtgtg cgatgctcgc cgttggtgat
    26701 gttgtacaca cgcccggaag gtagcttatc gcaggcttcc tggcttgcca gccacattgc
    26761 gtgcacggca ttttcatagt aggtcatatc gaccagcgca ctgccgccat gcggtaacag
    26821 aatactgccg tagtggtgca tcatatgcgc cagacgggga ataaagactt tatcgtgcgg
    26881 tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt tgtggattcg cctgcgaaag
    26941 catattgatc acttcttcgc tggctgcttt gctgcgggca aactcgttgg cgaagcggtg
    27001 agggcgaaaa tcttctttaa tattgcgatg gtggtgataa tcgaagtaca gggaggggga
    27061 agagatatga ataaagttac gcacacccca ggcgacagcc cattcaccca ggcggcgagt
    27121 ggcgcgaacg ttagccagat cgaaagcctg ttgtgtcccc cagggtgagg taaagctgga
    27181 gcagtgccac agcgtatcaa tgcccgcgag catcacttta gcttgtgatg aaaccagctc
    27241 ggtcagatcc gtcggaacaa actctgcgcc cattttttcc agcaatttac ccattgcttc
    27301 gttgcgaccg ctcgcacgca cgctgatgcc tttctggcat aaaaactcta ccgcgtttcg
    27361 acctaagccg ctggtggcgc cggtaaccag taccttcata tcaatccact gttgttgaga
    27421 aaatgacgtg cgcattcttc cgtgatttcc cccatgatgc aatgggaaac atgaaagaat
    27481 aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca gtctggcgat ttgttttgcc
    27541 attccgcgaa aaataaacag atgcgcgggg atcatcaata accagtaaaa cagccccggc
    27601 ataccgtgcg gatgccagaa agcgcggaca tcgatagtac gatagtcacc tttatcttcc
    27661 agggtaaaac acagtcgtcc cagccccggc gctttcatgc caaataacaa cgtaagttgc
    27721 tttttcggtt caacgacaat cactttccag ctatccaccg catcgccggt ctgtaaatat
    27781 tcgcgctccg ggcggccttt cgccagatta tgaccgatcg cgcggtccat caacgcccgt
    27841 gtctgccaca aaatattgcc aaagaaataa cgctctttac cgccgatttg gttcaccacc
    27901 agccataaag ccgcaaggct ggcggacgtt ttaacggtaa accccgcctg tttggcgaaa
    27961 taaccgtact ccggtcgcca gcgggcaaag gcctgagcgt cgtagcccca gtcgctggag
    28021 ttgaccagtt tttcttcctc tttcaacgtg ctacgtaccg cgtcatcgaa agcgatcagc
    28081 cgttgtggga tgagtgcacg tagcgcggta tcatctgcca gcagatcgtg tttcagcccc
    28141 tgaatcaacg ccctggcggt ggtgggcggt acggaagtaa tcacattgag aaaccacacc
    28201 gaaatccagc gggtggggag ggggatgggg atcaaccagc ggcgcttacc gctcaccgcc
    28261 ataaaatgtt caaactgttg ctgataactg agcacctctg gtccggcggc ttcgaagatg
    28321 cggtgttcgt tggctggatg gtctaacagc gccaccagat agtgcagcaa gttttccagc
    28381 gcgatgggcg tggtgcgtga acgtacccag cgtggcggcg ttaacactgg caggttgtag
    28441 accatatcgc gcatgacttc gaacgccgct gaacctgcgc caacgataat tccggcccga
    28501 agttcggtca ctggtacatt cgcttcacga agaatgtccg ccgtagcctg acgagctcgc
    28561 agatgatccg actgctcatg tggcggggcc tgcaacgaac tgagaaagat taattgctta
    28621 actggtactt cacgtagcgc atcgcggacg ttgagagcca gctggcgctc ctgagcgata
    28681 aaatcgccgc cttcgcccat gctgtgcacc agaaaataga cggtatcgat atcctgcaac
    28741 agggccggaa ggttatccgg ccagctgaga tcgactttat ggcaactgac gtttgccagt
    28801 tgcagctttg caagcctgtc gacatgacgt gccgccgcta ggatctgatg cccttgctgg
    28861 ctgagtgtgc gcaccagatg ctgaccaatg tagccactgg caccgagaac taaaatgcgt
    28921 tgcggcacgt ctctctcctt aacgcgccag gaatgcgcgc cagtgggcgg cgacttccgc
    28981 cagttgttcg cgcgagacgt caagatgcgt caccaggcgg acaatcggcg aggcgttaat
    29041 cagcacgttt ctcgctttca tgtattcgcc taacgcggca gcattttctt ccccgacgcg
    29101 aacaaacagc atattagtgt cctgacgcat cacatccgcg cctgcttcac gcagttgctc
    29161 cgccatccag gcggcgttgt cgtgatcttc ctgcaaccgt gcgacgttat tttccagggc
    29221 atacatcccg gcggcagcca gaatcccgga ctgacgcatc ccgccaccgg ccattttccg
    29281 ccagcggatg gcacgtttaa tgtaatcacg attaccgacg agtaatgaac cgactggcgt
    29341 cccaagacct ttcgacaggc aaatggtgaa cgaatcacaa tattgcgtga tctctttcag
    29401 ttcgcagccg taagccacca cggcattaaa gatgcgcgca ccgtcaacat gcagcgccag
    29461 attgcgctcg cgggtaaatt cccatgcttc tttcaggtat tcacgcggca gcactttgcc
    29521 gttgtgggtg ttttccagac tgagtaattt ggtgcgggcg aaatggatat cgtcgggttt
    29581 gattttcatc gccactttat ccagcggtag cgtgccgtcg gcagccgcgt cgatgggttg
    29641 cggctgaata ctgcccagca ccgctgcgcc accggcttca aacagatagt tatgcgcggc
    29701 ctgaccgaca atatactctt cgccacgttc gcagtgactg agcagagcga ccaggttggc
    29761 ctgagtgccg gtaggcagaa aaatggcggc ttctttaccg gaaagctctg ctgcatagtc
    29821 ctgcagagca ttaacggtag ggtcgtctcc gtaaacgtcg tccccaaccg gggcggccat
    29881 catcgcttcg agcatggcgc ggctcggtcg ggtaacggta tcactgcgta aatcaatcat
    29941 ggcatgtcct tattatgacg ggaaatgcca ccctttttac cttagccagt tcgttttcgc
    30001 cagttcgatc acttcatcgc cgcgtccgct gataattgcg cgcaacatat acaggctaaa
    30061 acctttggcc tgttcgagtt tgatctgcgg tggaatggct aattcttctt tggcgaccac
    30121 cacatccacc aacaccggac cgtcgataga gaaggcgcgt tgcagggctt catcaacttc
    30181 agaggctttt tctacacgga tacccgtaat gccgcacgct tcggcaatgc gggcaaagtt
    30241 ggtgtcgtgc agctcagtac cgtctgtcag gtaacctccg gccttcatct ccatcgccac
    30301 aaagcccagc acgctgttat taaagacgat aattttcacc ggcagtttca tctgcactac
    30361 tgagaggaaa tcgcccatca acatgctaaa accgccatcg ccgcacatgg cgaccacctg
    30421 acgttccggc tcggttgcct gcgcacccaa cgcctgcggc atggcgttag ccatcgaacc
    30481 gtggttaaac gaacctaaca ggcgacgctt gccgttcatt ttcagataac gtgccgccca
    30541 taccgttggc gtgccgacgt cacaggtaaa gatggcgtca tcggcggcaa aatgactaat
    30601 ttgctgcgcc agatattgcg ggtgaatggc tttctcgctc ggtttagcta aatcgtccag
    30661 ccctttgcgg gcatcgcggt aatcttccag cgctttatcc agaaacttgc gatcggtttt
    30721 ttcttccacc agtggcagca atgcgcgcag ggttgatttg atatcgccga ccagcgccat
    30781 atctaccttg ctatgcgcgc cgatgctggc tggattaata tcaatctgaa taattttggc
    30841 atcggtcggg tagaacgcac ggtaggggaa ctgagtaccg agtagcacta acgtgtcggc
    30901 gttcatcatg gtatggaaac ctgacgagaa tccgattaac ccggtcattc caacatcata
    30961 cggattatcg tattcgacat gctctttacc gcgtagggca tgaacaatag gtgctttaat
    31021 tttcccggca aactcaacta actctttatg cgcccccgcg cagccgctgc cacacatcag
    31081 ggcgatattg ctggaataac gcagcagttg cgccagtttg cgtaactctt cttcttccgg
    31141 catcacgact ggctgtggcg catgatacca gtgcgtggtt gccccttctg gcgcaggttt
    31201 taacgccacg tcgcccggta atacaacaac ggaaacgcca cggttaagca ccgctttgcg
    31261 catagcaatt gccagcactt gtgggatctg ctccgggctg gaaaccagct cgcaatagtg
    31321 actacattcg cggaatagct cttgtgggtg ggtttcctgg aaatagccgc tgccaatttc
    31381 gctggaggga atatgagcgg caatcgccag taccggaacg ttattgcggt ggcaatcgaa
    31441 taggccgttg atcaagtgca ggttgcctgg gccgcacgat ccggcacaga ccgccagttc
    31501 tcctgaaagt tgtgcttcag cgccagcggc aaacgccgcc acttcttcat ggcgggtgga
    31561 catccactcg atggtgccca tgcgattaag actgtcgcta agaccgttca gagagtcgcc
    31621 cgtgactccc cagatgcgtt tcacgcctgc cgattcgagt gttttggcga tataagctgc
    31681 aaccgtttgt ttcatggttc tccatctcct gaatgtgata acggtaacaa gtttagttca
    31741 tctgacggag ggggaaggga tgggagagaa aggaggcact aacggttaaa tagcccgatg
    31801 aaaggaatat catcgggcat aaggcgatta tgcgagaacc aaatccccct gcggatggca
    31861 ggagcaggcc agtacgtaac cttcagcgat ttcggcgtcg gtcagcgtca ttgtactgct
    31921 caccgtatat tcaccggaaa ccacttttgt cttacagcag ccgcaaacac ccgcacggca
    31981 ggcagcgaca accggaacgt tattgctttc cagcgcctcc agtagcgtgg tgccaaccgg
    32041 ggcgtaaaat tctcgtgccg gttgcagttt ggtgaatttc agaccgctgg tcgccgcttc
    32101 tgctactggg gtgaagaatt tctctttaaa gaaacgcgtc acgccgagcg ctttcacttc
    32161 ctgctctacc caatccatat acggagccgg gccgcaggtc atcacggtac gtgaagctaa
    32221 gtcaggtaca tctgccagca gttcgcgagt gaggcgacca gcgataaagc cttcggtaac
    32281 gttattttct gccaccagcg ttaccggata gttacgccac tcatcggcga aaataacatc
    32341 ctgcggcgta cgcacgttgt agatcacccg cacatcggcc tgtggacggt tcttcgcaag
    32401 ccagcgacgc atcgacataa tcggcgtgac gccgcagcct gccgccagca acaggaattt
    32461 atcttctgct ttatcgtcgc aggtaaattc ccccatcgcg tccgaaagcc agagataatc
    32521 accgcgtttt acatcgcgcg tcagccactg ggagccgaca ccgtcatcaa tccgccgcac
    32581 ggtcagggtg atatattcac tcacgcctgg cgtggaggaa atggtgtaag cacgcagcgt
    32641 ttccgctgag tttcgcacgc tgaccagtgc atattgcccg gcgcgatatg ggtagtgatc
    32701 gtggcaaatc agggaaatcg tccacacatc cggcgtttct tgcgtaatgt gatgaacctg
    32761 tatccgccac gggcattgat tcgttggcat cgtcatcgac aaactcctta cgcgttcaac
    32821 agttgcttca tgtcttcttc aacagtggtg atagaacgca ggccgaattt ctcgttcagc
    32881 accgccagca ggtctggtgt caggaaacca ggtgcagtcg ggccggtgac gatatttttc
    32941 acgcccagag aaagcagcgt cagcagaatg acaatcgctt tctgttcaaa ccaggagagc
    33001 accagcgaca gcggcagatc gttcacacca cagcccagtt tctctgccag agtgacagcc
    33061 agaataattg ctgagtaagc atcgttacat tgacctgcat ctaccagacg cggcagacct
    33121 tcgatatcgc caaactcaag tttgttaaag cgatatttac cacaggcgag ggtcaggatc
    33181 aggcagtcat ccggcacgct ggtggcgaaa tcggtgaagt agtggcgctc gccgcgtgcg
    33241 ccgtcacagc caccaagcag gaagatatga cgcagttttt cacggctaac cagatcaatc
    33301 agcgtatcgg ctgcaccaag cagcgtctgg cgaccaaagc ccacggtgat caggtgagga
    33361 atttcgctgt acgggaagcc cgccatttgt tgcgcctggg cgatgaccgc agagaaatct
    33421 tcgccatcca gatgacgcac gccaggccag ccaacgatgc tgcgggtcca gatacgatcg
    33481 tcataagcgc ctacggttgg gtcgatgatg cagttcgagg tcatcacgat ggggccaggg
    33541 aaacgagcga actccacttg ctgattctgc cagccgctgc cgtagttacc gaccagatgc
    33601 ttgaatttac gcagctccgg gtagccatgc gcaggcagca tttcgccgtg ggtgtagaca
    33661 ttaacgcccg tgccttcggt ctgttccagc aggttgtaga gatctttgag atcgtgaccg
    33721 gaaatcagaa tgcatttacc cgccgtcgct ttgacgttga cctgggttgg cgtcgggtga
    33781 ccgtatttac cggtttcgcc tgcatccaga atgctcatca ctttgaagtt catctggccg
    33841 atttccattg aacactcaag aagcgcgttc atatcggcag gccaggtccc cagccacgcc
    33901 atgattttat ggtactgggc ataaatatcg ttgtcgtatt gaccgagaac atgcgcgtgt
    33961 tccatatagg ccgccgcacc tttcaggcca tacaggcaca gcagacgcag gccgagaatg
    34021 ttttcgccaa tcgccgcttt atctttgtta ggggtaaatt ctgctgcctg acgttgcagc
    34081 tcgccgagat catcgctcac cagttgcagg tcagccatcg ggttatcgac gcgcgcgttg
    34141 gcatctacag ccaggcattg tgctttcagc gcctcgcgca aggcaatcgc ttcacgagcg
    34201 tagccgacaa tacgcggaga atcgaagtta acgttggtca gggttgagaa aaaggcacgt
    34261 ggcgcgaagc tgtcaacatc gtggttgatg atgccgtatt cacgcgcttt taccgcccag
    34321 gcagaaagcc cttgcagcgc cgcgatgagt aaatcctgaa ggtcagaagt ttccgccgtt
    34381 ttaccacaca tcccctgcgc gtatgagcag ccgttttctg ccggagtacg gatagtttgt
    34441 tcacattgca cacaaaacat gatcacacct tttaaagtta tatttaatat acatgtttaa
    34501 gggtaagacg cttaacgcgg ggataaaagg gatttttcat gcaactttaa gggagattga
    34561 tttagcgcaa ttttggcggc agggaactac cgccagagag gtattacgca gagaaaaagg
    34621 cgatgaggat cggcactaac aggctaagaa taaaaccgtg aacaattgcc gccgggacca
    34681 tatccagccc gccagtacgt tgaagaacgg gcagggtgaa atccattgat gtggcaccgc
    34741 ataagcccag tgcagtagag cggctgcggc gaatcagccc agggatcaac ataatagcaa
    34801 tcagttcacg ggccagatca ttaaaaaacg ccgcgctccc gattaccgga ccaaaagatt
    34861 cggtcaataa aataccggaa agagaatacc agccgaaacc ggaggccatt gccagcgcgg
    34921 tattgatggg gagatcaaga ataaaggcgt taattaaacc accaattaat gaactgacaa
    34981 ccaccaccac ggcgacaatc attccccggc gattaaggac aatctgcttt aaggtcatgc
    35041 cattattgcg caactgaata ccaacgagga aaagtagcaa aattaacgtg tattcactgg
    35101 cttcggtcgc gtgttgtaag aaagccagtc cactcagacc aatggcaaaa ccaatcacta
    35161 ctacgccgca cagttttagc gactccagcg ccatcgcaat acgcgacggg agtttttctt
    35221 gctgatggtg gttgcgccac ggcaggcctc gctccagcca catcagggcg gcaatattac
    35281 acagtaaaat aacggtaata ctgacggcag aataatgcag aatcgccaac aggttactgg
    35341 cgaggttatc gagaaacgcc agactgatac ccataaaaaa gagaataagg taaaccatcc
    35401 agcttaatag ctgattaata gcttttaacg cagcttgttg gcgaagcgga atgaggtaac
    35461 ccacaatcag gggaaccaga atgattaaca gcccagaaaa catgaaaatc cagtccttgc
    35521 aaagaagaag tcgaaatgcg cgatgacaca ctactgaaag cggaagggcg agtaaagttg
    35581 caattaaaag gaaatgttat gcatcagag
//
LOCUS       DAJTPG010000032        35124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTPG010000032 DAJTPG010000000
VERSION     DAJTPG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..783)
                     /locus_tag="OO230_001671"
     CDS             complement(<1..783)
                     /locus_tag="OO230_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5691471.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="OO230_001672"
                     /pseudo
     CDS             875..961
                     /locus_tag="OO230_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1366..1842)
                     /locus_tag="OO230_001673"
     CDS             complement(1366..1842)
                     /locus_tag="OO230_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5691472.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OO230_001674"
     CDS             complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="OO230_001674"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5691473.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3277..4317
                     /gene="bioB"
                     /locus_tag="OO230_001675"
     CDS             3277..4317
                     /gene="bioB"
                     /locus_tag="OO230_001675"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5691474.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4314..5468
                     /gene="bioF"
                     /locus_tag="OO230_001676"
     CDS             4314..5468
                     /gene="bioF"
                     /locus_tag="OO230_001676"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5691475.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5455..6210
                     /gene="bioC"
                     /locus_tag="OO230_001677"
     CDS             5455..6210
                     /gene="bioC"
                     /locus_tag="OO230_001677"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5691476.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6203..6880
                     /gene="bioD"
                     /locus_tag="OO230_001678"
     CDS             6203..6880
                     /gene="bioD"
                     /locus_tag="OO230_001678"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5691477.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7459..9480
                     /gene="uvrB"
                     /locus_tag="OO230_001679"
     CDS             7459..9480
                     /gene="uvrB"
                     /locus_tag="OO230_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5691478.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OO230_001680"
     CDS             complement(9672..10580)
                     /gene="yvcK"
                     /locus_tag="OO230_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5691479.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10845..10989
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10977..11966
                     /gene="moaA"
                     /locus_tag="OO230_001681"
     CDS             10977..11966
                     /gene="moaA"
                     /locus_tag="OO230_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5691480.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11988..12500
                     /gene="moaB"
                     /locus_tag="OO230_001682"
     CDS             11988..12500
                     /gene="moaB"
                     /locus_tag="OO230_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5691481.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12503..12988
                     /gene="moaC"
                     /locus_tag="OO230_001683"
     CDS             12503..12988
                     /gene="moaC"
                     /locus_tag="OO230_001683"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5691482.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12981..13226
                     /gene="moaD"
                     /locus_tag="OO230_001684"
     CDS             12981..13226
                     /gene="moaD"
                     /locus_tag="OO230_001684"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5691483.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13228..13680
                     /gene="moaE"
                     /locus_tag="OO230_001685"
     CDS             13228..13680
                     /gene="moaE"
                     /locus_tag="OO230_001685"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5691484.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13817..14521
                     /locus_tag="OO230_001686"
     CDS             13817..14521
                     /locus_tag="OO230_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5691485.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14726..15439
                     /locus_tag="OO230_001687"
     CDS             14726..15439
                     /locus_tag="OO230_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5691486.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15475..16431)
                     /locus_tag="OO230_001688"
     CDS             complement(15475..16431)
                     /locus_tag="OO230_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5691487.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OO230_001689"
     CDS             complement(16431..17672)
                     /gene="clsB"
                     /locus_tag="OO230_001689"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5691488.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17669..18430)
                     /locus_tag="OO230_001690"
     CDS             complement(17669..18430)
                     /locus_tag="OO230_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5691489.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18563..18973
                     /locus_tag="OO230_001691"
     CDS             18563..18973
                     /locus_tag="OO230_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5691490.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18935..20041)
                     /locus_tag="OO230_001692"
     CDS             complement(18935..20041)
                     /locus_tag="OO230_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5691491.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20052..21185)
                     /locus_tag="OO230_001693"
     CDS             complement(20052..21185)
                     /locus_tag="OO230_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5691492.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21178..22914)
                     /locus_tag="OO230_001694"
     CDS             complement(21178..22914)
                     /locus_tag="OO230_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5691493.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OO230_001695"
     CDS             complement(22907..23902)
                     /gene="hlyD"
                     /locus_tag="OO230_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5691494.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OO230_001696"
     CDS             complement(23905..24576)
                     /gene="cecR"
                     /locus_tag="OO230_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5691495.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24805..25992
                     /gene="rhlE"
                     /locus_tag="OO230_001697"
                     /pseudo
     CDS             24805..25992
                     /gene="rhlE"
                     /locus_tag="OO230_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OO230_001698"
     CDS             complement(26199..26681)
                     /gene="ybiA"
                     /locus_tag="OO230_001698"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5691496.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26801..28951
                     /gene="dinG"
                     /locus_tag="OO230_001699"
     CDS             26801..28951
                     /gene="dinG"
                     /locus_tag="OO230_001699"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5691497.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28979..29941
                     /gene="ybiB"
                     /locus_tag="OO230_001700"
     CDS             28979..29941
                     /gene="ybiB"
                     /locus_tag="OO230_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5691498.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30082..31167
                     /gene="hcxB"
                     /locus_tag="OO230_001701"
     CDS             30082..31167
                     /gene="hcxB"
                     /locus_tag="OO230_001701"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5691499.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31396..31656)
                     /locus_tag="OO230_001702"
     CDS             complement(31396..31656)
                     /locus_tag="OO230_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5691500.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="OO230_001703"
     CDS             complement(31921..32187)
                     /gene="ybiI"
                     /locus_tag="OO230_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5691501.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="OO230_001704"
     CDS             complement(32261..32938)
                     /gene="ybiX"
                     /locus_tag="OO230_001704"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5691502.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32980..34989)
                     /locus_tag="OO230_001705"
                     /pseudo
     CDS             complement(32980..34989)
                     /locus_tag="OO230_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8319 a         9005 c         9483 g         8317 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 tctaagtggt tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat
     1201 tttttaagct aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca
     1261 gcttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat
     1321 acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc
     1381 ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct
     1441 ggctccttca tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg
     1501 atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg
     1561 cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac
     1621 gcgggtatca gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc
     1681 atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc
     1741 cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg
     1801 caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt
     1861 tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt
     1921 catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa
     1981 tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga
     2041 caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg
     2101 cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc
     2161 gcagctgcac ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta
     2221 ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa
     2281 agcagccggc ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg
     2341 aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt
     2401 ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg
     2461 caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat
     2521 ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt
     2581 catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac
     2641 catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca
     2701 ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat
     2761 aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca
     2821 acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact
     2881 ccagcggttg cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg
     2941 tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa
     3001 gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc
     3061 gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca
     3121 gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt
     3181 ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc
     3241 gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca
     3301 ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag
     3361 caggtgcatc gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt
     3421 aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc
     3481 gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg
     3541 aaagcggcag gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc
     3601 gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt
     3661 atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat
     3721 tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc
     3781 acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc
     3841 tcaggtggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa
     3901 ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa
     3961 ggcacgccgc ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc
     4021 gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg
     4081 aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc
     4141 aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg
     4201 gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt
     4261 ggacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct
     4321 ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc
     4381 gttatccggt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga
     4441 acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga
     4501 agcagggggc ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata
     4561 gcgtggtgca tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc
     4621 tgctgtttat ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag
     4681 aggaccgtat tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa
     4741 gcccgtcgca gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg
     4801 cttccccctg tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg
     4861 atagcgcgcc actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg
     4921 tcgatgatgc ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc
     4981 aaaaggtaaa accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg
     5041 ctgcggtgct ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta
     5101 tctacagcac cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca
     5161 ttcgccgtga tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc
     5221 gtgccggagt acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat
     5281 tgatcgtcgg tgataacagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct
     5341 gctgggtcac ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa
     5401 cgctaaccgc tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca
     5461 acggttaata aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa
     5521 catgcagatc tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa
     5581 tacacccacg tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa
     5641 cgtcacgcgc aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat
     5701 aaagatgccg cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg
     5761 acgttcgatc ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca
     5821 ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg
     5881 cagggatcgt tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct
     5941 aatcgctttt taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat
     6001 catattcagc tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa
     6061 ggcatcggtg ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag
     6121 ttgcagcgat tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat
     6181 ctttttttgg gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga
     6241 agtggggaaa accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg
     6301 gacggcaggt tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa
     6361 tagtgacgcg ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa
     6421 tccttacacc ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag agggcaggcc
     6481 gatagaatca ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt
     6541 gttagtggaa ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga
     6601 ttgggtaaca caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat
     6661 taatcacgcg atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg
     6721 ggtggcgaac gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac
     6781 ccgcatgatt cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa
     6841 tgcggcaacc ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa
     6901 ttgcgagcga gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat
     6961 cacgaaaaaa attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg
     7021 ggataggggc tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta
     7081 gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc
     7141 tcaaagaaat atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca
     7201 ggcatctctt gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc
     7261 tttggggata acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg
     7321 attgacagcg gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc
     7381 agtataattt gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac
     7441 tccttcaggt agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct
     7501 ggcgatcagc cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac
     7561 cagacgttac ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct
     7621 gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat
     7681 ggcgaaatga aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac
     7741 tactatcagc cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg
     7801 gttaacgaac atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt
     7861 gatgtgattg tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat
     7921 ctcaagatga tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc
     7981 cgactggcgg agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc
     8041 gttcgtggcg aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg
     8101 gaactgtttg acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt
     8161 gtttccacta ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag
     8221 cgcatcgtac aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg
     8281 ttggaaaaca acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg
     8341 gagatgatgg acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc
     8401 ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg
     8461 ctggtcgtcg atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac
     8521 cgggcgcgta aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac
     8581 cgtccgctta agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg
     8641 acgccgggta attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt
     8701 ccaaccggat tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat
     8761 cttctttcgg agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg
     8821 accaagcgaa tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt
     8881 tatcttcact cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg
     8941 ggtgagttcg acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa
     9001 gtgtcgctgg tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg
     9061 ttgatccaga ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc
     9121 gataggagca ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa
     9181 cagcagaagt acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc
     9241 gatatcctgg cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg
     9301 cgcccgattg ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag
     9361 aaaatccatg aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa
     9421 gcggcacaaa ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa
     9481 caggatagcg aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag
     9541 gcctacattt tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc
     9601 acgccgcatc cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct
     9661 tcggatttcc gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg
     9721 cggtcatgac gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct
     9781 ttcaccgccg agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca
     9841 acatactgct ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc
     9901 aactcacgcc ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg
     9961 gcgatttcct tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata
    10021 atgatgagat ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc
    10081 gttaacaata actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact
    10141 tcatgcccct gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa
    10201 tgcgtatcca ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta
    10261 aggtgatcca gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg
    10321 ccgccaaaac ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg
    10381 aggcagttgc gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc
    10441 gagccaccat tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt
    10501 gatgagagaa cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca
    10561 gccagcgtac gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc
    10621 aaattgcggg gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta
    10681 tctctaaagg acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca
    10741 ttttccctta ttctgtagcg aaatagcacg atcatgacgc tatatatatg attacatagc
    10801 gaaagtgtgg atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact
    10861 ctagcctctg cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga
    10921 ttgccaccag ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg
    10981 cttcacaact gactgatgta tttgcgcgta agttttacta cttgcgcctg tcgattaccg
    11041 atgtgtgtaa ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca
    11101 ccaataaagg ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc
    11161 tgggcaccga aaaagtgcgc ctgacaggag gagagccgtc tttacgccgc gactttaccg
    11221 atatcatcgc cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg
    11281 gttaccgtct ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg
    11341 tcagtgtcga cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca
    11401 accaggtcat ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca
    11461 ataccgtgct gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc
    11521 agcatcgccc tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc
    11581 tcttccgtaa gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct
    11641 ggatccacca attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt
    11701 acgccggaga gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc
    11761 gcctgcgcgt ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta
    11821 acctgcgcga tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag
    11881 cggcgctgcg ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc
    11941 aaaacttatc gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa
    12001 gcactgaatt tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag
    12061 aagacgatac ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg
    12121 tcgatgaagc cattgtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga
    12181 tcgccagcga cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg
    12241 atcaggctcc cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag
    12301 tgttccgtat gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag
    12361 cgggcgtcgc taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta
    12421 ccgcatggga aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc
    12481 atccacattt gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc
    12541 gcacatggtg gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt
    12601 tgtcaccatg cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga
    12661 cgtatttgcc actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat
    12721 cccgctgtgt cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga
    12781 gcacaatcgg gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat
    12841 ggaagcgtta accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt
    12901 gcaaaaagat atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg
    12961 tgactttaag gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg
    13021 tgggaacaga tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc
    13081 acctggctgc gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg
    13141 ccgtcaacca gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt
    13201 tcttcccgcc ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag
    13261 ccgttcagcg taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta
    13321 gtcaccttca ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc
    13381 ctcgaacact atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt
    13441 aaccgctggc cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc
    13501 gatgaaatcg tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg
    13561 cagtttatta tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg
    13621 gaaggcgacc gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag
    13681 ttttttgtta gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg
    13741 tttacgctgc gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata
    13801 tcttcaggag atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccctgggc
    13861 tggcttgcaa acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac
    13921 cgcatttgtt gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa
    13981 ccgtgtcttt ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc
    14041 gatgattcaa aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct
    14101 gacgggtctt acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac
    14161 tttcgtcgtt actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg
    14221 cgatttaagt ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc
    14281 gctggtcaac ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt
    14341 gattgtcttt gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca
    14401 gattgatacc cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt
    14461 gtatctggac ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta
    14521 attctttttc accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga
    14581 cacttaacaa gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat
    14641 ccataaatac aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat
    14701 gttttttcta aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga
    14761 ttttcagcac gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt
    14821 ggcaaccgca ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga
    14881 ttcaccacca atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa
    14941 ggatatcaac aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt
    15001 ggtggcgatt gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac
    15061 tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg
    15121 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa
    15181 tgcggcatta atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg
    15241 gccaggcatt atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag
    15301 tgaagagttg tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta
    15361 ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca
    15421 tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat
    15481 cgccgcttca tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat
    15541 cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat
    15601 aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac
    15661 accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc
    15721 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac
    15781 catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac
    15841 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
    15901 aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata
    15961 ccagtaatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata
    16021 gcccagccag ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa
    16081 ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt
    16141 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
    16201 accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac
    16261 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
    16321 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
    16381 ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt
    16441 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga
    16501 tcaacctgcg tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg
    16561 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc
    16621 atggtttcat ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc
    16681 gtctggttaa aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg
    16741 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
    16801 ccgtggagcg ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta
    16861 tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
    16921 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg
    16981 aagaagtagg cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa
    17041 tggcgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
    17101 tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg
    17161 ctctgtccag gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca
    17221 aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag
    17281 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
    17341 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac
    17401 actacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
    17461 ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc
    17521 agctgtttgc cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt
    17581 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
    17641 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
    17701 gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
    17761 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
    17821 ggcgtccgtg ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat
    17881 tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta
    17941 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
    18001 ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt
    18061 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa
    18121 tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac
    18181 cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
    18241 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
    18301 tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa
    18361 acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt
    18421 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
    18481 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga
    18541 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct
    18601 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
    18661 gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt
    18721 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
    18781 ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc
    18841 ccgcgtactg gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc
    18901 ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
    18961 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
    19021 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
    19081 tagggttaat ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt
    19141 aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt
    19201 gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
    19261 ccatcgtaaa gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac
    19321 caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc
    19381 cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt
    19441 cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg
    19501 cgatcagcga cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta
    19561 ccaccagctc gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt
    19621 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
    19681 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
    19741 aacgcaccag tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt
    19801 tcagcagcag cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg
    19861 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg
    19921 cggcgaacgg gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt
    19981 gtggttcgcg cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
    20041 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
    20101 accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg
    20161 aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg
    20221 cggatcaccg cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc
    20281 agaaaagcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa
    20341 atcagcagcc ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
    20401 agcagcgaac cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac
    20461 atcgccagca tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg
    20521 gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc
    20581 agcgaggtga gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata
    20641 aagtgctggc ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt
    20701 tcaaaagtct gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg
    20761 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
    20821 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga
    20881 ccgcgaattt tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg
    20941 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
    21001 tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa
    21061 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg
    21121 atctgccgcg tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta
    21181 ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt
    21241 gggatcgggt tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc
    21301 gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
    21361 gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa
    21421 ttcacggcgg gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg
    21481 ttcatgcatc agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc
    21541 ggtggcgtgt gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc
    21601 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
    21661 cgtcaggttg ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt
    21721 accggaactc tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag
    21781 caaaccgcac atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc
    21841 aaaaatctcc ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
    21901 tttcttggtc agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
    21961 caatattgca cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc
    22021 atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg
    22081 aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat
    22141 gccatcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg
    22201 tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc
    22261 ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
    22321 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
    22381 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
    22441 tttcggttcg cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag
    22501 cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa
    22561 agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
    22621 catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
    22681 cagcgcgccg tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg
    22741 tttcagtaat cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac
    22801 caaccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc
    22861 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
    22921 cctcgtcacc gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg
    22981 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
    23041 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg
    23101 ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt
    23161 catcaacata agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac
    23221 cttcattgag aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg
    23281 ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt
    23341 cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca
    23401 atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt
    23461 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
    23521 tctgcgcata gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt
    23581 cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg
    23641 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
    23701 ccgctttgat agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga
    23761 aactaagatt caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
    23821 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc
    23881 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
    23941 gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
    24001 ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat
    24061 caacgcatgg gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc
    24121 aatcaggcgt gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta
    24181 ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc
    24241 ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc
    24301 ccgatcaggc tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg
    24361 ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
    24421 gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt
    24481 ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc
    24541 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
    24601 gattgattaa gattatgact ccatatggga gttgtccagt atggctaaga attttagcta
    24661 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
    24721 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
    24781 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
    24841 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
    24901 cctgctgtgc tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca
    24961 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    25021 cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc
    25081 gaaaacgtcc gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt
    25141 gtcagcatta acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg
    25201 ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    25261 ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    25321 gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac
    25381 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    25441 cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    25501 cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc
    25561 cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt
    25621 gtggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa
    25681 tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa
    25741 gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt
    25801 atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat
    25861 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    25921 attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    25981 caacgtggcg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    26041 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    26101 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    26161 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    26221 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    26281 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    26341 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    26401 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    26461 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    26521 tgtgcctgaa aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg
    26581 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    26641 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    26701 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    26761 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct
    26821 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    26881 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    26941 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    27001 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    27061 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    27121 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct
    27181 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    27241 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    27301 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    27361 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    27421 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    27481 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    27541 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    27601 caccgtgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    27661 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    27721 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    27781 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    27841 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    27901 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    27961 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    28021 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    28081 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    28141 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    28201 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    28261 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    28321 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    28381 acaggtggag agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat
    28441 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    28501 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    28561 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    28621 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    28681 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    28741 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    28801 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    28861 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa
    28921 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    28981 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    29041 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    29101 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    29161 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    29221 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    29281 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    29341 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    29401 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    29461 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    29521 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag
    29581 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    29641 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    29701 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    29761 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    29821 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    29881 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta
    29941 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    30001 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    30061 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    30121 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    30181 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    30241 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    30301 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    30361 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    30421 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    30481 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    30541 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    30601 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    30661 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    30721 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    30781 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    30841 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    30901 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    30961 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    31021 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    31081 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    31141 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    31201 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    31261 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    31321 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    31381 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    31441 ggcggaagtg atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc
    31501 ttccagggca tcgagggtgg atgcgccatc ggcagaaacc acgccgattt tattcatgtt
    31561 ctgtgcctgg gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag
    31621 agccatagca gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt
    31681 tgtataagtg acgttgtttc gtcgatgtga tgagtatcac gtttttttac gagagataaa
    31741 atcgaagaga attgaccgcc ttgttcaaat aaattgattg atatctaaca tactgataaa
    31801 ccatttattt gttgtactta tggacttgtt ttatgccgcg tggcagtcac agcagcgtta
    31861 aaggcacgga atgccacgca aaatacaaat tatactcgct atgcatctcg ccagttgtag
    31921 ttaacgtaac tgtctgtctt tcgaacctct gcgattatat cctgtatacg ctggtttttg
    31981 taaatctttc tcctgctgac aatgaataca taagcgcacg ccaggaatgg cttcccgacg
    32041 ggcttgcggg atgggggcac cgcactcttc acattcatac aggctttcgc cacgcggaat
    32101 ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt cgttgacggc
    32161 gtcatcgtta gcccaagcgg atgccatatc gtcctcccca tatcaatgct tgtacagtta
    32221 agtgtagcta atatagggac gaacctgggc agttcaagcg tcagatctcc gaccattccc
    32281 gcagcagatt atgataaaga ttgagcagcg acaggatctc ttcattttca ccatagcggc
    32341 ttttcagcga ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc
    32401 ggatcatcga ctggatccac ataaatgatg ccactcgtac gccgcgggtt acgggtgtca
    32461 cgcaatgcag gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat
    32521 gttgtccaaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta
    32581 aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg
    32641 cgccatccac atggaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg
    32701 aaagggtacg cggcaaggca gcggcaaaga acaatgcatg ttggttaacc gcgttcagca
    32761 cctcattttg cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga ttgttcttaa
    32821 cttgtgcacc ttgtgctccg gtggtgacgc gtccatccac ccattcggct tgttccagtt
    32881 gttcgcgaaa atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac
    32941 aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt
    33001 gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttgt tgattgaggc
    33061 gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat tgcgattaac
    33121 ccgatacccc agtttggcat cggcgaccca gtaaccttct gtaaatgctg gcgttcccac
    33181 cgcgccgtct gaacctttat gtatactgcc gatatagcgt gcgcccgcgc caacagagat
    33241 atcatcggtt gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag
    33301 cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt
    33361 atagccgcca atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc
    33421 ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt
    33481 ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca
    33541 tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact
    33601 gttaccactg ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac
    33661 ggcatagtta atatagacat tgccgttttc cgtcaggtgg tacagcgccc cggctttcca
    33721 gttcaccaga ttgcccgact tggcggtgtc gacggtggtg actggagaac cttttgccac
    33781 accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata
    33841 ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg
    33901 cagcgtatcg aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt
    33961 gcgcgtcagg ccgccgggat gaattctgct gtcaggatga taaatattta ccgccggtaa
    34021 cgtcaccgga ttaacgccat agttagtctg cgtttcacgg gtaaattcca cgccggtact
    34081 gacatcatga ccgatagaac cggtatagaa cgtcgaggtc aggttggtct ggttggtgag
    34141 aattttatta ctcacatctt tggtattcgc cgtgcgtgac taggtccagc tattcacatc
    34201 gctggtgggc tgagtaatat tcgacgcccc gcccataatc gccgtcatca ggtaatcctg
    34261 ctttacgcgc gaccaacggg tagtattgcg aatggtggtg ttatcgttga tgtcgtgctc
    34321 aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa
    34381 gttatgagta tcaacttttc cggaatgatt cagggccgcc gctcccgcag atggggcaga
    34441 atagcccggc aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct gagtgacatg
    34501 cagataatta agatacaaac gatttgctgt accaaggcca aaagcgacag aaggggcgac
    34561 gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgggtttttt cgcccatcac
    34621 attcaggcgc accgcggtag tatcaccaat gacctgattg acgtccagcg tgccgcggcg
    34681 gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc gcggctgctt
    34741 gctgatcata ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc cggacggccc
    34801 tttaatcact tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg
    34861 aataccatca atataaatac tgttagaggt atcggcacca cgcatataaa tggcgtcgcc
    34921 agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgccggggt aatgccgatc
    34981 agttaaggat cagttgaccg atccagtggc tgtgtaagaa tccggaaacg ctcacttgtt
    35041 tccggatttt tttatgcaca ttggacaggc tcttgatctg gtatcccgtt acgattctct
    35101 gcgtaaccca ctgacttctc tggg
//
LOCUS       DAJTPG010000033        34878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTPG010000033 DAJTPG010000000
VERSION     DAJTPG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            833..3118
                     /gene="nrdA"
                     /locus_tag="OO230_001706"
     CDS             833..3118
                     /gene="nrdA"
                     /locus_tag="OO230_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5691503.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3352..4482
                     /gene="nrdB"
                     /locus_tag="OO230_001707"
     CDS             3352..4482
                     /gene="nrdB"
                     /locus_tag="OO230_001707"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5691504.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4482..4736
                     /gene="yfaE"
                     /locus_tag="OO230_001708"
     CDS             4482..4736
                     /gene="yfaE"
                     /locus_tag="OO230_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5691505.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4790..5440)
                     /gene="inaA"
                     /locus_tag="OO230_001709"
     CDS             complement(4790..5440)
                     /gene="inaA"
                     /locus_tag="OO230_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5691506.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5619..5861
                     /locus_tag="OO230_001710"
                     /pseudo
     CDS             5619..5861
                     /locus_tag="OO230_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5903..6979)
                     /gene="glpQ"
                     /locus_tag="OO230_001711"
     CDS             complement(5903..6979)
                     /gene="glpQ"
                     /locus_tag="OO230_001711"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5691507.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6984..8342)
                     /gene="glpT"
                     /locus_tag="OO230_001712"
     CDS             complement(6984..8342)
                     /gene="glpT"
                     /locus_tag="OO230_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5691508.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8615..10243
                     /gene="glpA"
                     /locus_tag="OO230_001713"
     CDS             8615..10243
                     /gene="glpA"
                     /locus_tag="OO230_001713"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5691509.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10233..11492
                     /gene="glpB"
                     /locus_tag="OO230_001714"
     CDS             10233..11492
                     /gene="glpB"
                     /locus_tag="OO230_001714"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5691510.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11489..12679
                     /gene="glpC"
                     /locus_tag="OO230_001715"
     CDS             11489..12679
                     /gene="glpC"
                     /locus_tag="OO230_001715"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5691511.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12872..13822
                     /locus_tag="OO230_001716"
     CDS             12872..13822
                     /locus_tag="OO230_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5691512.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13863..14333)
                     /locus_tag="OO230_001717"
     CDS             complement(13863..14333)
                     /locus_tag="OO230_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5691513.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14388..15590)
                     /locus_tag="OO230_001718"
     CDS             complement(14388..15590)
                     /locus_tag="OO230_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5691514.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15677..16219)
                     /locus_tag="OO230_001719"
     CDS             complement(15677..16219)
                     /locus_tag="OO230_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691515.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16498..16923
                     /gene="nudI"
                     /locus_tag="OO230_001720"
     CDS             16498..16923
                     /gene="nudI"
                     /locus_tag="OO230_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5691516.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16962..17564)
                     /locus_tag="OO230_001721"
     CDS             complement(16962..17564)
                     /locus_tag="OO230_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5691517.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17872..19011
                     /gene="arnB"
                     /locus_tag="OO230_001722"
     CDS             17872..19011
                     /gene="arnB"
                     /locus_tag="OO230_001722"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5691518.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19015..19983
                     /gene="arnC"
                     /locus_tag="OO230_001723"
     CDS             19015..19983
                     /gene="arnC"
                     /locus_tag="OO230_001723"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5691519.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19983..21964
                     /gene="arnA"
                     /locus_tag="OO230_001724"
                     /pseudo
     CDS             19983..21964
                     /gene="arnA"
                     /locus_tag="OO230_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21961..22851
                     /locus_tag="OO230_001725"
     CDS             21961..22851
                     /locus_tag="OO230_001725"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5691520.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22851..24503
                     /gene="arnT"
                     /locus_tag="OO230_001726"
     CDS             22851..24503
                     /gene="arnT"
                     /locus_tag="OO230_001726"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5691521.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24500..24835
                     /gene="arnE"
                     /locus_tag="OO230_001727"
     CDS             24500..24835
                     /gene="arnE"
                     /locus_tag="OO230_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5691522.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24835..25221
                     /gene="arnF"
                     /locus_tag="OO230_001728"
     CDS             24835..25221
                     /gene="arnF"
                     /locus_tag="OO230_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5691523.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25215..25487)
                     /gene="pmrD"
                     /locus_tag="OO230_001729"
     CDS             complement(25215..25487)
                     /gene="pmrD"
                     /locus_tag="OO230_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5691524.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25592..26947)
                     /gene="menE"
                     /locus_tag="OO230_001730"
     CDS             complement(25592..26947)
                     /gene="menE"
                     /locus_tag="OO230_001730"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5691525.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26944..27906)
                     /gene="menC"
                     /locus_tag="OO230_001731"
     CDS             complement(26944..27906)
                     /gene="menC"
                     /locus_tag="OO230_001731"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5691526.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27906..28763)
                     /gene="menB"
                     /locus_tag="OO230_001732"
     CDS             complement(27906..28763)
                     /gene="menB"
                     /locus_tag="OO230_001732"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5691527.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28778..29536)
                     /gene="menH"
                     /locus_tag="OO230_001733"
     CDS             complement(28778..29536)
                     /gene="menH"
                     /locus_tag="OO230_001733"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5691528.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29533..31203)
                     /gene="menD"
                     /locus_tag="OO230_001734"
     CDS             complement(29533..31203)
                     /gene="menD"
                     /locus_tag="OO230_001734"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5691529.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31292..32587)
                     /gene="menF"
                     /locus_tag="OO230_001735"
     CDS             complement(31292..32587)
                     /gene="menF"
                     /locus_tag="OO230_001735"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5691530.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32666..32971)
                     /gene="elaB"
                     /locus_tag="OO230_001736"
     CDS             complement(32666..32971)
                     /gene="elaB"
                     /locus_tag="OO230_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5691531.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33026..33487)
                     /locus_tag="OO230_001737"
     CDS             complement(33026..33487)
                     /locus_tag="OO230_001737"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5691532.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33552..34469
                     /gene="rbn"
                     /locus_tag="OO230_001738"
     CDS             33552..34469
                     /gene="rbn"
                     /locus_tag="OO230_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5691533.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34660..34848
                     /locus_tag="OO230_001739"
                     /pseudo
     CDS             34660..34848
                     /locus_tag="OO230_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8531 a         9333 c         8641 g         8373 t
ORIGIN      
        1 ctggcgtgac aactcgcttt gatatcatat ccctggcata aatcaatggc cgccgcgaca
       61 ccgttagcag agaaaagaga tgcaatcatt gacgggagta gagataaata ctccttgcgt
      121 agaaagataa tccgcatatt aatctgacca tcattttata taagacggca tatttaaacg
      181 cattatacaa tatgggttat ctttcttttg taaattgtcc aacccgatgc aatggctatt
      241 ttgttattat ttgatgaata atatcagtgc gtcataattc aagttaataa ccttaaggga
      301 taccagttat atttaaacta aattaaagta atgaataatt ttcttataat ataaggtaaa
      361 ttaacaaaat ggcttcgcat ttaacaataa ccgaatagaa aacaaccatt tcgacatcaa
      421 caatcgctta cattcgctta tatattgacc acaactgata catcagatta tgtgatgact
      481 cgtgcttaga tcaatttttg caatcattag caaaaagatt aataaaccat ctatatcaat
      541 ttatccaacc tattctgccg ttcaagaaat cgccgaacag ttatttttaa caaatttttc
      601 tcttcccatt gactttcccg gacaccttgt ctgacctaag gtgcgcgaaa gccacttttt
      661 ccttcctgag ttatccacaa agttatgcac ttgcaagagg gtcattttca cactatcttg
      721 cagtgaatcc caaacatacc ccctatatat agtgttctaa gcagcttccc ttactacagg
      781 tagtctgcat gaaactattg cggaaagaat tccaaaaaca ggtacgacat acatgaatca
      841 gaatctgctg gtgacaaagc gcgacggtag cacagagcgc atcaatctcg acaaaatcca
      901 tcgcgttctg gattgggcgg cagaaggact gcataacgtt tcgatttccc aggtcgagct
      961 gcgctcccac attcagtttt atgacggtat caagacctct gacatccacg aaactattat
     1021 caaggctgcc gcagacctga tctcccgtga tgcgccggat tatcagtatc tcgccgcgcg
     1081 cctggcgatc ttccacctgc gtaaaaaagc ctacggccag tttgagccgc ctgcgctgta
     1141 cgaccacgtg gtgaaaatgg tcgagatggg caaatacgat aatcatctgc tggaagacta
     1201 cacggaagaa gagttcaagc agatggacac gtttatcgat cacgaccgtg atatgacctt
     1261 ctcttatgct gccgttaagc agctggaagg caaatatcta gtacagaacc gcgtgaccgg
     1321 cgaaatctac gaaagcgccc agttccttta tattcttgtt gccgcgtgcc tgttctcgaa
     1381 ctacccgcgt gaaacgcgcc tgcaatacgt gaagcgtttt tatgacgcgg tttccacatt
     1441 taaaatttcg ctgccgacgc caatcatgtc cggcgtgcgt accccgactc gtcagttcag
     1501 ctcctgcgta ctgatcgagt gcggtgacag cctggattcc atcaacgcca cctccagcgc
     1561 gattgtgaaa tatgtttccc agcgtgccgg gatcggcatc aacgccggac gtattcgtgc
     1621 gctgggcagc ccgattcgcg gtggcgaagc gttccatacc ggctgtatcc cgttctacaa
     1681 acatttccag acagcggtga aatcctgctc tcagggcggt gtgcgcggcg gtgcggcaac
     1741 gctgttctac ccgatgtggc atctggaagt ggaaagcctg ctggtgttga aaaacaaccg
     1801 tggggttgaa ggcaaccgcg tgcgtcatat ggactacggg gtacaaatca acaaactgat
     1861 gtatacccgc ctgctgaaag gtgaagatat caccctgttc agcccgtccg acgtaccggg
     1921 gctgtacgac gcgttcttcg ccgatcagga agagtttgaa cgcctgtata ccaaatatga
     1981 gaaagacgac agcatccgca agcagcgtgt gaaagccgtt gagctgttct cgctgatgat
     2041 gcaggaacgt gcgtctaccg gtcgtatcta tattcagaac gttgaccact gtaataccca
     2101 tagtccgttt gatccgacca tcgcgccagt tcgccagtct aacctgtgcc tagaaattgc
     2161 cctgccgacc aaaccgctga acgacgtcaa cgacgagaac ggtgaaatcg cgctgtgtac
     2221 gctgtctgct ttcaacctgg gcgcaattaa tagcctggat gaactggaag agctggcgat
     2281 tctggcggtt cgtgcacttg acgcgctact ggattatcag gattacccga tcccggccgc
     2341 caaacgtgga gcgatggggc gtcgtacgct gggtattggt gtgattaact tcgcttacta
     2401 tctggcgaag cacggtaaac gctactccga cggcagcgcc aacaacctga cgcataaaac
     2461 cttcgaagcg attcagtatt acctgctgaa agcgtctaat gagctggcta aagagcaagg
     2521 cgcgtgcccg tggtttaacg aaaccactta cgcgaaaggg atcctgccga tcgataccta
     2581 taagaaagat ctggatgcca tcgctaatga gccgctgcat tacgactggg aagcattgcg
     2641 tgaatcaatc aaaacgcacg gcctgcgtaa ctccacgctt tctgccctga tgccgtccga
     2701 gacctcttcg cagatctcta acgccactaa cggtattgaa ccgccgcgcg gttacgtcag
     2761 catcaaagcg tcgaaagacg gtattttgcg ccaggtggtg ccggactacg agcacctgca
     2821 cgacgcctat gagctgctgt gggaaatgcc gggtaacgat ggctacctgc aactggtagg
     2881 tatcatgcag aaatttatcg atcagtcgat ctctgctaac accaactacg atccgtcacg
     2941 tttcccgtca ggaaaagtgc cgatgcagca gttgctgaaa gacctgctca ccgcctacaa
     3001 attcggggtc aaaacgctgt attatcagaa cacccgtgac ggcgctgaag acgcgcagga
     3061 cgatctggta ccgtcaatcc aggacgatgg ctgcgaaagc ggcgcatgta agatctgata
     3121 ttgagatgcc ggatgcggcg taaacgcctt atccggccta cggctcggtt tgtaggcctg
     3181 ataagacgcg ccagcgtcgc atcaggctcc gggtgccgga tgcagcgtga acgccttatc
     3241 cggcctacgg ctcggatttg taggcctgat aagacgcgcc agcgtcgcat caggcacagg
     3301 atgcggcgta aaatgcctta tccggcatta aactcccaac aggacacact catggcatat
     3361 accacctttt cacagacgaa aaatgatcag ctcaaagaac cgatgttctt tggtcagccg
     3421 gtcaacgtgg ctcgctacga tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag
     3481 cagctctctt tcttctggcg tccggaagaa gttgacgtct cccgcgaccg tatagattac
     3541 caggcgctgc cggagcacga aaaacacatc tttatcagca acctgaaata tcagacgctg
     3601 ctggattcca ttcagggtcg tagcccgaac gtggcgctat tgccgcttat ttctattccg
     3661 gaactggaaa cctgggtcga aacctgggcg ttctcagaaa cgattcattc ccgttcctat
     3721 actcatatca ttcgtaatat cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc
     3781 aacgagcaga tccagaaacg tgcggaaggg atctccagct attacgatga actgatcgaa
     3841 atgaccagct actggcacct tctgggcgaa ggcacccaca ccgttaacgg taaaaccgta
     3901 accgttagcc tgcgcgagct gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg
     3961 ctggaagcga tccgcttcta cgtcagcttt gcttgttcct ttgcctttgc agaacgcgaa
     4021 ctgatggaag gtaacgccaa gattattcgc ctgattgccc gcgatgaagc cctgcacctg
     4081 accggtactc agcatatgct gaatctgctg cgcagcggcg cggatgatcc ggagatggcg
     4141 gaaattgccg aagaatgtaa gcaggagtgc tatgacctgt ttgtccaggc ggcacagcaa
     4201 gagaaagact gggcggatta tctgttccgt gacggttcga tgattggtct gaataaagat
     4261 attctctgcc agtacgttga atacatcacc aatatccgca tgcaggcagt cggtttggat
     4321 ctgccgttcc agacgcgctc caacccgatc ccgtggatca acacctggct ggtgtctgat
     4381 aacgtgcagg ttgctccgca ggaagtggaa gtcagttctt atctggtcgg gcagattgac
     4441 gcggaagtgg acaccgacga tttgagtaac ttccagctct gatggcccgc gttaccctgc
     4501 gcatcactgg cacacaactg ctgtgccagg atgaacaccc ttcccttctg gcggcgctgg
     4561 aatcccacaa tgtggcggtt gagtaccagt gccgcgaagg ttactgcggc tcctgtcgca
     4621 cccgactggt cgcaggtcag gtggactgga ttgccgaacc gttagccttt attcagccgg
     4681 gggaaatttt gccctgttgt tgccgggcaa aaggcgatat tgaaatcgag ttgtgatcgg
     4741 gtactagcgc cagatacgac gcttacgcgt attatctggc ctacaacgat tacatggcgt
     4801 agtaatacgc tttcacctgc tcccagtccg ctttagggat cggctccaga tatttttcca
     4861 gctggcggaa gtcatgatta atcgctttat cgcgacgcaa gcggcgacga cttttctcca
     4921 gatcaagaaa accagcttct gccttacctt ctgttttcac atagatatgg cgaacatagc
     4981 aacagccatg ctgacgatta acgctatgca ttttcttaaa cgccagtgcc actgctttca
     5041 acatggcttg ccgtacttcg tcagaataag gcgatactgc atgccgggca taccagtcag
     5101 caatgctgat gaaccccgcc atatcttcag tcaccagcaa cgcgcgccat tcaccctcaa
     5161 ttttcaccgc ttcaccaaaa acgatcttcg gcacgatgac accggcccgt tccagttctt
     5221 taattaccgc aacctcacgg acaatcgttg gtcggccgaa cggataacgt actgaatgaa
     5281 acagatgatg cgtcatgcgc tttacataca gctttttgcc gttgcgctcg acgcattgca
     5341 ccccgctcat accattacgg cgatagttag gctcttcaac ccagtcgccc tctgttgccc
     5401 accagtgatt aaattcgtcg tactttgctg aaactgccat accctatcgc ctgtcatttt
     5461 tattaacgac aatgactata ggtggttacc tgaggaaaat cttaatgaaa cgtgtcgtat
     5521 taatgaattt cttttgccgc tacaggtgtg acaccgcttt tgcccttcat ttgcagcgca
     5581 tagattccac cagccactac cactaatagt gcgcccagca gataaaatgt gaatttaatc
     5641 agtcggatac agcgatgaat tttattcgac agggattagg catcgcctta caaccagagt
     5701 taacgctgaa aagcattgca ggtgaattgt gttccgttcc tcacgaacca accttctatc
     5761 gacagatttc gttgctgact aaagaaaagc cggtagaagg cagtccactg tttttactac
     5821 aaatgtgcat ggaacaatta gtggcgattg gaaaaatttg atatgagaca ggatggcgca
     5881 tgaacgccat cctgcagtaa cattactctt tattaagaaa ttttactgcc ttatcaggga
     5941 aatcagtaaa cagcccattt acacccgctt tgttatacag agcatcatat aactgattca
     6001 catcaggagt gtattcaggc agtttatctg accgcacggt ataaggatgc actaccagtt
     6061 tattctgctg ggcatcttgc accatgccag tgagtttgat attacccggc tgcgatgtct
     6121 cctcaatcaa catatggtaa tccggaccaa taccatctgc atattccgcc acctgtttca
     6181 tggctcccgg cttaaacatc cagtcgtagt tgtaattaac ccagcttcca tccggctgtt
     6241 tctgctgcgt ttcattccag tcggtatagg caatcagctg taccagattg agctccatgc
     6301 ccattttggg ttccagctca ttcttaatac gcttcagctc atcagcatca aaacattgca
     6361 aataaacttt atcgtcttta ccggtgtaac catatttctt cagcacttcc agcgtttttg
     6421 ccgcaatatc cttcccttcc tgatgatgga accacggcgc tttgatttct ggatagatac
     6481 cgatattttt cccggtagag tgatttaacc cctgaacaaa ttcaatctct tcttcaaagg
     6541 tgtgcacccg gaagtcggac ttacccattg ggaaacgccc cggataagtc tgtacttttt
     6601 taccgttttc aatatcgaaa ccttcggtaa atttcagcga cttaatttca tccagcgtga
     6661 aatctatcgc gtagtaacga ccgtctttgc gcgcccgatc cgggaaacga tcggcaacat
     6721 cagtaacacg atcgagataa tggtcatgca gaacaaccag atggtcgtct ttggtcatca
     6781 ccaaatcctg ttccagataa tccgctccct gcgcatacgc catcgctttt gctggcagcg
     6841 tatgctccgg caaatatcca ctggcaccgc gatgggcgat gactattttt tcgttgctgt
     6901 ccgccgccat tgcactgctt cccatgacta tagtgctcat catgatcgcc atgctaaggt
     6961 ttttcagctt caatttcatg ccattagcct ccgttgcgtt tttgcagtaa ttgttcatgg
     7021 cgacgttttt cgccaatcat cacaacaatc aacaagataa ccgccagaat gctgccgcca
     7081 atcattacca taaagccgcc atcccagccg aagaagtcca cggtgtagcc aacaatcgcg
     7141 ctcgccgcca ccgaaccgcc caggtaacca aacagcccgg taaagcccgc tgccgtacct
     7201 gccgcttttt tcggtgccag ttccagcgca tgcagaccga tcagcatcac aggaccgtag
     7261 atcaggaagc cgataacaat catacaaatc atatcgacgg ttgggttacc tgccgggttc
     7321 atccagtaaa cgatagtcgc gatggtcacc agtgtcataa agaagacgcc ggttgcccca
     7381 cggttaccac ggaagacttt gtccgacatc cagccgcaca gcagggtgcc cggaataccc
     7441 gcatactcat agaagaagta ggcccaggag gatttatcca gcgcgaaatg cttaacctct
     7501 ttcagataag tcggtgacca gtcgaggatg ccgtaacgca gcagataaac gaacacgttg
     7561 gcgatggcga tgtaccacag cagtttgttc ggcagcacgt actgcatgaa gatttgcttc
     7621 gcggtcagct cctgttccgc tttttcgtta tagtcgtccg gataatcatt tttgtactct
     7681 tcgatcggcg gcaagccaca ggattgcggg gtatcgcgca tcatcgcaaa ggcgaataat
     7741 gccaccagaa tggcgcagaa agcaggcata tagagcgccg catgccagtc attgaaccag
     7801 gccatcccca gcaggaacag cagcggcgga ataccaccac cgacgttgtg cgcacagttc
     7861 cacactgaca caatgccgcc acgttctttc tgcgaccacc agtgcaccat agtacgacca
     7921 cacggcggcc accccatccc ctggaaccaa ccgcagagga acaacagtac aaacatcacc
     7981 gcaatgctcg acgtcgccca tggcacaaag cccataaaca acatcactgc cgccgccaga
     8041 atcaaacctg cgggcaggaa aacgcgcgga ttcgagcgat ccgataccga acccatgatg
     8101 aatttcgaaa atccataagc aatcgagatc cccgaaaggg caaaacctaa atcaccgcgt
     8161 gagaatccct gctcaaccag ataaggcata gcaagcgcaa agttcttacg aaccaaatag
     8221 taagccgcat agccaaagaa tatccccagg aaaatttgcc agcgcaatcg acgataagtc
     8281 ggatcgatct ccgcggcagg taagcgcgct ttgtgtggcg ctggtttaaa aatactcaac
     8341 attgatagcc tccgtggccc gtggtcttat ttatgattaa cagcctgatt cagtgagaga
     8401 acctgccgtt tcttgagttg ccgcgatgtt aagaaaacat tcataaatta aatgtgaatt
     8461 gccgcacaca ttattaaata agatttacaa aatgttcaaa atgacgcatg aaatcacgtt
     8521 ttactttcga atcatgagcg aatatgcgcg aaatcaaaca attcatgttt ttactatggc
     8581 taaatggtaa aaaacgaact tcagagggat aacaatgaaa actcgcgact cgcaatcaag
     8641 tgacgtgatt atcattggcg gcggcgcaac gggagccggg attgcccgcg actgtgccct
     8701 gcgcgggctg cgcgtgattt tggttgagcg ccacgacatc gcaaccggtg ccaccgggcg
     8761 taaccacggc ctgctgcaca gcggtgcgcg ctatgcagta accgatgcgg aatcggcccg
     8821 cgaatgcatt agtgaaaacc agatcctgaa acgcattgca cgtcactgcg ttgaaccaac
     8881 caacggcctg tttatcaccc tgccggaaga tgacctctca ttccaggcca cttttattcg
     8941 cgcctgcgaa gaagcaggga tcagcgcaga agctatagac ccgcagcaag cgcgcattat
     9001 cgaacctgcc gttaacccgg cactgattgg cgcggtgaaa gttccggatg gcaccgttga
     9061 tccatttcgt ctgaccgcag caaacatgct ggatgccaaa gaacacggtg ccgttatcct
     9121 taccgctcat gaagtcacgg ggctgattcg tgaaggcgcg acggtgtgcg gtgttcgtgt
     9181 acgtaaccat ctcaccggcg aaactcaggc ccttcatgca cctgtcgtgg ttaatgccgc
     9241 tgggatctgg gggcaacaca ttgccgaata tgccgatctg cgcattcgca tgttcccggc
     9301 gaaaggatcg ctgctgatca tggatcaccg cattaaccag catgtgatca accgctgccg
     9361 taaaccttcc gacgccgata ttctggtgcc tggcgatacc atttcgctga ttggtaccac
     9421 ctctttacgt attgattaca acgagattga cgataatcga gtgacggcag aagaggttga
     9481 tattctgctg cgtgaagggg aaaaactggc ccccgtgatg gcgaaaacgc gcattttgcg
     9541 ggcctattct ggcgtgcgcc cgctggttgc cagcgatgac gacccgagcg gacgtaacgt
     9601 cagccgtggc atcgtgctgc tcgaccatgc tgaacgcgat ggtctggacg gatttatcac
     9661 catcaccggt ggcaaactga tgacctatcg gctgatggct gaatgggcta ccgacgcggt
     9721 atgccgcaaa ctgggcaaca cgcgcccctg tacgactgcc gatctggcac tgcctggttc
     9781 acaagaaccc gctgaagtta ccttgcgtaa agtcatctcc ctgcctgccc cgctgcgcgg
     9841 ttctgcggtt tatcgtcatg gcgatcgcac gcctgcctgg ctgagcgaag gccgtctaca
     9901 ccgtagcctg gtatgtgagt gcgaagcggt aactgcgggt gaagtgcagt acgcggtaga
     9961 aaatttaaac gttaatagcc tgctggattt acgccgtcgt acccgtgtgg ggatgggcac
    10021 ctgccagggc gaactctgcg cctgccgcgc tgccggactg ctgcaacggt ttaacgtcac
    10081 gacgtccgcg caatctatcg agcaactttc caccttcctt aacgaacgct ggaaaggcgt
    10141 gcaacccatc gcctggggag acgcactgcg cgaaagcgaa tttacccgct gggtttatca
    10201 gggactgtgt ggtctggaga aggagcagaa agatgcgctt tgatactgtc attatgggcg
    10261 gcggcctcgc cggattactc tgtggcctgc aactgcaaaa acacggcctg cgctgtgcca
    10321 ttgtcacgcg tggtcaaagc gcgttgcatt tctcatccgg ttcgctggat ttgctgagcc
    10381 atctgccaga tggtcaaccc gtggcagaca ttcacagtgg actggaatct ttgcgtcagc
    10441 aggcacctgc acatccttac tctcttctcg ggccacaacg cgtgctcgat ctcgcttgcc
    10501 aggcgcaggc attaatcgct gaaagcggtg cgcaattgca gggcagcgta gaactggctc
    10561 accagcggat tacgccgctc ggcactttgc gtgcaacctg gttaagttcg ccagaagttc
    10621 ccgtctggcc gctacccgcg aagaagatat gtgtagtggg gataagcggg ctgatggatt
    10681 tccaggcgca tcttgcggcg gcttcgttgc gcgaactgga tctttcggtt gaaaccgcag
    10741 aaatagagct gccggaactg gatgtcctgc gcaataacgc caccgaattt cgcgcggtga
    10801 atatcgcccg tttccttgat aatgaagaaa actggccgct gttacttgat gcgcttattc
    10861 ctgtcgccaa tacctgcgaa atgatcctga tgcccgcctg cttcggcctg gccgatgaca
    10921 aactgtggcg ttggttgaat gaaaaactac cttgttcact gatgcttttg ccaacgctgc
    10981 cgccttccgt gctgggcatt cgtctgcaaa accagttaca gcgccagttt gtgcgccaag
    11041 gtggagtgtg gatgccgggc gatgaagtga aaaaagtgac ctgtaaaaat ggcgtagtga
    11101 atgaaatctg gacccgcaat cacgccgata ttccgctacg tccacgtttc gcggttctcg
    11161 ccagcggcag tttctttagt ggcggactag tagcggaacg taacggcatt cgcgagccga
    11221 ttctcggcct tgatgtgcta caaaccgcca cgcggggtga atggtataag ggcgattttt
    11281 ttgcgccgca accgtggcag cagttcggtg taaccactga tgagacgcta cgcccgtcgc
    11341 aggcagggca aaccattgag aacctgtttg ccatcggttc ggtgctgggc ggatttgatc
    11401 ccatcgccca gggatgcggc ggcggtgttt gtgccgtcag tgctttacat gccgctcaac
    11461 agattgccca acgcgcagga ggccaacaat gaacgacacc agcttcgaaa actgcattaa
    11521 gtgcaccgtc tgcaccaccg cctgcccggt gagccgggtg aatcccggtt atccagggcc
    11581 aaaacaagcc gggccggatg gcgagcgtct gcgtttgaaa gatggcgcac tgtatgacga
    11641 ggcgctgaaa tattgcatca actgcaaacg ttgtgaagtc gcctgcccgt ccgatgtgaa
    11701 gattggcgat attatccagc gcgcgcgggc gaaatatgac accacgcgcc cgtcgctgcg
    11761 taattttgtg ttgagtcata ccgacctgat gggtagcgtt tccacgccgt tcgcaccaat
    11821 cgtcaacacc gctacctcgc tgaaaccggt gcggcagctg cttgatgcgg cgttaaaaat
    11881 cgatcatcgt cgcacgctac cgaaatactc cttcggcacg ttccgtcgct ggtatcgcag
    11941 catagcggct cagcaagcac aatataaaga ccaggtcgct ttctttcacg gctgcttcgt
    12001 taactacaac catccgcagt taggtaaaga tttaattaaa gtgctcaacg caatgggtac
    12061 cggtgtacaa ctgctcagca aagaaaaatg ctgcggcgta ccgctaatcg ccaacggctt
    12121 taccgataaa gcacgcaaac aggcaattac gaatgtagag tcgatccgcg aagctgtggg
    12181 agtaaaaggc attccggtga ttgccacctc ctcaacctgt acatttgccc tgcgcgacga
    12241 atacccggaa gtgctgaatg tcgacaacaa aggcttgcgc gatcatatcg aactggcaac
    12301 ccgctggctg tggcgcaagc tggacgaagg caaaacgtta ccgctgaaac cgctgccgct
    12361 gaaagtggtt tatcacactc cgtgccatat ggaaaaaatg ggctggacgc tctacaccct
    12421 ggagctgttg cgtaacatcc cggggcttga gttaacggtg ctggattccc agtgctgcgg
    12481 tattgcgggt acttacggtt tcaaaaaaga gaactacccc acctcacaag ccatcggcgc
    12541 accactgttc cgccagatag aagaaagcgg cgcagatctg gtggtcaccg actgcgaaac
    12601 ctgtaaatgg cagattgaga tgtccacaag tcttcgctgc gaacatccga ttacgctact
    12661 ggcccaggcg ctggcttaaa ctcccttctg atgcccggta agcatgtggt taccgggcat
    12721 ttttgcgtac acgattccgt gcccaatgta tgcgttgcaa cgcagtgaaa attcctctga
    12781 aaacgtctcg caaaggctga aactggcaga tgtcaaaggc ctgggataac cgtaatgtcg
    12841 cgtcatcata aatatcaggt gacggacaac catgaccgaa tcaacaacct cctccccgca
    12901 tgatgcggta tttaaaacct ttatattcac acccgaaacc gcacgggatt ttctcgaaat
    12961 acatttacca gaaccactgc gcaagctttg caacctgcaa accttacgcc tggaacccac
    13021 tagttttatt gaaaaaagtt tacgcgctta ctactcggat gttttgtggt ccgtggaaac
    13081 cagcgacggt gacggctata tctactgcgt gattgaacat caaagctctg cagaaaagaa
    13141 tatggctttt cggccaatgc gctatgccac tgccgccatg cagagtcacc tagataaagg
    13201 ctatgacaga gttccgctgg tggtgccatt gctgttttat catggcgaaa catcgcccta
    13261 cccgtactca cttaactggc tggatgagtt tgacgatccg caacttgccc ggcagttgta
    13321 caccgaagct tttccgttgg tggatatcac catcgtacct gacgatgaga tcatgcaaca
    13381 tcggcgtata gctctgctgg aactgattca aaagcatatt cgcgaccacg atttaatcgg
    13441 catggtcgac aggatcacca cgcttttggt tagaggcttc actaatgaca gccagctaca
    13501 aacactgttt aattatctgc tgcaatgcgg cgatacctcc cgtttcaccc gttttattca
    13561 ggagattgcc gaacgttcac cactacaaaa ggagagatta atgactattg ctgaacggct
    13621 acggcaggaa ggacatcaaa ttggctggca agaaggtaaa ttagtaggtt tgcagcaagg
    13681 taaattagaa ggtttgcagg aaggtatgca tgaacaagcc attaaaattg ctttgcgcat
    13741 gctggaacag ggcattgatc gtgaccaagt gctcgcggcc acccagctaa gcgaagccga
    13801 tctggcagcg aataaccact aattaacaca ggcccacagc cgatccccat gggcctttga
    13861 tatcaataac taccttttat gcgtggccca tttttgcctg atttaaacat cgccagtcgt
    13921 tgatccaggg catcgctgta gagcatcgtg tcaacgccaa cagcgacaaa gttcgctccc
    13981 cacgccaggc attgctgcgc catatcagga gccacagcca gaaaaccagc cgctttaccc
    14041 gcagcacgga tccgccgaat gctggtttca ataattcgct gcacttccgg gtgcccggcg
    14101 ttatccgggt agcccaacga cgcagaaaga tccgctggtc caataaacac gccatcaatc
    14161 ccttcgacgt cgaggatctc gtccaggtta tccagtgccg ttttactttc cacctgcact
    14221 aacagacaaa gcgaatcgtt gacttgcgcc atgtaattct caatgcgtcc ccagcgcgca
    14281 gcccgtgcca cactggcccc aacaccacgc tcaccgtagg gagggttgga caaattttgc
    14341 cggaggggga atgtgccggt gcagtaaacc ggcacagagt ggcgaaatca gacggataaa
    14401 gtcatggact caacaacctc aatccagcca tgctcactag cgatgtcctg gccgtttaac
    14461 caacggcgta gcatattcag tgccatcatt gcgcacactt cctgacggat agccaggctg
    14521 tagcgtgtag tgctgaaacg cacacgcaga gcgaaagtgc cgtctggcgt ggctagcgca
    14581 aagttgagat gctcgttctc gaaacccgaa acagccagtg ccagcccggc aaaatggttg
    14641 gcccgccgtt ctgtaatcca gtgcgcagtt tgcgccaggg tttcttcctg tgaaggaatc
    14701 acttcacacg ccagcaatgg agcacctgcg cgagaaagtt gcaaagccaa taaaccaccg
    14761 gtgaattgct cgctcaacgt caggctgaac tggcggtttt gcaattcgcg actgatctgc
    14821 gcgggcagtc cttcggtgcc ttcgaaaatc acgctctgtc cggcaacacg tttaacgtcc
    14881 agccacagtt tttccatcgc ctgttgctcg cttgccggtc cggtgagttt cagttcgatg
    14941 atcggcattg aggagcgata gcccattgtt acgcccggcg gcagttgtag agtgtccagg
    15001 ctttgtgcca gatcgctttc cgaacgacca aaggtagtta aacgcagaca aaccggcggc
    15061 tgtggtaaag aaaaacgctc gcgcaggcgc ggcaggattt cgtgctcgac catcacctta
    15121 aattctgacg gtacgccggg agtaaagaac atcaggcaac gattaagctg cacggcaaaa
    15181 ccacaggcgg tgcctaccgg gttattgata aattcagcac tggcaggcag ctccgcttgt
    15241 ttacggttgc tcggtgccat tactcgtcca cgttcgtgga aatagcgttc catctctttg
    15301 agccaggctt catgcagcac caggccttca ccttttgctg tcgcagcggc gagtgcgctt
    15361 aaatcatcgc tggtcggtcc cagcccgccg ttaacgatca gcacatcggc gtgctgacta
    15421 cgttcgcgaa gaatggtgac taagtcatca aggttatccc ccaccgtatt gcggcgagat
    15481 aatggcagcc cctgatgaaa gaaaaaatcg gccagccagg cagcgttagt gtcaacgatt
    15541 tgcccgtata acacttcatc cccggtggat agcatttcca cttttaacat ggttatctcc
    15601 tgcttttgtt atcgaatcac tataacgcaa agggagataa ggcgtagtaa gaaactgaat
    15661 gccgcgccgg aagggatcag aaactggcgt taaccccaac atacaggccg tcagccacag
    15721 cgttgtcgcg gttaccgtct ttacccgaca gattcaggta gcgataaccc gcctcaatac
    15781 tgactggacg cataatggtg taacgcgcgc cagcattcgc ttcctcataa ctttgaatac
    15841 cgctggagag cgaatccgga gagtagtaat actcgccaaa caaacggaag ctgttgccaa
    15901 ttttccactg caaaccacct cctaccgctg cggcataacc ttcatcgccg taattcgggt
    15961 tggtgtacac gccttttccg ccaacggtcg ccattaacgg cccgagaggc agattcaacc
    16021 ccagccccac gcccgcgacg tcaccgtcgt cgtcgttatg tgtccagtta ccgcttaaag
    16081 ccaggcccgt cgattcagtc ccaaaaccga caccaatatt ggtgtattct ttacccgcct
    16141 ggccgctgat gctcattgca ttgaccgatg cagaaaccaa cagcattccg gccagacctg
    16201 ctagcgcaat ttttttcatt atcccctttc ccacaagcaa ttaatcaaaa ttcccaaagc
    16261 tgcgggattg tactgcctgg cacagaggat tcaatgagcg aaaaaaggga tctagtcact
    16321 tattgtaact aaatacttct taaataatta aacgccgaaa aaataaacac actttaccga
    16381 tacacacaaa aataataata agtgcagtta tcattgcaaa agagattact gtcactttca
    16441 catatttctg agttaccgta ttctcatggt ttctttttat ttataaggaa tacccctgtg
    16501 cgacaacgga ctattgtatg ccctttgatt caaaatgatg gtgcttattt gctgtgcaaa
    16561 atggccgacg atcgcggcgt tttccccggt caatgggcgc tttcgggtgg cggcgtggag
    16621 tctggcgaac gaattgaaga ggcactacgc cgcgaaattc gcgaagaact gggagaacag
    16681 ctgcttttga cagaaatcac gccgtggacc ttcagcgatg atattcgcac caagacatat
    16741 gcagatggtc gcaaggaaga gatttatatg atttacctga tttttgactg cgtttctgcc
    16801 aaccgagaag tgaaaataaa cgaagagttt caggactacg cgtgggtaaa acctgaagat
    16861 ctggtgcatt atgatttaaa tgttgccacc cgaaaaacgt tacgtttgaa aggtcttctt
    16921 taacaatgat gacagccatc actggctgtc agattgaaaa tttagtggtt aacgaattcc
    16981 ccatccagat aaactttgcc tttttccaca tgcatgacta aaccatccag ataatcaggt
    17041 ttaaatatcg catcacgctt atttttagca atatatgtca ggcaatgatt atgggtgaaa
    17101 ataacgatat ttttatcagg cgctttgttt tgtaagtcct taattgcact ataaatctca
    17161 ttaccgcact gaagaagtcg tttatctacc gtcagctttt tacccgcgga aaaccaggtc
    17221 gccgactgaa tggtccggac ggtattactg gaataaagat cgaaatcaga gatgtcagcg
    17281 ctaaaagcgt tgcccagttc acgggcatcc tgggtacctt taaccgtaat acctgtttta
    17341 tctgacaagc attgattggt tgaacgatcg caacgttcag catggcgaaa caaaacgaca
    17401 accgggtgct gctgtgccag tctggccaat gttttattgt cgatacgtgg caaaccattg
    17461 ctactccagg cggcatgagt acccagtccg gcaattgcag cgagcgccag taaaatgatg
    17521 aaatattttt ttgacttcaa tgatgagcgg caaaaagcta acacgtaaac tccacctata
    17581 gacaagcgca accagacaat taccgtgaaa ttgagcgaca tttctggcga taattagcag
    17641 ttggtgtaat attaaaaatc ctacgatgtc ggcaaaatgc ctcaaatttt tgccaaatgc
    17701 aaagcctaaa taagaaaaaa tataaaaatt tcaatattta cgtctaatat tagtttctta
    17761 aggttaagtt aatattctat ccttaaaatt tcgctccaaa tggcaaaata tacacaacac
    17821 tctttatagc aaatataagt ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa
    17881 tttttgcctt tttcgcgacc agcaatgggc gtggaggaac tcgctgcagt taaagaggtt
    17941 ctcgaatccg gttggatcac aaccggtccg aagaatcagg cgcttgagca agccttttgc
    18001 cagttgacgg gaaatcagca tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc
    18061 acgctaatgg cgttggaaat tggcaagggc gatgaagtga ttacgccttc cctgacctgg
    18121 gtttcaaccc tcaatatgat ttccttgctg ggtgcaacgc cggtaatggt ggatgtcgac
    18181 cgcgatacgc tgatggtcac gcctgaagcg atcgaatcag ccattacgcc acgcactaaa
    18241 gccatcattc cggtgcatta tgccggtgcg ccagcagata ttgacgccat tcgcgccatt
    18301 ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc atgccgtcgg tacgtattac
    18361 aaagggcgac atattggcgc aaaaggtacc gctatttttt catttcatgc cattaaaaat
    18421 attacctgtg ctgaaggtgg cctgattgta actgataatg aaaaccttgc ccgccagcta
    18481 cggatgctga aatttcacgg tctgggtgtc gatgcctatg acagacaaac ctggggccgt
    18541 gcaccgcagg ctgaagtctt aacaccgggc tataagtaca atctgaccga tattaacgcc
    18601 gcgattgccc tgacacagtt agtcaaatta gagcacctca acacccgtcg gcgcgaaatt
    18661 gcccagcaat atcagcaagc actggcagct ctcccctttc agccattaag ccttcccgcc
    18721 tggccgcacg ttcacgcctg gcatctgttt attattcgtg tcgatgaaca acgttgtggt
    18781 atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag gcattggtac cgggttacat
    18841 ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt ttcccacgct gtcgttaccg
    18901 aataccgaat ggaatagcga acgcatctgt tcgttgccgc tgttcccgga tatgactacc
    18961 gccgatgccg accgcgtcat cacagccctt cagcaactcg caggacaata agccatgttt
    19021 gaaatccacc ctgttaagaa agtctcggtg gttattcccg tttataacga gcaggaaagc
    19081 ttaccggaat taatcaggcg caccaccaca gcctgtgaat cgttggggaa agagtatgag
    19141 atcctgctga ttgatgacgg cagtagcgat aattccgcgc atatgctggt cgaagcctca
    19201 caagcggaga acagccatat tgtgtctatt ttgattaacc gcaattacgg gcaacattca
    19261 gcgattatgg cgggattcag tcacgttact ggcgacttaa ttattaccct tgatgccgat
    19321 ctccagaatc cgccagaaga aatcccccgc ctggtggcaa aagccgatga aggttacgac
    19381 gtggtaggga ctgtacgcca gaaccgccag gacagctggt ttcgtaaaac agcttcgaag
    19441 atgattaacc ggcttattca gcgcaccact ggcaaagcga tgggtgacta cggttgtatg
    19501 ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc actgccatga acgcagcacc
    19561 tttatcccga ttctggcgaa tatcttcgcc cgccgtgcaa ttgaaattcc agtacatcat
    19621 gccgagcgtg agtttggtga atccaaatac agttttatgc gcctgattaa tttgatgtac
    19681 gacctggtga cctgccttac cacaacgccg ctacgtatgc tgagtctgct cggcagcatt
    19741 attgcgattg gaggttttag cattgcggtg ctgctggtga ttttacgcct gaccttcgga
    19801 ccacaatggg cggcagaagg cgtctttatg ctatttgccg tgctgtttac ttttattggc
    19861 gctcagttta tcggcatggg attactcggt gaatatatcg gcaggatcta caccgatgtc
    19921 cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa
    19981 taatgaaaac cgtcgttttt gcctaccacg atatgggatg cctcggtatt gaagctctgc
    20041 tggctgccgg ttacgaaatt agcgccattt ttacccatac cgataatccc ggtgaaaaag
    20101 ccttttatgg ttcggtggct catctggcgg cggaaagaga cattccggtt tatgcgccgg
    20161 ataacgttaa tcatccgctg tgggtggaac gcattgccca actgtcacca gaggtgattt
    20221 tctcttttta ttatcgccat cttatttgcg acgagatttt tcagctcgct cccgcaggtg
    20281 catttaatct gcacggttcg ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg
    20341 tgctggtgaa cggtgaaacg gaaacgggcg ttacattgca ccgaatggta aacgtgccga
    20401 tgctggggcc attgtggcgc aactgcgcgt tgccattgcg ccagacgata tcgctattac
    20461 gctgcatcat aaattgtgcc atgccgcgcg ccagctactg gaacagacat tacccgccat
    20521 taaacacggt aatattctgg aaatcgccca gcgcgaaaac gaagccacct gttttggtcg
    20581 cagaacgccg gatgacagtt tccttgaatg gcataaaccg gcatccgtac tgcacaacat
    20641 ggtacgtgcc gttgccgatc cgtggccggg tgccttcagc tatgttggca atcagaaatt
    20701 caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat
    20761 ttctgttgcg ccactgctga ttgcctgtgg cgatggcgcg ctggaaatcg tcaccggaca
    20821 ggcgggcgac ggcattacta tgcagggctc gcaattagcg cagatgctgg gcctggtgca
    20881 aggttcacgc ttgaatagcc agcctgcctg caccgcccga cgccgtaccc gggtactcat
    20941 cctcggggtg aatggcttta ttggcaacca tctgacagaa cgcctgctgc gcgaagatca
    21001 ttatgaagtt tacggtctgg atattggcag cgatgcgata agccgttttc tgaatcatcc
    21061 acattttcac tttgtcgaag gcgatatcag tattcattcc gaatggattg agtatcacgt
    21121 caaaaaatgt gatgtcgtct tgccgctggt ggcgatagcc acgccgattg aatatacccg
    21181 caacccgctg cgcgtatttg aactcgattt tgaagagaat ctgcgcatta tccgctactg
    21241 cgttaagtac cgtaagcgaa tcatcttccc gtcaacttca gaagtttatg ggatgtgtag
    21301 cgataaatac ttcgatgagg accattctaa tttaatcgtc ggcccggtga ataaaccacg
    21361 ctggatttat tcggtgtcaa aacaattact tgatcgggtg atctgggcct atggcgaaaa
    21421 agagggttta cagttcaccc tcttcctccc gtttaactgg atgggaccac gactggataa
    21481 ccttaatgca gcgcgaattg gcagctcccg cgctattacg caactcattc tcaatctggt
    21541 agaaggttca ccaattaagc taattgatgg cggaaaacaa aaacgctgct ttactgatat
    21601 ccgggacggt atcgaggcgt tgtaccacat tatcgaaaat gcgggaaatc gctgcgacgg
    21661 cgaaattatc aacattggca atcctgagaa cgaagcgagc attgaggaac tgggcgagat
    21721 gctgctggcg agcttcgaaa aacatccgct gcgccatcat ttcccaccgt ttgcgggctt
    21781 tcgcgttgtc gaaagtagct gctactacgg caaaggatat caggacgtag agcatcgtaa
    21841 accgagcatc cgcaatgccc accgctgcct ggactgggag ccgaaaattg atatgcagga
    21901 aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg ataaaccatc
    21961 atgaccaaag taggcttacg cattgatgtc gatgcctttc gtggcacccg tgaaggcgtg
    22021 ccgcgtctgc tggaaatctt gagtaagcat aatattcagg ccagcatttt tttcagcgtc
    22081 ggcccggaca atatgggccg ccatctctgg cgactggtga agccacagtt tttgtggaag
    22141 atgctgcgct caaacgcggc atcgctttat ggctgggata ttttactggc aggtacggcc
    22201 tggccaggta aagagattgg tcatgccaat gccgatatca ttcgtgaagc ggctaaacat
    22261 cacgaagtcg gtctgcacgc ctgggatcac catgcctggc aagcccgtag cggtaactgg
    22321 gatcggcaaa caatgatcga cgatattgca cgcggtcttc gcactctgga agagattatc
    22381 ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg ccgaccagaa ggtgatcgaa
    22441 gcaaaagaag cgttccattt gcgctacaac agcgattgtc gtggggccat accgttccgt
    22501 ccactgcttg aatcaggaaa ccctggcact gcgcaaattc cggtgacctt acccacctgg
    22561 gatgaagtga ttggtcggga tgtgaaagca gaagatttta acggttggtt actcaaccgc
    22621 atcctgcgag ataaaggcac gccggtttat accattcatg cagaagttga aggctgcgct
    22681 tatcagcata attttgtgga tctcctcaaa cgcgcagctc aggaaggcgt gacattttgc
    22741 cctttaagcg aactgttatc agagacgttg ccgctcggac aagttgttcg cggaaatatt
    22801 gccggacgtg aaggctggct gggttgccaa caaattgcgg gtagtcgctg atgaaatcgg
    22861 tacgttacct tatcggcatc ttcgcgttta ttgcctgcta ttacctgtta ccgatcagca
    22921 cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga aatcagtcga gaaatgctgg
    22981 catccggcga ctggattgtg ccccatctgt tagggctacg ttatttcgaa aaacccattg
    23041 ccggatactg gattaacagc attgggcaat ggctatttgg cgcgaataac tttggtgtgc
    23101 gggcaggcgt tatctttgcg accctgttaa ctgccgcgct ggtgacctgg tttactctgc
    23161 gcttatggcg cgataaacgt ctggctctac tcgccacagt aatttatctc tcattgttta
    23221 ttgtctatgc catcggcact tatgccgtgc tcgatccgtt tattgcattc tggctggtgg
    23281 cgggaatgtg cagcttctgg ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag
    23341 gatttttact gctgggaatc acctgcggca tgggggtgat gaccaaaggt tttctcgccc
    23401 ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac gcaaaaacgc tggaaagatc
    23461 tctttattta cggctggctg gcggttatca gttgcgtact gacggttctc ccctggggac
    23521 tggcgatagc gcagcgggag cctgacttct ggcactattt tttctgggtt gagcatattc
    23581 aacgctttgc actagatgat gcccaacata gagctccgtt ctggtactac gtgccggtca
    23641 tcattgccgg tagcctgccg tggctgggat tactccccgg tgcactgtac acaggctgga
    23701 aaaaccgcaa gcattccgca accgtctatt tgttgagctg gacgataatg ccgctgctgt
    23761 ttttctccgt cgctaaaggc aaattgccca cctatattct ttcttgcttt gcacctctgg
    23821 caatgctgtt ggcgcattac gcgttgctgg cagcaaaaaa taatcctctg gcgctgcgga
    23881 ttaatggctg gattaacatc gcttttggcg tcactggcat tattgccaca tttgtgatct
    23941 ccccctgggg accaatgaac acgccggtgt ggcaaacctt cgagagctat aaagtctttt
    24001 gtgcctggtc gattttttcg ctatgggcat ttttcggctg gtacacctta acaaacgtcg
    24061 aaaagacctg gtctttcgcc gcgctttgcc cgctggggct ggcgttgctg gtaggatttt
    24121 caattcctga cagagtcatg gaaggaaaac atccgcaatt ttttgtcgag atgacacaag
    24181 aatcacttca gccaagccgc tatattctta ctgatagcgt cggtgttgcc gcaggtctgg
    24241 catggagcct gcaacgcgat gacatcatca tgtatcgcca gacaggtgag ttgaaatacg
    24301 gccttaatta tccggatgcg aaagggagat ttgtcagcgg tgatgagttc gcaaactggc
    24361 ttaatcaaca tcgtcaggag gggattatta cactcgtgct ttcggttgac cgcgatgaag
    24421 atatcaacag tctcgccatt ccgcccgcag atgccatcgt tcgtcaggag cgtctggtgc
    24481 tgattcagta tcgtcccaaa tgatctggct aacattagtc tttgccagct tgcttagcgt
    24541 tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg gcgataaaca aacggcgcaa
    24601 acatatcgtg ctgtggctgg gactggcgct ggcttgtctt ggtcttgcca tgatgctctg
    24661 gctgctggtc ttgcagaacg taccggtagg cattgcctac ccgatgttaa gtctgaattt
    24721 tgtctgggtg acgctggctg cagtaaaact gtggcacgaa ccggtatcgc cgcgtcactg
    24781 gtgtggggtg gcgttcatta ttggtggcat tgtgatcctc gggagtacgg tgtaatgtgc
    24841 ctgatatggg gattattcag cgtcattatt gcctcagttg cgcaattaag cctgggtttt
    24901 gcggcaagtc atctgccgcc gatgacgcac ttatgggatt ttatcgcggc tctgttagct
    24961 tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct ttccgttttt
    25021 tgttggtata aaacgctaca taaacttgcg ctaagcaaag cctatgcctt gctcagtatg
    25081 agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg gactttttcg
    25141 ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat tttcctgccc
    25201 acgacaaaac aacgttactg agttttccct gccgctgtac actgccgttc ccactcatcg
    25261 gggctataac tgcttgccga gaggactttc accgtatgca gcttttcacg attaatacaa
    25321 taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa gtcactcttc
    25381 acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataagtac atgtctgtta
    25441 tcttgtttat tgcagcacga ttttttgtgc cagccattcc attgcattat cctgtttgct
    25501 aagagttttc acatcaattt acagattaac agataaactc tttatcaacg gcaatggaat
    25561 ggcagaaatg agaagtgaaa cggagggtta attattgctg acgttgcacc cactctttta
    25621 gcgcctgacg tgaaatttta ataccgccgt ttttcagctc cggcggtaaa gtcagccagc
    25681 gcaccggttg ttgaaaacga gccagcttat ctttcaccca ttcgccaaga tcaacggttt
    25741 gctggtcata ctccaccacc gctaccggcc gatgaccaaa ctccttgtcg gcaacggaaa
    25801 cgataaacac ctgcaaaacc gcaggatgtg cagcaataac acgctcgact tcttccggct
    25861 gaataccctc accgccactg aagaacagat tgtctaaacg tccgacaatg gtcagcttgc
    25921 cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa
    25981 ccagttgccc gttacgccag taaccttctg ccatactggc agcccgcagc cacacttcat
    26041 tattaacgat tttcacttcc cgacccggca gcggcaaacc aacgtctgcc aggccgtcgg
    26101 cttctttcgc acataccgtg gaggcaaact cggtcagacc atagccgcaa aagcaacgaa
    26161 tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg ccaagtaaca
    26221 ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt gttggcacca
    26281 gtgaagcgtg agtacagcct gccagcattt gctccaatgg ctgtttatca cgcaccgtca
    26341 tccgcgcacc agcgtataac cagcgccaca taattccctg accggagacg tgaaacagcg
    26401 gtaaagagag caaccagtca tcgtgatcgc caaacggaat cagcgataac acaccttgcg
    26461 cactggcaag atgggcctga taagtatgga cagcggcttt cggcaaaccg gtagaacccg
    26521 aggtcaacgt cattgagcac agacgcgtcg gctgccacgc agcggcatgt acgccttcaa
    26581 ccagctgaat gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg
    26641 caaattgcag cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc agctgagggt
    26701 tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg
    26761 gcgtattcca cgcccgcaac atcacgccgc tgccctcaac caccccctgc gccgcaaagc
    26821 ccgtagctaa ctcatcgacg cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa
    26881 gacgtaaggc gatggcttct ccccgcactt gccgccagtg acgccacggc cagtcagaga
    26941 agatcataac aactgctcct gtacatcagc gtccactaac ggcagtgggc tccccggcca
    27001 gcgacgaacc tgctgcgcct gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg
    27061 cgttaaccag gcagcaatcc gcgccagttg cgttagacct aagctcgact caatggaaga
    27121 actgatcacc gccgtcagcc ccagcgcatg cgccgcctgt acctgctcgc gtactttttc
    27181 cagactgccc gtgagcgtgg gtttgataac taccgcgcgc acgccctctt cagccacaaa
    27241 ggcaaaatcc ggctcgcgca ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc
    27301 aaacgctcgc gaatcatcgc gggttttgca cggctcttcg agaaacgcga tgcggtggcg
    27361 ataatccggg ttaacatatt tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt
    27421 tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc aacagattca ccaccatgcc
    27481 gtcgcgcacc gcttcgtaca atcccacttt gaccttcgcc actttctcgc ccggcatatc
    27541 ggcaagtttg aggatcagat cgtccggatc gccattacac agcggtgccg cacggtagtt
    27601 ggctgcttgc ggcaacgtat ctgtcagttc tgccaatgca cagcttacgc caaaggccac
    27661 ggaaggcatc tgcggtagct cgcaatcgcc tgccagccag ttatttaccc aggcaagcag
    27721 cacgctttgc gcctcttccc agttttcctg gctgaagccc ggcagtgggg agatctcccc
    27781 ccacccttcg cgctcgccct cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg
    27841 cctgtcgcgc agaaccaccc ccgcgtccat ggggatctgc cagcggtata cctgcgcgct
    27901 acgcattacg gattccgttt aaatttgctg aaatcaggct gacgtttctg gttgaaagcg
    27961 ttgcgacctt cctgaccttc ttccgtcatg tagaacagca tggtggcgtt gcccgccagc
    28021 tcctgcagcc ccgcctgccc gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc
    28081 gccatcgggc tgttttgcag catttcgcgg caccaacgga cggtttcttt ttccagatcc
    28141 gccagcggta ccacggtgtt gacaaggccc atatccagcg cctgttttgc gtcgtactga
    28201 cggcacagga accagatttc tcgcgctttt ttctgcccga cgatacgagc catgtaggaa
    28261 gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa gatggcatta
    28321 tctgctgcga tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc gatggagtag
    28381 ccagccacca tcgcgacaac cggtttcgga caggtacgga tctgacgctg gaagtccagc
    28441 acgttcaggt gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc acgcactttc
    28501 tggtcaccac cggagcagaa cgctttatcg cctgcgccag tcagaatgat cacgccgata
    28561 ttgtcgtcat aacgcgcatc tgccagtgcc tggatcatct cttttaccgt cagaggacgg
    28621 aaggcattgc gcacctgcgg acgattaatg gtgatttttg cgataccgtc ggtggatttt
    28681 tcataacgaa tgtcctcgaa accttcggag cagtcgtgcc attcaaccgg tgcgtaaggc
    28741 attgcttcat caggataaat catattgtgt cctttagtca gaaacgcaag atctgcgcca
    28801 gacttgcgat tacgccagcg ggattttccc gatgcgcgtt atgtccggcg cgaggaatga
    28861 catggcagtc ggcagccagt tccgccgcca gggcgcggaa tttgctgtca cgttcaccac
    28921 ataaataata aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca ggctggacgg
    28981 cgagagaagt cgcctccagc atggcggcaa gggttgcgcc attattgttg ctgcgcagcg
    29041 ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt tgataccagt
    29101 cggcaaatac cgccgttaac ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac
    29161 gctgacgttc cgcacgttgt tcagcatttt gcagccccgg atgcccgcct tcgacaataa
    29221 ccccacaaag ccccgccagc ccctggcaag ccgccatcat cgccacccgt ccgccaagcg
    29281 agtaccccac cagccagaag tcaaggatgt tgtaactaac caaggtttta cgcagtaagt
    29341 cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccatga cctaggagat
    29401 caacatacaa ccgtgagtag tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat
    29461 cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tagttttccg tgttttgcct
    29521 gcgcgtgcag gatcataaat ggcttacctg cgccagaagt tgctggagcg tttgcgcacc
    29581 atcggtgtcg ttaaccacca tttcaatcac cgtggtggtt ggcgtgcgcc aggcgtcggc
    29641 aaatgccgtt tcaagttcct gccagttttg cggatgatga tatttcagct cgaacatcgc
    29701 ggcggcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcac gctcgctttg
    29761 cggcgttggc aacagcgaga aaatttgccc gccgttgttg ttcaccacaa ttaataccag
    29821 cggcgcagaa acctgacgca ataacgccag cgcgttgaga tcgtaaagcg cggagagatc
    29881 gcccacaatc gccagcgtcg gtttgccgct tgcccgctga acgcctgcgg cggtcgaaag
    29941 cagtccgtcg ataccgctgg cccctcggtt gctgtacacc gggtaacctg ccggaagttg
    30001 cgaaagcgca tcaatcagac gtaccaccag gctgttacca acaaacaatt gcccctgttc
    30061 aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat cacggcgggc
    30121 aataaccgcc tgcattgcct gttccgccag gcgcgggatt tcaacgcacc agggctggcg
    30181 tttttctgcc ggatgcagct ccagccagtc ggcaatattg gcaattaagc gacgtccgcg
    30241 atggtgtgcc ggatcaagtc gcccttcaat gtcatcaaca atccagtact cttctggttc
    30301 acagcttgcc tgccattgca ggagccgttt ccccgtcagg ctacttccca gttgcaccac
    30361 aatttgcgcc tgctgcagct cgctggtcgc tttggcattg cctaaccaaa gatcggcaca
    30421 cggcagcggc tgcccggttt gtgacagcac atcgccaatc agcggccagc caagagtttg
    30481 cgcccacagg gcaacttttt tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc
    30541 gcgcttttgt cgccagaaga accagtcgcg ctgtttttca ctttccagac gaggcgcttc
    30601 acgcagccac ggtttgtcgt cctgccacca gtcacccaga cgctgttgcc agctaagccc
    30661 ggtatcgtcc atttcgccat acagcggttc agcaaacggg cagttgatat ggactccccc
    30721 cgcatgaagc gtaccgagag cgtggtcgat ggtagaaacc agccaacgtg cggggatatc
    30781 ctgggtcggg cgcggcaatg aaatactgtg cgtggggtga gaggcgaaca ttcccggctg
    30841 gcgaattgcc tgattcgcgc cgcagtcaat tagctccggc gggcgatcgg cggttaagag
    30901 aatcagtttt tctccggtta acccggcttc aatcagtgcc ggatagagat ttgccaccgc
    30961 cgtgccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca gccccagcgc
    31021 cagatgcccc aacccacgct catcgaaatg ggtgtggtga atgaatgcgg aattctccgc
    31081 cgccgctaac gttaacggtg tagaacgcga gcctggggcg atacagatgt gtctgacgcc
    31141 gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg cgcttactga
    31201 catgagtttg tccggtatca atattgcggc taagtataag gagctacaaa aatagattat
    31261 tgatatgaat cggtaatgat gcgactcatt actattccat ttgtaataaa gtacgcagcc
    31321 ctgccgcttt gttgtcgatt tcctgccact cttgctcggg gtcggaacca cggacaatgc
    31381 ccgcgccagc atataatcgc acgacattgc cgctaatttt tgctgagcgc agggaaacgc
    31441 agaattcgct ttgttgtaat gagagatagc ccgcagaacc ggcgtaccat tctcgggtga
    31501 acggttcgtg acgggcgata aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg
    31561 ccgttggctg caactgatgt aaacagatca catcatccgc tttgttgagt gaagtccaga
    31621 tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt aaaacatcca
    31681 gcgtctgggt atcggcctgt aatcgttggc agatatcttc caccaccagc atgttctcgc
    31741 gctggttttt atcatccgcc atcagccact ctcctaactg ctgcgcctgc ttatcatcag
    31801 gatgatttgc tactgttccc gccagcgctt cagtacgcag cgctttgtca cgccgccgcc
    31861 ataaccgttc cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat
    31921 ggtagcaatt cagattcagt cgacgactgg cagccatcat cgccgccgcg ttgaccggac
    31981 ttgcgaaatg caggtcagtt gcccgagcga gcaccacttt gtcgagctca ccttcggcga
    32041 tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc cagtgttgtt
    32101 ctcgcgtggt ggttaaatgt aacccaggca agggcttgat actcaccagt gtggcgataa
    32161 attcttttgc ctgaatcgca tcgtgctgaa gggagctttc gctgaatagc gtcagccgca
    32221 gcgtggcttt accgccacag cgtcgccatt ccaggcgggg taaaagtaaa ttgccctgcg
    32281 acgggtcaaa agcattcagc ccccaaatgc gtaagtcagc gtgttccggg tgctggcgaa
    32341 gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg acggcagctt
    32401 cttcatcacc attacgttgt tgccagtaga attgcgggta cgtttgctga ctggccagcc
    32461 agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcgataacc cgaatgccgg
    32521 gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg
    32581 attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtact acaaaaaaaa
    32641 tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct agtaccagcc
    32701 cgacggccgc gcccacacca attccttgcc agggtttttc gtggacgtag tcatcagcac
    32761 gataaacagc ctgcttcgcc cgatagtaat aactgtctga agcctggcta acccgttttt
    32821 tcacatcatc cagcgctttt tctgcatgcg ctttcagctc aacatatttc tgatcggcgg
    32881 gatcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc aggtcgtcat
    32941 cgatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgcgt aaaacctgtc
    33001 actaactata gacaagagtt gttgattacg cctgaattac ctcgcgtgcc atcccaatgt
    33061 gtgggatacc atcttcttca tagacctccg tcacagggat aaaaccgaaa ctctggtaga
    33121 agttttgcag atgcgcctgc gccccgaggt aaacaggctt atcaggccag tgatgcgtac
    33181 atgtttcgag tgttttactc atcagctgct gccccacttt ttcaccacgc aacgcttcac
    33241 tgacaatcac ccgacctata acgaccggct caagatcatc atcacttttc agaatcctcg
    33301 catacgccac cagttcatca tttttccaac cgagaatatg gcgattatcc cccgtcaaat
    33361 catcaccatc gatatcctga taagggcaat tctgctcgac aacaaacacc gcgcaacgca
    33421 gttgtaataa ggcataaagc tgggaaaccg ataattcaga gtgatgcaga tcttgccatt
    33481 caatcataat gctctccttt tagatgtatg ccttatacta gccggaatat tttttgaaac
    33541 gtgatgaact catggaatta atttttttag gtacttcagc cggtgtgcca acccgcacgc
    33601 gcaatgtcac ggcaatattg cttaacctgc aacatccgac ccagagcgga ctttggttgt
    33661 ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac cctggaaaac
    33721 tggacaagat ttttatcagt caccttcatg gcgatcatct ttttggttta cccggcttgc
    33781 tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt ccgcaaggta
    33841 tccgtgaatt tgtggaaacc gcgctgcgga ttagcggctc atggaccgat tatccgctgg
    33901 aaattgtcga aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa gtaaccgctt
    33961 atccgctgga acacccactg gaatgttatg gctatcgtat tgaagaacat gataaaccgg
    34021 gtgcattaaa tgcccaggca ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg
    34081 aattaaaagc gggcaaaaca atcacgctgg aagatggaag gcagattaac ggcgcagatt
    34141 acttagctgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg
    34201 atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg acgctggata
    34261 taaccatgga agccaaagcc aatagtcgcg gccatagctc tacacgccag gctgcgacac
    34321 tagcccgtga ggctggagtc ggcaagctaa tcattaccca cgtcagctcg cgctatgatg
    34381 acaaaggttg tcaacacctg ttacgtgaat gcaggtcaat tttcccggcg actgaactgg
    34441 cgaatgattt caccgtgttt aacgtttaac tctgcttatt attaaaaaca gggtgaaact
    34501 tgccctgtta tagcaacccg cgctacatat atccacccac acaggcatat tgagcgattt
    34561 ttgatattta ttcatctcct tacgcactat attcagagca ataagttgcc tcttttcatc
    34621 ttgattcagc acaacctttg caactaaaag gagcccatga tggttacagt tgtcagcaat
    34681 tattgtcaat tatctcaaac ccaactcagt cagacatttg cagaaaaatt tactgtgacc
    34741 gaggaattac tgcagtcttt aaaaaaaaca gcgttatccg gagatgaaga aagtattgag
    34801 ttactgcata atattgcgtt aggttatgat gaatttggaa aaaaagctgg gtaatgctgc
    34861 caacttactg atttagtg
//
LOCUS       DAJTPG010000034        34445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTPG010000034 DAJTPG010000000
VERSION     DAJTPG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..332)
                     /locus_tag="OO230_001740"
     CDS             complement(<1..332)
                     /locus_tag="OO230_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691534.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OO230_001741"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OO230_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5691535.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OO230_001742"
     CDS             complement(1693..2073)
                     /locus_tag="OO230_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5691536.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OO230_001743"
     CDS             complement(2087..2503)
                     /locus_tag="OO230_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691537.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO230_001744"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OO230_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5691538.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OO230_001745"
     CDS             complement(3504..3971)
                     /locus_tag="OO230_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5691539.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OO230_001746"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OO230_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5691540.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OO230_001747"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OO230_001747"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5691541.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OO230_001748"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OO230_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5691542.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO230_001749"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OO230_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5691543.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OO230_001750"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OO230_001750"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5691544.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OO230_001751"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OO230_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5691545.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OO230_001752"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OO230_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5691546.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO230_001753"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OO230_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5691547.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OO230_001754"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OO230_001754"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5691548.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OO230_001755"
     CDS             complement(14626..15441)
                     /locus_tag="OO230_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5691549.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OO230_001756"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OO230_001756"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5691550.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OO230_001757"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OO230_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5691551.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OO230_001758"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OO230_001758"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5691552.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO230_001759"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OO230_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5691553.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OO230_001760"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OO230_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5691554.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OO230_001761"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OO230_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5691555.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OO230_001762"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OO230_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5691556.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OO230_001763"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OO230_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5691557.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OO230_001764"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OO230_001764"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5691558.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO230_001765"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OO230_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5691559.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OO230_001766"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OO230_001766"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5691560.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OO230_001767"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OO230_001767"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5691561.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO230_001768"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OO230_001768"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5691562.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO230_001769"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OO230_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5691563.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OO230_001770"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OO230_001770"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5691564.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OO230_001771"
     CDS             31575..32189
                     /locus_tag="OO230_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5691565.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OO230_001772"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OO230_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5691566.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OO230_001773"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OO230_001773"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5691567.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8699 c         9302 g         8336 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc cttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gtcacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcatcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 atttcctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgta acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgataatt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgtat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtaa tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgcag ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ttgtagcggg aaagcgtatt atgaataccc
    34441 cgcgc
//
LOCUS       DAJTPG010000035        33210 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTPG010000035 DAJTPG010000000
VERSION     DAJTPG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(49..141)
                     /locus_tag="OO230_001774"
     tRNA            complement(49..141)
                     /locus_tag="OO230_001774"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Ser,seq:gct)
     gene            complement(457..642)
                     /gene="csrA"
                     /locus_tag="OO230_001775"
     CDS             complement(457..642)
                     /gene="csrA"
                     /locus_tag="OO230_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5691568.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(877..3507)
                     /gene="alaS"
                     /locus_tag="OO230_001776"
     CDS             complement(877..3507)
                     /gene="alaS"
                     /locus_tag="OO230_001776"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5691569.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3635..4135)
                     /gene="recX"
                     /locus_tag="OO230_001777"
     CDS             complement(3635..4135)
                     /gene="recX"
                     /locus_tag="OO230_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5691570.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4378..5439)
                     /gene="recA"
                     /locus_tag="OO230_001778"
     CDS             complement(4378..5439)
                     /gene="recA"
                     /locus_tag="OO230_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5691571.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5519..6016)
                     /gene="pncC"
                     /locus_tag="OO230_001779"
     CDS             complement(5519..6016)
                     /gene="pncC"
                     /locus_tag="OO230_001779"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5691572.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6161..7246)
                     /gene="mltB"
                     /locus_tag="OO230_001780"
     CDS             complement(6161..7246)
                     /gene="mltB"
                     /locus_tag="OO230_001780"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5691573.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7502..8064
                     /gene="srlA"
                     /locus_tag="OO230_001781"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7502..8064
                     /gene="srlA"
                     /locus_tag="OO230_001781"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8061..9020
                     /gene="srlE"
                     /locus_tag="OO230_001782"
                     /gene_synonym="gutE"
     CDS             8061..9020
                     /gene="srlE"
                     /locus_tag="OO230_001782"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5691574.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9031..9402
                     /gene="srlB"
                     /locus_tag="OO230_001783"
                     /gene_synonym="gutB"
     CDS             9031..9402
                     /gene="srlB"
                     /locus_tag="OO230_001783"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5691575.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9406..10185
                     /gene="srlD"
                     /locus_tag="OO230_001784"
     CDS             9406..10185
                     /gene="srlD"
                     /locus_tag="OO230_001784"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5691576.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10290..10649
                     /gene="gutM"
                     /locus_tag="OO230_001785"
     CDS             10290..10649
                     /gene="gutM"
                     /locus_tag="OO230_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5691577.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10716..11489
                     /gene="srlR"
                     /locus_tag="OO230_001786"
                     /gene_synonym="gutR"
     CDS             10716..11489
                     /gene="srlR"
                     /locus_tag="OO230_001786"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5691578.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11482..12447
                     /gene="gutQ"
                     /locus_tag="OO230_001787"
     CDS             11482..12447
                     /gene="gutQ"
                     /locus_tag="OO230_001787"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5691579.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12444..13958)
                     /gene="norR"
                     /locus_tag="OO230_001788"
     CDS             complement(12444..13958)
                     /gene="norR"
                     /locus_tag="OO230_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5691580.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14145..15584
                     /gene="norV"
                     /locus_tag="OO230_001789"
     CDS             14145..15584
                     /gene="norV"
                     /locus_tag="OO230_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5691581.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15581..16714
                     /gene="norW"
                     /locus_tag="OO230_001790"
     CDS             15581..16714
                     /gene="norW"
                     /locus_tag="OO230_001790"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5691582.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16842..19094)
                     /gene="hypF"
                     /locus_tag="OO230_001791"
     CDS             complement(16842..19094)
                     /gene="hypF"
                     /locus_tag="OO230_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5691583.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19247..19774)
                     /gene="hydN"
                     /locus_tag="OO230_001792"
     CDS             complement(19247..19774)
                     /gene="hydN"
                     /locus_tag="OO230_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5691584.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19923..20933)
                     /gene="ascG"
                     /locus_tag="OO230_001793"
     CDS             complement(19923..20933)
                     /gene="ascG"
                     /locus_tag="OO230_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5691585.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20947..21066)
                     /locus_tag="OO230_001794"
     CDS             complement(20947..21066)
                     /locus_tag="OO230_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5691586.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21076..22785)
                     /gene="hycE"
                     /locus_tag="OO230_001795"
     CDS             complement(21076..22785)
                     /gene="hycE"
                     /locus_tag="OO230_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5691587.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22803..23726)
                     /gene="hycD"
                     /locus_tag="OO230_001796"
     CDS             complement(22803..23726)
                     /gene="hycD"
                     /locus_tag="OO230_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5691588.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23729..25570)
                     /locus_tag="OO230_001797"
     CDS             complement(23729..25570)
                     /locus_tag="OO230_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5691589.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHL
                     LNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNG
                     FAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRT
                     KEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMI
                     TLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFA
                     PVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25607..26218)
                     /gene="hycB"
                     /locus_tag="OO230_001798"
     CDS             complement(25607..26218)
                     /gene="hycB"
                     /locus_tag="OO230_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5691590.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26343..26804)
                     /gene="hycA"
                     /locus_tag="OO230_001799"
     CDS             complement(26343..26804)
                     /gene="hycA"
                     /locus_tag="OO230_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5691591.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            27016..27366
                     /gene="hypA"
                     /locus_tag="OO230_001800"
     CDS             27016..27366
                     /gene="hypA"
                     /locus_tag="OO230_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5691592.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27370..28242
                     /gene="hypB"
                     /locus_tag="OO230_001801"
     CDS             27370..28242
                     /gene="hypB"
                     /locus_tag="OO230_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5691593.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28233..28505
                     /gene="hypC"
                     /locus_tag="OO230_001802"
     CDS             28233..28505
                     /gene="hypC"
                     /locus_tag="OO230_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5691594.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28505..29626
                     /gene="hypD"
                     /locus_tag="OO230_001803"
     CDS             28505..29626
                     /gene="hypD"
                     /locus_tag="OO230_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5691595.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29623..30633
                     /gene="hypE"
                     /locus_tag="OO230_001804"
     CDS             29623..30633
                     /gene="hypE"
                     /locus_tag="OO230_001804"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5691596.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30707..32785
                     /gene="flhA"
                     /locus_tag="OO230_001805"
     CDS             30707..32785
                     /gene="flhA"
                     /locus_tag="OO230_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5691597.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32822..33175)
                     /locus_tag="OO230_001806"
     CDS             complement(32822..33175)
                     /locus_tag="OO230_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5691598.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7821 a         9269 c         8643 g         7477 t
ORIGIN      
        1 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg gcggtgaggc
       61 ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg ggagcgcctt
      121 cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg
      181 ctcatcatcg ctgcgtggcg cacatattac tttctgagac ttataagtca aacaattttt
      241 cccacacttt tatcgtttgc acacttcacg ctcaattagt ctgtaaaagc gtcaaaaaga
      301 gtgttttatc tacagaaaaa cggaggtctg gcagatagca gtaatgcaaa aaaatggaga
      361 cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag aaattttgag
      421 ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact gctgggattt
      481 ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct tcggggcatt
      541 tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga cctcatcccc
      601 aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct ccttgaaaga
      661 ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc caaaaagtaa
      721 gcaatgacaa acacattaca tgtaagcagt catggcatta cattctgtta aacctaagtt
      781 tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta atgcgaatgc
      841 cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg cgctgaccca
      901 gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc
      961 catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac caatcagttc
     1021 ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag aaaccttacc
     1081 ttcggctacc gttgccagca cgataattgt cgaccccagc tgatttttta aatcgtcaac
     1141 catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca gcttaacacc
     1201 attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt
     1261 aagctgttgt agttcttttt ctaactgacg cgtacgttcc agtactgagc gcactttatc
     1321 cgccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc gatcgctgtc
     1381 tgcatgaacg gtggtgatag cgccttctcc ggttaccgct tcgatacgac gaacgcctgc
     1441 agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg
     1501 agtaccgcca cacagctcgg tggagaaatc gcccatgctc agcacgcgta cacgctcatc
     1561 atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga gattcatgat
     1621 gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt cgaccgcacg
     1681 aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt
     1741 aaccagtgaa cctttctgcg atacatgagt ccccagaacc tggcgcagcg cagcgtgcat
     1801 caggtgcgtt gcggagtgat tcagacgaat acgggcgcga cgagcctcat caatatcagc
     1861 ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc
     1921 ctggccgtat ttctgcgtat cttccaccgc gaaggagaag ttagcgcctt tcagttcgcc
     1981 tttatcacca acctgaccgc cggattccgc atagaatggc gtttgatcca gcacgaccac
     2041 agcttcctgg ccggcattga tggcatcaac cgctttacca tcaacaaaca gcgcggtcac
     2101 tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactat caacacggat
     2161 cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcgcgacgac gctgctcttc
     2221 cattgctgct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac aaacatcagc
     2281 cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagccgttt caccatccag
     2341 cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc gctccagagt
     2401 acgggcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct gctggcgttt
     2461 caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa
     2521 ggtttctttc gcgccgagca tattgccgtg acgcactgcg cgacgaatga tacgacgcag
     2581 tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga acgcacaaga
     2641 acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggtcg cgccagtgac
     2701 tttcgctacc gcctggatca acgtgcggaa caggtcgatg tcatagttag agttaacgtg
     2761 ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg gcttcggcag
     2821 cggttccatc gtgccatctg cctggcggtt gaactgcatg aagacgatgt tccagatctc
     2881 aatgtagcgg tcgccgtctt cttccggact tcccggaggg ccaccccaaa tgtggtcgcc
     2941 gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca tctgccagaa
     3001 gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac gttcgcgcgg
     3061 gatcccgact tctttttccc agatttcgta ggcttcgtcg tcgctttcat agacggtaac
     3121 ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa
     3181 ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa
     3241 ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg
     3301 cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc caaggaacac
     3361 atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt tatggggtac
     3421 cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg
     3481 acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac gaaatatcgc
     3541 caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga ataagataag
     3601 ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt cgccaaatat
     3661 cctggatatc ttccatcaga tagccacgat agagcagaaa acgctggatc ttaacttttt
     3721 ctgaaaagac agttggcaaa ggttcaccat attttcgcgt cgcctgatcg cgcgccagtg
     3781 cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt
     3841 tctgattcag ttcctgacga atacgtgcag gtccataccc tttgcggcta cggctggcga
     3901 taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac caggcaataa
     3961 cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg cccataatcg
     4021 gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc agaatgcgta
     4081 ccgcacgatc caacaggcga gcatatgccg ggcgacggga tgttgattct gtcatggcat
     4141 atccttacaa cttaaaaaag caaaagggcc gcagatgcgc cccaggtttt gctgataaac
     4201 agatttaata gtcaatgagt atgcagaatg ttcccgcagg cccaattcgt tagaggatcg
     4261 aggtgagagt aatttttcac gccagtgctg gcctgttccc ctcaccctaa ccctctcccc
     4321 aaaggggcga ggggaccgtc cgtgcacaca ttatctatta tcatcaataa cgaacgatta
     4381 aaaatcttcg ttagtttctg ctactccttc gctgtcatct acagagaaat ccggcgttga
     4441 gttcgggttg ctcagcagca actcacgtac tttcttctcg atctctttcg cggtttccgg
     4501 gttatctttc agccaggcag ttgcattcgc tttaccctga ccgatcttct cacctttgta
     4561 gctgtaccac gcgcctgctt tctcgatcag cttctctttt acgcccaggt caaccagttc
     4621 gccgtagaag ttgatacctt cgccgtagag gatctgaaat tcagcctgtt taaacggtgc
     4681 agcgattttg ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc
     4741 tttcaccgcg ccgatacgac ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt
     4801 accaccggta gtggtttccg ggttaccgaa catcacacca attttcatac ggatctggtt
     4861 gatgaagatc agcagcgtgt tggactgctt caggttaccc gccagcttac gcatcgcctg
     4921 gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt cgatttccgc
     4981 tttcggcgtc agtgccgcca cggagtcaac gacgataacg tctactgcgc cagaacgcgc
     5041 cagggcgtca cagatttcca gtgcctgctc gccggtatcc ggctgggagc acagcaggtt
     5101 atcgatatcg acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat
     5161 aaacgcacag gttttacctt cacgctgcgc tgcggcgatc acctgcagcg tcagcgtggt
     5221 tttaccggaa gattccggtc cgtagatttc gacgatacgg cccatcggca gaccacctgc
     5281 cccaagcgcg atatccagtg aaagcgaacc ggtagagatg gtttccacat ccatggaacg
     5341 gtcttcaccc aggcgcatga tggagccttt accaaattgt ttctcaatct ggcccagtgc
     5401 tgccgccaac gctttctgtt tgttttcgtc gatagccatt tttactcctg tcatgccggg
     5461 taataccgga tagtcaatat gttctgttga aacaattata ctgtatgctc atacagtatc
     5521 aagtgttttg tagaaattgt tgccacaagg tctgcaatgc atacgcagta gcctgacgac
     5581 gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt aatgccttca ccgcgggcag
     5641 tggcaaaagc aaaccagacg gtgccgacag gcttctcttc actgccgcca tccggcccgg
     5701 cgataccact aatagacacg gcataatcag cacgagccgc tttcagtgca cctatcgcca
     5761 tttccaccac gacgggttca ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacgc
     5821 cgatcatctg cgctttggct tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg
     5881 agctaccggc aatatcggta atcactttcg ctacccaacc accggtacaa gactcggcag
     5941 ttgttacggt tgcgccacgg gctttcagcg cctgcccaac ctgttcgctt aactgcatca
     6001 gttcactgtc agtcatcaca acctctgtca gtcaaaaatt tatgccggac aagatagcac
     6061 tttcgtcagg aagatggtgt tggcgttgca attttacgag gaggattcag aaaaaagctg
     6121 attagccaga gggaagctca cgcccccctc ttgtaaatag ctactgtact cgcgccagcg
     6181 ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg ggtgctgtgg ttgtaacggg
     6241 tgatggtgta gaagttcggc agaccgtacc agtactggta gccggtgcca acatccagac
     6301 gcagcaggct ggcttgttga tggttgccca gcggctgctg tggcgttaaa cctgcggcgg
     6361 caagctgcga aatgctgtac ttagttttga aaccatttgg caagcctgga gcctgaccgt
     6421 ttgccattat cgcgacctga tcgcctttca cccagccgtg cgctttgaaa tagttcgcca
     6481 cgctaccgat cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta
     6541 ccgcatattg tttgtaagac gacggcataa actgtccgta gcccatcgcc ccggcaaagg
     6601 aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc catcagcagg aaggtttcca
     6661 gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat
     6721 cgaggatgcg agttttcccc atcacgcgcc cccagcgggt ttcaacgccg ataatcccga
     6781 cgataatttc cggcggtacg ccatacacct gccacgcgcg attcaacgca tcttcatact
     6841 gattccagaa aaccacaccg ttctgcacgt tgtccggcgt aataaatttt ttgcgataac
     6901 gcagccatgc gccgttcgga cctgatggcg gtttcaccga tgtggttggt gcctggttat
     6961 ccatcagccg cagtaccgaa tccagacgct tcgcctggga gagaatttcc tgcaactgct
     7021 gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa ctgctgggca ttcgggttat
     7081 tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag
     7141 acggcgttcc ggtggtcgtt tcagtctcag taggttttgg cttgctgcta caggcggcaa
     7201 gcaacacaaa aaggggaagc aatgttacat aacgacgctt gaacatgagg ggtccattta
     7261 acagattcaa ccaggggcaa gtatggtaaa gcatcacacc ccgcacaagg aagcggtagt
     7321 cactgcccga tacggacttt acataactca actcattccc ctcgctatac ttttattcaa
     7381 actttcaaat taaaatattt atctttcatt ttacgatcaa aataacactt ttaaaccttt
     7441 caacctgatt agattaggtt gccgtttggt aataaaacaa taaatcctga aggagagaac
     7501 aatgatagaa accattactc atggtgcaga gtggtttatc gggctgttcc aaaagggcgg
     7561 agaggtgttt accgggatgg tgaccggcat tcttccgctg ttgattagcc tgcaggttat
     7621 catgaacgca ctgattaatt ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg
     7681 cgccggtaac cctgtttccc gttacctact gttaccgtgc attggcacgt ttgtcttttg
     7741 caatccgatg accttaagcc tgggtcgctt tatgccggaa aagtacaaac ccagctacta
     7801 cgcggcggcc tcttatagct gccactcaat gaatggcctc ttcccccata tcaaccctgc
     7861 gaactgtttg tttatcttgg cattgccagc ggtctgacaa cgctgaacct gccacttggc
     7921 ccactggcgg tgagttatct gctggttggt ctggtcacca atttcttccg cggctgggtg
     7981 accgatctga ccaccgccat ttttgagaaa aagatgggca ttcaacttga acaaaaagtt
     8041 cacctggcag gagcaacatc atgacgcgta ttcggatcga aaaaggaacg ggtggctggg
     8101 gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat cgtctatatc accgccggta
     8161 cccggcctgc gattgttgac aaactggcac aacttactgg ctggcaggct attgacggat
     8221 ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac tgtggcggca
     8281 cattacgctg cggcatctat ccgaaacgac gtattcccac cattaatatc cactcgacgg
     8341 gcaagtccgg tccgctggcg cagtacattg tggaagatat ttatgtctct ggcgtaaaag
     8401 aagaaaacat cactgtagtg ggtgatgcga caccacaacc ctcttccgtg ggccgtgact
     8461 atgacaccag taagaaaatc accgaacaaa gcgatggttt actggcgaag gtgggaatgg
     8521 gcatggggtc cgccgttgcc gtgttgtttc agtctggtcg tgacaccatc gacactgtat
     8581 taaaaaccat tctgccgttt atggcattcg tctcggcgct cattggcatc attatggctt
     8641 ctggccttgg tgactggatt gcccacggtc ttgctccgct agcgagccat ccactgggtc
     8701 tggtcatgct ggcgctcatc tgctccttcc cactgctttc acctttcctc ggcccaggcg
     8761 cagttatcgc acaggttatc ggcgtattga ttggcgtgca gattggtctc ggcaatattc
     8821 cgccgcatct ggctttaccg gcactgtttg ccatcaacgc gcaggcggct tgcgacttca
     8881 tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac ggttcgcgtc ggtgtccctt
     8941 ctgtactggt gagccgcttt ttaaccggcg cgccgactgt actgatcgcc tggtttgtct
     9001 ccggttttat ctatcaatag aggctgaaac atgaccgtta tttatcagac caccatcacc
     9061 cgtatcggcg cgagtgccac tgacgccctc agcgaccaga tgctcatcac ctttcgtgaa
     9121 ggcgcgccag cggacctcga agagtattgc ttcattcatt gccacggcga gttgaaaggt
     9181 gcactccatc ccggtttgca attttcactc gggcagcatc gctatccggt gaccgctgtt
     9241 ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg cttcgatggt
     9301 ttaaacgaag cggaatttcc gggcactgtc catgtggcag gccctgtacc cgacgatatc
     9361 gcgccgggat cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa tcaggttgcc
     9421 gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt gccacggtct ggctgccgag
     9481 gggtatcgcg tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt ggcacaagaa
     9541 attaacgccg aatatggtga aggtatggcg tacggttttg gtgctgacgc cactagcgag
     9601 caaagcgttc tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt ggatttgctg
     9661 gtctatagcg ccggaatagc aaaagcagcc tttatcagcg acttccagct cggcgatttt
     9721 gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt gtgcccgtga attttcgcgt
     9781 ttgatgatcc gcgacgggat tcagggtcgc attattcaga tcaactcgaa atccggcaaa
     9841 gtgggcagca aacacaactc tggctacagc gcagcgaaat ttggtggcgt cgggctgact
     9901 caatcactgg cgctggatct ggcggagtac ggcattacgg tgcattcact gatgctcggt
     9961 aacctgctga aatcgcggat gttccagtca ctgttgccac aatacgcgac caagctgggt
    10021 atcaaaccgg atcaagtcga gcagtattac atcgacaaag taccgctcaa acgcggctgc
    10081 gattatcaag atgtgctgaa tatgctgctg ttctacgcca gtcctaaggc gtcgtactgc
    10141 accggacagt cgatcaatgt caccggcggt caggtgatgt tctgatcaac agcggagatc
    10201 cgttaaggat ctccgtgaga ctatagaatg cctgatgcgc tacgctcatc aggcatacag
    10261 gacttccacc actacattaa ggaaaagtta tggtatccgc actcatcacc gtcgccgtta
    10321 tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat ttctcgtttt aaccgtgcct
    10381 tcgacacact atgccagcaa gggcgggttg gcgtggaccg ttccagcggg cgctttaaac
    10441 cgcgggtcgt ggtcgccatc gcgctggacg atcagcagtg catcgtcgac accttgttta
    10501 tgaaaggact gactgtcttc gcccgaccgc aaaaaattcc cgcaattacc ggcatgcatg
    10561 cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag aatgctctat
    10621 cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg cttgcgaagt
    10681 tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag cgtcaggccg
    10741 ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg gcgcaatact
    10801 ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat gccggaaccg
    10861 tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg cctatcgatc
    10921 ataaaacgct catcaacacc cacaagaaag agctgattgc agaagctgcc gttagtttta
    10981 tccatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag attgttcccc
    11041 tgctctcgcg ctttaataac atcacggtga tgaccaacag cctgcatatc gtcaatgcgc
    11101 tatccgaact ggataacgaa caaactatcc tgatgccagg cggaacattt cgcaaaaaat
    11161 cggcctcatt tcacgggcaa ctggcagaga atgccttcga gcatttcacc ttcgataaat
    11221 tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccacgttc aacgaggttt
    11281 ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg gcggactcat
    11341 caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc gataagctaa
    11401 ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct ggaagagaaa gggatcgatg
    11461 tgatcataac cggagagagc aatgagtgaa gcactactga acacgggacg tcagacgtta
    11521 atgctggagt tgcaggaagc aagccgttta ccggaacgtc tgggcgatga ttttgttcgc
    11581 gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg
    11641 ggccacattg gtaagaaaat cgccgcaaca cttgccagta ccggcacacc ggcttttttt
    11701 gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg cgatgtgatg
    11761 ctgtttatct cttactccgg tggcgcgaag gaactggatc tgattattcc gcgtctggaa
    11821 gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct gggcctggcg
    11881 gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg
    11941 acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc ggtcatgcag
    12001 gcgcgcggtt ttaatgaaga agattttgcc cgctcccacc cagctggggc actgggcgct
    12061 cgcttgctga ataaagtgca tcatctgatg cgtcgtgatg acgctatccc acaggtagcg
    12121 ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc gcaccggtct ggggctggtg
    12181 gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta ccgacggcga tttacgtcgc
    12241 tggctggtag gcggcggcgc actcaccacg ccagttaatg aagcgatgac gacaggcggc
    12301 accacattac aggcacaaag tcgcgccatc gacgccaaag aagttctgat gaaacgcaaa
    12361 atcactgccg caccagtggt agatgaaaac ggcaaactca ccggcgcaat taacctgcag
    12421 gatttctatc aggccgggat tatttaatcc ttcaatccca gacgtttcgc cagccgatgc
    12481 aggttggcga cgtcagtttc cagcattcgc gcacaggctg cccagttgtg atgattttgt
    12541 gccagtgcct ggcgaatagt ttcacgctgg aacgcttctg tcgcttcacg caggttttgc
    12601 ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa
    12661 tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg ctctcgccag cactaccgcc
    12721 cgatgaatag catgttccag ttcgcgcacg tttcccggaa aactgtagtg ttgcagtaaa
    12781 tttcgcgccc cggcacttaa taccacgcgg gagagcccct gccgcaaacg acactgctcg
    12841 cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg cggcaccgaa
    12901 agtggaaaca cgctcaggcg atgaaacaaa tcggcgcgga atcgccctgc cagcacctct
    12961 tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat cgacccgcaa actacggtca
    13021 tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc ctgcaatgcc
    13081 aacgacaact cgccgatctc atcgagaaac agagtaccgt tatccgccat ttcaaacttc
    13141 ccgctgcggt tactgatagc gccagtaaac gctcctttca catgcccgaa caactcactt
    13201 tccgccacac tttccggcag tgcggcacag ttgagataga ccagcggatt caccgcccgt
    13261 ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt ctcaccgctg
    13321 atcagaacgt tgagatcgga cgccgccaca atctcaatct cttttttcag ttgcgtcatg
    13381 ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt caaacggcgt ggcatcgcct
    13441 ggcagcatat tctggctttc cagttgttca atcagcaacg cattgcttaa cgctcccgcc
    13501 gccagcgcgg caatcagccg taactcttcg tcgctgaaaa catcgaactg atcgggctgc
    13561 atcccgtcga gcgtcagtgc gccgatcagg ttttgtccga caaacaatgg cagaccaacg
    13621 caggcgtgaa ccttcagact ctcctgccca ggaatcaaac cgtcataggg atcgggcaat
    13681 tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg caatcgcttc cagccgtgga
    13741 tgcccttcca gcgcaaagcg tctaccgagt acatcctttg ccagaccgtc gatggcaagc
    13801 ggaataaact gccgcgaatc gtaacgtagc aacgcagacg catcgcactc cagcacctga
    13861 cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg ctgcaattcg
    13921 atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact gtcaatttga
    13981 ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact cattaatgag
    14041 cataatttta tttatagagt aaaaacaatc agataagaaa ctggcacgca atctgcaatt
    14101 agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct attgtggtga
    14161 aaaataacat tcattgggtg ggtcaacgtg actgggaagt gcgtgatttt cacggcacgg
    14221 aatataaaac gctgcgcggc agcagctaca acagctacct catccgcgaa gaaaaaaacg
    14281 tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg
    14341 aaatcgatct ggcggatatc gattacatcg tgattaacca tgcagaagag gaccacgccg
    14401 gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc gatctactgt actgccaacg
    14461 ctatcgactc gataaatggt catcaccatc atccggagtg gaattttaat gtggtgaaaa
    14521 ctggcgacac gctggatatc ggcaacggca aacagctcat ttttgtcgaa acaccaatgc
    14581 tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtgctg ttcagtaacg
    14641 atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg gatcagacgg
    14701 agcttttcga gcagtgccag cgttactacg ccaatatcct gacgccgttc agccgcctgg
    14761 taacgccgaa aattaccgag atcctgggct ttaacttacc agtcgatatg atagcgacct
    14821 cccacggcgt ggtctggcgc gataacccga cgcaaattgt cgagctgtac ctgaagtggg
    14881 ccgctgatta tcaggaagac agaatcacca ttttctacga caccatgtcg aataacaccc
    14941 gcatgatggc tgacgctatc gcccagggaa ttgcggaaac cgacccacgc gtggcggtga
    15001 aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa
    15061 aaggcgtgct ggtcggcact tcgacgatga ataacgtgat gatgccgaaa atcgccgggc
    15121 tggtggagga gatgaccgga ttacgcttcc gtaacaaacg cgccagtgct ttcggatctc
    15181 acggctggag cggcggtgcg atggatcgcc tttccacgcg cctgcaggat gcgggtttcg
    15241 aaatgtcgct cagcctgaaa gccaaatggc gaccagacca ggacgctctg gagttatgcc
    15301 gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg cagagcacgg
    15361 tgaatacggt agttaaagaa gaaacctctg ccacgacgac ggctgacctc ggcccacgaa
    15421 tgcagtgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca atgcaggacg
    15481 ttgcgccagg aacgccgtgg agtgaagtcc cggataactt cctctgcccg gaatgctccc
    15541 tcggcaaaga cgtctttgac gaactagcat cggaggcaaa atgagcaacg gcattgtgat
    15601 catcggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac aggacgccac
    15661 tattccatta accctgattg ccgccgacag catagatgag tacaacaaac ccgacctcag
    15721 ccatgttatc agtcaggggc aacgtgccga cgaccttacc cgccagacgg cgggtgaatt
    15781 tgccgggcag tttaatctgc gcctgtttcc gcacacctgg gtgacggata tcgatgccga
    15841 agcccatgtg gtgaaaagcc agaataatca gtggcaatac gacaagttag tgctggcaac
    15901 cggtgccagc gcctttgtcc cgcctgtgcc tgggcgtgag ttaatgctga cgttaaatag
    15961 tcagcaagag tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg tgttgattgt
    16021 cggcggtggt ttgattggta gcgaactggc gatggatttt tgtcgtgcag gcaaagcggt
    16081 cacgctaatc gacaacgctg ccagtattct ggcgtcatta atgccaccag aagtaagcag
    16141 ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttgaaat ctcagttgca
    16201 ggggctggaa aaaacggatt ctggcattct ggcaacgctg gaccaccagc gtagtatcga
    16261 agtggatgcg gtaattgccg ccaccggact gcgcccggaa acagccctgg cacgacgcgc
    16321 cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca gtaatgccga
    16381 tatttacgcg ctgggcgatt gcgcggaaat taacggtcag gtattgccgt tcctgcagcc
    16441 gattcaactt agcgggatgg tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa
    16501 actaccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc tggcaggcga
    16561 aacccagcgt caggatttac gctggcagat taataccgaa cgccagggaa tggtggcgcg
    16621 cggcgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga
    16681 ggcatttgta ttgttgaaaa cgttgccggt gtaggtgggc tactgtgcct aaaatgtcgg
    16741 atgcgacgct ggcgcgtctt atccgaccta cggggacgca tgtgtaggcc ggataaggca
    16801 tttacgccgc atccggcaag ggtgtccaaa tgcaacacgc tttatccgtt ctggacttca
    16861 cccgctaacc aacgcgccgc agcaataacc ccctgcccca gagacaaacc gccatcaccc
    16921 gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga gataatgcgc cagacgtgca
    16981 cgcagcaaac ggttatgaat aaccccgccg ctaaatacca gcgtagtgat accacgcatc
    17041 gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca gcgcatcatg aaacgcccac
    17101 gcgcgttgat taaccagtgc ctgccagttc agccactgct gccagaaagt ggcgagatcc
    17161 agttgattgt ccacccgcgg catcgtcacc ggatgcgtca ctccgtcgca tgaggctgcg
    17221 agcgcctcca gagcacaagc cgcttcacct tcataactta acgtggctgg cgcacagccc
    17281 agtgccgccg ccactgcatc gaacaaccgc ccacacgatg atgccagcgg cgcgttaatt
    17341 ccacgctcaa tggcccgcgc cagtacgctc cagttttgct gttgcacact tgccgtttca
    17401 gggtaattct gccactccgg cacaaagcgc aggcactgcg ccagcaggtt tcgccacggc
    17461 tgcttcgccg ccaaatcgcc acccggaagc gccactgcag gcaagccgcc caggtgctca
    17521 cattcgcgat agttcacccg caggcactcg ccgccccaca aagcgccatt ctcccccata
    17581 ccgataccgt cgagcgtcaa agcaatgaca tcaccgccat ccagcggcca ctgatgctct
    17641 gccagacacg ccgctgcatg ggcatgatga tgcagtaccg tttgcgtcgg cagattcatt
    17701 tcgctcgccc actggctgga gacatagccc ggatgcacat catgcacaat gtattgcgga
    17761 gtgaaatcgt agatgttttg catcaggcgt aacgcttcgc gccactgcat ctggatgcca
    17821 tcgtcactta aatcgcccag atgctgactc aacaccgctt gttcaccgcg caccaggcag
    17881 aaggtatttt tcagatccgc gccaagacac agcacaggcg gaacattttt aaagcccgga
    17941 ggcaaaacca gcgcatccgg cacatacccc cgcgaacggc gcagcatttc gccgctttcg
    18001 cgcaccactg aatcatccat ccgctgcacg atgtcgcggt tatgtagcaa gaatccgtcg
    18061 gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga tagctggtgg tttaccgctc
    18121 aggttgccgg aggtcatcac cagcgggcat tgcagttcct gtaacagcaa atgctggagc
    18181 gggttcgcag gcaacatcac cccgacttcg ttaaggccag gggcgatatc atcacaaagc
    18241 tcaggtacgt attttttatc caccagcaca atcggcgcag cgggcgtggt aagcaactgg
    18301 cgcgcagcgt ctggtaaact ttctgccact ggcaacatga ctgccagcgg tttcgccggg
    18361 cgatgtttgc gctcccgcag tgtcgccacc gcgttactgt tacgtgcatc gcaggcaaga
    18421 tgaaatccgc caatcccttt gatggcgaca atgttgccca tttttaactg tgcgatagct
    18481 gcctgtaatg ccgcctcttg ttctgcatgt tcaccatgac ttacccattc aagatgcggg
    18541 ccacactccg ggcaggccac cggctgggcg tggaagcgac gatcgagcgg gtcgcggtac
    18601 tctttgtcac aggccggaca tagcggaaac gccgccatca cggtaaacgg gcggtcgtaa
    18661 ggcatcgcgc gaataatggt gaaacgcggg ccgcagtggg tacagttgat aaacggataa
    18721 cgataacgcc gctcgcctgg ggtattcatt tcggcaaggc aagcagggca agtggcggca
    18781 tccgggacaa tttgcgtatt catggtgccg ccggtgctct ggcggatggt gaactcggtg
    18841 ggcagttgtg accagataaa cggctcacgc tcgacgctat caatacgcgc cagtggcgga
    18901 cagtgctgat gcaattgaac aagaaacgtt tccgggtctt ccagcagccg gacttctacg
    18961 ccatcgccgt cattacatac atcgccgtga agatttaatt gctgtgccag ctgccagaca
    19021 aacggacgaa aaccgacgcc ctgcactttg ccacgaatac gcagttggac accgcaagat
    19081 gtgttttttg ccattgagtt attcccgcca tcatgaattg cgtaacccgc cctgccggac
    19141 acgacagcgt cgtatccggc agtctcaggt cggcgatacc gcccgctccg tattctacga
    19201 atatttccgg gaattccttt gatgccagaa cagttctgta agatttttag aacatcagcg
    19261 ccgtacggcg gcgtttttct gcgctcagtt gttcaagttt attacgatcg acacaaatca
    19321 gcgcatgggt cgggcaagcc gccatacacg ccgggccgtc ttcacgatgg ttgcacaggt
    19381 cgcatttatt ggcttcggct ttgtcagccc gtacattcag acccgcgccg ctgttgcgaa
    19441 tcacaggacg taccaccact tccatcgcgc catacgggca agccaccacg caggttttgc
    19501 aaccaatgca acgttcctgc atcacaggaa caaacccttt atcacggctg atagcaccat
    19561 tcgggcagac gttagcgcac ggtgcatctt cacactgacg gcaaactgtc gccgtggaaa
    19621 tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt ttccggggtc agcgatgcac
    19681 agtcctgatt ttcctgatga gaaaccacgc acgccacttc acaggtacgg caaccaatac
    19741 atttactcgc gtcagcaatg atgaaacggt tcatcaaatt ctccagcaat gacagttaat
    19801 gcgccgatac actcacaaat catgccagtt tttaatttgc tgttatttaa ggaaattaat
    19861 ttctgtaatg caggaaaaaa gatgtcatcg acactagtga cgatgacatg tgatgacaat
    19921 gtttatcgcg aaagggcaat gagtgagtcg cggcggatca gttttccgct aaaggttttc
    19981 ggcggtgaga aatccccgcc atcgagcata aaaatcagcc gtccaataat ttcctgaatc
    20041 atctcagtca ccggaatttt tacgctggag agcgccggaa cgatataggg ggcaattgcg
    20101 atatcatcga atccgataac tgacacctgc tctggcaccg ctacgccgcg ctcgtgcaac
    20161 gctttgatcg cacctatcgc catatcgtcg ttactggcaa ctaacgcgct aaatttagcc
    20221 cctcgttcga gcaacgtttc taccccttcg gccccgctgg cgggcgtcca tttaccgtta
    20281 gcgataagtt tttcattgag cgcaatacca tgctgcgaca gcgcgtcttt atacccggca
    20341 agacgttcaa tgctggtggg ggaatccatc gagccggtaa ggaaagcaat ctcctgatgc
    20401 ccggcgttta tcaactctgc cacggcgtta aagctggtct gtttatgatc gcaccagacg
    20461 ctatggctgc tattattgcg caggcggcga ttaagcacca ttatcggctg actgtgcgcg
    20521 tcaatgatgt catcaatctc atccacgctt aaaaaacgcg ggtaaatcat aatggcgtcg
    20581 cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt cttcggcgct gtgtttacca
    20641 tctgccaata gcaactgtcg ccctttctct tccgccattc gcgcggcatg aaacagtaat
    20701 tcactaaaat aaatgccgtg ataaagcgtg ttggtcacta ccagccccag cgtctgagta
    20761 ctcttcgccg acagattgcg ggccagcaaa tttgggcggt aactgctctc ttctaccgcc
    20821 tgaaacacgc gatctttagt ctcctggctg acgtagccat tacctgaaag cacgcgggaa
    20881 acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca gcatcgtcgt catcattttc
    20941 atccctttac acgcaatcaa cgcagtgtac tgcaccgttt gccgattgtc cttgcgcaat
    21001 cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt
    21061 aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag
    21121 ttctttgtac ggcaccactt tgctcttctt cttacgcaca tcgactacgg tcatgcggtc
    21181 ggtacaggag tagcaagggt ccaggctacc gataatcagc ggcgcatagg aaacggtatt
    21241 gccacgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca
    21301 gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc
    21361 ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg
    21421 accgcccggc aggttatcca gaccgtagtc gatcatattc agcgcggtat agacctcgtt
    21481 gattcgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg
    21541 cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga gaccgctggc
    21601 gcgaaccatc gggccaacgt tactgaaatc gcgggcgatt tccgggtcca gacgaccaat
    21661 gccgacggtg cgttgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac
    21721 ttcacgacgc atttgttgcg ccagctggcg ggtctggatc atgtcatctt tcagcagatc
    21781 gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc
    21841 tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc
    21901 ggtaaagtga caggcgaggc cgaggttgag cagatgcggg tgcaggcgtt ccacctccag
    21961 cagaatggcg cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac
    22021 cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag
    22081 gaaggtcact tcgttataac ccatgcgggt ttccgccagt ttttccatac cgcgatggac
    22141 gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg
    22201 acccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc
    22261 gcccagttcg ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg
    22321 ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagcac
    22381 cagacgacgt tcatccggca gaccaaccgg aatcaaaccg tacatatcgc gcacttcgcg
    22441 ctcgccccac accgccgccg gaacgcgcgg cgtcacggac ggatactccg gtttgttggc
    22501 gtcaacttcg acgcgaaccg ttacccaaca cttggtgccc ttctccatcg acagcacgta
    22561 ataaacggcg taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca ccgacagcca
    22621 gccaccctgc ttgtagtaga gaaactccac cacttccggc agatagttca cctttacggt
    22681 gacagtcagc tgatctttgg tctgccaggc gtggtccagc acgacgccta gaaatgcctc
    22741 attcagcgcg gcgagataat gttgacctaa tttttcttca gacatgctca aactctcttt
    22801 aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc agcccaggta
    22861 atgcgcggag taatatcaag acgcagacgg gccatgctgt tttcgaacag cgcgataacc
    22921 aggacgccga ccaccagttt gacgatggca atcaccagcg ccagcagcag tccaccgacg
    22981 gtgaaggttt ccatttgtcc ccacggaata aacaccccga cgaacatctg caacaccacc
    23041 agctgtttca ggctgatacc ccatttcatg acgccaaagc cgctaccgct gtattcggag
    23101 agcggacctt cctgcaactc ctgctcggct tccgccaggt cgaacggcag tttgcccatt
    23161 tcgataaaag tggcgaacgc acaggcgcag agcgccagca ccagcgggat gctttggctc
    23221 agcggccagt gataaacggt gtcggtaatg ttgctgatgt tggtggaacc ggcaacctgt
    23281 gcggcaaccc acagaccaag cagcaacatc ggttcgacca gcacgccaag catcgcttca
    23341 cggctcgcac cgatagcggt aaacgggcta ccggtatcca gaccggaaat ggcaaagaag
    23401 aaacgcgcga tggcgaagag atacagtaag gtgatcaaat cgcccagttg cggcagcgga
    23461 gaaccgacgg tcaccaccgg cagcgcagta gcgatagtca gcatgacgcc caccatcaca
    23521 tacggcgtca ggcggaacat ccagccagag gcatccgggc caacgctctg gcggcccagc
    23581 agtttgataa tgtcgcgata ctcctgcaac acgcccggcc cgcgacggtt atgcagacgg
    23641 gcgcgcgcca cgcgggtaat accggagagc agcggcgcaa cggcaaataa caccagcgcc
    23701 tgaattaacg gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca
    23761 gtaccgccag ttcaaccagc gccatccggc ggaacagcaa cgcactacct tcgcactgcc
    23821 agcccggcac tagcgacacc ggattcagcc atttgcgcag tttcagcact ggcgcaaacg
    23881 cctgtttaac cggcatggca aaaccgtgag cggtaatcac cattgatttt tcatggtcgt
    23941 agccgcacac ccaggccgca ccgcgggaac gtgacggcag gcggtcgcct ttgcaaatcg
    24001 ccataatgat gaatggcagc agcgggcagg caatcagcag caatgtgatc attggttgag
    24061 aaacggtggt gttagcaggc tccagaggca gaggtacagc agcagagagc atcggcagca
    24121 gccacggcgc agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttacgc
    24181 tcatcaggag cggcgcacag gtggcgtttt cggcttcttt ggtacgcggc gcgccgagga
    24241 acgtgacgcc atagactttc gccatacaca tcaccgccag cgcaccggta attgccagac
    24301 ccacagcgag cagcggcccc agcagacggg caacaaacgc gccgctattg ctcagtttga
    24361 agaaggattg atagataacc cattccccgg caaacccgtt cagtggcggc agcgcagcca
    24421 ttgccatcaa cccgactaac atggcgatgg agataaccgg cattttcttg ccaataccac
    24481 cgagtttttc gatatcgcga tgaccggtac ggaaccagac gctccccgcc ccgaggaaca
    24541 gtacgctttt gaacaggcta tggttaagca gatggtacag accaccgacc aggccaagag
    24601 caatcagcgc cggttgttcg agcgcgatac ccgttacgcc agcgcccagc cccagcccca
    24661 gccccagccc cagccccagc cccagcccca gccccagccc cagccccagc cccagcagga
    24721 taatgccgat attttccagg gtgtggtaag ccagcaggcg ctggatgttg tgttccatca
    24781 acgcatacag accaccgacg aacgcggtga tcatgccgag caccagcagc gcgatccccc
    24841 accacagcgg tgcattaccg cccagcagtg acagggttaa catgcccagc aggccaattt
    24901 tcatgactac cgtagaaaac aacgcggcag ctggcgcaga ggcgttagca tgtgcctgcg
    24961 gcacccagcc gtgcagcgga ataatcccag ccagcaggcc aaagccaatc acaccgagca
    25021 gccagatatc ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca
    25081 gcgtgccata acgctgccac agcagccagc aagcaatcac cagcagcaga gtgccaagac
    25141 gcctcagcgc aaaccacagt ttgccctctt tgctgttgct ggtgaggaac accgcacaca
    25201 aggccatgac ttcggccatt accacgaaca tgccgaggtt gctggcgatg acggcgcaga
    25261 cggcggcagc catcaacata ttgatctgca agccgttgca cttcacctgc gcgtggcgat
    25321 gccagtcaat gttatagagg ctgacaaaca gaccgcaaag gccgagggta atcagccaaa
    25381 tcgcgttaag cggggagatt tgcacatcgt agcttaccag cgacagcgca ccgctcacgc
    25441 caaccgcgcc agtcagtaca gtgaagcccg cggctgccgt atacagacta ccaaccgcgc
    25501 cgccaattcc ggctatccag ccacttagcg ctttttgaaa agagaaatgc cagaacagcg
    25561 gcggcgacaa accatgccac gccgctattg atcagggaaa ttgcgctcat ttagcctctc
    25621 cactttgagc ctgctgaaac aaggtgagat cgccaaagtc cgtgttaaag gtcagctcac
    25681 gcttacgttt gctgacgcgg gcgatatcgg tgttatccac cagatgcagg gctttagtcg
    25741 ggcacatccg cacacaggcc ggaccttgtt catcaaagct acaaaggtca catttgacgg
    25801 cgatcgcgcg aatacctggc acccagtcaa gcaatgtgct gacacgcgcc ggagcaggcg
    25861 gtgccggtgg cgctttcggg gtattggcgt ttgccggaat atccagcgga cggctgccgg
    25921 aaaattcaat tgcgccaaac gggcaggcga tgccgcacag tttgcagctt acgcacagac
    25981 tttcattcaa ctgcacggcc ccatcaacgc gggtgatggc gttaaccggg cagaccaccg
    26041 cgcagggtgc atcttcacag tggtgacaga gctgcggcgc agattctttt tcattgagca
    26101 tcactctcag gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg
    26161 cctcacaggt gtggcagccg atacagagcg tggagtcggc aattacaaaa cgattcacca
    26221 ggcattcctc aggtgattgt catttttgac gaaaacatgc cgatgaaatg tcattttcga
    26281 cactcatcga cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg caaagcctca
    26341 gctcatgctg ccgggctttg tccctttacc agttggctta aattcaccgg cacattgttt
    26401 tcaatggcgg tgtataagct gtcgtaaacg ccagcgattt tttcgagata gtgttccaga
    26461 acctgttcgc gatcgcggat gttgaccttg ccatcaatca tgccgtcaat gctcagttgc
    26521 gcctgcgcaa tccgctgttt gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg
    26581 tggctgttaa agccatccgc cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa
    26641 cagagttctt tgtccggcgc gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg
    26701 atcccctgaa ccagcgaatt gcagtagatg tgccactggt cctgtaggcg acgatgccgc
    26761 tgtgcgatgt aatcggcttt ctcgcttatt tcccaaatag tcattgtcag gttactcgtt
    26821 taacagagat gccagcttta agcatttttt gtgccaactt ttaatttatt gttattaaag
    26881 agatttttaa gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg
    26941 acattattca ccgcagggat aatcaacact ggcgcaatta ttgcttgtag ctggcaatag
    27001 ttaatgggag gcgatatgca cgaaataacc ctctgccaac gggcactgga attgatcgaa
    27061 cagcaggccg caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa aattggcgca
    27121 ttttcttgtg tcgaaaccag ctctcttgcc ttttgttttg atctggtttg ccgcggcagc
    27181 gtggcggaag gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca
    27241 tgccaacagt atgtgacgct actgacccag cgcgtccgcc gctgtccaca gtgtcatggt
    27301 gacatgctac agattgtggc agacgacggt ttacagattc ggcggataga aatagaccag
    27361 gagtgagcga tgtgtacaac atgcggttgc ggtgaaggca acctgtatat cgagggtgat
    27421 gaacataacc ctcattccgc gtttcgtagc gcgccatttg ccccggcggc acgcccgcag
    27481 atgaaaatca ccggcattaa agcgcctgaa tttaccccca gccagactga agaaggcgac
    27541 ctgcattacg gtcatggcga agcgggcact cacacaccgg gcatgagcca gcgtcggatg
    27601 ctggaagtcg aaattgacgt gctggacaaa aacaaccgtc tggctgaacg caaccgcgcg
    27661 cgctttgctg cccgcaagca actggtgctc aacctggttt ccagccctgg ttccggtaaa
    27721 accaccctgc tgacggaaac cttaatgcgc ctgaaagaca gcgttccgtg cgcggttatt
    27781 gaaggcgacc agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg tacaccagcg
    27841 attcaggtga acaccggtaa aggctgccat cttgacgcac agatgattgc cgacgccgca
    27901 ccgcgtctgc cactggacga taacggtatt ctgtttatcg aaaacgttgg caacctcgta
    27961 tgcccggcca gcttcgatct cggtgaaaaa cacaaagtag cggtgctttc cgttaccgaa
    28021 ggtgaagaca aaccgctgaa atatccgcat atgtttgccg ccgcctcgct gatgctgctc
    28081 aacaaagttg acctgttgcc gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc
    28141 cgcgaagtca atccagaaat tgaaatcatc cttatttccg ccaccagcgg cgaagggatg
    28201 gaccagtggc tgaactggct ggagacacag cgatgtgcat aggcgttccc ggccagatcc
    28261 gcaccattga cggcaaccag gcgaaagtcg acgtctgcgg cattcagcgc gatgtcgatt
    28321 taacgttagt cggcagctgc gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg
    28381 ttcacgttgg ctttgccatg agcgtaatta atgaagccga agcacgcgac actctcgacg
    28441 ccttacaaaa catgtttgac gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa
    28501 aataatgcgt tttgttgatg aatatcgcgc gccggaacag gtgatgcagt taattgagca
    28561 tctgcgcgaa cgtgcttcac atctctctta caccgccgaa cgccctctgc ggattatgga
    28621 agtgtgtggc ggtcataccc acgccatttt taaattcggc ctcgaccagt tactgcctga
    28681 aaacgttgag tttatccacg gtccgggttg cccggtgtgc gtactgccga tgggtagaat
    28741 cgacacctgc gtggagattg ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc
    28801 catgcgcgta ccagggaaac agggatcgct gttgcaggca aaagcacgcg gtgccgatgt
    28861 gcgcatcgtt tactcgccga tggatgcgtt gaaactggcg caggagaatc caacccgcaa
    28921 agtggtgttc ttcggcttag gttttgaaac caccatgccg accaccgcca tcactctgca
    28981 acaggcgaaa gcgcgtgatg tgcagaattt ttacttcttc tgccagcaca ttacgcttat
    29041 cccgacgctg cgcagtttgc tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc
    29101 gggccacgtt agtatggtta tcggcactga tgcctataat tttatcgcca gcgattttca
    29161 tcgtccgctg gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg tggtcatgct
    29221 ggtggagcag aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc gggtggtacc
    29281 ggatgccggt aacctactgg cgcaacaggc gattgccgat gtgttctgtg tcaacggcga
    29341 cagcgaatgg cgcggcttag gcgtgattga atcttctggc gtgcacctga cgccggatta
    29401 tcaacgattc gatgccgaag cacatttccg cccggcaccg cagcaggtct gcgatgaccc
    29461 gcgcgcgcgt tgtggtgagg tattaacggg caaatgtaag ccgcatcaat gcccgctgtt
    29521 tggtaacacc tgtaatcctc aaaccgcatt tggtgcactg atggtctcct ccgaaggagc
    29581 gtgcgccgcg tggtatcagt atcgtcagca ggagagtgaa gcgtgaataa tatccaactc
    29641 gcccacggca gcggcggcca ggcgatgcag caattaatca acagcctgtt tatggaagcc
    29701 tttgccaacc cgtggctggc agagcaggaa gatcaggcac gtcttgatct ggcgcagctg
    29761 gtagcggaag gcgaccgtct ggcgttctcc accgacagtt acgtcattga cccgctgttc
    29821 tttcctggcg gtaatatcgg caagctggcg atttgcggca ccgcgaatga cgttgcgggc
    29881 agtggcgcta ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg attgccgatg
    29941 gagacactga aagccgtagt gaccagcatg gcagaaaccg cccgcacggc aggcattgcc
    30001 atcgttactg gcgatactaa agtggtgcag cgcggcgcgg cagataaact gtttatcaac
    30061 accgctggca tgggcgcaat tccggcgaat attcactggg gcgcacagac gctaaccgca
    30121 ggcgatgtat tgctggttag cggtacactc ggcgaccacg gggcgactat ccttaacctg
    30181 cgtgagcagc tggggctgga tggcgaactg gtcagcgact gcgcggtgct gacgccgctt
    30241 attcagacgc tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt
    30301 gtaaacgcgg tggttcatga gttcgcggca gcctgcggtt gtggtattga aatttctgaa
    30361 tcagcgctgc cggttaaacc tgccgtgcgc ggcgtttgcg aattgctggg actggacgcc
    30421 ctgaactttg ccaacgaagg caaactggtg atcgccgttg aacgcaacgc ggcagagcaa
    30481 gtgctggcag cgttacattc ccatccactg gggaaagacg cggcgctgat tggtgaagtg
    30541 gtggaacgta aaggtgttcg tcttgccggt ctgtatggcg tgaaacgaac cctcgattta
    30601 ccacacgccg aaccgcttcc gcgtatatgc taataaaatt ctaaatctcc tatagttagt
    30661 caatgacctt ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt catatacacc
    30721 gatgagtgat ctcggacagc aagggttgtt cgacatcact cggacactat tgcagcagcc
    30781 cgatctggcc tcgctgtgtg aggctctttc gcaactggta aagcgttctg cgctcgccga
    30841 caacgcggct attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt acgcgtcgcg
    30901 tgaaaaagac acccccatta aatatgaaga cgaaactgtt ctggcacacg gtccggtacg
    30961 cagcattttg tcgcgccctg atacgctgca ttgcagttac gaagaatttt gtgaaacctg
    31021 gccgcagctg gccgcaggtg ggctataccc caaatttggt cactattgcc tgatgccact
    31081 ggcggcggga gggcatattt ttggtggctg tgaatttatt cgttatgacg agcgtccctg
    31141 gagcgaaaaa gagttcaatc gtctgcaaac atttacgcag atcgtttctg tcgtcaccga
    31201 acaaatccag agccgcgtcg tcaacaatgt cgactatgag ttgttatgcc gggagcgcga
    31261 taacttccgc atcctggttg ccattaccaa cgcggtgctt tcccgcctgg atatggacga
    31321 actggtcagc gaagtcgcca aagaaatcca ttactatttc gacattgacg atatcagcat
    31381 tgtcttacgc agccaccgta aaaacaaact caacatctac tccactcact atcttgataa
    31441 acaacatccc gcccacgaac agagcgaagt cgatgaagcc ggaaccctca ccgaacgcgt
    31501 gttcaaaagt aaagagatgc tgctgatcaa tctctacgag cgggacgatt tagcccccta
    31561 tgaacgcatg ttgttcgaca cctggggcaa ccagattcaa accttgtgcc tgttaccgct
    31621 gatgtctggc gacaccatgc tgggcgtgct gaaactggcg caatgcgaag agaaagtgtt
    31681 taccactacc aatctgaatt tgctgcgcca gattgccgaa cgtgtggcaa tcgctgtcga
    31741 taacgccctc gcctatcagg aaatccatcg tctgaaagaa cggctggttg atgaaaacct
    31801 cgccctgacc gagcagctca acaatgttga tagtgaattt ggcgagatta ttggccgcag
    31861 cgaagccatg tacagcgtgc ttaaacaagt tgaaatggtg gcgcaaagtg acagtaccgt
    31921 gctgatcctc ggtgaaactg gctcgggtaa agagctgatt gcccgtgcga tccataatct
    31981 cagtgggcgt aataatcgcc gcatggtcaa aatgaactgc gcggcgatgc ctgccggatt
    32041 gctggaaagc gatctgtttg gtcatgagcg tggggctttt accggtgcca acgcccagcg
    32101 cattggtcgt tttgaactgg cggataaaag ctctctgttc ctcgacgaag tgggcgatat
    32161 gccactggag ttacagccga agttgctgcg tgtattgcag gaacaggagt ttgaacgtct
    32221 cggcagcaac aaaatcattc agacggacgt acgtttaatt gccgcgacta accgcgatct
    32281 gaaaaaaatg gtcgccgacc gagagttccg tagcgatctc tattaccgcc tgaacgtatt
    32341 cccgattcac ctgccgccac tacgcgagcg tccggaagat attccgctgc tggcgaaagc
    32401 ctttaccttc aaaattgccc gtcgtctggg gcgcaatatc gacagcattc ctgccgagac
    32461 gctgcgcacc ttgagcaaca tggagtggcc gggtaacgta cgcgaactgg aaaacgtcat
    32521 tgagcgcgca gtattgctaa cacgcggcaa cgtgctgcag ctgtcattgc cagatattgc
    32581 tttaccggag cctgaaacgc cgcctgccgc aacggttgtc gcccaggagg gcgaagatga
    32641 atatcagttg attgtgcgcg tgctgaaaga aaccaacagc gtggttgccg ggcctaaagg
    32701 cgctgcgcaa cgtctggggc tgaaacgcac gaccctgctg tcacggatga agcggctggg
    32761 aattgataaa tcggcattga tttaactgca aattgccgga cagatctgcc tgtccggcat
    32821 actattcacg aggttttttc ggacgatatt tttccggcag ttctggcacc ggacgcttgt
    32881 catcgatgag atgacgcacg gttaagatcg gatgacgcca cagcattctc ggcccggccc
    32941 aacgcataat ctgtttcatc tcttcacgct ttgcaggctg gtaacagtgc accggacact
    33001 gcttacaggc tggtttctct tcgccgaaca cacatttatc cagccgcttt tgcgcgtaaa
    33061 caaacaacgc ctcgtaatgc tccggctccg ctgacgcctg cgggcatttc gcttgataaa
    33121 gatcgatcat ttttttaatc gtcagttttt cacgagagat acgcttgccg gacatgctgc
    33181 ctccacctca ttaagatgca tttatattac
//
LOCUS       DAJTPG010000036        32912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTPG010000036 DAJTPG010000000
VERSION     DAJTPG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..2098
                     /locus_tag="OO230_001807"
     CDS             <1..2098
                     /locus_tag="OO230_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5691599.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="OO230_001808"
     CDS             2095..3183
                     /locus_tag="OO230_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5691600.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO230_001809"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="OO230_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5691601.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="OO230_001810"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="OO230_001810"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5691602.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="OO230_001811"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="OO230_001811"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5691603.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="OO230_001812"
     CDS             8051..8602
                     /locus_tag="OO230_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5691604.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO230_001813"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="OO230_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5691605.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO230_001814"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="OO230_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5691606.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="OO230_001815"
     CDS             complement(11683..12681)
                     /locus_tag="OO230_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5691607.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="OO230_001816"
     CDS             complement(12760..13452)
                     /locus_tag="OO230_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5691608.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="OO230_001817"
     CDS             complement(13529..14032)
                     /locus_tag="OO230_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5691609.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="OO230_001818"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="OO230_001818"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5691610.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="OO230_001819"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="OO230_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5691611.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="OO230_001820"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="OO230_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5691612.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO230_001821"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="OO230_001821"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5691613.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO230_001822"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="OO230_001822"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5691614.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21341)
                     /locus_tag="OO230_001823"
     CDS             complement(20922..21341)
                     /locus_tag="OO230_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691615.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21319..21672)
                     /locus_tag="OO230_001824"
     CDS             complement(21319..21672)
                     /locus_tag="OO230_001824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691616.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21673..21993)
                     /locus_tag="OO230_001825"
     CDS             complement(21673..21993)
                     /locus_tag="OO230_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691617.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22127..23560)
                     /locus_tag="OO230_001826"
     CDS             complement(22127..23560)
                     /locus_tag="OO230_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5691618.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="OO230_001827"
     CDS             complement(23623..24072)
                     /locus_tag="OO230_001827"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5691619.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO230_001828"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="OO230_001828"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5691620.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO230_001829"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="OO230_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5691621.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="OO230_001830"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="OO230_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO230_001831"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="OO230_001831"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5691622.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="OO230_001832"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="OO230_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..>32912)
                     /gene="nfeR"
                     /locus_tag="OO230_001833"
                     /gene_synonym="yqjI"
     CDS             complement(32383..>32912)
                     /gene="nfeR"
                     /locus_tag="OO230_001833"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5691623.1"
                     /translation="HGHQHEHGQCCGGRHGRGGGRRQRFFGHGELRLVILDILSRDDS
                     HGYELIKAIENLTQGNYTPSPGVIYPTLDFLQEQSLITIREEEGGKKQIALTEQGAQW
                     LEENREQVEMIEERIKARCVGAALRQNPQMKRALDNFKAVLDLRVNQSDISDAQIKKI
                     IAVIDRAAFDITQLD"
BASE COUNT         8037 a         8482 c         8650 g         7743 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgct tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tc
//
LOCUS       DAJTPG010000037        31169 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTPG010000037 DAJTPG010000000
VERSION     DAJTPG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            383..2041
                     /locus_tag="OO230_001834"
     CDS             383..2041
                     /locus_tag="OO230_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5691624.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2038..2994)
                     /locus_tag="OO230_001835"
     CDS             complement(2038..2994)
                     /locus_tag="OO230_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5691625.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3145..4260
                     /gene="macA"
                     /locus_tag="OO230_001836"
     CDS             3145..4260
                     /gene="macA"
                     /locus_tag="OO230_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5691626.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4257..6203
                     /gene="macB"
                     /locus_tag="OO230_001837"
     CDS             4257..6203
                     /gene="macB"
                     /locus_tag="OO230_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5691627.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6276..6500)
                     /gene="cspD"
                     /locus_tag="OO230_001838"
     CDS             complement(6276..6500)
                     /gene="cspD"
                     /locus_tag="OO230_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5691628.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6823..7143
                     /gene="clpS"
                     /locus_tag="OO230_001839"
     CDS             6823..7143
                     /gene="clpS"
                     /locus_tag="OO230_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5691629.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7174..9450
                     /gene="clpA"
                     /locus_tag="OO230_001840"
     CDS             7174..9450
                     /gene="clpA"
                     /locus_tag="OO230_001840"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5691630.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9853..9940)
                     /locus_tag="OO230_001841"
     tRNA            complement(9853..9940)
                     /locus_tag="OO230_001841"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9904..9906),aa:Ser,seq:gga)
     gene            complement(10194..10412)
                     /gene="infA"
                     /locus_tag="OO230_001842"
     CDS             complement(10194..10412)
                     /gene="infA"
                     /locus_tag="OO230_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5691631.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10697..11401)
                     /gene="aat"
                     /locus_tag="OO230_001843"
     CDS             complement(10697..11401)
                     /gene="aat"
                     /locus_tag="OO230_001843"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5691632.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11443..13164)
                     /gene="cydC"
                     /locus_tag="OO230_001844"
     CDS             complement(11443..13164)
                     /gene="cydC"
                     /locus_tag="OO230_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5691633.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13165..14931)
                     /gene="cydD"
                     /locus_tag="OO230_001845"
     CDS             complement(13165..14931)
                     /gene="cydD"
                     /locus_tag="OO230_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5691634.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15054..16019)
                     /gene="trxB"
                     /locus_tag="OO230_001846"
     CDS             complement(15054..16019)
                     /gene="trxB"
                     /locus_tag="OO230_001846"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5691635.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16563..17057
                     /gene="lrp"
                     /locus_tag="OO230_001847"
     CDS             16563..17057
                     /gene="lrp"
                     /locus_tag="OO230_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5691636.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17192..21220
                     /gene="ftsK"
                     /locus_tag="OO230_001848"
     CDS             17192..21220
                     /gene="ftsK"
                     /locus_tag="OO230_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5691637.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21375..21986
                     /gene="lolA"
                     /locus_tag="OO230_001849"
     CDS             21375..21986
                     /gene="lolA"
                     /locus_tag="OO230_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5691638.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21997..23340
                     /gene="rarA"
                     /locus_tag="OO230_001850"
                     /gene_synonym="mgsA"
     CDS             21997..23340
                     /gene="rarA"
                     /locus_tag="OO230_001850"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5691639.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23431..24723
                     /gene="serS"
                     /locus_tag="OO230_001851"
     CDS             23431..24723
                     /gene="serS"
                     /locus_tag="OO230_001851"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5691640.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24963..27406
                     /gene="dmsA"
                     /locus_tag="OO230_001852"
                     /pseudo
     CDS             24963..27406
                     /gene="dmsA"
                     /locus_tag="OO230_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27417..28034
                     /gene="dmsB"
                     /locus_tag="OO230_001853"
     CDS             27417..28034
                     /gene="dmsB"
                     /locus_tag="OO230_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5691641.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28036..28827
                     /locus_tag="OO230_001854"
     CDS             28036..28827
                     /locus_tag="OO230_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5691642.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28863..29489)
                     /locus_tag="OO230_001855"
     CDS             complement(28863..29489)
                     /locus_tag="OO230_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5691643.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29804..30952
                     /locus_tag="OO230_001856"
     CDS             29804..30952
                     /locus_tag="OO230_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5691644.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7686 a         7642 c         8367 g         7474 t
ORIGIN      
        1 ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt ttaaaaacga
       61 aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca acagcgggtt
      121 gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt taatccttta
      181 ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt tgcgggctta
      241 tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg ccatgcttaa
      301 atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg taaaggataa
      361 gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg gttttcgcgg
      421 tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg agaacgcgtg
      481 gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat cagatctcta
      541 ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc gagaacatca
      601 tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg ttcgccgtta
      661 tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta ttttttatcg
      721 tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa
      781 agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc tggtgcgttt
      841 aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta acggcacggt
      901 gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt
      961 gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt ccgcaatggt
     1021 gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat atcgtttaat
     1081 gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca tcaaccggat
     1141 gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt ttgctacttt
     1201 attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat tgctaattga
     1261 agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc tgttgaatct
     1321 tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt taactccagt
     1381 ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc gtctggggcc
     1441 aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt ttaatcgtcc
     1501 gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa cctgggttat
     1561 caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca aggtcattga
     1621 gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg ggattgaatg
     1681 gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg tacgcagcct
     1741 gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg cgctggatat
     1801 ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg cgcaaatccc
     1861 ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc gctcttccaa
     1921 acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg actccgtacc
     1981 aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc gcgcggatta
     2041 accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat agcgccgacg
     2101 gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta tctgtgctgg
     2161 aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga acgcgttgta
     2221 atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc
     2281 attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac aggccgcttc
     2341 catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct ggatttcctg
     2401 atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc ttttcccctg
     2461 atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt tgcggaacag
     2521 gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa gcgtgaactg
     2581 ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca aatataagct
     2641 gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta acgcctccag
     2701 caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca caggcagtcg
     2761 cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt tcatccactc
     2821 aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa aactacagcg
     2881 atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct
     2941 gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat tcatgaaatc
     3001 aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat caatagtgtg
     3061 attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg acccggattg
     3121 gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt
     3181 attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct taacgcaccc
     3241 gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg
     3301 accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag cggtcagttg
     3361 aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt aggggtcatt
     3421 gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat ggagctacgc
     3481 gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta ttcccgtcag
     3541 caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc cgcgacggag
     3601 atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg caatcaggcc
     3661 tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc gatggctggg
     3721 gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca gcaagcaccg
     3781 aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt ttctgaagcg
     3841 gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg cgatccactg
     3901 acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt aaacgacgct
     3961 attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg gctggatatg
     4021 accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat ccctctgtcg
     4081 gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg taatggtgaa
     4141 acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga gattgtcaaa
     4201 gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc tgcacaatga
     4261 cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat gagcaggttg
     4321 aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg attgttggcg
     4381 cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat aaggccacca
     4441 gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat gcgctggcgc
     4501 aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt ccgcatttaa
     4561 ccgtcgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg aaacagcgac
     4621 tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca gagtattatc
     4681 cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca ttgatgaacg
     4741 gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat tctggcgaag
     4801 aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt atcgtcaccc
     4861 acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac ggcgaaattg
     4921 tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag cctgttgtca
     4981 atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg acgatggcat
     5041 ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg attattatcg
     5101 gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa atggtgctgg
     5161 cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa gattttggcg
     5221 atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc atccaaaaac
     5281 aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg cgttataaca
     5341 atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat gtctatggca
     5401 tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt gcgcaggtcg
     5461 tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat gtggttggcg
     5521 aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt
     5581 cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg atgtccgggc
     5641 gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa ggatttgaca
     5701 gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt aagaaggatt
     5761 tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca cgtactttac
     5821 aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt ggtgtaatga
     5881 atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc atggctgtag
     5941 gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg gtttgcctgg
     6001 tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg cagcttttct
     6061 tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt ctctgctcga
     6121 cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga ctggatccag
     6181 tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc gggctggcat
     6241 tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg
     6301 acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg
     6361 gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa
     6421 atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac
     6481 ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga
     6541 ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta
     6601 gagaattagg gcgagccgtc aagcatttga caggggccag ggggcaggta tgaatcaaaa
     6661 atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag
     6721 atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca
     6781 gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac
     6841 tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct
     6901 atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg
     6961 ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac
     7021 taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg
     7081 atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc
     7141 tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt
     7201 ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac
     7261 ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg
     7321 gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg
     7381 cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa
     7441 cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg
     7501 gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc
     7561 agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag
     7621 tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg
     7681 gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga cccactaatt
     7741 ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac
     7801 ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg tcttgcctgg
     7861 cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta ctctctcgat
     7921 atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg
     7981 ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc
     8041 attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct aatcaaaccg
     8101 ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac
     8161 attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg
     8221 tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac
     8281 gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt
     8341 aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcagg cgctcgcgca
     8401 cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg
     8461 gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg
     8521 aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc cattgaggcg
     8581 ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt
     8641 tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg
     8701 aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc
     8761 gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg
     8821 accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg
     8881 catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac
     8941 aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa cgccggggta
     9001 cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg
     9061 gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg
     9121 ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat cgtcgagttg
     9181 caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg
     9241 ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag
     9301 gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt
     9361 caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca
     9421 caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtgaagtccg
     9481 taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa aaccctattt
     9541 ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga taaaaaaggg
     9601 tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg
     9661 agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc tttgcgaacg
     9721 cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac ccgcccacca
     9781 tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt cgtacgagct
     9841 cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc cgtatacaca
     9901 ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt gctaccaaac
     9961 ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc aagaagaatt
    10021 ttaatgataa ttattgtttg ctcatactgt aaacaacttg tgcagtatat ctacatcgag
    10081 acaagttatg gacttatact tccaaagtac ttcatacata tcacaaaata aaaaggccgg
    10141 ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa caatcagcga
    10201 ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac agtcactttg
    10261 tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg tgcagtaacc
    10321 acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc aagaacggta
    10381 ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc actaccgtaa
    10441 tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc gcgctaaatc
    10501 gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca ccaaaagcgc
    10561 aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa aattccttat
    10621 cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac tctggggaga
    10681 aatgtgccga aaacattcat tcttgtggtg aaaacaagca ccgtggtacc cagaaattat
    10741 tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg cggggaattt
    10801 cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata agcttaccgc
    10861 catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca ttttccatcc
    10921 ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac ataccgccga
    10981 caagctcatc ttcacgccag acttcaatgg agtgggcatg accgagttcg tgaaggcgat
    11041 ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg ctggcacagc
    11101 cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag cgtttatgaa
    11161 atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg cggggatcgg
    11221 gcgaccacca gaggatgggg tcgtctggag aaaaccacgg aaaaatacca cgctggtaag
    11281 ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg ttaggctcac
    11341 gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga accaagcgca
    11401 tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac cctgcttgaa
    11461 ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct caataatttg
    11521 cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc gatgggtgac
    11581 cattaacacc gttttctcac gcatcatttt tgcaagcaat tcaaggatct ggctttcggt
    11641 tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat catgtaacag
    11701 cgtacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc cgccttcacc
    11761 taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc gacgcaagat
    11821 ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat cacgcagtgt
    11881 ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta gagccgcttc
    11941 attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg ggtcccatgc
    12001 gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga gaatcgctat
    12061 atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg attgctccgg
    12121 ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac gagtttgggt
    12181 atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta cggcagaggc
    12241 aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt caaacgcggc
    12301 taacgcacaa aagacaaaca gggcaattaa tgcgccgggt tgagcattgc cgccaacgcc
    12361 gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca gcattatcgc
    12421 ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt gaatttctgt
    12481 attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct cagcttgccc
    12541 ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca gattttgccc
    12601 ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca gtaacataac
    12661 gctgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg tcaccaccat
    12721 aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat gatcgagcgt
    12781 atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg ccagtccggc
    12841 aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca acacgcggaa
    12901 agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg tgattgcggc
    12961 accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg caacccccgc
    13021 aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac tggcgagcag
    13081 cgtcacaatt gccagcacaa taccaagact taacatccat ttatgacgtt tatacagtgc
    13141 cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca gtaatgtggc
    13201 gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa tccggccatc
    13261 ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg tgaccattaa
    13321 cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt cactgtgagc
    13381 atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta gtaacgcacg
    13441 ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt cgccaacagg
    13501 tgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt tatccagcgc
    13561 tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat cccgcaattt
    13621 tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc atgattctgg
    13681 tgataaatca cgtaattcta tcccgttgat tcgtaatgat ccctgatatg agagaaaacc
    13741 agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cggccaacca acactgcacg
    13801 ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt ccggcgacgt
    13861 gataaacaga tcctcggcct caatggtcag cggatcggtc aatgctaatt ccgcctcacc
    13921 gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag ctgcaccaac
    13981 agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga aaaactctgg
    14041 cgcaaggatc agggccagaa aacctgcagc cagcgtcacc ccagtatcgt agtgaccaaa
    14101 atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa ttgacagcga
    14161 ggtaaaaaat tcgagaatgc cggaggataa aaacgccagt cgtagcactt ccattgtccg
    14221 ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac cacgaccaaa
    14281 aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta agcgagcaag
    14341 agcgagaaag ttacgccggt tagcatcggc agcccccatt ccaaccagcg ccataaacaa
    14401 cggaattagc ggtgctgtgc ccagcagaat gagcgccgca gcccagttag aggggaagat
    14461 cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca gatagcgtgc
    14521 atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc tccccgcagg
    14581 tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct gacggcggat
    14641 ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc agaccaccca
    14701 tgcgcgcaat acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg cttcacgggg
    14761 aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct gggcaatgat
    14821 caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt gggagatgac
    14881 gctttgctgt tttaaccagc gggttaactc tttttgacga gatttattca ttgcacgctt
    14941 agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa aaataaaggc
    15001 gacccatagt cgcaaggtgt cgccttcttt acttttgtta ctgatttgta aaattatttt
    15061 gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca gccagtaccg
    15121 gccgaagtaa tggcctggcg ataaatgtga tccatcacgt cgcctgcggc aaagacgcca
    15181 ggaatgctgg tctgggtggc attaccatga atacccgact gtactttgat gtagccgttt
    15241 tccagttcca gctgcccttc gaaaatcgca gtattcgggc tgtgaccgat agcaacaaac
    15301 agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg cagacgaacg
    15361 ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt gtgcagaatg
    15421 atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc cgcgcggaaa
    15481 ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata cagcgcctct
    15541 tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata gaagaaaccg
    15601 tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga gggcaggccg
    15661 agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta ttcgccgtta
    15721 tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg atcaaaaatg
    15781 atctcagttt caaacttggt ggcatgttcg tgcatgcgcg ccattaataa cggaccggtc
    15841 agatcgtttg gatcaccagg ccagttttcc acttccgtgg tggtggtcag ttggccgcct
    15901 ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta gacagcagcg
    15961 gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt cgtgcccatg
    16021 agatccccat agttgttggc agacaatggg caggattgta gggaatttac agacgtaaaa
    16081 aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata agcaggtcaa
    16141 attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc cgattaaatg
    16201 atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc gatgttttgc
    16261 tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaca gtattcttat
    16321 atgcgcataa ccatgtatgt aaataccatg tttaccgtgc tagtgaaatc tacgtatggc
    16381 gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct tctcaccgta
    16441 gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac gggtagcgac
    16501 tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga gagacaataa
    16561 taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat cgtaacattc
    16621 ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa cgtgtgggac
    16681 tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg tttattcagg
    16741 gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta ttcgttgaga
    16801 ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct gtacaaaaac
    16861 ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg ttgaaaacac
    16921 gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg cgtctgcctg
    16981 gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt aatcgtctgg
    17041 ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat tacactcctg
    17101 ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt tagcatcggg
    17161 caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg aagacaaaga
    17221 agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcattgc tgatccttat
    17281 tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt cggaccccag
    17341 ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc ccggtgcgtg
    17401 gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc ccgtcattat
    17461 tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca ttgattattt
    17521 tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct gtggtctggc
    17581 ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg gcagcttact
    17641 aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc tgctctgcgt
    17701 ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg ctgaaaaact
    17761 cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc gcgatgatac
    17821 ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa atcacggcaa
    17881 acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc gtaaacggtt
    17941 ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt tctccggtaa
    18001 gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg ccgacccgga
    18061 cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat acgatccatt
    18121 attaaacagt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta ccacggcgac
    18181 acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg cctctgttga
    18241 tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac aaacgggaga
    18301 gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg cgcagcctgc
    18361 agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt attatgcacc
    18421 tgcagctgaa caacctgcgc aacagccgta ttatgcccct gcgccagaac aaccggtggc
    18481 aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt ctacatacca
    18541 gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac agccgcaacc
    18601 cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa aacccgcgcg
    18661 tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac gtgaacaact
    18721 tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga tcaaatcttc
    18781 gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg ctgtttctcc
    18841 gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa ccgttgccgc
    18901 gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag aggggattgg
    18961 cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat tggcgtctta
    19021 cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag cccagcgcaa
    19081 tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc agcaggatga
    19141 actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt atcaacatga
    19201 tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg ctcgtcagtt
    19261 tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga atccgttctc
    19321 gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc cacacgaacc
    19381 gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg caccacagca
    19441 gcagtatcag cagccacaac agccggttgc gccacagcag cagtatcagc agccacaaca
    19501 gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc cacagcagca
    19561 atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc cgcaacaacc
    19621 ggttgcaccg cagccgcagt atcagcagcc acaacagcca gttgcgccac agccgcagga
    19681 taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc acaaaccgac
    19741 gacgccactg ccttctctgg atttgctgac accgccgccg agcgaagtgg agccggtaga
    19801 tacctttgcg cttgaacaaa tggcgcgtct ggtggaagcg cgtctggctg atttccgtat
    19861 taaagccgat gtcgttaatt actctccggg gccagttatc actcgctttg aacttaacct
    19921 ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg cccgttcact
    19981 ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg taggtctgga
    20041 gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata acgccaaatt
    20101 ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg gtgagccggt
    20161 ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg gttccggtaa
    20221 atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc cagaagatgt
    20281 gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag gcattccgca
    20341 cctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc gctggtgtgt
    20401 taacgagatg gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta atctggcggg
    20461 ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag acccgtactg
    20521 gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac catacattgt
    20581 cgtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag tggaagagct
    20641 gatagctcgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac tggcaactca
    20701 gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga cccgtatcgc
    20761 ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg gcgcggaatc
    20821 actgctgggt atgggggata tgctctactc tgggccgaac tccacgttgc cggtacgtgt
    20881 ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact ggaaagcgcg
    20941 tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag gtggtgcggg
    21001 tggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc agtttgtcac
    21061 tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg gttataaccg
    21121 tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac aggggcacaa
    21181 cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt gccggataag
    21241 gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt cttctttcct
    21301 caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg agtgacgtaa
    21361 tttgaggaat aatgatgaaa aaaattgcta tcacctgtgc attactctca agcttagtag
    21421 caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat aaagtcagca
    21481 gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg gtgcaggaag
    21541 gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg acacaacctg
    21601 atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg ttcgttgagc
    21661 aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg ctgattgccc
    21721 gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat gactttgtcc
    21781 tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg ggacgtgatg
    21841 gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt tatcagctga
    21901 aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg ccgcaaggcg
    21961 tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc gctcgatttt
    22021 tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt agcacagtat
    22081 atcggtcagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat cgaagccggg
    22141 catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac cctcgctgaa
    22201 gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac ctctggcgtg
    22261 aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg tcgccgcact
    22321 attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc atttctgcca
    22381 catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc gtcgtttgag
    22441 cttaattcgg cactgctttc ccgtgcccgt gtttatctgt tgaaatccct gagtacagag
    22501 gatattgagc aagtactaac tcaggcgatg gaagacaaaa cccgtggcta tggtggtcag
    22561 gatattgttc tgccagatga tacacgacgt gccattgcag aactggtgaa tggcgacgcg
    22621 cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcta tgatagcggt
    22681 aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag cgcccgcttt
    22741 gataacaaag gcgatcgctt ttacgatcta atttccgcac tgcataagtc ggtacgtggt
    22801 agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg tggcgatccg
    22861 ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa tgccgatcca
    22921 cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg cccggcggaa
    22981 ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa aagcaacgct
    23041 gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga ttatgacgtg
    23101 ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta cgggcaggaa
    23161 tatcgttatg ctcatgatga agcaaacgct tatgctgccg gtgaggttta cttcccgccg
    23221 gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg caagattggt
    23281 gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg ctaccgttaa
    23341 tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt ggccacatct
    23401 cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct gcgtaatgag
    23461 ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt agataagctg
    23521 ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct gcaagcggag
    23581 cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga tatcgagcct
    23641 ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc cgagctggat
    23701 gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc tgcagatgaa
    23761 gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg taccccgcgt
    23821 gagtttgact ttgaagttcg tgaccacgtg acgctgggtg aaatgcactc tggcctcgac
    23881 tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg gcagattgct
    23941 cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca gcatggctac
    24001 agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac gggtcagctg
    24061 ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc agacaccagt
    24121 aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg cggtgaaatc
    24181 atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt ccgttctgaa
    24241 gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt cgacaaagtt
    24301 gaaatggtgc agatcgtgcg cccagaagac tcaatggcgg cgctggaaga gatgactggt
    24361 catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat cctttgcact
    24421 ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat cccggcacag
    24481 aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc acgtcgtatg
    24541 caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac cctgaacggt
    24601 tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca gcaggctgat
    24661 ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga atatattggc
    24721 taatacccaa tttttctgaa tctaaaaagc gcctttaggg cgcttttttt gtctcccttt
    24781 gataccgaac aataattact cctcacttac acgtaatact actttcgagt gaaaatctac
    24841 ctatctcttt gattttcaaa ttattctatg tatacaagcc tatatagcga gctgctatag
    24901 aaataattac acaatacggt ttgttactgg aatcaatcgt gatcaagctt gagtgagcca
    24961 ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggagagtcgc cgtggtttgg
    25021 taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca ttacctttta
    25081 gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa aaggttatct
    25141 ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac gtcgtggacg
    25201 gtgaaatcaa atatgtcgaa acgacaatac cggcgatgac aattacgacg gcctacacta
    25261 ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc cggaccgcct
    25321 gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc gcattagctg
    25381 ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag agtacggcaa
    25441 cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga cccgctcctg
    25501 gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt atcttaacca
    25561 ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct acggcggctg
    25621 ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc tgtttggtaa
    25681 taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg aacaggcacg
    25741 ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca ccggtgccgg
    25801 gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg ttaacggtct
    25861 ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata aatattgcgt
    25921 tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt ataaagctta
    25981 tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct cgcaaatcac
    26041 cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta ccaaaccggc
    26101 gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg caacccgtgc
    26161 tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga acagcggcgc
    26221 gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa acccgatcca
    26281 gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg aaatgacggc
    26341 gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa
    26401 ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc atgaaatcct
    26461 ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga cctcatcggc
    26521 gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg actttgcact
    26581 ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga ttaaaccgcg
    26641 ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc ttggcgttga
    26701 gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt atgcccagtc
    26761 gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg ggatctttaa
    26821 aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag atccgcaggc
    26881 aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc tggctgacat
    26941 tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga tctacacgcc
    27001 gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc ttacaggttt
    27061 ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga aagcggcttg
    27121 ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc acaacggcga
    27181 taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag tgacgccacg
    27241 aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg atgcaaaacg
    27301 tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc ctctcgctaa
    27361 ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag taaccgatga
    27421 caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa acctgcgagc
    27481 tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc atttatgaat
    27541 atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg tttgcctact
    27601 atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc tgcccgagcg
    27661 gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta tgcattggct
    27721 gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa acgaaaggcc
    27781 atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag ccgatctgtg
    27841 ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg cgtaaaaaac
    27901 atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa ccgaatattg
    27961 tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat ctggcaaacc
    28021 cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat cttcacggtc
    28081 ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct caaaggcgac
    28141 ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg ggtactgatg
    28201 ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc ctttaactcg
    28261 ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc gatctttttt
    28321 gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc ggcattgcgt
    28381 acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat gatggtgcgt
    28441 gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc gatgggctgg
    28501 gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg cgtgatgtca
    28561 gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc cgcagcgctg
    28621 gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt tgccctgtgc
    28681 ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact atccgtctca
    28741 ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg tttgcatatg
    28801 accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc cccggttctc
    28861 ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata atcaggaata
    28921 tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg gtgcagctcg
    28981 caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg atcccacgcc
    29041 gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa gacatcaaac
    29101 ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac cacaccggca
    29161 ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc gttatcccag
    29221 gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc tttcagttct
    29281 agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt tggtaagttg
    29341 aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc gggttcgata
    29401 tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc agcatcattt
    29461 ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt
    29521 ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc
    29581 tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag atcaataaaa
    29641 acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt aatataaatc
    29701 gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt tttggcggtg
    29761 gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca cgtatacccg
    29821 gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg cggtgttaaa
    29881 tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg taggcgttgt
    29941 cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt atgtcattaa
    30001 acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg ctggctgtgc
    30061 cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg tcgcgggcgt
    30121 cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg ggacgtcacg
    30181 taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa cgtttttagg
    30241 ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt gggttacagg
    30301 tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc agcaggctga
    30361 aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc ggcttggcgt
    30421 gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga tgcctctgta
    30481 cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg tactggtcag
    30541 tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg gtcgactgct
    30601 ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc ttagccaggc
    30661 ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat atccggtggc
    30721 gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtggcgatga accaagcctt
    30781 actgttgagc tataccgtgg gaagtctgct tggcccgtca tttagcgcta tgctaatgca
    30841 gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta tctatttgct
    30901 gatgctgctt cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt aaatgaattc
    30961 aagcaaagtg tgaacttact gtttcacact ctgctttttt gtttcttcta tctgacttgc
    31021 ttttttccaa attttattcg tttaaaaata aaatgtgcag caggttataa ttttgcattt
    31081 cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga tgccccttta
    31141 attttggcga aggatttgtc tatggctgg
//
LOCUS       DAJTPG010000038        30545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTPG010000038 DAJTPG010000000
VERSION     DAJTPG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..155)
                     /locus_tag="OO230_001857"
     CDS             complement(<1..155)
                     /locus_tag="OO230_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2517 family protein"
                     /protein_id="HCR5691645.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTS"
     gene            468..557
                     /gene="kdpF"
                     /locus_tag="OO230_001858"
     CDS             468..557
                     /gene="kdpF"
                     /locus_tag="OO230_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5691646.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            557..2230
                     /gene="kdpA"
                     /locus_tag="OO230_001859"
     CDS             557..2230
                     /gene="kdpA"
                     /locus_tag="OO230_001859"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5691647.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2253..4300
                     /gene="kdpB"
                     /locus_tag="OO230_001860"
                     /pseudo
     CDS             2253..4300
                     /gene="kdpB"
                     /locus_tag="OO230_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4309..4881
                     /gene="kdpC"
                     /locus_tag="OO230_001861"
     CDS             4309..4881
                     /gene="kdpC"
                     /locus_tag="OO230_001861"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5691648.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4874..7554
                     /gene="kdpD"
                     /locus_tag="OO230_001862"
                     /pseudo
     CDS             4874..7554
                     /gene="kdpD"
                     /locus_tag="OO230_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7551..8236
                     /gene="kdpE"
                     /locus_tag="OO230_001863"
                     /pseudo
     CDS             7551..8236
                     /gene="kdpE"
                     /locus_tag="OO230_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8511..8615
                     /locus_tag="OO230_001864"
     CDS             8511..8615
                     /locus_tag="OO230_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5691649.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            8926..11123
                     /gene="speF"
                     /locus_tag="OO230_001865"
                     /pseudo
     CDS             8926..11123
                     /gene="speF"
                     /locus_tag="OO230_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11120..12439
                     /gene="potE"
                     /locus_tag="OO230_001866"
     CDS             11120..12439
                     /gene="potE"
                     /locus_tag="OO230_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5691650.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12495..14135)
                     /gene="pgm"
                     /locus_tag="OO230_001867"
     CDS             complement(12495..14135)
                     /gene="pgm"
                     /locus_tag="OO230_001867"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5691651.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14161..14706)
                     /gene="seqA"
                     /locus_tag="OO230_001868"
     CDS             complement(14161..14706)
                     /gene="seqA"
                     /locus_tag="OO230_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5691652.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            14891..15655
                     /gene="ybfF"
                     /locus_tag="OO230_001869"
     CDS             14891..15655
                     /gene="ybfF"
                     /locus_tag="OO230_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5691653.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15795..16088
                     /gene="ybfE"
                     /locus_tag="OO230_001870"
     CDS             15795..16088
                     /gene="ybfE"
                     /locus_tag="OO230_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5691654.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16228..16758
                     /gene="fldA"
                     /locus_tag="OO230_001871"
     CDS             16228..16758
                     /gene="fldA"
                     /locus_tag="OO230_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5691655.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            16968..17054
                     /gene="uof"
                     /locus_tag="OO230_001872"
     CDS             16968..17054
                     /gene="uof"
                     /locus_tag="OO230_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5691656.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17047..17493
                     /gene="fur"
                     /locus_tag="OO230_001873"
     CDS             17047..17493
                     /gene="fur"
                     /locus_tag="OO230_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5691657.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17577..17903)
                     /locus_tag="OO230_001874"
     CDS             complement(17577..17903)
                     /locus_tag="OO230_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691658.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(17940..19346)
                     /gene="chiP"
                     /locus_tag="OO230_001875"
     CDS             complement(17940..19346)
                     /gene="chiP"
                     /locus_tag="OO230_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5691659.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(19925..21589)
                     /gene="glnS"
                     /locus_tag="OO230_001876"
     CDS             complement(19925..21589)
                     /gene="glnS"
                     /locus_tag="OO230_001876"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5691660.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21792..23738)
                     /gene="nagE"
                     /locus_tag="OO230_001877"
     CDS             complement(21792..23738)
                     /gene="nagE"
                     /locus_tag="OO230_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5691661.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24071..24871
                     /gene="nagB"
                     /locus_tag="OO230_001878"
     CDS             24071..24871
                     /gene="nagB"
                     /locus_tag="OO230_001878"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5691662.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            24931..26079
                     /gene="nagA"
                     /locus_tag="OO230_001879"
     CDS             24931..26079
                     /gene="nagA"
                     /locus_tag="OO230_001879"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5691663.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26088..27308
                     /gene="nagC"
                     /locus_tag="OO230_001880"
                     /gene_synonym="nagR"
     CDS             26088..27308
                     /gene="nagC"
                     /locus_tag="OO230_001880"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5691664.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27356..28108
                     /gene="nagD"
                     /locus_tag="OO230_001881"
                     /pseudo
     CDS             27356..28108
                     /gene="nagD"
                     /locus_tag="OO230_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28505..30169
                     /gene="asnB"
                     /locus_tag="OO230_001882"
     CDS             28505..30169
                     /gene="asnB"
                     /locus_tag="OO230_001882"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5691665.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         7245 a         7747 c         7978 g         7575 t
ORIGIN      
        1 tcgctggttt tcgaccagac gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag
       61 aaaagcatga aaggcagcgc cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag
      121 ataagccatg caggatattc tctgtagagt tccatacttt tctccccctt ttgtatctac
      181 ccggtgaata gcaccggaaa aatgaatttg tttatctgat gaaaatagta ccgccttttg
      241 tgtaatttta ctactcatcc gaccacttat ttttgcttat tgatggttta tttacattca
      301 cactgtaatt aaattacaca aaagttaaat taatactaac catcagttaa atcatggcct
      361 ttgccatttt tatacttttt ttacaccccg tccgcagatt tttgcgaaat ctttgcagcc
      421 agaattctac tcttccggta tcacttttag gccactggag gtgcactgtg agtgcaggcg
      481 tgatagccgg cgtgttgctg gtgtttttat tactgggcta tttggtttat gccctgatca
      541 atgcggaggc gttctgatgg ctgcgcaagg gttcttactg attgccacgt ttttactggt
      601 attaatggtg ctggcgcgtc ctttaggcag cgggctggcg cggctgatta atgacattcc
      661 tcttccaggt acagcgggcg ttgaacgtat actctttcgt ttacctggcg tctctgacca
      721 tgagatgaac tggaagcaat atctttgtgc cattctcggc ctgaacatgc tggggctggc
      781 ggtgctgttt tttatgttgc tcggtcagca ctatctgccg cttaatccac agcagttgcc
      841 ggggctgtcg tgggatctgg cgctgaatac cgccgtcagc tttgtcacga ataccaactg
      901 gcaatcttat agcggtgaaa ccacgttgag ctatttcagc cagatggcag gcttaacggt
      961 acaaaacttt ctttctgccg ccagcgggat tgcggtgatt tttgccttca tccgtgcgtt
     1021 tactcgccag agcatgagca cactcgggaa tgcctgggtc gatctgctac gcatcacgtt
     1081 atgggtgcta gtccctgtgg cgttgttgat cgcactgttt tttattcaac aaggtgcgca
     1141 gcaaaacttt ctgccctatc aggcagtgaa taccgttgaa ggagcgcaac agctgttacc
     1201 catgggacca gtagcttctc aggaagcgat caagatgctc ggtactaacg gcggtggctt
     1261 ctttaatgcc aactcgtcgc atccgtttga aaacccaacc gcactgacca acttcgtgca
     1321 gatgctggcg atcttcttga tcccaacagc gctgtgcttt gcctttggtg aagtgacggg
     1381 cgatcgccgc caggggcgca tgttgctgtg ggcgatgtca gtgatttttg tcatctgcgt
     1441 aggcgtggtt atgtgggcag aagttcaggg taatcctcat ctgctggcgc tcggcgcaga
     1501 cagcagcatc aatatggaag gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt
     1561 tgcggtcgtg actacggcgg cgtcctgtgg cgcggtgatt gcgatgcatg attcgtttac
     1621 cgctctcggc ggcatggtgc cgatgtggct aatgcaaatt ggtgaagtgg tgttcggcgg
     1681 tgtcggttct ggtctttacg gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg
     1741 gctgatgatt ggtcgtacac cggaatatct gggtaaaaaa atagacgtac gcgagatgaa
     1801 actgactgca ctggcgattc tggttactcc aacactggta ctgatgggcg cggcgttggc
     1861 gatgatgacc gacgccggac gtagcgccat gcttaaccct agcccgcatg gttttagcga
     1921 agtgctgtac gccgtgtcgt ccgccgctaa caacaacggc agcgcctttg ccggattaag
     1981 cgccaactct ccgttctgga actgtttact ggcgttctgc atgtttgtcg gtcgcttcgg
     2041 ggtgattatc ccggtgatgg caattgccgg ttcgctggtg agtaaaaaga gccaacccgc
     2101 cagctccggc acgctgccaa cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt
     2161 gttgctggtt ggcgcactga cctttatccc tgccctggcg cttggtccgg tggcggaata
     2221 tctctcctga tgatattgag tgagcactga atatgagtcg taaacaactg gcgctattcg
     2281 aaccaacact tgtcgttcag gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc
     2341 aatggcgcaa tccggtgatg tttatcgtct ggatcggcag tctgctgacc acctgtatta
     2401 gcatcgcgat ggcaagcggt gcgatgcctg gcaatgcgct gtttagcgcg gcaattagcg
     2461 gctggctgtg ggtcaccgta ctgttcgcta atttcgccga ggcgctggca gaaggccgca
     2521 gtaaagcgca ggccaacagt cttaaagggg tgaaaaaaac tgcctttgcc cgcaagctgc
     2581 gtgagccgaa atatggcgct gcggcggaca aagttcctgc cgatcaactg cgtaaaggtg
     2641 acatcgtgct ggtagaagct ggcgatatta tcccctgcga tggtgaagtc attgaaggcg
     2701 gcgcatcggt cgatgaaagc gccatcaccg gggaatcggc accggtgatc cgtgaatccg
     2761 gcggcgattt tgcctccgtc accggcggca cgcgtattct ttctgactgg ctggtgattg
     2821 agtgtagcgt taaccccggt gagacatttc tggatcggat gatcgcgatg gtggaaggcg
     2881 cacagcgacg caaaacaccg aacgagattg ccctgactat cctgctgatt gctctgacca
     2941 tcgtcttttt actggcaacc gccacgctgt ggccgttttc cgcgtggggc ggtaatgccg
     3001 tgagtgtaac ggtactggtg gcgctgctgg tctgtctgat cccaaccact attggtggcc
     3061 tgttgtcagc gatcggcgtc gccgggatga gtcggatgct gggcgcgaat gtgattgcca
     3121 ccagcggacg tgcagttgaa gcggcaggtg acgttgacgt tctgctactg gataaaaccg
     3181 gcaccatcac actcggtaac cgtcaggcgt cggagtttat ccccgcgcag ggcgtggatg
     3241 aaaaaacgct ggctgacgcc gcacaactgg cttcgctggc tgatgaaacg ccggaaggtc
     3301 gcagtattgt gatcctcgcc aagcaacgtt ttaacctgcg cgagcgcgat gtgcagtcgc
     3361 tccatgccac ctttgtaccg tttactgcgc aaagccggat gagcgggatc aacatcgaca
     3421 accgcatgat ccgtaaaggt tctgtcgatg ccattcgccg ccatattgag gctaacggcg
     3481 gtcacttccc tgccgatgtt gatcaaaaag tcgatcaggt tgcgcgtcaa ggagccacgc
     3541 tgctggtggt ggtggaaggt tctcgtgtgc tgggcgttat tgcgctgaaa gatatcgtca
     3601 aaggcggtat taaagagcgc ttcgcccagc tgcgcaaatg ggcattaaaa cggtgatgat
     3661 taccggcgat aaccgtctga ctgccgccgc gatagctgcg gaagcgggtg tcgatgattt
     3721 tctcgccgaa gcgacaccgg aggccaagct ggcattgatc cgtcagtatc aggcggaagg
     3781 tcgtttggta gcgatgaccg gcgacagcac caacgatgct acggcgctgg cgcaggcaga
     3841 tgtcgcggtg gcgatgaact ccggcaccca ggcggctaaa gaggcgggca atatggtgga
     3901 tctcgactct aacccgacca agttgatcga ggtggtgcac attggcaaac agatgctgat
     3961 gacccgtggc tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat
     4021 tattccggcg gcgttcgcgg caacatatcc gcagttaaat gcgctgaaca tcatgcgcct
     4081 gcattcgccc gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt
     4141 tttgattcct ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt
     4201 gcgccgtaac ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa
     4261 agtcattgat ttactgctaa ccgtttgtag tctggtgtga ggtttaccat gagtggatta
     4321 cgtccggcat tatcgacatt tctctttctg ttattgatta ctggcggcgt ttacccgctg
     4381 ctgaccaccg cactggggca atggtggtat ccctggcagg ccaacggttc gttgattcgt
     4441 gaaggtgata cggtgcgcgg ttcggcatta atcgggcaaa attttaccgg caacggctat
     4501 tttcatggtc gcccgtcggc aacagcagaa atgccctata atccacaggc ttctggcggg
     4561 agcaatctgg cagtcagtaa cccggagctg gataaacaga tagccgcacg cgttgccgca
     4621 ttacgggccg ctaacccgga cgccagcacg aacgttccgg ttgaactggt gacggcgtcg
     4681 gcaagcggac tggacaataa catcaccccg caagcggcgg cctggcagat cccacgcatc
     4741 gcaaaagcgc gtaatctcag cgttgaacag ctcacgcagc tgatcgcaaa atacagccaa
     4801 caaccgctgg tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggca
     4861 ctggataagc ttgatgaata acgaaccctt acgtcccgac cccgaccgtc tgctggaaca
     4921 aactgccgcg ccgcatcggg ggaagctgaa agttttcttc agtgcctgtg caggcgtcgg
     4981 gaagacctgg gcgatgctgg cagaagccca gcgactgcgg gcgcaagggc tggatattgt
     5041 ggttggtgtg gtagaaaccc atgggcggaa agataccgcc gccatgcttg aagggctggc
     5101 tgttctgcct ctgaaacgcc aggcgtaccg tgggcggcat atcagcgagt ttgatctcga
     5161 tgccgccctc gcccgccgtc cggcgctgat cttaatggac gaactggcgc acagtaatgc
     5221 gccaggttct cgtcatccta aacgctggca ggatatcgaa gaactgctgg aagctggcat
     5281 tgatgttttc acaaccgtca acgttcagca tctggaaagt ctgaatgatg tggtcagcgg
     5341 cgtcaccgga attcaggtac gggaaaccgt gcccgatcct tttttcgatg ccgccgacga
     5401 cgtggtgctg gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt
     5461 ctatattgcc gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gcaatctgat
     5521 cgccctgcgc gaactggcac tgcgccgtac tgccgatcgc gttgatgagc aaatgcgcgc
     5581 ctggcggggg catcctggcg aagagaaagt ctggcacacg cgcgacgcga tccttttatg
     5641 catcggccat aacaccggca gcgaaaactg gtccgcgcgg cggcgcggct gacgtcacgg
     5701 ctggggagcg tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg cttaccggag
     5761 aaaaaacgtc gggcaattct cagcgcctta cgtctggcgc aggagctggg cgcggagacg
     5821 gcaacacttt ctgatccagc ggaagagaaa gcggtagtgc gttatgcccg tgaacataat
     5881 ctcggcaaga ttattctcgg tcgcccagcc tcgcgccgct ggtggcgtcg ggaaacattt
     5941 gctgaccgac tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc gcttgatgaa
     6001 ccacccgccc gcacgattaa caacgcgccg gatagccgct catttaaaga caagtggcgg
     6061 gtacagattc agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac cttaattgcc
     6121 atgcagtggc tgatggcgtt tgatgccgcc aacctggtga tgctgtatct gcttggcgtg
     6181 gtggtggcgc tattttatgg acgctggcct tcggtggttg ccaccgtcat taatgtggtg
     6241 agtttcgatc tcttttttat cgccccacgc ggcacgctcg ccgtctctga tgtgcaatat
     6301 ctgctgacct tcgcggtgat gttaaccgtc gggctggtga tcgggaacct tactgctggt
     6361 gtgcgttatc aggcgcgggt agcccgttac cgcgagcaac gcacacggca cttatatgaa
     6421 atgtcgaaag ctctggcggt gggccgcagt ccgcaggaca tcgccgccac cagcgaacaa
     6481 tttattgcct ccacgtttca tgcccgcagt caggtgttgt tgcccgatga caacggtaaa
     6541 ttgcagccgt taacacatcc gcaaggaatt acgccatggg acgatgccat cgcgcagtgg
     6601 agttttgata aaggcctgcc tgcgggcgcg ggcaccgaca cgttacccgg tgtaccgtac
     6661 cagattttgc cgctaaaaag cggcgagaaa acctacgggc tggtggtggt ggagccgggg
     6721 aatctgcgcc agttgatgat cccggaacag cagcgcctgc tggagacttt tacgctgtta
     6781 gtcgccaatg cccttgagcg gctgacgcta accgccagcg aagaacaggc gcggatggca
     6841 agcgaacgtg aacagatccg caacgccctg ctggcagcgc tttcgcatga tttacgcacg
     6901 ccgcttacgg tgctgtttgg tcaggcagaa atcttaacgc tcgatctggc aagcgaagga
     6961 tcaccccacg cccgccaggc cagcgagatt cgtcagcatg tgctgaacac tacccgactg
     7021 gtgaataatc tactggatat ggcgcgaatt cagtccggcg gctttaattt gaagaaagag
     7081 tggttaacgc tggaagaagt agtcggcagc gcgctgcaaa tgccggaacc gggtttattg
     7141 tcgcccatca atctttctct gccagaaccg ctgaccttaa tccacgttga cgggccactc
     7201 tttgaacggg tgctgattaa tctgctggag aacgcggtga aatatgcggg tgcgcaggcc
     7261 gaaattggta tcgatgccca cgttgagggc gaaaatctac aactggatgt ctgggataac
     7321 ggccccggtc ttccgccagg ccaggagcag acgatatttg ataagtttgc tcgcgggaat
     7381 aaagagtcgg cggtgccggg tgtcgggctt gggttggcaa tttgtcgggc gattgtggat
     7441 gtacacggcg gcaccattac cgcgttcaac cgaccggaag gtggtgcctg ttttcgtgtt
     7501 acacttcccc agcaaaccgc tcctgaactt gaagactttc atgaggatat gtgacaaacg
     7561 ttctgattgt tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg
     7621 acgggatgcg cgtctttgag gccgaaactc tgcaacgcgg cttgctggaa gcggcaaccc
     7681 gtaagccaga tttgattatt ctcgatctcg gcctgcccga tggtgatggg attgagttta
     7741 tccgcgacct gcgccagtgg agcccggtgc cggtgattgt gctttccgca cgcagcgaag
     7801 agagcgacaa gatcgccgcg ctggatgccg gagcggatga ttatctgagt aagccgtttg
     7861 gtattggcga attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc gccactccgc
     7921 caccgccgcg cccgatccgc tggtgaaatt ttccggtgtt accgtcgatt tagccgcccg
     7981 cgtgattcac cggggtgagg aagaggtgca tctcacacca attgagttcc gcctgctggc
     8041 ggtgctgctc aacaatgccg gaaaagtact cacccagcgc cagctcctta accaggtgtg
     8101 ggggccaaac gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca
     8161 aaaactggaa caggatccgg cccggcctcg ccacttcatt actgaaaccg gtattgggta
     8221 tcggtttatg ctttgaattg ttattcaata aattcgctta aatagtgatt aatttattat
     8281 taaacctgcg ccgaatatta ttatttggca ggtttaattt cttaacgcaa atataaaaca
     8341 cgaaattaca acatttaagt aacaaaacag catctaatat ggcgatattc ataattaatg
     8401 atttattatc tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc
     8461 atggatagaa taactctacc ttcaactgac acagcaagcg gtaaaggtaa atggaaaata
     8521 ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt gcccatcgta
     8581 atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac
     8641 gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata
     8701 tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat
     8761 gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac
     8821 tgatttattt atcagcggtg gagctttgct ttttttccga cgtgatcgat ttctcctttg
     8881 agaaattgag gacctgctat tacctgaaat aaagagatga ataaaatgtc agaattaaaa
     8941 attgccgtta gccgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatatt
     9001 gacgaaagta attatattga cgttgccgcc attattttat cagtcaatga tgttgaacgt
     9061 ggaaaactcg ataaaataga cgctactggc tatggcattc ctgtttttat tgcaacggaa
     9121 aatgaagaac gtgtaccagc agaatatctg ccacgtattt ctggtgtctt cgagcattgc
     9181 gaatcacgga aagagtttta tggacgtcag ttagaaaccg ctgctagcca ttatgaaacg
     9241 caactgcgcc cgcccttctt ccgcgcactg gtcgattatg tcaatcaggg caatagcgct
     9301 tttgactgcc cgggtcatca gggaggtgag tttttccgtc gtcatccggc agggaatcag
     9361 tccgtggagt acttcggaga aacgctgttc cgttccgact tatgcaacgc agacgtagca
     9421 atgggcgatc tgctgattca cgaaggcgcg ccatgcattg cacagcaaca tgcggcaaaa
     9481 gtgtttaatg ccgataaaac atacttcgtt ctaaatggca cttcatcttc taacaaagtg
     9541 gttttaaacg ccctgctaac accgggtgat ctggtgctgt ttgatcgcaa taaccacaaa
     9601 tctaaccacc acggagcgtt gctacaggct ggtgcaacac cggtttatct ggaaacggca
     9661 cgtaacccgt atggctttat cggtggcatt gatgcgcact gttttgaaga aagttacctg
     9721 cgtgagctga tcgcggaagt cgcaccgcag cgggcaaaag aggctcgtcc tttccgcctc
     9781 gctgtgattc agttaggcac ctacgacggt acgatttata acgcccgcca ggtggtggat
     9841 aaaattggtc atctgtgtga ctacattctg tttgactccg catgggttgg ctatgagcaa
     9901 tttatcccga tgatggctga ctgttcgccg ctgttgctgg atcttaatga gaacgatccg
     9961 ggtattctag ttacgcaatc cgtacataaa cagcaggctg gtttttctca aacttcacaa
    10021 atccataaaa aagacagcca catcaaaggg caacagcgtt atgtaccgca caaacgcatg
    10081 aacaacgcct ttatgatgca cgcctccacc agcccgttct atccgctgtt tgccgcactg
    10141 gatatcaacg ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg
    10201 gtaaatggca ttaatgcccg caaactgatc ctcgataact gtcagcatat tcgtccgttc
    10261 gtacctgaac tggtggatgg taaaccctgg cagtcgtatg aaacagcgca aattgcggtt
    10321 gatctgcgct tcttccagtt tgtaccaggg gaacactggc attcttttga aggctatgca
    10381 gagaatcaat actttgtcga tccttgcaaa ctgttgctga caaccccagg tattgatgca
    10441 cgtaacggcg aatatgaagc gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc
    10501 gaaaatggcg tagtgccgga aaaatgcgat cttaacgcca tcctcttcct gctgactccg
    10561 gcagaagata tggccaaact tcagcaactt gtgccctgct ggtacgcttc gaaaaactgc
    10621 ttgagtccga cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc
    10681 gctacgccgg ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc
    10741 acaacgtgaa acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca
    10801 tgaatccgca agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctac
    10861 cggatgccga aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt
    10921 gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag
    10981 aagggatcaa cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac
    11041 atgatggtcg taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc
    11101 tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt
    11161 aaccatactg acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct
    11221 tgccgaagtt gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc
    11281 accggcatgg gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg
    11341 ttatgccgag tatgctttcg gtaaatccgg taactttatt gcgaactata cctacggcgt
    11401 ctcactgctg attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact
    11461 gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat
    11521 ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt
    11581 gtgggggggc attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc
    11641 gacgctttac gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc
    11701 ttccatcgct atgacgctgt gggcttttct tggtctggag tctgcgtgtg cgaatactga
    11761 tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc
    11821 ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct
    11881 ggcaaattca acggcaccat ttggcctggc cttcgcacag atgtttacac cagaagtggg
    11941 taaagtcatt atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt
    12001 caccattgcc caggtgttta aatcttcatc tgatgaaggc tacttcccta aaattttctc
    12061 ccgtgtaacc aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag
    12121 cgggttggcg ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa
    12181 cctggccgtg gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat
    12241 tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt
    12301 tggcgcgatg tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg
    12361 ttccatcgtg accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact
    12421 gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgctctttt
    12481 tttattaaat gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc
    12541 tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct
    12601 tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgcca
    12661 atagaagcac cgttgcccgg agcagcagtc aggcgcgcgg tgatcgggtc acctgccagg
    12721 gtgctggcgc tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc
    12781 gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag
    12841 tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacataatg
    12901 atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg
    12961 ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa
    13021 cctaccggta cttctaccag cttacggccc aagtcattga ccacacggtc gatcatcgca
    13081 gaagaaacca gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac
    13141 aggtagttga tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg
    13201 ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt
    13261 gccagcagac ccgccatcgc acactcggag gagcagtcca tacggatcgc accgtcttta
    13321 tcgaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg
    13381 ttgtaatact cgccaatgcg cttccagtat tcgataccgg aaccgcccaa cggatcaacg
    13441 cccagcgtca ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggt cagcccttcc
    13501 acgaacggct gcaccagatc ctgctctttc acatagccgg atgccatcgc ttcgtcgagg
    13561 gagatacgct tcacgccttt caggccatcg gccatcagtg cgttggcctt gtcttccacc
    13621 actttggtga cgttagtatc agccgggcca ccatttggcg gattgtactt gataccacca
    13681 tcttccggcg ggttatggga cggtgtaatc acgataccgt ctgccagcgg gccacctttt
    13741 ttattgtgaa ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc
    13801 tgcacaatga catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc
    13861 tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca
    13921 cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc
    13981 gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct
    14041 tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt
    14101 tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt
    14161 ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat
    14221 gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt
    14281 gcctggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc
    14341 cgcaaagtac acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc
    14401 gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc
    14461 tgcgtattcg tccgaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt
    14521 gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc
    14581 agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc
    14641 gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt
    14701 tttcatctta atccagtgca gtgtcttagc tgaatacaag tcgccaagag tatatgaata
    14761 aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt
    14821 tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa
    14881 gaactttgcc atgaaattaa atatccgcgc gcaaactgca caaaaccagc acaataattc
    14941 tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg
    15001 cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc
    15061 gagagagccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata ccctggatgc
    15121 actgcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc
    15181 acttactgca ctggcccccg atcgcatcga taaactggtg gcgatcgata tcgcaccggt
    15241 cgactatcac gtacgccgtc atgatgagat tttcgcagct atcaacgcgg tcagtgaatc
    15301 ggacgcacaa actcgccagc aagcagcagc aataatgcgc cagcatctta atgaagaagg
    15361 ggtgattcag tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt
    15421 attgtgggat cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc
    15481 tgccctgttt atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt
    15541 actggcacaa tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca
    15601 tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa
    15661 ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg
    15721 cgctgatgta ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg
    15781 aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc
    15841 acgagcgtcg accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta
    15901 ttaataaacg caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga
    15961 agctgaacgc ggaagctgtc gaggcgctga acgagctggc ggaatcgcgt aatatgagcc
    16021 gtagcgaact gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca
    16081 tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg
    16141 caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc
    16201 aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac
    16261 accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc
    16321 gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcttacga cattctgctg
    16381 ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg actgggatga cttcttcccg
    16441 actctcgaag agattgattt caatggtaaa ctggttgcgc tgtttggttg tggtgaccag
    16501 gaagattacg ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg
    16561 cgcggtgcaa ccatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa
    16621 ggtctggcag atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa
    16681 ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac
    16741 gaaattctta atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat
    16801 aatgccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc tatacaatgt
    16861 cccagtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa
    16921 tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta
    16981 tctcaagagc aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga
    17041 ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct
    17101 cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat
    17161 ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta
    17221 ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc
    17281 gtatttgaac tgacacagca acatcaccac gatcacctga tttgcctcga ctgcggcaag
    17341 gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat
    17401 ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc
    17461 cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc tgaacgagaa aagccaacct
    17521 gcgggttggc ttttttatac aagggaaata agaaaagcga gagttacagc tctcacttat
    17581 ttgttattac tgcgtatttc ctgccagacc ttatcacaat cggctttcac cgcctgatca
    17641 tttccggttt gcgttgcacg cagacactgc tgataatcca gtacgcgtac actttcctgg
    17701 tcggcaaact gctgatgttg tttctctttc ttcagcacgt ttaacacgct ctggcaggct
    17761 tcaatttttt ctggcgaacc ttgcgcggtg ttgatacagg cgctgtatgc ctctttcaga
    17821 cggctgtctt cctgtggtgc ggttgattgc gcacaagcta ccagccccga tgccatcatg
    17881 gcgatgagaa tcagtttttt cattgaatgc ggcaggcaca aaacctgccg cgtcgggcat
    17941 cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc ctggaagatg
    18001 ttaccgtaac cgccgcccca gcttgggatg tcggagtggt tgtcgtattc ggtgaagtgc
    18061 agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc atccaggctg
    18121 taggcagact cttcaatagt ccggtttttg tcgtagtacg catccggatt gctctgccag
    18181 gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa gcctggaaga
    18241 ttccagtttt tcaggtcata catcgcactg aagaagaccg ctttttcgcc gttagcgttg
    18301 aagtcggaac ggttatccca ccagatatcc aggcggccgt tggaggaagc gtaggttggg
    18361 gtcatacgtt gcaggaagta tccctgctga ctgtcagcct taatccaggt gccttcgagg
    18421 cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag ccaggcggtg
    18481 ccgtcataaa gatcgttgac gctgcgatcg tcaactttat cgcgggtacc atagaactgg
    18541 tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt ggcaaaatat
    18601 tgatcgatat acccttccgc ctgaccaaat gccgcttcca gtacgaagtt atttttgaag
    18661 tcgtatttcg caccgatgga gtgcagataa tcaactttgg tggttttatc gttctgataa
    18721 aactcatcca tttccagatg ccacggcgct ttgtattcgt tggtccacat gtaggagaaa
    18781 ctcaaggcac cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta
    18841 cccggcataa agctccagtg cggcgctaac agcgtttgac cggttggctg aatgtaacct
    18901 gccctcgccc aaaccggacc gtatttaaat ttggccgcag ctttatacag gcttataccg
    18961 cttttgtcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg
    19021 ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc agcaatatca
    19081 aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcatt ccaggtggag
    19141 tgagaaaggt tggttttgta tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc
    19201 tgccagtaat agataccgcc ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc
    19261 tccggcattg ccataaagcc cgacattgct gtaacaccgg caatagccag cgccagcgta
    19321 ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa attgctgcac
    19381 caaaggtgaa cagcgaaaaa aaataaaaat taaaaaagta gcgttgagaa aagtctcgct
    19441 gactacagga acagactatt tttcgcgacg cgaattatca atctctttct gtaaccagaa
    19501 tgtaagaaca tgagcaaccg cacatatttt gatgattttt gttacaaggc ttaagtttgc
    19561 ggttgaaagg cttgcagaag aataaaaacg tgaaattcca gcaagatgaa agcggttaca
    19621 ttttatttaa ttatgttttg tgattttaaa tttattttat ggataaattg cgaagcggaa
    19681 ttaattcgcg aagaaaagca gaaaaaacgc cgcagagcgc ggcgtttggg attgtttgct
    19741 atttcttgcc ggatgcggcg tgaacgcctt atccggccta caaaagcacg caaattcaat
    19801 atattgcaga gatcatgtag gcctgataag cgaagcgcat caggcaattt agcgtttgaa
    19861 atttattgtc tatttattgc cggatgcgac gtaaatgcct cacccggctt ggtagaactt
    19921 aaaattactc gcctactttc gcccaggtat cacgcagccc aacggtgcgg ttaaataccg
    19981 gtttttcggc cgtggaatgg cggctgtcga ggcagaagta accttcacgc tcaaactgga
    20041 atgctttacc cgcaaccgca tctttcagcg acggttcagc aaagccctgt ttgatcacca
    20101 gcgattccgg gttaatcacc gacaggaaat catccgcagc acctgggtta ggcacgctga
    20161 acagacgatc atacaaacgg atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa
    20221 taacaccttt gactttacga ccatctgccg gatctttgct taaggtgtcg gcgtcataag
    20281 tacagaagat ggtggtgata ttaccttcgg catctttctc cacgcgttca gccttaatga
    20341 cgtaagcatt acgcagacgc acttctttac ccagcaccag acgtttgtac tgcttgttag
    20401 cttcttcgcg gaaatcggcg cgatcgatcc aaatttcacc gctaaacggc acctgacggc
    20461 tccccatttc cggtttgttc ggatggttcg gcatggtgac catttcgcct tcgccctgat
    20521 agttttcgat aaccagtttc accggatcga taaccgccat tgcgcgcggc gcattttcgt
    20581 tgagatcttc acggatgcag gattccagcg acgccatctc aatggtgttg tcctgcttgg
    20641 tcacgccgat gcgtttgcag aactcacgaa tagaagccgc agtgtaacca cgacgacgca
    20701 gaccggaaat ggtcggcata cgcgggtcat cccagccttc aacgtgcttg tcggtcacca
    20761 gcaggttcag cttacgcttg gacatcacag tgtattccag attcaggcgc gagaattcgt
    20821 actggcgcgg atgaacagga atggtgatgt tatccagtac ccagtcgtac agacgacggt
    20881 tgtcctggaa ctcaagcgta cacagagagt gcgtaatacc ttccagcgca tcgctgatgc
    20941 aatgggtgaa gtcgtacatc gggtagatgc accacttgtt gccagtctgg tggtgttcag
    21001 caaatttaat gcggtacagc accggatcgc gcattacgat aaatggcgac gccatgtcga
    21061 ttttcgcacg caggcaggct ttaccttctt caaaaccacc ggcacgcatt ttttcgaaca
    21121 gcgccaagtt ctcttcaacg ctgcggtcgc ggtacgggct gtttttaccc ggctgcgtca
    21181 gggtaccgcg gtattcgcgg atctgttccg gtgtcagttc atcaacgtac gccaggcctt
    21241 tattgatcag ttcgatcgca taggcgtgga gctgatcaaa ataatcggag gagtaacgga
    21301 cgttaccaga ccagtgaaaa cctaaccact ctacgtcgtt tttgatcgaa tcgacatact
    21361 cgatatcttc ttttaccggg ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt
    21421 cctgggcgat cccgaagttc aggcagatag atttcgcatg gccaatatgc agatagccat
    21481 tcggctccgg cgggaaacgg gtgtgtactg tgatgtgctt accactggcc agatcttcat
    21541 cgatgatctg acggataaag ttagtcgggc gggcttctgc ctcactcatc gtggattcct
    21601 caaagcgtaa acaacgtata acggcgtatg atcttataag cgggacaggc tgacaactgt
    21661 tagtttttta aaattgataa tctaacaagg attatgggga tgattgatga aaaaaaaagc
    21721 ggcagagaag atctccaccg cttgtcattg ttggatgcga cgctgccgtg tcgcatcagg
    21781 cataaagcag attacttttt gatttcatac agcggtgttt gacccgccac aacatggccc
    21841 tgagctttaa tgagcaagcc actgaaatcg tcgatattgc tgcaaaccac cgggctaatc
    21901 atcgagcggg cgttagcgtt caggtaatcc agatccattt ccagaatcgg ttgccctgcg
    21961 cttacctgcg caccctcttc caccagacgt ttaaagcctt taccttccag cgctacggtg
    22021 tcgataccca tatggacgac gatctccgcg cctttttcgg tttccaggca gaacgcgtgg
    22081 ttggtgttga agattttcac gattgtccct gcggctggtg atacgacgat tttatctgtc
    22141 ggtttcaccg ccacaccgtc acccaccgct ttgctggcga atgcttcgtc aggaacctga
    22201 tccagtgcca cgacatcacc ggtaatcggc gataccagct ccgcgataga taccgcgttt
    22261 ggtacagcct gcggttttgc tacaggcgcg gcagttgccg gagttgcttc agctgacgca
    22321 gcggctaccg gaccacgggc aacgactttc ttcatcgcat cgccgatgga ttctgctttc
    22381 gcgccaacaa tcacctgaat agtctgtttg ttcagtttca ctacaccaga agcacccaga
    22441 cgtttacaca tcgtatcgtt gacgcgggca gagtcagcca cggtcaggcg cagacgggta
    22501 atacaggcat caatcgcttt caggttgtca gtaccgccaa ccgcagcaat atagttggtt
    22561 gccagttgag tcagaccttc ttcagtattg ctgttggctt cttcagtaac gatctcgtct
    22621 tctttatctt cacgacccgg cgttttcagg ttgaacatgc ggataaccaa actgaacacc
    22681 acgaagtaga tagcgaagaa gataacgccc atcaccagca gcatccagac gttctggctg
    22741 gcggcaggca ggttatacat caacgcgtag tcgatagcac ccgcagagaa agagaagccg
    22801 gcatggatac ccagcagcgt tgccacaaac aggctgatac cggtcagcag tgcgtgcagg
    22861 aggtacagca gcggcgcaag gaacatgaac aggaattcca gcggctcagt cacaccggtc
    22921 aggaacgcag taacagcaac agaaagcagc atcccgccaa ccatcggacg acgctctttc
    22981 ggtgctgcga agtacatcgc cagcgccgca cccggcagac cgaacatcat gatcgggaag
    23041 aagccggaca tgaacatccc cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg
    23101 tggaaaaccg tacccgccgc gttggtgaat tcaccaatct ggaaccaggc gatggtgttc
    23161 agcacctgat gcagaccggt tgggatcagc agacggttga tgaaaccaaa gataccggaa
    23221 cccagcgcgc ccgcagaaac gatccactcg ccgcctgcat ggatagcgtg ctgtaccggc
    23281 ggccagacgt aaccaaaaat ggccgccagc accagacaga agaagccggt ggcgatcggc
    23341 acaaagcgtt tgccgccgaa gaagctcagg aagtccggca gtttaatatc ggaccaacgg
    23401 ttataggctg cgccaccaac cagaccggta atgatacccg ccagtacacc catgttaatt
    23461 tctgggttga tggtcaccat cgctttggtt aacacaaagt aacctaccgc acccgccagc
    23521 gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca caccgatggc gaagattaat
    23581 gcgaggttat caaaaattgc accgcccgcc tgggcaataa acgcaacgtt aagtaaatct
    23641 ggctgaccga atcgcagcaa cagcgccgcc accggcagca ccgcgatagg gagctgtaac
    23701 gccctaccga gtcgctggaa aaaacctaaa atattcatct tattccccct acgagaaccc
    23761 tatttggctc gtttaaagcc gtatttttat tttgctgcaa attttactgc cgatgttctg
    23821 taatcagatt gttagatcat ctgctacaga gtgtgtgaaa atttaattcg catcgcaaat
    23881 taaacgcgtg tcttttgtga gttttgtcac caaatatcgt tatcatcact cccttttact
    23941 ggctaaacca gaaaacttat tttatcattc aaaaaatcaa gtcggattga cgcctgtctg
    24001 cgcaaatcca ggttacgctt aaagatgtct aatccgccaa cggcttacat tttacttatt
    24061 gaggtgaata atgagactga tccccctgac taccgctgaa caggtcggca aatgggctgc
    24121 tcgccatatc gtcaatcgta tcaatgcgtt caaaccaact gccgatcgtc cgtttgtact
    24181 gggcctgccg actggcggca cgccgatgac cacctataaa gcgttagtcg aaatgcataa
    24241 agcaggccag gtcagcttta agcacgttgt caccttcaac atggacgaat atgtcggtct
    24301 gccgaaagag catccggaaa gctactacag ctttatgcac cgtaatttct tcgatcacgt
    24361 tgatattcca gcagaaaaca tcaaccttct caacggcaat gccccggata tcgatgccga
    24421 gtgccgccag tatgaagaaa aaatccgttc ttacggaaaa attcatctgt ttatgggcgg
    24481 tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac
    24541 tcgtatcaaa accctgactc atgacactcg cgtcgcgaac tctcgtttct ttgataacga
    24601 tgtcaatcag gtgccaaaat atgccctgac tgtcggtgtt ggtacactgc tggatgccga
    24661 agaagtgatg attatggtgc tgggtagcca gaaagcactg gcgctgcagg ccgccgttga
    24721 aggttgcgtg aaccatatgt ggaccatcag ctgtctgcaa ctgcatccga aagcgatcat
    24781 ggtgtgcgat gaaccttcca ccatggagct gaaagttaag actttaagat atttcaatga
    24841 attagaagca gaaaatatca aaggtctgta attgttatcc ctgccctggt tccttgctca
    24901 gggcaatatt ttttaaaatc ggggggcaga atgtatgcat taacccaggg ccggatcttt
    24961 accggccacg aatttcttga tgaccacgcg gttgttatcg ctgatggcct gattaaaagc
    25021 gtctgcccgg tagcggaact gccgccagag atcgaacaac gttcactgaa cggggccatt
    25081 ctctcccccg gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac
    25141 accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca
    25201 ggctgtacta actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc
    25261 gtgcgcgtta tgcgtgagta cctggcaaaa catccgaatc aggcgttagg tctgcatctg
    25321 gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata atccgaattt tgtgcgtaag
    25381 cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg acgtcattac caaagtgacc
    25441 ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg
    25501 gtttctgccg gtcactccaa cgcgacgttg aaagaagcaa aagccggttt ccgcgcgggg
    25561 attacctttg ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc
    25621 ctggcgggcg cgatcctcga cgaagctgac atttattgcg gtattatcgc tgatggcctg
    25681 catgttgatt acgccaacat tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg
    25741 gttaccgacg ccaccgcgcc agcaggtgcc aacattgaac agttcatttt tgcgggtaaa
    25801 acaatatact accgtaacgg actttgtgtg gatgagaacg gtacgttaag cggttcatcc
    25861 ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa
    25921 gtgctgcgta tggcgacgct ctatccggcg cgtgcgattg acgttgaaaa acgtctcggc
    25981 acgctcgccg caggtaaagt agccaacctg accgcattca cacctgattt taaaatcacc
    26041 aagaccatcg ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg
    26101 gacaagctca gataggtaat gttgatctcg taaaacagct taacagcgcg gcggtttatc
    26161 gcctgattga ccagtacggg ccaatctcgc ggattcagat tgccgagcaa agccagcttg
    26221 cccccgccag cgtaaccaaa attacgcgtc agcttatcga acgcgggctg atcaaagaag
    26281 ttgatcagca ggcctccacc gggggccgcc gcgctatctc catcgtcacc gaaacccgca
    26341 atttccacgc aatcggcgta cggcttggtc gtcatgacgc caccatcact ctgtttgatc
    26401 tcagcagcaa agtgctggca gaagaacatt acccgctgcc ggaacgtacc cagcagacgc
    26461 tggaacatgc cctgctgaat gccattgctc agtttattga tagctaccag cgcaagctgc
    26521 gcgagctaat cgccatttcg gtgatcctgc cagggcttgt tgacccggac agcggcaaaa
    26581 ttcattacat gccgcatatt caggtagaaa actgggggct ggtagaagca ctggaagaac
    26641 gttttaaagt gacctgtttc gttggtcacg atatccgtag tctggcgctg gcggagcact
    26701 acttcggtgc aagtcaggat tgcgaagact ccattctggt gcgtgtccat cgcggaaccg
    26761 gggccgggat tatctctaac gggcgcattt ttattggccg caacggtaac gtcggtgaaa
    26821 ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc
    26881 tggaaactat cgccgccaac gctgccattg aacaacgggt gttgaatctg ttaaagcagg
    26941 gctaccagag ccgtgtgcca ctggacgact gcaccatcaa aactatctgc aaagccgcga
    27001 acaaaggcga tagcctggcc tcggaagtga ttgagtatgt cggtcgtcat ctgggtaaaa
    27061 ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat tgttattgct ggtgaaatca
    27121 ccgaagccga taaagtgctg ctccctgcta ttgaaagctg cattaatacc caagcgctga
    27181 aggcgtttcg tactaatctg ccggtggtac gttctgagct ggatcaccgc tcggcaatcg
    27241 gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat tttgctccag catttgctgg
    27301 aaaattaatg tgcttttata gtggcgctta ttgttgtcaa tattctgggt agtccatgac
    27361 cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg cacgataacg tcgccgtacc
    27421 gggtgcagcg gaatttttgc acgggattat ggataaaggc ctgccgctgg tgttgctgac
    27481 caactatcct tcgcagactg ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga
    27541 tgtgccggat agcgtgtttt atacctctgc gatggcgacc gccgatttcc tgcgtcgcta
    27601 ggaaggcaag aaagcgtatg tggtgggcga aggcgcactg attcatgaac tgtacaaagc
    27661 cggtttcact attaccgatg tgaaccctga tttcgtgatt gttggcgaaa cacgttccta
    27721 caactgggac atgattcata aagcagccta tttcgtcgct aacggtgcac gctttatcgc
    27781 caccaacccg gacacccacg ggcgcggttt ttatcccgct tgtggcgcgt tgtgtgcagg
    27841 gattgagaaa atctccgggc gcaaaccgtt ctatgttggt aagcccagcc cgtggatcat
    27901 ccgcgcggca ttaaacaaaa tgcaggcgca ttcggaagaa acagtgattg tcggcgataa
    27961 cctgcgtacc gatattctgg cgggcttcca ggccggtctg gagacgattc tggtgctttc
    28021 tggtgtttca tcgctcgacg atatcgacag tatgccgttc cgcccaagct ggatttaccc
    28081 gtcagtcgct gaaatcgacg ttatctgaat accacccacg tctgcggacg tggttggctc
    28141 tgatgacaaa cgccaaactg cctgatgcgc tacgcttatc aggcctacgc agctcctgca
    28201 atatattgaa ttttcatgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
    28261 aacaaatcgc actttatcag caatttggcc cccgtaaaat ataaccattc aataaaaccc
    28321 gctaaaaatt atcgattcat caataaatac atcctcacac cagacccttt ttcaccaatc
    28381 gacaatcacc attacgtttt ttatttttgc actgccaaat agcttgcgca cagtgctgct
    28441 ttgcggcatt tttttaaaca agcaaacaca acaagcaaca aataccaggt taacggagaa
    28501 ggttatgtgt tcaatttttg gcgtattcga tatcaaaaca gacgcagttg agctgcgtaa
    28561 gaaagcactc gagctgtcac gcctgatgcg tcatcgtggc ccggactggt ccggtattta
    28621 tgccagcgat aacgccattc tcgctcacga acgtctgtca attgttgacg ttaacgcggg
    28681 ggcgcaacct ctctacaacc aacaaaaaaa ccacgtactg gcggtaaacg gtgaaatcta
    28741 caaccaccag gcattgcgcg ctgaatatgg cgatcgttat cagttccaga cagggtctga
    28801 ctgtgaagtg atcctcgcgc tgtatcagga aaaagggccg gaatttctcg acgacttgca
    28861 gggcatgttt gcctttgccc tgtacgacag cgaaaaagat gcctacctga ttggtcgcga
    28921 ccatctgggg atcatcccac tgtatatggg gtatgacgaa cacggtcagc tgtatgtggc
    28981 ctcagaaatg aaatcgctgg tgccagtttg ccgcacgatt aaagagttcc cggcggggag
    29041 ctatttgtgg agtcaggacg gcgaaatccg ttcttattat catcgcgact ggttcgactt
    29101 tgatgcagtg aaagataacg tgaccgacaa aaacgagctg cgtcaggcac tggaagattc
    29161 cgttaaaagc catctgatgt ctgatgtgcc ttacggtgtg ctactttctg gtggtctgga
    29221 ttcctcaatt atttccgcta tcaccaagaa atacgcagcc cgtcgcgtgg aagatcagga
    29281 acgctctgaa gcctggtggc cgcagttaca ctcctttgct gtaggtctgc cgggttcacc
    29341 ggatcttaaa gcagcccagg aagtggcaaa ccatctgggc acggtgcatc acgaaattca
    29401 cttcactgta caggaaggtc tggatgccat ccgcgacgtg atttaccaca tcgaaactta
    29461 tgatgtgacc actattcgcg cttcaacacc gatgtattta atgtcgcgta agatcaaggc
    29521 gatgggcatt aaaatggtgc tgtccggtga aggttctgat gaagtgtttg gcggttatct
    29581 ttacttccat aaagcgccca acgttaaaga actgcatgaa gagacggtgc gtaaactgct
    29641 ggccttgcat atgtatgact gcgcgcgcgc caacaaagcg atgtcagcct ggggcgtgga
    29701 agcacgcgtt ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgca ttaacccgca
    29761 ggataaaatg tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat gttttgagtc
    29821 atacctgccc gcaagcgtgg cctggcggca gaaagagcag ttctccgatg gcgtcggtta
    29881 cagttggatc gacaccctga aagaagtggc ggcgcagcag gtttctgatc agcaactgga
    29941 aactgcccgc ttccgcttcc cgtacaacac gccgacctca aaagaagcgt atctgtaccg
    30001 cgagatcttt gaagaactgt tcccacttcc gagtgccgct gagtgcgtgc cgggcggtcc
    30061 ttccgtcgcg tgttcttcag ctaaagcgat cgaatgggat gaagcgttca aaaaaatgga
    30121 cgatccgtct ggtcgtgcgg ttggcgttca ccagtcggcg tataagtaag aaatgcggta
    30181 ctgccccgga gaaatccggg gctgttttag ttattgcgct gcttgttgat tctgatatgc
    30241 agtaaggaaa tgttccgggt ttgccacatc actgtttgac aactcatcac tgtaggccag
    30301 tatttcatag attaatttcc caccactatt gttgaattcc gaatttcatc ctgtacgaca
    30361 aaagaatggc tgtagggtgg ggaaaaacac aaagtaaaca ataattgacg aatatagcgc
    30421 cacgctgttc gcaacctaac caaacagtca ctttcgagca attttccttg aaaaagaggt
    30481 tgacgctgca aggctctata cgcataatgc gccccgcaac gccgataagg tatcgcgaaa
    30541 aaaag
//
LOCUS       DAJTPG010000039        30295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTPG010000039 DAJTPG010000000
VERSION     DAJTPG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO230_001883"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO230_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5691666.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO230_001884"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO230_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5691667.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO230_001885"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO230_001885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5691668.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO230_001886"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO230_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO230_001887"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO230_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5691669.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO230_001888"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO230_001888"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5691670.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO230_001889"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO230_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5691671.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="OO230_001890"
     CDS             7489..7638
                     /locus_tag="OO230_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691672.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="OO230_001891"
     CDS             7839..9422
                     /locus_tag="OO230_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5691673.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO230_001892"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO230_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5691674.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO230_001893"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO230_001893"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5691675.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO230_001894"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO230_001894"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5691676.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="OO230_001895"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="OO230_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="OO230_001896"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="OO230_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="OO230_001897"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="OO230_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="OO230_001898"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="OO230_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5691677.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="OO230_001899"
     CDS             20752..22167
                     /locus_tag="OO230_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5691678.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="OO230_001900"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="OO230_001900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5691679.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="OO230_001901"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="OO230_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5691680.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="OO230_001902"
     CDS             24477..24809
                     /locus_tag="OO230_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5691681.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /gene="yegQ"
                     /locus_tag="OO230_001903"
     CDS             24956..26317
                     /gene="yegQ"
                     /locus_tag="OO230_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5691682.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /locus_tag="OO230_001904"
     CDS             complement(26659..26976)
                     /locus_tag="OO230_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691683.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="OO230_001905"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="OO230_001905"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5691684.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29136)
                     /locus_tag="OO230_001906"
     CDS             complement(28363..29136)
                     /locus_tag="OO230_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5691685.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO230_001907"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO230_001907"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5691686.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7011 a         8047 c         7829 g         7408 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 aaaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctattcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccggccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgat
//
LOCUS       DAJTPG010000040        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTPG010000040 DAJTPG010000000
VERSION     DAJTPG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO230_001908"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO230_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5691687.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO230_001909"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO230_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5691688.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO230_001910"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO230_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5691689.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO230_001911"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO230_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5691690.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO230_001912"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO230_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5691691.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO230_001913"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO230_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5691692.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO230_001914"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO230_001914"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5691693.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO230_001915"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO230_001915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5691694.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO230_001916"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO230_001916"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5691695.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO230_001917"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO230_001917"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5691696.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO230_001918"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO230_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5691697.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO230_001919"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO230_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5691698.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO230_001920"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO230_001920"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5691699.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO230_001921"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO230_001921"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5691700.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO230_001922"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO230_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5691701.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO230_001923"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO230_001923"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5691702.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO230_001924"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO230_001924"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5691703.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO230_001925"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO230_001925"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5691704.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO230_001926"
     CDS             21622..22212
                     /locus_tag="OO230_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5691705.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO230_001927"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO230_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5691706.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO230_001928"
     CDS             22684..23379
                     /locus_tag="OO230_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5691707.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO230_001929"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO230_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5691708.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO230_001930"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO230_001930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5691709.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO230_001931"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO230_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5691710.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO230_001932"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO230_001932"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5691711.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO230_001933"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO230_001933"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5691712.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7028 a         7824 c         8255 g         6905 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTPG010000041        29790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTPG010000041 DAJTPG010000000
VERSION     DAJTPG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            47..841
                     /locus_tag="OO230_001934"
     CDS             47..841
                     /locus_tag="OO230_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5691713.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            863..2272
                     /locus_tag="OO230_001935"
     CDS             863..2272
                     /locus_tag="OO230_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5691714.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2250..3029
                     /locus_tag="OO230_001936"
     CDS             2250..3029
                     /locus_tag="OO230_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5691715.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3026..3886
                     /locus_tag="OO230_001937"
     CDS             3026..3886
                     /locus_tag="OO230_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5691716.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4034..4609)
                     /locus_tag="OO230_001938"
                     /pseudo
     CDS             complement(4034..4609)
                     /locus_tag="OO230_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4626..4886)
                     /locus_tag="OO230_001939"
     CDS             complement(4626..4886)
                     /locus_tag="OO230_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5691717.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4877..6148)
                     /locus_tag="OO230_001940"
     CDS             complement(4877..6148)
                     /locus_tag="OO230_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5691718.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6226..6588)
                     /gene="queD"
                     /locus_tag="OO230_001941"
     CDS             complement(6226..6588)
                     /gene="queD"
                     /locus_tag="OO230_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5691719.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6907..8706
                     /gene="cysJ"
                     /locus_tag="OO230_001942"
     CDS             6907..8706
                     /gene="cysJ"
                     /locus_tag="OO230_001942"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5691720.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVYALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8706..10418
                     /gene="cysI"
                     /locus_tag="OO230_001943"
     CDS             8706..10418
                     /gene="cysI"
                     /locus_tag="OO230_001943"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5691721.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10492..11226
                     /gene="cysH"
                     /locus_tag="OO230_001944"
     CDS             10492..11226
                     /gene="cysH"
                     /locus_tag="OO230_001944"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5691722.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11490..11642
                     /locus_tag="OO230_001945"
     CDS             11490..11642
                     /locus_tag="OO230_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5691723.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11836..12927
                     /locus_tag="OO230_001946"
                     /pseudo
     CDS             11836..12927
                     /locus_tag="OO230_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13073..14110)
                     /gene="iap"
                     /locus_tag="OO230_001947"
     CDS             complement(13073..14110)
                     /gene="iap"
                     /locus_tag="OO230_001947"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5691724.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14362..15270
                     /gene="cysD"
                     /locus_tag="OO230_001948"
     CDS             14362..15270
                     /gene="cysD"
                     /locus_tag="OO230_001948"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5691725.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15272..16699
                     /gene="cysN"
                     /locus_tag="OO230_001949"
     CDS             15272..16699
                     /gene="cysN"
                     /locus_tag="OO230_001949"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5691726.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16699..17304
                     /gene="cysC"
                     /locus_tag="OO230_001950"
     CDS             16699..17304
                     /gene="cysC"
                     /locus_tag="OO230_001950"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5691727.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17354..17677
                     /locus_tag="OO230_001951"
     CDS             17354..17677
                     /locus_tag="OO230_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5691728.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17871..18182
                     /gene="ftsB"
                     /locus_tag="OO230_001952"
     CDS             17871..18182
                     /gene="ftsB"
                     /locus_tag="OO230_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5691729.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18201..18911
                     /gene="ispD"
                     /locus_tag="OO230_001953"
     CDS             18201..18911
                     /gene="ispD"
                     /locus_tag="OO230_001953"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5691730.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18911..19390
                     /gene="ispF"
                     /locus_tag="OO230_001954"
     CDS             18911..19390
                     /gene="ispF"
                     /locus_tag="OO230_001954"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5691731.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19387..20436
                     /gene="truD"
                     /locus_tag="OO230_001955"
     CDS             19387..20436
                     /gene="truD"
                     /locus_tag="OO230_001955"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5691732.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20417..21178
                     /gene="surE"
                     /locus_tag="OO230_001956"
     CDS             20417..21178
                     /gene="surE"
                     /locus_tag="OO230_001956"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5691733.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21172..21798
                     /gene="pcm"
                     /locus_tag="OO230_001957"
     CDS             21172..21798
                     /gene="pcm"
                     /locus_tag="OO230_001957"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5691734.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21938..23077
                     /gene="nlpD"
                     /locus_tag="OO230_001958"
     CDS             21938..23077
                     /gene="nlpD"
                     /locus_tag="OO230_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5691735.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23140..24132
                     /gene="rpoS"
                     /locus_tag="OO230_001959"
     CDS             23140..24132
                     /gene="rpoS"
                     /locus_tag="OO230_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5691736.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24252..24659)
                     /locus_tag="OO230_001960"
     CDS             complement(24252..24659)
                     /locus_tag="OO230_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5691737.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24806..25399
                     /locus_tag="OO230_001961"
     CDS             24806..25399
                     /locus_tag="OO230_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5691738.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25399..26346
                     /locus_tag="OO230_001962"
                     /pseudo
     CDS             25399..26346
                     /locus_tag="OO230_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26619..27182)
                     /locus_tag="OO230_001963"
     CDS             complement(26619..27182)
                     /locus_tag="OO230_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691739.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27347..27598
                     /locus_tag="OO230_001964"
     CDS             27347..27598
                     /locus_tag="OO230_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5691740.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27600..29696
                     /locus_tag="OO230_001965"
     CDS             27600..29696
                     /locus_tag="OO230_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5691741.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7291 a         7382 c         8062 g         7055 t
ORIGIN      
        1 catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga aaatctttaa
       61 atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga aaaccggctt
      121 caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc gtttgtatga
      181 cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg tgctgatctt
      241 tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg cggaaatcgt
      301 tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct ggtttaacaa
      361 cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca aaaccggcaa
      421 catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa tctacgaaag
      481 cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca tgctgggcgg
      541 tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg actacaacgt
      601 tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca acaaaatcat
      661 ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca ttggtaaaca
      721 tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg aagggctgaa
      781 aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga ttgaaaaata
      841 atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct caggcgagaa
      901 gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg tgcgaatgga
      961 tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg gcgcacacct
     1021 gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta cgcccgccat
     1081 gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg gtttgtttct
     1141 tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa tcttcacctt
     1201 cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca cgccagagca
     1261 tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact cagtaggtca
     1321 caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg gcgcattcag
     1381 cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact ttatttccga
     1441 aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt tgttgattac
     1501 gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg ggcgttttgc
     1561 agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg gcgatgaagt
     1621 ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact ggcggcgcac
     1681 ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga tttatacctg
     1741 gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca gcctgatgct
     1801 taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc tgctggcaca
     1861 tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag taatggcctg
     1921 tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca ccaccatttc
     1981 ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg acattcgctc
     2041 gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa gtactggcct
     2101 gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc tagcgacagt
     2161 gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag ccctcccgct
     2221 ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt aaagccgaac
     2281 cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt aatagcggac
     2341 cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct gccgcgctgc
     2401 tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc atgggggatg
     2461 aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa gctgtattgc
     2521 tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat attgccgaat
     2581 ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc gaagggcaga
     2641 atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc acccaggtgg
     2701 agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa catgggttgc
     2761 gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt gaagtggcct
     2821 tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt atccgtaaaa
     2881 ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca gagcaacgac
     2941 gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag ctatggcagg
     3001 actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa atcaggagaa
     3061 tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc tggcgcactg
     3121 gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg tggaacagtg
     3181 gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg atgaactgtc
     3241 gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt cgttagatat
     3301 tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga cgctgcaaac
     3361 agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga aaaacgccac
     3421 gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg actggctggt
     3481 tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca gacgggtgct
     3541 ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg cagaaaagct
     3601 gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg atgctgaaaa
     3661 agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg ttggcgcttc
     3721 cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg cgattaatca
     3781 tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg actggaaagt
     3841 ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg ctgccggatg
     3901 atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt taagcgtcgc
     3961 atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa ttcaattggt
     4021 tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta ttcgtggtgg
     4081 aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg cataccagcc
     4141 cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact ttgggcatgc
     4201 agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta tcaatgttgt
     4261 gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact tccgctcttg
     4321 ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc accagtttca
     4381 aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca tgaataaaag
     4441 catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag atattgccgt
     4501 acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg gcattatctt
     4561 taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata aattatcctt
     4621 attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg ccactgttgt
     4681 agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc gcgatagtca
     4741 atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc gttaatcaac
     4801 cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc atcagcaacg
     4861 cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc gccagatgcc
     4921 acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga acaggtttat
     4981 caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac gcccgatacc
     5041 tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca tcccatagca
     5101 ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg cggtgctggc
     5161 aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata tccatgccgc
     5221 gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc agccagccgt
     5281 taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga tgcgcaccat
     5341 actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct ttaaagcgag
     5401 tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg caaacaatcc
     5461 ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag tcatcacaga
     5521 ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa cgttcttcaa
     5581 tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct tcgccagcag
     5641 gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct gccagcacca
     5701 cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg ccgttttctt
     5761 catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt tcggcttcgg
     5821 caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg ccgggctgta
     5881 agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg tgagtgatgc
     5941 gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt gcatgggtat
     6001 ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg agtaacaaaa
     6061 cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata ccggcaccaa
     6121 taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa cacaacaccc
     6181 taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt cgccgcgata
     6241 gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg gcacaacggg
     6301 tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt ccagacctgg
     6361 aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg ccgctttcag
     6421 ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa tttccagtcg
     6481 caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga catgtggtaa
     6541 gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca taatttctct
     6601 acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat tatctaaaac
     6661 gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca tttgtttttc
     6721 attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt tagtcgattt
     6781 ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt aaccttacct
     6841 tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac tggaacataa
     6901 cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc ggagcaactg
     6961 gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg ggtttctggc
     7021 tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc agcgcccgcc
     7081 gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc gcgccgggtt
     7141 gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct ggtgaacgcg
     7201 ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt gacgtcaacg
     7261 caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct gttctccaaa
     7321 aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga tagctcttat
     7381 gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact ggggggtgaa
     7441 cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag cgagtggcgc
     7501 gcccgcgtgg tttatgcgct taaatcgcgt gcgcctgtcg cggcaccttc gcaatccgtc
     7561 gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc gccgctggtg
     7621 gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga cgttcgccat
     7681 atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc gctgggcgtc
     7741 tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg gctgacaggc
     7801 gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct acagtggcac
     7861 ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct tacccgcagt
     7921 gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc cgcgacgacg
     7981 ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc gctaattaat
     8041 ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc ggaagtcgag
     8101 aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg cgcccgtgcc
     8161 ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt ccgcgtattt
     8221 atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt gattatgatt
     8281 ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc cgctgacgaa
     8341 gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga cttcctgtat
     8401 caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga tctcgcctgg
     8461 tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca gggcgcggag
     8521 ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc taatcgcatg
     8581 gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg catggacacc
     8641 gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca gcgagatgtc
     8701 tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg acagacgccg
     8761 agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat ttaaacgacg
     8821 gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac ggcatgtatc
     8881 agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag ccgcgccacg
     8941 cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag tggcaggcga
     9001 tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc aaccgccaga
     9061 cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag atgctgcact
     9121 cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta ctctgcacct
     9181 cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag aagatttctg
     9241 aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag gaaaaagtcg
     9301 ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag ttcaaaacca
     9361 cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg aacttcgtgg
     9421 cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt gggctttcta
     9481 tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc tatctgccgc
     9541 tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac tggggtaacc
     9601 gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg gttgagacgt
     9661 ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc ccgtatgaat
     9721 ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac tggcacctga
     9781 cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg aaaactggcc
     9841 tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac cagaatctga
     9901 tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc aaagagagcg
     9961 ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg tcattcccga
    10021 cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc gacaacatcg
    10081 ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt gtaacaggct
    10141 gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt aaagcaccgg
    10201 gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca cggatgtata
    10261 aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata gggcgctggg
    10321 cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg ggcatcattc
    10381 gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg tcttgtaggc
    10441 ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc tatgtccaaa
    10501 ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc gctggcagaa
    10561 actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc gctggataat
    10621 ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt gagcctgcat
    10681 ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg ctatctgttc
    10741 ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa cctgaaagtg
    10801 taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact gtgggaacag
    10861 ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat gaaccgggcg
    10921 ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca atccggcagc
    10981 cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct gccgattatc
    11041 gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa atatcaccca
    11101 ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa atgggaaccc
    11161 ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg attacacgaa
    11221 gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg cgtttttatt
    11281 ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa caggtataga
    11341 atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc agatggacaa
    11401 aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc aattcggatg
    11461 ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt gtggcaatca
    11521 tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg ttatgtgagt
    11581 tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa tcgaagaagt
    11641 agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt ctcctgcacc
    11701 ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta ttatgaattt
    11761 caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat atatatcata
    11821 gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa aaatattgtg
    11881 actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat cgacgactat
    11941 caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat ggttaaaaga
    12001 aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa ggaacaggca
    12061 gctcgatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc ccgctctttt
    12121 caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa aaattacgaa
    12181 aaaatctctc attcaacgct gggctactgg ttgtggaatc attatttaag tgaatgtcag
    12241 gagttacttc cttcatcttc gctctctccg cgcaaactta ggcgagtgat agaaatgtgg
    12301 atgcccgtga cgacagggca tcatggtcgg ccccctgatc gtatggatga attggataat
    12361 tttctgcctg aagacaaagc tgcagcacga gattttctcc ttgaaatcaa gccactgttt
    12421 ccgcgaatag agatccccac attctgggat gatgacgagg gcgttgaact tataaaacaa
    12481 ctttcctggt atatctctgc aaccgtcgta cttgcagact ggacagggtc gtcaacgcga
    12541 ttttttccac gcgtcgcaca ggcaatggat attgaagatt actggcaaaa ggctttagtt
    12601 caggctcaaa acgcattaac cgtctttccg ccacaagcag aaaccgcgcc tttcaccgga
    12661 attaatacgc tgtttccttt tattgagaat acgacaccgt tacagcaaaa ggtgctggat
    12721 ctggatatca atcagccagg accacagttg tttattctgg aagatgtgac cggcgcaggt
    12781 aaaacagaag cggcgcttat cctggcgcac aggttgatgg ctgcgggtaa cgctcagggg
    12841 ctgttttttg gcctgccaac aatggcaacg gccaatgcca tgtacgatcg actggtcaaa
    12901 acctggcttg ctttctattc gccagaggtg ttccccgctc cagcggagat aaaccggcgc
    12961 aatacggtgc ggttttcccg gaagtaaacc ggacgcactg gatgcgatga tgggtatcac
    13021 ttagaattcc ccgcccctgc ggtagaactc ccagctccca ttttcaaacc catcaagacg
    13081 ccttcgccag ctccttcacc aacggtagca ttatccgcat aacgtcacgg cagcgacgtt
    13141 ctattcttcc aggaagtgcc ttatcaatat gttgttgatt atccagtctt acgtcatgcc
    13201 agctatttcc cgccgggaag gcaggtgttt ttgcgcgttg ctgataacca tccttatttc
    13261 caagattcca gttagtcgct tccaccgaaa gtacagcaat gcccgctttg tcgaatattt
    13321 ctgcgtcatt acaaccccca gtgcctttcg gataattttt attcaaaccc ggattggtcg
    13381 ttgcggctat tccgtgactg cgtgcaattg ccagcgccct gtcgcgcgtt aatttcctta
    13441 ctgcttcagg ggtttttaca ccgctgttga aatacaattt atcgccaaca attaagttat
    13501 cgagattaat caccagcagc gtatttttct tttcggtgtc actcatccgc ttgagtaaat
    13561 tctcagcgcc taattttcct tcctcttcgc cgctggtcgc cacaaatcga ataccatact
    13621 cggtaggcgt atttttcagg cgttctgcca gttccagcat gacacctaaa cctgcggcgt
    13681 tatcatctat tccttgtaac gtcagtccgc cgagattggc atctgcgtca gcatcgctca
    13741 gcggggcgta agtatccaga tgcgccataa tgatgatctg ctgcggcgct ttgccttcat
    13801 gagcggcaat caccgtactt cctgtcacgt tatgccagtt cttgcgatta tcgcgggcgg
    13861 tataaatata ccgactatta aatgtccgaa tatcactgcg ataacccatt tgctgaaact
    13921 gttggcgaat ataatcggca gataacattt ctgctggagt tccggtcatg cgtcccggaa
    13981 agaaagtagc aatatgtcgt gcctgggtat tagcaaaatc gccaggttta ggtgacgagg
    14041 cgtgtacggg gagaataaag catacgccga gcgccagggc agcggtacgg tggcgcaatg
    14101 cggaaaacat agtgagtcct taaataccat gcaaattttt ttaccgccat agtatgaaac
    14161 tgccgctgcg ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg tgccttaagc
    14221 actttttgat attagctttg ccaaatcgtt attccgttaa ggaactactc attctaattg
    14281 gtaatttcat tcgttctctt acgctcccta tagtcgaaac atctgatggc aagaaaatag
    14341 cggtattgca aaggaacggt tatggctcaa aaacgactta ctcacctgcg gcaactggag
    14401 gcggaaagca tccacattat tcgcgaggtg gcggcagaat tctcaaatcc ggtgatgctc
    14461 tactctatcg gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc gttttatcca
    14521 ggtacgctgc ctttcccgtt gctgcatgtc gataccggct ggaaattccg cgagatgtat
    14581 gagttccgcg atcgtacggc gaaagcctac ggctgcgagc tgctggtgca taaaaacccg
    14641 gaaggcgtgg cgatggggat taatccattc gtgcacggca gcgcgaaaca taccgatatt
    14701 atgaaaactg aaggcctgaa acaggcgctg aacaaatacg gttttgatgc cgccttcggt
    14761 ggtgcgcgcc gtgacgaaga gaaatcccgc gctaaagagc gaatctactc tttccgtgac
    14821 cgcttccatc gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa ctacaacggg
    14881 caaattaaca aaggcgaaag catccgcgtc ttcccgctct ctaactggac cgagcaggat
    14941 atctggcaat acatctggct ggaaaatatc gacattgtgc cgctgtatct cgctgcggaa
    15001 cgtccggttc tggaacgcga cggtatgttg atgatgattg atgacaaccg tatcgacctg
    15061 caaccgggcg aagtgatcaa aaaacggatg gtgcgtttcc gtacgctggg ctgctggccg
    15121 ctgaccggtg cggtggagtc aaatgcacaa acactgccgg aaatcatcga agagatgctg
    15181 gtttccacca ccagtgaacg tcagggtcgc gtgattgacc gcgaccaggc gggatccatg
    15241 gagctgaaaa aacgtcaggg gtatttttaa gatgaacacc gtacttgcac aacaaatcgc
    15301 caatgaaggc ggcgtcgaag cctggatgat tgcgcaacaa cataaaagcc tgctgcgttt
    15361 tctgacctgt ggtagcgtcg atgacggcaa aagtaccctg attggtcgtc tgctgcacga
    15421 tacccgccaa atctacgaag atcagctctc atcgctgcat aacgacagta agcgtcacgg
    15481 cacccagggc gaaaagctgg atctggctct gctggtggac ggcctgcaag ctgagcgcga
    15541 acagggcatc actattgatg tggcctaccg ctatttctct accgagaagc gtaaatttat
    15601 tatcgccgac accccagggc acgagcagta cactcgcaat atggcgactg gcgcatcgac
    15661 atgtgaactg gcgatcttac tgattgatgc ccgtaaaggc gtgctcgatc aaacccgtcg
    15721 tcacagtttt atctccacac tgttggggat caaacatctg gtcgtggcga tcaacaaaat
    15781 ggatctggtg gattacagtg aagagacgtt cacccgtatt cgtgaagatt atctgacctt
    15841 tgccgggcag ctgccgggta atctggatat ccgctttgtg ccgctctccg cactggaagg
    15901 tgacaacgtg gcttcgcaaa gtgaaagtat gccgtggtac agcggtctga cactgctcga
    15961 agtgctggaa accgtggaga tccagcgagt ggtggatgcc cagccaatgc gcttcccggt
    16021 gcagtacgtt aaccgcccaa atctcgattt tcgtggctac gccggaatgc tggcatccgg
    16081 tcgcgtggaa gtcgggcaac gtgtaaaagt gctgccctct ggtgtggaat caaacgtcgc
    16141 gcggatcgtg acttttgatg gtgatcgcga agaagtcttt gccggagaag cgatcaccct
    16201 ggtgctgacg gatgagatcg atatcagccg tggtgatctg ctgctggcgg cagacgaagc
    16261 gttaccggcg gtgcagagtg cgtcggtgga tgtggtatgg atggcggaac agccgctttc
    16321 cccgggccag agttatgaca tcaaaattgc cggtaagaag acgcgtgctc gtgttgatgg
    16381 cattcgttat caggttgata ttaataacct tacccaacgc gaagttgaaa acctgccgct
    16441 gaacggcatc ggcctggtgg atctcacttt tgacgagcca ctggtgttag atcgctatca
    16501 gcaaaatccg gttaccggtg ggctgatttt tatcgatcgc ctgagcaatg tgaccgtggg
    16561 tgccggtatg gtgcacgagc cagttagcca ggcaacagct gcgtcatctg aattcagtgc
    16621 attcgaactg gaattgaatg ccctggttcg tcgccacttt ccgcactggg gcgcgcgcga
    16681 tttgctgggg gataaataat ggcgctgcat gacgaaaacg tcgtctggca tagtcatccg
    16741 gtcactgtgc aacaacgcga actacaccac ggtcatcgtg gtgtagtgct gtggtttacc
    16801 ggcctctccg ggtccggtaa atcaacggtc gccggggcgc tggaggaggc gttacataaa
    16861 ctcagcgtca gtacgtatct gctggatggc gacaatgttc gccacggatt atgcagcgat
    16921 ctcggtttta gcgatgccga tcgtaaagag aatatccgtc gcgtcggtga agtggcgaat
    16981 ttgatggttg aagccggact ggtggtgctg accgcattta tctcgccaca ccgcgccgaa
    17041 cgccagatgg ttcgcgaacg cgtaggagaa gggcgcttta ttgaagtgtt tgtcgatacg
    17101 ccgctggcga tttgcgaagc ccgcgatcca aaaggcttat ataagaaagc gcgtgccggt
    17161 gaactgcgca actttacggg aatagattcc gtttacgaag cgcctgaatc ggcagaaatt
    17221 catctcaatg gtgaacaatt agtaacaaat ttggtacagc aattattaga tctgttgaga
    17281 cagaacgata ttatcagatc ctgagacacc accgggtttc catgcccggt tcgtcaaagt
    17341 aacaggatca aatatgcgta atagccataa cattacacta acaaataacg acagccttac
    17401 tgaggatgaa gaaactacat ggtcactgcc tggtgccgtg gtcggtttta tctcctggtt
    17461 atttgcgctg gcgatgccga tgttgattta tggctctaac acgctgttct tctttctcta
    17521 cacctggcct ttctttctgg cactgatgcc tgtcgcggta gtggtgggga ttgcgctgca
    17581 ttcattgatg gacggaaagc tacgctacag tattgttttc accctggtga cagtaggcat
    17641 tatgtttggc gcactgttta tgtggctact gggctaattt gtactttctt gtctcctctg
    17701 ttcataattc aaaccgtaac taataatgag attatgttct gcacgccctg ggtatacgta
    17761 acaatgggca aatgtggtac atttgcccgc gttgtcgcgg tatccccaac agaggatgta
    17821 gagtcgtctt cggatgcatg ggatgatgat gcagtttttc agggggcagg atgggtaaac
    17881 taacgctgct gttgctggct attctggtct ggctacagta ttcgctgtgg ttcggtaaga
    17941 acggtataca tgactatacc cgcgtcaatg atgatgtggc ggcactgcaa gctacaaacg
    18001 cgaaacttaa agcgcgaaac gatcaacttt ttgccgaaat tgacgatctc aatggcggcc
    18061 aggaggcgct cgaagagcgt gcgcgtaatg aactcagcat gaccaggccg ggcgaaactt
    18121 tttatcgtct ggtgcctgac gcgtcgaagc gcgcacagtc tgcggggcaa aacaatcgat
    18181 aaatcagccc gggaattaac atggcaacca ctcatttgga tgtttgcgcc gtggttccgg
    18241 cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc tcaatcggta
    18301 atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg gtgacacgtg
    18361 tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg gcgaatcatc
    18421 cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg gcaggtctga
    18481 aagccgctgg cgatgtgcag tgggtattgg tgcatgacgc cgctcgtcct tgtttgcatc
    18541 aggatgacct tgcgcgattg ttggcgttga gcgaaaccag ccgcacggga gggatcctcg
    18601 ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc attgctcata
    18661 ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct cgtgagctgt
    18721 tacatgactg tctgacgcgc gctctaaatg aaggcgctac cattaccgac gaagcctcgg
    18781 cgctggaata ttgcggattt catccgcagt tggtcgaagg ccgcgcggat aacattaaag
    18841 tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc atccatcagg
    18901 agaatacata atgcgaattg gacacggttt tgacgtacac gcctttggcg gtgaaggccc
    18961 aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc attctgatgg
    19021 cgacgtggcg cttcatgcgt tgaccgatgc attgcttggc gcggcggcgc tgggggatat
    19081 cggcaaactg ttcccggata ccgatccggc atttaaaggt gccgacagcc gcgagctgct
    19141 acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg tcgatgtcac
    19201 tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg tgtttattgc
    19261 cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta cggaaaaact
    19321 tggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac tcattaaggc
    19381 aacaaaatga ttgagtttga taatctcact tacctccacg gtaaaccgca aggcaccggg
    19441 ctgctgaaag ccaatccgga agactttgtg gtggtggaag atttgggctt tgagcctgat
    19501 ggtgaaggtg agcatattct ggttagaatc ctcaaaaacg gctgcaatac ccgttttgtg
    19561 gcggatgcac tggcgaaatt cctgaaaatt cattcccgtg aagtcagctt cgctgggcaa
    19621 aaagacaaac atgctgtaac ggaacagtgg ttatgtgctc gcgtgccggg caaggaaatg
    19681 cccgatctga gcgcctttca actggaaggc tgccaggtgc tggagtatgc gcggcacaag
    19741 cgcaagctgc gtttaggggc gctgaaaggt aacgccttta ccctggttct gcgcgaagtg
    19801 agcaatcgcg atgacgttga acaacgtctg atcgatattt gcgtaaaagg tgtaccgaac
    19861 tacttcggtg cccaacgttt tgggattggc ggtagcaact tgcagggcgc gctgcgctgg
    19921 gcgcaaacca atactccggt gcgcgatcgc aataaacgga gtttttggtt gtcggcagcc
    19981 cgcagtgcgt tgtttaatca gattgttgct gagcgcctca aaaaagcaga cgttaatcaa
    20041 gttgttgacg gcgatgcgct acaattagcc ggacgtggta gctggtttgt agcaaccacc
    20101 gaagaactgg cggaattaca gcgtcgcgtc aacgataaag agctgatgat aaccgccgca
    20161 ttgccgggca gtggcgaatg gggaactcag cgtgaagcgc tggcattcga acaagcagct
    20221 gtcgccgcag aaactgaatt acaagcttta ctggtgcgcg aaaaagttga agccgcgcgc
    20281 agagcgatgc tgctgtatcc gcaacaatta agctggaact ggtgggatga cgtcaccgta
    20341 gagatccgtt tctggcttcc ggcgggtagt tttgcaacca gcgttgtcag ggaacttatc
    20401 aacacaacag gtgattatgc gcatattgct gagtaattat gacggggtac atgcacccgg
    20461 tatacaaacg ctggcgaaag ccttgcgtga gtttgctgac gttcaggtgg tcgcccccga
    20521 tcgtaaccgc agcggcgctt caaattctct gacactggaa tcctccctgc gcacgtttac
    20581 ctttgaaaat ggtgatattg ctgtgcaaat gggaaccccg accgattgtg tctatcttgg
    20641 cgtgaatgct ctgatgcgtc cgcgcccgga cattgttgtg tccggaatta acgccgggcc
    20701 gaatctgggg gatgatgtta tttattccgg tacggtagcc gccgcgatgg aaggccgtca
    20761 tttaggtttt ccggcgctgg ccgtctcgct tgacgggcat aaacattacg acactgccgc
    20821 ggcggtaacc tgttcaattt tgcgcgcact gtgtaaagag ccgctgcgca ccggacgtat
    20881 tcttaatatt aacgttcagg atttaccctt ggatcaaatc aaaggtattc gcgtgacgcg
    20941 ctgcggtaca cgacatccgg cagatcaggt gatcccgcag caagatccgc gcggcaatac
    21001 gctgtactgg attggcccgc cgggcggtaa atgtgatgcc ggtccgggga ccgattttgc
    21061 tgcggtagat gagggctatg tctccatcac gccgctgcat gtggatttaa ctgcgcatag
    21121 cgcgcaagat gtggtttcag actggttaaa cagcgtggga gttggcacgc aatggtaagc
    21181 agacgcgtac aagcacttct ggatcaatta cgtgcgcaag gtattcagga tgagcaggtg
    21241 ctgaatgcac ttgccgccgt gccgcgtgaa aaattcgttg atgaagcgtt tgaacaaaaa
    21301 gcctgggaca atatcgcctt gccgataggt caggggcaga caatttcgca gccgtatatg
    21361 gtggcgcgaa tgaccgaatt actcgagctg acgccgcagt cgcgggtgct ggaaattggc
    21421 actggttcgg gatatcaaac ggcaatcctg gcgcatcttg tccagcatgt ttgctcggtt
    21481 gaacggatta aaggcttgca gtggcaggca cgtcgccgcc tgaaaaatct tgatttacat
    21541 aatgtttcaa cccgtcatgg tgatggatgg caaggttggc aggcacgtgc gccgtttgac
    21601 gctatcattg ttacggcggc accgccggaa attccaactg cgctaatgac gcagctggac
    21661 gaaggcggga ttctcgtctt acccgtaggg gaggagcacc agtatttgaa acgggtgcgt
    21721 cgtcggggag gcgaatttat tatcgatacc gtggaggccg tgcgctttgt ccctttagtg
    21781 aagggtgagc tggcttaaaa cgtgaggaaa tacctggatt tttcctggtt attttgccgt
    21841 aggtcagcgt atcgtgaaca tcttttccag tgttcagtag ggtgccttgc acggtaatta
    21901 tgtcactggt tattaaccaa tttttcctgg gggataaatg agcgcgggaa gcccaaaatt
    21961 caccgttcgc cgcattgcgg ctttgtcact ggtttcgcta tggctggcag gctgttctga
    22021 cacttcaaat ccaccggcac cggtcagctc cgttaatggc aatgcgcctg caaatactaa
    22081 ttctggtatg ttgattacgc cgccgccgaa aatggggacg acgtctacag cgcagcaacc
    22141 gcaaattcag ccggtacagc agccacaaat tcaggctact caacaaccgc aaatccagcc
    22201 agtgcagcca gtagctcagc agccggtaca gatggaaaac ggacgcatcg tctataaccg
    22261 tcagtatggg aacattccga aaggcagtta tagcggcagt acctataccg tgaaaaaagg
    22321 cgacacactt ttctatatcg cctggattac tggcaacgat ttccgtgacc ttgctcagcg
    22381 caacaatatt caggcaccat acgcgctgaa cgttggtcag accttgcagg tgggtaatgc
    22441 ttccggtacg ccaatcactg gcggaaatgc cattacccag gccgacgcag cagagcaagg
    22501 agttgtgatc aagcctgcac aaaattccac cgttgctgtt gcgtcgcaac cgacaattac
    22561 gtattctgag tcttcgggtg aacagagtgc taacaaaatg ttgccgaaca acaagccaac
    22621 tgcgaccacg gtcacagcgc ctgtaacggt accaacagca agcacaaccg agccgactgt
    22681 cagcagtaca tcaaccagta cgcctatctc cacctggcgc tggccgactg agggcaaagt
    22741 gatcgaaacc tttggcgctt ctgagggggg caacaagggg attgatatcg caggcagcaa
    22801 aggacaggca attatcgcga ccgcagatgg ccgcgttgtt tatgctggta acgcgctgcg
    22861 cggctacggt aatctgatta tcatcaaaca taatgatgat tacctgagtg cctacgccca
    22921 taacgacaca atgctggtcc gggaacaaca agaagtgaag gcggggcaaa aaatagcaac
    22981 catgggtagc accggaacca gttcaacacg cttgcatttt gaaattcgtt acaaggggaa
    23041 atccgtaaac ccgctgcgtt atttgccgca gcgataaatc ggcggaacca ggcttttgct
    23101 tgaatgttcc gtcaagggat cacgggtagg agccacctta tgagtcagaa tacgctgaaa
    23161 gttcatgatt taaatgaaga tgcggaattt gatgagaacg gagttgaggt ttttgacgaa
    23221 aaggccttag tagaagagga acccagtgat aacgatttgg ccgaagagga actgttatcg
    23281 cagggagcca cacagcgtgt gttggacgcg actcagcttt accttggtga gattggttat
    23341 tcaccactgt taacggccga agaagaagtt tattttgcgc gtcgcgcact gcgtggagat
    23401 gtcgcctctc gccgccggat gatcgagagt aacttgcgtc tggtggtaaa aattgcccgc
    23461 cgttatggca atcgtggtct ggcgttgctg gaccttatcg aagagggcaa cttggggctg
    23521 atccgcgcgg tagagaagtt tgacccggaa cgtggtttcc gcttctcaac atacgcaacc
    23581 tggtggattc gccagacgat tgaacgggcg attatgaacc aaacccgtac tattcgtttg
    23641 ccgattcaca tcgtaaagga gctgaacgtt tacctgcgaa ccgcacgtga gttgtcccat
    23701 aagctggacc atgaaccaag tgcggaagag atcgcagagc aactggataa gccagttgat
    23761 gacgtcagcc gtatgcttcg tcttaacgag cgcattacct cggtagacac cccgctgggt
    23821 ggtgattccg aaaaagcgtt gctggacatc ctggccgatg aaaaagagaa cggtccggaa
    23881 gataccacgc aagatgacga tatgaagcag agcatcgtca aatggctgtt cgagctgaac
    23941 gccaaacagc gtgaagtact ggcacgtcga ttcggtttgc tggggtatga agcggcaaca
    24001 ctggaagatg taggtcgtga aattggcctc acccgtgaac gtgttcgcca gattcaggtt
    24061 gaaggcctgc gccgtttgcg cgaaatcctg caaacgcagg ggctgaatat cgaagcgctg
    24121 ttccgcaagt aagtaagcat ctgtcagaaa ggccagtctc aagcgaggct ggcctttttc
    24181 ttttgtttgg gaagcttgtg tttttgtcgc ctggataaga cacgtcagcg tcctacccgg
    24241 caatcagcat tctatgtgct catcatcttg cgcaccaact gcataaacag ctcctgctct
    24301 tctccactca aacgccccaa aaattcctca tccacgctgt caccaatagg tatcgccgca
    24361 gcaagtagct tttctccttc ggcagtcagc cagacaaagc gtcgccgctt gtcagcgacg
    24421 tcatgttcac gtctgactaa gccacgattt tccattcttg ccaacatttc tgctagcgtt
    24481 gctttggtgc tgactgctgc ttctatcagt gccacctgtt caataccagg tttatcagca
    24541 atggcgcgca tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg ctgccagcga
    24601 gccgtgtgct gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg taacgccatg
    24661 taaactccgg gctaattctc tgtcgctatc atagcggttt tttagtgacg aagaagatgt
    24721 tgttactttt caatccttgc cgctgtggaa atgaaggagt atgttaataa taacgttcgc
    24781 atacgaataa ttaagagaat aagcaatgaa actgatcgtc gggatgacag gggctaccgg
    24841 tgcgcctctt ggtgtggcat tactgcaagc gctgcgtgag atgccgaatg tcgagactca
    24901 tctggtgatg tcgaagtggg cgaaaaccac cattgaactg gaaacgcctt acagcgctcg
    24961 cgatgttgct gccctcgcag acttcagcca taacccggcg gatcaggcgg cgatcatctc
    25021 atccggttct tttcgtaccg acggcatgat cgttattccg tgcagtatga aaacgctcgc
    25081 cggtatccgc gctggttacg ccgatggcct ggtagggcgc gcggcggacg tcgtgctcaa
    25141 agaaggccgc aaactggtgc tggtgccgcg tgaaatgccg cttagcacca tccatctcga
    25201 aaatatgctc gcactttcac gcatgggcgt ggcgatggtg ccgccgatgc ctgcctttta
    25261 taaccatccc gaaacggtag atgacattgt ccaccatgtg gtagcccgcg tgctggatca
    25321 atttggcctc gaacatcccc acgccaggcg ctggcaagga ttgccgcagg cccggaattt
    25381 ttctcaggag aatgaataat ggcatttgat gatttacgca gctttttaca ggcgcttgat
    25441 gaccacggcc agttactgaa aatcagcgaa gaagtgaacg ctgagccgga tctggcagca
    25501 gcagctaacg ccaccgggcg tatcggcgac ggcgcgcccg cgctgtggtt tgataatatt
    25561 cgcggcttta ccgatgcccg cgtggcgatg aataccatcg gttcctggca gaaccacgcg
    25621 atttcccttg gcctgccacc aaacaccccg gtcaaaaagc agattgatga gtttatccgc
    25681 cgctgggata acttcccgat tgccccggag cgccgcgcca atccagcctg ggcgcagaac
    25741 accgtcgatg gcgaagagat caacctgttc gatattctgc cgctgtttcg cctgaatgat
    25801 ggcgatggcg gtttctatct cgacaaagcg tgcgtggttt cccgcgatcc gctcgacccg
    25861 gataacttcg gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg caagcgtaag
    25921 ctcggcctgc aaccggtgcc gatgcacgat atcgccctgc atctgcataa agcagaagag
    25981 cgcggtgaag atctgccgat tgcgatcacg ctcggtaacg atccgatcat cacgctgatg
    26041 ggggccacgc cgctgaaata cgatcagtct gagtatgaaa tggcaggcgc gctgcgtgaa
    26101 agcccgtacc cgatcgccac cgctccgctg accggttttg atgtgccgtg gggttcagaa
    26161 gtgatcctcg aaggggttat cgaaagccgt aaacgcgaaa tcgaagggcc gttcggtgag
    26221 tttaccgggc actactccgg tgggcgcaac atgaccgtgg tgcgcatcga taaagtctct
    26281 tatcgcagca aaccgatttt cgaatcactc tatctcggta tgccgtggac cgaaatcgac
    26341 tacctatgac taggtaattt attcaagata accgggaaga gattaaaaaa gacgggaata
    26401 gattggaatc aacaagttag gaactcaagg ttttcaagat aaagcgagtc caaaaatgtt
    26461 aaacggttcg atatcaataa aacattcaag ataactcaca ggttcttaca atagccttta
    26521 aagaccgttc aaaaacgatt caggtgttta ttatagcgct gatgggtgca agcgccaaaa
    26581 ccaatgtaat tttagcacag ctaggcactt gcactgggtt atgcgctggc tgctttctga
    26641 ggtagattta agtgatcgcc cttagcttcg gacaacgcgt aaatctctct aaccttttcg
    26701 tctggcaaat taccaaggct taacgctatg gctctacgaa ctccaactag agcgtgcaac
    26761 tcttggatgt gctcatccag aagaatgagt aattgctccg ctccttttct cccaagaaca
    26821 tcagctactt gttgtttttc ttgtggttta agaaatgaca agaacattag aatttgagca
    26881 tcaacattat ccgcattctc ttttttgggt tctttggaat cttttagttc aacatcaccc
    26941 tctcccgtca tgagccattc caaggtaaca ttttcgcttt ctgctatctt ttttaactga
    27001 tacgggcgtg gtatcggtgc gtaatcccgc ctgttgtaat aattttttaa agtgccttca
    27061 ttgatccccc atgcccgagc agccgcattt cttgaagggt atcgttctac taacctaaag
    27121 attctttcga taatcggttc ttttccatcg gcaggaaaag aaccttgggg tgcttttctc
    27181 attcttggtt cctttctatc taattcattg ataaataata aaattaaaaa ataaaccata
    27241 aaaacaccaa agaacccttt gcagggaatc tttggtatct taatctttat ccatacggat
    27301 atccgaccgg atatccgcgc gggtaatttt gtaggttaaa ggaaagatga gacaaagaga
    27361 agatagtgac cgcgattggc cttcgctgaa gattcagctg gcgctcaaag ataacgggtg
    27421 gtcttccttc cgggagcttt cactccatgc tggttatgct caggattcac tgaaagcggt
    27481 agttcgcaca ccaaacaaaa aatacgaacg cgtaatttct gacgctatag gtgtaccgcc
    27541 agaaatcatc tggcccagcc ggtatcgtaa cgatggattt atcaggaagg cgtcgtgata
    27601 tgcagtcgta cgttaccgta aatgacctgc tcggcgtacc aggaatgcca gccaccacaa
    27661 aaggcatccg acaggcgtta cagcgttttt ctcgtgatct tggtgacgtt agtcgccgtc
    27721 gggaaggcac aaaagccatt gagtaccaca ttgattgtct gcctgaaata acacgaaagg
    27781 cactgcgtga acgctatgtg gaacagctgg tggctacaga gaacaatgtt tctgaagtga
    27841 aagctgttac cagaaaaaca cgcaatcctg acgctgtcca ggcaatcgaa gcatatcgcg
    27901 gttctccaca actgatggaa gaacgcctga atgcgctgac cgaaaaccag cgctgggtat
    27961 ctgaagcaag agctgcgctg gtggtggaag tgctgaagct ggaaagcgcc ggtaaccccg
    28021 ggcgactgaa agccattaac tttcttgttg aaaaagcccg taaaggtgag ctgccggagc
    28081 gcctgcaaca ggccgcagtt aacgccaatg caaaacgtgg cgctaatcgt actatcagcc
    28141 gcgatccact ttaccagtgg gttctgaaat acaaccagtc gcagaacgcc gccgaacggc
    28201 tgctgttact ggcaccggga aaacgcgacg aaataaaacc ggaagaaatc agctggctgc
    28261 ctgaatttct ggcgcagtat cgccaggtga acggcagacc catgtcagaa gcctacgagg
    28321 attttgtcgc agaatggcag cgtcgccatg cggacgaacc gtacatgctg gaggtgatgc
    28381 cttcctatga tgttgtccgg tatgccatga aaaaactgcc ggaagtggtg aaacaaaaag
    28441 ggcgcgttac cggcagtgaa taccgtcagc ttgaaggatt cacccgccgc gactggacag
    28501 ctatgccggt gaattatgtc tggattggtg acggtcacgg tatgaagctg aaatgtgcgc
    28561 atccgatcca cgggcgtcca ttctcaccgg aagtcacgtt tgtgatcgat ggtggaacac
    28621 ggtttgttgt tggctggagt cttgaccttg ccgaaaacgt gttcgccgtt gccggtgcga
    28681 tacagcacgg cattcgcaac cacggaaaac cgttcctgta ttactcggat aacggttccg
    28741 gtgaaacggc tgacatgctg gacaaagagg tcgtgggtat tctgccacgc ctggggatta
    28801 aacaccctac cggtattgcc ggtaatccac agggacgcgg cattattgaa cgcctgaacc
    28861 ggacactgcc gatgcgtata gccaggaggt atcgcaccta ttttggcaag ggtgctgacc
    28921 gggaatcgtt gcgtgtactt aaccgtgatc tgcgttcagc gtttaacgcc ctgcaacagg
    28981 acaagcctct gaacgaccgc cagaaagcgg caatgcgcga gctgccatcg tgggcggagc
    29041 ttattgaggc tattcgtgaa ggcgtggaat ggtacaacaa ccgcccgcat tctgagctgc
    29101 cgatgaaacc gaacggacgg cattacagcc cgacagagtt cagaaaaaaa cgccaggcag
    29161 aagaagacac cgaaattgag tggctgagtg atcttgagct gcgcgacatg ttccgcccaa
    29221 tggtggaacg accggtaaga cgctgcgaaa ttcagtggct gaacaatatt tactacgcgc
    29281 cggagttgcg tgacgaacac ggtcgcaaag tgcttatcag ttatgacatt catgacgccg
    29341 agcgaattac ggtacgtcgc aaggacggca gctttatctg tgaggcgata tggaacggca
    29401 ataaacgcgc agcctttgct gtcagtgctg aatatcacaa acaacagcag cgtattaaag
    29461 gaatgcgcaa acgcgctgag gaaaaaattc gtgatgctga agatgaaggt attcagattc
    29521 tggaacacaa acaggctgaa ccctggctga gtaatgttta tcgcccggtt ggtaatgtgg
    29581 tggcagttca gcaaccggaa tatgaagaag agcatgacga agaatttgaa cgcgatttcc
    29641 ggctggggat gcaaaaactt tttgccatgc aggaagaaga tgatccgctg gcctgaataa
    29701 aaaaacagtc cgcggggacg gactgttaaa taaaggaaac aaagctgatt tgaaataacc
    29761 gaggaaatac tatgactgat attgatgatg
//
LOCUS       DAJTPG010000042        29476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTPG010000042 DAJTPG010000000
VERSION     DAJTPG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(1..441)
                     /locus_tag="OO230_001966"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO230_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO230_001967"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO230_001967"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5691742.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO230_001968"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO230_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5691743.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO230_001969"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO230_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5691744.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO230_001970"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO230_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO230_001971"
     CDS             5754..6257
                     /locus_tag="OO230_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5691745.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO230_001972"
     CDS             complement(6337..6588)
                     /locus_tag="OO230_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5691746.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO230_001973"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO230_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5691747.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO230_001974"
     CDS             7052..7375
                     /locus_tag="OO230_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5691748.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO230_001975"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO230_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5691749.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO230_001976"
     CDS             complement(8080..8319)
                     /locus_tag="OO230_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5691750.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO230_001977"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO230_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5691751.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO230_001978"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO230_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5691752.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO230_001979"
     tRNA            complement(10643..10729)
                     /locus_tag="OO230_001979"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO230_001980"
     tRNA            complement(10742..10815)
                     /locus_tag="OO230_001980"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO230_001981"
     tRNA            complement(10870..10945)
                     /locus_tag="OO230_001981"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO230_001982"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO230_001982"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5691753.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO230_001983"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO230_001983"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5691754.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO230_001984"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO230_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5691755.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO230_001985"
     CDS             14483..14659
                     /locus_tag="OO230_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691756.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO230_001986"
     CDS             14646..14870
                     /locus_tag="OO230_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5691757.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO230_001987"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO230_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO230_001988"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO230_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5691758.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO230_001989"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO230_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5691759.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO230_001990"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO230_001990"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5691760.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO230_001991"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO230_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5691761.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO230_001992"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO230_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5691762.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO230_001993"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO230_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO230_001994"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO230_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5691763.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO230_001995"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO230_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5691764.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO230_001996"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO230_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5691765.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO230_001997"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO230_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5691766.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO230_001998"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO230_001998"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5691767.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO230_001999"
     CDS             complement(26368..26781)
                     /locus_tag="OO230_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5691768.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO230_002000"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO230_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5691769.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO230_002001"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO230_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5691770.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO230_002002"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO230_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..>29476)
                     /locus_tag="OO230_002003"
     CDS             complement(29220..>29476)
                     /locus_tag="OO230_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5691771.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7338 a         7613 c         7017 g         7508 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcaactcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccac
//
LOCUS       DAJTPG010000043        29447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTPG010000043 DAJTPG010000000
VERSION     DAJTPG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            61..342
                     /locus_tag="OO230_002004"
     CDS             61..342
                     /locus_tag="OO230_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5691772.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            308..1385
                     /locus_tag="OO230_002005"
                     /pseudo
     CDS             308..1385
                     /locus_tag="OO230_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1778..2119)
                     /locus_tag="OO230_002006"
     CDS             complement(1778..2119)
                     /locus_tag="OO230_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5691773.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2132..3004)
                     /gene="copD"
                     /locus_tag="OO230_002007"
                     /pseudo
     CDS             complement(2132..3004)
                     /gene="copD"
                     /locus_tag="OO230_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3008..3382)
                     /gene="yobA"
                     /locus_tag="OO230_002008"
     CDS             complement(3008..3382)
                     /gene="yobA"
                     /locus_tag="OO230_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5691774.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3521..3751
                     /gene="holE"
                     /locus_tag="OO230_002009"
     CDS             3521..3751
                     /gene="holE"
                     /locus_tag="OO230_002009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5691775.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3853..4509
                     /locus_tag="OO230_002010"
                     /pseudo
     CDS             3853..4509
                     /locus_tag="OO230_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4533..5195
                     /gene="exoX"
                     /locus_tag="OO230_002011"
     CDS             4533..5195
                     /gene="exoX"
                     /locus_tag="OO230_002011"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5691776.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5192..7252)
                     /gene="ptrB"
                     /locus_tag="OO230_002012"
     CDS             complement(5192..7252)
                     /gene="ptrB"
                     /locus_tag="OO230_002012"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5691777.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7462..8121)
                     /locus_tag="OO230_002013"
     CDS             complement(7462..8121)
                     /locus_tag="OO230_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5691778.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8448..8804)
                     /locus_tag="OO230_002014"
     CDS             complement(8448..8804)
                     /locus_tag="OO230_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5691779.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8871..9161)
                     /gene="yebG"
                     /locus_tag="OO230_002015"
                     /pseudo
     CDS             complement(8871..9161)
                     /gene="yebG"
                     /locus_tag="OO230_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9295..10473
                     /gene="purT"
                     /locus_tag="OO230_002016"
     CDS             9295..10473
                     /gene="purT"
                     /locus_tag="OO230_002016"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5691780.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10529..11170)
                     /gene="kdgA"
                     /locus_tag="OO230_002017"
                     /gene_synonym="eda"
     CDS             complement(10529..11170)
                     /gene="kdgA"
                     /locus_tag="OO230_002017"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5691781.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11207..13018)
                     /gene="edd"
                     /locus_tag="OO230_002018"
     CDS             complement(11207..13018)
                     /gene="edd"
                     /locus_tag="OO230_002018"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5691782.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13253..14728)
                     /gene="zwf"
                     /locus_tag="OO230_002019"
     CDS             complement(13253..14728)
                     /gene="zwf"
                     /locus_tag="OO230_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5691783.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15066..15935
                     /gene="hexR"
                     /locus_tag="OO230_002020"
                     /gene_synonym="yebK"
     CDS             15066..15935
                     /gene="hexR"
                     /locus_tag="OO230_002020"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5691784.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16063..17505
                     /gene="pyk"
                     /locus_tag="OO230_002021"
     CDS             16063..17505
                     /gene="pyk"
                     /locus_tag="OO230_002021"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5691785.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17636..18607)
                     /gene="lpxM"
                     /locus_tag="OO230_002022"
                     /gene_synonym="msbB"
     CDS             complement(17636..18607)
                     /gene="lpxM"
                     /locus_tag="OO230_002022"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5691786.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18727..20049)
                     /gene="mepM"
                     /locus_tag="OO230_002023"
     CDS             complement(18727..20049)
                     /gene="mepM"
                     /locus_tag="OO230_002023"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5691787.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20065..20997)
                     /gene="znuA"
                     /locus_tag="OO230_002024"
     CDS             complement(20065..20997)
                     /gene="znuA"
                     /locus_tag="OO230_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5691788.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21076..21831
                     /gene="znuC"
                     /locus_tag="OO230_002025"
     CDS             21076..21831
                     /gene="znuC"
                     /locus_tag="OO230_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5691789.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21828..22613
                     /gene="znuB"
                     /locus_tag="OO230_002026"
     CDS             21828..22613
                     /gene="znuB"
                     /locus_tag="OO230_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5691790.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22760..23770)
                     /gene="ruvB"
                     /locus_tag="OO230_002027"
     CDS             complement(22760..23770)
                     /gene="ruvB"
                     /locus_tag="OO230_002027"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5691791.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23779..24390)
                     /gene="ruvA"
                     /locus_tag="OO230_002028"
     CDS             complement(23779..24390)
                     /gene="ruvA"
                     /locus_tag="OO230_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5691792.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24529..24594)
                     /gene="yobI"
                     /locus_tag="OO230_002029"
     CDS             complement(24529..24594)
                     /gene="yobI"
                     /locus_tag="OO230_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5691793.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24664..25266
                     /locus_tag="OO230_002030"
     CDS             24664..25266
                     /locus_tag="OO230_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5691794.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25268..25789)
                     /gene="ruvC"
                     /locus_tag="OO230_002031"
     CDS             complement(25268..25789)
                     /gene="ruvC"
                     /locus_tag="OO230_002031"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5691795.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25824..26564)
                     /locus_tag="OO230_002032"
     CDS             complement(25824..26564)
                     /locus_tag="OO230_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5691796.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26593..27045)
                     /gene="nudB"
                     /locus_tag="OO230_002033"
     CDS             complement(26593..27045)
                     /gene="nudB"
                     /locus_tag="OO230_002033"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5691797.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27163..28935)
                     /gene="aspS"
                     /locus_tag="OO230_002034"
     CDS             complement(27163..28935)
                     /gene="aspS"
                     /locus_tag="OO230_002034"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5691798.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29245..>29447
                     /locus_tag="OO230_002035"
     CDS             29245..>29447
                     /locus_tag="OO230_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5691799.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
BASE COUNT         7219 a         7566 c         7232 g         7430 t
ORIGIN      
        1 caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag aggtatggca
       61 atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa cccaccatca
      121 atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc tgaactacac
      181 gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa ggttgatacc
      241 gatataacac accatcagcc taatgggcga caagggagag acagtccgtt aatggagaag
      301 ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa catatcgaag
      361 gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta cactaggtaa
      421 aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata tatacaaaaa
      481 ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta ctatggcagg
      541 ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa atacttataa
      601 aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt tactgacaga
      661 aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag ggaaaaacac
      721 gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca ttgttgaagg
      781 acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag ttacaagaga
      841 acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac ttccggcatg
      901 gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag acataacgaa
      961 tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta agacaggaat
     1021 gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac ttggtgcagt
     1081 agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg ggattagaaa
     1141 aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg tcgcagccag
     1201 aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa atcagaagcc
     1261 tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt ctacttggcc
     1321 acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg tacttactgc
     1381 tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa ccaaaaaaga
     1441 ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt gcaactaatt
     1501 gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc cagggaaatg
     1561 gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag ttgccttgcc
     1621 ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg tcaataaaaa
     1681 gcgcggtgct catcagctta aatgttaaaa accgcccgtt ctggtgaaag aactgaggcg
     1741 gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt tcagctcgtt
     1801 ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca atctgaatca
     1861 ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg atatcgttta
     1921 aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc ggtcggcaag
     1981 tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca aaacgactca
     2041 cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac agaaacgcca
     2101 gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc gcaagcaggc
     2161 tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc cacacaaacc
     2221 agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa cgattagcca
     2281 gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt tggccccagt
     2341 aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg ccgcttgcca
     2401 gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc tgaatagcct
     2461 agtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt aatcccccaa
     2521 accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt ttcgctgtta
     2581 ccccttcatt cagcgtcgca taccccactc ccgccagcag aataaattgc gcggtagtga
     2641 gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc aacgttacga
     2701 gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa acagcctgcc
     2761 aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc tgaacggcga
     2821 gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa cgcagagaac
     2881 gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac attgcaaagc
     2941 caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag gtaaacgcca
     3001 gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg tccaccgaaa
     3061 caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt ggaacaatca
     3121 gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt tcgttttttg
     3181 gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag tttaaagtga
     3241 ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc gttaaatgcg
     3301 catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc gcgtagcgaa
     3361 gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt tttacagagc
     3421 ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat ccaggctcac
     3481 tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga atctggctaa
     3541 actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg gggtggcttt
     3601 taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac agccagaaca
     3661 tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg tcaatctgtc
     3721 acgtttacct tacgagccca aacttaaata aaacttatac agagttacac tttcttacat
     3781 aacgcctgct aaattatgag tgttttctaa accgccccca taatttgcag tcattttgaa
     3841 aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc tcgtaaaacc
     3901 tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc caggcaacaa
     3961 tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc gcgacgtgcc
     4021 cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc aacgacctag
     4081 cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat tcgtggaatt
     4141 gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca tggtgcatgt
     4201 ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg tatggatatg
     4261 gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga cctgctggcg
     4321 tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct gatggcaaat
     4381 gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg cgccgatcgt
     4441 ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga tatcattcca
     4501 ttacgctagg ctttttcggc ctggatcatg ccatgttgcg cattatcgat acagaaacct
     4561 gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac ggaaaaatcg
     4621 tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa gcgatggcga
     4681 ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat gtgatcccac
     4741 actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc cgcgtactgc
     4801 ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg tggccagggc
     4861 tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag acaccgctgg
     4921 gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg cttatcgata
     4981 ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga cgtccgtcgt
     5041 tgatgacaac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac gttgccgaac
     5101 gctatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc ccggagctgc
     5161 gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagcg ggtaatgttc
     5221 cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac gatttaaagc
     5281 ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa agatggtcat
     5341 cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat tgcacctgag
     5401 aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg acgttgtcat
     5461 acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt ccccactctt
     5521 caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca acatcaacaa
     5581 acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt gcaacgccca
     5641 tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag ccatagccca
     5701 gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc ttcttcagaa
     5761 atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca tggacaatgg
     5821 cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg gcatcaatac
     5881 ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg cgaaaatgtt
     5941 tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact atccacaggt
     6001 gttcactgcg gtaattcgcc gcatcaaaac caggcacttc tgtttgtttt aatacacgac
     6061 gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg gaagaataac
     6121 cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc acataggccg
     6181 gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg cgcaaactgg
     6241 ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta aacccttcca
     6301 gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca cgcatacggg
     6361 tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa cgatgctggt
     6421 agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac ggctcggcat
     6481 cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc agatgaatga
     6541 cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg tctttttctt
     6601 cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag acctgataag
     6661 gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca tttgcccaga
     6721 caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt tccagattac
     6781 gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc ataatggtgt
     6841 tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct gcgcgcttgt
     6901 tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat tgacgctggt
     6961 agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca ttttttatgt
     7021 agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag atgcgatcct
     7081 gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt tgcaggtagt
     7141 ccaggacttt tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta tcgatgcgcg
     7201 tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc atgttattgt
     7261 tctttctggt tggaaacatc ttataagggt ggcaaaactc accggggatg caagcgaaac
     7321 agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg ttatgcagac
     7381 agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa tagtcaactt
     7441 caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt cgagatcgcg
     7501 ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg cattcaggta
     7561 actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat agatttccag
     7621 cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg gttgttcgat
     7681 tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca attgctggtc
     7741 gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa agaccagcgc
     7801 aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa actgcggttc
     7861 gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg tacccgctac
     7921 cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt tacgtgctga
     7981 tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctgacacta acagtttgcc
     8041 caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca gggattgcag
     8101 ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc ctgtagcata
     8161 gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata agcggggcgg
     8221 taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat caggtgataa
     8281 gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg ctgattgccc
     8341 gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg gacggacaag
     8401 gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta acgccgctga
     8461 tattccgcca ttcccgcttt gcagtccacg cagacctggt aatgaatatc ggcactttta
     8521 ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc ctgcaaactg
     8581 acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca gcgcgccacg
     8641 cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc cagaccttca
     8701 cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac tgatgcgcag
     8761 gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt ctccatagca
     8821 caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg
     8881 tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg taatttgccg
     8941 gttttcagaa tggtgctcag cacctctttt tgttctgcca gccatagcga aagggcttca
     9001 cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag cagatccgcc
     9061 gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga cattttctct
     9121 tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct ctcataataa
     9181 ctgagatttt atacagtata tttcttttcg gttgagaaat caacatcagc aataaagaca
     9241 cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt cgttatgacg
     9301 ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg ctccggtgaa
     9361 ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat
     9421 cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat taatatgctt
     9481 gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat cgtgccggag
     9541 atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact gaatgttgtc
     9601 ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg cctggcggca
     9661 gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag ccttttccgc
     9721 gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag ctcttccggc
     9781 aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga atacgctcag
     9841 caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa gtttgacttc
     9901 gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc accagtaggt
     9961 catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat gagcccgctt
    10021 gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg cggttatggt
    10081 ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga ggtctcccct
    10141 cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga gtttgccctg
    10201 catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg tcctgcagct
    10261 tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa tgtgcagaat
    10321 gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga tggcagccgt
    10381 catctggggg tggtactggc tacttcagag agtgttgttg acgccattga acgcgcgaag
    10441 cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc tacaaaaatg
    10501 cccgatccta gatcgggcat tttgactttt acagcttagc gccttctaca gcttcacgcg
    10561 ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga accagccagg
    10621 aaccaccgat gcacagcacg cttttcagtg ccaggtagtc acggtagtta gccggagaaa
    10681 taccacccgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc tgcagggctt
    10741 tcacgccgcc gttagcttca gccgggaaga atttgaactc tttcaaaccg tagtccatac
    10801 ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc ccttcggtag
    10861 cagctttcag cagcggctcg gtcagacccg ggctaattgc gaactgtgca cccgcttcag
    10921 tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc gcttcaggca
    10981 cttctttggc gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga gtcacttcca
    11041 gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt tccagttttt
    11101 ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca cttgttttcc
    11161 agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa aaagtgatac
    11221 aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat aattcacgtc
    11281 ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca gccagttccg
    11341 cttcgtctac cagcagtgtc agttcgcctg tctgtccatt cacacgaatg atgtccccgt
    11401 cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg atagctgacg
    11461 gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt ttgaaacacc
    11521 ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt ccgttcgctt
    11581 ttggcccctg atgacggaca acaacgacac aatcgcggtc cagcaaaccc gcttcaaagg
    11641 ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc acctggttct
    11701 caaccggcac ggcagaggtt ttcataaccg cacggcccag gttaccgctt aacacttttg
    11761 tcccaccatg atgagagaaa ggttgttcga aggaagcgat cacattgctg tcgagtgatt
    11821 tttccgcccc ttcccgccag tccagttcac cattattcag ccatggttca agggtataac
    11881 gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct gctttgagca
    11941 gttcacgcac cagaaccggt acgccacctg ccgcctggaa gtggttaata tcggccggac
    12001 cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag aagtcatccc
    12061 agttaatctg aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg tggttagtgg
    12121 aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca tcgatcatct
    12181 taccgatcgg catccattca ttaccattac cggtcatgcg tgtaacctga cgcgcagcgg
    12241 cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag cctggcaact
    12301 gcatccccat aaattccacc accatctggt tggtgttggc agtaccgtag aaagtacatg
    12361 ttcccggcgc atggtaagac gcggcttctg actccagtaa ggccatgcgg tccactttac
    12421 cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg cttgccatcg
    12481 gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc atcgtcagac
    12541 ccgggacaat cttgtcgcac acaccgagga acagagcacc atcaaacatg ttatgggaca
    12601 gccccaccgc cgcagacatc gctatcactt cgcggcttag cagcgacaat tccattccat
    12661 cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc tgaccaaccg
    12721 cattcgcttc atgcagggct ttacgaatga tttctggata gtgttcataa ggctggtgcg
    12781 cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac atgcttttca
    12841 aagaggcttt gttttctggc tggcaggcag cgaaaccgtg tgccaggtta ccgcatgcca
    12901 actgcgaacg atgaacggtc gacgttttcg cttgttctat ccgggcgaga taagcagagc
    12961 gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt ggattcataa
    13021 aggctcctga aattgagttg tcagagcagg atgattcaca acgcgtttca ttcagaggat
    13081 ttatgagtga aacgcctgta accggagctc atagggcaaa aacgtttcag tcagtgtaat
    13141 aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt gttctacaat
    13201 ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgctga tattactcaa
    13261 actcattcca ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg ggtccccagg
    13321 ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca gtaatggagt
    13381 ctacccattt ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc tgaataccac
    13441 gcatggtttc cagcagcaaa cgttcatagg catccgccag atgcgtctga ttaaaggttt
    13501 ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca aggccaggaa
    13561 ctttattcag tacctggata tccacgcctt catcaggttg cagacggata gtcagtttat
    13621 tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt ttgaaataga
    13681 ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag aatggcacac
    13741 cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct gtattgctgc
    13801 ttttgttcgc gccctcttct tccagatatc ccggcacttt tttgccctgg gcgaagcccg
    13861 cagtatattg cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg cggcgcaggg
    13921 acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca gatggcggag
    13981 acatcgcaat catgcagaga atttgtagca ggtggttctg aatcatgtca cgcatctgac
    14041 cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc acggtaatct
    14101 caacatgatc aatggtgcga ttgtcccagt tattcacaaa cagggagtta gcaaaacgca
    14161 gcgccaacag gttcagcacc gtttctttac caagatagtg gtcgatacgg taaacctggc
    14221 actcctcgaa gtattcgcca acctgatcat tgatttcctg cgaggtcgcc agcgacgtcc
    14281 ccagcggttt ctccatgact acgcgtgccg gtttagcatt cagttttgcc tcgccaagcc
    14341 ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaata gtgatacgat
    14401 ttttttgatc cagcatcgcg ccgagacggt tgaatgcagc agtgtcattg acatcgagat
    14461 tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg gtttctttca
    14521 tgaaagtttc gagcgcctcg cggacaactt tggtatatgc cgctttatcc cagtcagcac
    14581 gccctacgcc gataatccgg gtgtccgggt tgagctgacc gactttttcc agttgataca
    14641 gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg accaggtcac
    14701 aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta acccggtact
    14761 taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta cgagcttgcg
    14821 aaaactgtaa acacttatcc acccgtgcga ttacgggaaa agcgcgcaaa gcgcggcaaa
    14881 actgataaaa aaatcatcgt tttccattcg gtaaaaatct gacactgatc atgttatgaa
    14941 aaaaaataac aactttttta tctgcttttg tcattaacac ggcacacagg cgtaatatct
    15001 gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt gagtctcttt
    15061 acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc aaatcagagc
    15121 gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg agtattgctg
    15181 ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt cgcagcatgg
    15241 acacgcgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg aatggcactc
    15301 cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg gggaaaatat
    15361 ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa tctgccatca
    15421 accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga ttaggctctt
    15481 ctgccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt ccggtggtgt
    15541 actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac ggagacgtgg
    15601 tggtgctgat ttctcacaca ggaagaacaa aaaatctggt cgagctggcg cagctggcac
    15661 gcgaaaacga cgccatggtg attgccctca cctctgcggg taccccgctc gcccgggaag
    15721 caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg cccatggttt
    15781 ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact ttgcgacgcg
    15841 gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa tcgcgttttg
    15901 ataagcagtt acttaattta agtgacgatc gctaaaagcg actgtcactg tcctaatctt
    15961 atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat tcggatttca
    16021 tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccaa aaggcttcgc
    16081 agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa tcttgaaaaa
    16141 gttatcgcgg cgggtgccaa cgttgtacgt atgaactttt ctcacggctc gcctgaagat
    16201 cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg tcatgtggct
    16261 attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga aggcaaagtt
    16321 ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg tgaaggcgac
    16381 aaagaaaaag tcggtatcga ctacaaaggc ctgcctactg acgtcgtgcc tggtgacatc
    16441 ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg catgaaagtg
    16501 ttcaccgaag tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa caaacttggc
    16561 ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa gactgcggcg
    16621 ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga tctgaactat
    16681 gcccgtcgcc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa ggttgaacgt
    16741 gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc tgacgtggta
    16801 atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt cggcattcag
    16861 aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc gacccagatg
    16921 atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga cgtagcaaac
    16981 gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc tgggcagtat
    17041 ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa aatcccgagc
    17101 atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga agctattgcc
    17161 atgtcggcaa tgtacgcagc taaccacctg aagggcgtta cggcgatcat taccatgacc
    17221 gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctgcc aattttcgcc
    17281 atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt tacgccggtg
    17341 ccctttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa tctgctgcgc
    17401 gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccaaggcga cgtgatgagt
    17461 accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg ttgccggatg
    17521 cggcgaaaac gccacatccg gcctatagtt caatgatagt tcaacagatt tcgaatattc
    17581 tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg cttttttatt
    17641 tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg cgagttttca
    17701 gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc tcgacttctt
    17761 cattcatccg ccgctcaatc gtatgatcat ccgcctctaa cagatcatcc atcggtgggc
    17821 gcacctgaat cgtcagacga tgcgtcttgc catcataaat cggaaacagc ggtacaacgc
    17881 gcgcacggca tactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta taggtggcaa
    17941 agaaatccac aaattcgctg tgctctgggc catgatcctg atcgggtaaa taatatcccc
    18001 agtacccctg acgtaccgac tggatgaatg gtttaatacc gtcatttctc gcatgcagac
    18061 gaccgccaaa gcgacgacgc accgtgttcc agacataatc aaaaaccggg ttgccctgat
    18121 tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca ggaatatcga
    18181 cggcccaacc gtgcggcacc agaaagataa ctttctcgtt attacgccgc atctcttcga
    18241 tgatctccag cccttgccag ccaacgcgcg gctgaatttt ctccggcccg cgtattgcca
    18301 actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca acaatcgctt
    18361 cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac gcacgacggc
    18421 gtgagctttt tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg ggatcacgga
    18481 actttggcgg cgttaaagcg atacccgcca tcgctgctac acccagccat gctccccagt
    18541 agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta ttttttttcg
    18601 tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag tgtagcggca
    18661 caacttgccc cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc tgcgaatgga
    18721 tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca ggaattcgcg
    18781 acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg ggtttacggc
    18841 ctgctggttt atccatactt catagtgcag atgcggccca gttgaacgtc cggtattacc
    18901 ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca gaatcttgcg
    18961 caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat aataacctgc
    19021 tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca ctggcgtacc
    19081 ttgtggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgacctgtca ccggattagt
    19141 tcgacgcggg ttaaagttag acgagatacg gaactgtttc gccgtcggga atcgcaagaa
    19201 tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcctcag cgcggattgc
    19261 gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt gctcacgttt
    19321 accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt tcagtttgcg
    19381 gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc tggttaaacc
    19441 ggcgtttctg gcgctggcaa caaagctccc tccgacggta cctttcagca gattgttaac
    19501 ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag tacggtcata
    19561 ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc cgtccgcggt
    19621 taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt cggcagcagc
    19681 aagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc ttagcgtatc
    19741 gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt catccagttc
    19801 gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac tggcttcagg
    19861 taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg gcgtggcatc
    19921 acggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg tgagcgaccc
    19981 caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag agcgggctat
    20041 ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat actggttggc
    20101 taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg tccccagggg
    20161 atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga ccgctggcct
    20221 gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg ttcttatttc
    20281 atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac caagcggtgt
    20341 cagtccgaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat aacctttccc
    20401 cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggccaatt gtgcctcaaa
    20461 atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca ctaattttcc
    20521 atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat gcatgttgaa
    20581 atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat catcatcgcc
    20641 gtgaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg ttacctgctt
    20701 cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg ggccaaccca
    20761 aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg aataatcatg
    20821 ttctgaagcg ccgtcaggca gtaaaacctc cgtttctgtt acaccatcag caatggcaga
    20881 agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg cctgtgttgc
    20941 acctccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat gtaacataat
    21001 gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca tgactactgc
    21061 aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt ttcttttggc
    21121 caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat tttgacttta
    21181 cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg gctggtaaca
    21241 cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt accgcagaag
    21301 ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt acgccctggt
    21361 acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca cctgattaac
    21421 gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactattagc gcgagcattg
    21481 ttaaattgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga tgtaaatggc
    21541 caagtggcgt tatatgacct tattgaccag ttgcgtcgtg aactggattg tggcgtttta
    21601 atggtttctc acgatctgca tctggtgatg gcaaaaaccg atgaagtgct gtgcctgaat
    21661 caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga gtttatttca
    21721 atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca taatcatcgt
    21781 cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg attgaattat
    21841 tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg ctgggttcgt
    21901 ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc tcattacttg
    21961 gtgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt gccgttacgc
    22021 tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg atcgacacgt
    22081 tattagggat tatggcgcac agtgccctgt cgctgggcct agtggtcgtt agtctgatgt
    22141 ctaacattcg tgttgatttg atggcttacc tgttcggtga tttactggca gtgacgccag
    22201 aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg ttctggcaat
    22261 ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat ggtgtgaaat
    22321 tacagcaagt gaaattgttg ttgatgctgg tgacggcatt gacgattggt gtagcgatga
    22381 aatttgtcgg tgcgttgatt attacttcgt tgctgattat tcctgcagct actgcgcgtc
    22441 gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg atggtggcag
    22501 taactggcgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg tcggtggtgc
    22561 tatgtgcggc actgttattt attatcagta tgatgaaaaa gcaggccagc taatctatcg
    22621 ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt cgctatctct
    22681 ggctggcctg ataagacgcg aacagtgtcg catcagacac actgccagtg tcggatgcgg
    22741 cgcgagcgac caatccgact tacggcattt ctggcggcgt tatgccaaag tgattccacg
    22801 cccgcgtcgt cgccatacgc ccgcgcggtg tacgctgcaa aaagccttgc tgaatcaaat
    22861 aaggttccag tacatcttca atggtttcac gttcttcgcc aatggctgcc gccaggttat
    22921 ccagacctac cggcccacca aagaacttat cgattaccgc cagcaacaat ttgcggtcca
    22981 tataatcgaa accttcagca tcgacattca acatatccag cgcctgagca gcgatatctg
    23041 ccgagatggt gccatcgtgc ttcacttcgg cgaaatcacg cactcgacgc agcagacggt
    23101 tggcaatacg tggcgtaccg cgagcgcgac gagctacttc ctgcgcaccg tcatcactca
    23161 tctcaagccc cataaagcgt gcactgcgac tgacgatata ttgcagatcc ggcacctgat
    23221 aaaactccag acgttgcaca ataccaaaac ggtcgcgcaa cggtgatgtc agcgaacctg
    23281 cgcgcgtggt tgcgccaatc agggtaaacg gcggcaaatc aattttaatg gagcgtgccg
    23341 ccggaccttc accaatcatg atatccagtt ggtagtcttc cattgccggg tacagcactt
    23401 cttcaacaac tggcgataaa cggtggatct catcaataaa cagcacgtca tgcggttcaa
    23461 ggttagtgag catcgcagcc aaatcgcccg ccttttccag caccggacca gaagtcgtgc
    23521 gtaaattaac gcccatttca ttggcgacaa tgttggcaag cgtagtttta cccaaccccg
    23581 gaggaccaaa aatcaacaaa tgatcgaggg catcgccgcg cagtttcgct gctttgatga
    23641 aaatctccat ctgtgaacga acctgcggct gaccaacata ctcttccagt aatttagggc
    23701 gaatggcgcg atctgctaca tcttccggca aagtggtacc ggcagaaatc agacggtctg
    23761 cttcaatcat cctttacctc ataacgcggc gcgtagggct tcgcgaatta atgtttcact
    23821 gctggtgtca gggcgagcga ttttgctcac catgcggctt gcttcttgtg gtttatagcc
    23881 tagcgccacc agcgcggcaa ctgcttcttg ttcagcatcg tcggtcgccg ggctagcagg
    23941 agacgtgagt accaggtcgg cggctggcgt aaagagatcg ccatgcaaac ctttaaatcg
    24001 gtctttcatt tcaacaatca agcgttcggc ggtttttttg ccaatacccg gcagtttcac
    24061 cagtgccccc acttcttcac gctcaacggc attaacgaac tgctgcgctg acattccgga
    24121 gaggatcgcc agggccaact tcgggccgac gccgttggtt ttgatcaact ctttgaacaa
    24181 cgtgcgttct tgtttattgt taaaaccgta cagcagttgc gcgtcttcac gcaccacaaa
    24241 gtgggtgaaa acgatcgctt cctgacccgc ttcagggagt tcataaaaac aggtcatcgg
    24301 catatgcact tcatagccta cgccgcccac ttcaattaac accagcgggg gttgtttttc
    24361 aatgatgatg cctctgagtc tgcctatcac atgacgctcc tgcgtaatga atcaaagata
    24421 atgctgtatg ataaaaaaat gctggataga tatccagcga aggatgaaga aaacttgcga
    24481 ggtgtcccga tgatctgaaa aatggcacag tataatttat tcaacaggtt atattggaag
    24541 caaatatttg aatattacat attcagtgaa gaaatgcgta ataaaaatat acattgcgac
    24601 tcctgaaaaa taattttttt atgctattac gtatatttgt atctatttca atggaatgac
    24661 aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt tacatgtata
    24721 ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa tcacgtcggg
    24781 atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt tcccctttca
    24841 accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta tgctataaag
    24901 cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca ggttccctcc
    24961 cggctacgca aactttacca cacgggtttt aaatacgaat cttcgcgcca gttctgttca
    25021 aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga aatagagacg
    25081 tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa attctggttc
    25141 ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg gcatcatccg
    25201 ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agccggaact gaccgaggcg
    25261 gtataactta acgcagtcgc cctctcgtca ggttcagccg cgattcgctc atctgcatcg
    25321 cattctgact aacgtggcag tgggtgatgg caatcgccag cgcatcggcg gcatccgcct
    25381 gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg cttttttcgg
    25441 cactacccat acctaccacc gtttgcttta cctgacgtgc cgcgtattca aataccggca
    25501 attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt ttcagggctg
    25561 agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc tggaactggg
    25621 tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa tcatccactt
    25681 tggtgcgtat gcatccgcta cccaggtagg acagttgcct acctacctgg cggatgacgc
    25741 cgtagccggt cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc acgcgtctcc
    25801 gttttgctgt ttagcaggca tcatcagaga gtcgctgcaa cctcatcaga gatttcaccg
    25861 ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag acgcatcagt
    25921 ttcggcgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga aacttccgcg
    25981 ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat ttcttcccat
    26041 gcagtgtaga catcaatcgc gccgtcatca tatgtcacaa cgtcttcagc acctgcttcc
    26101 agagctgctt ccatgatggt gtcttcgtcg cctttctcga aggagatcac gccttttttg
    26161 ctgaacagat aagctacgga accgtcagtg ccgaggttac cgccacattt gctgaatgcg
    26221 tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat catgattgcc
    26281 gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc atcatcaccg
    26341 cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt agacagtgct
    26401 ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc cagcttagcc
    26461 gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc atcctgcgca
    26521 gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat atctccagat
    26581 agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc ggttgctcca
    26641 ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca gatgttcagt
    26701 gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg tattacgcgt
    26761 cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta ccgtgcgctg
    26821 acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtaacctctt cctttacttc
    26881 gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta ccgactgcca
    26941 gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt gtgcatagat
    27001 gaccactaag atcgaaacgg gacgcttata cactttatcc ttcacgctgc ctcttcgttg
    27061 actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc actcagttgc
    27121 cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat tctcagcctt
    27181 cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc tcggtgcttc
    27241 agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac ggatattgtc
    27301 ggtgccagtc agcagcatgg tcagacggtc aagaccgaat gccagacctg cgtgcggcgg
    27361 agtaccgtat ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt cctcttcgtt
    27421 gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac gtactgaacc
    27481 accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg cattttccgg
    27541 tgcagctttc agttctgcag cagtcatatc tttcggtgag gtgaacggat ggtgcattgc
    27601 cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc acagcggtgc
    27661 ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggagca gtgcgcccat
    27721 cccgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc catcttgcgc
    27781 ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg ctaccgggct
    27841 gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca gacctttcgc
    27901 gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca gcgatgcgcc
    27961 acccggaaca cgtagagcgg caacacggcc tttcggatcg ttcgctggcc ccgcaaatac
    28021 tgcaaactca acagatttca gcagatcggc aacgtcggtc agttccatcg ggttacgcag
    28081 atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta ccgggaaatc
    28141 gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt ccatcacttc
    28201 acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa attcaggctg
    28261 acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt aacggtcaaa
    28321 accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg cgtagaattt
    28381 acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg tggctttggt
    28441 cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc gcaccaggct
    28501 ggtgatttta gcgcgggttt tcaggcgttg cgccatttcc gggcgacgca ggtcgaggta
    28561 gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg gcagaacatc
    28621 tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg tcgccatatc
    28681 gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa tgcagaactc
    28741 attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga aaaatacctg
    28801 cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat cacgacgacg
    28861 gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga gctgtccaca
    28921 atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc cctgctgcgc
    28981 aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag gcggctatta
    29041 tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct cgttaccact
    29101 ttatatcggg cctgaaatca gactctacgc cagtttgcta taaaggtgtt gcccgaactc
    29161 ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg tgtgaggcac
    29221 gattttgttt tttcaggagt cagcatgctt gaacttaatg ctaaaaccac tgcgttggtg
    29281 gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac tgccgatgag
    29341 gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca gcccgtgttt
    29401 ctggtgcgag ttggctggtc tgccgattac gccgaagcat taaaaca
//
LOCUS       DAJTPG010000044        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTPG010000044 DAJTPG010000000
VERSION     DAJTPG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO230_002036"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO230_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5691800.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO230_002037"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO230_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5691801.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO230_002038"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO230_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5691802.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO230_002039"
     CDS             4843..5082
                     /locus_tag="OO230_002039"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691803.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO230_002040"
     CDS             5092..5262
                     /locus_tag="OO230_002040"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5691804.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO230_002041"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO230_002041"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5691805.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO230_002042"
     CDS             6724..6990
                     /locus_tag="OO230_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5691806.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO230_002043"
     CDS             7084..7773
                     /locus_tag="OO230_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5691807.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO230_002044"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO230_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5691808.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO230_002045"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO230_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5691809.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO230_002046"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO230_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5691810.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO230_002047"
     CDS             complement(10475..12193)
                     /locus_tag="OO230_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5691811.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO230_002048"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO230_002048"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5691812.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO230_002049"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO230_002049"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5691813.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO230_002050"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO230_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5691814.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO230_002051"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO230_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5691815.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO230_002052"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO230_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO230_002053"
     CDS             18432..18746
                     /locus_tag="OO230_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5691816.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO230_002054"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO230_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5691817.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO230_002055"
     CDS             20570..21862
                     /locus_tag="OO230_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5691818.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO230_002056"
     CDS             complement(22016..23665)
                     /locus_tag="OO230_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5691819.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO230_002057"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO230_002057"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5691820.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO230_002058"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO230_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5691821.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO230_002059"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO230_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5691822.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO230_002060"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO230_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO230_002061"
     CDS             28753..29034
                     /locus_tag="OO230_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5691823.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7283 a         7775 c         7353 g         6632 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcgctgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacactaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTPG010000045        28541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTPG010000045 DAJTPG010000000
VERSION     DAJTPG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..644
                     /locus_tag="OO230_002062"
                     /pseudo
     CDS             1..644
                     /locus_tag="OO230_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            641..1027
                     /locus_tag="OO230_002063"
     CDS             641..1027
                     /locus_tag="OO230_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691824.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     EIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1052..3265
                     /gene="tssI"
                     /locus_tag="OO230_002064"
     CDS             1052..3265
                     /gene="tssI"
                     /locus_tag="OO230_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5691825.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3288..3733
                     /locus_tag="OO230_002065"
                     /pseudo
     CDS             3288..3733
                     /locus_tag="OO230_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3755..4162
                     /locus_tag="OO230_002066"
     CDS             3755..4162
                     /locus_tag="OO230_002066"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5691826.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4159..6447
                     /locus_tag="OO230_002067"
     CDS             4159..6447
                     /locus_tag="OO230_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5691827.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6444..7433
                     /locus_tag="OO230_002068"
     CDS             6444..7433
                     /locus_tag="OO230_002068"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691828.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7533..7736
                     /locus_tag="OO230_002069"
     CDS             7533..7736
                     /locus_tag="OO230_002069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691829.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7754..8074
                     /locus_tag="OO230_002070"
     CDS             7754..8074
                     /locus_tag="OO230_002070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691830.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8140..8372)
                     /locus_tag="OO230_002071"
                     /pseudo
     CDS             complement(8140..8372)
                     /locus_tag="OO230_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8482..9252)
                     /gene="yafV"
                     /locus_tag="OO230_002072"
     CDS             complement(8482..9252)
                     /gene="yafV"
                     /locus_tag="OO230_002072"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5691831.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9433..9879
                     /gene="ivy"
                     /locus_tag="OO230_002073"
     CDS             9433..9879
                     /gene="ivy"
                     /locus_tag="OO230_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5691832.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9922..12366)
                     /gene="fadE"
                     /locus_tag="OO230_002074"
     CDS             complement(9922..12366)
                     /gene="fadE"
                     /locus_tag="OO230_002074"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5691833.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12606..13184
                     /gene="lpcA"
                     /locus_tag="OO230_002075"
     CDS             12606..13184
                     /gene="lpcA"
                     /locus_tag="OO230_002075"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5691834.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13390..14157
                     /locus_tag="OO230_002076"
     CDS             13390..14157
                     /locus_tag="OO230_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5691835.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14128..14868)
                     /locus_tag="OO230_002077"
     CDS             complement(14128..14868)
                     /locus_tag="OO230_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5691836.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15252..15773
                     /locus_tag="OO230_002078"
                     /pseudo
     CDS             15252..15773
                     /locus_tag="OO230_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15949..16446
                     /gene="rayT"
                     /locus_tag="OO230_002079"
     CDS             15949..16446
                     /gene="rayT"
                     /locus_tag="OO230_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5691837.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16663..18375)
                     /locus_tag="OO230_002080"
                     /pseudo
     CDS             complement(16663..18375)
                     /locus_tag="OO230_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18347..19132
                     /gene="lafU"
                     /locus_tag="OO230_002081"
     CDS             18347..19132
                     /gene="lafU"
                     /locus_tag="OO230_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5691838.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19203..20258
                     /gene="dinB"
                     /locus_tag="OO230_002082"
     CDS             19203..20258
                     /gene="dinB"
                     /locus_tag="OO230_002082"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5691839.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20310..20603
                     /gene="yafN"
                     /locus_tag="OO230_002083"
     CDS             20310..20603
                     /gene="yafN"
                     /locus_tag="OO230_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5691840.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20606..21004
                     /gene="yafO"
                     /locus_tag="OO230_002084"
     CDS             20606..21004
                     /gene="yafO"
                     /locus_tag="OO230_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5691841.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21014..21466
                     /locus_tag="OO230_002085"
     CDS             21014..21466
                     /locus_tag="OO230_002085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5691842.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21712..22755
                     /locus_tag="OO230_002086"
     CDS             21712..22755
                     /locus_tag="OO230_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5691843.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22817..23431
                     /locus_tag="OO230_002087"
                     /pseudo
     CDS             22817..23431
                     /locus_tag="OO230_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23488..24945)
                     /gene="pepD"
                     /locus_tag="OO230_002088"
     CDS             complement(23488..24945)
                     /gene="pepD"
                     /locus_tag="OO230_002088"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5691844.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25207..25665
                     /gene="gpt"
                     /locus_tag="OO230_002089"
     CDS             25207..25665
                     /gene="gpt"
                     /locus_tag="OO230_002089"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5691845.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25757..27001
                     /gene="frsA"
                     /locus_tag="OO230_002090"
     CDS             25757..27001
                     /gene="frsA"
                     /locus_tag="OO230_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5691846.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27059..27460
                     /gene="crl"
                     /locus_tag="OO230_002091"
     CDS             27059..27460
                     /gene="crl"
                     /locus_tag="OO230_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5691847.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27499..28539)
                     /gene="phoE"
                     /locus_tag="OO230_002092"
     CDS             complement(27499..28539)
                     /gene="phoE"
                     /locus_tag="OO230_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5691848.1"
                     /translation="MALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYMSDNAS
                     KDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDL
                     GSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDG
                     LNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRG
                     TGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLR
                     PSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINN
                     DDTVAVGMTYQF"
BASE COUNT         7041 a         7102 c         7584 g         6814 t
ORIGIN      
        1 atattgatgg caatatcatt atacataagg atggtaggag gacgtattta ttaaatcaaa
       61 atactggttc gattacggct gtgataagat ggaagtatga gtagtataag gataataccg
      121 ttacagatga ctggactata tgggaaaaag ctatttttat gcgagcgata tatttatctg
      181 ttcagcaggc atggaatggg aaaataactt attctgtttc cggtgaatct gagttcgcta
      241 aaaaatttca gggtaaagct ttgccttttg atgtaagaat aatttctgca tctcagaatg
      301 aagactggtt ggttattgca acaaaggttt tgcctggtgc tgatttaaga acatatgttg
      361 attttaaaaa cagtacggtt catgttgatt cagcgggcct ggaaaaagta gcgaaatgta
      421 ttaactgtaa taatacgttg caagtaaata tacctcatga agccggacat gtacttggtt
      481 acttagatga tgattatgat tcttcgtctc cgtatgttgg agatattagt gggttgatga
      541 atgtgggaat ggagctttgg gaacgatacc ttaaaaacgc aactataacg ttgaatatca
      601 taatgcctga aactaaattt actttattga atgtaaccaa atgaaatttt tctgtattat
      661 agtgattttc ctttcaataa attacatagc tatagtgaat gttttcaagt cttctgcagt
      721 tgctcaatcc accaaatatt atgaagaaaa tgatatacat gatggaagta atgaaatctt
      781 ttttatgaag aaatatgggc cgcctgctca tgagttcgtg aggccacgat ataaatttgt
      841 tggtgagtat tatatcggct tgaatcaaac ttatcgcata acagatcctg aatttcaaga
      901 tgttcctatt aaggaaatgt tctggaattt gcagggggat ttgaatctga catgttggtt
      961 tcattataaa gacgggcaat ggagagtgat tacttatatt ttttggccac caggggctag
     1021 attttaatcc ggtattaaag gagcaaatat catgagtttt gtatctacgg gtaataaacc
     1081 agttggaaac ggtggacccg tcatcaccac accaccaata gctggcgaaa gtggcggtat
     1141 gagcacaggt tcagctgtaa ccgatgtatc tggtgccgcc gaggagatgg cagaacaggc
     1201 tgcggcggac ctgtttggtg cgctgccgga accgtcaggt ctggttaaag ccgcagtagc
     1261 tgctgcgcag gctgccgctg cagcagcagg gatctcggat atggctggtg cagttcagga
     1321 tgcagcggcg tcgcttgcag ccggtgctcc gggggcgcat aacgtcacag taagtggtag
     1381 tgcggtgccg ccgcagatgc ttttgtttgc aggcatgaat ggttcggaag ggcttgggaa
     1441 tctgttttct tacacagtac agcttaaaac gctggacgcc ctgaacctgg ggtacgtttc
     1501 ccccgctgct aacctgccgc ttcagctcat ggtgggcaaa gacctgtgcg tcagtatcga
     1561 actggacggc ggcggaaaac ggtatatcag cgggctggtg acggcggcga gggtggtcgg
     1621 ccatgagagc cgttcggtaa cgtatgaatt acgcattgag ccatggctga agctgctgac
     1681 ccataccagc gactacaaag cgttccagaa caaaaccgtg gtggatattc tcgatgaagt
     1741 actggatgaa tattcctttc cggtggaaaa gcggctggtg gaaaattatc caccccgcgc
     1801 ctggcaggtg cagtatggtg aaaccgactt cgatttcatc cagcgcctga tgcaggaatg
     1861 ggggatttac tggtggtttg agcacagcga gaacagccac acgctggtgc tggtggatgc
     1921 catcaatggt cataaagcgt gccctgactc gccgctggtg gagtggcacc aggaagggct
     1981 gaaactggat aaggaattta tccacaccat tacggccagt gagaggctgc gtactggaaa
     2041 gtgggtgatg gacgattttg attttatgaa gcctcgttcg ctgcttaaaa gtaccgtggc
     2101 cagtccgcgt gataccggtc atgctgaata cgagcattac gaatggcctg gtgattactt
     2161 cactacgggc gaaggcgaga tgctgacgcg cattcgtatg gaagcgcagc gcagcccagg
     2221 cagccgggca catggggccg gtcatatccg taccctcatg accgggtaca cctttacgtt
     2281 gatgaatcac ccgacggcag aaattaatca ggaatatctg ctggtacaga ccactctgtt
     2341 tttacgggat aatgcgcagc acagcggtca ggatcagcat tttacctatg tcaccacgtt
     2401 tgaactgcac cccaccagcg aagtttaccg cccacaacga accctgagca aaccccacac
     2461 caaaggcccg cagagcgcca tcgtcaccgg cccggcgggg caggaaatct ggacggataa
     2521 atatggccgg gtgaaggtgc agtttggctg ggaccgttac ggaaagaatg acgaaaacag
     2581 ttcctgctgg atccgcgtca gctatccgtg ggcgggtaaa ggattcggca tgatccagat
     2641 cccgcgtatt ggccaggaag tactggtgga tttcaaaaac ggcgatccgg atttgcccat
     2701 catcgtgggg cgcacctata accaggacac catgccgccg tgggggctgc cgggcatggc
     2761 gtcgcagagt gggatattca gccactcatt gcagggaggg ccgactaacg gaaatatgct
     2821 gcgcttcgat gataaaacgg gtgcggaaga ggtgaagttc catgcggaaa aagacctcaa
     2881 taccacggta aaaaacaatg aaacccacac ggtcatggtc gatcgcataa aaaccattgt
     2941 caaaaatgaa accatcagcg tgggagaaga ccgtaatgcc accatcacaa aaaatgatgg
     3001 tctttccgtc aaactggctc aaaccatcaa tgtcggaacg acatatcgtc tggatgttgg
     3061 cgatcaattt acccttcgtt gcggcaatgc agcgcttgta ttacacaagg atggctcgat
     3121 tgagttttgc ggaaaacaac tgttactgca caccagcgat gttatgcaac tcattggcag
     3181 tggcattgat atgaatccgg atggcggcac agccatgact gcggatgata ttgccccgtt
     3241 acctaccccc gacgtttctg aataaaaaat ataaaaggaa tttttctatg accagccttt
     3301 accgtattca ggagggctgc tttgccctgc ctgaaacttt tcttgatcgc accgtcaata
     3361 tcttcgtccc atcgggcaat gaaagggcaa ctcccagtct gaatattttc cgcgatacgc
     3421 tcaggccaga tgaaaatctg accacctata ttgaccgcca gatagccttg atgaaaaaaa
     3481 aaacctaggc cagcaccggg tactttcgcg gggaacggca ctggcgggaa ctggcgaagc
     3541 tgccatgaca ggtgaacaga ttctcgcaac tcatcgttct ggaaaaacag aagtgtacca
     3601 gcgtcaggcc gggtttatca ccgggccagc aaaggtacta atgttgacgc tgaccactca
     3661 gcgtcctttt gatgatcaca ccgaccagct atggactgca tggttgacta gttttcagcc
     3721 tgctaaaagc taaaaaaaac agggaggttt tgtcatgtat gaagccgcac gggtggatga
     3781 ccccatctac cacaccagcg cccttgccgg gtttttgata ggggccatca taggtatcgc
     3841 cattattgcc gtcgccagct ttgctttttt tacctgcggc tttttggccg ggctgatttt
     3901 gagttttctg gctgatcaga tagcttccgg agtcctgcaa ctgggtgagg ctattgggcg
     3961 ctcgatacat tccacagtgg gaaaaattct gaccggttct gataatgtca gcaccaacag
     4021 tcgcccggcc gcccgggccg tactgagtac ggtgatatgc gaagaccatt ccccggaaat
     4081 ccgcattgcc caaggttccg gcaacatcta catcaacagc cagctgcccg taaggatgac
     4141 cacactgagt gtgatgcagt gattgaggac ggctccccca atgtctttct cggcggcggt
     4201 acgcagacgg tactggaaat cagcccggaa atccctgact ggctgcgtca ggtggtggat
     4261 gtgctgtatg tggtcgccgg aatgcttggc gggctggctg gcgcctggcg tcaggcggca
     4321 aagatggggt cgaaatttgg tacaaaatgt gcggcaaaat ttattggtgg cgaaatggtc
     4381 gggatggcca tctctgacac agtcatggga ttgttcagca atccggtaga tgtcaccact
     4441 gggcaaaaaa ttttgctgcc ggagacggat tttaccctgc caggacgcct gcctgtcacc
     4501 tgttcgcgct tttacgccag ccacctggaa actgaaggat tgctgggcag aggatggcgg
     4561 ctgaactggg agataaacct gcgtgaggat gaaacatata tcaccttcat cggtgtacag
     4621 ggtcgggaac tgagttatcc gaaagagatg ctgataccgg gccaccagat attcgacccg
     4681 gaagagcagt tttacctgag ccgtctgcat gacggatgct atgtgctgca ttacaccgac
     4741 tgcagctatt acgtgtttga tgaatttgat gaccacggcg tcgcgccgtt actttttatg
     4801 gagacgccgt accggcagcg tattgctttc gggcgtgaga acggcaggct ggtgcgggtg
     4861 gcctccagca gcggacacca tctgttattg caccgcacga tgacgcaagc aggggaacgc
     4921 ctgtcgcata ttgaactttt gaagggcggg cgtcccggta acctggtgga atatcgttac
     4981 gacgataacg ggcaactgac tggcgtggtg aaccgggctg gtgtgacggt gcgccagttt
     5041 gcctatgaaa atggcctgat gacagagcat cgcaacgcga cgggctttac ctgtacctac
     5101 cactgggaag agattgaagg tttcccgcgc gtggtggaac acacgaccag cgacggcgag
     5161 gattaccggt tccactatga ttttgctggc gggcagacgg tggtaaccgg caggccggag
     5221 cagaagtggc agtggtggtt tgatgaagag acgtatgtga ctgcgcaccg gacgccgggc
     5281 ggcggaatgt accgcttcac gtacaacgaa aaccattttc cggtagcggt ggaactcccc
     5341 ggcgaacgca gggtaacgct ggaatacgac accctgagcc gggtggtgaa agagacggac
     5401 ccggcaggcc gggtgacaca gacgcagtgg aacggcagtt ttgctgaaat cacgcgccgg
     5461 gcgctggatg acgaccacgt atggaaggcg gattacaacg aacacggcca ggtgatacgg
     5521 gagacggacc cggaagggcg cgtcacgcgg tacgggtatg atgatcaggg gctgccggag
     5581 acggtctgcc accccggtaa acagcaggac cgttacacct ggaacgcgct ggggttactg
     5641 agcagccacc gtcgcatcac gggcagtgtg cagtcctggc agtatacgca gcgcgggatg
     5701 ctggcgcgcc atacggatga agagaaacgg gagacgcgct ggcagtacac cccggaaggg
     5761 ctggttgcca gtctgagcaa cggtaacggg gctcagtacc ggttcagcta cgacggcgac
     5821 ggacggctga cgggagaaca gcgcccggac ggcctgataa ggatgtttgc gctgaatgcg
     5881 gacgggtttc cggtcatcat cccgacgcag ggcacagagg gcggggtgcg caatgaacag
     5941 caggagcgtg atgcgctggg ccgcctgctg cgcagtgaca cgcagcacag tacacggacc
     6001 ttcagctaca accgtcttga ccagataacg gaagtgacgc tcacccccac ggaagaaggg
     6061 gaacggctgc accacatgca ggcggacacg gtgcgttttg cgtatgaccg cagcgggtgg
     6121 ctgacggcag agcacagcgt gcacggcagt atcaaatacc ggcgcgatgc gctgggcaat
     6181 cccaccgata tcacgttgcc ggacgggcag catctcagtc atctttacta cggcagcggg
     6241 catctgctcc agacggcact ggacggtatc acggtcagtg aatatgagcg tgacagcctg
     6301 caccgtcagg tgatacgcac gcaggggaaa ctggcgacgt tcagcggcta caacgccgac
     6361 aaccggttaa gctggcagcg cagcctgcca ggtggcagcc agaatccaca gcaggcggta
     6421 ctcccccggc gcagaacgac agcgtgacgg cgcgggacta tatctggaac gcagacggtg
     6481 aagtcggcgg catcaatgac aaactgcgcg gttgcctggt gttcagctat gaccgcagcg
     6541 gctggctgac cagccgcacg gggcagatgt atgaccatga ccattattat tacgacaaag
     6601 cgggcaacct gctgacggat gagtatcagg gagcggtgat ggataaccgc ctgccgggtt
     6661 acgggcgtga ccgctaccgc tataacgaat ggggcgaact gacagagcgc agggaccagc
     6721 agctggagtg gaacgcgcag ggccagttaa cgcgggtcat cagcagcaac agcgagacgc
     6781 ggtatcagta cgatgcgctg ggcaggcgca tcagcaaggc gaccagcaat ctccataccg
     6841 accggggaga acgaagccgg acaacgtttg tgtgggaagg gttccggctg ttacaggaga
     6901 caacgtggca gggaaagcgg acatacctgt acgatgcgga acagccgtac acgccggtgg
     6961 ctgccatcac ggggcgcgga gagagccaga aaatctggta ttaccacacg gacctgacgg
     7021 gcacggtaca cgaggtaacc gcgccggacg ggacgctggt gtgggcgggc taccaggcgg
     7081 gatttggtga aaaccggggc gacatcagca acagcggggc gtactttgag cagccgcagc
     7141 ggctgccggg gcagtacttt gacgaagaaa cagggctgca ttacaatctg ttcaggtatt
     7201 atgcgccgga gtgcgggagg ttcgtaagcc aggacccgat aggattaaac ggtgggttga
     7261 acttatatgc ttatgcgcct aatccgttag gttggatcga tccgctagga ctgaattctt
     7321 taggaataga ttcaaataat atatttagag gagatggtaa ctataaaggt ggcggaattg
     7381 gacagccaaa gagtggcaca atatcagcac aggacattat tgatcatctc taagaaaaag
     7441 gtgatgggcg tttaacatct tttagtacta aaaaaacatc tagttccaac tctcgtggtg
     7501 cagatttttt ctcaaaaaac catgtcttca aagtggcagt ttctgatctc gatgatttag
     7561 ttaaaaataa tcagatcact gttttaacac aagaaaaagt aaaagcaata tttggtttag
     7621 aatcaagcaa gaaaattaga aaagaacttg gtaatattct tcagaatatg gctaggaata
     7681 atgaggtgtt agttcatggg caaattcttg gaaataaagt tagccgatgt aattagttag
     7741 tggagaggaa gttgtggacg aaattagaat aagaattgct ggtaatacgg ctgcgccttg
     7801 tttccatgtg ctaaaaaata agggatataa agtaacaatg gaaactcatg cattatctga
     7861 cgattgttta gattgtgtat atgattggta tgcggaaaaa aatggacaat tttttagtgc
     7921 aacgagtcca gaagagttac ttggattagt tgcaatgtgg gaagttcgtg gtaatgactg
     7981 gagagctaat ttgaatgaga taaatactta tcaaaaagcc atagaagaat ccccgttttt
     8041 tgataaaaac ggcaatgaaa tagagtctga ctaaatttta ttgatatagc aacgataata
     8101 accggaaatt tcttccagat ctttccggct ttttattatt taactttcgt tcagcacact
     8161 aaacatcccg tctcgcatcc ccttaactgg ctgtttaacc tgataaagtt ccctgcgcag
     8221 ttcctgtact tcgttgttat cccaagcagg atcaaacatc cctccgagat cttcacggta
     8281 acgcccgtgc cgatctcaaa tcccgctttt ctcagccatt tggcgctgat tttgatcacc
     8341 ggcgtgtcgc tgcggttggg cagataaccc acggtacagc gacgctgtgt tttggaaatg
     8401 gtgacttctg gcgtaatatt gtgtttaagt taggggcttt cgtaatctag cccagccccc
     8461 ggccctaaac acaaccccca ctcaccacaa cctaaactca tccgcatccc gccatgccgg
     8521 aaacttttct ctatattccc gcaatgccac catcgacagc tccgcatcaa tacgcgttgc
     8581 cttatgcgcg tcggcggtag cgataatctc gccttgcgga ttaatcaccc ggctgtcacc
     8641 gcgataatgg cagccgttgc catcgctgcc gacgcgattg catcccgcca catacgcctg
     8701 attctcaatc gcgcgggccg tcagcaatgc ctgccagtgc agagagcgcg gagctggcca
     8761 gttggcgacg tacagggcga ggtcataatc gttgagattg cgcgaccaca ctggaaaacg
     8821 taagtcgtag cacaccagcg gcaaaatacg ccagccgcgc cattccacaa tcactcgcgc
     8881 attgcccgct ttataatgca gatgctcatc tgccatgcgg aacagatgac gcttatcata
     8941 aaaatgtacc gtgccgcccg gctcaaccag caaaaagcgg ttaaccgaac cagactccgt
     9001 ttgtaatgca acactgcctg caatcagcgc attgcactgc tgcgccttag ctgtcatcca
     9061 gttcactacg tcatcttgtg caagcgacga agctgccgct tccatggcaa agccgctggt
     9121 aaacatctcc ggtagaacga tcacatcgcg cccggtaata ccttccagtt gacgatcaaa
     9181 atggcgcagg ttggcaggac catccaccca caccaacggt tgctgcaaaa gcgtaatctt
     9241 caaactaggc acggtgtaca actcctttat gcgaagggtt ttataacttt aacaccttat
     9301 caggcagttg ccttagcgca gaataaattg ataacaaatg ctgatattgg aaatatctga
     9361 tttgcaaatt atcgtgttat cgccaggctt taggaggtta ataacatggg caggataagc
     9421 tcgggaggaa tgatgtttaa ggcaataacg acagtcgccg cactggtcat cgccaccagt
     9481 gcaatggcgc aggatgattt aaccattagc agccttgcaa agggcgaaac caccaaagct
     9541 gcgtttaatc agatggtgca agggcataag ctacctgcat gggtgatgaa aggcggtact
     9601 tatactcccg cacaaaccgt aacgttggga gatgagacgt atcaggtgat gagcgcgtgc
     9661 aaaccgcatg actgtggctc gcaacgtatc gctgtgatgt ggtccgagaa atctaatcag
     9721 atgacggggc tgttctcggc tattgatgag aaaacgtcgc aagagaaact cacctggctg
     9781 aatgtgaacg atgcgctttc gattgatggt aaaacggtgc tgttcgcggc gttgaccggc
     9841 agcctggaaa accatccgga tggctttaat tttaaataat tagcggataa agaaacggag
     9901 cctttcggct ccgttattca attacgcggc ttcaactttc cgcactttct ccggcaactt
     9961 taccggcttc gtcgccagct cttccggatc aaagtcatca acgttaatac tgcgcagacg
    10021 gctttcttcc gctttcacca gaatagcggc ttcatcttta tcaatcagcc ccttcgtcag
    10081 cgcgttgtgc gccagttcat ccagacgggt aaatggcaga tttttaccca gctctttaca
    10141 gatccgctga tgaatcgggt cggcggcaat cacatccacc aacgcctctt ccagtaaacc
    10201 aaccgggtta tgctcgcttg gcgtcaggta ctgaccgcga ccaatacgtg aacgagtggc
    10261 gttcggcact tgtaaaatct tcgccatttt atgatccagc ttgtcagacg gtgccagata
    10321 atgacgtccg gtcgggaaga tcaccacatt cagcagcccg gcaaccacgc ggttcgggaa
    10381 gttttgcagt aaattgtcca ttgcctgttc tgcctgatac agcgcatcct gtacgcccca
    10441 gtgcaccagc ggcagatcgg cttcattacg gccttcgtcg tcataacgct tcagaacggc
    10501 agaggcgagg taaagctggc ttaaaatatc ccccagacgt gccgagatac gctcacgacg
    10561 tttcaggctg ccgcccagca gtgccatcga gacatcagaa agcagggcga ggttggcgct
    10621 caggcggttc aggtgctgat agtagcgttt agtggcatcg ccggttggcg tgctgctggt
    10681 taaaccgcgc gtcaggccca gccagaagct gcgaactttg ttgctaccga cgtgaccgat
    10741 atgtttgaac aacagtttat cgaacgcgtt gacgtcattg ttcttcgccg cttccatctc
    10801 ttccagcacg tacggatggc aacgaatcgc tccttgtccg aagatcatca tgctgcgggt
    10861 cagaatgtta gccccttcaa cggtgatggc aatcggtgcg ccctggtaag cacgcgccag
    10921 gaagttgctt tgcccgagca taatgccttt accgccggta atatccatcg catcaataat
    10981 cgactgctgc ccgcggtggg tacagtgata cttaacgata gccgacagca cggcaggttt
    11041 ttcgccgagc ataatgccgt aggtaatcag cgatgccgca gcatccatta cataagcgtt
    11101 cccggcaata cgcgccagcg gctcttcaat cccttccatc ttaccgatag agattttgaa
    11161 ctgacggcga atgtgagaat acgcgcccgt tgccagcgct accgatttca cgccgccggt
    11221 tgagttggaa ggcagggtga tgccgcggcc taccgagagg cactccacca gcatccgcca
    11281 gccttgcccg gccattttcg gcccgccgat gatgtaatcg atcggcacga agacatcttt
    11341 accgcgcgtc ggtccgttct ggaacggtac gttcagcggg aagtggcgac gaccaatttc
    11401 cacgcccggc gtggtggttg ggatcagcgc acaggtaatg cctaaatctt ctgcaccgcc
    11461 gagtaatttt tccgggtcgg agagtttaaa cgccagccca agcacggtcg caatcggtgc
    11521 cagcgtaatg tagcgtttgt tccaggtcag acgcatcccc agcacctgct ggccctgcca
    11581 ttcgcccatg cagacaatcc cggtgtccgg aatcgcgccc gcatcggaac ccgcttccgg
    11641 gctggtcagt gcaaagcagg ggatctcctg accacgcgcc agacgcggca gatagtgatc
    11701 tttctgctcg tcagtgccgt aatgttgcaa cagttcgccc gggcctaatg agtttggcac
    11761 gccgacggta atcgccagga tcccgctcac gccagagagt ttttgcagca cacgagactg
    11821 ggcataagct gagaactcca gtccgccgta ctcttttttg ataatcatcg cgaagaagcg
    11881 atgctcttta aggtacgccc acaactccgg tggcagatcc gccagctcat gagtaatctg
    11941 gaaatcattc gccatccggc aggcttcttc taccgggccg tcgagaaacg cttgctcttc
    12001 agcggtcagg cgcggctgcg gatagttatg cagctttttc cagtccggct tgccctggaa
    12061 caagtcgccc tcccaccagg tggtgcccgc atcaatcgct tctttctcag tgcgcgacat
    12121 cggcggcatc accttacgga aaccgcgaaa taccggcgcg gaaatcatcg acttacgcat
    12181 aggcgcaaag ttaaatggca cgaggataat ggtcagaggc accagtaccc acgccgacca
    12241 cagaccagca acgccgaggg cggctgtcca ggcgagcaaa atcagactgc tgataaataa
    12301 gctcacgcgg tgatagaaca acgcgccgag caggacaacc gtagcgagaa tactcaaaat
    12361 catcataacg aaaagcccct tacttgtagg aggtctgacc acttgtgatg atatggttgt
    12421 agtggatgta aaaacattta gcaatgtgtt tacaatataa ttacaacaaa gctcacattg
    12481 ttgctgtttt tatccgcact tcaggtcaaa aagtcctggt catagcacct gcccgtactt
    12541 ctcgcttttg gcggtatccg gtacactgca ttttgtctat tacatttatg ctgaaggata
    12601 tcctcatgta ccaggatctt attcgtaacg aactgaacga agcggcggaa acgctggcta
    12661 actttttaaa agatgacgcc aatattcacg ccattcagcg cgcggcggtc ctgttagcag
    12721 acagctttaa agccggtggc aaagtgcttt cctgcggcaa cggcggttcc cattgcgacg
    12781 ctatgcactt tgccgaagag ttgaccggtc gctaccgtga aaaccgtccg ggctacccgg
    12841 cgattgctat ttctgacgtt agtcatattt cctgcgtcgg taatgatttc ggtttcaatg
    12901 atattttctc ccgctacgtt gaagcggtag gtcgcgaagg cgatgtactg ctggggatct
    12961 ccacctccgg taactctgca aacgtgatca aagcgatcgc agcggcgcgt gagaagggga
    13021 tgaaagtgat caccctgacc ggtaaagacg gcggtaaaat ggctggcacg gcggatatcg
    13081 aaattcgcgt tccgcacttt ggttatgccg accgcattca ggagattcac attaaagtga
    13141 tccatatcct gatccagtta attgaaaaag agatggttaa gtaagtctgg cgtaggccgg
    13201 ataaggcgtt tacgccgcat ccggcacttg tgcgctgatg cctgatgcga tgctgacgcg
    13261 tcttatcatg cctacaaatc tgtacccgaa ccgtaggccg aataattcgt ttacgccgca
    13321 tccgacctga aaattcttaa atcaatcttc gccggggtcc atgcgctccc gctgttgtgg
    13381 aggttaccca tgtgcgaatt gctcgggatg agcgccaacg tccctaccga tatctgcttt
    13441 agtttcaccg gacttgtaca gcgtggtggt ggaaccgggc cacataaaga tggctggggc
    13501 attacctttt acgaaggtaa aggctgtcgc acatttaaag atccacaacc cagctttaat
    13561 tcccccatcg ccaaacttgt ccaggactac ccgataaaat cctgttcggt ggtggctcat
    13621 attcgccagg ctaatcgggg cgaggtggcg ctggaaaata ctcacccatt tacccgcgag
    13681 ttatgggggc gtaactggac ttatgcccat aacggacaac tgacgggcta caaatcactg
    13741 gaaaccggca acttccgccc ggtcggtgaa accgacagcg aaaaagcctt ttgctggctg
    13801 ctgcataaat taacgcagcg ttacccgcgc acgccgggca acatggcggc agtgtttaaa
    13861 tatatcgcct cactggcgga tgaactgcgg gcgaagggcg ttttcaacat gctgctttcg
    13921 gacgggcgct atgtaatggc gtattgttcg actaatttac actggatcac ccgccgcgcg
    13981 ccgtttggcg tggcaacgtt gctggatcag gatgtggaaa tcgacttcag ctcgcagacc
    14041 acaccgaatg atgtggtcac ggtgattgcg acacagccgc tgacgggcaa tgaaacctgg
    14101 caaaaaatta tgccaggcga atggcgctta ttttgcctcg gggagcgtgt agtttgatgc
    14161 cagttgtggc tgcaccactt cgtggcttaa cggcttgctg accacgtaac ggccattggc
    14221 cacagaaacg gtcggtggct tacgggtttg ctcaaagtag tcgtagcccg gcttcagctg
    14281 ctcccagaag tccttaaagt tggaatattt atggcgcttc atattggcgt cggtcatgcg
    14341 gaatgggtaa atactcacct gcacgctcgg ctgaccaaac accagcgcac cagtaacgaa
    14401 ctggaagatc tcatcaatac cctgattggt cattgcgtag cagccgatgg atacacaatc
    14461 gccgtggatc atcaggtatt tcccttcata accatgcgca cggtcataag cattggggaa
    14521 tccaatatta atcgctttgt agtaacggct gtctggtttt aactgattac gctggacgct
    14581 ataaaaccct tccgggcttt tgaaatcgcc ctgacgctgt tttggcccta agccgccgga
    14641 atatttacag attttataac tgtcgagcag ttgatattgc tcgcccattt tgacgtaaag
    14701 atcgagcgta cgttcttcct tgaagatctg gatgtagaca ggggatccca tcaactgctg
    14761 cttatactct ttgctcactg gcgtggtcga gctactgctg cccagcaaac cggcaaacga
    14821 aacgcacggg atcaacaaca tcgcaagaat taatgcgatt ttacgcatac tgcttattcc
    14881 ttgataaaac ggttacacac gccaggacgg caaaatggat cccaaatcgg aatagtctgg
    14941 atttggaagg ctcacattat caccaaaaga gttttacgca agcctgtcgg cgctgggttt
    15001 acaaatttag cacaggggca gttttcaata gtccgaaaag cgcatgtatc atttatttcc
    15061 taatattatg aaatctcaat agtaatatgc aaataagcaa ttaataatcc cgtttactcc
    15121 tccggtcttt taaaattctc accttcattc agccggttta tgtctttcat gtcttcgttc
    15181 ttgctcggca gatttttgca tccaggcgtg ttttcgttat gcgttttgct ccctttactc
    15241 gccagcgcca ccacaaccgg gagcagataa aacagcatcc agaatggttt cctgcgccgc
    15301 tcaaggcgag tgacagacgc tggcaggcgc tggcggaaaa caaccacttt ttaagcagcg
    15361 atcacctgca taacatcacc gaagtggcga ttcaccgcct ggagcagcag cttggcaagc
    15421 cttacgtctg gggcggcacg cggcccgatc aaggcttcga ttgtagtggg ttagtttttt
    15481 atgcctacaa caagatcctt gaggcgaaac tcccgcgcac ggccaatgag atgtaccact
    15541 accgtcgggc aacgattgtg gcgaataacg acctgcgccg gggagatttg ctatttttcc
    15601 atatccacag ccgcgagatt gccgaccata tgggggtgta tctgggcgac ggtcagttta
    15661 ttgagtcact gcgcaccggg gaaaccattc gggtaagccg gttagccgaa cctttctggc
    15721 aggaccattt tttaggcgcg cgcaggattt tgacggaaga gatgattttg taggccggat
    15781 aaggcgttta cgccgcatcc ggcggttgtg cacggatgcc tgatgcgacg ctgacgcgtc
    15841 ttatcatgcc tacaaatctg tgcgtttttg cgagccgctt tcccgatata acacagcgtc
    15901 attgtcggcc tttccttttc ctcttaccgt tgagaaaaag gagtcgctat gtcggaatat
    15961 cgtcgttatt acatcaaggg gggaacatgg tttttcacgg taaatttacg caatcgtcga
    16021 agccatcttc tgaccaccca gttccagacg ctccgtaacg ccattattaa cgttaagcga
    16081 gacagacctt ttgaaattaa cgcctgggtc gttttgccgg agcatatgca ttgcatatgg
    16141 acattacccg aaagcgatga tgatttttcc tctcgctggc gggagattaa aaagcagttc
    16201 acccatgctt gcggattgaa aaatatctgg caaccgcgtt tttgggagca tgccattcgc
    16261 aatacaaaag attatcgcca tcatgttgat tatatttata taaatccaga gaaacatggc
    16321 tgggtaaagc aggtgagtga ttggccattc tcaacgttcc atcgcgatgt cgcgcgaggg
    16381 ttatatccca tcgattgggc gggagacata acggatttaa gtgccgggga gcgtatcatt
    16441 ttataactgt gcgctggtgc cggatgcgct tacgcttatc cggcctacaa atcgtgcttt
    16501 aacggtaggc cggataaggc gttcacgccg catccggcag ttatgcgcag atgcctgatg
    16561 cgacgctggc gcgtcttatc atgcctaaaa acttgtgcct gaacggtagg ccggataagg
    16621 cgtttacgcc gcatccggca attgtgcacc gatgccggat tcttacatca acgcccccat
    16681 aatcttcaac tccagctcat ccggcacttc gttatacgac agcacatgca gccccggcgc
    16741 aaacaacctt gcataacgcg gcagcaaagg gcgtagctgc ggcggcacca gcagcaccgg
    16801 atctttcccc gccgctttca tctgctcttt cacctgtggc atcgtgctct ggaactggtt
    16861 gagcatattc gggtcaaccg gcacgctgtc gagcatcact ttgccggcct gttgcgcctg
    16921 attcactacg ttggtcagca gattttccag ctcattattc agcgtataca ccgtcagctc
    16981 ctgcttgcga acgaacggat gagtaatgct gcgccgcagc gccaggcgca catcggccgc
    17041 cagcagaata tgatctttag tcaccgtgct actggcgacc agcacggtgg cgatggtgac
    17101 gatatcgcgc agggaaacgc cttcggtcag cagcgcccgg tacactttca gcaattggct
    17161 gtaattgagc gccgcgctta aatcctccgc caggcgcggt gccatcgacg acaaacggtt
    17221 atgcaactgc gtgatgtcat catagttaaa caaatcagga atatagctgc gcacaatctt
    17281 gttcacatgg gtggcaatca cactggcgct gtcgatcacc tgatacccca tattcagagc
    17341 cttcgctttc tgcgcaggct ggatccaggt gaccggtatc ccgtacgccg ggtcgttccc
    17401 cagcacgccg tcaatctcgc cgtaggtttc gctggagggc agcgccatca gtttatccgc
    17461 cggaatatcc gcttcatcag cctcaatgcc gttgatgaaa atagcgtact ggctgggctt
    17521 gaggcggaag ttttcccgaa tgcggatctc cggcagcagc acgccgttac cgtcggaaat
    17581 cacctgccga acgccgcgaa tccgctgggt gagcgggttg ccctgcgctt tgtccaccag
    17641 cgccaccagc ttgtagccga ggcttaagct gatgggctcg atcagcggaa tagtttccca
    17701 actgacctgt tgttcgctgg tttcagtgat ggtgcgggtc agcgtttcga ggcttttctc
    17761 ttccgcctcc gccgcctgcg ggcgtttgct catccgccag ccggtaaagc caagcagggc
    17821 gctgaacagc aaaaacggca gatgcggcat ccccggcacc accgccagca cgaacatgat
    17881 cccggtggcg gtgtagagca ccgacgggct tgccagcaac tggtggcgca cgtcatgggc
    17941 gatatcgccg ttgtcgctga cgcgggtgac gataatcgcc gccgcggtgg agagcagcag
    18001 ggaagggatc tgcgccacca ggccgtcgcc gatggtcatc agcacgtatt gctggaaggc
    18061 ggcgtcggcg ctcaggttgt atttgaagat gccgatacag acgccgccgc tcaggttgat
    18121 cgccagaatc atcatcccgg cgatggcgtc tccgcgtaca aacttcgatg ccccgtccat
    18181 cgcgccgtag aaatcggcct cgctggcaac atctttacgc cgggtttgcg cctgcgcctg
    18241 gttgatcaat ccggcgttaa gatcggcgtc aatcgccatc tgtttgccgg gcatcgcgtc
    18301 cagggtaaag cgggcagaaa cctcggaaat acgctcggcc tctttggtga cgacgataaa
    18361 gttgattgtc aacagcgtca gtaaatccga gcgtgaaagc attatcgccg cgctgcacgg
    18421 gcagtcgatt tttaacggtg gtgggctgtc gccgttaaat aaaatcagcc cgtctcatcc
    18481 gccaaaacct gccaccgtgg cggtgccgga agagacggag aaaaaagcgc gtgatgtgaa
    18541 tgagaaaacg gcgctgctga agaagaaatc cgccaccgag cttggcgagt tggcaaccag
    18601 tatcaacacc attgcccgtg atgcgcatat ggaagcgaat ctggagatgg agattgttcc
    18661 ccagggatta cgcgtgctga ttaaagacga ccagaatcgc aatatgttcg aacgcggcag
    18721 cgcgcagatt atgccgttct ttaaaacttt gctggtggag ctcgcgccag tgttcgactc
    18781 gctctataac aaaattatta ttaccgggca taccgatgcg atggcctaca aaaacaatat
    18841 ctacaacaac tggaaccttt cgggcgatcg cgcgctttca gctcgtcggg tgctggaaga
    18901 ggccggaatg ccggaagata aagtgatgca ggtaagcgca atggcggacc agatgctgct
    18961 ggacgcgaag aatccgcaaa gcgcgggcaa tcggcgtatt gagattatgg tgctgaccaa
    19021 aagtgcgtcc gatacgctgt atcaatactt tggtcagcat ggggataaag tggtgcagcc
    19081 gctggtgcaa aagctggata agcagcaggt gctttcgcag cgaatgcgct aaatgctgaa
    19141 tctttacgca tttctcaaaa cctgaaatca ctgtatactt taccagtgtt gagaggtgag
    19201 caatgcgtaa aatcattcat gtggatatgg actgcttttt cgcagcggtg gagatgcgcg
    19261 acaatcccgc tctgcgcgat atccctattg ctattggcgg cagccgcgaa cgtcgggggg
    19321 tgatcagcac cgccaattat cccgcgcgta aatttggcgt acgtagcgct atgcctactg
    19381 ggatggcgct caaattatgc ccacatctca ccttgcttcc ggggcgcttt gacgcctaca
    19441 aagaagcctc aaatcatatc cgtgaaatct tctcgcgcta cacctcgcgc attgaaccgt
    19501 tgtcactgga tgaggcttat ctcgatgtca ccgatagcgt ccattgccac ggttctgcga
    19561 ccctcatcgc ccaggaaatc cgccagacga tcttcaacga gctgcaactg acggcatctg
    19621 cgggcgtggc accagtaaag tttctcgcca aaatcgcctc cgacatgaat aaacccaacg
    19681 gtcagtttgt gattacgccg gcagaagttc cggcattttt acaaacctta ccactggaaa
    19741 aaatccccgg cgtcggcaaa gtctcggcgg caaaactgga agcgatgggg ctgcggacct
    19801 gcggtgatgt acaaaagtgt gatctggtga ttctgcttaa acgctttggc aaatttggcc
    19861 gcattttgtg ggagcgtagt caggggattg acgagcgcga cgttaacagc gaacggttac
    19921 gaaaatccgt cggcgtggaa cgcacgatgg cggaagatat tcatcactgg tctgaatgtg
    19981 aagcgattat cgagcggctg tatccggaac ttgaacgccg tctggcaaag gtaaaacctg
    20041 atttactgat tgctcgccag ggggtgaaat taaagttcga cgattttcag cagaccaccc
    20101 aggagcacgt ctggccgcgg ctgaataaag ctgacttaat cgccaccgcg cgtaaaacct
    20161 gggatgaacg ccgcggcggg cgcggtgtgc gtctggtggg gctgcatgtg acgttgcttg
    20221 acccgcaaat ggaaagacaa ctggtgctgg gattatgatg tatactatta tgtatattct
    20281 ggtgtgcatt attatgaggg tatcactgta tgcatcgaat tctcgctgaa aaatcggtca
    20341 atatcactga gttacgtaaa aacccagcta aatactttat tgatcaacca gttgcggttc
    20401 tttctaataa tcgccccgca ggatatctct taagtgccag cgcattcgaa gcgttaatgg
    20461 acatgcttgc tgaacaagag gagaaaaagc ccataaaggc gcgctttcgt ccaagtgctg
    20521 caagattaga ggaaattaca cgccgcgcta aacaatatct taatgatatg acggatgatg
    20581 atttcaatga ctttaaggaa taaggatgcg ggtattcaaa acaaaactta ttcgcctgca
    20641 acttacagca gaggaacttg atgcgttaac ggcggatttt atttcctata agcgtgacgg
    20701 tgttttgcca gatatatttg gtcgcgatgc actctacgac gactcgttta cctggccatt
    20761 aatcaaattt gagcgagttg ctcatattca tctggcaaat gtgaataatc catttccgcc
    20821 acagttgcgc caattcagca gaacgaatga cgaagcgcat ttggtatatt gtcagggtgc
    20881 gtttgatgag caagcatggt tgctcattgc cattctgaag cctgaacctc ataaactggc
    20941 tcgagataac aaccaaatgc ataaaattgg aaaaatggca gaagcgtttc gcatgcgttt
    21001 ttgaatttat attatgaata acatacaaat aagagattat cagcctggcg attttcagca
    21061 actatgcgct attttcatta gagcggttac gatgaccgtc agtcagcatt attcactaca
    21121 acaaattgcc gcctgggcgc agattgacga atctcgctgg aaggagaaac tcgcgaaatc
    21181 acaagtgcgg gttgcagtta ttaatgcaaa actggtcggc tttattacct gcgttgaaca
    21241 ctatatcgat atgttatttg ttgaccctga atacacccgc cgtggggttg ccagcgcttt
    21301 gttaaaacct tttattaagt ctgaatccga acttacggtg gacgcaagca taaccgcaaa
    21361 accctttttt gaacgttatg gttttcagac agttaagcag cagcgcgttg aatgccgggg
    21421 agcgtggttt attaattttt atatgcgtta taaaccgcaa cattaaatcc agcttgcaat
    21481 gaaaataacg cccgcctagt atgtgcgcgt tttcttattc cacaaactgc aaggaggtaa
    21541 atcatgacaa atcctttatc aatgactctt tgcagacctt tccaggatta attctttttt
    21601 tcttgccctg gattcgtctg ccatttcctg attttttaat tgttactctc aataattcaa
    21661 gtcgcagcac atgcaacttg aagtatgacg agtctattta cggaatggta tatgggcaat
    21721 tatattcgtc ccttatccga tgcggtattt accatcgcat ctgatgacct gtggatcgag
    21781 agtttagcga tccaacaatt acacaccacg gcaaatttac ccaacatgca gcgcgtagtg
    21841 gggatgccag atttacaccc cggacgcggc tacccgattg gcgcagcgtt cttctccgca
    21901 ggccgttttt acccagcact ggtcggcaat gatatcggct gtggcatggc gctatggcaa
    21961 acagatattc tcgctcgcaa atacaacgcc gataagtttg aaaagcgatt atcagctttg
    22021 gatgacgttg ctgaagaaag ctggctggag gaaaacctgc cgtcagcatt tgcacagcat
    22081 ccgtggcgca gctcgcttgg ctctatcggt ggtggcaatc atttcgctga actgcaacaa
    22141 gttgatcaaa tcatcaacgc tgaactgttt gcactggcag gtctggatgc gcagcatctg
    22201 caactgctgg ttcatagcgg ctcgaggggg ctagggcagt ctattttaca gcggcatatt
    22261 gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg acgcgctggc gtttgcgcgt
    22321 tttaatcgcc acctgatcgc tttgcgcata atgcaacagg ttaaggccac cggaagtccg
    22381 gttctggatg tggcgcataa ctttgttagc gcgtgtcgaa tcggtgatca acaaggggtc
    22441 ttacatcgta aaggtgccac gcctgatgac tgtggtctgg tggttattcc cggttcacgc
    22501 ggtgattact ccaggctggt tcagcccgtc aggagtgagg aaacattgca ttcgctggcg
    22561 catggggctg ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac
    22621 acagcgacgc aactctcgcg tactgaactt ggcagtcggg taatttgtcg cgataaacaa
    22681 ctcatcttcg aagaagcgcc acaagcctat aaatcggctg aaagtgtggt gcaatgtcag
    22741 ggcaggccgg attaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg ctcaaaaaca
    22801 gtggagggaa aaaaggatga tcttgctata actctcctca gcgcaggggc cggaagaatg
    22861 ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc gacaagacgt
    22921 cgcggtaacg gtgctggaaa cagaaacagg ccgctactct gacacgctgc gttcggcgct
    22981 gatttctctg gatggcgaca acgcatgggc gttaagcgaa agctggtgtg gcactattca
    23041 gtggatttgc ccgagcccgt atcgcccaca tcacgggcgt aaaaactggt ttctgggcat
    23101 tgggcgtttt accgctgatg agcaggagca atccgatgcg atccggtatg agacgctgcg
    23161 ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac gcgccacgca
    23221 tttggcatcc ggtattagcg tgaaggttca gtcagagcgc agccagcatg ctaacaaacg
    23281 gctggcgcga ttgctgattg cctggaagct ggatcagcag caataggaaa atagcgcggt
    23341 gctgaaatca cagcggcgaa tgttccatca ccagattgaa cgtggcaacc cgcgacgaac
    23401 gtttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta
    23461 aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt
    23521 cagcagtgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg gagagtgtgg
    23581 accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttgaacag
    23641 accacattcc aggcccgcgt ggataatctg gatgttcggc gtcttgttga acaggcgctg
    23701 ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata
    23761 tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc agcgaatcca gcatgctcac
    23821 cacgtagtct ttaccgctgt cgattagtga acggatcagg cagtgaattt ctacgttatt
    23881 gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt ttgccacgtc
    23941 ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga
    24001 tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattttt
    24061 ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca gagatttcag
    24121 tgcgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt
    24181 acgcagtgtg ccgccgttga aatcgataag gcgcaggtcc agttcttctg catgacccgc
    24241 caggaagcgc accagcagtt tgttggcatt acccaggcca acgtggattt caccgccgga
    24301 gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc
    24361 ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac ccatgtagat
    24421 ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg
    24481 taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg
    24541 gccgtgaacc acgttttcgt cagccagaac cgccagtgca gaggccatac caatgccgtt
    24601 atccgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat
    24661 aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg
    24721 ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt gacgaatcag
    24781 gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat
    24841 gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa
    24901 aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt
    24961 cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa
    25021 gacaggcttg cgagatatgt attgagaata ccactttatc ccgcgtcagg gagaggcagt
    25081 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
    25141 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
    25201 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
    25261 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
    25321 ggtctggtac cgggggcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
    25381 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
    25441 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgctgtt
    25501 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
    25561 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat cgaacagccg
    25621 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
    25681 gcattgccgg gcgttgttct ttttaacttc aggcaggtta caatagtttc cagtaagtat
    25741 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
    25801 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
    25861 gcagtcggct cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
    25921 gtggatctgg cgcggtattg acccacgtga aatcctcgac gtccaggcac gtattgtgat
    25981 gagcgatgct gaacgtaccg acgatgattt atacgatacc gtgattggct accgtggtgg
    26041 caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag cctgtacgga
    26101 agaagatccg caactcagtg gtcgacactg gctgcatgcg gctacgttgt acaacattgc
    26161 cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt tatcaaaccg
    26221 cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt
    26281 acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc
    26341 gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga
    26401 acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt
    26461 ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg tcttaaaggc
    26521 gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg
    26581 cgctaacgtt gccgtgcgtc tggcatacct tgaatcgccg cgtctgaaag cggttgcctg
    26641 tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga
    26701 aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt
    26761 acgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc
    26821 aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg
    26881 cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta
    26941 tcggaatttt gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta
    27001 aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg agatagcaat
    27061 gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc
    27121 gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg
    27181 cgtcaacgtg aaaccggcac cggaagtgcg tgaattctgg ggctggtgga tggagctgga
    27241 agcgcaggaa tcccgtttta cctacagcta ccagtttggt ctgttcgata aagcaggcga
    27301 ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc acaccctgcg
    27361 tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc
    27421 gaatgatttt cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgcat
    27481 cacatccggc aatattcatt aaaactgata cgtcatccca accgcgacag tatcatcatt
    27541 attaatattc agcttattat cgctatccag ttggttgatt ttataatcaa caaacgctga
    27601 catatttttg ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc
    27661 accgatgccc tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc
    27721 aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc
    27781 agttattggc gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc
    27841 atcgtatttc agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag
    27901 gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc
    27961 gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg
    28021 gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa
    28081 gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg
    28141 cgccgaggaa tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa
    28201 cgcgcccagg ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa
    28261 agcgagacgc gttttttgct gtgcagtatc actctccgct ttatttccgg caaactccgc
    28321 ttcccaccga ccataaccag tcagttgatc gttaatttga gtttcacctt taaaaccaaa
    28381 acggatataa ctctggtcgc catctttact ggcgttatca ctcatataat gcatggcttt
    28441 aactttgcca tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac
    28501 agatgcagat gccacaatgc ccatcaccac taatgccaga g
//
LOCUS       DAJTPG010000046        28396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTPG010000046 DAJTPG010000000
VERSION     DAJTPG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..132)
                     /gene="yjdI"
                     /locus_tag="OO230_002093"
     CDS             complement(<1..132)
                     /gene="yjdI"
                     /locus_tag="OO230_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5691849.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     "
     gene            313..1944
                     /gene="dcuS"
                     /locus_tag="OO230_002094"
     CDS             313..1944
                     /gene="dcuS"
                     /locus_tag="OO230_002094"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5691850.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1941..2660
                     /gene="dcuR"
                     /locus_tag="OO230_002095"
     CDS             1941..2660
                     /gene="dcuR"
                     /locus_tag="OO230_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5691851.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3231..4571
                     /gene="dcuB"
                     /locus_tag="OO230_002096"
     CDS             3231..4571
                     /gene="dcuB"
                     /locus_tag="OO230_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5691852.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4649..6295
                     /gene="fumB"
                     /locus_tag="OO230_002097"
     CDS             4649..6295
                     /gene="fumB"
                     /locus_tag="OO230_002097"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5691853.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6418..7047
                     /locus_tag="OO230_002098"
     CDS             6418..7047
                     /locus_tag="OO230_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5691854.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7186..8607)
                     /gene="melB"
                     /locus_tag="OO230_002099"
     CDS             complement(7186..8607)
                     /gene="melB"
                     /locus_tag="OO230_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5691855.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8710..10065)
                     /gene="melA"
                     /locus_tag="OO230_002100"
     CDS             complement(8710..10065)
                     /gene="melA"
                     /locus_tag="OO230_002100"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5691856.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10348..11256
                     /gene="melR"
                     /locus_tag="OO230_002101"
     CDS             10348..11256
                     /gene="melR"
                     /locus_tag="OO230_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5691857.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11455..13722
                     /gene="adiA"
                     /locus_tag="OO230_002102"
     CDS             11455..13722
                     /gene="adiA"
                     /locus_tag="OO230_002102"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5691858.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14047..14808
                     /gene="adiY"
                     /locus_tag="OO230_002103"
     CDS             14047..14808
                     /gene="adiY"
                     /locus_tag="OO230_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5691859.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14945..16282
                     /gene="adiC"
                     /locus_tag="OO230_002104"
     CDS             14945..16282
                     /gene="adiC"
                     /locus_tag="OO230_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5691860.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16386..18029
                     /gene="eptA"
                     /locus_tag="OO230_002105"
     CDS             16386..18029
                     /gene="eptA"
                     /locus_tag="OO230_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5691861.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18026..18694
                     /gene="basR"
                     /locus_tag="OO230_002106"
     CDS             18026..18694
                     /gene="basR"
                     /locus_tag="OO230_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5691862.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18695..19795
                     /gene="pmrB"
                     /locus_tag="OO230_002107"
     CDS             18695..19795
                     /gene="pmrB"
                     /locus_tag="OO230_002107"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5691863.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19771..19860)
                     /gene="pmrR"
                     /locus_tag="OO230_002108"
     CDS             complement(19771..19860)
                     /gene="pmrR"
                     /locus_tag="OO230_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5691864.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19972..21474)
                     /gene="proP"
                     /locus_tag="OO230_002109"
     CDS             complement(19972..21474)
                     /gene="proP"
                     /locus_tag="OO230_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5691865.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21738..22616)
                     /locus_tag="OO230_002110"
     CDS             complement(21738..22616)
                     /locus_tag="OO230_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5691866.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22613..24841)
                     /gene="crfC"
                     /locus_tag="OO230_002111"
     CDS             complement(22613..24841)
                     /gene="crfC"
                     /locus_tag="OO230_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5691867.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25244..25579
                     /locus_tag="OO230_002112"
     CDS             25244..25579
                     /locus_tag="OO230_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5691868.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25737..26180
                     /gene="yjdN"
                     /locus_tag="OO230_002113"
     CDS             25737..26180
                     /gene="yjdN"
                     /locus_tag="OO230_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5691869.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26313..27101
                     /gene="phnC"
                     /locus_tag="OO230_002114"
     CDS             26313..27101
                     /gene="phnC"
                     /locus_tag="OO230_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5691870.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27126..28142
                     /gene="phnD"
                     /locus_tag="OO230_002115"
     CDS             27126..28142
                     /gene="phnD"
                     /locus_tag="OO230_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5691871.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28248..>28396
                     /locus_tag="OO230_002116"
     CDS             28248..>28396
                     /locus_tag="OO230_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5691872.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7295 a         7416 c         6933 g         6752 t
ORIGIN      
        1 tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct gacatatcgc
       61 agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc cgtccagtag
      121 cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag catagccccg
      181 gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta tatagccaat
      241 catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga aatttgtcac
      301 acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg tccgatgaaa
      361 ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct attggtggtg
      421 catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc caacaaggca
      481 ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt gcagaaaaaa
      541 ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa cgatctgctg
      601 tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc ccagcgtatt
      661 ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga aaatgtcgct
      721 atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta cgatgaaaat
      781 cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac ccaacagatc
      841 aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt cggactgatt
      901 ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga accctacgaa
      961 atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga aggcgtcgtt
     1021 gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga attgctgaat
     1081 taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg gtcacaggtg
     1141 gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga gattacgatt
     1201 aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt tatcatcggt
     1261 gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg actcgacggt
     1321 ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa taaattgcat
     1381 gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta cattctcaaa
     1441 acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa atctccggtt
     1501 atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca tacgctgatt
     1561 ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc gacgctgatt
     1621 accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga acccggtggc
     1681 gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt taacgatgat
     1741 ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt ctcgacaaaa
     1801 ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa tctcggcggc
     1861 agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca gataccctgg
     1921 gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac gcaatggtcg
     1981 cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt ggaacagcct
     2041 cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc gacctgatat
     2101 tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc ctgcataacg
     2161 cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca accattaaag
     2221 attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct tcccgctttg
     2281 aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat cagtattatg
     2341 accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag gatcctcgtc
     2401 gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg attgacgcgc
     2461 atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc tcgcgtgttt
     2521 cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc agtatccatt
     2581 atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac tactcactgc
     2641 tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt attaccgtaa
     2701 tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc cagatcaaat
     2761 ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg gtgtttttaa
     2821 tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg tgtattttta
     2881 cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc tgagaacaaa
     2941 aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat ctgcgcagta
     3001 caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt atcacagtgc
     3061 ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc taaagccggt
     3121 aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac agaacgtgac
     3181 tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac atgttattta
     3241 ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag ggtggtatcg
     3301 cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc caccttcagc
     3361 caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg gcatcggcga
     3421 ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag ctgctgcgcc
     3481 gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg accattcttt
     3541 gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc gccattaaga
     3601 acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag atggggatta
     3661 tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt aatgtcacct
     3721 ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca tcgacgttaa
     3781 tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg gataaagacg
     3841 aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac ggtgataccg
     3901 cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg attttcctcg
     3961 gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca tccttcggcg
     4021 gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc ggggcgctga
     4081 ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc ttccgttccg
     4141 gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc atgttcggtg
     4201 cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat ccgtgggcgt
     4261 atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg gcgctggcgg
     4321 cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg gcttcagcac
     4381 cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca gcgattcagt
     4441 ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt tttattctgc
     4501 cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc gcgatgtacg
     4561 ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca ttttcgaata
     4621 acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct accaggcacc
     4681 tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt acgttagcgt
     4741 tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga ccctgctggc
     4801 gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga aacaggttgc
     4861 ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac tgcaattctt
     4921 aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata ccggcaccgc
     4981 gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg aagaagcgct
     5041 gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc aaaacgcggc
     5101 gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa tcgacctgta
     5161 cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg gctctgccaa
     5221 caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac tgaaaaactt
     5281 cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc atatcgcgtt
     5341 tgtgattggc ggtacgtctg cggaaaacaa cctgaaaacc gtcaaattag caagcgctca
     5401 ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc gcgatgtcca
     5461 gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc agtttggcgg
     5521 taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg catcctgccc
     5581 ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa tcaaccgcga
     5641 aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac aagaactgcg
     5701 tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga aagagatcct
     5761 cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca ccattattgt
     5821 gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta aagaacttcc
     5881 gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc ctgccggtta
     5941 tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg tggatctgct
     6001 gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc agcaggttac
     6061 cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc cggcggcggt
     6121 actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc tgggtatgga
     6181 agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg atgacaaagg
     6241 taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta agtaacctct
     6301 tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct cttttgcgat
     6361 tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga actcaccatg
     6421 acccgcacac tcaagccgtt aattcttaac actggcgcac tggcgctaac gttaatcctg
     6481 atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt gacaccggtg
     6541 attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac gctgctgctc
     6601 tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta cacctacgcg
     6661 aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa tccgtatgac
     6721 aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga aattctcgtt
     6781 cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg cgtggcgctg
     6841 gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat gggccaggga
     6901 gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga tatgttctgc
     6961 gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg ccgccagcta
     7021 cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg gatgcggcgt
     7081 gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa gacgtggcct
     7141 gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg ctttcacatc
     7201 actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt tgcgatattt
     7261 atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat agaaacggaa
     7321 gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa taaactgcat
     7381 acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca tccctaacac
     7441 aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag tctgcacgga
     7501 gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa cggtatctgc
     7561 caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca aaatacccgc
     7621 aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga gaacaccaca
     7681 gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata atgatttaac
     7741 taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg acagataata
     7801 ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa atccggtaat
     7861 aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa gctgatcgtt
     7921 tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag cagacgattg
     7981 attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg aaacaataaa
     8041 aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc caccgccgac
     8101 ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac tggcaaaaaa
     8161 acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg ttggaaccag
     8221 cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt aggtcacgca
     8281 aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga gaaataagat
     8341 tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc atcgcgatcg
     8401 cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga tcctcgccac
     8461 cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt agtaatacat
     8521 gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa atccataact
     8581 gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca acgcccgcct
     8641 tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc agtagcgttt
     8701 agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg caatcaggtc
     8761 gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg ggtccatcat
     8821 cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca gcagcgtctg
     8881 tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac cgactttggt
     8941 cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc cttgtggcag
     9001 gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct cgccagtcca
     9061 gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa tccgggaggc
     9121 gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc agcgtttcgg
     9181 gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta gcttaataaa
     9241 ccacggcgta tattcggcaa aatgttctga tgattccgtg acgaagtagc ccagcttttt
     9301 gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat ttggcttcgg
     9361 tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt aactgccgtc
     9421 ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg cgcaacggta
     9481 acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg ttccctgcac
     9541 cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg cccaggtatt
     9601 catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt ccgtcatgtt
     9661 ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac cgcccggccc
     9721 caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga aatcagtcac
     9781 cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg catcctctaa
     9841 ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg aatccatcag
     9901 cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca tcagggcaat
     9961 atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt taacgaaaat
    10021 cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga tctcctggct
    10081 tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct tccaggttga
    10141 gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt atgggaataa
    10201 attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct cgcccctctg
    10261 ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc tgccataatt
    10321 ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg cagcagcgac
    10381 gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat ggagattgag
    10441 tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga agtgaatgtg
    10501 cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat caatcagggt
    10561 catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg aacctgtcag
    10621 agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct ggataaagac
    10681 ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca gcaacttagc
    10741 ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca aattcgccag
    10801 ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag ctgggaaccg
    10861 attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca tgcgcaattt
    10921 tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct gaccatcaac
    10981 gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt ccagcgggtc
    11041 atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt tcgcgcgtta
    11101 ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt tcgttcaagt
    11161 agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca ataccgcaaa
    11221 cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta tcagccaaaa
    11281 aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca cataacgtat
    11341 tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac agcccgaaaa
    11401 ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc aatgatgaaa
    11461 gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa cgccgttgag
    11521 cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac ctcctttgat
    11581 gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt cagctatcaa
    11641 atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct tcatgagcgc
    11701 caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc cgcaatggac
    11761 cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac cgccgacttt
    11821 atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt gccgccactg
    11881 ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc gccaggccac
    11941 cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga ctactatggt
    12001 gaaaatctgt tccgtaccga catgggcatc gaacgaactt ccctcggttc tttgcttgac
    12061 catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg tgccgatcgc
    12121 tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca ggcttgcatg
    12181 accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga acaaggtttg
    12241 atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta cggcattatc
    12301 gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat cagtgaaagc
    12361 ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac caactgcacc
    12421 tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa aacctccgat
    12481 cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat ctatgccgat
    12541 cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt cgccacccac
    12601 tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt acgtgaaggt
    12661 cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc caccacctcc
    12721 ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga cggcaacagc
    12781 ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca ggcgatggcg
    12841 cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg gaacaaagaa
    12901 gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc aaccaaactg
    12961 ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca cggcttcaaa
    13021 gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct tgctccggga
    13081 atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt cactgcctgg
    13141 cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat gttcctgttc
    13201 tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg ctccttcaaa
    13261 caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt tgaacaatat
    13321 cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt tgcctggctg
    13381 aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc ggtggcggaa
    13441 atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact ggtatccatt
    13501 gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc aggaatcccg
    13561 atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag ttatttacgc
    13621 tcgctgcaat cctgggacca ccatttccct agatttgaac acgaaactga agggactgaa
    13681 attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt ccgctgaagg
    13741 cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta tggcaacgtt
    13801 ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca ccccacgctt
    13861 ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac tataaaaatt
    13921 tgtaatatta gtaaaactcg ttatttttat gcatgtttat attcatcatg caattatata
    13981 accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc actcaggagt
    14041 acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc
    14101 tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat ggcgttatta
    14161 aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt tgctcatatt
    14221 agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca
    14281 gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc agacgtcttt
    14341 cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa ggaacgaaca
    14401 cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt cctttcatta
    14461 atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat
    14521 attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg
    14581 ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc
    14641 atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag
    14701 tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg
    14761 acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt tttaacctta
    14821 acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa ttcaactatc
    14881 gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt
    14941 aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca ccctgatggt
    15001 gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg cctccactgg
    15061 cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc tctcgatggt
    15121 atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt acgcccgccg
    15181 ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat
    15241 cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct tcccgattct
    15301 gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct
    15361 gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg tgctggcgct
    15421 gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg aaacctatat
    15481 ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta cccttaacgt
    15541 tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa
    15601 cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg ccgtttgcta
    15661 tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc
    15721 ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg ccattgtttc
    15781 cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc tggcgggtca
    15841 aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa
    15901 agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct
    15961 cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg tctcggtcat
    16021 ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg gccacggtca
    16081 ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc tctactgcat
    16141 ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca ccctgatggt
    16201 catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt atcccttaga
    16261 tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc caaccttaag
    16321 aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca
    16381 ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc tggctattgt
    16441 tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag gtgttgcagg
    16501 cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg gttgtcgctt
    16561 tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat cgaccactgg
    16621 cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact tacggcatcg
    16681 tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa agttatgcgc
    16741 tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct gagctgattg
    16801 cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt ttccgtggag
    16861 ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt tataaagact
    16921 acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc tctaacagca
    16981 ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg ctggtgcgaa
    17041 ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa aatttgacca
    17101 tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc tacccgcgtg
    17161 aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc gcatcttgcg
    17221 gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt gagcactaca
    17281 aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga gcgggcatca
    17341 acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc gtgcctcacc
    17401 agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc tatgacgaag
    17461 tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc gtgattgtct
    17521 tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg cagttcagga
    17581 aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag caactggtga
    17641 acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc
    17701 ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat
    17761 cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc
    17821 aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg tatcaggttg
    17881 accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct
    17941 ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg gatgatattc
    18001 tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg atacgctgtt
    18061 attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg gcgtgacaac
    18121 cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg tactggattt
    18181 agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga aaaaatacac
    18241 cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg ccgggctgga
    18301 tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac acgcccgtat
    18361 ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg ttggcaatct
    18421 gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga ttctgacgcc
    18481 caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg tacatcggga
    18541 aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca ccctggaagt
    18601 gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggctt
    18661 tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt ttctgcgccg
    18721 acctatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga
    18781 gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca
    18841 ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt
    18901 cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta tctgctatca
    18961 ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg aagcgcgcac
    19021 cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg aagcggtggt
    19081 ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg aaaggttgtt
    19141 taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga
    19201 actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc ttgatcagat
    19261 gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg
    19321 taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaactcag
    19381 taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg ccgacatcac
    19441 cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca
    19501 tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg gcggagcggt
    19561 catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg agttgagtaa
    19621 agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa gtattgtcag
    19681 ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag agacttccgg
    19741 cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga tataaagaaa
    19801 gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac ggtttttcat
    19861 tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa
    19921 gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag cttattcatc
    19981 aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg
    20041 gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc
    20101 ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg ccgtctcttt
    20161 catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat aggcaggcat
    20221 catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca gaccggcaac
    20281 cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg tcgggaacat
    20341 cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg ccagcatcag
    20401 taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg ccgggatcgc
    20461 caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa aacggtcact
    20521 cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga taataatcag
    20581 caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag
    20641 caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca acaggctgcg
    20701 ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg
    20761 gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt ccagcgcatg
    20821 gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa tacgccagcc
    20881 ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca cgcccgcacc
    20941 cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc cacgtttacg
    21001 gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac cgaccgagaa
    21061 accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta
    21121 ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag
    21181 gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc caagcggtcg
    21241 aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc tggggtcagc
    21301 ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcaaa
    21361 ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccgtcatc
    21421 aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca gcatagcttt
    21481 cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag
    21541 gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct
    21601 ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt
    21661 ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt ttaggactca
    21721 ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac cattgccccc
    21781 accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg ccagcgttgg
    21841 gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag ccaacttaaa
    21901 aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg atggtaatag
    21961 tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc tttctcgcgc
    22021 aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc atcgccaaat
    22081 gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga ccaggcgtta
    22141 tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc ctgcaccagt
    22201 aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa ccactcatac
    22261 aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg atccattttt
    22321 tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg atccagcatc
    22381 gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa catgccgccg
    22441 ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcatcagtcg ttcacgaaac
    22501 tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc cactaaaaag
    22561 cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt ggtcaatatc
    22621 gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg gtgtaaatcc
    22681 agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc accgattcat
    22741 tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc aaacatgaag
    22801 cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc tgctctgcca
    22861 gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct cgttgcaaca
    22921 cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag tcgggctgat
    22981 tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc tcgtccgttg
    23041 cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg ggaactacgc
    23101 cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat tccattttga
    23161 cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg gattccatct
    23221 cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt aacagtgtct
    23281 cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag gcggctaact
    23341 gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc agggccagct
    23401 cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat agctgcaaac
    23461 tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt aacccgtgcg
    23521 cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta tgcgcagcag
    23581 atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc tgaattggct
    23641 gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt tcgaggtccg
    23701 ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct tccacccagc
    23761 gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc gcccgattcg
    23821 ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata cagcctttca
    23881 tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta cgatcctgtt
    23941 gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc aaaatcgcct
    24001 cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc gccagtaccg
    24061 ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc ccggcttcat
    24121 ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc cccgccagat
    24181 ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca gaaaaaggaa
    24241 aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc agacaatgaa
    24301 aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg atccgctgca
    24361 taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta atatcgcaat
    24421 cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca tgtgaaaaat
    24481 gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc gcagtcattg
    24541 ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg gttgatttcc
    24601 ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt ttacacaact
    24661 cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt aaagccagtt
    24721 gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc aactgttggc
    24781 gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc tgtgtgtaca
    24841 tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt gtttttgagt
    24901 tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg tgttgttttt
    24961 ttatcagaat aaataacgtc ttctgatgcg tttaaaacgt cagaaagata aaaatatcat
    25021 gtgaataaaa aaaagaacaa gtagagcatt aacattatct taaataataa atagaggcaa
    25081 aaagattatt ttctttttgc gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc
    25141 agtacccccg acaaactcag ggattttgtg tataattgcg gcctttttcg gcaatctgcc
    25201 gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat taccacactg
    25261 cccaaaatgc aactccgaat acacttacga agataacggc atgtacatct gcccggaatg
    25321 tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga tcgttaaaga
    25381 tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag atctgaaggt
    25441 gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc gcctggttga
    25501 aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac tgaaatctga
    25561 gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc
    25621 atccggcaac gatgccgact gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc
    25681 atctgtataa atttcgaact acacttaact ggcttctctt aactgaggtc accatcatgc
    25741 cgttaagtcc ctacctctct tttgccggta actgttccga cgcgattgcc tattatcaac
    25801 gtacgttggg cgcggaactg ctctataaaa tcagcttcgg cgaaatgccg aaatcagcgc
    25861 aggacagcgc cgagaactgc ccttccggaa tgcaatttcc cgataccgcc atcgctcatg
    25921 ccaacgtgcg cattgccgga agcgacatca tgatgagcga tgctataccg tcaggaaaag
    25981 ccagctactc cggctttacg ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct
    26041 ggtttgataa ccttgccgct aacggaaaaa tcgaaatggc ctggcaggaa actttctggg
    26101 cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt aatgtcgtca
    26161 aacaacaacc aacgcaataa cccgccggga ggcccgccct cccgcactgt catcgaattc
    26221 ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa taatccgcca
    26281 cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt gtcgagaagc
    26341 tcgccaaaac cttcaatcag catcaagcgc tgcatgcggt tgatctgaac attcatcaca
    26401 gtgaaatggt ggctctgctt gggccgtcgg gttccggcaa atccaccctt ttacgtcatt
    26461 taagcggttt gattaccggc gataaatccg ccggtagcca tatcgagcta ctgggccgca
    26521 ctgtccagcg cgaaggccgc ctggcccgcg atatccgcaa aagccgcgcc aacaccggct
    26581 acatctttca acaattcaac ctggtgaacc gcctgagcgt actggagaac gtgctgattg
    26641 gcgcgctcgg cagcacgccg ttctggcgca cctgttttag ctggttcacc ggcgagcaga
    26701 aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcattttgcc catcagcgcg
    26761 tttccaccct ctccggcggc cagcagcaac gtgtggcgat tgcccgtgcg ctgatgcagc
    26821 aggcgaaagt gattctggcc gatgaaccca tcgcctcgct ggacccggaa tcagcgcgca
    26881 tcgtgatgga caccctgcgc gacatcaacc agaacgacgg catcaccgtg gtcgtcacgc
    26941 tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg cgtcaggggc
    27001 acgtcttcta cgacggcagc agccaactgt ttgataacga acgttttgac catctctacc
    27061 gcagcattaa ccgcatcgaa gagaacgcga aagctgcctg atatccccat cattgaggaa
    27121 aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc agcctcagca
    27181 ccctgttaac cccggcacac gccgaagagc aggaaaaggc gctgaatttc ggcattattt
    27241 caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gtttttacag gatatggaga
    27301 agaaactggg cgtgaaggtc aacgccttct ttgccccgga ctacgcgggc attatccagg
    27361 ggatgcgctt caataaagtg gatattgcct ggtacggcaa cctgtcggcg atggaagcgg
    27421 tggatcgcgc caatggccag gtcttcgccc agacggtcgc ggcggatgga tcgccgggtt
    27481 actggagcgt gttgatcgtc aacaaagaca gcccgatcaa caacctgaac gatctgctgg
    27541 cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg
    27601 tccccggtta ctacgtcttc gctaaaaaca atatctccgc cagcgacttc aagcgcaccg
    27661 tcaacgccgg gcatgaaacc aacgcgctgg ccgtcgccaa caagcaagtg gatgttgcca
    27721 ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag cttaaagagc
    27781 tgaaggtgat ctggaaatcg ccgctgatcc taggcgatcc gatcgtctgg cgcaagaatc
    27841 tgtccgaaac taccaaagac aagatctacg acttctttat gaactacgga aaaacgccgg
    27901 aagagaaagc ggtgctggaa cgcctgggct gggcgccgtt ccgcgcttcc agcgacctgc
    27961 aactggtgcc gattcgccag ctcgcactgt ttaaagagat acaaagcgtg aaaggcaata
    28021 aagggctgaa tgagcaggac aagctggcga aaaccaccga gattcaggcg cagctggatg
    28081 acctggaccg cctgaacaac gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt
    28141 aaatcgtagg tcggataaga cgccccggcg tcgcatccga caatgtgtag gcgttgatgc
    28201 cggatgcggt gcaagcacct tatccggcct acagaccgga gccaaacatg caaaccatca
    28261 ccatcgcccc acccaagcgt agctggttct cgcttctgag ctgggccgtt gttctcgacg
    28321 tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt aaagacggcg
    28381 gcaacatggc aacctt
//
LOCUS       DAJTPG010000047        28257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTPG010000047 DAJTPG010000000
VERSION     DAJTPG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO230_002117"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO230_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO230_002118"
     CDS             complement(1023..2045)
                     /locus_tag="OO230_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5691873.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO230_002119"
     CDS             complement(2059..3561)
                     /locus_tag="OO230_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5691874.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO230_002120"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO230_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO230_002121"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO230_002121"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5691875.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO230_002122"
     CDS             complement(5877..6218)
                     /locus_tag="OO230_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5691876.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO230_002123"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO230_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5691877.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO230_002124"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO230_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5691878.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO230_002125"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO230_002125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5691879.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO230_002126"
     CDS             12897..14240
                     /locus_tag="OO230_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5691880.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO230_002127"
     CDS             complement(14302..14508)
                     /locus_tag="OO230_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5691881.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO230_002128"
     CDS             14833..15387
                     /locus_tag="OO230_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5691882.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO230_002129"
     CDS             complement(15450..16388)
                     /locus_tag="OO230_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691883.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO230_002130"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO230_002130"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5691884.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO230_002131"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO230_002131"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5691885.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO230_002132"
     CDS             complement(19599..19979)
                     /locus_tag="OO230_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5691886.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO230_002133"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO230_002133"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5691887.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO230_002134"
     CDS             21037..22002
                     /locus_tag="OO230_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5691888.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="OO230_002135"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="OO230_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5691889.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO230_002136"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO230_002136"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5691890.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO230_002137"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO230_002137"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5691891.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="OO230_002138"
     CDS             25376..25921
                     /locus_tag="OO230_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5691892.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="OO230_002139"
     CDS             complement(25905..26552)
                     /locus_tag="OO230_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5691893.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="OO230_002140"
     CDS             26764..27555
                     /locus_tag="OO230_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5691894.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28257
                     /locus_tag="OO230_002141"
     CDS             27565..>28257
                     /locus_tag="OO230_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5691895.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDG"
BASE COUNT         6930 a         7530 c         6967 g         6830 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tataaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggc
//
LOCUS       DAJTPG010000048        28222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTPG010000048 DAJTPG010000000
VERSION     DAJTPG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1436)
                     /gene="yccS"
                     /locus_tag="OO230_002142"
     CDS             complement(<1..1436)
                     /gene="yccS"
                     /locus_tag="OO230_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5691896.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVL"
     gene            complement(1446..1892)
                     /locus_tag="OO230_002143"
     CDS             complement(1446..1892)
                     /locus_tag="OO230_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5691897.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2015..4069
                     /gene="helD"
                     /locus_tag="OO230_002144"
     CDS             2015..4069
                     /gene="helD"
                     /locus_tag="OO230_002144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5691898.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4101..4559)
                     /gene="mgsA"
                     /locus_tag="OO230_002145"
     CDS             complement(4101..4559)
                     /gene="mgsA"
                     /locus_tag="OO230_002145"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5691899.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(4655..5317)
                     /locus_tag="OO230_002146"
     CDS             complement(4655..5317)
                     /locus_tag="OO230_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5691900.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            5490..5903
                     /locus_tag="OO230_002147"
     CDS             5490..5903
                     /locus_tag="OO230_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5691901.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(5948..6265)
                     /gene="hspQ"
                     /locus_tag="OO230_002148"
     CDS             complement(5948..6265)
                     /gene="hspQ"
                     /locus_tag="OO230_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5691902.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6323..7513)
                     /gene="rlmI"
                     /locus_tag="OO230_002149"
     CDS             complement(6323..7513)
                     /gene="rlmI"
                     /locus_tag="OO230_002149"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5691903.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            7608..7886
                     /gene="yccX"
                     /locus_tag="OO230_002150"
     CDS             7608..7886
                     /gene="yccX"
                     /locus_tag="OO230_002150"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5691904.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(7883..8212)
                     /gene="tusE"
                     /locus_tag="OO230_002151"
     CDS             complement(7883..8212)
                     /gene="tusE"
                     /locus_tag="OO230_002151"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5691905.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8303..8962)
                     /gene="yccA"
                     /locus_tag="OO230_002152"
     CDS             complement(8303..8962)
                     /gene="yccA"
                     /locus_tag="OO230_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5691906.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9169..9256)
                     /locus_tag="OO230_002153"
     tRNA            complement(9169..9256)
                     /locus_tag="OO230_002153"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9220..9222),aa:Ser,seq:tga)
     gene            9682..10800
                     /gene="hyaA"
                     /locus_tag="OO230_002154"
     CDS             9682..10800
                     /gene="hyaA"
                     /locus_tag="OO230_002154"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5691907.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            10797..12590
                     /gene="hyaB"
                     /locus_tag="OO230_002155"
     CDS             10797..12590
                     /gene="hyaB"
                     /locus_tag="OO230_002155"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5691908.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            12609..13316
                     /gene="hyaC"
                     /locus_tag="OO230_002156"
     CDS             12609..13316
                     /gene="hyaC"
                     /locus_tag="OO230_002156"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5691909.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13313..13900
                     /locus_tag="OO230_002157"
     CDS             13313..13900
                     /locus_tag="OO230_002157"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5691910.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            13897..14295
                     /gene="hyaE"
                     /locus_tag="OO230_002158"
     CDS             13897..14295
                     /gene="hyaE"
                     /locus_tag="OO230_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5691911.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14292..15149
                     /locus_tag="OO230_002159"
     CDS             14292..15149
                     /locus_tag="OO230_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5691912.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15283..16827
                     /gene="appC"
                     /locus_tag="OO230_002160"
     CDS             15283..16827
                     /gene="appC"
                     /locus_tag="OO230_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5691913.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            16839..17975
                     /gene="appB"
                     /locus_tag="OO230_002161"
     CDS             16839..17975
                     /gene="appB"
                     /locus_tag="OO230_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5691914.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            17987..18079
                     /gene="cbdX"
                     /locus_tag="OO230_002162"
     CDS             17987..18079
                     /gene="cbdX"
                     /locus_tag="OO230_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5691915.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18159..19457
                     /gene="appA"
                     /locus_tag="OO230_002163"
     CDS             18159..19457
                     /gene="appA"
                     /locus_tag="OO230_002163"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5691916.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(19572..21738)
                     /gene="etk"
                     /locus_tag="OO230_002164"
                     /pseudo
     CDS             complement(19572..21738)
                     /gene="etk"
                     /locus_tag="OO230_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(21758..22204)
                     /gene="etp"
                     /locus_tag="OO230_002165"
                     /gene_synonym="yccY"
     CDS             complement(21758..22204)
                     /gene="etp"
                     /locus_tag="OO230_002165"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5691917.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22192..23331)
                     /locus_tag="OO230_002166"
     CDS             complement(22192..23331)
                     /locus_tag="OO230_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5691918.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(23377..25473)
                     /locus_tag="OO230_002167"
     CDS             complement(23377..25473)
                     /locus_tag="OO230_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5691919.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(25473..26219)
                     /locus_tag="OO230_002168"
     CDS             complement(25473..26219)
                     /locus_tag="OO230_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5691920.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26216..26860)
                     /locus_tag="OO230_002169"
     CDS             complement(26216..26860)
                     /locus_tag="OO230_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5691921.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(26967..27272)
                     /locus_tag="OO230_002170"
     CDS             complement(26967..27272)
                     /locus_tag="OO230_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691922.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(27714..27926)
                     /gene="cspH"
                     /locus_tag="OO230_002171"
     CDS             complement(27714..27926)
                     /gene="cspH"
                     /locus_tag="OO230_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5691923.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6824 a         7146 c         7423 g         6829 t
ORIGIN      
        1 aagagcacgc cggtaataac cagcagcacc agctgccctt ccagtgatgg cacaaaccac
       61 agcacaggaa tgccaatcgc gatacctacc agcgtaccaa taatccttaa cttcaggcgg
      121 tggcgcgtgg cgttatagtt tggctggcag acaaacaaac ttgtcagcaa gatccaatac
      181 ccgtgatgca ttccggttat ttgaatgatg gcgtagccga agcacaacac cagcgacatt
      241 cttaccgcat gacggaagag ggcggattcc ggcgtgaagt gacggctaag acgcagccag
      301 atatcactca atccgtgcgg gctgtcatca gcgagctcat tttcgtcatt attacggggt
      361 agtgcctggg cctgttctga ttcaattgtt gccagttggg catcaatggc gcgtaaattg
      421 ttcagcaaaa atcccagtgt tttgagtaaa tcggcgggtg cgccgttatc gcgtatccgc
      481 tccagcgcag catcaatatg cgtaaaagcg cgctcaaaat gcggatcatg ttgataagac
      541 tgacgcaaca aaatacagcg tgacagttgc tggcacgcct ggccctgcat cgacatcagc
      601 cgctgaaaac ggaacagcac gtcgctgtgg cgaaaatgtt cacgcaatgt ttgatactga
      661 atatgagaag agctggcacg ctcgtgaata tcctgtgcga caaagtaata atgcagcgtg
      721 cgacgcgttc cccgttgacc acgatcgcca cgtaagcggg tcagcagcga gagtttcgtc
      781 tgattcaatg tcgccatcag ctgaccgttg gcgagagcca aatcgtacag cggtgcctgg
      841 ctttcatctt caatatcagg atcaaacatg cgcgacttga gctcaagata acgcgccagt
      901 tgttcatagc aacgcgccag gttgtcctgc agcgggcgga ccgggaacag cagatgacca
      961 ataagtgtca ggacgttgta ccagacggca ccggccagca gatacatcgg ctgctgatac
     1021 cagtgctcat acagtgatgt tcccaacata gtgtaaatgg cgatcagcaa tgcaccgaag
     1081 gcaattgttg catagcgttg acccagaccg ccgagcaaaa tgaagccgct ggtagagagc
     1141 gttaagccaa tcgcaaatag ccagggccag ggaaacagca attctactga ggccgaggcg
     1201 ataaaaaagc agaacagcgt aatgatgagg ttacgcaaac gtcccgccag tcggtcatcg
     1261 agatcggtca gcgccgctgc caccatcccc agcgttagcg gaatcgtcag ttttacatca
     1321 cccagccacc acggaaacgc tgtggttcca caaagcgcaa taaaaatacg cgcgtaatac
     1381 agccaggcgc tgttccaggt atagcgtttg agcaaaggac ttagcataaa ggccatatta
     1441 gtggattatt caaaacgacg acgtgcattg gcttcacgcg cagcttgcgc tgtttctacc
     1501 gataccacgc gacgaccaac cggccatagt gcaatagcgg caattttaaa gttcgcaatg
     1561 ccgacaggaa tgccaatgat tgaaatacat tgtgcgatgc ccgttgcaat gtgcatcagg
     1621 cataaccacc agccaaagaa aatcagccag aaaatattca ataccgtacc gccagtattc
     1681 agcaacacat ttttgccagc cgggttcagt tcatcgacat gaatagcttc attgccataa
     1741 ggcaccagag acagtttagt gatctcccag caggatcgtg tcagcggtaa ggtaaaaatc
     1801 agcacaatac tgaccagagt cgccaacagc cagcccagag tggtggcaaa tccgccaagc
     1861 acaaagttca gaatgttcaa aacggtacgc ataaaacctc gctttactgt ggttttcagt
     1921 aatgggcggc aattgtaacg tttttttggg ctggagcacg ttttctctga cggttacact
     1981 gataagaaat aatttcgtgt ggatctacag agtcatggaa ctgaaagcga caacgcttgg
     2041 aaaacgtctg gcacagcacc cttacgatcg ggcggtgatc ctcaatgccg ggattaaagt
     2101 ctccggcgat cgccacgaat accttattcc tttcaatcaa ttactggcga ttcactgtaa
     2161 gcgcggtctg gtatggggcg agctggaatt tgtactgccg gacgaaaaag tggtgcgtct
     2221 gcacggcaca gaatggggcg agacgcagcg tttttaccat catcttgatg ctcactggcg
     2281 gcggtggagt ggcgagatga gcgaaattgc gtctggtgtt ttacgccagc aactggattt
     2341 gattgccacg cgcactggag aaaataaatg gctgacgcgt gagcaaacct ctggcgttca
     2401 gcaacaaatc cgccaggctt tgtcggcgtt gccgttgccg gttaaccgac tggaagaatt
     2461 cgataactgc cgtgaggcgt ggcgtaaatg tcaggcttgg ttgaaagata ttgaaagcgc
     2521 tcggttgcag cataaccagg cgtataccga agccatgctt accgagtatg cggatttttt
     2581 ccgccaggtc gagtcttcac cgctgaatcc ggcgcaggcc cgggcagtcg ttaatggcga
     2641 gcattctctg ttagtgctgg ctggtgcagg aagcggaaaa acgtcggtgc tggtggcccg
     2701 tgcaggctgg ttgctggcgc gtggtgaagc gtcccctgag caaattttat tgctggcgtt
     2761 tggtcgcaaa gccgctgaag agatggacga gcggattcgc gaacggctac ataccgaaga
     2821 cattaccgca cgcacgtttc atgcgttggc gctgcatatt attcagcagg gcagcaaaaa
     2881 agttccgata gtcagcaaac tggaaaatga taccgctgcc cgtcatgaac tctttattgc
     2941 tgagtggcgc aagcaatgca gcgaaaagaa agcgcaggcg aagggctggc ggcaatggct
     3001 gacggaagaa atgcagtggt cagtgccaga aggtaacttc tgggatgatg aaaaattaca
     3061 gcgtcgcctt gcctctcgcc tcgatcgctg ggtaagtctg atgcggatgc acggtggtgc
     3121 acaggcagaa atgattgcca gtgcacccga agagattcgc gatctgttca gtaaacgtat
     3181 caagttgatg gccccgttat taaaagcctg gaaaggtgcg ctgaaggcag aaaacgctgt
     3241 cgatttttcg ggccttattc atcaggcgat tgtgattctg gagaaaggcc gctttatcag
     3301 cccgtggaag catattctgg ttgatgaatt tcaggatatc tcgccgcagc gcgcagcgtt
     3361 gttagcggca ttacgcaagc aaaacagtca gacgacgttg ttcgccgttg gtgatgactg
     3421 gcaggcgatt taccgattca gcggtgcgca aatgtcgctc accaccgctt tccatgaaaa
     3481 ctttggtgaa ggcgatcgct gtgatttaga cacgacttac cgttttaaca gtcgtatcgg
     3541 tgaggtggca aaccggttta ttcagcagaa cccaggccag ctgaaaaagc cgctaaacag
     3601 cttaaccaat ggagacaaaa aagccgtcac gttattggat gagagtcaac ttgacgcttt
     3661 gctggataag ctctctggtt atgccaaacc ggaagagcgc attctgatcc tggcgcgtta
     3721 ccatcacatg aggcctgcca gcctggaaaa agcggcaaca cgctggccga agttgcaaat
     3781 cgactttatg accattcatg ccagcaaagg gcaacaggcg gattacgtca tcatcgttgg
     3841 cttgcaggag gggagtggtg gttttccggc tgcggcgcgg gagtcgatta tggaagaggc
     3901 gctactgcca ccggttgagg atttcccgga cgctgaagaa cggcgtttaa tgtacgtggc
     3961 gctgacccgg gcacgccatc gggtatgggc actgtttaac aaagagaatc cctctccctt
     4021 tgtggaaata ctgaaaaatc tggatgtgcc ggtggcgaga aaaccgtaag aaacaggtgg
     4081 cgtttgccac ctgtgcaata ttacttcaga cggtcagcga gataacgctg ataatcgggg
     4141 ataagaatat cgaccgcgtc gttgaaatgc ggcgactgga taatgaagtc tgccgttgcc
     4201 acgttggtgg cgaccggaat gttccatacc gtcgccagac gcagcaaggc tttcacgtca
     4261 ggatcgtgcg gcacggcatt tagtggatcc cagaagaata tcaatacatc aattttccct
     4321 tctgagatca atgcgccaac ctgctggtca ccccccattg ggccactcaa catcgcgttg
     4381 acgttcatgc cggtcgcgcg ggaaatcaag ttaccggtag tgcctgttgc atacagtacg
     4441 tgttgttcca gtaacggttg atgccgttcc acccagctca tcagcatttg tttgcagtga
     4501 tcgtgtgcca ccagcgcaat atgtttccgc gcaggtaaag tgcgagtcgt cagttccata
     4561 atgtacatcc gtagttaact ttcctacaga ttactgtaag cacttatcgc tgcaagataa
     4621 agaccgaaaa agcctgcgca caggcacaaa aatctcagga agatggttgt ttttccgccc
     4681 attgcaggaa agtatttcgc gtttgtgggt cagccagttt aaaccaatac ttcagccgtt
     4741 gttctgtgag cacctgagac tgcggtggaa tagcatccag ctcggaaaca cccgaaagca
     4801 gcgtactgtc atctgccatc atcgtggcaa attgcggtag tgaagcgcgt ttcgcggatt
     4861 tgttatagcg ttcaacctcg acctcataat caatcgtttt tgccgttgag gtaatggcga
     4921 gaatatccag ctttaccgga atcggcgtcg catcgccatc caacaattca aggcgcggcg
     4981 cggcatcaaa atggttagct tcccgctcat tctccaggcg aggcaggcga aaattgacct
     5041 ggttgatgag ctgggtatta aaactgacca ccagcggtgg ggagatatac agccgttctt
     5101 cactattgga aagatgaatc gtcttctcaa cgcgaaacac taactgatgc ggtccattgt
     5161 ccagttcaat gctgtccgca ccgcgcagca gagagctgga aaccttcttg ccgtcgagta
     5221 ccagaagatc gacatcggtt gaaagccgca gcgtggtggc aaaaacggat accggcaaac
     5281 ataatgcaat caaggtggtc acgatgccgg ttttcatagg tagctcctgt caaaagaccg
     5341 cccgaataat aatgtgtcaa aatttttctt taatcatact tactaacata tagacatatt
     5401 tcccgctttt gctctcattc attcgtatta gctgcatggt tggcatgtcg gctttgtcgt
     5461 acactttgtt aaacagtctg gaggaaataa tgaaagaaac cgatattgca ggcattttaa
     5521 cgtccactca caccattgcg ctggtggggg caagcgacaa acccgatcgc cccagctatc
     5581 gcgtgatgaa atatctactc gaccaggggt atcacgtcat tccggtttcg ccaaaagttg
     5641 ccggcaaaac gctgctgggc cagcaggggt atggcacgct ggcagacgtt ccggaaaagg
     5701 tcgatatggt cgatgttttc cgtaattcag aagcggcgtg gggcgtggca caggaagcta
     5761 ttgccattgg cgcgaaaacc ctctggatgc aactgggggt gattaatgag caagcggcag
     5821 tactggcacg ggatgccggg ttaaatgtgg tgatggatcg ctgcccggct attgagatcc
     5881 ctcgcctggg tctggccaaa taaaaaatcc ccggaaggca aaaaccttcc ggggatttgt
     5941 tcagggatta gttacgcaga cgcggggcct ggagttgttt gcggatggtc tgcgccagct
     6001 catccatgga tggctgttca ggatgttcat cctgcaattc gctacttagc tgggcttcag
     6061 ccaggtaggt atgaaccggt aggccgttat cgtcctccat caccacgtga taccacggag
     6121 cggcgcgaag ctcgtcattc accgccagct catcgggcga cggttcagag agcgaataaa
     6181 ccgggtcgat atccacgacc actccgagat aacctaacag ggaatggcgg acctgctggc
     6241 cgataccgaa tttgctggca atcatagtca cctcccggga aatctttacc tttgatataa
     6301 gggtaaaatt ccacatttca agttacatga cgcgacaggc aaaccctttc agatatagcc
     6361 cttccgggta ggtagcgatc accggatgat cggctgcctg acggaactgc tctataaatt
     6421 gtacatcacg accggcatca attgcggcat ccgcgatgat tttctgaaat aaatcgctgg
     6481 tcatcagacc ggaacaggag aaagtcagga gaataccgcc ttcattcagc aactgaatcg
     6541 ccagcatgtt gatgtcttta taaccacgac acgcgcccat caactggctt ttattctcaa
     6601 caaacttcgg cgggtccatc acgataacgt caaatttttc accgcgatcg cgataagtac
     6661 gcagcaattt aaagacatca tcacggacaa actcagcctt gctcagatcc agtttgttca
     6721 gctcaacgtt ctgccgtgca atatccagcg cttcctggga ggtatcaacg ctgacaacct
     6781 ggctgcaacc gcccatcagt gccgatacgg cgaaaccacc ggtataggag aaacagttca
     6841 gcacacgttt attttcaacg tagcggcggg tagccaggcg gctgtctcgc tggtccagat
     6901 agtagcccgt tttgtgtccg tgctgaatat ccaccagcag cttcatgccg tgttcttcaa
     6961 tcggcagcag ggcaggcggc aactcgccgg tgacgaggcc ctgggtcagc tccatccctt
     7021 cttttttacg taccgcaaca tcgctgcgat cgtaaatcgc acattccggg tacagcgttt
     7081 gcagggcact aattaatgcc gcgcgctgat attctgcgcc agcactcagc agttgcagca
     7141 ccagaaaatt accgaaacga tcgatagtaa tacccggcag gccatcagat tctccggcga
     7201 ttaaacgata gctgtcgagg ccatcttttt gcgccagcca gtcacgccat ttttgtgctt
     7261 gttgcaaacg gcgggaaaaa aaagcaatgt cgatagactc agacgggtca aacgtccaga
     7321 cgcgcgcccg gatttgcgaa gctggcgaat aagcgccgcg tgctaaccat tttccctgat
     7381 gatcaacaat atcgatggtt tcaccgaggc tggctttacc ttccatgcgg gcaacggccc
     7441 cggaaaagac ccacggatgg cgacgaagta atgatttttc gcgccctttg gctaacacta
     7501 aacgtacact cataatttac ttttctgtcg atgcaaagaa atgggcgtca ttgtccggag
     7561 ttttccatgg atttgcaacg cgtccattaa ggataaggag agcagcaatg tcgaaaatct
     7621 gcataattgc ctggatttac gggcgagttc agggcgtagg atttcgctac accacacagt
     7681 acgaagcgaa aagactgggc ttaaccgggt acgccaaaaa tcttgacgat ggcagcgtgg
     7741 aagtggttgc ctgcggtgaa gaagggcagg tagaaaaatt aatgcagtgg ttaaagagtg
     7801 gcggcccgcg ttctgcgcgt gtagaacggg tgctcagcga gccgcatcat ccctcggggg
     7861 aattaaccga ttttcgtatc cgttaaatac attttaccgg ttttggcagg ccagcaattt
     7921 tggtggcttg cttagccggg cctttcggga acagtcggta taaatagcgg ctgttacctt
     7981 tttcttcacc aaatttattc gccatcgctt ttaccagcat acgaatcgcc ggagacgtat
     8041 taaattccag gtagaagtca cgtacaaaac gcaccacttc ccagtgttct ggcgacagcg
     8101 aaatcccttc gttctctgca atcaccactg ccagcggttc actccactgg ctgctttctt
     8161 tgagatagcc ttcggtatcc gtttctatct ctttaccttc gaagatcagc ataattatta
     8221 ctaccttaat cagactggcg gcagtgtaac aaaaaacaaa gccccgcata aagcgaggct
     8281 atgaaagtgt tagcgggtga gattaatcgc ggctggcgaa gcccagaatg ctcagtaggc
     8341 tgacgaagat gttgtacagc gaaacataca ggctaaccgt ggcacgaata tagttcgttt
     8401 caccgccatg aatgatgttg ctggtttcaa acaagatagc gccagaggag atcagaatga
     8461 agaccgcgct gatcgccaga tgcagagcag gcagttgcag gaagatatta gcaatcatac
     8521 caatcagtac caccacaata cccgccatca gcataccgcc gaggaacgac atatctttgc
     8581 gggtggtcag cacatatgca gagcagcaga agaacactaa cgccgttccg cccagtgcca
     8641 tagcgattac gtcacccatt ccggcagaca gataggtgtt cagaattggt ccgaggatat
     8701 aacccagaaa accggtaaag gcgaatgcgg agataatccc ggtcggctta ttcgccgttt
     8761 tataggtcag gaacatcaaa ccatacatac ccaccagagt cagaatcaga cccggagatg
     8821 gcagcatcag caccgtactg gcagttgcgg taatcgccga aaaggccagc gtcaggctca
     8881 gcagaaaata ggtattacgc agcaccttat gggtgctaag cagtgatgta cggtcatgtg
     8941 aagaactaac aatacgatcc attgagtcac tctctatgac agatgtaatt aattaagcag
     9001 cataatgata atgcgtaagg gcacccagaa gttttaccca tctttacgca tttgatctgg
     9061 aacaggttta acagcggatt atcaggtcat taagcaaata taacgccctg agaattccga
     9121 caggcaaaag aaaaaggggg tagcgtttag ctaacccctt atcttatttg gcggaagcgc
     9181 agagattcga actctggaac cctttcgggt cgccggtttt caagaccggt gccttcaacc
     9241 gctcggccac acttccggaa tgacgcgcac tataaacatc ccgatgcggc gtgtaaaccc
     9301 ctaatttgtt tgtttgcctg aaaaacagcc aaaagtgcat tgatagcgtg aaataacagc
     9361 agattgatca tttcatcacc atgaattcct tctcttttac tcgtttagca accggctaaa
     9421 catccccatc gcccggctaa agaaaaatag gtccattttt atcgctaaaa gacaaatcca
     9481 cacagtttgt attgttttgt gcaaaagttt cactacgctt tattaacaat actttctggc
     9541 gacgtgtgcc agtgcagaag gatgagcttt cgttttcagc atctcacgtg aagcgatggt
     9601 ttgccttgct acagggacgt cgcttgccga ccataagcgc ccggtgtcct gccggtgttg
     9661 ctaggagaag agacgtgcga tatgaataac gaggaaacat tttaccaggc catgcggcgt
     9721 cagggcgtta cccggcgcag ctttctcaaa tattgtagtc tggctgccac gtcgctggga
     9781 ttaggcgcgg gaatggcacc aaagattgcc tgggcgctgg agaacaaacc gcgcattccg
     9841 gtggtatgga tccacggtct ggaatgtacc tgctgtaccg aatcttttat ccgctccgct
     9901 cacccactgg cgaaggacgt catcctttcc ctgatttccc tcgattacga cgataccctg
     9961 atggctgccg ccggaaccca ggcggaagaa gtctttgaag acatcatcac gcaatataat
    10021 ggcaaatata tcctcgcagt agaaggtaat ccgccgctgg gcgagcaggg gatgttctgt
    10081 atcagcagcg gtcgaccgtt tattgagaaa ctcaaacgtg ccgctgccgg agccagcgcg
    10141 attatcgcct ggggaacctg cgcgtcctgg ggctgcgtgc aggccgcgcg acctaatccg
    10201 acacaggcaa cgcctatcga caaagtcatc accgacaaac ccattatcaa agtacctggc
    10261 tgcccgccga tcccggatgt gatgagcgcc atcattactt acatggtgac ctttgatcgc
    10321 ttgccagatg tcgacagaat gggtcgtcca ctgatgtttt atggtcagcg tatccacgat
    10381 aaatgctatc gccgcgctca cttcgacgcc ggagagttcg tccagagttg ggatgatgac
    10441 gctgcccgca aaggttattg cctgtacaaa atgggctgca aagggcctac cacctataac
    10501 gcctgttcct ccacacgctg gaatgatggc gtttctttcc caatccagtc tggtcacggc
    10561 tgcctgggct gtgcggaaaa tggtttctgg gatcgcggtt cgttctacag ccgcgtggtc
    10621 gatattccgc aaatgggtac tcattccacc gccgataccg tcggtttaac cgcgcttggc
    10681 gtggtggcag cagctgttgg tgtgcacgca gttgccagcg ccgttgacca acgcagacgt
    10741 cataaccagc aacctacaga aaccgaacat cagccaggca atgaggataa acaggcatga
    10801 gcactcagta cgaaactcag ggatacacca tcaataatgc cggacgccgc ctggtggtcg
    10861 acccgattac gcgcatcgaa ggccacatgc gctgtgaagt gaatattaac gatcagaatg
    10921 tgatcaccaa tgccgtctcc tgcggcacca tgtttcgcgg gctggagatc atcctgcaag
    10981 ggcgcgaccc gcgcgatgcg tgggcgttcg ttgaacgtat ctgcggcgtc tgtactggcg
    11041 tacacgccct ggcttcggtt tacgccatcg aagatgccat cggtattaaa gtgccggaca
    11101 acgccaatat catccgcaac attatgctgg caacgctctg gtgccacgat catctggtgc
    11161 acttctatca gcttgccggg atggactgga tcgacgtgtt agatgcgctg aaagccgacc
    11221 cgcgaaaaac ctccgaactg gcgcaaagtc tctcctcttg gccgaaatca tcccctggct
    11281 atttcttcga cgtacaaaat cgcctgaaga aattcgttga aggcgggcag ttggggatct
    11341 tccgcaatgg ctactggggg cacccgcagt acaaactgcc gccagaagcc aacctgatgg
    11401 gctttgccca ctatctcgaa gctctcgatt tccaacgtga aattgtcaaa attcacgcgg
    11461 tctttggcgg taaaaacccg catccaaact ggattgtcgg tgggatgcct tgcgccatta
    11521 acattgacga aagcggcgcg gtcggggcgg tcaatatgga acgcctgaac ctggtgcagt
    11581 cgattatcac ccgcacggca gacttcatta acaacgtgat gatccccgac gccttagcca
    11641 tcggtcagtt caacaaaccg tggagcgaaa tcggcacggg tctttctgat aaatgtgtcc
    11701 tcagctacgg cgcattcccg gatattgcca acgactttgg tgagaaaagt ctgctgatgc
    11761 ctggcggcgc ggtgattaac ggcgacttca acaatgtgct gccagtggat ttggttgatc
    11821 cgcagcaggt gcaggagttt gtcgatcacg cctggtatcg ttatcccaac gatcaggtcg
    11881 ggcgtcatcc attcgatggc attaccgacc cgtggtacaa ccccggcgat gtcaaaggca
    11941 gcgataccaa cattcagcag ctgaatgaac aggaacgcta ctcgtggatc aaagcgccac
    12001 gctggcgcgg taacgcgatg gaagtggggc cgctggcacg cacgttaatc gcttatcaca
    12061 aaggcgatgc tgcgaccgtt gagtcggtcg atcgcatgat gtcggcgttg aacctgccgc
    12121 tttccggtat ccagtcaacg ttgggccgca ttttgtgccg cgcgcacgaa gcgcagtggg
    12181 ccgcaggtaa gttgcagtat ttcttcgaca agctgatgac caacctgaaa aacggcaatc
    12241 tcgccactgc ttccactgaa aaatgggaac ctgcaacctg gccgacagag tgccgtggcg
    12301 tcggctttac cgaagcgccg cgcggagcgt taggccactg ggccgccatt cgcgatggca
    12361 agattgatct ctaccagtgc gtggtgccga ccacctggaa cgccagtccg cgcgatccca
    12421 aagggcagat tggcgcttat gaagcggcgc tgatgaacac caaaatggcg atccccgagc
    12481 aaccgctgga gatactgcgt actctgcaca gctttgatcc gtgcctcgcc tgttcaactc
    12541 atgtgctggg cgacgacggt agcgagctga tctccgtgca ggtgcgttaa cagcgaagga
    12601 gaatcatcat gcaacagaaa agcgacaacg ttgtcagcca ctatgtcttt gaagcaccag
    12661 ttcgtatctg gcactggctg acggtgttat gcatggcggt gttgatggtc accggttact
    12721 ttatcggcaa gccgctacct tccgtcagtg gcgaggcgac gtatctgttc tatatgggtt
    12781 acatcaggtt aattcacttc agcgccggga tgatttttac cgtggtcttg ctgatgcgga
    12841 tctactgggc ttttgttggc aatcgttact cccgcgagct gtttatcgtg ccggtatggc
    12901 gtaaaagctg gtggcagggc gtgtggtatg aaatccgctg gtatctgttt ctggcaaaac
    12961 gtccgagtgc cgatataggc cataacccca tcgcccaggc ggcgatgttc ggctatttcc
    13021 tgatgtcggt ctttatgatc atcaccggtt ttgcgctgta cagcgaacac agccagtacg
    13081 ctatttttgc gccgttccgt tatgtggtgg aatttttcta ctggacgggt ggcaactcaa
    13141 tggacattca cagctggcat cggctgggga tgtggctgat tggggcgttt gtgatcggtc
    13201 atgtctacat ggcgctgcgt gaagacatca tgtccgacga cacggtgatc tccaccatgg
    13261 tcaacggcta ccgtagccac aaatttggca aaataagtaa caaggagcgt tcatgagcga
    13321 gcaacacgtg gtggtcatgg ggctgggcaa cctgctgtgg gccgatgaag gcttcggcgt
    13381 gcgggtggcg gaacggctgt atgcccatta ccactggccc gagtatgtgg agattgtcga
    13441 tggcggtact cagggactga acttgctggg gtatgtcgaa agcgccagcc atctgttgat
    13501 tctcgatgcc attgactacg ggctggaacc tggaacgctg cgaacctatg ccggagaacg
    13561 cattccggcc tatctcagcg cgaagaaaat gagcctgcat cagaacagtt tctccgaagt
    13621 gctggcgctg gcagatatcc gcggacatct gccagcacat attgccctcg tcgggctgca
    13681 acccgcgatg ctcgacgact acggcggcag cctgagcgaa ctggcacggg agcaactgcc
    13741 cgctgcggaa caatcggcgc tggcgcagtt ggctgcgtgg ggaagtgtgc cgcaaccggc
    13801 taatgaatcg cgttgtctca attatgactg tctgtcgatg gaaaattacg aaggcgttcg
    13861 cttgcgccag taccggatga cacaggagga gcagggatga gcaacgacac gccatttgat
    13921 gcgttgtggc aacgaatgct ggcgcgcggc tggacgccgg tcagtgaatc ccgtcttgac
    13981 gactggctta cgcaagcgcc agacggcgtg gtgttattaa gcagtgaccc gaaacgcacg
    14041 ccagaggtca gcgataatcc ggtaatgatt ggcgaattac tgcacgagtt tcccgactat
    14101 acatggcagg tggcgattgc tgaccttgag cagagcgaag ccatcggcga tcgctttggc
    14161 gcctttcgct ttcctgccac tttagtgttt accggcggaa actatcgcgg cgtgctgaat
    14221 ggtattcacc cgtgggcgga actgataaac ctgatgcgcg ggcttgtcga accgcagcag
    14281 gagcgtgcct catgagcgaa acttttttcc atctgctggg gccaggaacg caaccgaacg
    14341 atgacagttt cagcatgaat ccactgccga tcacctgtca ggtgaatgat gaaccgagta
    14401 tggcggcctt ggagcaatgt gctcacagcc cgcaggtgat tgcgctgtta aacgagttac
    14461 aacatcaact aagcgaacgc cagccgccgt tgggcgaggt gctggcagtc gatctgttaa
    14521 atctcaacgc cgacgatcgt cactttatca atacgcttct cggggaaggg gaagtgtcag
    14581 tgcgcattca gcaggctgac gacagtaaaa gtgaaataca ggaggcgatc ttctgcggat
    14641 tatggcgggt gcgcagacgt cgcggcgaaa agttgctgga ggacaaactg gaggctggct
    14701 gcgcgccgct ggcgttgtgg caggcggcaa cgcaaaatct cttgccgaca gattcgctgt
    14761 taccgccgcc cattgatggc ctgatgaatg gcctaccgtt ggcgcatgag ttactggcac
    14821 atgtacgtaa ccccgacgcg cagccgcaca gcattaatct gacgcaatta cccatcagcg
    14881 aggctgatcg gctttttctc tcgcgtctct gtgggccggg aaatattcag attcgtacca
    14941 ttggctatgg cgagagctat atcaacgcca cggggttacg ccatgtctgg catttacgct
    15001 gtacggacac cttaaaaggc ccgttactgg aaagttatga aatctgccca ataccggaag
    15061 tggtgctggc agcgccagaa gatttggtcg actctgcgca gcggcttagc gaggtatgtc
    15121 agtggctggc ggaagctgca ccgacgtaaa aagacggtaa gtatcgcttt cagtcttatg
    15181 aatatcgcaa tcggcgaata cctctggtcg tagagtttca ggataaagag ggagatctaa
    15241 cattatcggg ttatttttct ctcttcgcct acaggagtgc gcatgtggga tgtcattgat
    15301 ttatcgcgct ggcagtttgc tctgaccgcg ctgtatcact ttttatttgt accccttacc
    15361 ctggggctga tttttttgct ggctatcatg gaaaccattt acgtggttac cggcaaaaca
    15421 atctaccgcg atatgacgcg cttctggggt aagctcttcg gtatcaattt tgctcttggc
    15481 gtggctaccg gcctgaccat ggagtttcag tttggtacta actggtcatt ctattccaac
    15541 tatgtgggcg atatttttgg cgcaccactg gcgatggaag cattaatggc cttcttcctc
    15601 gaatccacct ttgtcgggct gttcttcttc ggctggcaac ggctgaataa ataccagcac
    15661 ctgctggtga cgtggctggt ggcgttcggt tcaaatctct ctgcattgtg gatattgaat
    15721 gccaacggtt ggatgcaata cccgaccggt gcgcattttg atatcgacac cctgcgtatg
    15781 gagatgacca gcttcagcga gctggtcttt aacccggtca gccaggtgaa atttgtgcac
    15841 accgtcatgg cgggctacgt gaccggggcc atgtttatta tggcgatcag cgcctggtat
    15901 ttactgcgcg gacgggagcg cgatgtcgca ttacgctcgt ttgccatcgg ttccgtcttc
    15961 ggtactctgg cgattatcgg taccctgcaa ctcggagaca gttctgcgta tgaagtcgcg
    16021 caagtacaac cggtaaaact ggcggcgatg gaaggggagt ggcaaacgga acctgctcct
    16081 gcaccgttcc atgtggttgc ctggccggaa caggatcaag agcgtaacgc ctttgccatc
    16141 aaaattcccg cgctgctagg gatcctcgcc actcactcat tagataaacc cgtgccgggt
    16201 ctgaagaatt tgatggctga aacctaccca cgcttgcaac gcggacgtat ggcctggctg
    16261 ttaatgcagg aaatatctca aggcaatcgt gagccgcatg tgttgcaggc attccgggag
    16321 ctggaaggcg accttggtta cggcatgttg ctctcccgct atgcgccgga tatgaatcat
    16381 gtcacagccg cacagtacca ggcggcgatg cgtggcgcga tacctcaggt tgcgccggta
    16441 ttctggagtt tccgcatcat ggtgggctgt ggttccctgc tgctactggt gatgctgatt
    16501 gcgcttgtcc agacgctgcg tggcaaaatc gaccagcatc gctgggtgct gaaaatggcg
    16561 ctctggagtt tgccgttgcc gtggattgcg attgaagccg ggtggtttat gaccgagttt
    16621 ggtcgtcagc cgtgggcgat acaggacatc ttaccgacat actccgcgca ctccgcttta
    16681 accacaggac aactggcttt ctcactgatc atgatcgtag ggctttacac cctgttctta
    16741 atcgccgaag tctacctgat gcagaaatat gcccgtctgg ggccgagcgc gatgcagagt
    16801 gaacaaccga cgcagcaaca ggggtaaagg agaaaatcat gtttgattat gaaacattgc
    16861 gcttcatctg gtggctgctg attggcgtga tcctggtggt ctttatgatc tccgacggat
    16921 ttgacatggg gatcggctgt ttgctgccgc tggtggcgcg taatgatgat gaacgccgga
    16981 tagtgattaa tagcgttggt gcgcactggg aaggcaatca ggtctggttg atcctcgcag
    17041 gtggggcatt atttgcagcc tggcccagag tgtatgcagc ggcgttttcc ggcttttatg
    17101 tggcgatgat cctggtgctg tgctcgctgt tcttccgtcc gctggccttt gattatcgcg
    17161 gaaaaattgc cgatgcacgc tggcgtaaaa tgtgggatgc cggtctggtc atcggcagtc
    17221 tggtgccgcc ggtagtcttc ggtatcgcct tcggcaactt gttgctcggc gtgccgtttg
    17281 ccttcacacc gcaattacgc gtggagtatc tcggcagctt ctggcaactg ctgacgccat
    17341 tccctttatt gtgtggtttg ctcagccttg ggatggtgat tttgcaaggt ggcgtctggt
    17401 tacaactgaa aactgttggt gtgattcatc tgcgttcaca gctggcgact aaacgcgctg
    17461 cactgttggt gatgctgtgc tttttgctgg caggttactg gctgtgggtc ggtattgatg
    17521 gctttgtact gctcgcccag gatgctaacg gtccttccca tccgttaatg aaactggtgg
    17581 cagtgctacc tggtgcctgg atgaataatt tcgtcgaatc gcccgttttg tggatcttcc
    17641 cgctgctggg gttcttctgc ccattgctga cggtgatggc gatttatcgt ggtcgcccgg
    17701 gttggggatt tttgatggca tcattgatgc aatttggcgt gattttcacg gcaggcatca
    17761 cgctgttccc ctttgtcatg ccgtcaagtg tgagtccaat ctccagcctg acgttgtggg
    17821 acagcacttc cagtcagctg acgctgagca ttatgttggt aatcgtgctg atatttttgc
    17881 ccattgtgtt gctctacact ctctggagct actacaaaat gtggggacgc atgacaacag
    17941 aaactctccg ccgtaacaaa aacgagttgt actaaggagc aggaacatgt ggtatttact
    18001 ttggttcgtc ggcattttgt tgatgtgttc gctctccacc cttgtgttgg tatggctgga
    18061 cccgcgtctg aaaagttaac gaacgtaggc ctgatgcggc gcattagcat cgcatcaggc
    18121 aatcaataat gttagatatg aaaagcggaa acatatcgat gaaagcgatc ttaatcccat
    18181 ttttatctct tctgattccg ttaaccccgc aatctgcatt cgctcagagt gagccggagc
    18241 tgaagctgga aagtatggtg attgtcagtc gtcatggtgt gcgtgctcca accaaggcca
    18301 cgcaactgat gcaggatgtc accccagacg catggccaac ctggccggta aaactgggtt
    18361 ggctgacacc gcgcggtggt gagctaatcg cctatctcgg acattaccaa cgccagcgtc
    18421 tggtagccga cggattgctg gcgaaaaagg gctgcccgca gtctgctcag gtcgcgatta
    18481 ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc cttcgccgcc gggctggcac
    18541 ctgactgtgc aataaccgta catacccagg cagatacgtc cagtcccgat ccgttattta
    18601 atcctctaaa aactggtgtt tgccaactgg ataacgcgaa cgtgactgac gcgatcctct
    18661 gcagggcagg agggtcaatt gctgacttta ccgggcatcg gcaaacggtg tttcgcgaac
    18721 tggaacgggt gcttaatttc ccgcaatcaa acttgtgcct taaccgtgag aaacaggacg
    18781 aaagctgttc attaacgcag gcattaccat cggaactcaa ggtgagcgcc gacaatgtct
    18841 cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga gatatttctc ctgcaacagg
    18901 cacaaggaat gccggagccg gggtggggaa ggatcaccga ttcacaccag tggaacacct
    18961 tgctaagttt gcataacgcg caattttatt tactacaacg cacgccggag gttgcccgca
    19021 gtcgcgccac cccgttattg gatttgatca tggcagcgtt gacgccccat ccaccgcaaa
    19081 aacaggcgta tggtgtgaca ttacccactt cagtgctgtt tattgccgga cacgatacta
    19141 atctggcaaa tctcggcggc gcactggagc tcaactggac gcttccaggt cagccggata
    19201 acacgccgcc aggtggtgaa ctggtgtttg aacgctggcg tcggctaagc gataacagcc
    19261 agtggattca ggtttcgctg gtcttccaga ctttacagca gatgcgtgat aaaacgccgc
    19321 tgtcattaaa tacgccgccc ggagaggtga aactgaccct ggcaggatgt gaagagcgaa
    19381 atgcgcaggg catgtgttcg ttggcaggtt ttacgcaaat cgtgaatgaa gcacgcatac
    19441 cggcgtgcag tttgtaatgc ataaaaaaga gcattcagtt acctgaatgc tctgaggctg
    19501 atgacaaacg aagaactgtc taatgcgtag accggaaaag gcgttcacgc cgcatccggt
    19561 cactttcagt tttactcttt ctcggagtaa ctataaccgt aatagttata gccgtaactg
    19621 taagcggtgc tggcgcgttt aatcacacca ttgaggattg cgcctttaat attaacgcca
    19681 gcctgttcca gacgctgcat tgacaagctc acctctttgg cggtgttcaa gccaaaacgc
    19741 gcaaccagca ggctggtgcc gacagagcgt cccacgaccg cagcattgct caccgccagc
    19801 atcggcggcg tatcgacaat caccagatcg taatggtcgt tcgcccattc cagtaattga
    19861 cgcatccgat cgcgcatcag cagttcagac gggttaggtg gcacctgacc gcgagtaatc
    19921 acatcaaagt ctcctttgcc aaaatgctgg atcactttgt tgagctcatc tttacctgcc
    19981 agatattccg acaagccatg ttcattactc acggtaaaca ggttatgcga ataaccacgg
    20041 cgtaagtcgg catcaataaa taacactttt tgatcggact gggcgatcac cgctgccaga
    20101 gttgaactga caaacgtttt accactgtct ggcgtcgcac cggtgatcat cagaatgtta
    20161 ttctcagtct ccatcatagc gaaatgcaga ctggttcgta gcgcacgtac ggcttccaca
    20221 gcagaatccg ccgggttatc caccgccagg aaggggatat ttttagtacg atggcgctgc
    20281 tgattagaaa ataaattttt cttacgcaga cgggtgcgtt tatccagcca ctcggacatt
    20341 gggatagtgg aataaacgct gatgccgtgc tcttccagtt gttccggggc ttctacacca
    20401 cgacgcaaca tcgcacgcgc cagcacggca ctcaccgaaa taaacaggcc aagaataaaa
    20461 ccaagcacca cattcaacgc ttttttcggt ttcactggct gcggctgagt gactgccggg
    20521 tcgataatcc gcacgttacc aatggcactg gatttcgaaa tactcaactc ctgctggcgg
    20581 ttaagtaatt gcagatatac cgcacggccc gcttctacgt cacgacttaa acgcaacact
    20641 tcctgttggg tggaaggcat tgccgatacc cgcttattca ggcgtttgcg ttcttgctcc
    20701 agcgtctggc gtttttccag cagcgcacga taagttgggt gatctttctt atacagctgg
    20761 gagatctctg cctcgcggaa ggtcagctca ttgagttgat tatcaacgtt cacaatctgc
    20821 tcaagaacgg ctttggcttc caggttaagg tcaaccgaat cgcgctgctg gcgataaacg
    20881 ttgagttttt cttccgcctg gtccagctcg ctgcgcactt caggtaactg gcgctgtaag
    20941 aattcaaggc tttgtgaatc ctgcgccgcc tggcgagcga tattctgttg caaatagttg
    21001 ttagcgatgc tgttcagaat acgagtaatc aactggggat catcaccagt catggtaagt
    21061 tccagcatcc cgctttcttt actgcgttcg ctaacggtaa aggtttcctg caatgcgtta
    21121 atcgcttcca gttcggtacg ctggctcagg acaaactgtg ttcctggttt ggccttaatg
    21181 tccgcgatag tcagcgcaac gccatctttt tccagacgct ggccgaccat accattgacg
    21241 gtgaactctt caccttccag tgtatagtgg ccgttttccc caaccgtgag tgtcagttgc
    21301 tgatcctgac cattcagttg tggaatatgc atccagctga tcgccagctc acctagtttt
    21361 tctttggtta atctcgccca gccgcgaccc acaatcggaa aatacttctg ctcaactatg
    21421 tcgcgcagat tcagttcagc aatggtttta ccgagaatca tgcgcgattg cagcagttgg
    21481 atctccggtg cagactcggg cgatgagtta gggatcatat cgctcaggcc gctgagaatg
    21541 gcgttgccct gtttttgctc aacctggacc agagtatctg cctgataaat tggtgtgctt
    21601 aacagcgatc agcgtgaata acgcggtcac gctgataata aacttacggt gatcccataa
    21661 ctcgccgacc agacgaagca gatcgatctc attttcctga gtgctgcctg gtggcgtatt
    21721 catattttta gttgtcatcg tattttataa actcaaatta ccggctgagg cgcttcgccc
    21781 attcctgact ggcgcgctcc aacatactgt agacatgttc aaatgcgtcc tgacttttac
    21841 gatagggatc cgggatctct ttctgttcca gccattgccc aaacagcatt gtcttcccgc
    21901 ggacctcggg agcaattgcg gtaacctgag caatatgttc cgactccatt gccagaatca
    21961 gatcgtaatt tcgtgccatc tcagcagtga gcttacgtcc ggcatgtcct tcaagagata
    22021 cgccatgatt agcggcaaca tctgccgctg tcgcatctgc agggtgtttt accagaccat
    22081 gaacacctgc cgatttgact tttacgcccg gcagacgttt acgcagcaag cgctcgccaa
    22141 tgggcgaacg gcaaatgttg ccggtacaaa ccactaggat tgagttaaat tttagttggg
    22201 ccatgtttga atccaccgtg aagtctctgt caggtcatgc accccggaaa tcgtcggtac
    22261 cagcagagaa atcacacggt tccaacgagc cagcggcgcg gtagtgacat aaacaatgtc
    22321 gtaaggttca agctggaatt ccgttccgag tatcattgcc gatgcgtcct tcgcattcag
    22381 ctgataaata ttggcgattt tgccattctg cttgttttgc gttgcacgaa taacaaaaat
    22441 ccccgtcgca tcagcgacat cctgattcat accctcagcg ttacccagcg cttctgccag
    22501 ggtcataccg ctgcggtcca ttttcagcgt gctctgttta cccacttcac ccatcacgaa
    22561 gactttcaga tcatcgttgc gcggaataaa taaaatatcg cccgggtgca gcagtttgtt
    22621 ctggcgcaag tcgccacgct gcatcagcgc atagaggttc actttggtct ttacgccatt
    22681 ctgggtgagc accacgttgc gccagtctgc atctgcggtc agaccgcccg cggcgttaat
    22741 cgcatccatg atggtcagag gaatattggt aatcggctgc tgacccgatt tgctgacttc
    22801 gccggtgacg tacgcttttt gcgatcggaa cgccgcgacg ctgacatcga cttgcgggct
    22861 ttcaatgact gaatcgagac gcgctgtaat ctcgttgcgt acctgggtaa gtgttttccc
    22921 ggccactttc aggcgaccaa tataggggta gaaaatcgca ccatcggcgt taacccagtt
    22981 gccggtatca ctggcgctac ggtattgccc tgcgggggtg gttagttccg ggtgatccca
    23041 gaccgtaacc atcaacacat caccaatccc gatacgatat tcatagtttg cgatttgctg
    23101 ctccagctcc gggttagcct gcgacataat cggtttggcg cgcagctgat caatcaatcc
    23161 tggcgtgacg ggatagatat tgaccatttt gtccaggtca tactgattgt caggtagttc
    23221 aatgacatcc ttgttactgg tcgataaatt ctgaccagga acaagggtac atgctgtcaa
    23281 agacagaaca gccaatgtca gtaccgaaaa cttaaaaatg ttctttttca ttggtttata
    23341 tttatcagtc aatgtaatgt tatgaattac gtcaggttat tgtccaaccg gtacttctcg
    23401 ctctgccgtc attggatcga gctggtattt gcgtgacagc atttgtccac catcacgcgt
    23461 caatggtgtc cacgaaacca ccgcgcggtt gcggttaggt ccaatggtca tcaagtcgaa
    23521 cggaatcgag atataaaaac ctttactaaa gccgccttcg ccgtagtcat ctttagatac
    23581 gttactgatt gccgcccata cccctaccgc cacgccgctg tcaaagagtt tggcgacgtc
    23641 gatcgttgcg cctttatctt ttgccagata ttgcccaacg ctaagtttca tcagtacgcc
    23701 attgagcgtc ggcgggttcc agtaagccgt cacgaaacct gtgggcgtgg aataatcggt
    23761 gaagcgcatc atgttgtccc agtcacgctg cttcacgtag ttaacgtcca cacccagcgc
    23821 ccagctggca tctagcgggc gataaagaag ctcggaaccg acaccggcat acatcgtttc
    23881 cagataaccg ccatacacct ggccatagaa accattgcct aagtcggcaa agtaattcgc
    23941 ttgcaagttg ttgagataaa cgtcattgcg aacgtaatca cggatatgcg tgcgcacgcg
    24001 gggcagggta gagtccgcgg gtaacagtga agacttaaac ttgtcgtagt tgttgtaaat
    24061 attggtgaaa ataccgtcat caagcagcag gtggtcggta aaccagtagc gggcgctgga
    24121 catcagcccc agctggaaca tatagaaatc ttccgggccg cccagcgact gtgaaagtgt
    24181 tgggttgaaa gagtagctaa agcgatcttc acgaatacgg tagcaccgac caaaggcaga
    24241 aagatcttct gcttcaacac gttgttgttg cagttgctct gatttaccag gcgctgtgcc
    24301 ggccagctgt ttgcgcaggc tggctacgtc ggtttcggta gtcaccatcg ccatatgctc
    24361 gcgcttttgc gtcacgctaa tcttctcaac gccttgcggc aggttattca ccagaatccg
    24421 gttggcacga tcgaccgctt cgcgagagtc acggtatttg tattgctgac cagacatata
    24481 cagcgtctta tcgcgtagct gaatttctgg cgcgtcaaag cccgcgttat acttcagggc
    24541 ggtaagttgg tttgctaccg tggtgtactg caaaccttca gactctggcg caggctgcca
    24601 ggccggtttt ggcgtatcgc gtagggcagg gcgcagatcg ttgaaattgg tccgtaaagt
    24661 gaaaccaaac atcaacgtgt ttccgcgttc ataactgagg ttgaggtctg cccagctggc
    24721 agcgcgataa actgcaccga cgttgaaatg gcttgcctga ggcagtttgc cagcgaaatc
    24781 attctggtaa ttgttgccgt cgtattcgag tttcagacgc aggggattcc acggcgtttg
    24841 atactcaatg ccgccaaaga tggaagccgg gccacgaaag atatcgctaa agctgatatc
    24901 gcccgcatcg tgagactctg cgcgatgaca atatttatcg ctcacccggc aaaacgggtt
    24961 ggtaatattg cccgcattac cggcgtagcc ccatgccatc ccgagggtga aatcaaatgg
    25021 ccccgcttgc ttgctggcca ccagatattc accgtcaaac aaacccgtac cagcaatatc
    25081 acgtttacca aacgccactt gcggtagcca gtaaccttct tcccacaggc gaagtttaaa
    25141 atcgaatgat ttgtctttgt atgactgatc gccgctgaaa tcttcccact ggctatattt
    25201 gcgtgtgcgc acatccgtat aacgaatggt gccttccagc catgggaaca gcgccacgga
    25261 ggtggagtag aaccggtatt gatcgttatc ccggtagttg acgctgaatt caccttccgg
    25321 tgcgatgcgc gcatttggca tctgcaacaa tcctgtgccg ccaaagtctg attgcgacga
    25381 acccagcgga tccgggtagg ttaatacttc agcatgacag gcactggaga cggcaatggc
    25441 caggcagctt aaaagataag aattcttctt catcagtcag gaacacgttg cgttaaaaca
    25501 gacacaatct ggtcatttaa atcagcatat ttttcgggca gtacatgcgc agaaaagccc
    25561 aaccaaagct ggctgccagg cggtggttcc acatgtcttt tattccacag ggcaacaggg
    25621 gcgacgacag tctcaccctc aggcgtaatc accataacgt tattcttgtc tgcgcctgca
    25681 aggcgggggt gatcttgcag ataatcggta acgctacggc ctgcctgcca gggcaattgc
    25741 ccagccccag aaaccgcacc cagcagagta atagtcaccg gacgctgtac ggtatagagc
    25801 gtatagtccc cgaccaacgg gggattgctg ttctcatcca ctcgtacaaa atcagggtcc
    25861 agtttgaccg gtaagcgacc agtgatattg agattgagta actgctggcg cacggattta
    25921 atcgttgccg ccacatcgtc gtcagcttct gcttcccagg aagcaagctg tgccatgaca
    25981 tgttggtagt ctttaagcgc tttagctttc gccgcagagt cggtcagcaa ggcaccaggc
    26041 caccagaggc gatcgcgcag ttgcggttgt gtcaccaact ggacaacatt ttccacgggg
    26101 ccaacggaaa gtgtctgttg ttcgctaggc agataaatag tcaccgttcc ttgcgcaaag
    26161 gcatggggtg tcattacgta aagtacgctg gcaatgaaat acgactgtaa tttattcatg
    26221 gtgcgggttt aagaaacgtc atttctacgg gaataacccc tgcgcctaac atttgccgac
    26281 tttgacgaac ctggccagta gccgaatcga cccagaatgt gttttgccac tgtttttctg
    26341 gcagcgtgga agtcacctct tcctgccaga cattacacag cacattgtga cccgcaatat
    26401 tcagcgtctc taatccagca aaggaaaaag tagaacttag ggtcgcagag cgaaaatggt
    26461 tgtcttcact ccagagaata tcgcgcgtcc agcggctgcc atcttttatt gccagcgcgt
    26521 tacgcagagg ggcccgcccg ggatttgtca cttccagcag attattatta agtttgacgg
    26581 tctttaaaag acgtccattg tgggtaacca gcatggcgtt atcctgggat aaccatttgc
    26641 tttgttcttg ttctatatat cccagtacca caaagattcg ttgcccttca ttaaggcgta
    26701 aatacatcgt ggaataaggc aacgcctgga tctgctgagc cgctacagtg atatcctgat
    26761 tatcgaaaag gctggcgcga aatgtatcga ccatactttg ctggctgtgc gtacatcccg
    26821 caagaaatag tgcgaaaatc gataaaataa gagggcgcac ctttaatcct tgaaaggtta
    26881 attaaaaaaa tgcaaatctc taaagcccgg tacgaatatc gcaaccggat attatttaac
    26941 ttgaatttcg acgcgtttaa gcagaattag cgcgtagtgg acgtggtggt tgaggtgtta
    27001 tgagaaccgc catcgccact gtcatttgct gcaactactg ccaacgtcgc cacaccaaca
    27061 gccgttgcca caccgacaac tgtcgccgtg ccgacagcgc cagcagccac gccactagtg
    27121 gtagccgcag tcgtggttgc acctgtagtg gtcgttgtgg tgccagtagt acccgtcgtt
    27181 gcctcggtcc cgtttgctgc ttcaggggca gcaaacgcag ctggcgtcgt caacatgaag
    27241 gccataagga ttgcagaaag cttgtgtttc ataacttttc ctttattcat cgcatggaca
    27301 atacgtacgc ttgagatcag gattgtctac tttgagtata acatccttgt gaaatctccc
    27361 tttcctcata ccgctagcac tatatactgc gggtgggaaa ggcgcgcaga gggaaatata
    27421 agattgttaa cgatttccct ctggaaatac gacttgtgag cataagataa atcttagaaa
    27481 aatacgaatt aaagataaga aaattgttac gaaagctatt aattattgtt ggtaatatag
    27541 ttttaagtgg tactataact ggctgttaaa tggcatttat aactattagg tgcagagata
    27601 ttcgcttaaa tggattagtt tcttagtgga aaagtaactg atgttataat gtatggtgtt
    27661 gactattgaa ctattccaat aaaaccagtc agctttaaac aagcagcgtc atattaagag
    27721 agataaacat ttgccgctgt tggtcctcgc aggccattta cgcggcaaaa ttccacgcgt
    27781 aatcctggta taagcacttc tgcgtcgcgg ggagtgaatg cggaaatatg gacctggact
    27841 tctttacgac cgtcggaggg gataatgaat cctttgccgc ttttgcgatc aaaggttttg
    27901 acaattcctg tcattttacg ggacaaacaa attccttact gaaaatactg cgctgcacta
    27961 tacggggtta ataaaataaa gccagcgata tttaagaccg ccggacggct aaaataaaat
    28021 ttgcttaatc tcaattatca tgccttaata gctgcgtcgg tttgaaagac agacagcata
    28081 caaagtagtt tactaaagca gttctcatta tcaggcatta tccccttctt ttgagtctct
    28141 ctcctgaaca ctaagtggtt tctgtattaa agccctgttt gccgaaaggc ccaaaatgaa
    28201 ggaagtaaaa tatgtctaat aa
//
LOCUS       DAJTPG010000049        28219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTPG010000049 DAJTPG010000000
VERSION     DAJTPG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            563..1333
                     /locus_tag="OO230_002172"
     CDS             563..1333
                     /locus_tag="OO230_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5691924.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="OO230_002173"
     CDS             1324..1797
                     /locus_tag="OO230_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5691925.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="OO230_002174"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="OO230_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5691926.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="OO230_002175"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="OO230_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5691927.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="OO230_002176"
     CDS             3232..3726
                     /locus_tag="OO230_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5691928.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="OO230_002177"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="OO230_002177"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5691929.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="OO230_002178"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="OO230_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="OO230_002179"
     CDS             7556..8470
                     /locus_tag="OO230_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5691930.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="OO230_002180"
     CDS             8685..10046
                     /locus_tag="OO230_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5691931.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="OO230_002181"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="OO230_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5691932.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="OO230_002182"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="OO230_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5691933.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /locus_tag="OO230_002183"
     CDS             12596..13885
                     /locus_tag="OO230_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5691934.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="OO230_002184"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="OO230_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5691935.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="OO230_002185"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="OO230_002185"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5691936.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="OO230_002186"
     CDS             16211..16591
                     /locus_tag="OO230_002186"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5691937.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="OO230_002187"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="OO230_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5691938.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="OO230_002188"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="OO230_002188"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5691939.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="OO230_002189"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="OO230_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5691940.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="OO230_002190"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="OO230_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5691941.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20580..21517
                     /locus_tag="OO230_002191"
                     /pseudo
     CDS             20580..21517
                     /locus_tag="OO230_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="OO230_002192"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="OO230_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="OO230_002193"
     CDS             23320..23832
                     /locus_tag="OO230_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5691942.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="OO230_002194"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="OO230_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5691943.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="OO230_002195"
     CDS             26425..26628
                     /locus_tag="OO230_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5691944.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="OO230_002196"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="OO230_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5691945.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28219)
                     /locus_tag="OO230_002197"
     CDS             complement(27664..>28219)
                     /locus_tag="OO230_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5691946.1"
                     /translation="KKQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAA
                     LDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINR
                     FQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSS
                     EWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6886 a         7382 c         7099 g         6852 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attggatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccaa gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcctgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttt
//
LOCUS       DAJTPG010000050        27364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTPG010000050 DAJTPG010000000
VERSION     DAJTPG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO230_002198"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO230_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5691947.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO230_002199"
     CDS             complement(1614..1937)
                     /locus_tag="OO230_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5691948.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO230_002200"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO230_002200"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5691949.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO230_002201"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO230_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5691950.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO230_002202"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO230_002202"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5691951.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO230_002203"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO230_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5691952.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO230_002204"
     CDS             7537..8574
                     /locus_tag="OO230_002204"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5691953.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO230_002205"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO230_002205"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5691954.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO230_002206"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO230_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5691955.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO230_002207"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO230_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5691956.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO230_002208"
     CDS             10745..11626
                     /locus_tag="OO230_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5691957.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO230_002209"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO230_002209"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5691958.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO230_002210"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO230_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5691959.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO230_002211"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO230_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO230_002212"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO230_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5691960.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO230_002213"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO230_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5691961.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO230_002214"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO230_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5691962.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO230_002215"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO230_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5691963.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO230_002216"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO230_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5691964.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO230_002217"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO230_002217"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5691965.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO230_002218"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO230_002218"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5691966.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO230_002219"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO230_002219"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5691967.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO230_002220"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO230_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5691968.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO230_002221"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO230_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5691969.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO230_002222"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO230_002222"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5691970.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO230_002223"
     CDS             24893..25273
                     /locus_tag="OO230_002223"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5691971.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO230_002224"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO230_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5691972.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO230_002225"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO230_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5691973.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27364
                     /locus_tag="OO230_002226"
                     /pseudo
     CDS             26442..27364
                     /locus_tag="OO230_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6743 a         6812 c         7176 g         6633 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctg
//
LOCUS       DAJTPG010000051        26648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTPG010000051 DAJTPG010000000
VERSION     DAJTPG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(48..188)
                     /locus_tag="OO230_002227"
     CDS             complement(48..188)
                     /locus_tag="OO230_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5691974.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            430..723
                     /locus_tag="OO230_002228"
     CDS             430..723
                     /locus_tag="OO230_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5691975.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            795..1454
                     /locus_tag="OO230_002229"
     CDS             795..1454
                     /locus_tag="OO230_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5691976.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1546..1992
                     /locus_tag="OO230_002230"
     CDS             1546..1992
                     /locus_tag="OO230_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5691977.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2199..3099)
                     /gene="hlyE"
                     /locus_tag="OO230_002231"
                     /pseudo
     CDS             complement(2199..3099)
                     /gene="hlyE"
                     /locus_tag="OO230_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3472..3891
                     /locus_tag="OO230_002232"
     CDS             3472..3891
                     /locus_tag="OO230_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5691978.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3891..5159
                     /gene="umuC"
                     /locus_tag="OO230_002233"
     CDS             3891..5159
                     /gene="umuC"
                     /locus_tag="OO230_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5691979.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5205..5735)
                     /gene="dsbB"
                     /locus_tag="OO230_002234"
     CDS             complement(5205..5735)
                     /gene="dsbB"
                     /locus_tag="OO230_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5691980.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5881..7422)
                     /gene="nhaB"
                     /locus_tag="OO230_002235"
     CDS             complement(5881..7422)
                     /gene="nhaB"
                     /locus_tag="OO230_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5691981.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7643..8362
                     /gene="fadR"
                     /locus_tag="OO230_002236"
     CDS             7643..8362
                     /gene="fadR"
                     /locus_tag="OO230_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5691982.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8414..9946)
                     /locus_tag="OO230_002237"
     CDS             complement(8414..9946)
                     /locus_tag="OO230_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5691983.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10276..11574
                     /gene="dadA"
                     /locus_tag="OO230_002238"
     CDS             10276..11574
                     /gene="dadA"
                     /locus_tag="OO230_002238"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5691984.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11584..12654
                     /gene="dadX"
                     /locus_tag="OO230_002239"
     CDS             11584..12654
                     /gene="dadX"
                     /locus_tag="OO230_002239"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5691985.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12822..14236)
                     /locus_tag="OO230_002240"
                     /pseudo
     CDS             complement(12822..14236)
                     /locus_tag="OO230_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14847..16583)
                     /locus_tag="OO230_002241"
     CDS             complement(14847..16583)
                     /locus_tag="OO230_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5691986.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16678..17592)
                     /gene="ldcA"
                     /locus_tag="OO230_002242"
     CDS             complement(16678..17592)
                     /gene="ldcA"
                     /locus_tag="OO230_002242"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5691987.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17692..18303
                     /gene="emtA"
                     /locus_tag="OO230_002243"
     CDS             17692..18303
                     /gene="emtA"
                     /locus_tag="OO230_002243"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5691988.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18305..19039)
                     /locus_tag="OO230_002244"
     CDS             complement(18305..19039)
                     /locus_tag="OO230_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5691989.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19240..19494
                     /locus_tag="OO230_002245"
     CDS             19240..19494
                     /locus_tag="OO230_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5691990.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19544..21004)
                     /locus_tag="OO230_002246"
     CDS             complement(19544..21004)
                     /locus_tag="OO230_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5691991.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20941..21498)
                     /locus_tag="OO230_002247"
     CDS             complement(20941..21498)
                     /locus_tag="OO230_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5691992.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21524..22378)
                     /locus_tag="OO230_002248"
     CDS             complement(21524..22378)
                     /locus_tag="OO230_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5691993.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22378..23187)
                     /locus_tag="OO230_002249"
     CDS             complement(22378..23187)
                     /locus_tag="OO230_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5691994.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23197..23955)
                     /locus_tag="OO230_002250"
     CDS             complement(23197..23955)
                     /locus_tag="OO230_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5691995.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23952..24932)
                     /locus_tag="OO230_002251"
                     /pseudo
     CDS             complement(23952..24932)
                     /locus_tag="OO230_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(24932..25954)
                     /locus_tag="OO230_002252"
     CDS             complement(24932..25954)
                     /locus_tag="OO230_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5691996.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26304..>26648
                     /locus_tag="OO230_002253"
     CDS             26304..>26648
                     /locus_tag="OO230_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5691997.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNILH"
BASE COUNT         6694 a         6491 c         6878 g         6585 t
ORIGIN      
        1 tggtgtggcg gcctcagtcc ggaacaattt gaaaaccaga acctcgctta ggcctgtgtc
       61 catattacgt gggtaggatc actatggcct ttgctgaact caacattgac attcttaccc
      121 gcagcaaagc tcgtggaggt tagcagtgca agcagcaata cagccttact gatgctcttg
      181 attttcattg gtagtctcct gttcagaatg agtacgatga actgttataa tataacaatc
      241 ccttacggga gaattccagt ggcttttctg cgttcggtaa aatcccggcg cggcattttt
      301 tgccgggggg attacttata aaggggcatg gactcaacaa accgcagaaa tctacagctg
      361 gctggcttgt ggcgcgggtt tcattgatgt tagcttatgc caaaacctgg catcttaaag
      421 agtaaaagta tgttttgtgc gatttatcga agcagcaagc gtgaccagac ctatttatat
      481 gtcgaaaaaa aagacgattt ttcgcgtgtt cctgaggaac tgatgaaagg ttttggtcag
      541 cctcagttag cgatgattct gccgttggat gggcggaaga aactggtcaa tgctgatatt
      601 gaaaaggtta aactggcatt aaccgagcaa ggttactatt tgcagttacc gccaccaccc
      661 gaagatttgc tgaagcaaca tctttccgtc atggggcaga aaacagacga cactaacaaa
      721 taaccgatat ccggcggtgg cattatcttt gtcggcgcgg gttttcatat ccacgataag
      781 gtgaggggaa cgttatgtat caacatcaca actggcaagg tgcgctgctg gattatccgg
      841 tgagtaaagt agtctgtgtt ggcagtaact atgccaaaca tattaaagag atgggcagcg
      901 cagtgcccga agagccagtg ctgtttatta aaccagaaac ggcactgtgc gatctgcggc
      961 agccattggc gatcccatcc gatttcggtt cagttcatca tgaagtcgaa ctggcggtgt
     1021 tgattggcgc gacgctgcgt caggcaacgg aagagcatgt ccgcaaagcc attgccggtt
     1081 acggcgtggc gcttgatctg acattgcgtg atgttcaggg aaaaatgaag aaagccgggc
     1141 agccgtggga aaaggctaaa gcgtttgata actcttgtcc gctttccggg tttatccccg
     1201 cggcggaatt taccggcgat ccacaaaata caacgctggg cctgagcgta aacggcgaaa
     1261 aacgccagca aggtacgact gcggacatga tccataaaat cgttccgttg atcgcttata
     1321 tgagcaagtt ttttaccctc aaggccggtg acgttgtgtt gacaggcacg cctgatggcg
     1381 tcggcccgtt gcaaagcggt gatgagctga cagtcacttt cgatgggcat tctttgacaa
     1441 ctcgcgtttt gtaatacttt ttgccgccag aaagcggcgg caaaacttgc atcgctgtgc
     1501 cagactggtt ataaggtgcg ttttaacgta atggcggaac acctgatgag cgatgtacct
     1561 ttctggcaaa gtaaaaccct ggacgaaatg agcgatgcgg aatgggagtc gttgtgtgat
     1621 ggttgcggtc agtgttgctt gcataaactg atggatgaag acaccgacga aatctacttc
     1681 actaacgtcg cctgtcgcca gctcaatatt aaaacctgtc aatgtcggaa ctacgaacgt
     1741 cgtttcgagt ttgaacccga ctgcattaaa ttaacccgtg aaaatctgcc aacattcgaa
     1801 tggctgccaa tgacctgcgc ttatcggttg ctggcggaag gtaaagattt acctgcgtgg
     1861 catccgctac ttactggttc gaaagcggca atgcatggtg aacgtatctc tgtgcgtcat
     1921 atcgcagtga aagaatcaga agtgattgac tggcaggatc atatcttaaa taaacctgac
     1981 tgggcgcagt gataaattta atagatgctg atgggggctt tcttaatata cgtctggatg
     2041 ctcgccattt cgtagccctg tttttgaaag cgctctgtca taatgagagt tacccgatac
     2101 cttattatca acaggtaact ctcaatcttt aaaaaacgtc tgtgttttta aatgtgataa
     2161 accttatacc ttgagtacat ggagagaata atcgcttatc agacttcagg tacttcaaag
     2221 agtgtctttt taccgtgtct tttctgatac tcattacagg tgttaatcat ttttttggcc
     2281 gctgctttta gcaaagaaag cattaaatca tcataatcaa catagaatct ggttgtttca
     2341 gtttccgttt ttatctcccc gatggcggct atttcggtgg ttaatttcaa tttggcggca
     2401 tcgatatctt tattcgcttg tttaaccgtg ttagacaggg tggtaaagaa actctgcaca
     2461 gattttaact tgttcttcaa ttctggaatc agtttccctt caactacgcc cgcagcaata
     2521 gaataggaaa tgattaaacc aaatggaccg gcgacgacat cggctgcggc accggcatac
     2581 gcttccttcc tgattttatc tacctgtgac tggaaatagc tgcttttttc tgaaaaatca
     2641 ttggttaact ggctatctaa cgccagcagt ttcccggaag cgttgttgaa actttgtgag
     2701 cttaccagca gggatttttg cgcttcattc agcttcgtga tgccgtcatc cagtacctta
     2761 atgaggggcg gatgctttct tctcattgta ctcatcaaat agcaaaatat acgctgcgag
     2821 caattgcgtc gcaacaccac accattcata cactgtttgg gttgcttcaa aatacttatc
     2881 ctggctatcc ataagtaagg ttttaatatc gccgactaaa acggaggctg cctgtgaata
     2941 ctcctgttta aagcgactta actcttttat ggtttcatca aaggtctgcc aggggatgac
     3001 ctgatcgaga tatttattat aaagatctaa tgctccatct gcggtttcga ttgcgttttt
     3061 aactacttct accgttttat ctgcaacgat ttcagtcata atcattcgcc tctttaaata
     3121 tataaattgt aatgaaactc ctgttttaca actattaata aattttactt catctaattc
     3181 atagttagcc gggcgggatg cgtcaatgtc tttatttcta ttaatatgat aaatatcaaa
     3241 caatgtttaa tgtcattatg gcgaatgctt ctattctatt tttttgccgg gtgatatttt
     3301 tcatttctgc tggatgagcg tcgtcgccag aaggccacgt gagcacagga taagagaacg
     3361 aaaaatcagc agtctatgca gcgacaaata ttgatagcct gaatcagtat tgatctgctg
     3421 gcaagaacag accactgtat ataaaaacag tataacttca ggcagattat tatgttgttt
     3481 atcaagcctg cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag
     3541 tgtggctttc cttcaccggc agcagattac gttgaacagc gcatcgatct gaatcaactg
     3601 ttgatccagc atcccagcgc gacttacttc gtcaaagcaa gtggtgattc tatgattgat
     3661 ggtggaatta gtgacggtga tttactgatt gtcgatagtg ctattaccgc cagccatggt
     3721 gatattgtca tcgctgctgt tgacggcgag tttacggtga aaaaattgca actacgcccg
     3781 acggtacagc ttattcccat gaacagtgcg tactcgccca ttaccatcag tagcgaagat
     3841 acgctggatg tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg atgtttgccc
     3901 tctgtgatgt aaacgcgttt tatgccagct gtgagacggt gtttcgccct gatttatggg
     3961 gtaaaccggt ggttgtgcta tcgaataatg acggttgcgt tatcgcccga aacgctgagg
     4021 caaaggcgct gggcgttaaa atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc
     4081 gctgtggtgt ggtttgcttt agcagcaatt atgagcttta cgcagacatg agcaatcggg
     4141 tgatgtcgac gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt gatgaagcat
     4201 tctgcgatct gacaggtgtg cgtaattgtc gcgatctgac tgattttggc agagaaattc
     4261 gcgcaacggt gctacaacgt acccatctta ctgttggtgt ggggatcgcc cagaccaaaa
     4321 cgttggctaa gcttgccaat catgcggcaa aaaaatggca gcggcagacg ggtggggtgg
     4381 tggatttatc aaatctggaa cgccagcgta aattaatgtc tgctctcccc gtggatgacg
     4441 tctgggggat tggacggcgg atcagcaaaa aactggacgc gatggggatc aaaaccgttc
     4501 tcgatttggc ggatacagat atccggttta tccgtaaaca ttttaatgtc atgctcgaaa
     4561 gaacggtgcg tgaactgcgc ggcgaaccct gtttgcaact ggaagagttt gcaccgacga
     4621 agcaggaaat tatctgttcc cgctcgtttg gtgaacgcat cacggattat acgtcgatgc
     4681 ggcaggccat ttgtagttac gctgcccggg cggcggaaaa acttcgcagt gagcatcaat
     4741 attgtcggtt tatctccaca tttattaaga cgtcaccatt tgcgctcaat gaaccttatt
     4801 acggcaatag cgcgtcggta aaactgctga cgcccactca ggacagcagg gatatcatta
     4861 acgctgctac gcgatctctg gatgccatct ggcaagcggg ccatcgctac caaaaagcgg
     4921 gcgtgatgct gggggatttc ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca
     4981 acgcaccgcg ccccgggagt gagcaattga tggcggtaat ggatactctg aatgctaaag
     5041 agggcagagg aacactctat tttgccgggc aggggatcca acaacaatgg cagatgaagc
     5101 gagctatgct ttcaccacgt tatacaacgc gaagttctga tttactgcgg gtcaaataaa
     5161 tatagcggca ggaaaaaagc gctcccgcag gagcgccgaa tggattagcg accgaacaga
     5221 tcacgttttt tcgctttaaa cggctgggaa atcaccacca gcaccgcgac aatcaggtaa
     5281 gcgataaaaa taccgagcag ccactgcggc atttccatac ctaaaaaatc ccactgacgc
     5341 tcggcgcaat cgccagaggc gacaaacact tgcggcaccc atttatccag cggcagccat
     5401 tccgggaaac gaaccataaa atcacaggtg gcaaatggcg aaggatagag ctgaagcatg
     5461 gtgtgctcgt aagttaactg cacaccgcgg aacgcactat acaaccagat aaccatcgct
     5521 acataacgca gcggagtttt cggggcgatc gcgccaatca gcgcagcacc cagaacgccg
     5581 aataacgcgc agcgttcata aatacagagc acgcaaggtt tcagtaacat cacatgctgg
     5641 aaccacagcg ccgtcagttc cagtgccaga gcagtaaacg ccatcaacag ccacgcgccc
     5701 cggccttgtg aacattggtt caaaaatcgc aacataatca tttccctgca atatgcatag
     5761 agtgcgcagt ttaaaccaat tcattcgctg cgccaccagg aggcagcaaa aataggacgc
     5821 aattggacaa ttatcgggcg aaaaggcaaa ccgggcattt atatgcccgg taagttgtta
     5881 tcaaagcgtt gctatccagc ccatttgcat aaaccattcg gttacagggg caagcgtaaa
     5941 ctcgacgcag agcaagccga cgagtgtcag tacgagggtg taaggcaggg ccatccacac
     6001 catgcggcca taagagaggc gaatcaatgg cgcgagtgca gaggtcagca ggaacaggaa
     6061 cgcagcctga ccgttcggcg tagcgacaga gggcagattg gtaccggtat taatggcgac
     6121 cgccagcagc tcgtattgct tcaacgtgat agcgccactt tccattgccg ctttcgcttc
     6181 gttgatataa atcgtcccca cgaagacgtt atccgaaatg gatgacagca gaccgttgaa
     6241 aatatagaac agcgacagct gagcatgttc agatgcctgc aacacaaact gaataatcgg
     6301 cgaaaacagt tgttggtcga taatcaccgc gactaccgag aaaaacaccg tcaacagtgc
     6361 ggtgaatggc agagattcgg tgaaggcttt accgatagca tgttcatcgg tgacaccggt
     6421 caatgatgtt gccagaataa tgactgacaa accaatcaag ccaacttccg ccagatgcaa
     6481 cgccagcgca gtcaccagcc agacgccaat aatcgcctgg acaatcagac ggattttatc
     6541 ctgacgggta cgctggtggc ggctttgatc gtcaaactgt tgcaacactt cgcggacttt
     6601 ctccggcagc gtttcaccgt agccaaacca acgcagcttc tctaccagca ggcaggttaa
     6661 caggccacaa atcagaaccg gaacggtcac cggcgacatg cgcaggaaga aatcgccaaa
     6721 atgccagcca gccgctttag cgatgatcag gttctgtggt tcgcccacca tggtcattac
     6781 gccgcctaat gcggtgccga caccggcatg catcatcagg ctacgcagaa agccacggaa
     6841 ctgttccaga accacgttgt aatgcttgtc gatatgacta tcgtcttgca ggtcggtgtc
     6901 ttcggtacgg gaagaggcta cgcgatgata aataccataa aaaccgactg caacgctgat
     6961 caccaccgcc acgacggtta aggcatcgag gaacgcggag aggaacgctg ccgccacgca
     7021 aaaagagagc gacagcagca ttttggagcg aatgcttaac agcaaacggg taaatatgaa
     7081 cagcaacagc tgtttcataa aatagatacc cgccaccata aacatcaata acagcaagac
     7141 ttcaagattt gccgccacct cttcacggac gtgttccgcg ctggtcatgc cgatgaatac
     7201 cgcttcgata gccaacagac caccggggag tagcgggtag catttcaggg ccatcgccag
     7261 agtgaaaata aattccgcta ccagcaacca gcccgcgacg aaagggctga tgaggaaaat
     7321 taacgggttt acgattaaga aaatgatgag ggcgagtttg taccagtcgg gggactggcc
     7381 caaaaaattg cgccatagcg cgcggcccca ggagatctcc atgatggttt cccttacctt
     7441 acaaataatc aatgatgttt ttatgtttaa acgcaaagct taacggtcag gcaggagtga
     7501 ggcaagtctt gatagtcaag gggaaagaga tgcggaaaat gaagccttga tccctttttc
     7561 ttctttttgt ctgctatcag cgtagttagc cctctggtat gatgagtcca actttgtttt
     7621 gctgtgttat ggaaatctca ctatggtcat taaggcgcaa agcccggcgg gtttcgcgga
     7681 agagtacatt attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc
     7741 agaacgtgaa ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca
     7801 gcgtctggca cgagatggct ggttgaccat tcaacatggc aagccgacga aggtgaataa
     7861 tttctgggaa acttccggtt taaatatcct tgaaacactg gcgcgactgg atcacgaaag
     7921 tgtgccgcag cttattgata atttgctgtc ggtgcgtacc aatatttcca ctatttttat
     7981 tcgcaccgcg tttcgtcagc atcccgataa agcgcaggaa gtgctggcta ccgctaatga
     8041 agtggccgat cacgccgatg cctttgccga gctggattac aacatattcc gcggcctggc
     8101 gtttgcttcc ggcaacccga tttacggtct gatccttaac gggatgaaag ggctgtatac
     8161 gcgcattggt cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta
     8221 ccacaaactg tcggcgttgt gcagtgaagg cgcgcacgat caggtgtacg aaacagtgcg
     8281 tcgctatggg catgagagtg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga
     8341 tttagccatt caggggcgat aatcccttcc gtttaaagag caaccccctc aaacgagggg
     8401 ttttttgttg tttttacaga tttcccattc ttggcgggca acgttccagc aactcgatgc
     8461 tgccgtcttc gttttgctgt tcgagcatca catcaaatcc ccacaggcga tgcacatgct
     8521 tcaggacttc tttgcgcccc cgatccagcg gtgcgcgatt atgtggaata taacgcagcg
     8581 tcagcgaacg gtcgccgcgc aaatccacgt tccagatctg aatattcggc tccagattac
     8641 ttaagttata ttgcgacgat aaccggttac ggatctcccg ataaccttct tcattatgaa
     8701 tagcggaaat ctccagataa ttatgccgat catcatccag cacggtgaag aagcggaaat
     8761 cacgcatcac tttcggtgac agaaactggc tgataaaact ctcatctttg aaatcacgca
     8821 tcgcgaaatg cagcgtttcc agccagtcgg aaccggcgat atccgggaac cagtatttgt
     8881 cttcttccgt tggcgactga caaatccgtt taatatcctg gaacatggcg aacccgaggg
     8941 catacgggtt gatgccgctg taccacgggc tgttataggg gggctggaag accacattgg
     9001 tgtggctgtg caaaaactcc agcataaaac gttccgttac tttcccttca tcatacagat
     9061 ggttaaggat ggtatagtgc cagaaggtcg cacagccttc gttcatcacc tgagtctgtt
     9121 tttgcggata aaaatactgg ctcaccttac gcacaatacg caggatttca cgctgccatg
     9181 attccagcag tggcgcattt ttctccataa aatagagcag gttttcttgt ggttcggacg
     9241 gatagcggcg cgcttcagca accgttttct cttcctcgcg cttcggcagg gtacgccaga
     9301 gcatattgac ctgactttgt agatactctt cgcgactttt ctgccgggct ttctcttctt
     9361 gcagcgagat tttttgcggg cgtttgtacc ggtccacgcc gtagttcatc agcgcgtggc
     9421 acgagtccag aagccgttct acttcatcaa cgccataacg ttcttcgcac tcggtaatat
     9481 atttacgggc aaaaatcaga taatcgacaa tcgaactggc gtcggtccaa ctgcggaata
     9541 agcaattgtt tttaaagaaa gagttatgtc catagcaggc gtgtgccatc accagcgcct
     9601 gcatagtaat ggtgttctct tccatcaagt aagcgataca cgggttagag ttaatgacga
     9661 tttcataggc cagcccttgc tgaccgtgct tatacagccg ttcagtctcg ataaactttt
     9721 taccgaatga ccagtgcgga tagttaattg gcatgccgac gctggaatag gcatccatca
     9781 tctgttctga ggttatcact tcaatctggt gcgggtaggt atccagccgg tagagtttcg
     9841 ccacccggtc tatctctgcc agataaacat ccagcaggtc gaacgtccag tcgggtccat
     9901 cgctcaaacg tgtggtgtcc ttattcatag aatcgatcgt cgccatacgc gcacctcatt
     9961 gttgtcggcg ctctctgtgt ggagcacctc atttcaagca tagaacacct gttaaaaacc
    10021 gcgtcgccgg agaatttttt tctttgcgat ttcttattat cagagtgcca ctaatccgct
    10081 tctgaacgga attttatgct ggataaaaag ggcgttcagc aggagatact aaagacgcca
    10141 tattgccgca gagtcaggga gatgtgagtc agctcaccat aaaaaagccg tatgttgaat
    10201 aatattttca actgagttat caagatgtaa ttagattatt attcttttac tgtatctacc
    10261 gttatcggag tggctatgcg agttgtcata ctgggaagtg gtgtggtagg cgttgccagc
    10321 gcctggtact taaatcaggc aggacatgag gtcaccgtca ttgatcggga gccgggggcc
    10381 gccctggaaa ccagtgccgc gaatgccggg caaatctccc ccggatatgc tgcaccgtgg
    10441 gcagcaccag gtgtgccttt aaaagcgatt aaatggatgt tccagcgcca tgcgccgctg
    10501 gcggttcgtc tcgacggtac gcagtttcag ttgaaatgga tgtggcaaat gttacgtaac
    10561 tgcgacacca gccactacat ggaaaacaaa gggcggatgg tgcgtctggc ggaatacagc
    10621 cgtgattgcc tgaaagcatt gcgcgccgaa accaatattc agtatgaagg gcgtcagggt
    10681 gggacgctgc aactgttccg taccgaacaa cagtatgaaa atgcgacccg cgatatcgcc
    10741 gtgctggaag atgccggcgt accgtatcag ctgctggaat ccagccgcct ggcggaagtg
    10801 gatcccgcgc tggcagaagt agcgcacaaa ctgacgggcg gcctgcagtt acccaatgat
    10861 gaaaccggag actgccagct gtttacccag aatctggcgc ggatggcaga gcaggcgggg
    10921 gttaaattcc gctttaatac gcccgttgac caactgcttt gcgacggcga gcaaatctac
    10981 ggcgtgaagt ttggcgatga agtgattaag gccgatgcgt atgtgatggc gtttggttct
    11041 tactcgacgg cgatgctcaa aggcattgtt gatattccgg tgtatccgct gaaaggctac
    11101 tcgctgacta ttccaattgc gcaggaagat ggtgcgccag tatccaccat tcttgatgaa
    11161 acctacaaaa tcgccattac ccgtttcgat aaccgcattc gtgttggcgg aatggcggaa
    11221 attgttggtt ttaacaccga gctgttgcaa ccgcgtcgtg aaacgctgga aatggtggtt
    11281 cgcgatctct atccacgcgg cggtcatgtc gagcaggcga ctttctggac gggtttgcgc
    11341 ccgatgacgc cagacggtac gccggttgtc gggagtacac gctttaaaaa tctgtggctg
    11401 aataccggtc acggcacgct cggctggacg atggcttgcg gttccggtca gttgttaagc
    11461 gatctgctct ctggtcgcac gccagcgatc ccatatgagg atctaagcgt agcgcgctac
    11521 agccgtggat ttacgccatc acgtccgggc catttacatg gcgcacacag ctaaggaaac
    11581 gagatgaccc gtccgataca ggccagcctc gatctgcagg cattaaaaca gaatctgtcc
    11641 attgtccgcc aggccgcgcc gcacgcgcgc gtctggtcgg tggtaaaagc gaacgcttac
    11701 gggcatggta ttgagcgtat ctggagcgcg ctcggggcca ccgatggctt tgcattactt
    11761 aacctggaag aggcaataac gttacgtgag cgcggctgga aggggccgat cctgatgctg
    11821 gaaggatttt tccatgctca ggatctggag atttatgacc agcaccgcct gaccacctgc
    11881 gtacacagca actggcagct caaagcactg caaaatgcgc ggctaaaagc accgttggat
    11941 atttatctta aagtgaacag tgggatgaat cggttgggct tccagtccga tcgcgtgctt
    12001 accgtctggc agcagttgcg ggcaatggcg aatgttggcg aaatgaccct gatgtcgcat
    12061 tttgctgaag cggaacatcc tgatggaatt tccggcgcga tggcgcgtat tgagcaggcg
    12121 gcggaggggc tggagtgtcg gcgttcgttg tccaattcgg cggcgactct gtggcacccg
    12181 gaagcgcatt ttgactgggt tcggcctggc attattttgt atggcgcttc gccgtccggt
    12241 cagtggcgtg atatcgccaa taccggatta cgtccggtga tgacgctaag cagtgagatt
    12301 attggtgtcc agacgctaaa agcgggcgag cgtgtgggct acggcggtcg ctatactgcg
    12361 cgcgatgaac agcgaatcgg cattgtcgcc gcaggatacg ccgacggtta tccgcgccat
    12421 gcgcctaccg gtgctcctgt tttagtggac ggcgtgcgca ccatgacggt ggggaccgtc
    12481 tcgatggata tgctggcggt tgatttaacg ccttgcccgc aggcggggat tggtacgccg
    12541 gttgagctgt ggggcaagga gatcaaaatt gatgatgtcg ccgccgctgc cggaacggtg
    12601 ggctatgagt tgatgtgcgc actggcgtta cgcgtcccgg ttgtgacagt gtaacttgtt
    12661 gtaagccgga tcggaggcag cgtcttctgg ttgcgaaaaa atcatccatc cggctggtca
    12721 gcaactgtag ttgttaatgt gacagagcca ttgcccatga tagtgtccat taaaagaatg
    12781 gacactattt ccccggaacc tgaactcgcc gcacaggcgt tctacataaa acgcttacgc
    12841 ttcattgttg actcggagtc gacttcagat aaatcacgct tcacccttga tgcagattcg
    12901 ggttcatgtt cagattgttg attaacaggc tgcgagtcct gttgtctttc cagactcaac
    12961 aggcccaact gttgatccag gagctccagg tcctcctgta ccttcacgcg ctccaggagt
    13021 tccgggtcat ccaggaactc cagatccata aggtcctcca gctcctcgag tccttccagg
    13081 tcaacctagc cttccttgtg caaaaagtcc aaaaggtcct tcaggccctg ctgttttttc
    13141 aggccctcga cggctttgag cagcgtcggt aaattgtctt tgtcccacgg gttaccatta
    13201 cagtcaaaca ccttcaggct ctggggctgc ggcacctgta ccgttttaaa cttgttttct
    13261 ccacagtagt aaattattag tttgggcggc agccgtggtg aagagatcaa atcgttacta
    13321 gaacagtcaa atactttcag gcgagtcaag ttctccagca tctcatcagg tattgagtta
    13381 atattgttgc cggcacagga tagtttatac aggtactggg gcatcgacgg cggtaaaaat
    13441 tgcaagaagt tttcagaaca gttaatcact ctcaggttag tcgacagctc aggcagccag
    13501 ataatactgt tttcatagca cttcagtacc atcagttttg agggctgcac cttcggtaac
    13561 aagatcaaga cgttatctgt acagtcaagc accagcagcc actttggcat caccttcggt
    13621 aacgcggtca acacgttttt actgcagtaa aattcaatca gatgtggagg caaattctca
    13681 gggagtgaag tcaatcccag gttgctaaga ttgagcatac tccgtcttgt aatgagacat
    13741 tccttcattc tgcgcaatgc ttctgcccgg ttttcaatcc ggcttgctgg caggttttct
    13801 tccaggatat tttccttgat ttcttccaga ctttctttca ggtcttcttg gggaatttct
    13861 gactgtttgt tttgctggtt ttcttccagg atttcgtaca ggtttttttg caggttttct
    13921 tgcaaaccat cctgctgtat agcccattcg caccatttgg cgaccatcat gaactcgtta
    13981 tgttgctgat tcagacgagt ccccaacgtg atgatggtat tgttgtccac ctgtatgttc
    14041 actttgcccg cgatactgag gtcaatgatc tgccaggggg catcacccca gctgaatttt
    14101 tgcgggctga gatacttcat gttctgcaaa atttgattct tgagaagagc cgccctggcg
    14161 ggcgtcgtac gcctgggtta acggaagatg ggggaatggc gggggcgaat gggcagatgt
    14221 ctttaaagct agccataatg ctacgtttcc ttgaaataaa tatcctctgg cagcgccgga
    14281 gacgtgaatg tggtcaccgc aaaggagcca caggacgtca tttgcgctga tatcactact
    14341 catcgttgac ttttttcctg gtcagaaatg ccgtgtcggg tctggtacat gctgtattac
    14401 ggcctaggta gctctgacgt attaaagaaa atacgagagt gaacgacccg gaaataatcg
    14461 ttattaagca aaggaataac tataagaaat cgctcatgca tccggtgcta caggcggatg
    14521 aaacgcctat tgtgtgggct atgtcagtgc ggcaggttgc gtgggggaat atgttcaggc
    14581 gatgtcaggc aggggcccct gatggttgac cggtaatgcg gggctggttt ttattacctg
    14641 ttacagtcgg aaaaagccct tacgctgact ttcttgagcc ggtgttttga gttcattagc
    14701 cagagcctgc aatggtttgt catccataat ttaaccttct tctgatgttg ggatgagcat
    14761 atcaaaatca ggaaatgaca agtttattac aggctcaggc tgcgttcagt aaagcatcgc
    14821 atccggcgat agatgctgca ttaaatttaa gattcagctt cctcttcagc cacccgaacg
    14881 ccaatcttca acacttcatt atcttctttc tcggccaccg tccagatcat cccggcaaac
    14941 tctacctggt caccgacaac cggtgccgcg cctagcaact gctggacaat ttcacccagc
    15001 gtttgctgtt tatcacgata ttctcgcccg tcttccaggc catatatcag cgcaacatca
    15061 gcatatttgg cgctggcttc gagaatgaag tcaccaaaga agcgttgatc cagcgcgacc
    15121 ggtggcgact ggctgaacag tttaccgagc gccgggagat cgcgttcccg accaattaca
    15181 cacaatacat cgccttcgcg cagtcgggtg ctgccggtag gatgaagcaa ctggttatca
    15241 cgaaacaggg ctgcaatacg cgtctctttt ggcatatgca aatcgcgcag tgccgcacct
    15301 acacaccatt tatcagcact caattgataa acaaactgct cccacggatt ttccggatga
    15361 atatccaggc caacgcgtga caccggacgt ccgacgggcg gaaccaccac tttggctttt
    15421 ttcgccgccc acgagagtga tgttccctgc aagagcagtg aaaccagaac cacaaagaag
    15481 gcgacattaa agaacagtcg cgcattctcc agccccgcca tcatcgggaa cacggctagg
    15541 atgattggca ccgcgccgcg taatcccacc cagctgataa acacgcgctc acgcagattg
    15601 aaaccgcgga aagggagcaa tccagcaaat accgaaaggg gacgggcgaa gaatatcatc
    15661 catgccgaca aaatgagcgc cggaatggca ataggcagca gatcgcttgg gttaaccaat
    15721 agccccagca ccaggaacat ggcgatttgc gccagccagg cgaggccgtc gaaattttgc
    15781 aggatgccgt agcggttgcg aatcggccga ttacccagca gaaaaccgca cagataaaca
    15841 gccagaatac cgctgccttc cagcgccgtg gttaaagcaa aaattaaaat accgccgctt
    15901 aatgccagca atggatataa tccggcaggc agggcgatgc gattaatcat ttgcagcagt
    15961 aaataaccac cgccaagacc gatgacaatc ccgaggccaa attgctgcag aatatcgacc
    16021 acgaacatcc aactgacact gctttcatgc tgctggatca ttgcaatcag ggtaatcgtc
    16081 agaaagaccg ccattggatc attactgccg gattcaattt ccagcgtcga gccaacacgt
    16141 tcgttaagcc ccttaccacc cagcagagaa aagaccgctg cagcatcggt ggagccgacg
    16201 atagcgccga ttaataagcc ttcaatcaaa tcaagattaa acagccacgc cgccatcatg
    16261 ccggttaaac cagaggtgat aagtacgccc agcgtcgcca gcgacagcgc cggtcctaac
    16321 gccacacgaa aggaactggc ctgagtgcgc atcccgccgt cgagcaaaat aatcgccagc
    16381 gccaggttac ttaccatgta ggcgaagggg taattatcaa acgggatacc gccgacgcca
    16441 tcgactcccg ccagcatgcc gatcgccaga aaaataacca gaataggaat gccaagacgg
    16501 gaagaaaatg aactaagtaa tatactgctg gtgacaagga tggatcctaa gatgaaaagg
    16561 ctaattattg ttgtggcatc caatggtcgg ttactcctga ttacgctgtc tcttatataa
    16621 accctaccat attagcggca gagacagcgt tttacttagc cctgaagcgt gattttttta
    16681 cattttaaga acaggatgac cgctaatcgt caactgagcg ccttcccggg tgttattcag
    16741 aatggcatgt gcgcccaacg gcagcgtaac cgtgcgttgt tcatgaccaa aatcgaggcc
    16801 ggtaatgagt ggaatcgaca ggcgggaacg caaaaacgcg tacactgact ccaggttgta
    16861 acctgcgtca taatcattgg gcgtgctgcc gctaaagcta ccgagaataa tcgccttctg
    16921 acgtggcaaa attcccgcat gatagagctg caacagcata cgttcgacgc ggaaagggtg
    16981 cacgttaata tcttccagca ccagaatacc gttctcaatt tttggcatcc acggtgtacc
    17041 aatcagtgaa atcagcatcg caagattgcc tccccacaac gtgccttcgg tctgacatgt
    17101 cgggccttcc ccttgccatt caatggtgaa ggtttcattg cgtaacgcca gccagaaatg
    17161 gtgctcggta aaggcgttca gttcatccgc accaaagttt gctaccagca tggggccgct
    17221 aaaggtgatg acattgcctt gcgccagaag accgcactga atggcggtaa aatcgctatg
    17281 tccgcaaatg agtaacgggt catgttgttg gcgagccacc agtgcctgcc agtcaatatc
    17341 tgccagtaaa cgactggcac cgtaaccgcc gcgtacagcc agcacgatgg tgttgggggt
    17401 agtcagtctg gcaagggaat tgagatcttc cagacgctcc gtttccgtac ccgcaaaacg
    17461 ctcacaacga cgggcaataa cctcgacgtt attgacttga tgccccgcgt cggtaaggcg
    17521 ctggatacca cgcagcgcgg cgtgctgttt aatgctgtaa cccgatgggg caattaagtg
    17581 aaacagagac atggcaattc cttgctgaca acagaaacga aatgtatatc atgccgctta
    17641 ggtgtgccgt tgtcacctca acggcgattc caggctataa ggatagaaga agtgaaattg
    17701 agatggtttg cctttttgat tgtgttatta gcgggttgtt catcaaagca tgactatacg
    17761 aacccgccgt ggaacgcgaa agttccggtg caacgtgcga tgcagtggat gccaataagc
    17821 cagaaagccg gtgcagcctg gggcgtcgat ccacaattga tcacggcgat tatcgctatc
    17881 gaatcgggtg gtaatccgaa cgcggtgagt aaatcgaatg ccattggttt gatgcagtta
    17941 aaagcttcaa cctccggacg tgatgtttat cgccgtatgg gctggagtgg tgagccgacg
    18001 accagcgagc tgaaaaatcc agagcgtaat atttcaatgg gggcggctta cctgaatatt
    18061 ctggaaaccg gcccgctggc aggcattgaa gatccgaagg tactgcaata tgcgctggtg
    18121 gtgtcatacg ctaacggggc aggtgcgctg ctacggactt tctcgtcaga tcggaaaaag
    18181 gcgatcagca aaatcaacga tttggatgct gacgagttcc tcgatcacgt agcgcgaaat
    18241 catcctgcgc cgcaggctcc gcgctatatc tacaaacttg agcaggcact ggacgcgatg
    18301 taaatcagtc gcgcactttg tccgcttttt cccgggcttc tcgctcgaga gagaaaataa
    18361 tccgctgtaa ttgccgctcc accgtcgggc tgacgttaag aaaacggaag ctcagacggg
    18421 gagtggtgat ggtttcattc ttgccatcaa tcactttgcg ctcgctggtg gagattaact
    18481 gggcgtcaaa gtgaaaaaca ccccattgcc ccatgttgac ttcaatttga gcgaagcgca
    18541 taccttcttg taattcggca ggctttgctg tttccagtaa tgcgcccatg ccgcctaacg
    18601 acaaatcata caagcggaaa cgtaacgtac tgttatccgc cagtttggtc tggcaaaaat
    18661 aaggcggatg gagtggggcg gagatgcgga aatatcggcg tcgttgtaca aaccataagg
    18721 tgggaggcgg tacggtaata aatgccggaa gctgcaagta ttcactctgc tgtagttgtt
    18781 caacagtaaa ctcgactttc gcaccctgag tttcggcggt aatggtaatg tgctgtgcct
    18841 ttagcacggc gatgttgtct tcggcttgac tgccgaaatc cagcaccagt ttatccgggg
    18901 ttattgccag tattttgctg atcagctgcc cgccattcca actgagacgc aaaggaattg
    18961 cggctttgtg caaatcgcgt aacacgccca ggacggctaa cggattttgt ttcaggaact
    19021 gctcatggta atgactcacg cggaaaaact cctgactgac aaactgtctt tggtttatcg
    19081 gtcatagtta acaaaactta atacaaatgc gtgaatattt tttacatgtt gttcttaaat
    19141 cagccgacaa acgcccagca tcgatcccgc ccttgcctat acttagagcg ttgacgtaag
    19201 catttcttgc gaccgattca tcgaaacgag ggcctgaaca tgggaattat tgcctggatt
    19261 atttttggcc tgatagccgg cattatcggc aagctaatca tgccggggcg tgatggtggt
    19321 ggatttttcc tgacctgtat tctcgggata gtcggtgcgg tggtcggcgg ctggctggcg
    19381 accatgtttg gcattgacgg ctccatcagc ggttttaatt tgcacagctt cctggtggcg
    19441 gtggtgggcg ctatcctggt gctgggcgta ttccgcctcc tgcgaagaga ataagatttt
    19501 cataaggcgg atagcgatac agatgccgct atccgctttc acatcagaac gtatactcga
    19561 cacctgccca gaaattgcga ccttcttcaa taaacccttc actgtaggca tattgggtat
    19621 caaacaggtt attgaccgac gcattgacgc tgaagccatg acctaaggtg taatcggctc
    19681 gaatgtgggt caccgcaaaa ccggcggctt tttgtgaacc gtcactgttg ctgtagctgg
    19741 aggaacgcgc ctcttccgac agcgttacgc ttaacggctc ccatggtttg agtgtcatcc
    19801 atgcggtcat tgtctgcgtt ggaagatcgg ttatcttgcc gatgtctttg cgtttggcgt
    19861 cagcgtggat cagagcgtag ctcaatccta catccagtat attgctgatt ttccccttaa
    19921 taccggcatc cagaccgctg taatccaccg tgccgctgtt ctgattttga atggtatcgg
    19981 cattgatatt gtgcgagagg atggcatcac tcacccggtt atagtaaacg ctgacctcaa
    20041 atccccagtc gcgcgtgaag gcaccattcc aggttaaatc caccccgcga gcgcgttccg
    20101 gcttgagctg cgggttaaca atcgctatct ggttatacgc aggtttggat gtggtatagc
    20161 gttctttcag cgtcggaaag cgtgtgcggt catagtacga aagcgccaga gtgtcttcat
    20221 tggcaaagtg gtatttcccc atcacctgcc agttaaaagc tgactgattg ttgtcgtcat
    20281 aatgggtgat gctgccatct ttctcgtgtt ttttcgcttc tacgctatcg cgccagtcat
    20341 agctgattcc cgccacgaca tcgacattat cagcagcagc ccattgatat tcactggcga
    20401 gcgaccaggt acgatcttca tagcgatcgt aagcggcgtg cggcgcacct ttttcccggt
    20461 gtacgtcatc tttccagtta acggcaaacg acagcagatc gttttcacgc acatcggctg
    20521 ccagttgtaa tccggcaccg tcgctgtaat cggaataatg gctgtagctg ccttttttat
    20581 ttttcaaatc agccagcgag ttgtacatca tcagcgtatt ttcaaaggtg tcgcgataca
    20641 gccgactttt cagggtaaaa cggtcgttta gttgggtcgc tccctgataa taaaaacttt
    20701 ctttgtcata ctctggccac tgccagtagc gtgatttttg accacaattt ccgctgtatg
    20761 gcgggttatc tttttcacca tcctgcttaa tgtaagtcag cgtgtattca tcgttttcgc
    20821 gcggtgtaaa accgagcttc acaatgccgc gtttatcatc agccgatgaa ttaatcatct
    20881 tgccgtgttt gcctgcaata tcattattta caccatgcgg caggccgaga aaatcctgct
    20941 ttagctggct accgctgact tgcaaatacc caagatcgct gctggcggca aatgaagcat
    21001 gcatatcgta ggcattgtcc tgactacggc tccatccctg gcgatatccc agacttgctt
    21061 cttcagggtg gtgatattaa tggctccgcc catctgatta ggcccatgaa gcagcgacga
    21121 ataccctttg gaaacttcga ctgcccccag attgttggtc agaatccgcg ccagatcgag
    21181 gttgccgtca taggggacat aaatgggcac accgtcgaaa tagaccggca cctgccgact
    21241 atcaaagcca cgaactttta cctgttcttc gttgcggctg cctgactttt gcagcaccac
    21301 gccggggacg acacttaatg cctgagcgac attctgttta tccaaggcct tcatggtggg
    21361 ttgatcgaga acggtggtcg tcgtcgatga taccggactg gaccatacgg tcagcgtgtc
    21421 gctctcctga gccgctacgg cctgctgggt aaaggcaagt gataatgctg tgcagagata
    21481 acgttttttg agtctcatag aataaattcc gtttaacaaa gaattaaata gaggctgtgg
    21541 gttgcagact gactttaatg tcagctggtg ccgcgtaata aggcgcggag gtaataaaaa
    21601 ggcgaatgcc gcagtcgagg taatttttga gtgtcgtcag attaattccg ccggtcagag
    21661 ccagcgtgca gtgaggagcc agcgacggcg ctatttgcgc aatttcagtt gcctgttgcg
    21721 gactaaattt gtcgagctga agtacgtctg gttgtgcgcg taacgcggcg atagcctctt
    21781 tcggcgtgtc ggcttcaaca acaattttct tctcgggtgc gtggcgacgt aactgagcga
    21841 ttgcgcctga ccagccctga ttgtcatgaa gaaaatggcg gtggttggca aacaataata
    21901 tggtttccgc acatccggca cgatgaatta gtcctccggc agccagaatt gcctgcgaag
    21961 ccagtaagcg ggtgcccgga attgcttttc gggtgcaggc gatattgccg tcaggataac
    22021 gttcacgaag caatgccagc atttgagcga gataatcaga aacaccgcaa ctccactcca
    22081 gcacattctg gactgccttc catccctgat gaagtgctgc ggcattaccc tgcgcacgga
    22141 ttagccgttg acccgcgttc gcttgtgaac cgtcgctgac cgcgtcatca atagttaatc
    22201 ccagcgtagt taacatctta cacgcgacag aaacgccgct gacgcagccg ccctgacggt
    22261 gaaaaaacgc tatatagcca ggctggtgtc caatatttaa tgctcgcgtg gtcaggtcac
    22321 ctccctggat atcttccagc agtaacgcat cgatttgtgc ctgagaaagg aaaatcatca
    22381 cagtgcctcc tgcggtacgc tatcccacca gatgagcgcc cagtcgcggc tggcaggggc
    22441 gagggcacga gcatcttgtt gttgatacca gtgatataac cgctcacgct catcgtcgtt
    22501 aagcgcgcca agactccagc tgacgttctg ctcaaaacgt tcccaggtgc tgttatcctg
    22561 ttggcaattt tgtccacgga taaaatcaac gtgggcataa attcccatct gatacaggac
    22621 gttgagcgca aagatatagt tgggtaattc gatcacttcc cgaccagccg cccgctgaat
    22681 ggctggagag acgaacgagg tgctgaccag atgagttgta tagacgcgaa gccgtgcctg
    22741 attattcagt ttgctcattg catggcgcat atcagccacc agagtggagc gggaagccac
    22801 ggcaatatcg cagcgtggca ggtcactcca gtcctcttcc caggcgcgct ggatccagtg
    22861 gacattgtca gccttcagtg cggcggcgcg gcgggcggca acgttcagca tcccctgact
    22921 gtaatcaacg ccataaaccg tagtgagttt gtcagccagc gccagcgata cggtgcctgg
    22981 tccgcagccc atatcgaata aggtctgagc gccttgcagg tcaattttag cgatcagttg
    23041 ttgcaggtag ctgtccgttg gcgaggcgca attttccgcc attttctccg cccgttgatc
    23101 ccagtgatcg ggcgtttttt ctgtgcggtg agccagtttg agttgctgca gataaaggtc
    23161 tgcaaaatca atatcatcaa tgagcatgat tgttccttag cggctaattg cagagaggtg
    23221 atgatgaatt tggtcggcag aaacccgata cagggccgcc agtttgttcg ttgttaactg
    23281 ttcggtcggt aagccttgtg ttactcgtcc atctggctct acttggataa tgctgtcggc
    23341 aatggcgttg gcatggagcg tatggtgagt cgacattaac attgtcatgc cgttattttt
    23401 gagctgcgcc agcgtgtcca gaagttggat ctgatgaccg aaatcgaggc tggaggcggg
    23461 ctcatccagt aacaacaagc gcggttgttg gacgagtgca cgggcgatta aagccagctg
    23521 acgttcaccg ccgctaagcg tattccagcg tcgtgtcgcc agatgcaaaa tgcccagctt
    23581 ttccagttgc tccgtggctt tcaggcgttc ttgcttaccg ggaacggaaa aagcgcctat
    23641 ggtgggggcc aggcccatca acaccatatc cagcacactg aaagcaaaaa taccgtcgtg
    23701 cgcctgcggc acccaggcaa tagcctgtgc gcgctggcga tggctgagtg tctgcacaga
    23761 gaggctatcc agtaggatct cgccttttaa taacggtata acgccaagaa tggagcgcat
    23821 gagtgtggtt ttgccgcaac cattcgcgcc aagcagacaa caaattgttc cttgcgacag
    23881 ggtgaaggac acgtcgcgta gcactggatg gcgggagtaa cccagagacg catgacgaac
    23941 ggcaagcaaa gtcactggcg acctccgcgt aacaacaaaa gcaggaaaaa cggtgcgcca
    24001 acaaaagcgg tcagaatgcc aagtgggatt tctgttgttc caatgctgcg cgccagcgta
    24061 tcggtaagca ggagcagaat agcgccagta cacattgcca taggcaacag ctgctgatgg
    24121 ttatgaccgg tcaacaagcg ggcaatgtgc ggaacgacca gaccgatcca accaattatg
    24181 cccgctattg ctaccgtgct ggcggtgatt agtgtggcgc agacaatcaa tccaaagcgc
    24241 agacgcgtca cgttcagtcc cagcgaacgg gcttcgtcgt cggaaagcgt gagtaaattc
    24301 atgcgccagc ggagaaaaaa caggggcaag gagcctatga gaatgatcgg tgcagcatag
    24361 cagagatcgc gtaaggtcac ggtggacagt ccccccagca atcagaaggt aattgacggt
    24421 aactgggtgt aaggatcggc cagcgtttta attagcgata tccccgctcc gcacaacgtt
    24481 cctaacgcaa tgcctaccag caccagggtt aagatcggat cgtggcgggt cacccgacgc
    24541 gtaatcaacc agacgcccgc gaccaccatc agcccgccgc aaaatgcgta cagctggatg
    24601 taaacaatgg gtaatcccca caggattgcc gcgcaggcac caagtccggc accggcggag
    24661 acgcctaaaa tatccggtga aactaaggga ttacgcaaca ttccctgata ggtggttccg
    24721 gctcctgcca gcgctgcgcc cagtaacagc gcagcaagaa tacgcggcac gcggatttgc
    24781 cagaagacaa tttgttcctg cacagactca ttttcttgac gaagaattag cgccagcagt
    24841 tgctgcatat cgagatgata agccccactt acggcggcaa aagtgatact gatagctgcc
    24901 agcagcaaaa tacatccgtt tacgattctc attagctcgc caccaatttt tgatagtcgg
    24961 catcactgag tgaacaatgc cagaacagtt gggcgtaatg ctgcatgtca cttttaaact
    25021 ggcgattgat ggcgggatcc agccaggcgt gaagtctgcg taatcccaga agacggttga
    25081 ttcccggcgg ggcatcgagc cagccaaagg gcaagccact taaaaataag atgcgttgtt
    25141 ccgcaacggc tttcacgccc tgccagagag gatcacgacg aataaaatct gcagtaacgg
    25201 cctcctgaac cagaataata tccggctgcc agcgcaggag attttccatg gaaacctgtg
    25261 tcaggccgtg gcgatcggct atttgcgcga cgttgtgcaa acctaataat tccgccgcct
    25321 cggtatgcaa cgaaccctgt aagcccgttt ccagccctcg gggaccgcgc gcagcataaa
    25381 agcggaggtt agcggcgggt gaggtggcga atgcctgagc ttcaccaacg aagtggctgg
    25441 cgagattggc ttgttctgcg gcgcggtgct cttcgcctaa cgttttgcca agcgttgtta
    25501 actgttctgc tgattgcgcc agttttccgt taagcaataa ccaggggatt tgtgtctgtt
    25561 cgctaacctg ccgtgcctgg gagatcaagg tttcatccgt attgccgcaa tcaacgacca
    25621 aatcgggatg taacgccata agcccttcca gcgatagtgt gctggcgcgc ccggcgagtc
    25681 gtcccagcct gggtaactgg cgaatgtgct ctggcaatgg aattaatgcc tgacgggcaa
    25741 aatcaaaaga ggaaaaaccg accatttttt ctggcgcgac agccagcaag agcaaatcgg
    25801 cgggcgcgcc tgcgctgacg atgcgtgtaa tgttttgggc ttcgggaaga gattgcgaag
    25861 caaaacgatt aactgtttgt gaaaaggacg gtagagactg caggagcagg gtagatgcaa
    25921 gtgcctgcgc aaacgtccgt ctggttatgg gcatggtgaa catcctgaaa agtaaaatcg
    25981 atatatactt tcatatatat cgataaaaat catgttcaat atcaaagttt agctattgaa
    26041 aacatgaaga tgtgacgact tccgcaagcc tgttgaccct ctctatcatt aaaaatgtat
    26101 ttatcaggtg gctaatatta tttaaatcat ttaaggctaa attatttaaa tatgaatgac
    26161 attattgttt ttttatcgtc cacggttacg cgacgttgaa gttacttgtt ttataatgag
    26221 cgatttttga tatctcgccg agaaaattat cagcattctc tcgtttcagt gtcgtgaata
    26281 tactgttaac ggaggtgaaa agaatgctgg gggttataca aaacctatgt tgttgtcgtc
    26341 cggattcacc gcttgctgat agtaagcagc gtaaccagaa gttaccgcaa caagactttg
    26401 aggattttaa gaacgacgtt attaatgtca ttaacaacaa tgaagatatt tattttctta
    26461 gagagagtaa tttactgaat gcgtttattc ggacgtgcaa aaaaagagat gataacagtt
    26521 acggaaatat gatatttacg ctttattttg ataacgatat ctatgggaac agaattatca
    26581 aagcatcgct gaacggcgga gagttgcagt tattaagcac agggtgtagc aatgataaca
    26641 ttctccat
//
LOCUS       DAJTPG010000052        25864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTPG010000052 DAJTPG010000000
VERSION     DAJTPG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(18..666)
                     /locus_tag="OO230_002254"
                     /pseudo
     CDS             complement(18..666)
                     /locus_tag="OO230_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            731..1882
                     /locus_tag="OO230_002255"
                     /pseudo
     CDS             731..1882
                     /locus_tag="OO230_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1912..2340)
                     /locus_tag="OO230_002256"
                     /pseudo
     CDS             complement(1912..2340)
                     /locus_tag="OO230_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2855..3898)
                     /locus_tag="OO230_002257"
     CDS             complement(2855..3898)
                     /locus_tag="OO230_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5691998.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(4310..5521)
                     /locus_tag="OO230_002258"
     CDS             complement(4310..5521)
                     /locus_tag="OO230_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5691999.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            complement(5793..5887)
                     /locus_tag="OO230_002259"
     tRNA            complement(5793..5887)
                     /locus_tag="OO230_002259"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5851..5853),aa:Sec,seq:tca)
     gene            6180..7563
                     /locus_tag="OO230_002260"
                     /pseudo
     CDS             6180..7563
                     /locus_tag="OO230_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            7573..9891
                     /gene="yicI"
                     /locus_tag="OO230_002261"
     CDS             7573..9891
                     /gene="yicI"
                     /locus_tag="OO230_002261"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5692000.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(9944..11653)
                     /locus_tag="OO230_002262"
     CDS             complement(9944..11653)
                     /locus_tag="OO230_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5692001.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(11774..13165)
                     /gene="xanP"
                     /locus_tag="OO230_002263"
     CDS             complement(11774..13165)
                     /gene="xanP"
                     /locus_tag="OO230_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5692002.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            13445..14650
                     /gene="gltS"
                     /locus_tag="OO230_002264"
     CDS             13445..14650
                     /gene="gltS"
                     /locus_tag="OO230_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5692003.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(14684..16765)
                     /gene="recG"
                     /locus_tag="OO230_002265"
     CDS             complement(14684..16765)
                     /gene="recG"
                     /locus_tag="OO230_002265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5692004.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(16771..17460)
                     /gene="trmH"
                     /locus_tag="OO230_002266"
     CDS             complement(16771..17460)
                     /gene="trmH"
                     /locus_tag="OO230_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5692005.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(17467..19575)
                     /gene="spoT"
                     /locus_tag="OO230_002267"
     CDS             complement(17467..19575)
                     /gene="spoT"
                     /locus_tag="OO230_002267"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5692006.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(19594..19869)
                     /gene="rpoZ"
                     /locus_tag="OO230_002268"
     CDS             complement(19594..19869)
                     /gene="rpoZ"
                     /locus_tag="OO230_002268"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5692007.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            complement(19924..20547)
                     /gene="gmk"
                     /locus_tag="OO230_002269"
     CDS             complement(19924..20547)
                     /gene="gmk"
                     /locus_tag="OO230_002269"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5692008.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            20805..22487
                     /gene="ligB"
                     /locus_tag="OO230_002270"
     CDS             20805..22487
                     /gene="ligB"
                     /locus_tag="OO230_002270"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5692009.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(22484..23101)
                     /locus_tag="OO230_002271"
     CDS             complement(22484..23101)
                     /locus_tag="OO230_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5692010.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(23392..24216)
                     /gene="dinD"
                     /locus_tag="OO230_002272"
     CDS             complement(23392..24216)
                     /gene="dinD"
                     /locus_tag="OO230_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5692011.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(24437..25300)
                     /locus_tag="OO230_002273"
     CDS             complement(24437..25300)
                     /locus_tag="OO230_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5692012.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            25427..>25864
                     /gene="rph"
                     /locus_tag="OO230_002274"
     CDS             25427..>25864
                     /gene="rph"
                     /locus_tag="OO230_002274"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5692013.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVE"
BASE COUNT         6176 a         6362 c         6718 g         6608 t
ORIGIN      
        1 acgcagtcag agtacactta ctttttatta tttcctgcaa tggcgattac ggctattgac
       61 agtaatgaag ttattagtga accaagacct aaagtagttc cgggaccaac accgttgtcg
      121 cttacggtta ctgacaggaa actgattgta ccagcgctta ttgtttgagt aaaacctgta
      181 atagatgcgg caaatcctgt ttttgaacca tttatttgta atgcgataaa attggctatt
      241 gggtaataaa aaccataggt tggataattt ttcaatcttt gataattgat ttccgattat
      301 gaaaaataaa actgagaagg agaaaataat tgagtaacta aaagtactca ggccataagt
      361 atgcatgaaa attgatggtg atattgatat gtagcaaaat agagaactga gtattaatga
      421 gacaagtata gtgccgcaga taaactcttt ggtcacaaag gatgcactgt tttgacatat
      481 tactttctca tttgcatgag ttatctttgg tgataataac attattataa tactgaacag
      541 catcataaca agaaatataa cattccagtt ataatattca ataatcaatg cgcgaggtgc
      601 tactaatggt attactgcgc ttacagatga tatatcagaa agcgcccgtt ttagttttaa
      661 tggagggtga tcttacccac caatagtgga cacaggacta agtgagtaaa ctctcaacca
      721 gaggtgactc atgacaaaac cagtatcaat cagcaagaag ccccgtaaac aacatacgcc
      781 tgaatttcgt aacgaagccc tgaaactcgc tgaacgcatc ggtgtggccg ccgcagcccg
      841 tgaactcagc ctgtatgaat ctcagcttta tgcctggcgc agtaaacagc agcaacaaat
      901 gagttcgtca gagcgcgaaa gcgaactggc cgctgaaaat gtccgcctta aacgacaact
      961 ggcggagcag gctgaggaac tgtccatcct ccaaaaggcc gcgacatact tcgcgaagcg
     1021 cctgaaatga agtatgtctt tatcgaaaat catcgggcag agttcagcat caaagcgatg
     1081 tgtcgtgtac ttcgggttgc ccgcagcggc tggtatgtct ggctcaggcg tcgtcaccag
     1141 atgagcctgc gccaacagtt tcggctcacc tgcgatgccg ctgtccataa ggcattcttt
     1201 gaggcaaagc agcgatacgg tgctccccgc cttgctgacg aactgccgga gttcaatatt
     1261 aaaaccattg ccgccagcct gcgtcgtcag gggctgcggg cgaaagccag ccggaagttc
     1321 agcccggtca gctaccgtgc acatggcctg cccgtattgg agaatctgct ggagcaggac
     1381 ttcagcgcca gtggcccgaa accagaagtg ggcgggtgac atcacgtact tgcgtaccga
     1441 tgagggctgg ttgtatctcg cagtagtcat cgacctgtgg tcacgcgccg tcattggctg
     1501 gtcgatgtca ccgcgaatga cagcacaact ggcctgtgat gcactgcaaa tggcgttgtg
     1561 gcggagaaga cgcccggaaa gcgtcattgt tcatacggac cgtggtggtc aatactgttc
     1621 aggggattat caggcaatgc tgaagcgaca caacctgcgt ggcagtatga gtgcgaaagg
     1681 ctactgttat gacaatgcct gtgtggaaag cttctttcat tcgctgaagg tggaatgtat
     1741 ccacggggaa cgctttagca gccgggaaat aatgcgggca acggtgttta attatatcga
     1801 gtgtgattac aatcgctggc gtcgtcacag tgcctgtggc ggtctcagcc cggaacaatt
     1861 tgaaaaccat aatctcgctt agggccgtgt ccacattacg tgggtaggat cagtatgggt
     1921 cgtcttgtat ttgtcatact gcccaatctc accaatcgaa aatgctcctt ttggcaaagg
     1981 ttcaagcagg tctctgggaa tggaggtcgc tggcgtcgga tataaaaaac cctcacggct
     2041 tagcaaggct ggcgtcacta tcggtgtaac aatatcaggc gtgcctgcgg aacggtcctt
     2101 accatgctgc agtaaccgga aataaacttt tgggcgtaag atgacattga cagtttcgac
     2161 atcgtctttt tctcccttaa aaaatttatt gacaatcgct tcgtcaagac gtcccgccgc
     2221 tatcccatgc cagtgagtga aattttgggc gtctttccgt tcaaaacagc cctttcctga
     2281 ctcagcatct gcaagcgagt tgcgccagta tgaggcaaac cttaaagcat tttcatccat
     2341 taccagtcct gtgactcaat gaaatccatt gatttatcga atataaaacc accgtatgga
     2401 caactattgt ccatattaaa aaagttggat gcaatgtttt gtttataatt cgatatcaaa
     2461 tgtactggtt gtacgaggtt cagaacacaa gcacattcaa cagtacgtca ggactattga
     2521 tttcataaca gcgaatacaa ttttttactg tttataatgg ctgcctgtgt tggacgggga
     2581 taccagttca accgaaaacg ccagaggagg ggattacccg ctggcagggt aaatctgtgg
     2641 tttttgatgc cagctataac gtgtttgacg ataaagtatt acgaaagaac tttaatgagt
     2701 tcatggagta attcgttaaa gtaatttaca acgctatcct tttgcaaagg aaactttcag
     2761 aaaataaccc cagggtaatc acctggggaa ggaacacagt gttattattg gataataaat
     2821 tattacttgt tttaaagtta cactaatgca atatctactg atactggatg cttgaatgtt
     2881 tcatccctgc tgcggaaaag ggcaatatcc agggccagat atattcaccg ttcatatttc
     2941 tgcatttctg tacaatttcc tcagaaagtg tcttgtcaat tcttttccac aggtgctcct
     3001 gctgtcttaa aatactttcg catatttcga aatcatcttc ctgaatcagc atttcggcgc
     3061 atttaatgat ttctgatcgt tcatgcagca cggaggaatg gttatagagc cagtttacaa
     3121 acagcaatgc ttccggatat tgtccccata atggtgagta tgttgcatag taatcttctg
     3181 ccgtatccag agagccggag aagtcatgcc acacagcaag tggaaatgga tgcagatttc
     3241 cgcacagcat ttctgcgatg taagtgtttg tggcaaagcc atggcgaaaa gctgtggcgg
     3301 ccttaacatg ttccccgttc agaaaatgca ggagaccgag ttcataccag taaggtgaaa
     3361 attcatcagc atgctctttc agtacactga aggctcgctc atgatcgccg gtgcgtaaca
     3421 gctctgaacc caacaaccag cgagccccct gattatcttc aggattgtat gccagtattt
     3481 tatcagtgag cataaccgca tcctgatggc gctgtaagtg cacatttgcg agaattgctg
     3541 cataaagcgc gcgcagataa ggccggtttt cgggatgcat ccagattatt tcaccacaga
     3601 aactttccgg gatgatacga ttacctgccg ccagtccttt aagagcggcg ttcagtgcct
     3661 tacgcggggc attctgttcc agaaatgcat atgccagatg agcgtgtata tcaataaagt
     3721 ccggctcccg ttgagcaagg cgtcggagtt tgttgatgta actcgtctcg ctgatttcat
     3781 cagcgtcgta tgcatcaagc acttcttcaa actcccccag caatgggcca aaccaggtat
     3841 ccggaaatac gaaataaccc ttgttatcgt gcacttcaaa gcataatcta tcgttcattt
     3901 tttgagcctg taaaacatgt ggtgatatag tctgagagca taaaagttga gattctgtgt
     3961 atcaagtcct gtcttggcac cgaatgaata ttgcttacga agccccttca tgctttcctt
     4021 gagaacaata attttatctt tatgtgaatc tcatttttta taatgatgtc agatgattga
     4081 aatgctaaat tatatctgaa tcaactattc ctttgctaaa ggtatcgaaa taactgcaag
     4141 gctgtatttt gtaattgcca atttaaaaat gtcaatttgt ttttaattat gtttttgtaa
     4201 taagtttgaa gacgcaaaaa aataatataa taacaaaaat cctccagcct tgtaggccag
     4261 aggataaaat acttaattga ggaaaacatc agggaaaagt atttctgact taactttccc
     4321 ggctggagag tgaatcaatg taatcagcgt accattgcat catttcacgg cggccatcga
     4381 gatacagggc atgattatag gtacccctga tggaattttt atccacatga gctaactgca
     4441 tttcaatcca ggcggaatta aagccttgtt catgcagaat tgtactcatg gtgtgtctga
     4501 aaccgtgccc tgtgagtctt cccttatatc caagtaattc aattacttgg ttaatgcttt
     4561 ccttactaat tggtttgcgg ggatcatttc taccgataaa gactagggga tgatgttttg
     4621 agattggttc aagctgctta aatagcataa tgacctgctc agagagcggc acaatatgag
     4681 gtcgcttcat cttcattact tctgccggga tctcccacaa ttttgtctca aaatcgatat
     4741 cttcccagcg tgcaaaacgc agttcttgtg ttcgtacgcc ggttaacata atgatctgag
     4801 tagctgtctt agtgattata cttccggtat aacccgccag atcgttgagg aagtggggaa
     4861 gttcattagc agtaagaaag ggaaaatgta cttttttcgg tgtagctaaa gcactggcaa
     4921 gatcgggggc tgggttatag tctgcccggc cagtaacaat tgcatagcgg aacacctcac
     4981 cacagcgctg gcggaccttg cgcattttct ccagagctcc acgtttttcc attttacgca
     5041 gtgcttccag taacttcatt ggtttgattt cggcaatagg gcgcttgcca atgtagggaa
     5101 aaatgtcttt ttcaaaggta tcaatgattt catcacgata gcgtaatgac caccgatcag
     5161 cttttaactg gtgccattcg cgagcaatag cctcgaacgt atttttcaga ctcatttgct
     5221 gcgcgatttt ttcttccttt tttgcctccc ctggatcgcc tccagcagcg aggagtttcc
     5281 tggcttcact gcgtttctcg cgagcatcgg ctagcgttac atcggggtat acgccaatag
     5341 aaagcatctt ttctttacca gcaaagcggt acttcatgcg ccagtattta gaaccattag
     5401 tgttaatcag tagatacata ccgccgccat cagcaagctt atagggtttg tctttgggtt
     5461 ttgcggtttt cactttgata tcgttaatgc catgacatcc tccggctttt gatgaattca
     5521 tgttcctgag ggggatttct gccagtaact aacctgatac ccccaataca ccccttgtat
     5581 aacaaagata ttgttaaaag ccgttgttat gtatcacatt tttcctatta tgggggtgct
     5641 tgtatttcaa taggaaataa caccccctaa agtaccccca aaagaaactt gatacggggt
     5701 gaagtaggtt gacctcagga tacaaataaa tactgatata caagggatta gaagagattt
     5761 attgacattg ggagagttca gaagaggtaa actggcggaa gatcacagga gtcgaacctg
     5821 cccgggaccg ctggcggccc caactggatt tgaagtccag ccgcctcacc ggagacgacg
     5881 atcttccgcg cctcgattgc tacatggagg cggggcgcat tatagctact tccttgagtt
     5941 tctacatccc ccagatcagt tttttccgcc taataaacgc agcgtattat cacgaaagca
     6001 ttataattta tataaaaccc atattttaca atgatatatg agcggaatta acgctaccac
     6061 gatcacataa aacaagaatc ataaattaat aaccagatat cggaatattc gctctcacag
     6121 ggatggttta caaaatgcgt tatcggtctc agcaccccta tagcatcaag gaaaagcaga
     6181 tgaggagtga agtgttgtcc gttaaagaga aaattggtta tggcatggga gacgccgcca
     6241 gccacattat tttcgataac gtaatgttat atatgatgtt cttttatacc gatatttttg
     6301 gcattcctgc cggatttgtc ggaaccatgt ttttggtcgc tcgtgcactg gatgcgattt
     6361 ccgatccttg catggggttg ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc
     6421 cgtgggtact gtttggcgca ctgccattcg ggatcgtctg tgtactggcc tatagcacgc
     6481 cagatctcag tatgaacggc aaaatgatct atgcagcaat tacttacacc ctaacttacc
     6541 ttactttata ccgtcgtcaa tatcccttac tgcgcattgg gtggtgtaat caccaatgac
     6601 ccgactcagc gtatctcgct gcaatcctgg cgttttgtgc tggcgaccgc gggaggcatg
     6661 ctttctactg ttctgatgat gccactggtt aatttaattg gcggtgataa taaaccactc
     6721 ggtttccagg gcggtatcgt ggtcctttcc gtggtggcat tcatgatgct ggcattttgt
     6781 ttcttcacca ctaaagaacg cgttgaagca ccacctacaa caacgtctat gcgggaagat
     6841 ttacgtgata tctggcaaaa cgaccagtgg cggattgtcg gtttactaac cattttcaat
     6901 atcctggcgg tgtgcgtacg cggtggggcg atgatgtatt acgtcacatg gattttgggc
     6961 acgccggaag tgtttgtcgc ttttctcacc acttattgcg tgggtaacct gattggttcc
     7021 gcactggcaa aacctctgac cgactggaaa tgtaaagtca ctatcttctg gtggacgaac
     7081 gccctgctgg cagtgattag cctcgcgatg ttctttgttc ccatgcaggc cagcatcact
     7141 atgtttgtct tcatcttcgt gattggtgtg ttgcatcaac tggtgacacc tatccagtgg
     7201 gtaatgatgt tcgataccgt cgactacggc gagtggtgca atggtaaacg cctgaccggg
     7261 atcagttttg ctggcacgct gtttgtgctc aaactggggt tggccttcgg cggcgctctt
     7321 atcggctgga tgctggctta tggcggatat gatgcggcag aaaaagcgca gaacagcgcc
     7381 acgattagca tcattattgc gctattcacg attgttccgg cgatctgtta tttgcggagc
     7441 gcgattatcg ctaaacgcta ctactcactc acgacgcaca atctgaaaac cgttatggaa
     7501 cagctggctc agggtaaacg ccgttgccag caacaattca cctctcaaga agtgcagaac
     7561 taaggaacgg caatgaaaat tagcgatgga aactggttga ttcaacctgg cctcaatttg
     7621 attcacccgc ttcaggtatt cgaggttgaa cagcaggata atgaaatggt ggtctatgct
     7681 gccccccgtg atgtgcgtga acgtacctgg cagcttgata cgcctttatt tacgctgcgc
     7741 tttttctccc cacaggaagg tattgtcggt gtgcggattg agcattttca gggggcgctg
     7801 aataacggtc ctcattatcc gctcaatatt ttgcaggacg tgaaggtcac aatcgaaaac
     7861 acagaacgtt atgctgagtt taaaagtggc aacttaagcg cgcgtgtcag caaaggtgag
     7921 ttctggtcac tggattttct gcgcaacggc gaacgtatta ccggtagtca ggtgaaaaat
     7981 aatggctacg tgcaggacac gaataatcaa cgcaattata tgtttgagcg gcttgatctt
     8041 ggcgttggcg aaacagttta cggtctggga gagcgcttta ctgccctggt gcgcaatggc
     8101 cagacggtag agacctggaa ccgggacggc ggcacaagta ctgaacaggc gtataaaaat
     8161 atcccgttct acatgactaa ccgtggttat ggggtactgg tcaatcatcc ccagtgtgtc
     8221 tcttttgaag tgggatcgga gaaagtctcc aaagtgcagt tcagcgttga gagtgaatat
     8281 ctcgaatact ttgttatcga cggcccgacg ccgaaagcgg tacttgatcg ttatacccgc
     8341 tttactggtc gtccggcgct gccgcccccg tggtccttcg gcctgtggct aaccacttca
     8401 tttaccacca actacgacga agcgacggta aacagcttta tcgatggtat ggcggaacgc
     8461 aatctgccgc tgcatgtttt ccactttgac tgtttctgga tgaaagcctt ccagtggtgc
     8521 gattttgagt gggacccgct gactttccct gacccggaag ggatgatccg ccgcctgaaa
     8581 gcgaaagggc tgaaaatctg cgtctggatt aacccctata tcggtcaaaa atcccccgtc
     8641 tttaaagagt tacaagagaa aggctattta ctcaaacgcc cggacggttc gctatggcag
     8701 tgggataaat ggcagccagg tctggcgatt tatgacttta ccaatccgga tgcctgcaaa
     8761 tggtacgccg acaaactgaa aggtctggtc gcgatgggcg ttgattgctt taagaccgac
     8821 tttggcgaac gtatcccaat tgatgttcag tggtttgacg gttccgattc gcagaaaatg
     8881 cataaccatt atgcgtacat ctacaacgaa ctggtgtgga acgtgctcaa ggacaccgtt
     8941 ggtgaggaag aagctgtctt gtttgcccgc tcggcctccg tcggtgcgca gaaattcccg
     9001 gtacactggg gtggcgattg ttacgctaac tacgaatcaa tggcggaaag cctgcgcggt
     9061 ggtttgtcta ttggcctttc aggttttggc ttctggagcc acgatatcgg cggctttgaa
     9121 aataccgctc cggcgcacgt ttacaaacgc tggtgcgcgt ttggtttgct ctccagccat
     9181 agccgtttac acggtagcaa atcttatcgt gtgccgtggg cctacgatga tgagtcctgt
     9241 gatgtggtgc gcttcttcac gcaactgaaa tgccgcatga tgccgtatct gtatcgtgaa
     9301 gctgcgcgtg cgaacgcgcg gggtacgccg atgatgcggg ccatgatgat ggagttcccg
     9361 gacgatccgg cttgtgatta ccttgaccgt caatacatgt taggcgacaa cgtgatggtt
     9421 gcgccggtgt tcactgaagc gggcgatgtg cagttctacc tgccggaagg tcgctggaca
     9481 cacctgtggc acaacgatga actcgacggt agtcgctggc ataaacagca gcacggcttc
     9541 ctgagtctgc ccgtttatgt gcgtgataac actctactgg cgctgggcaa caacgatcaa
     9601 cgtcccgatt acgtgtggca cgaaggcacg gcattccacc tctttaatct gcaagacggg
     9661 catgaagccg tctgtgaagt gcctgctgct gacggatcgg tgatctttac tttaaaagca
     9721 gcacgtactg gcaacacgat tactgtgact ggtgcgggcg aggcgaagaa ctggacgctg
     9781 tgcttgcgca atgttgtgaa agtaaatggt ctgcaaggcg gttcgcaggc tgaaagtgag
     9841 caggggctgg tggtgacgcc tcaagggaat gcgctgacaa ttacgttgta atctattgac
     9901 ggaatgccgg gtacgtctga gtcgtacccg gcgagattga tgtttaccgc accggctccg
     9961 tcgatgtaac ttcaccgctg gaaacgacgc ccgcattcat ggtttgagtc acttgctgct
    10021 ttgcggcgtt cacggcatgc aattgcccgc taaccgtagg tttcagcggg gcattggcct
    10081 gaatatctcc actggcggta agctgaatat tgccgtcgcc agtcaacggt aattcaggcc
    10141 atccccattg ttgcaaaata ttcaccggca caccgcgtcc attcaggctg atatgggtct
    10201 gacgttgtgg cgtttgtgaa acacttgcgg tggcttccag aatgcctttt tcagtaaatg
    10261 cactcagttc gctgatattc accgtgctgc tgttggcggt cagcgccagc gacgggcgac
    10321 gaacatcaac acgattgaat gtcgcggcgg cggcattcag attcgccgag ccactccaga
    10381 cgccccattt atgatcggta accagcgtca ggttggcacc gtaaccatcg agcgcggtga
    10441 gctgccacgg gaagtcaggg tcgatatcaa tgatcagatt gcggctggcg ctaaatctct
    10501 tcagttgcag gctgtttaac caaccgggtg tcgtttccat ccacaactgt tgccagtttt
    10561 tcggcaaggt atattccagc ccggcaattg ccgcatcatc aaggatcaac gttttaccgt
    10621 cacgcagcca gttccctgac gttctgacca tacccccttc ccagcggctg gtgaactggc
    10681 gcagcgctac gccctgcggg gaaaattccg cgtttataat cgggtcaaat aaatgcagcg
    10741 aaccataaat gaactcgctg gcgttcatcg acagtttgcc ttcctgtgtc tgccagtcat
    10801 ctttactgaa ggtcatgttg cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac
    10861 cttgcagacg agcatcgatg acttccaagc gaccaatctg caacgacggg acagagcgta
    10921 atggcgcaaa gaagtcggtt agcgattttt cgctttgtag acggatatcc gccatgcgca
    10981 gattttccac ttgccagctg ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc
    11041 gggcgatgtc ggcacccagg ttagtcagcg taacgcgatc gttatcaata ctgccttcaa
    11101 tcagtacatt ggtggcagga acatcattga gcgaaagcga tccggcacta aactgaatct
    11161 gcgccttcgt acccagcact ttaccggctt ctggtgacca cggaaccacg ccgccattta
    11221 cccgctgcgc gctcagtttc cattcgctat tcgggctatt aaacgccata tcacgcagtt
    11281 gcagacgatc ggctttgaac ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt
    11341 tttccagcag gatggtatcg acatggcgtg gttcggtcag ttggcgactg cttagcgcaa
    11401 tgtcgacact ttttgccacc aggggcgcgg gctgaccatc acgaccaaac gtgacattct
    11461 ccagcacgat atgagatggc gcggaaaaac ggtgatccat cgccccgaag gccagatgat
    11521 agtcgctatt ctcggaaacc catgcgctga tatgttctgc tccccagcgg gtttgcagaa
    11581 gaaaatagag gccagcgatc accactaaca gagcgatgag aatgtagaga agcagcttcc
    11641 caataaattt catggtcttc catcccgtga aatgcacata agggagttat gcacgattta
    11701 cgcgcaatcc tcaaggcggg aatggtgaaa gaggtgagac tgcggcggta attatcttac
    11761 cgccgccagt gaattactgt ttttctggtg ggaaaatcag attcagcacg atggcagtaa
    11821 taccgcccgc ggcgatcccg gaggagagca ggtttttcag ccattcaggg gcaaactgca
    11881 aaatcagcgg ctgctgagac acgcccagac caaccgccag cgacagcgcg ataatcagaa
    11941 tcgcccgacg gttcagcggc tcacgagaaa cgatacgcac accggaggcg gcgatggtgc
    12001 caaacattac aagcgttgcg ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc
    12061 tcactgccgg gaacagaccc agcacgatca acatcagcgc gacgacaaaa ccgacatagc
    12121 ggctggcaac accagtcaac tggatcactc cgttgttttg cccgaagcag gagttcggga
    12181 aggtgttaaa caccgccgaa acaaacgagt tcaggccgtt tgccagcacg ccgcctttca
    12241 ggcgtttcat gtacagcgga ccggacactg gctgttcgga aacgtcagag gtcgccgtga
    12301 tatcgccaat ggtttccagc gaagtgatca taaagaccag catcagcggc agcagcagac
    12361 tccattcaat gccaagaccg taatagagcg gcgttggcac cataatcagt tcttgcgtca
    12421 tcggttcgtt gctttctggc aacatgccca taaaccacgc cagcgcatat ccggccgcca
    12481 tcgcaattac cagtgaggcc acgcgtaagt aagggttacg ttgacggtta agcaggataa
    12541 ttaaggctaa aaccacgcct gccagcagca gatttttcgg tgcgccgaag gtgttatcgc
    12601 tcatggctgc gtaaccgccg ccaatggagg tcagccccac ctgaattagc gacaggccga
    12661 taatcatcac cacaacgcca gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa
    12721 cgcgggagat caccatctcg gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca
    12781 tcatggtagg aacatcagca ccaccggttt tcagcgcggt accgcccata atcagcgggg
    12841 caacaaagtt gaagctggtg ccctgaatag acaacagccc ggagccaacc ggaccccagg
    12901 ccttaatttg aataatcgat gccacaccgg aggcaaacag cgacatacta ataatgtgtt
    12961 gcgtgtcttg tgccggtaaa cccagcgcct ggcagattaa tagcgctggc gtgatcaccg
    13021 caacgaacat cgccagcaga tgctgacagg cggcaaacag ggtttgagga agcggcggac
    13081 gatcttcaag acggtaaatc agttcgctgt tttgagtctg agcaaccggt tgcgcatttt
    13141 ctgactcgag ggtggaaaca gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt
    13201 agctgaccga aagagaaaag caaacgtttg ccacatcctc atctaaatat ttaaatgaga
    13261 taaatgcact tttttatata acttttggta tttttctgct taaaatccat gccatttgtt
    13321 cattaacagc tgagaatttg acccaggaaa gcataactcg atacccgtca tagttcatga
    13381 agttgccact gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat acaaaggagt
    13441 aactatgttt catctcgata ctttagcaac gcttgttgcc gcaacgctga cgttgctgct
    13501 cgggcgtaag ttggtccatt ccgtctcctt tttgaagaaa tacaccatac cggaacctgt
    13561 tgcgggtggt ttgttggtgg cgctggcgct actagtactg aaaaaaagca tgggctggga
    13621 agtcaacttt gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg
    13681 cctgaacgcc aacattgcca gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat
    13741 tgtggttgtt ggtctgttgg tgatgcaaaa tgccattggc attggtatgg ctagcctgtt
    13801 agggcttgat ccgctgatgg ggctgttggc cggttctatt acgctttccg gcggtcacgg
    13861 tacgggcgct gcgtggagta aattgttcat tgaacgttat ggcttcacca atgcgacgga
    13921 agtggcgatg gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg gcggtccggt
    13981 ggcgcgctat ctggtgaaac actccaccac gccgaacggt attccggatg accaggaagt
    14041 cccgacggcg tttgaaaagc cggatgtggg acgcatgatc acctcgttgg tgctgattga
    14101 aactatcgcg ctgattgcta tctgcctgac ggtggggaaa attgttgcgc aacttttggc
    14161 tggcactgct tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg tgattctgag
    14221 caacggtctg tcaataatgg gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg
    14281 taacgtaagc ttgtcgttgt tcctggcgat ggcgttgatg gggctgaaac tgtgggagct
    14341 ggcttcgctg gcgctgccga tgctggcgat tctggtggta cagaccatct tcatggcgtt
    14401 gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc
    14461 tgcgggtcac tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc
    14521 gatcactgaa cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc
    14581 gttctttatc gatatcgtca atgcgctggt gattaagctg tatttaatgt tgccgatttt
    14641 tgccggttaa ccgatgaagc ggcggtcttc tcaccgccgc cttttacgca ttcgagtaac
    14701 gttctgtctc cggcatccag cgttctatca aggcttttgc ctgttgtggg taacgttcgt
    14761 gaatatgtcg cgccaggcgc tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg
    14821 ccactttaaa ttcagcatta cccgtctgac gcgtgcctaa caattcgcca gggccgcgaa
    14881 tctccagatc tttttgcgca atcacaaaac cgtcgttact gtcgcgcagc acttgcaggc
    14941 gaatttgcgc cgttttagaa agcggcgttt tgtagagcag cacgcagtga gaagccaccg
    15001 cgccacgacc tacgcgcccg cgcagctggt gtaactgcgc cagacccaga cgctcagggt
    15061 tttcgataat catcaggctg gcgttaggca catcaacacc gacttcaata acggtagtgg
    15121 caaccagcag atgtagctca ccttgtttaa acgacgccat caccgcctgt ttctcggctg
    15181 gtttcatccg cccgtgtacc aggccaacat tcaattctgg tagcgccagt ttcaactctt
    15241 cccaggtagc ttccgccgcc tgcgcttcca gtaactctga ctcttcaatc aacgtacaaa
    15301 cccagtacgc ctgacgacct tcagttatgc aggcgtggcg cacgcggtca atgatgtcgg
    15361 tacggcgggt atcaggaata gcgaccgtag tcactggcgt gcggcctggc ggcagctcat
    15421 ctatcaccga ggtatcgaga tcggcatacg ccgtcattgc cagcgtgcgg gggatcggcg
    15481 tggcggtcat gatcaactga tgcggatgga agccctgctg ctggcctttc tcccacaatg
    15541 ccagacgctg atgcacgcca aaacgatgct gttcgtcgat aatcaccagc gccaggccgt
    15601 taaactgcac ctgttcctgg aagatggcgt gtgtaccgac aatcatctgc acctgaccgc
    15661 tggcgatggc ttcctgctga gacagtcgcg ctttgccttt ctgcttaccg gcgagccagc
    15721 ccacttcgat accgagcggt gcaaaccagt tgcggaagtt attggcgtgc tgctcggcga
    15781 gtaattcggt tggtgccatc agtgccacct gtttgccgtg ggcaatcgca cgcagcgcgg
    15841 cgagggcggc gaccagcgtt ttaccggaac ctacatcgcc ctgtaccagg cgcatcatcg
    15901 gcacatccag cgccatatca tgctcgatct ccgccactac gcgtgcctgt gcgcccgttg
    15961 gcttgaacgg taaggcggcg aggagtttat ttttcagcgt gtcattggcg ctcagcggct
    16021 gggcatgaaa acgctgtgct ccggcacgta aggctaacat gctgaggttg tgcgccagca
    16081 gttcttccag aatcagacga cgttgcgccg gatgctgccc ggtttccaga tcgctaagct
    16141 gtagcgtcgg tggcgggcgg tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt
    16201 gtgacagttc cggcggcagg agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct
    16261 ggtcggttaa tttacgcagc gtggcctgct ttacgccttc cgttgttgga taaaccggcg
    16321 tgagcgtttc ctgtaattct ggcgtgctga gatcgccctg cacgcggtat tccgggtgga
    16381 tcatctccgc accatattta ccgcgctttg cttcgccata agccagtaca cggcggcccg
    16441 tcgccaggct atttttcatt gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg
    16501 aaccgtcgct gatctggcag gtcatcatcc gccgaccgcc gaaggagata ttgcagttca
    16561 gcacttcgcc ttccaccgtg gcataaacgc ccggcagtag ttctccgatg gggtagagat
    16621 gggtgcgatc ttcgtagcgc aggggaaggt gtaagagtag atcctgcacg gtatgcaggt
    16681 tgattttcgc cagtttgtta ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga
    16741 cagcatctaa caggcgacct ttcatggcac ttaccctgca gcctgcatag tagcccacca
    16801 gtcggcatca gcttcgatct cgccttgctg attgacgtgg ggataaggca ggcctttgcg
    16861 ttttgcgact ttcgccagca ccggatagcc gccttcaaac aacaggcgtt gttgctctgc
    16921 ttccggcaac atgctgtttt cacgcaggta catgcctgca ttttgccgct gacgctgggc
    16981 ttcgtaaaga atgagggctg aggcaacgga aacattcagc gactgcacca tgccgatcat
    17041 cggaatgatg atgtcctgat ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc
    17101 ctgtcccatc aaaatgcagg tcgggcgagt gtaatcaatt tcgcggaaat cgacagcatt
    17161 atcagaaaga tgggttgcca gaatctgcat gccctggcct ttgagatgag cgacggcatc
    17221 gccaatggtg cggtgtgttt tcacctgtac ccagctgtta ctacccgccg ctgccgaagc
    17281 catggtgcgc atgcggctac caggccagac ggcgtgaact tcatgtacgc caacggcatc
    17341 tgcggtacga ataatcgcag aaacgttatg aggtttgtgg acctgctcca tgcagacggt
    17401 cagatcaggc tgccgcctgg cgagcatttc gcagatgcgt gcataacgtg ttgggttcat
    17461 aaaacattaa tttcggtttc gggtgacttt aatcacgtct ggcatcacgc ggattttgcg
    17521 catgatattc gccagatgca cacggtcacg agcggtcaga cgaataaagg cgctgtagac
    17581 gcgaccatct ttctcttccg tattcaaact ttgaatattc gaagtcgtgg tgttaattgc
    17641 cgccgtcagg tttgccagcg cgccctgatg attgaacatc tccaccttga tttcggtgat
    17701 gaactcctgc gccgtctctt tatcccattc cacagccata aacttctctg gctctttctg
    17761 gtagccacgg atattacggc aggattcatg gtggatcacc agacctttac cggggctgac
    17821 gtgggcgata atcgggtcgc caggaatagg gcggcagcat ttcgcaaagg tgatcagtac
    17881 gccatcggca cctttaatgg gcagatgtcc gtggctttgg gttgccggtg gaatggaggc
    17941 gtccccatgt tgcagatttt tcgcgaccac cacgctcatt gcgttaccaa gtccgatttc
    18001 tgccagcaga tcgtcaagcg ttgccagctt catgcgatcc agctcgcgct gaatattttc
    18061 ctgcgggatt tcattcagct tacggctacc acccaaagca tggttgagca gacgacggcc
    18121 caggcttaca gaatcatcac gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc
    18181 ttttgagcta acgacaaagt tcagccacgc ggcattcggg cgagcgcccg gtgcggtaat
    18241 gatttcaacg gtttgaccgc tggtaagcgg ctgcgacagc gggtaaggct ggcggtcaac
    18301 gcgtgcgccc acgcaggcat gaccgatatc ggtatgcact gcataagcga agtcgacggg
    18361 cgttgcaccg gcaggcagct cgacaattcg cccttccggt gtgaaaacgt aaatctcatc
    18421 cgggaagaga tcggatttaa cgctctcgat aaattcaaac gaactaccgg cgctctgttg
    18481 cagctccagc aggctttgca tccagcgctg ggcgcggatt tgcgcggtgg tgctggtttc
    18541 gccgtgctct ttataagccc agtgcgcggc aacacccatc tccgccatct gatccatatc
    18601 ttcggtacgg atctggacct caaccggcac accgtgcggg ccgatcatcg aggtgtgcaa
    18661 agactgatag ccgttcgctt ttggaatggc gatgtagtct ttcacgcgac ccggacgcgg
    18721 cttgtacagg ctatgcattt gcccgagcac gcgataacag gtgtcggaat cattgacgat
    18781 cacgcggaaa gcgtagatgt ccatgattga gtgaaaacgc tgctctttga gcaccatttt
    18841 gcagtaaatc gaataaagat gcttctcgcg accactgacg cggcagggaa ttcccgcttc
    18901 ctgcaaacgc ccttcgattt ccgagaggat tttttggatc atctctttac ggttgccgcg
    18961 cgcggctttc accacttctt tgattacgcg ataacggttg ggatacagcg cctcaaaacc
    19021 cagctcttcg agttcggttt taatgtggtg gatacctaaa cggtgcgcca gcgggctgta
    19081 aatttcgaga gtttcacggg cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt
    19141 gcgcatgttg tgggtacggt cggcaagttt gatgaggatg acgcggatat cctgcaccat
    19201 cgccataatc atcttgcgaa agttttcggc ctgcgcctct ttcttatcgc ggaacttgag
    19261 tttatcaagt ttcgacaccc cctctaccag ctcggcgacg cttttaccaa aaagctgttc
    19321 catatcctgg taggtggcgg gagtatcttc aatcacgtca tgcagcagcg ccgccatcag
    19381 cgtttcatag tcgagtttca tctcggccag aatgcaggca accgctaccg ggtgcgtgat
    19441 atagggttca ccgcttgaac gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc
    19501 ctgccgcaga cgcttgattt ggtcttccgg caggtaggtt tgaatcagtt gattcaggct
    19561 ttcaaacaga tacaagggcg acccgctttg tgattaacga cgaccttcag caatagcggt
    19621 aacggcttgt aattcagcgg cttccagctc ttgctgttcc tggcgttcgc gaacgtcgag
    19681 gatctggttg ttgatcagac cttcttcgat ttcgcgcagc gcgattacag tggttttatc
    19741 gttttcttcc ggtaccagcg gatcctttcc gcctacctgc atctgacgag cgcgacgcgc
    19801 ggcgaccagt accaggtcaa aacggttacc aattttctct acagcgtcct gaacagttac
    19861 gcgtgccata cttaaaaagc tccacaggtg aagaaatgac tgggcatgat actgaaatca
    19921 ggttcagtct gccaacaatt tgctgattaa agcgtcatga cgctgctttt ggcggctcat
    19981 gcgcagacgt tcggcgcgaa taatggtctt caaatcggtc aacgcggtat cgaagtcatc
    20041 attcacaatc agataatcat attcggcgta atggctcatt tctgcaacag cttgcgccat
    20101 acgctttgca atgacctctt cgctgtcctg accgcgaccg cgtagacggc ggtccagttc
    20161 aattttggac ggcggtaaaa taaagatact ccgcgcgtgc ggcatcttct ggcgaatttg
    20221 ctgcgcgccc tgccagtcga tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc
    20281 aatggcctca cgcgaagtgc catagtaatt accaaaaact tctgcgtgtt cgaggaacgc
    20341 atctctgcta atcatttctt taaattcatc atgattaaca aagaaataat gttcaccgtg
    20401 gacttcacca ggacgcggtt ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa
    20461 cggttgggtt tttaataaag cctgaatcag gctggattta cccgcgccac tgggggcaga
    20521 aacaatataa agcgtgcctt gagccatgag tatctttcgt atgtgattag cgaaataaag
    20581 cctacatacg agcttattat acacggcgcg gccatgtgac gtagcccttg ctcacacttt
    20641 ttgcgcaaag ttgttgcgtt ttgcgtagct gtttccgcat ttgcctgtga tttactgcaa
    20701 taaagacgat tctcaggaag cgtcctcgca gactccgttt ttgccactcg gctttgcctt
    20761 caccacgttg tatatctgcc agcctgacta cagcggaggt gaagatgaaa gtatggatgg
    20821 cgatattaat aagtatcttg tgctggcaat catctgtgtg ggcggtctgt ccggcctggt
    20881 cgccagccag agcacaggaa gaaatttccc gcctgcaaca gcaaataaaa cagtgggacg
    20941 atgactactg gaaggaagga aaaagtgagg tggaagacgg tgtttacgat cagttaagcg
    21001 cccgtcttac gcagtggcaa cgctgctttg tgagcgagcc ccgcgacgtg atgatgccgc
    21061 ctttaaatgg tgcggttatg catccggttg cccatacggg tgtacgcaaa atggcggata
    21121 aaaacgcatt aagtctgtgg atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg
    21181 atggcgttgc ggtaaccctg gtttatcggg acgggaaact gaacaaagca atcagtcgcg
    21241 gtaatggcct gaaaggcgag gactggacgc agaaagttag cttaatttcc gctgtgctgc
    21301 aaaccgttag cgggccttta gccaacagta cgcttcaggg ggaaatattt ctccagcgcg
    21361 aggggcatat ccaacaacaa atggggggaa taaatgcccg cgcaaaagtt gctggcctga
    21421 tgatgcgcca ggggaatagc gacacgctaa actcactggc tgtttttgtc tgggcatggc
    21481 cggatggacc gcagttaatg accgatcgtt taaaggagct ggctaccgca ggttttactc
    21541 taacgcagag gtatacccgt gcggttaaaa atgctgatga ggttgcgcgc gtacgcaatg
    21601 agtggtggaa agcgaaatta cccttcgtca ccgatggcgt ggttgtgcga ggggctaagg
    21661 aacccgaatc ccgtcattgg ctaccgggcc aggcggagtg gctggtggcc tggaaatatc
    21721 aacctgtagc tcaggttgcc gaagtgaagg cgattcagtt tgcggtgggt aagagcggta
    21781 aaatatcggt ggttgcgtca ctcgcacctg tcatgctgga tgataaaaaa gtccagcggg
    21841 tgaatattgg ttccgtcagg cgctggcagg agtgggatat tgcgcctggt gatcagatcc
    21901 tcgtcagcct tgccggtcag ggtattcctc gcattgatga tgtggtctgg cgcggtgcag
    21961 aacgtacaaa accgacacca ccagaaaacc gctttaaccc gttgacctgc tactttgctt
    22021 ctgatgtttg tcaggaacag ttcatttcac gcttagtctg gctggggtca aaacaggttc
    22081 tggggctgga tggcattggt gaggccggtt ggcgcgcgct gcatcagact catcgctttg
    22141 agcatatctt ttcctggctt ttattaacgc cagagcaatt acagaacacg ccggggatcg
    22201 cgaaaagtaa aagtgcgcag ttatggcatc agtttaatct ggctcgtaac cagcctttta
    22261 ctcgctgggt gatggcaatg ggaataccgc taacccgggc ggcgcttaat gccagtgatg
    22321 aacggtcctg gtcgcaactt ttatttagca cggagcagtt ctggcagcag ctgccgggga
    22381 ctggatctgg gcgcgccaga caggttattg aatggaagga aaatgcgcaa atcaagaagc
    22441 tgggcagttg gctggctgcc cagcagatca caggttttga accttagtga ccttcgtggc
    22501 tgtagtaaaa gaccggtaat cccagcttaa gacgtagcgc cagtaaacga gcaaaaaagc
    22561 cgaataccag ggtggagata atcaccacat catggttaga aacatagtgt tgcagcgcga
    22621 tgtacagcac ggcggaggca aatgagacac cggcatacaa ctctttttga aagaccagcg
    22681 gaatgcgttt acagaacata tcgcgcaaaa cgccgccaaa tacaccggtg gttaccgccg
    22741 cgacaacggc aataattggg ccgtgcccca tatccagtgc aacctgtgcg ccgatgatag
    22801 aaaagaccac cagtccgagc gcatcgagca ccagaaacac tttgcgcagg taaggcatta
    22861 caggagcaac gatagtggtg agtactgcgg cggtagcgac gataatcaca tattccgggt
    22921 gtttgaccca gccgagcgga tagtggccca gcagaatatc gcgcactgac cctccgccaa
    22981 ttgcggtggc ggttgcaata ataattacgc caaatgtatc catgcgccgt cgcccggccg
    23041 ccagcgcccc cgtcatggct tcggcagtaa tgccaaccaa atacaaaatg tgtaacagca
    23101 tggttctctc cctaaaaatc tgtctggcag ggtagcgatt tgtgcgcatt gtcacgattg
    23161 atatttttta aggtgagagg aaattgataa cttttagtta tgtgaaatcg ctattttctg
    23221 tagcgtagat gcattcttct gacttcttgt ctgagaaaat gcataagaaa aaataggtta
    23281 aaactgaggg gggaaaagaa aggtgcaatg tttagccatt gtgttcactg cacctggacc
    23341 ttggcttacg catcgggtgt ctcaatcaac aaaaagagtt tttcgtttct gctatttctt
    23401 ctctgtaatt tttacactgt tttccaactg cttgatgctg acctgcgggg tcggcaactc
    23461 ctcaggtatg gttccgccaa gttcctgaat ggtttgcctc accttgctac ccacgtcaaa
    23521 gtgtgtggta ttagcttgct gttttgaatt cacgctatcc cgcttgagtt tttcttctgt
    23581 ttgggtagct cgaaagagat tagccgccag ttctgttgaa cccatatgat ccaggatctt
    23641 ctgactcttt ttcagcccct tcagttggtg gatggctttc tgatctaatc caccatacag
    23701 cccctggtaa ccatgattct gaaatatggc gaaatcagta gctgtcgcta ccccagcttg
    23761 ctgtgctgcc tcaaccaatt gtttgttatg ttctttcaac tcatttctca gaaacagacg
    23821 cttttcgtct tcacgaagtt gcttaaatgc ttcatcgtca gcaagctcct gccgtcgggt
    23881 ctggatagta aaataagtct gccctgccgc aatgaccggt ttcgcggggt cgccgttttg
    23941 tactaccagg taacaggcat agcgggagag tacgatgtct tttaatgctc ttttggcatt
    24001 tgagccaatt ttgaccatat cgaggatctc ctcgaaatgg tcatcaattg tgtgaccact
    24061 gttctcacag gcctctttgg cgcgcgttaa tacaggtata aagtgacgat actctgaata
    24121 acccagtagt ttcccaagtt gtcttgctga ccattgttct gctccgtttg cattaatcag
    24181 ttttatctct tcaaaaggtt gatgatgttc gttcattgcc cactccatgt gatatcgatt
    24241 gcaaagaaag taaatctgta actgtattta tatacagttg tgagtatctc acgttctcag
    24301 tgtgctgggc aaaaaaacat cgcaaaatct ggagcaggca cgcaaaacag aattgaagtt
    24361 gcggggaaga aaaatgccgc tctgtttgag tgggcggcat ttgtgaaggc tgaaaagtca
    24421 gaattggtgt tacgagttac tcaatattct gaatctgctc gcgcatctgc tcaatcaaca
    24481 ctttcagctc gatggcggag tttgtcactt cggcattgat agatttcgac gcaagagtgt
    24541 tcgactcacg gttgaactcc tgcatcataa agtccagacg acgaccaacc gcttcttttt
    24601 tcttcagaat gttgtacgtc tctttaacat gcgcttcgag gcggtccagc tcttcggcaa
    24661 cgtcaattcg ttgtgccagc agaaccaact cctgctccag acggttgttt tccaactgaa
    24721 cctgagcatc ttccagcttc gcgaccagac gctcacgctg ccattgcaga atttccggca
    24781 tatgggcgcg gactttgacc acttcggcgg tgacgccttc cagacgctgc tcgatcaatg
    24841 ctttcagtgc ctgaccttcg gtttcgcgcg cgacgataaa gtcgtccagc gtaccatcca
    24901 acgccgcgag aatttcagcg gcaatcgcgt cgagatcctg ctcctgggct gccatcacgc
    24961 ccggccagcg tagaatatca accgggttga tttccccttc gtcactcttc atttttaccc
    25021 agttcgcggc agttaccagc tgtttagcca gtttttcatt gaggatcagc tcaccttgtg
    25081 cgctaacatc tggctcatag cgcagggtac attccacttt accgcgcgtc aggcgagaac
    25141 gaatacgctc gcgaacgaca ggttcaaggc tacggaactg ctccggcaga cgaaagtaag
    25201 tttccagata acgctggttt accgagcgca tttcccaggt tgcgctcccc cattcaccct
    25261 tgatttcacg ccgggcgtag gcggtcatac tgcggatcat agacgttcct gtttataaaa
    25321 ggagaggtgg aaggattata gccatcgatg ccttgtcagg ataggaataa ccgccggaag
    25381 tccgtataat gcgcagccac atttgtttca agccggagat ttcaatatgc gtccagcagg
    25441 ccgtagcaat aatcaggtgc gtcccgttac cctgactcgt aactatacaa aacatgcaga
    25501 aggctcggtg ctggtcgaat ttggcgatac caaagtattg tgtaccgcct ctattgaaga
    25561 aggcgtgccg cgcttcctga aaggtcaggg ccagggctgg atcaccgcag agtacggcat
    25621 gctgccacgt tctacccaca cccgtaacgc tcgtgaagcg gcgaaaggta agcagggtgg
    25681 acgcacaatg gaaatccagc gtctgatcgc ccgtgctctt cgcgcggcag tagatttgaa
    25741 agcgctgggt gagttcacca ttacgctgga ctgcgacgtg cttcaggctg atggtggcac
    25801 tcgtaccgcg tcgattacgg gtgcctgcgt ggcgctggca gatgcgctac agaagctggt
    25861 ggaa
//
LOCUS       DAJTPG010000053        25830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTPG010000053 DAJTPG010000000
VERSION     DAJTPG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..153)
                     /locus_tag="OO230_002275"
     CDS             complement(<1..153)
                     /locus_tag="OO230_002275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5692014.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTV"
     gene            complement(286..591)
                     /locus_tag="OO230_002276"
                     /pseudo
     CDS             complement(286..591)
                     /locus_tag="OO230_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            659..1274
                     /gene="tnpA"
                     /locus_tag="OO230_002277"
                     /pseudo
     CDS             659..1274
                     /gene="tnpA"
                     /locus_tag="OO230_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1602..2684
                     /locus_tag="OO230_002278"
     CDS             1602..2684
                     /locus_tag="OO230_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5692015.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3173..3971)
                     /locus_tag="OO230_002279"
                     /pseudo
     CDS             complement(3173..3971)
                     /locus_tag="OO230_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3981..4532)
                     /gene="ybcL"
                     /locus_tag="OO230_002280"
     CDS             complement(3981..4532)
                     /gene="ybcL"
                     /locus_tag="OO230_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5692016.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4701..5033)
                     /gene="emrE"
                     /locus_tag="OO230_002281"
     CDS             complement(4701..5033)
                     /gene="emrE"
                     /locus_tag="OO230_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5692017.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5378..5692)
                     /gene="fliE"
                     /locus_tag="OO230_002282"
     CDS             complement(5378..5692)
                     /gene="fliE"
                     /locus_tag="OO230_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5692018.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5906..7565
                     /gene="fliF"
                     /locus_tag="OO230_002283"
                     /pseudo
     CDS             5906..7565
                     /gene="fliF"
                     /locus_tag="OO230_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7558..8553
                     /gene="fliG"
                     /locus_tag="OO230_002284"
     CDS             7558..8553
                     /gene="fliG"
                     /locus_tag="OO230_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5692019.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8546..9232
                     /gene="fliH"
                     /locus_tag="OO230_002285"
     CDS             8546..9232
                     /gene="fliH"
                     /locus_tag="OO230_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5692020.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9232..10605
                     /gene="fliI"
                     /locus_tag="OO230_002286"
     CDS             9232..10605
                     /gene="fliI"
                     /locus_tag="OO230_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5692021.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10627..10968
                     /gene="fliJ"
                     /locus_tag="OO230_002287"
                     /pseudo
     CDS             10627..10968
                     /gene="fliJ"
                     /locus_tag="OO230_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10965..12092
                     /gene="fliK"
                     /locus_tag="OO230_002288"
     CDS             10965..12092
                     /gene="fliK"
                     /locus_tag="OO230_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5692022.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12197..12661
                     /gene="fliL"
                     /locus_tag="OO230_002289"
     CDS             12197..12661
                     /gene="fliL"
                     /locus_tag="OO230_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5692023.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12666..13670
                     /gene="fliM"
                     /locus_tag="OO230_002290"
     CDS             12666..13670
                     /gene="fliM"
                     /locus_tag="OO230_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5692024.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13667..14080
                     /gene="fliN"
                     /locus_tag="OO230_002291"
     CDS             13667..14080
                     /gene="fliN"
                     /locus_tag="OO230_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5692025.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14083..14448
                     /gene="fliO"
                     /locus_tag="OO230_002292"
     CDS             14083..14448
                     /gene="fliO"
                     /locus_tag="OO230_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5692026.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14448..15241
                     /gene="fliP"
                     /locus_tag="OO230_002293"
                     /pseudo
     CDS             14448..15241
                     /gene="fliP"
                     /locus_tag="OO230_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            15251..15520
                     /gene="fliQ"
                     /locus_tag="OO230_002294"
     CDS             15251..15520
                     /gene="fliQ"
                     /locus_tag="OO230_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5692027.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15529..16314
                     /gene="fliR"
                     /locus_tag="OO230_002295"
     CDS             15529..16314
                     /gene="fliR"
                     /locus_tag="OO230_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5692028.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16599..17222
                     /gene="rcsA"
                     /locus_tag="OO230_002296"
     CDS             16599..17222
                     /gene="rcsA"
                     /locus_tag="OO230_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5692029.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17266..17454)
                     /gene="dsrB"
                     /locus_tag="OO230_002297"
     CDS             complement(17266..17454)
                     /gene="dsrB"
                     /locus_tag="OO230_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5692030.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17617..17844
                     /gene="yodD"
                     /locus_tag="OO230_002298"
     CDS             17617..17844
                     /gene="yodD"
                     /locus_tag="OO230_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5692031.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18142..18957
                     /locus_tag="OO230_002299"
                     /pseudo
     CDS             18142..18957
                     /locus_tag="OO230_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18954..20648)
                     /gene="yedQ"
                     /locus_tag="OO230_002300"
                     /pseudo
     CDS             complement(18954..20648)
                     /gene="yedQ"
                     /locus_tag="OO230_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20819..21001)
                     /locus_tag="OO230_002301"
     CDS             complement(20819..21001)
                     /locus_tag="OO230_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5692032.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21080..21997)
                     /locus_tag="OO230_002302"
     CDS             complement(21080..21997)
                     /locus_tag="OO230_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5692033.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22170..23090
                     /gene="yedA"
                     /locus_tag="OO230_002303"
     CDS             22170..23090
                     /gene="yedA"
                     /locus_tag="OO230_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5692034.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23079..23549)
                     /gene="vsr"
                     /locus_tag="OO230_002304"
     CDS             complement(23079..23549)
                     /gene="vsr"
                     /locus_tag="OO230_002304"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5692035.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23530..24948)
                     /gene="dcm"
                     /locus_tag="OO230_002305"
     CDS             complement(23530..24948)
                     /gene="dcm"
                     /locus_tag="OO230_002305"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5692036.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25015..25555)
                     /locus_tag="OO230_002306"
                     /pseudo
     CDS             complement(25015..25555)
                     /locus_tag="OO230_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25595..>25830)
                     /locus_tag="OO230_002307"
     CDS             complement(25595..>25830)
                     /locus_tag="OO230_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692037.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6511 a         6630 c         6367 g         6322 t
ORIGIN      
        1 gactgtgcca tctgacagcg aggcaagttt agccacgcca gcatccagcc cgaccattga
       61 tgctgaaggg tgaaccggag ttgatacttc actttctgcc tgaatactga tgtaccactt
      121 accgcaggac tggctgacag tgacattttt cacaacaccc gtgacctgac ggctgttccg
      181 gtagcgcatc cagccaagtt ttggcagaaa aatacggctg ttttcctgag cgagcttaac
      241 gccctgcgga tagcggaatg catcattctg tcaccgcttt ttgaatcagg ggaacgccga
      301 gcgcttctgg aaaaagtttt tgcaggcctg ctcagggtct ttcagtgact gttgcaatgg
      361 ctgtgacgga gcatctttaa tccattgcgt ttcagtggca tttttccact caaccaatca
      421 ggaagccatt ttcgcgtaag ggatgtcttt gttaccggcc ttatgattct cattctgacg
      481 tgccagagta cgactgaaaa cgaaacgaca tgcgcctgcg aagcgcctca tttcacgcgc
      541 ctgttgacca ccgggtctta accggaattt gaatacctgt agtcgtttca cagggcaaat
      601 tatactttgg tctgtgaaaa aaactgatat ccgacgcgga aggcactgtg ttttcctgat
      661 gcatgctcac cgggtctttg ttaccaagta ccgacgccag atttttgacc atgacgcgac
      721 agaaaaacta cgcacttact tttcaaatgt atgtgcttat tttgaagctg aactggttga
      781 aatggatggc gaaccagatc acgtccatct gttaattaac tatcccccca aactggcgat
      841 atccagtctg gtaaacaaac agcctcaaag gcgtatcggg taggttactg cgacgggatc
      901 gaccagatat tgctgccagg tattaccgca aagatgtttt gtggcgtccc ggctattttg
      961 ccagtagctg cggaggtgcg ccaatatccg tcatccgcca atacattgaa caacagcaaa
     1021 cacctggtca gatgaaaaaa cgagccttat atccccgccc tgaaggacgg gcttttacgg
     1081 tgcactggat aagcctggag attatctaca cctgcatgga catcattgta cgatgccccg
     1141 tacatgctcc atcacatcct caacatcaaa gtattagagc cttgcaaacc attatgcgtc
     1201 tcagtttgtc tcatctatct tacgcaaaaa aaatagaact aatatcaagc ggcgctatat
     1261 tgcgtattaa ataaaaccac ctcaattaca ttaataaatc taaataaata tcatgcaaat
     1321 aatttccatg gttattttta ggctatttaa attaaaaaaa taaattatga gtcaatcata
     1381 aataacaacc tttaatataa tttgactacc aaatttatat tcgttcacaa aatagtcatg
     1441 cattgtgtgc aatagaaaca gttcagataa aaatagggat ctacttcaca atttaaatga
     1501 gaaactaaaa cttacatctt gaaataatct cattgattgg atgaatattt gtagtgcagt
     1561 gacatcattt ttaaataata gttcaaataa aagggctcac gatgaaaaaa ttaacagtgg
     1621 caatttctgc tgtagctgca tcagtactga tggcgatgtc tgctcaggca gcagagattt
     1681 acaataaaga cagtaacaag ctggatctgt acggaaaagt taatgctaag cactacttct
     1741 cttctaacga tgcagatgat ggcgacacaa cttatgttcg tctgggcttc aaaggcgaaa
     1801 ctcaaatcaa cgatcaactg actggtttcg gtcagtggga atatgaattc aaaggcaacc
     1861 gtgctgaatc tcaaggttct tccaaagata aaacccgtct tgccttcgct ggcctgaaat
     1921 tcggtgacta cggctccatc gattatggcc gtaactacgg tgtagcatac gatatcggtg
     1981 catggaccga cgttctgcca gaattcggtg gcgatacctg gacccaaaca gatgtgttca
     2041 tgactggtcg caccacaggt gttgcaacct atcgtaacaa cgacttcttt ggtctggttg
     2101 atggtctgaa ctttgctgct cagtatcagg gtaaaaatga ccgcactgac gtaactgaag
     2161 ctaacggtga tggtttcggt ttctccacta cttatgagta tgaaggattc ggtgtaggtg
     2221 caacctatgc taaatctgac cgcactaatg atcaggttat ctacggtaac aacagcctga
     2281 atgcatctgg tcaaaatgct gaagtatggg cagctggtct gaaatatgat gcgaacaaca
     2341 tctatctggc taccacctat tctgaaaccc agaacatgac tgtttttggt aataaccata
     2401 ttgccaacaa agcacaaaac ttcgaagtag ttgcacaata tcagttcgac ttcggtctgc
     2461 gtccgtccgt tgcttacctg caatctaaag gaaaagactt gggtgcgtgg ggtgatcagg
     2521 acctgattga atatattgat gtaggtgcaa cctattactt caacaaaaat atgtccactt
     2581 ttgttgatta caaaatcaac ctgattgata agagcgattt cacgaaagca tctggcgttg
     2641 ctaccgatga tatcgttgct gtaggattgg tttaccagtt ctaatctgat tactcaaaga
     2701 tatgttgtgg gaggctttgc ctccccaaca tataagtggc gctctcaagc cactttcctt
     2761 agaagcacta tcttgcttct gactatataa attacccttt attttctggg acgtttcaac
     2821 gccccatttt taataatact cagtgaacaa ttaacgtatc aattagattt aataacaacg
     2881 atatccgtac ctaacctgat atctaatgtc ataagcatcc ctttaattat cccctcaacc
     2941 tcttgtaaat ttcccactct tgagctccac atagcccaaa cgccggatgc aaaaaaaatc
     3001 atacgtgcat ctctgaaatt gtgatggagg aagtgagtat caattatatg tgcttataaa
     3061 cgttcccgat tggcacagaa gtcaccggat tgttcaggct ccaataggtt gagtatgtcg
     3121 tatttatttt ccaaaataaa aatcgatgct gttgctcctt aataaaatac ttttactctc
     3181 tggacgcttt tcttagctct cttggtgtta tattaaaacg attatggaac ctttcagtaa
     3241 aacgagaagg acacttgtaa ccattttccc tggcaatctc gctgatgggt tttactgtcg
     3301 tttgtatagc aggacaacgc attatttaac ctcacgtcgt ccaatatact ttgaaaactt
     3361 attccctcgc ttgccagacg gcgatgcaat gtggaaacag aaatgtagag atatctcgct
     3421 accttgtttg ctgtccattt tgttctaggt tcggatagca ttaggttata acaacgactt
     3481 attaatgatt gtttactatc tgataaaagt aaacaattaa catgatgtgc tcctaatgaa
     3541 agtagaacgc ccattgctaa gtgttcctga atttgcgttg agaagcctcg ggaaatagat
     3601 gtttttagtt gctcccaaca atatattaac tcggggttct gaggtaagca gaaacctgtc
     3661 gtgttacgaa tttgatcagt tatcgcataa aatttttgaa acttttcaat tagatcaatg
     3721 ggataataaa gcatttctgc aagataaaga cctgcttctg gataattcac aatataaaat
     3781 tcatatccac agggaaataa tattatctga ttattatcaa cagttagact atgcgtctcc
     3841 caattgataa ctttttttcc ctgacggata cgacataaag ctggcataag aggcttaacc
     3901 ctgtgaatct catgatgttt atgcatccgt atttcttcaa tctttaggtt agacttatct
     3961 cttgccagca tactcttacc ctattttatc tcataaactg gtgttatctc agcggttgca
     4021 attttattgg cattaagcat ataaccaact aacgctccgc tggaattaga atccacagga
     4081 atcttatcag tttttagagc ccatacttta aactggtaat gatggggttt atctcctttg
     4141 ggaggacatg ctccaccaaa tcctgcataa ccaaaatcat ttcggccttg aacagcacca
     4201 gtcggaagtt ttgtaccatc acgtcttcct gcatcagtgg gcaaatatgt tacagttgct
     4261 ggaatattag caacagtcca gtgccaccat ccactgcctg taggagcatc tggatcatac
     4321 acagttacgg caaagctttt ggtgccttca ggagcaccag accaggttaa tgagggcgat
     4381 gtattaccac cttcacaccc aaatccagaa aatacatgag acgttgtaag ttgctctcct
     4441 gtttttattt cattactagt gacctgaaat gctgcggcct gcgcagaaaa tgttatgaat
     4501 gccaatacac ttgaaacgat aagttttttc ataaaaacct cattgttgtg acctatcgtt
     4561 attttatttg atgtgccttg atctcattat gcataaaggc gcaatgatcg cgcaaatgca
     4621 atcataatta taaaggtgcc ccgaacatta gccataatat gcacaaatca gattcctgtt
     4681 atctcaagga gaattatttt ttaatgcggt gcacttcgtg acaacaagtt aatgactaac
     4741 acaccggcac aaatcaacat catgcctatg atagctggca ggtccagtcg ttgaccaaaa
     4801 aatccccagg aaagcaaact aatcagaacg ataccgactc ctgaccagat agcataagca
     4861 atccctgtag gtatataagc cagcgtctga gctaataacc agaatgatgc acaatagcaa
     4921 ataattgtgc caacagaggg ccataaccgt gtaaacccct ctgaaaactt cattaaggtt
     4981 gtaccaatga cctctgcaag tattgcgcca ccaagataaa tataagggtt catagcatat
     5041 tctttcctgt tcatactgga gagaattgta ctacagtttg aacacaactc accgttttca
     5101 tcctggtgta tgtgtatcca aggggctctg gcgtggcaag agataaaggc aggaacaata
     5161 atgatcagaa ggaaccccag gctaacgaat gagttcactc ttcctgggag caaagcgaag
     5221 acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga aaacaaccag gatcaggtat
     5281 ccattttaaa ccagaaattg agtgagtttg ccccatccat gccccacgct gtgggatcgg
     5341 atataattaa acaagcatag tcaacaggtt acacaaccta cacctgcatg gacatcactt
     5401 cctgatacgc cgccaccagc ttattacgca cctgaatccc catttgcata gaaactgagg
     5461 ctttttgcat atcggtcatc acatcgttta gcgccacgcc gggttcaccg agggtgaatt
     5521 tttctgcctg cgtgcgggcg actgtttgtg tatcgcttat gcgatcgagc gcggcgtgca
     5581 gctgcccggc aaaactaatg gtcggttgcg gcagtgattc ctgcgcacgc gcactcatcg
     5641 ccgtagcctg taactggctg ataacccctt caatcccctg tatcgctgac attttcgtct
     5701 cccggataat ttctggtaac aaagcctacc agtaagtcaa taagacaaag gcgctaaata
     5761 gcaacaaaaa aactggttta ttggcggata gaaaaaacga aagcacaaat aatgggagcg
     5821 tcaatttttc gagtgtgctg acccgagagt gagtcttgtt ccactttgcc aataacgccg
     5881 tccataatca gccacgaagt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga
     5941 gtggcttaat cgcctgcgtg cgaatccgaa aattccattg attgttaccg gttccgcggc
     6001 agtggcggtc atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt
     6061 cagcaatctt tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat
     6121 tccttaccgc ttcagcgaag ccagcggcgc tattgaggtt ccggcagata aagttcacga
     6181 actgcgcctg gcgcaacaag gtttgcccaa aggcggcgcg gtcggtttcg aactgctcga
     6241 tcaggaaaag tttggtatca gccagttcag cgaacaggtg aattatcagc gggcgctgga
     6301 aggcgagctt tctcgtacca tcgaaactat cggcccggta aaaggggcgc gcgtacatct
     6361 ggcaatgccg aaaccgtctt tattcgtccg tgaacaaaaa tccccttctg catcggtgac
     6421 ggtaaatctg ttacccggcc gcgcactcga tgaagggcaa attagcgcta ttgtacatct
     6481 ggtttccagc gcggttgctg gtctgccgcc tggaaacgtc acgctggtgg atcagggcgg
     6541 gcatctgtta acccagtcca ataccagcgg gcgcgatctt aatgacgctc agttgaaata
     6601 tgccagcgat gtcgaaggcc gtattcagca gcgtattgaa gcgatcctgt cacctattgt
     6661 tggtaacggt aatattcacg cctaggtcac ggcgcaactg gacttcgcca gtaaagaaca
     6721 aacggaagaa cagtatcgcc ctaacggtga tgaatctcat gcggcgcttc gttcacgcca
     6781 gcttaatgag agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg gggcgttgtc
     6841 gaatcaaccg gcacctgcga ataacgcgcc aatcagcacg cctccgacaa atcaaaataa
     6901 ccgccagcag caggcgagca ccaccagcaa tagtgggccg cgtagcacac agcggaatga
     6961 aaccagtaac tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg tgggcgatgt
     7021 gcaacgtctg tcagtcgcgg tagtggtgaa ttacaaaacc ttgccagacg gtaaaccatt
     7081 gcctctcagc aacgaacaga tgaagcaaat tgaagctctg acccgcgagg cgatgggctt
     7141 ttctgaaaaa cgcggtgatt cgctcaatgt tgttaactcg ccgttcaata gcagtgacga
     7201 aagcggcgga gcactgccgt tttggcaaca gcaagtgttt atcgatcagt tgctggctgc
     7261 cggtcgctgg ttgctggttg ctggtactgc tggtggcgtg gttgctgtgg cggaaagcgg
     7321 tacgcccgca gctaacacgt cgcgccgagg cggtgaagac tgtgcagcaa caggcgcagg
     7381 cccgcgagga agtggaagat gcggtggaag tccgtctgag caaagacgaa caactccaac
     7441 aacggcgcgc taaccaacga ctgggggcag aagtcatgag ccagcgtatc cgtgaaatgt
     7501 ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat aacgatcatg
     7561 agtaacctga caggcaccga taaaagcgtc atcctgctga tgaccattgg cgaagaccgg
     7621 gcggcagagg tgttcaagca cctctcccag cgcgaagtgc aaaccctgag cgctgcaatg
     7681 gcgaacgtca cgcagatctc caacaagcag ctaaccgatg tgctggcgga gtttgagcaa
     7741 gaagctgaac agtttgctgc actgaatatc aacgccaacg attatctgcg ttcggtattg
     7801 gtcaaagctc tgggtgaaga acgtgccgcc agcctgctgg aagatattct cgaaacccgc
     7861 gataccgcca gcggtattga aacgctcaac tttatggagc cgcagagcgc cgccgatctg
     7921 attcgcgatg agcatccgca aattatcgcc accattctgg tgcatctgag gcgcgcccag
     7981 gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat gttgcgtatc
     8041 gccacctttg gcggcgtgca gccagccgcg ctggccgaac tgaccgaagt actgaatggc
     8101 ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac ggcagccgaa
     8161 attatcaacc tgatgaaaac gcagcaggaa gaagccgtta ttaccgccgt gcgtgaattc
     8221 gacggcgagc tggcgcagaa aatcatcgac gagatgttcc tgttcgagaa tctggtggat
     8281 gtcgatgatc gcagcattca gcgtctgttg caggaagtgg attccgaatc gctgctgatc
     8341 gcgctgaaag gggccgagca gccactgcgc gagaaattcc tgcgcaatat gtcgcagcgt
     8401 gccgccgata ttctgcgcga cgatctcgcc aagcgtggtc cggtgcgtct gtcgcaggtc
     8461 gaaaacgaac agaaagcgat tctgctgatt gtgcgccgtc ttgccgaaac tggcgagatg
     8521 gtgattggca gcggcgagga tacctatgtc tgataatctg ccgtggaaaa cctggacgcc
     8581 ggacgatctc gcgccaccac cggcagagtt tgtgccaatg gtcgagtcgg aagaaaccat
     8641 tattgaagag gccgaaccca gccttgagca gcaactggcg caactgcaaa tgcaggccca
     8701 cgagcagggt tatcaggcgg ggattgccga aggtcgccag caaggtcacg aacagggcta
     8761 tcaggaagga ctggcccagg ggctggaaca aggtctggca gaggcgaagg ctcaacaagc
     8821 gccaattcat gcccggatgc agcaactggt cagcgaattt caaactaccc ttgatgcgct
     8881 tgatagtgtg atagcttcgc gcctgatgca gatggcgctg gaggcagcac gtcaggtgat
     8941 cggtcagaca ccaacggtgg ataactcggc actgatcaaa cagatccaac agttgttgca
     9001 gcaagaaccg ttattcagcg ggaaaccaca gctgcgcgta cacccggatg atctgcaacg
     9061 tgtggatgat atgctcggcg caactttaag tttgcatggc tggcgcttgc ggggcgatcc
     9121 caccctccac cctggcggat gtaaagtctc cgccgatgaa ggcgatctcg acgccagtgt
     9181 cgccactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt aatgaccacg
     9241 cgcctgactc gctggctaac cacgctggat aactttgaag ccaaaatggc gcagttgcct
     9301 gcggtacgtc gctacgggcg attaacccgc gctaccgggc tggtgctgga agccaccgga
     9361 ttacaattgc cgctcggcgc aacctgtgtc attgagcgcc agaacggcag cgaaacgcac
     9421 gaagtagaaa gcgaagtcgt tggctttaac ggtcaacggc tgtttttaat gccgctggag
     9481 gaagtggaag gtgtcctgcc cggcgcgcgt gtatatgcca aaaacatttc ggcagaaggg
     9541 ctgcaaagcg gcaagcagtt gccgctcggt ccggcgttat taggtcgtgt tctggacggt
     9601 agcggtaaac cgctcgatgg cctgcccttc cccgatacga cggaaaccgg tgcgctgatt
     9661 accccgccat ttaacccgtt gcaacgtaca ccgattgaac atgtgctgga caccggcgtg
     9721 cgcccaatca atgccctgct taccgtcgga cgcgggcagc gtatggggct gtttgccggg
     9781 tcgggcgttg gtaaaagtgt gctgctgggg atgatggcac gctacacccg cgccgatgtc
     9841 attgtcgtgg gtttgattgg tgaacgtggg cgcgaagtaa aagattttat tgagaatatc
     9901 ctcggtgccg aaggacgtgc acgctcagtg gtgattgccg ctccggcgga tgtttctccg
     9961 ctcctgcgaa tgcaaggtgc cgcctatgcc acgcgcattg ccgaagattt tcgcgatcgt
    10021 ggtcagcatg tgttgctgat tatggactcc ctcacccgct acgcgatggc ccagcgtgaa
    10081 attgcgctgg cgattggcga accacctgcc actaaaggtt atccaccgtc ggtgtttgcc
    10141 aaattaccgg cactggtcga gcgtgccggt aatggcatta gcggcggcgg ctcgattacc
    10201 gcgttttata ccgtgctcac cgaaggcgat gaccagcagg accccattgc tgactccgcg
    10261 cgggcaatcc tcgacggtca cattgtgctg tctcgccgac tggcggaagc cgggcactat
    10321 ccggctatcg atattgaagc gtcgatcagt cgcgcaatga cggcgttgat cagtgagcaa
    10381 cattacgcgc gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg taaccgcgat
    10441 ctggttagcg tcggcgcgta tgccaaaggc agcgatccga tgctcgataa agccatcgcc
    10501 ctgtggccgc agctggaggg ctatttgcaa caaggcattt ttgaacgcgc ggactgggaa
    10561 gcgtctctcc aggggctgga gcgtattttc ccgacagtgt cataacccag gagataacgg
    10621 cagatggcgg aagaacagct caaaatgttg atcgattatc agaatgaata tcgcaataac
    10681 ctcaatagcg atatgagtgc cgggatgacc agcaaccgct ggatcaacta tcagcagttt
    10741 atccagacgc tggaaaaagc cattactcag catcgccagc aacttaatca gtggacgcag
    10801 aaagttgaca ttgccctgaa cagctggcga gaaaagaaac aacgtttgca ggcctggcag
    10861 acactgcagg aacggcaatc cacggcggca ctgcttgcag aaaaccgcct cgatcagaaa
    10921 aagatggatg agttcgccca gcgcgccgcc atgaggaaac ctgaatgatt cgcttagcgc
    10981 ccttgattac cgccgacgtt gacaccacca cattgcctgg cggcaaagcc agcgatgctg
    11041 cacaagattt tctcacgttg ttgagcgaag cattagcagg cgagacaaca actgacaaag
    11101 cggcccccca gttgctggta gcaacagata agcccacgac aaaaggcgag ccgctggtca
    11161 gcgatattct tgccgatgcg caacaagcgg atttactgat ccctgtggat gaaacacttc
    11221 ctgtcatcaa cgacgaacaa tccacatcaa caccgttaac caccgctcag acgatgacgt
    11281 tggctgcggt ggctgacaaa aatacgacaa aagacgaaaa agcggatgat cttaatgaag
    11341 acgtcaccgc aagtctgagc gccctttttg cgatgttgcc gggttttgac aatacgccca
    11401 aagtgactga tgcaccgtca accgtgttac cggcagagaa accaacgctc ttcacaaaac
    11461 tgacttctgc gcaactcact acagcacagc ctgatgacgc ccccggcaca ccagctcagc
    11521 cattaacacc gctggtagca gaagcccaga gtaaagcgga agtcatcagc acgccttcgc
    11581 cggtgaccgc tgacgccagc ccgctaatca ctccacacca gacacagcca ctgcccaccg
    11641 tcgccgcgcc tgttttgagt gcaccgctgg gttctcacga atggcaacaa tcattgagcc
    11701 agcatatttc gctgttcacc cgccaggggc aacaaagtgc agagttgcgt ctgcacccac
    11761 aggatttagg tgaagtgcaa atttccctca aagtggatga taaccaggcg cagatccaga
    11821 tgatttcacc gcatcagcac gtacgcgccg ccctggaagc agcgctgccg gtactgcgta
    11881 cgcagctggc cgaaagtggc attcagttag ggcaaagcaa cattagtggc gaaagcttta
    11941 gtggtcagca gcaggccgct tcccagcaac agcaaagcca acgcacagca aaccatgaac
    12001 ctctggcggg ggaagacgac gatacgcttc cggttcccgt ctctttacaa gggcgtgtaa
    12061 caggtaacag cggcgttgat attttcgcct aacgtcagag gtagcaccgt aatccgcgtc
    12121 ttttccccgc tttgttgcgc tcaagacgca ggataattag ccgataagca gtagcgacac
    12181 aggaagaccg caacacatga ctgattacgc gataagcaag aaaagcaagc gatcgctttg
    12241 gatcccgatt ctggtattca ttaccctcgc ggcctgtgcc agcgcaggtt acagctactg
    12301 gcattcgcat caggttgccg ctgacgacaa agcgcagcaa cgcgtcgtgc cctccccggt
    12361 cttctatgcg ctggatacct ttacggtcaa tttgggcgat gcggatcgcg tgctttatat
    12421 cggcataacc ctgcgactga aagatgaagc aacccgctcg cggctgagtg agtatttgcc
    12481 ggaagtccgt agtcgcttgc tgttactgtt ttcacgtcag gatgctgccg tactggcgac
    12541 agaagaaggc aagaaaaacc tgattgccga gattaaaacc acactttcca ccccgcttgt
    12601 tgccgggcaa ccgaaacagg atgtcaccga cgtgctgtat accgctttta ttctgcgata
    12661 acgacatggg cgatagtatt ctttctcaag ctgaaattga tgcgctgttg aatggtgaca
    12721 gcgaagtcaa agacgaaccg acagccagta ttagcggcga aagtgacatt cgtccgtacg
    12781 atccgaatac ccaacgacgg gttgtgcgcg aacgtttgca ggcgctggaa atcattaatg
    12841 agcgctttgc ccgccatttt cgtatggggc tgttcaacct gctgcgccgt agcccggaca
    12901 ttaccgtcgg ggccatccgc attcagccgt accatgaatt tgcccgcaac ctgccggtgc
    12961 cgaccaacct gaacctaatc cacctgaaac cgctacgcgg cacagggctg gtagtgttct
    13021 cgccgagtct ggtgtttatc gccgtggata acctgtttgg cggcgatgga cgcttcccga
    13081 ccaaagtgga aggtcgcgag tttacccata ctgaacagcg cgtcatcaac cgtatgttga
    13141 agctggcgct ggaaggctat agcgacgcct ggaaagcgat taatccgctg gaagttgagt
    13201 acgtgcgttc ggaaatgcag gtggaattta ccaatatcac cacctcgccg aacgacattg
    13261 tggttaacac gccgttccat gtggagattg gcaacctgac cggcgaattt aatatctgcc
    13321 tgccattcag catgatcgag ccgctacggg aattgttggt taacccgccg ctggaaaact
    13381 cgcgtaatga agatcagaac tggcgcgata acctggtgcg ccaggtgcag cattcacagc
    13441 tggaactggt ggccaacttt gccgatatct cgctacgcct gtcgcagatt ttaaaactga
    13501 aacccggcga cgtcctgccg atagaaaaac ccgatcgcat catcgcccat gttgacggcg
    13561 tcccggttct gaccagtcag tacggtactc tcaacggtca gtatgcgtta cggatagaac
    13621 atttgattaa cccgatttta aattctctga acgaggaaca gcccaaatga gtgacatgaa
    13681 taatccggcc gatgacaaca acggcgcaat ggacgatctg tgggctgaag cgttgagcga
    13741 gcaaaaatca accagcgaaa aaagcgctgc cgacgcggtg ttccagcaat ttggcggtgg
    13801 tgatgtcagc ggaacgttgc aggatatcga cctgattatg gatattccgg tcaagctgac
    13861 cgtcgagctg ggccgtacgc ggatgaccat caaagagctg ttgcgtctga cgcaagggtc
    13921 agtcgtggcg ctggacggtc tggcgggcga accgctggat attctgatca acggttattt
    13981 aatcgcccag ggcgaagtgg tggttgttgc cgataaatat ggcgtgcgga tcaccgatat
    14041 cattaccccg tccgagcgga tgcgccgcct gagccgttag tgatgaataa ccatgctact
    14101 gtgcaatctt ccgcgccggt ttctgctgag ccactgctgc aggtgagcgg cgcactcatc
    14161 gccattattg ccctgatcct cgctgctgcc tggctggtaa aacggctggg atttgcccct
    14221 aaacgcactg gcgttaacgg tctgaaaatt agcgccagtg cttcgctggg cgcgcgtgaa
    14281 cgggtcgtgg tggtcgatgt ggaagatgca cggctggtgc tcggcgttac cgcagggcaa
    14341 atcaatctgc tgcataaact tcccccttca gcaccaacgg aagagatacc gcagaccgat
    14401 tttcagtcgg tcatgaaaaa tttgcttaag cgtggcggga gatcctgatg cgtcgtttgt
    14461 tttctgtcgc accggtcctt ctctggctga ttacgcccct cgccttcgcg caactgccgg
    14521 gtatcaccag ccagccgcta cctggcgggt atcaccagcc agccgctacc tggcgggtat
    14581 caccagccag ccgctacctg gcggtggaca aagctggtcg ctcccggtgc agacgctggt
    14641 gttcatcacc tcgttgacgt tcattccagc gattttactg atgatgacca gtttcacccg
    14701 catcattatt gtttttggtt tattgcgtaa cgcgctggga acgccctccg cgccacctaa
    14761 ccaggtattg ctggggctgg cactgttttt gacctttttt attatgtcgc cagtgatcga
    14821 caaaatttat gtcgatgcgt accagccatt cagcgaagag aaaatctcaa tgcaggaggc
    14881 gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg cgtcagaccc gcgaggcaga
    14941 tttagggttg tttgccagac tggcgaatac cggcccgttg caggggcctg aagctgtgcc
    15001 gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg aaaaccgcat ttcagatagg
    15061 cttcacgatt ttcatccctt ttttgattat cgacctggtg attgccagcg tgttgatggc
    15121 attggggatg atgatggttc cccccgccac cattgctctg ccctttaaac tgatgctgtt
    15181 tgtactggtg gatggctggc aattgctggt cggttcgctg gcgcagagct tttacagcta
    15241 gagaggcaaa atgacacctg aatcggtcat gatgatggga actgaagcga tgaaagtcgc
    15301 gctggcactg gcagccccgc tgttgctggt agcgctggtc acgggcctta tcatcagtat
    15361 tttgcaggca gccacgcaga ttaacgaaat gacgctgtcg tttattccga aaatcatcgc
    15421 cgtatttatc gccatcatta ttgccggacc gtggatgctc aatctgttgc tggattacgt
    15481 ccgcaccttg ttcactaacc tgccgtatat catcgggtag cccgtacaat gatgcaggag
    15541 acaagcgatc aatggctttc ctggttaagc ctgtacttct ggccgttact gcgcgtgctg
    15601 gcgctcattt ctaccgcgcc aattctgagc gaacgcagcg tgccgaaacg ggtaaaactg
    15661 ggtctggcaa tgatgatcac gttcgccatt gccccatcat tacctgccaa cgatgttcct
    15721 gttttttcgt tctttgctct gtggctggcc gtacagcaga tcctgatcgg cattgcgctt
    15781 ggttttacca tgcaatttgc ctttgccgct gtgcgaaccg ctggcgaaat tatcggtctg
    15841 caaatggggc tgtcatttgc gacatttgtc gatcctggca gccatctcaa tatgcccgtt
    15901 ttagcgcgca tcatggatat gctggcatta ctgctgttcc tgacatttaa cggtcattta
    15961 tggttgattt cactgctggt cgataccttt cacaccctgc cgattggtag cgaaccgttg
    16021 aacagcaatg cgtttctggc acccaccaaa gcagggagtt tgattttcct taacgggctg
    16081 atgctggcgt taccgctcat tactctgttg ctgaccctga atctggcatt aggtttactg
    16141 aatcgtatgg ccccgcaatt atccattttt gttattggat ttccattaac tctgactgtc
    16201 ggcatctctt taatggcggc attaatgccg ttaattgcgc ctttttgcga acatttattc
    16261 agtgaaattt ttaatttgct ggctgatatt attagtgaat taccattaat ataattccgt
    16321 aacgtttatc atgctaccct aaggattatc cgaaaaataa tatctgcgaa catcttccag
    16381 gatactcctg tagcgaaata tttgttttaa gctcactcac atatcgcaac atttacttta
    16441 agacaattcc aggcaaatta tacaacactt tacgggatag taagtccgcc tgaaaaattg
    16501 cgagagtggc gcattaggtg acccatgttg ttccgtttag tcatgatgaa atattcaggt
    16561 aaggggaatt atcgttacgc attgagtgag ggtatgccat gtcaacgatt attatggatt
    16621 tatgtagtta cacccgacta ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa
    16681 gagaaatcaa cgacattgaa accgttgatg accttgccat agcttgtgat tcacagcgtc
    16741 cttcagtggt gtttattaat gaggactgtt tcatccacga tgcttctaac agtcagcgca
    16801 tcaagcacat cattaatcaa catcccaata cgttatttat cgtttttatg gcaattgcca
    16861 atgttcattt tgatgaatat ctattggtca gaaaaaattt attgatcagt tctaaatcga
    16921 ttaaaccgga atctctcgac gatatccttg gcgatattct gaaaaaagag acaacgataa
    16981 cctcgttttt aaatatgccg acgttatcat tgagccgaac cgaatcgagt atgttgcgaa
    17041 tgtggatggc aggtcaggga accattcaaa tctccgacca aatgaatatc aaagccaaga
    17101 ccgtttcatc gcataaaggt aatattaaac gtaagatcaa aacgcataat aaacaggtta
    17161 tctaccatgt cgtccgactg acggataatg tgactaatgg tatttttgtc aacatgcgct
    17221 aacacattct gactggtggt ttcccaccag tcaggctgaa taagattact ctgctttctc
    17281 aacaaagata ccgtcctgat gccctgcttc attaaagaac cagatgccga gcgggtagtc
    17341 ttccagcgca accaggtaca ttgtgccttc actaaactcc tcaactgcca gtaccactcc
    17401 aggacgacgc ggaccgccat ccgttttgac tgttacccga tcattcacct tcatcgtttt
    17461 cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt tctggcagcg ccaagcgcgc
    17521 gcgagtgctg attttctcga cggtctatac ttaagagatg ccaggcggac ttaacgactg
    17581 gcggcaacaa cagagtaacg gttgcgagga aagatgatga aaaccgcaaa agtgtacagc
    17641 gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa
    17701 attagcgaaa gcgaagttca tcgcagccag aacgattctg aacacgttag cgtcgatgga
    17761 cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat tcatgacttc
    17821 agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct gagggtgtcg
    17881 ggatgaaact tgcttaagca agaagcactt aaaaaattcg ttacaccagg aaatctgatg
    17941 tgttcatcac cttatctgca atttttttcg ctgacaagaa aatattcgcg ctatgaatga
    18001 ttaacgctac ttatgaatag aaatgtgacg cgcgtcaccc cttggtggct ctggtgtcaa
    18061 cgggatcgct cccgaaaaaa tcatctttga ttatttgtgc tgttttgaga atcatcctgg
    18121 taaggggagt actgcgcgac catgttttca attcaacaac cactactggt ttttagcgat
    18181 cttgatggca ccctgctgga cagtcatagt tatgactggc aaccggcagc cccctggctc
    18241 agccgtttac acgaagccaa tattcccgtc attctctgta gcagtaaaac tttagcggaa
    18301 atgctgtact tgcaaaaaat gttggggcta caaggcttac cgctgattgc agagaatggc
    18361 gcagtgatcc agcttgctga acaatggcag gatatagacg gttttccacg catcatctca
    18421 ggtattagcc atggcgaaat ctgccaggtt ttaaatacgc tacgcgagaa agaacatttt
    18481 aaattcacga cttttgatga tgtcgacgat gcaaccattg ccgaatggac gggattaagc
    18541 cgtagctagg cggcgctgac gcagctgcat gaggcgtcgg taacgctaat ctggcgcgac
    18601 agtgacgagc gtatggcaca atttatcgct cgtctgaacg aactgggctt gcagtttatg
    18661 cagggtgcgc gcttctggca cgtactggat gcctctgccg gaaaagatca ggctgccaac
    18721 tggattatcg cgacctatca acaattgtca ggcagacgcc caaccacact tggcctgggc
    18781 gatgggccaa acgatgcgcc tttactggag gtaatggatt acgcggtgat tgtgaaaggg
    18841 ctaaaccgtg aaggggtaca tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag
    18901 cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg
    18961 ttatcgctcg cgaatacccg attgcgcccg gcctgttttg cgaggtaaag ccgacggtcg
    19021 gccaatgact ggagttgttc aaaatcataa tcaccggttt cctcgctgct acttaccccc
    19081 agcgaggcac tgatgcgtat cgttgtactc ttggcgatca gcatctcttt ttcatttaac
    19141 ttcaggcgaa tacgttctgc gacctccgca gcctgcgtca gattcgcgcc tggcagaatc
    19201 acacaaaact cctcaccacc gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta
    19261 attaatccgg cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta
    19321 atcgctttaa aatggtcaag atcgacctga atgacagaaa aaggatgttg gtgcgtctga
    19381 cacaatttag cgagcggacg ggctttttcg aacagtgcgc cacggttata taaacgcgtt
    19441 aaggtgtcgt gccacgcctg ccactgcaac gagctttgca gaacatacat gttgctgacc
    19501 atccggcgaa tcacatacca ggagaggagt aacatggtgg taaagagcgc ccacagcagg
    19561 gttaatgcaa tgctgatact gccgaaatcg ccgcgcacgc cttcgcttag cgtatggacg
    19621 cgcgccagca caccgtcgaa atggtccaga cgttcccagc taacatagcg actgtccata
    19681 cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag ttcacgagga
    19741 tcaaaaatat tccctgttgg atggtcagga ttggaagagg tcaaaaatct cagcttactg
    19801 tcatagagct gatactcacc atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc
    19861 atagtacgca cgggaatact catccccagc acgccatacc agtagttatt actgtctacc
    19921 ggaacactga cggtaacctg cggttcagta ttgctggcgt gttccggttg cgaagtgaac
    19981 cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc cgataaacca gggttgggtg
    20041 acatagccgt aataacgcgt tggtacattg cgcgtaaaca aggtcggctg cgtcgaaacg
    20101 taaaatccag cacgcgagac atacatcgcc tgttcaacca tcgacgagga gttgtgcgcc
    20161 agtcgcagca agtaaccaac ttccagtgca gcggtaattt cattgtcgag gctttcattt
    20221 tcgcgagaca ggaaattccc ctcgctgact aatgcatccg atacgccatt gactggcagg
    20281 gtgcgccgtc ggttgagttc gatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg
    20341 gttacggcat tacgcagaga ggtgaaatcc agtggcgcca cgagagcttc gcgcatgcca
    20401 ttacgcaaaa agatcagttt gtcgacgtta tactgcaaat gcttatcgag cgcgttggct
    20461 acgttttcca gatgattacg ctagctggag atataggcat cttccagcac caccacttca
    20521 cgccaggtga gcaaggtgga aaaaagcaat accacgataa agcagagatt aacgacatga
    20581 ccgggcccca ggcggcgtgc gagttttttc aaccagctct ggttttccat ttttgtctcg
    20641 tgctgcaccc tgacgactcc caacctgctt ttttatgatt ctggcataac ccggcaatag
    20701 cgtaccgcaa aacagcgata attttataat ggtggcatga taagacgcga ctatcattca
    20761 ttgcaggcat tgggacacca ggccggatgt gatccggcct ggtgttaatg ataccctttc
    20821 aaacttcttc cgcagaacga ctcccctccc ccggaacaag atcggtttca tgaaaagctt
    20881 cccgcttgac cccgtaaccg tcataccaac gacactcaac cataccgctg gagtatccag
    20941 tgacaatcat ccgcgggccg ccctctttaa ccgtaacttc ctcactaacc ataaagctca
    21001 tactcgcctc cttttttcga gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc
    21061 ataatagcca gtgcgagtat taatgtgcct gaccgcgcat tttcgctact gctttcactc
    21121 ccagcaccac gatgccgccg ataataaatc caagaatcag atttaaaaca gtcggtaata
    21181 tcatcgccac cactgcactt tgctgcccgg cgatatgttc aatagcgtga tgcagcggcg
    21241 caataccatg taccacaatc ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg
    21301 ataatgcttt catcagccaa ggcgcgataa tcaataaccc tttacctaat gcctgcatca
    21361 gcgcgctaga tttttccgcc agccaatacc ctaggtcatc aatcttaacg ataaccccta
    21421 ccagaccgta gacgcccacg gtcactacca gcgcgatgcc tgaaagcacc agcacctgat
    21481 taagcaacgg cgcctctgcc acaatcccca gcgtgatggc gacgatttcc gcagacaaaa
    21541 taaaatcggt acgaatcgcc cctttaattt tgtccttttc aaacttcagc ggatcctgcg
    21601 ccgccagctt ctccagacgc tgctggctct gcgctggatc ttctttatgt ttacgcgcct
    21661 ccagcatatg cagcactttc tctacccctt caaagcagag aaacgcgcca ccaatcatca
    21721 acagtggcgt aatcgcccac gggataaacg cactgatgat cagcgccagc ggcaccagaa
    21781 tcactttatt gatcagcgat cctttcgcca cgccccagac cacgggaagt tcccggttgg
    21841 cccgcacgcc tgaaacttgt tgcgcattga gcgataaatc atcccctaat acaccggcgg
    21901 ttttcttcgc cgccagtttg cccatcacag agatatcgtc cagcagtgtg gcgatatcat
    21961 cgagcaacgt cagtaaacta ctcccggcca aaagcattcc ctctcatttt tattgttgaa
    22021 gggtgtaagt atgaagcaaa aacggcagct ctgatacttg cttcacaggt caacatttat
    22081 ttaacaatca agtgcaatta tatctctcgc cagcagaatg attttcacgt ttactatgaa
    22141 ccaccttttt atttccgccg tgagggatta tgcgtttccg ccagttgtta ccgctttttg
    22201 gcgcgctgtt tgcgttgtat atcatttggg gctcaaccta ttttgtcatt cggattggcg
    22261 tgggaagctg gcctccgtta atgatggcgg gcgttcgatt ccaggcagcc ggtattttat
    22321 tgctggcatt tttgctactg cgcggacaca aacttccccc gctacgtccg ctgctcaatg
    22381 ccgcgctgat tggcctgtta ttgctggctg tcggtaatgg catggtgacg gttgccgaac
    22441 atcaaaatgt tccttccggc atcgccgccg tagtgattgc aaccgtgccc ctctttaccc
    22501 tgtgcttcag tcgcctgttt ggcattaaaa cgcgcaaact ggaatgggtg ggtattgcca
    22561 ttgggcttgc cggaatcatc atgctcaata gcggtggaaa tttaagcggc aatccgtggg
    22621 gcgcgattct gattttaatc ggctcgatta gctgggcgtt tggctcagtt tatggctcgc
    22681 gcattacctt acctgtaggg atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg
    22741 tgttaatgat cgcatcgatg attgcgggtg aaaaactgac ggcgctccct tccctgtcag
    22801 gcttccttgc ggtcggctat ctggcgctgt ttggttcgat tatcgccatt aacgcttata
    22861 tgtatttaat ccgtaatgtc agtccggctc tcgccaccag ctacgcctac gttaacccgg
    22921 tggtcgcggt cttgctgggt acgggactgg gtggcgaaac gctgtcgaag attgaatggc
    22981 tggcgctcgg cgtaattgtt ttcgcggtgg tactggtcac gttgggaaaa tatctcttcc
    23041 cggccaaacc cgttgtcgcg ccagttattc aggatgcatc aagcgagtaa atgaatcccc
    23101 tgcgtgtcga tctgcgcgct ggcaccttcg ccgcagatcc actcttccag acgctcggta
    23161 agcgccgcat ccgtcagctt ctcgcgccta cgtaacgcac actcccagac aatcagtacg
    23221 cgccagccga gctcctgcaa gcgactgata tcgcggcgat cgcgttcaac atttttacct
    23281 atcttctcca gccagaactc agttcgagtc gcaggcactt taaacagata gcagtgatga
    23341 tgatgccaga agcagccgtg ggtaaatatc acgcagcgat attcatcaac gacaaaatcc
    23401 gggcgtccgg gcagactggc gtcctgaacg cgaaacgcaa ggccctgccc ggttaacaaa
    23461 ctggcgaggc gcttctcaat cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg
    23521 cgggtggcct tatcgtgaac gtcgaccatg ttgtgcctct tgctgacgca acgccaccgc
    23581 ctgtttgatt tttggctcaa gcagttttgc caccgcggca aagaccggca cgaccaccga
    23641 gttgccgaac tggcgatagg cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc
    23701 cggcgcttca aaacccatta agcgcgcgca ttcacgaggc gttaaccgac gtggacgatg
    23761 ttgctgattc agcggatcgt caaagtcttt ctcacccgtg gccatatccc aaccgcgatc
    23821 gattaaaatt tccgcgccat ctttgtaata acgagcagac agcgtacggg tgacgctttg
    23881 cggattgttc ggataaacca ttccataacc gaagccgtta ccgcgcgcct gatgtttttt
    23941 cgcatatcga tagaggtact tccacagcac cggcgtcagg atatatttcg cctcaaccat
    24001 cgggtccagc agctgcgcca gcgtcactcg ctgtgcaggg aaacattcgc tgatatcacg
    24061 cagagtaaaa tcggctttca gattcagatc gcgacgaaaa cccaccagca cgatgcgttc
    24121 acggtgctgc ggcaggaaat gtttaccatc gatgattttc ggatcgtccg gcccgttatc
    24181 ttctgcatcg gcaacgtcat agcccagttc gtccagcgtc tgcatgatga tgcggaacgt
    24241 ttttccctta tcgtgactct tcaggttttt gacgttttcg agcacaaaca tcgccggacg
    24301 acgcgcgtcg ataatgcgca ccacatcaaa gaacagcgtg ccctgggtat cgcaggcaaa
    24361 accgtgcgcc cgcccgagcg agttcttttt cgatacgcca gccagcgaaa atggctgaca
    24421 agggaaaccg gccagtaaaa catcgtgttc aggaatgtgt tgacgaatat gttccgccgc
    24481 cgcctcatca ctcacgcctt cttgatgact gagggtgatg tcgcggatat cttcattaaa
    24541 atgatgcgtc gccgggtcgc aataatggtt ggctttataa gtgcgtaccg catgtttgtt
    24601 ccattcgctg gtaaacacgc actgtccacc aatcgattca aaaccacgac ggatgccgcc
    24661 aattccggca aatagatcga taaagcgaaa cgcataatgc ggatgatgtt ccggtggttt
    24721 gggtaacaac gtttgcagat gggcaaactc tttctcactt aaacggtgcc atgccgagtc
    24781 attcgccagc gcacgtttta aaattgccgc gctccagtga ttctcaccca caccattaag
    24841 ctgcgccacc agcattttaa catcataaat ttgcagcagt ttctccagca ttgcctgtgc
    24901 ggcattcccg gtgctgtatg aatcggttac tgatatattt tcctgcatag atttaaccgg
    24961 ccatctgaga tggccgacag gttaacataa ttactgccgt ttagggccac gcgctcagcc
    25021 agcgggtgaa aacgcatcta tcaccttgtg atcgatacct tcattctccc ccgccagttc
    25081 ggcaccgagc ttcgccatca aacagcgcca agctcaatgc ccctgaaacg gcaaatacgc
    25141 gcgccaggcc aatcgccccc agggcttcca gccgatccgc atcctggacg attttagctt
    25201 ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg
    25261 cctcaatttt ctccgccgga aattgcacga actcttcacg gagcaggcga cgcgtctctt
    25321 ctgccgccag gattgaagaa cgctgccgtt gcggatgatt tttcgccagg ctcacgatat
    25381 cgtgaaaata acaggcggtt aaaatcacca gcatatcgac atcgtcatct gccgccagtt
    25441 tctgtgccgt agcccagaca cggcgaaaat gacacaggtc atgcgccgcg tcctgatgtt
    25501 gatgatggtt tttcaaccag ttctcgaact atgcctgcca gtgttgtaac tccatacgcg
    25561 ctccgttgcc aaagagtctc aaccttagca gtttttattc atagcgtctg caacgtgcgg
    25621 gacgaggtcg ggtgtaccat gcaatgccgc ctaacaaggc accaaccagg gccataaaca
    25681 gaaatccgac caatgctcct aaccacttcc ccgcggtaga tcctaaatca ctttcaaatc
    25741 ccgacacggg ggcatcgggg atccggctca tcacccagat gtatcgcgcc attgcccaga
    25801 cgaaatagcc acaccatgta tagaaagccc
//
LOCUS       DAJTPG010000054        25652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTPG010000054 DAJTPG010000000
VERSION     DAJTPG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO230_002308"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO230_002308"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5692038.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO230_002309"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO230_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5692039.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO230_002310"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO230_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5692040.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO230_002311"
     CDS             3807..3995
                     /locus_tag="OO230_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5692041.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO230_002312"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO230_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5692042.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO230_002313"
     CDS             4756..5115
                     /locus_tag="OO230_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5692043.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO230_002314"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO230_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO230_002315"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO230_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5692044.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO230_002316"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO230_002316"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5692045.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO230_002317"
     CDS             10913..11254
                     /locus_tag="OO230_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5692046.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO230_002318"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO230_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO230_002319"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO230_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5692047.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13676..14962
                     /gene="dctA"
                     /locus_tag="OO230_002320"
     CDS             13676..14962
                     /gene="dctA"
                     /locus_tag="OO230_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5692048.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15183..16679
                     /locus_tag="OO230_002321"
     CDS             15183..16679
                     /locus_tag="OO230_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5692049.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16775..17704)
                     /gene="kdgK"
                     /locus_tag="OO230_002322"
     CDS             complement(16775..17704)
                     /gene="kdgK"
                     /locus_tag="OO230_002322"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5692050.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17936..18703
                     /gene="pdeH"
                     /locus_tag="OO230_002323"
     CDS             17936..18703
                     /gene="pdeH"
                     /locus_tag="OO230_002323"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5692051.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18773..20833
                     /locus_tag="OO230_002324"
     CDS             18773..20833
                     /locus_tag="OO230_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5692052.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21015..22337)
                     /locus_tag="OO230_002325"
     CDS             complement(21015..22337)
                     /locus_tag="OO230_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5692053.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22748..23761)
                     /gene="yhjD"
                     /locus_tag="OO230_002326"
     CDS             complement(22748..23761)
                     /gene="yhjD"
                     /locus_tag="OO230_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5692054.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23810..24709)
                     /locus_tag="OO230_002327"
     CDS             complement(23810..24709)
                     /locus_tag="OO230_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5692055.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25229..>25652
                     /locus_tag="OO230_002328"
     CDS             25229..>25652
                     /locus_tag="OO230_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5692056.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKS"
BASE COUNT         6105 a         6445 c         6981 g         6121 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgccaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc cccccataac cttacaagac ctgtggtttt actaaaggac accctatgaa
    13681 aacctctctg tttaaaagcc tttactttca ggtcctgaca gcgatagcca ttggtattct
    13741 ccttggccat ttctatcctg aaataggcga gcaaatgaaa ccgcttggcg acggcttcgt
    13801 taagctcatt aagatgatca tcgctcctgt catcttttgt accgtcgtaa cgggcattgc
    13861 gggcatggaa agcatgaagg cggtcggtcg taccggcgca gtcgcactgc tttactttga
    13921 aattgtcagt accatcgcgc tgattattgg tcttatcatc gttaacgtcg tgcagcctgg
    13981 tgccggaatg aacgtcgatc cggcaacgct tgatgcgaaa gcggtagcgg tttacgccga
    14041 tcaggcgaaa gaccagggca ttgtcgcctt cattatggat gtcatcccgg cgagcgtcat
    14101 tggcgcattt gccagcggta acattctgca ggtgctgctg tttgccgtac tgtttggttt
    14161 tgcgctccac cgtctgggca gcaaaggcca actgattttt aacgtgatcg aaagtttctc
    14221 gcaggtcatc ttcggcatca tcaatatgat catgcgtctg gcacctattg gtgcgctcgg
    14281 ggcaatggcg tttaccatcg gtaaatacgg cgtcggcaca ctggtgcaac tggggcagct
    14341 gattatctgt ttctacatta cctgtatcct gtttgtggtg ctggtattgg gttcaatcgc
    14401 taaagcgact ggtttcagta tcttcaaatt tatccgctac atccgtgaag aactgctgat
    14461 tgtactgggg acttcatctt ccgagtcggc gctgccgcgt atgctcgaca agatggagaa
    14521 actcggctgc cgtaaatcgg tggtggggct ggtcatcccg acaggctact cgtttaacct
    14581 tgatggcaca tcgatatacc tgacaatggc ggcggtgttt atcgcccagg ccactaacag
    14641 tcagatggat atcgtccacc aaatcacgct gttaatcgtg ttgctgcttt cttctaaagg
    14701 ggcggcaggg gtaacgggta gtggctttat cgtgctggcg gcgacgctct ctgcggtggg
    14761 ccatttgccg gtagcgggtc tggcgctgat cctcggtatc gaccgcttta tgtcagaagc
    14821 tcgtgcgcta actaacctgg tcggtaacgg cgtagcgacc attgtcgttg ctaagtgggt
    14881 gaaagaactg gaccacaaaa aactggacga tgtgctgaat aatcgtgcgc cggatggcaa
    14941 aacgcacgaa ttatcctctt aatctcacca cttatgcccg cactcccttc cccgagtgcg
    15001 ggtgtttgcg cataattgac ccctgtgcct gaacttacca cctgggtgag ttcaggttta
    15061 tttcacttct tccgtgacat tttcatgttc ttgcggtcta acacgaagtg tttttaacgt
    15121 catttagact gaccgacaac gggcagtttt tttattacca ggatagttga tcaggggttc
    15181 acatgcaggg cataaaaatt cgacttttag cgggcggttt gctgatgatg gccactgctg
    15241 gctatgtgca ggcagatgcg ctccagcctg atccagcatg gcaacagggg acgctttcca
    15301 acggtttaca gtggcaagtg ctgaccaccc cccagcgtcc cagcgatcgt gttgaaattc
    15361 gcctgctggt taataccggt tcgctcgccg aaagtacaca acagagcggt tacagtcacg
    15421 ccatccctcg tattgcgcta acgcaaagcg gtggccttga cgcagcacag gcgcgttcat
    15481 tgtggcagca ggggatcgac cctaaacgcc cgatgccgcc ggtaattgtc tcttatgaca
    15541 ccacgctgtt taatctgagt ttgcccaata accgtaacga tttgctgaaa gaagcgctct
    15601 cttatctggc aaatgccact ggcaaattga ccatcacacc agaaaccatc aaccacgcgc
    15661 tgcaaagtca ggacatggtg gcaacctggc ctgccgatac taaagagggc tggtggcgct
    15721 atcgtctgaa agggtcaacc ttgttaggtc acgatcctgc cgatccgctg aaacaacccg
    15781 ttgaagcgga aaaaattaaa gatttctatc agaaatggta caccccggat gcaatgacgc
    15841 tactggtggt gggaaacgtg gatgcgcgct cggttgtcga ccaaatcaac aaaacgtttg
    15901 gcgaactgaa aggcaaacgt gaaacgccag ctccggtgcc gacgctttct ccgctgcgtg
    15961 cggaagcggt gagtattatg actgacgcgg tgcgtcagga ccggttatct atcatgtggg
    16021 atacgccgtg gcagccgatt cgtgaatcag ccgcactgct gcgctactgg cgtgcggacc
    16081 tggcccgcga ggcgctgttc tggcatgttc agcaagcgtt aagcgccagt gacagcaaag
    16141 acatcggtct tggatttgac tgccgtgtgc tgtatctgcg tgcgcagtgt gccatcaaca
    16201 tcgaatcacc aaacgacaag ctgaacagca accttaatct ggtggcgcgt gaactggcga
    16261 aggttcgcga taaaggtctg ccggaagaag agttcaatgc gttagtggcg caaaagaaac
    16321 tggagctgca gaaactgttt gccgcctatg cacgggctga taccgatatt ctgatgggcc
    16381 agcggatgcg ttcgttgcaa aatcaggtcg tcgatatcgc gccggagcag tatcagaaac
    16441 tgcggcagga tttccttaac agcctgactg tggagatgtt aaatcaggat ctgcgtcagc
    16501 agttgtcgaa tgatatggcg ttaatactgc tgcagccgaa aggcgagccg gaatttaaca
    16561 tgaaagcgtt gcaggcggcc tgggatcaaa tcatggcccc atctaccgcc gctgcgacca
    16621 cctctgtcgc cacggatgac gtacatcctg aagtgacgga tattccacct gcacagtaat
    16681 gcctgatggt gatgagttag cacaaccatc aggtatttat ggatgagctg gatagagggg
    16741 taacacttta tccagccttt tgcatatgct gcgtttacgc tggcatcgcc tcacgcggga
    16801 taatcgcgcc gcgatactga ataacggtac ttgcggtcag gtgcccgcgt ttcgccgcgt
    16861 tttccgcact gccgcccgtc aaacgtaccg ccagataacc ggcgctgaaa gagtcgccag
    16921 ctgcggtggt atcgatcact ttttctttcg gcagtttcac cgccggaaca tcaactaacg
    16981 cttcaccagc aatggacacc aggcaagaat ccgccccgcg tttcaccacc acttctttca
    17041 cgcccgcgtt atgggtgcgc gcaatgacgt cttccaccgg ctgttgaccc cacagcgcgt
    17101 cttcgtcgtc cagcgtcagg aaggcgatat ccgtgcattc cagcatttgc tggtacacct
    17161 gctgtgtctc ttctttgctg gcccacaggc gcggacgata gttattgtcg aaaatcactt
    17221 ttccgccttt ggcgcggcat tcgcgcagca gggaaagcag cttttcgcgg ctggtcgggc
    17281 ttaagatcgc caggctaatc ccgctcaggt agagataatc gaaattcgcc agctcttcgc
    17341 aaatcgccgc agactgctcg ctcgccagcc agaatttggc ggcggcttcg ttacgccagt
    17401 agtagaacgt acgttcgccg gtgctgtcgg tttcaatgta gtaaaggccc ggaagacggt
    17461 tttccatccg ttgggtcagg gaagtatcaa cgttctcgcc gtgccaggcg tccagcatct
    17521 gctggctaaa actgtccgtt cccagcgccg ttacgtaatg aacggttaat gccgcaggat
    17581 cgacctgacg ggcgatatag acggaagtgt tcagggtatc gccgccgaaa ccgcgcttaa
    17641 cgtccgcgcc tttctcggaa agctcaatca tgcattcgcc aatcacggca atctttttgg
    17701 acatagtcgt gaacctgatc tgtaaaaaat tagcgttagt ttgcgctgtg gtggtttgct
    17761 ggtcaaccat attaaaacag tgttccatta tttttttgag ccagaacgta ttcctgaaag
    17821 attccgttgt ggagtgagga aatttacccc ggccaggcgg taaagttctg cccttacgcg
    17881 ccgataatct ttgtcgagtc cgggcagcat cacttttaaa cacaggacat ctttgatgat
    17941 aaggcaggtt atccagcgaa taagcaaccc tgaagcaagc atcgagagct tgcaggaacg
    18001 gcgtttttgg ttgcagtgtg agcgtgctta cacctggcag ccgatctatc aaacatgcgg
    18061 gcggttaatg gccgtggagc tattaacggt ggtcacgcat cccttgaacc cttcgcaacg
    18121 cctgccgccg gatcgctatt ttactgaaat caccgtcagc catcggatgg aggttgtgaa
    18181 agagcagatt gatttgctgg cgcaaaaagc cgacttcttt gtagagcacg gcctgctggc
    18241 atcggtcaat attgatggcc ctacgctcat cgccctgcgt cagcaaccaa aaatcctgcg
    18301 ccagattgag cgtcttccct ggctgcgttt cgaactggtg gagcatatcc gtctgccgaa
    18361 agattcaacc tttgcctcga tgtgtgaatt tggcccgctg tggctggatg attttggtac
    18421 cgggatggca aatttctctg cactaagtga agtgcgttat gactacatca aaatcgcgcg
    18481 agaactgttt gtgatgctgc gtcagtcgcc ggaaggccgc acactctttt ctcagctttt
    18541 acatctaatg aatcgctatt gtcgcggggt gattgtcgag ggcgtagaaa caccggaaga
    18601 gtggcgtgat gttcagaact cgcccgcatt cgccgcacaa ggctggtttc tttcacgccc
    18661 ggcaccgatg gaaacgctga atacggcggt tctggcgcta taagctgcct cattttccgc
    18721 cgggctggac tatctttagg actggcacag gaagaatatg aggaagcgaa cgatgagcaa
    18781 ggcaggcaaa ataaccgctg cgatttcagg ggctttcttg ttgttgattg tcgtggcgat
    18841 cattttgatt gcaacttttg actggaatcg actcaaaccg accatcaacc agaaagtctc
    18901 tgcggagttg aatcgtccgt tcgctatccg tggcaatctg ggcgtggtgt gggagcggca
    18961 aaaacaagaa actggctggc gcagctgggt gccgtggcct catgtacatg cagaagacat
    19021 cattcttggt aatccaccgg atattcccga agtcacgatg gtgcatttgc cgcgagtaga
    19081 ggcaacgctg gcaccgctgg cgctgctgac caaaacggtc tggctgccgt ggatcaagct
    19141 cgaaaagccc gacgcgcgcc tgattcgcct ctctgaaaag aacaataact ggacgtttaa
    19201 tcttgccaac gatgacaaca aagacgcgaa tgcaaagccg tcggcatggt cgtttcggct
    19261 ggataatatt cttttcgatc aagggcggat cgccattgat gacaaagtaa gcaaagcgga
    19321 tctggaaatt tttgttgatc cgttaggcaa gccgctgcca ttcagcgaag ttactggatc
    19381 gaaaggtaaa gcggataaag aaaaggtggg cgattacatt tttggcctga aggcgcaggg
    19441 acgttataac ggtgaaccgc tcacgggtac gggaaaaata ggcggtatgc tggcgctgcg
    19501 tggcgaaggg acgccgtttc cggtacaggc tgatttccgt tcaggtaata cccgtgttgc
    19561 ttttgatggc gtcgtgaatg acccaatgaa gatgggcggt gtcgatttac ggcttaaatt
    19621 ttctggcgat tcactgggtg atctctatga actgacgggc gttctgctgc ccgatacccc
    19681 gccgtttgaa acggatggtc ggctggtagc gaaaatcgac actgaaaaat cgtcggtctt
    19741 tgattatcgc ggttttaatg ggcgaattgg cgatagcgat atccacggtt ctctggtcta
    19801 caccaccgga aagccacgac caaaactgga aggtgatgtc gagtcgcggc aattgcggct
    19861 ggcggacctg ggaccgttga ttggcgttga ttccgggaaa ggggcagaaa agtcgaaacg
    19921 gtctgaacag aagaagggcg aaaaaagcgt tcagcctgcg ggcaaagtgc tgccttatga
    19981 ccgcttcgaa accgataaat gggacgttat ggatgccgat gttcgcttca aagggcggcg
    20041 cattgagcat ggcagtagcc tgccgattag cgatctttct actcatatca tcctcaaaaa
    20101 tgctgacctg cgcctgcaac cgctgaaatt tggcatggcg ggcggcagca ttgcggcgaa
    20161 tattcatctg gaaggcgata aaaagccgat gcaggggcgg gcagatattc aggctcgtcg
    20221 actgaaactg aaagaactga tgcccgatgt ggaactgatg cagaagacgc tgggggaaat
    20281 gaacggtgac gcggaactac gcggtagcgg taactcggtg gcggcgcttt taggcaacag
    20341 taacggcaac ctgaaactgt tgatgaatga cgggctggtg agccgcaacc tgatggagat
    20401 tgttgggctg aatgtcggca actacattgt cggtgcgata tttggtgacg atgaggtgcg
    20461 ggtgaactgc gcggcggcga atctgaatat tgccaacggc gtggcgcgcc cgcagatttt
    20521 tgctttcgat actgagaacg cgttgattaa tgttaccggc acggcaagtt ttgcttcgga
    20581 acagctggat ttgactattg atccggagag taaagggatt cggattatca cactgcgttc
    20641 gccgctgtat gtgcgtggga cgtttaaaaa tccgcaggct ggggtgaaag ccggaccgct
    20701 gattgcccgt ggtgctgttg ctgcggcact ggcaacgctg gtaacaccgg cggcggcgtt
    20761 actggcactg atctcacctt ccgaagggga ggctaatcag tgtcggacga tattgtcgca
    20821 gatgaagaag tgatgataat tgccggatgt ctgacatccg gcaattaaac agatttagat
    20881 ttttggcaaa gtgtgctttg ttcatgccgg atgcggcgtg aacgccttat ccggcctaca
    20941 aatccttgtg aattcaatat attgtatgga aattcagtcc tgataagcgt agcgcatcag
    21001 gcttttcaca cttattacaa cgactgatgt cgcgtctcat gggtcagcag cagggcgatt
    21061 aacgtcaagc cagccatcgc cgccagatat aaccccaccg cacctaaccc gtagttagcc
    21121 tgcaaccagg ctgcgatata cggcgcaacg gaagccccga gaatcgacga tacgttgtaa
    21181 gagaacgatg ctccggtgta acgcacttct gtcggaaaca gctctggtaa cagcgcaccc
    21241 atcggcccga aggtcagccc catcagactt aaccccagca gcaggaaggc aaaaaccaga
    21301 atcgggttgc cagaaccgag cagcgggtta aaggcgaaca gcgcgaacag gatgatcagc
    21361 gtggtgatga tcaccatgct tttacggcga ccaaaggcat cagccagtaa tccggcgact
    21421 ggcaccatca cgccaaaacc aataactgcc atcatcagca tccacaacac ttcgttacgc
    21481 ggcaggccaa gcccaactgg cgcggcggcg gtactgaagg tcatagagta gaccgtcatg
    21541 atgtaaaaca gcgtataggt tgccagcata atgaacgtac ccagtacggt tacgcgaaca
    21601 tgtttggtca gcagcgtacc cagcgggatc ttcacctgtt ttttcgcttt agcgactttc
    21661 tcaaacaccg gcgactcatg cagcgacacg cgaacataca ggccgataat gaccagcacc
    21721 gccgagaaga taaatggcac acgccagccc cagctcataa actgctcatc ggtcagtagc
    21781 caggaaagca gcaagaaagt gccgttggca aagaagaagc caatcggtgc gcctagctgt
    21841 gggaaggagc catacagtgc acgtttgcgc ggtggggcgt tttcagtcgc cagcagcgcc
    21901 gcgccgcccc attcaccgcc taagcccaga ccctgaccaa atcgagccag cgccagcagc
    21961 agtggggcga aaataccaat cgtggcatag cccggcagca gaccaatcac cacggtcgaa
    22021 atccccatcg ttagcaacga ggcgaccagc gtcgctttac gcccaacgcg atcgccaaaa
    22081 tgaccaaaaa cggcagagcc aatggggcgc gcgacgaagg cgatggcgaa ggtggcgagc
    22141 gactgtagcg ttgctgccgt tggatcgccc tgcgggaaga agatatgcgg aaacacaata
    22201 acggccgcag tggcgtaaat gtaaaagtcg aagaactcaa tggccgtgcc aatgagagag
    22261 gcgacaagga ctttattacg cgagttgatc ggcgtgtatt cttgttcgtg gtcgagtgtt
    22321 gtggctgttg cttgcataat cttttcttat tttttggcta acgaatagcc atattacgca
    22381 cagcaaaagt gatatttcaa tgtgtaacaa atcgcggaaa tggtcaaaaa ccaggcaaaa
    22441 aggggataat gtttaggcca gtgatttttc ttctatgaaa tgcttcacat aattttataa
    22501 atagtggata aaactggttt caggttaaat aaaagttacc aactggattt atatgctggg
    22561 gttatgagtt ggagtgaaat tgtttgctcc caaatagatg gctgaagagg ctctggggaa
    22621 gagtttcaag aggttatgtg cgcattatag ccggatgcga tgctggcgca tcttatccgg
    22681 cctacgggga gccaggccga ataaaatcgt atgccggata aggcgtttac gccgcatccg
    22741 gcctatttta aggctgcgtt ttccctggca ttcgcgggtc gtctttatat tcggcggtgg
    22801 caatccacgc cgcgcaaaac agcgtcaaac gggcgaagaa gtagaaaaat gccatcagcc
    22861 ccagcacgga accaaatgct gcgccagagg gggatttcat cagcgatggc agggtgtagg
    22921 tcatcacgat tttaatcact tcaaaaccaa tagcagccag aaatgttccg cgaatcaggg
    22981 cttttttgcg cggacggtgg cgcggcaacc gccagaagat ccagaagaaa agcagatagt
    23041 tggcgaagat ggaaatcgcc aggccaatca atcgccacgt cggtttcagc cactcaatgc
    23101 tgttcaggtg cagggcgcta ataatcattt gctgcgccga accggcaacc gaggtgatcg
    23161 aaagcgtcac aatcagcgca atcaacaaac caatcagtga aataaaatca cgcagatatt
    23221 ttacccagaa cttctcctga tcttgcggcg agcgttccca gacatcgcgc gactgggcac
    23281 gaatcgcttc acgcaggtta cccatccagt tgatgccgga ataaagtgcc accgccaggc
    23341 cgacaagccc tacagtcgta cgctgctgaa cggcggtgtt gatggtgttt ttcaacgtgg
    23401 cggctagtgt cggatcgctg atgttttgca gaattttgtc gaagatatcc tgtagcaaca
    23461 tcggatggga ggccagcaca aagccccctg cggcaaacga caccatcaaa atcgggatca
    23521 tcgacaaaaa tgagaaatag gtgatagccg caccaaactg gttacccagc cgatcgttaa
    23581 agcgttctgt cgcgcgaata aggtgcgcaa taaccggttg gcgttggact ttttcggcag
    23641 tggtggtgac ggtttccagc gcctggctaa ctttgccgcc tttctcgcta ttatccaact
    23701 gcttaatcgg ctcgtgctcc agatcctggg tgggacgttt gatctcgttt tcctgcgtca
    23761 tccgctcttt tgtccttctg tcgtggtcat tcttaaaaag tatagtcggt cagtccaggt
    23821 gctctttcat tacgccagcc agccactgca taaacaggtt tacacgccgg gaaagctccc
    23881 gacgctgcgg gtaaaccagc gaaagggaga gcggctcggc acggtagcct ggcaacactt
    23941 caataagccg cccggcacgc agggcttcgc gcacggcaat gcgcgggatc tgaataatcc
    24001 ccagaccggc aagaccggcg gcgagatagt tttcgctact gtttaccgtc aacatgccgc
    24061 cagacttaaa ccactggacg ccattaacgc tggcaacctc aaaacctaac ggatgtacac
    24121 ccaggtgcgg tgtataacgc actattgcgt gtgaagtcag atcatcgggg ctttgcggat
    24181 aaccaaagcg cgtcagatag tgcggactgg cacagttgac catggttagt ttgccgaggg
    24241 gacgggcgat aacgccatct tccggtaacg cgccagtgcg tatcacgcaa tcaaaaccat
    24301 catgaagaat atctaccgga cggttatggc tactcagttc cagctcaatt cccggatgct
    24361 gatagagaaa ttccgacaga cgcggcagta acaggttttt cgcgattcct gacgggatgt
    24421 cgatgcgtaa tttaccgctg atactggtgg catcctgttg aaacagaccg tccagttcgc
    24481 tgagattact caacacatct tttgcccgtt gataataggt catgccttct ggcgtgagtt
    24541 tgacccgtcg cgtagtgcgc tggagaagct gggtgccaag ttgatgttcc agtgcctgta
    24601 tctggcgcga aacacttccc tttggcaaag caaagaaatc cgccgcgcgg gaaaaacttt
    24661 ccagctccgc gactttgatg aacaactgca ttgcgtgaat tttatccatt tctttgaccg
    24721 attgttgttc acagcgaaac agtaacgcgc atatcgcatt gtttattgtt ttttctaaca
    24781 tctaatgccg ggtttcacaa tgttgtcatg acgaatgtgg cgatttctca gcaggaaaag
    24841 ggcggaatag ctttatgaga aaggtgaatc cccttaaaca gagcgtattt ttcagtgatt
    24901 ccctttaaag tgaatagagg acgtattggc tatttgtgct tatttttgtc tcattgccat
    24961 ttgttatttt ggcttgtagt cgcggaattt catttttaat tgcatgcgta ctaaccacca
    25021 atataaggaa atcctcaaaa ttgttttaaa tagtgccctc gacatgtaat ttcaggcgtt
    25081 tagctgtaac tatttatatc ggtaactatt ttctacgcgt catctctgta gaatggccac
    25141 cgccaaatgg cgaattctta ttttttctcg cgggtaatac ccttccacta cttaagtctt
    25201 tttaacgtat tcaggtggag attcagttat gcaaatagtc atgtttgaca ggcagtcaat
    25261 atttattcat ggaataaaaa tcagtttaca gcagcgtatt ccaggagtga gtattcaggg
    25321 ggtcagtcag gcagacgagt tatggcaaaa gctggaaagt taccctgaag ccttagttat
    25381 gctcgatggc gatcaggatg gtgagttttg ctactggttg ctgcaaaaaa ccgtggtgca
    25441 gtttcctgag gttaaggtgt taatcacggc gacggattgc aataaacggt ggttacagga
    25501 agttatccat tttaatgtgc tggccattgt gcctcgcgat tcaaccgtcg agacatttgc
    25561 gttggcggta aatagtgcgg cgatggggat gatgtttctg cctggagact ggcggacgac
    25621 gccggaaaag gacattaaag atctcaaatc gt
//
LOCUS       DAJTPG010000055        25229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTPG010000055 DAJTPG010000000
VERSION     DAJTPG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(20..1384)
                     /gene="yeaG"
                     /locus_tag="OO230_002329"
                     /pseudo
     CDS             complement(20..1384)
                     /gene="yeaG"
                     /locus_tag="OO230_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
     gene            1820..2566
                     /gene="mipA"
                     /locus_tag="OO230_002330"
     CDS             1820..2566
                     /gene="mipA"
                     /locus_tag="OO230_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5692057.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2656..3417
                     /locus_tag="OO230_002331"
     CDS             2656..3417
                     /locus_tag="OO230_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5692058.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3468..4352)
                     /locus_tag="OO230_002332"
     CDS             complement(3468..4352)
                     /locus_tag="OO230_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5692059.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4436..5431)
                     /gene="gapA"
                     /locus_tag="OO230_002333"
     CDS             complement(4436..5431)
                     /gene="gapA"
                     /locus_tag="OO230_002333"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5692060.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5773..6186
                     /gene="msrB"
                     /locus_tag="OO230_002334"
     CDS             5773..6186
                     /gene="msrB"
                     /locus_tag="OO230_002334"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5692061.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6228..6500
                     /locus_tag="OO230_002335"
     CDS             6228..6500
                     /locus_tag="OO230_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5692062.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6870..7946
                     /locus_tag="OO230_002336"
     CDS             6870..7946
                     /locus_tag="OO230_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5692063.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7973..9352
                     /locus_tag="OO230_002337"
     CDS             7973..9352
                     /locus_tag="OO230_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692064.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9369..10414
                     /locus_tag="OO230_002338"
                     /pseudo
     CDS             9369..10414
                     /locus_tag="OO230_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10435..11271
                     /locus_tag="OO230_002339"
     CDS             10435..11271
                     /locus_tag="OO230_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5692065.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11276..12223
                     /locus_tag="OO230_002340"
     CDS             11276..12223
                     /locus_tag="OO230_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5692066.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWELNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12233..13213
                     /gene="ydjG"
                     /locus_tag="OO230_002341"
     CDS             12233..13213
                     /gene="ydjG"
                     /locus_tag="OO230_002341"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5692067.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13350..14108
                     /locus_tag="OO230_002342"
     CDS             13350..14108
                     /locus_tag="OO230_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5692068.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14226..15584
                     /locus_tag="OO230_002343"
     CDS             14226..15584
                     /locus_tag="OO230_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692069.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15677..16318)
                     /gene="pncA"
                     /locus_tag="OO230_002344"
     CDS             complement(15677..16318)
                     /gene="pncA"
                     /locus_tag="OO230_002344"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5692070.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16329..17345)
                     /gene="ansA"
                     /locus_tag="OO230_002345"
     CDS             complement(16329..17345)
                     /gene="ansA"
                     /locus_tag="OO230_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5692071.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17512..19368)
                     /gene="sppA"
                     /locus_tag="OO230_002346"
     CDS             complement(17512..19368)
                     /gene="sppA"
                     /locus_tag="OO230_002346"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5692072.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19529..20080
                     /locus_tag="OO230_002347"
     CDS             19529..20080
                     /locus_tag="OO230_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5692073.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20197..21240
                     /gene="selD"
                     /locus_tag="OO230_002348"
     CDS             20197..21240
                     /gene="selD"
                     /locus_tag="OO230_002348"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5692074.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21245..23206
                     /gene="topB"
                     /locus_tag="OO230_002349"
     CDS             21245..23206
                     /gene="topB"
                     /locus_tag="OO230_002349"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5692075.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23333..24373
                     /locus_tag="OO230_002350"
     CDS             23333..24373
                     /locus_tag="OO230_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5692076.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24490..25218)
                     /locus_tag="OO230_002351"
     CDS             complement(24490..25218)
                     /locus_tag="OO230_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5692077.1"
                     /translation="MRPEATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIE
                     KAMEFGARVITASDSSGTVVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEG
                     QQPWSVPVDIALPCATQNELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVL
                     FAPGKAANAGGVATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQT
                     NYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         6412 a         5850 c         6514 g         6453 t
ORIGIN      
        1 tccgctattg ccagtacacc tcaatctgct gttccaggac gtagaacaga tggaccgggt
       61 ttgccgccac ttctacatga tcgaagttga acacgcggga gaggatctta aacgcaaaac
      121 gcgtcgacag accgttcatc ccttcatcga caccggcgta gtcacgatat tcctgatacg
      181 acttggcttt cggatcggtg tctttcagac tttcgccatc ataaacccgc atctttgaat
      241 aaatgctgga gttttctggc tctttcaggc gcgaaagaat ggaaaaacgt gacagtgttt
      301 cgagcgtgcc aggggcgcat ggggcgtgag tcaattcact gtgattaagc aatttctcgt
      361 agattttgat ctcttcggaa atgcgcaagc aatacggcac cttcacgatg taaacacggt
      421 cgaggaaggc ttcgttgttt ttgttattac ggaaagtgac ccattcggac ttgttcgagt
      481 gggcgagaat aatcccgttg aacggcaggg cggagatacc ttccgtcccg ttgtagttac
      541 cttcctgagt ggcggttaac aagggatgca gcacttttat cggtgcttta aacatctcaa
      601 cgaattccat gatcccctga ttggcgcggc acagcgcacc ggaatagccg taggcgtccg
      661 ggtcattctg cgcgtagtgt tcgagtttac gaatatcgac tttcccaacc agcgcggaga
      721 tgtcctggtt gttctcatca ccgggttccg ttttggcgat agcaatttgt tgcagaattg
      781 acggccagac cttcactacc cggaacttag tgatatcgcc accaaattca tgcaggcgtt
      841 ttgccgccca tggcgacatg atggtgccga gataacggcg aggaatgcca tactcttttt
      901 ccagaatctg agcatcttcc tgcggattga aaagacagaa cggatgatcg ttgaccgggc
      961 tacgctcacc gttggcgctc aatacataaa tcggtacgag ctgcattaat gatttcagtc
     1021 gctcagcaag cgatgattta cccccaccca caggccccag cagatacagg atttgtttct
     1081 tctcttccag cccctgagcc gcgtgtttca gataagagac aatctgttca atcgcgtctt
     1141 ccatgccgta aaactcttca aatgccggat aacgtgcaat gacccggtta gaaaagagtc
     1201 gagaaagtct ggggtcctgg gctgtatcga ccatgacagg ctcaccgata gccatcaata
     1261 gccgctcagc cgcgttggca taagcactgc gatcttgccg acaagtggta agaaactcct
     1321 gcagtgtgaa ctcttcgtcc ttggcagctt catatcgctg gcgatagtga tcgaatatat
     1381 tcatggtatg ccgtcctttc gttttttagc acaggttaag agccgttcat atgaatagta
     1441 gaggctcccg gaagaggtaa actgaacgac gtctcttgct aaaacacagc aactctcatg
     1501 ccattttatc gacgatgaga acaacgtgcc cggtgataca ccttcaaaat taagcgtaga
     1561 tggcaaatgg aaaacttgcc tgcacggctt tcttaatttc aatggcatat caacaactca
     1621 tccataaatt ttgcataatt aatgtaaaga ccaggctcgc cagtaacgct aaatccattt
     1681 ggctgtaagc gcggtgtcat ccgcggcagg aaaattaaac agttacttta aaaaatgaaa
     1741 acgtaaaaag gttgggtttc gatgtattga cgggtaaact ttgtcgcccg ctaaacattt
     1801 gtttaaggaa tgattaattg tgaccaaact caaacttctg gcacttggag tgcttatcgc
     1861 aacgtctgca ggcgtagcgc acgctgaagg taaattttcc ctgggcgcag gcgtaggtgt
     1921 cgttgagcac ccatataaag attacgatac cgatgtttac ccagtaccgg taatcaacta
     1981 tgaaggcgat aacttctggt tccgtggctt aggtggtggt tactacctgt ggaatgacgc
     2041 aacggataaa ctttcaatta ccgcttactg gtcgccgctt tacttcaaag cgaaagacag
     2101 tggcgatcac caaatgcgtc acctggatga ccgtaagagc accatgatgg ctggtctgtc
     2161 ttatgctcac tttacccagt acggttacct gcgtaccacc ctggctggcg ataccctgga
     2221 taacagcaac ggcattgtct gggatatggc ctggttgtat cgttacacca acggtggcct
     2281 gaccgtgact ccgggtattg gtgtgcagtg gaacagcgaa aaccagaacg aatactatta
     2341 tggcgtatcg cgcaaagagt ccgctcgcag cggtctgcgt ggctataacc cgaatgacag
     2401 ctggagccct taccttgagc tgagcgccag ctacaacttc ctcggcgact ggagtgttta
     2461 cggtaccgcg cgctacaccc gtctgtctga tgaagttact gacagcccga tggtggataa
     2521 atcctggact ggcctgattt ctaccgggat cacctacaaa ttctgataat gcatctttcc
     2581 agggcatttt gtgcataaaa acagggcgtc acgccctgtt ttgcattacg gtggtgcaat
     2641 acctgggagg aaacaatgca acaaaaaatg attcaattta gtggcgatgt ctcactgcca
     2701 gccatagggc agggaacatg gtatatgggc gaagatgcca gtcagcgcaa aacagaagtt
     2761 gctgcactac gcgcaggcat tgaactcggt ttaaccctca ttgataccgc cgaaatgtat
     2821 gccgatggcg gtgccgaaaa ggtggttggg gaagcattaa ccggtctgcg agagaaggtc
     2881 tttctcgtct ctaaagtcta tccgtggaat gctggcgggc aaaaagcgat aaatgcatgc
     2941 gaagccagtt tacgccgtct caatactgac tatctcgatc tctacttatt acactggtct
     3001 ggcagtttcg cttttgaaga gactgttgct gcgatggaaa aattgatcgc ccagggaaaa
     3061 atccgtcgct ggggcgtttc taaccttggt tatgccgata tgcaggaact ctggcagctg
     3121 ccggggggaa atcagtgtgc cactaatcag gtgctttacc atctcggttc acgagggatt
     3181 gagtacgatc tactcccctg gtgccagcaa cagcagatgc cggtgatggc ttacagtcca
     3241 ttagcccagg ccgggcggtt gcgcaatgga ctgttaaaaa acgcggtagt caacgaaatt
     3301 gcacatgtcc aacaacatgc agctgcgctt gaggtcgaac tttcgtcagc ggaattagct
     3361 atgctggata aggcatatcc ggcaccaaaa ggaaaaactg cgctggatat ggtgtgatgt
     3421 tgatacgtgc cggatgcatc gcacccggca cacaacatta acgcaaatta acgtttggca
     3481 acgcgaatgg attgcgccag atgtgcaggt ttctctttgg tgactttttg cgtttctgaa
     3541 gcgtaagccg tttctacaca aacaaaggtc ttgtagccat cgtccggcat gtcgcccatg
     3601 ctaactgaaa gcgccggtcc cgggttccag ccgacaacgt tcagatgatg ctggtggcct
     3661 acggcgataa tacgattcag cgcttcatca ttaatcacgc tgcaatcttg cggattcaga
     3721 tacacgcgat cggtacggtc agggaaggtc tgaataccgt cggtcagtac atcttctttc
     3781 gcgtcattca ctttatcaat gaagcgatcg cccagcccac tgacgcttac cttagcgata
     3841 tcacccacgt taaagtaggt atgcagggca gaggtggttt caaattcgcc atgtgattca
     3901 agatcgattt cacaggtttt acccacgcgg aaatgcgcta acagcgtaaa gtcgtgcggc
     3961 cagaattttt tcgtctcttc gctttgtgtc aattcaaaag tcagcgctac gccatcagca
     4021 tcttcatgat gtgatttcag cgtccacggc aggttgcggg caaaaccgtg cgcaggcaga
     4081 ccttgttgtg ccgccggacc aaaccacggc cagcaaaccg gtacgccacc gcgaatagcg
     4141 acgccatttt tgaacggtgt gttgttgctc aaccacagaa cttcttcttc acccgcaggt
     4201 ttccacgaga gaaggtgtgc gccctgtaat gcaaaagagg cttttacctg gggatgatcg
     4261 accacaatga ggtccagttc atccagttta cgacgggaga ggacagggga gatttgttcg
     4321 atgaccggaa gggcaaaaat tttcttaatc atgacgcagt cctttaactt cattttatca
     4381 ggtaaaaaaa agagcgaccg aagtcgctct ttttagatca cagtgtcatc tcaacttatt
     4441 tggagatgtg agcgatcagg tccagaactt tgttggagta accggtttcg ttgtcgtacc
     4501 aggataccag tttcacgaag ttgtcgttca gagcgatacc agctttagca tcgaacacgg
     4561 aagtgcaaac ttcgccgttg aaatcggtag atactacgtc atcttcggtg tagcccagaa
     4621 cgcctttcat ttcgccttca gcagcagctt taacggcagc tttgatctgc tcgtaagttg
     4681 cagctttttc cagacgaacg gtcaggtcaa ctacggatac gttcggggtc ggaacgcgga
     4741 acgccatacc agtcagtttg ccattcagtt ctggcagtac tttacctaca gctttagcag
     4801 caccggtaga ggacgggatg atgttctggg aagcgccgcg gccgccgcgc cagtctttgt
     4861 gagacgggcc atcaacggtt ttctgagtag cggtagtagc gtgaacggtg gtcatcagac
     4921 cttcgatgat gccgaagtta tcgttgataa ctttagccag cggagccagg cagttggtgg
     4981 tgcaggaagc gttggaaacg atgtcctggc cagcatattt gtcgaagtta gcgcctttaa
     5041 cgaacatcgg agtgttgtct ttagacggac cagtcataac cactttcttc gcaccagcgg
     5101 tgatgtgttt acgagcagtt tcgtcagtca ggaacagacc agttgcttca gcgacaacgt
     5161 caacacccac ttcgtcccat ttcaggttag ccggatcacg ttcagcggta acacggattt
     5221 ttttaccgtt aacgatcaga tgaccgtctt tcacttcaac ggtaccgtcg aaacggccgt
     5281 gagtggagtc atatttcagc atgtatgcca tgtaatcagc gtctaacagg tcgttgattg
     5341 caacgatctc gatgtcagaa cgtttctgag cagcacggaa aacaatgcga ccgatacggc
     5401 caaaaccgtt gatacctact ttgatagtca tatattccac cagctatttg ttagtgaata
     5461 aaaggttgcc tgtaaaatta caaaaacctt acgcagcgtc aagcggaatc gtgtcaatca
     5521 ttgcgacaaa tcaatcctgt gcctaagcat tacgcgactg actcgcctca cttttccttt
     5581 gggcttgaga ccacatgggg tcggcgcccc gaattttaaa gggcaattac gataaaaatg
     5641 tgattttcat cacgatttag gtgcagccat ttcgtcgaga tcaagtttag gacaaaattt
     5701 tgccagttgg tgttaatgtt ttgttagaat cagtcacgta atgtgagcac gattaaagtg
     5761 agatgtgagt aaatggcgaa taaaccttcg gcagaagaac tgaaaaaaaa tttgtccgag
     5821 atgcagtttt acgtgacgca gaatcatggg acagaaccgc catttacggg tcgtttactg
     5881 cataacaagc gtgacggcgt atatcactgt ttgatctgcg atgccccgct gtttcattcc
     5941 gaaaccaagt atgattccgg ctgtggctgg cccagtttct acgaaccggt gagtgaagaa
     6001 tccattcgtt atatcaaaga cttgtcacat ggaatgcagc gcatagaaat tcgttgcggt
     6061 aactgtgatg cccatctggg gcatgtcttt cccgacgggc cgcaaccaac aggcgaacgt
     6121 tattgtgtta actctgcttc tttacgcttc actgatggcg aaaacggcga agaaatcaac
     6181 ggttgaaaaa acgattcagc aacttattcc acaggagcgg aaaaattatg aatcttgatg
     6241 acattatcaa cagcatgacg cctgaggtat accagcgttt gtcgaccgcc gttgaactgg
     6301 ggaaatggcc tgatggcgtt gcgttgaccg aggaacaaaa agagaattgc ctgcaactgg
     6361 tgatgctgtg gcaagcccgc cataataccg aagcacagca tatgacaatt gacacaaatg
     6421 gtcagatggt catgaagagc aaacagcagt taaaagaaga ttttggtatc tcagcaaagc
     6481 ctattgccat gtttaagtaa gtggttcatc ccctttttgc cgcggctatg aaggggatag
     6541 ccaacaatga ccccacgact cgtctcaatt atctcgaccc gacctggcta agtcccagtc
     6601 cgatctcttg cagtcgcgtt cacgcctgaa tgacttctcc tgaacttaag tctcagacct
     6661 atttggccgg taatccctct cgaatttgct ctttttgcgt cccgccttcc accaacctgt
     6721 aacagaccca gccattttct gcgtaattgt gatttgagtt gtaaaatgca ggtcattatc
     6781 tgtgttgtgc agatttaata ttgacctcaa aaatcatgtg ggtaagtatg atggccatct
     6841 aagcaaaaca aaacacagag gatgacgcaa tgaaagcact ggctcggttt ggcaaggcct
     6901 ttggcggcta caagatgatt catgtcccac aacccatttg tggcccggaa gacgtagtga
     6961 ctgaaattaa agccgcggca atctgcggcg cagacatgaa gcactacaat gtcgatagcg
     7021 gttctgatga gtttaactct atccgcggcc atgagttcgc aggttgtatt gcgcaggttg
     7081 gtgaaaaagt caaagactgg aaagtggggc aacgcgtcgt gtcggataac agcggtcacg
     7141 tttgcggcgt ttgtccggcc tgtgaacagg gtgattttct gtgttgtaca gaaaaagtaa
     7201 accttggtct ggataacaat acctggggcg gtggtttttc caaatattgt ctggttcctg
     7261 gtgaaattct caaaattcat cgtcatgcgt tgtgggaaat ccctgatggt gttgattatg
     7321 aggacgcagc cgtacttgac cctatctgta atgcctataa atccatcgcg cagcaatcga
     7381 aattccttcc tggtcaggat gttgtcgtca ttggcactgg cccactcggg ctgttttccg
     7441 tacaaatggc gcggattatg ggggcggtaa atatcgtcgt cgttggtctg caagaagatg
     7501 tggcggtccg cttcccggtt gcaaaagaac tgggggcgac ggcagtagta aatggttcta
     7561 ccgaagatgt ggtggcgcgc tgccagcaaa tttgtggcaa agataatctg ggactggtga
     7621 ttgaatgctc cggtgccaat atcgcactga aacaagccat cgaaatgctc cgcccgaatg
     7681 gggaagtggt acgcgttgga atgggcttca aacctcttga tttctcgatt aatgacatta
     7741 ccgcctggaa taaaagcatc attgggcata tggcctatga ctccacctca tggcgtaacg
     7801 ctatccggct attagccagc ggcgctatca aagtcaaacc gatgatcacg catcgtatcg
     7861 gcctgtcgca atggcgcgaa gggtttgatg cgatggtcga taaaaccgca atcaaagtga
     7921 tcatgactta cgactttgat gaataactac tctcctgaag gaacgttatc gcatggaaca
     7981 gataacaaaa ccgcattgtg gtgcacggct ggatcgctta ccggattgcc gctggcattc
     8041 gtcaatgttt gccatcgtcg cgtttggctt gcttgtctgc tggagtaatg ccgttggtgg
     8101 cttgatcctc gcgcagctga aagcgttggg ctggacagat aattccacta cagccacatt
     8161 ctcagcaatc acgaccgccg gaatgtttct cggtgcgctg gtgggcggca tcattggcga
     8221 caaaaccggt cgcagaaatg cgttcatcct ctatgaggcc attcatattg cctcgatggt
     8281 tgtcggtgct ttctcaccga atatggattt tcttattgcc tgccgttttg tgatggacgt
     8341 tgggctggga gctttactgg ttacactgtt tgctggtttc accgaatata tgcccggtag
     8401 aaatcgcgga acgtggtcca gtcgggtttc ctttattggc aactggtcat atccgctctg
     8461 ctcactgatt gcgatgggac tcacgccgct gattagtgca gagtggaact ggcgggtaca
     8521 actgcttatt cctgcaatat tgtcgcttat cgctacggcg ctggcctggc gctactttcc
     8581 tgaatccccg cgctggctgg aatcgcgcgg acggtatcag gaagccgaga aagtcatgcg
     8641 gagtatagaa gaaggcgtca tacgccagac gggtaaacct ttaccgcctg tggttattgc
     8701 tgatgacggt aaagcgccac aagcggtgcc gtattcagcc ttactgacag gagtattact
     8761 gaaacgcgtg atattaggct cttgtgtgct gattaccatg aacgttgtgc agtacacact
     8821 aattaactgg ttgccaacaa tattcatgac ccaggggatt aatttaaaag actcgattgt
     8881 tttaaatacc atgagtatgt ttggcgcgcc atttggtatt tttattgcca tgctggtgat
     8941 ggataaaatt ccgcgtaaaa caatgggtgt ggggctatta atcctgattg cggtgctcgg
     9001 atatatctat tcactgcaaa ccagtatgtt gctcataacg ctgattggtt tcttcctgat
     9061 tactttcgtc tatatgtacg tttgctatgc ctcggcagtg tatgtccctg aaatctggcc
     9121 gacagaggcc aaactccgtg gctccggtct ggcgaatgcg gtagggcgaa tcagtggtat
     9181 tgccgcacct tatgccgttg cagtgctgct cagtagttat ggtgtaacgg gagtctttat
     9241 tcttctgggg gctgtttcaa atattgtcgc aattgctatc gccaccattg gaattgaaac
     9301 caaaggtgtc tccgttgaaa gtttaagtat tgatgcagta gccaataaat aatacgggtg
     9361 gattcaaaat gaaaaattca aaagcaatat tgcaggtgcc gggcacaatg aaaattattt
     9421 cagcagaaat accagtgcct aaagaagatg aagttttgat taaagtagaa tatgtcggta
     9481 tttgtggttc agatgtacat ggttttgaat caggcccgtt tattccgcct aaagacccaa
     9541 atcaagaaat tggcctgggt catgaatgcg ccgggacggt tgtggctgtg ggaagccgcg
     9601 tgcgcaaatt taaaccgggg gatcgggtaa atatcgaacc tggcgttcct tgcggtcact
     9661 gtcgttactg tctggaaggc aaatataaca tctgcccgga cgttgatttt atggcgacac
     9721 aacccaacta ccgcggcgca ttaacgcact atctgtgtca tccggagagc tttacttaca
     9781 aactgcccga caatatggac acgatggaag gggcgctggt ggagcctgcc gcagtcggga
     9841 tgcatgccgc gatgctggca gatgttaaac cgggtaagaa gataattatt ctgggagcag
     9901 gttgtattgg tttgatgacg ttgcaagcgt gcaaatgcct gggcgcaacg gaaattgccg
     9961 tcgttgatgt actggaaaaa cgtctggcaa taggggaaca gcctggtgcg acagtggtta
    10021 ttaatggcgc aaaagaggac actattgcac gctgtcagca atttaccgaa gacatgggcg
    10081 cagatattgt tttcgaaaca gcgggttctg cggtcaccgt taaacaggca cctttaatct
    10141 ggtaatgcgc ggcggtaaaa ttatgattgt tggtactgta cccggcgatt cggcaatcaa
    10201 tttcctcaaa atcaatcgcg aagtcactat ccagacggta ttccgctatg ccaatcgtta
    10261 tccggtcacg attgaagcta tttcttcagg gcgattcgat gtgaaatcga tggtgacgca
    10321 tatttacgat tatcgggatg tacaacaggc atttgaagag tcagttaaca acaaacgcga
    10381 cattattaaa ggcgttatta aaattagcga ttaattaata cgggagaaca gaacatgctc
    10441 gcagatatca gatattggga aaacgatgcc acaaataaat attatgcaat tgcccatttt
    10501 aacgtatgga atgcagaaat gttgatgggc gttaaagacg cagccgaaga agcgaaatcc
    10561 ccggttatta tttcttttag tacaggtttt gtcggtaaca cctcatttga agatttctct
    10621 cacatgatgg tatcaatggc acaaaaagca acggtgccgg taataactca ttgggatcat
    10681 ggtcggagta ttgagattat tcataacgcc tggactcatg gtatgaattc attaatgcgt
    10741 gatgcttctg catttgattt cgaagaaaat attcgtttaa ccaaagaggc tgttgatttc
    10801 tttcatccgc tgggtattcc ggtagaggcg gaattagggc atgtcggtaa tgaaaccgtt
    10861 tatgaggaag cgctggcggg ctatcactat accgatcctg accaggcggc tgaatttgtt
    10921 gaacgtacgg gctgtgactc attggctgtc gccatcggca accagcatgg ggtttatacg
    10981 tcagagccgc aattgaactt tgaggtcgtc aaacgcgtac gcgatgctgt ttctgttccg
    11041 ctggttttgc atggcgcatc ggggatcagt gatgccgaca ttaaaactgc aatttcgttg
    11101 ggtattgcga aaatcaacat tcatacggag ctctgtcagg ccgcaatggt agcagtgaaa
    11161 gaaaaccagg atcagccttt cctgcattta gaacgtgaag tacgtaaagc tgtaaaagaa
    11221 cgggcattgg aaaaaattga actgttcggt tctgatggca aagcggaata atgatatgga
    11281 taatctcgac gttatttgta taggtgccgc tattgttgat attccattac aaccggtcag
    11341 taaaaatatc tttgatgtag attcttatcc tcttgaaaga atcgcaatga gcaccggggg
    11401 cgatgcaata aatgaagcaa caattatttc tcgtctgggg catcgtaccg cattaatgag
    11461 ccgcattggt aaagatgcct cagggcaatt tattctcgac cattgtcgca aagagaatat
    11521 tgatattcaa agcctgaaac aggacgtcag catagataca tctattaacg ttggactggt
    11581 gacggaggat ggcgagcgga cgtttgtcac gaaccgtaac ggcagtttgt gggaattaaa
    11641 tattgacgat gttgattttg cgcggtttcc tcaggcgaaa ttattatcac tggccagtat
    11701 tttcaacagt cctctattgg atggtaaagc gctaacagaa atttttacgc aagcgaaagc
    11761 ccggcagatg atcatttgtg ccgatatgat caaaccgcgg ttgaatgaaa cgctggatga
    11821 tatttgcgaa gcattgagtt atgtcgatta tctgtttcca aattttgccg aggaaaaatt
    11881 actcacaggg aaagagacac tggatgaaat tgctgactgc tttcttgcgt gcggcgtaaa
    11941 aacggtggtg attaaaacgg gtaaagacgg ctgctttatc aagcgtggtg acatgacgat
    12001 gaaggtgccg gcggtcgcag gaataaccgc catcgacacc attggcgcgg gcgataactt
    12061 tgcttcaggt tttattgcgg cactgttaga aggcaaaaat ctgcgtgaat gcgcacgctt
    12121 tgccaatgca acggcggcta tctcggttct aagcgtcggt gccaccaccg gcgtaaaaaa
    12181 cagaaagctg gtcgaacaat tgctggaaga atacgaagga taatgaaggc aaatgaaaaa
    12241 gataccttta ggcacgacgg atataacgct ttcgcgaatg gggttgggga catgggccat
    12301 tggcggcggt cctgcatgga atggcgatct cgatcggcaa atatgtattg atacgattct
    12361 tgaagcccat cgttgtggca ttaatctgat tgatactgcg ctaggatata actttggcaa
    12421 tagtgaagtt atcgtcggtc aggcgttaaa aaaactgccc cgtgaacagg ttgtagtaga
    12481 aaccaaatgc ggcattgtct gggaacgaaa aggaagttta ttcaacaaag ttggcgatcg
    12541 gcagttgtat aaaaaccttt ccccggaatc tatccgcgaa gaggtagaag ccagcttgca
    12601 acgtctgggt attgattaca tcgatatcta tatgacgcac tggcagtcgg tgccgccatt
    12661 ttttacgccg atagctgaaa ctgtcgcagt gcttaatgag ttaaaggctg aagggaaaat
    12721 tcgcgctata ggcgctgcta acgtcgatgc tgaccatatc cgcgagtatc tgcaatatgg
    12781 tgaactggat attattcagg cgaaatacag tattctcgac cgggcaatgg aaaacgaact
    12841 gctgccgcta tgtcgtgata atggcattgt ggttcaggtt tattccccgc tagagcaggg
    12901 attgttgacc ggcactatca ctcgtgatta cgttccgggc ggcgctcggg caaataaagt
    12961 ctggttccag cgtgaaaaca tgctgaaagt gattgatatg cttgaacagt ggcagccact
    13021 ttgtgctcgt tatcagtgca caattcccac tctggcactg gcgtggatat taaaacagag
    13081 tgatttaatc tccattctta gtggggctac tgcaccggaa caggtacgcg aaaatgtcgc
    13141 ggcactgaat atcaacttat cggatgcaga cgcaacattg atgagggaaa tggcagaggc
    13201 cctggagcgt taaatactca acacgattca cctctgtttc tgtgaaaact gtgatgaaaa
    13261 tctgtgattc aaataggtta ttttgaaagt aaacatcggt tatgcgataa tcgcgctaat
    13321 gtgatgtaaa aagatacagg ggtgtaatcg tggcggcaaa agacaggatt caggcaatta
    13381 agcaaatggt tgccaacgat aaaaaggtga cagtctcaaa tttgagtggg atttttcagg
    13441 taaccgaaga aaccattcgc cgcgatcttg agaagctgga agatgaaggc tttttgacca
    13501 gaacctatgg tggtgctgtt ttaaatacag cggtgttgac ggagaatatc catttttata
    13561 agcgcgcttc atcgttttat gaagagaagc tgcttattgc acgcaaggca ctacccttta
    13621 tcgacaataa aaccaccatg gcagccgatt ccagtagtac cgtaatggaa ttgctcaaat
    13681 tgttacagga ccgtagtgac ctgacgttgc taaccaactc ggcggaagcg attcatgttc
    13741 tggctcagtc agaaattaaa gtcgtttcaa cgggtgggga actaaacaaa aatacacttt
    13801 ccctgcaagg aagaattact aaagagatca tcagccgcta ccatgtcgat atcatggtaa
    13861 tgagttgtaa aggtcttgat attaacagcg gcgcgctgga ctctaatgaa gcggaagctg
    13921 aaatcaaaaa gacaatgatc cgtcaggcga cagaagttgc gttattggtt gatcactcta
    13981 agtttgatcg taaagctttt gtccagttag ctgactttag tcatattaat tatataataa
    14041 ctgataaatc accgggtgca gagtggatag cattttgcaa agacaataat atccagctgg
    14101 tgtggtaatt taagcacacg ttgtcaatat tatttgttcc tgcgactgga aataaaatga
    14161 tgataaagat gcggcaagtg aatttgccgc aattgctacc ttttatagca aatccgaggc
    14221 acccaatgga acaatatgat caaattggcg caagactgga ccgcttgcct ttggcccggt
    14281 ttcattatcg tatatttggt ataataagct ttagtctgtt attaacgggg tttttgagtt
    14341 actcaggtaa tgtcgtctta gcaaagctgg taagcaatgg atggtcaaat aatttcctct
    14401 atgccgcctt tacctcggca ttaatgtttg gttatttcat cggctcactt actggtgggt
    14461 ttattggtga ctactttggg cggcgcaggg cgtttcgcat aaatcttctc atcgtcggta
    14521 ttgctgcaac aggggccgct tttgtccctg atatgtactg gctcattttc tttcgcttcc
    14581 tgatgggaac aggaatgggg gcgctgatta tggttggcta tgcctcattt acggagttta
    14641 tccccgcgac ggtgcgtgga aaatggtccg cgcggctctc atttgttggt aactggtcgc
    14701 ccatgctgtc tgcggcgata ggcgtggtgg ttatcgcttt ttttagttgg cgaataatgt
    14761 ttcttctggg tggtattggc atactgttag cctggtttct ctcaggtaaa tactttatcg
    14821 agtcgccacg atggctggaa gggaaagggc aaatcgccgg tgcagaaagc caacttcgtg
    14881 aagtagagca gcaaattgaa agggagaaga gtattcgttt accccagctt actttgaacc
    14941 agagcaacag caaggttaag gtaatcaagg gtaccttcag gctcctgttt aaaggggaaa
    15001 tgttacgacg tacattagtt gcgataactg ttttaattgc aatgaacatt tcgctttata
    15061 ccattaccgt atggataccg accatatttg ttaactccgg cattgatgtc gataaatcaa
    15121 tattaatgac cgctgttatt atgattggtg ctccggtagg aatatttatt gcggcattaa
    15181 ttattgatca ttttcctcgt cgattatttg gctccgcctt acttattatt attgccgtgt
    15241 taggctatat ctattcaagt cagactacag agtgggcgat tttaatctat ggtctggtga
    15301 tgatcttctt tttatacatg tatgtttgct tcgcgtcggc ggtttatatc ccggagcttt
    15361 ggccaacgca tttacgcctg cgcggttcgg gtttcgttaa tgccgtcgga cggatcgtcg
    15421 cagttttcac gccctatggc gttgcggcat tattaacaca ttatgggtcg atcacggtgt
    15481 ttatggtgct tggtgtcatg ttagtgctct gtgcgctggt tctctccatt tttggcatcg
    15541 aaacgcggaa ggtgtcgctg gaagagattt ctgaggtgaa ttaaatcttt cgttgatggc
    15601 atcaattgcc agatgtacca ctgacgtgtt ttatttggcc tacaatgcca gtacagaatt
    15661 gtaggccagc gtaaaattac ccctgcgtct cttcccagtc tgccagcgta tatagcgttg
    15721 ccccagctgc tgacatctcc ataaacgcgt gtgcactgtc ctggggctgg atattcacgc
    15781 cacgacagcc atcggtaatc acgtttacct tataacctaa ctgtaacgcg tccagcacgg
    15841 taaacttcac gcaatagtca gtagccaggc ccataacgat caattcatcg atttcatgat
    15901 cgcgcaacca gtcatcaagc gcggttttct gccgacgtcc gttatcaaaa aaggcactgt
    15961 aactgtcaac taaaggattt tcgcctttat ggaacaccgc tgcgatctct ttttgattca
    16021 gtaacggatg taattgtgcg ccttcactgt tctgcacaca gtgttcaggc cagaaagttt
    16081 gccgcaggcc gccgagttgg cctggcgtat aaggctctac gccgtgctgg ctggcaaagc
    16141 tgccgtgatt cgccggatgc cagtcctggc tggcgataac cgcttctccg cgcgactggc
    16201 accagtcaat aaggcggtta gcaacctcca ccgtactgtc accttccggt acggcgagcg
    16261 cgccaccagc acagaaatca ttttgtaaat cgaccagtaa cagggcgcga tggggcattg
    16321 cagtctcctt aatcatccgg cgtcagttcg ccgcgcaggt tttggctcat ggccttgcga
    16381 atggtttcag tatccagttc ctggctcagc aggtaatgca gtttggttag cgtggcttct
    16441 acagtcatat ctgcaccgtc aataacgccg gcatgggcga gggcgttacc ggtggcataa
    16501 ccactcatat tcactttacc ggacatacat tgtgtcaggt tgaccaccac aataccgcga
    16561 tcgctggctt cttgtaattc ctgcaggaag gctttgtttt gtggcgcatt acccacgcca
    16621 taggagcgca gaatcaatgc tttcaccggt tggcgcagaa aattgcgcac gacgtcagca
    16681 gaaatccctg gataaatcgt caccacgcca attggttgtg gcgtgattgg atgaacgatt
    16741 aattcccctt caccgtgcgg ggcgggtggc gtattcaaac gacgaatatg gataccagct
    16801 tccagtaacg gaggaaggtt tggagaggca aacgcatcaa aaccatcggc atgggctttg
    16861 gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga gcgttacttc gttgatcgga
    16921 taattcggcg caacgtacaa cgcattcagc agattaattt gtccgtcaga gcgtaactca
    16981 gccagcggga tttgtgaccc tgtcacaatg accggtttac cgagattctc gagcatgaac
    17041 gacagcgcag aggcggtata cgccatcgtg tcggtaccgt gtagaatgac aaaaccatca
    17101 tagtcgtcat agtgcgcttt aatatcttca gcaatatgct gccagtcttc cggcgtcata
    17161 tcagaagaat ccatcaacgg cgtatattca tgaatggtga aatctggcat ctccgggcga
    17221 tggaattccg gcatcagcgc cagttggcgt tgtagatgac ctgacaccgg aatataaccc
    17281 tgctcggaac gctgcatccc gatggtcccg cccgtgtagg cgacgtaaat tgatttcttt
    17341 tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt atatacgtga ggcaggagga
    17401 gcgcacctga tactatgcgg ataaggaaat atggggcgag acagcgggaa gaggattcgt
    17461 cgaggtaaaa aataatgtgg ccgacgatct gtcggccact cagtacaaga cttaacgcac
    17521 gttggcgcag gtcaggcaaa acgcataacg gttttgtggg tcgttaaacg cggccagctt
    17581 gtcgctttca cttttaacgg tagaggctac cgaggcaagc ggtgcaggta acatggcctg
    17641 gaacgcatct ggcaacattg cccggacaga accagacatg ttgtccatca ctttgtcgaa
    17701 gaaggtcggt tcatcaacgt agtattccag atgccactgt ttcacttttg cgagctctgc
    17761 tgctttggca accgcatcat cgaaatcccc gagactatcg accagcccgt tagcttttgc
    17821 atcctgaccg gtccagacgt ggccctgggc gattttatca atttgctccg gcgtcgaatg
    17881 acgcgcatca gcaaccagcg tgataaagcg tttatagcca ttctcaatgc ttaattgcat
    17941 catctgctgc gcttccggcg gcagtgccct ggtgatagaa acatccgcca gcggtgaagt
    18001 tgagacacca tcagtatgaa caccaatcga atccagacta ttttctacgg tggtgatcac
    18061 gccgaagata ccgatagaac cggtcagggt gctggggtta gccacaatgt aattagctgg
    18121 cgtggaaatc cagtaaccac cagatgccgc catgccgccc atcgatacaa ccacagactt
    18181 acccgctgcc cgggctgctg ccagttcagc gcgaatcact tcagacgcgg taacgctgcc
    18241 gcctgggcta ttaacacgca ggacaatcgc tttcactttc gggtcaaggc gagcgtcgcg
    18301 gatttgtgcc gcagtggtat caccgccaac attcccctga gtttcctcgc catccataat
    18361 tgcgccatta gcgaagacga caccgatgct gtcaccggta tctgccggcg ttttcaatgc
    18421 gtaatcgtaa taactgatgg cgcgataatt tttatcagtc ttactccagc cgaactcttt
    18481 ggtcagagct ttttcgattt cggcactcga tgccagtgca tcgaccagct tgttttccag
    18541 tgcatatttc gcggtatcgc caccggtttt ggttaaaccc tcaagcaacc cttgcgcgcc
    18601 agggaatacc tgctgagcag ggatctgccg gttagcggca acagtattca gatagttttg
    18661 ccacagctca ccaatccagc ggctgtcagc ttcgcgggct gccggtgaca tatcatcacg
    18721 aataaacggt tcaacggcag atttatacgt acccacgcgg aacacatggg tggaaacttt
    18781 cagcttatcc agcaacgatt tgtagtacag accgttggta gcaaaaccgt gcagatcaac
    18841 cacgccttgc ggtgacagcc aaattttatt ggcgaaactg gcgagataat attgcccctg
    18901 gctgtagttc tcgccaacgg cataaaccgg tttcccgctg tcacgaaact ctttcagggc
    18961 tttgccgatg tactgcatag acggctggtc gccgcctgcg aagtttttca gatccatcac
    19021 gataccggtg atattgcggt cgtccttcgc ctggcgaatg gtgttgacga tatcaaacag
    19081 tgagttttcc tgcagacgat cggaactggc accaagcagc tggcggctta atttactaaa
    19141 ccgctgagaa ctgtctggtt tatcgacgat cacaccagaa atgtccagca gcagtgcgcc
    19201 acgactggcc gtttctttcg aatcaccacc actgacctgc atccagatcc ccacaccaac
    19261 cagtacgagg aaaataaaga acaggttaag taccatttca cggacgaaat tcagcagacg
    19321 ccacgtccat ttaaaaaatc cggcaataaa tcgccaaagg gttcgcatgt attctcccaa
    19381 cttaaggtca cacctgtcac aggcgcaata gatgtggtta tcgtaatgac ccaaccggca
    19441 cttgtcagca ggaattgcgg actgtgctgt aacaaaatcc actctcgtgt taattttgtg
    19501 aataaatatc acgacaggag ttaatcaaat ggatgcactc gaactattga tcaatcgccg
    19561 tagcgcctcc cgcctggctg aacccgcgcc aacgggtgaa caactgcaaa acatcctgcg
    19621 tgcgggtatg cgtgcgccgg accataagtc catgcaaccg tggcattttt ttgtgattga
    19681 aggggaaggg tgcgagcgtt tcagcgccgt actggaacag ggggcgattg ctgccggtag
    19741 tgatgataaa gctatcgaca aagcccgtaa tgcgccgttc cgcgcaccgc tcatcatcac
    19801 ggtggtggcg aaatgcaaag aaaatcataa agtcccgcga tgggaacagg aaatgtctgc
    19861 cggatgcgcg gtcatggcga tgcaaatggc agccgttgcc cagggatttg gcggcatctg
    19921 gcgcagtggc gcattaactg aaagtccggt agtgcgtgaa gcattcggtt gccgtgagca
    19981 ggataaaatt gtcggttttc tctacctcgg cacgccgcag ttgaaagcat ctacgtcgat
    20041 taacgtcccg gacccgacgc cgtttgtaac ttatttctga taattgtcgc gaaactgtct
    20101 ggattgtgag caaaggcgca ggaattcaga cactctcact tatcacttca cggaatgagg
    20161 gttaccatac ccggattgca ttgacaggag atgtccatga gcgagaactc gattcgtttg
    20221 acccaataca gccacggagc tggttgcggc tgtaaaattt ccccaaaagt gttggaaacc
    20281 atcctgcaca gtgagcaggc gaagtttgtt gatccgaatt tgcttgtggg taatgaaacc
    20341 cgcgacgatg cggcggtgta cgatctgggc aatggcacca gcgttatcag taccaccgac
    20401 ttctttatgc cgatcgttga taatcctttc gattttggcc gcattgcggc gactaacgcc
    20461 atcagcgata tcttcgcgat ggggggcaaa ccgattatgg cgattgcgat cctcggctgg
    20521 ccgattaaca aactttcccc ggaaattgcc cgcgaagtaa ccgaaggtgg acgctatgca
    20581 tgccgtcagg cgggtattgc gctggctggc ggtcactcca tcgatgcgcc ggagccgatt
    20641 tttggtctgg cggtaacggg gatcgtaccg accgagcggg tgaagaaaaa cagtaccgca
    20701 caagccggat gcaaactgtt cctgacgaaa ccgctgggga tcggcgttct taccacggct
    20761 gagaaaaaat cactgttgaa accagaacat cagggactgg cgacggaagt gatgtgccgg
    20821 atgaacatcg caggcgcgtc ctttgccaac atcgaaggcg taaaagcgat gaccgacgtt
    20881 acgggctttg gtctgctggg ccacttgagc gaaatgtgtc agggggctgg tgtgcaggca
    20941 cgcgtcgact atgaagcgat cccgaaactc cccggtgttg aagagtacat taagttgggc
    21001 gcagtacctg gcggcactga acgtaacttt gccagctacg gtcatctgat gggtgaaatg
    21061 ccgcgtgaag tgcgcgatct gctgtgcgat ccgcaaactt ctggcggttt gctgctggcg
    21121 gtcatgccgg aagcagaaaa tgaggttaaa actacagccg ccgagtttgg cattgaactg
    21181 acggcgattg gcgaactggt gccagcgcgc ggcggtcgtg ccatggttga gatccgttaa
    21241 tttaatgcgg ttgtttattg ccgaaaaacc gagtctggcg cgcgccattg ctgatgtcct
    21301 gcccaaaccg caccggaaag gcgatggctt tatcgagtgc ggtaatggtc aggtggtgac
    21361 ttggtgtatc ggtcacctgc ttgagcaggc gcagccagac gcctacgaca gccgctatgc
    21421 gcgctggaat cttgcggatt tgccgattgt cccggaaaag tggcaattac agccacgacc
    21481 ctccgtgacc aaacaactta acgtcatcaa acgcttcctg catgaagcca gcgaaatcgt
    21541 tcacgccggg gacccggatc gtgaagggca attgctggtg gatgaagtgc tggactatct
    21601 gcaactggca ccggaaaagc gccagcaggt gcagcgttgc ttgataaacg acctgaaccc
    21661 gcaggcggtt gagcgggcga tcgaccgtct tcgttccaac agtgagtttg tgccattgtg
    21721 cgtttctgcg ctggcgcgag cgcgtgccga ctggctgtac ggcatcaata tgacccgtgc
    21781 gtataccatt ctcggtcgca atgccggtta tcagggcgta ctttccgtgg gacgcgtgca
    21841 gacacccgtg ctcgggctgg tggtgcgccg cgatgaagag attgaaaact tcgtggcgaa
    21901 agacttcttt gaagtcaaag cacatatcgt gacacctgcc gatgagcggt ttaccgctat
    21961 ctggcaaccg agcgaagcgt gtgaaccgta ccaggatgaa gaagggcgct tgttacatcg
    22021 tccactggcg gagcatgtgg ttaaccgcat tagtggtcaa ccggctattg tcaccagcta
    22081 taacgataaa cgggaatcag aatccgcgcc gctgccgttt tcgctttcgg cgttgcagat
    22141 tgaagcggca aaacgcttcg gtctgagcgc gcagaacgtg cttgatatct gccagaagct
    22201 gtacgaaacg cacaagctaa tcacttatcc gcgttccgat tgtcgctatt tgccagaaga
    22261 acattttgcc ggacgccacg cggtgatgaa tgccatcagc gttcatgcac cggatctgtt
    22321 gccgcagcca gtggtagatc cagatatacg caaccgctgt tgggatgaca aaaaggtcga
    22381 tgcgcaccac gccatcatcc cgaccgcacg gagttctgcg atcaacctga cggagaacga
    22441 agcgaaggtc tataacctga ttgcccgtca atacctgatg cagttctgcc cggatgcggt
    22501 gttccgcaag tgtgttatcg aactggacat tgccaaaggc aaatttgtcg ctaaagcccg
    22561 ttttcttgct gaagcaggct ggcgcacgct gttaggcagc aaagagcgcg atgaagaaaa
    22621 cgacggcacg ccattgcctg tggtggcgaa aggcgatgag ttgctgtgtg aaaaaggtga
    22681 agtggtagag cggcaaaccc agccgccgcg ccattttacc gatgcaacac tgctttcggc
    22741 gatgaccggg atcgcgcgct ttgtgcagga caaagatctg aaaaaggtcc ttcgtgcgac
    22801 cgatggtctg gggacagaag caacccgtgc cgggattatt gaactgctgt tcaagcgtgg
    22861 ttttctgacc aaaaaagggc gctatatcca ctccaccgac gccggaaaag cgttattcca
    22921 ttcgctgcca gaaatggcga cgcgaccgga catgaccgcg cactgggaat cggtgctgac
    22981 gcaaatcagc gaaaagcagt gtcgctatca ggactttatg cagccgctgg tggggacgct
    23041 atatcagctt attgatcaag ccaaacgtac gccggtgcgg cagtttcgcg gcattgtggc
    23101 tccgggcagt ggtggcagtg ctgataagaa aaaggctgca ccgcgtaaac gtagtgcgaa
    23161 aaaaagtccg ccagcagatg aagccggaag cggggcgata gcgtaagcga gcgaatcttt
    23221 cgtgctattc gagtcatatt ctgaaatatc cagcggatca agaaagttcg ttggatattt
    23281 ttttgcatgg ataaaattat cgcctctaaa gtatgtaata acagggaatg tgatgaaaaa
    23341 agttttattg caaaaccatc ctgggagcga gaagtattct tttaatggct ggggaatatt
    23401 taatagtaat tttgaacggg tgattaaaga aaataaggcc atgctgcttt gtaagtgggg
    23461 gttttattta acatgtgttg tcgctgtaat gtttgtattc gcagcgataa catccaacgg
    23521 tttgaatgaa agaggcctga ttaccgcggg atgctctttt ctttatctat taattatgat
    23581 ggggcttatt gttcgggccg gttttaaagc aaaaaaagaa caactgcatt attatcaggc
    23641 taaaggtatt gagccgctca gtatcgagaa gttacaggcg ctacaattga tcgcacctta
    23701 tcgattctat cataagcaat ggtctgaaac gctagagttc tggccgcgaa agcctgaacc
    23761 tggcaaagat accttccaat atcatgtgct tccttttgac tcgatcgata tcatcagtaa
    23821 aagacgcgag tctttagaga atcaatgggg tatcgaagat agcgaaagtt attgtgcctt
    23881 aatggagcat tttctttctg gcgaccatgg agccaatacc tttaaagcaa acatggagga
    23941 agccccagag caggttatcg ccttgttgaa taaatttgct gtttttccct cagactatat
    24001 ctctgattgc gctaataatc gctccggtaa atcctcggcg aagctaatat gggcggcgga
    24061 attatcatgg atgatctcga tatcaagcac agcttttcaa aacgggacaa ttgaagaaga
    24121 actggcctgg cattatataa tgcttgcttc tcgaaaggcg cacgagttgt tcgaaagcga
    24181 agaagattat caaaaaaata gtctaatggg atttctttac tggcatatct gctgctatcg
    24241 cagaaagtta acggatgctg aacttgaagc gtgttatcgt tacgacaagc agttttggga
    24301 gcactacagt aagaaatgcc gttggcccat aagaaatgtt ccctggggag catcatccgt
    24361 taaatactca taaacgcctg aaattttgcc gggggcgctg cgcttgaccg gcctacatta
    24421 gagcgtaact gcaatgaatt gtgcccatct gtaggcctga taagcgtagc gccatcaggc
    24481 atttacaact taaatcacac cctgcgccag catcgcatcg gcaaccttca caaaaccggc
    24541 aatgttcgcg ccctgcacgt agttggtttg ctcaccttca ccaccatgct caacacaggc
    24601 atggtggata tccagcatga tgtgatgcaa acgtgcgtca actttctcgg ctttccagcc
    24661 caggcgcgca gcgttttgtg ccatttccag gcccgacgtt gctacaccgc cagcattagc
    24721 cgctttaccc ggtgcaaaca gtacgcctgc ctgctggaac agttcagtcg cttcgatggt
    24781 ggtcggcata tttgcccctt cagcgacggc tttaacgcca ttggcgataa gctgatgcgc
    24841 ggcgtcaaca tccagttcgt tctgggtggc gcaaggcagg gcgatatcaa ccggtacaga
    24901 ccacggctgt tggccttcga gatagaccag accaaattct ttggcgtaat ctgccgctcg
    24961 accatcgcgg ctggctttga tttcgataag acgtgccagt ttctctttcg tgaatccgct
    25021 ttcatcaact acagtgccgc tggagtctga cgcagtgatc acacgagcac caaattccat
    25081 cgctttttcg atagcgtact gggcgacgtt gccggagcca gaaacggaaa cgctcatccc
    25141 ttcaaaaccc ataccgtggc gttttaacat tgcttctgtg aaataaacca gaccgtagcc
    25201 ggtagcttcc gggcgaataa gactaccgc
//
LOCUS       DAJTPG010000056        24643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTPG010000056 DAJTPG010000000
VERSION     DAJTPG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(1..386)
                     /locus_tag="OO230_002352"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO230_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO230_002353"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO230_002353"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5692078.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO230_002354"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO230_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5692079.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO230_002355"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO230_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5692080.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO230_002356"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO230_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5692081.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO230_002357"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO230_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5692082.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO230_002358"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO230_002358"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5692083.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO230_002359"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO230_002359"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5692084.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO230_002360"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO230_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5692085.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO230_002361"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO230_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5692086.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO230_002362"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO230_002362"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5692087.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO230_002363"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO230_002363"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5692088.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO230_002364"
     CDS             10748..11212
                     /locus_tag="OO230_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5692089.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO230_002365"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO230_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5692090.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO230_002366"
     CDS             12236..13672
                     /locus_tag="OO230_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5692091.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO230_002367"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO230_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5692092.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO230_002368"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO230_002368"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5692093.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO230_002369"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO230_002369"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5692094.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO230_002370"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO230_002370"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5692095.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO230_002371"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO230_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5692096.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO230_002372"
     CDS             complement(20505..20798)
                     /locus_tag="OO230_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5692097.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO230_002373"
     CDS             complement(20795..22084)
                     /locus_tag="OO230_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5692098.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO230_002374"
     CDS             complement(22140..23078)
                     /locus_tag="OO230_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5692099.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO230_002375"
     CDS             complement(23098..23862)
                     /locus_tag="OO230_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5692100.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24181..>24643
                     /locus_tag="OO230_002376"
     CDS             24181..>24643
                     /locus_tag="OO230_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5692101.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALA"
BASE COUNT         6099 a         5947 c         6483 g         6114 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgtctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gacaatgaaa ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    24361 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    24421 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    24481 tttcaggggg ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg
    24541 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    24601 agtaaaaata gccaaacatc ttcgttatgg gatgcattag cct
//
LOCUS       DAJTPG010000057        24172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTPG010000057 DAJTPG010000000
VERSION     DAJTPG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(22..594)
                     /locus_tag="OO230_002377"
     CDS             complement(22..594)
                     /locus_tag="OO230_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5692102.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO230_002378"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO230_002378"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5692103.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO230_002379"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO230_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5692104.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO230_002380"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO230_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5692105.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO230_002381"
     CDS             complement(3556..5337)
                     /locus_tag="OO230_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5692106.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO230_002382"
     CDS             complement(5330..7102)
                     /locus_tag="OO230_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5692107.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO230_002383"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO230_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5692108.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO230_002384"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO230_002384"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5692109.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="OO230_002385"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="OO230_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="OO230_002386"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="OO230_002386"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5692110.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO230_002387"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO230_002387"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5692111.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="OO230_002388"
     CDS             complement(11658..12029)
                     /locus_tag="OO230_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5692112.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO230_002389"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO230_002389"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5692113.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO230_002390"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO230_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5692114.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO230_002391"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO230_002391"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5692115.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO230_002392"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO230_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5692116.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO230_002393"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO230_002393"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5692117.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO230_002394"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO230_002394"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5692118.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO230_002395"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO230_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5692119.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="OO230_002396"
     CDS             21800..22378
                     /locus_tag="OO230_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5692120.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="OO230_002397"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="OO230_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5692121.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5665 a         6663 c         5826 g         6018 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cc
//
LOCUS       DAJTPG010000058        23866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTPG010000058 DAJTPG010000000
VERSION     DAJTPG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(88..960)
                     /gene="rluF"
                     /locus_tag="OO230_002398"
     CDS             complement(88..960)
                     /gene="rluF"
                     /locus_tag="OO230_002398"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5692122.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1308..2255
                     /locus_tag="OO230_002399"
     CDS             1308..2255
                     /locus_tag="OO230_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5692123.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2325..3131
                     /locus_tag="OO230_002400"
     CDS             2325..3131
                     /locus_tag="OO230_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5692124.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3141..3548
                     /locus_tag="OO230_002401"
     CDS             3141..3548
                     /locus_tag="OO230_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5692125.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3548..4042
                     /locus_tag="OO230_002402"
     CDS             3548..4042
                     /locus_tag="OO230_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5692126.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4108..4905
                     /locus_tag="OO230_002403"
     CDS             4108..4905
                     /locus_tag="OO230_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5692127.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4916..5704
                     /locus_tag="OO230_002404"
     CDS             4916..5704
                     /locus_tag="OO230_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5692128.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5756..6985
                     /gene="sorE"
                     /locus_tag="OO230_002405"
     CDS             5756..6985
                     /gene="sorE"
                     /locus_tag="OO230_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5692129.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7367..8056
                     /gene="pepE"
                     /locus_tag="OO230_002406"
     CDS             7367..8056
                     /gene="pepE"
                     /locus_tag="OO230_002406"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5692130.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8147..9778)
                     /locus_tag="OO230_002407"
     CDS             complement(8147..9778)
                     /locus_tag="OO230_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5692131.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9998..13681)
                     /gene="metH"
                     /locus_tag="OO230_002408"
     CDS             complement(9998..13681)
                     /gene="metH"
                     /locus_tag="OO230_002408"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5692132.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13881..14705
                     /gene="iclR"
                     /locus_tag="OO230_002409"
     CDS             13881..14705
                     /gene="iclR"
                     /locus_tag="OO230_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5692133.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15022..17206
                     /gene="espL4"
                     /locus_tag="OO230_002410"
                     /pseudo
     CDS             15022..17206
                     /gene="espL4"
                     /locus_tag="OO230_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17175..18912)
                     /gene="aceK"
                     /locus_tag="OO230_002411"
                     /pseudo
     CDS             complement(17175..18912)
                     /gene="aceK"
                     /locus_tag="OO230_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19096..20400)
                     /gene="aceA"
                     /locus_tag="OO230_002412"
     CDS             complement(19096..20400)
                     /gene="aceA"
                     /locus_tag="OO230_002412"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5692134.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20430..22031)
                     /gene="aceB"
                     /locus_tag="OO230_002413"
                     /pseudo
     CDS             complement(20430..22031)
                     /gene="aceB"
                     /locus_tag="OO230_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22300..23229)
                     /gene="metA"
                     /locus_tag="OO230_002414"
     CDS             complement(22300..23229)
                     /gene="metA"
                     /locus_tag="OO230_002414"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5692135.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23386..23829
                     /locus_tag="OO230_002415"
     CDS             23386..23829
                     /locus_tag="OO230_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5692136.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5868 a         6095 c         6054 g         5849 t
ORIGIN      
        1 tcttcccctc accctaaccc tctccccgaa ggggcgaggg gactgtccga gctctttttg
       61 gacgttttca tcagtctgag gcgttaatca gcgtcccttc tttttacgcc ccggcgaggt
      121 aaaacgctta ccgttggacg ccgggcgacc gcctttttcc gccgtttttt ccatcttaac
      181 gactggacgt ttgatgcccg ctgttttcgg tttggccttc gctttaggtt ttgcctcgga
      241 agaggaattt tcgatgagct taaagaggtc gattaactca tcgtcggtta aatcgcgcca
      301 ctcccccagc ggaatgccgc ttaagctgac gttcataatg cgcgtacgtt ccagcttttt
      361 cacttcatag ccgaaatgct cgcacatgcg ccggatctga cggttcagcc cctgcactag
      421 ggtaatgcgg aagacaaacg gcgcttcttt tttgactttg cactttttcg tcaccgtccc
      481 gaggatcggc acccccgccc ccataccacg aataaattca tcggtaatcg gtttatcgac
      541 cgtcaccaga tactctttct catgatcgtt acccgcacgg aggatcttat tcaccagatc
      601 gccgtgattg gtgaggaaaa tcagcccctg ggagtcttta tccaggcggc caatcgggaa
      661 cacgcgtttg ctgtggttaa cgaaatcgac aatgttatcg cgctcgccat cttcggtggt
      721 gctgacaata ccgacgggct tgttcagggc gataagtacc aaatcttcgg cttcccgagg
      781 ctcaatcaac tgaccgttta ctttcacaat gtcgccgggt ttcacctgat cgccaatggt
      841 ggctcgcttg ccattaagga acacattgcc ttgctcgata tagcgatccg cttcgcggcg
      901 tgagcaaatt ccgctttcgc tgatgtattt atttaaacgg actgatgagt cgggcagcat
      961 aaattctcct gtaaaacgcg gaatataccg tacctcttag cggggaaaaa cagggttatg
     1021 cggcaacgtc gaaattaaat gtgatctgcc acgcctttta tcacaaaagt gtctccggaa
     1081 gtacgatgca aatttgcctg gcaaaataag gataatcaac atattacgaa tcgctgcaca
     1141 cctgtgctga tgactactac gcagagaaac aaatgtgcaa gagaaagcgc tatttctccc
     1201 catgcaaaga gtgaaaaatc gctttgaaag gccctcatga tggggttata tcagatcaag
     1261 cggtgagaaa gcgtatttgc acaaatgtgc agaaccagga ggcggcaatg gaaaacagtg
     1321 acgatatccg tttgattgtg aagattgccc aactctatta cgaacaggat atgacgcagg
     1381 cgcaaatcgc gcgcgaactg ggtatttacc gcaccaccat cagccgcttg cttaaacgag
     1441 gccgcgatca gggaatggtc accatcgcca tcaactatga ctacaacgaa aatctctggc
     1501 tggagcagca actgaagcaa aagtttggcc tgaaagacgt tgtggtggtg tcgggaaatg
     1561 atgaggatga agagactcaa ctggcgatga tggggttaca cggcgcgcaa ctgctggatc
     1621 gcttgctgga gcctggcgat attgtcggtt tttcctgggg tcgcgcggtg agcgcactgg
     1681 ttgaaaactt gccgcaggcg gggcaatcgc ggcagttaat ctgcgtacca attattggcg
     1741 gaccgtccgg taaactcgaa agccgctatc atgtaaacac attaacctac agcgcggcag
     1801 cgaagctgaa aggggaatcg catctcgcgg attttccggc tctgctggat aacccattaa
     1861 ttcgtaatgg gatcatgcag tctcagcact ttaaaaccat ctctgcctac tgggataatc
     1921 tggatatcgc cctggtggga attggctcac cggccattcg cgacggcgct aactggcatg
     1981 cgttttatgg tggtgaagag agtgacgacc tgaatgcccg ccaggttgct ggcgatattt
     2041 gctcgcgctt ttttgatatt cacggcgcaa tggttgaaac gaatatgagc gaaaaaacgc
     2101 tctctatcga aatgaataaa ttaaagcagg cgcgatattc cattggcatt gccatgagtg
     2161 aagaaaaata cagcggaatt attggtgcac tgcgtggaaa atatattaat tgtctggtaa
     2221 cgaatagcag cacagctgaa ctattactga aataaaaatc ttcccgatgt ttaccgtaat
     2281 aaaaattacg gcgggtccct attatctaaa atcaggagtc tgttatgcaa acgtggttaa
     2341 atttgcagga taaaatcatt attgtcaccg gcggcgcatc cggtattggt ctggcgattg
     2401 tagaggaatt attagcacaa ggcgcgaatg tacagatggt cgatattcac ggtggcgatg
     2461 gtcaatatga aggtcataaa ggctatcagt tttggccgac cgatatttct agcacaaaag
     2521 aggtaaatca tacggtagcg gaaattatcc agcgttttgg tcgcatcgac ggtctggtca
     2581 ataacgccgg ggtcaatttc ccgcgtctgc tggtcgatga gaaagcgcct gccgggcagt
     2641 atgaactcaa cgaagctgca ttcgaaaaaa tggtcaatat caaccagaaa ggtgtttttc
     2701 tgatgtcgca ggcggtggcg cgacagatgg tcaaacaaca tgatggcgtg attgtgaatg
     2761 tttcctcaga aagtgggctg gaaggctcag aaggacaaag ctgttacgcc gctaccaaag
     2821 ccgcgctcaa tagcttcacg cgctcctggt cgaaagagct gggtaaacac ggtatccgtg
     2881 tggtcggtat cgcgcccggg attctggaaa aaaccgggct acgcacgccg gaatatgaag
     2941 aggcgctggc gtggacgcgc aatatcaccg tcgagcagct gcgtgaaggc tataccaaaa
     3001 acgccattcc tattgggcgc gccggaagat tagcagaagt agctgatttt gtttgttatc
     3061 tgttgtctga acgcgccagc tacatcaccg gagtaaccac taacattgcg ggcggcaaaa
     3121 cgcgcggcta aggaggcttt atggtcaacg ccattttttg tgcccacggc aaattagctt
     3181 gcgccatgct ggaatcggtt cagatggttt acggcaacgc caatgttgaa gccgttgcat
     3241 ttgtgcccgg tgaaaacgcc ggagacattg cggtaaagct ggaaaagtta gtaagcattc
     3301 acaatcaaga tgagtggctg attgccgtgg atttacagtg cggcagcccg tggaatgcgg
     3361 cagcaatgct tgcgatgcgc aatcctcgtt tgcgggtaat cagcggcctt tctctgccgc
     3421 tggcgctcga actggttgat aaccaggaca gcatgaatgt tgatgaactg tgtgaacacc
     3481 tgacgcaaat cgcaaagcaa acctgcgttg tctggaaaca actggcaacg acagaggagg
     3541 atttctgatg aacattacgc tcgcccgcat tgatgaccgt ctgatccacg gccaggtcac
     3601 taccgtctgg tcgaaagtgg ctaacgccca gcgcattatt atctgcaatg acgaagttta
     3661 taacgatgaa gtgcgccgga cattattgcg tcaggctgcg ccaccgggaa tgaaggttaa
     3721 cgtggtcaat attgaaaaag ccgtcgcggt ttatcacaac ccgcaatatc aggacgaaac
     3781 cgttttttat cttttcactc gcccacagga tgccttagcg atggttcgcc agggggtaaa
     3841 aataggcacc ctgaatattg gcggcatggc ctggcggccc ggtaaaaaac agttaaccaa
     3901 agcggtttca ttagatgatg atgatattaa cgcatttcat gagttaaata atcttggtgt
     3961 catactggat ttacgcgtcg tagcgtcaga tccctcaatc aatattatcg acaaaataaa
     4021 cgaacagtta atcgcaaatt aaataatagc gcctgtgtac atatgcttta acaggcaggg
     4081 atgacgacac ttaaaaggtg acaaatcatg gaaataagta ccctacaaat cattgctata
     4141 tttctttttt cctgtattgc cggaatgggc agcgtgctgg atgaatttca gactcatcgc
     4201 ccgttaattg cctgtacggt gattggttta attctcggtg atttaaaaac cggaataata
     4261 ctcggcggta cgctggaatt gatagctctc tgctggatga acgtcggcgc ggcgcaatct
     4321 ccggattctg cactcgccag cataatctcc gccattctgg ttatcgttgg tcagcaaagc
     4381 atcgccaccg gaatcgccat cgcgttgcct gtggctgcgg caggccaggt gctgacggtg
     4441 tttgcccgta ccatcaccgt agtgttccag cacgcggcgg ataaagcagc agaagaggcg
     4501 cggtttcgca ctctcgatat tctgcatgtc tccgcgcttg gcgtgcaggc gctgcgcgtc
     4561 gccattccgg cactgattgt ctcactgttc gtcagcgccg atatggtcag caatatgtta
     4621 aacgccattc ccgaatttgt gacccgagga ctacagattg ctggcggttt tatcgtggtg
     4681 gtcggttacg ccatggtgct acgtatgatg ggcgtgaaat atttgatgcc tttctttttc
     4741 ctcggtttcc tcgcaggtgg ctacctcgat ctcagcctgc tggcgttcgg tggcgtcggc
     4801 gtgatcatgg ccctggtcta catccagcta aatccacagt ggtgtaaagc tgaacctcaa
     4861 ctccaggcca ccgcctccac cgcccttgac caacttgacg attaacggag ccatcatgga
     4921 acagagaaaa attacacgca gcgatctggt gagcatgttt ctgcgctcca acctgcaaca
     4981 ggcgtccttt aactttgaac gtattcacgg gctgggcttt tgctacgaca tgatccccgc
     5041 catcaagcga ctttacccat taaaagagga tcaggttgcg gcgctcaggc tacacctggt
     5101 gttcttcaat accacgccag ccgtatgtgg cccggtcatc ggcgcggaaa ttgatgacgg
     5161 taccatcaac ggcatcaaag tcggtctgat gggaccattg gcaggagttg gcgatccact
     5221 ggtctgggga acgctgcgcc cgattaccgc cgcgctcggc gcatctctgg cactttcggg
     5281 caacattctc ggcccgctgc tgttcttctt tattttcaac gcggtgcgtc tggcgatgaa
     5341 gtggtatggc ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg
     5401 gaatgtgctg caaaaactca ccgaaggcgc gtcgattctc ggactgtttg tgatgggcgt
     5461 gctggtgacc aaatggacgt caatcaacgt accgttggtg gtttcacaaa cgcatgccgc
     5521 cgatggctcc accgtcacca tgaccgtgca gaacattctc gaccaacttt gccctggttt
     5581 gctggcgctc ggtctgacgc tactaatggt tcgtctgctc aacaaaaaaa ttaacccggt
     5641 atggctgatt ttcgccctgt ttggcttagg gattatcggc aatgctctgg gcttcctgtc
     5701 ctgattcttc gccccggcac gactgccgag gccatcgctc aacatgaggt ggtttatgaa
     5761 aacaacagct ctgcgtcttt atggtaaacg tgatttacgc ctggaaacct ttgaccttcc
     5821 tgaaatgcag gaggatgaaa tcctcgcaac ggtggtcact gacagcctgt gcctctcttc
     5881 ctggaaagag gccaatctgg gtgaaaacca taaaaaagta cccgacgatg tgacgaccaa
     5941 cccaatcatc atcggccacg aattttgcgg cgatattctg gccgtgggta aaaagtggca
     6001 gcacaaattc cagccgggtc agcgttatgt gattcaggcc aacctgcaac tccccgaccg
     6061 cccggattgc cccggctact ccttcccgtg ggtagacggc gaagcgacgc atgtggttat
     6121 tcccaacgag gtcatggaac aatattgcct gctggcatac gacggcgaaa cctattttga
     6181 aggctcactg gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca actatcatct
     6241 tcaggaaggt agttataacc acacgatggg gattcgcccg caagggcaca cgctgatcct
     6301 cggcggcacc ggaccaatgg gactgttggc gattgattat gcgctgcatg gaccagttaa
     6361 cccgtcgctg ctcgtcatta ccgataccga caacgacaaa ttgagttatg cgcgcaagca
     6421 ctatccatca gaaccgcaaa cactgattca ttatctcaat gccaccgatg cagcatttga
     6481 tacgttaatg gcgctgagtg gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa
     6541 tgaaggactg gtgactctcg cctcttcctt gctggcgaca gatggttgcc tgaatttctt
     6601 cgccggaccg caggataaac atttcagcgc gccaattaat ttctacgatg tgcattatgc
     6661 atttacccac tacgtgggca cgtcaggcgg caataccgac gacatgcgcg cagcggtcaa
     6721 actgatcgaa gagaaaaaag tgcaggccgc aaaagtggta acacatattc ttgggctgaa
     6781 tgccgcgggc gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta
     6841 taccgggaaa tacctgccgc tgacgtcact cacgcagatt caggatcaag cactggcggc
     6901 gattctggcg cgtcatcagg ggatctggtc cggtgaggtg gagcaatacc tgctcgccca
     6961 tgcagaggca atttcccatg attaatcgcg ctacttaact ctgcatgtcg ctggctggtc
     7021 gccccagcaa cttcgcgacc ggatgtgcgg ctgattgctg aagcagcaca atttgcacga
     7081 gaaggataaa aaatatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg
     7141 tttatgccgg atgcggaatg aagccttatt cggtctacat aattttgaaa atccaatata
     7201 ttgcagttat ctggtaggcc aggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc
     7261 agtctcagcg cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc
     7321 gcagtggtag catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt
     7381 attgagtaac tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc
     7441 tgaacagttg cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac
     7501 ctgggatgat tacacagaga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac
     7561 cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgcggaaa ttgtgattgt
     7621 cggcggcggt aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggctcc
     7681 aattactgac gtggttaaac gtggcgcgct gtatattggc tggagcgcag gcgctaacct
     7741 tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga
     7801 tgcgctaaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg
     7861 ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact
     7921 gacgattatt ggtctgccgg aaggtaactg gatcacagtg agtaaaggtc acgctacgct
     7981 gggtggcccg aacaccactt atgtgtttaa ggctggtgaa gaagcggttc cgctggaagc
     8041 tggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct
     8101 gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc
     8161 gtccttcatc ttcatccggc tgttccagca cactgtaagc caccgaacaa aacagcgagt
     8221 tcaggcgctg catatctccc agtaagccta aatgtagcga actggtttca atgctctgca
     8281 cgttttgctg atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa
     8341 aacgatgttt gctgcgacgc aaacgacgag cgctggtgac atcgccagag aagaacaccg
     8401 acattgccag ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc
     8461 catcaagcga aaatgcccgc cgtgctgcca gcgatttatc ggcaatttcg ctgcccatgc
     8521 gctcgacgat atcggaggcc tgttcaaggt tgagcgacat ttcgatgatc tccgcccagc
     8581 ggcgcgactc ttcctccgcc aactcttctt ttggcatccg cgccagatac agcttaatgg
     8641 cggtgtagag aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct
     8701 cgccgtgcat cactttattc agcccttcca tcatctgttc catggcgtcg ccaatgcgca
     8761 gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt gtccagcgcg ctgacatcca
     8821 gatgtttagg ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa
     8881 accgtgccat cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga
     8941 cgtggaaata gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca
     9001 gcaaatggac aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca
     9061 gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg
     9121 aaccgaggtt agctccaatc accagacaga gcgccacggg gaaggagata atgcctgcag
     9181 cggtcagagt cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca
     9241 tcgcgccaat cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa
     9301 cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa
     9361 tcagcccaag accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga
     9421 ggaaaaaaat cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga
     9481 gaatacgcgc cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg
     9541 cgagggctac cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc
     9601 tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc
     9661 tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa
     9721 tatgagtccc ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga
     9781 gttcagattc tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa
     9841 acacgctgac tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt
     9901 ccagcctgtt tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg
     9961 ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat
    10021 tcggtgccag ccagcgctca acatcggaga cgctcatacc tttacggcgg gcatggtctt
    10081 caacctgatc gcgctgaatt tgcgccaccg cgtagtactt gctgtccgga tgactgaaat
    10141 accaacccga aaccgatgca ccaggccaca tcgcgaaaga ttctgtgagt ttcatgccag
    10201 tatgtttttc cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg
    10261 ccggatagcc cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc
    10321 tgaggttctc gttcggcgca tagccccagt agactttacg cacacgctca tggagatact
    10381 ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat
    10441 cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa
    10501 atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt
    10561 agttagcgaa tcccgttttt tcggtctgtt gacgcagatg gtggctgacg ttgatcacat
    10621 gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca
    10681 ggcccaccac gccacgcgga ttcagcgttt tctcggcgct taatttatcc agcatgtcgt
    10741 tggcgtcttt aaacagccgc tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg
    10801 gatacttccc ggccagcgac caggtcataa agaacggtgt ccagtcgatg taattacgca
    10861 gcgtttcgat gctggcttcc acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt
    10921 aagcctgcca gtcaaatgcg aaatcgttat cgcgcgccgc ttccagcgtg accggtggtg
    10981 tgcgtggttt cttgcgcccg tgctgaatac gcacggtttc gtactccttg caggtacgag
    11041 cgacaaaatc atcacgctga gtatcggaaa gcagcgccgc taccacacca acggtgcgcg
    11101 aggcattctg cacatacacc gtagggccgc tgtagttctg ctcgattttc accgccgtgt
    11161 gcgcttttga ggtggtcgcg ccgccaatca gtaacggaat ggcgaagccc tgacgctcca
    11221 tctctttcgc cacgttaacc atctcgtcca gcgacggcgt gataagcccc gaaaggccaa
    11281 tcaaatcagc attcacttct ttggcgatac ggagaatttt ttccgcaggc accataacgc
    11341 cgagatcgac aatttcgtag ttgttacatt gcagcaccac accaacgata tttttaccga
    11401 tgtcgtggac atcgcctttc acggtggcga tcaccatctt gccgttggtt ttaccctgct
    11461 ctttgctggc ttcaataaac ggttcgagat acgccactgc ctgtttcatg acgcgcgccg
    11521 atttgaccac ctgtggcagg aacattttgc cttcgccaaa cagatcgccg accacgttca
    11581 tgccgtccat caacgggcct tcaatcactt caatcgggcg cgtagcctgc tggcgggctt
    11641 cttcagtatc ctgctcgata aactcggtta tgcctttgac cagcgagtat tccagacgtt
    11701 tattcacgtc ccacgagcgc cactccgctt gctgggcgtt agcagtgtcg tccgctttac
    11761 tgccgcgata tttctcggca agctccagta aacgctcagt gccatcgtca cgacgattaa
    11821 gaatcacatc ttccaccgcg tcgcgcagtt cagcgggtag gtcgtcgtaa atcgccagtt
    11881 gcccggcgtt gacgatcccc atatccatgc cattgcgaat agcgtagtag aggaacactg
    11941 cgtgaatggc ttcgcgcacc ggatcgttgc cacggaatga gaaagaaacg ttagatacgc
    12001 cgccggaaat cagcgcgtgc ggcagttcgc gtttgatgtc ttcacacgcg ccgataaagt
    12061 cctgcgcgta gttgttgtgc tcttcaatac cggtggcgac cgcgaagatg ttcgggtcga
    12121 agatgatatc ttctggcggg aagccaacct cttcggtgag gattttgtac gcccgacggc
    12181 aaatctcgat tttccgtgcg cgagtatcgg cctgtccctg ttcgtcaaag gccatcacca
    12241 ccactgccgc accgtagcga cgcaacaatt tcgcgtgatg gataaaggcg tcgacgccct
    12301 ctttcatcga gatagagtta acaatgcctt tgccctggat acacttcaga cctttttcaa
    12361 tgacatccca ttttgaggag tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg
    12421 caatcagatt gagaaaacgc accatcgccg cttcggcatc gagcatccct tcatccatgt
    12481 tgatatcgat aatctgcgcg ccgttttcca cctgctgacg cgccacatcc agcgcctcgc
    12541 tgtatttctc ttctttgatc aggcgcttga acttagcaga gccggtgacg ttggtgcgtt
    12601 cacccacgtt cacaaacagg ctatcgtcgc caatgttcag cggctccagg ccagacaaac
    12661 ggcaggctac gggaatttcc ggcagtttgc gcggcgctaa tccttctact gcacgactca
    12721 tcgctgcaat atgttgcggc gtggtcccgc agcaaccacc gacgatattg agaaagcccg
    12781 cttgcgccca ttcacgtatc tgttttgcca tcgtgtcggc gtcgagatcg tattcaccaa
    12841 aggcgttagg cagcccggcg ttcgggtggg cggtgacgta gcattccgca atccgtgata
    12901 gttcctgcac gtactggcgc agttcatcgg gccccagcgc acagttcagg ccaaaggtca
    12961 gcgcttcggc gtggcgcaat gagttgtaaa atgcttcggt ggtctgcccg gagagcgtgc
    13021 gcccggaggc gtcggtgatg gtgccggaga tcataatcgg cagctcaacg cccagcgctt
    13081 caaactccgt tttcaccgca aagaccgccg ctttggcgtt aagggtgtcg aaaacggttt
    13141 caatcaggat cagatccgca ccaccttcca ccagcgcttt ggtggattcg cgataggccg
    13201 ccgccagctg gtcaaaagtg atgttgcgga atgccggatc gttgacgtcc ggagaaatag
    13261 acgccgtgcg gttggttggg ccaagaacac cggcaacgta gcgcggtttc tccggcgtgc
    13321 gcgcggtcca ctcgtcagca caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg
    13381 ccgacaggga ttccatctgg taatccgcca tcgcaatggt cgtggagttg aaggtgttgg
    13441 tttcgatgat atccgcgccc gcttcaaagt aggcgttgtg gatagcggcg atcacttccg
    13501 gtttactgag taccagcagg tcgttgttgc ctttgaggtc gcatggccag tcggcaaagc
    13561 gttcaccacg aaaatcggct tcgttcagtc gatagctctg gatcatggtg cccataccgc
    13621 cgtccaacac cagaatacgt tcatttaact gcgcacgcag ttgttccact ttgctgctca
    13681 cacttgctcc cgacacgctt aacccagatt taataaaaat tcaacaaacc atactggaat
    13741 aaacgcatct gtggtaaaag cgaccaccac gcaacatgag atttgttcaa cattaactca
    13801 tcggatcagt tcagcaacta ttgcattagc taacaataaa aatgaaaata atttccacga
    13861 tacagaaaaa ggaggctgtc atggtcgcac ccattcccgc gaaacgcggc agaaaacccg
    13921 ccgttgccac cgcaccagcg actggacagg ttcagtcttt aacgcgtggc ctgaaattac
    13981 tggagtggat tgccgaatcc aatggcagcg tggcactcac agagctggcg caacaagccg
    14041 ggttacccaa ttccacgacc caccgcctgc taaccacgat gcaacagcag ggtttcgtgc
    14101 gtcaggtcgg cgaactggga cactgggcaa tcggcgcaca tgcctttatg gtcggcagca
    14161 gctttctcca gagccgtaat ttgttagcaa ttgttcaccc tatcctgcgc aatctaatgg
    14221 aagagtctgg cgaaacggtc aatatggcgg tgcttgatca aagcgatcac gaagcgatta
    14281 ttatcgacca ggtacagtgt acccacctga tgcgaatgtc cgcgcctatc ggcggtaaat
    14341 tgccgatgca cgcttccggt gcgggtaaag cctttttagc ccaactgagc gaagaacagg
    14401 tgacgaagct gctgcaccgc aaagggttac atgcctatac ccacgcaact ctggtgtctc
    14461 ctgtgcattt aaaagaagat ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg
    14521 aggaacatgc gctggggcta cgttgccttg cagcgtgtat tttcgatgag caccgcgaac
    14581 cgtttgccgc aatttctatt tccggaccga tttcacgtat taccgatgat cgcgtgaccg
    14641 agtttggcgc gatggtgatt aaagcggcga aggaagtgac gctggcgtac ggtggaatgc
    14701 gctgactttt tctggcgggc agaggcaata ttctgcccat catacctgag tggcaataga
    14761 ataagggtgt ctgttaatcg cattgacgcc aaaataactt aatgtcatac acttcacaca
    14821 cttttattaa ctcagcatta tttttaaaca tcaaaccact taattattaa caaaatcgca
    14881 agatctcatt ccaccgaaac ttttacggct gcattatgag attgagcgat ttatgatatt
    14941 tttttagttg taatcatatt aaattttttc actgttctga tagttaaaat tcaagacatc
    15001 aataaacaat gagatattta aatgattact cgtattcctc gtagttcttt ctctgcaaat
    15061 attaataata cagcccagac aaatgaacac caaaccctga gtgaattgtt ttataaagaa
    15121 ctcgaggata aattttctgg caaggagctg gcgacgcctc tattaaaaag cttctcagag
    15181 aactgtagac aaaatgttcg tcatatcttt agcaacaagg attttgtcat taaattttcc
    15241 acgtccgtct tacaagctga taagaaagaa attacgatga ttaataaaaa cgaaaacacg
    15301 acactcactc aaaccattgc tacaatattt gaagaatacc taatggaaat tttacctcaa
    15361 cgctcagaca ctcttgataa acaagaatta aaccaaaaat cagatagaaa agaaaaagaa
    15421 ttcccaagaa ttaaacttaa tggtcaatgt tatttcccgg ggcgccccaa aaccgtatag
    15481 tataccgaca cattgctgca caatatatta atgatattta tcagaatgtt gattacaaac
    15541 cccatcaaga tgattactct tcagctgaaa aatttctcac tcacttcaac aaaaaatgca
    15601 aaaaccagac tttggcgttg atttccagcc gtcctgaggg gcgttgcgtt gctgcctgcg
    15661 gtgatttcgg gctagttatg aaagcatatt ttgacaagat ggaatcaaat ggcatcagtg
    15721 ttatggcagc catattactg gtggataacc atgctttgac ggtccggcta agaataaaga
    15781 acacaactga aggatgtacc cattacgtgg tttcggttta tgatcctaat gtaactaacg
    15841 ataaaataag aattatgagc gaaagcaaag aggatattaa acactattct ctgatggatt
    15901 ttatgaatgt agattatagc ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg
    15961 ttgcaataat tccagcactt ccgaaagaac agctattgat gttaaaagga actgtggatg
    16021 aaataacccc tccattatca ccagcaacga tgaatttgct aatggcaatt ggtcagaatc
    16081 accaacttac gcaactgatg attcagctcc agaaaatgcc agaactacat agaacagaaa
    16141 tgttgactgc ctataatagt attaacctcc ccggtttata tttggctata aattatggca
    16201 atgcggatat cgttgagact attttcaatt cattgtcaga aacaggatat gaagggttac
    16261 tctcgaaaaa aaatctcatg catattctgg aggcaaaaga taaaaatggt ttttctggat
    16321 tatttttagc gatatcacgt aaggataaaa atgttgtaac ctcgattctg aacgtcttac
    16381 ctaaactggc cgcaacacat catttagata atgaacaggt gtataaattc ctgagtgcca
    16441 aaaatagaac gtccagccat gttttatacc atgttatggc gaatggtgat gccgacatgc
    16501 tgaaaatttt tttggtcgcg ttacctttgt taattcgcac atgtcatttg actaaagaac
    16561 aggtactcga tctcctgaag gcaaaggatt tttatggttg cccaagacta tacctggcga
    16621 tgcaaaatgg acatagcgat atcgtgaaag ttattctcga agcattgccc tgtctggccc
    16681 aggaaattaa catttcagct tccgatattg tagatcttct gaccgctaaa agtcttgcgc
    16741 gcgacacggg tttgtttatg gccatgcagc gcggacatat gaacgttatt aatactattt
    16801 ttaacgcatt acccactctt tttaatacgt ttaaattcga taaaaaaata tgaagcccct
    16861 cctcctggca aataattcta atgaatatcc cggtttgttt tcagcgatac agcataaaca
    16921 acaaaacgtt gtagagacgg tttatcttgc tttatctgac catgcacgcc tgtttggatt
    16981 taccgctgaa gatattatgg atttttggca acacaaagcg ccacaaaaat actctgcctt
    17041 tgagttggct tttgaatttg gtcaccgggt tattgctgaa ttaatcctta atacattaaa
    17101 taagatggct gaaagctttg gctttacgga taaccctcga tacattgcgg agaaaaatta
    17161 tatggaagct ttactcaaaa aagcatctcc ccataccgta cgctaaatct ttgcctgcgc
    17221 cgatacgcat aaacatcttc cacatgccct tcacgtatgc ggttttgtag tgcgcgccag
    17281 taatcagcgc ggaacaggtc ggcgtgcatc tcttcaaaca gcggaccaat acgcgggtcg
    17341 gcgcatagcc agtggcgaaa ctcttccggg aaaacatcgc ccggcgagac gctgtaccac
    17401 ggttcgctgg caagttcgtc ttccggatag cgcggcggcg ggatgtcgcg gaaattcact
    17461 tccgtcatgt agcaaatttc atcgtaatca taaaaaacca cacgcccgtg acgggtgaca
    17521 ccgaagtttt taaacagcat gtcgccaggg aaaatgttag cagcggcaag ctggcgaata
    17581 gcgttaccgt attcttcaat ggcgtcgcgc aactgctgac cttccacttg ttccagccag
    17641 atattgagcg gcaccatccg ccgctcgatt atagagatgg cgaattacaa tctgctcacc
    17701 aagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaata ccggggaaat
    17761 atgccgcttc tccagcacaa agttttcaaa ctcctgggtg tccgccattc ggcccacacg
    17821 gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca tctctttctg
    17881 cggcgcgaac ttgtctttga tgactttgaa tacccgatca aagcccggca gcgtaaacac
    17941 caacatcacc attccacgaa tacccggcgc ttcaatgaac tgctcattac agccctgtag
    18001 ataaacgaga tattcgcggt agctttcggt tttggcgtgc ttctggcagc cgatagccat
    18061 atacaattca gcagtggttt tacctggcag aatttcccgt agccactcga ccagcgctgc
    18121 gggcagcggt gcataaacca taaaataaga acgcgcaaag ccaaaaacaa tgctcgcttc
    18181 ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg tcgtccgtct ggtggatcgg
    18241 cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg cccaccagcc aggcggcttt
    18301 attgcggtaa aacagttcgt tcgccacctg taaatgactt tccgcgaggt tgtctgtccc
    18361 cagcgtttcc gtcagatggc gaatgatgta atgaatgtca cggcttttat tctgccagcg
    18421 caggcgcagc ggcaggtcgc tgataacgcg catcagtaga gattcccagc cgtgatcggg
    18481 gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag cggcgctctg gctgagagct
    18541 aaagataaaa agccgctcgg gagtaagcga gcggtggtca aataaccgac agtacacgga
    18601 gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc ggcaacagcc gcgtgtaatg
    18661 ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg ccgttagtaa tgcagcgcag
    18721 ttgctccacg accagaccaa cgtgatgatc gtaaagatgg atacggtttt tcatcgcctg
    18781 ctggacagca tgccagtcgg cctgttcgaa acgctgctgc gccccggaag tcacttcgag
    18841 gaatcgacca tactgagcgt cgaagccttg caaaatggtt tgagcaatca gtaattccag
    18901 gccacgcggc atttagcgcc ctcatcagga gcagagaatt gccggatgcg gcgtaaacgc
    18961 cttatccagc ctacgttcgg caacggttgt aggcatgata agacgcgcaa gcgtcgcatc
    19021 aggcatcgtg caccgattgc cggatgcggc gtaaatgcct tatccggcct acagtcagca
    19081 acgtttgtag gttgcttaga actgcgattc ttcagtggag ccagtcagcg cggtgactga
    19141 agacgtgccg ccctgaataa tagtcgtcac tttatcgaag taacctgtac ccacttcctg
    19201 ctggtgagat acgaaggtat agccatcttt cgcggcggca aattccggct gctgcacttt
    19261 ctcaacatag tgcttcatac cttcgccctg cgcataagcg tttgccaggt cgaacatgtt
    19321 aaaccacatg ctgtggatac ctgctagggt gatgaactgg aacttgtagc ccatatccga
    19381 cagctgctgc tggaagctgg caatagtttt gtcgtcgagg tttttctgcc agttgaacga
    19441 cggcgagcag ttataagcca gcagtttgcc cggatatttc gcgtggatag cttgtgcaaa
    19501 gcgacgcgcc agttccagat ccggcgtgga ggtttcacac cagaccaggt cagcatatgg
    19561 cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc
    19621 ttcactggta cgctcgccgg taataaattc gctgtcatac gggtcgcaat ctgaggtgat
    19681 cagatccgcc gcatcagcat cggtacgtgc aaccagcagg gttggaacgc ccgtcacgtc
    19741 agctgccaga cgcgccgcga ccagtttctg aatagcttcc tgagttggca ctaaaacttt
    19801 gccgcccatg tgaccgcatt tcttcactga cgccagctga tcttcgaagt gaactgccgc
    19861 agcaccggct tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc
    19921 ggcttccgca tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc
    19981 aatgcccacg gaccattgga tctgatcggc acgacggaag gtgttgttga tccgctccac
    20041 cacagctggc accgagtttg ccggatagag cgactgatcc ggatacatgc tggccgccag
    20101 gttagcgtcc gccgctacct gccatcccga cagatagact gcttcaatac ccgctttcgc
    20161 ctgttgcagc gcctgaccgc cagtcagtgc accgaggttg ttgatgtagc cttttttcga
    20221 ctcaccgtgc agcagacgcc acattttcgc tgcgcccagt tgcgccagcg tgcattcagg
    20281 attgactgaa ccgcgtaatt tcaccacatc ttccgcactg tatgggcgag taatgccttc
    20341 ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat
    20401 gtgcagatgc tccatagtta tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc
    20461 aggaaatcaa ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca
    20521 aaacggccct gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttcg
    20581 cccagcatct ggcggaacaa ggctttggtc accggtttgc cattgctcaa cgttttttga
    20641 tgatggatcc actgccagat cgaggtacgg gaaatttcag ccgtcgccgc atcttccatc
    20701 aggccataaa tcggcacgca gccgttgccg gagatccacg cttcgatgta ctgcacagcc
    20761 acgcgaatgt tagcgcgcat accttcttcg gtgcgttcac catcacaagg tgccagcagc
    20821 tgatcggcag taatcggcgc gtcttgttcg cgcaatactt caagctgatt tttacgggag
    20881 ccgagaatgt cgttgaatac cgccattgcc gtgtccgcaa ggcctgggtg agcgatccat
    20941 gtgccatcgt gaccgttatt ggcttccagc gctttatccg cttttacttt gttgagcacc
    21001 tggttattac gctcttcatc tttgctcgga ataaacgcgg ccatgccgcc catcgcaaaa
    21061 gcaccgcgtt tatggcaggt tttaatcagc aggcgtgagt aagcattcag gaatggttta
    21121 tccatcgtca cggcctgtct gtctggcagg acacgatcgg gatagttttt caacgttttg
    21181 atatagctga agatgtaatc ccagcgaccg cagttcagac caacaatatg gtcgcgcagc
    21241 gcgtgaagga tttcgtccat ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc
    21301 ttgatggtgc cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac
    21361 caggccgctt cctgctagga ctgggttttc ggcagataga aatagggacc gctgcccttt
    21421 gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctaccgggg
    21481 attgcctcac cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa
    21541 atcaaaaccg ctggattggg cttgagctga taaattttgc ctgcttcatt ggtatagctg
    21601 atggtaccgt taaccgcatc gcgcaggtta atttgcccgt cgatcacttt gttccagtct
    21661 ggtgccagtg aatcttcgaa atcggccata aagactttca cattggcgtt cagtgcgttg
    21721 atcaccatct tgcgctctac cgggccagtt atctctacgc ggcggtcttc taagtccgca
    21781 ggaatcccgc gaattttcca atcagcatcg cgaatggaag ccgtttccga aataaaatca
    21841 ggcaacgttc cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattg
    21901 cgttgtggcg taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga
    21961 atttgcttct cctgctcgcc atacggcctt gtgaaagcca gttcatcggt tgttgttgcc
    22021 tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa cttcccctct gtgaacgaag
    22081 gaacgttgtg cgcttttcgt tcagcacaac taagactact catttaaaat gcaaaatcaa
    22141 aaacaatttc cattttaatt aaaaatactt gataacttat tgataacaat atgaataaaa
    22201 tttattcact tgctgaggtg cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt
    22261 aaggtgctga atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat
    22321 gtgccgtaga tcgtatggcg tgatctgata gacgtaatag ttgagccagt tggtaaacag
    22381 caaattaccg tgactacgcc agctcgctcg cggtttgttt tgcggatcgt tgtgcgggaa
    22441 atagttatac ggtacatccg ggtctagtcc ggcttccaca tcgcgtaaat attcctgcgc
    22501 cagcgtttgc gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttgct
    22561 ggcaaacaga tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc
    22621 acgaatcaac gctgccggaa agtcagcata gcgcgaatgc ggtgccagga atgaatcatc
    22681 aaagccacgc gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag
    22741 tttgtcggtg cgagtttgct taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc
    22801 ccagcagaca aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat
    22861 ctgcggccag taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat
    22921 cagaccgtca aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg
    22981 ctctgcgggc gtgttgcgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg
    23041 caaaggtgag tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat
    23101 caggttaagg atcagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt
    23161 catcacaaag acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg
    23221 aatcggcata acctgattac ctcactacat acgcttatac gtttagacat ccagatagct
    23281 gaagataacc agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa
    23341 ggaaaaggct acgttagaat ataagaatga cgaaaaggag agaaaatggt tattagtatt
    23401 cgccgctcac ggcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat
    23461 gccacccacg attttttatc agcagagtat cgggccgagc tggaagagct ggtgcgttcc
    23521 tttctgccgg aagcaccgtt gtgggtcgcg gttaatgaac gggagcagcc ggttgggttt
    23581 atgttgttaa gtgggcagca tatggatgcg ctgtttatcg atcctgatgt gtgcggctgc
    23641 ggcgtgggtc ggatgctggt ggagcatgcg ctctcgatgg caccggaact gacaaccaac
    23701 gttaatgagc aaaatgagca ggcggttggg ttctataaga aggtgggttt taaggttacg
    23761 ggacgctctg aggtggacga tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg
    23821 ggggaataag ccgcctctgc cgtgcctgcc caagctggag aagacg
//
LOCUS       DAJTPG010000059        22958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTPG010000059 DAJTPG010000000
VERSION     DAJTPG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OO230_002416"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OO230_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5692137.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OO230_002417"
     CDS             1207..1869
                     /locus_tag="OO230_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5692138.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OO230_002418"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OO230_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5692139.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OO230_002419"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OO230_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OO230_002420"
     CDS             2795..3100
                     /locus_tag="OO230_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5692140.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OO230_002421"
     CDS             3103..3507
                     /locus_tag="OO230_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5692141.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OO230_002422"
     CDS             3497..3796
                     /locus_tag="OO230_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5692142.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OO230_002423"
     CDS             3982..4278
                     /locus_tag="OO230_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5692143.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OO230_002424"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OO230_002424"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5692144.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OO230_002425"
     CDS             5627..5995
                     /locus_tag="OO230_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5692145.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO230_002426"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="OO230_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5692146.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="OO230_002427"
     CDS             7203..7904
                     /locus_tag="OO230_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5692147.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="OO230_002428"
     CDS             7927..8091
                     /locus_tag="OO230_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692148.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="OO230_002429"
     CDS             complement(8303..9613)
                     /locus_tag="OO230_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5692149.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="OO230_002430"
     CDS             complement(9641..10972)
                     /locus_tag="OO230_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5692150.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO230_002431"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="OO230_002431"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5692151.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="OO230_002432"
     CDS             complement(12683..13072)
                     /locus_tag="OO230_002432"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5692152.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO230_002433"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="OO230_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5692153.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO230_002434"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="OO230_002434"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5692154.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO230_002435"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="OO230_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5692155.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO230_002436"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="OO230_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5692156.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO230_002437"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="OO230_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5692157.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="OO230_002438"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="OO230_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5692158.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="OO230_002439"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="OO230_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="OO230_002440"
     CDS             21379..21726
                     /locus_tag="OO230_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692159.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21895..22545
                     /locus_tag="OO230_002441"
     CDS             21895..22545
                     /locus_tag="OO230_002441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692160.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6195 a         5786 c         5291 g         5686 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
    22921 gcgggttttt acgtatatca ggtgaaactg accgataa
//
LOCUS       DAJTPG010000060        22430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTPG010000060 DAJTPG010000000
VERSION     DAJTPG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            23..232
                     /gene="fumD"
                     /locus_tag="OO230_002442"
     CDS             23..232
                     /gene="fumD"
                     /locus_tag="OO230_002442"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5692161.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            688..1314
                     /locus_tag="OO230_002443"
     CDS             688..1314
                     /locus_tag="OO230_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5692162.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1335..3436
                     /locus_tag="OO230_002444"
                     /pseudo
     CDS             1335..3436
                     /locus_tag="OO230_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3449..4087
                     /locus_tag="OO230_002445"
     CDS             3449..4087
                     /locus_tag="OO230_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5692163.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4151..4819
                     /locus_tag="OO230_002446"
     CDS             4151..4819
                     /locus_tag="OO230_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5692164.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4816..5601
                     /locus_tag="OO230_002447"
     CDS             4816..5601
                     /locus_tag="OO230_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5692165.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5605..6417
                     /locus_tag="OO230_002448"
     CDS             5605..6417
                     /locus_tag="OO230_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692166.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6429..7851)
                     /locus_tag="OO230_002449"
                     /pseudo
     CDS             complement(6429..7851)
                     /locus_tag="OO230_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7977..8282)
                     /locus_tag="OO230_002450"
     CDS             complement(7977..8282)
                     /locus_tag="OO230_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5692167.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8390..8466)
                     /locus_tag="OO230_002451"
     tRNA            complement(8390..8466)
                     /locus_tag="OO230_002451"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8430..8432),aa:Val,seq:gac)
     gene            complement(8471..8547)
                     /locus_tag="OO230_002452"
     tRNA            complement(8471..8547)
                     /locus_tag="OO230_002452"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8511..8513),aa:Val,seq:gac)
     gene            8855..10111
                     /locus_tag="OO230_002453"
     CDS             8855..10111
                     /locus_tag="OO230_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5692168.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10152..11525)
                     /gene="mdtK"
                     /locus_tag="OO230_002454"
     CDS             complement(10152..11525)
                     /gene="mdtK"
                     /locus_tag="OO230_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5692169.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11740..12381
                     /gene="ribE"
                     /locus_tag="OO230_002455"
     CDS             11740..12381
                     /gene="ribE"
                     /locus_tag="OO230_002455"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5692170.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12421..13569)
                     /gene="cfa"
                     /locus_tag="OO230_002456"
     CDS             complement(12421..13569)
                     /gene="cfa"
                     /locus_tag="OO230_002456"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5692171.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13860..15071)
                     /locus_tag="OO230_002457"
     CDS             complement(13860..15071)
                     /locus_tag="OO230_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5692172.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15184..16116
                     /locus_tag="OO230_002458"
     CDS             15184..16116
                     /locus_tag="OO230_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5692173.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(16113..17138)
                     /gene="purR"
                     /locus_tag="OO230_002459"
     CDS             complement(16113..17138)
                     /gene="purR"
                     /locus_tag="OO230_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5692174.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17437..17526
                     /gene="ynhF"
                     /locus_tag="OO230_002460"
     CDS             17437..17526
                     /gene="ynhF"
                     /locus_tag="OO230_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5692175.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17692..18861
                     /locus_tag="OO230_002461"
     CDS             17692..18861
                     /locus_tag="OO230_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692176.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19007..19588)
                     /gene="sodB"
                     /locus_tag="OO230_002462"
     CDS             complement(19007..19588)
                     /gene="sodB"
                     /locus_tag="OO230_002462"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5692177.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19716..20543)
                     /gene="mepH"
                     /locus_tag="OO230_002463"
     CDS             complement(19716..20543)
                     /gene="mepH"
                     /locus_tag="OO230_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5692178.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20878..21225
                     /gene="grxD"
                     /locus_tag="OO230_002464"
     CDS             20878..21225
                     /gene="grxD"
                     /locus_tag="OO230_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5692179.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21264..21764)
                     /gene="tnpA"
                     /locus_tag="OO230_002465"
     CDS             complement(21264..21764)
                     /gene="tnpA"
                     /locus_tag="OO230_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5692180.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21772..>22430
                     /locus_tag="OO230_002466"
     CDS             21772..>22430
                     /locus_tag="OO230_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5692181.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         5813 a         5216 c         5807 g         5594 t
ORIGIN      
        1 gcatgataaa taggtataaa gaatgggaaa tcggacaaaa gaggatgaac tgtaccgaga
       61 aatgtgcaga gttgtcggta aagttgtgct ggaaatgcgc gatctggggc aggaaccaaa
      121 gcatattgtt atcgcaggcg tattacgtac agcattagcc aataaacgca ttcagcgcag
      181 tgagctggaa aagcaggcga tggaaaccgt cattaacgca ctggtaaaat gaccattcag
      241 gatgaggcgg taagcttcat cctgatattc ttgcttcagt tgcggtccag ttaatcattc
      301 taaataacaa ctgctgaaat tatacgaatt tcggtatgta ttttaattta tagcgataat
      361 gttattttta ttttctgcaa tgaacgtcac aaatgattgt cgcttaagtt aaatttattt
      421 caaatgataa taaatatcag ttcgcttata atttcttgct ttgtaatttt aattgataac
      481 gatcaatgtt aagggctcag aataatcaca ttattttttc tcctaaccat aaaggattag
      541 tttatcggcg ttaaatcttt tctcatacac tatcgggtta gggaaaatat ctggtgttgg
      601 ggacagagtc agcagcgtat tagttgaatg cttaatatca ggctgctgaa ttgtcagcac
      661 tgtgtttgcg aagaggatgc gaaataaatg aacccggttg atcgtccact attagatatt
      721 ggcttaacgc gattagaatt cttgcgtata tcaggaaaag gccttgctgg tctaacaatt
      781 gcccctgcgt tactgtcact tttgggttgc aaacaggaag atattgatag cggcacagta
      841 gggttgataa atacacccaa aggggtgctg gtgacccagc gagcgcgatg cacgggttgc
      901 caccgctgtg aaatctcttg taccaacttc aacgatggct cagtagggac attcttctcc
      961 cgtatcaaaa tccatcgcaa ttatttcttt ggcgacaacg gggttggctc tggcggcggc
     1021 ctgtatggcg atctcaacta taccgcggac acctgccgtc aatgcaaaga gccgcaatgc
     1081 atgaacgtct gcccgattgg tgcgattacc tggcagcaga aagaaggctg tattaccgtc
     1141 gatcataaac gttgtattgg ctgtagcgcc tgtactacgg catgcccgtg gatgatggcc
     1201 accgtaaata ccgaaagtaa aaaatcctcg aaatgtgtgt tatgcggtga atgcgcaaac
     1261 gcctgcccaa caggggcgtt aaaaattatc gagtggaaag atattactgt gtgaatcttg
     1321 caaaggaaaa aatcatggct aacggttgga caggtaatat attaagagtc aatctcacga
     1381 caggaaatat taccctcgaa gattccagta agtttaaaag ttttgtcggt ggcatgggct
     1441 tcggctacaa aattatgtat gacgaagtac cgctaggcac gaaacctttc gatgaagcga
     1501 ataaattagt ctttgctacc ggcccattac ctggatctgg tgccccctgt agttctcgcg
     1561 taaatatcac ctcactttct acttttacca aaggaaattt agtcgcgatg cccatatggg
     1621 tggctttttt gcagcgcaaa tgaaattcgc tggatacgac gtcattatta tcgaagggaa
     1681 ggcgaaatca ccggtatggc tgaagattaa agatgacaaa gttagcctgg aaaaagccga
     1741 tttcttatgg ggaaaaggga cgcgcgcaac gacggaagaa atttgtcgat tgaccagccc
     1801 ggaaacctgt gtggcggcta ttggtcaggc tggggaaaac cttattcctc tctctggcat
     1861 gttgaatagc cgtaaccaca gcggcggtgc gggaactggc gcaataatgg gttcgaaaaa
     1921 cctgaaagcg attgcggttg aagggacgaa aggggtcaac attgccgatc gtcaggagat
     1981 gaagcgtctc aatgattaca tgatgactga acttattggt gcgaataaca accatgtcgt
     2041 gccaagtacg ccacaatcgt gggcagagta ttcagatccc aagtcacgct ggacagcacg
     2101 taaagggctg ttttggggcg cggctgaagg tggtccgatt gaaacgggtg aaattccgcc
     2161 aggcaatcag aatacggtcg gctttcgtac ctataaatcc gtttttgact taggaccggc
     2221 ggcagagaaa tacacagtaa aaatgagcgg ctgccactct tgcccgatcc gttgtatgac
     2281 ccaaatgaat attcctcggg tgaaagagtt tggcgtgccc agcacaggtg gtaacacttg
     2341 tgtagcaaac tttgtccata ccaccatctt cccgaacggg ccgaaagatt ttgaagataa
     2401 agacgatggt cgtgtgattg gtaacctggt tggtctgaat ttgttcgatg actacggcct
     2461 atggtgtaac tacgggcagt tgcatcgcga ctttacttat tgttacagca aaggtgtgtt
     2521 caagcgtgtt ctgccagctg aagagtatgc agaaattcgc tgggatcaac tggaagcggg
     2581 tgacgttaac ttcattaaag atttttacta ccgtctggcg catcgtgtgg gtgagctgag
     2641 tcacctggct gatggttcat atgccatcgc agaacgctgg aatttgggta aagagtactg
     2701 gggctacgcg aaaaataaac tctggtcgcc gtttggctat ccggttcacc atgccaatga
     2761 agcgtcagcg caggtcggtt ccattgttaa gtgtatgttc aaccgtgact gcatgacgca
     2821 tacccatatc aactttattg gttccggctt gccattgaaa ctgcaacgtg aagtggcgaa
     2881 agaacttttt ggttctgaag atgcttacga tgaaaccaaa aactacacgc caatcaatga
     2941 cgcaaaaatt aaatatgcaa agtggtcgct gttgcgggtc tgtttgcata acgccgtcac
     3001 tctgtgcaac tgggtctggc caatgaccgt ttcgccgctg aaaagccgta attaccgggg
     3061 cgatctggcg cttgaagcca aattcttcaa agcgatcacc ggcgaagaga tgactcagga
     3121 aaaattagat ttagctgcag agcgtatctt tacgttgcat cgtgcctaca cggtaaaacc
     3181 gatgcaaacc aaagatatgc gtaatgaaca cgatcttatc tgttcctggg ttttcgacaa
     3241 ggatccgcag atcccggtct ttactgaagg tactgacaaa atggatcgtg acgatatgca
     3301 tgcttcgctc acgatgttct acaaagaaat gggctgggac ccacagcttg gttgtccaac
     3361 ccgcgaaaca ttgcagcgtc tggggctgga agatattgcc gccgatctgg cagcacacaa
     3421 tctactgcct gcgtaaggaa tgggtaagat gaaccatcag gatgaattgc cgttagcgaa
     3481 agtcagcgaa gttgacgagg cgaagcggca atggttgcag gggatgcgtc accccgttga
     3541 tacggtaacc gaaccagaac ctgctgaaat cctggctgag ttcattcgcc agcattctgc
     3601 ggctgggcag ctggtggcgc gcaccgtgtt tctctcgcca ccctattcgg ttgcagaaga
     3661 ggaactgtca gttttgctgg aaagcattaa gcagaacgga gactacgcgg atatcgcttg
     3721 tatgacggga agtcaggatg attactatta ctcaacgcag gccatgagtg aaaactacgc
     3781 ggcgatgtct ttgcaagttg tggagcagga catttgtcgc gccattgcac acgccgttcg
     3841 ttttgagtgc cagacgtatc ctcgtcctta caaagtcgcc atgctgatgc aagcgccata
     3901 ttacttccag gaagcgcaga ttgaagccgc gatcgccgcg atggatgttg cacctgaata
     3961 tgccgatata cggcaggtgg agtcatcgac ggcggtgttg tacttgttta gcgagcgatt
     4021 tatgacctat ggcaaggctt atggcttatg cgaatggttc gaggtagagc agtttcagaa
     4081 cccctgaaat ttcagggaga tgtcatggat agggtggaca gttgcagcca ctgcattagg
     4141 ggataatcag atgtccttca ctcgacgcaa atttgttctg gggatgggaa cagtaatctt
     4201 ttttactggt tctgcgtctt ctctgttagc gaacacgagg caagaaaagg aagttcggta
     4261 tgccatgatt catgacgagt cacggtgcaa tggctgtaat atttgcgccc gcgcatgccg
     4321 taaaactaac cacgtccccg ctcagggaag ccgtttatcg atagcacaca ttccagtcac
     4381 cgataacgac aacgaaacgc agtatcactt ttttcgccag tcatgtcagc actgcgaaga
     4441 tgctccgtgc atcgacgtat gcccgacggg ggcatcgtgg cgtgatgagc aggggatcgt
     4501 gcgggtagaa aaatcgcagt gcatcggctg cagttattgc attggcgctt gtccgtacct
     4561 ggttcgctac cttaaccctg tcactaaagt ggcagataaa tgtgatttct gcgctgagtc
     4621 ccgactggca aaaggctttc cgcccatttg cgtcagcgcc tgtccggaac acgcattgat
     4681 ctttggtcgc gaagatagcc cagaaattca ggcgtggttg caggataata aatactatca
     4741 atatcagcta cctggtgccg gaaaaccgca tctgtatcgc cggttcggtc aacatttgat
     4801 taaaaaggaa aatgtatgaa cccgtcgcaa catgctgaac agtttcagag ccagttagcg
     4861 aactatgtgc cacagttcac tcccgaattt tggccggtgt ggttgatcat tgccggagta
     4921 ttgctggttg ggatgtggtt ggtgctgggg ctgcacgcct tgcttcgtgc tcgtggcgtg
     4981 aagaaatcag ccaccgatca tggtgagaag gtttatcttt acagcaaagc ggtcagatta
     5041 tggcactggt cgaatgcgtt actctttgta ttgttgctgg caagtgggct gataaatcac
     5101 tttgcgatgg tgggcgcaac tgcggttaaa agtctggttg cggtgcatga agtttgcgga
     5161 tttttgttac tggcatgctg gctcggcttt gtgctgatca atgccgttgg ggataatggt
     5221 caccactatc gcattcgtcg tcaggggtgg ctggaacgag cggcaaaaca aacgcgattt
     5281 tatttgtttg gcattatgca gggggaagaa catcctttcc cggcaacaac acagtctaaa
     5341 tttaatccct tacagcaggt cgcctatgtt ggtgtcatgt atggattgct gccgttgtta
     5401 ctattgacgg ggctgttgtg tctctatccg caagccgtgg gagatgtgtt tcctggcgta
     5461 agatactggt tattgcaggc gcattttgct ctggcattta taagcctctt ttttatcttc
     5521 ggtcatcttt atctttgcac cacggggcgt acgccacacg aaacctttaa aagcatggtc
     5581 gatggctatc accggcacta acgaatgatc attacacgag cagacctgcg agaatggcgt
     5641 atcggagcgg tgatgtatcg ctggttcctg cgccatttcc cacgcggcgg tagttatgcc
     5701 gatattcgtc atgcactgat tgaggaagga tatacggact gggcggagag tctggttgag
     5761 tatgcgtgga aaaaatggct ggcggatgaa aacttcgcgc atcaggaagt ttcctctatg
     5821 caaaaactgg ccacagaccc cggagagata ccattttgca gtcagtttgc ccgcagcgac
     5881 gatcatgctc gcatcggctg ttgtgaggat aacgcccgaa tcgcaaccgc tgggtatgcc
     5941 gcgcaaattg ccagtatggg ttatagcgtg cggattggaa gtgtcggctt taacagtcac
     6001 attggcagct ccggtgaacg cgcacgggtt gctgtaaccg gtaattcttc gcggataagc
     6061 agcgctggag atagtagtcg gattgcgaat accggaatgc gagtacgcgt ttgtacattg
     6121 ggtgaacggt gtcatgtcgc cagtaatggt gacctggtgc aaatcgccag ctttggcgca
     6181 aacgccagaa ttgccaacag cggcgacaat gtacacatca ttgccagcgg tgaaaattca
     6241 acggtagtga gcacgggcgt ggtcgactca attattctcg ggctaggggg aagcgccgca
     6301 ctggcatatc atgacggcga aagagttcgc ttcgccgtcg ccattgaagg tgaaaacaat
     6361 attcgaaccg gagtccgcta tcgtcttaat gaacagcatc agtttgttga gtgctgatag
     6421 ctttaccttt aatcaaacga aagacgccga cgagcacggg atgcaggctt tacgaccgcc
     6481 cgagccatcg cctcaccaat ttctgcacat gccgtaagtc cctgaacgaa aggctggtcg
     6541 tgcatcaacc agggcaacgc gccgaacgct acgcgtccac gaatatcttc gggttgctgt
     6601 aacgtataat cttcgccaac atcagggatt tcagatcccc gcttcacgta aggccagtag
     6661 tcgacgaata gactctgaag ggattgccgc ataattatcg atgaaaaccc tggcaaggcc
     6721 tttactgaac cgtttatggt cgtgttcatt cagatgagga acaatttcct gtacggcttc
     6781 atgcaggcgc agaatgacat aacgccaggg aacagtatgt ttttctcgtt tattgcgttc
     6841 gacttcctgg agattttttt ctgcccagtc aaatgggtcg cgttgtttgc gctcggcaaa
     6901 ccaggcttga gcaaaggaat cgacattcag gctctctaag gctatgcgtt ggctccagtc
     6961 tggatcagca aacttgattt cctctactat caatctaaat acccgatcca aaaggccctc
     7021 ttcacctttt tgaatctcag catttaatgc ctgatcggtg acgatgtgta agggctcgta
     7081 gggaataggg caatagaaat cggcttcggg taaaataccc gtgcgcgaca tcaacgtaat
     7141 atttagcttt tcacttgcgt tatcgcggtg aaaaacgacg tgttgtttat catcttcaat
     7201 gaacgaacca tgctgaatag ccactgccat tgccgcatcc agtccgctca aggatgttcc
     7261 cataataccc acgttacacg catcgacctt tgcttccatc aggcctgacc acgggctggg
     7321 aaaatacgtt ctggttgctt cttcttcatc aggccagacg tgacccgtgg caatcaccgc
     7381 taaatcaaac gtctcgctgg gtaaatcctg atttgtagcg agcatgacgc cagcatttgt
     7441 aatttgcaga tcggtaacct ggcatgattc ataaacagcc actgcaaatt tttgctgtcg
     7501 tgcctggtct actaatcgta aaaattgatc gcggaaatat tcgcccagca gaattcgcgg
     7561 taaaaactga cgatcgtgca aggtttcttt tttaacgcca taacgctgga gatggctggc
     7621 ttcttgcttt tgtagccatt cgagatacgt acaattaatc ggcggtattt caatactggc
     7681 aatatttgcc agcatcattt ttgagttttc ctcatcactg tatggcatcc cgacaccggc
     7741 ctcgtcagcc tgctcgaaga tagaaataga aagtggagtt tgttgctgta gaagcgagaa
     7801 taaggtgtag atccccgtag gcccggcacc cacaatagca atttttttca ttgttactta
     7861 cctgtacgca ttctttcgaa tttagtgtta tcagataagt ttggttgaga tttttagaat
     7921 ttgagaaatt tcagacaggg atgaaaaggg cgctaaccgc ccttgattaa attctgtcag
     7981 gcgagttttg cctgattgat ttgactaagt ggctcattga catcgaaaac tttggcgaca
     8041 acttcctcaa cgccgagatt tttcagtcgg gcagtatgtt tttccagata ggcaagggcg
     8101 cttttttcat cagtgaaaag gtagatccca ccggcttcgt ggttcttttc actttctgtc
     8161 cataccttcc acagaaaacc aggttcctga ttaatcgact cagcaagtgg tttaagctgc
     8221 tcagccattg cgtcgccaaa tgggccatta aaagcaaaat gaagttgtaa caaagttgcc
     8281 atagcgattc tccttctgat aacgcatacg aagacagtgt aaatctttta acgtattgga
     8341 tacagagaag aaacagaggg aaaaaggaaa agaccagatt aagaaaatct ggtgcgtccg
     8401 agtggactcg aaccaccgac ccccaccatg tcaaggtggt gctctaacca actgagctac
     8461 ggacgcagga tggtgcgttc aattggactc gaaccaacga cccccaccat gtcaaggtgg
     8521 tgctctaacc aactgagcta tgaacgcaac gttgtaggtg acaacgggga cgaatattag
     8581 cggcagagtg gaaaggtggc aagaggcaaa acgtaatttt ctgcgctatt tcgaccgttt
     8641 gcagagcttt taagcaaatt ggctatattt tgttgatttg caagggcgat ttttattcag
     8701 gatcgcattt acatctgata caacccaata attcgtgtat cagagtttaa acagagttgt
     8761 ttgccaccat ctcaaatctg tcgtggataa atcctccgta gcctttacac ttaggtagca
     8821 ataaaggcac acagatgcag caaaggaata aatcatggga tctgatgcga aaaacttgat
     8881 gagcgacggg aatgtgcaaa ttgttaagac cggcgaggtc cttggcgcga cgcaacttac
     8941 tgaaggcgag ttgattgttg aagctggcgg aagagccgaa aataccgtgg tcacgggggc
     9001 tggctggttg aaagtggcaa ccggtgggat cgccaaatgc acacagtacg gtaacaatgg
     9061 cacgctatcg gtcagcgacg gtgccattgc cacagatatt gttcagtccg agggaggcgc
     9121 aattagtctc tctacgctcg ctacggttaa tggccgccat cccgaaggtg aattcagcgt
     9181 tgatcagggt tatgcctgcg gtttgttgct ggaaaatggc ggtaacctgc gtgtactgga
     9241 aggacatcgc gcggaaaaaa tcattctcga tcaagagggc ggcctgttgg ttaatgggac
     9301 aacctcagcg gtcgtggtag atgaaggtgg tgaattgttg gtgtatccaa gtggagaagc
     9361 cagcaattgt gagattaatc agggcggcgt ttttatgctg gccgggaaag ccagtgatac
     9421 gttgcttgct ggtggcacca tgaataatct cggtggtgaa gactctgaca ctattgttga
     9481 gaatggatcc atttatcgtc tggggacgga tggccttcag ctctacagtt ccggtaagac
     9541 gcaaaacctg tcggtgaatg tgggggggcg ggctgaagtg catgccggta cgctggaaaa
     9601 tgcggtaata caaggtggaa cagtgatcct gttgtcaccc accagcgcgg acgaaaattt
     9661 tgtcgtagag gaagatcgcg caccggttga actgaccggg agtgttgcat tactggacgg
     9721 cgcttcaatg attattggtt atggcgcaga gctgcaacaa tcaacgatta ctgtacagca
     9781 gggcggtgtg ttgattctcg acggcagtac ggtaaaaggt gacagcgtca ctttcattgt
     9841 tggtaacatc aatctgaatg gcggaaaact gtggctgatc actggtgcgg caacgcatgt
     9901 gcaactgaaa gtgaaacgcc tgcgcggaga gggagcgatt tgcctgcaaa ccagtgcgaa
     9961 agaaatttca cctgacttca tcaatgtgaa aggggaagtt aacggggata tacgcgttga
    10021 gataacagat gccagtcggc aaactctgtg taacgctctg aaattacagc cagacgaaga
    10081 cgggattggc gcaacgctcc agcctgcgta aaaacacagg cgcaacccgt gaaggttgcg
    10141 ccttgtcttt attagcggga cgctcgttgc agaatgatgg ctgacggcat acgttgcagg
    10201 aaccgcatac gcagcatcat cataatggct gccgatgtca ggccgataat aaagcctatc
    10261 cagaagcctg ctggccccat aggttcaacg accagatcgg tcagtgccag aatatagccg
    10321 cttggcaagc ccagcaccca gtaagctgta aaggtgatat agaaaatgga acgcgtatct
    10381 ttataaccac gcaaaatccc actgccaatc acctggattg agtcagaaat ctgatatacc
    10441 gccgccagca acatcaaatg cgcagccagc gttacaacct cgggattgtc gttgtacaac
    10501 agggcgattt gctcccgcag tgaaaccgtg aaaatggccg tcagggttgc catacagaca
    10561 cccaccataa gcccggtccg cgcagcggtt tgcgcatcca gcgttgagcc ctgacccaga
    10621 cgataaccta cgcggatagt taccgctgcc gccagcgaca ttggaagcac aaacatcagt
    10681 gaactaaagt tcagggcaat ctggtgtcct gcgacatcaa caataccgag cggagacact
    10741 aacagagcca cgacggcaaa cagtgtcact tcaaagaaca gtgccagcgc aatcggcaaa
    10801 ccgagttgaa tcagtcgttt cataaccgca ggctcgggct tagcggtgcc tttttcgtta
    10861 cgaatatcgc gcatggagcg ggcgcgttta atgtaagaaa ccatggcaag gaacatgacc
    10921 caatacaccg ccgcagtagc cacgccacaa ccaacgccac cgagctcagg cataccgaaa
    10981 tgaccataaa taaagatata gttcaccggg atgttcacca gcaggccgat aaagcccatt
    11041 accatacccg gcttggtttt tgccagacct tcacattggt tacgggcaac ctggaagaac
    11101 agatatcccg gcgcgcccca caacaacgca cgcagataac ccacggcttt gtccgccaga
    11161 gccggatcga tgttttccat gtagcggata atgtaacctg cattccacag caccagcata
    11221 atgagaacgg aaacaaaacc tgccagccag aaaccttgtc gcacctgatg cgcaatgcgc
    11281 tcacgtcgac cggaaccatt taattgcgcg ataaccggcg ttaatgccag cagcagtccg
    11341 tgaccaaaga ggatcgccgg aagccagata gaagtaccga tagcgaccgc cgccatgtcg
    11401 gtggcactat agccgcccgc catcacggta tcgacaaaac ccatcgcagt ttgggcgatt
    11461 tgcgcgagaa tcaccgggat tgctaatgct aataacagac gcgcttcact gatatacttc
    11521 tgcacgtgaa caccttttat ttgtagttat atgagagact aaaaagccgc ctgaatgggc
    11581 agcaaaaaga agaagcaggg gaaattcagt ctattgtagc gaggatttat taattctcca
    11641 gtgaaaaaat agccattctt accccttcac tggtaagggc gttttccacc tgctattgtg
    11701 ctgaacagaa tgtcttaact gatttcagga gttgtaagta tgtttacggg gattgtacag
    11761 ggcaccgcaa aactggtgtc gattgacgag aaaccaaatt ttcgtacgca tgtggtggag
    11821 ttacccgacc acatgctgga cggcctggaa accggtgctt ccgtggcgca taacggttgc
    11881 tgcctgaccg tgacggaaat taacggcaac catgtcagtt ttgacctgat gaaagaaacg
    11941 ttacgcatta ccaatcttgg cgatttaaaa gtgggggatt gggtaaacgt tgagcgtgcg
    12001 gcgaaattca gtgatgaaat tggcggacac ttaatgtcag gtcatattat gaccaccgct
    12061 gaagtggcga aaatattaac ctcggaaaat aatcgccaga tctggtttaa agtccaggat
    12121 agtcagttga tgaaatatat cctgtacaaa ggatttattg gcatcgacgg tattagcctg
    12181 accgtcggcg aagtcacgcc aacgcgtttt tgcgtccatt taattccgga aacactggaa
    12241 cgcacgactc ttgggaagaa aaaacttggc gcacgcgtca acattgaaat cgatccacaa
    12301 actcaggcag tggtagatac ggtagaacgt gtgctggcgg cacgagaaaa tgccatgaat
    12361 caaccaggta cagaagcctg atctgaactc gcccccgaag agggggcgat agaatagcct
    12421 ttagcgagcg actcgaaggc cgttttcaac accgcgtgag aacaccacct gccagagctg
    12481 aatatcacgg gcgcggaaag cacctgcgca ggcattcaga taataggtaa acatccgttt
    12541 aaagcgttca ctgtagttat ccgcaatttc tggccatgcg gcgaggaatc gttcatacca
    12601 cgccatcaac gtagtatcgt aatcagcacc gaagttatgc cagtcttcca tcacaaagtg
    12661 gggttcgctg gattgagcaa tctggcgtac agagggcagg caaccgttcg gaaaaatata
    12721 tttattaatc cagggatcaa cattcagatc ggtttttttc gaaccgatag tatggagcag
    12781 gaatatacct tccggtttca aattacgatc caccaccgca aaataggtat cgtaattttt
    12841 cggtccgacg tgctcgaaca tccccacaga aacaatacga tcaaactggt cgttcaggtc
    12901 acgataatct tgcagcaaaa tggtgacatc caggccttta cagcgttcct gagccatttt
    12961 ttgctgttcg gcagaaatgg tgacgccgac cacgcttacg tcatagttag atgccatgta
    13021 gtgcgctagt ccgccccagc cgcagccaat atccagtacg cgcatccctg gttttaactg
    13081 caatttttca caaatcattt tgagcttcgc ctgctgggca gattccagat tatcggcatc
    13141 tttccagtaa gcgcaggaat attgcatgaa gggatcaagc atgcggctga acaagtcatt
    13201 acccaaatcg taatgctcct tgccgactat ccaggcacgt tttttactct gcagattgaa
    13261 gagacgagcg ccggcaatac gcagcgtgtc tttgaaatga tgggggagtt ggttctcgag
    13321 acctgcgcgt aagactttgc taaaaaacat atccagtcgg tcacattccc accagccatc
    13381 catataactt tcgcctaacc ccaaagagcc ttcctgcaga acgcgtttaa aaaaatcggg
    13441 gtttttcaca cgaatatccg ccggggcaga accgttaatg gctataccgg cacggctaag
    13501 taattcgttg gcgatacggt accagttgtc atccggtaca ctgacttctt ctatacacga
    13561 tgaactcata gtttctccat cactggtcgg tgatcagaac cttaaaacag cgtagacgct
    13621 ttttgggctt tgtgagaaat ctcgcagaaa aaaccgcgag tctataatcc gacgtagaac
    13681 agagggaaag gaggaaccct tgccgaaatg accatccata gtgagtcggg aacgatcctg
    13741 tccccgtaaa aacataatat ttatcgtagc tttaacaact cgaaattagt ataggccgct
    13801 aatttcaatc atacaacaaa attttgttag cagcctaagt ataagtatat cgatatagat
    13861 cagtgtgatt cgctatgagc gacttcggca ttgccatgat tcgggcagtc aacttcttca
    13921 caacgttgca tcatgtaacc cagcgcgacc agcactactg ttgataacat cacgctggtg
    13981 gtggtgagca atggcgtgct gatactaata agccaggaaa ctaccagact tgcgaggaag
    14041 cacagaccca gttgcagagt gttctgcaac gctgcggcgc gaccggttgc atgtgggaag
    14101 ggacgcagcg cctgggcgac aacaataggg tagatcgcgc cattggcaat cgccatcaca
    14161 cagaatggga tcaggatttc gaccagcgac acatggctaa tgaagcccgc agcccaggtc
    14221 gcaatgacgc tgacagcaaa cagcaccagc aaccacggta ataactgctt gccttgccat
    14281 ttctgcagtg cggcgcgaca gccataacca ccaatcagaa acgcgatagt ttgcgggaca
    14341 taacttaaac caataactgc cgggctgtag cccatttcac taaggatgaa cggtgaaccg
    14401 gtcagccatg caaaaaaact ggctgaacag gctgcgtata tcagcacgtt gccgcgatag
    14461 gttttagaac gtagcaggtc ggtaaaggtc agaccatcct gactattgtt acgggccttc
    14521 gtcgtgggtt tgagccagaa aataggcaga atcagcacca cggtaatggc aaacagggtg
    14581 gcgaaaatcg cctgccagga aaaatggacc agcagccagc ttcctaacag gggagccagt
    14641 gccggagaca gacccaccag cggcatgatg gtcgcaaaaa tacggttgac tttctgtgaa
    14701 ggataataat ctgtcactaa tgcttgccag ataaccgccg cggcgcagac ccccacagcc
    14761 tgtacaaaac gcaataccag cagcgtggcg gcgttttcta cccacagcat ccccagacta
    14821 cctaacgcaa aaattgtcag gccgattaat aacaccggtt tacgaccata acggtcggag
    14881 agtggccccc acagaagctg ggctgcggca aaaccggcaa ggaacagact aaggctggca
    14941 ctgacagcag acgcaggcgt ttgcaggtcg gcctgtatgg cggcgaaagc aggcagatac
    15001 atatcggttg ccagaaaacc gagtacgctc aaacccgcca gccagactaa aaatcttttc
    15061 ccaggttgca tgtatattct ctaaatctct caattaaaca ccgccgcaaa gtgtagggag
    15121 tgaaattagt gttgtgaaac ggtaatattt gatgactggt ttcaaaaatt ttgcaggcag
    15181 actatgtggt cagaatattc actcgaagtt gttgatgcgg tagcgcgtaa tggtagtttt
    15241 agcgctgcgg cacaggagct gcatcgtgtt ccttctgcgg tcagctatac cgtgcgtcag
    15301 ctggaagagt ggctggcggt accgctcttt gaacgacggc accgtgatgt ggagctgacc
    15361 gctgctggag cgtggtttct caaagaaggg cgctctgttg tcaaaaaaat gcagatcacc
    15421 cgccagcaat gtcagcagat agcgaacggc tggcgcggtc agttagctat cgcagtggat
    15481 aatattgtca ggccagaacg tacacggcag atgatcgttg atttttatcg ccattttgat
    15541 gatgtcgaac ttcttgtctt tcaggaagtg ttcaacggtg tctgggatgc gctttccgac
    15601 gggcgcgtgg aactggcaat tggcgcaaca cgcgcgattc cggtaggcgg tcgttatgcc
    15661 ttccgggata tggggatgct aagctggagt tgcgttgttg ccagtcacca cccgctggcg
    15721 ttgatggatg gcccgttcag cgatgatacg ttgcgcaact ggccgtcgtt ggtgcgcgaa
    15781 gacacctcgc gaacgctacc taaacgtatt acctggttgc tggataacca aaaaagactt
    15841 gtcgtacctg actgggaatc atcggcaacc tgtatctcgg caggattatg catagggatg
    15901 gtgccaacac atttcgccaa accgtggctt aatgagggga agtgggtagc acttgaactg
    15961 gaaaacccct tcccggattc ggcatgttgt ctgacatggc agcaaaatga tatgtcgcct
    16021 gcgttaacct ggctgcttga gtatctcagc gatagtgaga cgctgaataa ggagtggctg
    16081 cgggagccgg aagagactcc cgcaacgggt gattaacgac gatagtcgcg gaacgggccg
    16141 tcagccacgg agcggcgttc aatcaaacgc ggatgcactt caatggactg cggttcttca
    16201 cgtttgttga cgatacgatc caacagcatg ttgaacgctg tttcacccag cgaatctttt
    16261 ggctgatgga tcgtggtcag cgccggcgta aaatagcgcg cgttgcgcac gttatcataa
    16321 ccgatcagcg aaacatcctg cgggacgcgc aggcccattt catcagcagc acaaagtgcg
    16381 cccattgcca tgatatcgcc accacagaag acggcagtag ggcgatgcgg ctgcgacagg
    16441 atttgctgca tggcgcgata accggattca ggttcaaagt caccctgcac aatccagctt
    16501 tccggcacct tgatcatcgc ttcttccatc gccttcataa aaccggcaag gcggcctgcg
    16561 ccggtgttac gttccagcgg gccggggatg acgccgattt cgcggtgacc gcgttcaatc
    16621 agataacgcc cggccatgta gccgccttcg aacgcgttat caatgaccgc atcggtgaag
    16681 tcagcttttg cttcacccca gtccatcacc accattggga tatggcgata ctcttccagc
    16741 atcgccagca acggctctgg gtactcagaa cacatcacca gcagaccatc gacgcgtttt
    16801 tgcgccatca tcgacagata agcccgctgt ttctcaagat tgttccacgc attacccaga
    16861 atcagggtgt aacctttctg gaagcaattt ttttcaactg cttcaatgat ctcggcaaaa
    16921 taggccgctt cgctgctggt cgccagcaaa ccgatagact tggtgtggtt aaccttcagg
    16981 ctacgcgcca ccgcgctagg ggagtagtgt aattctttaa tcgctgccca cacggcgttg
    17041 cgcgtttctt cagcgacgaa acgtgttttg ttgatcacgt gtgacacagt tgtagtggaa
    17101 acgtttgctc gtttcgctac atcttttatt gttgccattc catttcactc cagaccctaa
    17161 cttcagctcc tgcaaaatcg caaggtaaac gtttgccttt acacacctta cgcacaacaa
    17221 aagtgttgcg gtacgccgga aaaacgacac ggagcgtcag gaaggggtca atggccagta
    17281 cgcaaatagt gtaagcgtgg aattttggcc gatcttgacg aaaaggggaa gtggtaaaaa
    17341 cacttatcat tataaatgac gggagatttt ttcatcacag tgtgtaaaaa tgcgtaatat
    17401 cactttttgt gtggtaataa aaaaggagaa aacttgatga gcaccgacct taaattttca
    17461 ctggtaacaa cggttatcgt cctcggtttg atcgtagccg tgggtttgac tgccgcgctg
    17521 cactgatttc ctctcgaggg agcgcgttct ccctcgcatc tccttcctgt ttgttagcaa
    17581 aatggcaaaa gtgttttctc tttttgttat tttcattttt ttgtgattgc tgtcatgctt
    17641 tcgccacttt aatgaatatc gcgtaatgac gcgaagaggt ggagataagg catgaaaatt
    17701 aactatccgt tgctggcgct ggcgattggc gcgtttggta tcgggacaac ggagttctcg
    17761 ccaatgggct tgttgcccgt cattgcgcgc ggtgtggatg tctcgattcc cgctgccgga
    17821 atgttaatca gtgcctatgc agttggcgta atggtgggcg cgccgctgat gacgcttcta
    17881 ctttctcatc gtgcccgccg cagtgcgttg attttcctga tggcaatttt cacgctaggc
    17941 aacgttcttt ccgccatcgc gccggattat atgaccctga tgctttcacg tattttgacc
    18001 agcctgaatc acggagcatt ttttggtttg ggttcagtcg tggccgcaag cgtggtgcca
    18061 aaacataaac aggccagcgc agttgccact atgtttatgg ggttaaccct ggcaaatatc
    18121 ggtggcgtgc cggcggcgac ctggttgggt gaaaccatcg gctggcggat gtcatttctg
    18181 gcaacggcgg ggctgggagt gatttcaatg gtaagtctgt tcttctcatt acctaaaggt
    18241 ggtacagggg cacgacctga agtgaaaaaa gagctggcgg tattaatgcg tccgcaggtg
    18301 ctgtctgcat tgctgacgac ggtactggga gctggtgcaa tgtttaccct ctacacctat
    18361 atctctccgg tactgcaaag tattacccac gcaacaccgg tgttcgtcac ggcaatgctg
    18421 gtgctgattg gtgtcggatt ctctatcggt aactatctcg gcggcaaact ggcagatcgt
    18481 tcagttaacg gcacgttgaa aggctttttg ttgctgctga tggtgattat gctggcaatc
    18541 ccgttcctgg cccgcaatga gttcggcgca gctattagca tggtggtgtg gggcgcagca
    18601 acctttgcgg ttgtacctcc gttacagatg cgcgtgatgc atgtcgccag tgaagcgcca
    18661 ggtctgtctt catcagtcaa tattggtgcc tttaatcttg gaaatgcgct gggagcagct
    18721 gctggtggtg cggtaatttc cgctgggctg ggatacagct ttgtgccggt gacgggggcg
    18781 attgtcgcgg gactggcatt attgctggtg tttatgtcag ccagaaaaca acctgaaaca
    18841 gtttgcgttg ctaacagcta attaaacaaa aacgcagaag gataaccttc tgcgttacag
    18901 ttgctgacaa cgtgcgattt atcttgccga atgcgctgtt aacgcctcat ccgcgttggc
    18961 atggtaagcg aagcgcatca ggcaatgctg catttgccat cagttattat gcagcgagat
    19021 ttttcgctac gaattcccag ttcaccagcg cccagaagtg ctccagatag ccaggacgtg
    19081 cattgcgata gtcgatgtaa taagcgtgtt cccagacatc aacggtcagc agcggagtcg
    19141 catcggtggt cagcggagta cccgcgttag aggttgaaac gatagccagt ttgccatcgc
    19201 tgtttttcac cagccaggtc cagccagaac caaagttttt gatcgctgca tcagtaaact
    19261 gcgctttgaa atcggcaaag ctgccaaaag atgcggcgat agcttcagcg acttttccag
    19321 tcggttcgcc accggcgttc ggtgccaggc agttccagta gaaagtatgg ttccagacct
    19381 gagctgcgtt gttgaatacg ccaccttcag agctgcgaat aatctcttcc agtgatttac
    19441 cttcaaacgc ggtaccttta atcaggttgt tcaggttagt gacataagtc tgatgatgct
    19501 tgccgtagtg atactcgata gtttccgcag aaatgtgcgg tgccagagca tctttagcat
    19561 atggtagtgc aggtaattcg aatgacattg ctactctcct ttattattaa tttgcatacg
    19621 tacaatagcc ttattgtgcg tatggatagg gtagcaaatt aaaaggtgag acaaaagatg
    19681 aacttaccct gttgccgtaa caacagggta aagttttagc gaagtgtttt tggggtcatt
    19741 acccgacgag cgccaacata gtggcgctgc cagtagtctt cactgagaga agtgatttga
    19801 atttcctgac ctgtgcgcgg tgactgaata aatttgccgt tgccgacata cacgccgaca
    19861 tgatcggctg taccgcgtcc ctgagtacgg aaaaagacca ggtcgccgtt tttcagctca
    19921 ctacgttcga taggtcctgc atcacgcagg tgatacattt cattcgccgt acgcggaata
    19981 cgaattttca ccaaatcttt ataagcgtaa taaaccaggc cgctgcaatc aaaaccggta
    20041 cgcggtgagc tgccgcccca acgatatggc ttaccaattt gctgcatcag tttattcatt
    20101 gccactttcg tggctttttg caccttcgct ttgtgcgcgt cggcaagggt tcctgacgcg
    20161 gcatttttga ctttcacaca atgcgattta tagcccttac gtttggtaca cttttcggta
    20221 aagctggctg aggccgtttt attgacggcg tgtttgctgc gagttttggc gtttttagaa
    20281 gcggtaatgg aagatttttt aacagaagaa gcggttttgc ttttggtcat acttttactg
    20341 gcggcttttt tcgctgtttt ctggctggct ttctgggtct ttttggtggt tgccgtgctt
    20401 ttctttttat ctgctttttt ggtgatatga gtggtagcag aactctccct ggcttgcttt
    20461 gaagcatggg ccataggcgt taaaggcagg gtggtaaaaa gtaaagcaca gagcgtgatc
    20521 gaaatacggt ttatccgcgc cactgagcgt ccctctgtta aggcaggtct atcatgcctg
    20581 cgtctgattt acaaaacttg atgattctaa tgcatagaag aagagaaagt taatagcttt
    20641 tattgttaaa aaatacgttt cgttggcgag cgcaaaaaaa tgcagcagtg aaagctaaaa
    20701 atggttataa atacgccagt ttgccgcgca tcagagaaga aacgaatatt tgaaatgcaa
    20761 caaatagagc aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg
    20821 cactgacccg ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg
    20881 agcaccacta tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg
    20941 aaaggttcac cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc
    21001 gcatgtggcg aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa
    21061 ctgccgaaat atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc
    21121 ggcggttgtg atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa
    21181 gaaactgccg ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac
    21241 tgacggatgc gaacgggcat ccgtcagtcc tttatccggt gcgccgtaaa acctcgtcct
    21301 tcagggcggg gatataaggc gcggttttcc acctgaccag gtgtttgctg ttgttcaatg
    21361 tactggcgga tgacggatat tggcgcacct tcgcagctac tggcaaaata gccaggactc
    21421 cacaaaacgc ctttgtagta atacctgact gcaatatctg gtcgatctcg tcgcagtaac
    21481 ctattcgata tgcctttgag gctgtttacc agactggata tcgccagttt gggaggatag
    21541 ttaattaaca aatggacgtg atctggttcg ccatccattt caaccagttc agctgcaaaa
    21601 tcagcacata catttgaaaa gtaagtgcgt agtttttctg tcgtgtcatg gtcaaaaatc
    21661 tggcgtcggt atttggtgac aaagaccagg tgaacatgca tcaggaaaac acaatgcctt
    21721 ccacgccgga tatcggtttc ttttttcaca gaccaaagta tcatttgacc tgtgaaacga
    21781 ttacaggcat ttaaattcca gttaagaccc ggtggtcaac aggagcgtga aatgaggcgc
    21841 ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg cgcttcagaa tgagaatcat
    21901 gaggccggta ataaatacat cccttacggg aaaatggctt cctggctggt tgagtggaaa
    21961 aacgccactg aaacgcaatg gcttaaagat tcgccctcac agccattgca acagtcactg
    22021 aaagaccttg agcgggctta caaaaacttc ttccagaagc gggctgcttt tccccgattc
    22081 aaaaagcggg gacagaatgc tgcattccgc tacccgcagg acgttaagct cgatcaggaa
    22141 aacagccgta tttttctgcc gaaacttggc tggatgcgct accggaatag ccgtcaggtc
    22201 acgggagttg tgaaaaatgt cactgtcagc cagtcctgcg gtacgtggta catcagtatt
    22261 cagacagaaa gtgaagtatc aactccggtt cacccttcag catcaatgat cgggctggat
    22321 gctggcgtgg ctaaactcgc cacgctgtca gatggcacag tctttgagcc tgtaaacagt
    22381 tttcaaaaaa accagaagac gctggcgaga cttcagcgac agttaagccg
//
LOCUS       DAJTPG010000061        22412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTPG010000061 DAJTPG010000000
VERSION     DAJTPG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            82..2682
                     /locus_tag="OO230_002467"
     CDS             82..2682
                     /locus_tag="OO230_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5692182.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="OO230_002468"
     CDS             2673..3656
                     /locus_tag="OO230_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5692183.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="OO230_002469"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="OO230_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="OO230_002470"
     CDS             4302..4817
                     /locus_tag="OO230_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5692184.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="OO230_002471"
     CDS             4783..5520
                     /locus_tag="OO230_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5692185.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="OO230_002472"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="OO230_002472"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5692186.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="OO230_002473"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="OO230_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5692187.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO230_002474"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="OO230_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5692188.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO230_002475"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="OO230_002475"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5692189.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="OO230_002476"
     CDS             10827..12734
                     /locus_tag="OO230_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5692190.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="OO230_002477"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="OO230_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5692191.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="OO230_002478"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="OO230_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5692192.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="OO230_002479"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="OO230_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5692193.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="OO230_002480"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="OO230_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5692194.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO230_002481"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="OO230_002481"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5692195.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="OO230_002482"
     CDS             complement(17402..19162)
                     /locus_tag="OO230_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5692196.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="OO230_002483"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="OO230_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5692197.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO230_002484"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="OO230_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5692198.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO230_002485"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="OO230_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5692199.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="OO230_002486"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="OO230_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5692200.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5258 c         5873 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ctaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAJTPG010000062        22282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTPG010000062 DAJTPG010000000
VERSION     DAJTPG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1029)
                     /locus_tag="OO230_002487"
     CDS             complement(<1..1029)
                     /locus_tag="OO230_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692201.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAK"
     gene            complement(1131..2111)
                     /locus_tag="OO230_002488"
     CDS             complement(1131..2111)
                     /locus_tag="OO230_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5692202.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2119..2829)
                     /locus_tag="OO230_002489"
     CDS             complement(2119..2829)
                     /locus_tag="OO230_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5692203.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3046..4869)
                     /gene="typA"
                     /locus_tag="OO230_002490"
     CDS             complement(3046..4869)
                     /gene="typA"
                     /locus_tag="OO230_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5692204.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5242..6651
                     /gene="glnA"
                     /locus_tag="OO230_002491"
     CDS             5242..6651
                     /gene="glnA"
                     /locus_tag="OO230_002491"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5692205.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6824..7873
                     /gene="glnL"
                     /locus_tag="OO230_002492"
     CDS             6824..7873
                     /gene="glnL"
                     /locus_tag="OO230_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5692206.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7885..9294
                     /gene="glnG"
                     /locus_tag="OO230_002493"
     CDS             7885..9294
                     /gene="glnG"
                     /locus_tag="OO230_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5692207.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9406..9516
                     /locus_tag="OO230_002494"
     CDS             9406..9516
                     /locus_tag="OO230_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5692208.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9745..11118)
                     /gene="hemN"
                     /locus_tag="OO230_002495"
     CDS             complement(9745..11118)
                     /gene="hemN"
                     /locus_tag="OO230_002495"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5692209.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11307..11816)
                     /gene="yihI"
                     /locus_tag="OO230_002496"
     CDS             complement(11307..11816)
                     /gene="yihI"
                     /locus_tag="OO230_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5692210.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12398..13030
                     /locus_tag="OO230_002497"
     CDS             12398..13030
                     /locus_tag="OO230_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5692211.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13412..16198)
                     /gene="polA"
                     /locus_tag="OO230_002498"
     CDS             complement(13412..16198)
                     /gene="polA"
                     /locus_tag="OO230_002498"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5692212.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16562..17494
                     /locus_tag="OO230_002499"
     CDS             16562..17494
                     /locus_tag="OO230_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5692213.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17535..18965)
                     /locus_tag="OO230_002500"
     CDS             complement(17535..18965)
                     /locus_tag="OO230_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5692214.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19120..19746)
                     /gene="dsbA"
                     /locus_tag="OO230_002501"
     CDS             complement(19120..19746)
                     /gene="dsbA"
                     /locus_tag="OO230_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5692215.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19763..20749)
                     /gene="srkA"
                     /locus_tag="OO230_002502"
     CDS             complement(19763..20749)
                     /gene="srkA"
                     /locus_tag="OO230_002502"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5692216.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20826..21095)
                     /locus_tag="OO230_002503"
     CDS             complement(20826..21095)
                     /locus_tag="OO230_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5692217.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21165..21749
                     /gene="mobA"
                     /locus_tag="OO230_002504"
     CDS             21165..21749
                     /gene="mobA"
                     /locus_tag="OO230_002504"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5692218.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21731..22258
                     /gene="mobB"
                     /locus_tag="OO230_002505"
     CDS             21731..22258
                     /gene="mobB"
                     /locus_tag="OO230_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5692219.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5513 a         5584 c         5333 g         5852 t
ORIGIN      
        1 ttttgcttcg ccgataggtg caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa
       61 gcccagcgta catgccatcc ctaaatagac cggcatactt tcgtgcggca gcataatcaa
      121 cagtacgagc gcagcggtac tgatgataaa ggtgtagcat agatatttac tcggcgattt
      181 caggatctta tctgaaatca tgccaccaat cggtccacca atcattttca gacagtattg
      241 gttaatgatg ccgtaagccc ccaccagcgc aacgggcaat agatagatgt ttttcaggaa
      301 tggaatgaag aatgtcaggc cacagtaaac cgcgtaaacg aagaagacgt tgaaagcgat
      361 aagccagatg gttttgtctc tcagcactga ggtcatactg gtgttttgcg atgctccgtc
      421 ttcttttttc acctcaacgg acggtgcctc ttctttgtca ttcaggacaa agaaaataat
      481 aatgcctacg gcaatcacaa taagggaata gaaccagatg cctgctttaa aacctaataa
      541 gccactgcca aaccaggtaa atactgccaa cgcagaaaat gccaccacgg tatcgacaat
      601 gccacgccct gtttcgaaga agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg
      661 acttaccgac ttgagcaaga ccggccagtt catcatgtcg caagtaacgc caaatagcgc
      721 ccagacaaag aggatgcccc aataccccgg cattgtcgtc aaataaacac ccagtaatcc
      781 tgtcgcaatg agtgagaatg acatggtgta tctgcgcggt agtttatcgg caaaataaat
      841 agacagaaaa aagcccactg tggtgacaaa tgagtttacc gacatagcat taccaatttg
      901 accattggtc aaatggaaat attcctgcat cgggatataa aacgcatctt tcagcgacgg
      961 taatttataa attgtaccgc cacacagtgt taatagacta aataacgccc atttcttttt
     1021 cgtgagcata acatccccta agcgattttt aaaatcactg ttttaaaggc aataagatac
     1081 cgaccattca tatggaatgg ttttattaac tgcgtatgag ttaaaactta ttagtggttc
     1141 agaagatggt ttgcctcttg tttgatttgt tgtagtacgt tacgcacatg atttatctga
     1201 ttaattgcat cttctttttc tttacgtaac cgcgcatcca gtaaatgatt ttctggtagg
     1261 ggtgtttcac tcatctggcg ataagggatc cacggattat catcttcgtc ttcaaagcgg
     1321 aacgccgacg catagtaatc aacctcttct tccaggccat atgccgtcac ctgcggctca
     1381 tcatcgccaa ggcagataag gtgcgttaat aacgctttga atggcatatc accctgacct
     1441 gaaatacagg ctttatgacc caggccaccc tgttctttaa cgatcaaggc atctttaata
     1501 tggacctggg taatatgcgg tgccatcgtt tttaaggcgt cgatgggatg ctcatttgca
     1561 ttgatcatgt tagcaaaatc aaacagtaag gaaagagact ccatctcact ttctttgacc
     1621 agcgacacca gctcatgact ttttaaatct tcatgctgct caatcgtaaa agtcaggccg
     1681 ctgtcctgat acgtttcccg tacataggca atgtcgttag cgataatcga taatacgtcg
     1741 cgcaaattac cttcataaca tggataaaaa cgtacggacg ttgccccagt tttcaacgcg
     1801 atggcgacgg cttcgtcaat agatgcctta tctgaagcgc tggtttcaat atgaatatca
     1861 aggttcagac ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca
     1921 agagaaaaac gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactt
     1981 gcgatatcaa gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca
     2041 taagcatgta aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt
     2101 attgtcacca taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata
     2161 ttcaatatct atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat
     2221 cgctggcata cccgcagaac cgccaagata cttgctgata tcgcccggca ttctgacggg
     2281 tttgaaaatc aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga
     2341 aaatgagtgg ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt
     2401 ttcgtgatag aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc
     2461 agttgatggc gtcactgcaa tcagtggcgc gagcgtctcg ggaatatcgc tggtacggtt
     2521 tttctcggta aacccccaag aaggctctcg cccttgctca attgcggccc gctgaaagct
     2581 cgccgacagc tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc
     2641 cttacggaag attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg
     2701 tttaatgccc agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt
     2761 gatgccatct ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt
     2821 ctccgccatt gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa
     2881 aaaagaaagc aattaacggt gtatgacttt gcagataatt gtgacaaagc tctctattga
     2941 cgtaatcaca gacttataag ggagatactc gcattggtgt gcgctcacga caggtaaaaa
     3001 aaaacctgcc atcgctggca ggttttttat gactaaaaaa cgaaattaat cgtctttcgg
     3061 tgcgcggttg gcgcggcgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga
     3121 ggtcggagtc acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt
     3181 catgcggata ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt
     3241 cagtttctta ccggtcaggc agtttacagt caggtcgtta gagcggctat gaataccgat
     3301 aatctgacct tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc gatcctgcag
     3361 accgaacagc gcgaacgcga cagctttacc ctgaccgtta gagatcagta caccgttctg
     3421 acgctgaccc acttcgcccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag
     3481 accagtaccg gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg
     3541 gatcacgtag tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc
     3601 tttacgttcg cccagcgcct gcatcacaga accctgatgc tgttcttcaa cgtccagcgt
     3661 cacgttttca tacggctctt gtttacgacc gtctatttca cggaagatta ctttcggacg
     3721 ggataccgcc agttcgaaac cttcacgacg catgttttcg ataagaacag agaggtgcag
     3781 ttcaccacga ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc
     3841 aacgttgtgt accagttctt tgttcagacg atcgaggatc tgacgagacg ttacgaactt
     3901 accttcttta ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc
     3961 atcaacggag agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat
     4021 gttcagttcg cccaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc
     4081 gatacgttcc agaccgaggt ggcccagcac tttaccgact ttcgcgttac gggttttgcc
     4141 ttcgctatcg atgatagtaa cctgctggtt cggcttcact ttaccacgct tgatgcggcc
     4201 aatgccgata acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg
     4261 accgtcaagg tcaacgtccg gcgcaggaac gtggtcaaca atcgcctgat acagcggggt
     4321 catgtcttcc gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta
     4381 aacgatcggg aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac
     4441 ctgatccaca acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat
     4501 cggcttcagg ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc
     4561 gtcaaatgcg tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc
     4621 accaccgaag tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt
     4681 gatagcggtg tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc
     4741 catcacgcgc tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt
     4801 gtctaccagg gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt
     4861 ttcgatcaca actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg
     4921 cactacaaaa caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga
     4981 tcgctttcac ggagcataaa aagggttatc caaaggtcat tgcaccaata tggtgcttaa
     5041 tgtttccatt gaagcactat attggtgcaa cattcacatc atggtgcagc ccttttgcac
     5101 gatgttaagc atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt
     5161 atctttttta cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgacaatga
     5221 ccaatccagg agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac
     5281 gaagtgaagt ttgttgattt gcgcttcacc gataccaaag gtaaagaaca acacgtcact
     5341 atccctgctc atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc
     5401 tcgattggcg gctggaaagg cattaacgaa tccgacatgg tgctgatgcc agacgcatcc
     5461 actgcagtga ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt
     5521 gaacctggca ccctgcaagg ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa
     5581 gattacctgc gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc
     5641 ttcctgttcg atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac
     5701 gatatcgaag gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt
     5761 ccggcagtga aaggcggtta cttcccggtt ccgccggtag actctgctca ggatattcgt
     5821 tctgaaatgt gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa
     5881 gtagcgactg ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct
     5941 gacgaaattc agatctacaa atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc
     6001 gcgaccttta tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg
     6061 tctctgtcta aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag
     6121 caggcgctgt actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca
     6181 aacccgacca ccaactctta taagcgtctg gtcccgggct acgaagcacc ggtaatgctg
     6241 gcttactctg cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa
     6301 gcacgtcgta tcgaagtacg tttcccagat ccggcagcta acccgtacct gtgctttgct
     6361 gccctgctga tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg
     6421 gacaaaaacc tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc
     6481 tctctggaag aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc
     6541 gtgttcacgg atgaagcgat cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc
     6601 gtgcgtatga ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag
     6661 ttgtactacc cggcgcacaa cgccgggatt tagttgccgt ggaaactttc agcccatctc
     6721 tgcatgggct tttttctccg tcaattctct gatgcttcgc gctttttatc cgtaaaaagc
     6781 tataatgcac taaaatggtg caacctgttc aggagactgc tttatggcaa caggcacgca
     6841 gcccgatgct gggcagatcc tcaactcgct gattaacagt attttgttaa ttgatgacaa
     6901 tctggcgatc cattacgcca accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa
     6961 attgtttggt acgccgttac cggaactgtt gagctacttc tcattaaata tcgagctgat
     7021 gcaagaaagt ctggaggcgg ggcaaggctt taccgataac gaagtgacgc tggtcatcga
     7081 tggtcgctcg catatccttt ctgtgacggc ccagcgtatg ccggacggca tgatcctgct
     7141 ggagatggca ccgatggata accagcgccg cttaagccag gaacaactac aacatgctca
     7201 acaagtagct gcccgtgatt tagtgcgcgg cctggcacat gagattaaaa atccgcttgg
     7261 cggtttacgt ggcgcggcgc agttactcag caaagcgtta cctgacccat cactactcga
     7321 atataccaaa gtgattatcg aacaggcgga ccggctgcga aatttggtcg accgtctgtt
     7381 ggggccgcag ctgcccggta cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt
     7441 ggtaacgctg gtgtcgatgg aactgccgga caacgtgcgg ttgattcgtg attacgatcc
     7501 cagcctgccg gaactggcgc acgacccgga tcaaattgaa caggtcttgc tgaatattgt
     7561 gcgcaatgcg ctacaggcgc tggggccgga aggcggtgaa atcattctgc gtacccgcac
     7621 cgcgtttcaa ctgaccttac acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga
     7681 agataacggg ccgggcattc cgcctcattt gcaggatacg ctgttttacc cgatggtcag
     7741 cggccgcgaa ggtggcaccg ggcttggctt atccatcgcc cgtaatttga ttgatcagca
     7801 ttcaggcaaa attgaattta ccagttggcc aggtcatacc gagttctcgg tttacctgcc
     7861 tatcaggaaa taaaggtgac gtttatgcaa cgagggatag tctgggtagt cgatgacgat
     7921 agttccatcc gttgggtgct tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca
     7981 tttgagaacg gcgcggaagt actggaggcg ctggcgagca aaacgccgga tgtgctgctt
     8041 tcagatatcc gtatgccggg aatggacggg ctggcgctgc tcaagcagat taaacagcgc
     8101 catccaatgc ttccggtcat cattatgacc gcacattccg atctggatgc tgccgtcagc
     8161 gcctatcaac aaggggcgtt tgattatctg cccaaaccgt ttgatatcga cgaagcagtg
     8221 gcgctggttg agcgcgctat cagtcattac caggaacagc agcagccgcg taatattcag
     8281 cttaacggcc caacgaccga tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt
     8341 attatcggtc ggctttcgcg ttcttctatt agcgtgctga ttaacggcga atccggcacc
     8401 ggtaaagaac tggtcgctca tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt
     8461 atcgcgctga atatggcagc tatcccaaaa gatttgatcg aatcagaact gtttggccac
     8521 gagaaaggcg cgtttactgg cgcgaatacc attcgtcagg ggcgttttga acaggccgat
     8581 ggcggcacgt tatttctcga cgaaattggt gatatgccgc tggatgtgca gacgcgtttg
     8641 ctgcgcgtgc tggcagacgg tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg
     8701 gatgtgcgga ttatcgctgc cactcaccag aatcttgaac agcgggtgca ggaaggtaag
     8761 ttccgtgagg atctgttcca ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc
     8821 gaacgtcggg aagatattcc ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa
     8881 ctgggcgtag aagcgaagtt gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg
     8941 tggccaggca acgtgcgcca gctggaaaac acctgccgct ggctaacggt gatggccgcc
     9001 gggcaggaag tgttgattca ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag
     9061 agtacttcgc aaatgcaacc ggacagttgg gcgacacttt tagcgcagtg ggcagacaga
     9121 gcgctgcgtt ccggtcatca aaatctgctt tccgaagcgc agccagagct ggagcggacg
     9181 ttactgacga ccgcgttgcg acatacgcag gggcataaac aggaagcggc gcggctactc
     9241 ggctggggcc gcaacactct gacgcgtaag ttaaaagagc tggggatgga gtgattcacg
     9301 gcttgtgtgt aaagattgat tattgagcgc aaattgctgg tattttgcgc tttactgttc
     9361 cgataagttc agtatgatct tgcccggaaa acggggagag tcattatgct ggaatcaata
     9421 attaatctgg tatcgagtgg cgcagttgac agccacacac cgcaaactgc cgttgctgcc
     9481 gtgctgtgtg ccgcgatgat tgggctgttt agctgaagct cgttgtttat gccggatgcg
     9541 gagaaagtcg taggcctgat aagacgcgac cagcgtcgca tcaggcatca tgctcagatg
     9601 cctgatgcgg cgtaaacgcc ttatccggcc tacaaaagca ataatttcaa caggttttgt
     9661 tgcccagcgc atcaggcaat cttgttctca gcacattgat ggcgctttcg ttttctactt
     9721 tgtaaacgaa gcgccattca ctctttaaat cacccgtgag aactgctgca tccgcgcttt
     9781 ctggcgcaga taggtatcaa agcacatgca aatgttgcgg atcagcaagc gacctttcgc
     9841 cgtcacctgt attcccttct catccacatc caccagccca tcttttgcta acggggcgag
     9901 cagcttgaga tcttccgcaa agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg
     9961 ggcgtaatcc agacggaagt tgcagatgag cgacttaatc acatcgcggc gaatacagtc
    10021 atcacgcgtt agcgcaatac cacgccacag cgcattgcct tgttcataca cttgctgata
    10081 gtactgcttc aactctttct ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga
    10141 aacgcccatc cccagcagat cggtatcgcc ctgagtggtg tagccctgga agttacgatg
    10201 cagcacgcct tcacgctggg ccaccgccag ctcgtcatca ggacgggcaa agtgatccat
    10261 accgataaac tgatagcccg attgcgtcag gaaggcgatg gtttcctgca ggatatcgag
    10321 tttttgctgc ggactcggca ggtcagcatc tttgattttg cgctgagcag caaaaatggt
    10381 cggcagatgc gcgtagttaa aaacgctcag acgatcgggg ttcagctccg ccacacgttt
    10441 cagggtaaag gcgaaactct ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt
    10501 ggtggaggta aaaccaatct cacgcgcatg gttaagcagt gcaaagatga actcttcatc
    10561 ctgctcgcgg ttaaccagac gttgcacttc tttgttgaag tcctgcacgc ccatgctcag
    10621 gcgattaaag ccttcggcgc gtaaatgatc gagtacatcc agttcgattt cccgcggatc
    10681 gacttcgatc gaaatctccg catcggtatt gaaatggaag ttttcgcgca gcagcttcat
    10741 cagacggctg atttgcgctt tattcagata cgtcggcgtt ccgccgcccc agtgcaattg
    10801 gctgacgtga cgcccggcga acagcggtgc acgatggacg atttcttgct ccagcgcgtc
    10861 cagatactga tcggccttgt gctgctggcg agtaacaatc ttattgcaac cgcagaagta
    10921 acaaagctta tggcagaacg ggatatgtac gtagagagat aatggacgct caggatagcg
    10981 cgccacggct tgtaaaaacg cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg
    11041 gtacgaggtg tatcgtggcc cggaatagtt atatttctgg atcagggcca gatcccagtc
    11101 gatttgctgt acagacatgc tcactccttc cgatggcgtc tctggcggct acggcgtacc
    11161 ggcggatgcc cgtttcgggt catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac
    11221 cgctgtagtt taacgaataa ccacaccaga taacatataa ccggaagggt tatcagcaga
    11281 acgggaaggc ccacggcggc aatccgttag ttgccccgca gcagacgcat catgtcttct
    11341 tgcttctcgt cttcttcctc ttcttcgtca tcgtcataag agaggccgag tttctgcatc
    11401 aactcatcaa tacgatccag tttagcatcc acccaggatt gctcttcggc gctcagggtt
    11461 tcgcctgctt ccagacgttc cagcagcgca tccagacgct catccgtttc cagtaattcc
    11521 aactccgcct gcggtgaaag cataggtttc tcactcttcg gtttgtgctg tttggtgact
    11581 ttttcagtca ctcccaatgg aataggggtt ttactgccaa tacgtggatc ttttggtgcg
    11641 ttctggcctt tgctgcctga cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga
    11701 ccacgacgtt ttttctgacg cttacggtca cgagcttcct gatccagctc ctcgcgtgtt
    11761 tttcgacgcg cttttgcatg acctttgctg cgtgagtttg aagatgatgg tttcataatg
    11821 gttcgtcttt tagccgtttt attcagtata gatttgcggc ggaatctaac agaaagcaag
    11881 caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata acccttaacg
    11941 ggtcttacaa tccttgcagg ccgccaacac accctgtttt tccattagcc ggaacaatcc
    12001 gtgttgattc catttccgtt taattacgtc tccggacgtt tgtcttcctg acaatcctct
    12061 gtcttcgcct cctggcgctc ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc
    12121 gctctatggg tgctacttta cgcctttgct ttaaacaaac aatcaaccgc catcagtatt
    12181 tgcacatttt cagagtttta tataaaagca attcactgat ttatatagct tatttgacat
    12241 gcatcgcaga attttctctg ctatttcgca taaagactat cggcaatgtc tcacaaagcg
    12301 tatggctttt acttacaacg ctcacgccga aaaatccgcc ttttattatg gttcaggtat
    12361 aatcgccaca aagctttgac tgacggagac gaccgctttg actaatttga attatcaaca
    12421 gacgcatttt gtgatgagtg cgcctgatat tcgccaccta ccttccgata ccggaattga
    12481 agtggctttt gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca cgctgactaa
    12541 ccagaaaagc ctggctcgta cctcaaaaac cccagggcgc acccagctta tcaacctgtt
    12601 tgaagtggct gacggcaagc gtctggttga cttgcctggg tacggttatg cggaagtccc
    12661 ggaagagatg aagcgcaaat ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag
    12721 cctgcaaggt ctggtggtgc taatggatat tcgccatccg ctgaaagatt tggatcagca
    12781 gatgattgag tgggcggtag acagcaatat cgccgttctg gtgctgctga ccaaagcgga
    12841 caaactggca agcggcgcac gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc
    12901 gtttaacggt gatgtgcagg ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa
    12961 gctgaggcag aaactggata cctggtttag cgagatgcag cctgtagaag aaacgcagga
    13021 cggcgaataa ttttcttgcc ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat
    13081 tcaatagatt acaattctac cgttggcctg ataaacatag cgcatcaggc attacggatc
    13141 ttttctttcg cccaataaaa aacgccccag tcattactga ctggggcggc taaaatattc
    13201 agccaaatcc gattacgtga agtaaaaggt ctgaaagata gaacatctta cctctgtacc
    13261 ctacgcgact aactttactc ttttttgttc agttcagaaa gcactttttg taattttttt
    13321 tcagttgttg cataggaatt tctaatagcc atcacaaaac gtgacagctt atgttgctta
    13381 cttacgaaaa aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac
    13441 tccccacttc caccagcaac ggcacatcca gacgggtaca gttttccatc agttgatgaa
    13501 tctgcttcgc gacggcatca acatcatctt tatgaacttc aaataccagt tcatcgtgta
    13561 cctgcatgat catacgtaca cgcggttgct cagcctgtaa ccacgcatca acggcaatca
    13621 tcgcccgttt gataatgtcg gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt
    13681 cagccgctgc acgacgagca ccattgctgg atttgatatc cggcagatac agacggcgtc
    13741 cgtccagcgt ttcaacgtag cccagctctt tcgcctgagc acgggtgcgt tccatatact
    13801 ccagcacgcc agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg
    13861 gaatgttcaa ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga ccaaagttga
    13921 tcgctttcgc gctacggcgt tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt
    13981 ctgccgccgt tgcccggtgg atatcttttc cttccgcgaa tgcggtcagc aagcctttat
    14041 cacgcgagag atgcgccata atgcgcagtt caatctgcga gtagtccgct gagacaatca
    14101 cataatcctc tggcgcaata aacgcctggc ggatacgacg accttcttcg ttacgcaccg
    14161 gaatgttttg caggttagga tcggttgacg ataaacgtcc cgttgcagtt actgcctggt
    14221 gataagaggt atgcacacgc ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt
    14281 aagtcgactt cagcttcgcc agaccacgat actccagaat cacttttggc aacggatagt
    14341 ccagcgccag ttcttccagt acctcttccg acgttgacgg cgcgccaccc ggcgttttct
    14401 tcagcggttt aataccctgt ttttcaaaca gaatggtttg caactgcttg gtggaagaaa
    14461 ggttaaattc ttcacctgca atttcatgcg ctttcttttc cagctcagcc agacgcaagg
    14521 tgagctcttc agaatgattg tgcagcactt tcggatcgat cttcacaccg ttacgttcaa
    14581 tgcgtgaaag caccggcacc agcggcattt cgatattctc gaagacgttc aacggccctt
    14641 tgtgtttctg cagatccggc cacattttca gatgcaactg caaggtgaca tctgcatctt
    14701 cggcggcgta acgtccggcc tcttcgaggg caatctggtt aaaggtcagt tgatttttgc
    14761 ctttacccgc aatctcttca aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc
    14821 tgtccatatc gtgacgcccg gcaacgctat tgagaatgta ggactccagc atggtatcaa
    14881 acgcaatccc acgcagttca atgccgtaat tcgccagaat accgcgatcg tatttcaggt
    14941 tttgcccgac cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac
    15001 gctcgtgaga gatttgatcg ggcgcatcaa gataatcatg agcaaccgga atatatgccg
    15061 ctacgcctgg ctcgatagca aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc
    15121 tgtcggtttc ggtatcaaat gcaaataccg gcgctttttc cagcttcgca atccacgctt
    15181 tcagtgtttc ttcatcaagg atagtgacgt agttgtcata agaaatcacc gttgccgtca
    15241 cttctggcgc ttcgtctgca acactggttt cctgcggctt cgcggctgtt tttgcccctt
    15301 tggcctgtaa ccatttgccc acttcgacat cagcagtcca gcgtttgaac tcatactttt
    15361 tgaacagccc caacaactct tccgctgccg gttgctgcac ttccagttgt tcacaggtca
    15421 gctccagttc aacatcggtt ttaatcgtcg ccaactggta tgagagataa gcaacttctt
    15481 tgttttgctc gagcttcgct gccattgttt tcgcgccacg gaagctcaac ccagcaattt
    15541 tttctggctc ggcatacagc gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg
    15601 ttttttcacc gacgcccggt acgccaggaa tgttatcaga ggagtcaccc atcagcgcca
    15661 ggaagtcgat gatcaattct ggcggcacgc cgtacttatt caccacctct tccggtccga
    15721 ggatggtatt cgtcatggta ttgataagcg taatatttgg cgtcaccagc tgcgccatat
    15781 ctttatcgcc agtgctaatc agcaccggac gcccggcttt ttcggcttcg cgcgccagag
    15841 taccgataac gtcgtccgct tctacgccag aaaccgccag cagcggcagt cccatcgctt
    15901 taaccatcgc gtgcagaggt tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat
    15961 gtgatttgta atgttcaaac agttcatcac gaaaggtttt tcccttggcg tcaaagacca
    16021 ccgctgcatg cgtcggttta tattgcatga tcagactgcg cagcatgttg aggacaccat
    16081 acatcgcacc ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc
    16141 gataaagata agatgaacca tctacaagga taagtggatt ttgggggatc tgaaccataa
    16201 tgtccgtgcc tgtttatcag attatgggta aaggatgcca cagacaggat gaaaacatga
    16261 gtttttcacc gcttttcgtg aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc
    16321 atgtggataa ctttgtgcat aaattattta catcgctttt aatttattat catgcttttt
    16381 acagcatgat gtttgtcttt gtttttcatg cagttaaaaa taaatccatc cctaataatt
    16441 gagataatgg gattttttct aatgtggata gatatgaatt atttttctcc ttaaggatca
    16501 tccgttattt gggtcatttt ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc
    16561 tatggctaac cttttgaata aattcattat gacgagaata ctcgctgcga taaccctttt
    16621 gctgagtatc gtattgacca ttttggtcac tattttttgt tccgtcccga tcatcattgc
    16681 cgggattgta aaactattgc tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg
    16741 tgattttatg atgtattgct ggtgtgaagg tctggcggta ttactgcatc tgaacccaca
    16801 cctgcaatgg gaagttcacg ggctggaagg gttaagtaag aagaactggt atctgcttat
    16861 ttgtaaccac cgtagttggg cagatattgt cgtactgtgc gtgctgtttc gtaagcacat
    16921 tccgatgaat aaatatttcc tcaagcaaca gctggcctgg gtgccatttc ttggcctggc
    16981 gtgctgggcg ctggatatgc cgtttatgaa gcgttattcc cgcgcttatt tattacgtca
    17041 tccggagcga cgtggtcaag atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct
    17101 gcatcccacc accattgtca atttcgttga aggctcccgt ttcacgcaag aaaaacatca
    17161 gcaaacccac tccacttttc aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc
    17221 gctaaatgta ctgggtaaac aattcgataa actgcttaat gttacgctgt gttatccgga
    17281 caataaccgt cagccgttct tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca
    17341 tgtggattta cagcctattg ccgatgagtt acatggcgac tacatcaatg acaaaagctt
    17401 taagcgccat tttcagcaat ggctgaactc attgtggcag gaaaaagacc ggcttctgac
    17461 gtctctcatg tcatcacagc gtcagaataa gtaagtttta ctcgcctctg aatgagcaga
    17521 ggcgagtgag tattttaatg aacaaaacgt ccggcacgag acataaattc ttcttcgctc
    17581 atctcctgtc cgttaaaaac aactttaccc ggtgtataac gcagctgcag cgaggcggta
    17641 ttgttgtcaa tcgtgattaa ctgaaacatc tgacctaatg tcatcatccc gctgatttgt
    17701 ttatcagcct gcttttgcgc tttttccgca tccattcctt cagataaatt aagctgtttt
    17761 gcggtttctg tgacgacatt aagcggtaac ttcacatcaa aattgagcga tttgatatct
    17821 ttgtttgtgg atgatgaaga tttggctggg tcggcaatac tgatatccag attggcattt
    17881 agttcgccga gtgcgttctt ccatcttacc ggttgtttaa tggtcggctc acttttttgt
    17941 aataacggta aatactcttt gaacaatgca gcattcactt cttgcagagc aacttcatcg
    18001 tttgctaact cagggtgtgc agcaagctgc ttctgcatcg caatgttata ctggatgata
    18061 aattggcgca ctgctgaggg atcaatagat tcagcaatca atgaccactc accactaccg
    18121 agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac attgatataa
    18181 tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat ttgggcaacg
    18241 tcttccccgt gattgataat gttaatctga ttaaatttcg actcgctttc accaaccgga
    18301 aatttctttt cttctaatcg cgccagagaa tcaatggtaa aactttttgc cgttactttg
    18361 ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc caggttagac
    18421 aatgaactac catcaccatt aaaagtaaac tggccctcac caaaagaaaa ttttccatac
    18481 tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc
    18541 gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag ttcattattc
    18601 actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat taacattgtg
    18661 atgggcaacg gaccgtgctc aacatccaca tcaaatacaa ctttctggcc tggcttgata
    18721 ttgagatcgg gtgcaccgtt atcgaatgtc atttgcattt gaaaacggga attaaaaaag
    18781 cctttgtcaa agtttttata acttaacgtc atatcgtagg catgttcacc tttctttttc
    18841 gcatcgttaa aatctttaat aaacttttcg ataccaggct gaatttgcgt acctgtgtac
    18901 catgtgccac caccccagat tacggctaac gcaacaataa cacctgtagc tgacttacgt
    18961 atcatataaa tattccctga taaaacagca aaactttgaa tatccactta tgctgaaata
    19021 atgtccagcg gcaggatgca ttatcagaat gtatttatat ttattaaata ataaaaaaag
    19081 cccgtgaata ttcacgggct ttatgtaatt tacattgaat tatttttcct cggacagata
    19141 tttcaccgta tcagcatact gctgaacaaa aacatccata ttgctggtat ccataccctg
    19201 cggattcagc tgatatttac cgttaacaaa catcgccgga acaccacgca attgcacatc
    19261 agctgcggct ttttcctgct gagcgaccag agatttcacc acgaagctgt tccacgccgc
    19321 gtcgtactct tcacctttaa tacctgcatt gataaataca tcgcggatat cagatgctga
    19381 acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt tgtcttccac
    19441 gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt caccacccat
    19501 gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct tcacattatc
    19561 agaaatatgc agaacttctt caaactgata gcagtgcggg cagaagaaag agaaaaactc
    19621 cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt taccatcttc
    19681 atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca gccaaatctt
    19741 tttcatgatc tactctctcc gattaataca taggtgttaa ttgcaaaggg ggttcttgta
    19801 gaacttttgc ctgttctata aaagtcgccg tctgtcgtag ccagtaatct tccccggtta
    19861 accacgggaa atttttcggg aacgcgggat cagcccaatg ccgcattagc caggcaagat
    19921 aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact
    19981 cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc tgggctttat
    20041 cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca tcatccagat
    20101 caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct ccatgtagcc
    20161 gcagaacggt gaaatcttcc cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg
    20221 ctttcagaaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca aacagcttgc
    20281 gaggctcaat gagatattca ttcagaccga tggtcgggcg atgaataaaa agctgtttgc
    20341 gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga tcgatattat
    20401 cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat
    20461 tcaataaagt ctgaccgtta aaggccacag gtgctgcgac cggaacttca tcatttacca
    20521 gctgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt tcagggcgat
    20581 aaaatttgac gacaaaacgt cgacgctctt cgtcctgaaa ttgatagaca cggttttcat
    20641 agctgttaag cggggtaaga ccggaatcca cccggatccc ttgcttaaac agagcgtcca
    20701 tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc ccatcatccg
    20761 gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc gccgcttaca
    20821 agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca
    20881 taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat tttcaggtga
    20941 tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga ctctttcgcc
    21001 agtttctgca aaaattgcaa caagttaaag tctggctctt tttgccaggc tggctggagg
    21061 agttcaataa cttcattcag acgtttacat ttcatagtaa tgctccttac tcttgagaca
    21121 gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat ctaatgacga
    21181 cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggcggc gtagataaag
    21241 gattgctgga cttaaatggc aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc
    21301 agctctctca cgtcgtggtt aatgctaatc gtcatcagga aatctatcag gcaggcggtc
    21361 tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcagga atgctttcag
    21421 taatgcagca ggaagctggt gagtggtttt tgttttgtcc gtgcgatacg ccttacattc
    21481 cccatgattt agccgcccgg cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc
    21541 atgacggtga acgcgatcac ccgactattg ctctggtaaa ccgcgctatt gagcctttat
    21601 tactggaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt ctggctggcg
    21661 gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat acgccagagg
    21721 agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg cgtggagtgg
    21781 taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatgcgcca gagggatccg
    21841 tccggggctg attaagcata cgcaccatga tatggatgtt gataagccag gcaaagatag
    21901 ctatgagctg cgcaaggctg gcgcggtaca aaccatcgtt gccagccagc aacgatgggc
    21961 cttgatgacg gaaacaccag acgaagaaga gctggatcta cattttctcg caagtcgaat
    22021 ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag agatcgcaaa
    22081 gattgtgctg tttcgcgatg gagccggaca tcgacctgaa gaattagtga tagacaggca
    22141 tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac tggatattaa
    22201 tgatgttgag gggctggctg attttgtggt ggagtggatg caaaagcaga acggataagc
    22261 aagagtaggc tggatcagac gc
//
LOCUS       DAJTPG010000063        21519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTPG010000063 DAJTPG010000000
VERSION     DAJTPG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO230_002506"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO230_002506"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5692220.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO230_002507"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO230_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5692221.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO230_002508"
     CDS             complement(2649..3200)
                     /locus_tag="OO230_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5692222.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO230_002509"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO230_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5692223.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO230_002510"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO230_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO230_002511"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO230_002511"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5692224.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO230_002512"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO230_002512"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5692225.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO230_002513"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO230_002513"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5692226.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO230_002514"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO230_002514"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5692227.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO230_002515"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO230_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5692228.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO230_002516"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO230_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5692229.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO230_002517"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO230_002517"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5692230.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO230_002518"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO230_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5692231.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO230_002519"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO230_002519"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5692232.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO230_002520"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO230_002520"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5692233.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO230_002521"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO230_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5692234.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO230_002522"
     CDS             complement(18170..18565)
                     /locus_tag="OO230_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5692235.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO230_002523"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO230_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5692236.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO230_002524"
     CDS             19481..20074
                     /locus_tag="OO230_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5692237.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO230_002525"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO230_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5692238.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21519
                     /locus_tag="OO230_002526"
     CDS             20794..>21519
                     /locus_tag="OO230_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5692239.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLE"
BASE COUNT         5196 a         5325 c         5671 g         5327 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaatga ttcgcaggag
    21421 ttaaataatt cacagaagtt aaataattcg caggagttaa ataactcgca ggtgagttgt
    21481 aaaggttcag ttgattctac gattacggat ttattagaa
//
LOCUS       DAJTPG010000064        21489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTPG010000064 DAJTPG010000000
VERSION     DAJTPG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(26..2095)
                     /gene="glyS"
                     /locus_tag="OO230_002527"
     CDS             complement(26..2095)
                     /gene="glyS"
                     /locus_tag="OO230_002527"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5692240.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2105..3016)
                     /gene="glyQ"
                     /locus_tag="OO230_002528"
     CDS             complement(2105..3016)
                     /gene="glyQ"
                     /locus_tag="OO230_002528"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5692241.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3111..3410)
                     /locus_tag="OO230_002529"
     CDS             complement(3111..3410)
                     /locus_tag="OO230_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5692242.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3585..4580
                     /gene="wecH"
                     /locus_tag="OO230_002530"
     CDS             3585..4580
                     /gene="wecH"
                     /locus_tag="OO230_002530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5692243.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4622..5059)
                     /locus_tag="OO230_002531"
     CDS             complement(4622..5059)
                     /locus_tag="OO230_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5692244.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5105..5446)
                     /locus_tag="OO230_002532"
     CDS             complement(5105..5446)
                     /locus_tag="OO230_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692245.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5615..7069)
                     /gene="xylB"
                     /locus_tag="OO230_002533"
     CDS             complement(5615..7069)
                     /gene="xylB"
                     /locus_tag="OO230_002533"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5692246.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7141..8463)
                     /gene="xylA"
                     /locus_tag="OO230_002534"
                     /pseudo
     CDS             complement(7141..8463)
                     /gene="xylA"
                     /locus_tag="OO230_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8829..9821
                     /gene="xylF"
                     /locus_tag="OO230_002535"
     CDS             8829..9821
                     /gene="xylF"
                     /locus_tag="OO230_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5692247.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9899..11440
                     /gene="xylG"
                     /locus_tag="OO230_002536"
     CDS             9899..11440
                     /gene="xylG"
                     /locus_tag="OO230_002536"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5692248.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11418..12599
                     /gene="xylH"
                     /locus_tag="OO230_002537"
     CDS             11418..12599
                     /gene="xylH"
                     /locus_tag="OO230_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5692249.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12677..13207
                     /locus_tag="OO230_002538"
     CDS             12677..13207
                     /locus_tag="OO230_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5692250.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13319..13855
                     /locus_tag="OO230_002539"
     CDS             13319..13855
                     /locus_tag="OO230_002539"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5692251.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13963..14787)
                     /locus_tag="OO230_002540"
     CDS             complement(13963..14787)
                     /locus_tag="OO230_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5692252.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15107..17136
                     /gene="malS"
                     /locus_tag="OO230_002541"
                     /pseudo
     CDS             15107..17136
                     /gene="malS"
                     /locus_tag="OO230_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17314..18567
                     /gene="avtA"
                     /locus_tag="OO230_002542"
     CDS             17314..18567
                     /gene="avtA"
                     /locus_tag="OO230_002542"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5692253.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18718..19191)
                     /locus_tag="OO230_002543"
     CDS             complement(18718..19191)
                     /locus_tag="OO230_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5692254.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19293..20141)
                     /gene="yiaJ"
                     /locus_tag="OO230_002544"
     CDS             complement(19293..20141)
                     /gene="yiaJ"
                     /locus_tag="OO230_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5692255.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20341..21333
                     /gene="yiaK"
                     /locus_tag="OO230_002545"
     CDS             20341..21333
                     /gene="yiaK"
                     /locus_tag="OO230_002545"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5692256.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21345..>21489
                     /locus_tag="OO230_002546"
     CDS             21345..>21489
                     /locus_tag="OO230_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5692257.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5401 a         5485 c         5274 g         5329 t
ORIGIN      
        1 ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca acgcgcagga
       61 acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct ttgtcatcaa
      121 ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct gccagttcga
      181 ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca cgtagcacca
      241 caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca ttcacgcgat
      301 cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc gccgccgcca
      361 gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg gcatcgaaat
      421 cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg gtataacctt
      481 cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca tcaactacgt
      541 tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc gtttgcagat
      601 caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca cgacgcagcg
      661 caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc gccagagtat
      721 ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg tcatcaccgg
      781 caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct tcgccatcgt
      841 gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag accatgttgg
      901 tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg tcagcgccaa
      961 tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca cgcagcgtac
     1021 ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc agacgttttt
     1081 tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg actttctcgt
     1141 taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag ataaagttcg
     1201 gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct ttcatggtgt
     1261 aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg gtcagaacga
     1321 ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag tcagcgttac
     1381 cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta cgttcttcgt
     1441 aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg gcgttgtcga
     1501 tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg gactgaatgc
     1561 ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg tgcaccggac
     1621 gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc agtttcgcca
     1681 gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc acatgggcgc
     1741 gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca acggtaatac
     1801 cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg tcgaacgcct
     1861 gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc gccaggttag
     1921 ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg tgtgcgaggc
     1981 ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg ctgcgcagtg
     2041 cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca gacatagccg
     2101 cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa gcgtaatatg
     2161 cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga cgctcggtga
     2221 cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc agaatacgct
     2281 cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct tctttctcgt
     2341 actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg taagtggact
     2401 gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc gggccgtcgc
     2461 tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga cgttccagac
     2521 cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc tggaagtaag
     2581 tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc caggccccca
     2641 gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc gggtccatgc
     2701 ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat ggcttaatga
     2761 ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag cgaccatcgg
     2821 tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc agcgcgcgca
     2881 gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt tgaacaatgg
     2941 tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg aaggtcctgg
     3001 tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg aagggatcag
     3061 tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc ttaacgcatg
     3121 gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc tttacggaag
     3181 gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac gtcgattttt
     3241 tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc catattcaac
     3301 gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg tgtagaacaa
     3361 cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat taaattaccg
     3421 tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat cttacgaatt
     3481 tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc gccaaccaaa
     3541 tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag cccaaaattt
     3601 actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt cacaccacta
     3661 cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc gccaatgttc
     3721 tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc tatctctttt
     3781 ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt ctgatttttt
     3841 atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg gagttagcgc
     3901 tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc gcgattgcgg
     3961 tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa atgttgctgg
     4021 tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag aaaattgacg
     4081 gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac tacattctgt
     4141 atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca ctgtcgtggg
     4201 tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca ttatatgaat
     4261 tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg atggttttta
     4321 tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt accattcgcg
     4381 gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc ttgattatcc
     4441 atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat attatttgga
     4501 tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa cgaatcgaca
     4561 gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg gcttacgtga
     4621 attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt attccggcat
     4681 cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa gccagtccat
     4741 aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc agtagagcaa
     4801 cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc ggtatgcctt
     4861 catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct aacaccagta
     4921 cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc cctaacaggt
     4981 aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc ggtgaatagg
     5041 ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa gagctttatg
     5101 ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa aaggcgatgg
     5161 ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata aacaagagac
     5221 cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc agttgcccga
     5281 gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac acgccaagaa
     5341 aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga taaaccagcg
     5401 ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg atctccatat
     5461 ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg ccattaacaa
     5521 atgattttca gaataaattc atactaaata ttaattaatt agtgagatat atagatgtga
     5581 attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc agaagttgct
     5641 gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc tgcgcatctg
     5701 gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat ttctctggat
     5761 tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc ccccccgtac
     5821 ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac tcactacgcg
     5881 ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc acgacatcca
     5941 tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt tcattggggc
     6001 catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg tgtggtgtac
     6061 gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca gcctgttgag
     6121 ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc caatccagac
     6181 acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc gcatggcaaa
     6241 agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca aaatagaccc
     6301 ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca ccaactgcac
     6361 cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc cacgctttcg
     6421 caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg ggcatctggt
     6481 cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt gcgacatcca
     6541 gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc atacgcagac
     6601 gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc ggctcatgcc
     6661 gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg ccggtaatca
     6721 ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag cgcccgtcgt
     6781 tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt gctccatgca
     6841 tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg cccagagctt
     6901 tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac caaagtggat
     6961 gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc tgctcgttga
     7021 gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata tcgatcgttc
     7081 cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca cagttagccg
     7141 ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc gaccactctg
     7201 atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca gtgacatttg
     7261 gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct gcgcgatgcg
     7321 tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca gtgccatcgt
     7381 atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt gacgacgtac
     7441 tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa tttcatacat
     7501 caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc ccagttgcgc
     7561 atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg tggctatttc
     7621 atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca gtttaatctc
     7681 tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt aatcatattg
     7741 atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga agccgatttt
     7801 atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct cctgacgcaa
     7861 gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat agttttcacc
     7921 gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc agctgaagac
     7981 ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa agcagttagc
     8041 ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga catcaaccat
     8101 ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa catccacatc
     8161 gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga catctgcttt
     8221 acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa acgcccccac
     8281 accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg caaaacgcaa
     8341 gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac ggaatgctaa
     8401 cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa aataggcttg
     8461 catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt aatattgggc
     8521 actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa ttcacaagtg
     8581 tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg aagataaaaa
     8641 tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa gaaataaacc
     8701 aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag ttgctaaaaa
     8761 ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac tacagaaggc
     8821 cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt ctgcttacca
     8881 acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat ctccgtcttg
     8941 aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc ggtgcgaaag
     9001 tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt gaaaacatga
     9061 ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta ttaagtaacg
     9121 ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt atgattaacg
     9181 atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa ctgcaggcaa
     9241 aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc tcgccggtag
     9301 ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct tacgttgatt
     9361 ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg gaaaacgcat
     9421 tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct gtagttgcct
     9481 caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt ttatcaggga
     9541 aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt gctgccggta
     9601 cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc gcagaaattg
     9661 ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat aatggcctga
     9721 aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac atcaaagata
     9781 cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc cccggcgcgg
     9841 agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg gagtcgttat
     9901 gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga aggcgattga
     9961 taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg aaaatgggtc
    10021 tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct cctacgaagg
    10081 cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata ccgaacgcaa
    10141 aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg tgctggaaaa
    10201 tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc tgatgacgct
    10261 acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata cccgcgttgg
    10321 cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta ataaacaggt
    10381 gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa cgtcggtttt
    10441 actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata tttcgcacaa
    10501 actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg gacagcacat
    10561 tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga tggtcgagcg
    10621 agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa tattacgtat
    10681 tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta atgatgtctc
    10741 gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg ccggacgtac
    10801 cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa aaatttatat
    10861 tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg ggattgcgat
    10921 ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg gtaaaaatat
    10981 taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg acgcggcaga
    11041 gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt cccccgacct
    11101 tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc gctgtctgtt
    11161 acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata ttggcgcgaa
    11221 atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg ttattgtcat
    11281 ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga tgcatgaagg
    11341 gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga tggaagccgc
    11401 attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg gccgtaccga
    11461 catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg atgattgcag
    11521 ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac ttaagcgccc
    11581 gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg gtaggaatgg
    11641 tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg gggctattag
    11701 gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt accatcattg
    11761 tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc gcgtaccgca
    11821 aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc atactcattg
    11881 gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa attgggcaaa
    11941 gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg gcttttgttg
    12001 gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct ccggcctcta
    12061 ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca atctggctgt
    12121 tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg ttactcggcg
    12181 gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc ggcggcaatc
    12241 tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc gtgttcgcga
    12301 ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt ggcgctggtt
    12361 caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg attggcggca
    12421 ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca tttatcatgg
    12481 cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag tatatcgtta
    12541 aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc cgttcttgat
    12601 tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt ttgcatgatt
    12661 cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt tcaatgccaa
    12721 taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg catcacaatc
    12781 agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa tcaaggactg
    12841 gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag cgctggctga
    12901 tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa gttacccacc
    12961 cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt tgcatttaaa
    13021 agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg gcaaacgttg
    13081 ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt atcgcggagt
    13141 ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa atcggctggc
    13201 agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg acgcccgagc
    13261 gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa aattatgcgt
    13321 gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc tttcttcggt
    13381 cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc gattattaga
    13441 taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca ttgaacggcg
    13501 ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc attacattcg
    13561 taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg ggatctcgcg
    13621 ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg caatgattca
    13681 tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt cgatcaatga
    13741 gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt ttaaaaaagc
    13801 atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt tgtagcgatg
    13861 atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc gagcaaaagc
    13921 cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat gcgccgcgat
    13981 taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct tccctttggt
    14041 cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat ccgctttacg
    14101 cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca ggttagtgac
    14161 ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca ctttacccgg
    14221 cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat tgttgcgcgc
    14281 cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca tactggtagg
    14341 gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat taccagacca
    14401 cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag gtgcccattg
    14461 gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg cattttggct
    14521 ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc cggaagggta
    14581 ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact cttgtttact
    14641 gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg ttgttgtgtg
    14701 cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa tcatcgcccc
    14761 atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat cattccaaca
    14821 ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa tagctgtcaa
    14881 gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga ttcagattgg
    14941 cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt gcgcgcgatc
    15001 gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt gtctggctta
    15061 tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga aactcgccgc
    15121 ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt ctccggggtt
    15181 ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt tgcccaaagg
    15241 tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag atgcgataaa
    15301 actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat ggcgattgtt
    15361 cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa cattgatgat
    15421 ttccatccag aacgcgccga accggtagca aacctggtcc gtgaatgccc gaaatgggat
    15481 ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc cgtacgggat
    15541 tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca acccgctgct
    15601 accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc ccctttcgac
    15661 tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg cgatcccagt
    15721 aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac ttttcacggc
    15781 ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg cgttaatgct
    15841 ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggtgg tacaaaaggc
    15901 gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa tcttgatgcc
    15961 aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca gcgcggtatt
    16021 cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc ggatatgcag
    16081 gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac gctgggtgaa
    16141 cgctggagcg actggaaacc tgccgccgga caaacctggc atagctttaa cgattacatt
    16201 aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat cagaacggat
    16261 atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc ctttttgccg
    16321 gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa aaacaaaacg
    16381 gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac ccactggtta
    16441 agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc caaacatgtt
    16501 gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg cgaatggaaa
    16561 aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg tgaagcctgg
    16621 ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat gatcaatttc
    16681 gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga tacgacctgg
    16741 cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc gtcgcatgat
    16801 acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt agcgccaggc
    16861 gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac aggttctgat
    16921 ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa atctgccgcc
    16981 aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc aattggcgcg
    17041 ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga gcatggcgac
    17101 gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc cgttcgtcag
    17161 tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt gcgggataaa
    17221 gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt tattgttacc
    17281 ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt tggtgacaaa
    17341 tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg tttacgcacg
    17401 cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat gcaggactac
    17461 ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc actgtgtaac
    17521 tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat gctgcgcgag
    17581 aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag ccagagcgcg
    17641 tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt caaaaaagtg
    17701 ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga agaagatctg
    17761 tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa ataccacgtc
    17821 gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc ccggccgacg
    17881 aatccaacag gtaatgtgat tactgacgaa gagttgctga agcttgacgc gctggcgaat
    17941 caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc gggtatcatc
    18001 ttcagtgaag ggcgcccgct atggaatccg aatatcgtac tgtgcatgag tctttccaag
    18061 ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat catcactgcc
    18121 atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc ggcgatgatg
    18181 tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat caaaccgttt
    18241 tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc ggaaaatcgc
    18301 tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa ggatttgccc
    18361 attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat ggtgccgggg
    18421 cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg tatgcgcatg
    18481 aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc ggaagagata
    18541 gaaagagcct gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt atcaggccta
    18601 catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag gcgtttacgc
    18661 cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga aaacgtttca
    18721 aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc actgcaacgc
    18781 ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc acagatcgca
    18841 cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa acgggcaagc
    18901 cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat gcccatgttc
    18961 gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac actgatgaca
    19021 ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa taaactcgtc
    19081 tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag ccacttcaca
    19141 ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca tgatcattcc
    19201 tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat ccaacgaagg
    19261 aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgcccag gtcatccctg
    19321 acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg tttcaggaga
    19381 tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac ggcgtacggc
    19441 acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag ttcgttttct
    19501 tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa catcgcgggc
    19561 agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt ttcccagtat
    19621 gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc ggaacagtag
    19681 agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt gggttccagc
    19741 ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt ttcaccagtg
    19801 gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag cgaagacagc
    19861 gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc ggcgggcgcg
    19921 gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt actcttattt
    19981 aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt gctcaaaatc
    20041 tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc tttttcctgc
    20101 gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg tcctgatggt
    20161 agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac ttgattttta
    20221 tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata tatttgaaat
    20281 ctagttccgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag gaagcctagg
    20341 atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat ttcacgcggc
    20401 gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac cgaatccggc
    20461 gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga aaacggcgat
    20521 atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga acagtgggat
    20581 gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc cattgaactg
    20641 gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg gatgcgcggc
    20701 ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg gaccaactcc
    20761 atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa cccgctgatc
    20821 gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgttctc ttacggcatg
    20881 ttagaagtta accgcctggc aggccgtcag ctcccggtcg atggtggctt tgatgatgag
    20941 ggcaatttga ccaaagaacc tggcgttatc gagaagaatc gccgcatttt gccgatgggc
    21001 tactggaaag gttctggcat gtcgattgtg ctggatatga tcgctactct cctttccgac
    21061 ggcgcatcgg ttgccgaagt cacccaggac aacagcgacg aatacggcat ttcacaaatt
    21121 tttattgcca ttgaagtgga caagcttatc gacggtccca cccgcgatgc caagctgcaa
    21181 cgtatcatgg attacgttac taccgctgaa cgcgctgacg aaaaccaggc catccgctta
    21241 cccggccacg aatttactac cctgctggcc gaaaaccgcc gtaacggcat cactgttgat
    21301 gacagcgtgt gggccaaaat ccaggcgtta tgaggagata agtcatgatt tttggacata
    21361 tcgcgcagcc caatccgtgc cgtttgcccg ccgccattga aaaggcgctc gattttctgc
    21421 gcgctaccaa cttcaacgtc ctggagccgg gcgttgtcga aatcgacggc aagaatattt
    21481 atgcgcaaa
//
LOCUS       DAJTPG010000065        21248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTPG010000065 DAJTPG010000000
VERSION     DAJTPG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            13..786
                     /locus_tag="OO230_002547"
                     /pseudo
     CDS             13..786
                     /locus_tag="OO230_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(846..2144)
                     /gene="eno"
                     /locus_tag="OO230_002548"
     CDS             complement(846..2144)
                     /gene="eno"
                     /locus_tag="OO230_002548"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5692258.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2232..3869)
                     /gene="pyrG"
                     /locus_tag="OO230_002549"
     CDS             complement(2232..3869)
                     /gene="pyrG"
                     /locus_tag="OO230_002549"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5692259.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4097..4888)
                     /gene="mazG"
                     /locus_tag="OO230_002550"
     CDS             complement(4097..4888)
                     /gene="mazG"
                     /locus_tag="OO230_002550"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5692260.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4984..7218)
                     /locus_tag="OO230_002551"
     CDS             complement(4984..7218)
                     /locus_tag="OO230_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5692261.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7266..8567)
                     /gene="rlmD"
                     /locus_tag="OO230_002552"
     CDS             complement(7266..8567)
                     /gene="rlmD"
                     /locus_tag="OO230_002552"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5692262.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8624..11380
                     /gene="barA"
                     /locus_tag="OO230_002553"
     CDS             8624..11380
                     /gene="barA"
                     /locus_tag="OO230_002553"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5692263.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11612..12952)
                     /gene="gudD"
                     /locus_tag="OO230_002554"
     CDS             complement(11612..12952)
                     /gene="gudD"
                     /locus_tag="OO230_002554"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5692264.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12973..14313)
                     /locus_tag="OO230_002555"
                     /pseudo
     CDS             complement(12973..14313)
                     /locus_tag="OO230_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
     gene            complement(14315..15667)
                     /gene="gudP"
                     /locus_tag="OO230_002556"
     CDS             complement(14315..15667)
                     /gene="gudP"
                     /locus_tag="OO230_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5692265.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16101..16550)
                     /locus_tag="OO230_002557"
     CDS             complement(16101..16550)
                     /locus_tag="OO230_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5692266.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16568..17350)
                     /gene="truC"
                     /locus_tag="OO230_002558"
     CDS             complement(16568..17350)
                     /gene="truC"
                     /locus_tag="OO230_002558"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5692267.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17350..17679)
                     /locus_tag="OO230_002559"
     CDS             complement(17350..17679)
                     /locus_tag="OO230_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5692268.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18301..18846)
                     /gene="syd"
                     /locus_tag="OO230_002560"
     CDS             complement(18301..18846)
                     /gene="syd"
                     /locus_tag="OO230_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5692269.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18914..19762
                     /gene="queF"
                     /locus_tag="OO230_002561"
     CDS             18914..19762
                     /gene="queF"
                     /locus_tag="OO230_002561"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5692270.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19874..21238
                     /gene="ppnN"
                     /locus_tag="OO230_002562"
                     /gene_synonym="ygdH"
     CDS             19874..21238
                     /gene="ppnN"
                     /locus_tag="OO230_002562"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5692271.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5669 c         5065 g         5286 t
ORIGIN      
        1 gcccaaccaa ctattgtccc acaattacag caacaagtta cgggtcttac gagtagctta
       61 aactcacaag aaaagaaaga actgacgcac aagttagaat ctattttcaa taatacccaa
      121 gtgcaaattg ctgtattaat tgtttccaca accaaagatg agacaattga acaatatgct
      181 acaagagttt ttgacaattg gcgtttagga gatgccaaac gtaatgatgg gatactgatc
      241 gttgttgcct agtcggatcg cactgtccgc atccaggtag gttatgggct ggaagaaaag
      301 gtaaccgatg ctctggctgg agatatcatc cgtagcaaca tgatacccgc atttaaacaa
      361 caaaaattag ctaagggatt agagttagct ataatcgctt tgaataacca actcacttca
      421 caacaccaat atccggctaa tccttcagaa agtgaatcaa cgtcttccac tgatcattat
      481 tactttgcta ttttttgggt atttgcagtg atgttcttcc ctttctggtt ttttcatcaa
      541 ggcagtaatt tttgtcgcgc atgtaaaagt ggagtctgta tttcagcaat ccatctttta
      601 gatttattcc tgttctccga taaaactttt tccattgctg tattttcctt cttttttact
      661 ttcaccatat ttatggtctt tacctgttta tgtgtacttc agaaaagagc atctggtaga
      721 agttatcatt cagacaacag cggttccgct ggaggctcaa gtggcggcgc atccggccgc
      781 tggtaactcc accataaaaa atgccagccc gaaggctggc attatcaaat cagataaagt
      841 cagacttatg cctggccttt gatctcttta cgaccgttgt acggtgcttt ttcgcccaga
      901 gcttcttcga tacgaatcag ctggttgtat ttagcaacac ggtcagaacg gctcatagaa
      961 ccagttttga tctggcctgc agcagtacca acagccagat cagcgatggt agcatcttca
     1021 gtttcaccag aacggtgaga gataactgca gtgtagccag catctttcgc catcttgatt
     1081 gcagccagag tttcggtcag agaaccgatc tggttgaatt tgatcaggat ggagttagcg
     1141 ataccttttt cgataccttc tttcaggatc ttggtgttgg ttacgaacag gtcgtcacca
     1201 accagctgga ttttgtcgcc cagaactttg gtctggtatg cgaaaccgtc ccagtcagat
     1261 tcgtccagac cgtcttcgat agaaacgatc gggtactgtt tggtcagttc ttccaggaag
     1321 tgagtgaatt cttcagaggt gaacgctttg ttgccttcgc cagccagaac gtatttacca
     1381 tctttgtaga attcagaagc tgcgcagtcc atcgccagag tgatgtcttt gcccagttca
     1441 taaccagcag ctttaacagc ttcagcgata acagccagag cttcagcgtt ggaacccagg
     1501 ttcggcgcat agccaccttc gtcaccaaca gcagtgttca tgcctttcgc tttcagaact
     1561 tttgccaggt gatggaaaac ttcagaaccc atgcggatgg cttctttcac agttttcgcg
     1621 ccaaccggct gaatcatgaa ttcctggata tcaacgttgt tgtcagcgtg ctcaccaccg
     1681 ttgatgatgt tcatcatcgg aaccggcatg gagtatttgc ccggagtgcc gttcagttca
     1741 gcgatgtgct cgtacagcgg cataccttta gcagctgcag cagctttggc gttagccaga
     1801 gatacagcca ggattgcgtt cgcgccgaat ttggatttgt tttcggtgcc gtccaggtcg
     1861 atcatgatct tgtcaatgcc agcctgatct ttagcatctt tgccaatcag cgcctgagcg
     1921 atcgggccgt ttaccgcagc aacagctttg gttacgcctt tacccaggaa acgggatttg
     1981 tcgccatcgc gcagttccag agcttcacgg gaaccagtag aagcacctga cggagcagct
     2041 gccataccga cgaaaccacc ctccagatgt acttcggctt caacagtcgg gttaccacgg
     2101 gagtcgatga tttcacgacc gatgattttt acgattttgg acattaggtt ttcctcaagt
     2161 cactagttaa actgaaactc cagacaaaca acgcgtaccc aaggtacgcg ttgccgttct
     2221 aactttttta cttacttcgc ctgacgtttc tggaactcgc tggcggcttt cacaaagcct
     2281 gcaaacagcg ggtgaccatc acgtggagta gaagtaaact ccggatggaa ctggcaagcc
     2341 acgaaccacg ggtgattcgg aacttcgatg atctcgacca actgatcatc cccggaacgg
     2401 cccgcaacgc gcagacctgc atcttcaatc tgtttcaaca gcatgttgtt gacttcgtaa
     2461 cggtgacgat gacgctcaac aattgtcggc gcattgtata gctggcgaac caggctatcg
     2521 tcaaccaact ggcactgctg tgcgccgaga cgcatggtac cgccgagatc gctcttctcg
     2581 ctacgaactt caacgttgcc gttttcatcg cgccactcgg taatcagcgc cacaaccggg
     2641 tacttacagt ctggcacaaa ttctgtagag ttggcgttct ccatgttggc aacatggcga
     2701 gcgtaatcaa ttaacgccac ctgcataccc aggcaaatgc ccagataagg aatattgttc
     2761 tcacgcgcaa aacgcgcggt cgtaatcatg ccttctacgc cacgatagcc gaaaccgcca
     2821 ggtacgagga ttgcgtccag acctttaagg atttcaacgc cgcgcgtttc aacatcttgt
     2881 gaatcgatca gtttgatgtt gacgctgaca cgattcttca gcccaccgtg tttcagtgct
     2941 tcgatcactg atttataagc atccggcagt tcaatgtact tgccgaccat accgatggtg
     3001 acttcactta ccggattcgc ttcttcgaag ataacctgtt cccattcgga cagatttgct
     3061 tccgggcagt ttaagctgaa tcgtttacaa atataatcgt ccagcccctg agatttcaac
     3121 aggcccggaa ttttatagat ggaatcgacg tctttcagag aaataaccgc tttttccgga
     3181 acattacaga acaatgcaat cttcgcacgt tcgttcgccg gtacagcgcg atctgaacga
     3241 caaatcagga tgtcaggctg gataccgatg gagagcagct cttttacaga gtgctgagtc
     3301 ggtttggttt tgacttcacc agacgctgcc atgtacggca ccagcgtcag gtgcataaac
     3361 agagtgtgct cacggccaat ttcaacagcc atctggcgaa tagcttcgag gaacggcaag
     3421 gattcgatat cacctactgt accgccgatt tctaccagta ctacgtcatg accttcgcca
     3481 ccttccagca cgcgctcttt gattgcgtta gtgatgtgcg gaataacctg cacggttgcg
     3541 ccgaggtagt caccgcggcg ttctttacgc agaacgtcag agtagatacg acccgtggtg
     3601 aagttgttgc ggcggctcat tttggtacga atgaaacgct cgtagtgccc caggtccaag
     3661 tcggtttcag cgccgtcttc agtaacgaac acttccccgt gttggattgg gctcatagta
     3721 cctggatcga cgttgatgta aggatccagt ttcatgatgg tcacattgag gccacgggct
     3781 tcaagaatgg ctgcgaggga ggctgcggca atgcctttac ccagagagga tacgaccccg
     3841 ccggtcacaa aaatatagtt cgttgtcatg ctgaacctga gaagttaggt tgaaaagacg
     3901 atggaataac caagacggga aagcagtata cccgaacatg acctgtgcca caaactttca
     3961 ttatccctcc tcttcgccag cgcactattg aaatcaggag tgagaaaata gcccctttgg
     4021 ggtaaatgtt tttgacgcaa atcaagcgct tgtcatttaa aaaatgacac aaatggcgct
     4081 tgaccgcgta attcccttag agatcaattt cctgccgttt tacctgttgc cagacttctt
     4141 ccattgtttc gaggtcaaca cctgtcattt ccagtccacg cgcggcaaca atacgctcca
     4201 cttcgcgaaa acgacgctcg aatttttcgt tcactttttg caatgcgatt tctgctttcg
     4261 tccctaaatg gcgagccaga ttaaccgtgg caaacagcag gtcccccatt tcctcctcca
     4321 gtttagcctg gtcgacaaca gcctgccgcg cctcgtacat cacctcgtcg atctcttcgt
     4381 agactttatc aaccaccggg ccaagcgtcg tccagtcgaa gccaacgttg gcgcaacgtt
     4441 tctggatttt ttgcgcccgc attaaagccg gtaaactacg aggaatatcg tccagcgccg
     4501 aatgctgcgc tttctgcgcg cgctcttcgg ttttgatttg ctcccaacgg gcaagcactt
     4561 cactactgtt ttcggcagaa ctatcagcaa aaacatgcgg atggcgacgc tctaatttat
     4621 cgctaatagc agcgcaaata tcattaaagt caaagcgccc ttcttcctga gccatttgcg
     4681 cgtaaaacac cacctggaat agcagatcgc ccagttcacc gcgaagatcg tcaaaatctt
     4741 cacgggcgat ggcgtccagc acttcgtagg tttcttcaag ggtgtaaggc gcaatggtgg
     4801 caaatgtctg ctctttatcc cacgggcagc cgttttccgg atcgcgcagg cgctgcataa
     4861 tagtgagcaa acggtcgatt tgattcattg aattgtcctg aaaattgcgg gtctgtcagg
     4921 tgtaatacct tcgagtaaaa gatatcgctg gatacggctg acgccatatc cagctatgta
     4981 agactaactc ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt tgagtttacc
     5041 cagcacgcgc cccagcactt gcaggttgta aatctcaatg gtcatgtcga tggtcgccag
     5101 ttgctgtttg gtgtcgctac ggctggcaac gccaagcacg ttcaccttct cgttggcgag
     5161 aatggtcgtg atatcacgta acaacccact acgatcatta gccaccacgc ggaccaccag
     5221 cgaatatccg gcggagtagc tctcacccca taccgcgtca acaatgcgtt ctggcgcatg
     5281 ggagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac cgcgtccctg
     5341 ggtaatgaag ccgacaatct catctccagg aatcggctgg cagcagcgcg cgatgtggtg
     5401 catcaggtta ccaacacctt caaccaccac acgaccgtta tctttactgc ggttttgcgg
     5461 tgtgtagctt ttttgctgaa gctgtttcag cgcggcggcg tcctgctctt cggcactcgg
     5521 cttattaaat tgcgattgca ggaagttcac catctgattg agacggatat ccccgccacc
     5581 aatcgccgcc agcaactcgt cgacatcatt gaagttgtaa cgcggcagca gatgtttttc
     5641 tgcttctttc aggctgatcc ccagatgttc cagctcgtcg tcaaggattt gccgcccagc
     5701 cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg aacgcccacg
     5761 gctggttgtg acgtaaccga ggtttgggtt taaccagtca cggctggggt tcggctgttt
     5821 ctgggtgata atttcaatct ggtcgcccat ttgcagctgg taggtgaacg gcacaatgcg
     5881 cccgccaatt tttgccccga tgcagcggtg tccgacatca ctgtggatgt ggtaagcgaa
     5941 gtccagcggc gttgatcccg caggcaaatc aacgacatca cctttcggcg taaagacata
     6001 cacccggtcg tcaaacacct gactacgtac ttcgtcgagc atttcgccgg aatcagccat
     6061 ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc cggtcttcat gtcccgaacg
     6121 tgcgccgcca gctgccgcgc cctctttata tttccagtgc gcagcaacac ccaactccgc
     6181 atcttcatgc atctgtttgg tgcggatttg gatctcaacg gtttttccac ccggccccag
     6241 aaccacggta tgaatagact gataaccgtt tggtttcggg ttagcgacgt aatcgtcaaa
     6301 ctcatccggc aggtggcgat agtgagtgtg cactatcccc agtgcggcat aacaatcctg
     6361 taaacgctcg gcgacaatac gtaccgcacg cacatcaaac agctcatcga aggcgaggtt
     6421 ctttttctgc attttgcgcc agatgctgta gatgtgtttc ggacgaccat acacttcagc
     6481 tttaacgcct tcagctttca tctcagcgcg cagatggccg acaaactctt cgatatagtg
     6541 ttcgcggtcg agacggcgtt catgcagcag ttttgcgatg cgtttgtatt cggtcgggtg
     6601 gagataacgg aagcagtaat cttccagctc ccatttcagt tgcccaatcc caagacggtt
     6661 cgccaacggc gcgtagatat tggtgcactc ttttgccgcc agtacacgtt catcttccgg
     6721 cgcatctttt acttcgcgca gatgagcaat acgctccgcc agtttgatga ctacgcagcg
     6781 aaaatcatcg accatcgcca ataacatccg gcgaacgtta tcgacctgtt cggaggaaac
     6841 agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca cgccgtgaat
     6901 aaggttaaca accgacttac cgacgctctc acgcagcaca tcttcgctga ctacgttggc
     6961 atcagccaga gggaacagca gcgccgcccg cagcgtgtca atgtccatac ttaatgtcga
     7021 gaggatctcc accatctcaa caccacgcca caataacaga ctggcatccg gatgcccctg
     7081 cgtctgttgc agacaatacg cccaggtttc ggctaagcac tcacacgact tctggctggt
     7141 aatacccaga cttgcgatcc atttttccgg atcaaattca ccagccttat tgatatgtgc
     7201 acttcttacc gcaaccatcg tcctctcctt tagggaccag acctgccgaa atcggcaaat
     7261 cgtaactatt taacgcgcga gaaaagtacc atcgattcca gatgtcccgt gtgtgggaac
     7321 atatccagca tcgccagtcg cgcaatggta tatcctgctt ttaataacgc ttcgctatcc
     7381 cgagccagcg ttgcagggtt acaggataca taaactatac gaataggttc cagttttata
     7441 atttgctgca taacacctgc ggcacctgct cgcgccgggt ccagcaacac tttatcgaag
     7501 ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa gattttcgtg ataaaacgtc
     7561 acattctgta agccattaag acgcgcattc tgctggcctt tttccaccag cgcaggaaca
     7621 ccttctacac cgaccacact ggcagcttgt gtcgccaatg gcagtgtaaa gttgcccata
     7681 ccgcagaaca gatccagtac gcgatcttca ggttgcacat ccagccattc caacgcacgc
     7741 gctaccattt tttggttcac acccgcattg acctgaataa aatcgcgcgg gctaaaagtt
     7801 aagcgcaacc cgtttgagtc ataccagggc atctcaccag agacggtttc gagtatctca
     7861 ctatcggggg cgagatacag atccaggcct tcagaatgcg aaaagcgttc cagtttttcg
     7921 cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc gctggttgcc
     7981 tgtaccagtt caacatgacc aagatggcgc atagcttgta agctgcccag acatgccctg
     8041 actttgggca gcaatgcttc aagttggggc gctaaaatgg ggcattgttt gacgtcgaca
     8101 atgtcactgg agcccgcttt gcgaaacccc atctgaagtt gctgtgtttt cggtaagtag
     8161 ttcagactta aacgcgcgcg gcggcgatag ccccagggaa catcggcgat cacttcagag
     8221 acatcgtgtt tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg taaatccacg
     8281 ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat gaggacagcg tggcgtttcg
     8341 cgttccgggc tatcgcttaa ccggcgtacg actttagcgc gggcatactg ttttttatct
     8401 tcagtaacag caacttccgc gttttcctgc ggcaataatc cggggataaa taacgttttg
     8461 ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga aacggttatg
     8521 atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg cgagactttc
     8581 tcaatttaac agtgtgacct taattgtccc ataacggaac tccatgacca actacagcct
     8641 gcgcgcacgc atgatgattc tgatcctggc accgaccgtc cttattggtt tattgctgag
     8701 tatctttttc gtcgtgcatc gctataacga cttgcagcgt caactggaag atgccggtgc
     8761 tagcattatt gagccgcttg cagtttctac tgaatatggc atgagcctgc aaaatcgcga
     8821 atctatcggt cagttaataa gcgtactgca tcgtcgccat tccgatattg ttcgcgcgat
     8881 ttcggtttat gatgaaaata accgactctt tgtcacctcc aattttcatc ttgatccctc
     8941 atcaatgcag ctcggcagca acgtgccgtt tcctcgccag ctcactgtca ctcgtgacgg
     9001 cgatattatg atcctccgca cgccgattat ttctgagagt tactcccccg acgaatcgcc
     9061 cagtagcgat gccaaaaata gtcaaaatat gttgggatat attgcgcttg agctggatct
     9121 taaatcggtt cgcttgcagc aatataaaga gatctttatt tcctgcgtga tgatgctgtt
     9181 ttgtatcggt attgcgctta tttttggctg gcgcttaatg cgcgatgtaa ccggtccgat
     9241 tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca gccgggtgga
     9301 aggatttatg ctcggcgagc tggatatgct gaaaaacggt atcaactcga tggcaatgtc
     9361 gctggctgct tatcacgaag agatgcagca caatatcgac caggcgacgt ccgatctgcg
     9421 tgaaacgctg gagcagatgg aaattcagaa cgttgagttg gatctggcga aaaagcgcgc
     9481 ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat atgtcacacg agctgcgtac
     9541 accactgaat ggtgttattg gctttacccg cctgacgctg aaaacagaat taacaccaac
     9601 gcagcgcgat cacctgaata cgattgaacg ttcggcaaat aatttgctgg caattattaa
     9661 tgatgttctc gacttctcga aactggaagc aggtaagctg attctggaaa gtattccatt
     9721 cccactacgc agcacgctgg atgaagtcgt tactctgctg gcacattctt ctcacgataa
     9781 agggttggaa ctgacgctca atattaaaag cgacgtgcct gataacgtga tcggcgaccc
     9841 actgcgatta cagcaaatca tcactaacct ggtggggaat gcaattaaat tcaccgagaa
     9901 tggcaacatt gatattctgg tagaaaaacg tgcgctgagt aataccaaag tgcagattga
     9961 agtgcagatt cgggataccg gcattggtat tcctgaacgc gatcaatcgc gcttattcca
    10021 ggccttccga caggctgatg ccagtatttc ccgccgtcat ggtggcaccg ggctggggct
    10081 ggtgattaca caaaaactgg ttaatgaaat gggcggcgat atttcgttcc atagccagcc
    10141 gaatcgcggt tcaactttct ggttccacat taatctcgat ctgaacccga acattattat
    10201 cgaagggcca tccatccagt gcctcgcagg taagcgcctg gcctatgtcg aaccaaactc
    10261 cgcagcagcg caatgcacgc tggatatttt aagtgaaacg ccgctggaag tggtttatag
    10321 cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg atgttgttag gcatcgcggt
    10381 gaccttccgc gagccgctaa caatgcaaca tgagcgatta gcgaaagcgg tatcgatgac
    10441 cgatttcctg atgctggcac ttccttgcca tgcacaagtt aatgctgaaa aactcaagca
    10501 agatggtatc ggcgcgtgtc tgctgaaacc attaacacct acgcgcctgt tgcctgccct
    10561 gacggaattt tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg aaagtaagct
    10621 ggcaatgaca gtcatggcgg ttgatgacaa ccccgctaac ctgaaactta tcggcgcatt
    10681 gctggaagat atggtgcaac atgtggaact ttgcgatagc gggcatcagg cagttgaacg
    10741 ggcgaaacag atgccgttcg atttgatctt aatggatatt caaatgccgg acatggatgg
    10801 cattcgggcc tgcgaactca tccaccaact cccgcatcag cgacaaacgc cggttatcgc
    10861 ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg cttggcgcag ggatgagcga
    10921 ttatctggcg aaaccgattg aagaagagcg attgcataat ttgttgttgc gctacaagcc
    10981 tggcagcggt atttcgtctc gcgtcgtgac gcccgaagtc aacgaaattg tggtgaaccc
    11041 gaatgcgacc ctcgactggc aactggcact acgccaggca gcaggaaaaa ccgatttagc
    11101 gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca aagttgagga
    11161 acagctggct ggagaaaacc cggaaggcct ggttgatttg attcataaac tgcatggcag
    11221 ttgcggctat agcggcgtac cacgtatgaa gaatctctgc cagctgatcg aacaacagct
    11281 acgtagcggt actaaagaag aagatttgga gccggagctg ctggaactgt tggacgagat
    11341 ggataatgtc gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt gtcgagtagc
    11401 aatcggcttt ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt ttatgccgga
    11461 tgcggcgtta acgccttatc cggcctacaa aaccaattaa attcaatgaa ttaaaaaatg
    11521 atgtagacct gatacatagc acaacaggct attttgcgtt tagcatcagt ctcaaaccgg
    11581 ctccagatag agccggtttt ggttttctgt cttaacgcac catgcacggg cgcttgttat
    11641 cgaacgtcca gccaggaatc agatactgca ttcccatcgc atcgtcacgc gcgccaagcc
    11701 cgtgtttctg atacagctca tgggctttca tcacttgatc catatcgatt tctacaccca
    11761 gccccggttt ttctggcacc tgtaccagcc cgcctttgat ctcaaacggt tctttggtca
    11821 ggcgctgatt accttcctgc cagatccagt gcgtatcaat agcagtaatt ttacccggtg
    11881 cagcggcggc aacatgggta aacatcgcca gggaaatatc gaagtggttg tttgagtgtg
    11941 aaccccaggt caggccaaat tcatggcaca tttgcgccac acgtaccgaa ccttgcatag
    12001 tccagaaatg cggatccgcc agcgggatat caacggattg taacgagagc gtatggccca
    12061 tctgccgcca gtcggtggcg atcatattgg ttgcagtcgg caggccagtc gcgcgacgga
    12121 actctgccat cacttcacgc ccggagaaac cttgctccgc accacacgga tcttctgcat
    12181 aagccagcga acctttcagg tatttaccaa ttttaattgc ttcgttcagc gaccaggcac
    12241 cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc acaatagact
    12301 cggcttcttc ttccccggcc agtacaccgc ctttcagttt gaaatcgttg aagccatatt
    12361 tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct tcttcatgac
    12421 gcaggcgata ccagtcgcat gagtcatccg gctggctttg atacggcaga ggtgtggctt
    12481 tgcgattacc gacgaagaac agataaccga gcatttcgac ttcgctacgc tgttgaccat
    12541 cgcccagcag cgatgccacg tttaccccca gatgctgccc cagcagatcc agcattgccg
    12601 cttctatccc ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc aaaccgcgcc
    12661 cactagcatc acgatcggca aaagtattac gcaccagcgt cagaacgttt ttgtattcac
    12721 ccagcgtttt acctaccacc agcggaatcg catcttccag cgttttacgg attttctcgc
    12781 cgccgggaat ttcccctacg ccagtgtgac cggaattatc tttgataatc acaatattac
    12841 gcgtgaagaa cggtgcgtgt gcaccactca gattcatcag catactgtca tgacccgcca
    12901 ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc atatttatgt
    12961 cctttttcag aatcaatgac ggccgaaaac gggacgttta cggtcaaagg tccagccggg
    13021 gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg caaggcagac gtttataggc
    13081 ctcatgtgcc ttttgtacct gttcccagtc cagttccacg cccagaccgg gcgcatcagg
    13141 aacggcaatt tttccgtttt taatctccag cggatttttg gtcaggcgac aatcgccctc
    13201 ctgccaaatc cagtgggtat cgatagcggt aggattaccc ggtgccgccg cgcccacatg
    13261 ggtaaatatc gccagagaga tatcgaaatg gttgttggaa tgtcagcccc aggtcagccc
    13321 ccagtcgtcg caaagctgcg ccacgcggac tgcaccggaa agcgtccaga agtgcggatc
    13381 ggcaagtgga atatctaccg cattgagcat caccgcatga cccatttcgc gccagttggt
    13441 ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgt cgaaactccg ccatcacttc
    13501 acgtccggag aagccctgtt ctgcgccgca tggatcttcg gcataggtaa gaacatcatt
    13561 cagccctttg cataaagaaa tggcttcatc aagcagccat gcaccgttgg gatcaacggt
    13621 aatccgcgca tccgggaagc ttttcttcaa tgcacgaaca gtgtcgattt cttgctcgcc
    13681 aggtaacacc ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct gtgaggcttc
    13741 cgccagacgc acaacggctt cgctgttcat cgctttctga tggcgcaact gataccactc
    13801 atggttgccc ggcgtatttt ccagataagg aagatcggtt ttggtccgat cacctatata
    13861 aaacagataa ccgaggacgg taatagtctc gcgttgcttg cctggcccca acagttcgca
    13921 gaccggaaca ttcagcgcct tacctagcag gtcaagcaag gcggcttcca gcgccgccac
    13981 ggcattaacg cgcaattcaa aagtccaggc acctttgcca aaggtatcaa aatcggctgc
    14041 ctgattacct ttatgtacct gctgaaccac tttattcagg cgcgcaactt cctggcccag
    14101 aaccatcgga atagcatcga ccagtgtttg ataaatcacc tctccgcccg gcgcttcacc
    14161 aatgccggta tgcccggcgt tatcggtgag taccacgata ttgcgggtga agtatgcgtt
    14221 atgcgcacca ccaatattga gcaacatgct gtcatgtccg gcgaccggaa tgactttcat
    14281 atcagtaata acaggactgg attgtgtcgc catcttattg ccccgcgaca ggtttcagct
    14341 cgatacgctt gatatcgccc accagcacca ggtagctcag taccgcgatt agggcatgaa
    14401 caccaacata aatcagcgcc ccattaaacg agccagtcgt gccaacgata taaccaattg
    14461 cgattggcgt gacgatgcca gaaatgttac cgaacatgtt gaacaggcca ccggaaagac
    14521 cgctgatctc ttttggcgcg gtatctgcca ttactgccca acccagcgcc ccgatgccct
    14581 taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg acgtagttgc
    14641 agaacaccat caccatcgac agcaacatgc ccattacgat cggtgtttta cgcgcaatgt
    14701 tcagcgatcc cgtgcggcgc atcagccagt cggaaataat tccacccagc acaccgccga
    14761 taaaaccgca aaccgccgga acggaagcca caaagcccgc tttcagaatc gacatcccac
    14821 gcgcctgcac cagataaacc ggaaaccagg taatgaaaaa gtaagtcagg gcgttgatac
    14881 agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagctgt ttgatctgcc
    14941 cccacttcac gctgaacggc actttaactt tggtgctttg ctgatccata ttgatcagcg
    15001 cgccacccgc ggcgatatac tccagctctt tctgatttac ccccggatgt tgatttggct
    15061 cgtggatgac tttcaaccag ataaagctga tgacaatgcc cagaccgccc ataaagaaga
    15121 agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg aagatcaccg
    15181 ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc tgcaccggaa
    15241 accaggccgc aacaatgcga ctgttgccgg ggaaagatgg cgcttcagca agcccgacca
    15301 ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc actaaagata tcgacgaagc
    15361 cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg cgttttgaac
    15421 caaaacgatc cagcaaccaa ccaccaggga tctgcccgat aacataagcc catgagaagg
    15481 cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc atttccgaac
    15541 cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata aacaacatca
    15601 ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc tgacttaaag
    15661 aactcattgt tgcactcctg aaaattcgcg ttagccacgc tcactctgga ctgcgacatc
    15721 gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt tttataggtc gttcgccgaa
    15781 tacggcgcgt gtttatatct ggcaatagca gtataaaaag cgcgccatag cggctcaccg
    15841 tgcaacaaca caacattaat gcgttcaatg aggcccgatt ttggcattag ccctggacgg
    15901 tggaatccac ttcacggaaa tgaaaacaag aacaagaaag gaagggtaaa aacgaagaaa
    15961 taaagaagag tatgaaatgg atcgcttgac tctaggcaaa cgccagtaaa aatccgcgct
    16021 atgaagcagt ttttactggc atttgcctga aaagattcga ttcagcaccg ctaaaacgac
    16081 atttaccgct cgctgacata tcaggacaac agcgtgcccc actgttcgac ccacggattt
    16141 gattccgttt ccggttccgg gttctcactg gcgtcaatca acagcatttc gccaacccgc
    16201 tgagcgctct gttcctgcaa caaggcatcg aactgtttgc cgccattgca aaaatttaca
    16261 taactgctgt cgccgagcgc aatcacgcca taacgcagat tcggctggaa gcccagacta
    16321 tctttgattc cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg
    16381 gacgtaacca ccagaacata cttatcctga tagggcagcc agtcgcttaa ttcaggatct
    16441 tcaaataccg ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc ttccgccact
    16501 aacagcgaat ttccgtacat ggtgccgaca aaaataccaa tttccgccat accgttttct
    16561 ccctggatta ggaacttatc tctccatcct gacccgatgg cgcactgaac tcaacccttt
    16621 cattttcagg aagcagaccg cgccagccaa attgtgataa cgcctgcatc caggtgtcgt
    16681 ccaggcccgc gtgaatagtc agcggctcgc cagtaaaagg atgcgtcaat gacaactgac
    16741 tggcatgtag cattaaccgc tggaggccaa aatgctcagc accgctgcga ttctggcgta
    16801 aatcgccatg tttgctatcg ccaataatcg gatgacgcaa atgggcaaga tgtcgtcgaa
    16861 gctgatgttt gcgtccggtt ttcggctcca gttccaccag gccgtagcgc gtggtcgggt
    16921 aacgtccggt cgctaccggc atttctacgg tcgccagacc gcgataatgc gtcactgctg
    16981 gctgcgggcc tttatcttcg cgggcaaatt tatcagcgat tttgtccagt tcttccacca
    17041 gtggataatc caacaccgct tcttccatca accagccgcg cacaatcgca tggtaacgtt
    17101 tctggatttg gtgctgttca aactgttgtg ccagcagccg tccggcctcg ctggataatc
    17161 ccatcaacaa cacaccagaa gtgggtcggt ccagacgatg agcagtaaaa acatgctgtc
    17221 ctatctggtc acgcacggtt tgcatgacca ctactttctc gtcgcgatcc agccagctac
    17281 ggtgaaccag ccagccggag ggtttattta ccgcaaccag ccattcatcc tgatagagta
    17341 tttccagcat tagctcgcat catccgcaaa aagagcatcc agtttttcca actcagccag
    17401 aataagcgcg cgttgcggat ggtccgtcgc cagcgccatt tcataatagg gtgcaacagc
    17461 aaaagcgccc ggtaacggct ggttgttatt taacaaatcg tgcattcgcg ggatcagccc
    17521 ccactgcaac cactccagtg gttccatggt gtccataaag aacggttggg tactattaaa
    17581 ttgatgcggc tggggttcat cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag
    17641 cgcctgcaac tggagacgaa cgcggtcatg agtggtcata aagcaacctc aataagaaaa
    17701 actgccgcga aggatagcag gaataaaaaa agggagcact gtattcacag tgctcccggt
    17761 tcgtttcgca gcattccagc tacttttgtt gctccctgct catccttgac aacttttcct
    17821 ctggccttgc ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac caccccgatg
    17881 tgatacttca tcctgaagtg tccctggcct tcctgaccca ccgaatcatc ctgaccggtt
    17941 ctcattctcc atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc
    18001 cagaagcctt tccctgaaac accatcctgg tgtgtcctgc agaagtgtca tcatcctgat
    18061 gttcacttcg ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt cgcgaagtct
    18121 tacaaggcgc ttacagacaa tgccttaagg aaaatttcat atgaaagtta agaattaaca
    18181 atcaacattt tgattttaaa tgaaaataac gattcttatc tttttaagta ttcgcttatt
    18241 tcctggcgat ctcctacaga gtctgtaaga gatctctcac aaccagatgg ggtatttgga
    18301 ttacagaaga ggcttaagtt gattaaggaa ttccgcaaga tttgaggcta aattcgtccg
    18361 tttacgtgtt cccagcgtct ctttgcacac ttcaccggag agattacaca ccgagatgac
    18421 ctctaactct tcttccagcg tcgcgataaa tagcgtgggc ggcagtttta gccttttctg
    18481 agtcaccaga tgaccaatca gattttcctg cacccgacgg aagtcgtctt cactccaggt
    18541 ttgcagcaat gtcagtttga tatcgccaaa ctgcgcgtgc atatccccgg caaactgagt
    18601 cgtataaaag gtatgaattg tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt
    18661 tacattttgt tccccggtaa acggctgggg ttgccagtat acggcatctt cggtagttga
    18721 aataatgcac ggtgaaggaa caccgtacaa ttcctcactt aacggccagc ttttatgctc
    18781 ttcatgccac gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc
    18841 gtccactaat ttctctcttc acttaaacca gatacacttg tcgtttagtt tatctggttt
    18901 atgacggtga aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc tgactcttgg
    18961 aaaatcaacc gattaccggg atacctatga cgccagccta ctgcaaggcg ttccacgcag
    19021 cctgaatcgc gacccgctgg gtctgaaagc ggataacctg ccttttcacg gtacggatat
    19081 ctggacgctg tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg tcgctgtcgg
    19141 tcatgttgaa cttgattaca ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta
    19201 tctcaacagt tttaaccaga cgcgttttaa taactgggat gaggtgcgcc agacgctgga
    19261 gcgcgactta agcacttgcg ctcagggtaa gattagcgtg gcgttatatc gtcttgatga
    19321 actggaaggc cagccgatag gtcatttcaa tggcacttgc attgatgacc aggatatcac
    19381 tatcgataac tatgaattca ctactgacta tctggagaat gccaccagcg gtgaaaaagt
    19441 agtggaagag acgctcgtca gccacctgct gaaatcaaac tgcctgatca cccatcaacc
    19501 agattggggt tcgatccaaa ttcagtatcg tggacgccaa attgacagag aaaaactgct
    19561 gcgttacctg gtctcattcc gtcatcacaa cgagttccac gaacagtgcg tggaacgcat
    19621 ctttaatgac ctgttacgct tctgccagcc agagaaattg agcgtttacg cacgttatac
    19681 ccgtcgtggc ggtctggaca ttaacccgtg gcgcagtaat agcgattttg tcccatcgac
    19741 cacaagactg gttcggcaat aaattttttc tcaattttgc gtgctggatt cacgcagaag
    19801 gttgtgaaag gtcatcaggc agggctattg taatcaaagg gaatgacgat attcgtccca
    19861 taaggagttt ttcttgatta cacatattag cccgcttggc tccatggata tgttgtcgca
    19921 gctggaagtg gatatgctta aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa
    19981 ctgttcactt gccgtactga actccggtag tttgaccgat aacagcaaag aattgctgtc
    20041 tcgttttgaa aatttcgata ttaacgtcct gcgccgtgaa cgcggcgtaa agctggaact
    20101 gattaatccc ccggaagagg cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa
    20161 cttgttcgcg gttctgcgag acattctctt cgtttacggg caaatccata ataccgttcg
    20221 ttttcccaac ctgaatctcg acaactccgt ccacatcact aacctggtct tttccatctt
    20281 gcgtaacgct cgcgcgctgc atgtgggtga agcgccaaat atggtggtct gctggggcgg
    20341 tcactcaatt aacgaaaacg agtatttgta tgcccgtcgc gtcgggaacc agctgggcct
    20401 gcgtgagctg aatatctgca ccggctgtgg tccaggagcg atggaagcgc cgatgaaagg
    20461 tgctgcggtc ggacacgcgc agcagcgtta caaagacagt cgttttattg gtatgacaga
    20521 gccgtcgatt atcgccgctg aaccgcctaa cccactggtc aacgaattga tcatcatgcc
    20581 ggatatcgaa aaacgtctgg aagcgtttgt ccgtatcgct cacggcatca ttatcttccc
    20641 tggcggtgtg ggtacggcag aagagttgct gtatttgctg ggaattttaa tgaacccggc
    20701 caacaaagat caggttttac cattgatcct caccggcccg aaagagagcg ccgactactt
    20761 ccgcgtactg gacgagtttg tcgtacatac gctgggcgaa aacgcgcgcc gccattaccg
    20821 catcatcatt gatgacgccg ctgaagtcgc tcgtcagatg aaaaaatcga tgccgctggt
    20881 gaaagaaaat cgccgtgata caggcgatgc ctacagcttt aactggtcaa tgcgcattgc
    20941 gccagatttg caaatgccgt ttgagccgtc tcacgagaat atggctaatc tgaagcttta
    21001 cccggatcaa cctgttgaag tgctggctgc cgacctgcgc cgtgcgttct ccggtattgt
    21061 ggcgggtaac gtaaaagaag tcggtattcg cgccattgaa gagtttggtc cttacaaaat
    21121 caacggcgat aaagagatta tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca
    21181 gcatcgtatg aagttgccag gctcagccta catcccttgc tacgaaatct gcacgtaatc
    21241 tcccctcc
//
LOCUS       DAJTPG010000066        21176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTPG010000066 DAJTPG010000000
VERSION     DAJTPG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..345)
                     /locus_tag="OO230_002563"
     CDS             complement(<1..345)
                     /locus_tag="OO230_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HCR5692272.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMN"
     gene            complement(400..1251)
                     /gene="manZ"
                     /locus_tag="OO230_002564"
     CDS             complement(400..1251)
                     /gene="manZ"
                     /locus_tag="OO230_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5692273.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1264..2064)
                     /gene="manY"
                     /locus_tag="OO230_002565"
     CDS             complement(1264..2064)
                     /gene="manY"
                     /locus_tag="OO230_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5692274.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2127..3098)
                     /gene="manX"
                     /locus_tag="OO230_002566"
     CDS             complement(2127..3098)
                     /gene="manX"
                     /locus_tag="OO230_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5692275.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3561..5111
                     /gene="yoaE"
                     /locus_tag="OO230_002567"
     CDS             3561..5111
                     /gene="yoaE"
                     /locus_tag="OO230_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5692276.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5115..6713)
                     /locus_tag="OO230_002568"
     CDS             complement(5115..6713)
                     /locus_tag="OO230_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5692277.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6844..8208)
                     /gene="sdaA"
                     /locus_tag="OO230_002569"
     CDS             complement(6844..8208)
                     /gene="sdaA"
                     /locus_tag="OO230_002569"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5692278.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8392..8970)
                     /locus_tag="OO230_002570"
     CDS             complement(8392..8970)
                     /locus_tag="OO230_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5692279.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(8974..10335)
                     /gene="pabB"
                     /locus_tag="OO230_002571"
     CDS             complement(8974..10335)
                     /gene="pabB"
                     /locus_tag="OO230_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5692280.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10409..10588
                     /locus_tag="OO230_002572"
     CDS             10409..10588
                     /locus_tag="OO230_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5692281.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10708..11067)
                     /locus_tag="OO230_002573"
     CDS             complement(10708..11067)
                     /locus_tag="OO230_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5692282.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11429..11773)
                     /locus_tag="OO230_002574"
     CDS             complement(11429..11773)
                     /locus_tag="OO230_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5692283.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11905..13815
                     /locus_tag="OO230_002575"
     CDS             11905..13815
                     /locus_tag="OO230_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5692284.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13873..14568
                     /gene="tsaB"
                     /locus_tag="OO230_002576"
     CDS             13873..14568
                     /gene="tsaB"
                     /locus_tag="OO230_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5692285.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14608..15189
                     /gene="yeaY"
                     /locus_tag="OO230_002577"
     CDS             14608..15189
                     /gene="yeaY"
                     /locus_tag="OO230_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5692286.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15395..17080
                     /gene="fadD"
                     /locus_tag="OO230_002578"
     CDS             15395..17080
                     /gene="fadD"
                     /locus_tag="OO230_002578"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5692287.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17162..18277
                     /gene="rnd"
                     /locus_tag="OO230_002579"
     CDS             17162..18277
                     /gene="rnd"
                     /locus_tag="OO230_002579"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5692288.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18331..19296)
                     /gene="yeaX"
                     /locus_tag="OO230_002580"
     CDS             complement(18331..19296)
                     /gene="yeaX"
                     /locus_tag="OO230_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5692289.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19352..19969)
                     /gene="yeaW"
                     /locus_tag="OO230_002581"
                     /pseudo
     CDS             complement(19352..19969)
                     /gene="yeaW"
                     /locus_tag="OO230_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20311..20877)
                     /locus_tag="OO230_002582"
     CDS             complement(20311..20877)
                     /locus_tag="OO230_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5692290.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21089..>21176
                     /locus_tag="OO230_002583"
     CDS             21089..>21176
                     /locus_tag="OO230_002583"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5692291.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5235 a         5247 c         5449 g         5245 t
ORIGIN      
        1 gttcatcgct ttgattcggc tatattcaat ccagacattg gcgaagaaaa aacctttttg
       61 tttaaagatg atcttcggaa tgcggatcca gaatatataa aaacccatca gagccagtgc
      121 acttaataac catgttgtta ttagcgcgcc atgattcgtg acgttgttat agataagaat
      181 gacaatcaat ccgacgaaga taacgctatc gatgcgacca cgccggagca aaggaattgc
      241 cagcagggtg gggccgttac ggcggggcat gatgaactga tcgtagatcg cgaaggccag
      301 gagtgcggcg atgaaaagaa tcagtaccag gtccgtgatt gtcattaatc ctccagataa
      361 aaaaacgggg ccaaaaggcc ccggtagtgt acgacagtct tacagtccca gcaggccgca
      421 agcgtaacca gcgataccga tgacgaagaa gccaacgatg atccacagcg ggttaacttt
      481 tttgcgcagc agccacatac aagcaaatgt cagcagcagt ggtacaaggc ctggcattaa
      541 ctggtcaaga atagtttgca cggtggtgac acgggtctgt ccatcctgcc cggtgatggt
      601 cgaaaccacc agcgggatgt tgacatgtgt ccacttgtta accaatgccc ccatgacaaa
      661 caggccgagg atagacgccc cttccgtcag tttttgcagg aagccaccac ccatatcttt
      721 aacgatatcg atacctttgg agtaaccata cgctacgccg tagtaacggg ttgccagacg
      781 caccaggtta aacaggatga agaacagcag cggacctaac aggctgccgc tcatcgcgat
      841 accggcaccc agtgctgcaa ataccggacg tacggttccc cagaagatcg ggtcgcctac
      901 accagccagc ggccccatca aaccgacttt gataccgttg atagcaccgt catcgatctc
      961 tgcgccatta gcacgctgtt cttccagcgc cagggttacg ccgagaatcg gtgcagccac
     1021 gaacggctgg gtgttaaaga actccaggtg acggcgaata gcttgtttac gagcttcgtt
     1081 gttctcaggg tagaggcgac gaattgccgg taccatagag aagcagaaac ccagtgcctg
     1141 catacgttcg aagttccatg aaccctggaa gaggttagaa cgcaggaata cgccacgaat
     1201 atcactttga gtgagttttt tctcggtggt agtttgagtt gtatcaacca tttcgctcac
     1261 ctgttagtcc agttcgttat cgagatcgtt gttaccagct gcctgagcag gcgcaccggc
     1321 tacgcggttg tatttcgggc taagttggat gtagagcact gccataacag taccaatcac
     1381 acccagagca accaggttaa agttggtgaa tgctgcggtt acgaagccga ggtagaagaa
     1441 cggcatcagg tagccagcac gcatcatgtt gataaccatc gcataaccaa ccaccacgat
     1501 catgcccccg gcgatattca gaccattggt caccacttcc ggaatcgcat tcagcatgtt
     1561 ctgtacttcg ctggtaccaa cagacagcgc aacgatgacg gccggaatag ccacacgcat
     1621 tgcttgcagg aacagagaag aaacgtggat ccaggaaatc gctgtcaggt tgccgttatc
     1681 agcagcctta tccgcagcgt gctggaaagc aacggtaata gtacgaacga tgatggtcag
     1741 tacctggccc gcagcggcca gagggattgc cagtgcgata cctgcaccaa tgctctgatg
     1801 acctgcgata accagaatgg tagaaatgat agaagccaga gcggcgtcag gcgcaactgc
     1861 agcaccgatg ttcatccagc ccagcgcgat catttccagc gtaccaccga taataatacc
     1921 ggttttcata tccccaagaa cgatacccac cagggtacac gcgattagcg gacggtgaaa
     1981 ctgaaattca tcgaggattg atcccatacc tgcgatacag gctacgataa ataccagcac
     2041 aatttgaaga gtggtaatct ccattgtact tctcctgttt acgtaagact taagtgtgaa
     2101 aactgaatga taatcaacac aatacgttac ttatcgattt tgctgatcag atccatcatt
     2161 ttcagtttcg gatcggtgga aaccttacgg acttccagct caataccgcg tgcattcagt
     2221 tttttgaacg cctcgatatc tttttcatca accgaaaccg cgttattcac ctgggtttta
     2281 ccctgacgga atgccatacc accgacgtta acagaggtga ttttcacgcc gccttcaacg
     2341 agacgctcta catctgttgg gttggtaaat aacagcatta cgcgttcgcc agcatatttc
     2401 gggttgttgt agacgcgaat cattttggca acatcaacta cgtgtgctgt tacgcccgga
     2461 ggtgcaacct gggtgagcag tgtcttacga acggtatccg cagccacttc atcactaaca
     2521 acaataatac gggagacatt ggtttctttg gtccagcggg tggcgacctg accgtgaatc
     2581 agacggtcgt cgatacgcgc aaggccaata accatgtagt cgtttggccc cattggtttt
     2641 gccggagttg gagccgcttt tggtgctgcg gcagcgggtg ctggcgcggc tttttcaacc
     2701 ggtttggctt tcagtgcttt cacgccttca cggcctgttt ctactgccag cgcaaccagt
     2761 tcatcaaagc tcgggtcatc atcacgggcc attaacgttt ccacgagcat tggaatgtta
     2821 acgcctgcaa tgacttcata atgctctttg tcgacgacaa tgcggctggc agcattgaac
     2881 gggctgcctc cccatgtatc aacgagaaac agcacgcctt tagtggtgtc gagttttgcc
     2941 aactgagcgt tgtatttttc aatcagcgtt tcggcatttt cacctggaac gaaatcgatc
     3001 cagccgacgt tttcctgctc gcctaacagc atttctgccg ttttaagcaa ctgctctgca
     3061 gcccaaccat gtgtgcctat aacaatagca atggtcactt gctacctcct ttattatcgt
     3121 taacacctca acgtgccaga tgtatttttg aatcgtcgtc cacaatcgaa tcgattcaga
     3181 taagggctac aaccaaaaac ccctgcgttt cgcgatttat tttagatatc gaaaaaatta
     3241 ttttatgtga tgaagatccg taatttaact ttcgattatc agaaatttcg aaaggtaaaa
     3301 tatccttgtc acattcgttt gcaaaggaag gtaaatcttt gccaaatcag aggcgtctct
     3361 gatatgttta actcccgttt aacaaccatg gagtataggg cagtagcccg cagtatggat
     3421 cgtcaccgac gtcatttcag catcaggcct tttaacgcct gcctttctgg cactctatgc
     3481 cgcacctttc gtttgcattt tgtcgttacg cctgcattat ttctggcgtc gaatagctat
     3541 tccttaagca ggagcttgtc atggaattct taatggaccc ctcaatttgg gcggggctac
     3601 tcacgcttgt tgttctcgaa attgtgctgg gtatcgataa cctggtcttc atcgccattc
     3661 ttgctgacaa actgccgcca aaacaacgcg ataaagcgcg tttgctgggg ttatcactgg
     3721 cgctgattat gcgtctgggg ctgctgtcgc tgatttcatg gatggtcacg ctgaccaaac
     3781 cgctatttac cgtcatggat ttctccttct ccggacgcga tctgattatg ttgttcgggg
     3841 ggatattctt gctgttcaaa gcaacaaccg aactgcatga acggctggaa aaccgcgatc
     3901 atgattccgg ccacggtaaa ggctacgcca gtttctgggt ggtcgtcaca cagattgtca
     3961 tccttgacgc cgtcttctcg ttggatgcgg taattactgc agtagggatg gttaaccatc
     4021 tgccggtgat gatggcggcg gtagtgattg cgatggcggt tatgttgctg gcatcgaaac
     4081 cgctgacgcg attcgttaac cagcacccca cggtggtggt gctctgtctg agcttcctgt
     4141 taatgattgg tctgagtctg gtggcagaag gtttcggttt ccacattccg aaaggttacc
     4201 tgtatgccgc gattggcttc tcgatcatta tcgaagtgtt taaccagatt gcgcgtcgca
     4261 actttattcg tcaccagtcg actttgccgc tgcgagcgcg tactgccgat gccatcctgc
     4321 gtttgatggg cgggaaacgt caggccaatg ttcagcacga tgccgataac ccgatgccga
     4381 tcccggaagg tgcatttgcc gaagaagaac ggtacatgat taacggcgta ctgacgctgg
     4441 cgtcgcgttc tctgcgcggg atcatgacgc cgcgcggtga aataagctgg gttgacgcta
     4501 atctcggggt cgatgaaatc cgcgagcaac tgctctcttc accgcacagt ctgttcccgg
     4561 tatgtcgcgg tgaactggat gaaatcatcg gtatcgtacg tgctaaagaa ctgctggtgg
     4621 cgctggaaga gggcgttgat gtggcggcga ttgcttcggc gtctccggcg attatcgtcc
     4681 cggaaaccct cgatccgatc aacctgctgg gcgtgctgcg tcgcgctcgc gggagctttg
     4741 ttatcgtgac caacgagttt ggtgtggtac aaggcttggt cacgccgctg gatgtgctgg
     4801 aagccattgc gggtgaattc ccggacgctg acgaaacgcc ggaaatcatt accgatggta
     4861 acggctggct ggtaaaaggc ggtacagatt tgcatgcctt gcagcaggcg cttgatgttg
     4921 agcaccttgc cgatgacgat gatatcgcga cggtcgcggg cctcgtgatc tcggcaaatg
     4981 gtcacattcc ccgtgtgggc gatgtgattg atgtagggcc actgcatatc accatctttg
     5041 aagccaatga ttatcgtgtt gatctggttc gcattgttaa agagcaaccg gcgcacgatg
     5101 aagatgagta agcgttaacg taacgacata atgggcgtga tatgtccatt atgccgggcg
     5161 ggcggcggtt ggctgcccgc cagccatttg ggaaaatcac gtagcggcat cggctgtgcg
     5221 tacaaatacc cttgcaaaat atgcacgcca tgacggcgca aatacttcgc ctgttcttgc
     5281 gtttccacgc cctccgccac cagttcaata ttcagtcttt gccccagcgc gatgatgata
     5341 tcggtcaccg tcgagttaac cgcgtcagaa cctatagctg cggtaaatga cttatcaatt
     5401 ttcagcacgt caggacgtaa tgtttcaagc caggaaaacg agctgttgcc ggtgccgaag
     5461 tcatcaatcg ccagtttgac gtttttacga tgcagctcgc gggcaatccg ataatcaaca
     5521 tccagtaagg catcgcgttc ggtgatttcg aggatcagtt gctgaatcgg gtgagcgcta
     5581 aaccagtact gattgagatc ttttatcagc acaccacggc gaaaatggct gggtgcgacg
     5641 ttaatgccaa catgaaactg actactcatc gggaaaacat ggtgctgacg aatggtttct
     5701 gccatcacat agtgggtcag tggcacaatt aaatgatgtt cttccgcgat aggaataaac
     5761 acatccggtg aaatccagcc ctgacgcgga ttattccagc gcagcaaaat ctctacacca
     5821 atacattgct ggctgcgcgc attaagcaaa ggctgacaga acaattcgaa ctcatgttgc
     5881 gccaggccca gattaatttc gcgggaaaag ctcatccggt aagcggtcgc cagccaggcg
     5941 atatagccca ccagtaaact tagcagcacc gccagcggta attgtgttgg aagatagtgc
     6001 catgccagcg ccgtagcccc cggaccattt acgttaatgg taaaaggaaa gctttgcgaa
     6061 gaaacctgat agttttcatt gtcttcaggt tgcggaaggg aatctaccag cccattacca
     6121 tagagcaaat gccgtttgtc caccgtcagg ctggcgctac tgatttgcgg cagttgtggc
     6181 tcaaggagca ttgccgtcag taagtcgatg ttaatcatct ctatgacccc agcattgcta
     6241 ctgcccgctg caggcgtcca ttgaatcaaa accggactgc ctttaatcag ggcacggtcg
     6301 atcgtcaggc gtaaaagtgg ttgcggtgca ggaagttcag ccagaatgtc cacgacgggc
     6361 acattgcgat aaccaaaaat gctggagcaa taaagtgtgc cgtcttgcac caggccaatg
     6421 gatcgcacag tttggagttt tgccgcctgt ttacgtaatg gcaaatgggc gacagagcag
     6481 ggcagaccaa tcagcggaag caagactttg ctacctgcct gtaggggaag cagtacttta
     6541 tctaattcct ctacagcgtg attggcgaat tgtactaccc gttgttgatt taagttacgc
     6601 tgtgaaataa atcgcacgct cagggtcgaa gcgagcgtaa cgagtgcgca aatcgtacaa
     6661 acaatcattc gattacggcg ataggtttta atgatccgtt gtgctttttg catgcgggcc
     6721 accctgtaag cgtatggcga caaacgcctg acgcaacagt ggaagtgtag tggggaatat
     6781 tacagcagac gagaaagcgg gtataaattc gcccatccgt tgcagatggg cgagtaagaa
     6841 gtattagtca cactggactt tgattgccag accaccgcgt gaggtttcgc ggtatttggc
     6901 gttcatgtcc ttaccggttt cgtacatcgt ttcgatgacc ttatccagcg agacgcgcgg
     6961 tgcactggtg cggcgcagag ccatccgtgc ggcgttaatc gccttcacag aggcaatggc
     7021 attacgctca atgcacggca cctgaacctg ccctgcaacc gggtcgcagg ttaaaccaag
     7081 gttgtgttcc atgccaattt ccgccgccac gcaaacctgt tccgggctac cgcccagcag
     7141 ttctgcaaga cccgcagcag ccattgaaca ggcaacaccc acttcgccct ggcaaccaac
     7201 ttccgcaccg gaaatagagg cgttcatttt atacaatgca ccaatcgcgc ccgctgccat
     7261 aaagtaacgg gtatagatgt ctgggctgac cgattcaata aagtggtcat agtaagccag
     7321 cactgccgga acgataccgc aggcaccgtt ggttggcgca gttaccacac gaccaccggc
     7381 ggcgttttct tcgttaactg ccagcgcaaa catgtttacc cagtcaatga cattcatcgg
     7441 atcgttagac agtttatcgc tggaaactaa catccggcgc agggcagacg cacgacgtgg
     7501 cacgcgcagc gggcctggca gtacaccttc ggtgttcatc ccgcgatcga tacatgcctg
     7561 catggtttgc cagacatgcg cgaaatactc gtcgatctct ttcttgctgt gcagcgccag
     7621 ttcgttctgc atagcgagac cagacagcga atagccggtt tcattacagt acgcgagcag
     7681 ttcggtggca gatttgaacg gatacggcac gctcacttcg ttggcagcat cctgaccaaa
     7741 gtgttcttca tcgacgataa aaccgccgcc gatggaataa taagttttgc tgtagacgac
     7801 ttcatcgccg ttataggcgt ggatttgcat accgttttca tgcagcggca ggttgccgtt
     7861 atgaaaacgc atcccgttgt cgcgcgggaa atccacttca tgccgtccct gtgccagcag
     7921 cagacgttcg cgctctttta cgtcgcgaat aaaaccggga atactgtcga tatccacggt
     7981 ggcaggttcg ttacctgcaa gacccataat aatggcgata tcggtgtggt ggcctttacc
     8041 cgtcagcgac agtgaaccat aaacgtccac ggcaacgcga gtaacgctat ccagtaagcc
     8101 tttttcgacc agatcatcga cgaactgttt acccgccttc ataggcccta cggtatggga
     8161 agatgaggga ccaatcccca ccttaaacat gtcgaataga ctaatcacga taatactcct
     8221 gacaaggtga ctggacttcc agtaacgaac taataacagc gcatagtgta agagggaacg
     8281 cggcgactgg cttaactatt cacatgaatt aaactaatca aaaaccgcga tttaactaat
     8341 attgcgatga ttgtctcaag tagtgttttg tagatgtaaa gataagtata gtcagggggt
     8401 cacaccaatt tgcagcgcca gctcacgaat tatgcctgcg gtcattcccc atacaaaata
     8461 ctgttcgtac caggacagcc atacccgatg tgaatcgcca cggcggtaga tatctaaagg
     8521 gtgataacga cccagatgta atgcctgggc gagcggcatt tcaaacaccg ccgagacttc
     8581 atcttcactg gcgcgatacg gcagatcggg cgggataatg ccgaccactg gggttacttg
     8641 atagccagtt acgctatcga cgggcggcag cacgccgata acttcaacgg cggaaggcgg
     8701 tatagcgacc tcttcttcag cttcgcgcag cgcggcggcg ataactgatg cgtccgtgtc
     8761 atcgactgca cctccaggga atgccacttg tccagcgtgt ttacgcagat gaattgaacg
     8821 ctgagtcagc aacaaccccg gttgcggtcg acggacgatg gggattaaca cggcagcctg
     8881 acgatgattt aggggttccc ggttaatttg cgggcgcaaa agttgaaagc gagataaaaa
     8941 atcatcaagc gtcaggctac ggtattccac gtcttacttc tccagttggc gtaatatctt
     9001 attaacttta tcaaaagttt cctgatattc cgcttcttcc tggctatcgg cgacaattcc
     9061 accgcccgca gagcagtata tttgtccgtt aatggcagtc agcgtgcgga tagtaatgct
     9121 ggtatccatg ttgccgcaaa agctcaaata gccaatgctg ccgcaccagg cattacgtcg
     9181 ctgcggttcc agttcgtcga taatttccat agcccgtact ttcggagccc cggttattga
     9241 gccaccagga aaagctgcgc gcagcagatc gctggcgtgt aactgttctg gtagtcgcgc
     9301 cgttatagtg ctgaccagat gatgcacggc agggaagggt tccaccacga agagctctgg
     9361 tacttttacc gaaccggcta cggcaacacg accgatatca ttacgcatta aatcgacaat
     9421 catcagattt tcggcacgat ctttcgctga gttcgccagt ttttctgctt gtttgctatc
     9481 ttcctgagga tcgggcaggc gtggtagcgt gcctttaatc gggcgagtct ggatttcact
     9541 attatcacaa agaataaacc gctctggcga aaggcttaaa attgcaccct gttcaagacg
     9601 taaaaaagcg ctaaatggcg cgcggttggc ctgattaagc tgaaggaatg cctgccattc
     9661 atcgccagaa taggtcgcat gaaaacgctg ggcgagattc acctgatagc aatcaccgct
     9721 gtgcagatat tcctgtacct ggcgaaattt ttcgccgtac tgctcgtggg tcatattgga
     9781 ttgccagtcg gaagtgagcg tgaaatcttc ctgcggcgag aattgctggc tttccagcca
     9841 ggcccgacgg gcattgacat cattatgact cagcaaagaa actgtatgac gctggtggtc
     9901 gacaatgagc gcccaatcgt agatacccac tgccatatcc ggcagaacga tatcttgttc
     9961 cgcaatttct ggcagtgact caaaacggcg gcccagatcg tagccaaaca accccagtgc
    10021 gccgccctga aatggcaaat cttcgttatg cgttgggcga atgtctgcgc gatccagcac
    10081 ctgctggagc acctgtagcg ggtcatcagt ggtcgttgtg cgtttttcgc tttcactaac
    10141 aacggtttct ttacctaaag tggttaaagt gcaaatcgga tcggcgacca caatatcaaa
    10201 gcggctatac ggatgatcgg catagccgga gtgtaaaagc atcgcccacg gcaggtggct
    10261 taagcgggag aaataaaatt cagcggcgtc ctgacgccag ggtaaagtaa tcacagtggg
    10321 agataacgtc ttcatcagtc ctgactctac tggctatgtg ccgggtagca tgaaataatt
    10381 agcgccaaca atttagcagg agttaacaat gtttgcaggt ttaccttcac tcatccatga
    10441 acagcagcaa aaagctgtcg agcggatcca ggaactgatg gcacaaggga tgagtagcgg
    10501 ccaggcaatc gcgctggtgg cagaagagct gcgtgccaac cactccggcg agctaatcgt
    10561 ggcgcgtttt gaggatgaag acgagtaaca cactaatccg tatcgatttc gttatagaag
    10621 ccgatgatca tttgattgtg agaaaaagga cgctttttat atgtgattaa aagcgtcctg
    10681 acccatttat gctgatataa atttaactca gaccagtgct tttagttctt cctgcatata
    10741 tgttgaaaag tattcaggat ttttaatcac agagcgttca cctgtctcca ttttttttgc
    10801 ccatccaacc attttttccg tgaacccagg gttccagcct tcctggtcag ctcgcgccgt
    10861 aatatcagcg gcactcgcgg gtactcccag ttcttttaaa tatttaaaga ttcccttcgc
    10921 cgtgctttca ttcatcgaac ttggtgttgg cgctgataca tccatggcac caatgaaatc
    10981 cagggcttta tctattacag caggcatatt cttatcctta aaattaacca tgttatcgat
    11041 aaggctatat atgaccttaa tttgcatcta atatcaaagg gattataacg atatggcacg
    11101 agttaacctg ttgacattca ggtgttttaa aggtagcagc tcatggtgtg ttatccagta
    11161 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
    11221 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
    11281 tagttttata gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
    11341 agtaatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
    11401 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
    11461 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
    11521 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
    11581 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
    11641 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
    11701 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
    11761 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
    11821 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
    11881 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
    11941 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
    12001 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
    12061 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
    12121 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
    12181 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
    12241 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
    12301 ctgcgctcct ggtctaatca aatagtcgat ggtgatatca gcacctgcgt cagcgtggcg
    12361 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
    12421 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
    12481 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
    12541 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
    12601 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
    12661 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
    12721 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
    12781 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
    12841 gacaccttgg aactttgtta tgatgtggcg aaactgtcac tggggcgttc cgccttgctg
    12901 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
    12961 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
    13021 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
    13081 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
    13141 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
    13201 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
    13261 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
    13321 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
    13381 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
    13441 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
    13501 tcattggtaa ttatcgacaa attgccgttt acctcgccgg atgatccact gttaaaagcg
    13561 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
    13621 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
    13681 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
    13741 agtctgccgc ccgcgccacg cacccgtgac attgcccgtg cggttcgttt ccttgcgata
    13801 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
    13861 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
    13921 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
    13981 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
    14041 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
    14101 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
    14161 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgat
    14221 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
    14281 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
    14341 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
    14401 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
    14461 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
    14521 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
    14581 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
    14641 ctggcaggta cgtttgcgct aatgctgagc ggttgtgtca ctgtgccgga cgccattaaa
    14701 ggcagcagta ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
    14761 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
    14821 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
    14881 ccttctcgcg gtcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
    14941 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
    15001 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
    15061 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
    15121 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
    15181 accgaataat tgcttgtttt taaagaatgc agaaacagcg gctggtccgc tgtttctgca
    15241 ttcttacggt aaagataaaa ataaatagtg acgcgcttcg caaccttttc gttgggtaat
    15301 tatcaagctg gtatgatgag ttaatattat gttaacggcg tgtatatcat ttggggttgc
    15361 gatgacgacg aacacgcatt ttagaggtga agaattgaag aaggtttggc ttaaccgtta
    15421 tcccgcggac gttccgacgg agatcaaccc tgaccgttat caatctctgg tagatatgtt
    15481 tgagcagtcg gtcgcgcgct acgccgatca gcctgcgttt gtgaatatgg gggaggtgat
    15541 gaccttccgc aagctggaag aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt
    15601 ggggctgaag aaaggcgatc gcgttgcgtt gatgatgcct aacttattgc aatatccggt
    15661 ggcgctattt ggcattttgc gtgccgggat aatcgtcgta aacgttaacc cgttgtatac
    15721 cccgcgtgag cttgagcatc agcttaacga tagcggcgca tcggcgattg ttatcgtgtc
    15781 taactttgcc cacactctgg aaaaagtggt tgataaaacc gccgttcagc acgtaattct
    15841 gacccgtatg ggcgatcagc tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa
    15901 atacatcaag cgcttggtgc cgaaatacca tctgccagat gccatttcat ttcgtagcgc
    15961 actgcataac ggctaccgga tgcagtacgt caaacccgaa ctggtgccgg aagatttagc
    16021 cttcctgcaa tacaccggcg gcaccacagg tgtggcgaaa ggcgcgatgc tgactcaccg
    16081 caatatgctg gcgaacctgg aacaggtcaa cgccacctat gggccgttgc tgcatccagg
    16141 taaagaactg gtagtaacgg cactgccgct gtatcacatt tttgccctga ccattaactg
    16201 cctgctgttt atcgaactgg gtgggcagaa cctgcttatc actaacccgc gtgatattcc
    16261 agggttggta aaagagttag cgaaatatcc gtttaccgct atcactggcg ttaacacctt
    16321 gttcaatgcg ttgctgaaca ataaagagtt ccagcagctg gatttctcca gtctgcatct
    16381 ttccgcaggc ggtgggatgc ctgttcagca agtggtggca gaacgctggg tgaaactgac
    16441 cggacagtat ctgctggaag gttatggcct gaccgagtgc gcgccgctgg tgagcgttaa
    16501 cccgtatgat attgattatc atagcggtag catcggtttg ccggtgccat cgacggaagc
    16561 caaactggtg gatgacgacg acaacgaagt accaccgggt caaccgggtg agctttgtgt
    16621 caaaggaccg caggtgatgc tgggttactg gcagcgtccg gatgctacag atgagatcat
    16681 caaaaatggc tggttacaca ccggcgacat cgcggtgatg gatgaagaag gattcctgcg
    16741 cattgtcgat cgtaaaaaag acatgatttt ggtttccggt tttaacgtct atcccaacga
    16801 gattgaagat gtcgtcatgc agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc
    16861 ttccggctcc agtggtgaag cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac
    16921 cgaagagtca ctggtgactt tttgccgccg tcagctcacg ggatacaaag taccgaagct
    16981 ggtggagttt cgtgatgagt taccgaaatc taacgtcgga aaaattttgc gacgagaatt
    17041 acgtgacgaa gcgcgcggca aagtggacaa taaagcctga gcgttaagtc agtcgtcaga
    17101 cgccggttaa tccggcgttt tttttgacgc ccactaaaga gaaaacaatt tgaattacca
    17161 aatgattacc acggacgatg cgctggcttc tttgtgtgaa gccgtccgtg tctttccggc
    17221 gatagccctg gatactgaat ttgttcgtac gcgcacttat tacccgcagc tggggttgat
    17281 tcaacttttc gatggcgagc atctggcgct aattgatcct ctcgggatca ccgactggtc
    17341 accgctgaaa gcgatcctgc gcgatccgtc catcacaaaa tttctccatg caggcagtga
    17401 agatctggaa gtgttcctca atgtctttgg cgaattacca caacccttga ttgacacgca
    17461 aatccttgct gccttctgcg gacgcccgat gtcatggggt ttcgcttcca tggtggaaga
    17521 gtattccggc gttacgctgg acaagagtga atcgcgcacc gactggctgg ccagaccgct
    17581 gaccgaacgt cagtgtgaat acgcagcggc ggatgtctgg tatctgttac cgatcaccgc
    17641 caagcttatg gtagaaacgg aggcctccgg ctggctacct gcggcgctgg atgaatgccg
    17701 cctgatgcaa atgcgtcgtc aggaagtcgt tgcgccggaa gatgcctggc gtgatatcac
    17761 caatgcctgg cagttacgca cacgccaact ggcctgtctg caactgttag ccgactggcg
    17821 actgcgcaag gcgcgagagc gtgatctggc ggtgaacttt gtcgtgcgtg aagagcattt
    17881 gtggtcggtc gcgcgttata tgccgggaag tttaggcgaa ctggacagcc tgggtttatc
    17941 cggtagcgaa atccgctttc acggtaaaac gctgctggcg ctggtggaaa aagcgcagac
    18001 attgccggaa gatgccttac cgcagccgat gcttaacctg atggacatgc ctggttatcg
    18061 taaagcgttt aaagcgatta agtcgctgat tactgacgtg agcgaaacgc ataagatcag
    18121 cgccgaattg ctggcatcgc gtcggcaaat caaccaactg ctgaactggc actggaaact
    18181 gaaaccgcag aacaatttgc cggagctgat ttccggctgg cgtggtgagc tgatggcgga
    18241 agcattacac aatttattgc aggaatatcc gcagtaaaat cttccgaagc cggactgggc
    18301 gcgctcagcg ccacatccgg ctttggcaaa ctacaaatcc aacaccaggc gtttaccctt
    18361 cgcacgcgaa caacagatca acatactttg ctggctggca cgctcttcat cgctaaaata
    18421 ctgatcccga tggtcagctt caccttccag tattgccgtt tcgcaggttc cgcatacccc
    18481 ttcacgacat aaacattcca ctttcgcggc tttattattt tcaataacct gcaaaatagt
    18541 catctcttcc ggcaccacaa actcttttcc ggaacgggca agcaccaggg taaatgcatc
    18601 gccggttttg tcttcgatag caaattgctc aaagtgcagt gtatcggcgg cgatgtccag
    18661 acgcgcagct tcacttctta ccgcttcaat tagcgcctcg gggccacagg tataaacgtg
    18721 tgtgccaggt tcgatatccg ccaataatcg cgctaattcc agtcgtgttc cggttgatga
    18781 atgatgcaaa tggactttct cagcctgcgg atgctggact agctcagcac gaaatgcgca
    18841 actttctgga tttcgcgagc agtaatgtag ctgccagtcg atgtcgctgt gttgcaactc
    18901 tgccatgtgc gacaggaaag gggtgatacc aataccgccc gcgataaaca gatgctttct
    18961 ggctgaggga attagcgcaa acaggttatt aggcgttgag atcgttaacc gatcgcccac
    19021 ttttacctgc tgatgcaaaa agcgggaacc gccgcgcgag ttttcttcca gtcgaacggc
    19081 aatctgataa ctagaggtgt tatgcggtga actcagtagt gaatacgcat tgctgtactg
    19141 gttatcacca tcgctcatct gcactaggat gtgacttcct ccggtgaacg caggcagcgg
    19201 tttgccatcg gttgccacca gcgtgaagcg tttcacctgt tcggtaaggg gttcaacctg
    19261 gctcacctgt acttcaaaca tttgatagtc tgacattgct gtactccatc aaaaatcagc
    19321 ccagcggcgg aaaataccgc cgccgatgtc attagtcctt aaacacctgc gccagcagat
    19381 tatggaaatg ggcgatacca tgttcggaaa tgccactacc gctactgtcg gccatgatgc
    19441 gcccctgacc acgatagcca cgcgatttca gccctttctg tacgctttca accagacgta
    19501 aatcttccgg atggaacaca tcgcgatacc actcaatcag cgatttttgc tcgtcggtta
    19561 actcttcatt ggtgaagtaa atatcgtagt tttgcagggt agtttcagaa tccaccggga
    19621 attcataaat gaccgtcatc atccctttga tcggggtgac gttcagcatc gtgcacggcc
    19681 acagccagaa accgtggaat gccgcatccg taccctcttc aaatttaaac gactgttcgg
    19741 acggtttggc gaaaccgtat tgcagcgtcc agttaccgtg catggtgtgc cagtaacgat
    19801 caacctgtac ggagtcggag aaacctagat gcgccggacc acagtgatag cactcgagat
    19861 agttatcgac aatgttcttc cagttggcag gcgtgcgggt ggtaaagcgg gccgctagtt
    19921 tcagattgtg gacttccggg caggcttcca gcactttcgc gcccagaccg gtgtattgat
    19981 tcacttgaag tacgaaaaaa aaccgggagg acattggatt attcgggttc tgatgggatt
    20041 agatttggtg gggcttgcaa gcctgtagcg caaattttag tcgttaatca atgaaacgcg
    20101 aaagatagta aaaaattgct tttgtttcat tgaaaatacg aaaaacaaaa acactgcaaa
    20161 tcatttcaat aacagcttca aaaaacgttc aaaaccgata acaaccaggc tgtaaccaaa
    20221 tgactcatat cacaaatcag ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag
    20281 gtgaagtatg ttttagctgg ttcatggttg ttatacggct ttttttacat cttgtggttc
    20341 ttgtgaagga gctacaagac cttcctgttc ataaatacca tactttgaca aaatcttttt
    20401 acgagtatct gttggtaatg attcaagcat tttggctgtt tgtaaggttt gctgcgaaat
    20461 accttgcaaa tcagcctgga tggaatcatc ggaagaaaaa atctcaggca taagagcttc
    20521 aaatcctttc tttctgaaca actcaatcgc agaatgcagt tccttgtcct ctaacaattc
    20581 gacaattgcg atcagtttag ttttcaatgt gctgcggtca ggttgagaat ttttattctc
    20641 ttgattattt ttatgctcaa ccattggcat gctctgactt tggataaacc actctattgg
    20701 acgccctgtt gcgtaggcta tttgtgctaa tcgatctaaa gacgggtatg tcttgccaga
    20761 caggtagttt ctgataaccc catcagaaag tccgcactta gctgcaaacg cccggcttga
    20821 ttctccattc attgcctctt caaggcgttc cttgaaacgc attatttccg ggggcataaa
    20881 aatgcgctct gcatttttgc tttctttttc acctgtcatg cccaatctct ctgttatata
    20941 aagaatttta cagaattctt tgatttgcac tggttaaaat aaaaatgcgc atagcttttg
    21001 aattgagcat ttttctgcgc tatcgttttt cccgatagcg gtagccggta tcagttaccg
    21061 aaaacgttaa gagagtaagg atcgcagaat gtcagcaaaa aaccaagact ggcacgcaga
    21121 gcagataaag gctgctgttc gtatgaaggg gaagacacta tctcaattat ccagag
//
LOCUS       DAJTPG010000067        21123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTPG010000067 DAJTPG010000000
VERSION     DAJTPG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(126..554)
                     /gene="mxiM"
                     /locus_tag="OO230_002584"
     CDS             complement(126..554)
                     /gene="mxiM"
                     /locus_tag="OO230_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5692292.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(538..945)
                     /gene="mxiM"
                     /locus_tag="OO230_002585"
     CDS             complement(538..945)
                     /gene="mxiM"
                     /locus_tag="OO230_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5692293.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(938..1633)
                     /gene="mxiN"
                     /locus_tag="OO230_002586"
     CDS             complement(938..1633)
                     /gene="mxiN"
                     /locus_tag="OO230_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5692294.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(1575..2102)
                     /locus_tag="OO230_002587"
     CDS             complement(1575..2102)
                     /locus_tag="OO230_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5692295.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(2099..2824)
                     /gene="mxiJ"
                     /locus_tag="OO230_002588"
     CDS             complement(2099..2824)
                     /gene="mxiJ"
                     /locus_tag="OO230_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5692296.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(2830..3123)
                     /gene="mxiI"
                     /locus_tag="OO230_002589"
     CDS             complement(2830..3123)
                     /gene="mxiI"
                     /locus_tag="OO230_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5692297.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(3136..3387)
                     /gene="mxiH"
                     /locus_tag="OO230_002590"
     CDS             complement(3136..3387)
                     /gene="mxiH"
                     /locus_tag="OO230_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5692298.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(3395..4510)
                     /gene="mxiG"
                     /locus_tag="OO230_002591"
     CDS             complement(3395..4510)
                     /gene="mxiG"
                     /locus_tag="OO230_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5692299.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(4519..4977)
                     /gene="ipgF"
                     /locus_tag="OO230_002592"
     CDS             complement(4519..4977)
                     /gene="ipgF"
                     /locus_tag="OO230_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5692300.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(4977..5339)
                     /gene="ipgE"
                     /locus_tag="OO230_002593"
     CDS             complement(4977..5339)
                     /gene="ipgE"
                     /locus_tag="OO230_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5692301.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(5349..6965)
                     /locus_tag="OO230_002594"
     CDS             complement(5349..6965)
                     /locus_tag="OO230_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5692302.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            7279..8763
                     /gene="icsB"
                     /locus_tag="OO230_002595"
     CDS             7279..8763
                     /gene="icsB"
                     /locus_tag="OO230_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5692303.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            8776..9165
                     /gene="ipgA"
                     /locus_tag="OO230_002596"
     CDS             8776..9165
                     /gene="ipgA"
                     /locus_tag="OO230_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5692304.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            9181..9807
                     /gene="ipgB1"
                     /locus_tag="OO230_002597"
     CDS             9181..9807
                     /gene="ipgB1"
                     /locus_tag="OO230_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5692305.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            9864..10331
                     /gene="ipgC"
                     /locus_tag="OO230_002598"
     CDS             9864..10331
                     /gene="ipgC"
                     /locus_tag="OO230_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5692306.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            10337..12079
                     /gene="ipaB"
                     /locus_tag="OO230_002599"
     CDS             10337..12079
                     /gene="ipaB"
                     /locus_tag="OO230_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5692307.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            12099..13190
                     /gene="ipaC"
                     /locus_tag="OO230_002600"
     CDS             12099..13190
                     /gene="ipaC"
                     /locus_tag="OO230_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5692308.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            13241..14239
                     /gene="ipaD"
                     /locus_tag="OO230_002601"
     CDS             13241..14239
                     /gene="ipaD"
                     /locus_tag="OO230_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5692309.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            14248..16149
                     /gene="ipaA"
                     /locus_tag="OO230_002602"
     CDS             14248..16149
                     /gene="ipaA"
                     /locus_tag="OO230_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5692310.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            16153..16389
                     /locus_tag="OO230_002603"
     CDS             16153..16389
                     /locus_tag="OO230_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5692311.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            16606..17535
                     /gene="virB"
                     /locus_tag="OO230_002604"
     CDS             16606..17535
                     /gene="virB"
                     /locus_tag="OO230_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5692312.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(18479..19258)
                     /gene="ipaJ"
                     /locus_tag="OO230_002605"
     CDS             complement(18479..19258)
                     /gene="ipaJ"
                     /locus_tag="OO230_002605"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5692313.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(19858..19995)
                     /locus_tag="OO230_002606"
     CDS             complement(19858..19995)
                     /locus_tag="OO230_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692314.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            20611..>21123
                     /gene="istA"
                     /locus_tag="OO230_002607"
     CDS             20611..>21123
                     /gene="istA"
                     /locus_tag="OO230_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR5692315.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNA"
BASE COUNT         7184 a         4333 c         3172 g         6434 t
ORIGIN      
        1 gagccagctc aattatgtta cgttctttat cttttacaag ctcaacgtta actggttcat
       61 gtccactaga gtaaatgtaa aacatattac ttgtatttag acctttatat ttactcacaa
      121 tacaattata tccctcgaat taattttaat ttaacttccc catccttctc tataaggatg
      181 gtatcattta tcttattacc ttttaaaaca accccgtctt tcagcataga aaatagttta
      241 tctatagcta tcttttgtgc atccatgaag cacccatcta cttccttaac attaaagttg
      301 cccatgaatt tacctgcatt aaaactataa accgccttac cactaataca tttagagtaa
      361 acatttatac ttttattggt tgtattaaac gaccataata aatcatttgg aatagaaaaa
      421 taatccttac ttacaggaac aatatgccat tctttttcag aattagatga tgactttaaa
      481 gcacacccac tcagtgttaa tagcaatata cttaaaataa atattttcaa cttattacta
      541 ccatgtcgaa tcatctgcct ctctccctgc ttcaaagaca ctaattaatg aatcgaaggt
      601 tgtagtctta atatagaaat caatattgtt atatatggac aatgatgcat aaaatccctc
      661 atgcaaatcg acatattgat ttgcatctat gagtttatta agaagtgaac ttttttgtgc
      721 agctgtaaga taaggaaaac gtgaattgaa taattgttca atcttattgt gcagttcatc
      781 attatctatg gaatttaatt cactctgtga gaaatattgc attatttcat agattatatc
      841 ctcatcaaat gattgcgaag atgataatct ctcatttaaa ttaacaactt cttcactatt
      901 taatctttgc tgcagagcat tgcttgcatt tatttgatta atcattaaca ggattctctt
      961 ttaacaagtc ttctgcaatt tgtctcagtt tcttatgtgc taactctttg aaatattttt
     1021 tatcattttt tgttaatttt tccgcaagtt ctattttaac acctgatatt acatcctgag
     1081 gactaaactc cactacttga ttaccactac agaatatata attattccct tcagaatatt
     1141 ttaaaataat cttgacatct gagcgatatt tatggagatc aagttctaac tttttcctca
     1201 gtgctaaatt acacttgggc aaaaccactt ttaattctgt tactgtatta cgcaacgaac
     1261 taagccactg ctccaataaa gtgctaatga tcctctcatc atgtaaattt tctgaaagca
     1321 aactagttat atagtcctca atatttcttc tattttcatt ttgctttaac ttccattcac
     1381 aaatatagtc aataatatgc tcaaaagcag tctgtatacc aatttgataa ccatcacata
     1441 cagcatctat acgcagtgat tcagcttttt tcgtagcctc ctttataaca caaattgctt
     1501 tcttcttact ctcagtgtct ctatctttta tggttttaca taattccttt tcaattctct
     1561 ttataacaac accatcatag gcatgatgtc tggataccgg taaagtgcct ttttgcatat
     1621 tgcatacctt catttaatcg ggacaaattt ctttctacag gcgtataatt tccctccaaa
     1681 gcaattggat taaataataa gtttattctt tcatatatac caacagatat attttccttc
     1741 cacgcaataa gatgccaaaa accacttcga atagcggcct ccccttgctt actgattttg
     1801 cccccataat aactaataac catctcacta tgattaatta accgagactc ttgactaccg
     1861 ataaaataag caacagctgg aagtaaattc cagttggcaa caaacatagc ggttaccccg
     1921 cttaaaggtt caatatcgca atttagatca tatttattaa ttatgagatt attaatctca
     1981 cttctgatta cgccattttc taacagttca gaaggcaaat tcaaccgatt tctatttata
     2041 tagaacgctg gatcaaaaat tattgaaaga tattttttat aaattccatc cattcttatc
     2101 atattttgtt tctcttgaac caccctgttt tgaatgccca taccaaaaga atgacaacta
     2161 gtacagccat cccgagaaat aaatatatta cttcatttgt taaaaattcc gatttaactt
     2221 ccttaacagg ttgtacattt gtataaacat attcttcttt cggagttaat atgacagata
     2281 tattttcata cttaacatca gaaaaggtgt ttttcaaaaa tcgcttaata ttactaacta
     2341 atagttcaga ctctttcggt gagtcatata tagcgattac tgatatatgc atcggttttg
     2401 aagatatatt tttttcttca agatcatagc taacatgtat ttttgccgaa ataacaccac
     2461 caatagaaac taaagactgt tccagccgtt gctcaatagc actatataaa cgggcctttt
     2521 cagctcttgg agaagacact aatgaatctg taggaaacat ttgtgagata tcaactctct
     2581 ccggatttgg caaatcgtac atgcgcatca aatcaactgc cgatgcaaat gtcccctttt
     2641 cgacttgtac cgagatcccc tgtttacctc catcaacttt tctagcagta atattatggc
     2701 gttctagcac agatattatt tcatttgcct gtctttgaga taaattagaa attaactctt
     2761 cacgttgctc acatccaatc aacatcagca acaagaataa aataaaacct ttatacctaa
     2821 tcatataaat taagacttta ataaagtttc aacagccgaa actgtttttc ttgctaacgt
     2881 gccagctaat gatactccta ttgaataatt tgagagcgtc gtctgtaact tggccaaaga
     2941 ttctgggttt aatgattctg gattgcttac catctccatt atttgtgata cttgaatatc
     3001 tgtatccatc ttaatatcac tatatttagc cgacacgacg tcttccagac ttgatatctc
     3061 ttgagattga aaatcactgg ctttgataat atcaacctga ttgactggat aaatgtaatt
     3121 catgaatgct ccctattatc tgaagttttg aataattgca gcatcaacat ccttaatcac
     3181 tttcactgta ttggattgcg cgttcctata taatgtatat tcagataatt tactttggta
     3241 ttcagccagc aactgtggat tcgaaggatt tttagctaat ttatctagtg ccaatgttag
     3301 ttcaccttgt aatgtttgag ttccatcatc aaaagtttca gataatgaac tcaatgtcca
     3361 atcatcattc ggtactgtaa cactcatttt atcctcactt atttttatct aaaaaaaacc
     3421 agtgtttatc attcaacatt acataactgt ctttgctgtt aagatatgat ttacctttaa
     3481 aatcatcatc tatcaaaagt acagaaaact caatatactg atctccatat atatttttat
     3541 gttcagaaat cagtctatta atgagctcaa gcttttcatc tgtcagttct tctctgacag
     3601 aatatgtaac tttattattt tcacaaatct ctttatactg gacatttacc tttgtgaaaa
     3661 tacctttagc atcccccctt gcattatgat cgctaagata gttgaattta atatttttga
     3721 tatacggaaa ttcaacaatc agagcatttt tcaatttatc aagttcggta tcttttgaac
     3781 tatttctttc tttacttaaa aacaccagta attccgcttt atcactcact aaattcaaca
     3841 catacaaatt gataaatggg aatcgttgat tgatatattg ttgaattcta tttatttctt
     3901 tgtttgacac cagaatatat ctgcatttgg taaatccggt tttatttaag gctacagacg
     3961 cccataccgc atcattttgt gtttcggcaa gaatataatt gcactgtcca gtgttgacta
     4021 ttccatatct cttatcatct ataatttcag caatttcttt tacttcatct ttctttaaag
     4081 agagggaaaa aattataatt aacacaacaa taacagcaaa gaaaaaaaac accgagtggt
     4141 tcttatacat tccgtttagt atatggcctc tcgatttatc ctccctcata tttttaaggt
     4201 gaaaagaaat cccgtcatat tcaaatgaat taaattgtga attgatatca atttgctcac
     4261 ctttttcatc actaaattga cagatattat ctttgcttat ggtcaaataa attataccat
     4321 ccgatttact atctgttaca ggaataatat tttctgggaa attgtcgttt ccaaactcaa
     4381 gtgtttctat cgtccgtccg agaacaatta agttattccc ataatataac ggaaactcat
     4441 ccccgacacc gtttgtgagt ttcactaata acctgaatgg tgcaagattt gagttctttg
     4501 cctcagacat aaatcctctt atatccttcg attattctgc ttgctctctg ctgctatccg
     4561 caaatagcgt ctatagatat cctctgcgta ctttaatctt tcttttttct ttttcggaga
     4621 cgttcctgca ttataggctc caacagcctc ccaccctctt ccatacatca tcataaactc
     4681 atttaataac tttgctgcat aatgtacaga aagacatgga tgactaatta gcatttcctc
     4741 agaatatccc atttctctaa gtcttttaga atgaaagtca tttatttgca ttataccata
     4801 atctttactt ccattgttat taacattgac tgcagattta ttaaatccgg attctttttc
     4861 cgcaatcgct tttaacaaac ttgatggaat attgtacctt tcaccagcct tatcccaaca
     4921 atcagctctc cctaaatatg ggataaaaca cagcaaaata aatacgaaac gggacattaa
     4981 taccccttca ttcttcgcgc aaattcatcc ttcagccaac gcacacgcga aatataatat
     5041 tctgtattaa catatacatc ctcgaattca tttatcccgg taagatctaa tcgcgcaatt
     5101 agatttccgc cttcatcatc agtagctaag catatctttt ctgagtaatt taggctcaac
     5161 gcatatataa gatcatttat attttcaggc aatgcacaaa aagggcataa taaatttatt
     5221 gaatctcctt ctttttcaat atatattagc acatcatcat caagcatgat tgcttgatca
     5281 tcgatatcaa ttaaagggat acccaaggct cggcaaataa catctgctaa atcttccata
     5341 tattcctctt atacaaatga cgaataccct ttcaccatat tccatatttt tgggtcccct
     5401 attctttcag aataagatag ctctaacgaa gatagcggca attttttcat gactttattt
     5461 ccgggcacac cagtattcat ctcttggatt tccatattac cactattcat tagaatagta
     5521 gaaaataatc ttttctcttc tgaggataat ttactattta attgagaaaa ctgacctgtt
     5581 tcatgttttc ttattatttc tcttttaatt tcagcatcct gcatacctgt tctgtccttc
     5641 ccactcttac aattccagca aggtacagca cctatagtat aagccaatag agtcattctt
     5701 tgtgacaatt tatacggctc tccattatca tttttttgta actttttggt tacaatctct
     5761 tttatctggt tagccagata tatcacatca ttcttacatg gtggattttt ttctattgct
     5821 tctgcagccc aacctccaat cacaccattt ttgaggaaat tatcccccaa caaagaacat
     5881 attgattcat cattaagctt gtcaacattc ctccagccta agcccatttt tagcgctaat
     5941 tcatttacac caaagttaaa tgtcaccact tttagattaa cgcttacttc tttcagaaga
     6001 ccatcactat tccgaattaa taatttcgtt ggcttccccc ttttagagtt taaccctttt
     6061 aatgcgttca cctgatcttt taacatactc tcctctcccc cggttaaact ggtcggcgtc
     6121 agaagagaag tcgaaacaat ctttaaatct actgttttac cagacaaagc ctgtgataat
     6181 aactctggtc tgctatataa tgctgcgctt actaactcct cagctttatt acgagcagca
     6241 acagctcttt cagatgagtt ttttttcaat ccataggctg atataactcc atgtctgata
     6301 cccgaaaaaa tttccttccc ctcatcatcc accacttttg aaagccacat attcgcgata
     6361 tgatcactct ctctcgtaga agcacaacaa attccttttc cattatactc tttgacaaat
     6421 atatttttat tccctatctt catatgagag gcaggagtta attgaaaacc ataatttttc
     6481 ccatgatgac taatattctt gtcgaccggt ccccaattct tattatttaa ataatcagta
     6541 aatatttgtt ttattttcgt ttgcaattcc tttttatttt taatgcctag ctgtgctctt
     6601 atctcttcac ttattacttt attaagctca ttaatatgtc ttttattaac ttcaccaagt
     6661 ttataggtat ctaatcgttc catcatggct ttaatttttg acgtatcacc acctgccaac
     6721 tttattgcct cgcaaaaaac tccagctctg tcacgcgcaa acaaaaggct ttttcctgtt
     6781 aagcttgttt ggttctgtaa atataatcga ttaagcgctt cgatcccctt atttctttct
     6841 ccatttttaa ctctttgata tgaaatcacg cttacacctt tgtgctttcc ggtttcttct
     6901 gcgcctttat aggaatctcc gctttgaaat gaaacctgat gcaatcccaa attagttatg
     6961 tgcatttaat tatcctcagt cataaatggg gcattgattg cagttttgta cctcgtttta
     7021 caacaaaaaa gatacttatt caacagggtt ctctcttttt ctataacact ccgagcctga
     7081 tacatcaggc actttatctt gtgggatttc atgatgaaac gagcactaca tatgctcacg
     7141 aggtacatat tatgtacagc aaaagaaaat ttgattatgt taacaggctc aagtttctaa
     7201 atttattaat tgaatatatt atataccaaa tacattttac atccccacaa tcaccaagta
     7261 atggagagtt aataaagtat gatcctcaaa attagcaatt tcattgacgc aagcaataca
     7321 aaagggccta tacgcgttga agatacagag catggaccta tattgatagc acagaaattc
     7381 aacctaaagg atctcttttt cagaacatta agcactatta atgcaaagat aaattcacag
     7441 atattaaatg aacaactaaa aaattatcga ctagagaatc aaaaatcctt attgcttttc
     7501 ctcaatacat tagctagcga aaaatctgca gaaagtgcgt ttgccgccta tgaagcagct
     7561 aaaaactcaa ttcaacactc tttcacaggt agagacatca aattaatgtt gaataccgca
     7621 gagcgttttc atggcatcgg tacagccaaa aatcttgaaa gacatttagt ttttcgttgc
     7681 tggggaaata gaggcataac ccatttaggg catactagta tcagtataaa aaacaattta
     7741 ctacaggaac caactcatac ctatctcagc tggtatcccg gcgggaatgt tacaaaagat
     7801 acagaaataa actacctttt tgaaaaacgc tcaggttaca gtgtcgatac ctataagcaa
     7861 gacaaactaa atatgatttc agaccaaaca gccgaaagac ttgatgcagg ccaagaggtt
     7921 agaaacttat taaattcaaa acaagatcaa aataacaaca aaaaaatatt ttttcccaga
     7981 gctaatcaaa aaaaagaccc ctatggttat tggggggtca gtgctgataa agtatacatt
     8041 ccattatcag gtgataataa aacaaaggat ggtaaaatca gccataacct ttttggcctt
     8101 gatgaaacaa acatgtcaaa atttatatgc aaaaagaaag cagatgcttt cagacaattg
     8161 gcaaattata aattaatcag taaatctgaa aactgtgctg gtatggcact taatgttctt
     8221 aaagcaggaa attctgaaat atactttcca ctccctgacg ttaaattggt tgctacacct
     8281 aacgatgtat acgcgtatgc aaataaagtt aggcaacgca ttgaaagtct caatcagtct
     8341 tataacgaaa taatgaagta tattgaatct gattttgatc tttccagatt gactcaatta
     8401 cgacgcagct atcttaaaag tttcaataaa attaacctta tccacacacc aaaaacattc
     8461 aagcctttat caatttcact ttacaaacat cctactgaaa atgtatcttc agaagacttt
     8521 gatgcggtca tcaacgcttg ccactcatac ttagtaaaat cagcacccag caatatgact
     8581 cgtgtattaa acgaattaaa aaccgaagca acagataaaa aagaagaaat catcgaaaaa
     8641 tcaataaaaa ttattgatta ttacaattct cttaaatcgc cagatcttgg aaccaaactt
     8701 tacattcacg acttattaca gatcaataag cttttattga ataactctca ttctaatata
     8761 tagaaggcca tagaaatgtg tcgcaaacta tatgataaac tttatgaaat aacaggtgca
     8821 aagcttgatt ttaacgataa aaatcaagcc tttatattgc tcgaagaaca aatacctgtt
     8881 tgtataacag ataatgatga atacatcttt ttgaccggac ttcttaatga acatgaactt
     8941 tttacagaaa atattattaa tccagaacac atactcatcc ttaactactc tttgagtaga
     9001 gattatggtt cttctatctg tcttctccct gatactcatc aatgtgtttt gaccaagaaa
     9061 cactacaaaa aatatctttc acctgatgaa ctcattgaat ctttgtatga atttttattt
     9121 tgtattaaac taaccattgc aaacatcact tcagaagtga actaacatat agggggtatc
     9181 atgcaaattc taaacaaaat acttccacag gttgaattcg cgattcctcg cccatcattt
     9241 gattcgttaa gtcgcaataa gcttgtaaag aaaatattat ctgtttttaa cttaaaacaa
     9301 cgctttcctc agaagaattt tggatgcccg gtgaatataa ataaaatcag ggacagcgtc
     9361 atagacaaaa taaaagatag taattcgggg aatcaactat tttgttggat gagccaagag
     9421 cgaaccactt atgtctcttc aatgataaac agaagtatag atgaaatggc catacacaat
     9481 ggagttgttt tgacctcaga taacaaaaaa aacatatttg cagctattga aaaaaaattc
     9541 ccggatataa aacttgatga aaaatcagca caaacatcta tcagtcatac agcactaaac
     9601 gagattgcct catctggcct cagagcaaag attttaaaac gctatagcag tgacatggat
     9661 ttgtttaaca cacaaatgaa ggatcttaca aatctagtat ccagttccgt ctacgataaa
     9721 atatttaatg aatcaacaaa agttttacaa atagaaataa gcgcagaggt tctaaaggct
     9781 gtataccgtc aaagcaatac aaattaaatc ttttatatta aatcttatac ttattaaagt
     9841 aaagcagaca aaaaggagac cttatgtctt taaatatcac cgaaaatgaa agcatctcta
     9901 ctgcagtaat tgatgcaatt aactctggcg ctacactgaa agatattaat gcaattcctg
     9961 atgatatgat ggatgacatt tattcatatg cttatgactt ttacaacaaa ggaagaatag
    10021 aggaagctga agttttcttc aggtttttat gtatatacga cttttacaat gtagactaca
    10081 ttatgggact cgcagctatt tatcagataa aagaacagtt ccaacaagca gcagaccttt
    10141 atgctgtcgc ttttgcatta ggaaaaaatg actatacacc agtattccat actggacaat
    10201 gccagcttcg gttgaaagcc cccttaaaag ctaaagagtg cttcgaactc gtaattcaac
    10261 acagcaatga tgaaaaatta aaaataaaag cacaatcata cttggacgca attcaggata
    10321 tcaaggagta attattatgc ataatgtaag caccacaacc actggttttc ctcttgccaa
    10381 aatattggct tccactgagc ttggagacaa tactatccaa gctgcaaatg atgcagctaa
    10441 caaattattt tctcttacaa ttgctgatct tactgctaac caaaatatta atacaactaa
    10501 tgcacactca acttcaaata tattaatccc tgaacttaaa gcaccaaagt cattaaatgc
    10561 aagttcccaa ctaacgcttt taattggaaa ccttattcaa atactcggtg aaaaatcttt
    10621 aactgcatta acaaataaaa ttactgcttg gaagtcccag caacaggcaa gacagcaaaa
    10681 aaacctagaa ttctccgata aaattaacac tcttctatct gaaactgaag gactaaccag
    10741 agactatgaa aaacaaatta ataaactaaa aaacgcagat tctaaaataa aagacctaga
    10801 aaataaaatt aaccaaattc aaacaagatt atccgaactc gacccagagt caccagaaaa
    10861 gaaaaaatta agccgggaag aaatacaact cactatcaaa aaagacgcag cagttaaaga
    10921 caggacattg attgagcaga aaaccctgtc aattcatagc aaacttacag ataaatcaat
    10981 gcaactcgaa aaagaaatag actctttttc tgcattttca aacacagcat ctgctgaaca
    11041 gctatcaacc cagcagaaat cattaaccgg acttgccagt gttactcaat tgatggcaac
    11101 ctttattcaa ctagttggaa aaaataatga agaatcttta aaaaatgatc tggctctatt
    11161 ccagtctctc caagaatcaa gaaaaactga aatggagaga aaatctgatg agtatgctgc
    11221 tgaagtacgt aaagcagaag aactcaacag agtaatgggt tgtgttggga aaatacttgg
    11281 ggcactttta actatcgtta gtgttgttgc agcagctttt tctggaggag cctctctagc
    11341 actggcagct gttggtttag ctcttatggt tacggatgct atagtacaag cagcgaccgg
    11401 caattccttc atggaacaag ccctgaatcc gatcatgaaa gcagtcattg aacccttaat
    11461 caaactcctt tcagatgcat ttacaaaaat gctcgaaggc ttgggcgtcg actcgaaaaa
    11521 agccaaaatg attggctcta ttctgggggc aatcgcaggc gctcttgtcc tagttgcagc
    11581 agtcgttctc gtagccactg ttggtaaaca ggcagcagca aaacttgcag aaaatattgg
    11641 caaaataata ggtaaaaccc tcacagacct tataccaaag tttctcaaga atttttcttc
    11701 tcaactggac gatttaatca ctaatgctgt tgccagatta aataaatttc ttggtgcagc
    11761 gggtgatgaa gtaatatcca aacaaattat ttccacccat ttaaaccaag cagttttatt
    11821 aggagaaagt gttaactctg ccacacaagc gggaggaagt gtcgcttctg ctgttttcca
    11881 gaacagcgcg tcgacaaatc tagcagacct gacattatcg aaatatcaag ttgaacaact
    11941 gtcaaaatat atcagtgaag caatagaaaa attcggccaa ttgcaggaag taattgcaga
    12001 tctattagcc tcaatgtcca actctcaggc taatagaact gatgttgcaa aagcaatttt
    12061 gcaacaaact actgcttgat acaaataagg agaatgttat ggaaattcaa aacacaaaac
    12121 caacccagat tttatataca gatatatcca caaaacaaac tcaaagttct tccgaaacac
    12181 aaaaatcaca aaattatcag cagattgcag cgcatattcc acttaatgtc ggtaaaaatc
    12241 ccgtattaac aaccacatta aatgatgatc aacttttaaa gttatcagag caggttcagc
    12301 atgattcaga aatcattgct cgccttactg acaaaaagat gaaagatctt tcagagatga
    12361 gtcacaccct tactccagag aacactctgg atatttccag tctttcttct aatgctgttt
    12421 ctttaattat tagtgtagcc gttctacttt ctgctctccg cactgcagaa actaaattgg
    12481 gctctcaatt gtcattgatt gcgttcgatg ctacaaaatc agctgcagag aacattgttc
    12541 ggcaaggcct ggcagcccta tcatcaagca ttactggagc agtcacacaa gtaggtataa
    12601 cgggtatcgg tgccaaaaaa acgcattcag ggattagcga ccaaaaagga gccttaagaa
    12661 agaaccttgc cactgctcaa tctcttgaaa aagagcttgc aggttctaaa ttagggttaa
    12721 ataaacaaat agatacaaat atcacctcac cacaaactaa ctctagcaca aaatttttag
    12781 gtaaaaataa actggcgcca gataatatat ccctgtcaac tgaacataaa acttctctta
    12841 gttctcccga tatttctttg caggataaaa ttgacaccca gagaagaact tacgagctca
    12901 ataccctttc tgcgcagcaa aaacaaaaca ttggccgtgc aacaatggaa acatcagccg
    12961 ttgctggtaa tatatccaca tcaggagggc gttatgcatc tgctcttgaa gaagaagaac
    13021 aactaatcag tcaggccagc agtaaacaag cagaggaagc atcccaagta tctaaagaag
    13081 catcccaagc gacaaatcaa ttaatacaaa aattattgaa tataattgac agcatcaacc
    13141 aatcaaagaa ttcgacagcc agtcagattg ctggtaacat tcgagcttaa tatatccaag
    13201 agccataata atatatggct cttcctgtaa ggaaataacc atgaatataa caactctgac
    13261 taatagtatt tccacctcat cattcagtcc aaacaatacc aacggttcat caaccgaaac
    13321 agttaattct gatataaaaa caacgaccag ttctcatcct gtaagttccc ttactatgct
    13381 caacgacacc cttcataata tcagaacaac aaatcaggca ttaaagaaag agctttcaca
    13441 aaaaacgttg actaaaacat cgctagaaga aatagcatta cattcatctc agattagcat
    13501 ggatgtaaat aaatccgctc aactattaga tattctttcc aggcacgaat atccaattaa
    13561 taaagacgca agagaattat tacattcagc tccgaaagaa gccgagcttg atggagatca
    13621 aatgatatct catagagaac tgtgggctaa aattgcaaac tccatcaatg atattaatga
    13681 acagtatctg aaagtatatg aacatgccgt tagttcatat actcaaatgt atcaagattt
    13741 tagcgctgtt ctttccagtc ttgccggctg gatctctccc ggaggtaacg acggaaactc
    13801 cgtgaaatta caagtcaact cgcttaaaaa ggcattggaa gaactcaagg aaaaatataa
    13861 agataaaccg ctatatccag caaataatac tgttagtcag gaacaagcaa ataaatggct
    13921 tacagaatta ggtggaacaa tcggcaaggt atctcaaaaa aacgggggat atgttgtcag
    13981 tataaacatg accccaatag acaatatgtt aaaaagctta gataatctag gtggaaatgg
    14041 cgaggttgtg ctagataatg caaaatatca ggcatggaat gccggattct ctgccgaaga
    14101 tgaaacaatg aaaaataatc ttcaaacttt agttcaaaaa tacagtaatg ccaatagtat
    14161 ttttgataat ttagtaaagg ttttgagtag tacaataagc tcatgtacag atacagataa
    14221 actttttctc catttctgag gtgcgccatg cataatgtaa ataatactca agcgccaaca
    14281 ttcttatata aggcaacttc accatcatca acagaataca gcgagttaaa aagcaaaata
    14341 tccgatatcc atagttcgca aacttctcta aaaacaccag catcagtgtc tgaaaaagaa
    14401 aactttgcaa cgtcttttaa tcagaaatgt cttgattttt tattttcttc ctcagggaaa
    14461 gaagatgtgt taagaagcat ttattccaac tcaatgaatg cgtatgccaa aagcgagatt
    14521 ctcgaatttt caaatgtttt gtactcctta gtacatcaaa atggtcttaa ttttgaaaac
    14581 gaaaagggac ttcaaaaaat tgtcgcacag tattcggaac taattataaa agataaatta
    14641 tcccaagatt ctgcctttgg accatggtcg gcaaagaata agaaactcca tcaattacga
    14701 caaaacattg agcacagact tgcactatta gcacaacaac acacatctgg tgaagcttta
    14761 tcattgggac aaaaactcct caatactgaa gtatcatcat ttatcaagaa taatattctt
    14821 gctgaattaa agttaagtaa tgaaactgtt tcatctctca aactagatga tttagttgac
    14881 gcacaggcaa aacttgcctt tgatagtttg cgcaatcaac gtaaaaatac tattgatagt
    14941 aaaggatttg gtataggtaa actgtcaaga gacttaaata cagtagccgt gtttcctgag
    15001 ctgttgagaa aagtccttaa tgatatttta gaagatataa aagattcgca tcctatccaa
    15061 gatggcctcc ctacacctcc cgaagatatg ccagatggcg gaccaacccc cggagccaat
    15121 gagaaaacat cccaacctgt aattcactat catataaata atgataatag aacttacgat
    15181 aatagagttt ttgacaacag agtatatgac aatagctatc acgagaaccc agaaaatgat
    15241 gcacagtctc ctacttctca gacaaacgat ctattatccc gtaacggaaa ctcattacta
    15301 aatccacaaa gagcactagt tcaaaaagta acttccgttc taccacactc tatatcagat
    15361 actgtccaga catttgcaaa taattcagct ttagaaaagg ctttcaacca tactccagat
    15421 aattcggatg gaataggttc agacctgtta actacgagta gtcaagaaag atctgcaaat
    15481 aactctcttt ctcggggaca caggcctctg aacatacaga actcttcaac caccccccct
    15541 ctccacccgg aaggagtgac aagcagtaat gataactcat cagatacaac taaaagtagc
    15601 gcttctcttt ctcatagagt agcttcgcaa atcaataaat tcaactcaaa cactgattca
    15661 aaagtacttc agactgattt tttatcaaga aatggagaca catatttaac acgggaaacg
    15721 atatttgaag cttcaaaaaa agtaacaaac tccctaagta atcttatatc tctcattgga
    15781 actaaatcag gaacacaaga acgagagtta caggaaaaat caaaggacat tacaaaatcc
    15841 acaacagaac atagaataaa caacaaatta aaagttacag atgcaaatat aagaaactac
    15901 gtaacagaaa ccaacgcaga tacaattgat aaaaatcatg cgatctatga aaaggcaaaa
    15961 gaagtatcta gcgccctcag caaggtattg tcaaaaattg acgatacctc tgcagaatta
    16021 cttacagatg atatatctga tttaaaaaat aacaatgata ttacagctga aaacaataat
    16081 atatataaag cagcaaaaga tgtaaccact tccctatcaa aagtattaaa gaatatcaat
    16141 aaggattaaa atatgataaa agaaaaaata ttatcaatag tggccttttg ctatggtata
    16201 gcgtacagca aactatctga agaaactaaa tttattgaag atctttctgc cgattctctt
    16261 tctctgattg aaatgctgga tatgattagt tttgagttca atctacgtat agatgaatct
    16321 acattagaac acattattac tattggagat cttatttctg tagtcaaaaa tagtacaaaa
    16381 tcaatatagt aattgataag cattttttca tctatggagc tctcacatca gagctccaca
    16441 agaatattat tcttttatcc aataaagata aattgcatca atccagctat taaaatagta
    16501 atttttaaga ctaccgttga ctatcatcaa cagttacata caatcttgct cacactgcat
    16561 ttaacttttg tcaatataaa tcaatatgaa taaacagggt gtgatatggt ggatttgtgc
    16621 aacgacttgt taagtataaa ggaaggccaa aagaaagagt ttacactcca ttctggtaat
    16681 aaagtttcct ttatcaaagc caagattcct cataaaagga tccaagattt aaccttcgtc
    16741 aaccaaaaaa cgaatgtacg cgatcaagaa tccctaacag aagaatcatt agccgatatc
    16801 ataaaaacta taaagctaca acaattcttc cctgtaatag gaagggagat tgatggtaga
    16861 attgaaattc tggatggcac tcgtagaaga gcatctgcaa tatatgcagg agcagatctt
    16921 gaagttctat attcaaaaga atatatatct actcttgatg ccagaaaact agcaaacgat
    16981 atacaaacag caaaagagca tagcatccga gaacttggta ttggtcttaa ttttctgaaa
    17041 gtatcaggga tgtcctataa agacatagcc aaaaaagaga atctgtctcg cgcgaaagtc
    17101 actcgtgcct ttcaggcagc aagcgttcca caggaaataa tatctctatt tccaatcgcg
    17161 tcagaactta actttaatga ctacaagata ttattcaatt attataaagg acttgaaaag
    17221 gctaatgaat ctcttagttc tacactacca atattaaagg aagaaataaa agaccttgat
    17281 acaaatttgc ccccggacat atataaaaaa gaaattttaa acatcataaa gaaaagcaaa
    17341 aacagaaagc aaaacccttc gctaaaagtt gactcgttat ttatttctaa agacaaacgg
    17401 acttacataa aaagaaaaga gaataaaaca aacagaactc taatatttac attatctaaa
    17461 ataaacaaaa cagttcagag agaaatagat gaagctattc gggatataat ttctcgccat
    17521 ctatcgtctt cataaacatc gaacactgat gtaaactgcc caattcagat aataaaaatc
    17581 ttcgttgaac ttgactttca gccggtgggg taattccgtc ggctatctgt tattcaatcc
    17641 ccacgcactg tgtttcctta aaattacggt gaaaactcat accctgctga ccacgagaaa
    17701 attatctggt gaaattagta atattcatat cattgaaaat gaattgtgca actaagggat
    17761 gcggcatgat ggtcaaaatt ttatgtgaat atgagtgact ggccgaaaat attcagcgta
    17821 tttttaatcg tttcaataaa aacagcgagg attagcagtc aacgctttta ctactcaatt
    17881 ttttttacat ggtgataaac aaaaaatcca ccattaacag acttcgttca cagtatcaaa
    17941 aaagctttaa gaatatatta gcagatttct caaatataat tactgtttta ttctcctaat
    18001 cttcccataa aatgaccgac ttacattatt ttataattat aaacaagttt gattttaaaa
    18061 tcatattaat tattaacaag agatttattt ttctaacccc aaaagttatg cttggtcaaa
    18121 gagtaactta taatatccat acccctcata gggcttacag tttaaattgc gggtataaaa
    18181 tacatctatt ttcaaataaa ataaagcata ctccaattac aacgagtgac taaaataaaa
    18241 atatcatcca agtcaatcac tgtttgctct gcaaaatatg tcagccttct caaacttacc
    18301 agcacttaaa tatttaagca cacaatcatt ccttgaggga gttcacttat ttcaagagaa
    18361 acatttcgtc ttcccacaca actaatccgc ccaatatagc taaatttgta ctcagtaata
    18421 tcatttgtta tgcccccccc aaaatattca agctccttca cagataaaaa acgacaattt
    18481 acaaagcctc attagttata actatggaaa tacctatttt agtatatcct ataaagttag
    18541 aattacttct tcttgcacct aattccatag ttgaaaaaca agaataattt tctcctacag
    18601 ctggatccat atagcttcca tcaggtctac acaacaccca gtggaggccg acaggaactc
    18661 caataaagga tacagcaaca gcttccaaaa ctctttggtt agaagataac acatctcgtt
    18721 gatgtactat attacatccc atgccaatta ataagtccct tgcatcggga taaataaaac
    18781 taattacttg cgaaaaaatg ttactttctt caacgacata agcattcaat ccaagaagcc
    18841 tcgtagccat gactatctta tcaggcatag aataaccggc atccgcgata ttatctttgt
    18901 gtattctcgg attataatta ccactagtta ttaagtataa atcccgctca caacgattat
    18961 ccaaatcaag agaactcttc cttgtcattt cggacataga ccctttgtat tgtggtattt
    19021 tatcaacccc taattccttt gcagcacaga gtaaactggc agctccacaa gaattgtcat
    19081 acatctgctt cctcgccaaa aaacaacctt gttcgtttac tctattctca tttaccctca
    19141 catcagtctg atgagatatc atatattctc ttggtatagc cccctgcaat aaaaccccat
    19201 ataacataac acctgtatga atacatcccc gtttgcaagg cttccgttgt tccgacatcg
    19261 acgagtaatt gcagcacagt attgcatcca gcaatgcgaa ctactatgag aattagctga
    19321 ccgggtcctt attccccgaa cgaactttaa atccaaaacc tcatactctt ttccgtcgtc
    19381 atcacgtccg tcaccataac aacctttcag catccaccct tttattaaga gttaatgtcc
    19441 atcagcatca gcaattaata ccgcctgata ctgacatatt tttgtcgtat ttacgtccat
    19501 aatcggttat tgtgatacag gtcacaccag cttatttgct attgttacaa taaccacctt
    19561 ggttgagagc tgttttttca actttgttat caactccccc aactccttgg tcagtaaggc
    19621 tgccgaccga gcacagtgaa tagatagtat ccgcaggtac caccatgctt acttaatact
    19681 cagaaggcga agtgctctcc aattaactct gccgcactat gcttataagc tgcttttcaa
    19741 tgccgtctat caacatgtcc agttcattca atctgttgta ctggtaatcc aacgcagtaa
    19801 tgatatatgc aggtattctt tcatccactc cagtaacgtt tcatgcaaag cattaattta
    19861 cgcgctacgg aacttcacca gttgactgag caaacgatgt gggaccagta ctgcctgcag
    19921 ttcttctatt gttacagcga tttctttacc aaacagctga accaccatca agataaagat
    19981 agcctgaaca ttcatcgcac cattttgtta ccaatggcaa aagatttaac gaaccgtgcc
    20041 tgcatcaggc ggacttttta tccctgcttt gttagctccc taaccttgta cagagagcct
    20101 ccataagctc ccatatcaag cagacatggt tcgcagttac tgacatacac cagaaaactc
    20161 tgacagcaga ctcacttatc ctccacttca ctcatatcct tattggtgag atgaacctga
    20221 atgacatgtt ttgcgacata aacgccaacc agtgtatatt tcacctgtag agattctaca
    20281 atctgtagcg ttatgcatct aatgttaggc tctatgatgt cagaaatctg cgagactcta
    20341 caccttctgc atttaccttt atttatcacc atgctcctca gttaacggag aacgtgttca
    20401 ttgcattttg tcacttacat ttcgcctttg gtatcggggg gatataggtt ccaacgttcc
    20461 gcatgagaac ctgaaccggc ttcacgccac cttgatacca gatgccgcac aggtagtcaa
    20521 caagtgtcac gaacggtgca atagtgatcc acacccaacg cctgaaatca gatccagggg
    20581 gtaatctgct ctcctgattc aggagagctt atggtcactt ttgagacagt tatggaaatt
    20641 aaaatcctgc acaagcaggg aatgagtagc cggacgattg ccagagaact ggggctctcc
    20701 cgcaataccg ttaaacgtta tttgcaggca aaatctgagc cgccaaaata tacgccgcga
    20761 cctgctgttg cttcactcct ggatgaatac cgggattata ttcgtcaacg catcgccgat
    20821 gctcatcctt acaaaatccc ggcaacggta atcgctcgcg agatcagaga ccagggatat
    20881 cgtggcggaa tgaccattct cagggcattc attcgttctc tctcggttcc tcaggagcag
    20941 gagcctgccg ttcggttcga aactgaaccc ggacgacaga tgcaggttga ctggggcact
    21001 atgcgtaatg gtcgctcacc gcttcacgtg ttcgttgctg ttcccggata cagccgaatg
    21061 ttgtacatcg aattcactga caatatgcgt tatgacacgc tggagacctg ccatcgtaat
    21121 gcg
//
LOCUS       DAJTPG010000068        20769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTPG010000068 DAJTPG010000000
VERSION     DAJTPG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..579
                     /locus_tag="OO230_002608"
     CDS             <1..579
                     /locus_tag="OO230_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5692316.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO230_002609"
     CDS             597..1235
                     /locus_tag="OO230_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5692317.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO230_002610"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO230_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5692318.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO230_002611"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO230_002611"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5692319.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO230_002612"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO230_002612"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5692320.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO230_002613"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO230_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5692321.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO230_002614"
     CDS             complement(5552..6613)
                     /locus_tag="OO230_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5692322.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO230_002615"
     CDS             complement(6610..8007)
                     /locus_tag="OO230_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5692323.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO230_002616"
     CDS             8163..9161
                     /locus_tag="OO230_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5692324.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO230_002617"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO230_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO230_002618"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO230_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO230_002619"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO230_002619"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5692325.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO230_002620"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO230_002620"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5692326.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO230_002621"
     CDS             complement(14232..14855)
                     /locus_tag="OO230_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5692327.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO230_002622"
     CDS             complement(14852..15274)
                     /locus_tag="OO230_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5692328.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO230_002623"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO230_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO230_002624"
     CDS             complement(16089..17141)
                     /locus_tag="OO230_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5692329.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO230_002625"
     CDS             complement(17172..18014)
                     /locus_tag="OO230_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5692330.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO230_002626"
     CDS             complement(18001..18882)
                     /locus_tag="OO230_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5692331.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO230_002627"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO230_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20769)
                     /gene="ycjM"
                     /locus_tag="OO230_002628"
     CDS             complement(20200..>20769)
                     /gene="ycjM"
                     /locus_tag="OO230_002628"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5692332.1"
                     /translation="KNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAILLS
                     FPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAVYHE
                     LSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVNIT
                     DLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5068 a         5665 c         5052 g         4984 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attattttt
//
LOCUS       DAJTPG010000069        20708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTPG010000069 DAJTPG010000000
VERSION     DAJTPG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO230_002629"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OO230_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5692333.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO230_002630"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OO230_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5692334.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO230_002631"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OO230_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5692335.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO230_002632"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OO230_002632"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5692336.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OO230_002633"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OO230_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5692337.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OO230_002634"
     CDS             complement(8217..9110)
                     /locus_tag="OO230_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5692338.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO230_002635"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OO230_002635"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5692339.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO230_002636"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OO230_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5692340.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OO230_002637"
     CDS             complement(10241..11473)
                     /locus_tag="OO230_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5692341.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO230_002638"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OO230_002638"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5692342.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO230_002639"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OO230_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5692343.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OO230_002640"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OO230_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO230_002641"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OO230_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5692344.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO230_002642"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OO230_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5692345.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO230_002643"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OO230_002643"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5692346.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO230_002644"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="OO230_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5692347.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5726 c         5030 g         4971 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaat
//
LOCUS       DAJTPG010000070        20692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTPG010000070 DAJTPG010000000
VERSION     DAJTPG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            82..351
                     /locus_tag="OO230_002645"
     CDS             82..351
                     /locus_tag="OO230_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5692348.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO230_002646"
     CDS             345..710
                     /locus_tag="OO230_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5692349.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO230_002647"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO230_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5692350.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO230_002648"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO230_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5692351.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO230_002649"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO230_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5692352.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO230_002650"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO230_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5692353.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO230_002651"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO230_002651"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5692354.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TTTKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO230_002652"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO230_002652"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5692355.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO230_002653"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO230_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO230_002654"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO230_002654"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5692356.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO230_002655"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO230_002655"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5692357.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14850..16448
                     /gene="rtcR"
                     /locus_tag="OO230_002656"
     CDS             14850..16448
                     /gene="rtcR"
                     /locus_tag="OO230_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5692358.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16430..17188)
                     /locus_tag="OO230_002657"
     CDS             complement(16430..17188)
                     /locus_tag="OO230_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5692359.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO230_002658"
     CDS             complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO230_002658"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5692360.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO230_002659"
     CDS             complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO230_002659"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5692361.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18596..20101
                     /gene="glpD"
                     /locus_tag="OO230_002660"
     CDS             18596..20101
                     /gene="glpD"
                     /locus_tag="OO230_002660"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5692362.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20370..20600)
                     /locus_tag="OO230_002661"
                     /pseudo
     CDS             complement(20370..20600)
                     /locus_tag="OO230_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4894 a         5692 c         5213 g         4893 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcatggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caagtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggtggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc acttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctag
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaatttat tgtttctaaa
    14761 aagattatat ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg
    14821 aaatatcgcc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    14881 gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac
    14941 tctgtatata ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc
    15001 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag
    15061 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    15121 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    15181 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt
    15241 atctacccgc ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc
    15301 caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    15361 ttgccgagga acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc
    15421 cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga
    15481 ttctgcttaa cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt
    15541 taaaacaggc acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    15601 gcggcgatac cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    15661 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    15721 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct
    15781 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    15841 tgcgcgattt gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga
    15901 tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga
    15961 acctggatta tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca
    16021 ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta
    16081 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    16141 tcactctgga cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc
    16201 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    16261 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    16321 gacgacagct ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    16381 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    16441 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg
    16501 tgtagacggc atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt
    16561 ttgaatggtc aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac
    16621 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca
    16681 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    16741 cgccatcgcg gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca
    16801 tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca
    16861 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    16921 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    16981 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca
    17041 ggatcaggtt ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg
    17101 agaagtgctc caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    17161 taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc
    17221 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct
    17281 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    17341 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    17401 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    17461 aatttttgct gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    17521 ccgctaccga ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag
    17581 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    17641 ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt
    17701 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    17761 cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    17821 cctgccagcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    17881 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    17941 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    18001 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    18061 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc
    18121 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    18181 gcgccgcccc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    18241 cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    18301 gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    18361 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    18421 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    18481 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    18541 taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga
    18601 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    18661 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    18721 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    18781 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc
    18841 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    18901 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    18961 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    19021 tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa
    19081 aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg
    19141 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    19201 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc
    19261 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca
    19321 aaagcaggcc tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat
    19381 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt
    19441 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    19501 gcagctaagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    19561 tgagtccgac tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa
    19621 cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    19681 agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa
    19741 agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct
    19801 gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg
    19861 cagcaacagc gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt
    19921 cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg
    19981 caccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    20041 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta
    20101 aattaacgta aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa
    20161 aactgcctga tgcgctacgc ttatcaggcc tacgtggttt atgcaatata ttgaatttgc
    20221 atggtcttgt aggccagata agacgttcac gtcgcatccg gcatgaacaa agcgcacttt
    20281 gtcaaaaatc taacctactt tttaattcag gaaattaccg caaagcccac gccatcatac
    20341 ccaacgtaac aaaactcagg cacgttcccc actcgccccg agaaaaatag cattaatgcg
    20401 cccagcgtca gcataaaaat attgagcaga aggtaaattg ttggcgtaaa gagtaaattg
    20461 caaacctcag gaatagcggc aagcccagcc cccagcatgg caataaccgc tgcaaaaaat
    20521 tgcgtcagta ttgggatgcc gaaaaaagtc attaccagcg aggtcaaaat ccattttcta
    20581 ttttgcatta ttctttccat tctttctgaa tggcgaaatt atactccccg agtccccttg
    20641 ccccttctgg acacttttcc gaaatgatgg cgaaaaaaac gggacccttt gg
//
LOCUS       DAJTPG010000071        20612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTPG010000071 DAJTPG010000000
VERSION     DAJTPG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            96..395
                     /locus_tag="OO230_002662"
     CDS             96..395
                     /locus_tag="OO230_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5692363.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="OO230_002663"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="OO230_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5692364.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO230_002664"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO230_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5692365.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="OO230_002665"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="OO230_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5692366.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="OO230_002666"
     CDS             complement(2534..2878)
                     /locus_tag="OO230_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5692367.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="OO230_002667"
     CDS             3030..3260
                     /locus_tag="OO230_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692368.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="OO230_002668"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="OO230_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5692369.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="OO230_002669"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="OO230_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5692370.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="OO230_002670"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="OO230_002670"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5692371.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="OO230_002671"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="OO230_002671"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5692372.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="OO230_002672"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="OO230_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5692373.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="OO230_002673"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="OO230_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5692374.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="OO230_002674"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="OO230_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5692375.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="OO230_002675"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="OO230_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO230_002676"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO230_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5692376.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="OO230_002677"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="OO230_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5692377.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="OO230_002678"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="OO230_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5692378.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="OO230_002679"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="OO230_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="OO230_002680"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="OO230_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5692379.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO230_002681"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO230_002681"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5692380.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO230_002682"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO230_002682"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5692381.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="OO230_002683"
     CDS             complement(19399..19827)
                     /locus_tag="OO230_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5692382.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO230_002684"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO230_002684"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5692383.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4973 a         5273 c         5149 g         5217 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taaacggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat acaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag gcagcgatat acgctatgca aaaatgcaga
     1981 tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaagaaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaagctg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg at
//
LOCUS       DAJTPG010000072        20454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTPG010000072 DAJTPG010000000
VERSION     DAJTPG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            186..527
                     /gene="raiA"
                     /locus_tag="OO230_002685"
     CDS             186..527
                     /gene="raiA"
                     /locus_tag="OO230_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5692384.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    630..755
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            631..678
                     /gene="pheL"
                     /locus_tag="OO230_002686"
     CDS             631..678
                     /gene="pheL"
                     /locus_tag="OO230_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5692385.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            777..1937
                     /gene="pheA"
                     /locus_tag="OO230_002687"
     CDS             777..1937
                     /gene="pheA"
                     /locus_tag="OO230_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5692386.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1980..3101)
                     /gene="tyrA"
                     /locus_tag="OO230_002688"
     CDS             complement(1980..3101)
                     /gene="tyrA"
                     /locus_tag="OO230_002688"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5692387.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3112..4182)
                     /gene="aroF"
                     /locus_tag="OO230_002689"
     CDS             complement(3112..4182)
                     /gene="aroF"
                     /locus_tag="OO230_002689"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5692388.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4392..4757
                     /locus_tag="OO230_002690"
     CDS             4392..4757
                     /locus_tag="OO230_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5692389.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4907..5425
                     /locus_tag="OO230_002691"
     CDS             4907..5425
                     /locus_tag="OO230_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5692390.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5415..6640
                     /gene="dgcN"
                     /locus_tag="OO230_002692"
                     /pseudo
     CDS             5415..6640
                     /gene="dgcN"
                     /locus_tag="OO230_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6656..7138
                     /locus_tag="OO230_002693"
     CDS             6656..7138
                     /locus_tag="OO230_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5692391.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7215..7562)
                     /gene="rplS"
                     /locus_tag="OO230_002694"
     CDS             complement(7215..7562)
                     /gene="rplS"
                     /locus_tag="OO230_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5692392.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7604..8371)
                     /gene="trmD"
                     /locus_tag="OO230_002695"
     CDS             complement(7604..8371)
                     /gene="trmD"
                     /locus_tag="OO230_002695"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5692393.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8402..8950)
                     /gene="rimM"
                     /locus_tag="OO230_002696"
     CDS             complement(8402..8950)
                     /gene="rimM"
                     /locus_tag="OO230_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5692394.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8969..9217)
                     /gene="rpsP"
                     /locus_tag="OO230_002697"
     CDS             complement(8969..9217)
                     /gene="rpsP"
                     /locus_tag="OO230_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5692395.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9354..10715)
                     /gene="ffh"
                     /locus_tag="OO230_002698"
     CDS             complement(9354..10715)
                     /gene="ffh"
                     /locus_tag="OO230_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5692396.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10882..11673
                     /locus_tag="OO230_002699"
     CDS             10882..11673
                     /locus_tag="OO230_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5692397.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11694..12980
                     /locus_tag="OO230_002700"
     CDS             11694..12980
                     /locus_tag="OO230_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5692398.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13035..13628)
                     /gene="grpE"
                     /locus_tag="OO230_002701"
     CDS             complement(13035..13628)
                     /gene="grpE"
                     /locus_tag="OO230_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5692399.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13751..14629
                     /gene="nadK"
                     /locus_tag="OO230_002702"
     CDS             13751..14629
                     /gene="nadK"
                     /locus_tag="OO230_002702"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5692400.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14715..16376
                     /gene="recN"
                     /locus_tag="OO230_002703"
     CDS             14715..16376
                     /gene="recN"
                     /locus_tag="OO230_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5692401.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16525..16866
                     /gene="bamE"
                     /locus_tag="OO230_002704"
     CDS             16525..16866
                     /gene="bamE"
                     /locus_tag="OO230_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5692402.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16928..17218)
                     /locus_tag="OO230_002705"
     CDS             complement(16928..17218)
                     /locus_tag="OO230_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5692403.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17208..17645)
                     /gene="ratA"
                     /locus_tag="OO230_002706"
     CDS             complement(17208..17645)
                     /gene="ratA"
                     /locus_tag="OO230_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5692404.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17816..18298
                     /gene="smpB"
                     /locus_tag="OO230_002707"
     CDS             17816..18298
                     /gene="smpB"
                     /locus_tag="OO230_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5692405.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18513..18875
                     /gene="ssrA"
                     /locus_tag="OO230_002708"
     tmRNA           18513..18875
                     /gene="ssrA"
                     /locus_tag="OO230_002708"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19080..20321
                     /locus_tag="OO230_002709"
     CDS             19080..20321
                     /locus_tag="OO230_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5692406.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5285 a         5346 c         4801 g         5022 t
ORIGIN      
        1 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
       61 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gatttccatc
      121 acacattttg acatcaggaa cggtatgctg aattcaccaa gacgggaaga caagaggtaa
      181 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
      241 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
      301 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
      361 gcgttctggt tgccagtggt aaacatgaag atatgtacac cgcaattaac gaattgatca
      421 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
      481 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
      541 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
      601 actgtactaa agtcacttaa ggaaacaaac atgaaacaca taccgttttt cttcgcattc
      661 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
      721 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
      781 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
      841 tagcgttact ggcagaacgg cgcgaactgg ccgtcgaggt gggaaaagcc aaactgctct
      901 cgcatcgccc ggtacgtgat attgatcgtg aacgcgattt gctggaaaga ttaattacgc
      961 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     1021 aagattccgt attaactcag caggctttgc tccaacaaca tctcaataaa attaatccgc
     1081 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     1141 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     1201 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     1261 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     1321 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcactact gatttatcca
     1381 ccatcaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     1441 gttatccgca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     1501 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     1561 gtttgcaggt actggagcgt attgaagcaa atcagcgaca aaacttcacc cgatttgtgg
     1621 tgttggcgcg taaagccatt aacgtgtctg atcaggttcc ggcgaaaacc acgttgttaa
     1681 tggcgaccgg gcaacaagcc ggtgcgttgg ttgaagcgtt gctggtactg cgcaaccaca
     1741 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     1801 tctatctgga tattcaggct aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     1861 taggggaaat cacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     1921 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     1981 tactggcgat tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     2041 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     2101 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     2161 agattacgct ctgacgacat aatgatgtcg gcataaagct gcggatcctg agcaaacagt
     2221 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     2281 agctgaacat tttcttctgc caggtgcagc ccgtaagcaa aagtagcaaa gtggcgcaga
     2341 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg caaccgagcg
     2401 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     2461 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     2521 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     2581 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     2641 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     2701 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     2761 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     2821 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     2881 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     2941 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcaccttg
     3001 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     3061 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     3121 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     3181 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     3241 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     3301 attaccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     3361 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     3421 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     3481 caggatcaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     3541 gttaatgcca acaaaacagt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     3601 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     3661 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     3721 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     3781 cagctcaagc aacaatttac gcgcgatttg cagcccggct tctacatcaa aagagccatc
     3841 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     3901 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagagctt taaatcgacg
     3961 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     4021 acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag caatctgggc
     4081 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     4141 ttcgtcggta atatgtacgt tattcagcgc gtctttatgc atgatggcga tcctgtttat
     4201 gctcgtttgc gatagttgct cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     4261 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gctttcaacc
     4321 cttgcttctg tatagataaa tttacatccc ctttaaaaac aatccgctat gctttgaaaa
     4381 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
     4441 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgtcg
     4501 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
     4561 aggcggatcg cggtctatac cttaaaggat atgccgaagg gcaaaagaaa acttgccaga
     4621 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
     4681 ttgaaaatgc cagccaactg catgaagtat ggcaaaaaag ggctgatgaa aatgccagca
     4741 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
     4801 tgttttattt ctcacattga tgacggtcgc atgaaaaaat gatattttaa tgagcatcct
     4861 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
     4921 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaagagcc
     4981 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
     5041 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
     5101 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
     5161 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
     5221 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
     5281 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
     5341 cgtaaacctg gatgcctcat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
     5401 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
     5461 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
     5521 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
     5581 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
     5641 caactgaaac actggcagcg ctgggccagc aagggcattt tcaactgcag aagtacgtga
     5701 taagcagcaa aatattctgg catcctggca ttacccccgt aaggatccag gcgatacttt
     5761 cagcaatttc ataagccact ggctcttccc tgcccccatc attcagccga ttcgtcacaa
     5821 tggtgaaacc attggcgaag tacgcttaac cgctcgcgac agttcaatca gccattttat
     5881 ctggttttcg ctcgccgtac tgaccggttg tattctgctg gcatcaggca tcgcaattac
     5941 cctcacccgc catttgcaca atggcctggt ggaagcactg aaaaatatca ccgatgtcgt
     6001 acatgatgtg cgttccaacc gcaatttttc ccgacgagtt tcggaagaac gtatcgctga
     6061 gtttcaccgc ttcgctctcg acttcaacag tctgctggat gaaatggaag agtggcagct
     6121 tcgtttacag gccaaaaatg cgcagcttct gcgtaccgcg ctacatgacc cattaaccgg
     6181 gctggctaac cgcgcagcgt ttcgtagcgg catcaatacg ttgatgaaca attccgatgc
     6241 ccgaaaaacg tcggcgttac tatttcttga tggcgataat ttcaaatata tcaatgatac
     6301 ctggggtcat gcgacgggcg atagggtctt gattgaaatc gcaaaacggt tagctgaatt
     6361 tggcgggctg cgacataaag cataccgcct gggcggcgat gaattcgcta tggtgctcta
     6421 tgatgtacag tcggaatctg aagtgcagca gatatgctca gcactgacac aaatctttaa
     6481 tctcccgttt gatcttcata atggccatca gaccaccatg acattaagca ttggttacgc
     6541 gatgaccatt gagcacgcct ctgcggaaaa attacaagag cttgccgatc acaatatgta
     6601 tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa caaggatata tcgatatgat
     6661 aaagcacctg gtagcacccc tgattttcac ctcactaata ctgactggct gccagtcccc
     6721 tcagggaaag tttactcctg agcaagtcgc cgctatgcaa tcttatggat ttactgaatc
     6781 cgccggcgac tggtcgctgg gcttatcaga tgccattctg ttcgcaaaaa atgactacaa
     6841 attgctcccg gaaagccagc aacagatcca aaccatggca gccaaattgg cctcgacagg
     6901 gctaacacat gcccgtatgg atggacacac cgataactat ggtgaagaca gttacaacga
     6961 aggcttatca ttgaaacggg cgaatgtcgt ggccgatgca tgggctattg gtggacaaat
     7021 tccacgcagc aatctcacca cacagggttt aggaaaaaaa tatcccatag ccagtaacaa
     7081 gaccgcccag ggccgcgccg agaaccgccg cgtcgcagtg gtgattgcaa ccccttaaaa
     7141 cacgagcaaa cagacaaaaa aagggccagc caattggcca gcccttctta acaggatgtc
     7201 gcttaagcga aatcttagtt aagacgctct ttgatacgag cagccttacc agtacgctca
     7261 cgcaggtagt acagtttagc tttacgaaca gcaccacgac gtttgacaga aatgctgtca
     7321 actaccggag agtgagtctg gaagacacgc tcaacgcctt cgccgttgga aattttacga
     7381 acagtgaatg cagagtgcag accgcggtta cgaatagcga taaccacgcc ctcgaatgcc
     7441 tgcagacgtt ttttggaacc ttcaacaacc catactttca cttccacggt atcacccgga
     7501 cggaaggaag gtacgtcctg cttcatctgc tcttgttcaa gttgcttaat aatgttgctc
     7561 ataatttaat ctcttatcct gggtaaactg atctctcggg ggcttacgcc atcccatcat
     7621 gtttatgttg ctgttgtgcg tgttccgttt tgaactccgc cagcaacctt gcttgctctt
     7681 cagtcagagc caggttttcc agaagttcag gtcttctaag ccaggtacgg cccagcgact
     7741 gtttcaaacg ccagcgacgt atctcggcat ggttgcccga cagtaacact ggcggaactt
     7801 ccatcccttc taacacctca ggccgcgtat agtgcgggca atccagcaat ccttcagcaa
     7861 aggaatcttc cgttgccgag gcttcatgtc ccagtactcc cggaataaac cgggaaacgg
     7921 agtcaatcag cgtcattgct ggtaactcac caccactgag aacgtaatcg ccgattgacc
     7981 attcttcgtc aatttcggtt tggatcacgc gctcatctat accttcgtag cgaccgcaca
     8041 ccagaatcaa tttttgattc gttgccagtt cgctgacgcc cgcttgatca agcttgcgtc
     8101 cctgtggtga cagataaatc acctttgcgc cttcacccgc cgcggctttt gctgcatgaa
     8161 tggcgtcccg caagggttgc accatcatta acatccccgg tccgccgccg taaggacgat
     8221 cgtccacggt acggtgccgg tcatgcgtga agtcgcgagg actccaactc tggatgctca
     8281 gcaggccatt tttaactgcc cggccagtta ccccgtaatc ggtaattgcg cggaacattt
     8341 caggaaacag gctaattatg ccaatccaca tagcgccgcc ttttaccgtt tatccggtgg
     8401 tttaaaaacc aggatcccaa tctacttcga ttgaacgagt agtgagatcg actttcttga
     8461 taacctgccc atcgaggaac ggtacgagac gttccttgat accaaacgca tctttcaggt
     8521 ttgccttaat gacgagaacg tcattagatc cggtttccat catatcaacg actttaccga
     8581 gatcgtagcc ttcagtggtt actacctggc agcccatcag gtctttccag tagtagtccc
     8641 cctcttcaag ctgaggcagc tgcgatgaat ccacgacaat ttcacaattc gtcagcaggt
     8701 tcgccgcatc acgatcgtca acgcctttca gcttgatgat catgtcctga ttgtggtgct
     8761 tccagctttc cagctggact tgctgccact gacccgcctt ctggataaac cagggctgat
     8821 agtcaaaaat gctttcggcg tcttcggtgg aagaaaacac tctgagccac ccacgaatac
     8881 cgtaagacga acccattttt cccaaaacga tgggatcaac aggtgcttgc gcggtgagtt
     8941 gtttgctcat catgaccacc gtgacagatt aagctgcttt gtttacttct ttgatcagcg
     9001 cagcaacgcg atcagaaata gttgcgccct ggccaaccca gtgagcgatg cgatccagat
     9061 ccaggcgagt gccttcttct ttttcgctag cgattgggtt gaagaaacca acgcgctcga
     9121 tgaagcgacc gttgcgtgca ttacggctgt cagcgacaac aacctggtag aacggacgct
     9181 ttttagcgcc gtgacgtgct aaacgaatag ttaccataac atcctcttgt gtgaataaaa
     9241 caaccggacc ccatcgagga acggagtccg gtgtcatatt aaaagcccga aaattttact
     9301 gatttctgga gaaaatgcaa tcagcagtta ataactctgt cgcataaggt catttagcga
     9361 ccaggaaatc ccggcggcat cattcccttc atgcttctca tcatcttcgc cattccgcct
     9421 ttcttcattt tcttcatcat gcgctgcatg tcgtcgaact gcttcagaag gcggttaacg
     9481 tcctgtacct gcatcccgca accggcggcg atacgacgct tacgcgaacc tttgatgatt
     9541 tctggcttag cgcgctcttt catcgtcatt gagttgatga tggcttccat acgcaccagc
     9601 actttatcgt ccatctgcga cttgacgtta tccgggatct gccccatgcc cggcagtttg
     9661 cccatcaagc tggccatgcc gcccatattt ttcatctggc gcagttgctc aagaaagtcg
     9721 ttgagatcga agccgtcgcc tttttttagc ttgctggcta atttctctgc ctgcgcgcgg
     9781 tcaactttgc tttcgatatc ttcgatcagc gacagtacgt cgcccatgcc gagaatacgc
     9841 gacgcgatgc ggtccggatg gaacggctcc agcgcctcag ttttctcgcc aacaccgagg
     9901 aacttgatcg gtttgccagt gatgtgacga atagagagcg ccgcaccgcc gcgggcatcg
     9961 ccgtccactt tggtcaacac tacgccggta agcggtaacg cttcattgaa tgcttttgcc
    10021 gtattggccg catcctgacc ggtcatggcg tcaaccacaa acagggtttc aaccgggtta
    10081 atcgacgcat ggacttgttt gatctcgtcc atcatcgctt cgtcaacgtg cagacgacca
    10141 gcggtatcca ccagcagcac gtcgtagaat ttcagtttgg cttctttcag cgccgcgtta
    10201 acaatatcaa ccggcttctg accaacatca gaagggaaga aatcaacgcc cacctgctct
    10261 gccagcgtct caagctgttt gattgccgcc gggcgataga cgtcagcaga aaccaccagc
    10321 actttcttct tgtgcttctc gcgcaggaaa ttaccgagtt taccaacgct ggttgtttta
    10381 ccggcacctt gcaggcctgc catcagcacg accgcaggcg gttgcgcagc cagattcagg
    10441 gtctggttct cttcgcccat cgccgcaacc agttcgttac ggacgatttt gacgaactcc
    10501 tgccccggcg tcaggctctt attaacttca tgaccaaccg ctttctcttt tacgcgattg
    10561 ataaactcac gcactaccgg cagagcgacg tccgcctcca gcagcgccat gcgcacttcg
    10621 cgcagcgtat cttttacgtt gtcttcagtg aggcgtccac ggccactgat attgcgcagc
    10681 gtgcgcgaca aacgatcggt taaattatca aacattgtct ctcgcctggg gtggaaacgg
    10741 ttggccgcaa tcgcgacaca tcatcagtat tttgccgcag tataacatga aggcgtcttt
    10801 gttgttatgc aacggttgga gcagcgttca cctgacgcta tactgcttct ctttcttatt
    10861 gctcaaactg tcgacattac tatgcccgtt tttgctctgc tcgcgcttgt cgcctactcc
    10921 gtcagtcttg cgctgattgt tcccggtctg ctgcaaaaaa acggcggctg gcggcgcatg
    10981 gctattattt ctgcggtcat tgcgctggtc tgccacgcaa tcgctctgga agcccgcatc
    11041 ctgcccgacg gtgatagcgg acaaaacctc agcctgctga acgttggttc attggtcagt
    11101 ttggtgatct gtacggtaat gaccattgtg gcttctcgca atcgtggctg gttgctgcta
    11161 cccattgtct atgcctttgc gcttatcaac ctggcgctgg cgaccttcat gcccaatgaa
    11221 tacatcaccc atctggaagc aacgcctggg atgctgatgc acattggctt atcgctcttt
    11281 tcctatgcca cgctaattat cgccgccctg tacgcgctgc aactggcgtg gattgattac
    11341 caactgaaga acaagaagct ggcatttaac caagaaatgc cgccattgat gagtatcgag
    11401 cgtaaaatgt tccacatcac gcagattggc gtggtgctgc taacgctcac gctttgcact
    11461 ggcctgttct acatgcacaa cctatttagc atggaaaata tcgacaaggc tgtgctctct
    11521 atcgtggcgt ggtttgtcta tattgtgctg ctgtggggac attaccatga aggatggcgt
    11581 ggacgccgcg tcgtctggtt taacgttgcg ggcgcggtaa ttctgacact ggcctacttc
    11641 ggcagccgaa ttgtccagca gttaatcagc taaacccaga aaaggagttt cccctggaac
    11701 acatttctac tactacgttg atcattattc tgatcatcat ggtggtcatt tcagcctatt
    11761 tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta tcgtcttcga catatggcga
    11821 aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct gcgtaagcca gaccgcctga
    11881 taagcctggt gttaatcggc aataacctgg tcaatattct tgcctccgcg ctcggcacta
    11941 ttgtcgggat gcgtttgtac ggcgatgcgg gcgtggcaat tgcgactggt gtgctgactt
    12001 ttgtcgtact ggtttttgct gaggtattgc cgaaaaccat tgccgcgctg tacccggaaa
    12061 aagtcgctta tccgagtagt tttctgctgg ctccgctgca aattttgatg atgccgctgg
    12121 tctggttgct gaatgctatc acccgtatgc tgatgcgcat gatgggtatc aaaaccgata
    12181 tcgtggttag cggctctttg agcaaagaag agttgcgcac tatcgtgcat gaatcgcgct
    12241 cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt gctcgatctg gaaaaaatga
    12301 ccgttgatga catcatggta ccgcgcagtg aaattatcgg tattgatatc aacgatgact
    12361 ggaaatctat tctgcgccaa ctctcccact cacctcacgg gcgcatcgtg ctttaccgtg
    12421 attcgctgga cgacgccatc agtatgctgc gagtacgtga agcctggcgg ttgatgtcgg
    12481 agaaaaaaga gttcaccaaa gaaaccatgc tgcgcgccgc ggacgagatc tattttgtgc
    12541 ctgaaggtac gccgctcagc acgcagttgg taaagtttca gcgcaacaaa aagaaagtcg
    12601 gcctggtcgt caacgagtat ggagacattc aggggctggt gacggttgaa gatattctgg
    12661 aagagattgt tggcgatttc actacgtcga tgtcgccaac acttgccgaa gaggtcacgc
    12721 cgcaaaacga cggttcggtg attatcgatg gcaccgccaa cgtgcgggaa atcaacaaag
    12781 cctttaactg gcatctaccg gaagatgatg cccgcacggt taatggcgtc attcttgagg
    12841 cactggaaga gatccctgtc gcaggcaccc gcgtgcgtat tggcaagtac gatatcgata
    12901 ttctcgacgt acaggacaat atgattaagc aggtaaaagt ttttcctgtg aaaccgctgc
    12961 gcgagagtgt ggcggagtaa cgaaaacggc cctgcattcg catgcagggc cgtgaattat
    13021 tacgaaaaca gaaattacgc ttttgctttc gctacagtta ccatcgccgc acgaatcgta
    13081 cgaccattca gcgtataacc cttctgcata atgcccagta cgttacctgg cgcaacgtca
    13141 tcagattcca ccattgcgat agcctgatgc acattcgggt ccagcggcac gttagtttcg
    13201 gcgatcactt caacaccaaa cttacgcaca acatccagca tcgacttcag cgtcagctca
    13261 atgccttcaa ccatcgcaga catatccggg ttagctttat cagccacttc cagcgcacga
    13321 tccaggctat caatcaccgg cagcaattcg ttgatgaatt tctccagcgc gaatttgtgg
    13381 gctttttcaa tatccagttc agtacgacga cgcaggtttt ccatttcggc ttttacgcgc
    13441 aaaataccgt cacgttcacg ggtctgggct tcagccagct gagcttcgag attcgcaatt
    13501 ttttcatcgc gcggatccac ctgctcagca gaagcttctg gctcaactgc ctcaatctct
    13561 tcgtgctgat ccatgataat ttcttccggg gcttgcccct caggcgtttt ctgttcttta
    13621 ctactcatga atttctccgc gtttttttcg cattcatctc gctaacttcg cttattatgg
    13681 ggatcagttt cagggtttca agggaagcac tcacattgtc atcaatcttc gcaacaagga
    13741 cctcggaaaa atgaataatc atttcaagtg tattggcatt gtgggacacc cacggcaccc
    13801 tactgcactg acaacacatg aaatgctcta ccgctggctg tgcacaaaag gttacgaggt
    13861 catcgttgag caacaaatcg ctcacgaact gcaactgaag aatgtgaaaa ctggcacgct
    13921 cgcggagatt gggcaactgg ctgatctcgc ggtagtcgtt ggtggcgacg gtaatatgct
    13981 gggcgcggcg cgcacgctcg cccgttacga tattaaagtt attggaatca accgtggcaa
    14041 cctgggtttc ctgactgacc ttgaccccga taacgcccag caacaattag ccgatgtgct
    14101 ggaaggtcac tacatcagcg agaaacgttt tttgctggaa gcgcaagtct gtcagcaaga
    14161 ttgccaaaaa cgcatcagca ccgcgattaa cgaagtggta cttcatcctg gcaaagtggc
    14221 gcatatgata gagttcgaag tgtatatcga cgagatcttt gcgttttctc agcgatctga
    14281 tgggctaatt atttcgacgc caacaggctc caccgcctat tccctctctg cgggcggtcc
    14341 tattctgacc ccctctctgg atgcgattac cctggtgccc atgttcccgc atacgttgtc
    14401 agcacgacca ctggtcataa acagcagcag cacgatccgt ctgcgttttt cgcatcgccg
    14461 taacgacctg gaaatcagtt gcgacagcca gatagcactg ccgattcagg aaggtgaaga
    14521 tgtcctgatt cgtcgctgtg attaccatct gaatctgatt catccgaaag attacagtta
    14581 tttcaataca ttaagcacca agctcggctg gtcaaaaaaa ttattctaat tttacgccag
    14641 cctctttact gtatataaaa ccagtttata ctgtacacaa taacagtaat ggtttttcat
    14701 acaggaaaac gactatgttg gcacaactga ccatcagcaa ctttgctatc gttcgtgagc
    14761 ttgagattga ttttcatagc ggcatgaccg taataactgg cgagaccggt gcgggtaaat
    14821 ctattgcaat agatgccctc ggtctttgtc tcggtggtcg cgctgaagcc gacatggtgc
    14881 gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc tctgaaagat acgccagcgg
    14941 ccctgcgctg gctggaagaa aaccagcttg aagacgggca tgaatgtttg cttcgtcgcg
    15001 tgatcagcag tgatggtcgc tcccgtggtt tcatcaacgg tacagctgtt cctctgtcac
    15061 aactgcgcga actgggtcag ttgctgattc agatccatgg tcagcacgct catcaattac
    15121 tcaccaaacc tgagcaccaa aaattcctgc ttgatggcta tgccaatgaa acctctctac
    15181 ttcaggaaat gaccgcacgt tatcagttgt ggcatcaaag ctgccgtgac ctcgcgcatc
    15241 atcaacagtt aagtcaggaa cgcgccgccc gtgcggaact gctgcaatac caattaaaag
    15301 aacttaacga atttaatccg cagcccggag agtttgagca aatcgacgaa gagtacaaac
    15361 gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa tgcattggca ttaatggccg
    15421 acggtgaaga cgcaaacctg caaagtcagc tttacacggc taaacaactg gtgagcgaat
    15481 tgattggcat ggacagcaaa ctgtccggcg tacttgatat gctggaagaa gctaccatcc
    15541 agattgctga agccagcgac gaactgcgcc actactgcga tcgtctggat ctcgatccca
    15601 accgactgtt tgaacttgaa cagcgcatct caaaacagat ttcgctggca cgtaaacatc
    15661 acgtcagccc tgaggcatta ccacagtatt accagtcgct actggaagaa cagcagcaac
    15721 tggacgatca ggccgactca caagaaacgc tcgcgctggc ggtaacgaaa catcatcagc
    15781 aggcactgga aaccgcgcgc gcattacacc aacaacgcca gcaatatgca gaagaacttg
    15841 cacagctgat caccgacagt atgcatgcgc tctcaatgcc gcatgggcag tttacgattg
    15901 atgtcaaata tgacgagcat cacctgggcg ctgacggtgc cgatcgtatt gagtttcggg
    15961 taaccaccaa cccaggtcag ccaatgcagc ctattgccaa agtcgcatcc ggtggtgaat
    16021 tgtcccgcat cgcactggca atccaggtca tcacggcgcg taaaatggaa accccggcac
    16081 tgatttttga tgaagtggat gtagggatta gcggtccaac agcggcagtt gtcggcaaac
    16141 tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt tacccacctg ccacaagtcg
    16201 cgggatgtgg tcatcaacac tattttgtca gcaaagaaac cgatggtgcg atgacagaaa
    16261 cacatatgca atctctggat aaaaaagcgc ggttacaaga gctggcgcgc ctgcttggtg
    16321 gcagtgaagt cacacgtaac acactggcga atgcgaaaga actgcttgca gcgtaaactt
    16381 ttttcctgct tcacggtcag agtaaacagc aaaacgccgt aagaccggaa agcaaaaggt
    16441 tttaaagtga tgaaaggtct attatcatcg gcatattaca gatgagccac gtactgctcg
    16501 ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc tgactgctgc agcagcagta
    16561 ctattgatgt taaccgcagg ctgttccact ctggagcgag tggtttaccg tcctgacatc
    16621 aaccagggga actatctgac cgctaacgac gtatccaaaa tacgtgttgg catgacgcaa
    16681 caacaagttg cgtacgcatt gggtacaccg ctgatgtccg atccatttgg tacgaatacc
    16741 tggttctatg tcttccgcca gcaaccaggt catgaaggtg taacgcagca aacgctgacg
    16801 ctgaccttta acagtagcgg tgtgttgacc aatattgata acaaacctgc gctgagtggt
    16861 aactaataat aaagttgctc tcaaagacgt taaaaaaggt gctcaatgag cacctttttt
    16921 ctgtctgtta tttattcgct gatttttctg ctcgttgcct gcgaagctct ttcggatcgg
    16981 caatgagagg gcgataaatc tccacccgat cgccatcatg cacactatcg cttagttttg
    17041 ccggacggct gtaaatgccg actttgtttt tagttaaatc gatatcggta cgcaattcca
    17101 gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc ctcctgcagc gtcactcgct
    17161 gcaggtattg cttctcaggt agcgcataag ccacctcaac ggcaatttta cctagcactg
    17221 taaacctctt tcgcacgaac cgtaaaagcc tggaccatat tagccgccag ctctttaaac
    17281 acgcgaccaa aggcgagttc aatcaactta ttggtaaact caaagtcgag atgaaactcg
    17341 atacgacacg cctcctggct cagcggcgta aacttccatc caccaatcaa tttcttgaac
    17401 ggcccatcca ccagattcat aagaatactt tggttactgg tcaactggtt gcgggtagta
    17461 aacgttttgc tgatcccagc cttagagaca tctaccgccg cagtcatctg cccaggagtg
    17521 gactccagaa tccgacttcc ggtacaacct ggcaaaaact gaggataaga ctgaacgtca
    17581 ttcactaact gatacatttg ctccgcgctg tagggtacca gtgcggtccg gctaatctga
    17641 ggcataacaa tttccatcaa caaaaatcca acaaataata tcatttatcc tgctgtaaaa
    17701 aaaacgctat cccggcgctg ggtaacatcg ggttcatgct aagatagagc cttgtccccc
    17761 gcaggattga tatggggtat tttcgatttc agattaccga tgattcacga cgcttatgac
    17821 gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg cttaacaagc gcgcccgtca
    17881 cgaatacttt atcgaagaag agttcgaagc gggacttgcc ctgcaaggct gggaagttaa
    17941 atccctgcgc gcaggaaaag ccaatatcag cgacagctac gtccttctgc gtgacggaga
    18001 ggcatttctg tttggcgcta acatcacgcc aatggccgtg gcctccacgc atgtggtgtg
    18061 cgatcctacc cgtacccgca agttacttct caaccagcgc gaactggact cattgtacgg
    18121 tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc ctgtactgga aaaatgcctg
    18181 gtgcaaagtg aaaatcggcg tcgccaaagg taaaaaacag cacgataaac gttcagatat
    18241 caaagaacgc gaatggcagg tggataaagc acgcatcatg aaaaacgccc accgttaaac
    18301 ctgcactcca attattgacc agttcctcac cgcgcctccc tctccggcgg cgcgaatgaa
    18361 catcttattg gctatcacat ccgacacaaa tgttgccatc ccattgctta atcgaataaa
    18421 aatcaggcta catgggtgct aaatctttaa cgataacgcc attgaggctg gtcatggcgc
    18481 tcataaatct ggtatactta cctttacaca ttggggctga ttctggattc gacgggattt
    18541 gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt aaaaagccgc aaaaaatagt
    18601 cgcaaacgac gaaaactacg ctttagcagc ttaataacct gcttagagcc ctctctccct
    18661 agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat cgcgtggaag
    18721 ccctgcctgg ggttgaagcg ttaaaactta atcaggctag tttgttagtg gcgtgtccgt
    18781 ccgcagctgg caagcgaatg taaagactga ctaagcatgt agtaccgagg atgtaggaat
    18841 ttcggacgcg ggttcaactc ccgccagctc caccaaaatt ctccatcggt gattaccaga
    18901 gtcatccgat gaagtcctaa gagcccgcac ggcgcaagcc ctgcgggctt ttttgtgctc
    18961 cgaatttgtc ccgcgaagtc tgatgccaac taattaaatc cgatcctttt aggcaccttg
    19021 ttaggcactt cataaagcta tattgtcttt gaggtgccta aaactatgga aacccggcaa
    19081 tggcaagaca aaccaaacct ctatccgtta aggaaatcga atctgctaaa cccaaggaag
    19141 cggactacgt tctctatgat ggcgatggcc ttgagctact cattaaatcc agcgggagta
    19201 aaatctggca gtttcgctac attcgccctg tcaccaagac acgagcaaag aaaagcatag
    19261 gcccttaccc gtcagttacc cttgccgatg ccagaaacta ccgtgcagag tcccgctcac
    19321 tcctagctaa acagattgat ccgcaggaac atcagcaaga acaattgcgt agttcgctgg
    19381 aagccaaaac caatactttc cagctcgtag ctgaacgttg gtggaatgtg aagaaagcca
    19441 gtgtgacaga ggactatgcg gaagatatct ggcgctctct tgaaagagat gtctttcctg
    19501 cgattggcga cgttagcgtt acagatatta aagctcatac actggttcag gccgtacaac
    19561 cggttcaggc cagaggagca ctggaaacgg ttcgtcgcct gtgtcaacgt attaatgagg
    19621 tcatgatcta tgcccagaac acagggctga ttgatgctgt tcccagcgtt aatatcggta
    19681 aagccttcga aaagcctcag aaaaagaaca tgcccagcat tcgaccggat cagctatctc
    19741 aactgatgca gacaatgcga acagccagca ttagcctttc cacacgctgc ctgttcatgt
    19801 ggcaacttct tactattacc cgccctgccg aagcggctga agctcgctgg gaagaagtag
    19861 acatagaagc gcaagagtgg aagattcccg cagcacgcat gaaaatgaac cgcgaccata
    19921 ctgttccatt gtcagatgaa gcaatagctg tgctggagat gatgaaaccg ctaagcggca
    19981 accgagaatt tatctttcct agccgtatca agcccaacca gcctatgaac agtcagactg
    20041 ttaacgcctc actaaaacgc gcaggttttg gtggtgtgct tgtttcgcat ggtctgagat
    20101 caattgccag tacggctctt aatgaacaag gctttccgcc tgatgccatt gaggcagcat
    20161 tggcccatgt ggataagaat gaggttcgtc gtgcttataa ccgcagcgat tacctggaac
    20221 agcgtcgccc gatgatgcaa tggtgggcaa attttgtgat ggcagcggat cgtggaagca
    20281 tgattgaagg tgggataaaa ggaatgaagc tagttgggtg actaaatagc tgcgcggcat
    20341 agtgtcccac tttcagccag cccttgcagg ttaagccacc agacagtaaa cggcgggtgt
    20401 cgcgttatta ggcgcagcta tttagtagta atattaggat tggtgattct gcac
//
LOCUS       DAJTPG010000073        20330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTPG010000073 DAJTPG010000000
VERSION     DAJTPG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..122
                     /locus_tag="OO230_002710"
     CDS             <1..122
                     /locus_tag="OO230_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5692407.1"
                     /translation="TPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            94..2004
                     /locus_tag="OO230_002711"
     CDS             94..2004
                     /locus_tag="OO230_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5692408.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2005..2208
                     /locus_tag="OO230_002712"
     CDS             2005..2208
                     /locus_tag="OO230_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5692409.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2205..3798
                     /locus_tag="OO230_002713"
                     /pseudo
     CDS             2205..3798
                     /locus_tag="OO230_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3788..5293
                     /locus_tag="OO230_002714"
     CDS             3788..5293
                     /locus_tag="OO230_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5692410.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5330..5677
                     /locus_tag="OO230_002715"
     CDS             5330..5677
                     /locus_tag="OO230_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5692411.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5735..6763
                     /locus_tag="OO230_002716"
     CDS             5735..6763
                     /locus_tag="OO230_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5692412.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6815..7201
                     /locus_tag="OO230_002717"
     CDS             6815..7201
                     /locus_tag="OO230_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5692413.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7213..7590
                     /locus_tag="OO230_002718"
     CDS             7213..7590
                     /locus_tag="OO230_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5692414.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7577..8161
                     /locus_tag="OO230_002719"
     CDS             7577..8161
                     /locus_tag="OO230_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5692415.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8158..8559
                     /locus_tag="OO230_002720"
     CDS             8158..8559
                     /locus_tag="OO230_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5692416.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8570..9310
                     /locus_tag="OO230_002721"
     CDS             8570..9310
                     /locus_tag="OO230_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5692417.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9369..9755
                     /locus_tag="OO230_002722"
     CDS             9369..9755
                     /locus_tag="OO230_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5692418.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9764..10093
                     /locus_tag="OO230_002723"
     CDS             9764..10093
                     /locus_tag="OO230_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5692419.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10065..13106
                     /locus_tag="OO230_002724"
     CDS             10065..13106
                     /locus_tag="OO230_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5692420.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13106..13435
                     /locus_tag="OO230_002725"
     CDS             13106..13435
                     /locus_tag="OO230_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5692421.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13435..14133
                     /locus_tag="OO230_002726"
     CDS             13435..14133
                     /locus_tag="OO230_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5692422.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14138..14881
                     /locus_tag="OO230_002727"
     CDS             14138..14881
                     /locus_tag="OO230_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5692423.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14818..15420
                     /locus_tag="OO230_002728"
     CDS             14818..15420
                     /locus_tag="OO230_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5692424.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15481..18960
                     /locus_tag="OO230_002729"
     CDS             15481..18960
                     /locus_tag="OO230_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5692425.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            19028..19627
                     /locus_tag="OO230_002730"
     CDS             19028..19627
                     /locus_tag="OO230_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5692426.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19691..>20330
                     /locus_tag="OO230_002731"
     CDS             19691..>20330
                     /locus_tag="OO230_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5692427.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSE"
BASE COUNT         4752 a         4809 c         6435 g         4334 t
ORIGIN      
        1 taacgccgcg tcatattgat catctgaaaa tccttattgc taagggagcg aatcagtgcg
       61 cgcgtgcggg ggataaatta ccggatttac tcgatgaata tatcagaaca acaactgagt
      121 aatatgatgg tggcagtcac tatcgcactg cagccactgg tcagggtatt gccggtgacg
      181 gctgtagaat gggccgatca aaattattac ctgcctaaag aatcgtcata cggtgagggg
      241 gaatggaaaa cgctgcagtt tcaggtcgcc atcatgaact gcatgggcaa tgacgagatt
      301 cgtacagtta acctgattaa atctgcccgt gttggttata caaaaatggt gcttggtgtg
      361 atcgggtatt ttattgagca caaatcccgc aacactcttc tttttcagcc gacggactca
      421 gctgctgaag attttatgaa gtctcacgtg gaagcaacaa ttcgtgatgt tccttgcctg
      481 aaaaaacttt ctccctggct gggccggaaa caccgggata atacgctcac actgaagcgt
      541 ttttcctctg gtgtggggtt ctggtgtttg ggtggtgctg ctgccaaaaa ctaccgtgaa
      601 aaatctgtcg atgtggtctg ctatgacgaa ctctcctcgt ttgaaccgga tgtggaaaaa
      661 gaaggttcgc caacactgct tggcgataaa cgtattgaag gttcggtatg gccaaaatcc
      721 atacgcggtt caacgccaaa aattaaaggc tcctgtcaga ttgaaaaagc tgcgaatgaa
      781 tctgcgcatt tcatgcggtt ttatgtccct tgccctcatt gcggggagga gcagtatctg
      841 aagtttggcg atgatgcgac gtcgtttggc ctgaaatggg agaagggtaa accggaaacg
      901 gtgtattacc tgtgtgaaca taatggctgc gtgatccgtc agtctgaact tgatcagacc
      961 ggggggcgct ggatttgtga caataccggg atgtggacgt gtgacggtct gacgtttttc
     1021 agtgcttcgg gtaatgaaat tccgccgccg cgctccatca ctttccatat atggacggcg
     1081 tacagtccgt tcaccaccag ggtacagata gtctgtgact ggctggatgc actgaaagat
     1141 cccaacggcg tgaaaacttt tgtgaacacc acgctgggcg agacctggga agaggccgtg
     1201 ggcgaaaaac tcgatcacca ggtactgatg gataaggttg tgccttacac ggcaacggtg
     1261 cctgtccggg tggtttatct gacggcgggc attgactcgc agcgaaaccg ttttgagatg
     1321 tatgtctggg ggtgggctcc gggagaggaa gcctttctgg tggataaaat catcatcatg
     1381 ggacgtcctg atgaggaaga gacgctgtta cgtgtggatg cggcgatcaa taaaaaatac
     1441 cgccatgcag acggtaccga aatgaccatt tcccgtgtct gctgggatat cggggggatc
     1501 gatggcgaaa tcgtgtatca gaggtcaaaa aaacacggta ttttccgggt actgccggta
     1561 aaaggtgcct ctgtctatgg caagccggtg atcaccatgc cgaaaacccg caatcagcgg
     1621 ggtgtgtatc tgtgtgaggt ggggacggat accgcaaaag aaattctcta tgcccgaatg
     1681 aaggccgatc ccacccctgt gaatgaggcc acatcgtatg ccatccgctt tcctgataat
     1741 ccggagattt tttcgcagac agaggcacaa caactggtgg cggaggaact ggtggaaaag
     1801 tgggaaaaag gaaagatgcg tctgttgtgg gataacaaaa agcggcgtaa cgaagcgctg
     1861 gactgcctgg tgtatgccta tgcggcatta cgtgtgtccg tgcaacgctg gcaacttgat
     1921 ctggctgtgc agttgcatcc cgcaaggatg caactgcaca accgaccctt gaagaactgg
     1981 cagcgaagct gtccggagga gtgaatggtt acagtcgctg aactgcaggc gctgcgtcag
     2041 gcgcgccttg atttattaac cggtaaacgg gttgtgtcgg tacagaaaga cggtcgcagg
     2101 atcgaatata cggcagcgtc cctgaatgag cttaaccgtg cgatcaacga tgcggagtcg
     2161 gttctgggta cgacccgacg ccgccgaccg ctgggagtga ggttatgaaa cgaacgcctg
     2221 tcctgattga tgtgaacggc gttccgcttc gtgagagtct cagctacaac ggaggcggcg
     2281 caggatttgg cgggcaaatg gcagagtggt taccaccggc gcagagtgcc gatgcggccc
     2341 tgttgcctgc attgcgtctg gggaatgccc gggcagatga tctggtgcgc aataacggta
     2401 tcgcggctaa tgcggtggcc ctgcataagg atcatatcgt cgggcacatg tttcttatca
     2461 gctaccgtct gaactggcgt tggctgggga tgcgggagac cacggcaaaa agttttgtcg
     2521 gggaggtgga agcagcctgg tcggaatacg cagaaggaat gtttggcgag atcgacgtgg
     2581 aagggaaacg cacgtttacg gaatttatcc gtgaaggggt gggcgtgcat gcctttaacg
     2641 gcgaaatctt tgtgcagccg gtctgggata cggaaaccac gcagttattc cgtacgcgtt
     2701 ttaaagccgt gagtccgaaa cgggtggaca cgccaggaca cggtatgggg aaccactttc
     2761 tgcgggccgg ggttgaggtc gatcggtatg ggcgggcgat tgcgtaccat atctgtgagg
     2821 atgattttcc gttctctggt gccggacgct gggagcggat cccgcgtgag cttcccaccg
     2881 ggcgtccggc catgctgcat atttttgagc cggtggagga cgggcagacc cgtggggcta
     2941 atcagtttta cagcgtaatg gagcggatga agatgctgga ctccctgcag gccacgcagc
     3001 ttcagtcggc cattgtgaag gcgatgtatg cagccacaat cgaaagtgaa cttgataccg
     3061 aaaaggcatt tgaatatatc gcgggggcac cgcaaggtca gcaggataat ccgcttatta
     3121 atattctgga gaagttcacc cgctggtatg acacgaataa cgtgacgctg ggcggtgtta
     3181 aaattccgca ccttttttcc tggcgatgcg ctgaacctgc agacagcgca ggattcagac
     3241 aatggatttt cggcgcttga acaggcgctg ctgcggtata ttgcagccgg tcttggcgtt
     3301 tcctacgaac agttgtcccg tgattactcg aaggtcagtt actcaagtgc ccgcgcctcc
     3361 gccaatgagt cgtggcgcta ttttatgggg cggcgaaaat ttattgcggc ccggctggcc
     3421 acgcagatgt tttcctgctg gctggaagag gcgcttcttc gggggattat tcgtccgcca
     3481 cgggcgcgtt ttgattttta ccaggcgcgt tcagcctggt cacgggcaga gtggattagc
     3541 tccggaagaa tggccattga cgggctcaag gaggtccagg aatcggtgat gcgcattgag
     3601 gccggactga gcacgtatga gaaagaactg gcactgatgg gcgaagatta tcaggacatt
     3661 ttccgccagc aggtcaggga atccgcagag cgcgaaaaag ccggactctc acgtccggtg
     3721 tggatagccc aggcgtatca gcagcagata gcggagagtc gcaggccgga agaggagaca
     3781 acatcacgtg agacgtaatc tttcacatat tatcgctgcg gcattcaatg agccgctgct
     3841 tctggagccc gcctatgcgc gggttttctt ttgcgcgctc gggcgtgaga tgggcgcagc
     3901 aagcctttcg gtaccgcaac agcaggtaca gcttgacgct cccgggatgc tggctgaaac
     3961 ggacgagtac atggccggag gcaaacgacc tgcccgtgtt taccgggtgg tgaacggtat
     4021 tgctgtactg ccggtgaccg gcacgctggt acaccggctg ggtggcatgc ggccattttc
     4081 cggaatgact ggctatgacg gcattgtcgc ctgtcttcag caggcaatgg cggacactca
     4141 ggtacggggc gtactgctgg acattgacag ttctggcggg caggccaccg gcgcgtttga
     4201 ctgcgctgac atgatttacc gtctccggca gctgaagcct gtctgggcac tgtgcaatga
     4261 cacggcctgt tcagccgcca tgttactggc atcggcctgc tcccgacggc tggttaccca
     4321 gacatcccgt atcggctcca ttggggtgat gatgggccat gtcagctatg ccggtcatct
     4381 ggcacaggcc ggtgtggata tcacgctgat ttacgccgga tcgcacaagg tggatggcaa
     4441 tcagtttgaa tccctgccag caaaggttcg ccaggacatg cagcagcgca ttgatgcggc
     4501 gcgccggatg tttgctgaaa aagtggcgat gtataccggt ttatctgtgg atgcggttat
     4561 gggaacagag gccgccgttt ttgaaggtca gtccggtatt gaggccgaac tggcggatga
     4621 attaatcaat gcgtcggacg ccatcagcgt gatggctgcg acgctgaaca cacacgatac
     4681 aggaggcact atgccgcaat taactgcaac ggaggctgcc gcgcaggaga accagcgagt
     4741 gatggggatc ctgacgtgtc aggaagcgaa aggacgtgaa caactggcca cgatgctggc
     4801 agggcaacag ggtatgagcg ttgagcaggc ccgggcgatt ctggccgtgg cggcaccgca
     4861 acaatctggt gtatctgcgc agagtgaagc cgatcgcatt atggcatgtg aagaggcgaa
     4921 aggacgtgaa caactggcgg caacgctggc ggcgatgccg gagatgacgg tggaaaaagc
     4981 ccgtccgatt ctggctgctt caccgcaggc gaatgccgga ccctcacttc gtgatcagat
     5041 tatggccctg gatgaggcaa aaggggcaga ggcgcaggct gaaaaactgg cggcctgtcc
     5101 ggggatgacc gtggagaatg cccgggctgt actggcggca gcctcaggca gggcagaacc
     5161 ggtttctgca tccacaaccg ccatgtttga acatttcatg gcgaaccatt caccggcagc
     5221 ggtacagggt ggcgtgccac agacgtcagc agacggtgat gcggacgtga aaatgctcat
     5281 ggccatgcca tgaagtcagt gctgaccatc aatatgaggt ttttacaata tggtaacgaa
     5341 aaccatcact gaacagcgtg cggaagtacg tatttttgcc ggtaatgatc cggcccatac
     5401 cgccacaggc tgcagcggga tttcctcggc aacaccggct ctgacgcccc tgatgctgga
     5461 tgaggatacc gggaaactgg tggtctggga cggacagaag gcaggccatg tggttggcat
     5521 actggtactg ccgcttgaag gtacagagac ggtgctgacg tattacaagt cggggacctt
     5581 tgcgacggag gccatccact ggcctgagag agtggataca cacaaaaagg cgaatgcctt
     5641 tgccggcagt gccctgagtc acgcagcgct gccgtaacat tttatcaggt cactgcggta
     5701 gcctgactga tttctgaatg aaaggaactg atttatggga ttgtttacga cccgccagtt
     5761 actcggttat accgaacaaa aagttaaatt tcgtgcgctg tttctggagc tgtttttccg
     5821 ccgtacggtg aatttccata ccgaagaggt aatgctggac aaaattaccg gaaaaacgcc
     5881 ggtggcggcc tatgtctccc cggttgttga aggaaaggtg ttgcgtcacc gtggcggtga
     5941 aactcgtgta ctgcgcccgg gctacgttaa gccgaaacac gaatttaatt accagcaggc
     6001 ggtggagcgt cttcccggtg aagatccggc ccagctgaac ggcccggctt accgtcgcct
     6061 gcgtatcatc accgataacc tcaaacagga agagcacgcc attgtccagg tggaagagat
     6121 gcaggcggtg aatgccgtgc tgtatggcaa atacaccatg gaaggagagc agtttgaaac
     6181 tgttgaggtc gattttggcc ggtcggtggg aaataacatt acgcaggcag gagggacgga
     6241 gtggagcgct caggaccgcg atacctttga tccgacgcat gatctggatg cgtactgtga
     6301 ttttgcttcc ggcaccatca atattgccat tatggatggt aaggtctggc gtctgctgaa
     6361 tggctttaag ctgttccgcg aaaaactgga tacccgtcgt ggctcaaatt cacagctgga
     6421 aacggcagtg aaagacctgg gggcggtggt gtccttcaaa gggtattatg gtgatctggc
     6481 aatcgtggtg gcaaaaacgt cctatgtggc agaggacggt accgaaaaac gttatctgcc
     6541 ggacggcaca ctggtactgg gaaatacggc cgcagagggt attcgttgct acggtgccat
     6601 tcaggatgcg caggcattgt ctgaaggcgt ggtggcgtct tcccgttatc cgaaacactg
     6661 gctgaccgtg ggtgatccgg cgcgtgaatt caccatgacg cagtccgcgc cgctgatggt
     6721 gctgccggat ccggatgagt ttgtggtggt gcaggtgaaa taatccgtga gcgggagcta
     6781 aatgctcccg tttttcattc aatcaggagc gggtatggct acgaaagaag aaaacctgaa
     6841 acgtctttgt gagctggctg agaggctggg gcgtgaaccg gatgtctccg gtagtgcggc
     6901 agatattgcg cagcgagtgg cggaactgga ggaagagctt ggagatgcgg gggaggtcgc
     6961 agagccggat acgtactcac catcgaaaga tgatccgacc gcgcataaag gcgagtcagt
     7021 tcctgaacag ccaggcattg ttaccggaga cgaaacgggg caggttacgg tggtggcact
     7081 ggcgacactg catacaaaat gtaatggttc agtgatattt gtttcaccgg gaacgtcgtt
     7141 tcgtgtttct gctgaggtgg ctgccagcat ggcggcaggt ggtctggcaa aaaggcagta
     7201 acaggagatg tcgtggacgg tttcgataat ctgtttgatg ctgcgcttgc cggtgtcaat
     7261 gaagtcattc tccgtgatat ggggatcagc gccgtcatta cgtccggaga acttgagggg
     7321 acacacctga caggggtttt tgatgatccc gaaagtattt cttttgttgc cggggggatc
     7381 cggcttgagg attcctcacc gtgcctgttt gtgaaaacgg cagatatatc gcagttacgc
     7441 cgtcaggata cgttaactat cggggatgat tctttctttg tggatcgtat tactccggat
     7501 gacgggggat gctgttatat ccggttaagg cggggcagtc ctgccccaca gaataatgcc
     7561 agaatgaggc gatatgatga agggacttga gaatgccatc cgcaatctga acagcctcga
     7621 tacccgcatg gtgccacaga ccagcgcatg ggcggtaaac cgtgtggcgg caaagattgt
     7681 ttctgtcgcc acacggcagg ttgcgcagaa taccgttgcc ggggataacc aggtgaaagg
     7741 tattcccctg aaaacggtca gggagcgtgt caggctgctt aaagccagcc cctcaggaaa
     7801 aatgtatgcc cgcatgcgtg ttaaccgggg taacctgccc gccattaagc tgggtaccgc
     7861 acaggtcaga ctggcccgct cccggcatgg cagcaactca cgtcatcgtg gcagcgtact
     7921 gaaagtgggg aaataccttt tccgggatgc gtttattcag caactggcga atggtcgctg
     7981 gcatgtgatg cggcgtattg acggcaaaaa tcgttacccc attgatgtgg tgaaaatccc
     8041 gatgtccgga ccgctgacac aggcatttga agatgcccgc gaccgcatca ttgctgcgga
     8101 aatgccgaaa cagctggggt atgccctgaa acaacaactg aggttacatc tgagtaaatg
     8161 aaccgacaca cacaaatccg tcagagcgtg ctggcacgcc ttcgggaaca gtgtggagac
     8221 agcgccacgt tttttgacgg gcttccggca tttattgatg cgcaggaact gcccgccgtg
     8281 gcggtgtggc tgagtgatgc gcagtacacc ggaaaaatga cggatgagga cgactggcag
     8341 gctgttctgc atattgccgt tttcgtccgt gcacaggcgc cggattctga gctggatacg
     8401 tggatggaga acaccatatt tccggccatg aaggatattc cggtgctttc cggactcatc
     8461 gacaccatga tcccactcgg ttttaactat caacgtgata atgagatggc cacctgggcg
     8521 atggcggaaa tcacgtacca gatcacgtac acgaattaag gaggtggtaa tgaccacacc
     8581 aaatccactg gcaaagacga aaggtgcggg gacgacgttc tggatgtata ccggcaacgg
     8641 cgatgcgttt gcgaaccctt tgtcggacac tgactggctg cgtcttgcga tggtgaagga
     8701 tctgcaacct ggcgaaatga ccgctgatgc agaagatgac acttatctcg atgatgaaga
     8761 tgcagactgg aaaacgacaa cccaggggca gaaatccgtt ggtgatactt cggcgacgct
     8821 ggcctggcgt ccgggtgaca gcgggcagaa aaaactggtt cagttgttcg actccggtga
     8881 agtctgcgcg tttcgtatca aatatcccaa cggcactgtt gatgttttcc gcggctggct
     8941 gagctcactg ggtaaaacca ttgcctcaaa agacgtgatg acccgcactg tgaaaatcag
     9001 cggtgtgggg cgtccgtatc tggcagagga aggcactgaa accgtgggtg ttaccggact
     9061 gacggtggca ccggcatctg tcagtgtcaa agcgggagca accaccacac tgacctttac
     9121 agtaaaacct gacggggcca gtgacaaagc gatcagtgtg cattcgtcag atcctcagac
     9181 tgcttcggtg accctgagcg ggcttgtggc cacggtgaaa ggcgtgaagc agggcagtgt
     9241 cagcatcgtg ggcatgacct ctgacggaga gtttgtggca gtggctgcgg tgaccgtcag
     9301 cgcaccataa caggacgata ctcatcattg ccccggttat ccggggcttt ttgcatccgg
     9361 agaacatgat gtttctgaaa caggatacgt ttaattatga aaaacagtcc gtggtgctca
     9421 gtgagctgtc cgggctgcag agaattgaat atctgacgtt tgttcagcag cgaacggcaa
     9481 agtttgatgc acaggaggga gaactgccgg aggctgaacg acagattgct tttctgcgta
     9541 tgggaatgga tatcaatgcc tggctggttt cccgctcact gtggaatgct gagcagtctc
     9601 aggatgttga gacgctttgc gcatccatta tgacaacatg gtcgtatgat gcgctgggcg
     9661 cgggggcgga gagggttctg tcgctgagcg gtatggggac cattgagaat gccggggatg
     9721 atgatcatga ggcgctgacg ccggaaaagt cctgacgcgg gaaatgcagt ttgtcatgcg
     9781 gcttgcccgg gagttccggc gggcagactg gcggcggatg ctgtcggata tgtcggccac
     9841 tgagcttggt gagtggggcg attatttccg gatgcagagc ttcagtgatg tgtggatgga
     9901 tgcgcagttt gcctcgctga aggcattgat cgtgagaatg gtgtccggca gcagcgatgc
     9961 tgcggtggct gatttcagcc ttttaccgga agagaacggg ataccggagc gaacggacga
    10021 agaactgatg catcttgggg aaggtatttc cggaggtgtg cgttatggac cagatagcca
    10081 acctggtcat tgatttgggg attgatgcgg cagagtttaa aaatgaaatt ccccgtatca
    10141 aaaaccttct gaatggtgca gccagcgatg cagaacggtc ttctgcccgt atgcagcgtt
    10201 ttatggagcg tcagactcag gcggcccggc agacaatgca ggcggcttct tcggctgcaa
    10261 cagcagcatc agcccatgcg cagacggtgg agaagaatgc acgggctcat gaacgcatgg
    10321 cccgtgaggt ggaacaaacc cgtctgcgcg tggatgccct gaatcagaaa atgcgcgagg
    10381 aacaggcgca ggccagggca ctggcggagg cgcaggataa agcggctgcc gccttttatc
    10441 gccagattga cagtgtgaaa caggccggtg cggggcttca ggaattacag cgtattcagc
    10501 agcagatccg acaggccaga aacagtggcg gggttggtca gcaggattat ctggcgctga
    10561 tttcggagat cacggcgaaa acccgtgccc tgacgcaggc agaggaacag gccacccggc
    10621 agaaagcggc gtttatccgc cagcttaaag agcaggcaac ccgccagaat ctgtcgtctt
    10681 ctgagttgct tcgcgccagg gcggctcagc tgggggtaag cagtgctgca gaagtgtata
    10741 tccgcaaaat ggagcgggca ggaaaagcca cacattcgct ggggctgaaa agtgcggcag
    10801 cccggcggga gctgggggtg ttaatcagtc agatggcgcg aggcaatttt ggtgcgctga
    10861 ggggatccgg gataacgctg gctaaccgtg ccggatggac aggcgcactg atgtcgccga
    10921 aaggcatgat gactggcggc gttattggcg gacttgtcgc ggcggtcctg ggtctgggta
    10981 aagcctggca tgacggccgg aaggagggcg agaaatttaa ccgtcagctg gcgctgacgg
    11041 gacattatgc cggtgtcact gtcgggcagt tgtggaaact cagccgggcc atatccggga
    11101 atggtatcac gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc agtggggcat
    11161 ttcatggaaa cgatattggt atggtggcga aagctgccgc acagatggag cgatcggttg
    11221 gccagtcggt cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg
    11281 ccgcgaaggc gctggacaat gcgctgcatt ttttgaatgc cactcagctt gagcagatac
    11341 gcgtccttgg ggagcagggg cggtccagtg atgcggcccg gatcgccatg tctgcgctgg
    11401 cagaggaaac cggtaaacgc acatctgata ttgataataa tctcaatgcg ctgggtagta
    11461 cgctgcaaac cttgtctgac tggtggaagc agttctggga tgcggccatg aatattggtc
    11521 gtgaagactc gctggatgcg cagattgatg cgttacagga aaaaattcag cgcgcgaaaa
    11581 aatatccgtg gacaaacgcc tccacacagg tggagtacga tcagcagcgt cttaacgatc
    11641 ttcaggagaa aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca gaacggaatt
    11701 accaggagca acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg atgaatgaaa
    11761 cggaagctgc acgacatcag cgggaaattg cgcgtattaa tgccatgcag tacgccgacc
    11821 aggctgtcag ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa gccattaaga
    11881 aaaatacacg ggcaacccgt aatgatgagg ccacccggtt attgctgcag tacagtcagc
    11941 agcaggcaca ggtggaaggg cagattgctg ctgccagaca gtcagcaggc attgccactg
    12001 aaaggatgac tgaagcgcat aaacagcttc tggccctgca gcagcgcatc agcgacctgg
    12061 acgggaaaaa gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat gagctgattc
    12121 aggcgctgac gctgctggat gtgaaacagc aggagctgca gaaacagaca gcgcttaacg
    12181 acctgagaaa aaaaacggtt cagctgacca gccagctggc agacaaagaa cgtgcactgc
    12241 gtgagcagca caatctggat attgccactg caggtatggg ggataagcag cggcagcgct
    12301 accaggcaca gttgcgcatc cggcaggaat accggcaaca gttgcaacag cttgagaatg
    12361 acagtcgcca gaaaggcact tacgggacgg aggactaccg gagggctgag gaggtgctga
    12421 aggggagcct gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa ctggaagtgg
    12481 cgcagggcga ctggaaaaac ggtgccatgc gggcgtttca gaattttacg gcggatgcgg
    12541 ataatgcggc gggaacggca gaacagatgt ttacagtggc attcagcagt gccggtaatg
    12601 cactggcgac attctgtacc accggtaagc tgaattttaa atccttcacc tcttccctgt
    12661 tgtcagatat ggccagaatt atggcacaga tggccatgat gcaggcggta aagggcgtcg
    12721 gttctttatt cggcttcacg actaatgctg atggcggtgt ttaccagtct gctgatttga
    12781 gtcgctacag tggcacggtg gttaaccgtc cgacgttttt tgcttttgca aaaggcgcgg
    12841 gtgtgatggg ggaagcggga cctgaagcca ttctgccact gcgtcgcggt gctgatggta
    12901 agctgggggt tgtggcggat actggtggtt caggcatggt gatgtttgcc ccgcagtaca
    12961 acattgagat caataacgac ggcacgaacg ggcagatagg tccggctgcc ctgaaggtgg
    13021 tttatgacct cgggaaaaaa gcggccgcgg actttatgca acagcaggcc cgtgatggtg
    13081 gccggttaag tggagcatac cggtaatgga gacgtttcac tggaaagtgc gcccggatat
    13141 gaatgtggta tcagagccga aagtggtgac agtgaagctg ggcgatggtt atgaacagcg
    13201 tcgtgcggcg ggactgaata accagttgtc gacttacagc gtgacgatac gtgttcgtaa
    13261 aggtgaacac ccatctttaa aagcctttct ggaacggcac ggtggcgtcc gcgcatttca
    13321 gtggacgcca ccttatgact ggaaactgat cagggtggtt tgtcgtaaat ggtcggcaag
    13381 cgtgggggcg ttatgggtga ctgtaacggc cgattttgaa caggtggtga actgatgcag
    13441 gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcagc ccgggtggag
    13501 ctctgggaaa tcgatctgac agaggtcggt ggtgagcgtt attttttctg taatgagcag
    13561 aacgaaaaag gtgagccggt cacctggcag gggcggcagt atcaggcata ccccattcag
    13621 gggtcggggt ttgaactgaa cggcaggggc tgtgctgccc gtccgacact gacggtctct
    13681 aacctgcacg gcatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg
    13741 gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac
    13801 agcgacgccg atccggaaca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa
    13861 ctgagcgcgg tcagtgcctc ttttgtgctg gccacaccaa cggagacgga tggcgcggtt
    13921 ttcccggggc gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc tgatgagtgt
    13981 ggttacacgg gcagggcagt ggctgacgag ttcgacaaac caacaacgga tatccggaag
    14041 gacaaatgca gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg taatttcggc
    14101 ggtttccttt ccatcaataa actttctcag taaatccatg acacagacag aatcagcgat
    14161 tctggcgcac gcccggcgat gtgcgccagc ggagtcgtgt ggcttcgtgg tgagaacgcc
    14221 ggagggagac aggtatcttc ccagcgagaa tatctccggt gagccggagg aacggttccg
    14281 gatggctccg gaggactggc tgcgggcaca aatgcagggt gagattgtgg cactggtcca
    14341 cagtcatccc ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag
    14401 tgatttgccg tggtggctgg tctgccgggg gacgattcac aagttccgct gtgtgccgca
    14461 tctcaccggg cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc
    14521 ttatcatctg gcggggattg agatgccgga ttttcatcgc ggggatgact ggtggcgtca
    14581 cggtcagaat ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc
    14641 atcagcacaa ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc
    14701 cgccatttac tgcggcgatg gtgagctgct gcaccatatt cctgaacaac tgagtaaacg
    14761 agagaggtat accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggaatg
    14821 gcacgcatct gcctttacgg ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg
    14881 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggcgtt tcgtcagaaa
    14941 ctgagtgacg gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga aaccgaattg
    15001 tctgcccgtc ttaatgagcc gctggcaaat ggtgcagtga tccacatagt accgcgtctg
    15061 gtgggagcta aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt
    15121 gctatatgga tgccgggggt aggaattatg gcgagtaatc tgctgttttc tctcggtgcc
    15181 agtatgacgc ttggcggtgt tgcacagatg ctggcaccga aagccagaac tccccgtaca
    15241 cagacaacgg ataacggcaa acagaacacc tgtttctcct cactggataa catggttgcc
    15301 cagggcaatg ttctgcctgt tctgtacggt gaaatgcgcg tggggtcgcg ggtggtatct
    15361 caggagatca gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga
    15421 tgcaaaatgt ttcatgtgaa accgcctgcg ggcggttttg tcgtttatgg agcgtgagga
    15481 atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaaatcc
    15541 acgcagttgc tgagtgtgat tgatgccatc agcgaagggc cggttgacgg tccggtggat
    15601 ggattaaaaa gcgtgctgct gaacggtacg ccggtcctgg atagcgaggg gaagacaaac
    15661 ttttccggtg ttacggtggt gttccgcgcc ggcgagcagg agcagacacc gccggagggg
    15721 tttgaatctt ccggctcaga gactgtgctg ggtacggaag tgaaatacga cacgccgatc
    15781 acccggacca tcacgtcggc aaacattgat cgtctgcgcc tgaccttcgg tgtgcaggca
    15841 ctggtggaaa ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct gctggttcag
    15901 atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccact
    15961 tcgcagtatc tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt taatatccgg
    16021 atgcgcagga tgacaccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg
    16081 tcgtacaccg aaatcatcga tgtgaaacag tgctacccga acacggcgct ggtcggcgtg
    16141 caggtggatt cagagcagtt cggtaaccag caggtgagtc gcaattatca tcttcgcggg
    16201 cgcattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg
    16261 gacggaacgc ttaagccagc atacagcaac aacatggcct ggtgtctgtg ggacatgctc
    16321 actcatccgc gctacggcat ggggaaacgt cttggtgcgg cagatgtgga caaatgggcg
    16381 ctgtatgtca tcggccagta ctgcgaccag tcagtgccgg acggatttgg cggcacggag
    16441 ccgcgcatca cctgtaacgc ttacctgacc acacagcgta aggcgtggga tgttctcagt
    16501 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg
    16561 caggaccgac cgtcggataa agtgtggacc tataaccgca gtaatgtggt gatgccggat
    16621 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgtcgttgag
    16681 gtgaactgga ttgatccgga caacggtcat gagacggcga cagagcttgt ggaggacacg
    16741 caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc
    16801 cggggacagg cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag
    16861 accgtggact tcagcgtcgg cgcagaaggg cttcgccatg taccgggtga tgtcattgaa
    16921 atctgcgatg atgattatgc cggtatcagc accggtggtc gcgtactggc ggtgaacagc
    16981 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg taccacgctg
    17041 ataagcctgg ttgacggaaa tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac
    17101 ggcgtgaagg tgaaagtgag ccgggttcct gacggcgttg cagaatacag cgtgtggggg
    17161 ctgaagctgc cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg tgagaacgac
    17221 gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat
    17281 aacggggcac actttgacgg tgaccggcgc ggcacggtga atggtgtcac gccgccagcg
    17341 gtgcagcatc tgaccgccga agtcaccgca gacagcgggg aatatcaggt gctggcgcga
    17401 tgggacacgc cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac cgtggcagcg
    17461 gacgacggca gtgagcggct ggtcagcaca gccaggacga cggaaaccac ataccgcttc
    17521 aggcagctgg cgctggggaa ttacagtctg acagtccggg cggtaaatgc ccgggggcag
    17581 cagggcgatc cggcgtcggt atcgttccgg attgcggcac cggcagcgcc tgtcactatt
    17641 gaactgatac cggggtattt tcagataacg gtggtcccga aactggctgt atatgacccg
    17701 acggtgcagt ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa
    17761 accagcgccc gctatcttgg cacggcgctg tactggatag ccgccagtat caatatcagg
    17821 ccgggccatg attattattt ttacgttcgc agtgtgaaca ccgttggcaa atcggcattt
    17881 gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt atctgagttt ttataaaggg
    17941 ttgatcaata aaacgcatct cggcaaggag ttgtggacgc agattgataa cggtcagctt
    18001 gcgccggacc tgactgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcaa
    18061 accgtcaata aaaaactgga aaatcagagt gctgcaatcc agcagataca gaaagttcag
    18121 gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcaaca gatgaaggac
    18181 ggacgccttt atattgcggg tatcggtgcc ggtattgaga atacgccagc aggaatgcag
    18241 agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa tggcaacaca
    18301 aagccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt cctgaaacgc
    18361 ctgacggctc cgaccattac cagcggcggt aatcctccgg cattttccct gacacctggc
    18421 ggacggctga cggcgaaaaa tgccgatatc agcggtaacg tgaacgcgaa ctccgggacg
    18481 ctcaacaacg tcacgattaa taagaactgt cgggttctgg gaaaattgtc cgcgaaccag
    18541 attgaaggcg atctcgttaa aacagtgggc aaacctttcc cacgggactc ccgggcaccg
    18601 gagaggtggc catcagggac cattaccgtc agggtttatg acgatcagcc gtttgatcgg
    18661 caaattgtta ttcccgcggt ggcgtttcgc ggtgctaaac atgagcggaa gaataacaat
    18721 atttattcgt catgccgcct gatagtgaag aaaaacggtg ctgaaattta taaccgaacg
    18781 accctggata atacgctgat atatacgggt gttattgata tgcctgccgg tcacggtcac
    18841 atgacgctgg agttttctgt atcggcatgg ctggtaaatg gctggtatcc cacagcaagt
    18901 atcagcgatt tgctggttgt tgtgatgaag aaatccacag caggtatcag tatcagctga
    18961 attttataac ccagatacgg gcaccagaaa tggtgccttt tttattgcag aaaagcgaga
    19021 ggtaattatg cgtaaacttt atgctgccat tttgtccgca gccatctgtc tggctgtatc
    19081 cggtgcgcct gcatgggcgt ctgaacatcg gtccacgctg agcgcggggt atcttcatgc
    19141 ctcgacgaac gctcccggca gtgatgatct gaacgggatt aacgtgaaat accgttatga
    19201 gtttacggac acgctggggc tggtgacgtc attcagctat gccaacgctg aagatgagca
    19261 aaaaacgcat tacagtgata cccgctggca tgaggattcc gtgcgtaacc gctggttcag
    19321 cgtgatggcg gggctgtctg tgcgcgtgaa tgaatggttc agcgcgtatg cgatggcggg
    19381 cgtggcttac agccgtgtgt cgaccttctc cggggattat ctccgcgtaa ctgacaacaa
    19441 ggggaaaacg cacgatgtgc tgaccgggag tgatgacaac cgccacagca acacgtctct
    19501 ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc gtggccattg accttgctta
    19561 tgaaggttcc ggcagtggcg actggcgcac tgacggtttc atcgtgggtg tcggttataa
    19621 gttctgatta gccaggtaac acagtgttat gacagcccgc tggttcaggc gggctttttg
    19681 tggggtgaat atggcagtaa agatttcagg tgtactgaaa gatggggcgg gtaaacctgt
    19741 cgtaaattgt gcgattgaac tgcgggccag aagaaccagt ccgaccgttg tggcacacgt
    19801 tgttgccact tgcgtgacgg acaataacgg tgcttatgtg attgaggctg agccggggta
    19861 ttacgaggtt gcgcttcact gtaacggctg gcagccaacc cgtgtcgggg atattgatgt
    19921 ggcaccgact gatgcaccgg ggacactgaa cgcgtttctg aatgcaccaa aggatggtga
    19981 tttacgtccg gaggtgatga agcgctttga ggaaatggtg gcgcaggcgc agcagagtgc
    20041 cggggctgca gccggaaacg cacagcagac ggcgcaggat gtggcggcag ccgcaacggc
    20101 ccgtgatgat gcacaacgtt ttgcggagaa agcccgacag gatgcaaccg tcacagctga
    20161 ggacagaaag gccactgcgg aagatgtgac aagcacagga gcaaatgcag ccgcagccgg
    20221 acagagcgca caggatgccg caggttatgc ccgcgcagca gaacaggcca aaaatgacat
    20281 tgatgctgcg ctgaccggca ctctgaaaat ggctaaccat ctgtcagaaa
//
LOCUS       DAJTPG010000074        20279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTPG010000074 DAJTPG010000000
VERSION     DAJTPG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(51..809)
                     /gene="aroD"
                     /locus_tag="OO230_002732"
     CDS             complement(51..809)
                     /gene="aroD"
                     /locus_tag="OO230_002732"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5692428.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(840..1706)
                     /gene="ydiB"
                     /locus_tag="OO230_002733"
     CDS             complement(840..1706)
                     /gene="ydiB"
                     /locus_tag="OO230_002733"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5692429.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1718..2989)
                     /locus_tag="OO230_002734"
     CDS             complement(1718..2989)
                     /locus_tag="OO230_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692430.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3172..4377)
                     /locus_tag="OO230_002735"
     CDS             complement(3172..4377)
                     /locus_tag="OO230_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692431.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4477..4833)
                     /locus_tag="OO230_002736"
     CDS             complement(4477..4833)
                     /locus_tag="OO230_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5692432.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4969..5076)
                     /gene="rprA"
                     /locus_tag="OO230_002737"
     ncRNA           complement(4969..5076)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO230_002737"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5262..6374)
                     /gene="ydiK"
                     /locus_tag="OO230_002738"
     CDS             complement(5262..6374)
                     /gene="ydiK"
                     /locus_tag="OO230_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5692433.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6763..9819
                     /locus_tag="OO230_002739"
     CDS             6763..9819
                     /locus_tag="OO230_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5692434.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9816..10226
                     /gene="menI"
                     /locus_tag="OO230_002740"
     CDS             9816..10226
                     /gene="menI"
                     /locus_tag="OO230_002740"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5692435.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10326..10514
                     /locus_tag="OO230_002741"
     CDS             10326..10514
                     /locus_tag="OO230_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5692436.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11062..11430
                     /gene="sufA"
                     /locus_tag="OO230_002742"
     CDS             11062..11430
                     /gene="sufA"
                     /locus_tag="OO230_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5692437.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11439..12926
                     /gene="sufB"
                     /locus_tag="OO230_002743"
     CDS             11439..12926
                     /gene="sufB"
                     /locus_tag="OO230_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5692438.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12936..13682
                     /gene="sufC"
                     /locus_tag="OO230_002744"
     CDS             12936..13682
                     /gene="sufC"
                     /locus_tag="OO230_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5692439.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13657..14928
                     /gene="sufD"
                     /locus_tag="OO230_002745"
     CDS             13657..14928
                     /gene="sufD"
                     /locus_tag="OO230_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5692440.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14925..16145
                     /gene="sufS"
                     /locus_tag="OO230_002746"
     CDS             14925..16145
                     /gene="sufS"
                     /locus_tag="OO230_002746"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5692441.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16158..16574
                     /gene="sufE"
                     /locus_tag="OO230_002747"
     CDS             16158..16574
                     /gene="sufE"
                     /locus_tag="OO230_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5692442.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16723..17727
                     /gene="ldtE"
                     /locus_tag="OO230_002748"
     CDS             16723..17727
                     /gene="ldtE"
                     /locus_tag="OO230_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5692443.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17790..18026)
                     /gene="lpp"
                     /locus_tag="OO230_002749"
     CDS             complement(17790..18026)
                     /gene="lpp"
                     /locus_tag="OO230_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5692444.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18337..19749)
                     /gene="pykF"
                     /locus_tag="OO230_002750"
     CDS             complement(18337..19749)
                     /gene="pykF"
                     /locus_tag="OO230_002750"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5692445.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5311 a         4860 c         5176 g         4932 t
ORIGIN      
        1 tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc ttatgcctga
       61 tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg cgcagaggct
      121 ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag acgagaaatt
      181 acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg ctcctgcatc
      241 tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat cagcgcaatc
      301 ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat ttcttcggct
      361 tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg cgcgtgggcg
      421 taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc gatcatatca
      481 accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt ggaaatcgcc
      541 tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc tggcatggtt
      601 tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc ggcaaagtgg
      661 tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc ttcggatttc
      721 acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc cgcaccaatg
      781 acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt
      841 caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt gccagtccat
      901 aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat cgttttgcaa
      961 ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac gcattcagtg
     1021 accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt ctcaaggggt
     1081 ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc ttcagcaaag
     1141 gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc gttaacccgc
     1201 tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag tttaatttct
     1261 tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc ggcccctaac
     1321 agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg aatatggccc
     1381 gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt gttgatggca
     1441 cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag ttgtttgttc
     1501 ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat tgctccggga
     1561 aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc tttttctaag
     1621 gctttattct gcatttcagg cgataaactg tggcggatag gataagccat caatccaatc
     1681 aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc ttaattgtgt
     1741 ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga ataataaaaa
     1801 cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc gcgaaagtaa
     1861 aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta atcagcggaa
     1921 taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg gctttgcttt
     1981 tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca gctgctgaga
     2041 aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg tagataatgg
     2101 cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc cggaccattt
     2161 ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg taataagaga
     2221 tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat ttgggcatcc
     2281 acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc gatgaaacac
     2341 cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct acgctggcgt
     2401 ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt acaaacagaa
     2461 tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata tagctcacca
     2521 gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg accgccgaac
     2581 cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc agcgctgagt
     2641 tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg caagcgggaa
     2701 taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc cgacgaccaa
     2761 acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa ccgataccgg
     2821 aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgacaga gatgtcatat
     2881 tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa atacaaagaa
     2941 cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt gttctccgac
     3001 ggtttacata attactatac tgaagatgat tttccctggg tacatggcat aaagtttatg
     3061 tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt agttagcgca
     3121 gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc attacccacc
     3181 cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc gtaagccgat
     3241 aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg caatacttct
     3301 ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc ccgtactgta
     3361 atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta acgtcaggcc
     3421 aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata ttatcaccac
     3481 ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa aggtgtacag
     3541 catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa taaatacaca
     3601 aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt atgacatgcc
     3661 tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg ttgccattga
     3721 gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat gttccgtaga
     3781 gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga acgtacaacg
     3841 gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg accaaccgaa
     3901 ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt gtccgctgga
     3961 aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag cttccatcaa
     4021 actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa agccaaaaac
     4081 ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca tatagcagca
     4141 catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag
     4201 caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac ccgcggcatt
     4261 agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga cgcccatacc
     4321 atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat ttttcatagg
     4381 ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta
     4441 ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct ccagatcgtg
     4501 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
     4561 gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
     4621 gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac gcatagccaa
     4681 cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact gacgccagct
     4741 ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
     4801 atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
     4861 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
     4921 caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc
     4981 ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact caggggattt
     5041 ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg catacggact
     5101 ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt gcgacaggta
     5161 ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa tatcaattag
     5221 cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt cggtttctcg
     5281 atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg gacttcttcc
     5341 acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat aaacagaccg
     5401 atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat cagcggtaaa
     5461 tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc aaccacaccg
     5521 ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag ccagataatc
     5581 gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat cattaacacc
     5641 gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc aagcactgcc
     5701 tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg tgccgccagc
     5761 agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg aatgccttgt
     5821 gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag cgcacaatgc
     5881 accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata
     5941 taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt gtgccagcct
     6001 gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc gggtaacgtc
     6061 atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat actgttaacc
     6121 agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac cagaacggcg
     6181 agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca ggtggcgata
     6241 accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca cagacatgcc
     6301 acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc cctgggctga
     6361 cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg
     6421 aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa caggccgatt
     6481 ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact caggcaacac
     6541 gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt cgttcacccc
     6601 agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct gcaactcgaa
     6661 atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct gaaagaatag
     6721 tgtatcgacc tacatcacag accgcaggaa agggtcagta taatgattcc acagatttcc
     6781 ctggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga gcaacaaggt
     6841 tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac cgacaacagt
     6901 atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg
     6961 atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc ccgcggcggc
     7021 ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat gtcccgccat
     7081 atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga ggccggggtg
     7141 ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc gccggaactt
     7201 tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc cggtcaggga
     7261 tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt gttgttgggg
     7321 ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct gggtaaatcc
     7381 aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca gcaacgccag
     7441 ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga tctgcgtcat
     7501 gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg
     7561 acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa agtgcgccgt
     7621 ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc gtttatggtt
     7681 gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct ggcgcgggaa
     7741 gatattgtct ggcattccgt cagcgagttg attaccgata tgcctgacaa agagatgctc
     7801 ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga gcgagttaat
     7861 gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt gattggctgg
     7921 caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa aaaagccgtt
     7981 ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga ggatacctgc
     8041 gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct cgacagccac
     8101 ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt ccgtccggca
     8161 ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga tgacgtggtg
     8221 gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg ttttcgcgct
     8281 gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg
     8341 gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga aggtctcgat
     8401 gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca gatccccatt
     8461 gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac
     8521 tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg gattcattca
     8581 ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga tcgcggcgtt
     8641 gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg gacgttaatt
     8701 gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc acacgaagtc
     8761 aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg ccccatcaaa
     8821 attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg ttatttacgc
     8881 ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat ggcacgcgcg
     8941 ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc ggaaaaacat
     9001 atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca aatggtgggg
     9061 catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga gcagaaagcg
     9121 cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca agtggtggcg
     9181 gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc attttcgcca
     9241 aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac ggcgaaaaag
     9301 acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg cgtcgatccg
     9361 gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga acgtggcgag
     9421 tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca tccagtggct
     9481 acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac cgctttgccg
     9541 ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc
     9601 agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa ccatgaaaat
     9661 tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc gcgtaaccgc
     9721 tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg cacgggggta
     9781 cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg aaaatcaccc
     9841 tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat attcgctttg
     9901 aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg acaaagcagc
     9961 ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc ggttccgttg
    10021 ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc aatgctaatc
    10081 acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg catctcggtt
    10141 cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg tgctgttcgt
    10201 cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt
    10261 ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct aaaagcgagg
    10321 atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta cgccgtgacc
    10381 agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa cttgaatttg
    10441 ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt gcctggcgtc
    10501 tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct ggattaacct
    10561 ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat tgttcaaaaa
    10621 taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt attcttatcg
    10681 ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg ggtagaaacc
    10741 cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg cactttttta
    10801 cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca gccttaatgc
    10861 tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt gcctgttaac
    10921 ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta cggtaaagcc
    10981 cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta tacgctgaaa
    11041 gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc acaggatttc
    11101 gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga gctggtggca
    11161 aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg cgcgggcttt
    11221 ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt tgaacacgac
    11281 ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac ggaagtcgat
    11341 ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc ccagaatgaa
    11401 tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat actgaagcaa
    11461 ctgacgatgt caaaacctgg accggcggcc cgctgaacta taaagaagga ttcttcaccc
    11521 agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc gcaatttcgg
    11581 cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat cgcgcatggc
    11641 tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat tatcaggatt
    11701 acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg tctgaacctg
    11761 gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag gcggcgtttg
    11821 agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc attttcgact
    11881 ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt attttctgtt
    11941 cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc ggcaccgtgg
    12001 tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct gacggtacgt
    12061 ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat tttcgcatta
    12121 acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa gacagctacg
    12181 tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg cacgcggcag
    12241 tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta caaaactggt
    12301 tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt gctttgtgcg
    12361 aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg attacgtgga
    12421 aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac tcagtggcgc
    12481 tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc ggtaaaaaca
    12541 ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac agttatcgcg
    12601 gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag tgcgactcaa
    12661 tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt cgcaataaca
    12721 gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa ctgttttact
    12781 gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac ggtttctgca
    12841 aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa ctcctcgcca
    12901 tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa gatttacacg
    12961 tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt cccggcgaag
    13021 ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca acgcttgccg
    13081 ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa gatttgcttg
    13141 cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag tatccggtgg
    13201 agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca gtgcgcagct
    13261 atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa gagaaaatcg
    13321 ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt ttttccggcg
    13381 gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag ttatgcattc
    13441 ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc gatggcgtga
    13501 actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa cgcattctcg
    13561 actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg aaatccggcg
    13621 atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc gaacagcagt
    13681 aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg ctcaccgcaa
    13741 gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa acatgaaaac
    13801 tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat tgcgggagat
    13861 atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg gctggtgttt
    13921 gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg atatgaagtg
    13981 agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt gtttctgcat
    14041 ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg tcaacggccg
    14101 gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga ggtgaacact
    14161 gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt gatcgaacat
    14221 tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat caatgtcgca
    14281 gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag tcaccacttt
    14341 gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag tttcctgctg
    14401 ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa cagcacgctg
    14461 cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg tacctggctg
    14521 gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt cagcgacaaa
    14581 ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa aacggatggt
    14641 cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac aaaaccgcag
    14701 ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg gcgtattgat
    14761 gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc ccagcagatg
    14821 atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg gcttaaacag
    14881 caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact ttttccgtcg
    14941 acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg ccgctggctt
    15001 atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc gaggccgagt
    15061 tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc gcccaggcga
    15121 ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc cgttcggcgg
    15181 aagagctggt gtttgtccgc ggcacgacgg aagggatcaa tctggtcgcc aatagctggg
    15241 gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag caccacgcta
    15301 acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt gtgatccctc
    15361 tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat gagaaaactc
    15421 gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca ctggcggaaa
    15481 tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct caggcggtga
    15541 tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc tccgggcata
    15601 aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg ttgcaggaga
    15661 tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt gaaggcacta
    15721 cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg ggcatcattg
    15781 gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata gccgagtatg
    15841 aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat ctcactctct
    15901 atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac cacgcctatg
    15961 atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat cactgcgcta
    16021 tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg gccatgtata
    16081 acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac cgtctgctgg
    16141 gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg cgtaattttt
    16201 tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc cagcgtctgc
    16261 cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc cagagtcagg
    16321 tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc gacagcgatg
    16381 cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag atgacgccgc
    16441 aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc acccaacatc
    16501 tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc gccaaagccg
    16561 ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt gccggatggt
    16621 caatgtttac actgaccggc acaggagtgt tttggtttca ggtgaacata agcaccatcc
    16681 gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg cgcgtctttg
    16741 cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc ggttgattat
    16801 ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac ggtgcaagaa
    16861 ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat gttgatcctt
    16921 gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac tattccttca
    16981 caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc agagctgcgc
    17041 ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg tattggattg
    17101 caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc taacccaacc
    17161 tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa attaccgcca
    17221 gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct cgcgcatggt
    17281 aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt gcgcgtcagt
    17341 tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag cgtgcggacg
    17401 ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc taacgggatg
    17461 cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt tcagacaatg
    17521 ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt agatcagaag
    17581 ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag cagtggagca
    17641 actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga accagagcaa
    17701 gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat ggcgcacaat
    17761 gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc catgttgtcc
    17821 agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga acgcattgcg
    17881 ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc agaagacagc
    17941 tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag gattaccgcg
    18001 cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt aatctccatg
    18061 tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg atgggaaggc
    18121 acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga taaaaaaatt
    18181 gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt atatagtgaa
    18241 atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc gcccatcagg
    18301 gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa cagatgcggt
    18361 gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt cacctttgtg
    18421 tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat cagtagaagt
    18481 gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat gagccgtttt
    18541 ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta ctgcgcgagc
    18601 agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt cagcagtttc
    18661 aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat tgttgaactc
    18721 gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc
    18781 ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt cagtaccgtc
    18841 gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat
    18901 ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac atttttcgat
    18961 catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca ggtcgccacg
    19021 cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga ggccttcctg
    19081 gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg
    19141 gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt ctacgccttt
    19201 ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca gagcaatgga
    19261 aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca gcactttaca
    19321 gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat cgtcaaccag
    19381 tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg caaccatttc
    19441 gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag ctttcagaga
    19501 aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg tatcaagcag
    19561 gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa tgcgctgacc
    19621 gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc cagcgtccag
    19681 cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa caattttggt
    19741 ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt gctttctggg
    19801 cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct ctaaggatag
    19861 gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgtc
    19921 tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag agtcaattgg
    19981 cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat
    20041 acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa aaggttacgc
    20101 aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag gataagtgaa
    20161 agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat aatttatcgc
    20221 gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat atgctgata
//
LOCUS       DAJTPG010000075        20219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTPG010000075 DAJTPG010000000
VERSION     DAJTPG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO230_002751"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO230_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5692446.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO230_002752"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO230_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5692447.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO230_002753"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO230_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="OO230_002754"
     CDS             1631..2341
                     /locus_tag="OO230_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5692448.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO230_002755"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO230_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5692449.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="OO230_002756"
     CDS             complement(3469..4119)
                     /locus_tag="OO230_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5692450.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="OO230_002757"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="OO230_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5692451.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="OO230_002758"
     CDS             complement(6584..7024)
                     /locus_tag="OO230_002758"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5692452.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="OO230_002759"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="OO230_002759"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5692453.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7742..8440
                     /locus_tag="OO230_002760"
     CDS             7742..8440
                     /locus_tag="OO230_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5692454.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9877
                     /locus_tag="OO230_002761"
     CDS             8669..9877
                     /locus_tag="OO230_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692455.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10201..11892
                     /gene="eptB"
                     /locus_tag="OO230_002762"
     CDS             10201..11892
                     /gene="eptB"
                     /locus_tag="OO230_002762"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5692456.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11984..12060
                     /locus_tag="OO230_002763"
     tRNA            11984..12060
                     /locus_tag="OO230_002763"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12018..12020,aa:Pro,seq:cgg)
     gene            12804..14411
                     /gene="dppA"
                     /locus_tag="OO230_002764"
     CDS             12804..14411
                     /gene="dppA"
                     /locus_tag="OO230_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5692457.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14719..15738
                     /gene="dppB"
                     /locus_tag="OO230_002765"
     CDS             14719..15738
                     /gene="dppB"
                     /locus_tag="OO230_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5692458.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15748..16650
                     /gene="dppC"
                     /locus_tag="OO230_002766"
     CDS             15748..16650
                     /gene="dppC"
                     /locus_tag="OO230_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5692459.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16661..17644
                     /gene="dppD"
                     /locus_tag="OO230_002767"
     CDS             16661..17644
                     /gene="dppD"
                     /locus_tag="OO230_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5692460.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17641..18645
                     /gene="dppF"
                     /locus_tag="OO230_002768"
     CDS             17641..18645
                     /gene="dppF"
                     /locus_tag="OO230_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5692461.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18675..19946)
                     /locus_tag="OO230_002769"
     CDS             complement(18675..19946)
                     /locus_tag="OO230_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5692462.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4844 a         5007 c         5306 g         5062 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4741 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4921 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     5461 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     5521 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5881 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     6061 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     6121 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     6181 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     6361 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6781 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6901 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     7021 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     7141 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     7381 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     8401 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     8461 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgttatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8761 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8881 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8941 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9601 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9781 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9841 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9901 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9961 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
    10021 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
    10081 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
    10141 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
    10201 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
    10261 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
    10321 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
    10381 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
    10441 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
    10501 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10561 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10621 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10681 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10741 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10801 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10861 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10921 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10981 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    11041 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    11101 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    11161 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    11221 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    11281 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    11341 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    11401 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    11461 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    11521 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11581 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11641 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11701 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11761 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11821 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11881 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11941 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    12001 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    12061 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    12121 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    12181 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    12241 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    12301 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    12361 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    12421 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    12481 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12541 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12601 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12661 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12721 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12781 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12841 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12901 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12961 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    13021 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    13081 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    13141 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    13201 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    13261 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    13321 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    13381 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    13441 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    13501 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13561 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13621 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13681 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13741 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13801 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13861 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13921 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13981 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    14041 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    14101 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    14161 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    14221 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    14281 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    14341 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    14401 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    14461 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    14521 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14581 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14641 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14701 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14761 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14821 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14881 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14941 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    15001 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    15061 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    15121 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    15181 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    15241 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    15301 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    15361 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    15421 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    15481 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15541 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15601 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15661 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15721 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15781 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15841 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15901 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15961 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    16021 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    16081 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat
    16141 aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    16201 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt
    16261 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    16321 tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc
    16381 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    16441 ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg
    16501 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    16561 cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    16621 gacgcgctcg atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga
    16681 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    16741 ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc
    16801 ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa
    16861 actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    16921 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    16981 cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg
    17041 ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct
    17101 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    17161 gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac
    17221 cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct
    17281 ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat
    17341 gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca
    17401 cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct
    17461 ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa
    17521 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    17581 cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact
    17641 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    17701 cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc
    17761 gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt
    17821 aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat
    17881 tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    17941 atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa
    18001 attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa
    18061 gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac
    18121 atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg
    18181 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    18241 ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac
    18301 gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc
    18361 gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg
    18421 ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc
    18481 ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt
    18541 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg
    18601 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    18661 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    18721 taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag
    18781 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    18841 aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    18901 cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac
    18961 agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg
    19021 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc
    19081 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    19141 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata
    19201 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt
    19261 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa
    19321 aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata
    19381 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    19441 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    19501 atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    19561 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    19621 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    19681 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    19741 aaataaatgt cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat
    19801 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    19861 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg
    19921 tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    19981 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    20041 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    20101 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa
    20161 attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagca
//
LOCUS       DAJTPG010000076        19703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTPG010000076 DAJTPG010000000
VERSION     DAJTPG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO230_002770"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO230_002770"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5692463.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO230_002771"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO230_002771"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5692464.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO230_002772"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO230_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5692465.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO230_002773"
     CDS             6038..6658
                     /locus_tag="OO230_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5692466.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO230_002774"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO230_002774"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5692467.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO230_002775"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO230_002775"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5692468.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO230_002776"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO230_002776"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5692469.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO230_002777"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO230_002777"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5692470.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO230_002778"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO230_002778"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO230_002779"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO230_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO230_002780"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO230_002780"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5692471.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO230_002781"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO230_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5692472.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO230_002782"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO230_002782"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5692473.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO230_002783"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO230_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5692474.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO230_002784"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO230_002784"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5692475.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="OO230_002785"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="OO230_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5692476.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4682 a         5508 c         5034 g         4479 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtc cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttttc ggt
//
LOCUS       DAJTPG010000077        19287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTPG010000077 DAJTPG010000000
VERSION     DAJTPG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            44..1540
                     /gene="ravA"
                     /locus_tag="OO230_002786"
     CDS             44..1540
                     /gene="ravA"
                     /locus_tag="OO230_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5692477.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1534..2985
                     /gene="viaA"
                     /locus_tag="OO230_002787"
     CDS             1534..2985
                     /gene="viaA"
                     /locus_tag="OO230_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5692478.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2990..3982)
                     /gene="asnA"
                     /locus_tag="OO230_002788"
     CDS             complement(2990..3982)
                     /gene="asnA"
                     /locus_tag="OO230_002788"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5692479.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4134..4592
                     /gene="asnC"
                     /locus_tag="OO230_002789"
     CDS             4134..4592
                     /gene="asnC"
                     /locus_tag="OO230_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5692480.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4682..5125
                     /gene="mioC"
                     /locus_tag="OO230_002790"
     CDS             4682..5125
                     /gene="mioC"
                     /locus_tag="OO230_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5692481.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5504..7393
                     /gene="mnmG"
                     /locus_tag="OO230_002791"
     CDS             5504..7393
                     /gene="mnmG"
                     /locus_tag="OO230_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5692482.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7457..8080
                     /gene="rsmG"
                     /locus_tag="OO230_002792"
     CDS             7457..8080
                     /gene="rsmG"
                     /locus_tag="OO230_002792"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5692483.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8697..9077
                     /gene="atpI"
                     /locus_tag="OO230_002793"
     CDS             8697..9077
                     /gene="atpI"
                     /locus_tag="OO230_002793"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5692484.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9086..9901
                     /gene="atpB"
                     /locus_tag="OO230_002794"
     CDS             9086..9901
                     /gene="atpB"
                     /locus_tag="OO230_002794"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5692485.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9948..10187
                     /gene="atpE"
                     /locus_tag="OO230_002795"
     CDS             9948..10187
                     /gene="atpE"
                     /locus_tag="OO230_002795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5692486.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10249..10719
                     /gene="atpF"
                     /locus_tag="OO230_002796"
     CDS             10249..10719
                     /gene="atpF"
                     /locus_tag="OO230_002796"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5692487.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10734..11267
                     /gene="atpH"
                     /locus_tag="OO230_002797"
     CDS             10734..11267
                     /gene="atpH"
                     /locus_tag="OO230_002797"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5692488.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11280..12821
                     /gene="atpA"
                     /locus_tag="OO230_002798"
     CDS             11280..12821
                     /gene="atpA"
                     /locus_tag="OO230_002798"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5692489.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12872..13735
                     /gene="atpG"
                     /locus_tag="OO230_002799"
     CDS             12872..13735
                     /gene="atpG"
                     /locus_tag="OO230_002799"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5692490.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13762..15144
                     /gene="atpD"
                     /locus_tag="OO230_002800"
     CDS             13762..15144
                     /gene="atpD"
                     /locus_tag="OO230_002800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5692491.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15165..15584
                     /gene="atpC"
                     /locus_tag="OO230_002801"
     CDS             15165..15584
                     /gene="atpC"
                     /locus_tag="OO230_002801"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5692492.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15935..17305
                     /gene="glmU"
                     /locus_tag="OO230_002802"
     CDS             15935..17305
                     /gene="glmU"
                     /locus_tag="OO230_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5692493.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17467..>19287
                     /gene="glmS"
                     /locus_tag="OO230_002803"
     CDS             17467..>19287
                     /gene="glmS"
                     /locus_tag="OO230_002803"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5692494.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVT"
BASE COUNT         4741 a         4835 c         5103 g         4608 t
ORIGIN      
        1 cgcaatttac gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt
       61 attagcggaa agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag
      121 ccacgccatc cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg
      181 cccgccaggt attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc
      241 ccgcgcgttt gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct
      301 ttctattcag gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc
      361 ggaagcagaa atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac
      421 cttgctcacc gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc
      481 gatgcgtcta ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc
      541 gttatatgac cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt
      601 ccgctccatg ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca
      661 ggtcacagat gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga
      721 tcatgtattt gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc
      781 ttatgtctcg gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt
      841 tagcggtcgc agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta
      901 tgacgcgcaa agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc
      961 ctggcaacag caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact
     1021 ccagcagcaa caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag
     1081 ccgtcgtcag cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct
     1141 gcaaaaaccg ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc
     1201 gctggaacag tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt
     1261 tgcccagaaa ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag
     1321 tttacaaggc agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat
     1381 aaaacaacaa ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga
     1441 acagcaaaaa tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca
     1501 ggatgtcggt gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg
     1561 atgctggccg tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca
     1621 ccgcagctgg cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat
     1681 gttccccgct ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa
     1741 ctcaccgaag aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt
     1801 gtgcagctac cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa
     1861 gcccggcagt tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc
     1921 cagcgttggc gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa
     1981 gaagaacgcg aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt
     2041 gaaccgattc tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt
     2101 caacttaaac gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag
     2161 ccggaactga aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg
     2221 cgcaacgatg cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct
     2281 gagcaggttg atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg
     2341 gcgacactag ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag
     2401 ttgctcacct atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg
     2461 gtacataaag attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc
     2521 ggctcaatgg gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc
     2581 attgctctcg ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt
     2641 tatgagcttt caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt
     2701 cgtggcggca ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg
     2761 gaatggtttg atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac
     2821 gacgtgacga gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg
     2881 gcgatgtcgg cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt
     2941 gataccggga tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag
     3001 aagggacgct ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg
     3061 gcagttgcag cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg
     3121 gcatttcacc gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg
     3181 tcagcgccag ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct
     3241 caaacgcatc ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc
     3301 ccagctctga cagggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac
     3361 catcactcaa cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg
     3421 cccgctcacg ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta
     3481 cgaagtgaat ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt
     3541 cggttgcttt aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac
     3601 gctcaccgtc gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg
     3661 gagaaagacg gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg
     3721 cgctgaagtc gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt
     3781 cgaactgggc atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca
     3841 agttatcctg cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc
     3901 ccagacgttc ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt
     3961 tggcaatgta agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca
     4021 acaaaaaccg acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca
     4081 ttaaaaaaga cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa
     4141 attatctgat cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca
     4201 ccgcttacgc cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag
     4261 tagagaaaat gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc
     4321 agctcggtta tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc
     4381 cttccgcgct ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg
     4441 gccactacag catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac
     4501 ttatcaacaa gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc
     4561 aaaacccgat catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca
     4621 caggtagatc ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt
     4681 tatggcagat atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga
     4741 acacctggct gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct
     4801 gttggaagat ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg
     4861 agatattccg gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct
     4921 ttctgcggtc cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg
     4981 ggctatcgat aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac
     5041 actgaagatc aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg
     5101 atcgtggatt aatttactca aataagtata cagatcgtgc gatctactgt ggataactct
     5161 gtcaggaagc ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag
     5221 ttgtgtataa cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac
     5281 agttaatgat cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc
     5341 agtgcgatcc taataagaga tcacaataga acagatctct aaataaatag atcttctttt
     5401 taatacccag gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg
     5461 ggcttcaatc cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc
     5521 ttttgacgtc atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc
     5581 gcgtatgggt caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag
     5641 ctgcaacccg gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact
     5701 cggcggtctg atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc
     5761 aagcaaagga ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca
     5821 ggcggtacgt acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga
     5881 agatcttatt gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt
     5941 ccgtgccaaa gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg
     6001 tctggataat tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg
     6061 tttgcgtgaa ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga
     6121 tgctcgaacc atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt
     6181 attctcgttt atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca
     6241 taccaacgag aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc
     6301 aggggtgatc gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg
     6361 cttcgcagac agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga
     6421 aatttatccg aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg
     6481 ctctatgcag gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga
     6541 cttcttcgat cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt
     6601 ctttgctggt cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct
     6661 ggccggtctt aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc
     6721 tcaggcgtat ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta
     6781 tcgtatgttt acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct
     6841 gcgtttgact gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt
     6901 taacgagaaa cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac
     6961 cccgtcggcg gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga
     7021 agccagtggt gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct
     7081 gacgccgttt gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt
     7141 taaatacgaa ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga
     7201 gaacaccctg ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt
     7261 gatcgccaaa cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg
     7321 cgtcacgcct gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg
     7381 tcgtagcgca taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca
     7441 agaacaggta atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc
     7501 gcttactgat caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa
     7561 caaagcgtac aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct
     7621 cgatagcatt gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg
     7681 accaggactg ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt
     7741 ggatagcctt ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga
     7801 gaatattgaa ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg
     7861 cgtaattagc cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc
     7921 tggtgagcaa ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt
     7981 gttgcccgaa gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg
     8041 cgaacgtcat ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata
     8101 aaaaattgac cggttagact gttaacaaca accaggtttt ctactgatat aactggttac
     8161 atttaacgcc acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca
     8221 gaaaataagt cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt
     8281 ggctgttaaa acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta
     8341 ttaaaacagt atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca
     8401 agtaaataaa tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca
     8461 ccagtgttta cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc
     8521 aattgtatgc actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg
     8581 aaatcacggg ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa
     8641 agaaagtttt atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt
     8701 ctgtgtcgct cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg
     8761 tgatagcaag tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa
     8821 gcgggggcct ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc
     8881 aggcgcatac accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca
     8941 aagttctggc gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct
     9001 tgccgctgat cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa
     9061 ttaacaacaa agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat
     9121 aggacaccac ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca
     9181 aaacccccca gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct
     9241 gggtctgttg ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc
     9301 aggtaagttt cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga
     9361 catgtaccat ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt
     9421 attcctgatg aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca
     9481 tgtactgggt ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc
     9541 tatggcactg ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg
     9601 cggcttcacg aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa
     9661 cttaatcctt gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt
     9721 cggtaacatg tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg
     9781 gtcacagtgg atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca
     9841 agccttcatc ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta
     9901 atttaccaac actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga
     9961 atatggatct gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg
    10021 cgatcggtat cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc
    10081 tgattcctct gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga
    10141 tgatcgctgt aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt
    10201 tatttaaaga gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac
    10261 gcaacaatcc tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac
    10321 gtatggccgc cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt
    10381 gcttccgcag aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag
    10441 ctgaaaaaag cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg
    10501 cagattctgg acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag
    10561 gcgcaggcag aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt
    10621 gctatcctgg ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct
    10681 aacagcgaca tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg
    10741 aatttattac ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc
    10801 aaagtgtaga acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac
    10861 aaatggcaga gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg
    10921 cagtttgtgg tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa
    10981 atggtcgtct taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga
    11041 gtgaggctac cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg
    11101 cgaaaatttc tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa
    11161 tcgataagtc tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca
    11221 gcgtacgcgg tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca
    11281 tgcaactgaa ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg
    11341 ttgtgagtga agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca
    11401 ttcacggcct ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg
    11461 ctatcgcact gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg
    11521 accttgccga aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc
    11581 gtggcctgct gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc
    11641 tggatcacga cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt
    11701 ccgtagatca gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc
    11761 gtggtcagcg tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg
    11821 atgccatcat caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga
    11881 aagcgtccac catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca
    11941 ccatcgttgt ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg
    12001 ccggttgcgc aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg
    12061 atgacctgtc taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc
    12121 caggacgtga agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg
    12181 ctgcacgtgt taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa
    12241 ccggttctct gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg
    12301 ttccgaccaa cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca
    12361 acgccggtat tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag
    12421 cacagaccaa gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc
    12481 gtgaactggc agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc
    12541 ttgaccacgg tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg
    12601 ttgcgcagca gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac
    12661 tgtcgaaaat tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc
    12721 cgttgatgca agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga
    12781 aaggcatcct cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta
    12841 gggcaggccg caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa
    12901 gatcgcaagc gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc
    12961 caaaatgcgt aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg
    13021 caaagtgatt ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga
    13081 ccgcgacgtt aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg
    13141 tttgaacatt aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg
    13201 cgttcaatgc gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg
    13261 cggcaatgtt gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat
    13321 cggtccggta aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat
    13381 tgtcagcaac aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc
    13441 gttaccggca tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc
    13501 cgatccgaag gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca
    13561 gggcgtggtt gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc
    13621 gaccgacaat ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca
    13681 ggccagcatt actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg
    13741 ttatttcgta gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta
    13801 gttgacgtcg aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa
    13861 aatggtaatg agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt
    13921 accatcgcaa tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa
    13981 cacccgatcg aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt
    14041 gaaccggtcg acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca
    14101 gcaccttcct acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt
    14161 atcgacctga tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt
    14221 gtaggtaaaa ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt
    14281 tactctgtgt ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa
    14341 atgaccgact ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg
    14401 ccgggaaacc gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac
    14461 gaaggtcgtg acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg
    14521 gaagtatccg cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg
    14581 gaagagatgg gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc
    14641 gtacaggcgg tatatgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt
    14701 gcgcaccttg acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg
    14761 gccgttgacc cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa
    14821 cactacgaca ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac
    14881 atcatcgcca tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt
    14941 gctcgtaaga tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt
    15001 tctccgggta aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa
    15061 ggcgaatacg atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct
    15121 gtggaaaaag ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc
    15181 acctggacgt cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg
    15241 taacgggtag cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca
    15301 ttaagcctgg tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt
    15361 ctggcggcat tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc
    15421 gcggccagga tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca
    15481 ttagcagctc tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga
    15541 tcgcgcagct gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc
    15601 acaaaagcca gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg
    15661 ttcacataga tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa
    15721 taagagcaaa aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat
    15781 aaaaggcgtg acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa
    15841 caatttatcc tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt
    15901 ttaaattcgt tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt
    15961 gatccttgcc gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac
    16021 ccttgccggg aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc
    16081 gcacgttcac ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga
    16141 caaccttaac tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc
    16201 cgcacctttc tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat
    16261 ctctgtcgaa acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct
    16321 gacggtgaaa ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt
    16381 taccggcatt gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa
    16441 caccggcatt ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa
    16501 caataatgct cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg
    16561 gcgtgaaatc gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg
    16621 cctgcaactc tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt
    16681 agcaggcgtt atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg
    16741 gcgcgatgtt gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg
    16801 cgtgaaaatc ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat
    16861 cagtccgtat accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt
    16921 tgcccgtctg cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga
    16981 gatgaaaaaa gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga
    17041 tgcggaaatt ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg
    17101 tgcgaataaa tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct
    17161 ggtggccccg gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg
    17221 taatgtcggc gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg
    17281 gcgtcgtccg gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga
    17341 gataacataa tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg
    17401 ctcggggcgc ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca
    17461 aaaactatgt gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt
    17521 gaaggtttac gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat
    17581 gcggaaggtc atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca
    17641 gcggaagaac atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac
    17701 ggtgaacctt cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat
    17761 aatggcatca tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc
    17821 ttcgtttctg aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa
    17881 ggcgggactc tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt
    17941 acagtgatca tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg
    18001 ctggtgattg gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg
    18061 ccggtgaccc gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt
    18121 tcggtaaaca tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat
    18181 ctgcaatatg acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac
    18241 gaacagccga acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat
    18301 ttaagcgagc tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc
    18361 ctcgcctgtg gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg
    18421 gcaggtattc cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg
    18481 cgtcgtaaca gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg
    18541 ggcctgcgtc tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg
    18601 ggttcttctc tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc
    18661 ggcgtggcgt ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg
    18721 aagctgtctc gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg
    18781 caggcgctgc cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg
    18841 gcagaagatt tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca
    18901 atcgcgctgg aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac
    18961 gctgcaggcg agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc
    19021 gtcgttgcac cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc
    19081 gcgcgtggcg gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat
    19141 aacatgcaca tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc
    19201 gttccgctgc agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag
    19261 ccgcgtaacc tggcaaaatc ggttacg
//
LOCUS       DAJTPG010000078        18473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTPG010000078 DAJTPG010000000
VERSION     DAJTPG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(139..3654)
                     /locus_tag="OO230_002804"
     CDS             complement(139..3654)
                     /locus_tag="OO230_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5692495.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO230_002805"
     CDS             complement(4107..5369)
                     /locus_tag="OO230_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5692496.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO230_002806"
     tRNA            complement(5567..5642)
                     /locus_tag="OO230_002806"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO230_002807"
     CDS             complement(5748..6455)
                     /locus_tag="OO230_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5692497.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO230_002808"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO230_002808"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5692498.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO230_002809"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO230_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5692499.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO230_002810"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO230_002810"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5692500.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO230_002811"
     CDS             complement(11639..11914)
                     /locus_tag="OO230_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5692501.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO230_002812"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO230_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5692502.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO230_002813"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO230_002813"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5692503.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO230_002814"
     CDS             13874..14200
                     /locus_tag="OO230_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5692504.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO230_002815"
     CDS             14384..15103
                     /locus_tag="OO230_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5692505.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO230_002816"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO230_002816"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5692506.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO230_002817"
     CDS             16442..17404
                     /locus_tag="OO230_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5692507.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18473)
                     /locus_tag="OO230_002818"
     CDS             complement(17505..>18473)
                     /locus_tag="OO230_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR5692508.1"
                     /translation="ISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLI
                     PPSNVLIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFA
                     QACKAFLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLP
                     KLILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVL
                     LIVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVG
                     CSISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4782 a         4502 c         4570 g         4619 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aat
//
LOCUS       DAJTPG010000079        18350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTPG010000079 DAJTPG010000000
VERSION     DAJTPG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..1228
                     /locus_tag="OO230_002819"
                     /pseudo
     CDS             1..1228
                     /locus_tag="OO230_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1378..1809
                     /gene="ndk"
                     /locus_tag="OO230_002820"
     CDS             1378..1809
                     /gene="ndk"
                     /locus_tag="OO230_002820"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5692509.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            1959..3113
                     /gene="trmG/rlmN"
                     /locus_tag="OO230_002821"
     CDS             1959..3113
                     /gene="trmG/rlmN"
                     /locus_tag="OO230_002821"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5692510.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3398..4411
                     /gene="rodZ"
                     /locus_tag="OO230_002822"
     CDS             3398..4411
                     /gene="rodZ"
                     /locus_tag="OO230_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5692511.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4438..5565
                     /gene="ispG"
                     /locus_tag="OO230_002823"
                     /gene_synonym="gcpE"
     CDS             4438..5565
                     /gene="ispG"
                     /locus_tag="OO230_002823"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5692512.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5668..6942
                     /gene="hisS"
                     /locus_tag="OO230_002824"
     CDS             5668..6942
                     /gene="hisS"
                     /locus_tag="OO230_002824"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5692513.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            6960..7580
                     /gene="yfgM"
                     /locus_tag="OO230_002825"
     CDS             6960..7580
                     /gene="yfgM"
                     /locus_tag="OO230_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5692514.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7591..8769
                     /gene="bamB"
                     /locus_tag="OO230_002826"
     CDS             7591..8769
                     /gene="bamB"
                     /locus_tag="OO230_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5692515.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            8887..10359
                     /gene="der"
                     /locus_tag="OO230_002827"
     CDS             8887..10359
                     /gene="der"
                     /locus_tag="OO230_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5692516.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10429..10644
                     /locus_tag="OO230_002828"
     CDS             10429..10644
                     /locus_tag="OO230_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5692517.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10641..12011)
                     /gene="xseA"
                     /locus_tag="OO230_002829"
     CDS             complement(10641..12011)
                     /gene="xseA"
                     /locus_tag="OO230_002829"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5692518.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12173..13639
                     /gene="guaB"
                     /locus_tag="OO230_002830"
     CDS             12173..13639
                     /gene="guaB"
                     /locus_tag="OO230_002830"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5692519.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13708..15285
                     /gene="guaA"
                     /locus_tag="OO230_002831"
     CDS             13708..15285
                     /gene="guaA"
                     /locus_tag="OO230_002831"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5692520.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15380..15919)
                     /locus_tag="OO230_002832"
     CDS             complement(15380..15919)
                     /locus_tag="OO230_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5692521.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15935..16453)
                     /locus_tag="OO230_002833"
     CDS             complement(15935..16453)
                     /locus_tag="OO230_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5692522.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(16764..16955)
                     /locus_tag="OO230_002834"
     CDS             complement(16764..16955)
                     /locus_tag="OO230_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5692523.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            16973..17125
                     /locus_tag="OO230_002835"
     CDS             16973..17125
                     /locus_tag="OO230_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692524.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17307..>18350
                     /gene="pdeF"
                     /locus_tag="OO230_002836"
     CDS             17307..>18350
                     /gene="pdeF"
                     /locus_tag="OO230_002836"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5692525.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRAL"
BASE COUNT         4341 a         4456 c         4966 g         4587 t
ORIGIN      
        1 cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg ggttaatcca
       61 ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat ttccggtggc
      121 agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac atttggtggc
      181 aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa gcgtgaaatt
      241 ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat cggtgcggca
      301 agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc ggcaatgctg
      361 gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga gcgtgccgaa
      421 gccgcgcgtc gccccactgg catggaaaac gggcaccagc tatggctatc gtgacgcctg
      481 ggcgattggg gttaacgctc gctatgtcat tgggatctgg actggcagac ctgacggcac
      541 gcccgttgtt ggtcagtttg gctttgccag tgccgtacca ttgttgaatc aggtcaataa
      601 tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg cgcccgaact cagtcactcg
      661 tggcgttatc tgctggccgg gcggacagtc tttgccggaa ggtgacggta actgtcgccg
      721 ccgcctggca acctggctgc tggatggcag ccagccgcca acgctgttac tgtcagagca
      781 ggaagggatt aatggcattc gcttccccat ctggctggat gacaaagggc agcgcgtcgc
      841 cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc tggccgctac cgctggaacc
      901 ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca ccggcctcga caatttgccc
      961 gccatacggt cacgatgccc agctccctct gcagctgaca ggcgttcgcg atggcgcgat
     1021 tattaaacgt ttaccgggcg cagcggaagc gactttgccg ttgcaatcaa gtggaggggc
     1081 aggtgaacgc tggtggtttc tgaatggcga accgttaact gaacgcgggc gcaacgtgac
     1141 cctgcatttg acggataaag gtgattatca attgctggtg atggatgatg tggggcaaat
     1201 cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc ctgattgttg ctaaaactca
     1261 ttttatttta aaaaaaatgt taccttcatc aatagtcaac ggccctgttg ctcattataa
     1321 tccgcgccat ctcgtacgct ggtacagaca acaacagaac aatttacaga ggtaaaaatg
     1381 gctattgaac gtactttttc catcatcaaa ccgaacgcgg tagcaaaaaa cgtcattggt
     1441 aatatctttg cgcgctttga agctgcaggg ttcaaaattg tcggcaccaa aatgctgcat
     1501 ctgaccgttg aacaggcacg tggcttttat gctgaacacg atggaaaacc gttctttgat
     1561 ggtctggttg aattcatgac ctctggcccg atcgtggttt ccgtgctgga aggtgaaaac
     1621 gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc cggcaaacgc actggctggt
     1681 actctgcgcg ctgattacgc tgacagcctg accgaaaacg gtacccacgg ttctgattcc
     1741 gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg aaggcgaagt gtgcccgcgc
     1801 acccgttaat aatttcgtaa atgccgcgtg caaacgtggc atccgtgcgc cagaatttgt
     1861 acaatgcagc gcccccggac gagcagccgc tcaccggggc gtttcttttt tcaaccctcc
     1921 aggggccata acgtgtaata acgaggccgg aatagcatat gtctgaacaa ttagtcacac
     1981 ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct ggatctcaac cgtcagcaga
     2041 tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg cgccgatcag gtgatgaagt
     2101 ggatgtatca ctattgctgc gacaactttg atgagatgac cgacatcaac aaagtgttgc
     2161 gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga agtggttgaa gaacagcgtt
     2221 catccgacgg caccattaaa tgggcgatcg ccgttggcga tcagcgcgtc gaaacggtgt
     2281 atatcccgga agacgaccgt gccacgctct gcgtctcttc gcaggtgggg tgtgcgctgg
     2341 agtgtaaatt ctgttccacc gcccagcagg gctttaaccg caacctgcgg gtatcggaaa
     2401 ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc agcaaaagtc accggtcagc
     2461 gcccaatcac taacgtcgtg atgatgggca tgggcgagcc gctgctcaac ctgaacaacg
     2521 tcgttccggc gatggaaatc atgcttgatg atttcggttt tggcctgtct aaacgtcgcg
     2581 tgacgctttc cacttccggt gtagttccgg cgctggataa actgggcgat atgatcgacg
     2641 ttgcactggc aatttccctg cacgcgccga acgacgaaat tcgtgacgaa attgtgccga
     2701 tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt gcgccgttat ctggagaaat
     2761 ccaacgccaa tcagggccga gtcactattg aatacgtgat gcttgaccac gttaacgacg
     2821 gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga tacgccgtgt aagatcaacc
     2881 tgatcccgtg gaacccgttc ccggacgcgc cgtatggacg cagttcgaac agccgtatcg
     2941 accgtttctc aaaagtgctg atgagctacg gtttcaccac tattgtgcgt aaaacgcgtg
     3001 gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga tgttatcgac cgtacgaaac
     3061 gtactctgcg taaacggatg cagggtgaag ccatcgatat taaagcggtc tgatagccat
     3121 tacgccacgg ttactttatg taccgtggcg taatctttta tgaatgaagt attaagttgc
     3181 aattgtctgg tcaatattaa cggtgcgttt ttgctgactt taaggcagta tgtaacgatg
     3241 caacagtaag ttagcctcag tgaatgtggg ctttgtcacg agcacacaga cggtcttata
     3301 ctgtatgata acggttaact taacggatgt ttcgcggtgt gggtgagcat gatggttcac
     3361 cggcatctca attctcattt aaacgtacct gcagcgaatg aatactgaag ccacgcacga
     3421 ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat gctcgcgaac aactaggact
     3481 tagtcagcag gccgttgccg agcgactttg cctgaaggtt tccacggtac gcgacattga
     3541 agaagataag gcacccgccg atcttgcttc aacattcctg cgcggatata tccgctctta
     3601 tgcgcgtctg gtacatattc cagaagaaga actgctgcca gggctggaaa agcaggctcc
     3661 acttcgggct gcaaaagttg cgccaatgca gagtttttcc ctcggtaaac gccgcaaaaa
     3721 acgcgacggc tggctaatga ccttcacctg gctggtgttg tttgtggtta tcggcctgag
     3781 cggtgcctgg tggtggcaag accacaaagc tcagcaggaa gagatcacca ctatggccga
     3841 tcaatcttcg gcggaactga gcagtaatag cgagcagggg cagagtgttc cgttaaatac
     3901 gtcgacaact acagacccgg ctacaacgtc cacgccgcca gcgtctgtgg atactaccgc
     3961 aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc cgcaacagaa
     4021 tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc gcgaccccgg taccaacggc
     4081 agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat caggctggcg tgaccacgcc
     4141 ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc tggaggtcac
     4201 tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt aaagacggta atttgaattt
     4261 aaccggccag gcaccgtata aactgaaaat tggtgcgcca gccgcagtac agatccagta
     4321 tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg cgcgtctgac
     4381 cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag atttttcatg
     4441 cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg gaatgtgccg
     4501 attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgtac gacagatgtc
     4561 gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg gcgctgatat cgtccgtgtc
     4621 tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagcg ggttaacgtg
     4681 ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc ggaatacggc
     4741 gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg aagagcgtat tcgcatggtg
     4801 gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaacgc cggatcgctg
     4861 gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct ggaatccgcc
     4921 atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt cagcgtgaaa
     4981 gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca gatcgatcag
     5041 ccgctgcatc tggggatcac cgaagccggt ggtgcacgca gcggggcagt aaaatccgcc
     5101 attggtttag gtctgctgct gtcggaaggc atcggcgaca cgctgcgtgt atcgctggcg
     5161 gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct gcgtatccgt
     5221 tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc gtcaggaatt tgatgttatc
     5281 ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat ggacgtttcg
     5341 attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact cggcgtcacc
     5401 ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg tctggacaac
     5461 aacgatatga tcgaccagct ggaagcacgc attcgtgcga aagccagtca gctggacgaa
     5521 gcgcgtcgaa ttgacgttca gcaggttgga aaaataataa cgtgatggga agcgcctcgc
     5581 ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg ttgagggttc
     5641 atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat tcgcggcatg
     5701 aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac actgaaaaac
     5761 gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca gaccccgcta
     5821 ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta cacctttgag
     5881 gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg tgtacgcgcc
     5941 ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat cgggccgatg
     6001 ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt gggctgcgaa
     6061 gttttcggtc tgcaaggtcc ggatatcgac gctgaactga ttatgctcac tgcccgctgg
     6121 tggcgtgcgc tgggtatctt cgaacacgta actcttgagc tgaattctat cggttcgctg
     6181 gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca taaagaaaag
     6241 ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct ggattcaaaa
     6301 aatccggaag tgcaggcgct tctcaacgac gctccggcat taggtgatta tctggacgag
     6361 gaatctcgtg agcattttgc cggtctgtgc aaactgctgg agagcgcggg gatcgcttac
     6421 accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt tttcgagtgg
     6481 gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag gcggtcgtta tgacggtctt
     6541 gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg cctcgaacgt
     6601 cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt tgtcgatata
     6661 tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc tgagcgtctg
     6721 cgtgatgaat tactgggcgt gaaattgatg accaaccacg gcggcggcaa ctttaagaaa
     6781 cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg tgagtctgaa
     6841 gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac ggcagttgcg
     6901 caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag aaggacagcg
     6961 tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt tttgctgaaa
     7021 atggcaaagc actggctgtt ggggggattt tgggcgttgg cgcactgact ggctggcgct
     7081 actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat caaaatgcgg
     7141 ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa tttgctgctg
     7201 aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa tttgttgaca
     7261 aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac acgagcgatg
     7321 aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag ctcaagcagg
     7381 ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct gccattgttg
     7441 ccgacttgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg cgtagtgcat
     7501 gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg cagatgaaaa
     7561 ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact gctgccagga
     7621 ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga agatgtggta
     7681 aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc gtggagcact
     7741 tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact ggcggacaac
     7801 gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc tgaatgcgga tgatggcaaa
     7861 gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga gcctgcatta
     7921 ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga aaaggcgcag
     7981 gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt cgcgggtgaa
     8041 gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag taacggtcag
     8101 ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct cgatatgcct
     8161 tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc cgtcgtgggt
     8221 ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat ttggcagcag
     8281 cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt tgacacgact
     8341 cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct gacggcgctt
     8401 gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa tgatttcatc
     8461 gtcgatggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc gttgaccatt
     8521 gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc atcgcctgct gacttctccg
     8581 gtgctgtata atggcaacct ggtggtcggt gacagtgaag gttatctgca ctggattaac
     8641 gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt ccagactgaa
     8701 ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt gtactctatt
     8761 acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg gccgttttcc
     8821 tgtttttaac aacgacgcga atatagcgtg cgttgtctga tgatttataa aaatgaggct
     8881 ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa atccacgtta
     8941 tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg tctgactcgt
     9001 gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt ttatctgtat tgataccggc
     9061 gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc gctgctggcg
     9121 attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct gatgccggca
     9181 gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac cgaccttcct ggtggtaaac
     9241 aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct tggtttaggt
     9301 gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct ggagcatgtg
     9361 ctgctgccgt ggatggaaga tctcgcaccg caagaggaag tcgacgaaga cgctgaatac
     9421 tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggaagacga cttcgacccg
     9481 caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa gtctacactc
     9541 actaaccgta ttcttggaga agagcgcgtt gttgtttacg acatgcctgg cacgacgcgt
     9601 gacagcatct acatcccaat ggaacgcgat ggacgtgagt atgtgctcat tgacaccgct
     9661 ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctccgt aatcaaaacg
     9721 ttgcaggcca ttgaagacgc caacgtggtg atgttagtga ttgatgcgcg cgaaggtatt
     9781 tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc acttgtcatt
     9841 gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa agaaacgctg
     9901 gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc cttgcacggc
     9961 agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc cacccgtcgt
    10021 gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca ccaaccgccg
    10081 ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ctggtggtta taacccgccg
    10141 attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg attcctacaa gcgctacttg
    10201 atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat tcagttcaaa
    10261 gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca gatgcgtaaa
    10321 cgtaagcgtc tgatgaagca catcaagaaa aataaataat tatccgtaag tattttatct
    10381 gccattccct ctatgaatgt agagggaatg gctaccggag gaattcatat ggaactgcat
    10441 tgtccaaaat gtcagcatgt gcttgatcag gataacggcc atgcgcgttg cccatcctgc
    10501 ggggaattta ttgaaatgaa agctctttgt ccagactgcc atcaaccgtt gcaggtgtta
    10561 aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc atggcttgat atcgaaaaaa
    10621 cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg atttttttac cggctggatg
    10681 ttttttactt cactttctat ccagccgtct tccagacgtg tggttagcat ttcacccgtt
    10741 ttaacttgct tcactttttt cagtacattg ccgtcagtag cagtagtaac gctgtatcca
    10801 cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg ttactgcatt accgaaacgt
    10861 tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta aacgatattc cagttgctga
    10921 atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt gctgattcag ccgctgtgtt
    10981 aatcgctgct gctgttgccc ggcacgctta agctgatttt ccagcgcaaa gctcatccgt
    11041 ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc ggagctgcgg atgctgctgc
    11101 tgcaagcgat gatgaatctg cgtaaagcga cgtgtacggt tggcgagata ataatccatc
    11161 gccatctcca gccgttgacg ggccgattgc acctggcgca gtaactcttg ctgattacgg
    11221 ctcactactt cagcggcggc agacggcgtt ggcgcacgca gatcggcaac aaaatcggca
    11281 atggtcacat ccgtctcatg cccgacggcg ctgacgaccg gaatgcggct ggcaaaaatc
    11341 gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt ccagcgaacc gccgccgcgc
    11401 ccaacgatca acacgtcgca ctcattgcgc tgattcgcca gttcaatggc gcgaacaatt
    11461 tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga tgatcaccgg tagagaagga
    11521 tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag caccggtttt tgaggtgatc
    11581 acaccaacgc aatgcgcagg ggagggaagt ggttttttgt attgctgatc gaacaaacat
    11641 tcagcctgca acttcgcttt gagctgttcg tacttctgtt gcagcagccc ttcaccggcc
    11701 ggctgcatac tctcaacgat tatctggtag tcgccgcgcg gctcgtagag cgtaatattg
    11761 gcgcgaacta aaacttgttg cccatgctgc ggacggaagg tgacccggcg gttgctgttg
    11821 cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga gtgtaaagta ccagtgaccg
    11881 gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc aaacctgtcc catctcatgc
    11941 tcaagcagca gacgaaccgt ttgattcagg cgactaacgg taaaaattgc aggggattga
    12001 gaaggtaaca tgtgagcgag atcaaattct aaatcagcag gttattcagt ctatagtaac
    12061 ccgcccttcg gggatagcaa gcattttttg caaaaagggg tagatgcaat cggttacgct
    12121 ctgtataatg ccgcggcaat atttattaac cactctggtc gagatattgc ccatgctacg
    12181 tatcgctaaa gaagctctga cgtttgacga cgttctcctc gttcctgctc attctaccgt
    12241 tctgccgaat actgctgacc tcagcaccca gctgacgaaa actattcgtc tgaatatccc
    12301 tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc ctggctattg ctctggctca
    12361 ggaaggcggt atcggcttta tccacaaaaa catgtccatt gaacgccagg cagaagaagt
    12421 tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat ccgcagactg ttctgccaac
    12481 cacgacgctg cgcgaagtga aagaactgac cgagcgtaac ggttttgcgg gctatccggt
    12541 cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt cgtgacgtgc gttttgttac
    12601 cgacctgaac cagccggtta gcgtttacat gacgccgaaa gagcgtctgg tcaccgtgcg
    12661 tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac gaaaaacgcg ttgaaaaagc
    12721 gctggtggtt gatgacgaat tccacctgat cggcatgatc accgtgaaag acttccagaa
    12781 agcggaacgt aaaccgaacg cttgtaaaga cgagcaaggc cgtctgcgtg ttggtgctgc
    12841 agttggcgca ggtgcgggta acgaagagcg tgttgacgcg ctggttgccg ctggcgttga
    12901 cgttctgctg atcgactcct cccacggtca ctcagaaggc gttttgcagc gtatccgtga
    12961 aacccgtgct aaatatccgg atctgcaaat catcggcggc aacgtggcaa cagctgcagg
    13021 tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa gtcggtatcg gccctggttc
    13081 tatctgtacg actcgtatcg ttactggcgt aggtgttccg cagatcactg ccgttgctga
    13141 cgcagttgaa gccctggaag gcaccggtat tccggttatc gcagatggtg gtattcgctt
    13201 ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc gcggtgatgg taggttccat
    13261 gctggcgggt actgaagaat ctccgggtga aatcgaactc taccagggcc gttcttataa
    13321 atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa ggttcctctg accgttattt
    13381 ccagagcgat aacgctgccg acaaactggt gccggaaggt atcgaaggtc gcgtagctta
    13441 taaaggtcgc ctgaaagaga tcattcacca gcagatgggc ggcctgcgct cctgtatggg
    13501 tctgaccggc tgtggtacta tcgacgaact gcgtactaaa gcggagtttg tacgtatcag
    13561 cggtgcgggc attcaggaaa gccacgttca cgacgtgacc attactaaag agtccccgaa
    13621 ctaccgtctg ggctcctgat tctcttcgcc cgatttcatg tcgggcgatt tatattatct
    13681 gtttcacttg cctcggaata agcgtcaatg acggaaaaca ttcataagca tcgcatcctc
    13741 attctggact tcggttctca gtacactcaa ctggttgcgc gccgcgtgcg tgagctgggt
    13801 gtttactgcg aactgtgggc gtgggatgtg acagaagcac aaattcgtga cttcaatcca
    13861 agcggaatta ttctttccgg cggcccggaa agtactactg aagaaaacag tccgcgtgcg
    13921 ccgcagtatg tctttgaagc aggcgtaccg atattcggcg tttgctatgg catgcagacc
    13981 atggcaatgc agttgggcgg tcacgttgaa gcctctaacg aacgtgaatt tggctacgcg
    14041 caggtagaag tcgtaaacga cagcgcactg gttcgcggta tcgaagatgc gctgaccgca
    14101 gacggtaaac cgctgctcga tgtctggatg agccacggcg ataaagttac cgctatcccg
    14161 tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt ttgccattat ggctaacgaa
    14221 gaaaaacgct tctatggcgt acagttccac ccggaagtga ctcacacccg ccagggtatg
    14281 cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg aagccctgtg gacgccagcg
    14341 aaaattatcg acgatgctgt agctcgcatc cgcgagcagg taggcgacga taaagtcatc
    14401 ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa tgctgctgca ccgtgctatc
    14461 ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc tgcgtctcaa cgaagcagag
    14521 caggttctgg atatgtttgg cgatcacttt ggtcttaaca ttgttcacgt accggcagaa
    14581 gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg aagcaaaacg taaaatcatc
    14641 gggcgcgttt tcgttgaagt attcgatgaa gaagcgctga aactggaaga cgtgaagtgg
    14701 ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg cggcttctgc aaccggtaaa
    14761 gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc cgaaagagat gaagatgggc
    14821 ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc gtaagattgg tctggagctg
    14881 ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg gaccaggcct tggcgttcgt
    14941 gttctgggtg aagtgaagaa agagtactgt gacctgctgc gccgtgctga cgccatcttc
    15001 attgaagaac tgcgtaaagc ggacctgtac gacaaagtca gccaggcgtt caccgtcttc
    15061 ctgccagtac gttccgttgg cgtaatgggc gatggtcgta agtatgactg ggttgtctct
    15121 ctgcgtgctg tcgaaaccat cgactttatg accgcacact gggcgcatct gccgtacgat
    15181 ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga acggtatttc ccgcgtggtg
    15241 tatgacatca gcggcaagcc gccagctacc attgagtggg aatgattttg atccctgcac
    15301 tatgaatgaa caaaaccctc cgttactaca gagggttttt tatcttcaag aatcataggc
    15361 ttgaagttac taacatcgat taattaaacc agctgtccga tttgttctct tctgctttgc
    15421 ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgtttgcg tcagcttcag
    15481 attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata aattcgtttt
    15541 cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga atctctaagc
    15601 tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct tcggcgtcca
    15661 gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg ttcgcggcgg
    15721 cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc tccgcgaccc
    15781 attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct gcgacagcag
    15841 agagttgatc ctgcaatgat gaggctatag ccggatagct taaggaggct aagatggcgc
    15901 aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga catttggtat
    15961 ttggctgaat acgcgtttcg ttatacgttg tggtaataac aacggctaaa cctgtcgtaa
    16021 actggcactc tttaccctcc tgggtggagg tatacacttt ggtgccttcc ttatatgtta
    16081 aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc gcgcctacag
    16141 ctccgccgcc aactgcccct gccgtcgttc cggaattgct gccagaaccg acgttgtggc
    16201 cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg ttccgtttat
    16261 tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta acggttttag
    16321 tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga tcgtcagcat
    16381 ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga agacattttt
    16441 taaatttcat cactattcct tgttatacac attacgaaaa ataagacatt taagatcatc
    16501 aaaagaggaa agtaaatgta ttgcgtataa agggatagat taaatttatt aaaattcacg
    16561 cagggaataa tttataaaaa tatatcatta caacatatat attaatttta tatttatttc
    16621 gcagccaaac aggataaaca tcagtcagga caaagacgta aaatttaaga tgaaagaata
    16681 tctgccgctt tgataatccc aaagagcaaa agcatccgac agcgaagtgc cgttatgttc
    16741 tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga gagtgtggat
    16801 tgctgagctt cttttttgct ctgatggtgc tgccaggcac cgacggacga gtagataaaa
    16861 cggccaaaga agaagataaa gctgatgagc agtacgatac gggtcatgcg actgttaaat
    16921 cggtgtcgtt ttcgcatact agttgcctga ctcacaaaag gttccttgaa gtatgtccca
    16981 cgctgtggac ggtacttaca ttaaggcaca acaggacaaa atggtcaatt cttcgttatg
    17041 taaaaaagcg tcagtggata catattttaa tgttatggaa gttaatttaa ttatttacaa
    17101 tgatgatgta ataatgatga attaacatga taaacgtaat aattcattaa tcagggttat
    17161 tttgtttgat atatatcaat tggaactttc atatgacact tagaatcaat gctctctata
    17221 gtgatgaata atcatcattc gaagtcaggt gggatgcctg tctgaataca cctccttcag
    17281 gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata aaaatacgtg
    17341 ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt ttcgcccaca
    17401 ttaatacttt cgcgcatatt tcttccggtg aggtttttct cttttatctg cctttggcac
    17461 tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc accttaggga
    17521 tttttgtccg caaatatgca gagctgggtt tttacgaaac gctctcatta acggctaatt
    17581 ttattatcat tatcattctc tgttggggcg gttacagggt ctttactccc cggcgtaaca
    17641 acgtttcaca tggtgatacc cgtttaattt cccagcgtat attctggcag atagtgtttc
    17701 ctgcaacgct gtttctgata cttttccagt ttgctgcatt tgtaggatta ctggcgagca
    17761 gagaaaatct ggtcggtgtc atgcctttta acctcgggac tttaatcaat tatcaggcct
    17821 tactggtggg taatctgatc ggtgtcccgc tgtgctactt catcattcgg gtagtgcgaa
    17881 atccatttta tttacgtagc tattattcgc aattaaaaca gcaggttgat gccaaagtca
    17941 ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc tttattgttg ctgttatgca
    18001 tgccgttaaa tgaaaaaagc acaattttta gcaccaatta taccttgtca ttattgctgc
    18061 ccctgatgat gtggggagca atgcgctatg gttataagct gatttcgctg ctctgggcgg
    18121 tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat cctggctata
    18181 ccacgcagct gaccataacc tcctccagtt atctggtatt ctcttttatt gtcaattata
    18241 tggctgtact ggcaactcgt cagcgagcgg tagtcagacg cattcagcgg cttgcgtatg
    18301 tggacccggt ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgttg
//
LOCUS       DAJTPG010000080        18037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTPG010000080 DAJTPG010000000
VERSION     DAJTPG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            431..1114
                     /locus_tag="OO230_002837"
     CDS             431..1114
                     /locus_tag="OO230_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5692526.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1158..5820
                     /locus_tag="OO230_002838"
                     /pseudo
     CDS             1158..5820
                     /locus_tag="OO230_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6188..7843
                     /gene="lldP"
                     /locus_tag="OO230_002839"
     CDS             6188..7843
                     /gene="lldP"
                     /locus_tag="OO230_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5692527.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7843..8619
                     /gene="lldR"
                     /locus_tag="OO230_002840"
     CDS             7843..8619
                     /gene="lldR"
                     /locus_tag="OO230_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5692528.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8616..9806
                     /gene="lldD"
                     /locus_tag="OO230_002841"
     CDS             8616..9806
                     /gene="lldD"
                     /locus_tag="OO230_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5692529.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10002..10475
                     /gene="trmL"
                     /locus_tag="OO230_002842"
     CDS             10002..10475
                     /gene="trmL"
                     /locus_tag="OO230_002842"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5692530.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10528..11349)
                     /gene="cysE"
                     /locus_tag="OO230_002843"
     CDS             complement(10528..11349)
                     /gene="cysE"
                     /locus_tag="OO230_002843"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5692531.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11429..12448)
                     /gene="gpsA"
                     /locus_tag="OO230_002844"
     CDS             complement(11429..12448)
                     /gene="gpsA"
                     /locus_tag="OO230_002844"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5692532.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12448..12915)
                     /gene="secB"
                     /locus_tag="OO230_002845"
     CDS             complement(12448..12915)
                     /gene="secB"
                     /locus_tag="OO230_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5692533.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12978..13229)
                     /gene="grxC"
                     /locus_tag="OO230_002846"
     CDS             complement(12978..13229)
                     /gene="grxC"
                     /locus_tag="OO230_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5692534.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13371..13802)
                     /locus_tag="OO230_002847"
     CDS             complement(13371..13802)
                     /locus_tag="OO230_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5692535.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14047..15591
                     /gene="gpmM"
                     /locus_tag="OO230_002848"
                     /gene_synonym="pgmI"
     CDS             14047..15591
                     /gene="gpmM"
                     /locus_tag="OO230_002848"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5692536.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15601..16884
                     /gene="envC"
                     /locus_tag="OO230_002849"
     CDS             15601..16884
                     /gene="envC"
                     /locus_tag="OO230_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5692537.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16888..17847
                     /locus_tag="OO230_002850"
     CDS             16888..17847
                     /locus_tag="OO230_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5692538.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17834..18037)
                     /locus_tag="OO230_002851"
                     /pseudo
     CDS             complement(17834..18037)
                     /locus_tag="OO230_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4544 a         4659 c         4674 g         4160 t
ORIGIN      
        1 cttcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata gccatctata
       61 aacaataaaa atatatagaa gatatcattt gttaattaac aagcatttac gtctcttttt
      121 ttcggattta attgtgaaat tttaatcatc atattcacaa aatcaattat ctgtgaaatt
      181 tttcccagac atggtttctt tcatatttga catttattaa taatcatcat tagaatccac
      241 gaaaaataat atattcatac aaacacagac atcttttgaa acgaatgata catttttatg
      301 atgaatgctt aaaacaatat ttaatcgaca cacatcactg tcattaatgt aaagcaacat
      361 agctcaccaa taatacaatg gacaactcta ccggcattga gcgctggtcg gaggaaataa
      421 ggatttttac atgaacttga agaagatttt tttcagtgct gtcactgttt cagttctctg
      481 cgcattaacg ggatgtgact acattgaaga aggcaagcca gaaagtagtt tactgaaaca
      541 acaagaagaa cataataata aaattgtgct acttgaaaaa caacaagcac agctaaagag
      601 tcaactcgaa actattcaaa aacaacaaac tggcataata aacagtacaa agactttaac
      661 tcacgtaatt aaatccgtta aagaccaaca aaatactttt atttttaccg agtttaaccc
      721 agcaaaaacg aagtatttca tcctgaacaa cggttcggtg gctttagcgg gtcgagtgtt
      781 atccattgac gccacagaga atggtagtgt tattcatatc tcactggtca acttattaag
      841 tacacctatc tcaaacattg gttttaatgc gacatggggt ggtgaaaaac ctgtcgatgc
      901 caaagagttt gcccggtggc aacaattact tttcaacaca tcaatgaaat ccacattgaa
      961 attattacca ggtcaatggc aagacattaa tttgaccctg aagggtgtat cgcccaataa
     1021 cctgggatat ctgaagttag ccatcaacat ggaaaatatt cagttcgaca atcttccctc
     1081 tgctgaaaat cggcagaaaa gaagcaaaaa ataaatgcag ttagtacgct tataacaagg
     1141 aatagcgagc atcattaatg aacaaaatat ttaaagttat ctggaaccct gcgacaggga
     1201 attatactgt taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg cgcagtaagc
     1261 tgttaatttc tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca ttggcgaatg
     1321 ccgggaatga caacggtcag ggtgttgatt acggtagtgg atcagctggc gacggctggg
     1381 ttgctatagg caaaggggcg aaagcaaata cttttatgaa caccagtggt tccagtactg
     1441 ctgtgggtta tgacgctata gctgaaggcc aatatagctc tgccatcggg tcaaaaaccc
     1501 atgcgattgg tggtgcatca atggcctttg gggttagtgc aatatcagaa ggcgatagaa
     1561 gtatagcact gggtgcctct tcgtattcat tgggccaata ctcaatggcc ctcggccgtt
     1621 attcaaaagc attgggtaaa ttgtctattg ctatggggga ctcttccaaa gcggaaggag
     1681 caaacgccat tgccctgggg cgtagcagtg tagctagcgg tactgacagc ctcgcatttg
     1741 gcagacaatc acttgccagc gcagcgaacg ctattgcgat aggtgctgag accgaagccg
     1801 ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa agggactaat agcatggcaa
     1861 tggggttcgg aagccttgcc gataaagtca atactatcgc attaggaaat ggcagccagg
     1921 ctctggcaga taatgcaatc gccataggcc agggcaacaa agctgatggc gtggatgcca
     1981 tcgctctggg taatggtagc cagtcgagag gcttaaacac cattgcctta ggcacagcca
     2041 gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa tagcagtgcc aacggtatta
     2101 actctgtcgc gctgggcgca gattccattg cggatttaga caataccgtc tctgtcggca
     2161 atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg cgcgatcaag tctgacagtt
     2221 acgatgccat taatggttca cagctttatg ccattagcga ctcggtagca aaaaaggctt
     2281 ggaggagggg ctgcagtaga tgttgatgac ggtactgtta cagcaccaac ctacaattta
     2341 aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg tacttgatga aaacaccctg
     2401 caatgggacc aaaccaaagg caaatacagc gctgctcatg gtactagtag cccaactgcc
     2461 agcgtaatca ccgatgttgc ggatggcacg atttcagcct ccagtaagga tgcggttaac
     2521 ggttcccaac tgaaagctac caatgacgat gtcgaagcca acaccaccaa tatcaccaac
     2581 ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga aactaaaaag
     2641 gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt taaagatgcc
     2701 gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac caccaacgat
     2761 gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc cactaacacc
     2821 accaacatct ctaacctgac tgagacggtg actaatcttg gtgaggatgc gctgaaatgg
     2881 gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag caaaataacc
     2941 aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg ctctcagctg
     3001 aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa caccaccaac
     3061 atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag
     3121 gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat caccaatatc
     3181 ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca gctttatgac
     3241 ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg
     3301 tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg cgatgcactg
     3361 gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg ggatgccacc
     3421 gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat cactgatgtc
     3481 gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca actccacggc
     3541 gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc agacggcacc
     3601 atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg tgatgccctg
     3661 aatgctatcg ataccactcc tgacgacgct ctgctctggg atgcggacgc cggtgaaaat
     3721 ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac taacgtcgct
     3781 aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact ctataccacc
     3841 aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga cggcaccatc
     3901 accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga cgccctggat
     3961 gcgcttgatg ataacgccct gctgtgggat aagactgcca atggcggtgc tggagcctac
     4021 aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg cagtattagt
     4081 gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat gatgattgag
     4141 cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta tatcgaagaa
     4201 aatggcgctg gtatcaacta cgttcgtact aacgacaacg atttagcctt taacgatgca
     4261 agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc tggtgccagc
     4321 agcgtagcca ttggtcagaa cagcagcagc accgttgata ccggtattgc gctgggtagc
     4381 agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt aacggaaaat
     4441 ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt gtcaattggt
     4501 gatgacggta aataccgcca aatcatcaac gtagctgatg gttcagaagc ccatgacgcc
     4561 gttacggttc gccagttgca gaacgctatt ggagcggtcg ccactacgcc aaccaagtac
     4621 tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc gctggcaatg
     4681 ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa cactctggtt
     4741 ctggctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa ccatgcaaac
     4801 agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg ctacaccgcc
     4861 tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgtcggtag tgaagacggt
     4921 caacgtcaga tcacaaacgt cgcagctggt tcagcggata ccgatgcggt taacgtgggt
     4981 cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac taacctgaac
     5041 aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat tggcgacatt
     5101 gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga tgccaacgcc
     5161 cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga caacagcgtc
     5221 gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg ttcttctact
     5281 aaccaacgcc gtatcaccaa cgtagctgcc agtgttaatg ccaccgatgc ggttaacgtg
     5341 tcgcagctga aatcttctga agcaggcgga gtgcgctacg acaccaaagc tgatggttct
     5401 atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg tatcagcaac
     5461 gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa gcaaagcgcg
     5521 caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa actgtctaaa
     5581 actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg tctgccgcag
     5641 gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa cggtgaatcg
     5701 gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta caaattacaa
     5761 ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat tcagtggtaa
     5821 tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc gttacgcgca
     5881 tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga tgaggattgc
     5941 ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc ttcgcttatc
     6001 tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa tacataacat
     6061 ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc cacttttaca
     6121 caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg actctaggag
     6181 acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct ggctttccag
     6241 tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc tcaaactgaa
     6301 aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt tgctgttcta
     6361 taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct atggtttgtg
     6421 gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg tgaaaaccgg
     6481 gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc gtctgcaaat
     6541 gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct ttggcgcacc
     6601 ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt acgccgccgg
     6661 actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca ttccaatcct
     6721 ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg tggggcgtca
     6781 gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg acggctggcg
     6841 cggtatcaaa gagacgtggc ctgcggtcgt ggttgcaggc ggctcgtttg ccatcgctca
     6901 gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt cgctggtatc
     6961 actgctctgc ctgacgctgt tcctcaaacg ctggcaacca gttcgcgtat tccgcttcgg
     7021 tgacttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca ccgcgggtca
     7081 ggtgttacgt gcctggacac cgttcctgtt cctgaccgct accgtaacac tgtggagcat
     7141 cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg tgatcaacat
     7201 tccggtgccg tacctcaata aactggttgc ccgtatgcca ccagtggtca gcgaggctac
     7261 agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg ccattctgtt
     7321 tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta tcagcacctt
     7381 cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg tgctggcgtt
     7441 cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac tggcgcacac
     7501 cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat tcctgaccgg
     7561 gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag cacaacaaat
     7621 tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca ccggtaagat
     7681 gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg gcaaagagtc
     7741 tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg tgggcgtgat
     7801 caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt tttacccaga
     7861 cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa
     7921 gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat
     7981 tcactacgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag tcgacgcggc
     8041 ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat cgtccagccg
     8101 ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga agcccgctac
     8161 gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa
     8221 aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc ctcacaagcg
     8281 gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct gctgcaaacc
     8341 atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca gcggatgtat
     8401 ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat tgacgccatt
     8461 tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag ttttgttcac
     8521 accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac ccgcctgccc
     8581 ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca gccagcgatt
     8641 atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg gatggtggtg
     8701 catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg gcactgcgcc
     8761 agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt aatgagaaat
     8821 tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg cgtcgtggcg
     8881 aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc tcgacggttt
     8941 ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg ttccagcttt
     9001 atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa gcagcgggtt
     9061 gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac cgtgatgcgc
     9121 attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg gtgacgcatc
     9181 cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt aatatctcag
     9241 cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg aataacttcg
     9301 atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat ggcccgatgg
     9361 tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt ggtgctgatg
     9421 gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct tccgctcgtg
     9481 cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg gatagcggaa
     9541 ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc gtactgctgg
     9601 gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct aacctgctaa
     9661 atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa tcgatcagtg
     9721 aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctacg gcactggctc
     9781 cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tcaccctaac cctctccctc
     9841 agggagaggg gaccgttcgg cgctgtatgt actccctcac tctgaaacga caccgcactc
     9901 tttttttctc cctcgcccct ccggggaggg ccggggtgag gggaaaaggc cgcacggttc
     9961 accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac atcgtacttt
    10021 acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct aataccggct
    10081 ttcgtctaca tatcatcgaa ccgatgggat ttgcctggga cgacaagcgc ctgcgccgcg
    10141 cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat cgcgcgttcc
    10201 tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg cctgctcaca
    10261 gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca cgcggcctgc
    10321 cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgcattccg atggtgccgg
    10381 acagccgcag catgaatctg tccaatgcgg tgtcggtagt ggtgtatgaa gcctggcggc
    10441 agctgggata tccgggagcg ttgttgagag attagttact gtatgccgga tagatgatta
    10501 catcgcatcc ggcacgatca caggacatta gatcccatcc ccatactcaa atgtatggtt
    10561 aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg gtttaccgac
    10621 aatacgagcc ggaacgccag cggcggttgt atgcggcggc accggttgca gcaccacgga
    10681 acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg ccgaggattt tcgcgcccgc
    10741 gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt tacccgtacc
    10801 gccaagcgtc acagattgca gaatcgatac gtcgttttca attaccgccg tttcacccac
    10861 aacgatgccc gtggcgtggt cgagcataat gccacggcca atttttgctg ccgggtgaat
    10921 atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg cgcgacgccc
    10981 ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac ccttcaggta
    11041 taacaacggg gttgagtatt tatcgactgc cgggtcgcgg gtacgcaccg cctgaatatc
    11101 acaggccgca gaggcgatca tttccgggtc agcggcgtag gcttcttcaa ccacttcacg
    11161 gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt agctcagtgc
    11221 actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca gcattggctc
    11281 acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt ccagttcttc
    11341 acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc gcgggccggg
    11401 tcatagcggt aacaaaggtt ccctggggtt agtgactgct gcgctcgtcc ttgcgtgcac
    11461 gacctaataa tgtcaatgct gcctcgcgcg cgttttttcc gcaatataat acttgataaa
    11521 tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg acttctttcg
    11581 tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt acatccatgc
    11641 cctgaccgag catcatacca aaacggcggt tacgcgactg gttgtcggta caggtaagca
    11701 ccagatcgcc cagccccgcc atgcccataa aggtggcagg atcggcaccc agcgccgcac
    11761 caagacgcga catttcagcc agcccacggg tgatcagcgc cgtacgcgca ttcgcaccaa
    11821 aaccgatacc gtcagacatc cccgcgccaa tggcaatgac gtttttcacc gcgccgccaa
    11881 gctgcacgcc aatgaaatcc gggttgctgt aaacgcggaa acttttgccg cagtgcagca
    11941 gctgctggag atcatcggca aaggtctgat cggtcgaggc cagcgaaata gctgtcggta
    12001 aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc ggaatttgat
    12061 cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttccgct tccagccctt
    12121 tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg cgcagcactt
    12181 caccaaagac atggctgggt acgacgacga gaatattacg actggctgcc agcgcagtgg
    12241 cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga aacgcggcgt
    12301 tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag aggacaacct
    12361 cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg gcaccgatca
    12421 cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag taccttcgcc
    12481 agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg caaggttcag
    12541 ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat acgggaacag
    12601 aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga tacccgcgat
    12661 ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc ccaaggaggc
    12721 cgttacggta acacgcagta ccacttcgta tacgtcatct gccagttggg aagatgccgt
    12781 atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg gcgcgttcgg
    12841 cgcttcgaaa gagatatcct tggtataaat acgttggatc tggaaagtca tttcagtgtt
    12901 gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact gcgcaatgta
    12961 ataccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc atccaatgca
    13021 tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg aaccgtggtg
    13081 caaccgctgc gtttgatcat ctcttcacgc ttggcggcat tgccatcgat cggcaactcc
    13141 tggaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata cgggcaggtt
    13201 tctttggtat agatttcaac attggccata atttagctcc tgttttttta ccctgaaatt
    13261 tcatgttgca gggcggctgc aaaatggagt gtccccagaa gcttataaca ataagtcgct
    13321 gggggcctaa ttgcagctaa cggcctgcat catgaaagac gacaggtaaa ttatttgccg
    13381 cgcaccagag gcaagttttc gccagcccag ccagcaacgc cctctttcag cacgaatact
    13441 tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct ggcactgcat gccagaaccg
    13501 tctaccacga taaccgattt gtctttgtgc ttctcaagct caccaacatt gttggctttg
    13561 atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa gtcatcacgc
    13621 tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc ttcaccacga
    13681 gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac cgccaccagt
    13741 aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat aatttcttgc
    13801 atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag tatacctgcg
    13861 cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg cacgacgaaa
    13921 aaaaagcgca aatctggcat cggcttgccg cgagcggtcg tatattttga tcttcagagg
    13981 ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga gtatgaggtt
    14041 gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg ctatggctat
    14101 cgtgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat ggatgcactg
    14161 tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg tctgcctgac
    14221 cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg catcgtgtat
    14281 caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc taatccagtg
    14341 ctggctggcg cggtacataa agcgaaaaac gcaggtaaag cagtacacat tatgggtctg
    14401 ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt agaactggca
    14461 gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg cgacaccccg
    14521 ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga aatttgctgc gctgggtaaa
    14581 ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa ccgttgggat
    14641 cgcgttgaaa aagcctatga cctgctgact ctggcgcagg gtgagttcca ggccgatact
    14701 gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt cgtgaaagcg
    14761 accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga tgcgctgatt
    14821 ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc gtgcattcgt gaacgctgat
    14881 ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct gaccgaatac
    14941 gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa caccttcggc
    15001 gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga aaaatatgcc
    15061 cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga agatcgcatt
    15121 ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag ctccgcagag
    15181 ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat tatctgtaac
    15241 tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt taaagcggtt
    15301 gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt gggtggacaa
    15361 ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc aacgggtcag
    15421 gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga taagaacgtg
    15481 aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc gctgatgggt
    15541 atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata atccctcccc
    15601 atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag gtttgcaatc
    15661 aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc cttttccgcc
    15721 cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccgc aaaagagcgc
    15781 gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa aaagcaggaa
    15841 gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct caatcaactc
    15901 aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca aaaagccgcc
    15961 caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga gcataccggt
    16021 attcagctaa ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca ggcttatttc
    16081 ggctatctca accaggcgcg acaagaaacc attgcccagt tgaagcaaac gcgtgaagaa
    16141 gtcaccatgc agcatgctga actggaagag aaacaaagcg agcaacaaac gctgttatat
    16201 gaacagcgcg cccaacaggc gaaactgact caggctctga acgagcgtaa aaagacgctg
    16261 gcggggctgg aatcttccat ccagcaaggt cagcagcagc taagtgaact gcgtgccaac
    16321 gaatcccgtc tgcgtaacag cattgcccgt gctgaagccg cggcgaaagc gcgtgctgaa
    16381 cgagaagcgc gtgaggccca ggcggttcgc gagcgccaga aagaagcgac gcgcaaaggc
    16441 accacctaca aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg tggcctgggt
    16501 gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga cgctgcatcg ctatggcgag
    16561 cagctacagg gtgaattacg ctggaaaggg atggttatcg gtgcctctga aggtactgaa
    16621 gttaaagcaa ttgccgacgg tcgagtgatt ctggctgact ggctgcaagg ttacggtctg
    16681 gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt acggctataa tcagagcgca
    16741 ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa ttgcactggt gggcagcagt
    16801 ggcggtcagg gtcggccttc actctatttc gaaattcgcc gccagggtca ggcggtcaat
    16861 ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt tcttgcattt
    16921 gccgctctgt tggcgctctc ctcccccgta cttgctggca aacttgccat cgtcattgat
    16981 gattttgggt atcgcccgca caacgaaaac caggtgctgg cgatgccttc cgctatctcc
    17041 gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc gcataacagc
    17101 ggtcacgaag tgttgattca tctcccgatg gcaccgttga gtaaacagcc gctggagaaa
    17161 aatacgctac gcccggagat gagcagcgac gaaattgagc gcattattcg tagtgcggtc
    17221 aataacgtgc cctatgccgt ggggatcaac aaccacatgg gcagcaagat gacctctaac
    17281 ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt acaatcttta cttcctcgac
    17341 agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc aaggtactgg cgtgaaggtg
    17401 atcaaacgga aggtgttcct cgacgattcg cagaacgaag cggacatccg tgtgcaattt
    17461 aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca ttgccattgg gcatcctcac
    17521 ccttcaacgg tacgcgtgct acaacagatg gtttataacc tgccgccaga cattacgctg
    17581 gtgaaagcca gcagcttgct gaatgaaccg caggttgata cttctacacc gccgaaaaac
    17641 gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga agctgtgcaa accgaagaaa
    17701 ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag catcagccag
    17761 agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca gcccgaagcg
    17821 gcgaagttta acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg tgcgaccgcg
    17881 ttttgtcgcg ccattgccat aagcggaaag accacagtaa cgcctggtaa ccgaccttca
    17941 cgctgcgcac attggtaatc aggcgcttat acatacccgg aagtaaatat ctcggcaatc
    18001 atccgttggc gggtaagaat atccggctct ttgcgca
//
LOCUS       DAJTPG010000081        17604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTPG010000081 DAJTPG010000000
VERSION     DAJTPG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..482
                     /locus_tag="OO230_002852"
     CDS             <1..482
                     /locus_tag="OO230_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5692539.1"
                     /translation="MSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPT
                     TVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTV
                     GDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
     gene            563..1237
                     /locus_tag="OO230_002853"
     CDS             563..1237
                     /locus_tag="OO230_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5692540.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1253..3733
                     /locus_tag="OO230_002854"
     CDS             1253..3733
                     /locus_tag="OO230_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5692541.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3749..4782
                     /locus_tag="OO230_002855"
                     /pseudo
     CDS             3749..4782
                     /locus_tag="OO230_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4864..5202)
                     /gene="rcnB"
                     /locus_tag="OO230_002856"
     CDS             complement(4864..5202)
                     /gene="rcnB"
                     /locus_tag="OO230_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5692542.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5421..6242)
                     /gene="rcnA"
                     /locus_tag="OO230_002857"
     CDS             complement(5421..6242)
                     /gene="rcnA"
                     /locus_tag="OO230_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR5692543.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRGERNWLENMHGHDYEHHHHHHDHEHHHDQGHHHHHEHGEYQDAHARAHANDI
                     KRRFDGREVINWQILLFGLTGGFIPCPAAITVLLICIQLKALTLGATLVVSFSIGLAL
                     TLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6363..6635
                     /gene="rcnR"
                     /locus_tag="OO230_002858"
     CDS             6363..6635
                     /gene="rcnR"
                     /locus_tag="OO230_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5692544.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6727..6823
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6858..7646
                     /gene="thiM"
                     /locus_tag="OO230_002859"
     CDS             6858..7646
                     /gene="thiM"
                     /locus_tag="OO230_002859"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5692545.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7643..8443
                     /gene="thiD"
                     /locus_tag="OO230_002860"
     CDS             7643..8443
                     /gene="thiD"
                     /locus_tag="OO230_002860"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5692546.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8508..9329
                     /locus_tag="OO230_002861"
     CDS             8508..9329
                     /locus_tag="OO230_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5692547.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9377..10123
                     /locus_tag="OO230_002862"
     CDS             9377..10123
                     /locus_tag="OO230_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5692548.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10097..11062)
                     /locus_tag="OO230_002863"
     CDS             complement(10097..11062)
                     /locus_tag="OO230_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5692549.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11059..12062)
                     /locus_tag="OO230_002864"
                     /pseudo
     CDS             complement(11059..12062)
                     /locus_tag="OO230_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12102..13154
                     /gene="fbaB"
                     /locus_tag="OO230_002865"
     CDS             12102..13154
                     /gene="fbaB"
                     /locus_tag="OO230_002865"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5692550.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13462..14316
                     /gene="gatY"
                     /locus_tag="OO230_002866"
     CDS             13462..14316
                     /gene="gatY"
                     /locus_tag="OO230_002866"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5692551.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14345..15607
                     /gene="gatZ"
                     /locus_tag="OO230_002867"
     CDS             14345..15607
                     /gene="gatZ"
                     /locus_tag="OO230_002867"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5692552.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15617..16069
                     /gene="gatA"
                     /locus_tag="OO230_002868"
     CDS             15617..16069
                     /gene="gatA"
                     /locus_tag="OO230_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5692553.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16100..16384
                     /gene="gatB"
                     /locus_tag="OO230_002869"
     CDS             16100..16384
                     /gene="gatB"
                     /locus_tag="OO230_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5692554.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16388..>17604
                     /locus_tag="OO230_002870"
     CDS             16388..>17604
                     /locus_tag="OO230_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5692555.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPIT"
BASE COUNT         4586 a         4036 c         4502 g         4480 t
ORIGIN      
        1 ttatgagcgc tggagtattt gctgcagacg ttgataccgg aacattaacc attaaaggaa
       61 atattgcaga atctccgtgt aaattcgaag cgggtggtga ttcagtaagt attaatatgc
      121 cgactgtacc aaccactgtc tttgaaggta aagctaaata ttctacctat gatgatgcag
      181 tcggtgtaac cagcagcatg ttaaaaatta gctgcccgaa agaagttgct ggtgtaaaac
      241 tctcgttgat cacaaacgac aaaataaccg gtaacgataa ggcgatagcc agtagcaacg
      301 ataccgtagg tgataacagc gatgtcctag atgtttctgc accttttaac attgagagtt
      361 ataaaacagc ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag
      421 ataactcagt gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt
      481 aataaataaa attgcaagtt tctccaggta ataaacatgg ccgctatccc catggcggct
      541 ttttaattta agaggcatta agatgaaagg attattatct ttactcattt tttctatggt
      601 ccttcctgca catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa
      661 taaagaagtt atggtgcagt tgatgaacca gggaaatcgt tcttcgctgc tgcaggcgtg
      721 gattgatgat ggcgatacgt cgttaccacc agaaaaaatt caggttcctt tcatgttaac
      781 gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc
      841 gaataaactg cccactaata aagaaagcat tttttatctg aatgttctgt atattccacc
      901 aaatagtcca gagcaagaag gcaagaatgc actgaagttt gcgatgcaaa acagaattaa
      961 gttgttttac cggccagcgg gtattgctcc ggtaaataaa gcgaccttta aaaagttact
     1021 ggtaaatcgc agtggcaatg gattggtgat aaaaaatgac tcagctaatt gggtgacgat
     1081 ttcggatgtc aaagctaata atgttaaagt caattatgaa actattatga ttgccccctt
     1141 agaaagtcag agtgttaata tcaaaagtaa taatgcaaat aactggtatc tgaccattat
     1201 caatgaccac ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
     1261 aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc
     1321 tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa
     1381 tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa
     1441 taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt
     1501 atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca
     1561 atgtttaaca tttgagcaac ttgttcaggg tgggagctat agctgggata tcggggtttt
     1621 tcgtctcgat ttcagtgtcc cgcaggcttg ggtggaagaa ctggaaagtg gctatgttcc
     1681 accggaaaac tgggagcggg gtattaatgc gttttatact tcttattatg tgagtcagta
     1741 ttacagcgac tataaagcgt cgggtaacaa caagagtaca tatgtacgtt ttaacagcgg
     1801 gttaaattta ctggagtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa
     1861 tccaggggtg tggaaaagca ataccctgta tctggaacgt ggatttgccc aatttctcgg
     1921 cacgcttcgc gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag
     1981 cggtgtgcgg ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa attttacgcc
     2041 acgggtgcag gggattgctc agagtaacgc gctggtaact attgaacaga atggttttgt
     2101 ggtttatcag aaagaggttc ctcctggccc gtttgcgatt acagatttgc agttggccgg
     2161 tggtggagca gatcttgatg ttagcgtgaa agaggcggac ggctcggtaa ccacctatct
     2221 ggtgccttat gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat atgattttgc
     2281 ggcgggccgt agccatattg aaggggcgag caagcaaagt gattttgtcc aggcaggtta
     2341 tcagtatggt tttaataact tattgacgct gtatggcggc acgatggttg ctaataatta
     2401 ctatgcgttt accctcggaa cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc
     2461 cacgaagtcg catagtaaac aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat
     2521 tgcctacaac aaatttgtga gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta
     2581 ttcgtcgcgt gattaccgga catttaacga tcacgtttgg gcaaacaata aagataatta
     2641 tcgccgtgat gaaaacgata tctatgacat tgccgattat taccagaacg attttggccg
     2701 taaaaatagc ttttctgcca atatgagcca gtcattgcca gaaggctggg gttctgtgtc
     2761 cttaagtacg ttatggcgag attactgggg gcgtagcggc agcagtaagg attatcagtt
     2821 gagttattcc aataactggc ggcggataag ctataccctc gcggcaagcc aggcgtatgg
     2881 cgagaatcat catgaagaga aacgttttaa tatttttata tcaattccct gtgattgggg
     2941 tgatgacgtt acgacgcctc gtcggcaaat atatatgtct aactcaacga cgtttgatga
     3001 tcagggtttt gcctcaaata atacgggatt atcaggaaca gtagggagtc gggatcagtt
     3061 caattatggt gtcaacctga gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa
     3121 tttgacctgg aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt cgagtactta
     3181 tcgacagact ggagccagtg tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct
     3241 ggcgaaccgt ctttccgaaa cgtttgctgt gatgaatgcg ccaggaatta aagatgctta
     3301 tgtcaatggg caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat acgacggaat
     3361 gacaccttat cgggaaaatc acctgatgct ggatgtgtcg caaagcgata gcgaagcaga
     3421 attacgtggc aaccggaaaa ttgccgcccc ttatcgcggc gcggttgtac tggttaattt
     3481 tgataccgat cagcgcaagc cctggtttat aaaagcgtta agagcggatg gtcaaccatt
     3541 aacgtttggt tatgaagtca atgatatcca tggtcataat attggcgttg tcggccaggg
     3601 aagccagtta tttattcgca ccaatgaaat accgccatcg gttaatgtag caattgataa
     3661 gcaacaagga ctttcatgca caatcacctt cggtaaagag attgatgaaa gtagaaatta
     3721 tatttgccag taatagtcag gtggttctat ggaaattcgc ataatgctat ttatattaat
     3781 gatgatggtt atgcctgtga gctatgcggc atgttatagt gagttatctg ttcagcacaa
     3841 tttggttgtt caggggaatt ttgcacttac tcaaacacaa atagcgacat atgagcataa
     3901 ttttaatgat tcgtcatgcg tgagtacaaa tactatcacc cctatgagcc catcggatat
     3961 tattgttgga ctttataacg ataccattaa attaaattta cattttgaat ggaccaataa
     4021 aaacaacatc acgttgtcaa ataatcagag cagtttcacc agtggttatt cggtaacggt
     4081 gacacctgcg gccagtaatg caaaagtgaa tatttctgcg gggggcggtg gttcagtgat
     4141 gattaatggt gttgcgacat tatccagtgc ttcatcatcg acacgcggga gtgccgcagt
     4201 acaatttcta ctgtgtttat taggtggcaa gtcatggggt gcatgtgtaa atagctacag
     4261 aaatgcattg gcacaaaatg caggtgtcta ttccttcaat ctgacattgt catacaaccc
     4321 gataaccaca acctgcaaac aggacgattt attaattact ttagacagta ttcccgtttt
     4381 acaattacca gccacaggta acaaagcaac aataaatagt aaaaaaaggg atattattct
     4441 gcgttgtaaa aatttattag gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt
     4501 atcaagttct gacttgttaa ccaacagcaa cacaatactg aaaggtgcgg aagataatgc
     4561 gtaggattta ttcttgaaag taatggttcg ccagtcacac ttttaaatat cactaacagc
     4621 agtaaaggat acacaaattt aaaagaaatt gcggcaaagt caaaacttac agatacaacg
     4681 gtttcaattc cgataacagc cagttactac gtctacgata caaacaaagt taaatctggc
     4741 gcactggagg caaccgcatt aatcaacgtg aaatacgact aataacaagc aaaacgagca
     4801 agtggctaat aataaaaaat aacaaggtgt ctgcatttcc ccctcatgat gagggggctt
     4861 tttttagcga tgataaaaaa tctcaccgtc gtaggcttta atgattttac cgtcggtgtc
     4921 gctgatcaac acgtacgcgc cacccatata ggtccagtgc gtcccggcat caggcgcggg
     4981 caggttacgt aactgccact gcttaatgtt gtactcatcg gtgcggtaca tctctgggac
     5041 ggtatcacct ggtttgaaat gcttaaagtc agcaatgaaa ctacttaact catacttaga
     5101 aattcccgag gtattggtcg aacccgcggt ggctggtgcg gcccaggcgg cactggtaac
     5161 cagcaaaagc acacccagca acatcttatt tttaatggtc ataaactctc cactttgggg
     5221 atctgtaata tcagtcgcat atctttcctg tcatacaata taactgacaa gcgatatttt
     5281 gttagtgatc ccatgatttc gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt
     5341 agcgagcaga gagtcagcgg aaatagctga tttaagaatc atcctgggct gaagataaca
     5401 tgcaagccta aaggattgag ttatcgcatt atgcccatga agccatgtac gcccatatac
     5461 acaccgacta agccaatcaa cagactggaa aaataggggg cgcgtttagc gagagtgtta
     5521 aatccgctcc agcgttttgc gacctgctga acgctgattg ctgcgccaac gcctacggtg
     5581 acaagcgtta acgccaggcc aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg
     5641 gctttcaact gaatgcatat caacagcacg gtaattgctg ccgggcaggg gataaagcca
     5701 ccggttaagc caaataacaa aatttgccag ttgatgacct ctctaccatc aaagcgtcgt
     5761 ttaatgtcat tggcatgggc tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg
     5821 tgatgatgtc cttggtcgtg gtgatgttcg tgatcgtgat gatggtgatg atgctcataa
     5881 tcatgcccgt gcatattctc cagccagttg cgttcgccgc gccagaacat ccagaacgcg
     5941 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     6001 aagcgcttac tgatcaccat cccgccaaag gcaatcaacc agaccaccgc ggtatgcgaa
     6061 atggttgctg ccagtccgag catcaccgcc tgtttaatgg tgcctttgat ggcgatgata
     6121 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     6181 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     6241 atgataataa ttcttagtat taattcggca atctgattct actccccccc agtacctgat
     6301 actacccccc agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg
     6361 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     6421 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac
     6481 aacagattgc tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc
     6541 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     6601 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     6661 cgtttttcct cgtttacaac gggggcgctg gacattacca tcctcctctg cgatttatca
     6721 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     6781 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     6841 tatggcagga gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt
     6901 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     6961 cctttaccgc caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     7021 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     7081 cgcagccacg cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     7141 gctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     7201 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     7261 ctggcattgc taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     7321 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     7381 attatgttac cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca
     7441 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     7501 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg
     7561 cagtcgccag aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc
     7621 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     7681 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     7741 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     7801 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     7861 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     7921 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     7981 cgacccgctg ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt
     8041 ttcattaata acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg
     8101 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     8161 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     8221 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg
     8281 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     8341 acaggaggca aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     8401 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     8461 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     8521 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     8581 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     8641 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt
     8701 acaccattca cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg
     8761 caaaaatgcg cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg
     8821 aaaagctggt ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc
     8881 tgcgcggggc gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat
     8941 ttctgcaaac ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac
     9001 ggggaaaatt atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg
     9061 aaaaacgcac gggaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc
     9121 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg
     9181 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     9241 tcaatggtcc ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt
     9301 tgatgggatt aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct
     9361 ggcaggaaaa agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg
     9421 tattttagcg gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc
     9481 cgtgcgcagc ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca
     9541 gttaaccggc gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg
     9601 cgtggtgacg cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt
     9661 gaaagaagcg agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct
     9721 atgggttaat aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggtcga
     9781 agcgggaagt gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc
     9841 gattgaggaa tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc
     9901 tctttatgac tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag
     9961 cgcccggatg ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt
    10021 gctggtgatc aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag
    10081 ccactgtcgc agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg
    10141 cacaatcacc gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg
    10201 catccgccag tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc
    10261 ccgccccaat ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt
    10321 tgctgaaata ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct
    10381 cctgccattg cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa
    10441 tctcagcctc ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta
    10501 atgcgtccgg gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca
    10561 gcttttccag ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa
    10621 aatagagcag gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat
    10681 tttccacacc gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac
    10741 accagccgtt atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc
    10801 ggttgcgaat aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga
    10861 tgccgaggcg ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct
    10921 gctgtttcag ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt
    10981 caatgaccgc cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg
    11041 tgtgtaatcg agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt
    11101 ggcatagcgg ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc
    11161 gacagcgtta atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc
    11221 tgtgtcgccg ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag
    11281 ttcaactagc gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac
    11341 ctggtaaagc tgttcgctgg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc
    11401 aatctccaga cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg
    11461 tcgcgcaatt gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg
    11521 agaaatcgcc catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg
    11581 gctggacgcc agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca
    11641 acctaatggc gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc
    11701 aacgggttta ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt
    11761 tttgttaaag gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg
    11821 atcgatcttg ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg
    11881 tcggtgaatt cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga
    11941 aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc
    12001 atccccatcg catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc
    12061 atttaacttc cccttgaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt
    12121 tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc
    12181 agctttatct ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc
    12241 cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat
    12301 atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg
    12361 ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta
    12421 actgtgtggc atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca
    12481 ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc
    12541 aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga
    12601 ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg
    12661 aacgcgcgca cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct
    12721 ttaagaaaga tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc
    12781 tggcggcaac cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct
    12841 ataaagcgat taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa
    12901 acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga
    12961 taaactccgg cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg
    13021 ttatcaacaa acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat
    13081 cgatggctga cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa
    13141 ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt
    13201 tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat
    13261 agcactgcca cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc
    13321 ttccctatcg ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt
    13381 ttttgcgatc attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa
    13441 agcaaacgac aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca
    13501 cagcgcggcg gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg
    13561 gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca
    13621 tttactcatg ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat
    13681 caccatccac tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag
    13741 gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat
    13801 atttcacggg tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg
    13861 gagctggggc aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg
    13921 tacaccaacc ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg
    13981 gtcgccatcg gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga
    14041 ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta
    14101 tcgactaagg atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg
    14161 gagctgaaaa atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg
    14221 accgatcccc gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa
    14281 gtgattgccg attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa
    14341 aaacatgaaa acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt
    14401 ctgttctgcc catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg
    14461 caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat
    14521 gacaccggca gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg
    14581 cgaacgcatt attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc
    14641 ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag
    14701 taaaattcat cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga
    14761 aacggttgcg gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg
    14821 ccagcgtgag caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc
    14881 cagcgccatt cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac
    14941 gcatcaaaag gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat
    15001 cgtggtgcag ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga
    15061 agcgcaggcg ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac
    15121 cgattaccag acccggacgg cttattggga attagtccgc gatcactttg caatattgaa
    15181 agtcggtcac gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga
    15241 gcaggaactt atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat
    15301 gctcgacgaa ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact
    15361 ggatattcgt tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa
    15421 aaatagcgtt gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat
    15481 tagtcagtat cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc
    15541 gcatcagctt attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc
    15601 ggaataagga cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg
    15661 tagcgaagtt ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac
    15721 ctggccacag gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca
    15781 gcacgccatt gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct
    15841 gttaaggcca acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt
    15901 atcgttggtt attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg
    15961 tttatttggc aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac
    16021 tcagttaaag gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta
    16081 acaataaaaa ggactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc
    16141 gacctctacg atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt
    16201 tgaattaatc cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat
    16261 atgtaccact gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc
    16321 ttttgtttct ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg
    16381 atgacctatg ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct
    16441 gccggttgtc atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa
    16501 agcgggtctg catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct
    16561 ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt
    16621 ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt
    16681 ggcgattccg attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt
    16741 ggtaaatgtt gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct
    16801 ggcaaccggt tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta
    16861 taagctcggc gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc
    16921 tattccgcac ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat
    16981 cgagaaaatc ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt
    17041 tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct
    17101 cgcgggttac gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct
    17161 gctgatgcca cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc
    17221 ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc
    17281 attactgctg gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat
    17341 tttaattgct gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat
    17401 cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat
    17461 ctcgggtgtc atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca
    17521 cacccaaccg gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga
    17581 tcagggcggt tctcccatta cctg
//
LOCUS       DAJTPG010000082        17388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTPG010000082 DAJTPG010000000
VERSION     DAJTPG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(44..775)
                     /gene="rlmB"
                     /locus_tag="OO230_002871"
     CDS             complement(44..775)
                     /gene="rlmB"
                     /locus_tag="OO230_002871"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5692556.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(955..3396)
                     /gene="rnr"
                     /locus_tag="OO230_002872"
     CDS             complement(955..3396)
                     /gene="rnr"
                     /locus_tag="OO230_002872"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5692557.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3435..3860)
                     /gene="nsrR"
                     /locus_tag="OO230_002873"
     CDS             complement(3435..3860)
                     /gene="nsrR"
                     /locus_tag="OO230_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5692558.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4065..5363)
                     /gene="purA"
                     /locus_tag="OO230_002874"
     CDS             complement(4065..5363)
                     /gene="purA"
                     /locus_tag="OO230_002874"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5692559.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5467..5664)
                     /locus_tag="OO230_002875"
     CDS             complement(5467..5664)
                     /locus_tag="OO230_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5692560.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5746..6750)
                     /gene="hflC"
                     /locus_tag="OO230_002876"
     CDS             complement(5746..6750)
                     /gene="hflC"
                     /locus_tag="OO230_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5692561.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6753..8012)
                     /gene="hflK"
                     /locus_tag="OO230_002877"
     CDS             complement(6753..8012)
                     /gene="hflK"
                     /locus_tag="OO230_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5692562.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8098..9378)
                     /gene="hflX"
                     /locus_tag="OO230_002878"
     CDS             complement(8098..9378)
                     /gene="hflX"
                     /locus_tag="OO230_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5692563.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9454..9762)
                     /gene="hfq"
                     /locus_tag="OO230_002879"
     CDS             complement(9454..9762)
                     /gene="hfq"
                     /locus_tag="OO230_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5692564.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9848..10798)
                     /gene="miaA"
                     /locus_tag="OO230_002880"
     CDS             complement(9848..10798)
                     /gene="miaA"
                     /locus_tag="OO230_002880"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5692565.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10791..12638)
                     /gene="mutL"
                     /locus_tag="OO230_002881"
     CDS             complement(10791..12638)
                     /gene="mutL"
                     /locus_tag="OO230_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5692566.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12648..13985)
                     /gene="amiB"
                     /locus_tag="OO230_002882"
     CDS             complement(12648..13985)
                     /gene="amiB"
                     /locus_tag="OO230_002882"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5692567.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14004..14465)
                     /gene="tsaE"
                     /locus_tag="OO230_002883"
     CDS             complement(14004..14465)
                     /gene="tsaE"
                     /locus_tag="OO230_002883"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5692568.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14437..15984)
                     /gene="nnr"
                     /locus_tag="OO230_002884"
     CDS             complement(14437..15984)
                     /gene="nnr"
                     /locus_tag="OO230_002884"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5692569.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15983..17122
                     /gene="queG"
                     /locus_tag="OO230_002885"
     CDS             15983..17122
                     /gene="queG"
                     /locus_tag="OO230_002885"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5692570.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17105..17158)
                     /locus_tag="OO230_002886"
     CDS             complement(17105..17158)
                     /locus_tag="OO230_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692571.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3945 a         4958 c         4367 g         4118 t
ORIGIN      
        1 aaaaaccatc caaatctgga tggcttttca taattcagag gaactagctg cgctgacgaa
       61 ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt cagggaagaa acacttcctg
      121 ccattgggat gctgatcaac tcatcgcaat gttcacgggt caggcgacgc ataccttcac
      181 cttccgcgcc catcaccagc gccaggcgtc cggtcatttt gctctgatag agtgtatgat
      241 ccgcctcgcc tgccgtaccg acgatccaga tattctcttc ctgcaacata cgcatggtgc
      301 gcgcaaggtt agtcacccga atcagcggaa cgctttctgc cgcgccgcag gctacttttt
      361 tcgccgtagc gttgagctgt gcggagcgat ctttcggcac aatcaccgca tgaacgcctg
      421 cggcgtccgc actacgcagg cacgcgccga ggttgtgcgg atcggttacg ccgtcgagga
      481 tcagcaggaa cggttgatcg agcgaagcga tcagatccgg cagatcgttt tcctgatact
      541 gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac ggcaccgtcg cttttctcgt
      601 cgagatattg gcggtttgcc aactggataa ccacgccctg agactcaagg gcgtgaatca
      661 gcggtaacag acgtttatct tcacggcctt ttaaaataaa gacttcctga aaacgttcag
      721 gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta aatcatttcg ctcattaatg
      781 tactcgttgt ttacgttttg cctgatgcac tacgtttatc cggcctacat gatctctgca
      841 atatattgaa tttgcgtgat tatgtaggcc ggataaggcg ttcacgccgc atccggcatg
      901 aacaaagcgc attttgtcag caatctaacc ctcttctttt aaagagggta ttgatcactc
      961 tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcggctattt tctgcgtttt
     1021 cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc gctttcggct tcgttttttt
     1081 ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt ttaccgacct gacaacgctt
     1141 gccgcctttt ttgcctgcat cgcctttttt cgctttctcg cgcgccgttt taccgacgtt
     1201 gcgcggtgcg cgttcactgg agatcagact aaagtcgatt ttgcgctcgt ccatattaac
     1261 cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa gtctggccgc tggattcccc
     1321 catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg aagagacatg
     1381 gaccagacca tcaatgaaca agtcgtccag acggacgaag aagccaaagc cagtgacgct
     1441 ggaaattacg cctttaaaga cgttacctac ctggtcgagc atgaagtcac acttcagcca
     1501 gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc gccatcgaac agtgctgacc
     1561 cagttgcaac atctcttcca tcgaataatg gtagccgccg gtttcagtgg tgttgccctg
     1621 atgcccctgc tctttcgcca gcagatattt aatggcgcgg tgcagcgtca ggtcaggata
     1681 acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccagaccga agtgaccacg
     1741 gttttctgga tcgtaaatcg cctgtttcat cgagcgtagc agcatggttt gcagcatttc
     1801 tgcatcagga cggtcggcaa ccgactccag cagttccgcg tagtcacgcg gttccggctt
     1861 gttaccaccc ggcagctcca gccccagctc cgccagcact gaacggaaag aggtaatcgc
     1921 ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttcgctt tctcaacgaa
     1981 acgcgccgcc gagatattcg ccagaatcat gcactcttca attaatttgt gcgcgtcgtt
     2041 acgctgggtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc
     2101 ttcgctctca aatgagatcc caccgcgttc ttcacgggct ttatccagca ctttatagag
     2161 gttatgcaac tcttcgagat gcttaaccag cggggcgtac tgctcgcgca gatcctgatc
     2221 gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc
     2281 ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa accgtcatct cgcacaccat
     2341 acacaggcgg tctacctgcg ggttgagcga acacaggccg ttagagagca cttccggcag
     2401 catcgggata acctgcgaag ggaagtacac cgacgtgcca cggttacgcg cttctctgtc
     2461 cagcggcgtt ggcggacgca catagtagct gacgtcggca atcgcgaccc ataaacgcca
     2521 gccgccgccg cgttttttct cgcagtaaac tgcatcgtca aagtcacggg cgtcttcgcc
     2581 atcaatggtg accagcggta aatcgcgcaa atcgacacgg cccgcttttg cttcttccgg
     2641 cacttcttct ttcagccccg caacctgttg ctcaacagcc tgcggccaga tatacggaat
     2701 ttcatgggta cgcagagcga tatcaaccgc catgccggtg cccatattgt cgcccagcac
     2761 ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga cgctgagtca gttcgactac
     2821 gaccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga tatcgaagct
     2881 cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac
     2941 aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac gacctttacg
     3001 gtcagcaccc agcggctgcg ccagcacctg atcgccatga atgcaggttt tcatctgctc
     3061 gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt agccatcacg
     3121 gtgaccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg
     3181 acgagtgaag accagttgac catcgcgctc catcgcgcgc aggcggcgac gcaggccttc
     3241 aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc tggccggttt
     3301 ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat tcgcgtattt
     3361 ttcagcttcg cgttcctgga aaggatcttg tgacatctcg gttcctccgt tgtcatctct
     3421 gatgaagatt ttcgtcactc caccagcaat aatttataaa gcggttgatt ctcttcaacc
     3481 aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc cttagaaagc
     3541 gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca attcaccagc
     3601 gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc cggtttaccc
     3661 aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg actaagttga
     3721 ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac ttcagaaata
     3781 ctggtcatcc gcccttctgg caatgacgcc atgtagatca gcgcacgtaa tccgtaatca
     3841 gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa tacatttata
     3901 ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa
     3961 agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag caatgtatca
     4021 cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc gtcgaacggg
     4081 tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc gatcggcaca
     4141 ccggtcagct cttcaatacg cttgatgtag ttcagcgccg cctgcggcag gccgctacga
     4201 tctttcacgc cgaaagtgga ttcagaccag cccggcatgg tttcgtaaat cggctctaca
     4261 cctttccagt cgtcagctgc cagcggagtg gtagtcactt cgcgaccatc cggcatacgg
     4321 taagccacgc agagtttaac ctctttcagg ccatccagaa cgtccagttt agtcaggcag
     4381 aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc cagccagccg
     4441 gtacgacgac gacgacccgt agttgcaccg aattcgttac cctgcttgca gaggaactcg
     4501 ccagtttcat caaacagttc ggccgggaaa ggacctgcac ctacacgagt tgagtaagct
     4561 ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc ggtcgccacg
     4621 ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc gatatccagc
     4681 agcgtaccct gcgcaccttc gaacatgacg aaatcgccac gctgacgcgc ctggtcgagc
     4741 aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat cgtatcatcc
     4801 agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg gaagttgtga
     4861 tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca
     4921 cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg accggtggtg
     4981 ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagagcaac gtgataatca
     5041 aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat gccacggtct
     5101 tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc gttaccgatg
     5161 atgctggtta cattctcgcg gagaatacct gatggaataa gatggagaac ggttttttca
     5221 ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac aacatattta
     5281 gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca ttgggtgccc
     5341 agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa tggattctac
     5401 catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga tcagtctgtt
     5461 ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg caaccacaag
     5521 tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag agatcatctt
     5581 cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa ccagggcaag
     5641 cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac caccgacatc
     5701 cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac gcgttgcgga
     5761 agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca tcacgtcctg
     5821 attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt agaagtccgg
     5881 atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt caccacgcat
     5941 aatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt cggcagtcgc
     6001 gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg
     6061 ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt tgatctgctt
     6121 gatacgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt tgatgaccgg
     6181 aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc
     6241 tgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac
     6301 ttcgagggtc agacgaccac gggaatcggt gacgatatct ttcacgtcca ggcgaccaat
     6361 ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt gcgaaatgtc
     6421 gccaccaccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt tgatgtaaga
     6481 gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt tgtccatggt
     6541 ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc
     6601 aggctcataa accaaaggtt tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt
     6661 aataccgcgc tcaccttctt tgacgacaaa gacagacatg taaagcacta ccagcacgat
     6721 gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg gtagtcgtta
     6781 cgctgcgcgt tggcgcggcg ttggtccata atatcgccct gactggtgga cgacgtgttg
     6841 cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt gctggcaccg
     6901 ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa cggcagaacc
     6961 atcaggttgc cacctttatc gttaaccagc actttgcggg tgttacccaa cactttttcc
     7021 atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata ttccggcaga
     7081 agtttagcaa agcgcgccac ttcaccctga gcttccagga tggtctgggc cttgtacgca
     7141 cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac ttcgttggaa
     7201 tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca
     7261 aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc cagcagcgtg
     7321 atacccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc gctacgaatc
     7381 acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac tccacgcagg
     7441 gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata cagatatttt
     7501 tccggattgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt ctcgtccgac
     7561 gtcagcatca caccagaagc ggccagttca cgcacggctt ccacgttcac cggtttgact
     7621 tcgtcgataa acgtcggttt ccagttcaga cccggctcaa ccagatggct gaatttacca
     7681 aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact ggccgcccag
     7741 ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg cgggcgcggg
     7801 ccttgtgatg aactgccacc gccagatccg gtgcctttac cgccgcccag accaccgagc
     7861 tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc gcgaccgcct
     7921 ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct
     7981 tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg ttatgcggtg
     8041 atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc tacgccgtta
     8101 gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt caacgatcgg
     8161 catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt ctattgcctg
     8221 aagctgataa aaacgactcc tcagacgccc ttcctgcggt ggcagacgca atgtatgctg
     8281 cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta tccccgctcc
     8341 ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat caatacgcgg
     8401 ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga tctcgtgagc
     8461 gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac gcacatccgc
     8521 cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa
     8581 tgccgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg caagtacggt
     8641 ttcaccgaca tctgcaacgt caatacgccg caacgtcggg tcgagggtgg caaacaactg
     8701 gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt taccggcgtt
     8761 ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt gccgcccctg
     8821 ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc gattacgcaa
     8881 caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccta tcccgccttt
     8941 ctgtctttca aggtgggtcc agccacgcac caggcgcgta gccagatggc gcagctgcgc
     9001 cagctcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa tatctaaaat
     9061 aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac gctcttgcgc
     9121 cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc atcgctttga cagcttccgc
     9181 aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt tacggctacc
     9241 ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa actcctggag
     9301 gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc
     9361 atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa cgcaggatcg
     9421 ctggctcccc gtgtaaaaaa acagcccgaa accttattcg gtttcttcgc tgtcctgttg
     9481 cgcggaagta ttctgcgcgc tgctaccatg atggtagtta ctgctggtac cgccaccggc
     9541 gttgttactg tgatgagaaa ccgggcgaga tgggacaaca gtagaaatcg cgtgcttgta
     9601 aaccatctgg ctgaccgtgt ttttcaacag gatcacgaac tgatcaaaag actcgatttg
     9661 cccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt cccgacgcag
     9721 tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt ttccttatat
     9781 gcttatttgt actttgaacc tttcgattct gaaaaaattg cgcacgatac gtctcaattg
     9841 tacacattca gcctgcgata gcaccaacaa cctgtaatac ttcgtcacgc gcctgttctg
     9901 gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt atctgccgct
     9961 tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat gagatttcgc
    10021 cttcaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc aagtccgtat
    10081 gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa gccaacatct
    10141 gatgaaaacg ctgctcaatg cgttgatgga gcagttcacg gctcgccggg gcgatggcga
    10201 actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc gttaaagttt
    10261 tacccgaaat gaaaaaaact tccagtgccc gggaaagcct ttgtggatca tttggatgaa
    10321 tccttgctgc cgcaaccgga tctacctcct gaagttgacg atgcaatgac tcccaacctt
    10381 gctctgccgc ctgttgctca attctggccc gcacttccgg gtctgccgac ggtagcggcg
    10441 acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaac agtgggatcc
    10501 gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa tcagcagccg
    10561 agtaagcctg cgacggatcg cgaatatcca gcaatcggtg cggcgcggcg agtaactctt
    10621 cagcgttcgg cttcgccgtc ccgatatcca tccctttgta aataaggaca gaatcaacgc
    10681 ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt ttaccggagg
    10741 ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata tcactcatct
    10801 ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc cggcggcgtt
    10861 ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc ctgtgccatt
    10921 gaccactgcg catgttcgct catcagattt cgtgcaatcc actgcgcaat attgccaggt
    10981 tcgaatacgg actgcttcgc caggtagcct atcagttcag gaatcaagat ttgtaaattt
    11041 tgttggcgta agggtaaagg cacggccctg atggtcacat gctgtgcatc tgactggaaa
    11101 tcaataccca attccgctag ggcagactgc gctttttcta atgccgattt ttcttcgcca
    11161 gaaactttta gccgcaacgg aatcagcagc ggctgggcgc aaacgggcgc ttcacccggc
    11221 gtcaattgtg cctgacgcag ccaacgttct gccactggca aggataaaag tgaaatgttg
    11281 ccatcgcgct ccagcaacgc acagtcggaa tggacgatag tcagtacccg accaaaactc
    11341 tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg cttttggttt ttgcatcggc
    11401 gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta gcctggctgc
    11461 gcattcggcc agggggcagc cggacgactg cctgaggcgg gcgcaggaga gtagcgcgga
    11521 gctaccggct cacgagctgc cggttctgca aagtgattgc gccccgccgc cacgcggttt
    11581 tccgggatgg cacgcggtgc gggttgtggt tcatcgtcca gcggtagcgg cgtttccagt
    11641 tgctgttgta gcacgctcag cacgccctga tagataaaat catgcaccag acgcgactga
    11701 tggaaacgca cttcgtgttt ggcggggtgt acgttgacgt ccacctgatg cgggtcgatc
    11761 tccagataca acacaaatgc cggttgctga tcggccccca gtttgtcttc gcaggcctgg
    11821 cggatcgcat gattgatcag gcgatcgcgc atcatgcgac cattcacgta gcaatactga
    11881 atttccgcca gtgcgggcgt agtgtgattt ggatcggcca cccagccgcg cagggtgaga
    11941 tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc gcaaatcgcg
    12001 cctaagcgcc gttctttttg cccgccttcc ggcactgcgc ggtactgacg cacaatttta
    12061 ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca gcgcaatgcg gcggatgatt
    12121 tcatcaatat ggctaaattc ggttttctcg gtgcgcagga atttgcgccg cgccggggtg
    12181 ttgtagaaca gatccagcac ctccagcgtc gttcccaccg gatgcgctgc cggtttaacc
    12241 gtcacgtcca tgtcgcgtcc ttcggcatag gcctgccagg cttcctgctg ttctgcggtg
    12301 cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc accgcgaaag
    12361 cccaggctga taatggcttc gagatcgtcc agagaggcga ttttactggt ggcatgacga
    12421 gccagcgcca gcgccagctc atcttttttg ataccgcagc cgttatcacg aatgcggata
    12481 agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc atcgaggctg
    12541 ttttccacta gttctttgac taccgacgca ggtcgctcga ccacctcacc tgcggcaatc
    12601 tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta gtttggcagc
    12661 gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg ttgcaccctg cggcgcagat
    12721 tgcatcggat gcgcaaggaa gtaattgcgc aggcctttgt aaatggcttc tgccagctgt
    12781 tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttgctgat aaaaccggtt
    12841 tcgaccagta ctgatgggat atccggcgag cgcagaacgc caaggctggc gtgttctggt
    12901 cgacgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt cgctacatca
    12961 taccctaccc gctgggaatg accgaactgt aaatccagca ccgcctggct taaatagggg
    13021 tcagactgac tgttcgccag cacatcaccc gccccaccca gcagctccga cagtttctcg
    13081 tgctgctcca gccagctggc catttcactg ttggcgcgac ggttagagag cacccatacg
    13141 gaagcgccag tcgcactacg gttcggtgcg gcatcagcgt gaatcgacac gaggaaattg
    13201 gcgttttgct tatgtgccac atcactgcgc cccatcaccg agataaagta atccccgtca
    13261 cgggttaaaa cgcctttaaa catcggatcg tcattgagca aagtacgcaa tttacgcgcg
    13321 atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc agggtcctga
    13381 ccgccgtgtc cggcatcaat agcgatgata attttatcgc cagtgttagc cgtcgcgcgc
    13441 gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt actttccgtt
    13501 ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgcagg cgtttcaacg
    13561 cgtttcgcaa ccacgggcgg cggaggaggc ggtggcggca catcggcgtt aatcgtaaag
    13621 acgacagtgt aattgctgcc attctgccgc ttcaccgctt cggttttacc gttttcggta
    13681 agatcgacca ccagccgtag cgtttgtgca tctttaggcg ttccagagcg aatcgccttc
    13741 accagattat tgccgctgaa caacaacggc agtccctgaa tcatgcccgt ttgtttgata
    13801 tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc gccaataaaa
    13861 ctcaacgtta tccgcgcctg ttgattaccg ttagaaacct gaatatcaga gagcgtcgcg
    13921 gcacccaccg gcgtgcacag cagcagcagc gtcgctacca accaatttct gatgcgatac
    13981 atcatcccgc cacctttcaa aggttaaccg gctaaacgcg ccagcaacaa ttcacccgca
    14041 gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg
    14101 acatccgggt caggaagaac acctgtacct tgttgtggcc actccaccag gcagatggca
    14161 tcgttggcaa aataatcgcg gatccccata aactccagct cctcgggatc ggcaaggcgg
    14221 tacaaatcaa agtgatagac cattaagttg tcgagcgtat agggttcgac cagcgtataa
    14281 gtggggcttt tgacattacc ctgatgaccc agagcctgta aaaagccccg gctaaaggtg
    14341 gttttacctg cgcctaaatc gccatacaga tagattacgg ttgcgccatc gcaggctttc
    14401 gctacccgct cgcccaggtc taatgttgcc tgctcatcag ggagcggaat tactcgattc
    14461 atcatggttt ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc
    14521 ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg ccgcaccgtg
    14581 cgcgacacag cctgcacagg ctgcatcata cggcgacagt ttttgcccaa gcaatgcgcc
    14641 aataatacca gagagcacat cgcccatgcc gccgctcgcc atgcctgcat ttccggcatc
    14701 aataatgcct aaagcgtcag gatgggcggc gaccacggtt ccggcacctt tcagcaccgc
    14761 tacgccgcca taacgttgta ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc
    14821 agcgacggaa cagcctaaca accgtgcggc ctcgctagga tgcggcgtga tcacgcgatt
    14881 gtgacgctta tcgggattga ttgccagcag gttcaatgca tcggcatccc acaacatcgg
    14941 tttgcgaaaa ttctcaactt tttgcagggc ttttttcccc cactcttgct ggcccagacc
    15001 gggaccaatc accaccacat cggcccattc caggctttcg gtaagagagt ccatagtcag
    15061 ttcatgcacc atcaattctg gtcgtgcagt cagcagcggc gcaatgttct cactgcgggt
    15121 cagtactcgg actaaaccag caccagcacg tagcgccgct tcccccgtca tacgaatagc
    15181 ccccgccgtg ccgtgatcgc caccgataat caccagccgc ccgtgatcgc ctttatgcga
    15241 agtcgggcga cgcggtttca gccagtgaga aagttgttct gccgaaaacc gctgaatttt
    15301 cgtctcctga cctgccagcc agctatccag ccccagtgag tcaaaatgca gttgtcctgt
    15361 aacatcccgc gcttttccag tgagcaagcc tagtttcagc gcaataaaag tgatggtgtg
    15421 atcggcgttg atcactgcgc ctggcgtagc gccggtttca gccagcaggc cggaagggat
    15481 atcaaccgcc acaatcggcg caggatggga attagcgtgg tcgattaact ggctaatgga
    15541 ttcgcggggc gcttgctgca agccggtacc gagcagcgca tcaacaatca gatctaccga
    15601 ttcgggccag acaatattcg aagcatggat ctcgccaccc gcgtttaacc atgcttcgcg
    15661 tgccagcgcg gcctcttccg gcaacggttt gtcgctctcc tgggccaaca acgtgacctc
    15721 aatgccgacc gctttggcca gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg
    15781 accgcacagc accagccagt ggcgggcgtc aggatacgcc gaacgacaca cctggaatgc
    15841 ggcctcgcca gcgcgaagca tcagctcata gagtgtgagc cccagcacat ctgccgcctc
    15901 gcgttctccg cggcggatat cgtcggcgta ccagacggtg tgtggtatac ttacggggtt
    15961 tttcttcatt gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa
    16021 aattaaacag tgggggctgg aactgggctt tcagcaggta ggtattaccg ataccgatct
    16081 cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa caataccacg gcgaaatgga
    16141 ctggatggca cgtcacggta tgctgcgcgc tcgcccccat gagttattac ccggtacgct
    16201 gcgtgtaatc agcgtgcgga tgaattacct tcctgctaac gccgcatttg ccagcacgct
    16261 gaaaaacccc aaactcggct atgttagccg ttatgcgctg ggccgtgact atcacaaact
    16321 tctgcgcaac cgactcaaaa agctgggcga gatgattcag caacattgtg tttcgctgaa
    16381 ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg aaaaagctgg
    16441 gctcggctgg acaggtaagc actcacttat cctcaatcgc gaggccggtt cgttcttctt
    16501 tttaggcgaa ttgctggtcg atattccgct gcccgtggat caaccagtcg aggaaggatg
    16561 cggcaaatgc gtggcctgta tgacgatttg cccgaccggt gccatcgtcg agccatatac
    16621 cgtcgatgct cgccgctgta tctcttatct caccatcgaa cttgaagggg cgctcccgga
    16681 agagttgcga ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc agcttatctg
    16741 cccgtggaat cgctattcgc aactcactac agaagacgat ttcagcccgc gtaagccgct
    16801 acacgcaccg gaactcattg agttatttgc ctggagcgaa gagaagtttt taaaagtcac
    16861 ggaaggatcg gcgattcgcc gtataggtca cctgcgctgg ctgcgtaata tcgccgtggc
    16921 attaggcaat gccccttggg atgaaacgat tttgacagcg ctggaaagtc gtaaaggtga
    16981 gcacccactt ctcgatgagc acatagcgtg ggcgattgcg cagcaaatag agagacgaaa
    17041 tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg tgattgaaaa
    17101 agggttaccg cgtgacgcct gattcatcca cagcttgtga ataaaaataa aaacgcattg
    17161 caattcaaga cgaataaaat cgtcaagtga tcgactcgac aaaacggaat cttgattttt
    17221 aacttattta tcaataggtt aaaaaaatac cattcaccgt gcgaagtttc tttgaaatta
    17281 agattacagc ctgaggctgt ggataactct gtttacaaga gtttgtcaga gacaacaaaa
    17341 accagcaata acgcatcgcg tcgctgtaga tattttattg agagaaga
//
LOCUS       DAJTPG010000083        17385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTPG010000083 DAJTPG010000000
VERSION     DAJTPG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            44..487
                     /locus_tag="OO230_002887"
     CDS             44..487
                     /locus_tag="OO230_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5692572.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            723..1541
                     /gene="ybhA"
                     /locus_tag="OO230_002888"
     CDS             723..1541
                     /gene="ybhA"
                     /locus_tag="OO230_002888"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5692573.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1542..2600)
                     /gene="modC"
                     /locus_tag="OO230_002889"
     CDS             complement(1542..2600)
                     /gene="modC"
                     /locus_tag="OO230_002889"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5692574.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2603..3292)
                     /gene="modB"
                     /locus_tag="OO230_002890"
     CDS             complement(2603..3292)
                     /gene="modB"
                     /locus_tag="OO230_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5692575.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3292..4065)
                     /gene="modA"
                     /locus_tag="OO230_002891"
     CDS             complement(3292..4065)
                     /gene="modA"
                     /locus_tag="OO230_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5692576.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4232..4381)
                     /gene="acrZ"
                     /locus_tag="OO230_002892"
     CDS             complement(4232..4381)
                     /gene="acrZ"
                     /locus_tag="OO230_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5692577.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4510..5298
                     /gene="modE"
                     /locus_tag="OO230_002893"
     CDS             4510..5298
                     /gene="modE"
                     /locus_tag="OO230_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5692578.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5366..6838
                     /gene="modF"
                     /locus_tag="OO230_002894"
     CDS             5366..6838
                     /gene="modF"
                     /locus_tag="OO230_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5692579.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7099..8115
                     /gene="galE"
                     /locus_tag="OO230_002895"
     CDS             7099..8115
                     /gene="galE"
                     /locus_tag="OO230_002895"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5692580.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8125..9171
                     /gene="galT"
                     /locus_tag="OO230_002896"
     CDS             8125..9171
                     /gene="galT"
                     /locus_tag="OO230_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5692581.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9175..10323
                     /gene="galK"
                     /locus_tag="OO230_002897"
     CDS             9175..10323
                     /gene="galK"
                     /locus_tag="OO230_002897"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5692582.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10317..11357
                     /gene="galM"
                     /locus_tag="OO230_002898"
     CDS             10317..11357
                     /gene="galM"
                     /locus_tag="OO230_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5692583.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11560..12312
                     /gene="gpmA"
                     /locus_tag="OO230_002899"
     CDS             11560..12312
                     /gene="gpmA"
                     /locus_tag="OO230_002899"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5692584.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12479..13531)
                     /gene="aroG"
                     /locus_tag="OO230_002900"
     CDS             complement(12479..13531)
                     /gene="aroG"
                     /locus_tag="OO230_002900"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5692585.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLSAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13847..14227
                     /locus_tag="OO230_002901"
     CDS             13847..14227
                     /locus_tag="OO230_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5692586.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14341..15282
                     /gene="zitB"
                     /locus_tag="OO230_002902"
     CDS             14341..15282
                     /gene="zitB"
                     /locus_tag="OO230_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5692587.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15279..15998)
                     /gene="pnuC"
                     /locus_tag="OO230_002903"
     CDS             complement(15279..15998)
                     /gene="pnuC"
                     /locus_tag="OO230_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5692588.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16036..17079)
                     /gene="nadA"
                     /locus_tag="OO230_002904"
     CDS             complement(16036..17079)
                     /gene="nadA"
                     /locus_tag="OO230_002904"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5692589.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4430 a         4539 c         4320 g         4096 t
ORIGIN      
        1 caggaaaata gcagacgtcc agaacgaatg gttcggcctt tgcatggatc ttgcactggt
       61 aacaggacag cgcaaaggag atctggcggc tatgagatgg gaggatataa gagatagcag
      121 gctatacgtg gaacaacaaa aaacaggggc aaaaattcgc atttctctcc ccaccacgat
      181 ttccagattg aatttaacac ttgccgatgt actggataac ttgaaaaaaa taaacggtaa
      241 aaacgaaaag ttactcggag ggaagacagc caggacaatt gcagcacagt tcaggattgc
      301 ccgagacaca tccggattga aatgggaagg tgacccaccg ccatttcatg aaatccgaag
      361 tttatccggt cgcctgcact cagcagaaaa aggcagtgat ttcactcagg cgctccttgg
      421 acatcgttca tccagcatga cggataaata ccgtgacgga cgagggaggg aatggaaaga
      481 catttagaat taatttcgaa tgatttcgaa atgccacttg taattaattg attataaaga
      541 attaattcct aaagagcatt tgcttcatga atgctccttt gcatttagca actgactgta
      601 acagctaaaa ttagtcgctt ttggcggtga atgctgaatt aaaacgcaga attttgtccg
      661 atagtggtat cggtgtacca ttcgcgcaga cgaaaattct taaccttaat ctggaatacg
      721 ccatgaccac acgcgtgatt gctctcgact tagacggcac cttattgacc ccgaaaaaga
      781 ccttgcttcc atcatcgata gaagccctgg cccgcgctcg cgaagcaggc tatcaattaa
      841 tcatcgtcac aggtcgccat cacgtcgcta ttcatccttt ttatcaggcg ctggcgctgg
      901 atacacctgc tatttgctgt aatggcacct atttgtatga ttatcatgca aaaaccgtgc
      961 tggaagcgga cccaatgccc gttaataaag ccctgcaact cattgagatg ctgaatgaac
     1021 accacattca cggtctgatg tatgtcgatg atgcaatggt ctatgagcac ccgaccgggc
     1081 atgtcattcg cacgtctaac tgggcgcaaa ccctgccgcc ggaacagcgt ccgactttca
     1141 cacaagtcgc ttctctggct gaaacgacgc aacaagttaa cgccgtatgg aagttcgccc
     1201 tgacgcacga tgacctgccg caattgcagc attttggtaa gcatgtcgaa catgaactgg
     1261 gactggagtg tgaatggtcc tggcacgatc aggttgatat tgcacacggc ggcaacagca
     1321 aaggtaaacg tttgacgaaa tgggttgagg cgcaaggttg gtcgatggaa aacgtcgtgg
     1381 cattcggcga taactttaat gatatcagta tgctggaagc cgctggtaca ggcgtggcga
     1441 tgggcaacgc cgatgacgcg gtaaaagcgc gcgccaacat tgtgattggt gataacacca
     1501 ccgacagcat tgcccagttc atttatagcc acctgattta atcaggcggt tatcgacaca
     1561 cttttaattt gcgcgtacag ccacaggcca ggtttgatcg ccagttcatc cctggcccac
     1621 gggctgatac gcgcccacag cgttttaccg ccgacttcca gctccacttc cacctggccg
     1681 ttgtcgtcat aactattaac aacttttgcc cgcaatacgt tacgaatgct ggtttgctgc
     1741 ggcggttgta aaaccagaga aacatcggaa gcctgaatgc ggatacgtag cgcagcttgc
     1801 agcggttcgc ccagcttatt gacccacaaa tgctgatcgc ccagcgccag cgcggtcatc
     1861 gcgtaatgcg gatgatgctc aagcaccgtc actttcagaa tgctactttg ttgctctttc
     1921 ggcagccacg gattcatcac gctactgccc cacacttcct ccagcgcgcc aaaggctttc
     1981 acctgaccgt tttccagtac catcactctg tctgccagat ggaggatctc atccagcgaa
     2041 tggctgacat acaacatcgg aatgttgatt tcccgtgtca gccgttgcag ataaggcaac
     2101 agttcgcgtt tacgcggaat atccagtgac gccagcggtt catccagcaa cagcaattcc
     2161 ggtgctgtca gcaaagcccg accaatcgcc acgcgctgtt tttcgcctcc ggacaggctg
     2221 cctggtaaac ggtcaagcaa cggttcaatg cctaaaagcg ccaccagctt atcgaactga
     2281 tcgaccatac ttttcgacat gccgtagcgc agattgccac gcactttgta atgcgggaac
     2341 aaccgcgcat cctgaaaaac atagccaaca cgacgctttt ccggcgtcag gcagatacct
     2401 ttttcggcat catttagtac ccgcccattg aggacaatcc gccctttttg cgggcgcgtc
     2461 agtccactga tggcgttaat cagcgaagtt tttccggcac cggagacgcc aaagatagca
     2521 gtgatgccat tggcgggcag cgtttcatta atggtcaggc aatggttgcc caacgtctgg
     2581 gaaaaattca gctccagcat gattagcgcc ccgcccgttc acggctgatt ctggccagcc
     2641 attctgaaat caacagggag atcatcgcca gcgcaataga aataatgcac agtctcgccg
     2701 cgccactttc cccgccgggg gtctggatca gggtatacat ggcagaagga atggttcgcg
     2761 tttcgctagg aatgttcgac acaaaggtga tggttgcacc aaactcaccg agagaacgag
     2821 caaaagccag taccgtacca acaataattc ccggtaaggt cagcggtaac gtgatagtaa
     2881 agaaaacgcg ccagcgcccg gcacctagtg ttcttgcggc ctgttccagt ttgacatcaa
     2941 ccccttccag cgccagacga attgcccgca ccatcagcgg aaacgacatg acggcggcag
     3001 cgagaaccgc gccgcgccag ctaaaggcga aggtaatacc aaaccagtca tacagacgtt
     3061 caccgataaa tccgcgccgt cccatcgaaa ctaataataa gtaaccgacg accacgggcg
     3121 gtaacaccag cggtagatgc agtacgctgt cgagcagagc tttgcccgga aacgtgcaac
     3181 gcaccagtaa ccaggcaaaa aagatcccaa acggcaggct aaacagcaca gccagggaag
     3241 aaactttcag gcttaataaa actgcctgcc attctggatc ggtcagtatc attacttggt
     3301 tgtaaatccg taacgtttaa agatttccgc tgcctgcggt cccttcagat aatcataaaa
     3361 tgctttcact gtcgcattgt tatgcccttc cacaacagca accggatatt ccactttttt
     3421 atgtgaatct tccgggaagg tggcaaccac ttttacccct ttgctggcaa ctgcgtcaga
     3481 accgtagaca atgcccagag gcgcttcgtt acgttcgacc agcgccagcg ccccacgaac
     3541 atcttccgct ggggccagtt tcggagagag cgtatcccat gcgcccagtt tttgcagtgc
     3601 ttcttttgca taaatgccag cgggaacatg ttccggatcg ccaaccgcca ggcgaccgcc
     3661 attcagcagc gaagtccagt tagttttgct gtcgatggtg aaatctttct gctcgctggc
     3721 tttcggtgct acaacgacca ggctattgcc gagcagtgtc tgacgcgtag cagtatcgat
     3781 cgctttttta tcaaccgcat aatccatcca tttctgatcg gcagaaataa acagatccgc
     3841 aggggcaccc gcttcaatct gacgggcgag agtagatgac gaagcgaaag aagaaaccac
     3901 atccacgcct ttctcttttt tatactgcgt agcaatgtcc tgcattgcgt tagtcagtga
     3961 tgcggcggcg aacaccgtga ttttcccttc atctgccagt gcattgccag caactgcgaa
     4021 agagagtgcc gccccggcaa acaagttcaa ccatttacga gccattggta accccttaat
     4081 gtaacgttat gtaggcgaca atataacgac taactcggaa ttttcccagc agttattgct
     4141 aacctttgtg aggtagataa gaaaaaatat cggcaggaaa agcaggaagt tgagaaaaag
     4201 aaaatgcccg actaagcggg cactcaggga atcaatgatt ttgtccgggc tggtcttttt
     4261 taccaacacc agaaaagatg ttgaatactt caccaagacc gtaaatcaga cccaggatga
     4321 tggccatcac gacaggtacc atgattacgg cgaataccag actttttaat aactctaaca
     4381 tggtcaactc cagatatagt catgagacta ttctaaccgc taagcacaga aaagcactcc
     4441 ccttttgtgc ggtcagcttt gcgtggcgtt cgttttccgt cacaataaga cttttgccag
     4501 gacattgtta tgcaggccga aatccttctc acccttaagc tccaacaaaa attattcgcc
     4561 gacccgcgcc gcatttcgct actaaaacac attgcgcttt ccggttccat tagccaggga
     4621 gcgaaagatg ccggtatcag ctataaaagc gcctgggatg ccattaacga gatgaatcag
     4681 ttaagtgagc atattctggt cgagcgcgca acaggcggta aaggtggcgg aggcgcagta
     4741 ctgacccgct atggtcagcg actgattcag ctctatgact tactggcgca aatccagcaa
     4801 aaagcctttg atgtgttaag tgacgatgac gccctgccgc tgaacagcct gctggccgcg
     4861 atctcacgtt tttcactgca aaccagcgcc cgtaaccagt ggttcggtac catcaccgcc
     4921 cgcgatcatg atgacgttca acagcatgtt gatgtcttac tggctgacgg aaaaacacgc
     4981 ctgaaagtcg caattaccgc acaaagcggc gcacgtctgg ggctggatga aggcaaagaa
     5041 gtgttgatat tgctaaaagc gccgtgggta ggcattactc aggacgaagc ggtcgcacaa
     5101 aacgccgaca accaattacc gggtattatt agtcatattg agcgcggcgc agagcagtgc
     5161 gaagtattaa tggcgctacc cgacgggcaa acactgtgcg ccacagtgcc ggtaaatgaa
     5221 gcgacttctc ttcagcaagg gcagaatgtc acggcctact ttaatgccga cagcgtgatt
     5281 atcgccacgc tgtgctaagc gtgttgacaa tttgttatga aacacgtatc cctgtcagta
     5341 atcgctgcac aaaatgggat ataaaatgtc atcgttgcaa attttgcaag gcacgtttcg
     5401 tcttagcgac acaaaaacgc tgcaattgcc tcagctaacg ttaaacgcgg gtgatagttg
     5461 ggcgtttgtc ggttcgaatg gaagtggaaa gtcggcgctg gcccgcgcgc tggcggggga
     5521 acttccgctt ttgaaaggtg aacggcaaag ccagttttcc cacatcactc gtctctcctt
     5581 cgagcaattg caaaagctcg tcagcgacga atggcaacgg aataacaccg atatgctcgg
     5641 ccctagcgaa gattacaccg gacgcactac ggctgagatt attcaggatg aagtaaagga
     5701 tgcaccgcgt tgcatgcaac tggcgcagca gttcggtatt accgccctcc tcgaccgacg
     5761 ctttaaatac ctttccactg gcgagacgcg aaaaaccctg ctgtgtcagg cgctgatgtc
     5821 ggagcctgac ttgttgattc ttgatgagcc gttcgatggc ctggatgttg cctcacgtca
     5881 gcagctggct gagcgactcg cctcgttaca tcagtccggt attactttgg tactggtgct
     5941 caatcgcttc gataagatcc cggaatttgt ccagtttgct ggcgtgctgg cggattgtac
     6001 gttagcggaa actggcgcta aagaggaact gctccagcaa gcactcgtcg cgcaacttgc
     6061 gcatagtgaa cagcttgaag gtgtgcaact gccggagccg gatgaacctt cagcacgtca
     6121 cgccttaccc gccaacgaat cgcgcattgt gctgaacaat ggcgtggttt cttataatga
     6181 tcgccccatt cttaataacc ttagctggca ggtgaatcca ggcgaacact ggcaaattgt
     6241 cgggccaaat ggcgcgggaa aatcgacgtt attaagcctg attactggcg atcatccgca
     6301 aggttacagc aacgatttga cgcttttcgg ccgacgtcgc ggcagcagcg aaaccatctg
     6361 ggacatcaaa aagcatatcg gttacgtcag cagtagtttg catctggatt accgggtcag
     6421 cactaccgtg cgtaatgtga ttctttctgg ctattttgat tcgattggca tttatcaggt
     6481 cgtttcggat cgccagcaaa aactggtgca gcagtggctg gatattctcg gcattgataa
     6541 acacacggct gacgctccgt tccatagtct ttcctgggga cagcagcgtt tggcgctgat
     6601 cgtccgcgca ctggtgaaac atccgacgtt gcttattctc gatgaaccac tacaggggct
     6661 tgatccgctc aatcgccaac ttatccgccg ttttgttgat gtgctgatta gcgaaggtga
     6721 aacgcaattg ttgtttgttt cgcaccacgc tgaagatgcg cctgcctgta ttacccatcg
     6781 ccttgagttc gtgccggacg gtggactcta tcgctatgtg ctgacaaaaa tatattgagt
     6841 cggtagtgct gaccttgccg gaggcggcct aagcgcactc tccggccaac ggttcgacgc
     6901 atgcaggcat gaaaccgctt cttttttcag ataaaaagcg caatcattca taaaccctct
     6961 gttttataat cacttaatcg cgcataaaaa acggctaaat tcttgtgtaa acgattccac
     7021 taatttattc catgtcacac ttttcgcatc tttgttatgc tatggttatt tcataccata
     7081 agcctaatgg agcgaattat gagagttctg gttaccggtg gtagcggtta cattggaagt
     7141 catacctgcg tgcaattact gcaaaacggt catgatgtca tcattcttga taacctctgt
     7201 aacagtaagc gcagcgtact gcctgttatc gagcgtttag gcggcaaaca tccaacgttt
     7261 gttgaaggcg atattcgtaa cgaagcgttg atgaccgaga tcctgcacga tcacgctatc
     7321 gacaccgtga tccacttcgc cgggctgaaa gccgtcgccg aatcggtaca aaaaccgctg
     7381 gaatattacg acaacaatgt caacggcact ctgcgcctga ttagcgccat gcgcgccgct
     7441 aacgtcaaaa actttatttt tagctcctcc gccaccgttt atggcgatca gccccaaatt
     7501 ccatacgttg aaagcttccc gaccggcaca ccgcaaagcc cttatggcaa aagcaaattg
     7561 atggtggaac agatcctcac cgacctgcaa aaagcccagc cggactggag cattgccctg
     7621 ctgcgctact tcaacccggt tggcgcgcat ccgtcgggcg atatgggcga agatccgcaa
     7681 ggcattccga ataacctgat gccatacatc gcccaagttg ctgtaggccg tcgcgactcg
     7741 ctggcgattt ttggtaacga ttatccgacc gaagatggta ctggcgtacg cgattacatc
     7801 cacgtaatgg atctggcgga cggtcacgtc gtggcgatgg aaaaactggc gaacaagcca
     7861 ggcgtacaca tctacaacct cggtgctggc gtaggcagca gcgtgctgga cgtggttaat
     7921 gccttcagca aagcctgcgg caaaccggtt aactatcatt ttgcaccgcg tcgcgaaggc
     7981 gaccttccgg cctactgggc ggacgccagc aaagccgacc gtgaactgaa ctggcgcgta
     8041 acgcgcacac tcgatgaaat ggcgcaggac acctggcact ggcagtcacg ccatccacag
     8101 ggatatcccg attaaggaac gaccatgacg caatttaatc ccgttgatca tccacatcgc
     8161 cgctacaacc cgctcaccgg gcaatggatt ctggtttcac cgcaccgcgc taagcgcccc
     8221 tggcaggggg cgcaggaaac gccagccaaa caggtgttac ctgcgcacga tccagattgc
     8281 ttcctctgcg caggtaatgt gcgggtgaca ggcgataaaa accccgatta caccgggact
     8341 tacgttttca ctaatgactt tgcggccttg atgtctgaca cgccagatgc gccagaaagc
     8401 aacgatcctt tgatgcgttg ccagagcgcg cgcggtacca gccgggtgat ctgcttttca
     8461 ccggatcaca gtaaaacgtt gccagaactg agcgttgcgg cattgacgga aatcgtcaaa
     8521 acctggcagg agcaaaccgc agagctggga aaaacgtacc cgtgggtgca ggtctttgaa
     8581 aacaaaggtg cggcgatggg ctgctctaac ccgcatccgc acggacagat ttgggcaaat
     8641 agcttcctgc ctaacgaagc tgagtgcgaa gaccgcctgc aaaaagaata ttttgccgaa
     8701 cagaaatcac caatgctggt ggattatgtt cagcgcgagc tggcagacgg tagccgtacc
     8761 gttgtcgaaa ccgaacactg gttagccgtt gtgccttact gggctgcctg gccgttcgaa
     8821 acgctactgc tgcccaaagc ccacgttttg cggatcaccg atttgaccga cgcccagcgc
     8881 agcgatttgg cgctggcgtt gaaaaagctg accagtcgtt atgacaacct cttccagtgc
     8941 tccttcccct actctatggg ctggcacggc gcaccattta atggcgaaga gaatcaacac
     9001 tggcagctgc acgcgcactt ttatccgcct ctgctgcgct ccgccaccgt acgtaaattt
     9061 atggttggtt atgaaatgct ggcagagacc cagcgagacc tgaccgcaga acaggcagca
     9121 gagcgtttgc gcgcagtcag cgatatccat tttcgcgaat ccggagtgta agaaatgagt
     9181 ctgaaagaaa aaacacaatc tctgtttgcc aacgcatttg gctaccctgc cactcacacc
     9241 attcaggcgc ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa cgacggtttc
     9301 gttctgccct gcgcgattga ttatcaaacc gtgatcagct gtgcaccacg cgatgaccgt
     9361 aaagttcgcg tgatggcagc cgattatgaa aatcagctcg acgagttttc cctcaatgcg
     9421 cccattgtcg cgcatgaaaa ctatcaatgg gcgaactacg ttcgtggcgt ggtgaaacat
     9481 ctgcaactgc gtaacaacag cttcggcggt gtggacatgg tgatcagcgg caatgtgccg
     9541 cagggtgccg ggttaagttc ttccgcttca ctggaagtcg cggtcggaac cgtattgcag
     9601 cagctttatc atctgccgct ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa
     9661 aaccagtttg ttggctgtaa ctgcgggatc atggatcagc taatttccgc actcggcaag
     9721 aaagatcatg ccttgctgat tgactgccgc tcactgggga ctaaagcagt ttccatgccg
     9781 aaaggtgtgg ctgtcgtcat catcaacagt aacttcaaac gtaccctggt tggcagcgaa
     9841 tacaacaccc gtcgtgaaca gtgcgaaacc ggtgcgcgtt tcttccagca gccagctctg
     9901 cgcgatgtca ccattgaaga gttcaacgct gttgcacatg agctggaccc aatcgtggcg
     9961 aaacgcgtgc gtcatatcct gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg
    10021 gagcaaggcg acctgaaacg tatgggcgag ttgatggccg agtctcatgc ctctatgcgc
    10081 gatgatttcg aaatcaccgt gccgcaaatt gacactctgg tagaaatcgt caaagctgtg
    10141 attggcgaca aaggtggcgt acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg
    10201 ctgatcccgg aagagctggt gcctgccgta cagcaagctg tcgctgaaca atatgaagca
    10261 aaaacaggta ttaaagagac tttttacgtt tgtaaaccat cacaaggagc aggacagtgc
    10321 tgaacgaaac tcccgcactg gcacccgatg gtcagccgta ccgactgtta actttgcgta
    10381 acaacgcagg gatggtagtc acgctgatgg actggggtgc gactttactt tccgcccgta
    10441 ttccgctttc cgatggcagc gtccgcgagg cgctgctcgg ctgtgccagt ccggaatgct
    10501 atcaggatca ggctgcgttt ctgggggcct ctattggtcg ttatgccaac cgcatcgcca
    10561 atagtcgcta tacctttgac ggtgaaaccg tgacgctttc gccaagccag ggcgttaacc
    10621 agctgcacgg cggcccggaa gggttcgaca aacgtcgctg gcagattgtg aaccagaacg
    10681 atcgtcaggt gctgtttgcc ctgagttcag atgatggcga tcagggcttc ccgggtaatc
    10741 tcggcgcgac ggtgcaatat cgtctgaccg acgataaccg tatctccatt acttatcgcg
    10801 ccacagttga taaaccttgc ccggtgaata tgactaatca cgtctatttc aatcttgacg
    10861 gcgagcagtc tgacgtgcgc aatcacaagt tgcagattct ggcggacgaa tatcttccgg
    10921 ttgatgaagg cggcattccg cacgacggct tgaaatctgt cgccggaacg tcttttgatt
    10981 tccgcagcgc caaaatcatc gccagtgagt ttcttgccga cgacgatcag cgcaaagtga
    11041 aaggttacga tcacgcattc ttgttacagg ccaaaggcga tggcaagaaa gtggcggcgc
    11101 atgtctggtc agcagatgaa aaattgcagc tgaaggtcta caccaccgct ccggctcttc
    11161 aattctactc cggcaacttc ctcggcggca caccgtcgcg gggaaccgaa ccttacgccg
    11221 actggcaagg gctggcgttg gaaagcgagt ttctgccgga cagcccgaac caccctgaat
    11281 ggccgcaacc ggactgcttc ctgcgtcctg gcgaagagta ttccagcctg acggaatatc
    11341 agtttattgc tgagtaatgt taagccctcg tcatgagggc tttatcttca tattgttcaa
    11401 atcaccagca aacaccgaca tatttgcaac tcaatattca caacaacctt acactgcgcc
    11461 actattttcg ctatggttat gcgtaagcat tgctgttgct tcgtcacggc aatataatga
    11521 gaattattat cattaaaaga tgatttgagg agtaagcata tggctgtaac taagctggtt
    11581 ctggttcgtc atggcgaaag tcagtggaac aaagaaaacc gtttcaccgg ttggtacgac
    11641 gtggatctgt ctgagaaagg cgtaagcgaa gcaaaagcag caggtaagct gctgaaagag
    11701 gaaggttaca gctttgactt tgcttacact tctgtgctga aacgcgctat ccataccctg
    11761 tggaatgtgc tggacgaact ggatcaggca tggctgcccg ttgagaaatc ctggaaactg
    11821 aacgaacgtc actacggtgc gttgcagggt ctgaacaaag cggaaactgc tgaaaagtat
    11881 ggcgacgagc aggtgaaaca gtggcgtcgt ggttttgcag tgactccgcc ggaactgact
    11941 aaagatgatg agcgttatcc gggtcacgat ccgcgttacg cgaaactgag cgagaaagaa
    12001 ttgccgctga cggaaagcct ggcgctgacc attgaccgcg tgatccctta ctggaatgaa
    12061 accattctgc cgcgtatgaa gagcggtgag cgcgtaatca tcgccgcaca cggtaactcc
    12121 ctgcgtgcgc tggtgaaata tcttgataac atgagcgaag aagagattct tgagctgaat
    12181 atcccgactg gcgtgccgct ggtgtatgag ttcgacgaga atttcaaacc gctgaaacgc
    12241 tattatctgg gtaatgctga cgagatcgcg gcgaaagcag cagccgttgc gaaccagggt
    12301 aaagcgaagt aaacgtcatt cgtttaaaaa atgagaaagc cgactgcaag tgagtcggct
    12361 tttttgtttg tcgttgctaa caatggagca cattgcctga tgcgacgctg cgcgtcttat
    12421 caggcctgtg gtgattcatc ggatacgcca ctctgacggc gcatccgaca attaaacctt
    12481 acccgcgacg cgcttttact gcattcacca gttgacgtaa cagagcatcg gtatcttccc
    12541 agccaatgca ggcatcggtg atgctcttac cgtaggccag cggctccccg ctatcgaggc
    12601 tctgattgcc ttccaccaga tggctttcca ccatcacgcc aataatggcc ttttcgccac
    12661 cggcaatctg ctggcaaacg tcagcacaaa catccatctg ctttttgaat tgtttggacg
    12721 aattagcatg gctgaaatcg atcatcacct gagctgacag acctgctttg ttcagccctt
    12781 ctttcacttc agcaacgtgc ttcgcgctgt agttaggctc tttaccgccg cgcagaatga
    12841 tatggcaatc gccgttacca ctggtattca caatcgccga atgcccccat ttagttacgg
    12901 acaggaagca gtgcggcgca ccggcggcat taatggcatc gatagccact ttaatcgtac
    12961 cgtctgtgcc atttttaaaa cctaccggac aagaaagacc agacgccagt tcgcggtgca
    13021 cctgcgattc cgtggtacgt gcaccaattg cgccccagct catcaggtca gcgagatact
    13081 gaggagtgat catatcgaga aactcacccg ccgctggcag accgctgtcg ttaatatcaa
    13141 gcagcaattt acgggctata cgcagaccgt cgttgatctg gaagctgtta tccatatgcg
    13201 gatcgttaat caaccctttc cagcccaccg tggtacgcgg cttttcaaaa tagacgcgca
    13261 ttacgatttc cagctcatct ttcagctctt cacgcagcgc cagcaagcga gtggcatact
    13321 ctttagccgc gacaggatca tgaattgagc acgggccaat caccaccaac aggcgatcat
    13381 cattaccttt caggatctta tggatcgctt ttcgggcatg ggcaaccgta ttcgcggcat
    13441 tttcagttgc ggggaatttt tccagcaatg cgacaggagg aagtaactct ttgatttctt
    13501 tgatgcgtaa atcgtcgttc tgataattca tgtctgttcc agtgttgcca tacttatctt
    13561 agtgaatgca atacttccaa tctatatctt ccgtcagaat gtgtaaacgg ggttttacac
    13621 tatgaacgga ttaatcctgg aattagcaag aaaaacgcca gattgtcgcg aaaaacgaga
    13681 tctctcctac aatttctaac tgtaactcct ttgtttgtta attatttcaa gattctctgc
    13741 tgcgtttcat aaactggctg aaatcttaaa ccaatgcctt atattcacct gcaaatgcac
    13801 tgttggaaga ggttatccga cataacgacc ataacaggag catcctatga aaatgacaaa
    13861 actggccaca ttatttctga ctgccactct aagccttgcc agcggtgccg cactggctgc
    13921 cgatagcgga gcgcaaacta ataacggcca ggcaaacgcc gcagctgatg cgggccaggt
    13981 agcccctgac gcccgtgaaa atgtcgcgcc aaataacgtc gacaataacg gggtaaatac
    14041 cggttctggc ggcacaatgc tgcattcgga tggttcttca atgaacaatg acggaatgac
    14101 caaagatgaa gagcacaaga acaccatgtg caaagatggt cgatgtccgg atatcaataa
    14161 aaaggtacaa accggtgatg gcatcaataa tgatgtcgat accaaaaccg acggtaccac
    14221 gcagtaaccc tgcaaaatag ccaataatgg gagagcggag tctctccctt ttaatttttc
    14281 acttctaaaa gcagtatgat gtcataaagt taagtgagaa taattaaggg atgactcgtc
    14341 atggcgcact cacactcaca cacgtcctca cacctgccag aagataataa tgctcgtcgc
    14401 ttgttgtatg ctttcggcgt gactgccggg tttatgctgg tagaagtcgt tggtggtttt
    14461 ctttctggtt ctctggcatt gctggccgat gcgggtcata tgttgaccga tactgccgcc
    14521 ctgcttttcg ccctactcgc cgtgcaattt tcacgtcgtc ctcccaccat tcgccacact
    14581 ttcggttggc taagactgac cacgctcgcg gcgtttgtga atgccatcgc cctcgtggta
    14641 attaccattt tgattgtctg ggaggcgata gaacgcttcc gcacgccgcg tccggtcgag
    14701 ggaggcatga tgatggcaat tgccgtggct gggttgctgg caaatatact ttctttctgg
    14761 ttacttcatc acggcagcga agagaaaaac ctcaacgtac gagcagcggc actgcatgtg
    14821 ctcggggacc tgctgggttc ggttggggct attattgccg cgttgattat tatctggact
    14881 ggctggacac ctgctgaccc cattctctcg atactggtgt cgcttctggt tctgcgtagc
    14941 gcgtggcgac tgttgaaaga tagcgtgaat gaattacttg aaggtgcacc ggtatcgctg
    15001 gatatcgctg aactgaagcg tcgtatgtgc cgggaaatcc cggaagtccg caatgtgcac
    15061 catgtacatg tatggatggt aggcgagaag ccggtgatga cgttgcatgt gcaggtgatc
    15121 ccgccacacg atcacgatgc cttgttggat cagatccaac actacctgat ggatcactat
    15181 cagattgagc acaccaccat tcagatggaa tatcaacctt gtcataggcc agactgccac
    15241 ctgaatgaag gggtgtctgg ccattcacat catcaccatt aatgggacag cgcgcgtgag
    15301 cctctttcac gtgcgctgtt gatccacatc cggctgccgt tgagcgcaat aaaggtcagg
    15361 atgatgtact ccagagacat ggcgtaaacg ccctgaagtg caaaaataac gacgctaatc
    15421 acgttaataa tcacccacaa cagccagttt tccacatact tacgcgtcat cagaatcatt
    15481 gccacgatag ataacaccat catgcatgaa tcccagaacg ggaaagcgtc cggttgcagt
    15541 tcaggcatca caacctgtaa tcctaatgct tgcatgatca tgactgccac gcgggtcaaa
    15601 aatgcaaaca ccggattgat aaataccgtc atcagaccaa tcgaaacaac gcaaaccgcc
    15661 aaccagctga gtgccttcgg caatggcaac cagcgaattt tcaactccgc ctcgttctga
    15721 ctggtttgtc gcgaccacgc ataccagccg taaatattcg cggcaaagaa aaacacctgt
    15781 aatagcaggc tggcatacag ctgaatctga aagaaaataa tgccaaacaa ggtaacgttg
    15841 atcaggccaa agaagtagtt gctgatcttc tccagactgg caaggccaat acacagcaac
    15901 ccggcgatcg tgcctaccgc ttcgatccat gagagatcat aaccgcccgc ccctattggt
    15961 atatgtacca ggatattctg cacactaaaa aaatccatct ttcccccaaa gcgtaacgtt
    16021 acgccttatt attcgttatc cacgtagtgt agccgcaaaa tccagcatac gattgagcgg
    16081 caccagcgcc ctctctcgca gcctctcatc gacatgaacc tcgtgattac ttccttcctg
    16141 ttctaatgcc tctgcgatag cctgaaggtc attcatggcc atccacggac aatgcgcgca
    16201 gctgcggcag gttgcgccct cacccgcggt tggtgcttcc agtaactctt tatctggcac
    16261 cgcctgctgc attttgtaga aaataccccg atcggttgcc acaataagcc tctgatgagg
    16321 caatgctttt gcagcagcga tcagttgact ggtggaaccg accgcatccg ccatatcgac
    16381 aatagcttgt ggtgattctg gatgcaccag tatggcagca tccgggtatt cttcttgcaa
    16441 gcgggttaac gcctgagtct taaatgcatc atgcacaatg caggcaccct gccagcataa
    16501 aatgtctcca cccgtctgtt tttgcacgta acgccccaga tgtttgtcgg gtgcccagat
    16561 gattttttca cccaaactat caagatgatc aataagttcg acggcaatgc ttgaagttac
    16621 cacccaatct gcgcgcgctt ttaccgcagc agaagtgttg gcgtagacga cgacagtacg
    16681 atcgggatgg gcatcgtaaa atgcgttaaa ttcttcaaca gggcagccga gatccagtga
    16741 acactcaacc tgaagtgtcg gcatcagaat tgttttttcc ggactgagaa ttttggcggt
    16801 ttctcccata aatctcaccc cagcgactaa caaagtagaa gcgggatgct ttgcaccgaa
    16861 gcgcgccatt tccagagaat cagaaataca gccaccggtt tcttctgcca gttgttgaat
    16921 ttcgggatcg gtatagtagt gggcaaccat caccgcatta cgttctttta gcagacgttt
    16981 tatcttctcg cggtaatacg ctttttcatc aatgcttaac ggcgtcggct tcggggggaa
    17041 aggataaatc gccgtgtctg gatcaaacat tacgctcatc ttaccatctc gttttacagg
    17101 cttaacgtta aaaccgacat tagcgtaaca ttcgctgtta tcggtgtaat ttgttttata
    17161 tgctaaacaa gatagtcgaa tggtggggaa aagtcactcg aattacgcct aattccaccg
    17221 acgtagagtt gcttgcacag taagctctga cgatgtcatc aggtttacaa agtgaaattg
    17281 aggatgcctg aatagcaaaa aagcgccttt agggcgcttt tttgcattgg tgggtcgtgc
    17341 aggattcgaa cctgcgacca attgattaaa agtcaactgc tctac
//
LOCUS       DAJTPG010000084        17279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTPG010000084 DAJTPG010000000
VERSION     DAJTPG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(1..613)
                     /gene="chbR"
                     /locus_tag="OO230_002905"
                     /pseudo
     CDS             complement(1..613)
                     /gene="chbR"
                     /locus_tag="OO230_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(621..971)
                     /gene="chbA"
                     /locus_tag="OO230_002906"
     CDS             complement(621..971)
                     /gene="chbA"
                     /locus_tag="OO230_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5692590.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1030..2378)
                     /gene="chbC"
                     /locus_tag="OO230_002907"
                     /pseudo
     CDS             complement(1030..2378)
                     /gene="chbC"
                     /locus_tag="OO230_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2463..2783)
                     /gene="chbB"
                     /locus_tag="OO230_002908"
     CDS             complement(2463..2783)
                     /gene="chbB"
                     /locus_tag="OO230_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5692591.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3082..3420)
                     /gene="osmE"
                     /locus_tag="OO230_002909"
     CDS             complement(3082..3420)
                     /gene="osmE"
                     /locus_tag="OO230_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5692592.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3622..4449
                     /gene="nadE"
                     /locus_tag="OO230_002910"
     CDS             3622..4449
                     /gene="nadE"
                     /locus_tag="OO230_002910"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5692593.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4679..5566
                     /gene="cho"
                     /locus_tag="OO230_002911"
     CDS             4679..5566
                     /gene="cho"
                     /locus_tag="OO230_002911"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5692594.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5526..6101)
                     /gene="ves"
                     /locus_tag="OO230_002912"
     CDS             complement(5526..6101)
                     /gene="ves"
                     /locus_tag="OO230_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5692595.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6304..6789)
                     /gene="spy"
                     /locus_tag="OO230_002913"
     CDS             complement(6304..6789)
                     /gene="spy"
                     /locus_tag="OO230_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5692596.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7119..8087)
                     /gene="astE"
                     /locus_tag="OO230_002914"
     CDS             complement(7119..8087)
                     /gene="astE"
                     /locus_tag="OO230_002914"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5692597.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8080..9413)
                     /gene="astB"
                     /locus_tag="OO230_002915"
                     /pseudo
     CDS             complement(8080..9413)
                     /gene="astB"
                     /locus_tag="OO230_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9410..10888)
                     /gene="astD"
                     /locus_tag="OO230_002916"
     CDS             complement(9410..10888)
                     /gene="astD"
                     /locus_tag="OO230_002916"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5692598.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10885..11919)
                     /gene="astA"
                     /locus_tag="OO230_002917"
     CDS             complement(10885..11919)
                     /gene="astA"
                     /locus_tag="OO230_002917"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5692599.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11916..13136)
                     /gene="astC"
                     /locus_tag="OO230_002918"
                     /pseudo
     CDS             complement(11916..13136)
                     /gene="astC"
                     /locus_tag="OO230_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13582..14388
                     /gene="xthA"
                     /locus_tag="OO230_002919"
     CDS             13582..14388
                     /gene="xthA"
                     /locus_tag="OO230_002919"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5692600.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14555..15265
                     /locus_tag="OO230_002920"
     CDS             14555..15265
                     /locus_tag="OO230_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5692601.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15270..15947
                     /gene="ydjY"
                     /locus_tag="OO230_002921"
     CDS             15270..15947
                     /gene="ydjY"
                     /locus_tag="OO230_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5692602.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15962..16669
                     /locus_tag="OO230_002922"
     CDS             15962..16669
                     /locus_tag="OO230_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5692603.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16669..17217
                     /locus_tag="OO230_002923"
     CDS             16669..17217
                     /locus_tag="OO230_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5692604.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
BASE COUNT         4269 a         4599 c         4208 g         4203 t
ORIGIN      
        1 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
       61 ctttatcatg catcttttct accgtacttt tcagccacta cggtacatca tcaatcacct
      121 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
      181 cttccagccc tgtttcgcgg aaatttaatg aagaaatcac tgtttccaca taggtgagaa
      241 aagcgttatt ggtccggtat acctgcgaag caacaaagca ataaggcaac aacggcaggt
      301 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatgcgcgtg gcaccaaact
      361 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
      421 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
      481 aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt gttatagata aacacatgga
      541 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
      601 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
      661 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
      721 ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
      781 gcttcattca atgccatccg tgactgatcc atcatggctt tcgctgcggc aaaatcgccc
      841 tgcttcgcct gtttcaatgc ggcatacgcc aggctacgcg cttgtccaga gttgatgata
      901 agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg aatattatcg
      961 agatccatca tacatcgttc ctctctcttc ttaccggcaa gattacccgt accggcatcg
     1021 attaaaattt caggggttag cgatatcttc ttcgctctct tctttatcaa tcgcattttg
     1081 tgctttgtta gccaccacaa caaagggcag ataaattaac gttgcgatgc caaggttgaa
     1141 gagtgcgacc agcaatgcgg cgacgctacc gttggtgtta aagaaggctc ccagaccggt
     1201 tggcatggtc cacggtgcaa tattggtcac cggaggaatg atgcccatgt agtacgctgc
     1261 gagggtgatt gccgccagaa tcggttgtac cagtacaaac gggataaaca tcaccgggtt
     1321 catgataatt ggcagaccaa acagaatcgg ttcattaatc tggaagatgc cggacggcag
     1381 cgccagtttt gccacctgac gataatcagc acggcgagag gcgataaaga tagccaggat
     1441 caggcctaaa gtcgcaccac tgcccccaag gaaaataaag gagtccagca tcggcttggc
     1501 ccagatatgg aaggtcttac cggctgccag cgccgcttcg acggaaccat attgctgata
     1561 ggtcgcgata ttttccagcg cccacggcgt cataatgccg ttgtccagtg cggtcagcgc
     1621 cagcgcgcca tgaataccga agaaccagag cagtggaaca aagatcacat aggcccagcc
     1681 caccacgcta cccaacgatg ccagtggggt tgagatggta tccataatga tctgatggaa
     1741 gttggtgccc caggtattca acgcccaggc aataatcccc atcacggaaa gaataataaa
     1801 gccgggaatt aatgccgaga aagaacgcga tactgaagca ggtacgctgt cgggcagttt
     1861 aatgacccaa ttgcggcgga caataaaggt aaacatttct gccaccacca ggccaataat
     1921 aatcccggag atgatattcg ccccacctaa ccagtttgcg ccaaccgcat aggcctcacc
     1981 gacactatat ggggtgacgg tcataaatgc tgcaacggat aacaacccag ccgccagcgc
     2041 atcgacttta cgctcttctg ccagcgccat gccaataaag aacggtgcca ttaaagacat
     2101 tattcctaat gttccgttat atacgttgcc gccaatacct ttcagaccat taagtgtttc
     2161 aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa acgacccctc cccaaagctt
     2221 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     2281 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     2341 aggagtacct tttcaagcga tgcaataaca ttactcatag aaaaataccc ttaaaaaccg
     2401 caatttaaat attacggtat tgatttatga aataactctt tgacgggaaa atttaaaata
     2461 aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac gcctaaacca
     2521 tcgactttgc cataaagcag cgagtcaatt acttcaaccg gtttgttggg taacaaacgc
     2581 tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc ggcattctga
     2641 cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac ttcatatttt
     2701 tctgcctgtg cgcgcatttt tgataccagt aaagagatag acatgcccgc agaacaaaac
     2761 agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg tcagccagac
     2821 actccgcaag ccttgacctg cttccatgct ctgggtaact tgcgaaacca acatgacttt
     2881 ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct ttgaccttat
     2941 aaattgtttc tccttgacct gtggttatga ccctcttcac atttcgggca aataattcgc
     3001 gaagataatt aagaactcag gccaaaaaaa accggcgcaa tggccggttt ccgttgttac
     3061 tcaagctttc agacgaattg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
     3121 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
     3181 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccgcgag catggatcat
     3241 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
     3301 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagctgtaca
     3361 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
     3421 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     3481 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttaaccacca
     3541 ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg aagttttgtc
     3601 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     3661 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     3721 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     3781 acgcttgccg gaaagctgtg ccagatggcg attaatgagc tgcgccagga aactcgcaac
     3841 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggctga cgaacaagat
     3901 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     3961 gcggtattgg ctagcgagca ggcattgcgg gaagcaggca ttgaactgag cgattttgtc
     4021 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     4081 agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt cttcactaaa
     4141 tatggtgacg gcggtacgga tattaatccg ctgtatcgtc tcaacaaacg ccagggtaaa
     4201 cagttactga cggcattagg ttgcccggaa cacctttata agaaagcccc aactgccgat
     4261 cttgaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     4321 atcgacgact atctggaagg gaaaaaccta cctgaacagg tcgccagaac aatagagaac
     4381 tggtatctga aaaccgaaca taaacgccgt ccgccaatta acgttttcga tgatttctgg
     4441 aaaaagtaat aatttgcacc tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     4501 tgcggcgtga acgccttatt ctacctacaa aactatgcaa attcaataca ttgcaggagt
     4561 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc aacccgcctc
     4621 ttttttaatc gcctcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     4681 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     4741 cgaacattta cattcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     4801 tggcgaaagt gacaccatgc cgctctatat cggcaaaagc gttaatatcc gcagtcgggt
     4861 gctttctcat ttacgcaccc cggatgaagc cgccatgcta cggcaatccc ggcgaattag
     4921 ctggatctgc accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     4981 acaacagccg ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg ccctgcaatt
     5041 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     5101 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga gcatcgctga
     5161 tgaacaaaaa ctttgttatg gcctgttggg actggaaccg ttaagtcgcg gtcgtgcatg
     5221 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     5281 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcga gtggtgtgtt ggccttggca
     5341 aggtgcggtg gcactgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     5401 ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg acaacgttaa ttcggacacc
     5461 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     5521 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     5581 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacacgccc
     5641 tggtcggtag tgagcagttt gtcgcccagt tgccacgccc cgttaatcac aaacaccacc
     5701 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     5761 acatccaggc gcgtcataat attgaaatcc atcgacatct gccctgccgt cagtttcgct
     5821 tttaccacct ggtccgccgt aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     5881 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     5941 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     6001 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcgtttcgc
     6061 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     6121 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     6181 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     6241 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     6301 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     6361 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     6421 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     6481 cgcttcagct tttgctttat cgaaggtatc gctggcaatg atgtcatgca ttgcgcggcg
     6541 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgtggatctg
     6601 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     6661 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggtag tggtgtctgc
     6721 ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca gtgcagttaa
     6781 tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt aagtagcgtg
     6841 ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc aacggtaaaa
     6901 caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa aatttgatgg
     6961 agagtgatga gagaatatta caacacgatg attttgcaga gattatgaag aactataccg
     7021 gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt tataagaaag
     7081 caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt ttcgagcatt
     7141 aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata ctctacatca
     7201 tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt ctcaaacggc
     7261 ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg gcgagtaatt
     7321 tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact ctcaccagat
     7381 agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt ttgcccaaag
     7441 ggcaacgctt tgccaagttc caacgtacag gccagcgcgc caaaatgtct gacgctgaaa
     7501 tgggtaaacg taccaccagg ctcctgatgg aacaccagcg cctccagccc cgccgcaccc
     7561 agccacgtca gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac accgaactgc
     7621 ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccaacgcac agattctttg
     7681 ccctggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca ggtttctccg
     7741 ctttcagcaa atagctgcca acgaccgcca aacattcggt tcatatcgct atggcaataa
     7801 cgtttccctt gcttcagcgc aggaggattc ccgaggatca ccagcaaccg ccaacgtaac
     7861 gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca gcatttccac aggtgccgtc
     7921 tcattaccgt ggatccccgc agaaatcacc agcgcgcctt gcggtggcgt taatggcgtc
     7981 agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc tcgctcggtg
     8041 ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattgc cccctccctc
     8101 gcgctggaac ggataaaccg aaccgagatt cagtaattgg ctcaatacat ccagtgcttc
     8161 ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca gcagtaagac gatcgcggta
     8221 gtaacgatcc acccagtcat tgagcgcatt aaacaaggta tcgttcatca tcaccgccgg
     8281 attcaccgcc cggcgttctt cttctgtcaa taccacccgc aaccgcaggc acgccgggcc
     8341 gccgccattc gccatgcttt cacgtaagtc aaagactttt agttcgctaa tcgggttgtc
     8401 agcggcaagg agttcattga gataacccca tactccggcg tgttcccgac actcctgagg
     8461 cagcaccaac atcatggaac catcatcgcg gctcaacagt tggctgttaa acagataggt
     8521 agacaccgca tccgacacgg aaacctgagt tgccggaact tctatcgcca taaaaccgtt
     8581 cacccgcgtg ccagtaactg tgactggcga gcgaacgcct gttggtggca aaacagcacc
     8641 tggcggttac tcacggcaat cacgtcatta tgaaaaacgc tctggtcgat aacgtccggg
     8701 ttttgctggg cgaaaatcac ctgttgggga ttcacctgat taagccttgc caccgcctcg
     8761 ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt gccttcttct
     8821 cgcccgtaga caaaaagtag catacccggt tcaccgtaat gaccgccgag acgattgtgg
     8881 tttgccgccc cctcatcacc gagcaacgct acctgcggca acgccgaatg gacgctaaat
     8941 ttctcttcgt ccttaaaaat cgcttttaac agcgattcag tgacgggcgc ttccagcgaa
     9001 cggtgaaatt tattgttcag gttggcaacg gtgagatgca ctttgccatc cagcgtatcg
     9061 gcagatggcg cgatcgttgc cgcattggct acccacattg gcgaagcgga actgacgctg
     9121 gaaagccagt gcggtgcctg gcgtgcaact ttttccagta cctgctcatc gctaccactg
     9181 aatcccaact gacgcagcac cggaataaac ggacgctcgt gcggcggaat cacggcctgg
     9241 gggaatcccg catcggcaag ggctttcatt ttcagtaagc cctgttttgc cgccagtcgc
     9301 gggttagaca cctgaaaacg gtgacgggta gaggcttcat taccaaacga caggcccgcg
     9361 taatgatgcg tcagccctac cagcccgtcg aaattaactt cccaggcgtt catcgcacca
     9421 cctcatcgga aaaatccagc ccggggttaa gcgtggcggg caatgttaac gagtccgact
     9481 ccaggctcgc cataggccat gcgcagtaat ctgcggcata ccaggcgctg gggcgatggt
     9541 taccggaagc accaatgccg ccgaatgggg cggtactggc agcaccagta agcggtttgt
     9601 tccagttaac aatccccgcc cgcgcctcca gcaacagttg atcgaatttt tcccgctcgg
     9661 gggaaaccag accgcaaaag agtccgaagc gagtgttgtt cgccatccga atcgcctcat
     9721 caaaagtatc ataacgccag acgcgcaaaa acggcccgaa cacctcttca tccggcacac
     9781 cgccaacgcc cgtcatttca atgatccctg gtgtcagcaa cgatgtccct gcttgtaata
     9841 agcgcggcgc aagcagggtt cgtccgccca tcgcttccag ttgctgccag gcagtaacca
     9901 cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt tcgtcatccc
     9961 agttgcccgg cgttaatcgc tggctgacgg caaccagact agcaagaaac gcatcgccct
    10021 gcgctccgct tttgagcaat aaacggcggg cgcaggtgca gcgttgcccg gctgtgacaa
    10081 acgccgactg aatggtcaga tggacagccg cgtcgatatc cgccacctca tcgataatta
    10141 gcggattatt accgcccatc tcaagggcga gaattttctc cggctgaccg gagagctggc
    10201 gatgcagctg gtagccggta ttggcgctac cggtaaacag caaaccgtcg agatcctcca
    10261 gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttcagc acgcccggcg
    10321 gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc actccacggt gtcagttcgc
    10381 tgggtttaaa gataatggtg ttacctgcca gcaatgccgg aacgatatgt ccgttcggca
    10441 aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg cgatgtcgca
    10501 ggctcgccgc accgtccggc atttcactac gctgctcgcc ggtacgaacg tgatacgcct
    10561 taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct tcccagcgcg
    10621 gcttacccgt ttctctggca ataatcgcgg ttaattcggc tttattgctt tccagcaatc
    10681 cggcaaagcg ttcgacaacg acctgacgtt cagccaatga gagccgcgcc cagcgcggaa
    10741 acgccgcacg ggctgcccga caagcctgtt cgacctgagc ggcatcggca tcattgcctt
    10801 gccataacac ctcgcccgat accggattac gcttcacacg cgatgcgccc tggcccgtta
    10861 tccagtcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca ggcgcaccag
    10921 acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg ttaaaatcaa
    10981 acgctcggtt gccggatcgg tacgcgccag caccacgcgg aaatggtgat aattttcatt
    11041 ggcgaccagg caggccggaa aatcgccctg cgcaggctgc ccttctgcca cttccaccag
    11101 ccgactttta cggatggcgc gcacgcggtc gatgtcacac tcaagcgttg gcccgccgtc
    11161 gaagatgtcg atatagttgc ggtaacgaaa accttctttc tccagcaccg cgcgggcagg
    11221 cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgggata aaaagtgggt
    11281 atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg tgccgcagag
    11341 aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta ccgaggcttt gccagaacgg
    11401 tgaatagccg tgttcgtcaa tcaccccgcg catttcagca accactttgt cattaaactt
    11461 gtcgcgaaaa gccgccataa acataaagcg cgatttcgat agcaaatagc cgttgccctc
    11521 tttgcgccag tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg tgtgatcgtt
    11581 actaagaaac agcgtcggca atgcgttata gacattcagc tcttttgagg cgtgaaccaa
    11641 cgtgccgacg cgatagttgt accagggatc gttcagccca accgccacct caatagcgca
    11701 aatccccgcc acggtgcctg tctcgctatc ttccagcacg aacacatagc cctgctcgct
    11761 tttgggcagt tcaccttgcc aggttttgat tgccctttcg atacgcgccg aaagcgtggc
    11821 ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg ctggcaagct gcatcagcgc
    11881 cgagacatct gagcgctcaa cgggacggat gaccatcatg atgaacctcg gctaacaaag
    11941 tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc gctgacattg
    12001 agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccatcac gcctgctttc
    12061 gccgcttcca gagagatctg tttcgcttgc ccggcgtaat cggcattcag tacacagcca
    12121 atcaacaaac ctaagccgcg aacttcactg aacaaaccat agcggtgatt aatagtatta
    12181 agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc tggtgtgttg
    12241 atgagctcca gcactttgcc tgctaccgcc gaggccagcg gattaccgcc ataggtggtg
    12301 ccatgagtgc caacggtcat cacgcgggcg cactcttcgg ttgccaacaa cgcaccgacc
    12361 gggaaaccgc cgcccagcgc tttggcggtg gttaacagat cgggcgtcac gccgtagtgc
    12421 atataggcat acagctcacc agtgcgcccg acgccggttt gtacctcatc aaaaatcagc
    12481 aaggcattgt ggcggtcaca caattcacgc agaccttgta aaaacgcgtt gctggctggc
    12541 accacaccgc cttcgcccta gatgggctcg acaatcaccg cacaggtggc gtcgtcaatc
    12601 agcgcgctgg cagagttaat atcgttatat gcagcatgac gaatatccgc cggcagtggc
    12661 gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag cgtgcgacca
    12721 tgaaacgcat ttttgaacgc cacgatgcca ctcttatggc tgccgtagcg gtcgtgagcg
    12781 aatttacgcg ccagttttag cgccgcttcg ttggcttccg caccggagtt acaaaagaag
    12841 acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca gtcgcagtac cggctcgttg
    12901 gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc ttcacgcagt
    12961 tccggatgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc gatatactct
    13021 ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa gggtgccgga
    13081 gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg agacatagcg
    13141 acctctacag taaataattc gttatttgta tgttaacaat aagtaatgtt tgcgctgtaa
    13201 atgtagattg cagggttcgt gccagccagt gataaaagtg cataaacggc ggaggctaac
    13261 tggaaatcaa ggagttataa ccaaaccata tgcatttaaa gtgcatataa agtgaatacg
    13321 tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg ttatgcagaa gtaaaatata
    13381 attctagaat tgtgatcatt gacgaaattt actggaaatt actgcgccat tctgacgcag
    13441 cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca tcattaacaa ccatcgatca
    13501 aatcacttaa caacaggcgg taagcaacgc gaaattctgc taccatccac gcactcttta
    13561 tctgaataaa tggcagcgac tatgaaattt gtctctttta atatcaacgg cctgcgcgcc
    13621 agacctcacc agcttgaagc catcgtcgaa aagcaccaac cggatgtgat tggcctgcag
    13681 gagacaaaag ttcatgacga tatgtttccg ctcgaagagg tggcgaagct cggctacaac
    13741 gtgttttatc acgggcagaa aggccattat ggcgtggcgc tgctgaccaa agagacgccg
    13801 attgccgtgc gtcgcggctt tcccggtgac gacgaagagg cgcagcggcg gattattatg
    13861 gcggaaatcc cctcactgct gggtaatgtc accgtgatca acggttactt cccgcagggt
    13921 gaaagccgcg accatccgat aaaattcccg gcaaaagcgc agttttatca gaatctgcaa
    13981 aactacctgg aaaccgaact caaacgtgat aatccggtac tgattatggg cgatatgaat
    14041 atcagcccta cagatctgga tatcggcatt ggcgaagaaa accgtaagcg ctggctgcgt
    14101 accggtaaat gctctttcct gccggaagag cgcgaatgga tggacaggct gatgagctgg
    14161 ggggtggtcg ataccttccg ccatgctaat ccgcaaacag cagatcgttt ctcatggttt
    14221 gattaccgct caaaaggttt tgacgataac cgtggtctgc gcatcgacct gctgctcgcc
    14281 agccaaccgc tggcagaatg ttgcgtagaa accggcatcg actatgaaat tcgcagcatg
    14341 gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc gccgctaatt tagcagctct
    14401 cctggctcaa actgggtcag gagaattaac cttgagaaaa atcaacaaac tgtcagtaat
    14461 gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt ttttagttgt ctgaccactt
    14521 ctctattatc aagtttgata taggaaactc cacgatgaac gctgagcgta actttctgtt
    14581 tgcctgtctt atttttgcgc tggtcattta cgctatccac gctttcggtt tattcgatct
    14641 gctcaccgat ttaccccact tacagacact cattcgccag agcggattgt tcggttatag
    14701 cctctatatt ctgttattca tcaatgcccc cctctttctg ttaccaggaa gcatactggt
    14761 gatcgctgga ggaatagttt ttggcccgct cttagggaca caactctcat taattgccgc
    14821 aacgctggcc tcctcgtgct cattcctgct ggcgcgctgg ctgggacgtg atttactgct
    14881 gaaatacgtg ggccatagcc ataccttcca ggccattgaa aaaggcattg cgcgtaacgg
    14941 tattgatttt cttattctga cccgcttaat cccgttgttt ccttacaata ttcaaaatta
    15001 cgcttacgga ttcaccacca tcgccttctg gccttatacc cttatttcgg cactcacgac
    15061 cctgcccggt attgttattt ataccgtgat ggcaagcgat ctcgccaatg aaggcattac
    15121 gctgcgcttt attttacaac tctgtctggc gggcctggcg ctgtttattc tcatccagct
    15181 cgcaaaactc tacgcccgac acaaacatgt ggatctgtct gcttcgcgcc gcagcccact
    15241 tactcaccca aaaaatgaag gatagaacga tgtcgcaaca ttattcagtg tcatggaaaa
    15301 aaggactggc tgcactctgc ttactggctg ttgccgggct tagcggctgc gatcaaaaag
    15361 agaatgcggc ggcaaaagtg gaatacgacg gactttcgaa cagccaaccg ctgcgcgtcg
    15421 atgccaataa ccatacggta accatgctgg tgcaaattaa tggtcgtttc ctcaccgacg
    15481 acactcgtca cggtattgtg tttaaagatg gctccaacgg acataaatcg ctgtttatgg
    15541 cttatgcgcc cccgaaagca ttttatgaag ccctgaaaga ggcaggtggt acgccgggcg
    15601 aaaacatgac gatggataat aaagaaacga ctcatgtcac aggcagcaaa ctggatattt
    15661 cggtcaactg gcaaggggcg gcaaaagcgt attccttcga tgaagtgatt gttgacagta
    15721 atggcaagaa actggacatg cgctttggcg gtaatttaac ggcagcagaa gagaagaaaa
    15781 cgggttgcct ggtgtgtctg gatagctgcc cggtcggcat cgtcagcaat gcaacataca
    15841 cttatggtgc ggtggaaaaa cacggcgaag tgaaattcaa aggcaatgcc tcagttctcc
    15901 cggcggataa cacgctggca acggttacct ttaaaatcac cgaataaagc caagataaag
    15961 gatgatgaag atacaatcgc gtaaaatctg gtactaccgt ataaccctca tcatcctgtt
    16021 gttcgctatg ctgctcgcat gggcgctgct tcccggcgtc catgagttta tcaatcgcag
    16081 cgttgcggcg tttgccgccg tggaccaaca gggtatagaa cgctttattc agtcttacgg
    16141 cgcactggcg gcagttgtct cgttcttgtt gatgatttta caggccattg ctgcaccgct
    16201 acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt ggcgcgttct ggggcggctt
    16261 gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc ttttttatcg ccagagtgat
    16321 gggccgcgaa gtggtggaaa aattaaccgg caaaaccgtg cttgacagta tggacggctt
    16381 tttcactcgc tacggcaaac acaccatcct ggtctgtcgg ttattgcctt ttgtcccttt
    16441 cgatccaatc agctatgctg ccggtctgac ttcaatacgt tttcgctcgt tttttatcgc
    16501 caccgggctt ggtcaattac cggcgactat tgtttattcc tgggcaggca gcatgttaac
    16561 aggcggtact ttctggtttg tcaccgaact gtttattctg tttgccctga ccgtggtgat
    16621 ttttatggcg aagaaaatat ggcttgagcg ccagaagagg aatgactgat gggtttaccg
    16681 ccgcttagca aaattccttt cattttacgt ccgcaggcgt ggctgcatcg tcgccattac
    16741 ggcgaggtgc taagcccgat tcgctggtgg gggcggatcc cgtttatctt ttatttggtg
    16801 tcaatgtttg ttgggtggct ggagcgcaaa cgctcaccgc tcgatccggt agtgcgatcg
    16861 cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt tttgcgtgga tatcaccagt
    16921 atgaaagtcg ccgagcgtac cggcagcacc gataaactgc tggcagtggc tgactggcgg
    16981 caaagcccgc tctttagcga tgaagaacgg ctggcgctgg agtacgccga agccgcaagc
    17041 gtcacaccgc caacagtcga tgatgccctg cgtacccgac tggctgcgca ttttgacgct
    17101 caggcgctca ccgaactgac ggcattgatc ggcctgcaaa acctgtcggc tcgttttaat
    17161 tctgccatgg acattcccgc tcaggggctg tgccgtattc cggaaaaacg ttcttaagga
    17221 gggaagatgc gccattgtgg gtggttgctg ggattgttat cgctgttttc tttggcaac
//
LOCUS       DAJTPG010000085        17000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTPG010000085 DAJTPG010000000
VERSION     DAJTPG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..419
                     /locus_tag="OO230_002924"
     CDS             <1..419
                     /locus_tag="OO230_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5692605.1"
                     /translation="EKKLLQKSIEIDGFTQPIVVTHTDKNAMEIVDGFHRHEIGKGSS
                     SLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIVRELSQLGWDDNKI
                     GKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(420..1580)
                     /gene="ybdL"
                     /locus_tag="OO230_002925"
     CDS             complement(420..1580)
                     /gene="ybdL"
                     /locus_tag="OO230_002925"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5692606.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1689..2777
                     /gene="hcxA"
                     /locus_tag="OO230_002926"
     CDS             1689..2777
                     /gene="hcxA"
                     /locus_tag="OO230_002926"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5692607.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2787..2984)
                     /locus_tag="OO230_002927"
     CDS             complement(2787..2984)
                     /locus_tag="OO230_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5692608.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3166..5271)
                     /gene="cstA"
                     /locus_tag="OO230_002928"
     CDS             complement(3166..5271)
                     /gene="cstA"
                     /locus_tag="OO230_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5692609.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5452..5865)
                     /gene="entH"
                     /locus_tag="OO230_002929"
     CDS             complement(5452..5865)
                     /gene="entH"
                     /locus_tag="OO230_002929"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5692610.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5868..6614)
                     /gene="entA"
                     /locus_tag="OO230_002930"
     CDS             complement(5868..6614)
                     /gene="entA"
                     /locus_tag="OO230_002930"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5692611.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6614..7471)
                     /gene="entB"
                     /locus_tag="OO230_002931"
     CDS             complement(6614..7471)
                     /gene="entB"
                     /locus_tag="OO230_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5692612.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7485..9095)
                     /gene="entE"
                     /locus_tag="OO230_002932"
     CDS             complement(7485..9095)
                     /gene="entE"
                     /locus_tag="OO230_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5692613.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9105..10280)
                     /gene="entC"
                     /locus_tag="OO230_002933"
                     /pseudo
     CDS             complement(9105..10280)
                     /gene="entC"
                     /locus_tag="OO230_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10572..11528
                     /gene="fepB"
                     /locus_tag="OO230_002934"
     CDS             10572..11528
                     /gene="fepB"
                     /locus_tag="OO230_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5692614.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11532..12764)
                     /gene="entS"
                     /locus_tag="OO230_002935"
     CDS             complement(11532..12764)
                     /gene="entS"
                     /locus_tag="OO230_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5692615.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12875..13879
                     /gene="fepD"
                     /locus_tag="OO230_002936"
     CDS             12875..13879
                     /gene="fepD"
                     /locus_tag="OO230_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5692616.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13876..14868
                     /gene="fepG"
                     /locus_tag="OO230_002937"
     CDS             13876..14868
                     /gene="fepG"
                     /locus_tag="OO230_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5692617.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14865..15680
                     /gene="fepC"
                     /locus_tag="OO230_002938"
     CDS             14865..15680
                     /gene="fepC"
                     /locus_tag="OO230_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5692618.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15677..16810)
                     /gene="fepE"
                     /locus_tag="OO230_002939"
                     /pseudo
     CDS             complement(15677..16810)
                     /gene="fepE"
                     /locus_tag="OO230_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3965 a         4841 c         4427 g         3767 t
ORIGIN      
        1 ccgaaaaaaa actgctgcaa aaatccattg aaatagatgg ttttacccaa cccattgtgg
       61 tcacacatac ggataaaaat gccatggaaa tcgtggatgg ttttcaccgc catgaaattg
      121 gtaaaggctc atcatcatta aaattacgct taaaaggtta tttgccggtg acctgtctgg
      181 aaggtacgcg taatcaacgc atcgccgcga ctattcgtca taatcgcgct cgtggacgcc
      241 atcaaatcac cgccatgtcg gaaatcgtcc gtgaacttag ccagttggga tgggatgata
      301 ataaaattgg caaagagctg ggaatggaca gcgacgaagt gctgcgactg aagcaaatta
      361 acggcctgca agagttgttt gccgaccgtc aatattcccg cgcctggacg gttaaatagc
      421 taaagctggc gcaggcgttc agctgctgcc agcaacgtcg attccttctt ggcaaaacag
      481 agacgaatca gtttatgtgg gaagggatcg gcgcaaaaca ccgacagcgg aatcgccgct
      541 acgccgtgct cctgcgtcag ccactggcaa aactcaacat catccagggt agaaaccgcg
      601 ctgtaatcca ccagcaaaaa gtatgtacct tcacacggta aaatctccag ccggctttca
      661 tttaaagcat tcaccagaat atcgcgcttc tggcgataaa agtccggtaa cgcaagataa
      721 tgctcaggtt ctgcacgtag catatcagca agcgccagct gtgccggggt attcaccgaa
      781 aaggtcagat actgatgtac cttgcgaatt tcggcgctga tgggcgctgg cgcaacacaa
      841 taacccactt tccagccggt catatgatag gtcttgccaa atgaagaaac cgccactgcc
      901 cgctcacgca gctgcggatg cgccagcaca ctggcatggc cctgttgtga aaagttgatg
      961 tgctcgtaga cttcatcgct aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa
     1021 gcggcgaaat cagcctgctg ccagacagtt gcactggggt tatgcggagt gttgaggatc
     1081 accagtctgg tgcgctcgct taataatgcg gcaaattcct gccagtcaac gcgaaaatgc
     1141 ggtggttgca gtgccatacg tttcactatt cccccagaaa gcgcgatggc gggggcgtaa
     1201 ctgtcatagc tgggatcaaa acaaatcact tcatcgccat tgcgcaccag tgcggtaatc
     1261 gccgcgtata acgcttccgt cgcccctgcc gttacggtga tatcgctatc ggcatctggt
     1321 tgatagccat acaaacgttc cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg
     1381 gtcatgggcg cgtattggtt tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa
     1441 tagcgcggac catcaaaatc aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc
     1501 gccagcgcgc tcatctgggt gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga
     1561 atcagagggt tatttgtcat ttttattgta cctgacgaag ttgcgtgttg aaggcactat
     1621 aacacgatgc cagtatttgg caatcaagac gtttagatgt ctaaataaaa caataaggac
     1681 aacacaacat gcctcacaat cctatccgcg tggtcgtcgg cccggctaac tacttttcac
     1741 atccaggaag tttcaatcac ctgcacaatt ttttcactga tgaacaactt tctcgcgcgg
     1801 tgtggatcta cggcaaacgc gccattgctg cggcgcaaac caaacttccg ccagcgtttg
     1861 gacttccagg ggcaaagcat attttgtttc gtggtcattg cagcgaaagc gatgtacagc
     1921 aactggcggc tgagtccggt gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac
     1981 tgctcgacac cgcgaaagcc ctcgcccgcc gtctcggtct gccatttgtt gccgctccga
     2041 cgatcgccgc cacctgcgca gcctggacac cgctctccgt ctggtataac gatgccggac
     2101 aggcgctgca ttatgagatt ttcgacgacg ccaattttat ggtgctggtg gaaccggaga
     2161 ttatcctcaa tgcaccgcaa caatatctgc tggcggggat cggtgacacg ctggcgaaat
     2221 ggtatgaagc ggtggtgctg gctccgcaac cagaaacgtt gccgctaacc gtgcggctgg
     2281 ggatcaataa tgcgcaggcc attcgcgacg tcttgttaaa cagtagcgaa caggcacttg
     2341 ccgatcagca aaatcagcag ttaacgcaat cattttgcga tgtggtggat gctattattg
     2401 ctggtggtgg gatggtcggt ggtctgggcg atcgttttac gcgtgtggcg gcggctcatg
     2461 ccgtgcataa cggtctgact gtcctgccgc aaaccgagaa gtttcttcac ggcaccaaag
     2521 tcgcttacgg aattctggtg caaagcgcct tgctgggtca ggatgatgtg ctggcgcaat
     2581 taaccggggc gtatcagcgt tttcatctgc cgactacgct ggcggagctg gaggtggata
     2641 tcaataatca ggcggagatc gacaaagtga ttgcccacac cttgcgcccg gtggagtcca
     2701 ttcattacct gccagtcacg ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg
     2761 aatcgtttaa agcctgacgt gcaaatttag cagcagcgcg cgccgccttt tccaccgtag
     2821 cgcgcgtcct gccgctcacg gaaaaactcc tcgtaggtca tcggcgtttg atcgggatga
     2881 ttaacccgca tatgttcgac atagttgtcg taatcaggca taccaatcat cagcttcgcc
     2941 gcctgaccta aatattttcc ggcttttgcc agtgaatcaa acatcttggc ctccgctaac
     3001 catgttgctg aaagagtgcc ggatgcggcg tgaacgcttt atccggccta caaatgctat
     3061 caacgcactc ggtcagtccc ctcgccccat cggggagagg gttagggtga gggggaaccc
     3121 tcggcactat gccaacattc gccaacatcc cccctcactc tgactttagt gcgcgccttt
     3181 cgcctgcgct acgatctcct cgacattttc cggcatcggc tcatacggcg tttctttcgc
     3241 cgttggcttc ggatctttca atgccgcaag tgccgtctta atcgagaaca gtgccaggac
     3301 caccacgacc accataaaga agatggttaa cccggcatcc agacggttgt tgaacaccag
     3361 ttgcgccagc tgtgactcag tatactgcga cggaatattg ccgctgtcga tcattgcctg
     3421 gaacttgtta gcaatgacca ggaagccgac tttcgcatcc gggctaaacg ctttcagcca
     3481 gtcagcggtc agggtacaaa tcagcagcca ggccgttggt accagcgcca cccaggcgta
     3541 acgttgacgt ttcatcttga acaacaccac ggcacagagc atcagcgcca tccctgccag
     3601 catctggttg gcaataccaa acagcggcca cagagtgtta ataccgccca gcggatcgac
     3661 cacgctctga tggaggaagt agccccacgc cagtacgcac agcgctgttg ccagcaggtt
     3721 agcaggcagt gaatcggtcc gcttcaggcc aggagacacc acgcccagca gatcctgcaa
     3781 cataaagcgc gcagcacgcg tacccgcatc taccgccgtc agaataaaca gagcttcaaa
     3841 cagaatggcg aagtgatacc agaacgccac atccatcata ccacccagcg caccgtgcag
     3901 gatgtaagcc atccccaccg ccaacgtcgg cgcaccgccc gcacgggaaa tgatcgactg
     3961 ttcacccact tcgctggcaa tctggttgag cgtatctggt gtaatgctaa agccccagct
     4021 actcaccacc tgcgcggcag aagcgactac atccgccgtc cctgccggag ccagcaccgc
     4081 cattgggctg ttcatggcaa aatacacgcc cggatcgatg atacaggcag aaactagcgc
     4141 cataatcgcc acgaaggatt ccatcaacat cccaccgtag ccgataaagc acgcctgccc
     4201 ttcgttcgcc agcatcttcg gcgtggtgcc ggaagagatc agcgcatgga agccagacac
     4261 cgcaccacag gcgatggtga taaacaggaa cgggaacagg ttaccggtcc ataccgggcc
     4321 agtgccatcg acaaatttgg tcagcgcagg catggtcagc gtcgggcgca taatcaaaat
     4381 gcctaccgcc agaccgacga tagtcccgat tttcaggaag gtcgagagat agtcacgcgg
     4441 ggccagcagc aaccacaccg gcagcaccgc cgccacaaaa ccgtagccca ccagcatcca
     4501 ggtcagctgc acgccggtaa agtcaaagta cggtgcccag gtcggacttt ctgccaccca
     4561 gccgccagag ataatggcga aaatcaggaa caccaggcca atcactgaca cttcgccaat
     4621 gcggcccgga cgcagatagc gcaggtagat tcccataaaa atcgccagcg gaatggtgaa
     4681 cgcaacggta tacgttcccc acgggctatg agtcagggct ttcaccacga tcatcgccag
     4741 tactgcaagg ataatgacca tgatcataaa gcaggctacc agcgcaatca ccccggcagt
     4801 tggccccatc tcttctttga ccagctcacc cagcgagcga ccgtcacggc gcgtagaaac
     4861 aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc accccggcga gcagccagat
     4921 catccccggc aggtagccca tttgcgctgc cagcaccggc cccaccagcg gccctgctcc
     4981 ggcaatggcc gcaaaatggt gaccgaacag cactttcttg tccgtcggca catagtccag
     5041 cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt
     5101 ggcgatatac agcccataaa aacggtaagc gatcagatag atacagaccg acgccaccac
     5161 aatccacagc gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc
     5221 tcccattaca gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat
     5281 ccgtgtgcgt gtccatagag atgttacatt ttgtttctat aacatctttg tacgatttaa
     5341 caacacggta accatgtaaa tcggagttga aaccaatatt taacttaaca atgttaactc
     5401 gatcactccg ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa
     5461 aactgccgta cccagccgac aagtgcagca acgccgcccc tgttcatcga aaacgacgat
     5521 ttcccaactc tgattttgcc gcccaagatg cagcggctgg cagacgccgc gtaccttgcc
     5581 ttcagacacc gggcgatgat gggtggcgtt aagttccgtg cccaccacac actgcccgtc
     5641 acgggtcatc ataaatccgg ccatcgatcc cagcgtttca gccagcgccg ccgacgcgcc
     5701 gccatgcagc aggccaaacg gctgatgagt acgggtatca accggcattt cggcttccag
     5761 cacatcatct cccagaaggg tatacacaat tcccagatgc gccaccattg tgttatcgct
     5821 ggtggcgttc agttcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc
     5881 gttgagccgc catcgaccac aatatcctgt agggtgatat ggctggcgag gtcagaggcg
     5941 aggaacaaaa tcgtgttggc gatctcttgt ggacgggcga ttttccccag cggaatgccg
     6001 agtttaaact gctcgccaaa gccgcgaata cgctgttctt cggcgtcatc gctcacccac
     6061 agcgtgcgtt gcatatcggt gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg
     6121 ctacccgcca gttccagccc gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg
     6181 ccataagcac tcataccaat acgcgccgtg tgtgcggcgt cggacgccac agtgacaatc
     6241 gccccgcccc gctgacggcg aaactggttc atggtttgct ggaacaggtt aaacgcaccg
     6301 ccgacgttaa ccgcaaaagt ctgctgccag tcctctttac tgagctgatc ggtcgcgccc
     6361 atgcgcaaaa ttcccgccgc attgatcagc acgtccagtc gctccgtttc agctaacagt
     6421 cgctgacata cctgcgccac ctgcccggcg tcggcaacat ccattacttc ggtcgcaaag
     6481 ggatattgct cctgagtgaa cgcttgatca aaacctgtaa ctttcgctcc cgcctcaaca
     6541 aacgtcagcg ccgtggcgta gccgatacct ttacctgcgc cggttaccca gacattttta
     6601 ccgctgaaat ccattatttc acctcgcggg agagtagctt ccaccaggcg tcgatggtcg
     6661 ggtttttcgc cagcatgaca aagtcgatat caccatgcac tttgcgccag cgcgccgcca
     6721 gcgccatcat gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt
     6781 catcggactc gtccagcaac ggcaggatca cctcacgcag cgccgctttg ctggcgggga
     6841 taggtgctgg cagtaattct tcggtcatca ccacccggcc agaacgtccg gccacatatt
     6901 tcagcgacat caaatgctcg tcacggctga aatcagccag cgcatccgcc accataaacg
     6961 gtttaatatc gcgcataaat gcgtcggttg cggtggtcat acagccaatg tgggcatata
     7021 ctcctgtaat aatcagctgg ttacgtccac tctctttgag catttgctcc agcggagaac
     7081 gatgaaacgc gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc
     7141 gatccaccac cttttgctgt tccggcgagc gggtcaggcc cggcccccac atatcattca
     7201 acagcgcccg atcttcatcg ctctgctctt tcggctgggc ggtgtaataa accgggatat
     7261 tgtgctgttt gcagtagtcg cgcagcgcag caatattcgc gatcacctgc tccatcatcg
     7321 ggcagttctc gccccagaag ctgacaaaat agtcctgcat atcatggatt aacaacgcgg
     7381 cacgttgcgg ttcaaaggcc cagtcaactt tattctgcgg aatatcgtga gactccggca
     7441 gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt
     7501 gacgccagcc actgacgtaa ttgtttttta tcgactttcc cgaccgccgt cagcggaagt
     7561 gaatccacac actccacgcg atccggtaat ttaaattcgg caataccctg ttcacgcagg
     7621 aaacgacgca cctgcaccgc gcgcagcggc tcttttacca ccagataagc acagcttttt
     7681 tcgcccatca gctcattttc catgctcacc agtgcggcat agatcactgc cggatggctc
     7741 agcagcaggt tttcgatatc ttcggcagcg atcttctcgc cgccacggtt gatctgatct
     7801 ttctcgcgcc cctgcacggt gatgtaaccc tctggatcaa tagagatcag atcgccggaa
     7861 cagtaaaaac cgttggcatc aaaggcactg gcattgtgct gcggactttt gtaatagcca
     7921 cggaaggtgt acggcccgcg cgtcatcagg cgtccaactt ccccttgcgg cagtggattt
     7981 ccttcggcat cggcaaccca cacttcatcg tccggacaca ttgggtaacc ctgggtatgg
     8041 ataattttct ccgcgctatc atcaagacgg gtgtagttca ccagcccttc cgccatgcca
     8101 aacacctgct gcaactggca gccaatctca gcgggaatac gcgctgcaag cgtggcagaa
     8161 agacgtgcgc cgccgacctg taacagtttc agcgaggcaa gctgcgcccg gctttcgcct
     8221 tcagccagcg cctgcaacca caggctgact gccggcggca ccagcgcggt aacgttaacc
     8281 tgatgttttg caatcaacgg gaagcaaagc gtggcgctgg gatcggccgc cagaacaacc
     8341 gttcctccgg caagaaagac gcccagcgat cctggcgaac tcatagtgta gttatgagcc
     8401 gccgggatcg agcacaggta gcgcgtctgt tgtgtgaact gacaaatctc ggcgctacga
     8461 cgcacgctgt agtaatagtc gttatgagtg cgcgggatca gtttcggtgt gccggtggtg
     8521 ccgccggaaa gctggaaata ggccacttca tcagcaggtg atggcgtggc agtaaaaccc
     8581 tctgccggat ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt gtgcagttgc
     8641 accacgcgaa tggaagaatg ctctgcaaca aatgtgttga ggaaatcatc cccgctaaat
     8701 agcgcatgct ggcgatcggc aatcagtaac gccggttcaa tctggctggc gtaggcgttc
     8761 agttcactac gctgatggct gaacagcgcc agcaccggcg cgacgcccag tttcagcagc
     8821 gcgaaaaagg taatgtaaag ttcagcgacg ttacccagtt gtaccagcgc ggtttcacca
     8881 ggtttaatgc cctgacggcg caaactacag gcgaggttat ccgccgcctg attcagctcc
     8941 cgataactca actgccgctc gccgtcgata accgcgatgc tgtcactcgc agcgtggcga
     9001 gtcagaatgt cggtcagtgg caaatcctgc cagtagcctt tttcccgata gcgacgggca
     9061 aactcttccg gccagcgggt gaatggaatg ctcatcctcg ctccttaatg caatccaaaa
     9121 acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc actcacccaa cggtgacgac
     9181 gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag
     9241 cggatggtca ccacccattc gccgttacct tcgctgtcac accaacccac aatgccgcca
     9301 aacagttcgc ggtcgaacgg ctccagttca gcaataacct gggtcgcggc ctgatgcggg
     9361 aaaccgctca gcgcgggggt cggatgcagc agacaggcca gagtcagtgc gttttcttgc
     9421 gaattcgctt taccttcaaa gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat
     9481 tgtggagagg agggaacgtg taactcacta ctgcgttcgc gcagtacctc tttcatcgcc
     9541 tgagtcccca gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct
     9601 tcgcgatcga gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta
     9661 aaacgctcgc cgtctttacg tagcagcagt tccgggctgg cccccagcag gacgccacca
     9721 tcagccagcg ggacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc
     9781 aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa tcaatcgtga caacaccact
     9841 ttgtcgacct gcggcgtggc ggtaagtgcg gcggcgcggg cacccatctg ttcaaacgtg
     9901 gtttgttcag gaattgccag gcgttccacc acgttcagcg actggctgcg ggtaaaacag
     9961 cgggctgagg tctgtttttc ctgacgggag aacgactgct aggattcggg aatatacagc
    10021 gacgaaggct gacgtggatc gaagggaatc gccccgacca tcaccggatt tttgatgccc
    10081 tgcgctttgg catcggcaaa cagcgcggcg agtttttgct ggaagggact gtcgggcgaa
    10141 tccccgttca cagccggttc atcgaaacgg gcgaaacatc ctgacgtcgt aaaactgcgg
    10201 tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc
    10261 tcagccagtg acgtatccat atcatcctcc acaaaatgat aaaggcttta ataatgatta
    10321 tcacttatat tttcggccgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt
    10381 aacgcaatct gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt
    10441 attgatgaat tcgcataagc gcaatgtgat gtcctgcgcc gttttgcccc ctctccccaa
    10501 agggacggat tgtgcgcttt gtcgaatttg tcattacgcc cttaacctta ttaataacag
    10561 gaagctgact tgtgagactc gccccgctct accgcaacgc ccttctatta acaggacttt
    10621 tgctttcagg aatagccgta gttcaggccg ccgactggcc gcgtcagatt actgacagcc
    10681 ggggcactca taccctggaa agccagccgc agcgtattgt ttccaccagc gtcaccctga
    10741 ccggatcact gctggcgatt gatgctccgg tgatcgccag cggcgcgacc acgccgaata
    10801 accgcgtcgc agatgaccag ggctttttac gccagtggag caaggtggcg aaagaacgca
    10861 aactgcaacg gctctatatc ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg
    10921 atctgatttt aattagcgca accggcgggg attcggcgct ggcgctgtat gatcagcttt
    10981 ccaccatcgc cccgacatta atcatcaatt acgacgacaa aagctggcag tcgctgttaa
    11041 cgcagcttgg cgaaattacc gggcatgaga aacaagcggc agagcggatt gcacagtttg
    11101 ataagcaact ggcagcggcg aaagagcaaa tcaaattacc gccgcagccg gtcactgcca
    11161 ttgtctatac cgccgctgca cacagtgcca atctctggac gccggaatca gcacaagggc
    11221 agatgctgga acaactcggc tttacgctgg cgaaattgcc cgcagggtta aacgccagcc
    11281 aaagccaggg caaacgccat gacatcattc agcttggtgg ggaaaatctg gctgcggggt
    11341 taaacggtga atcgctattc ctgttcgctg gtgatcagaa agatgccgat gcgatttatg
    11401 ccaatccact gctcgcgcac ctgcctgcgg tacaaaacaa gcaggtttat gcgctgggaa
    11461 ccgagacgtt ccgcctggat tactacagcg ccatgcaagt gctggaacgg cttaaggcgc
    11521 tgttttaagc attaactgtc ggacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc
    11581 aactccacca gcaccagcaa taacaacacg ccgataatca acaaaccaaa accgctcgcg
    11641 cttgcggaag caaccggtgt catcatcgcg cccaaaccac ccagcagcgc cgcgcctatg
    11701 gcatcgcccg tcacgttctg cgccgtccac aaaccgttaa tccgccctaa catcgcttcc
    11761 ggggtttgcg tttgcagcat tgtatattgc agcaacgaac taaccgcgct cagccagccg
    11821 aacagcgcca gacaaaccac gcctaaaatc cacatcggca tcaggccaaa cagaccaatg
    11881 gcgaggaacg atcccagcgt ggagagcagc atcaataacc ctggtcgcgc actatgtgcc
    11941 agcttcccgc tggttaacgc accaatagcc gcgccgagcg ggatcgccgc gtagagaaaa
    12001 ccaatctgtg ccgctgacat ctgccagttg tcagccagcg ccggatacag taccagcacc
    12061 gcgctcgcca tcgtcaataa accacccagc agcgggctgg caagcagaaa acgaaatcct
    12121 gccagtaatg atttcaacgg atgctcaagc ggctgcggtg gcggtggcaa ctccggaagg
    12181 cttaataacg gtaacaaggt aataaacgtg cccgccgccg ccagcccgta gttccaggct
    12241 acgccgccgg tcgccagcaa taaaccgcca atcatgggcg aaatcaccga ccccaggcgc
    12301 acggtcaaca tggtgatcgc cccggcctgc attaagtttt cacgccctac cagtgcagat
    12361 gttgccgcca gtagcgccgt aacaccaagt gatgcgaaaa aaccatccca taaaccaagt
    12421 aaatagattg ccagcaatga cggctccggc agcagtgcat taaggcacag tccaatgaag
    12481 ccaatgccac aggtgccgcg cgccagcaaa atcacttttt tgcgttcata gcgatccgcc
    12541 agcacaccgc caaccatcag gccaacaaac atcgcgccgc cggtcagcgt caccgaaagc
    12601 cccacctgcc aggtagaatg ggtcatcatc tggatctgca ccggcaccgc gacgccgagc
    12661 aaacccagag acacaattga gataaaacga gcgaggaata ctgcgcgaaa cgccgggtgc
    12721 gttttcaaca ggctgaggtt aagcagccag gattgtttat tcattacaat gccttgccat
    12781 caaatatttt aaatacctat tcttaggctg cacatgttaa catatccaaa taagatcgat
    12841 aacgataatt aatttcatta tcatggaagt tcgtatatct ggttctgttg ccgtgacacg
    12901 cgccattgcc gtgcccggat tgctgttatt actgattatc gcgacggcat taagcctgct
    12961 cattggggca aaatcactcc ccgcttccgt agtgctggag gccttctccg gcacctgcca
    13021 gagcgccgac tgcaccatcg tgctcgacgc gcggctgccg cgtacccttg ccggtttact
    13081 ggcaggcggc gcgcttggcc ttgccggggc gttaatgcaa accctcaccc gaaacccact
    13141 tgccgacccc ggcttgcttg gcgtgaacgc cggagccagc tttgccattg tgctgggtgc
    13201 ggcgctgttt ggttactcat ccgcacagga acaactggcg atggccttcg ccggggcgct
    13261 ggtggcctca ttgattgttg cctttaccgg cagtcagggt ggagggcagt taagtccggt
    13321 gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg gaaggactga ccagcggcat
    13381 cgccctgctt aatcctgacg tctacgatca actgcgtttc tggcaagccg gttcgctgga
    13441 tattcgcaat ctacatacct taaaagtggt gctgatcccg gttctgatcg ccggagcaac
    13501 tgcgctatta ctgagtcgcg cgctgaacag tttgagcctc ggcagtgaca ccgcgacggc
    13561 gctgggcagt cgcgtggcgc gcacacagtt gattggtctg ctggcgatta ccgtgctttg
    13621 tggtagtgcg acggcggtag ttggcccgat tgcctttatc ggcctgatga tgccgcacat
    13681 ggcgcgctgg ctggtaggtg ccgatcatcg ctggtcgctg cccgtcacgc tacttgctac
    13741 ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg attgtgcccg gcgaactgcg
    13801 cgtttctgtg gtcagtgcgt ttattggtgc accggtgctg atcttcctcg tgcgacgtaa
    13861 aacgcgaggt ggtgcatgat ttacgtctct cgccgattac tcatcacctg tttgctgctg
    13921 atttccgcct gtgtggttgc aggtatctgg ggattacgca gcggtgccgt cacgctggaa
    13981 atctcgcagg tattcgccgc gttgatgggc gatgcgccgc gcagtatgac gatggtggtc
    14041 accgaatggc gtttaccacg cgtgctgatg gcgctgttga ttggcgcggc gctgggcgtc
    14101 agcggcgcga tttttcagtc gctgatgcgt aacccgctcg gcagccctga cgtaatgggc
    14161 tttaacaccg gggcgtggag cggcgtgctg gtggcgatgg tgctgtttgg tcaggatctg
    14221 acggctatcg cgctggcagc aatggtgggc ggcattatca cttcgctgtt ggtctggctg
    14281 ctcgcctggc gtaacggtat cgacaccttt cggttgatta ttatcggtat cggcgttcgc
    14341 gccatgctgg tggcctttaa tacctggctg ttgctgaaag cgtctttaga aacggcgcta
    14401 acagcaggtt tgtggaatgc cggatcgctc aacggcctga cgtgggcaaa aacctcgcct
    14461 tccgcaccca tcattatatt gatgctcatt gccgccacct tgctggtgcg gcgaatgcgc
    14521 ttgctggaga tgggcgatga taccgcctgc gcgctgggcg tcagcgtcga gcgttcgcgt
    14581 ctgttaatga tgctggttgc agtggtgctt accgctgcgg caacggcgct tgccgggccg
    14641 atttccttta ttgctttagt cgcgccgcac attgcccgac gcattagcgg caccgctcgc
    14701 tgggggctga cccaggcggc gctgtgcgga gcgctgttac tgctggcggc cgttctctgc
    14761 gcccaacaac tgtttatgcc gtatcaactt ccggttggcg tcgttaccgt cagcctcggc
    14821 ggtatttacc ttatcgtctt gttaattcag gagtctcgca aaaaatgacc gaatcagtag
    14881 cccgtttgcg cggcgaacag ttaaccctgg gatatggcaa atataccgtt gcggaaaatc
    14941 tgactgtaga aattcctgat ggtcacttca cggcaattat cggaccaaat ggctgtggta
    15001 aatccacgtt actgcgtacc ttaagccgcc tgatgacgcc tgctcacggg catgtctggc
    15061 tggatggcga gcacattcaa cattacgcca gtaaagaagt tgcacgccgg attggtctgt
    15121 tggcgcaaaa tgctaccacg ccgggcgata tcaccgtgca ggagctggtg gcgcgtggac
    15181 gttatccgca tcaaccgctg tttacccgct ggcgcaaaga ggatgaagaa gcggtaacga
    15241 aagcgatgca ggccacggga ataactcatc tggcagatca aagcgtggat accctttctg
    15301 gcggacaacg ccagcgagcg tggatcgcga tggtgctggc ccaggaaacg gcaattatgc
    15361 tgctcgacga acccacgacc tggctggata tcagtcatca gattgatcta ctggaactgt
    15421 taagcgaact gaaccgcgag aaaggctata ccctggcggc ggtgctgcac gatcttaatc
    15481 aggcctgtcg ttacgccagc catttgattg cattgcggga agggaaaatt gttgctcagg
    15541 gagcgccgaa ggagattgtc actgctgaac tgattgagcg catttatggt ctgcgatgca
    15601 tgatcattga cgatccggta gccggaatgc cgcttgtggt gccgctcgga cgaacggcac
    15661 cttcaaccgc aaatagttaa actaagtggt ctgccatcat ggcatcctgt tttctggatg
    15721 ccatcgcata gcgcaataac acgctaccac aagccaccat cccgccgatc agcgcggaaa
    15781 ggatcacaat aatcgcctta cccggaccgt cttttttcac tggcaatgac gggcttaact
    15841 gatatttaaa cggcgtaaaa ttcacatcgt tgacatttgc ttttgttaat tgctcgacaa
    15901 gatactgccg attacgtaat tcaccgttca gttccgcaac gtcagtaacc gctttttcaa
    15961 tttccagttt gcgttcaata ccgtctgcgc cgagggaaat agagaaatcg gggtcatctt
    16021 taacggcctg accattactg tatacgggct ttttaattcc tgccgcgttg gcaatgtcga
    16081 gtgaataatt gaggcgctga atgtttgcat caagttgatt tttcgtttta atgcgatcct
    16141 gagccagttt ttctttttca aactgggttt tgatctccag tttattacgg acgttttcta
    16201 tcgactcttt caccaccaac gcagagatat aatcgatata cccgctcaaa acggtctgcg
    16261 cctctttact ggttggcgcg gtaaaactta gcgtccagga tgtatacagt gacggttcat
    16321 cttttttctt actagcattg tcatcaaccg ctttcatttt ttcgctcaat gcaacaatag
    16381 cgcgatgcaa atccagttca tcgattttcg cctcttttaa ttagtccatc acataaggtg
    16441 atgaacgcag gtactcttcc agcaagctaa cagactgaaa cttcttgata aacaggttaa
    16501 atgcaccgcc acggtcgata ttaacatcga gatcaagaac gcggagtttg gtaaaggttt
    16561 tctccaggtc ctgccactga atggcttcgg cgggagtaat aacggctgag ctggtccatt
    16621 tttgcggcag gatgaaagag atcagtaaac ctgcgcaggc aaatgcaaaa acgaccgcca
    16681 tgaccgtttt tttggcccgc cataaaactt cgattagatt aagtaaatca atttcattat
    16741 tactgggcga cgccagagga tactcagaaa aatgagcgtc acttccctgt ttaatattca
    16801 atgatgacat ggggtataac ctgaaaatga gtccaatagg ggaaaatctc cacggcgctc
    16861 actttaccag ttggtgagaa ttatccgaag ctgaagtttg taaattcctt ccactgaatg
    16921 caacattcac tctatttaag agaaatattc atcaaaccta taaatcaaac aaataaaaaa
    16981 cccataatta ttttctgtaa
//
LOCUS       DAJTPG010000086        16996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTPG010000086 DAJTPG010000000
VERSION     DAJTPG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(30..221)
                     /locus_tag="OO230_002940"
     CDS             complement(30..221)
                     /locus_tag="OO230_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5692619.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(326..562)
                     /locus_tag="OO230_002941"
     CDS             complement(326..562)
                     /locus_tag="OO230_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5692620.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(680..2119)
                     /gene="rsmF"
                     /locus_tag="OO230_002942"
                     /pseudo
     CDS             complement(680..2119)
                     /gene="rsmF"
                     /locus_tag="OO230_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2199..4819)
                     /gene="yebT"
                     /locus_tag="OO230_002943"
                     /pseudo
     CDS             complement(2199..4819)
                     /gene="yebT"
                     /locus_tag="OO230_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4788..6071)
                     /gene="yebS"
                     /locus_tag="OO230_002944"
     CDS             complement(4788..6071)
                     /gene="yebS"
                     /locus_tag="OO230_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5692621.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6201..6698
                     /gene="msrC"
                     /locus_tag="OO230_002945"
     CDS             6201..6698
                     /gene="msrC"
                     /locus_tag="OO230_002945"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5692622.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6795..7493
                     /gene="proQ"
                     /locus_tag="OO230_002946"
     CDS             6795..7493
                     /gene="proQ"
                     /locus_tag="OO230_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5692623.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7513..9561
                     /gene="prc"
                     /locus_tag="OO230_002947"
     CDS             7513..9561
                     /gene="prc"
                     /locus_tag="OO230_002947"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5692624.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9753..10634
                     /gene="htpX"
                     /locus_tag="OO230_002948"
     CDS             9753..10634
                     /gene="htpX"
                     /locus_tag="OO230_002948"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5692625.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10680..12053)
                     /locus_tag="OO230_002949"
     CDS             complement(10680..12053)
                     /locus_tag="OO230_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692626.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12230..13021
                     /gene="kdgR"
                     /locus_tag="OO230_002950"
     CDS             12230..13021
                     /gene="kdgR"
                     /locus_tag="OO230_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5692627.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13164..13403)
                     /locus_tag="OO230_002951"
     CDS             complement(13164..13403)
                     /locus_tag="OO230_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692628.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13562..13705
                     /gene="mgrB"
                     /locus_tag="OO230_002952"
     CDS             13562..13705
                     /gene="mgrB"
                     /locus_tag="OO230_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5692629.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13780..14067
                     /locus_tag="OO230_002953"
     CDS             13780..14067
                     /locus_tag="OO230_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5692630.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14737..14880
                     /locus_tag="OO230_002954"
     CDS             14737..14880
                     /locus_tag="OO230_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5692631.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14893..15102
                     /gene="cspE"
                     /locus_tag="OO230_002955"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14893..15102
                     /gene="cspE"
                     /locus_tag="OO230_002955"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5692632.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15268..16077
                     /gene="rlmA"
                     /locus_tag="OO230_002956"
     CDS             15268..16077
                     /gene="rlmA"
                     /locus_tag="OO230_002956"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5692633.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16074..16640)
                     /gene="mntP"
                     /locus_tag="OO230_002957"
     CDS             complement(16074..16640)
                     /gene="mntP"
                     /locus_tag="OO230_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5692634.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4487 a         4115 c         4287 g         4107 t
ORIGIN      
        1 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
       61 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      121 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      181 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      241 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      301 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      361 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      421 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      481 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      541 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      601 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      661 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      721 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      781 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
      841 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
      901 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
      961 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1021 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1081 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1141 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1201 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1261 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1321 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1381 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1441 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1501 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1561 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1621 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1681 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1741 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1801 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     1861 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     1921 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     1981 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2041 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2101 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2161 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2221 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2281 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2341 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2401 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2461 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2521 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2581 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2641 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2701 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2761 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     2821 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2881 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     2941 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3001 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3061 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3121 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3181 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3241 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3301 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3361 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3421 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3481 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3541 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3601 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3661 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3721 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3781 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     3841 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     3901 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     3961 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4021 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4081 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4141 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4201 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4261 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4321 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4381 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4441 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4501 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4561 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4621 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4681 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4741 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4801 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     4861 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     4921 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     4981 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5041 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5101 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5161 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5221 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5281 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5341 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5401 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5461 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5521 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5581 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5641 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5701 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5761 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     5821 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     5881 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     5941 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6001 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6061 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6121 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6181 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6241 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6301 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6361 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6421 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6481 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6541 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6601 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6661 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6721 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6781 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     6841 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     6901 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     6961 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7021 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7081 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7141 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7201 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7261 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7321 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7381 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7441 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7501 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7561 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7621 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7681 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7741 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7801 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     7861 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     7921 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     7981 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8041 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8101 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8161 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8221 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8281 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8341 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8401 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8461 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8521 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8581 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8641 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8701 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8761 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     8821 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     8881 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     8941 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9001 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9061 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9121 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9181 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9241 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9301 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9361 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9421 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9481 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9541 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9601 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9661 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9721 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9781 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
     9841 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
     9901 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
     9961 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10021 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10081 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10141 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10201 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10261 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10321 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10381 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10441 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10501 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10561 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10621 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10681 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10741 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10801 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    10861 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    10921 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    10981 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11041 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11101 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11161 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11221 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11281 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11341 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11401 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11461 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11521 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11581 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11641 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11701 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11761 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    11821 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    11881 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    11941 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12001 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12061 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12121 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12181 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12241 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12301 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12361 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12421 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12481 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12541 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12601 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12661 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12721 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12781 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    12841 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    12901 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    12961 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13021 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13081 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13141 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13201 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13261 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13321 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13381 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13441 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13501 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13561 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13621 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13681 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13741 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13801 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    13861 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    13921 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    13981 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14041 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14101 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14161 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14221 aagatatttt cctcattaat ttattaggat gtttacatca gatttgtgat taagcgtggt
    14281 attatttatt agcgaaacgt ttctctcttg atttttttgc tcactcatca atttttctta
    14341 ttttaaattt acaatccctt tggggattga cttctcttta gggtaattga tagccgttaa
    14401 ctgactgttt tatgagaaaa agtgatataa ctttttattc attgcatagc agaaaatgtg
    14461 atattgcacg cactatgtaa taacttctcc ctctggcctg gaacaactga acttattgaa
    14521 ctatgttaga aactacgcca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg
    14581 atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat
    14641 ttgtgcgtct gatcgagata tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat
    14701 aaaagatttc gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa
    14761 gtcctgagta aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc
    14821 tcatgcagta atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa
    14881 ggaatttttc aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt
    14941 tttggcttca ttactccggc tgatggcagc aaagatgtgt tcgtacactt ctccgctatc
    15001 cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac
    15061 ggccagaaag gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga
    15121 agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactatg
    15181 ctttatcttc gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc
    15241 ggcctttagt atcctgccgg agttgtcatg tcgttttcct gtccactttg ccatcagcct
    15301 ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac atcagtttga tatggcgaaa
    15361 gaagggtatg tcaatctgct gcccgttcag cataaacggt ctcgtgatcc gggcgacagc
    15421 gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt
    15481 gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa aggccacggc ggtgctggat
    15541 attggctgtg gtgaagggta ttacacacac gcatttgccg atgcgttgcc cgaaatcacc
    15601 acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag
    15661 gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc
    15721 ataatacgta tttacgcgcc gtgtaaagca gaagaattag cacgagtagt gaagcccggc
    15781 ggctgggtca ttactgccac gccgggaccg cgacatttga tggagctgaa ggggctgatt
    15841 tacaatgaag tacatcttca tgcacctcat gcagaacaac tggaaggttt tacattacag
    15901 cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt cgcattattg
    15961 cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa
    16021 gaagtgttcg actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg
    16081 tggaagtgcg tccagaggat ctggacgccg atgccgatca gcaccagccc gccgagaatt
    16141 tccgcttttt tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgtcgac
    16201 ataatcaagg ttgcacaacc aatggccaat gcggtcgcga taatgttgac ctgaaggaaa
    16261 gcaagaccaa cacccacagc catggcatcc aggctggtgg caatcgcggt ggttaccagt
    16321 agccagaaac cgtgccgacg gcgcggctct tcatcttcat catctgcgcc acgaaaaccc
    16381 tcaataatca ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca gtgattccat
    16441 tcaaggacaa accggctggc taacatgccc attccccagc cgatcagcgg cgtcagggtt
    16501 tcgacggcac caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg
    16561 ggggcacctt taccgattga tgcagcaaat gcatccatcg acataccaaa cgcaagaaga
    16621 acagtagcag tgatattcat gacaacgtcc tgaccggggt atccataaaa cacatcatcg
    16681 cccccagcaa acatgcatac atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg
    16741 catagcgatt gatgtgcata tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca
    16801 cgttttgcaa cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc
    16861 aaatgagggc gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat
    16921 ttcattgctt tattgataac gattttcatt tatgtttata tatcaatgaa acgttaaaat
    16981 aaataacaaa taaggg
//
LOCUS       DAJTPG010000087        16391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTPG010000087 DAJTPG010000000
VERSION     DAJTPG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO230_002958"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO230_002958"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5692635.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO230_002959"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO230_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5692636.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO230_002960"
     CDS             2339..2554
                     /locus_tag="OO230_002960"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692637.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO230_002961"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO230_002961"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5692638.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO230_002962"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO230_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5692639.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO230_002963"
     CDS             5920..6417
                     /locus_tag="OO230_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5692640.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO230_002964"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO230_002964"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5692641.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO230_002965"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO230_002965"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5692642.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO230_002966"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO230_002966"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5692643.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO230_002967"
     CDS             9102..9665
                     /locus_tag="OO230_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5692644.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO230_002968"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO230_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5692645.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO230_002969"
     CDS             complement(10257..11237)
                     /locus_tag="OO230_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5692646.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO230_002970"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO230_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5692647.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO230_002971"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO230_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5692648.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO230_002972"
     CDS             12540..12830
                     /locus_tag="OO230_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5692649.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO230_002973"
     CDS             12838..13431
                     /locus_tag="OO230_002973"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5692650.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO230_002974"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO230_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5692651.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14875..15838
                     /locus_tag="OO230_002975"
                     /pseudo
     CDS             14875..15838
                     /locus_tag="OO230_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15883..16386
                     /locus_tag="OO230_002976"
     CDS             15883..16386
                     /locus_tag="OO230_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5692652.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4220 c         4124 g         3988 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactat atcattcatt
    14701 gattataaat atgtacaacc tggcgcacaa atttaatatt taatttctga ctttatttca
    14761 agttgtgata aacatagaca agttgtcaga caggttaacc aatttgtcgc acaaatcact
    14821 gataagcaca attagatatc aaccaatttc catccttcaa cagggagttt ttttatgaaa
    14881 atgaggtcat ttacccgctc attagtctgc gcatccttgc tggctctggt tagcactggc
    14941 gtaaacgccg cagaaaaagt ggcgttaaag ttagcacaca acctggaaag aagtcatgtt
    15001 gtacatcaga gttttgaaga gctggcgaaa gaggtaaaac agctgtcaaa aggtaatatg
    15061 gtgatccgta tttatcccag cagccagatg ggtaatgcgc gagaaactat ggaattgttg
    15121 caaaatggcg cactggatat gaccaaaggt tccgccagtg atctggagtc ttttgataat
    15181 atttatgcta tctataattt gccgttctta ttaagttgtt tttggtgaag ttggtaaaga
    15241 aattatggac tcaacaaaag acaaaggctt ttttgcttta tcagcttatg tagcgggtac
    15301 tcgcagtttc tatgcgaaaa aaccgattac aaagccagaa gatcttaaag gactgaaaat
    15361 tcgtgttcag ccaagtccga ccaccatcaa aatgattgaa ctgatgggtg gttcacccac
    15421 tccaatttca tttggcgaag tttatactgc gatgcagcaa ggtgtagtag atggcgcaga
    15481 aaataacgtg ccttcctgga tgcagactcg acatattgaa attgccaaag ttttttctga
    15541 agatgaacac gcctcaattc ctgacttttt ggttatctcc accaaaacat ggaataaatt
    15601 aaccccagaa cagcaacaaa tcctcgaaac agcggcgaag aaatccgaag cttatcaaca
    15661 aaaattgtgg gaaaaaattg acgctgacac acgtgctcag gcgaaagcta tggggggtga
    15721 aattgtaaaa gtggataaag cgccattccg taaagccgtg caacctctgt ttgacgactt
    15781 caaaaaagat ccaaaacaag ctgctttgtt agagaaattc gacaacgctg ctcaataata
    15841 ttccgggcca gcctctggcc ctgattttcc aggatgctta caatgatatt taaccgcctg
    15901 aaacttgcgg tagaccgcgt cattgccacg ttttctgtcg ccgtaatgct tgctctggtt
    15961 gtatgcgtag tctggcaagt atttagccgc tacgtactta accagccaag tacattaacc
    16021 gatgagctgg cacgcttttt gatgatctgg gtaggattat taggtgctgc atataccgtc
    16081 ggcgcgcagc gacatctctc tattgattta tttgctctcg ccctgaataa gcgcaaacaa
    16141 ttacttctta gtatagtcat taacgttctc attttaggtt ttgccggttc ggtcattgtg
    16201 actggtggcc tgaaattaat tgataaaaca ctggcaacat cacaggtatc tgccgctatg
    16261 caaataccga tgggatacgt atatatcatt ctgccattaa gtggagtggt aatgatgttt
    16321 tatgccttgt gttttattaa tcaaagcatt caacaactga aacaacctgc gcaggaggcc
    16381 agttaatgga t
//
LOCUS       DAJTPG010000088        15930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTPG010000088 DAJTPG010000000
VERSION     DAJTPG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO230_002977"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO230_002977"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5692653.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO230_002978"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO230_002978"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5692654.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO230_002979"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO230_002979"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5692655.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO230_002980"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO230_002980"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5692656.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="OO230_002981"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="OO230_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="OO230_002982"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="OO230_002982"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5692657.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="OO230_002983"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="OO230_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="OO230_002984"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="OO230_002984"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5692658.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="OO230_002985"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="OO230_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5692659.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10211)
                     /locus_tag="OO230_002986"
     CDS             complement(8973..10211)
                     /locus_tag="OO230_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5692660.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="OO230_002987"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="OO230_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5692661.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO230_002988"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO230_002988"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5692662.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO230_002989"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO230_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5692663.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="OO230_002990"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="OO230_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5692664.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14682..15584
                     /locus_tag="OO230_002991"
     CDS             14682..15584
                     /locus_tag="OO230_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5692665.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15731..15930
                     /locus_tag="OO230_002992"
                     /pseudo
     CDS             15731..15930
                     /locus_tag="OO230_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3953 a         4119 c         3855 g         4003 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa
    14641 gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact
    14701 tgaaaaagct cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
    14761 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
    14821 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
    14881 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
    14941 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
    15001 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
    15061 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
    15121 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
    15181 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
    15241 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
    15301 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
    15361 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
    15421 tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag
    15481 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt
    15541 tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca
    15601 gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt
    15661 acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga
    15721 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
    15781 agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
    15841 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
    15901 aatctgcgtt ttgtttatca actgggaggc
//
LOCUS       DAJTPG010000089        15517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTPG010000089 DAJTPG010000000
VERSION     DAJTPG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO230_002993"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO230_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5692666.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO230_002994"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO230_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5692667.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO230_002995"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO230_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO230_002996"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO230_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5692668.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO230_002997"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO230_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5692669.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO230_002998"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO230_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5692670.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO230_002999"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO230_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5692671.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO230_003000"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO230_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5692672.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO230_003001"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO230_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5692673.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO230_003002"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO230_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5692674.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO230_003003"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO230_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5692675.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO230_003004"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO230_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5692676.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO230_003005"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO230_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5692677.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO230_003006"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO230_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5692678.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO230_003007"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO230_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5692679.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO230_003008"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO230_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5692680.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO230_003009"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO230_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5692681.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO230_003010"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO230_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5692682.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO230_003011"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO230_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5692683.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO230_003012"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO230_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5692684.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO230_003013"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO230_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5692685.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO230_003014"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO230_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5692686.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO230_003015"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO230_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5692687.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO230_003016"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO230_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5692688.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO230_003017"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO230_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5692689.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO230_003018"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO230_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5692690.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO230_003019"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO230_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5692691.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO230_003020"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO230_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5692692.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO230_003021"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO230_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5692693.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO230_003022"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO230_003022"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5692694.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO230_003023"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO230_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5692695.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4006 a         3641 c         4130 g         3740 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctg
//
LOCUS       DAJTPG010000090        15497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTPG010000090 DAJTPG010000000
VERSION     DAJTPG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..73)
                     /locus_tag="OO230_003024"
     CDS             complement(<1..73)
                     /locus_tag="OO230_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5692696.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO230_003025"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO230_003025"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5692697.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO230_003026"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO230_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5692698.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO230_003027"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO230_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5692699.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO230_003028"
     CDS             2703..2939
                     /locus_tag="OO230_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5692700.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO230_003029"
     CDS             2942..3799
                     /locus_tag="OO230_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5692701.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OO230_003030"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OO230_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO230_003031"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO230_003031"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5692702.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OO230_003032"
     CDS             complement(6414..7310)
                     /locus_tag="OO230_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5692703.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OO230_003033"
     CDS             complement(7359..7598)
                     /locus_tag="OO230_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5692704.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OO230_003034"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OO230_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5692705.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="OO230_003035"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="OO230_003035"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5692706.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="OO230_003036"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="OO230_003036"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5692707.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="OO230_003037"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="OO230_003037"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5692708.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..12879
                     /locus_tag="OO230_003038"
     CDS             10765..12879
                     /locus_tag="OO230_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5692709.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12889..14181
                     /locus_tag="OO230_003039"
     CDS             12889..14181
                     /locus_tag="OO230_003039"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692710.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15131..15223
                     /locus_tag="OO230_003040"
     CDS             15131..15223
                     /locus_tag="OO230_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5692711.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15267..>15497)
                     /locus_tag="OO230_003041"
     CDS             complement(15267..>15497)
                     /locus_tag="OO230_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5692712.1"
                     /translation="PAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNI
                     LAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3551 a         4098 c         4073 g         3775 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctgg
//
LOCUS       DAJTPG010000091        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTPG010000091 DAJTPG010000000
VERSION     DAJTPG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO230_003042"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO230_003042"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5692713.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO230_003043"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO230_003043"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5692714.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO230_003044"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO230_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5692715.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO230_003045"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO230_003045"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5692716.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO230_003046"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO230_003046"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5692717.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO230_003047"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO230_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5692718.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO230_003048"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO230_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5692719.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO230_003049"
     CDS             complement(8064..9206)
                     /locus_tag="OO230_003049"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5692720.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO230_003050"
     CDS             complement(9215..10228)
                     /locus_tag="OO230_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5692721.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO230_003051"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO230_003051"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5692722.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO230_003052"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO230_003052"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5692723.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO230_003053"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO230_003053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5692724.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO230_003054"
     CDS             complement(12826..13950)
                     /locus_tag="OO230_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5692725.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO230_003055"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO230_003055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5692726.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4382 a         3445 c         3144 g         4373 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccgatac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaaa
//
LOCUS       DAJTPG010000092        15078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTPG010000092 DAJTPG010000000
VERSION     DAJTPG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO230_003056"
                     /gene_synonym="yiiD"
     CDS             complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO230_003056"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5692727.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO230_003057"
     CDS             complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO230_003057"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5692728.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1939..2811)
                     /locus_tag="OO230_003058"
     CDS             complement(1939..2811)
                     /locus_tag="OO230_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5692729.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO230_003059"
     CDS             complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO230_003059"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5692730.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3503..4288)
                     /locus_tag="OO230_003060"
     CDS             complement(3503..4288)
                     /locus_tag="OO230_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5692731.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO230_003061"
     CDS             complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO230_003061"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5692732.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5386..6282
                     /gene="yihU"
                     /locus_tag="OO230_003062"
     CDS             5386..6282
                     /gene="yihU"
                     /locus_tag="OO230_003062"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5692733.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6306..7184
                     /gene="yihT"
                     /locus_tag="OO230_003063"
     CDS             6306..7184
                     /gene="yihT"
                     /locus_tag="OO230_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5692734.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7201..8442
                     /gene="yihS"
                     /locus_tag="OO230_003064"
     CDS             7201..8442
                     /gene="yihS"
                     /locus_tag="OO230_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5692735.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8580..9482
                     /locus_tag="OO230_003065"
     CDS             8580..9482
                     /locus_tag="OO230_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5692736.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9681..11717
                     /gene="yihQ"
                     /locus_tag="OO230_003066"
     CDS             9681..11717
                     /gene="yihQ"
                     /locus_tag="OO230_003066"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5692737.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11763..13137
                     /locus_tag="OO230_003067"
                     /pseudo
     CDS             11763..13137
                     /locus_tag="OO230_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13180..14583
                     /locus_tag="OO230_003068"
     CDS             13180..14583
                     /locus_tag="OO230_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5692738.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14651..14980
                     /gene="ompL"
                     /locus_tag="OO230_003069"
                     /pseudo
     CDS             14651..14980
                     /gene="ompL"
                     /locus_tag="OO230_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
     gene            complement(14996..>15078)
                     /locus_tag="OO230_003070"
     CDS             complement(14996..>15078)
                     /locus_tag="OO230_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5692739.1"
                     /translation="KSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         3699 a         3880 c         3756 g         3743 t
ORIGIN      
        1 gatttaatag cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa
       61 cagaataact ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac
      121 ggtttaagag caaacataca acataatgat tcataacata cagagcagta aatgaatgat
      181 actgacaatg tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa
      241 aatatttatg aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      301 ctgaactaat atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata
      361 atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa
      421 aaatttataa agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg
      481 gaatgaatga gagaggcagc accgcccctc tccataagct actcttcttc gttcccgccc
      541 tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca
      601 cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc
      661 gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg
      721 ctaatcggtt tgctgtagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg
      781 cgttcacgca gcatcagcca gataagcccc caaccggtga gcgtcgccag cgagaataaa
      841 ctcccggcaa acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata
      901 aatttttgcc cggtatattg ttgaatacgc acgcccattt tttcactaag cgggatatgt
      961 tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga
     1021 gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt
     1081 tctccctgat taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta
     1141 caggtcacgc gcttaacgcc ttcctgacgc gccaccgact ccagggtcat cgccatcagc
     1201 gttcctaacc ctttgtcctg cacgtccggg tgaacgacca taaagcgaat ggacgcttca
     1261 ttgtcggcat taatatacaa tcggcctacc gccaccagat taccctgctc gtcgacgacc
     1321 atctggtgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg
     1381 ggcttacgca acatttccca gcgaaagtga tagtaacgct ctaactcttc ttctgtttgt
     1441 ggaacctgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc
     1501 tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat
     1561 cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat
     1621 agtcatataa cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc
     1681 cccgttcggt atctgcggcg agggtaaact gggaaaccac cagtacactg ccgcccgcct
     1741 gttgcacgtt gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagcacac
     1801 gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca
     1861 aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta
     1921 cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag
     1981 tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt
     2041 ccagtagacc caaacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata
     2101 tgacgggaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc
     2161 ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct
     2221 gtacagcaac cagaaggaga tccacgacaa cagcaacgga aaaatacgca gcacgttgtc
     2281 gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag
     2341 actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg cgaacgagta
     2401 aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta
     2461 catcaacaat aacgcggtga cgatcagccc gcacgcccca acggcggtca tcttgttgga
     2521 attagcaaca aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt
     2581 ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa aagcggcgaa
     2641 aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt
     2701 acctgccagg gttgtcatgt tgtcctcatc aatacgttgc cagagtagtt ttagccaggc
     2761 ccatagtgga cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc
     2821 gcgaaatagt ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga
     2881 ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa
     2941 ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct
     3001 tgcgacagat agatatggtc agcagcatca cgaatttccg ggtattcttc cggccagaag
     3061 gtggtatgca ggcggttggt attggaaagc accaccacgc gatgcccctg ctcacgcagt
     3121 ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga
     3181 gagaactgct cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct
     3241 tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct cccccatatg aaaactcttc
     3301 ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg ctcccagcac acggttaaag
     3361 tcgatatcga caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc
     3421 gccacaagaa attaactgta gcgggaaagg atgggtttgg ctatgtggca aggtatgaag
     3481 ccagattaac ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt tcaatttggc
     3541 tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga
     3601 cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga
     3661 ccagcagaat atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgtttacat
     3721 caaactccat caacgcgcca tcgctctcaa tcgccccaac gcttgttacc agataatcag
     3781 cacgaaaatc agccacaaag gacgccgctg aagggccaat gatcccgcta ttatgagagc
     3841 gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca
     3901 cacgcaggct gttggtgatt atccgcaaat gattatggtt aagtaacgcc cgggcaacat
     3961 gctcaacagt cgtaccaatg gtgataaata ttgttgaacc atcaggaata tagtctgcca
     4021 ccgcttcggc aatcgctttt tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg
     4081 tattaacgac gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct
     4141 cgcttaattt acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca
     4201 gctcatcaat attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg
     4261 ggttaccggt tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat
     4321 tttatacaaa aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc
     4381 caccgggacg tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta
     4441 aatccccact tgttgccagc gccaccgcca aagcgccgtg aaaaacatct cccgcacctg
     4501 tggtatctac cacatcaact ttgaaggccg gttgatgctg acgcccacca ttttccagcc
     4561 agtcgcagcc tgcgctaccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct
     4621 gttttagcgc actggccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa
     4681 aggccgcgtg atcgcttaat gccaccagct cactgatatc ctgcggcgta atgtccccgt
     4741 ccagaacggt catcacaccc gcctgacggg ccagggtgaa ggctttttta gcgccgtcgt
     4801 gccagcgtac atctgccagc acaacatccc actgagagaa atcaatttcc tccaaccact
     4861 ctgcgtcagg cagcagatcc gggctggggt agttaatgat tatccgctcg cctttggtat
     4921 ccaccatgat ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt
     4981 taacgcccca ggattccagc tctgccagca ggctgttgcc cgcgtcgtca tcacctacgc
     5041 gaccaataaa atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg
     5101 gcccgccacc aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaacc
     5161 cttccacgta atagatgcga tccatcacgg ttatacctac acaagcaaca cgaatcatgg
     5221 tcattcctta aacattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga
     5281 cggctgtcaa ttttttgacg ataaattaca aattcgatca aaaacagaca agcaaagcat
     5341 gtcataaatg acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg
     5401 tttatcggtt taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat
     5461 caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg
     5521 actcccgccg ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg
     5581 ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta
     5641 tctaccgatg cgctggtcat tgatatgtcc accatccatc cgctgcaaac cgataaattg
     5701 attgccgata tgcaagccaa aggcttcagc atgatggatg ttccggtagg ccgtacttct
     5761 gcaaatgcca ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa
     5821 cgtgccacac cgatcctgat ggcgatgggc agtgagttga tcaacgctgg cggtccgggc
     5881 atggggatcc gcgttaagct catcaacaac tacatgagca tcgcgctcaa tgcgctttcg
     5941 gcagaagccg ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg
     6001 atgagcggta ccgccgccgg taaaggtcac ttcaccactt cctggccaaa caaagtcctc
     6061 agcggagatc tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc
     6121 cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg gggccgcctc acgggaggtt
     6181 tatagccagg cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggaacag
     6241 gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata
     6301 aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc atcgggcggt tttgccatgc
     6361 tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg gcacccgctc
     6421 cggtagccga tagcgtttta actgatttca aagttaacgc tgcaaaggcc ctctcgcctt
     6481 atgcctcggc gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg
     6541 cgattgccaa aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta
     6601 ttccggtcga tagcgtggtt attgaccgca aaatcaatcc gctacagatc aaacaagacg
     6661 gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgtc
     6721 tggatatggt gaaagagttc aacgaactgt gccactcaca cggtctggta agcatcattg
     6781 agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcgatcatcg
     6841 atgccgccaa agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt
     6901 atggcaaagg tccgcaacaa gagcttctct gtgcttcaca acgtctgaat gaccatatca
     6961 atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa actgttcccg cgtgccgtac
     7021 gcgtggcaat gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc tgggcatcgg
     7081 tcgtcggttt accagacaac gagctgatgc tccgtgacgt ttgcgcaccg aaattacaac
     7141 aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag gataaaagga
     7201 atgaaatggt ttaacaccct aagccacaac cgttggctgg aacaggaaac cgaccgcatc
     7261 tttgattttg gtaaaaattc cgtagtgccg actggttttg gctggttagg caataaaggg
     7321 caaatcaaag aagagatggg cacccatctg tggatcacag ctcgtatgtt gcacgtttat
     7381 tccgttgctg cggcgatggg tcgacctggc gcttacgcgt tggttgatca cggtattaaa
     7441 gccatgaacg gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg cgtgaatgac
     7501 gagggcgtgg tggatgcctc caaacagggc tatcaacact tctttgctct gctgggtgcc
     7561 gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta caccattgag
     7621 attatcgaga aatatttctg gagcgaagaa gagcagatgt gcctggagtc ctgggacgaa
     7681 gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc ggtggaagct
     7741 ttcttgattg tttatgacgt cactcacgac aaaaaatggc tggatcgcgc gattcgcgtg
     7801 gcttccgtga ttatccacga cgtcgccaga aataatcatt atcgcgttaa cgaacacttc
     7861 gatacccagt ggaatccgct gccggattac aacaaagata acccggcgca tcgcttccgc
     7921 gcgttcggag gtacaccagg ccactggatc gaatggggcc gtttaatgct gcacatccat
     7981 gctgccctgg aagcccgttg cgaacaacca ccagcatggc tgctagaaga tgccaaaggt
     8041 ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg gagcggacgg gattgtttat
     8101 accgttgact gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc tatcgtcgaa
     8161 gcaatgggta cggcctacgc gctctacacc gtcactggcg atcgccagta tgaaacctgg
     8221 tatcaaacat ggtgggagta ctgcattaag tacctgatgg actatgaaaa tggttcctgg
     8281 tggcaggagc tggatgcgga caataaggtc atcaccaaag tctgggacgg caaacaggat
     8341 atttatcacc tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc cggcatggct
     8401 ccagcggttg cagcgggtct gctggatatt aatgcgaaat aactgatctg aaaatagggt
     8461 gcgggttttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccggtgcag
     8521 tttttgggtt tgacatcagc ctcacgggag gctgtatgtc gttaattaag gtgccttgta
     8581 tgcaaatcac caatatgcat tgcagtggac agaccgtttc acttgccgcg ggggattatc
     8641 acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag ggttgtcatt
     8701 tagtcatccc gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa gtgcttattc
     8761 cgtggccgaa ccggatcgcc aacggctgtt accgttatca ggggcaggaa tatcagttac
     8821 ccattaatga acatagttca aaagccgcta ttcacgggct gcttgcctgg cgagactggc
     8881 aaattagtga attaaccacc accagcgtta ccctgacagc ttttctgccg cccagttacg
     8941 gctatccgtt tatgctggct tcacaagttg tttattcgct caacgcacac acgggtcttt
     9001 ccgttgagat cgcaagccag aatatcggca ctgtcgcagc gccttatggc gtgggtattc
     9061 atccttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag cttccggcaa
     9121 accaggttta tgccgtcgat gaacacgtaa atccgacaac gcttcaccac gttgatgagc
     9181 tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac cacaccttta
     9241 aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc acagcaggcg ctttcggtaa
     9301 gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg tgaaaaatta cagcgtcagg
     9361 gactggcagt tgaacctatg agttgcccgc ctaacgcctt taattcaggt atcgacttat
     9421 tattactgga accaggaaaa ccgcaccgcc tgttttttaa tatttacgga cagagaaaat
     9481 aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta aaggcaacat
     9541 tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt tttatatatc
     9601 agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca atctcgttct
     9661 caactctatg gagattagtt atggatacgc cacgtccaca gttattagat tttcaatttc
     9721 atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta acccataaca
     9781 aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg ttccgcggta
     9841 atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc atcgtcagcc
     9901 agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc gccacgctga
     9961 atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac aaccttaacc
    10021 acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac ggctgcggcg
    10081 aacagttttc ctacttcgat ctgcgcggca aaccgttccc gctatggacc agtgaacaag
    10141 gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg
    10201 gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg cagaagtatt
    10261 actgccatgt tgataacagt tgctacatga acttcgactt tagcgccccg gaataccatg
    10321 aactggcgct gtgggaagac aaagctacgc tacgttttga atgtgctgac acatacatct
    10381 ccctgctgga aaaattaact gccctgctgg gacgccagcc agaactgccc gactggattt
    10441 atgacggggt aacgctcggc attcagggcg ggacggaagt gtgccagaag aaactggaca
    10501 ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg tctgggatcc
    10561 gtataacctc ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc
    10621 cgcaactgga ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc ctggcctata
    10681 tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa cacggctatc
    10741 tagcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt tacggcggcg
    10801 ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc aaaaagaaca
    10861 tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg cccaccgaca
    10921 cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct gcgctgtggg
    10981 cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc ctcttcttta
    11041 tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg ggcgaccaga
    11101 acgtggactg gagtctcgac gatggcctgg cgtccgtagt cccggcggcg ctgtcgctgg
    11161 caatgaccgg acatggtctg catcacagcg acattggcgg ttacaccacc ctgtttgaga
    11221 tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc acgccgatga
    11281 tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc gatgcggaaa
    11341 ctatcgccca ttttgcccgt atgactaccg ttttcaccac cctgaaacct tatctgaaag
    11401 aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg ttcctgcatt
    11461 acgaagacga tgcacatacc tattccctga aatatcagta cctgttaggc cgcgacattc
    11521 tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac gctctatctg ccggaggata
    11581 actgggtaca tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc gttaatgcgc
    11641 ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca ctgttcgcgt
    11701 cgttaaacag catctaatca catccgcccg tggggaaact catgggcaaa gggagaacat
    11761 cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt aaagagaaac
    11821 tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt acgctttatc
    11881 ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc attatctttt
    11941 tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg ttggattccc
    12001 gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg tcattcccgg
    12061 tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc ggtaaaacgg
    12121 tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg aactgctcct
    12181 acggcgcaat ggtcccggca atcaccaaaa accccaacga acgcgcctca ctggcggcat
    12241 ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc gtgccagtta
    12301 tgaatcttat cgaaggtaat cagaaacttg gctatatctt cgccgccacg ctgttttcac
    12361 tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa
    12421 cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc gcaattgccg
    12481 gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg gcgtttaacg
    12541 tcaagctcgc cattcaggtc tattacaccc agtacgtact taacgatccc atcctgttgt
    12601 cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg atgcctggcg
    12661 cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt tgggtgctgg
    12721 gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc
    12781 tggcgttctt cggctcagcg cctgaactgg gcgctggttt ccgacaccgt cgagtacggc
    12841 gagtggcgca ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac cttctttcgc
    12901 aaagtgtctc aggcgctggc tggtttcttc cccggctgga tgctgacgca aattggctat
    12961 gtgccgaacg tcgcacaggc tgaccacacc attgaagggt tacgccagtt gatcttcatc
    13021 tacccaagcg cactggcggt agtcaccatt gtggcgatgg gttgcttcta cagcctgaac
    13081 gagaagatgt atgtccgcat tgtggaagag atagaagccc gtaaacgcac ggcgtaataa
    13141 taataaacga cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg
    13201 acattaaaac tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat
    13261 ttaatgctct gcatcggtac tctgtatctc ctcaaacttt ataccgatga attagggatg
    13321 cctgcttact atggcgggat tatctttctg gtcgcgaagt tttttaccgc gtttaccgat
    13381 atgctcaccg gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt
    13441 ccctttattt tatatgcagc agttccggcg gcgttaattg caacgctcca gtttatcgcc
    13501 accacctttt gtttgccggt taaaacgacg attgccacca cgctgtttat gatgtttgga
    13561 ctttcataca gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac
    13621 ccgaatgagc gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg
    13681 atttgtaccg tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat
    13741 gcctgtgcgg cacttatgtt ctccattggc ggctttattt ttatgatgct gtgctacaga
    13801 ggcgtcaaag agcattatgt ggacacagcg ccaaccggac ataaagccag tattctcaaa
    13861 tctttttgcg cgatatttcg taatccacca ttgctggttt tatgcattgc taacctgtgt
    13921 actctggcgg catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg
    13981 aatgatatta atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc
    14041 gtattactgg tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc
    14101 atggtgctat gggcggtggg tgatatactg aattatttct ggggaagtaa ctctttcact
    14161 ttcgtcatgt tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg
    14221 gcactggtac cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt
    14281 tctgtttata ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg
    14341 ccaggcatta tgctgacgca aattggttat gttcccaaca tcgcgcaaag cgatgcgaca
    14401 ttacagggtt tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca
    14461 ttgacgatgg gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa
    14521 atcaaccaac gcaaaaataa agagatggcg acagaaaaaa aaacggcttc cgtaacgtta
    14581 taaatgtcaa tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa
    14641 tggacttatt atgaaaaaga ttaatgcaat aattttatta tcttctctga cctctgcctc
    14701 ggtatttgct ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg
    14761 cgaggttatg ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa
    14821 tacctacacc tttcagcgag aagatgaact aaaacatggc tataatgaaa ttgaaggttg
    14881 gtatccttta ttcaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga
    14941 taagagtatc agttcgggtg gtgcagtgta tctggacgtc ggtaatgact ccaacttact
    15001 gatagtgttt tatgttcaga taatgcccga tgaccttgtc atgcagctcc accgattttg
    15061 agaacgacag tgacttcc
//
LOCUS       DAJTPG010000093        14471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTPG010000093 DAJTPG010000000
VERSION     DAJTPG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(115..381)
                     /locus_tag="OO230_003071"
     CDS             complement(115..381)
                     /locus_tag="OO230_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5692740.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="OO230_003072"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="OO230_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5692741.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="OO230_003073"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="OO230_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="OO230_003074"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="OO230_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5692742.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="OO230_003075"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="OO230_003075"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5692743.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="OO230_003076"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="OO230_003076"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5692744.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="OO230_003077"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="OO230_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5692745.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="OO230_003078"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="OO230_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="OO230_003079"
                     /pseudo
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="OO230_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12643..14097)
                     /locus_tag="OO230_003080"
     CDS             complement(12643..14097)
                     /locus_tag="OO230_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5692746.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14471)
                     /locus_tag="OO230_003081"
     CDS             complement(14094..>14471)
                     /locus_tag="OO230_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5692747.1"
                     /translation="DGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGM
                     KPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAF
                     DDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3858 a         3648 c         3415 g         3550 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tacaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat c
//
LOCUS       DAJTPG010000094        14421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTPG010000094 DAJTPG010000000
VERSION     DAJTPG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..132)
                     /locus_tag="OO230_003082"
     CDS             complement(<1..132)
                     /locus_tag="OO230_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5692748.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="OO230_003083"
     CDS             315..458
                     /locus_tag="OO230_003083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692749.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO230_003084"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO230_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5692750.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO230_003085"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO230_003085"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5692751.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO230_003086"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO230_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5692752.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO230_003087"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO230_003087"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5692753.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="OO230_003088"
     CDS             complement(7051..7659)
                     /locus_tag="OO230_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5692754.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="OO230_003089"
     CDS             complement(7730..8815)
                     /locus_tag="OO230_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5692755.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="OO230_003090"
     CDS             complement(8818..9180)
                     /locus_tag="OO230_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5692756.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="OO230_003091"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="OO230_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5692757.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="OO230_003092"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="OO230_003092"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5692758.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="OO230_003093"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="OO230_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5692759.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="OO230_003094"
     CDS             complement(13472..13681)
                     /locus_tag="OO230_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692760.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="OO230_003095"
     CDS             13965..14327
                     /locus_tag="OO230_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5692761.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3523 a         3842 c         3624 g         3432 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaattac c
//
LOCUS       DAJTPG010000095        14211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTPG010000095 DAJTPG010000000
VERSION     DAJTPG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..141)
                     /locus_tag="OO230_003096"
     CDS             complement(<1..141)
                     /locus_tag="OO230_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA esterase"
                     /protein_id="HCR5692762.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAK"
     gene            complement(246..785)
                     /locus_tag="OO230_003097"
     CDS             complement(246..785)
                     /locus_tag="OO230_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5692763.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(815..1558)
                     /locus_tag="OO230_003098"
     CDS             complement(815..1558)
                     /locus_tag="OO230_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5692764.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(1586..1675)
                     /locus_tag="OO230_003099"
                     /pseudo
     CDS             complement(1586..1675)
                     /locus_tag="OO230_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1915..2553
                     /gene="ompW"
                     /locus_tag="OO230_003100"
     CDS             1915..2553
                     /gene="ompW"
                     /locus_tag="OO230_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5692765.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(2613..3119)
                     /locus_tag="OO230_003101"
     CDS             complement(2613..3119)
                     /locus_tag="OO230_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5692766.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(3165..3665)
                     /locus_tag="OO230_003102"
     CDS             complement(3165..3665)
                     /locus_tag="OO230_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5692767.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(3751..3930)
                     /locus_tag="OO230_003103"
     CDS             complement(3751..3930)
                     /locus_tag="OO230_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5692768.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(4310..5116)
                     /gene="trpA"
                     /locus_tag="OO230_003104"
     CDS             complement(4310..5116)
                     /gene="trpA"
                     /locus_tag="OO230_003104"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5692769.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(5116..6309)
                     /gene="trpB"
                     /locus_tag="OO230_003105"
     CDS             complement(5116..6309)
                     /gene="trpB"
                     /locus_tag="OO230_003105"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5692770.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(6321..7682)
                     /gene="trpCF"
                     /locus_tag="OO230_003106"
     CDS             complement(6321..7682)
                     /gene="trpCF"
                     /locus_tag="OO230_003106"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5692771.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(7683..9278)
                     /gene="trpD"
                     /locus_tag="OO230_003107"
     CDS             complement(7683..9278)
                     /gene="trpD"
                     /locus_tag="OO230_003107"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5692772.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(9278..10840)
                     /gene="trpE"
                     /locus_tag="OO230_003108"
     CDS             complement(9278..10840)
                     /gene="trpE"
                     /locus_tag="OO230_003108"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5692773.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(10862..10955)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(10932..10976)
                     /gene="trpL"
                     /locus_tag="OO230_003109"
     CDS             complement(10932..10976)
                     /gene="trpL"
                     /locus_tag="OO230_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5692774.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            11114..11995
                     /gene="yciV"
                     /locus_tag="OO230_003110"
     CDS             11114..11995
                     /gene="yciV"
                     /locus_tag="OO230_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5692775.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11992..12612
                     /locus_tag="OO230_003111"
     CDS             11992..12612
                     /locus_tag="OO230_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5692776.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            12713..13585
                     /gene="rluB"
                     /locus_tag="OO230_003112"
     CDS             12713..13585
                     /gene="rluB"
                     /locus_tag="OO230_003112"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5692777.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(13625..>14211)
                     /gene="cobO"
                     /locus_tag="OO230_003113"
     CDS             complement(13625..>14211)
                     /gene="cobO"
                     /locus_tag="OO230_003113"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5692778.1"
                     /translation="DERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTAAF
                     GTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDTAA
                     CREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITGRG
                     CHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
BASE COUNT         3500 a         3590 c         3619 g         3502 t
ORIGIN      
        1 ttttgccaga atagcgccgc caatatccat ttgtgacatt aaccaaccac caaagatgtc
       61 accattggca ttggtatcgg cgggcatggc taaagtacgt aaaacaagat cgccctgagg
      121 gacgttatgt gttgtagaca tggtaaaacc gacttaaaag gaaaatcaca ggcgcgatgc
      181 tactatgatt ttcctgagga gaacagagga gatacagcgc cagccccgaa ggactggcag
      241 tctggttagg atttatcttc ctgcggcatg tggcggtaga tatagatacc gcttaacaat
      301 gtgaagatta aggtaagggc ggtcaggcca aagactttaa agttgaccca aatattttgc
      361 ggcagccaga atgcgatgta gatgtttgcc agaccgcaaa ggataaagaa aacagcccag
      421 gccagattta gcttcgacca taccggttgc ggcagcgtga gttctttact cagcatccgc
      481 tgaattagcg gctttttcat cacccattgg ctgactaaca gggctcccgc aaacagagca
      541 taaatgactg taaccttcca tttaataaac tcatcattgt ggaagaacag cgtcaagcca
      601 ccgaagacga ccaccagaac aaaagtgatc agggccatct tctcaacctt acgaaagcga
      661 acccagctat agataagcac aatcgccgtg gcgacgatca gcgctgcagt agccgcatag
      721 atgtcataaa tcttgtaaaa cgcgaaaaag acaaccagcg gtaaaaaatc aagaaactgc
      781 ttcattttac gattccgtaa tcaaatgctt aaggttattg gcgaatcaac atatacaggc
      841 ggaacagata gatgagcaat atggcagaaa tcaggttgct caaggtgttc gccagtacgg
      901 caccaatttc cggggttaat gcggcaaaag aagaggcaaa aagcagcaac agtgtttttg
      961 ccagcaacca gctcagtact gcgggtgcca ccagacgcat attcgcccaa gtcagccgca
     1021 tactgctacg catcgaggca aaaatgccca ttttgtcctg taccagcatc accggtgcca
     1081 gggccagtaa aatggccatg ataattcccg gaacgaccac cagcatgatg ccaatctgta
     1141 ctaaaagggt agtcagaaaa atcagaataa ataacttggg caatatcggc gcactggcac
     1201 caatagctcg cagcgcactg actctctgac ccgcagacac cagctggata attaatatta
     1261 cgcctccggc gagaatggcg ttaccgatta attctgaaaa agtggacgcc gctgaagcct
     1321 gcagcaaaat ttgttgctgt tccggtgaca tattctgaac caggtcgaac aacccactac
     1381 tgccgctaac gggcacgccg tcattgagct gcgcaagctg tgcatcactg ggtgagaaaa
     1441 catgccctaa caccactgtg ataaacgcac atagcaacga taccaacaga atggtcataa
     1501 attgattacg gaagaaattt ccggtgtcac ggtatacgga ctgcgccgtg atagacatgc
     1561 actctccttg agttttatgc aggtgttaat tagcgggcaa ttgtaccctg gttaagcctc
     1621 gggtggcagc atcaggtctt gtatggaaaa gcatatcttt gtaaagcgga ggtaattgct
     1681 ggccttcagg cgtgggcaaa gcgcggcaag tggcgatcga gttaatattt gcgtagagaa
     1741 aatatttaaa aattgattta aatcacatta accaggattc tcaatgcaac ttctaaatta
     1801 atccagatca ataaatggtg aattatcata tgtaatgtga tctatgtagg atcatttgtt
     1861 actccaatgt aggtatattc gtcacgtttt tataaccata acgacggagc ggatatgaaa
     1921 aagttaacag tggcggcttt ggcagtaaca actcttcttt ctggcagtgc ctttgcgcat
     1981 gaagcaggcg aattttttat gcgtgcaggt tctgcaaccg tacgtccaac agaaggtgct
     2041 ggtggtacgt taggaagtct gggtggattc agcgtgacca ataacactca actgggcttg
     2101 acgtttactt atatggcgac cgacaacatt ggtgtggaat tactggcagc gacgccgttc
     2161 cgccataaaa tcggcacccg ggcgaccggc gatattgcaa ccgttcacca tctgccgcca
     2221 acactgatgg cgcagtggta ttttggtgat gccagcagca aattccgtcc ttacgttggg
     2281 gccggtatta actacaccac cttctttgat aatggattta acgatcatgg caaagaggcg
     2341 gggctttccg atctcagtct gaaagattcc tggggagctg ccgggcaggt gggggttgat
     2401 tatctgatta accgtgactg gttggttaac atgtcagtgt ggtacatgga tatcgatacc
     2461 accgccaatt ataagctagg cggtgcacag caacacgata gcgtacgcct cgatccgtgg
     2521 gtgtttatgt tctcagcagg atatcgtttt taattccgca caaaaacgac cccgtaatat
     2581 acggggtcaa taaggacatg gtataaaggg tattatttct tcgcttctac gccatcagtt
     2641 tcagaacgaa ttaaaaattt ctcagttgtt tgcggaatat gctgaatcag ccagtcggcc
     2701 atttgctttt cctcatttaa aatcgcttcg atgatcggaa ttgagactgt atcaccggca
     2761 ttttttgctg ctgctaatag tgaggtgtaa caggtgattt caaattgctc gaagacatat
     2821 ccgccaatag agcctttaac tatttcatca gaagggaata taccaccgat tgactgccca
     2881 agcgcagcca ttttactcat ggaatcttta atgactgaac gtgaaatgtc attacgatca
     2941 agaatagttt ccagttgaac aatctggttt ttggtttcac taagatgttg ttcaatacga
     3001 gcgcgtagtt caggataatt atctatacgg ctggccatgg attcaagcat agattcggct
     3061 tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat aatgttcaat acgattcatg
     3121 aaaatgtcct ctttataaaa tgactaaaag ttaaaattca tatttcaggc tttattttcg
     3181 gctttcttat ttacgttatt aagggccaga tcagtcagtt tgatgtcggt ggccttttct
     3241 tcttccaggg tttctttcag aagcttcgct gctttacggt aacctaattg ttcagccagc
     3301 gtcgctaatg tcccgtaact ggcaatctca taatgctcga ctttctgtgc tgcggcaatc
     3361 agtgcggcat cacgcacttc gtttttctcg gtactttcga tgacctcatt agcttcttca
     3421 ataagacctt ccattgccac acatttcatg cgcttaattt tcagatttga ttctgattcc
     3481 acaacttgat caattcgttc aatctgtcca tgagtttcct cgaggtgcgc atgaaaagcc
     3541 tgacttaatt tttcatttga tgttgctctt gcgagttttg ccagtgcccg ggttaattgt
     3601 ttttctgcgc tgtaggtatc tgaaagcagt tgaataaata catcttcaat ggtcttcata
     3661 ttcatatttt tctccagtga aatcactgcg agccatacgg cccgcagatg caattatctt
     3721 tatcagctga tattaataat taacggtgaa tcaggatttg cctgatttat taccaccgct
     3781 ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc ggatcatttt taaaattacc
     3841 gccgctatgc tgaccgcctt tacggcctgc gtcggatgcc ttctcacggt cttcggcgaa
     3901 atttcctgaa ctaccacgat gttcggccat gttatttctc ccgttgcgtt gcattgtttc
     3961 attaatatga gtgttgtgtg tcgacactca ttaaaattag tcactaatga gaattagtca
     4021 aattaagcgc aacgagaaga tagagggaaa tatattttga ggaacattct ggatatatta
     4081 acaattacct gaggaatatg tgacttagag aaaattattg attttactgg tgttatgttg
     4141 cgggattaat ttgtttgatt atattactgt tgggcggaaa aatgacgtaa gttgacgttc
     4201 gaccggggta agcgaaacgg taaaaagata aatattaaat gaatttagga tttttccggc
     4261 ttcattaaag aaagttaaaa tgccgccagc ggaactggcg gctgtgggat taactgcgcg
     4321 tcgccgcttt catcggttgt acaaaaactt tcagtgccgc cagcattttc tctggctcat
     4381 taatatgttg ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc gcagctcctg
     4441 catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaacccctgc aatggaggtg
     4501 cagcgttgta ctctttcagc ttcgcaacca gatgattgag gggtaacgcg gcgcggtttt
     4561 ctgcgccggt cacgcccgct cgtgacagca aatatgtgta accacgaccg taagaggcta
     4621 tctggcgcag caggtcgtca tcggcatttg gcgggcagat gaagataggt gcgacattat
     4681 ggcgcaacgc ggcctggcgg aagggcgcgg actcttcaac tggcacatcg gcaaccagca
     4741 ccgaatcgac gccgactttc tcgcactggg cataaaactc atcaatgcct ttgttaaaca
     4801 ccagattggc atacatcaac aggccaatgg gaatggtcgg gtgtttctgg cgaatcagtg
     4861 ccagcatttc aaaacattgt gccggagtca cacctgccgc aaaggcgcgc agagtggcgt
     4921 tttgaatggt cgggccatcc gccagtgggt cggagaaggg gatacctaac tccagcgcgt
     4981 cagcaccggc ttcaattagc gtatcgataa ttttcaatga ctgctcaatg cccggatcac
     5041 cgagcgtgac gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag
     5101 attcgtagcg ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat
     5161 gtctttatcg ccgcgaccgg aaaggttaac caccagtagc tgctctttat ccgggttttc
     5221 gcgcatcatt ttcagcgcat gggccagggc gtgggaggat tccagcgccg ggatgatccc
     5281 ttcgtgcagg cacagcgttt tgaaggcttc aagggcttca tcatcggtaa tagacacgta
     5341 atcagcgcgt ccagtgctgt taagatacgc gtgttgtgga ccgacagacg ggaaatccag
     5401 tccggcggag atggagtaag attcttcaat ctgcccgtct tcggtttgca tcatcggcgc
     5461 tttcataccg aaataaatgc ccacgcgacc atgttttagc ggtgcgccgt gctcgccagt
     5521 ttcgataccg tgaccaccag gctccacacc aatcaggccg acgttggttt cattgatgaa
     5581 atcagcaaac atgccgatgg cattcgaacc gccgccaaca caggcgataa cggcatccgg
     5641 caggcgacct tctctttcca gaatctgcgc tttggtttct tcgccaatca tccgctgaaa
     5701 ctcacggaca atggtcggat aaggatgcgg gccagccgcg gtgcccagca tatagtgcgc
     5761 ggtttcataa ctgccggacc agtcgcgtag cgcctcatta caggcatctt tcagcgtggc
     5821 ggaaccgcta tgtaccggga tcacttccgc acccattaag cgcatacgaa aaacgttagg
     5881 cgactggcgt tcaacgtctt tggcacccat ataaatacgg catttcaggc cgagcagggc
     5941 gctggcaagg gccgacgcca cgccatgctg accggccccg gtttcggcga tgatttcggt
     6001 tttacccatc cgcttcgcca gcaacgcctg ccccagcacc tggttggttt tatgtgcgcc
     6061 gccgtgcagc aaatcttcac gcttgagata cagcgtggtg ttcgtcccgg ctgtaatgtt
     6121 ctggcatttg gtcagtgcgg ttggacgccc ggcatagttt ttcagcaggt cgttgaactg
     6181 ggcttgaaat tcaggatctt tttgcgcgct gacaaaagct tcttccagct ggcgcagagc
     6241 aggcatcagg atttgtggca cgtacatgcc gccaaactca ccaaaatagg ggttaagtaa
     6301 tgttgtcatt gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga
     6361 cgtgcgtctt tgatgcccgg ttgcgactct acagcagaat taaaatcaag tccggcgcag
     6421 ccggtttgtg ccgcttccac gcagttatct gcgcctaagc cccccgccag cagaacgttg
     6481 ccaagcgatt gaccatttaa tagtgaccag tcaaaacgtt gcccgcttcc accctggccg
     6541 ttgtctaaaa catatttatc aacgtgctga aactcgcggg cgggcagggt ttcaccgacg
     6601 cttaatgctt tccagatggc aacatgtgct ggcagagctt cacgcagcgt atcgatatac
     6661 agctgatctt cattgccatg cagttgcact gccgccagcg ataacacctt agctttgtcc
     6721 accacatcgg caatatcgtg attgcggaac acgccaacat actgcaacgg tgccgcagcc
     6781 atcacttcct gcgcctgttc aacgttgacg caacgcggtg atgtcgcaac aaaaatcaac
     6841 ccaccgtaaa tcgcgcccgc gtcataagct gctttagcat cttgcccacg cgtcaggcca
     6901 catactttat tctcacccag caacacccgg cgcacggcgg cgtgcaaatc gtcatgggcc
     6961 atcaacgccg aaccaatcag aaaaccgtta gcgaagtggc ttaactcgcg cacctgagcg
     7021 taagtattga tgccggattc gctgattacc gttacgttgt gccccagttt cggcgcaagc
     7081 tcgcgggtac ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt gatgccaacg
     7141 acctttgccc ctaatgcaat ggcgcgctcc agttcctctt cattactgac ttcggtcagc
     7201 acccccatct ccagactgtg agcgacggct gcaagctggc gatattggtc gtcatccagt
     7261 actgaaagca ttaataagca ggcatcggcc tggtaatagc gcgccagata gatctggtaa
     7321 gggtcgataa tgaagtcttt acataaaatc ggctgcgggg cgatttggct gacgatgagg
     7381 aggaaatcaa agctcccctg aaaatatttc tcatcagtca gcaccgaaat tgccgaagcg
     7441 taatgtttat aaatggcggc aatgcgtgcc ggatcgaaat catcacggat cacgcctttt
     7501 gacggcgacg cttttttaca ctccagaata aacgccgtgc gtgcaccctg tagcgcatca
     7561 taaaaatgtc gtgtgctcgg ctgaacctca ttctgaaaac tggccagcgg ttgctgctgt
     7621 ttgcgggttt ctacccaaat cgccttgtct gcgacgattt tcgctaaaac ggtttgcatc
     7681 atttaccctc gtgccgccag cgcggtaact ctgtcgtaag cggaaccact gcgcagtacc
     7741 tcaagaacgg tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg cattaacatg
     7801 gcgacgttcg cagcgacggc tgcttcatgg gcggcgtcgc ctttaccttg taacaagcgt
     7861 gttaaaatgt cacggttttc ttcaggtgtt ccgcctgcca gttgctcctg gtggtaggga
     7921 gtcaggccaa aatcttcagc ggtcaattga tagctcttaa ttttgccgtc atgcagctcg
     7981 gcaacgattg tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg caccaccgcc
     8041 gcgcgttgat accccagcac gcgcaaggtt tcggcaatcg gcagcaccag ttccggacta
     8101 taaacaccaa ttaacgccag cggcggatgc gccgggttaa tcaatggccc cagcacattg
     8161 aacagggtgc gggttttcag ttgctggcga accggcatcg cgtggcggaa tccggtgtga
     8221 tatttcggcg caaagaggaa acatacgcct aactcgtcca gcgcctggcg cgatttattg
     8281 gcgttcatat caagattaat accgaatgcc gccagcagat ccgacgagcc ggatttactg
     8341 gagacgctgc ggttaccgtg tttcgccact ttcagcccac aggccgcggc gacaaacgca
     8401 ctggcggtag aaatattgat actgttgctg ccgtcaccgc cagtaccgac gatatcagca
     8461 aacagataat ccgggcgcgg gaacggcgct gcgttttcca gtagcgcggt tgctgccccg
     8521 gcgatctcgt tcgggtgctc accgcgaatt ttcatgctca ccagcgccgc cgccagttgt
     8581 tccggcttca gctcgccacg caccaccgct gaaaacagct ggtggctttc ttgttggcta
     8641 agcgtctgcg cctgatacag tttttccaga atcggttgca gcgtgttggc tggctctagt
     8701 ttctgctgcg cccaggccag cgtttgttcc agcaggcgag cgccctgggt ggtgagaatg
     8761 gattccggat ggaactggaa tccacaaacg cgatccgcat cgtgacgtac tgccatcacc
     8821 atgccattaa aatgggcgtt gatggttaaa ccggccggaa tgttactgcc aaccagcgag
     8881 tgataacgcg ccaccggcag cgggtttgtt aatccggcaa acatcgcctg accgtcatgt
     8941 tcaatgctcg acgctttacc gtgaagaatt tcgcccgcct gaccgacata gcccccgtaa
     9001 gcttcgacaa ttgcctgatg tccgaggcaa atgccaataa tgggcagctt gccacgcaag
     9061 cgggtgagga gttccggcat acaaccggct tcgctcggca caccggggcc aggagaaagc
     9121 atcagtaccg gattgctcat cgtcgccagg cgttcaatta aggtctgcgc cggaatatgg
     9181 ttgcggtaaa tcaccacgtt atgaccattg ctgcgcaact gatctgccag gttgtacgta
     9241 aaagagtcga tattatcgag cagcagaatg tcagccatca gaaagtctcc tgtgcatgat
     9301 gcgcggtggc aatagcgcgc agtacagcgc gggctttgtt acgggtttcg tcggcttccg
     9361 actgcggaac agaatcaagg actacaccag cacccgcttg cacggtggcg ataccgtttt
     9421 ccaccagcgc cgagcggatc acaatgcagg tgtcgagatc gccatgcgcg gtgaaataac
     9481 ctaccgcgcc gccgtagctg ccgcggcgac gaccttccgc ctcggcaatt aactgcatag
     9541 cgcgtacttt cggcgcaccg cttaacgtcc ccatattcat acaggcgcga taagcgtgca
     9601 gggcgtcaag atcgtgacgc agttcgccga ctacgcgaga gacgaggtgc atcacatagg
     9661 aataacggtc aactttggtg agatcggcga tgtagcggct gccaggggtg caaatgcgtg
     9721 ccagatcatt acgggcgaga tcaaccagca tcagatgttc agacagctct ttatggtcag
     9781 tacgcatttc cagttcaata cggctgtcga gatctctgtc cagtgaacca tcggcgcgac
     9841 gaccgcgtgg gcgtgttccg gcaatcgggt agatctcaat ctggcggctg gtggcatcat
     9901 acttgagcga gctttccggc gacgcgccaa atagggtgaa atcattatcc tgcataaaaa
     9961 acatgtacgg gctgggatta ctctttttca gcacgtaata ggccgccagc ggtgacgggc
    10021 agggcagaga gaaacggcga gatggcacca cctggaaaat ttctccagcg cgaatcgctt
    10081 tttgcaacaa acgcactacg ccaccgaact cttcatcgct ctgattacat tcacaacgca
    10141 tatgcggcac ggaaaccact ggcagcggcg gcgcggcttc ggtcagttgc tgacgtagtt
    10201 cgttcaggcg agcagtgaga cgttgttttt cttcttcatt cggagcaaac aggctggcct
    10261 gaatacgggt gctttttttc tgatggtcaa tcaccatcag cgtttcagcg agataaaaac
    10321 agaaatcagg gcagttattt tccgctgaca gttgcggtaa atcttcaaat cccgccacaa
    10381 ggtcataaga gaacaggccg ccgaagaaca ttgcttctcg ttcttccttc ggtacattca
    10441 acagattctg caataaacgg aaagcgtcaa aaaccgaaag ggagcataag cgggcgtctt
    10501 catccagcag tggactgaca ggggggaagc gcagcacacg gcagtttggt gattgttcac
    10561 tttccacacc cgcaggcagg gcgttatcca gtagtgccag cagggcttcg ccattgccgg
    10621 aaagtgcctg aattgtgacg gtgtcaccta atgctgtaat gcgcagcgca ctgtctatca
    10681 gcagcaggct ttttaaatca tctttgctgt cgatatctgc ggattccagc agcagcgttg
    10741 ccggacgatt cccacacaac tggtgaaaaa gcgcagtcgg gttgtcgcga taagcgcctt
    10801 cgcaggttag cagttcgaga gtcggttttt gtgtttgcat tgttattctc taattttgtt
    10861 caaaaaaaag cccgctcatt aggcgggctg ggtatctgat tgctttacgc atggtgaata
    10921 cactgcccgt ttcaggaagt gcgccaccaa cctttcagta cgaaaattgc tttcattgtc
    10981 gatacccttt ttacgtgaac ttgcgtacta gttaactggt tcgatgatta attgtcaaca
    11041 gctcatttca gaatatttgc cagacccgtt atgatgtcgg cgcaaaaaac attatccaga
    11101 acgggagtgc gccttgagcg acacgaatta tgcagtgatt tacgacctgc acagccatac
    11161 cacagcttcc gatggctgcc tgacgccaga agcattggtg caccgtgcag tcgagatgcg
    11221 cgtcggcacc ctggcgatca ccgaccatga caccacagct gcaatcgcgc cagcaagaga
    11281 ggaaatttca cgttcgggac tggcgctgaa tcttattccc ggcgtggaaa tttccacggt
    11341 ctgggaaaat catgaaattc atattgtcgg gctgaatatt gatattactc atccgctaat
    11401 gtgtgagttc ctcgcgcagc agacagaacg gcgcaatcag cgggcgcagc aaattgccga
    11461 aagacttgaa aaagcgcaaa tacctggggc gctggaaggc gcacaacgac tggcgcaggg
    11521 cggcgcagtg acgcgcggtc attttgcgcg tttccttgtg gagtgcggca aagccagttc
    11581 aatggcagat gtctttaaaa agtatctggc gcgcgggaaa accggatacg ttccgccaca
    11641 gtggtgtaca atagaacaag ctattgatgt cattcatcat tctggcggta aggcggtgct
    11701 agctcatcct gggcggtaca atcttagtgc taaatggctg aaaagattgg ttgcgcattt
    11761 tgccgaacac cacggcgacg cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg
    11821 cacccagctg gccacacttg cgcgtcagca tcatttatgg gcatcacaag gatctgattt
    11881 tcatcagcca tgcccgtgga tcgaactggg tcgtaaactc tggctgcccg caggcgttga
    11941 aggcgtctgg cagctatggg aacagccgca gaacaccaca gagagggaat tatgagccag
    12001 tttttttata ttcatcctga taacccgcag caacgtctga tcaaccaggc ggtggagatc
    12061 gtgcgtaaag gcggggtgat tgtctatcca actgattccg gctatgcgct cggctgtaaa
    12121 attgaagata aaaacgccat ggagcgtatt tgtcgtattc gccagctgcc ggacggtcac
    12181 aactttaccc tgatgtgtcg cgatctttct gaactctcga cctattcatt cgtggataac
    12241 gttgcgtttc gtttgatgaa aaacaacacg ccgggcaact ataccttcat cctgaagggg
    12301 acgaaggaag tgccacgccg tctgttgcag gagaaacgca aaaccatcgg tatgcgtgtg
    12361 ccttctaacc cgattgcgca ggcgttgctt gaagcactgg gcgaaccgat gctttccact
    12421 tcgctaatgc tgccaggcag cgaatttacc gaatccgatc ccgaagaaat taaagaccgt
    12481 ctggaaaaac aggtagattt gattattcac ggcggctatc tcgggcagaa accaacaacg
    12541 gttattgatc ttaccgatga tacgccggtc gtggtgcgtg aaggcgtagg tgatgtgaag
    12601 cctttcttat aagtgcgtaa ctctgtatac tacgcggcct taaaatggcg ctatacggcg
    12661 ccattgattc tcctgttcga cgcctgtgaa ggcgacacca aaaggaagct ctatgagcga
    12721 aaaattacag aaagtgctgg cccgcgctgg ccacggctcc cgccgtgaaa tcgaatctat
    12781 tattgaagcc ggtcgtgtga gtgttgatgg caaaattgct aaactcggcg atcgcgttga
    12841 agttacccct gggctgaaaa ttcgtatcga tggtcatctg atttcggtac gtgagtccgc
    12901 tgaacaaatt tgtcgcgttc tggcctatta caaaccggaa ggtgagttgt gtacccgtaa
    12961 cgatccggaa gggcgtccga cggtgtttga ccgtctgcca aagctgcgtg gcgcacgctg
    13021 gattgccgta ggtcgtctgg acgttaacac ctgtggtctg ctgctgttca ccaccgatgg
    13081 tgaactggca aaccgtttaa tgcatccaag ccgtgaagtt gaacgtgaat acgccgtgcg
    13141 tgtatttggt caggttgacg acgcgaaact gcgtgatttg agtcgtggcg tgcaactgga
    13201 agatggtccg gcagctttta aaaccatcaa gttcagcggc ggcgaaggga tcaaccagtg
    13261 gtacaacgtg actctgaccg aaggccgtaa ccgtgaagtt cgtcgtctgt gggaagcggt
    13321 tggtgtgcag gtgagccgcc tgatccgtgt tcgttacggt gatatcccac tgccaaaagg
    13381 tctgccacgc ggtggctgga cggagctgga cctcgcccag actaactatc tgcgcgaact
    13441 ggtggagctt ccgccagaaa ccagctctaa agtcgctgta gaaaaagacc gtcgtcgcat
    13501 gaaggctaat cagattcgtc gtgcggtgaa acgtcacagt caggtgagcg gcagtcgtcg
    13561 ttctggcggg cgtaataacg gttaatcaaa gtatgccgga cgtcatatcc ggcattttta
    13621 cagattaata atcaatccct atttgcgcct taacaccggc atcaaacgca tgtctgatgg
    13681 ggcgcagctc acttaccgta tccgccagtt caagaatatc ccgatgacag cctcgacccg
    13741 tgataatcac cgtctgttga tgtggacgtt catttaacgc ctgcaccact tcttccagtg
    13801 gcaaatagtc atacgccacc atatacgtca gttcatcaag caaaaccata tccagtgagg
    13861 aatcagcaag catccgcttt gcatgttgcc agacttcgcg gcaggcggcg gtatcagact
    13921 cgcggttttg tgtatcccag gtaaagcccg ttgccatcac ctgaaactca acgccatgtg
    13981 gctccagcag attgcgttcg ccattaggcc aggtgccttt aataaactgc acgacgccca
    14041 ctttttttcc gtgaccaact gcgcgtgttg ccgtaccaaa tgccgcggtg gttttgcctt
    14101 ttccattgcc ggtaaagacg atgataatac cgcgttcatc ctgggcctgg gccacacgag
    14161 catctacttt ttctttcact cgctgctgac gctgttggta gcgttcatca c
//
LOCUS       DAJTPG010000096        14146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTPG010000096 DAJTPG010000000
VERSION     DAJTPG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..2861
                     /locus_tag="OO230_003114"
                     /pseudo
     CDS             1..2861
                     /locus_tag="OO230_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2871..6517
                     /locus_tag="OO230_003115"
                     /pseudo
     CDS             2871..6517
                     /locus_tag="OO230_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6578..6985
                     /locus_tag="OO230_003116"
     CDS             6578..6985
                     /locus_tag="OO230_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692779.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7527..8615
                     /locus_tag="OO230_003117"
     CDS             7527..8615
                     /locus_tag="OO230_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5692780.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8626..10905
                     /locus_tag="OO230_003118"
     CDS             8626..10905
                     /locus_tag="OO230_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692781.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10898..12035
                     /locus_tag="OO230_003119"
                     /pseudo
     CDS             10898..12035
                     /locus_tag="OO230_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            12032..14062
                     /locus_tag="OO230_003120"
     CDS             12032..14062
                     /locus_tag="OO230_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692782.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     EDNLLTLSLPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKT
                     RAWALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQA
                     ELLANDLTAVGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3489 a         3724 c         3624 g         3309 t
ORIGIN      
        1 tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc atctactggg
       61 gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg agcacattct
      121 tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact attgtggtgc
      181 gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc gatttgctcc
      241 ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc cgttcgcatt
      301 aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa tgacgaaagc
      361 ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag tgcaaaaaga
      421 agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag cactggcaga
      481 acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat gccagcacca
      541 actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag tagtctcccc
      601 accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag cgccattagg
      661 catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg cgaaatatat
      721 ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc tgttggcaag
      781 gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag cggtagttga
      841 ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt ttcattcacc
      901 taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg ctggagaaag
      961 cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa tacgccatgt
     1021 tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc tatccacagg
     1081 aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc gtcgcccgtg
     1141 ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg aaatacccgg
     1201 aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa gcacaggata
     1261 acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta ctgcaagaaa
     1321 tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg cttgcgctcg
     1381 atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat cagccatcga
     1441 tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac gccctcctcc
     1501 acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga ttattgcagg
     1561 tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg tttaccgcct
     1621 ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta ggcgttctcg
     1681 gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct ggtgaatccc
     1741 agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt gatatcgccc
     1801 tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag gagcgggcaa
     1861 aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag cttgaagatc
     1921 ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat tttggcccac
     1981 gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat gtttccggca
     2041 gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag gcgaacgatg
     2101 ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc cagcaggtag
     2161 caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac ttccaactct
     2221 tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg ggcgtttata
     2281 gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc tacagcaccg
     2341 ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt cctcacgttc
     2401 tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac tacgaactgc
     2461 taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc gagcgcaacg
     2521 ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc atggggctgg
     2581 caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc aaacctctcg
     2641 gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata tcaccagccg
     2701 aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa gcggaaagct
     2761 atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat gccatcaccg
     2821 ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc atggacaagg
     2881 aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa ggcaaaacac
     2941 cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa agcatcattc
     3001 agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt gtctggcgtt
     3061 atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac ttgatcggcg
     3121 aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc gcgcaaaatg
     3181 acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat gcgactgagg
     3241 tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg gtagtcacat
     3301 tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat aacgaattcg
     3361 atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag cgttgcgccg
     3421 acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt attgcgctga
     3481 tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc ccgcggactc
     3541 attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg gtgagaaaac
     3601 tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac atgtcgcatg
     3661 aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga ctggcagtcg
     3721 ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg gtacaaatta
     3781 accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa cccagcctgc
     3841 aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca ctggcagaac
     3901 tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa gacaaaaagc
     3961 tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa acgctgatgg
     4021 catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa gcccgggcag
     4081 tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc gataacagcc
     4141 aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag acttccatac
     4201 gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg ccgaataaac
     4261 actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct tttgacgaac
     4321 gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac gttcatagcc
     4381 tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct cagcaactgc
     4441 aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc aaatctaaac
     4501 agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg ttgatgggag
     4561 ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg gggatttgga
     4621 atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg caacaaattg
     4681 tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg ggtaccgatg
     4741 ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt ccgctgctga
     4801 ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac ctttttgcgg
     4861 gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg gctactgcac
     4921 ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta cttgccctgc
     4981 gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat cgttggcagc
     5041 gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata gagatttacc
     5101 cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggccc aggccgcgcc
     5161 ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc gaaatgctgc
     5221 gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg ttctgccagc
     5281 cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa gctggtgccc
     5341 ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa agcacggcac
     5401 gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc cgtgcggtca
     5461 gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag ctgcatcata
     5521 tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt cttcaggtag
     5581 tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc ccaacactgg
     5641 gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt accgttcgct
     5701 tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag aaaagtgttc
     5761 cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc cgactaaaag
     5821 ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt gaagctgccc
     5881 tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt aatcatccct
     5941 ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg aaccgcgtcg
     6001 tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc aagatgagcc
     6061 aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa tgacagcaga
     6121 aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc cttttcgcca
     6181 gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca gcctgactcg
     6241 ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca aaggctggga
     6301 gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg tccttaagtt
     6361 ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct tccgttctct
     6421 tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt ttgatttgag
     6481 tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca gtgatgcgac
     6541 tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa aagagctggt
     6601 tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca gacttgagat
     6661 ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt tccggctacg
     6721 tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg atgcgttgat
     6781 ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac ctgaacctgt
     6841 aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag aattatatac
     6901 tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata gctttattaa
     6961 aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt caacaaatca
     7021 atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt taacttttgt
     7081 gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct tacccttaca
     7141 ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg gatcagagca
     7201 ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat ccggcgcaac
     7261 ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc cagaggtggt
     7321 tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc tgaaaatagt
     7381 aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg ctttccagat
     7441 atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata tcgtttattt
     7501 cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg cagcgtccgc
     7561 ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat aacgcacctt
     7621 gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt ggcgatagcg
     7681 cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt atgttagtca
     7741 ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca aaatctctcc
     7801 tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc cagggcggcg
     7861 catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt atcaatcatg
     7921 gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc gacggcaaaa
     7981 tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt ctgttaggta
     8041 tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt cagctctatg
     8101 cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc gtcaatgaaa
     8161 tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt atggatttcg
     8221 cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat agcggtattc
     8281 cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc gatctgcgtg
     8341 ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc accgctgaag
     8401 ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat cgcgcaggtg
     8461 agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac gaagaagatc
     8521 gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa gctcactggc
     8581 aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag cgagccgtta
     8641 attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc gttaatcgaa
     8701 agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga ccgggtagac
     8761 gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca gtatcaggac
     8821 ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc ggagtggcag
     8881 gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt gccttgctgg
     8941 gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca gaccttgctg
     9001 ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt cgaagatgaa
     9061 agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact gcggggcgac
     9121 tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg gattggatgg
     9181 gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca cgctccggca
     9241 ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt gccgtcgctg
     9301 gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct tgatgtgctg
     9361 gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg gcagtggggc
     9421 ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg ccagcacaag
     9481 ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc actggcacag
     9541 ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc aggatcgctg
     9601 attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat tcatcccgat
     9661 accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt cggtaaactt
     9721 gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact ggaacgtacc
     9781 gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg actagcgcaa
     9841 agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg ccagcaggga
     9901 agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg cccgcttagc
     9961 cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga agccgcacgc
    10021 cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac cacatgtctt
    10081 agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga gcaactcaca
    10141 ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga agtgctatat
    10201 gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt acaaacgacg
    10261 ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag accggatgaa
    10321 aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct gcgcgatcta
    10381 catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt gctggagcga
    10441 cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg tgcgctgatg
    10501 cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc gcagttatcc
    10561 ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca tcaactggcc
    10621 tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag tgatgccgat
    10681 tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc acgagaacgc
    10741 gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc cgtttcggca
    10801 ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca actcgaacag
    10861 caggcactgg catcgctgca acactgggga gttttccatg tctgaactga acgatcttct
    10921 gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag aaacgacgct
    10981 ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc tgtatgggcg
    11041 cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct caaatctcac
    11101 cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga ttgagcggct
    11161 ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc tcgacgtgct
    11221 ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca aacatctgat
    11281 gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg aagaaattat
    11341 ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc gtcgtcgccg
    11401 ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg ccaacctgca
    11461 acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta acagccgcat
    11521 taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg gatcgatggt
    11581 cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac ccggcattcg
    11641 tacccatctg gtggcgtttg accacaagcg tcgttgatct cacggcagac gttgccgatc
    11701 cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc agtgccatgg
    11761 agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc gtgagcgatt
    11821 tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt gtccagagcg
    11881 gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc tatgaccgcg
    11941 atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca ccgggcgagc
    12001 tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg aattattaga
    12061 actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc gcagccttaa
    12121 ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt taatcgccac
    12181 cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag aggctcagtg
    12241 cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg
    12301 actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg caatctggct
    12361 ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc tggtcgctaa
    12421 aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc gcttaccgat
    12481 gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg attgtattga
    12541 aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg caaaagcgca
    12601 gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca catcatctga
    12661 cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac aactaagcca
    12721 ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg ctttcagccg
    12781 cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc tacggcaact
    12841 gcgcgccagc gttgatgcct tccacgcccg cgccagcgtt gatgccttcc acgcccgcgc
    12901 cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca gtcgattaaa
    12961 ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa gttcctcctg tgccgtggcg
    13021 cacggtcgtt ggctctggca ttgccggaga agcaaagctt gatcatctgc ggctggtgtc
    13081 tttaggtatg cgttgctggc aggatattga gcattatggt ttacgcatct ggtttaccga
    13141 tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg aacaggaaga
    13201 ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactgg cgggtgggca
    13261 aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag ctacccgcaa
    13321 ccgcttaagc agcgttgtac cgctgtcgcc tgatgcctgg caaatgttga gcgcgccgtt
    13381 acgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc ccgcctgcat
    13441 acggcctctt aatcaggtcg ataacttatt tattctgccg gtcgctgagt gtatttcgct
    13501 cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg aaggtgaaga
    13561 taatctgctg acgttatcac tgccagcctc agccagcgcg ccttatgcca ttgaacgcat
    13621 ggcggcgctt ttgcaacaaa cagacgaccc cgtgtgtctg gtttctggct ttgtcagttt
    13681 tgttgatggg caattgacac tggaaccaca ggtaatgatg acaaagactc gtgcctgggc
    13741 gctggacgca gaaactgcgc ctgtggtggt atcactacct tctgccagcg ttctgcctgt
    13801 gccgtctact gctcatcagt tgctgatgcg ctgccaggcg ttacttattc aactgctcca
    13861 taacggctgg cgctatcagg aacagagcgc tattaatcag gcagagttgc tggcgaatga
    13921 cctcaccgcg gtgggttttt atcggctggc acatgtgttg gcacaatttc gtaatacaga
    13981 aagcgaggca cgggtagaag caatgaataa cggtgttttg ctttgcgaac aattattccc
    14041 catgcttcag caacaaggat gaaatagtgc tttttactaa gagttctact ccagttccgg
    14101 actgctcacg ccacggtatt aggcatatcc tatatagccc ctggtg
//
LOCUS       DAJTPG010000097        13963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTPG010000097 DAJTPG010000000
VERSION     DAJTPG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="OO230_003121"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="OO230_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5692783.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="OO230_003122"
     CDS             2218..2766
                     /locus_tag="OO230_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5692784.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="OO230_003123"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="OO230_003123"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5692785.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO230_003124"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO230_003124"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5692786.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO230_003125"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO230_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5692787.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6727..8127)
                     /gene="asnS"
                     /locus_tag="OO230_003126"
     CDS             complement(6727..8127)
                     /gene="asnS"
                     /locus_tag="OO230_003126"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5692788.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="OO230_003127"
     CDS             complement(8296..9498)
                     /gene="pncB"
                     /locus_tag="OO230_003127"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5692789.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9764..12379
                     /gene="pepN"
                     /locus_tag="OO230_003128"
     CDS             9764..12379
                     /gene="pepN"
                     /locus_tag="OO230_003128"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5692790.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="OO230_003129"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="OO230_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5692791.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..>13963)
                     /gene="ssuC"
                     /locus_tag="OO230_003130"
     CDS             complement(13350..>13963)
                     /gene="ssuC"
                     /locus_tag="OO230_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR5692792.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3551 a         3563 c         3526 g         3323 t
ORIGIN      
        1 aacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgctggt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgtgacg tggtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccgatcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcgctttc aacgccgccg ctttcggtga ttaaccaatg gcagctagcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggtctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg tgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggagcagat
     1201 ggtgctattg gcccggcaac gcgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggcac tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccagccta ttcgctgctc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcacttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta ccaccaactc tggcacgcaa agatattctg
     1501 ccgaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg gaggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt ttcgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggatgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tgatcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcaa ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgag ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttccattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attacccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat cgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttgatctggt gtgaacaaac ccgcctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatct acacttggtt atgagcgcct gcacaatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa aggcgcataa aaaagtccac ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaac
     3661 gcttacagca ctgccacaat cgattcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaacagca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcagacc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcaccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaatgc atcgctgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggagggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acgatttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcgttcagac tgttaatcag tgcatcgaag
     4381 tcaagggtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacacacg cttaacgctg
     4501 gtattttttg ccaggaagtc ggcagccacg cgtagtgcgc cagtgccacc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcaag gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggc
     4981 ataaaaaaaa caggaccaac gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gttttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt tatgacacct gccactgccg tcaataagtt
     6181 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
     6241 catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt gctacaaaga taaagattta
     6301 gtttcaaaaa gaaaaaatat gtaaccaaaa gtaaaaatta aggagctttg tgaacaccgt
     6361 catatttcca tagaaacgtg atgatattta cagcaatttt tatctattta tatgatttac
     6421 ttatatttaa attaactaaa cggaaatttt gtttctgatg gaaactttat cgacctggca
     6481 caaaatcctc tttcagacat ccagaatgcc aaaaaatagt atgaaaattg tgctatcagc
     6541 taagaaaaaa ctaatccgca ataagagtac gggttatttt ttgtgcgtaa atgttgcaat
     6601 gtaatattcc gtaacaggat gatcgttatg gcgggggtgc aaatagattg cttgacttaa
     6661 caaacagata acttgacaga aaggataaaa aagagccagc aggcgctggc tctttgagga
     6721 agaaaatcag aagctggcgt tgcgcggagt acgtgggaac ggaatcacat cacgcacgtt
     6781 ttgcacgcca gttacgtaag caatcaggcg ttcaaaacca agaccgaaac ctgaatgcgg
     6841 aacagtaccg tagcgacgca gatcgcgata ccaccagtaa tcttctttat tcaggcccat
     6901 ttccagcata cgctcgtcca gaacgtccag acgttcttca cgctgggagc caccaatgat
     6961 ctcaccgatg cccggagcca gaacgtccat agccgcaacg gttttaccgt cttcgttgag
     7021 gcgcatatag aacgctttaa tatctttcgg atagttttta accactaccg gtgctttaaa
     7081 gtgttcttcc gccagataac gctcatgctc agaagagaga tcgacgcccc agtaaaccgg
     7141 gttttcaaac ttcctgccgc agttttcgag aatggtcact gcgtcggtgt aatccacctg
     7201 cgcgaaatcg gcttcaatga agcgttccag acgtgaaacg gcatctttat ctacgcgttc
     7261 agcgaagaat ttcatgtcgt ctgcgcgttc ttcgagaacc gctttgaaga catatttcag
     7321 catggcttca gccagacccg cgatatcgtt caggttagca aacgccactt ccggctccag
     7381 catccagaat tccgccaggt gacggctggt gttggagttt tcagcacgga aagtcgggcc
     7441 gaaggtataa attttggata atgcgcaagc gtaggtttcg ccgttcaact ggccagatac
     7501 ggtcaggaaa gactctttac caaagaagtc tttgtcgaaa tccactttgc cctgatcgtt
     7561 acgcggcagg ttttccagat ccagcgtaga aacgcggaac atttcgcctg caccttcggt
     7621 atcagatgcg gtaatcagtg gcgttgaaac ccagaagaat ccctgctcgt taaagaagcg
     7681 atgcagcgcc tgcgccagcg tatggcgaac gcgcgcgacg gcaccaatca ggtttgtgcg
     7741 cggacgcagg tgagcgactt cacgcagata ctcaatgctg tggcgttttg ccgccatcgg
     7801 gtaagtgtct ggatcttcaa cccaaccagc aacttcaacc ttgctggcct gaatttcaaa
     7861 ttgttgcccc tggcccggcg atgccacgac tttacccgtc acaatgaccg agcagccggt
     7921 ggtcagacgc aggacgtctt cattgtaatt gggcagagaa ttattgatga cagcctgtac
     7981 aggatcaaag caggaaccgt cataaacggc gaggaaggag atgccagctt ttgaatctcg
     8041 gcgggtacgt acccatccgc gtacggtgac ttcgctgtca acggctacgc ggccctggag
     8101 tacgtcggct acaggcacaa cgctcataat attctctctg ttaatagtcg gaaaaaataa
     8161 acacttgtcc acccgtaaat gggggggata cctatgttac ctggcatctg caatcagaca
     8221 agcagaattc gcaaatgcag cgaaagattt cggaattaaa ggtaaaaaag ggagccgatt
     8281 agctccctga tgatattaac tggctttttt aatatgcgga aggtcgaacg ctttgcgcag
     8341 cgcccgaaca aacgctttat catggcagat agttttgcca gggctgtcag aaagtttcgc
     8401 caccggttta ccgttacact ctaccaactt aatcacaata ttcaggggtt ttacctgggg
     8461 gatatcgcag gtcaggcgag tcccaatacc aaaacttaat tgcacgcggg aagagaagtg
     8521 gcggtatagc tcaaccgctt tgcgtaaatc cagattgtca gagaaaacca gcgttttact
     8581 ctgtggatca attcccagct tttcataatg tgcaatggct ttttcacccc attcaaccgg
     8641 gtcgccagag tcatgacgca ggccctgata ccgactagcg aactcgacac cgaaatcacg
     8701 caggaaagca tccatagtga tgcagtcggt taatgcaatg ccaagttggt cgggatactc
     8761 ttccagccag gcagcaagtg cagctcgctg gctgttggct agatccgggc tgatttgctg
     8821 atgtgcctgg aaccattcgt gtgcctgtgt tcccatcggc gtgagggaaa gccgacgcgc
     8881 cagatcgtag ttgctggtgc ccacgaacca ggattcctgt tgcagacgct taacgatggt
     8941 ttcttgtact tcgcgagaaa aacggcgacg ggtgccaaaa tccatcagat ggaagcgcga
     9001 catatcaaga ccggcggtta acgccgagaa gtcgactaat ttgctttcca gcgtgtcgag
     9061 ggcttgcgca acgtcggcct gcggtgagcg atagcgatgt accatttcac tgataaccgc
     9121 cagcaaagga acttcccaga ggatgacttc acgccacgga ccgcttaagc gaatatccag
     9181 cttgccatta tcgttggaca cggtgacttg ttccgggtta aagcggaact cgcgtaacca
     9241 gttaagatag tcggccttaa agaaaggcag ggcagagagc cactgatatt catcatcctg
     9301 caggcgcagg tgctgcatcg cctgaatctg ttcacgaata gcatcggcat aaatacccag
     9361 cagatcgtca cctcggcaac gaaactccgc cgcgacatgc acatcgtaat agtgatgaaa
     9421 cacggcttgc tgcatatgca acttataagc atctgtatcc agcaacgagt gcagaacagg
     9481 agaagcgaat tgtgtcatag gtgcgcagta gcgtcctcgt acaggagcgt ttagtacaat
     9541 aaacatcttc aggaactgct ggagtatacc ttgtttcgca atttattgaa ccccgatcac
     9601 accatatgcc acctttctgg tcgatggcat tacgcggtgt atgttataaa aatgtagcaa
     9661 taaaggcgtt tgtacctgaa aagatgaaga ttctgcatag cgcgatttac gcaacaggaa
     9721 tagactgaac accagactct ataaaagatg ctaaaggtta tttatgactc aacagccaca
     9781 agccaaatac cgtcacgatt atcgtgcgcc ggattaccag attactgata ttgacttgac
     9841 ctttgacctc gacgcgcaaa agacggtcgt taccgcggtc agccaggctg tccgtcatgg
     9901 tgcatcagat gctccgcttc gtctcaacgg cgaagacctc aaactggttt ctgttcatat
     9961 taatgatgag ccgtggaccg cctggaaaga agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cgttttacgc ttaagatcat taatgaaata agcctggcgg cgaatacggc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaatcaaat atcccttcct gctttccaat ggtaaccgcg ttgcgcaagg
    10261 cgaactcgaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gataccttta ccacgcgttc
    10381 tggtcgcgaa gtagcactgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgctttg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagcctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccggttgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggtaa agtgatcccg ttgcagaaag gcggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatggtt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tagacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgccgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatcttt acgagaagat
    12361 aactaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatccgg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccagact
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctgccgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tgacctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt atcatcctga atttcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagaccg gccagcaggt gcagcagggt
    13201 acttttacca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt catatcgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtctg tttgcaaaaa ctctcgcgca
    13501 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcatcgggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgcgc catctccacc
    13681 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaacagcgta
    13741 ccgagcgcca ccagaaagat tttcgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagcctt
    13861 tctccccagc gcgacagtcc gctaatcagt cccagaatca ggcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ccaggaactg atcgccagat gct
//
LOCUS       DAJTPG010000098        12922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTPG010000098 DAJTPG010000000
VERSION     DAJTPG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..716)
                     /locus_tag="OO230_003131"
     CDS             complement(<1..716)
                     /locus_tag="OO230_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5692793.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIAC"
     gene            957..1757
                     /locus_tag="OO230_003132"
     CDS             957..1757
                     /locus_tag="OO230_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5692794.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1835..2605
                     /locus_tag="OO230_003133"
     CDS             1835..2605
                     /locus_tag="OO230_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5692795.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2653..3999)
                     /gene="mltD"
                     /locus_tag="OO230_003134"
     CDS             complement(2653..3999)
                     /gene="mltD"
                     /locus_tag="OO230_003134"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5692796.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4071..4826)
                     /gene="gloB"
                     /locus_tag="OO230_003135"
     CDS             complement(4071..4826)
                     /gene="gloB"
                     /locus_tag="OO230_003135"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5692797.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4860..5582
                     /locus_tag="OO230_003136"
     CDS             4860..5582
                     /locus_tag="OO230_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5692798.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5579..6046)
                     /gene="rnhA"
                     /locus_tag="OO230_003137"
     CDS             complement(5579..6046)
                     /gene="rnhA"
                     /locus_tag="OO230_003137"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5692799.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6111..6842
                     /gene="dnaQ"
                     /locus_tag="OO230_003138"
     CDS             6111..6842
                     /gene="dnaQ"
                     /locus_tag="OO230_003138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5692800.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            6975..7051
                     /locus_tag="OO230_003139"
     tRNA            6975..7051
                     /locus_tag="OO230_003139"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7009..7011,aa:Asp,seq:gtc)
     gene            complement(7182..8228)
                     /locus_tag="OO230_003140"
     CDS             complement(7182..8228)
                     /locus_tag="OO230_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5692801.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8242..8801)
                     /gene="tssG"
                     /locus_tag="OO230_003141"
                     /pseudo
     CDS             complement(8242..8801)
                     /gene="tssG"
                     /locus_tag="OO230_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8798..10681)
                     /gene="tssF"
                     /locus_tag="OO230_003142"
     CDS             complement(8798..10681)
                     /gene="tssF"
                     /locus_tag="OO230_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5692802.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10697..11191)
                     /gene="tssE"
                     /locus_tag="OO230_003143"
     CDS             complement(10697..11191)
                     /gene="tssE"
                     /locus_tag="OO230_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5692803.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11188..12018)
                     /locus_tag="OO230_003144"
                     /pseudo
     CDS             complement(11188..12018)
                     /locus_tag="OO230_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12005..12919)
                     /gene="tagK"
                     /locus_tag="OO230_003145"
                     /pseudo
     CDS             complement(12005..12919)
                     /gene="tagK"
                     /locus_tag="OO230_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         2965 a         3347 c         3444 g         3166 t
ORIGIN      
        1 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
       61 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      121 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      181 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      241 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      301 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      361 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      421 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      481 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      541 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      601 ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc ccggctaaag
      661 ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc tttcattttt
      721 gctcctgttg caaaatagat gagatatttt gaatttattt gtcattaaac catcaggatg
      781 cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac aggtgtaagc
      841 acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa tccctataac
      901 agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc
      961 gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg atcttaccgc
     1021 cggggtcttt tgcgagcatc ggccaggcat taccacctgg agaaccgtta agtaccgaag
     1081 agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa tggttgtcgg
     1141 tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg cagacaacgc
     1201 cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg cccgctttcg
     1261 tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct
     1321 gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg gtgacggtct
     1381 atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc aacttcagtc
     1441 tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata gctcaccaca
     1501 gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg atgaacgcgt
     1561 tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc
     1621 gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac gtcagcgaag
     1681 cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa ttcagtcccg
     1741 gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg tttggcgctg
     1801 tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc accatgacca
     1861 cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg tacatgcatc
     1921 cggacgagat ttacagcgcc tggcggtgcg tctggctgat tatcctggtg caagtgcact
     1981 tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt
     2041 ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg
     2101 gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat ttgccgataa
     2161 cgcatttgat attgttatca gccgttattc tgcccatcac tggcatgatg ttggtgcagc
     2221 actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga tggacgtaat
     2281 gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat tacgcgatac
     2341 ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg aagccaatct
     2401 gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat gggtcgcgag
     2461 aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga gcgcatcgac
     2521 agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg atatcatcat
     2581 ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg taccttttta
     2641 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc
     2701 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc
     2761 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     2821 gctgttgttt gccagtcgct gtgcaccaat cgtcaaactt tggcctggct tcagcttaga
     2881 tccgcgcagt ttgttccact gctgcaaatc tttggtgctt acgccgagac gtgaagcgat
     2941 gcttgaaagc gtgtcgccag agcgtacggt gtaaacacgg ctgttaagcg gcgtattgtt
     3001 ggcaaccagc gtcgactgta cagcagcaat ttcgcctgaa gcgagagatt cacgcagttg
     3061 atctgcatgc ttctttggca ccatcacgta ctgcggccca cttgcgccca gcgtggagcc
     3121 tttcacgcca gcgttgaatg tcttcagctt gctgacggaa atccccgcca tatctgcaac
     3181 cttcgccatt tcaaccgggc tactcaggtg cacacgcgcc agagcacggc tttcatcggt
     3241 cgttggcaga cgtacgccat aacgcttgct gtttttgaga atatcactca atgccagcat
     3301 tttaggcacg tactgcttcg tttcctgcgg caacggtaac gaccagaagt ccgtggattt
     3361 cccacgcgct ttgttcgttt taattgcctt catgacccga ccttcgccgc tgttataagc
     3421 cgctacggtc agaagccagt cgtcgtcaaa cattttgttc agacgctgca tcatattcag
     3481 cgcggcagtt gttgaagcaa caacatcgcg acgcgcgtca taattgcggg tctgtttcaa
     3541 accataattg cgccccgtgc tcggaatgat ctgccagatg cctgcggcat tggcgccaga
     3601 cgttgcgtga ggatcaaaag cgctctccac tatgggtagt agtaccagtt ccataggcat
     3661 gttacgtttt ttaacttgcc ctgctatcca gtacatatac ggctctgccc gtaaagttac
     3721 atcgtggaga tagctcttat tgcgtaaata tttctgtttc tgttcgcgaa tccggtcatt
     3781 ttccggaatt cccatcttta gctcgtcgcc aatgaaagcc cacaagtcac catctggcgc
     3841 gatagacgtc ccatcgtcca tccatcgtgc ctgacttgta aactttgctg cttccccttg
     3901 accagctgca gaaaggctct gtgcgtgctg ttgaacgttg ccggtactct ggcaacccac
     3961 gagcaggaca gaggcgagta atatcgcttt tgccttcatg tgtgtgtcaa tagttgctta
     4021 aaagacgacc gatcataacg gcgaacggag ccgatgacaa gaaagtttta tcagaaccta
     4081 tctttctttg accttaacca tgcaaaacgc tcttcaggtt gttgcaataa tgtttcttca
     4141 ttaattccat taattaaatc aatatcttcc gttcttaaaa aaacattaat ttgccgctca
     4201 tttttcagaa ttacgggtag tgttatttga ttttttgccc gtaactcctt aactttacga
     4261 taataatcat ttatggacaa atcgtgcgga aaaatactca aagcaaactt catatttgat
     4321 aacgtatatt catgagcaca acataccaat gtatcgtcag gtaacgcact tagcttttta
     4381 agtgattgat acatttgtaa agctgtccct tcaaacaacc gaccacaccc accagaaaac
     4441 agcgtgtcgc cgcaaaatag ataaggttta ctgaagtaac agatatgtcc taaagtgtga
     4501 cccggtgtgg taattacact aaattcatgc cctaaaacga aggcagtttc gccatctttg
     4561 actacctgtg ttgttccctt atcttgtgtc tcttgtggac catacactac aatttgcgga
     4621 aacttttcca ccagttcttt tacgccgcca acgtgatcgt ggtgatggtg ggttagaaat
     4681 atggcctccg gttgccagtt attggcggta atggcgttta atactggctc tgcgtctccg
     4741 ggatcgacaa tcaggcagcg acctgcctca tcattcaaaa cccagatgta attgtcatca
     4801 aaggcgggaa tactgttaag attcatagat tacctctcag tgtaaaacgg aaggttgtga
     4861 tgaaaccggc aagagtccct caaactgtcg tggctcctga ttgctggggc gatttgccct
     4921 ggggagagct ttatcgcaag gcgctggagc gccagctcaa cccgtggttc actaaaatgt
     4981 atggttttca tctgcttaag attggcaatt taagcgcaga aatcaattgc gaagcgtgcg
     5041 cggtttctca tcaagtgaat gtttctgcgc aaggaatgcc cgtccaggtg caggcggatc
     5101 cacttcatct tccttttgcc gataaatccg ttgatatttg tctactggca cataccttgc
     5161 cgtggtgcac cgatccgcat cgtttattac gtgaagccga tcgggtactg attgatgatg
     5221 gctggctggt gattagtggc ttcaacccca tcagtttaat gggattacgc aaacttgtgc
     5281 cggtattgcg caaaacctcg ccctataata gccggatgtt tactctgatg cggcaactgg
     5341 actggctctc tttgttgaat tttgaagtgc tgcacgccag ccgtttccac gttctcccgt
     5401 ggaacaaaca cggaggaaaa ctattgaatg cgcatattcc tgcgcttggt tgcttacaac
     5461 ttattgttgc ccggaaacgg actattcctt taacgctaaa tccgatgaaa cagagtaaaa
     5521 acaagccacg aattcgccag gcggttggag ccacccggca atgtcgtaaa ccacaggctt
     5581 aaacttcgac ttggtagccc gtatcttcca gtgtgggatt catcgccgcg gcacgagcca
     5641 gttcatcaca gcgttcgttt tccgggtgtc cggcatggcc tttaacccat tcccatttga
     5701 tttgatgctg ccccaatgca gcatcaagac gttgccagag atcgacattt tttactggtt
     5761 ttttgtctgc tgttttccag ccacgttttt tccagttatg gatccactgg gtgataccct
     5821 ggcggacata ctggctgtcg gtactcaaaa tgacttcgca atgttctttt aacgcctcca
     5881 gcgcgacaat agcggccatc aactccatac ggttgttggt ggtgcgggtg tagcctgcgc
     5941 taaaggtttt ctcgcgtccg cgatagcgta aaatagcgcc gtaaccccca ggtccaggat
     6001 tacccagaca cgaaccatcg gtgaaaattt ctacctgttt aagcatctct ggtagacttc
     6061 ctgtaattga atcgaactgt aaaacgacaa gtctgacata aatgaccgct atgagcactg
     6121 caattacacg ccagatcgtt ctcgataccg aaaccaccgg tatgaaccag attggtgcac
     6181 actatgaagg ccacaagatc attgagattg gtgctgttga agtagtgaac cgtcgcctga
     6241 cgggcaataa cttccatgtt tatctcaaac ccgatcggct ggtggatccg gaagcctttg
     6301 gcgtacatgg tattgctgat gaatttttgc tcgataagcc cacgtttgcc gaagtagccg
     6361 atgagtttat ggactatatc cgcggcgcgg agttggtgat ccataacgca gcgttcgata
     6421 taggctttat ggactacgag ttttcgttgc ttaagcgcga tattccgaag accaatacct
     6481 tctgtaaggt caccgatagc cttgcggtgg cgaggaaaat gtttcccggt aaacgcaaca
     6541 gcctcgatgc gctatgtgcc cgctacgaaa tagataacag taaacgaacg ctgcacgggg
     6601 cattactcga tgcccagatc cttgcggaag tttatctggc gatgaccggt ggtcaaacgt
     6661 cgatggcttt tgcgatggaa ggagagacac agcagcaaca aggtgaagca acaattcagc
     6721 gcattgtacg tcaggcaagt aagttacgcg ttgtttttgc gacagatgaa gagctggcag
     6781 cacatgaagc ccgtctcgat ctggtgcaga aaaaaggcgg aagctgcctc tggcgggcat
     6841 aaatacatgt aaaaggcgct aaaaatagcg acttgggcga tttttgcagc aaacgattca
     6901 aaagatgaga aaaatcgttg acgaaggtcg aggcaatccg taatattcgc ctcgttccca
     6961 acggaacaca acgcggagcg gtagttcagt cggttagaat acctgcctgt cacgcagggg
     7021 gtcgcgggtt cgagtcccgt ccgttccgcc aatattcaga aagcccgaat cagtaatgat
     7081 tcgggctttc gtcgttttgg tgaagtgaga atttttcact ccgaagtaaa acggatatga
     7141 tttaaaaaat ggccagacat tataaattct ggccacttct gttactccag cgcatcccat
     7201 ttctccagca gttctactgg aatttcattt attacctccg agacgcgttt tccgaccagg
     7261 cgttctgcct ggcgcagcaa aggaatggtc gggctgctcg gttcgctgtg ctcaaaccag
     7321 cgacggatga cacgcaggcg ttccagcgcg tcgtttcggt cgcggatctg ccggggttct
     7381 gcgccggtca tcggtgatat ttcctccgcg atatgcaccg gtagcgcaat ctgctctgcc
     7441 tgcatctgtt cagtatgaac gatggtggcg ttttccggct gctcagcctg tggttgtggc
     7501 aggatctccg gcgtggtgct ctgcactgct cccggtaaca gggccagcag ttgcgtcaga
     7561 cgggagaagt ccggtgcctg gtcattcagc gtttcacgcg catggcgttg cagccgctca
     7621 gtggtctctg ctgcctggcg aaaggcatcc agcggcagtg tgccgcgcgc ttccaggtct
     7681 gccagttgct ggcgtaccga ttctggtgcc agcgcatccg ccgggcgtaa tgcagatagc
     7741 gaacgctcca catcccgcac ctgaaggcgc atggcagcat tatttgacag tgtaatgcca
     7801 cgaatatccg ccattacacc ttcgtggtcc agcaacgccg ccagcgcgtt actgcgtgcc
     7861 accgcagcat cttcggcggt gatatcttca gtcgcgagca gttgaggatg gatggcgtcc
     7921 gggtacgtgg agcacaacgt ttccagcagc gtgagcgcct ccgacagtcc cctggcaccg
     7981 gcctgctgga tacggcagcg cagcaggaca accagaatgc ggatatcttt gctgcgcatc
     8041 agcagacgat gggcgtcccg ctcaagctcc gcccagttaa tgttttccgg cgtactgaca
     8101 aaatcgccat attgcgcttc ttcacgcggt gcggcgcggg tgaacagcag caggtattcc
     8161 ggatcgtact ccagatccgg gccgcagggg cattccgccg aaattgggtc agacaggaat
     8221 atttccattt aattatcctc agtgcgccag cgtggtggcc agaccgtccg gattgctgga
     8281 ctcacgcacc agatgggccg atgccgccag acgagcgtga tctggcaacg ggctgtcagc
     8341 gctctcccgc aaatcctgcc cgctgaggct ggcgacataa aaggcaaagc ggtcatgttg
     8401 cggtttatcc agaccgcccc cagcggactg ggcgctgcgc catgcctggt aaaactgcgt
     8461 cagccagcgg tgatgaaaaa gatcagaaaa acgggtcagc gtcggctttc agtccggtta
     8521 cgcgccagtt cggtgtagtg cagcggcagt ggaccgttcg gcccccacag cacgaggctg
     8581 tacagactga tgtgcaggcg cccttcctgc cagcccacac tggcgatttc tcgtggcgca
     8641 aaggtcagcg cgggcgtttg tccgaggcga aatttttcca tccgtggttg ccaggtgttg
     8701 cccgccggaa cggtacacaa ctgcacatcc acgcggcgca tcaggttcag aaaaccgtaa
     8761 cgccaggggg gggcctgcgc ctgagtgaaa tcgttgctca tacgcttccc cgctgtcccg
     8821 ccctgacagg ccaggtcata attttgccgc gctgcatgct gtgcagcgtc atctgcgaga
     8881 aggtattaat ggaaacatgg cgggcgatat agtgttccag caccaggccg aacagatacg
     8941 gactgatgcc ggaaaaacct tcttcatcca ccgtcagttc acaactgacg ccacgcccat
     9001 agaccagtag tccggaaccg ggcagacggc gggtgaccgg ggtcgtttta caaccaatca
     9061 ggctgcgcac ctggcgcgat tgcggactgt catgagcagg aataaacagg ttcagcagat
     9121 ctcgcaacgc ctggccgccg gtacggtgat ccagatcggc cagcggcaga taattaaaag
     9181 ataactggcg gatcaaccgc catgccatct cacgttcagc catcgggggc tgcggcgagc
     9241 gtggcgggcg gataagccct acgcccgcca ccgggatggc tgcatccacg gttagatcgt
     9301 cccggccatt gcgggcaata agacagggca gatcgcggtt agtgaccatg gccgtgatgg
     9361 tgatgtggcg cagattttcc gggtacggcg cttcatactg gtcaaccagc gacaggaaca
     9421 cttccgagcc cgtatagggg gtacgggtgc cgtaacggcg ggcgttttct gataagcggc
     9481 ggggctcccg gcgcagggaa aaataacgtc cgtgattgcc ttcatcatta ttgcgggtgt
     9541 ggtagagcgg gcgaaaggcc atcttccgtg tggtgtcggt ttccagccct tccacttcct
     9601 ggaccgagaa cacctcataa tccagcggat gggttctgtc caccaccaga tgctgttccg
     9661 tcgtgctgtg cgtgacatcg atccgggcgg tgacgcgggg aaacaggttg ataaccgggg
     9721 tacagaacag gctgaactgt gccgcatttg tctggtggat cagccagtcc ggcggcaggc
     9781 ggttcagcag gataacaatc tccgccacgc caccgtcaat tttctgtaat ccggcagaca
     9841 ggcctgtcgg ggtgaaaaaa tagaagcgtt ccggacaggc aaaatattca tgcagcaggt
     9901 tatgaccgtg aaacacgttc cacgccagcg gcagcagccc ctgatccggc tccaggcctt
     9961 cgtacataac gggctgttgc agattcacat ccagcgcgcc gtcaaaatgc cccggaatgc
    10021 cagccagggg cgcgatggcg ctggtatgta gaagttctaa caggtgtgag gccgtacgct
    10081 cctcgccgca gagataaaac ggcagcctgt ccagtccggc aagctgactg aacgttagtt
    10141 caccaaaggt gcgcagggta atacgcagcg ccccggcgac gtgaatgttg gccggcaggt
    10201 agcggtgcag ggcaggcata tccggtggcg cggcggtcag tcgcgcctct tcgatggcga
    10261 gcggccacag cgttacatcc tggctgctgc gaaactggca ggcagtgctt tcgccttccg
    10321 gaatggcgga gaaaaaggct gtgtcgcgcg ggacggtaaa tcctttcgcc agatcgcctt
    10381 cttccgtatc gggatgcaac tgcgccacgc tcattgaggg cgtgggtgtg acgtaattgg
    10441 ggctgaccac ttccagcagg cgctgggtga aacgggggaa ttcagcgtca atttttagct
    10501 gcgtgcgggc ggtcaggaag ctgaaggcct cgaccattcg ctctacatac ggatcggcaa
    10561 tatccgttcc ctgcatgccc agccgggcgg cgactttggg atgacgggcg gcaaattcgg
    10621 ctcccgtttc ccgcagatag ctcagttcgc ggttgtagta ttccagtagc cgtggatcca
    10681 tcggtctctc tctgaattaa aaaaacgtaa tgcgactctg ctccatatcc agcgcgctac
    10741 gtacccggaa tgcagtcgga tacggctgcg tgaggatctc gccgcgaatt tcaaactgta
    10801 atgtgttatg gccactctgg cggcgctcat cctgaagtgg gatgatttgc agagtggacg
    10861 ctttcagccg gggttcaaac cgggtgatgg ttcgcaaaat ggccctgcgg atatcgtccc
    10921 acttgtgctc atacagaaag ctgcccgcca gtggcggcag gccgtagtta agtacggacg
    10981 ctgccgcgta cggataacgt gcagcatcaa tatcatcttc atggctgacg gtgttgagca
    11041 gaaatgacaa atcccggcgg atgatctcct taatctgaac cggactgacg ctgataccgt
    11101 ggtcgcgttt ctggtttggg gcgtcgtcac acaggcggtc aaacagcgtg ggcagcaggt
    11161 gattgcctgg acgctggcgg gaagcgctca cgcgtcgctt ccttcagcgg tgttaaaggt
    11221 acaggtggac aaatccagca ggctgatatc gccgtgaccg gtgagccaca ccctctgacc
    11281 cagcgcctgt accagtgtct ctcccggacc gtcctgccag accgtttccc ggcacagacg
    11341 cagggagtcg gcagcctttt cagagccgct gtagcgggga aacagctagg cactgtgtgt
    11401 atcgccattg ttcagggtga cgttgacggg tttccagatc aaatcgatca gatgcgcagg
    11461 ccgcggcgat ttcagcgaac agatttgtga aaacggcagc cagatatatg ctccgcccgc
    11521 gatcagttcg agtaccggac cggtacggga gtcgctgtcg ctaatccagt caaatgcgcc
    11581 gccattccac tgcccggaag tgtctgttat ggcctcaagc gcgcggctgc ggtgagcatc
    11641 agcttcctgc gcttcaccac gggcgttaca ggcgagggcg gtcagcagat cctccatcca
    11701 ggcgggcggc gggagaataa caccgggccg ctgttcgccc tgaaagcagg cgtggcggta
    11761 tatctcacag cggatcagct cgccaaatac ctgcgcttcg cgagtataat ttgcgtccat
    11821 ccgggcgcat agctggatct gttgcagtgc ccgcgaccag tcgcctgcca cgcacaataa
    11881 ctgaaacagg ctgtggcggc gggaggcctg cgctgggtgc atcctgacct gttgctctgc
    11941 catctgaatg ccctccacca cggaataatc ccgcagcagc gccgacaagg taatcggtag
    12001 ctcgttattg tttgtcatga tatatctctc catgttgtgc cgggtttatc cggtaatggc
    12061 tgtcaatgcc aatgatgcga tgctctttcc gtgtcagatc tggcagtacg gtgttatgcg
    12121 ctgtttccgg ggcgtattcc ggggaaagta aatgcaggat atcaggggga gtcttttcga
    12181 caggccagat agtttcatct tcgccagtgg catccagtac attgagaata gcgtcaatgc
    12241 ttaacccacc agtgaccata tcctgcaatg tctcggcttc ggtgaccagg tcacgcaatg
    12301 aatccagatc ctgcgaggag ggctcttcgt taaaaggaaa aggatcagca tggcgattaa
    12361 gatcttgttc gggagataac aacgcctcgc ggtattcctg ataaagtcgg gtcagggcat
    12421 ttcctttttc cgcaccttct ccggtggagg aggctccatg aacaggaatg atatcgaatg
    12481 gattttgtga tgtcagatgc tgctgcctga accaatccag atccagatat tcggttattg
    12541 cttccgacgt gatggcagtc tgcgatatcg tttttactgt gctcaggttc ggagatgatg
    12601 gattgccatg tacaaggcac tatgaagata acccccattc tatcaggtcg ccttcattca
    12661 gacgcatacg gtgatgaggt tccacaatct ggtcgtttac cgtacagcaa tattcggtag
    12721 actgattgat aatccaccat gcttcttcgt ggcgggtcag agtgagggca atctttacat
    12781 catcattcac cttaaaaaca ggaatccagg gagaggtaac ggtgaagata atttgtgttc
    12841 cttcagtcgc gtaaattgtg ttatcggcga tcccgtgcgc ctgtattttt tggagtcccc
    12901 acgtaatatt tggtttcatt gt
//
LOCUS       DAJTPG010000099        12917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTPG010000099 DAJTPG010000000
VERSION     DAJTPG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..605
                     /gene="mxiE"
                     /locus_tag="OO230_003146"
     CDS             <1..605
                     /gene="mxiE"
                     /locus_tag="OO230_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5692804.1"
                     /translation="NIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALVEHSHAEKCIH
                     FYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLVLYLLRTIEKE
                     KEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVFLHNQTITSAA
                     MNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            592..2292
                     /gene="mxiD"
                     /locus_tag="OO230_003147"
     CDS             592..2292
                     /gene="mxiD"
                     /locus_tag="OO230_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5692805.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            2311..3378
                     /gene="mxiC"
                     /locus_tag="OO230_003148"
     CDS             2311..3378
                     /gene="mxiC"
                     /locus_tag="OO230_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5692806.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            3391..5451
                     /gene="mxiA"
                     /locus_tag="OO230_003149"
                     /gene_synonym="virH"
     CDS             3391..5451
                     /gene="mxiA"
                     /locus_tag="OO230_003149"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5692807.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            5464..5865
                     /gene="spa15"
                     /locus_tag="OO230_003150"
                     /gene_synonym="spaK"
     CDS             5464..5865
                     /gene="spa15"
                     /locus_tag="OO230_003150"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5692808.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            5869..7161
                     /gene="spaL"
                     /locus_tag="OO230_003151"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             5869..7161
                     /gene="spaL"
                     /locus_tag="OO230_003151"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5692809.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            7254..7592
                     /gene="spa13"
                     /locus_tag="OO230_003152"
                     /gene_synonym="spaM"
     CDS             7254..7592
                     /gene="spa13"
                     /locus_tag="OO230_003152"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5692810.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            7579..8457
                     /gene="spa32"
                     /locus_tag="OO230_003153"
     CDS             7579..8457
                     /gene="spa32"
                     /locus_tag="OO230_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5692811.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            8451..9332
                     /gene="spa33"
                     /locus_tag="OO230_003154"
                     /gene_synonym="spaO"
     CDS             8451..9332
                     /gene="spa33"
                     /locus_tag="OO230_003154"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5692812.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            9337..9987
                     /gene="spa24"
                     /locus_tag="OO230_003155"
                     /gene_synonym="spaP"
     CDS             9337..9987
                     /gene="spa24"
                     /locus_tag="OO230_003155"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5692813.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            10002..10262
                     /gene="spaQ"
                     /locus_tag="OO230_003156"
     CDS             10002..10262
                     /gene="spaQ"
                     /locus_tag="OO230_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5692814.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            10262..11032
                     /gene="spa29"
                     /locus_tag="OO230_003157"
                     /gene_synonym="spaR"
     CDS             10262..11032
                     /gene="spa29"
                     /locus_tag="OO230_003157"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5692815.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            11039..12067
                     /gene="spa40"
                     /locus_tag="OO230_003158"
                     /gene_synonym="spaS"
     CDS             11039..12067
                     /gene="spa40"
                     /locus_tag="OO230_003158"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5692816.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            12080..12322
                     /locus_tag="OO230_003159"
     CDS             12080..12322
                     /locus_tag="OO230_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692817.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         4150 a         1830 c         2516 g         4421 t
ORIGIN      
        1 aaaatataaa attcagtgat aatgtgaact atcattatcg ctttaacatt aatagttgtg
       61 caaaatttct tgctttttgg gactatttca gtggtgctct agtagagcat agccatgctg
      121 aaaaatgtat tcatttctat catgagaatg atcttcggga tagttgtaat accgaatcga
      181 tgcttgataa gttaatgtta agattcatct tttcatctga ccagaatgta agtaatgcac
      241 tggctatgat acgaatgact gagtcatatc atttggttct ttatcttctg agaactattg
      301 agaaagaaaa ggaggtaaga ataaagagtc tgacagagca ttatggtgtg tcggaggctt
      361 attttagatc tttatgtaga aaagctttag gggcaaaagt caaagaacaa ctgaacactt
      421 ggcggttagt aaatggttta ttagatgtat ttttgcacaa tcaaacaatc acttcagctg
      481 ccatgaataa tgggtatgca tcaacatccc atttttcaaa tgagataaaa acacgactag
      541 gcttttctgc aagagaatta tcgaatatta cttttttagt gaaaaaaata aatgaaaaaa
      601 tttaatatta aatctttgac tctcttgatt gtattgttac cattgatagt taatgctaat
      661 aatattgact ctcatttatt agaacaaaat gacatagcga aatatgttgc tcaaagtgat
      721 acggtaggat ctttttttga acgtttttct gcgttattaa attatcctat tgttgttagc
      781 aaacaagctg caaaaaagag aatatctggg gagtttgatt tatctaatcc agaagaaatg
      841 cttgaaaaac tgacattact tgtgggactc atatggtata aagatggaaa tgcattatac
      901 atatatgact ctggagaatt aataagcaaa gttattttgc tggagaatat atcacttaat
      961 tatcttattc agtatctgaa ggatgctaac ctttatgatc acagatatcc tataagagga
     1021 aacattagtg ataaaacatt ctatatatct ggtcctccgg cattggttga actggtagct
     1081 aatacagcaa ctttgcttga taagcaggtt tcttctattg ggacagataa agttaatttt
     1141 ggggttataa aattaaaaaa tacatttgtt agtgacagaa cttataatat gagaggggag
     1201 gatattgtca tcccaggggt ggctactgtt gtagaacgtt tattaaataa tggtaaggct
     1261 ctgagtaaca gacaagccca aaatgatccg atgccaccct ttaatattac acagaaagta
     1321 tctgaggata gtaatgactt ttcatttagt tcagttacca atagttctat attagaggat
     1381 gtatcactta ttgcatatcc agaaaccaat tctatattag tcaaaggtaa tgatcagcaa
     1441 atacagatta ttcgcgatat tatcactcag ttagatatag ccaaacgaca tatagaatta
     1501 tcattatgga taatcgatat tgataaatct gaattaaata accttggtgt taattggcaa
     1561 ggaacggctt catttggtga ttcgtttggt gcgtcattta atatgagctc atcagcatcg
     1621 ataagcactt tggacggcaa taagtttatt gcttcagtta tggcattaaa tcagaaaaag
     1681 aaagcaaatg tagtttcaag gccggtgatt ttgacacaag agaatattcc ggctatattt
     1741 gataataaca ggacttttta tgtgtcatta gttggtgagc gcaattcttc gttggagcat
     1801 gttacgtatg gaacattgat aaatgttata cctcgctttt catctcgggg acaaattgag
     1861 atgtctttaa cgatagagga tggaacagga aatagtcagt caaattacaa ttataataat
     1921 gaaaatacat ctgtccttcc agaagttgga aggaccaaaa tcagtacaat agcgagagtt
     1981 ccacaaggca aaagtttgtt gataggtggt tatacgcatg aaacgaactc taacgaaata
     2041 atatctattc cttttctctc ctctattcct gtaatcggta atgtgttcaa gtataaaaca
     2101 agtaatatca gtaatattgt aagggttttt ctgattcaac cccgtgagat aaaggagtca
     2161 agttattata atactgcaga atataaaagc ctcatatctg aaagagaaat acaaaagact
     2221 acacaaataa tcccatctga aactactttg ctggaagacg aaaaatcatt ggtttcatac
     2281 ttaaattact aactataaag taggtgatgt atgcttgatg ttaaaaatac aggagttttt
     2341 agctctgcat tcattgataa gttgaatgca atgacaaatt cagatgatgg agatgagact
     2401 gctgatgcag agcttgattc tggcttggct aatagcaagt atattgactc atctgatgag
     2461 atggcttccg ctctttcgtc atttataaac agaagagacc ttgagaaact gaaaggaaca
     2521 aatagtgata gtcaggaacg tattttagat ggggaagaag atgaaattaa tcacaagatt
     2581 tttgatttaa agagaacgtt aaaagataac cttcctctag atcgggattt tatagacaga
     2641 ctaaagagat attttaaaga tccaagtgat caagtcttag cattaaggga acttttgaat
     2701 gaaaaagatc ttactgctga acaagtcgaa ttattaacta aaattattaa tgagataata
     2761 tcaggtagtg aaaaaagtgt taatgctgga ataaattcag ctatacaggc taaattattt
     2821 ggcaacaaaa tgaaacttga accacagctt ttgcgtgcat gttatcgtgg ttttatcatg
     2881 gggaacatat caacaacaga tcagtatata gaatggcttg gtaattttgg ttttaatcac
     2941 agacatacaa ttgtgaattt tgtagagcag tcactgattg tagacatgga ttctgagaaa
     3001 ccgagctgta atgcttatga gtttggtttt gtgttatcta aattaattgc aattaagatg
     3061 attagaactt cagacgtaat ttttatgaag aaactggaat cctcaagctt gctaaaagat
     3121 ggcagtttaa gtgcagagca gctattgcta actttattat atatttttca atatccaagt
     3181 gaaagtgagc aaattcttac ttctgttata gaagtatcac gagccagtca tgaggattct
     3241 gtagtgtatc aaacatatct atcttctgtt aatgaaagtc ctcatgatat atttaaaagt
     3301 gaaagtgaaa gagaaattgc gatcaatatt ctacgagagc ttgtcacaag tgcatacaag
     3361 aaagagcttt ctagataaca ggagataaaa gtgatccagt cttttcttaa gcaagtaagt
     3421 actaagcctg aattaattat attagtgctt atggttatga tcattgctat gttgatcata
     3481 ccattaccta cataccttgt tgatttttta atagggctta atattgtact tgctattctc
     3541 gtttttatgg gatcatttta tattgaaaga atattgagtt tttctacatt cccttcggtt
     3601 ttgttgataa cgacattatt tcgtctcgct ttatcaataa gtactagtcg actaatttta
     3661 gttgatgcgg atgcgggtaa aattattact acatttggcc agtttgtcat tggtgatagt
     3721 ctggctgtag gttttgttat tttttcaata gtaactgttg tgcagttcat tgttataaca
     3781 aaaggatctg aaagggttgc ggaggtagct gctcgctttt ctcttgacgg tatgccgggg
     3841 aaacaaatga gcattgatgc ggatttaaag gctggaatta ttgatgccgc aggagctaaa
     3901 gaaagacgca gtattctgga acgtgagagt caattgtatg gttcatttga tggagcaatg
     3961 aagttcatta aaggtgatgc aattgctggc atcattatta tttttgtcaa tttaatagga
     4021 ggtatttctg ttggtatgag tcaacatgga atgtccctat ctggtgcatt atctacttat
     4081 accatactaa ctattggtga cggattggtc tctcagattc ctgctctgtt aatttctata
     4141 agtgcaggat ttatcgttac aagagttaat ggtgatagcg ataatatggg acgtaacata
     4201 atgtcccaga tatttgggaa tccttttgtt ctcattgtta catcagctct tgccttggcg
     4261 atagggatgt tgccaggttt tccgtttttt gtttttttcc tgatagcagt tactttgacg
     4321 gctttattct attataaaaa ggtcgtagaa aaagaaaaaa gtctgtctga gtctgattct
     4381 agtggctata ctggtacatt tgatattgat aatagccatg actcatcttt ggcaatgata
     4441 gaaaatctgg atgctattag ttcagaaacc gttcctttaa ttttattatt tgccgaaaat
     4501 aagataaatg caaatgatat ggaaggtctt attgaaagga taagaagtca gttctttatt
     4561 gattatggtg tcaggcttcc aactatttta tatagaacaa gcaatgagct taaggtcgat
     4621 gatattgttt tgttaataaa tgaggtgcgt gccgatagtt ttaatatata ttttgacaaa
     4681 gtgtgtatta cagatgaaaa tggagatata gatgctctag gtattcctgt tgtttcaact
     4741 tcatataatg aacgtgttat ttcttgggta gacgtttcat atacagaaaa tctaactaat
     4801 attgatgcta aaattaaaag tgctcaagat gaattttatc accagttgtc acaagcttta
     4861 ttaaacaaca taaatgagat ttttggtata caagaaacaa aaaatatgtt agatcagttt
     4921 gaaaatcggt atcctgatct attaaaggaa gtcttccgac atgtgactat acagagaatt
     4981 tctgaggtat tacaaagatt gcttggagaa aatatttctg ttcgcaattt aaaacttatt
     5041 atggagtctt tggcgctttg ggctcccaga gaaaaagatg tcataacatt agttgaacat
     5101 gtccgtgcat cactttctag gtatatttgt agtaaaatag ctgtttctgg tgagattaaa
     5161 gttgtgatgc tttccggata tattgaggat gcaataagaa aagggataag gcaaacctct
     5221 ggtggctctt tcttgaatat ggatatagag gtttcggatg aggtaatgga aactttagca
     5281 catgctttga gagaattgag aaatgcaaaa aaaaatttcg ttcttttggt atcagtagat
     5341 atacgtaggt ttgttaaaag acttatagat aacagattta agagtatact cgttatatcg
     5401 tatgctgaga ttgatgaagc atataccatt aatgtattaa agactattta gtgaggttta
     5461 aatatgagta acattaattt agttcaatta gttagagata gtcttttcac gattggttgt
     5521 cctccatcaa ttattacaga tttagacagc cattcagcga ttaccatttc tttggatagt
     5581 atgcctgcta ttaatatagc tcttgtcaat gagcaggtta tgttatgggc taattttgat
     5641 gcaccaagtg atgtaaaact tcagtcatct gcttataaca tattaaattt aatgttaatg
     5701 aactttagtt atagcattaa tgagctggta gagcttcatc gttctgatga gtacttacag
     5761 ttaagagttg ttattaaaga tgactacgtt cacgatggga ttgtttttgc agagattctg
     5821 catgagtttt atcagaggat ggaaatctta aatggggtct tataatcaat gagctataca
     5881 aaattgctca ctcaattatc ttttcctaat agaatctcgg ggccaatctt ggaaacaagt
     5941 cttagcgatg tttcgattgg tgagatttgt aacattcagg ctggaattga aagtaatgaa
     6001 attgttgcaa gagctcaggt tgtaggattt catgatgaaa aaacaatatt aagcttgatt
     6061 ggaaattctc gtggactttc acggcaaacg ttgattaagc ccactgccca gtttcttcat
     6121 acgcaagtcg gccgtggatt attgggagca gtagtcaatc ctttagggga ggttactgat
     6181 aagtttgctg ttacagataa cagtgaaatt ctttatcgac ctgtagataa tgctcctccg
     6241 ctatatagtg aaagggctgc aattgagaag ccttttttaa caggtattaa ggttattgat
     6301 tctttactca cgtgtggtga aggacagcga atggggattt ttgcgtcagc tggttgtggc
     6361 aaaacttttc tcatgaatat gctcattgaa catagtggtg ctgatatata tgttattggg
     6421 ttaattggtg agcgaggtcg agaggttact gaaacggttg attatttgaa aaactctgag
     6481 aaaaaaagca ggtgtgtttt agtatatgca acttcggatt actcttcggt tgatcgttgt
     6541 aatgctgcat atatagccac tgctatagcc gaatttttta ggactgaagg acataaagta
     6601 gcgcttttta ttgattcatt aacaaggtat gccagagcat tacgtgatgt ggccttagcc
     6661 gctggagaat cacctgccag aagaggctat ccggtttcgg tttttgatag cttacccaga
     6721 cttcttgaaa ggccaggaaa gttaaaggca ggtggctcta ttactgcatt ttacactgtt
     6781 cttttggagg atgatgattt tgctgatcca ttagctgaag aggtaagatc cattttagat
     6841 ggacatatat atttgagcag aaatctagcc caaaaaggac aatttcctgc aattgattcc
     6901 ttaaaaagta taagcagggt atttacacag gttgttgatg aaaaacatcg tattatggcc
     6961 gctgcatttc gggagctact ttctgaaata gaagagctaa ggacaattat tgactttggt
     7021 gaatacaaac cgggggagaa tgcctctcag gataaaatct acaacaaaat atctgttgtt
     7081 gagagttttc tgaagcaaga ttatcgactc ggttttactt atgagcagac aatggagctt
     7141 attggtgaaa caattagata aggttttaaa aatcaaagat aaatatcaac ggtctgttaa
     7201 attaattgaa gcacacatat taacactaaa aaaaaaactc tttgtatcga gatgtggagg
     7261 cattagataa aaggattata tacttcctgc aactggagaa tgacttagaa cctgtaggcg
     7321 ctcagagtgt tagtcagctt ttcaatacaa gacgtaagat cgctatagta aaaaagcata
     7381 ttatccaata tcagtctgaa cgaattcttc taaagggaag aattgaagaa attcaaaaag
     7441 atattgatga ggcaaatgct tctaaaagaa aactgcttca taaagagagt aaaatatgta
     7501 aacggattgg cttgattaaa agaaataact ttgccaaaca gttgatactt gatgagttat
     7561 cacaagagga tatgaagtat ggcattagat aatataaacc taaattttag tagtgacaaa
     7621 cagatagaaa aatgtgagaa actatcttca atagataata ttgatagtct cgttttgaag
     7681 aagaagagga aggttgaaat tcctgagtac tctttaattg catctaacta ttttactatt
     7741 gataagcact ttgaacataa gcatgataaa ggagaaattt atagtggcat taaaaatgcg
     7801 ttcgaactta gaaacgaacg agcgacatat tctgatattc cagaatcaat ggccattaaa
     7861 gaaaatattt tgataccaga tcaagatatc aaagcaaggg aaaaaataaa tatcggcgat
     7921 atgaggggga tcttttcata taataagagt ggaaatgcag acaagaactt cgaaagaagt
     7981 catacttctt ctgtaaaccc tgataatctg ctagaatctg ataatagaaa tggtcaaatt
     8041 ggtttaaaaa atcatagctt gtctattgat aagaatattg ctgacatcat ttctttacta
     8101 aatggaagtg ttgctaaatc atttgagctg cctgtaatga ataaaaatac tgcagacata
     8161 accccatcca tgtcattgca agaaaaatca atagttgaaa atgataaaaa tgtttttcaa
     8221 aaaaatagtg aaatgactta ccactttaaa cagtgggggg ctggacattc tgttagtatt
     8281 tcagtggagt ctggttcttt tgttctaaaa ccgtcagatc aatttgtagg aaataaactt
     8341 gacttaattt tgaaacaaga tgctgagggt aattacagat ttgatagcag tcaacataat
     8401 aaggggaata aaaataatag tacaggatat aatgaacaga gtgaagaaga atgctaagaa
     8461 ttaaacattt tgacgctaac gaaaaactac agattttata tgcaaagcaa ctctgcgagc
     8521 gtttttcgat tcagacattc aaaaataaat ttacaggcag tgaaagttta gtcactctta
     8581 cttccgtgtg tggggattgg gtaattcgta ttgatacatt atcttttttg aaaaaaaaat
     8641 acgaggtatt ttcaggattt tctacacaag aatctttact gcatttatca aaatgtgtct
     8701 ttatagagtc gtcatctgta ttttcgattc cagaactgtc tgataagatt actttccgga
     8761 tcacgaatga aatccagtat gcaactactg ggagtcattt atgctgtttt tcctcttctt
     8821 taggtattat ttattttgac aagatgccgg tattacgtaa tcaagtttct cttgactcat
     8881 tgcatcatct tttagagttt tgcttaggtt catctaatgt aaggctggct actttaaaaa
     8941 gaattcgcac tggtgatata atcatagttc agaaacttta taatttatta ttgtgtaatc
     9001 aagttattat tggggattat attgtgaatg ataataatga ggcaaaaatt aatctgtcag
     9061 aaagtaatgg tgagtcagaa cacacagaag tttctttggc attattcaat tatgatgata
     9121 tcaatgtaaa agtggacttt attcttttag aaaaaaatat gacaatcaat gaactaaaaa
     9181 tgtatgtaga aaacgaatta ttcaagtttc ccgatgacat agttaaacat gtaaatatta
     9241 aagtaaatgg ttctttggtt gggcatgggg aacttgtttc tattgaggat ggttatggta
     9301 tcgagattag ttcttggatg gtaaaggagt aatctcatgc tgagtgacat gtccctcatc
     9361 gctacacttt ctttttttac gctattacca tttttagttg ctgctggtac ctgttatata
     9421 aagttttcta ttgtttttgt aatggtacga aatgcacttg gtttgcagca ggttccatca
     9481 aatatgactc tcaatggtat tgcattgatt atggctttgt tcgtgatgaa acccattatt
     9541 gaagctggtt atgaaaatta tctaaatggc cctcaaaaat ttgatacaat atctgatatt
     9601 gtccgttttt ctgattctgg cttgatggaa tataagcaat acttgaaaaa gcatacagat
     9661 cttgagttag cacgtttttt ccaaaggtct gaagaagaaa atgctgattt aaagagtgca
     9721 gagaataatg attattcatt gttttccctc ttgcctgcat atgcgctaag tgaaatcaaa
     9781 gatgcgttca aaataggttt ttatttatat ttgccatttg ttgttgttga tcttgttatt
     9841 tcaagcattt tacttgcact tggtatgatg atgatgagtc caatcacaat atcagtacca
     9901 ataaaacttg tattatttgt tgcgcttgat gggtggggta tcctgtctaa ggccttgatt
     9961 gaacaatata tcaatattcc tgcttagatg atggaggtaa tgtgtctgat atagtttata
    10021 tgggtaataa ggctctttat ttaatcctta tcttttcctt atggccagta ggtatagcta
    10081 cggttattgg attatctatt ggtttgttac agacagtgac tcaactccaa gagcagacac
    10141 ttccttttgg tataaagctt ataggtgtct caatatcttt gctacttctt tctggatggt
    10201 atggtgaggt tttattgtct ttttgtcatg aaataatgtt tttaattaag agtggggttt
    10261 gatggacatt tcaagctggt tcgaaagtat tcatgtgttt ttaatactcc tgaacggcgt
    10321 tttttttaga ttggctccat tatttttctt tcttccattt ttaaataacg gtataatttc
    10381 tccatctatt agaatacctg tgatttttct tgttgcatca ggattaatta cttctggtaa
    10441 ggtagacata ggttcttctg tttttgaaca tgtttatttc cttatgttca aggaaataat
    10501 tgttggcctc cttctctctt tttgcttgtc tcttcccttt tggatatttc atgctgttgg
    10561 tagcattatt gacaaccagc gtggggcaac gcttagtagt tcaattgatc ctgccaatgg
    10621 tgttgatacg tctgagttgg caaaattttt caatcttttt tctgcagttg tatttctata
    10681 cagtggtggt atggtcttta ttttagaatc catacaattg tcttataata tatgcccgtt
    10741 attttctcaa tgttctttcc gtgtctcaaa tatcttaaca tttctgactt tattggcaag
    10801 tcaggctgtt attttagcca gtcctgttat gatagtattg ttactatcag aagtattact
    10861 tggtgtatta tcgagatttg ctccgcagat gaatgctttt tccgtatcat taactattaa
    10921 aagtttactt gcaatattta ttattttcat ctgttcttct actatttact tttctaaagt
    10981 tcaatttttc ctcggtgaac ataaattttt cacaaatcta tttgttagat aaaatattat
    11041 ggcaaataaa acagaaaagc cgacacctaa aaaactaaag gatgccgcaa aaaaaggaca
    11101 gtcatttaaa tttaaggatt taacgactgt tgttattatt ctggtaggga catttactat
    11161 aatatcattc ttttccttaa gtgatgtaat gctcttatac agatatgtaa taattaatga
    11221 cttcgaaatt aatgagggta aatacttttt tgcagtggtt attgtctttt ttaagataat
    11281 tggcttccca ctttttttct gtgttctttc ggctgtgttg ccaacattgg ttcaaacaaa
    11341 gtttgttctt gcgactaaag ctatcaagat tgatttttca gtattaaacc ctgttaaagg
    11401 gttaaaaaaa atatttagta taaagacaat aaaagaattt ttcaaaagca ttctgcttct
    11461 tattattcta gcattaacaa cctatttctt ttggattaat gaccgaaaaa taattttttc
    11521 tcaggtgttt tctagtgttg atggcttata tcttatttgg gggaggctgt ttaaggatat
    11581 aatattattt ttcttggcat tttctattct tgttattatt cttgactttg tgattgagtt
    11641 cattttatac atgaaagata tgatgatgga taaacaggag ataaaaagag aatatataga
    11701 gcaagaggga cactttgaga caaagtcgag aaggcgtgag ttgcatatcg agattctttc
    11761 agagcagact aaatctgata tacgtaattc aaaattagtg gtaatgaacc cgactcatat
    11821 tgcaattggt atttatttta atccagaaat agcgcctgca ccttttattt ctctcattga
    11881 aactaaccag tgtgccttgg ctgtcagaaa atatgcaaat gaagttggta taccgactgt
    11941 gcgtgatgtg aaattagcta gaaaactata taaaacacat acaaaatata gttttgttga
    12001 ttttgaacac ttggatgaag tcctacgtct tattgtttgg cttgagcagg ttgaaaacac
    12061 tcattaataa gtaaaggaga tgtgttatat gggagttaat ttctgtaata aaataggtat
    12121 tgatcagtct gaatttgaaa tagaatcttc catcattaac tccattgcta atgaggtatt
    12181 gaacccaata tctttcctta gcaataagga tataataaat gttttactca gaaagatttc
    12241 atctgaatgt gacctggtta gaaaagacat ttatcgttgt gctctggagt tggtcgttga
    12301 aaaaacacct gatgatttat aatcattcat aataactctt ttgctgatat aaatattttt
    12361 gttgaagata tttatgtagt cacaggatca attgtagaaa attgttatgt ttttgttagg
    12421 aactgcacta aataatatat gataagacag caaaaacgac tgaccataat ccttctttta
    12481 ttaggagttg ataagagaga ctattcatcc tgtaatgtta aaactctgtt atatagtatt
    12541 cgcgattatg caaagtctgt aaatgatcat gagatactaa ccgagagtaa tcgtttatta
    12601 tctcattgca tttcagatag taacggagct ttctttaaaa gtagtaaata cgttcccctt
    12661 aagtacttac gtaaaaggag aatagcacga aagataccaa atgattagca ctttgctagt
    12721 aagcttgctg gtaaactata ttgaccgttg ggtttgtttt gagactgtaa tttaaattgt
    12781 gtaattgcct gtttttgata tgtttactcc aacaacggag acaggtaaat tatggacgaa
    12841 aagaagctta aagcccttgc ggctgaactg gctaaaggcc tcaaaactga ggccgacctc
    12901 aatcagtttt cccgtat
//
LOCUS       DAJTPG010000100        12906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTPG010000100 DAJTPG010000000
VERSION     DAJTPG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO230_003160"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OO230_003160"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5692818.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="OO230_003161"
     CDS             complement(1806..3224)
                     /locus_tag="OO230_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5692819.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="OO230_003162"
     CDS             complement(3378..3722)
                     /locus_tag="OO230_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5692820.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO230_003163"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="OO230_003163"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5692821.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="OO230_003164"
     CDS             complement(4712..5647)
                     /locus_tag="OO230_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5692822.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="OO230_003165"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="OO230_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5692823.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="OO230_003166"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="OO230_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5692824.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO230_003167"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="OO230_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5692825.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="OO230_003168"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="OO230_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5692826.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="OO230_003169"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="OO230_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5692827.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="OO230_003170"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="OO230_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5692828.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO230_003171"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="OO230_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5692829.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="OO230_003172"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="OO230_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5692830.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="OO230_003173"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="OO230_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5692831.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3401 a         3299 c         2948 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcacctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaat
//
LOCUS       DAJTPG010000101        12805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTPG010000101 DAJTPG010000000
VERSION     DAJTPG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1175)
                     /locus_tag="OO230_003174"
     CDS             complement(<1..1175)
                     /locus_tag="OO230_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5692832.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="OO230_003175"
     tRNA            complement(1374..1458)
                     /locus_tag="OO230_003175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="OO230_003176"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="OO230_003176"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5692833.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="OO230_003177"
     CDS             2802..4304
                     /locus_tag="OO230_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5692834.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO230_003178"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO230_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5692835.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO230_003179"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO230_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5692836.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="OO230_003180"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="OO230_003180"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5692837.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="OO230_003181"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="OO230_003181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5692838.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="OO230_003182"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="OO230_003182"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5692839.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..>12805)
                     /locus_tag="OO230_003183"
     CDS             complement(11869..>12805)
                     /locus_tag="OO230_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5692840.1"
                     /translation="ILMTVSADMPLVGCVLTLLLLVLLIFMAAKGLRYQALMTSLNGV
                     RFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIISVVLMAIVGIV
                     SIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFLAVAGYI
                     IFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHF
                     MSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYL
                     LGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3241 a         3326 c         3371 g         2867 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcggc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtcaggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatga cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcacaccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttattga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agatt
//
LOCUS       DAJTPG010000102        12695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTPG010000102 DAJTPG010000000
VERSION     DAJTPG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO230_003184"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO230_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5692841.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKQ
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO230_003185"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO230_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5692842.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO230_003186"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO230_003186"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5692843.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO230_003187"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO230_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5692844.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO230_003188"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO230_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5692845.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO230_003189"
     CDS             4025..4615
                     /locus_tag="OO230_003189"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5692846.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO230_003190"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO230_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5692847.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO230_003191"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO230_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5692848.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO230_003192"
     CDS             complement(6551..6658)
                     /locus_tag="OO230_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692849.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO230_003193"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO230_003193"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5692850.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO230_003194"
     CDS             7648..7875
                     /locus_tag="OO230_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5692851.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO230_003195"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO230_003195"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5692852.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO230_003196"
     CDS             complement(9447..10703)
                     /locus_tag="OO230_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5692853.1"
                     /translation="MGSNIHGISCTANNYLKHAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO230_003197"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO230_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5692854.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO230_003198"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO230_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5692855.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQGTTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OO230_003199"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OO230_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5692856.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3031 a         3240 c         3199 g         3225 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tcctgcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggca tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 ggaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAJTPG010000103        12652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTPG010000103 DAJTPG010000000
VERSION     DAJTPG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OO230_003200"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OO230_003200"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5692857.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OO230_003201"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OO230_003201"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5692858.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OO230_003202"
     CDS             3115..3399
                     /locus_tag="OO230_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5692859.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO230_003203"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OO230_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5692860.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO230_003204"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OO230_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5692861.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO230_003205"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OO230_003205"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5692862.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="OO230_003206"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="OO230_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5692863.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="OO230_003207"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="OO230_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5692864.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="OO230_003208"
     CDS             10849..11334
                     /locus_tag="OO230_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5692865.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="OO230_003209"
     CDS             11444..12433
                     /locus_tag="OO230_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5692866.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3337 a         3126 c         2940 g         3249 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgaccccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aa
//
LOCUS       DAJTPG010000104        12631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTPG010000104 DAJTPG010000000
VERSION     DAJTPG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..421)
                     /locus_tag="OO230_003210"
     CDS             complement(<1..421)
                     /locus_tag="OO230_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCR5692867.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYCTKEEAG"
     gene            complement(681..1301)
                     /gene="sodA"
                     /locus_tag="OO230_003211"
     CDS             complement(681..1301)
                     /gene="sodA"
                     /locus_tag="OO230_003211"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5692868.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1586..2620
                     /gene="rhaT"
                     /locus_tag="OO230_003212"
                     /pseudo
     CDS             1586..2620
                     /gene="rhaT"
                     /locus_tag="OO230_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(2617..3483)
                     /gene="rhaR"
                     /locus_tag="OO230_003213"
     CDS             complement(2617..3483)
                     /gene="rhaR"
                     /locus_tag="OO230_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5692869.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(3539..4375)
                     /gene="rhaS"
                     /locus_tag="OO230_003214"
     CDS             complement(3539..4375)
                     /gene="rhaS"
                     /locus_tag="OO230_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5692870.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4663..6132
                     /gene="rhaB"
                     /locus_tag="OO230_003215"
     CDS             4663..6132
                     /gene="rhaB"
                     /locus_tag="OO230_003215"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5692871.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6129..7347
                     /gene="rhaA"
                     /locus_tag="OO230_003216"
                     /pseudo
     CDS             6129..7347
                     /gene="rhaA"
                     /locus_tag="OO230_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            7589..8413
                     /gene="rhaD"
                     /locus_tag="OO230_003217"
                     /pseudo
     CDS             7589..8413
                     /gene="rhaD"
                     /locus_tag="OO230_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            8423..8737
                     /gene="rhaM"
                     /locus_tag="OO230_003218"
     CDS             8423..8737
                     /gene="rhaM"
                     /locus_tag="OO230_003218"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5692872.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9038..9484
                     /locus_tag="OO230_003219"
     CDS             9038..9484
                     /locus_tag="OO230_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5692873.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            9495..10946
                     /locus_tag="OO230_003220"
     CDS             9495..10946
                     /locus_tag="OO230_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5692874.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            10936..12006
                     /locus_tag="OO230_003221"
     CDS             10936..12006
                     /locus_tag="OO230_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5692875.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12006..>12631
                     /locus_tag="OO230_003222"
     CDS             12006..>12631
                     /locus_tag="OO230_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5692876.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         2982 a         3287 c         3344 g         3018 t
ORIGIN      
        1 ccccagcttc ttctttcgta cagtactgct gcatgatgga agcgtaaagt ccgccgttgg
       61 tcatatccat cgccgccacc agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa
      121 caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt
      181 tagttaccac cagcgtaccg gatttacgca gtaccgttcc cgtcgcgctt aattttattg
      241 acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta atcatcccgt
      301 tggtgaatga tccaaaatat ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa
      361 ggaatagcgg gacgagcatc atcccccccg ggattttctc aatcgagcgt tttatctgca
      421 tttccatcac ctgtcggcat atttttaagg gctgatgcgt aattcctcaa tggagcatca
      481 gtgttgatgg aaacgaagat agacattcat ttaatcaata aaagtgatca aaatcacatc
      541 aattgaaacg ctgttttatt tttataggac cattgttttt attttggaat tgatcacaaa
      601 aaaaccaccg ccgttggcga tggttcattt tttaagctga tatgcggcct atacgcctca
      661 ttgcagcagg cggcaaatga ttattttttc gccgcaaaac gtgccgctgc ttcgtcccag
      721 ttcaccacgt tccagaactc tttgatgtag tccggacggc ggttctggaa tttcaggtag
      781 taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc agaaatagct
      841 tcacccatca gcggagaatc ctggttagca gtagaaacca ccgccagttt atcgcctttc
      901 agcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttttc aaattctgct
      961 ttgaagttat caacggagcc gaagtcacgt tcgatagccg ctttcaggtc accctgcagg
     1021 gtggtgcctt ttttcagacc tttccagaac aggctgtggt tagcgtgacc gccagcgttg
     1081 ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat cagctcttca
     1141 accggcaggt tggcaaattc tggcaggctt tccagcgccg cgttggcgtt gtttacgtag
     1201 gtctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg cggttccagg
     1261 gcatcgtaag catacggcag ggatggcagg gtatagctca tattcatctc cagtattgtc
     1321 gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat taaatgatat
     1381 tgaaaatgat tatcaatgcc gtacttttcg taagggtatg gttttgcagg aaaatgcccg
     1441 agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcgac aacggcggca
     1501 acaggcgaaa ggttaatcga cagcacgatt tttacactca tctcgtcgga gatgtgaagc
     1561 gacgaaaaat gatgaggata agaagatgag taacgcgatt acgatgggga tattttggca
     1621 tttgatcggc gcggccagtg cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg
     1681 gtcatgggaa accatgtggt cagtcggtgg gattgtttcg tggattattc tgccgtgggc
     1741 catcagcgcc ctgttactac cggatttctg ggcgtattac agctcgttta gtctctctac
     1801 gctgctgcct gtttttctgt tcggcgctat gtgggggatc ggtaatatca actatggcct
     1861 gaccatgcgt tatctcggca tgtcgatggg aattggcatc gccattggca ttacgttgat
     1921 tgtcggtacg ctgatgacgc caattatcaa cggcaatttc gatgtgctga ttaacaccga
     1981 aggcggacgc atgacgttac tcggcgttct ggtggcgctg attggcgtag ggattgtaac
     2041 tcgagccggg cagttgaaag agcgcaagat gggcattaaa gccgaagagt tcaatctgaa
     2101 aaaagggctg gtgctggcgg tgatgtgcgg cattttctct gccgggatgt cctttgcgat
     2161 gaacgccgca aaaccgatgc atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt
     2221 tgctctgcca agctatgttg tcatcatggg cggcggcgcg atcattaacc tcggtttctg
     2281 ttttattcgt ctggcaaaag tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa
     2341 accgctgatc attcacaatg tgttactctc ggcactgggc gggttgatgt ggtatctgca
     2401 attctttttc tatgcctagg gccacgcccg cattccggcg cagtatgact acatcagttg
     2461 gatgctgcat atgagtttct atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga
     2521 gtggaacaat acaggacgcc gtccggtaac ggtgttgagc ctcggttgtg tggtgattat
     2581 tgtcgccgct aacatcgtcg gcatcggcat ggcgaattaa tctttctgcg aattgagatg
     2641 acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa aatagttact
     2701 atcttcaaag ccacattcgg tcgaaatatc actgattaac aggcggctat gctggagaag
     2761 atattgcgca tgacacactc tgacctgccg cagatattga ttgatggtca ttccagtctg
     2821 ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac aaaatttatc
     2881 cagcgcaaag ggacttttca ggctagccgc cagccgggta atcagcttat ccagcaacgt
     2941 ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca gcaacatcac
     3001 caactgcccg aacagcaact cagccatttc gttagcaaac ggcacatgct gactactttc
     3061 atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca
     3121 gtgtggttgc cctgcgctgg cgctaaatcc cggaatcgct ccctgccagt caagattcag
     3181 cttcagccgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa cggaagcgta
     3241 ggagtgttta tcgtcagcat gaatataaaa gagatcgcca cgggtaatgc gataaggacg
     3301 atcgttgagt acatgcagac cattaccgcg ccagacaatc accagctcac aaaaatcatg
     3361 tgtatgttcg gcaaagacat cttgcggata acggtcagcc acagcgactg cctgctggtc
     3421 gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg ctacctcggc
     3481 cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt
     3541 attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg tgaccagtta aactctcggc
     3601 gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca gcgataggcg atgtcagtga
     3661 cgctggcctc gctgtggcgt agtagatgtc gggctttcat cagtcgcagg cggttcaggt
     3721 atcgctgagg cgtcagtccc gtttgctgct taagctgccg atgtagcgta cgcagtgaaa
     3781 gagaaaattg atccgccacg gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc
     3841 aggccagaag caagttgaga cgtgatgcgc tgttttccag gttctcctgc aaactgcttt
     3901 tacgcagcaa gagcagtaat tgcataaaca agatctcgcg actggcggtc gagggtaaat
     3961 cattttcccc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc tgcaatacgc
     4021 tgtggttaac gcgccagtga gacggatact gcccatccag ctcttgtggc agcaactgat
     4081 tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacattg gtcagacaca
     4141 gattatcggt atgttcatac agatgccgat catgatcgcg tacgaaacag accgtgccac
     4201 cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt tcgacaatca
     4261 caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc cggggttcta
     4321 tcgccacgga cgcgttacaa gacggaaaaa aatccacact atgtaatacg gtcatactgg
     4381 cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct taccttaaat
     4441 tttcgacgga aaaccacgta aaaaacgtcg atttttcaag atacagcgtg aattttcagg
     4501 aaatgcggtg agcatcacat caccacaatt cagcaaattg tgaacatcat cacgttcatc
     4561 tttccctggt tgccaatggc ccattttcct gtcagtaacg agaaggtcgc gaattcaggc
     4621 gctttttaga ctggtcgtaa tgaaattcag caggatcaca ttatgacctt tcgcaattgt
     4681 gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa
     4741 tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca atgggctgca tagccaaaac
     4801 ggttatgtca cctgggatgt ggatagcctg gaaagtgcca ttcgccttgg attaaacaag
     4861 gtgtgcgagg aagggattcg tatcgatagc attgggattg atacctgggg cgtggacttt
     4921 gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc
     4981 accaatggcc tgatgccgca ggcacaacag caactcggca aacgcgatat ttatcagcgt
     5041 agcggcatcc agtttctacc cttcaatacg ctttatcagt tgcgtgcgct gacggagcaa
     5101 caacctgaac ttattccaca cattgctcac gctctgctga tgccggatta cttcagttat
     5161 cgcctgaccg gcaagatgaa ctgggaatat accaatgcca ccaccacaca actggtcaat
     5221 atcaatagcg acgactggga cgagtcgcta ctggcgtgga gcggggccaa caaagcctgg
     5281 tttggtcgcc cgacgcatcc gggtaatgtc attgggcact ggatttgccc gcagggtaat
     5341 gagattccgg tggtcgccgt tgccagccat gataccgcca gcgcggttat cgcctcgccg
     5401 ttaaacggtt cacgcgccgc ttatctctct tctggcacct ggtcattgat gggcttcgaa
     5461 agccagacgc catttaccaa tgacacggcg ctggcagcca acatcaccaa tgaaggcggg
     5521 gcggaaggtc gctatcgggt gctgaaaaat attatgggct tatggctgct tcagcgagtg
     5581 cttcaggaac ggcaaatcaa cgatctcccg gcgcttatcg ccgcgacaca ggcacttccg
     5641 gcctgccgct tcatcatcaa tcccaatgac gatcgcttta ttaatcctga cgagatgtgc
     5701 agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga tcccgggaag tgatgctgaa
     5761 ctggcgcgct gtattttcga cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg
     5821 gcgcagctgc gcggtgaaga tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac
     5881 acgctgctca accagctatg cgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt
     5941 gaagcctcaa cgctcggcaa tatcggcatc gagttaatga cgctggatga actcaacaat
     6001 gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc tgaccacctt tacccctaat
     6061 cctgacagtg aaattgccca ctatgtggcg cagattcact ctacacgaca gacaaaggag
     6121 ctttgcgcat gaccactcaa ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg
     6181 cggtggggat tgatgtcgag gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc
     6241 actgctggca gggcgatgat gtttccggtt ttgaaaaccc ggaaggttcg ctgaccgggg
     6301 ggattcaggc cacaggcaat tatccgggca aagcgcgtaa tgccagtgag ctacgtgccg
     6361 atctggaaca ggctatgcgg ctgattccgg ggccgaaacg gcttaattta catgccatct
     6421 atctggaatc agatacgcca gtctcgcgcg accagatcaa accagagcac ttcaaaaact
     6481 gggttgaatg ggcgaaagcc aatcagctcg gtctggattt taacccctcc tgcttttcgc
     6541 atccgctaag cgccgatggc tttacgctct cgcatcccga cgacagcatt cgccagttct
     6601 ggattgatca ctgcaaagcc agccgtcgcg tttcggccta ttttggcgag caactcggca
     6661 caccatcggt gatgaacatc tggatcccgg atggtatgaa agatatcacc gttgaccgtc
     6721 tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt gatcagcgag aagctaaacc
     6781 ctgcgcacca tatcgacgcc gttgagagca aattgtttgg cattggcgca gagagctaca
     6841 cggttggctc caatgagttt tacatggggt atgccaccag ccgccagact gcgctgtgcc
     6901 tggacgccgg gcacttccac ccgactgaag taatttccga caagatttcc gccgccatgc
     6961 tgtatgtacc gcagttgctg ctgcacgtca gccgtccggt tcgctgggac agcgatcacg
     7021 tagtgctgct ggatgatgaa acccaggcaa ttgccagtga gattgtgcgt cacgatctgt
     7081 ttgaccgggt gcatatcggc cttgacttct tcgatgcctc tatcaaccgc attgccgcgt
     7141 gcgttgctgg aacctaccgc tgagctgcgc aagctggaag cggcgggcga ttacactgcg
     7201 cgtctggcac tgctggaaga gcagaaatcg ttgccgcggc aggcggtctg ggaaatgtat
     7261 tgccaacatc acgatacgcc agcaggtagc gaatggctgg agagcgtgcg ggcttatgag
     7321 aaagcgattt tgagccagcg tgggtaaaca ctgccggatg cggcacgagc gccttatccg
     7381 gcctacgggt cgaatgtcca tgcgggctgt caccctcacc ccggccctct ccctgaaagg
     7441 gcgagggaga aaaacatagc ctcgtgcggc aacggagctg aacaccgctc cggacagtcc
     7501 cctcgcccct acggggagag ggttagggtg aggggaacaa gccgcaccat gtctaaacaa
     7561 ggcaacctaa tttacaggaa cacagaacat gcaaaacatt acccagtcct ggtttgtcca
     7621 gggaatgatc aaagccacca ccgatgccta gctgaaaggc tgggacgagc gcaacggcgg
     7681 caacctgacg ctgcgcctgg atgacgccga tatcgcgctt tatcacgaca acttccaccc
     7741 acaaccgcgc tatattccgc tcagccaacc catgccttta ctggcaaata cgccgtttat
     7801 tgtcaccggc tccggcaaat tcttccgtaa cgtccagctc gatcctgcag ctaacttagg
     7861 cgtcgtaaaa gtcgacagcg atggcgcggg ctaccatatt ctctgggggt taaccaacga
     7921 agccgttcca acttctgaac ttccggctca ctttctctcc cactgcgagc gcattaaagc
     7981 caccaacggt aaagatcggg tgatcatgca ctgccacgcc accaacctga tcgccctcac
     8041 ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa ctgtgggaag gcagcaccga
     8101 atgtctggta gtattcccgg atggcgttgg cattctgccg tggatggtgc ccggcacgga
     8161 cgaaatcggc caggcgaccg cacaagagat gcaaaaacat tcgctggtgt tgtggccctt
     8221 ccacggtgtg ttcggttccg gaccgacgct ggatgaagcc tttggtttaa tcgacaccgc
     8281 agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga aacagaccat
     8341 cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc gttacaccac tagccagtgc
     8401 gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg taaaccccga
     8461 cgcccacgaa gagtatcagc gtcggcataa tcccatctgg ccggaactgg aagcagtgtt
     8521 gaaatctcac ggtgtgcata actacgccat ttacctcgac aaagcgcgta atctgctgtt
     8581 tgccatggta gagattgaat ctgaagaacg gtggaatgcg gtagccagta ctgatgtttg
     8641 ccaacgttgg tggaaatata tgaccgatgt tatgcccgct aacccagata acagcccggt
     8701 gagtagcgag ctgcaagaag tgttttacct gccgtaattg cccaatgtgc cggagatgcc
     8761 tccggcacaa tattttaaat ttcgatttga aaaattaaac atattacatc tcccggtaag
     8821 ttatatttcc ctgatacatt gtgagtaaat cacaaaaata atgaataacc cattaatgat
     8881 tcatgtggtt tatttaaata accattatgt gcattactcc gcaaatctga cctttcactc
     8941 tgtctcaaat tgtcattgtt taccgacata tcgcctctca tcacttaatc gctccaccat
     9001 tacaattgat taaaatatat tttatggagt ccgattaatg gcagctctta ctgcaagctg
     9061 tattgacctg aatattcagg gcaatggcgc ttattccgtt ctgaagcagt tggcgacaat
     9121 agcgttacaa aacggtttta tcaccgactc acaccagttc ctgcaaaccc tgctgctgcg
     9181 cgaaaaaatg cactctactg gatttggttc cggtgtcgca gtgccgcacg gtaaaagcgc
     9241 ctgcgttaaa caaccgttcg tattattcgc ccgcaaagcg caggctattg actggaaagc
     9301 cagcgatggc gaagacgtca attgctggat ctgcctcggc gtgccgcaaa gcggcgaaga
     9361 ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt attcaccagg aatttattca
     9421 tcaactgcaa cagggcgata ccgaccaggt gctggccttg ttaaatcaaa ccctcagctc
     9481 ataaggaagt ggcgatggag tcatccttac gtattgtcgc gatcaccaac tgccccgccg
     9541 ggatcgctca cacctacatg gtggcggaag ccctggaaca gaaagcccgt tctctcggcc
     9601 ataccataaa agtggaaact caagggtcca gtggcgttga aaaccgctta tccagcgaag
     9661 agattgccgc tgccgattac gtcattcttg ctaccgggcg tggcctgagc ggtgatgatc
     9721 gcgcgcggtt tgccgggaag aaagtttatg agattgccat ctccctggcg ttgaaaaata
     9781 tcgaccagat tttcagcgaa ttaccgacaa actcgcagct ttttgccgca gatagcggcg
     9841 tgaagcttgg caaacaggaa gtgcaaagcg gcagcgtgat gagccacctg atggcgggcg
     9901 tctctgcggc gctgccgttt gtcatcggcg gtggtatcct ggtggcgctt gccaacatgc
     9961 tggtgcagtt cggtttgccg tataccgata tgtcgaaagg cgcgccgtcg tttacctggg
    10021 tagttgaatc catcggttac cttggcttca cctttatgat ccccatcatg ggcgcttata
    10081 ttgcctcgtc gattgccgat aaaccggctt tcgctccggc gtttctggtt tgctatctgg
    10141 cgaacgacaa agcgctgctc ggcacccagt cgggtgcggg attcctcggc gcggtggtgc
    10201 tggggctggc gattggctat ttcgtcttct ggttccgcaa agtgcgcctt ggcaaagcat
    10261 tacaaccgct gctcggttca atgctgatcc cgttcgttac tctgctggtt ttcggcgtgc
    10321 tcacttacta cgttatcggg ccggtgatgt ccgacctcat gggcgggctg ctccacttcc
    10381 tgaataccat tcctccatca atgaagtttg cggcagcgtt tctggtcggt gcgatgctgg
    10441 cgttcgatat gggcggcccc atcaataaaa ccgcctggtt cttctgcttc tcactgctgg
    10501 aaaaacacat ttatgactgg tacgccatcg tcggcgttgt cgcgttgatg ccgcccgttg
    10561 cggcgggtct tgcgacgttc attgcgccca aactgtttac ccgacaggaa aaagaggctg
    10621 ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga accggctata ccttacgctc
    10681 tggcagctcc gttgccgatg attaccgcca atacgctggc gggcggcatt accggcgtgc
    10741 tggtgatcgc tttcggaatc aaacgtctgg cacctggttt gggtatcttt gacccgctga
    10801 tcggcctgat gtcgccggta ggttcgtttt atctggtgct ggcgattggc ctcgcgctga
    10861 acatttcatt cattatcgtc ctgaaaggat tgtggttacg ccgcaaagcg aaagccgcgc
    10921 aacaggagct tgtccatgaa cattgagtta ctgcaacagt tgtgcgaagc cagcgccgtt
    10981 agcggcgatg aacaggaagt tcgcgacatc ctgataaaca cgctggaacc ttgcgttaat
    11041 gaaatcacct ttgatggtct gggcagcttt gttgcccgta agggaaataa aggtccaaaa
    11101 gttgccgttg tcgggcatat ggatgaagtc ggctttatgg tcacccacat cgacgagagc
    11161 ggttttctgc gtttcaccac cattggtggc tggtggaatc agtcgatgct caaccatcgg
    11221 gtaaccatac gcacacacaa gggagtgaaa atccctggtg tgattggttt cgtcgcgcct
    11281 catgcgttaa cggaaaagca aaggcaacag ccgctgtcat ttgatgagat gttcattgat
    11341 attggcgcaa acagtcgcga tgaggtggaa aaacgcggtg tggcgatggg cgattttatt
    11401 agcccggaag ccaattttgc ctgctggggc gaagataaag tggtcggcaa ggcgctggat
    11461 aaccgcatcg gctgcgcaat gatggctgaa ctattgcaga cggtgaataa tcccgaaatt
    11521 acgctgtatg gcgtcggcag cgtggaagaa gaagttgggc tacgcggggc acaaacctcg
    11581 gcggaacaca ttaaaccgga tgtagtgatc gtgctggata ccgctgtcgc aggcgatgtt
    11641 ccgggcattg ataacattaa atacccgctg aaactgggcc aggggccggg gctgatgctg
    11701 tttgacaagc gctacttccc caaccagaaa ctggtagcag cgttaaaaaa ctgtgccgca
    11761 cataacgatt taccgctgca attttccacc atgaaaaccg gagcgacgga tggcgggcgc
    11821 tacaacgtga tgggcggcgg gcgtccggtt gttgcgctgt gcctgccaac ccgctatctg
    11881 cacgctaaca gcggcatgat ttcaaaagcc gattacgatg ctctgctcac gttgatacgg
    11941 ggttttctga cgaccttaac cgcggagaaa gtcaacgcgt ttagccagtt ccgtcaggtg
    12001 gattaatgct aaacgaacgc cagttaaaga ttgtcgatct gctggagcaa cagccgcgca
    12061 cgcctggtga gctggcgcaa cagactggcg tttcaggcag gaccatcctg cgtgatattg
    12121 actatctcaa ctttaccctt aacggcaaag cccgcatttt cgccagtggc agtgcgggct
    12181 atcagctgga aatcttcgag cgccgcagct tttttcagtt gctgcaaaag cacgataacg
    12241 acgatcggct gctggcgctg ttattactga atactttcac tccccgtgcg caactcgcct
    12301 cggcgcttaa tttgccagaa acgtgggtag cagagcgtct gccccagtta aaacagcgtt
    12361 atgaacgcac ttgttgcctg gccagccgcc ctggtttggg ccatttcatt gatgagacag
    12421 aagagaaact cgttatcttg ctggcgaact tgctgcgcaa agatccgttt ttaattccgc
    12481 tggcgggcat aacacgagac aaccttcagc atttatccac ggcctgcgac aaccaacacc
    12541 gctggccgct catacagggt gattatctct ccagcctgat tctggcgatt tacgccctgc
    12601 gtaatcaact gaccgatgag tggccgcaat a
//
LOCUS       DAJTPG010000105        12615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTPG010000105 DAJTPG010000000
VERSION     DAJTPG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..1040
                     /locus_tag="OO230_003223"
     CDS             <1..1040
                     /locus_tag="OO230_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5692877.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OO230_003224"
     CDS             1041..3206
                     /locus_tag="OO230_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5692878.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3220..4131
                     /locus_tag="OO230_003225"
     CDS             3220..4131
                     /locus_tag="OO230_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5692879.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OO230_003226"
     CDS             4131..5426
                     /locus_tag="OO230_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5692880.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OO230_003227"
     CDS             5471..5701
                     /locus_tag="OO230_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692881.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OO230_003228"
     CDS             5679..6179
                     /locus_tag="OO230_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5692882.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OO230_003229"
     CDS             6179..7597
                     /locus_tag="OO230_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5692883.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OO230_003230"
     CDS             7597..8445
                     /locus_tag="OO230_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692884.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OO230_003231"
     CDS             8445..8900
                     /locus_tag="OO230_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5692885.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OO230_003232"
     CDS             8903..9595
                     /locus_tag="OO230_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5692886.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OO230_003233"
     CDS             9605..10936
                     /locus_tag="OO230_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5692887.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10937..>12615
                     /locus_tag="OO230_003234"
     CDS             10937..>12615
                     /locus_tag="OO230_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5692888.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDL"
BASE COUNT         3392 a         3123 c         3325 g         2775 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gaagcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 ttccaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac
    12181 agtagcagca caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc
    12241 tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca
    12301 cttttccggg aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc
    12361 tgctctacag gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat
    12421 aattgatcgc gtttccggtg caaaagatgc tcttggtatg agcgacaagt ttattgatac
    12481 ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac cagctttatc gcaatgttga
    12541 aaaggcgatg cctgcaggtg caaaaattga tgcgccatca acaaggtcaa tgctcaaaca
    12601 ggtggcagaa gatct
//
LOCUS       DAJTPG010000106        12589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTPG010000106 DAJTPG010000000
VERSION     DAJTPG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(85..804)
                     /locus_tag="OO230_003235"
                     /pseudo
     CDS             complement(85..804)
                     /locus_tag="OO230_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(820..1152)
                     /gene="cusF"
                     /locus_tag="OO230_003236"
     CDS             complement(820..1152)
                     /gene="cusF"
                     /locus_tag="OO230_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5692889.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1176..2558)
                     /gene="cusC"
                     /locus_tag="OO230_003237"
     CDS             complement(1176..2558)
                     /gene="cusC"
                     /locus_tag="OO230_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5692890.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2715..3398
                     /gene="cusR"
                     /locus_tag="OO230_003238"
     CDS             2715..3398
                     /gene="cusR"
                     /locus_tag="OO230_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5692891.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3388..4830
                     /gene="cusS"
                     /locus_tag="OO230_003239"
     CDS             3388..4830
                     /gene="cusS"
                     /locus_tag="OO230_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5692892.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            4980..7217
                     /gene="nrfB"
                     /locus_tag="OO230_003240"
     CDS             4980..7217
                     /gene="nrfB"
                     /locus_tag="OO230_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5692893.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7204..10172
                     /gene="nfrA"
                     /locus_tag="OO230_003241"
                     /pseudo
     CDS             7204..10172
                     /gene="nfrA"
                     /locus_tag="OO230_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10173..11063
                     /locus_tag="OO230_003242"
     CDS             10173..11063
                     /locus_tag="OO230_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5692894.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11246..12007
                     /gene="envY"
                     /locus_tag="OO230_003243"
     CDS             11246..12007
                     /gene="envY"
                     /locus_tag="OO230_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5692895.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12053..12129)
                     /locus_tag="OO230_003244"
     tRNA            complement(12053..12129)
                     /locus_tag="OO230_003244"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12093..12095),aa:Arg,seq:tct)
     gene            12372..>12589
                     /locus_tag="OO230_003245"
     CDS             12372..>12589
                     /locus_tag="OO230_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5692896.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRVML"
BASE COUNT         3066 a         3276 c         3206 g         3041 t
ORIGIN      
        1 aaaggttgtt tttcgagtta taacagacag ttaattaact acagtggaca atgctagaca
       61 atgacagaca cagataagca acctaccttc ctctttcgcg accacgttat ctttggcgat
      121 attcattccc gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg ctttgagcgt
      181 aaagcgagtc tggatttttt gcgtggcgat caggtggcga atatccgcct ccggcattcc
      241 cgccagtcgc agccgctcaa gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg
      301 caacagtaaa tactcactct gcgcttccac ccagtcggga atggtcaggt cgagaagcgg
      361 tgtgcccttt tgtactttat cgcccacggt aagcggatac accttgtcga taaacccggc
      421 agcgcgagcc tgcacaatgg catactgata ctcgttgtaa ctgacattcg tcgggaaact
      481 ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt
      541 ctgagtcggg tcaatgcgca caccagacgc agaactctct tcatcggcat atttcggcac
      601 cagatccata tccataaacg gcgatttacc tggtttatcg aaccgcgtat tgggatacat
      661 cgggtcgtac cagaataaga ttttacgttc tgcggtcggc attttttctg cgtgcggttc
      721 cgcctggtca aaccaggtaa aacctgccgc tgaaataata ccgcccgcga tcatgctgcc
      781 gataataagc gcgatttttt tcatctcatt aaacctgggt tactggctga ctttaatatc
      841 ctgtaataaa gaaaggttgc cctgctggac aaaattaaac gccactttgt cgccggtttt
      901 aatttcactc attttcgtct gcggggtgat ggtaaagcgc atggtcatct ccggccagtt
      961 gacggcagca atcggatcgt gatggatggt gatttttttg ctttccagat caacaccttt
     1021 taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg ctcatggttt catgatgatg
     1081 ttcgttagcc tgggcattaa agccaataac ggtaaacaga ctgaacattg cgacttgcag
     1141 tgcttttttc atttattctc tcctggagtt aaaaattatt gctgccaacc gccccctaag
     1201 gcggtataca aataaatttc gttaacctga cgggcataat tcaggtcaag taaagtttgt
     1261 cgggttgcaa ataaagaacg ctcggcatcc agcacttcca gataacttac cgcgccgtgc
     1321 tgatataatg cccgcgcccg ttgcagggta atttgcagcg acgccagata acgctgctgg
     1381 gcgctgattt gatcgtccag gctttgacgt aatgccagcg catctgccac ctctttaaag
     1441 gcgttctgga ttttctgttc ataattcacc accgactgct gctggcgaat ttcggcgata
     1501 tccagattgg cctggttgcg tccggcatta aaaatgggga tctcaatttt aggaataaaa
     1561 ttccacatcc cgctgctagc gttaaataat gacgacagat cgctgctggc ggttgatatt
     1621 ccgctggtca ggctgataga cgggaaaaat gccgcacgcg cagcaccaat attagcatta
     1681 gccgccatta acgcgtgttc agcttccata atatccgggc gctgcaataa gatttgcgac
     1741 gacaagcccg ccggtaattt aacgctttgc aggctgccgc tgtttactgt ctgcgcttgc
     1801 ggtagcttgc cgtagcttcc caataacagt tgcaatgcat tattcgcctg cgccagttcc
     1861 ccctgacgtt tagcgatgtc actgcgggta ctttctatca ccccgcgagc ctgttccagc
     1921 gccagaacat tgctgctacc ggtcaacagt tgtttttcga caaacgcata tgactgctga
     1981 taattacgca gcgtttcttc ggctatttgc aattgcgcat acgccagctg ctgattgaaa
     2041 tagctttgcg cgacattaga aaccagcaaa atatgcaccg cgcgctgggc ttcctcagtg
     2101 gctaaataat tttggcgctc ggcttcgctc atgttcttta agcgaccgaa aaaatcgaga
     2161 tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg tggctgaatc gcctttaaga
     2221 ttgccgctcc agctgccgct gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc
     2281 agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg ccatgcgcaa atcccggtta
     2341 ttcaccagcg cctcgctaat cagcgtcttc acctgattat caacaaaaaa ggtgcgccag
     2401 cccacgttct ggtagttatc cgctgcgtta accaggccgt tctggctgag tgagaactgc
     2461 tgcggtacgg gcattaccgg acgctgataa tccggtgcca gtgaacaacc agttagcgca
     2521 agggccacac aaaatggcag aagtttacaa ggagacatag gctcataatt tctggtgatt
     2581 ttatgtcgcc aactttactc gccaggctct gattttccgg tgacaggaaa atgacaaaat
     2641 tgtcattttg ccaataagcg attgccatct gatcccgcta ctctagaatt gcccgggcaa
     2701 catgcggagg aaatatgaaa ctgttgattg tcgaagatga aaagaaaacc ggagaatact
     2761 tgaccaaagg gttaaccgaa gccggttttg tggtcgattt ggccgacaac gggctgaatg
     2821 gctaccatct ggcgatgacc ggtgattatg acctgataat cctcgatatt atgctgccag
     2881 acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc caataaaggg atgccgattc
     2941 tgttgcttac cgcgcttggc accattgaac atcgcgtcaa ggggctggag ttgggggcag
     3001 atgactacct ggtgaaaccg ttcgcttttg ctgaactgct ggcgcgggtg cgcactctcc
     3061 tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca ggttgccgat ctgatggtcg
     3121 atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat cactctgacc agtaaagaat
     3181 ttactctgct ggagtttttt ctccgccatc agggcgaagt gctgccccgc tcgcttatcg
     3241 cctcgcaggt atgggacatg aatttcgaca gcgacactaa cgccattgat gtggcggtga
     3301 agctgctacg cggcaaaatc gacaacgact ttgagccgaa gctgattcaa accgtgcgcg
     3361 gcgtgggtta catgcttgag gtgccggatg gtcagtaagc catttcagcg cccgttttcg
     3421 ctggcaaccc gcctgacctt ttttatcagc caggccacca tcgcggcgtt tttcgccttt
     3481 gcatggatca taatccactc agtaaaagtg cattttgccg agcaggacat taatgattta
     3541 aaagagatta gcgccaccct tgaacgggta ctaaatcacc ctgacgaaac gcaagcccga
     3601 cgcttaatga cgctggaaga tatcgtcagt ggttattcca acgtgttgat ttccctggca
     3661 gatagccacg gcaaaacggt gtatcactcc cccggtgcgc ctgatatccg cgagtttgca
     3721 cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg tgtatctcct ttccggcccg
     3781 acgattatga tgccaggcca cggtcacggg catatggaac acagcaactg gcggatgatt
     3841 aacttgccgg ttggcccgtt ggtggacggc aaaccgattt atacgctcta catcgcgctg
     3901 tcgatcgatt ttcatcttca ttacataaat gatttgatga ataaacttat tatgaccgca
     3961 tcggtaatca gcatcctgat cgtctttatc gtgctgttgg cggtacataa aggtcacgcg
     4021 ccgatccgca gcgtcagccg tcaaatccag aatatcacct cgaaagatct cgacgttcgc
     4081 cttgacccgc agaccgtgcc gattgagctg gaacagctgg tactgtcgtt caaccatatg
     4141 atcgagcgta ttgaggatgt ctttactcgc cagtccaatt tctcagcgga tatcgcccac
     4201 gaaattcgca caccaattac taatctcatt acgcaaacgg aaatcgccct cagccagtcg
     4261 cgcagccaga aggagctgga agatgtgctc tactctaatc tcgaagagct gacgcgaatg
     4321 gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg ataacaacca gctaatcccc
     4381 gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag tgttcgattt tttcgaggcg
     4441 ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg acaaacgtca ggttgcgggc
     4501 gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt ctaatgccct tcgttatacg
     4561 ccaaccagag agacaattgt agtacgctgt cagacggtcg atcaccaggt gcaagtttcc
     4621 gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac cgcgattgtt tgaccgtttc
     4681 tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta gcggtattgg gctggcgata
     4741 gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg taacgtcaga tgcgcggggg
     4801 acaaggtttg ttatcacatt acccgcttaa ttattcagca aacggcaact tttataacca
     4861 gtgtaaaaat aacgtgccgc aatagtcata catattaatg gtaaaaagct gtcacaattc
     4921 ataaaaaacc ttaatatacg ccaccctaaa cataaccagc gttaatgtaa ggtttttgtg
     4981 tggactggct tcttgatgtt tttgctacct ggctctacgg cttaaaagta atcgcgataa
     5041 cgttagcggt catcatgttc atcagcgggc tggacgattt ttttattgat gtcgtctact
     5101 gggtacgccg cattaaacgc aagttgagtg tttatcgccg ctacccgcga atgagttacc
     5161 gcgaactgta taaaccagat gaaaaaccgt tagcgattat ggttccggcg tggaatgaaa
     5221 cgggcgtcat cggcaatatg gccgagctgg cggcgaccac gctcgactac gaaaactatc
     5281 atatctttgt tggcacctac cccaacgacc ccgatactca acgtgatgtt gacgaagtgt
     5341 gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc accagcaaag
     5401 ccgactgcct gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc gccaatttcg
     5461 cttttgctgg ttttattctg catgacgccg aagatgtgat ttcaccgatg gaattgcgtc
     5521 tgttcaacta tctggtcgag cgtaaagatc tgattcagat cccagtgtat ccgtttgaac
     5581 gcgaatggac gcacttcacc agcatgactt acattgatga gttttcagaa ctgcatggca
     5641 aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc gtcggcacct
     5701 gtttcagccg ccgcgccgtg accgcactgt tagctgacgg taacggtatt gctttcgacg
     5761 tgcagagtct tactgaagat tacgacattg gcttccgcct gaaagaaaaa ggtatgacgg
     5821 aaatttttgt ccgttttccg gtggtggacg aagccaaaga acgcgagcag cgtaaatttt
     5881 tacagcacgc acggacgtca aacatgatct gcgtgcgcga atatttcccc gatacctttt
     5941 cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat tgttttccag ggctttaaaa
     6001 cccataaatg gacctccagc ctgacgctga actactttct ctggcgcgac cgcaaagggg
     6061 caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat gatccagctt ttgctgttgc
     6121 tggcgtatga aagtttgtgg cccgatgcct ggcatttcct ttctattttt agcggcagcg
     6181 catggttaat gaccctgctg tggctaaact ttggcttgat ggttaaccgc atcgtgcagc
     6241 gagtgatttt cgtcactggc tactacggcc tcacgcaggg cctgctttcc gtcctgcgtc
     6301 ttttctgggg caacctgatt aactttatgg ctaactggcg cgtgctgaaa caggttcttc
     6361 agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac gcatgacttc cccagcgtca
     6421 ctggcgatac ccgctcgttg cgcccgttag gtcaaattct gctggaaaat caggtcatca
     6481 ctgaagaaca actcgataca gcactgcgta atcgcgtcga aggtctacgc ctgggcggtt
     6541 caatgctgat gcaggggctg attagcgccg agcagctggc acaggcgctg gcagagcaaa
     6601 acggcgtggc gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg attgccgaaa
     6661 tgccagcctc cgtagcgctg cattatgcag tactgccgct gcgtctggaa aatgatgagt
     6721 taattgtcgg cagtgaagat ggtattgacc cggtttcgct ggcggccctg acgcgtaaag
     6781 tcggacgcaa agtgcgttac gtcattgttc tgcggggaca aattgtcaca gggttacgcc
     6841 actggtatgc acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat gcggttcagc
     6901 atcagtggct cacggaacag cagaccggtg aaatctggcg gcaatatgtg ccgcatcagt
     6961 tcctgttcgc cgaaatactg accacgctcg gtcatattaa tcgttcagca attaacgtgt
     7021 tgttattgcg ccatgaacgc agttctctgc cgctcggcaa gtttttggtc accgaaggcg
     7081 ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta caagtttcga
     7141 tgcaatcact attactcaaa gcaggtttaa acacagaaca ggttgcgcaa ctggagtccg
     7201 aaaatgaagg agaataacct taatcgcgtc atcggatggt ctggtttact gctgacgtct
     7261 ttattgagta ccagcgcact cgcagacaat atcggcacca gcgcagaaga gctggggctg
     7321 agcgattatc gccattttgt tatttatccc cgtctcgata aggcgctgaa ggcacagaaa
     7381 aataacgacg aagcaaccgc catccgcgaa tttgaatata tacaccaaca ggtgccggat
     7441 aatattccgc tgactttata ccttgcggaa gcctatcgtc attttggtca tgatgaccgg
     7501 gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag gagatgcccg acttgagcgc
     7561 agtctggcgg ctattccagt tgaagtgaaa agcgttacga ctgttgaaga actgcttgcc
     7621 cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc gcagtgaagt cgggcagaat
     7681 gccctgcggc tggcacaatt acctgtcgcc agagcgcaac tgaacgatgc gacgtttgct
     7741 gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat ctacctgaaa
     7801 caatgatccc aggcagatac gctatacaat gaagcacgcc agcagaacac attaagcgcg
     7861 gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc agctggacga tcggatcctg
     7921 gcactgcaat cacaggggat cttcaccgat cctcagtcat atattactta cgcgaccgcg
     7981 ctggcttatc gtggcgaaaa agcacgcctc cagcattatc tcattgaaaa taagccgctg
     8041 tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt tatctaaata cagcgccaac
     8101 cccgttcagg cattggcgaa ttatacggtg cagtttgccg ataaccgcca gtatgttgtt
     8161 ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg acgcagcgca aaaactgctc
     8221 gccaccctcc ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt ggcgacccgt
     8281 aacaaggctg aatctctgcg tctggcacga ttgctgtatc agcaagaacc gacaaatctt
     8341 ccccgcctgg atcaactgac ctggcaactg atgcagaacg agcagtcacg cgaagctgcc
     8401 gatttattgc tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca gactttaatg
     8461 gcgcgactgg cgtctctgct ggaaagtcat ccttacctag caacgccggc gaaggtggcg
     8521 attttatcga aacccttacc gctggcggag caacgtcagt ggcaaagtca gttgccgggt
     8581 attgcagata attgcccggc aatagttcgc ttgctgggcg atatgtcgcc ttcctacgat
     8641 gctgccgctt ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg tgtggcgttg
     8701 tatgcatggc ttcaggccga acaacgacaa ccgagcgcct ggcaacatcg tgcggtagcc
     8761 tatcaggcgt atcaggttga ggactacgcc accgcactgg cggcctggca gaaaatcagt
     8821 cttcacgaca tgagcaatga tgatctgctc gccgccgcca acaccgctca ggcagcggga
     8881 aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac aacgtgagct gggaaacaat
     8941 gctcaatact ggtggctgca tgcgcgacgt tatatcccag ctcagccaga actcgcactg
     9001 aacgatctca cgcgctcaat caatattgcc ccttctgcca actcttacgt tgcgcgggcg
     9061 acaatttatc gccaacgtca taatgttccg gccgcggtga gtgatttgcg cgccgcgctg
     9121 gaactggaac caaataatag caacacccag gcagcgcttg gttacgcctt gtgggatagc
     9181 ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct tccggacgat
     9241 ccggcactga tccgacaact ggcctacgtg aaccagcgtc tggatgacat gcctgcgacg
     9301 cagcactacg cccggctcgt gattgatgac attgataatc aggcataacc ccactgaccc
     9361 cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc ggtcgccgct
     9421 ggacgttcag tttcgattct tccatcggct tgcgttccgg cgcaatgagt accgctaaca
     9481 ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag ctacggacaa ctggaagccg
     9541 agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt tatagccgcg
     9601 tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg atgtccggca
     9661 ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc gaacagcagt
     9721 tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc gcctcattct
     9781 ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg tttgcccaaa
     9841 acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg acggcagatt
     9901 atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct tacgctcacg
     9961 ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc ggtgtcgggg
    10021 tccgctggaa tatttggacc ggcgagacgc actacgacgc ctggccgcac aaagtcagtc
    10081 tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga gagcgcaaca
    10141 acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc gtattgctga
    10201 cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg caaccacaaa
    10261 accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag ttacgttttc
    10321 aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattt acccagccag
    10381 aacagcgcac gttattgttt aagcgtgccg cagctgcgca acagactggc ctgaagctga
    10441 ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg tctgcagcgc
    10501 tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga ttatggagcg
    10561 ccgcgcctgg cataacgccg gatggctggt acatcagcgc ggaaattgac gacctgaact
    10621 ggcgcagcga agccgcccgt caacctttgc taacatggtt aaacaacgcg cagcggctga
    10681 ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga aacatgtcgc
    10741 ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggagttaat gtctgggtac
    10801 aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat ctacaggcca
    10861 gcgccgattg ccaaagctcc gccccagcaa gcggcattgt ttatgaactg tttgtcgccg
    10921 gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc agaaattgcc tcgctgttag
    10981 cgaaacgttc ctcttgcggt aaagacatgc tctatttctc tctgcgctat ttgcccgtcg
    11041 cgcacggcat tctcgagtat taaatcccct ccaggtaagt cgggtacgac ctggcttacc
    11101 tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcatgc taaagaaaga
    11161 agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc tttgagcgga
    11221 gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt gcgtggtgat
    11281 cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta cccttccgaa
    11341 aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa cgttaaatca
    11401 cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt ttttaaataa
    11461 gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc ccggttatcg cttgtcatag
    11521 ccgtacaccg gaagtgttcc ggctagccac caaccacagc aagcagcaat ccagcaaacc
    11581 ctgcgaagcg gagttgacgc gcgcattact ttttaccgta ttgtctaact ttcttgagca
    11641 atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg acagcgtctg
    11701 ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg ccagttcgct
    11761 atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca gctatagcca
    11821 gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg ataacaaaaa
    11881 tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta tctctgtttt
    11941 taaggcgttt tacagcctga caccgttgaa ttatctcgcc aaacagcgac aaaaagtgat
    12001 gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga aatggcgcgc
    12061 cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc gttgctctat ccaactgagc
    12121 taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga attatacggt caacccttgc
    12181 tgagtcaatg gcttttgatc tggttgctga acaagtgaac gaccgcgtct gattttctga
    12241 tttatttcgc tatagcggca aacaaacgca caccgctgcg cgtctgaatc aagaaaaccc
    12301 gtattttcat gtatcaaagt acaatttacc gccctaacgg aaaattgtcc gctcctatga
    12361 gactggtaac tatgaaacca acgtcggtga tcattatgga tactcatcct atcatcagaa
    12421 tgtctattga agttctgttg caaaaaaaca gtgaattgca gattgtcctg aaaacggatg
    12481 attaccgcat aaccatcgat tatctccgaa cccgtcctgt tgatttaatc attatggata
    12541 tagacttgcc cggaacagac ggttttacct tcctgaaaag ggtaatgct
//
LOCUS       DAJTPG010000107        12460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTPG010000107 DAJTPG010000000
VERSION     DAJTPG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            681..764
                     /locus_tag="OO230_003246"
     CDS             681..764
                     /locus_tag="OO230_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5692897.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(818..2170)
                     /gene="gorA"
                     /locus_tag="OO230_003247"
     CDS             complement(818..2170)
                     /gene="gorA"
                     /locus_tag="OO230_003247"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5692898.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2242..3084)
                     /gene="rlmJ"
                     /locus_tag="OO230_003248"
     CDS             complement(2242..3084)
                     /gene="rlmJ"
                     /locus_tag="OO230_003248"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5692899.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3287..5329
                     /gene="prlC"
                     /locus_tag="OO230_003249"
     CDS             3287..5329
                     /gene="prlC"
                     /locus_tag="OO230_003249"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5692900.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5337..6089
                     /gene="rsmJ"
                     /locus_tag="OO230_003250"
     CDS             5337..6089
                     /gene="rsmJ"
                     /locus_tag="OO230_003250"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5692901.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6138..7607)
                     /gene="dtpB"
                     /locus_tag="OO230_003251"
                     /pseudo
     CDS             complement(6138..7607)
                     /gene="dtpB"
                     /locus_tag="OO230_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7925..8359)
                     /gene="uspA"
                     /locus_tag="OO230_003252"
     CDS             complement(7925..8359)
                     /gene="uspA"
                     /locus_tag="OO230_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5692902.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8753..9088
                     /gene="uspB"
                     /locus_tag="OO230_003253"
     CDS             8753..9088
                     /gene="uspB"
                     /locus_tag="OO230_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5692903.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9159..10658)
                     /gene="pitA"
                     /locus_tag="OO230_003254"
     CDS             complement(9159..10658)
                     /gene="pitA"
                     /locus_tag="OO230_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5692904.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10890..12089
                     /locus_tag="OO230_003255"
     CDS             10890..12089
                     /locus_tag="OO230_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5692905.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3058 a         3144 c         3319 g         2939 t
ORIGIN      
        1 tgacttaacg aatgtgtaag tgcagaggaa ggtaataggt gtgaattttg agttggctat
       61 tcatttgaaa ggcggtttga atcaggacgt aaaaaaaccg actttgcgtc ggttttttac
      121 tcttcagcct gagttggtgg ctctggctgg agtgagaatg cccacatcta acggttcaat
      181 actattagcc agtggctaac attgcaagcg ttagcgattt cttcagctgg agaagggaca
      241 gtttgctata catggtggtg aaagaggatt tatatacttt catcgggtcg ctgccgtacc
      301 aagccttgtc cgaatccgga cgtaaaaaaa ccgacttcgc gtcggttttt ttactcttca
      361 gcccagagtt ggtggctctg gctggagtga gaatgccatc atctaacgca tcaatgctaa
      421 taccatactg aaactattgc aaggttatgc tggttattta gcctaattac actgtatgat
      481 tatccagtta gctctgaggc attttcactc tggcaatgcg cataaacgct ttcaaagtcc
      541 tggtcagaag tacgggtggt gccgttaact gatgctctgg ccggagtgag attgcccaca
      601 tctaacaatg aggcatgcgc cgtgacaggc agtggatgag taagcggatg cattctcact
      661 ccatcgcatg gagaaaacgg gtgattgata aagcaatcat cgttcttggg gcgttaattg
      721 cgctgctgga actgatccgc tttctgcttc agcttctgaa ctgatagcgg aaacgtaatt
      781 aagggctaag agcacactac tcttagccct ttaacattta acgcatggtc acgaactctt
      841 ctgccgccgt tgggtgaatg gcgacggtat tgtcgaagtc ttttttggtt gcgcccatct
      901 tcagcgccac cgcgaagccc tgcaacattt cgtccatacc aaagccaatg ccgtgaatac
      961 cgacaatctt ctcttctggc ccaacgcaca ccagcttcat gcggcacggc tggcggtgag
     1021 tggtgacggc ggtatacatc gcggtgaaag aggatttata cactttcacc tgatcgtcgc
     1081 cgtactgctc gcgcgcctgc ggttccgtta aaccaacagt accaatcggc ggatggctga
     1141 agaccacggt cggaatgttg ctgtaatcca gatgctcatc cggcttgtta ttaaacaggc
     1201 gttcagagag acggcgaccc gctgcaactg ccaccggtgt cagttccact gcacccgtgt
     1261 tatcgcccac cgcgtaaata ccttcaatat tggtgttttg atatttatcg acgacgatat
     1321 agcctttttc gttggtttta acgccagcgg cttcgaggtt gatgttgtca ttggcaggct
     1381 cgcgaccaat cgcccaaatc aggcaatcca ccgtttcact gcgaccatct tccagctcca
     1441 gcgtcaggct accatcggca tttttcacta ccgctttcgg gatggcgttg gtatgcaact
     1501 gtgggccttc ggcgttcatc acttcgacca gcgtttcaga gatcatcggg tcgaagctgc
     1561 gcagcggcgc atgtttacgc acaaacagat gcgttttcgc gccgaggccg ttaatcacgc
     1621 cagccagctc aacggcgatg taacccgcgc caacaaccgc cacgcgctct ggcaaagcag
     1681 gaagggcgaa gaagccatcg gaatcaatac cgtattccac gcctggaata tccgggtggc
     1741 tcggacgacc gccggtggcg atcagaatat gatcggccgt gatggtttcg ccgttaactt
     1801 ccagcgtttt ggcatcaacg aagcgggcaa agcctttgat tacatcaacg ttatttttac
     1861 cgagcacgtt ttcataggaa gtatgaatac ggtcgatata ggcggtacgg ctggcgatca
     1921 acgtttccca gttgaacttg ttgatggtgg tatcgaaacc ataatcaggg ccgtacatat
     1981 ggatcgcttc acggatttgc gccgcgtgcc acatcacttt tttcggcacg cagccgacgt
     2041 tcacgcaggt gccgcccagc tctttggctt caatcagtgc gcatttctgg ccgtacatag
     2101 ccgcgcggtt gatggaggcg ataccgccgc tgccgccgcc gatggcgatg taatcatagt
     2161 gtttagtcat gacatattgt ccttatcgtt gattaccgcg attgtagcgc gagacgtaat
     2221 aggtgccagc aatggctgca attactccgg cacgatccag cttacggtgg cgtgcccggt
     2281 acctgccgga accagtttgc tgtgcagcca cgacagcacg ttattcatct gctgttccag
     2341 cttccacggc gggttaatca caatcatgcc ggaagcggtc atgccacggc gatcgctgtc
     2401 tggcagcacc gccagttcaa tttgcagaat tttgcgaatg ccggtcgctt ccaggtcgtg
     2461 gatcatgcgc ttaatttgct ggcgcagcac caccggatac cacaatgcgt aagtgccagt
     2521 ggcgaaacgt ttgtaacctt ctgctatccc gctgaccacc gcctgatagt cggttttcat
     2581 ttcatacggc gggtcgataa ggattaaacc acggcgagaa accggcggca gtttggcctt
     2641 aagttgctgg aaaccgtcgg ctttttcgac gcgtgcacgg ctgtctttct gaaatccaga
     2701 acgcagcaac gggtaatcgc tcgggtgcag ttcggtcagt tgcaggctgt cttgttcgcg
     2761 cagtagctgg cgagcaatca acggcgaacc ggggtagtaa cgcaactggc cgctacggtt
     2821 gaagtgtttt accacattga tgtacgcctc cagttctgcg ggcaaatcgt cctgctgcca
     2881 gatacgggcg ataccttcga gatattcgcc ggtacgctcg gcatgttcgc tacctaactg
     2941 ataacgcccg gcccctgcgt gggtgtcgag atagagaaac ggtttatctt tctctttcag
     3001 cgactcgatg atcaggctct gaacggtatg tttaaggacg tcggcgtggt tgccagcgtg
     3061 aaagctgtgg cgataactga gcatgggtaa aggtgttccg gtaagtaaaa attggcccgt
     3121 attcggggcg cacaaaagcc tatcaggaca gtataccgaa aagaggccgc cgccgcgaaa
     3181 gcgtaacgtt tctcattgaa attcactaca cttaacccca tgctacacac attatgtaaa
     3241 gcgcctgttg agcgcttcct taacctcttt aaccaggact gcgctaatga cgaatccgtt
     3301 actgactccc tttgaattgc ctccgttttc taaaattctc ccggaacatg tcgttccagc
     3361 cgtgactaag gcattgaacg actgccgcga aaatgtggag cgcgtagtag cgcaaggggc
     3421 accgtacacc tgggaaaatc tctgccagcc gttggcggaa gtggacgatg ttttggggcg
     3481 tatcttctcc ccggtcagcc acctgaactc ggtgaaaaat agcccggaac tgcgtgaagc
     3541 gtacgaacaa accctgccgc tgctgtcgga atacagcacc tgggtagggc aacatgaagg
     3601 gctgtataaa gcgtaccgcg acctgcgcga tggcgatcat tacgccacgc tgaacacggc
     3661 gcagaagaaa gcggttgata acgccctgcg tgattttgaa ctctctggca taggtctgcc
     3721 gaaagagaaa cagcagcgtt acggcgaaat tgctacccgt ctttctgaac tgggcaacca
     3781 gtacagcaac aacgtcctcg atgcgacgat gggctggacc aaactcgtta ccgacgaagc
     3841 ggggctggcg gggatgccag aaagcgcgct ggctgcggca aaagcccagg ccgaagcgaa
     3901 agagctggaa ggctacctgc tgacgctgga tatcccaagc tacctgccgg taatgaccta
     3961 ctgcgacaac caggctctgc gcgaagagat gtatcgcgct tacagcaccc gcgcctccga
     4021 tcaaggcccg aatgccggta agtgggataa cagcaaggtg atggaagaga tcctcgcgct
     4081 gcgtcacgaa ctggcgcaac tgctgggctt tgaaaactac gcctttaaat cccttgccac
     4141 taaaatggca gaaaacccgc agcaggtgct ggatttctta accgatctgg caaaacgcgc
     4201 gcgtccgcaa ggcgaaaaag agctggagca actgcgtgcc ttcgctaaag ccgaatttgg
     4261 tgtcgatgag ttgcagccgt gggatatcgc ttactacagc gaaaaacaga aacagcacct
     4321 ctacagcatc agcgacgagc agctgcgtcc gtacttcccg gaaaacaaag cggttaacgg
     4381 cctgtttgaa gtggtgaaac gtatttacgg catcaccgct aaagagcgta aagatgttga
     4441 tgtctggcat ccggatgtac gtttcttcga actgtatgac gaaaataacg aactgcgcgg
     4501 cagcttctac ctcgatctgt atgcccgtga aaacaagcgc ggcggggcgt ggatggatgg
     4561 ctgcgtaggc cagatgcgta aagctgatgg ttctctgcaa aaaccggtcg cgtatttgac
     4621 ttgtaacttc aaccgcccgg taaatggtaa accggcgctg ttcactcacg acgaagtgat
     4681 caccctgttc cacgagttcg gtcacggcct gcaccatatg ctgacccgca tcgaaaccgc
     4741 tggtgtttcc ggtatcagcg gtgtgccgtg ggatgcggtc gaactgccga gtcagtttat
     4801 ggaaaactgg tgctgggagc cggaggcgct ggcgtttatc tctggtcact atgaaaccgg
     4861 cgaaccgctg ccgaaagagt tgctggataa aatgctggcg gcgaagaact accaggcggc
     4921 gctgtttatt ctgcgtcagc tggagttcgg cctgtttgat ttccgccttc atgccgagtt
     4981 ccgcccggat cagggggcaa aaatcctcga aactctggca gaaatcaaga aactggttgc
     5041 cgtggtgcca tctccgtcct ggggccgttt cccgcacgct ttcagccata ttttcgccgg
     5101 tggttatgcg gcaggttact acagctacct gtgggctgac gtactggcgg cagatgcttt
     5161 ctcgcgcttt gaggaagagg gcattttcaa ccgtgaaacc gggcagtcgt tcctcgacaa
     5221 cattctgagc cgtggcggtt cagaagagcc gatggatctg ttcaaacgct tccgtggtcg
     5281 tgaaccgcag ctggatgcga tgctggagca ttacggcatt aagggctgat cattcagtga
     5341 aaatctgctt aattgatgaa acaggcgccg gagacggcgc cttatctgtt ctggcggccc
     5401 gctgggggct ggagcacgat gaagacaacc tgatggcgct ggtgttaacg ccggaacatc
     5461 tggaattgcg caagcgtgat gagccaaaac ttggcggcat ctttgttgat tttgttggcg
     5521 gagcgatggc gcaccgacgc aaattcggcg gtggtcgcgg tgaggcggtg gcgaaagcgg
     5581 tgggcattaa aggcgattat ttgccggatg tggtggacgc caccgcaggg ctggggcgcg
     5641 atgcctttgt actggcttca gtcggctgcc gcgtgcggat gctggagcgt aatccagtgg
     5701 ttgccgcgct actcgacgac ggcctggcgc gtggttatgc ggatgcggaa atcggcggct
     5761 ggttgcagga gcggttgcag ttaattcacg cctccagcct gacggcgcta accgatatta
     5821 ccccacgccc gcaggtggtc tatctcgatc cgatgttccc gcataagcag aaaagcgcgc
     5881 tggtgaaaaa agagatgcgt gtctttcagt cgctggtggg gccggatctt gatgccgatg
     5941 gattactgga gcctgcacgc ttgctggcga ccaaacgcgt ggtggtaaag cgcccggact
     6001 acgcgccacc gctggcgaat gtcgccacgc caaacgcggt tgtgactaaa gggcatcgct
     6061 ttgatatcta tgcaggtacg ccagtgtaaa agtaaagcag ggtagcgata acgctaccct
     6121 gctttctgca agaataatta atggctttcc ggcgtcgcaa tcatgcgttt cagccacggc
     6181 accatcaaca gcatcactac tgcaacgccc agcgtgacca gaccaatctt accaaacacg
     6241 ttggtataga cgggcaacgt ctcaagcgga tcggtaatgt tgtccggcac cgcggtaaat
     6301 gttgccacat agccgcccag caagaacgcg gcagcctgcg tcaggaacca catcccgaga
     6361 ataaagccca tcaaatgctg cggcaccagg gcagcaatca tcgccaggcc aagggcgcta
     6421 ataaacaatt cacccaggct ctagaataaa tacaccagca cgataaacca tggcgatgtc
     6481 agcccctggt catccgcaaa ccacattccc gcagcagcag ccgtcaaaaa gcccagtgag
     6541 cacataaaca tgctgagagt aaatttcatc ggcatcgaga ggtctttgcc tttgttaccc
     6601 agatgcgtgt aaatgcctgc cagtattggg ctggcgagta ccacccagaa cgggttaagc
     6661 gcctggaagc tgaccgggtt gatggaaaaa ccgagaattt catgatgcac gttgttgatg
     6721 gcaaagaagt tcagcgatgt tggcatctgg gcgtagagaa tgtaaaacac caccgcttcg
     6781 agcatcagga caaaggcgac aaacatttta ttgcgcccgg ttttatccag cttgaatgcc
     6841 tgacgaaaga agatgatagt gacgacgatg gagagaacaa tcagcaccag attggcgact
     6901 tctacgttgt gcatcagcca tgcacatacg aagatcatca ccacgctgcc aagtaatacg
     6961 tacaacagtt tgctgaagct catcggcttg aagtcgggtt cagaaccaat gtctttcacc
     7021 attccacgac aggcgatgta aaccagtaat gcgataatta accccgcccc gcacaggttg
     7081 taggtgactg aataaccgaa tctatcagcg atcacagggg ccagcgataa cgctatcaac
     7141 gagccgatat tgatcgacat atagaacagg gtgaatgcgc catcaagccg cggatctttc
     7201 ggcggatagc acttcgaaag caagctggct gggttagctt taaacaggcc gttaccgaca
     7261 gcgatagtcc ccagggcgat gaaaatcagg tcaggcttaa gtagcgacat gccggtcatg
     7321 aagtagccaa tcgccagcac aagtgctcca agaacaatgg tgcgtttggt ccccaacagg
     7381 tggtcgccga catagccgcc aatggaaatg aggccataga ccagtgcagc aaaagcacca
     7441 aaagtgacaa aagcctgctc ttgcgagaat ccaagctgtt taacgaagaa aaccgccagt
     7501 acgccctgca cgccgtagta gccgaatcgc tcccataact cgacaaaaaa gatcatgaaa
     7561 aatgggcgag gttgctgcag catccccatg ggtgttgttg tattaatatt tattaacctt
     7621 ccaataccat cctgaaaagt atgaacgcgt aagggataac gcgcgaaaaa gtttacatct
     7681 gtcagagatt cttaatattg atgcggtgaa tagattaata cactattaca acagaaaata
     7741 accagctgat cacactaatg atggaaaata tgatggtgtt aaattttgta ttggcaggag
     7801 attttgattg cgtacgcaga tcgtaggcca gataaggggt ttacgctgca tcaggtaaca
     7861 aggcgcgcca aaaagcaaaa cgcccgcata tgcgggcgtt caggaacacg tcgtggaggg
     7921 atgattattc ttcttcgtcg cgtagcggaa caatcagcat atcaacgtga acggtgttga
     7981 tcagctgacg tgcggaagac atcagtttgc tccagaagtc ctggtggtga ccacaaacca
     8041 ccagatccat atcgtatttc ttgattgcat cgaccagaac ctggcctagg tcgccgctgc
     8101 cgctcagggt ttcagtgatt ggatagcctg cattagtgga aagctcggtc agcgcatgat
     8161 gtgtctcttc agagatgcgt ttctgcatat cacccagatt cacatcaata agcccggtgt
     8221 ataggtcaga gtagtttaca tctacgtgga tcagagaaac tttcgcattg taggggcgag
     8281 ccatagagac tgctttctct accagaactt tgctttccgg ggagagatcg actgcgatga
     8341 gaatgtgttt ataagccata gtgttactcc ttccataaag ttgtcgatga ctggccagct
     8401 agcgtttctt gtgccgttac gtcacccgcg tcctgcgaac tacgtgcgcg gggctaaccc
     8461 agccgtaaag attcagtgtt aagaccatcc ttaccttata gcgacccgga tgatccgtca
     8521 atccgccttg cttacccgtt agataaacaa attttcgaca tgaatattat attgatagtg
     8581 gttaaccttc tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat
     8641 gttggtcacc acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg
     8701 ggagcggtag ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag
     8761 caccgtcgca ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt
     8821 ctcatcacta cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata
     8881 tgttgatgga gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt
     8941 ttggtatatc tatgcccaac gttatcgcga tcatcacgat gatgagttta ttcgccgctg
     9001 tgagcgggtg cgtcggcagt ttattctgac tagcgcattg tgtggtctgg tggtggtcag
     9061 cctgattgca ttgatgattt ggcattaagc acttcagcaa taataacgta taaaaaaagc
     9121 gggccagtaa actgacccgc tcgttttggt gcgtacgatt acaggaactg caaggagaac
     9181 cagtacagcc cgccggaaag tagtaccgca gccggaaggg taaacaccca ggccatcaga
     9241 atactggtta cggttttacg ctgtaagccg ccgccatcta ccaccatcgt ccccgcgaca
     9301 gaagaggaga gtacgtgagt ggtggaaacc ggcatcccgg tataactcgc caggccgatt
     9361 gacactgccg ccgtcatctg ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg
     9421 attttctcac cgatagtcgt tgccacacga cgccagccaa tcatcgtacc gatacctaac
     9481 gccagcgcga ccgccatgat gatccacacc ggcgcatact cgatggtgct aagcatgtcg
     9541 gacttcagtt tcttcaacag gcgctgatcg tcagcactca caccaggcat cttcaccact
     9601 ttgtcgatag tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc
     9661 gacagcttgt cgtagctttc cacatcggtg gtcaacatac ctttcaggcg gttgagcgcg
     9721 ttaatggtat tcgacggatg gcagtggaac tccgcaggtt gcgttgcgcc agcttccgga
     9781 gccggtacta actgatcagc accggtagcc tgtttgagca gcgcaggatg ctgctcaaag
     9841 taagcttcga cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc
     9901 atgttcacca cgaagcctgc tggcgcgacg ccaatcaata ccaacataac cagaccaatg
     9961 cctttctgac catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc
    10021 gcgatacgcg tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg
    10081 gtcaggtgga tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc
    10141 agaccgccag caaacaccag gccgacaata ggggaaacga tcagagaacc gaaaatactt
    10201 aatactttcg ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt
    10261 aaaccaatcc cgatgatcgc gccaatcagc gtatgagagc tggatgcagg taaaccaaag
    10321 taccaggtac ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg
    10381 ccatgagacg atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag
    10441 gcaacactca gaccacccag caaaacaccc aaaaagttga acaccgccgc cataaccacg
    10501 gcgagctgag aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca
    10561 tggaaaccat tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc
    10621 ccggtatgca aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg
    10681 acatgaacgc ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt
    10741 tttttgattt gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg
    10801 caaatacttc cttttagtaa tattgatgct ggtgcgacca ctgaggaatc tttacaattc
    10861 acgcccgttt tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg
    10921 ctggtgcggc gggtatgttc tgttctgcgc tggcaggtca ggtaggacgc cgggttctgc
    10981 tgatcgataa tggtaaaata ctagggcgca aaatccttat gtctggcggt gggcgctgca
    11041 actttaccaa cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta
    11101 agtctgcact cgcacgtttt acccagtggg atttcattga tctggtcaat aaacacggta
    11161 tcgcctggca cgagaaaacg ttagggcaac tcttctgcga tgactccgcg cagcagattg
    11221 tcgacatgct ggtggatgag tgcgagaagg gcaatgtaac cttcagattg cgtagcgaag
    11281 tgctgagtgt ggcgaaggat gaaacaggct tcacgcttga tctgaacggc atgactgtcg
    11341 gttgcgaaaa gctggtcatc gcgaccggtg ggctgtcaat gccggggctg ggcgcatcgc
    11401 cgtttggtta taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgcgcgggtc
    11461 tggtgccatt cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg
    11521 cggtgccttc cgtgattacc gctgaaaacg gcaccgtttt ccgtgagaac ttactcttca
    11581 cccaccgcgg cttatctgga ccggcggtgt tgcagatttc aagctactgg caaccgggtg
    11641 aatttgtcag tatcaatctg ctaccggatg tggacctcga aaccttcctg aatgagcagc
    11701 gtaacgcaca tccgaatcaa agcctgaaaa acacactggc ggttcatcta ccgaagcggt
    11761 tggttgaacg cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg
    11821 tgtgtgacca acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca
    11881 ctgaaggcta tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactttctt
    11941 cacggacgat ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg
    12001 ttaccggctg gctggggggc tataacttcc agtgggcgtg gtcgagtgca tgggcttgtg
    12061 cgcaggattt ggcgtcaatt gtgaaataac catgacgttt tcccgtcagc cgtttgtata
    12121 tgtcgttatt aagtcatttt ggtacggttc tgcatccgac attctggtgt taactcgctg
    12181 ttttgttagc gaattaacgc tattgccgtt gtggttattt tatagtgtat ttattctcag
    12241 agaatatata ttactgaaat aaaaaacaca aagaataacc aaaggaaatt atttgaaacg
    12301 taaaaggtat atatttttaa tctgtaaaat ttaaaatcac ctcctctgga ttgaatcaac
    12361 ttgctctatt taagacgatt gtttttacat tttcaccacc tgtatcaggt ggtgtttttt
    12421 tattggcggc aatgccttta tcttattttt attacgttgt
//
LOCUS       DAJTPG010000108        12112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTPG010000108 DAJTPG010000000
VERSION     DAJTPG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..369
                     /locus_tag="OO230_003256"
     CDS             <1..369
                     /locus_tag="OO230_003256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692906.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO230_003257"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO230_003257"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5692907.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO230_003258"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO230_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5692908.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO230_003259"
     CDS             complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO230_003259"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5692909.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO230_003260"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO230_003260"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5692910.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO230_003261"
     CDS             complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO230_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5692911.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5094..6755)
                     /gene="tar"
                     /locus_tag="OO230_003262"
     CDS             complement(5094..6755)
                     /gene="tar"
                     /locus_tag="OO230_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5692912.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="OO230_003263"
     CDS             complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="OO230_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5692913.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="OO230_003264"
     CDS             complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="OO230_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5692914.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9393..10319)
                     /gene="motB"
                     /locus_tag="OO230_003265"
     CDS             complement(9393..10319)
                     /gene="motB"
                     /locus_tag="OO230_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5692915.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10316..11203)
                     /gene="motA"
                     /locus_tag="OO230_003266"
     CDS             complement(10316..11203)
                     /gene="motA"
                     /locus_tag="OO230_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5692916.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="OO230_003267"
     CDS             complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="OO230_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5692917.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11911..>12112)
                     /locus_tag="OO230_003268"
     CDS             complement(11911..>12112)
                     /locus_tag="OO230_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5692918.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2783 a         3387 c         2934 g         3008 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgaac cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagag aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca
     4981 atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga
     5041 ttaaacatca acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat
     5101 gtttcccagt ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca
     5161 ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac
     5221 gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct
     5281 gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat
     5341 gcgacttgat cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg
     5401 tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc
     5461 agcaccgaac cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt
     5521 gccgccaggg cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca
     5581 cggccctgtt cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc
     5641 tggaaggcaa taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg
     5701 atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg
     5761 gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc
     5821 gcggtgagct gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta
     5881 cgggaggaaa gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct
     5941 gaaccttcgc ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc
     6001 tgcgccaggt cacccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca
     6061 ccggcgattt cgcaaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga
     6121 atgccgtacc acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc
     6181 cactgggcaa atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca
     6241 ctgctgaggg cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc
     6301 tgagcgaaat aagctccagt attgccataa tcaagataat caatcagttc agttaacgct
     6361 gtgtaatagt ttttatattt ttcatcaaaa ttacggctgg tagcgaccat ttcaggtaac
     6421 ggtgccatgc ttttgaattt tttataatgc gtcgcggcct gggccaatgt tttcctggcg
     6481 ctatcgagca attcaacttt ggcgttactt tgttgattgg aggaatccat catcatccgc
     6541 accgctgaac gactcaggtt aatgcgcgtt tgcagcatta aatcccaggt tgacgtcagc
     6601 tcgccctgct gttcccgtaa ttgattggaa accacaaagc tcttctggct atggtgaagg
     6661 gaagaaaaaa acaaactgcc ggaaataagc tgtaacagtg cgaatacccc cagcaccatt
     6721 accaacagcg tgactacgcg gatacggtta atcataaggc accttcctga aaacaagttg
     6781 atctcgttat cggcaagaag gggggaaact ttattgctga tgccacccgc cgcgaaattg
     6841 aaataaaaaa cccgatgcgc agatcatcgg gttcatttca attgaggaaa tcgggagaat
     6901 tacgccactt ctgacgccgc gctatctaac agcgccatct cttcgctgtt cagcagtttt
     6961 tcgatgttca ccagaatcaa catacggtcg cccagtgcgc ccagtccagt gagatattct
     7021 gttgaaagcg tcacggcaaa ctctggtgcc ggacgaattt gctccgccgt caatgaaagc
     7081 acgtctgaaa cgccgtcaac cacgatgccg accacccgct gtccgagatt caggacgata
     7141 actaccgtgt tgtcgttata gtccacatcc acctggctga acttaattcg taagtcaaca
     7201 atcggcacaa taacgccgcg cagattcgtg acgcctttga taaacgctgg cgtgttcgca
     7261 atccgtgtta cctgatcgta gccacggatc tcctgcactt tcaggatatc aataccgtac
     7321 tcttcatcac caagggtaaa taccagaaat tcctggcctg acggctcgct ggccagcttt
     7381 gttacattcg tcataccggt catattgtta cctttttact cattcaggcg gcggtgttcg
     7441 ccatacgttg ttcgcggttt atcgcctgca aggcggagac atcaacaatc agtgccacgc
     7501 tgccgtcgcc aagaatggtc gcagcagaaa tgccggggac tttgcgatag ttactttcaa
     7561 ggtttttaac cacaacctgg tgttgaccaa ttaattgatc caccagcaag gcgtagcggc
     7621 gaccgccact ttgtaagatc accacaattc cctgggtggc ttcggttttc gcgcccgcga
     7681 cgttgaacac tttccacagt tcgacgatgg gcagatattc accccgcact tccagcaccc
     7741 gctcgccgcc ggccagtgga tggagatcgg cttcacgggg ttgcagtgat tccataacag
     7801 cattcagcgg cagaatgaaa acttcatccg caacgcgtac ggacatgccg tcgaggatgg
     7861 ccagcgtcag cggcagtaaa atgcggatcg tagtgccagt accctgcttc gactggattt
     7921 cgacatgacc gcccatctcc tggatattac gtttaacgac gtccatgccg acgccgcgcc
     7981 cggagacgtc ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc agcattgcaa
     8041 cttcgtcgtc gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa
     8101 ttcgctcacg gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct
     8161 gatgttcggc agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt
     8221 ctggcagttc aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa
     8281 tgcgttctat caggctcttg tcgagctcgg tggaactgcc caccagcgtc agttctacct
     8341 gcttgccgag ttttcccgcc agatcacgca ccagccgggg atagcgacta aagacatatt
     8401 ccatcggcat catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta
     8461 actgccccat gctggtaatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct
     8521 gggcaagcat ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct
     8581 tttctaccgc tacacggatg ctggtggatt cactgctgcg cgtcgttttt tcccgctcca
     8641 cgcggccggt tggcgcttgt tcggctgcca gtttaagcac aggtggggta gctatttttg
     8701 gcgaaacttc tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg
     8761 ctgtgatgtc atcttcggcg atatcgcccg gtaatattgc cgagagagaa tccgcccctt
     8821 tcaccacgtc atttaacgtt gtcagatgcc ccagctcttc ttccagcagg tcgacttcgc
     8881 cggccttcag gcgcgaaagg ataattcgtc gcggcaactg actgcgactt tgctcatctt
     8941 gcggttcact tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg
     9001 cttctaatgc cagttgacgc aaggcctggc agatataatc gtagctggcg gcatccggct
     9061 cttgcgactg tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca
     9121 gattgataat gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt
     9181 ccatcagatg cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga
     9241 tcgagtgggc agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca
     9301 aaaccagcag atgctgctcc atatcagcca acagttcgtc cgcttcatca aaaaatgtct
     9361 gataaaaatc gcttatatcg atgctcacgc tgtcacctcg gttcggctga tggcattgtg
     9421 ggaacactga cctgtggtgc aacctcaggt ttttctaggg cgcttactgg ctcattctgg
     9481 ctttcggcgt tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac caacaggctg
     9541 atgcgacggt tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg
     9601 ccgacgacac gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat
     9661 gcattggccc gatcggcaga aagctcccag ttgctgtaac ctttctcacc gctggcgtag
     9721 ggaaaatcat cggtatgacc tgaaaggcta atacggttgg gaataccgtt cagcacaggc
     9781 gcgatggcgc gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac
     9841 atcgggcgat tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt
     9901 ttgagatgga gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca
     9961 ccccgcaatt tccgcaatcg actttgctcc atgcgttttt tcagctcttc gatgttcggc
    10021 tgcttattca cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt
    10081 tcactattgg aaatgcgatc gccgcccgta accgctgtcg ccagtggagt ccggaagtac
    10141 tccgcaatct gaatcaactc ttttgggctg gagatggaga tcagccacat caccagaaaa
    10201 aaggccatca tcgcagtcat aaagtcggca taagcaatct tccacgatcc atgtgctgcc
    10261 ccgtggcttt tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg
    10321 cttcctcggt tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt
    10381 caataaacga cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag
    10441 gcggtgcgta accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc
    10501 tggtttcggc gcttttctga cgtaaaacac tcgctagtgg ggaaataaat ccgtaagcca
    10561 ataaaatacc gaggaaagtc cccaccatcg catgtgcgat aagcgccccc agctcggcgg
    10621 caggacgatc ggctgaacct aacgcgtgaa cgccccccat tacagccgcg acaataccaa
    10681 acgccggaag tgagtccccg accagcgcca gactgttcgc cgggacttct gcctcgcttt
    10741 cgtgcgtctc aatctcttca tccatcaggg cttcgatttc gaaggtgttc atgtgaccgc
    10801 tgataatcag gcgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc
    10861 gtgggtagct ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa
    10921 acatccccat ctgccgcgat ttcgccatca accgataaag cagagccagc agatccatat
    10981 acattgcttt ggtgtatttg gagcgacgaa acagcaacgg cagcgctttc agcgtgcctt
    11041 taatcgcttt gccattattg ccgacgataa acgacccaat ccctgcaccg gcaataatca
    11101 ccagttcagc gggttgatag agtgctccaa ggcttccacc ggtcatcaaa taaccgccga
    11161 aaactgtacc gagaacaacc aggtaaccta ataagataag cacgacatca tccttccact
    11221 gttgaccatg acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg aaaaaaaaag
    11281 cagcggtacg tcgttaccgc tgctggaatg ttgcgccaca ccgtatcagt taaacagcct
    11341 gtactctctg ttcatccagc agttgtggga taatatcggc aggattctgg gaaagtttac
    11401 gtctttttac tgcccgggat ggcggttgac ataagctgca ggcaaagctg ccaacaggct
    11461 ggtgagcgtg ggtaataaaa ttgccgccgc agcagttgca gctggaaagt tgcagtaatc
    11521 cactttcaac aaaccgcacc aatgtccagg cacgggttaa tgccagcagt ggtccttctt
    11581 ctgcttgtgg gcactgttca aggtataaac ggtaggcttt gatcaccgca tcgacgccat
    11641 tacacaaacc ggttttcagt aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt
    11701 tttgttccca ggtcataaac cagtcggttg agaatggcag catgcctttc ggcggtgggc
    11761 ttccgcgcag ttctttataa agttttatca ggcgtccgcg acttaactgt gtttcgcttt
    11821 ccagcatctg caaacgagcg cccagggtga tcaattccat tgccagctga atatcccgcg
    11881 cttcctgaac aatgcttttt tcactcatga tcaggccctt ttcttgcgca gcgcttcttc
    11941 aggctgatta acatcattca gcaagcgtgt tgagagcatg atgccggtat gaatttgctg
    12001 gagatcgtca acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg
    12061 gaagtgacaa accagctgat tggtttctgc cagcttaacc atttgcggaa ga
//
LOCUS       DAJTPG010000109        12090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTPG010000109 DAJTPG010000000
VERSION     DAJTPG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            345..1985
                     /gene="trg"
                     /locus_tag="OO230_003269"
     CDS             345..1985
                     /gene="trg"
                     /locus_tag="OO230_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5692919.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2023..2946)
                     /locus_tag="OO230_003270"
     CDS             complement(2023..2946)
                     /locus_tag="OO230_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5692920.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3163..4506
                     /locus_tag="OO230_003271"
     CDS             3163..4506
                     /locus_tag="OO230_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5692921.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4731..6386
                     /gene="opgD"
                     /locus_tag="OO230_003272"
                     /gene_synonym="mdoD"
     CDS             4731..6386
                     /gene="opgD"
                     /locus_tag="OO230_003272"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5692922.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6526..6750
                     /locus_tag="OO230_003273"
     CDS             6526..6750
                     /locus_tag="OO230_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5692923.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6813..7349
                     /gene="rimL"
                     /locus_tag="OO230_003274"
     CDS             6813..7349
                     /gene="rimL"
                     /locus_tag="OO230_003274"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5692924.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7344..8324)
                     /locus_tag="OO230_003275"
     CDS             complement(7344..8324)
                     /locus_tag="OO230_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692925.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8448..9440
                     /gene="tehA"
                     /locus_tag="OO230_003276"
     CDS             8448..9440
                     /gene="tehA"
                     /locus_tag="OO230_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5692926.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9437..10030
                     /gene="tehB"
                     /locus_tag="OO230_003277"
     CDS             9437..10030
                     /gene="tehB"
                     /locus_tag="OO230_003277"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5692927.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10333..10989
                     /locus_tag="OO230_003278"
     CDS             10333..10989
                     /locus_tag="OO230_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5692928.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11007..11513)
                     /gene="tnpA"
                     /locus_tag="OO230_003279"
                     /pseudo
     CDS             complement(11007..11513)
                     /gene="tnpA"
                     /locus_tag="OO230_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11521..12090
                     /locus_tag="OO230_003280"
                     /pseudo
     CDS             11521..12090
                     /locus_tag="OO230_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3130 a         2827 c         3039 g         3094 t
ORIGIN      
        1 aaatgggtgc agtacagttg atagaaatat cggtcggggc cttcgtcttt ctgattcccg
       61 gttagcctga aaacagaaag tctcaggcac ccgcaggcat cctatgaggt ttccttaggg
      121 acgaaaataa tcacttcacg aaattgcgtg ctgttttcca gaatttttcg tcattcgggt
      181 tagccagttt agccattcgt tactctcttc attccaatag cattaatttt ctctgcaata
      241 attgttgtaa aaatgtgacg caaagaggtt tttggtcata agtaattacc gtcaagtgcc
      301 gatgactttc tatcaggagt aaacctggac gagagacaac ggtaatgaat acaactccct
      361 cacagcgatt aggttttttg catcacatca ggctggttcc gttatttgcc tgcattcttg
      421 gcggtatctt agttctattc gcattaagtt ctgctctggc tggctatttc ctctggcagg
      481 ccgatcgcga tcagcgtgat gttactgcgg agattgagat tcggaccggg ttagcgaaca
      541 gttcagattt tttgcgttca gcccggatca atatgattca ggccggggct gcgagtcgta
      601 ttgcggaaat ggaagcaatg aagcgaaata ttgcgcaagc cgaatcggag attaaacagt
      661 cgcagcaagg ttatcgtgct tatcagaatc gaccggtgaa aacacctgct gatgaagcct
      721 tcgacactga attaaatcaa cgctttcagg cttatatcac gggtatgcaa cctatgttga
      781 aatatgccaa aaatggcatg tttgaagcga ttatcaatca tgaaagtgag cagatccgac
      841 cgctggataa tgcttatacc gatattttga acaaagccgt taagatacgt agcaccagag
      901 ccaaccaact ggcggaactg gcccatcagc gcacccgcct gggtgggatg ttcatgattg
      961 gcgcgtttgt gcttgccctg gtcatgacgc tgataacatt tatggtgcta cgtcggatcg
     1021 tcattcgtcc actgcaacat gccgcacaac ggattgaaaa aatcgccagt ggcgatctga
     1081 cgatgaatga tgaaccggcg ggtcgtaatg aaatcggtcg cttaagtcgt catttacagc
     1141 aaatgcagca ttcactgggg atgacagtag ggactgttcg acagggcgcg gaagagattt
     1201 atcgtggcac cagcgaaatt tcagctggca atgcggacct gtcatctcgc accgaagaac
     1261 aagcggcggc tatcgaacaa actgccgcca gcatggagca actcactgcg acggtgaaac
     1321 agaatgcgga taacgcgcat catgccagca aactggcgca agaggcttct attaaagcca
     1381 gcgatggcgg gcagacggtt tccggtgtag taaaaacgat gggcgctatc tccacgagtt
     1441 cgaagaaaat ttctgagatc accgccgtca tcaacagtat tgctttccag acgaatattc
     1501 tggcactgaa tgctgccgtt gaagccgcgc gagcgggtga gcaagggcgt ggatttgccg
     1561 ttgtcgccag cgaagtacgg acactcgcaa gccgcagcgc tcaggcagcg aaagagattg
     1621 aaggcttgat cagtgaatca gtcaggttaa ttgacctggg gtcggatgag gtggcaacgg
     1681 cagggaaaac catgagcact attgttgatg ccgtcgcgag tgtcacacat atcatgcagg
     1741 aaatcgccgc cgcctcggat gaacaaagta gaggcataac tcaggttagc caggcgattt
     1801 ctgaaatgga taaggtgacg caacagaatg cttctctggt agaagaggcc tcagcagcgg
     1861 cggtgtccct tgaagaacag gctgcacgat taactgaggc ggtgaacgta ttccgtctga
     1921 acaaacattc tgtgtcggca gaacctcgcg gagcgggtga accagttagt ttcgctacgg
     1981 tgtgaaaatg ttcaaggagg gatcgacaga tcccttcacc tttcagaacg gcattgattt
     2041 tcgaatagcg ttaatcatca actggcaacc agaagagaac gtcgcatcta cgcgggtcag
     2101 tattccaatc ggttcgcctg caccatgtcc cggaacaggc agggccacca gcgtggcatg
     2161 acgcaggtcg tcttttacag cgccagaagg gacaaaccac acgtaatcgt attcaacggt
     2221 aagttgacga gatagcgaag tagacagcgt ttcgatacaa cccgaaggaa ttttacagcc
     2281 ctggctctgc actaatgcat ctgaatgctg gcgtggcgca gtgccttctg gtgatacaac
     2341 gaccggccat tccagcaccc ggcttagcgt tacgttctcc tgaagtagcg ggtgattagg
     2401 gcggacaacc agcttcaacg attcaagaaa cagcagttcg taattaagcc cggtcatcag
     2461 ttcaggatct gacatccgac caatgccgat atcgatttcc ccggttttta aacccgccag
     2521 aatcataggg ttactcattg tcgcaacttg caaggtcgtc tctttttgtt gctgatgaaa
     2581 ctgacctata accgaaggta atatccccag tgccgcagta ggtagtgcac caaccctgac
     2641 gacatcatta ttaagacctt ctttacgatg aagcgactgt ccggcagtgt tgatggcgtc
     2701 aagaactctg actgcatgcg ttaaaaattg ttcgccgggt aaggtaagtt gcgccccctg
     2761 acgaccacgc tcaaacaagc gagcgcccgt cagctgctcc agttcattca atgtattaga
     2821 gagcgcaggt tgactcaaat taagggtttc agctgcgcgc cccaaagttc cttgttgtgc
     2881 gacagctacg aatgtatgaa ggtggcgcaa acggatgcgc tgactaaaca gactattttt
     2941 ttccataagc gatgttaaaa acgaagcggt gtcgctgaca agtgaagttg tttgattatg
     3001 ataacttgat tgcaaaatat tattaacaat taaagcaatt atgttacagc aaaatggata
     3061 atattgatgt tttcgcggcg agatcacagt ttgtaaattc ttcccgccag agtgaatgcg
     3121 gttacctaca ctccagatta ctgaccactg gaggcagaca ctatggcgaa cagcatcacg
     3181 gcggatgaga ttcgggaaca gttttcgcag gcaatgtcag ccatgtacca gcaagaagtt
     3241 ccgcaatatg gcacgctgct ggaactggta gctgatgtga atctggctgt gctggaaaac
     3301 aatcctcaac tgcacgaaaa aatggtaaat gcagacgagc tggcgcgact gaatgttgaa
     3361 cgtcatgggg cgattcgcgt tgggactgca caagagcttg ctactcttcg gcggatgttt
     3421 gccattatgg ggatgtaccc ggtgagctat tacgatctct cgcaggcagg ggtgccggta
     3481 cattcgacag catttcggcc cattgatgat gcttctctgg cgcgtaatcc cttccgcgtt
     3541 tttacatcct tactccgcct tgagcttatc gagaacgaaa ttttgcgcca gaaagcggcg
     3601 gagattctgc gtcagcgcga tatcttcacc ccacgttgtc gacaactgtt agaggaatat
     3661 gagcagcagg gcggttttaa cgaaacacag gcacaggagt ttgtgcagga agccctggaa
     3721 acgtttcgct ggcaccagtt agcaacggta gatgaagaaa cctatcgcgc cttgcacaac
     3781 gaacatcggt tgattgctga tgtggtctgt tttcctggat gccatatcaa ccacctgacg
     3841 ccacgtacgc tggatattga ccgggtgcag tcgatgatgc ctgaatgcgg aattgaaccc
     3901 aaaattctca tcgaggggcc gccgcgccgc gaggtaccga ttttactacg ccagaccagc
     3961 tttaaagcac tggaagagac ggtgttgttt gcggggcaga aacagggcac gcataccgcg
     4021 cgctttggtg aaattgagca gcgtggcgtg gcattaacgc cgaaagggcg acaactgtat
     4081 gatgatcttc tgcgtaacgc tggaaccggg caggataatc tcactcacca aatgcattta
     4141 caggaaacct tccgcacttt tcctgacagt gagtttttaa tgcgtcagca agggttggca
     4201 tggttccggt accgtctgac gccttcgggt gaggcgcatc gtcaggcgat tcatcctgga
     4261 gacgatccac agcccttaat tgaacgtggt tgggtagtgg cgcaacccat cacctatgaa
     4321 gatttcttgc ccgttagcgc ggcggggatc ttccagtcaa atctgggtaa tgaaacgcag
     4381 acacgcagtc acggtaatgc cagtcgcgaa gcatttgagc aggcgttggg ttgtccggtt
     4441 ttggatgagt tccagcttta ccaggaagcg gaagaacgca gtaaacgtcg ctgtggtttg
     4501 ctttaaattc tgaccatccg cctttgcaaa aatttgcctg attttacaaa cgaatcaggc
     4561 tcatcccatc gacataaaaa aaatgccgat ttatgcatat tctctcagtt caacaattgg
     4621 attattaata aatattgtct agagtgagcg gtcataaata agcactttct tgccgctgaa
     4681 aacgaccagc gcgggaccat tcacaacacc agaaggactc actttcaggt atggatcgta
     4741 gacgatttat taaaggttca atggctatgg ccgccgtgtg cggtaccagc ggcattgctt
     4801 ctcttttttc tcaggcagca ttcgcggcag attctgatat tgccgacggg caaacccagc
     4861 gttttgactt ctccattcta cagtcaatgg cgcacgactt agcgcaaaca gcgtggcgtg
     4921 gtgcgcctcg tccgttacct gacacgctgg cgacaatgac gccgcaggct tataacagta
     4981 ttcaatacga cgccgaaaaa tcgctctggc ataacgttga gaaccgtcaa ctggacgctc
     5041 agttcttcca tatgggaatg ggattccgtc gccgcgttcg tatgttttct gtagatccag
     5101 caacacatct ggcgcgtgaa attcactttc gcccggagtt gttcaaatac aacgatgcag
     5161 gtgttgatac caaacaatta gaagggcaaa gcgatctcgg ctttgccggt tttcgcgtgt
     5221 ttaaagcccc cgaactggcg cgccgtgatg tagtatcatt tctcggcgcg agttatttcc
     5281 gcgccgttga tgatacatat caatacggtt tgtcggcccg cggcctggcg atcgacactt
     5341 acaccgacag taaagaagag ttccccgact ttaccgcctt ctggtttgat acggtaaaac
     5401 cgggggcaac tacctttacc gtttatgcgt tgctcgatag cgccagcatt actggtgcct
     5461 ataagttcac tatccattgt gagaaaagtc aggtgattat ggatgtggaa aatcacctgt
     5521 atgcgcgcaa agacattaaa cagctgggca tttcgccgat gaccagtatg ttcagctgcg
     5581 gtactaatga acgtcggatg tgcgatacaa ttcatccgca aattcatgac tctgatcgtc
     5641 tgtccatgtg gcggggcaac ggcgagtgga tttgccgtcc gctgaataat ccgcaaaaat
     5701 tgcagttcaa tgcttacacc gacaacaacc cgaaagggtt tggtttattg caactggatc
     5761 gtgacttctc ccattatcag gacattatgg gctggtataa caaacgccca agtctgtggg
     5821 tggaaccgcg taacaagtgg ggtaagggca ccatcggcct gatggaaatc ccaacaacgg
     5881 gcgaaacgct ggataacatt gtctgcttct ggcagccaga aaaagctgta aaagcaggtg
     5941 atgagtttgc attccagtat cgtctgtact ggagtgcgca accgcctgtt cattgcccat
     6001 tagcgcgcgt tatggcgacg cgtaccggca tgggcggttt cccggaaggt tgggcgccag
     6061 gtgaacacta tcccgaaaaa tgggcgcgtc gttttgccgt cgatttcgtt ggtggtgatc
     6121 tgaaagctgc cgcgccaaaa ggcattgagc cggtgattac gctttccagt ggggaagcga
     6181 agcaaatcga aattctctat attgaaccca tcgatggtta tcgtattcag tttgactggt
     6241 atccgacttc ggactccact gatccggtcg atatgcggat gtatctacgt tgtcaggggg
     6301 acgctatcag tgaaacatgg ctgtatcagt atttcccgcc agcgccggat aaacgtcagt
     6361 atgttgacga ccgcgtgatg agttaatcgt tttttcttcg gcaccttctt cgggaggtgc
     6421 cgtctggtta aacacgatcc cgctcgcatt tttccctaag ttaaatgagt aatctgatgg
     6481 tgtgtatttc agatacaccg tgtcagccac taacagggag tgcgtatgtt tccagaatac
     6541 cgagatttaa tatcccgtct gaaaaacgaa aatcctcgct ttatgtcctt gttcgataaa
     6601 cacaataaac ttgatcatga aattgccaga aaggaaggtt ccgacggtcg agggtacaat
     6661 gcggaagtgg tccgcatgaa aaaacaaaag ctacagttaa aagatgagat gctcaaaatc
     6721 ctgcagcagg agagcgtcaa agaggtgtaa actttcctaa gccgccagcc aggcggcttt
     6781 tttaacaaca gcatggattg actggagata agatgactga aacgataaaa gtaagcgaat
     6841 cacttgaatt acatgctgtt gcggaaagtc acgtcacacc tctttatcag ttaatctgta
     6901 aaaataaaac ctggttacag cagtcgctaa actggccgca gttcgttcag agtgaagagg
     6961 acacgcgaaa aacggtgcag ggtaatgtga tgttgcatca acgcggctat gccaaaatgt
     7021 tcatgatttt caaagaagat gaacttatcg gcgttatctc gtttaatcgt attgaaccac
     7081 tgaataaaac tgctgaaatc ggctactggc tggacgaatc tcatcagggg caggggatca
     7141 tttctcaggc gctgcagaca ttgattcatc attacgctca gtctggtgaa cttagacgct
     7201 tcgtgatcaa atgtcgggtg gacaatccgc aaagcaacca ggtcgctttg cgcaatggtt
     7261 ttatccttga aggttgcctg aaacaggctg agttcctgaa tgatgcctat gatgatgtga
     7321 acttatacgc gcgtattatc gattcataat aaccctgcca gcggcgttcg cgtaatgcgc
     7381 tcctcgccgt taatgacttt tggaccacgg acatgaaccg tgtcgccatc aaaggcttca
     7441 acgacggcgt ggtcggttaa ctccacatgg ttttcaatta tcaccgcacc tgtaatacgg
     7501 ctctcacctt gtattacaac gtgctcgtcg agcagaatcg gtccaccacg taccacggca
     7561 tttccgccaa tcaagacatg atgttttaac acacaattac cttccacaat ggcatattcc
     7621 gccacctgcg aactgtaatg aatcgtcgga atggcatctt cttctatgcc agctttcacc
     7681 tgcgcgtgac cgtagacttt agcgcaatcg catagccaga cgttgttctc ttcattacct
     7741 tcaacactgg caaaatcaaa aacttcggcg cgatgttcaa taaaagcata ccggacgacg
     7801 gcatcgccat aaatttgtgc ctggtggaca atacgcgagg cgctaaccct tgcgcgatca
     7861 taaatttgga gcaggagttg atggtcgggc gttaagcctt gtgcggcgac gatcatagaa
     7921 tgttgatcaa ttaaggcgtg accaaatatt ctacattgca cgtaaactaa tgagtcatga
     7981 atggtgacac tgtcacttat atatgcaccc tggctgattt cactattatc gatccagacg
     8041 ttatccgttg cgtaaacctc tccccacagc acgctggtgc cggtgatacg cgtattaccg
     8101 gaaatcaccg ttccgccgaa cgcaatagca ttctggtcgt aaatccagca atttccttcc
     8161 tgtgctaaca ccgtttcgcg atcgatccag ccgccagcgg ttcctgctat cacatcgtta
     8221 aaatcgctaa tggcgataat ctgccgtaat aacacacttt ttttagtgcc atcttcctga
     8281 taactaaagg ctcgctgttc ttcacttagg cgatatttgc gcatagcgtt ttcccacagg
     8341 tgacttacta taaccgtagc aaattctgcg gctctggcta tgctcgagaa aattccataa
     8401 aatgcatttc aaatatactt tataaattaa acaaaatgag taagaagatg cagagcgata
     8461 aagtgctcaa tttgccggca ggctactttg gtattgtgtt ggggacgata gggatgggat
     8521 ttgcctggcg ctatgccagc caggtttggc aggtcagcca ctggttaggg gatgggctgg
     8581 tgattctggc gatgatcatc tggggattat tgactagcgc atttattacc cgactcatac
     8641 gctttccgca tagcgtgctg gcggaagttc gccatccagt gctgagcagt tttgtgagtt
     8701 tgtttccggc aacgacgatg ctggtggcga ttggttttgt tccgtggttt cgcccactgg
     8761 cggtgtgcat gttcagtttt ggtgtcgtgg ttcagttggc ttatgccgcc tggcaaactg
     8821 cgggattatg gcgcggatct caccctgaag aagctaccac gcctggactg tatctgccga
     8881 cagttgccaa caactttatc agcgcaatgg cctgtggtgc gttgggctac accgacgccg
     8941 gtctggtgtt tttaggcgca ggcgttttct catggctaag ccttgaaccg gtgatcttgc
     9001 agcgtctgcg cagttcggga gaattaccca cggcactgcg gacatcactc ggcattcagc
     9061 tcgctcctgc gctggtggcc tgtagtgcct ggctgagcgt caacggcggc gagggtgaca
     9121 cgctggcgaa aatgcttttc ggttatggac tgctgcaact gctgtttatg ctacgtctga
     9181 tgccatggta tctctcccag ccatttaatg cttcattctg gagtttctcg ttcggcgtat
     9241 ctgcactggc aaccaccggt ttgcatctgg ggagtggcag cgataatgga tttttccata
     9301 cgctggcggt gccgctgttt atctttacca attttattat tgcaatactg ctcatccgta
     9361 cttttgcgct tctgatgcag ggaaaattgt tagtcagaac cgagcgcgcc gttttaatga
     9421 aagcagagga caaagaatga tcattcgtga cgaaaactat tttactgata aatatgaatt
     9481 aacccgcaca cactctgaag tactggaagc ggtgaaagtg gttaaaccgg gtaaaacgct
     9541 ggatctgggc tgtggcaatg gtcgtaacag tctttacctg gcagccaatg gttatgatgt
     9601 tgacgcatgg gataaaaatg ccatgagtat cgccaacgtc gagcgcatta aatccattga
     9661 aaatctggat aatttacaca cccgggtcgt tgatctgaat aacctcacat ttgatggaca
     9721 gtacgatttt attctttcga ctgtggtgct gatgttcctt gaggctaaaa ccatcccagg
     9781 gttgattgcc aatatgcaac gttgcactaa acctggtggt tacaacctga ttgtggcggc
     9841 gatggatacc gctgattatc catgtaccgt cggcttcacg tttgccttca aagagggaga
     9901 attacgtcga tattacgaag gctgggagag ggtgaaatac aatgaagacg tcggcgagct
     9961 gcaccgcacc gacgccaacg gtaatcgtat taaactgcgt ttcgccacga tgctggcacg
    10021 taaaaaatga cccggtaagc acaaaacgcg tgaaaattcc ccacgctgag atgatttact
    10081 gttcttcttt tcggtaagca tattttttat cgaagggatg tgaaattaat cacagtagtc
    10141 gaagtttttt agcagcttaa cttactgaaa tttaactact gatgattgac ttagcccctt
    10201 tttcggcatt gactatgtcg tctgaaaagg ggctgaaaaa tttattttca ccaacacttt
    10261 ttttgccaca acacgaagcg gcgctttttg ctataactta gaaagtaata taatcatctc
    10321 aggaaactat tcatgcgtac cacatcattt gcgaaagttg cagctttatg cggcttgttg
    10381 gctctgtctg gttgtgcctc taaaatcacc cagccagata aatataacaa ttactctgat
    10441 ttaaaagaaa caacctcagc aacaggtaaa cctgttttac gttgggtaga cccgagtttt
    10501 gatcaaagca aatatgacag catcgtctgg aacccaatca cttattatcc ggtaccaaaa
    10561 ccttctaccc aggtagggca gaaagttctg gataaaattt tgaactatac caacaccgaa
    10621 atgaaagaag cgatagcgca gcgtaaacca gtggttacca ccgctgggcc gcgtagtctg
    10681 attttccgtg gggccattac cggtgtagat accagcaaag aagggctgca attctatgaa
    10741 gtggttcctg ttgcattagt ggttgcgggg acgcaaatgg ctacaggcca ccgtaccatg
    10801 gatactcgcc tctattttga aggtgagctg attgatgcag cgactaataa accggttatc
    10861 aaagtcgttc gtcagggcga aggtaaagac ctgaataacg aaagtacgcc aatggctttc
    10921 gaaaatatta aacaagttat tgatgacatg gcgaccgatg ccaccatgtt tgacgttaac
    10981 aaaaagtagt tccagacgcg ccatcgttag atggcgcttt ttatccggtg cgccgtaaaa
    11041 ccccatcctt cagggcgggg atataaggcg cggttttcca cctgaccagg tgtttgctgt
    11101 tgttcaatgt attggcggat gacggatatt ggcgcacctc cgcagctact ggcaaaatag
    11161 ccaggactcc acaaaacgcc tttgtagtaa tacctgactg caatatctgg tcgatctcgt
    11221 cgcagtaacc tacccgatac gcctttgagg ctgtttacca gactggatat cgccagtttg
    11281 ggaggttagt taattaacaa atggacgtga tctggttcgc catccatttc aaccagttca
    11341 gcttcaaaat aagcacatac atttgaaaag taagtgcgta gtttttctgt cgcgtcatgg
    11401 tcaaaaatct ggcgtcggta tctggtgaca aagactaggt gaacatgcat caggaaaaca
    11461 caatgccttc cacgccggat atcggtttct tttttcacag accaaagtat catttgacct
    11521 gtgaaacgat tacaggcatt taaattccag ttaagaccag gtggtcaaca ggagtgtgaa
    11581 atgaggcgct tcgcaggcgc atgtcgtttc gttttcaatc gtgctctggc acgtcagaat
    11641 gagaatcatg aggccggtaa taaatacatc ccttacggga aaatggcttc ctggctggtt
    11701 gagtggaaaa atgccactga aacgcaatgg cttaaagatt ctccctcaca gccattgcaa
    11761 cagtcactga aagaccttga gcgggcttac aaaaacttct tccggaagcg ggctgctttt
    11821 ccccgattca aaaagcggtg acagaatgat gcattccgct acccgcaggg cgttaagctc
    11881 gatcaggaaa acagccgtat ttttctgccg aaactgggct ggatgcgcta ccggaatagc
    11941 cgtcaggtca cgggtgttgt gaaaaatgtc actgtcagcc agtcctgcgg tacgtggtac
    12001 atcagtattc agacagaaag tgaagtatcc actccggctc acccttcagc atcaatggtc
    12061 gggctggatg ctggcgtggc taaactcgcc
//
LOCUS       DAJTPG010000110        12062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTPG010000110 DAJTPG010000000
VERSION     DAJTPG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO230_003281"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO230_003281"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO230_003282"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO230_003282"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5692929.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO230_003283"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO230_003283"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5692930.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO230_003284"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO230_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO230_003285"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO230_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5692931.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO230_003286"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO230_003286"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5692932.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO230_003287"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO230_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5692933.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO230_003288"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO230_003288"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5692934.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO230_003289"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO230_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5692935.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO230_003290"
     CDS             9553..10332
                     /locus_tag="OO230_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5692936.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO230_003291"
     CDS             10322..11131
                     /locus_tag="OO230_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5692937.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO230_003292"
     CDS             11218..11652
                     /locus_tag="OO230_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5692938.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="OO230_003293"
     CDS             11649..>12062
                     /locus_tag="OO230_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5692939.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2878 a         3251 c         3078 g         2855 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggt gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DAJTPG010000111        12021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTPG010000111 DAJTPG010000000
VERSION     DAJTPG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..493)
                     /locus_tag="OO230_003294"
     CDS             complement(<1..493)
                     /locus_tag="OO230_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5692940.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO230_003295"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO230_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5692941.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO230_003296"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO230_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5692942.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="OO230_003297"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="OO230_003297"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5692943.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO230_003298"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO230_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5692944.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="OO230_003299"
     CDS             3647..4156
                     /locus_tag="OO230_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5692945.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO230_003300"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO230_003300"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5692946.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="OO230_003301"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="OO230_003301"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5692947.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="OO230_003302"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="OO230_003302"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5692948.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="OO230_003303"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="OO230_003303"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5692949.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="OO230_003304"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="OO230_003304"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5692950.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="OO230_003305"
     CDS             complement(8798..9691)
                     /locus_tag="OO230_003305"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5692951.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="OO230_003306"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="OO230_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="OO230_003307"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="OO230_003307"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5692952.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12021
                     /locus_tag="OO230_003308"
     CDS             11581..>12021
                     /locus_tag="OO230_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HCR5692953.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLF"
BASE COUNT         2917 a         3115 c         3070 g         2919 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt t
//
LOCUS       DAJTPG010000112        11772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTPG010000112 DAJTPG010000000
VERSION     DAJTPG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..2341)
                     /locus_tag="OO230_003309"
     CDS             complement(<1..2341)
                     /locus_tag="OO230_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5692954.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="OO230_003310"
     CDS             complement(2275..2898)
                     /locus_tag="OO230_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5692955.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="OO230_003311"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="OO230_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO230_003312"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="OO230_003312"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5692956.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="OO230_003313"
     CDS             7506..8228
                     /locus_tag="OO230_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5692957.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11772)
                     /gene="yfaL"
                     /locus_tag="OO230_003314"
                     /pseudo
     CDS             complement(8356..11772)
                     /gene="yfaL"
                     /locus_tag="OO230_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2716 a         3270 c         2917 g         2869 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg tt
//
LOCUS       DAJTPG010000113        11732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTPG010000113 DAJTPG010000000
VERSION     DAJTPG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            128..997
                     /locus_tag="OO230_003315"
     CDS             128..997
                     /locus_tag="OO230_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5692958.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="OO230_003316"
     CDS             complement(1200..1553)
                     /locus_tag="OO230_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5692959.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="OO230_003317"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="OO230_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5692960.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="OO230_003318"
     CDS             complement(3381..3674)
                     /locus_tag="OO230_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5692961.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="OO230_003319"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="OO230_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5692962.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="OO230_003320"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="OO230_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5692963.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="OO230_003321"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="OO230_003321"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5692964.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /gene="dcuA"
                     /locus_tag="OO230_003322"
     CDS             7589..8890
                     /gene="dcuA"
                     /locus_tag="OO230_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5692965.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="OO230_003323"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="OO230_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5692966.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /gene="dsbD"
                     /locus_tag="OO230_003324"
     CDS             9320..11017
                     /gene="dsbD"
                     /locus_tag="OO230_003324"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5692967.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="OO230_003325"
     CDS             11054..11629
                     /locus_tag="OO230_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5692968.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2816 a         3030 c         2885 g         3001 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgactg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcatccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaagcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtac agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg acgctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcctt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc
     7561 aacttgttaa aaaacaagga aggctaatat gctagttgta gagctcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccacgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgag cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct agcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggcc gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcaaacgcat ctcaacttta cacaaatcaa
    10681 aacggtagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgctgccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc cc
//
LOCUS       DAJTPG010000114        11682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTPG010000114 DAJTPG010000000
VERSION     DAJTPG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..346
                     /locus_tag="OO230_003326"
     CDS             <1..346
                     /locus_tag="OO230_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5692969.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO230_003327"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO230_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5692970.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO230_003328"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO230_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5692971.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="OO230_003329"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="OO230_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5692972.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO230_003330"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO230_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5692973.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO230_003331"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO230_003331"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5692974.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="OO230_003332"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="OO230_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5692975.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="OO230_003333"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="OO230_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5692976.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="OO230_003334"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="OO230_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5692977.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="OO230_003335"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="OO230_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5692978.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="OO230_003336"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="OO230_003336"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5692979.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="OO230_003337"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="OO230_003337"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5692980.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="OO230_003338"
     CDS             complement(8961..9236)
                     /locus_tag="OO230_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5692981.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="OO230_003339"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="OO230_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5692982.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="OO230_003340"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="OO230_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5692983.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="OO230_003341"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="OO230_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5692984.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="OO230_003342"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="OO230_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5692985.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="OO230_003343"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="OO230_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2872 c         3087 g         2956 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 cgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAJTPG010000115        11579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTPG010000115 DAJTPG010000000
VERSION     DAJTPG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(272..347)
                     /locus_tag="OO230_003344"
     tRNA            complement(272..347)
                     /locus_tag="OO230_003344"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(312..314),aa:Ile,seq:cat)
     gene            906..3158
                     /locus_tag="OO230_003345"
     CDS             906..3158
                     /locus_tag="OO230_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5692986.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3494..4426
                     /gene="csiD"
                     /locus_tag="OO230_003346"
     CDS             3494..4426
                     /gene="csiD"
                     /locus_tag="OO230_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5692987.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4446..5714
                     /gene="lhgO"
                     /locus_tag="OO230_003347"
     CDS             4446..5714
                     /gene="lhgO"
                     /locus_tag="OO230_003347"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5692988.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5737..7185
                     /gene="gabD"
                     /locus_tag="OO230_003348"
                     /pseudo
     CDS             5737..7185
                     /gene="gabD"
                     /locus_tag="OO230_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7193..8479
                     /gene="gabT"
                     /locus_tag="OO230_003349"
                     /pseudo
     CDS             7193..8479
                     /gene="gabT"
                     /locus_tag="OO230_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8717..10117
                     /gene="gabP"
                     /locus_tag="OO230_003350"
     CDS             8717..10117
                     /gene="gabP"
                     /locus_tag="OO230_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5692989.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10138..10800
                     /gene="csiR"
                     /locus_tag="OO230_003351"
     CDS             10138..10800
                     /gene="csiR"
                     /locus_tag="OO230_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5692990.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10801..11250)
                     /gene="kbp"
                     /locus_tag="OO230_003352"
     CDS             complement(10801..11250)
                     /gene="kbp"
                     /locus_tag="OO230_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5692991.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11334..11492)
                     /locus_tag="OO230_003353"
     CDS             complement(11334..11492)
                     /locus_tag="OO230_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5692992.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2976 a         2844 c         2887 g         2872 t
ORIGIN      
        1 tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcaatttgc
       61 ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga tgaataaata
      121 cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca cccaccgcac
      181 ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac cccttgtacc
      241 cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact tgaaccagcg
      301 accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta agtatgcaat
      361 aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat aacagatttt
      421 tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg acaccgcctc
      481 cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc actatctata
      541 gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat aactttatta
      601 cattagctga agagttttcg catcattatg atatctgtta cttttcactc cataaaaata
      661 aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt agtttcgtat
      721 gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac agatttaaac
      781 attatccttt tccatataga tattggataa aaataggaca tctgtttgca attactttca
      841 caacaattaa acatttttat gttttcgtat acatcatatt actctaccat tagaggaacg
      901 ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac cccacattgt
      961 tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta gaatatctaa
     1021 actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta aaagacagag
     1081 aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa aacgattata
     1141 aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca cagagtagca
     1201 ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga gatacaatgg
     1261 ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat ttcactcaca
     1321 ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt aacattcctg
     1381 actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc ggacagcaag
     1441 aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag cgtaaatctc
     1501 ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct ggcgaagcga
     1561 ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct gagtttgcac
     1621 gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc gacggaccag
     1681 aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa gatctttgta
     1741 ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa gatgctaaaa
     1801 aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc gggggaagtg
     1861 gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt aaagatatag
     1921 aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt aaagatccag
     1981 taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca aaagacttcg
     2041 aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca ttaaaagaaa
     2101 agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt atgttgcaaa
     2161 atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat gatgcagcaa
     2221 aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa aattataatg
     2281 agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc gttatgaatt
     2341 acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg ctgctttatg
     2401 aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata aaagcctttt
     2461 cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc tcaagcatcc
     2521 catcagaaag acggatattg attaacatta accacgattt tcctaacaat ggcaatcttt
     2581 tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca tatatggccg
     2641 ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa tcaacgactg
     2701 aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt aataaaggtt
     2761 gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat aaagcaggaa
     2821 tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt agtcgtgggg
     2881 attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat aaaggaaacg
     2941 aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa aacatgggga
     3001 gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct tgtaaagtat
     3061 ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct cttcttttag
     3121 ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata tctgccccga
     3181 taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg aagccattta
     3241 ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa cctgttgctt
     3301 tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt tactaatttg
     3361 ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt tcagaaatgt
     3421 agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac atctcataag
     3481 aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg attcaggcca
     3541 ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc tggaactcac
     3601 cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc
     3661 gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc
     3721 gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt
     3781 gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga agctggcaac
     3841 ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc
     3901 gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc cgcaccgcgt
     3961 gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg tgctgatgat
     4021 gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg cgcgtcgccc
     4081 gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt
     4141 cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg tccagccaaa
     4201 agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa ccagcaaagg
     4261 cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc tgttctggct
     4321 gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg
     4381 tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc aaaggaattg
     4441 agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg accgccatgc
     4501 aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg
     4561 cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat tacacgcccg
     4621 gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc ttttgcgatc
     4681 aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct cagctcgaaa
     4741 tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag cgcgagtgga
     4801 taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt ggcatttttg
     4861 tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca aaaatcttcc
     4921 aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt gagcataaaa
     4981 acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg ctgattagct
     5041 gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg ggctttatta
     5101 tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag attgttaacc
     5161 acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat ctcacccgca
     5221 tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct
     5281 atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg tcggggattc
     5341 gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac tcgctgtgca
     5401 aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta agcgatctcc
     5461 agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag ctgattgacg
     5521 attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc tccccggcag
     5581 cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa
     5641 gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc ttacacgccg
     5701 catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga cagtaactta
     5761 ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg tgaagtcatc
     5821 gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat gggcgcagat
     5881 gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc
     5941 gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca gcatcaggac
     6001 gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa
     6061 atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg tatttatggc
     6121 gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca gccgattggc
     6181 gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg taaagccggt
     6241 ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac gccgttctct
     6301 gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg
     6361 gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa
     6421 ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg cgcgaaagac
     6481 atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc
     6541 gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc
     6601 tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa
     6661 ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg cgtcaccatc
     6721 gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg
     6781 gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg caacttcttc
     6841 cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga agagacgttc
     6901 ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc acaagccaat
     6961 gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg cgtcttccgc
     7021 gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat ttccaatgaa
     7081 gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc
     7141 atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga gaatgcgaag
     7201 gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg gcgttgggca
     7261 aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg tttaaggccg
     7321 tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc tgcatccgaa
     7381 ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaggtgct
     7441 ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt
     7501 cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat
     7561 cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt atcacgggcg
     7621 cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatggggct
     7681 gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca taagcgagga
     7741 tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg aagatatcgc
     7801 cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt cgccagcctt
     7861 tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt
     7921 gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg gcgtggcgcc
     7981 ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg gcgtcaccgg
     8041 gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct atgccggtaa
     8101 cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct
     8161 gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga tagccgaaaa
     8221 acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg agctgtttga
     8281 agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg
     8341 cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc gcatccttgt
     8401 accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg gtcagtgttt
     8461 tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc gtcttgtccg
     8521 gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa
     8581 caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa aagaatcaat
     8641 aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact
     8701 ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg gcgggctgaa
     8761 atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg
     8821 ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc tgttcgccgg
     8881 attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca cgcccgatac
     8941 cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct ataccatcgg
     9001 ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca tcgccgccat
     9061 gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg tcattaccct
     9121 cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct
     9181 ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag
     9241 cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata gcggcggctt
     9301 tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt tctcgtttat
     9361 gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa aacatattgt
     9421 ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt
     9481 cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat cttatcgctc
     9541 ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg tgatattact
     9601 ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc tctactcctt
     9661 aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta
     9721 cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga actattacgc
     9781 gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc tgctggttta
     9841 tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga
     9901 aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg gctttattac
     9961 ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga tctccaccgg
    10021 cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga aaaagctggt
    10081 attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg
    10141 accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt
    10201 caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact tggcgttggg
    10261 ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa
    10321 aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc
    10381 gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga tgaatgggag
    10441 gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga cgccagcgag
    10501 aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat tgtggcgggc
    10561 tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat
    10621 cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga taaacacgat
    10681 cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc cagtgagtta
    10741 atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa
    10801 ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc tttttagcat
    10861 cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga cctgtttgga
    10921 aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag tggctggtgt
    10981 cgccgttttc acctgatcat caacactggc aataccggaa atattcccca ccgcaacaag
    11041 gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc
    11101 aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat gctcctgcac
    11161 cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga gtttttctcc
    11221 ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca
    11281 gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg
    11341 cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag tgtcagcaga
    11401 atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat cggcggcaga
    11461 atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa aattactcat
    11521 tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct gccagtttc
//
LOCUS       DAJTPG010000116        11226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTPG010000116 DAJTPG010000000
VERSION     DAJTPG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..484)
                     /locus_tag="OO230_003354"
     CDS             complement(<1..484)
                     /locus_tag="OO230_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5692993.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     S"
     gene            complement(596..1624)
                     /gene="malI"
                     /locus_tag="OO230_003355"
     CDS             complement(596..1624)
                     /gene="malI"
                     /locus_tag="OO230_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5692994.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLSVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1799..3391
                     /gene="malX"
                     /locus_tag="OO230_003356"
     CDS             1799..3391
                     /gene="malX"
                     /locus_tag="OO230_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5692995.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3401..4573
                     /gene="malY"
                     /locus_tag="OO230_003357"
     CDS             3401..4573
                     /gene="malY"
                     /locus_tag="OO230_003357"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5692996.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4677..5678
                     /gene="add"
                     /locus_tag="OO230_003358"
     CDS             4677..5678
                     /gene="add"
                     /locus_tag="OO230_003358"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5692997.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5714..6754)
                     /locus_tag="OO230_003359"
     CDS             complement(5714..6754)
                     /locus_tag="OO230_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5692998.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6997..7122
                     /gene="blr"
                     /locus_tag="OO230_003360"
     CDS             6997..7122
                     /gene="blr"
                     /locus_tag="OO230_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5692999.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7395..7610
                     /gene="ydgT"
                     /locus_tag="OO230_003361"
     CDS             7395..7610
                     /gene="ydgT"
                     /locus_tag="OO230_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5693000.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7696..8136
                     /locus_tag="OO230_003362"
     CDS             7696..8136
                     /locus_tag="OO230_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5693001.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8213..8794
                     /gene="rsxA"
                     /locus_tag="OO230_003363"
     CDS             8213..8794
                     /gene="rsxA"
                     /locus_tag="OO230_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5693002.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8794..9372
                     /gene="rsxB"
                     /locus_tag="OO230_003364"
     CDS             8794..9372
                     /gene="rsxB"
                     /locus_tag="OO230_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5693003.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9365..>11226
                     /gene="rsxC"
                     /locus_tag="OO230_003365"
     CDS             9365..>11226
                     /gene="rsxC"
                     /locus_tag="OO230_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5693004.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2620 a         2897 c         2838 g         2871 t
ORIGIN      
        1 atgatgcata ggaagtcatg tttatatttt tattttctgc cgccatagaa gtgatggtca
       61 gaataacgcc acctccattt ttttccattt ctggcgcaac aagttgtgac agatggaaaa
      121 aagaaaacac attcagttca taagcacggc gaaaatctgc cattggcata tcaaacggtt
      181 taggaccacc gccaccggcg ttgttaacca ggatatcaac tttacccaga ttactgacgg
      241 caaagtctgc cagtgcagag agttcctgtt cggaagtaat atcacaacgc caggcaaatg
      301 cctgaccatc cagttgttga atttcgtcta caacatggtt agctgcgtcg gcgttaatat
      361 cactgaccac cacagatgcg ccagctgtcg cgaatgtaat ggcgatttct ttaccaatac
      421 ctgcacccgc acctgtgatg atggcgcatt ttccgtcgag tctcaggttg tcagaattaa
      481 acacataaac ctcctgtgaa cttcagtaaa aataaaaggc accgcctgtg tgccacattt
      541 tttatcatta accttgagga aaggacacta ccaagtatag cagataaaga gaggattatt
      601 tcgccgcaat gagccgggcg ggaataataa gattgcgtga atgcgtctct tcatgggtga
      661 ttttttgcat catgcgatcc gcaagtgtga taccaagttc ccgcgctggc gtgctggccc
      721 aggtaacagg tatatcatca agtgtggttg gtgtcgcatc ggtaaatgcc gccagcgaaa
      781 cctgttgctc aaagtaacga tcgactccgc tttccccgct ttgccgccct gctttcagca
      841 aaccaaacca tgcccccatc gcaatagttt cgttatagca aaccacggca ctgatggtcg
      901 ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg cttctggctg gaagtgcact
      961 ccaacaccca atcgctgtga aacggcaggc caaattttag tagagttgca caatagcccc
     1021 ccacccgttc tgcacgggtt aatgaggaac tttgccctcc cagccaggcg atccgctgat
     1081 gcccattgcg aatgagatgc tccgtcaaca actgtgcagc ctgcatgttg tccgggcgaa
     1141 ccgtatcaac atcatcaaga taactggcac gggaagcgaa aatcaccggg atagcttttt
     1201 cttctgccat ccgccgcagg tcatcgctgc ttcctgcagc cccggcaatt accacaccat
     1261 cgacaccctg attcagtaac agtgaaaacc gctgtgccag ctgctcgccg tctttaccgc
     1321 cgtgaagcaa aaaaaccatc cgtccctgtg cttccagagc ttccgtcaat ccggccgtca
     1381 attcggcgta aaacggcgca gataaatcac ggacgctcaa accaatgacg ccgctttgcc
     1441 cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc cagctcttca atggcggcgt
     1501 taacgcgttc tcctgtggcg gtagagattc gccctttgcc attaagcacc agcgaaacgg
     1561 tacttaccga cacgcccgca gccagcgcaa catcatgaat ggttattttt ttggcggtag
     1621 ccatgcagat gacctactcc ctgattatgt gacagataaa acgttttacc ttttatttat
     1681 cttatacccg ctattatcgt tgcgtaatgt gatttatgac tcactaaaat ttgataaaac
     1741 gttttatctt ctcgcgcaat ttactgaatc cagattgttc tctacgagga gtcgttttat
     1801 gacggcgaaa acagcaccga aagtcacgct gtgggagttc ttccagcagt taggcaaaac
     1861 cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt atgctcggca ttggtagttc
     1921 tcttagcagc catgatgtca taaccctgat cccggtcctg ggcaaccccg tgttgcaggc
     1981 tatctttacc tggatgagta agattggctc gtttgctttt agtttcctgc ctgtcatgtt
     2041 ctgtatcgcc atcccgctgg gcctggcacg cgaaaataaa ggcgtagcgg cattcgctgg
     2101 cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc tggttgacca ataaaggcat
     2161 tctgccaacc acagatgccg ccgttctgaa agccaataac atccagagca ttcttgggat
     2221 ccagtcgatc gataccggga tcctcggtgc ggtgatcgcc ggtattatcg tctggatgct
     2281 gcatgagcgt ttccataata tccgcctgcc ggatgcgctg gcattcttcg gcggtacgcg
     2341 cttcgtacca attatctcct cgctggtgat gggccttgtc ggcctggtga ttccattagt
     2401 ctggccgatt ttcgccatgg gtattagcgg cttgggccat atgataaaca gcgcgggtga
     2461 tttcggaccg atgctgtttg gtaccggtga acgtctgctg ttgccgtttg gtctgcatca
     2521 cattctggtg gcattaattc gctttaccga cgcaggcggc acgcaggagg tctgcggtca
     2581 aaccgtcagc ggcgcactga ccatcttcca ggcgcaattg agttgcccga ccactcacgg
     2641 tttttctgaa agcgtcacgc gtttcctttc gcaaggtaaa atgcctgcgt ttctcggcgg
     2701 tctaccaggt gcagcgttag ctatgtatca ctgcgcgcgc ccggaaaatc gccataaaat
     2761 taaaggtctg ctgatttctg gcctgatcgc ctgcgtcgtt ggcggcacta ccgaaccgct
     2821 ggaattcctg ttcctgttcg tagcgccagt tctgtatgtc atccacgcgc tgttaaccgg
     2881 cctcggcttc accgtcatgt ctgtgctcgg cgtcaccatc ggtaataccg acggcaatat
     2941 catcgacttc gtggtgttcg gtattttgca tggtctgtca accaagtggt acatggtgcc
     3001 agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc ttccgtttcg ctatcacccg
     3061 cttcaatctg aaaaccccgg ggcgcgatag cgaagttgcc agctcaatcg aaaaagccgt
     3121 tgccggtgcg ccgggtaaat caggttacaa cgttcctgca atcctcgaag cattaggcgg
     3181 tgccgacaat attgtcagcc tcgataactg cattacccgt ctgcgtttgt ctgtggaaga
     3241 tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg gcaattggcg tagtacaact
     3301 taatcaacat aacctgcagg ttgttatcgg gccacaagtt cagtcagtaa aagatgaaat
     3361 ggccggtctg atgcatactg tccaggcata aggataagat atgttcgatt tttcaaaggt
     3421 cgtggatcgt catggcacat ggtgtacaca gtgggattat gtcgctgacc gtttcggcac
     3481 tgctgacctg ttaccgttca cgatttcaga catggatttt gccactgccc cctgcattat
     3541 cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc tacagccgct ggaaaaacga
     3601 tgagtttctc gcggctattg cccactggtt ttccacccag cattacaccg caattgatac
     3661 gcagacggtg gtgtatggcc cttctgtcat ctatatggtt tcagaactga ttcgtcagtg
     3721 gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc tatgacgcat tttacaaggc
     3781 cattgaaggt aaccagcgca cagtaatgcc cgttgcttta gagaagcagg ctgacggctg
     3841 gttttgcgat atgggcaagc tggaagccgt tttggcgaaa ccagaatgta aaattatgct
     3901 cctgtgtaac ccacagaatc ctaccgggaa agtgtggacg tgcgatgagc tggagatcat
     3961 ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc gatgaaatcc atatggatat
     4021 ggtctggggc gagcagccgc atattccctg gagtaatgtg gcgcggggag actgggcgtt
     4081 gctgacatcg ggatcgaaaa gtttcaatat tcccgcgctg accggtgctt acgggattat
     4141 agaaaatagc agcagccgtg atgcctattt atcggcactg aaaggacgtg atgggctttc
     4201 ttccccttcg gtactggcgt taactgccca tatcgccgcc tatcagcaag gtgcaccgtg
     4261 gctggatgcc ttacgcgtct atctgaaaga taacctgacg tatatctcag ataaaatgaa
     4321 cgccgcgttt cctgaactca actggcagat cccgcaatcc acttatctgg cctggcttga
     4381 tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa gcgcttatcg agcaagaaaa
     4441 agtcgcgatc atgccggggt atacctatgg tgaagaaggt cgtggttttg tccgtctcaa
     4501 tgccggctgc ccacgttcga aactggaaaa aggtgtggct ggattaataa acgccatccg
     4561 cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt tgcgcaatcg tggattttta
     4621 ccctgctttg tttttataat ggtgcgcact tttatatcca gaaaaagagt gcgaccatga
     4681 ttgataccac cctgccatta actgatatcc atcgccacct tgatggcaac attcgtcccc
     4741 agaccattct tgaacttggc cgccagtata atatctcgct tcctgcacaa tcccttgaaa
     4801 cactgattcc ccacgttcag gtcattgcca acgaacccga tctggtgagc tttctgacca
     4861 aacttgactg gggcgttaaa gttctcgcct ctcttgatgc ctgccgccgc gtggcatttg
     4921 aaaacattga agatgcagcc cgtaacggcc tgcgctatgt cgagctgcgt ttttcaccag
     4981 gctacatggc aatggcacat aagctgcctg tagcgggtgt tgtcgaagcg gtgatcgatg
     5041 gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa gcttatcggc attatgagcc
     5101 gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc ctttttagcc caccgtgacc
     5161 agattaccgc acttgattta gctggtgatg aacttggatt cccgggaagt ctgttccttt
     5221 ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac cgtccatgca ggcgaagctg
     5281 ccgggccgga aagcatctgg caggcgattc gtgaactggg tgcggagcgt attggacatg
     5341 gcgtaaaagc cattgaagat cgggcgctga tggattttct cgccgagcaa caaattggta
     5401 ttgaatcctg tctgacctcc aatattcaga ccagcaccgt agcagagctg gctgcacatc
     5461 cgctgaaaac gttccttgag catggcattc gtgccagcat taacactgac gatcccggcg
     5521 tacagggagt ggatatcatt cacgaatata ccgttgccgc gccagctgct gggttatccc
     5581 gtgagcaaat ccgccaggca cagattaatg gtctggaaat ggctttcctc agcgcagagg
     5641 aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca aaaatggatg gtgcaaattg
     5701 caccatccat ttttcacgca aggcacaacg tcgcgcgatg tttggcggat tcgatgccca
     5761 gctcaatcaa ctccattacc tggattgcct ggcttgccgg aaccggattt tcaccatcgc
     5821 catttaacgc atcacgaatc gccgcatagt aagccggata attcccaggc accgtcaaca
     5881 gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag tacgccatca cgcatatcgt
     5941 agccccagtc ttcctgcggc agacgctcgc catttttcag acgttcttcc tgtggatcga
     6001 ggccatattt cacataactg cctcgggatc catgcacgat ataccgtgct gactcagcgg
     6061 ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg ataggacaag atggcgtgga
     6121 aataatcggt cgactgcgct ccgggccgta actgtgccaa atctaccgtc atgctgaccg
     6181 gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg tgctaaatcg taccagatac
     6241 cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac ctgcggacgg aagcggtcaa
     6301 aatgagactc aaagtaagca acttcaccca gcacgccttc cgcgagcaaa ccttttagcg
     6361 tcaagaaatc gctatcccaa cggcggttat ggaatacaga cagcacacgc cccaggcttt
     6421 ttgccagcgc atccagctct cgcgcttgtg acagtgtcac ggtaaagggt ttatcaacga
     6481 ccacatgttt acccgcctca agcgccgctt tggctaacgg gaaatgggta tcgttgggtg
     6541 taggaatgac aatcaggtct atgttgggat cgttaaacag atgcttcggc tcagagacaa
     6601 ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc actgctggag attaccgcca
     6661 gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa ggttttgctc gcataaccat
     6721 acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt gcctctcatt gaagtatgat
     6781 ggctatttga cactatcctt tacccacgct caacagttta ataacctgcc agcaataagg
     6841 gatgttgttt aacttaagtc aaaaaaatag cgaattttcc aacgacaaaa gctaaatatc
     6901 gcaaaaacct cagtaaaaat cttgctggag ctattattgc taagtaacat ttaccccctg
     6961 aagttaatgg atcaatcaag agagatgtgg gctgtaatga atcgtcttat tgaattaaca
     7021 ggttggatcg ttcttgtcgt ttcagtcatt cttcttggcg tggcgagtca cattgacaac
     7081 tatcagccac ctgaacagag tgcttcggta caacacaagt aagctctgca cttgtggagc
     7141 gacatgctgc ccgtccgggt gcatgttttc acttgtcgga tattaaacca ggaatttatt
     7201 atcttgttcg atgttgttgg tgattgtcag ggatagtaaa gcgcgttact cttttcagag
     7261 cagtttttca tccctgaata tttgccatca gcgcattctt attattgtaa taacacaagc
     7321 gttcactttt ggtgaaattt tatcatcact aataatacct acccctttgg tattaattat
     7381 tttatggacc ttttatgact gttcaggact acttattaaa atttcgcaaa atcagttcac
     7441 tcgaaagtct ggaaaaactc tacgaccatc ttaattacac cctgacggac gatcaggaac
     7501 tgatcaatat gtatcgtgct gccgatcacc gtcgcgcaga gctggtttct ggcgggcgtt
     7561 tgtttgacct cggccaggta ccgaagtccg tctggcacta tgtccaataa agaaagtagc
     7621 gatcggttat aaaagcctta taataacgcc cctaaaatta gatttgcctg tttgcgtcgc
     7681 tattctggag aacctatgac caccacaacg ccacaacgta ttggaggctg gttgcttggc
     7741 ccgttggcct ggcttttagt cgctctgttg agtacgacgc tggcgttgtt gctgtatacc
     7801 gctgcgttat cttctccgca aacatttcaa acgctcggcg gacaagcact gacgacacaa
     7861 atcttatggg gcgtttcttt tattaccgct atcgccatgt ggtattacac actgtggttg
     7921 accatcgctt tctttaaacg tcggcgttgt gttcctaaac actatattat ctggctgctt
     7981 atttccgtat tgctggcagt aaaagcattc gctttttcac ccgtggaagt tggcatagcc
     8041 gttcgccagt tgctgtttac tttgttggca accgcattga tcgtccccta tttcaaacgc
     8101 tcgtcgcggg taaaagcgac gtttgtgaat ccgtaataac cttacagtta acctgttgtc
     8161 gcctgctctg gattaacgga taataggcgg cttttttatt tcaggccgaa aaatgactga
     8221 ctacctgtta ctgtttgtcg gaactgtact ggtcaataac tttgtactgg tcaagtttct
     8281 cggtctctgt ccgtttatgg gggtttccaa aaaactggaa accgcgatgg gcatggggct
     8341 ggcaacaacg tttgtgatga cgctgacgtc tatttgcgcc tggcttatcg atacgtggat
     8401 tttgatccca cttaatctga tttacctgcg caccctggca tttattctgg tgattgctgt
     8461 ggtcgtgcag ttcaccgaga tggtggtgcg caaaaccagc ccggtgcttt accgtctgct
     8521 ggggattttt ttgccgctta tcaccaccaa ctgtgcagtg ctcggcgtgg cgttgctgaa
     8581 tatcaatctc gggcacaatt tcttgcagtc ggcgctgtac ggtttttccg ccgctgtcgg
     8641 tttctcgctg gtgatggtgc tcttcgccgc catccgcgaa cgccttgctg tggctgatgt
     8701 cccggcacct tttcgcggta atgccattgc gttaattacc gcaggtctta tgtctctggc
     8761 ctttatgggc tttagtggtt tggtgaagtt gtaatgaatg ctatctggat tgccgttgcc
     8821 gccgtgagcc tgctgggcct ggcgtttggc gccattctgg gttatgcctc ccgccgtttt
     8881 gcggtggaag acgatccggt cgttgagaaa attgacgaaa tcttaccgca gagccagtgt
     8941 ggtcagtgcg gttatcccgg ctgtcgcccc tacgcggaag ccatcagctg taacggtgaa
     9001 aaaatcaacc gttgcgcccc aggtggcgaa gctgtgatgc taaaaattgc cgagttgctt
     9061 aatgtcgagc cgcagccgct ggatggcgaa gcgcaagagc taacgcctgc gcggatggtg
     9121 gcggttattg atgaaaataa ctgtattggc tgcactaaat gtattcaggc ttgtccggta
     9181 gacgccatcg ttggcgctac ccgtgtcatg catacggtaa tgagtgatct ctgtacgggc
     9241 tgcaatttat gtgttgatcc gtgcccgacg cactgcatct cgttgcaacc ggtcgcagaa
     9301 acacctgact cctggaaatg ggatctgaac accattcccg tgcgtatcat tcccgtggaa
     9361 caccatgctt aagttattct ctgcattcag aaaaaataaa atctgggatt tcaacggcgg
     9421 catccatcca ccggagatga aaacccagac caacggtacc cccctgcgcc aggtactact
     9481 ggcgcagcat tttgttattc cactgaaaca gcatattggc gctgaaggtg agttgtgcgt
     9541 tagcgtcggc gataaagtat tgcgcggcca gccgcttacc cgtggtcgcg gcaaaatgct
     9601 gcctgttcac gcgcccacct cgggtaccgt tacggctatt gcgccccact ctacggctca
     9661 tccttcagct ttagctgaat taagcgtgat tattgatgcc gatggtgaag actgctggat
     9721 cccgcgcgac ggctgggtcg attatcgctc tcgcagtcgc gaagagttaa tcgagcgcat
     9781 acatcagttt ggtgttgccg ggctgggcgg tgccggattc ccgacaggcg ttaaattgca
     9841 gggtggtgga gataagattg aaacgttgat tatcaacgct gctgagtgcg agccgtacat
     9901 taccgccgat gaccgtttga tgcaggattg cgcggctcag gtcgtagagg gtattcgcat
     9961 tcttgcgcat attctgcagc cacgcgaaat tcttatcggc attgaagata acaaaccgca
    10021 ggcgatttcc atgctgcgcg cggtgctggc ggactctaac gatatttctc tgcgggtgat
    10081 tccaaccaaa tatccttctg gcggtgctaa acaattaacc tacattctga ccgggaagca
    10141 ggttccacat ggcgggcgtt catccgatat cggcgtatta atgcaaaacg tcggcactgc
    10201 ttatgcagtg aaacgtgccg ttattgatgg cgagccgatt accgagcgtg ttgtaaccct
    10261 gactggcgaa gcaatcgctc gcccgggcaa cgtctgggca cggctgggga cgccagtgcg
    10321 tcatttattg aatgatgccg gattctgccc ctctgccgat caaatggtga ttatgggtgg
    10381 cccgctaatg ggctttacct tgccatggct ggatgtcccg gtcgtaaaga ttaccaactg
    10441 tctgttggct ccctctgcca atgaacttgg cgaaccacag gaagaacaaa gctgcatccg
    10501 gtgtagcgcc tgtgctgacg cctgccctgt ggatcttttg ccgcaacagt tgtactggtt
    10561 cagcaaaggt cagcaacacg ataaagctac cacgcataac attgccgact gcattgaatg
    10621 tggggcttgc gcgtgggttt gcccgagcaa tattcccttg gtgcaatatt tccgtcagga
    10681 aaaagctgaa attgcggcta ttcgtcagga agaaaagcgc gccgcagaag ccaaagcgcg
    10741 tttcgaagcg cgccaggctc gtctggagcg cgaaaaagcg actcgccttg aacgacataa
    10801 gagcgcagcc gttcaacctg cagccaaaga taaagatgcg attgctgccg ctctggcgcg
    10861 ggtgaaagag aaacaggccc aggctacaca gcctattgtg attaaagcgg gcgaacgccc
    10921 ggataacagt gcaattattg cagaacggga agcccgtaaa gcgcaagcca gagcgaaaca
    10981 ggcagaactg gagcaaacta acgacgcagc aaccgttgct gaaccacgta aaactgccgt
    11041 tgaagcagct atcgcccgct ccaaagcgcg caagctggaa cagcaacagg ctaatgcgga
    11101 accagaagaa caggtcgatc cgcgcaaagc cgccgtcgaa gccgctattg cccgtgccaa
    11161 agcgcgcaag ctggaacagc aacaggctaa tgcggaacca gaagaacagg tcgatccgcg
    11221 caaagc
//
LOCUS       DAJTPG010000117        11100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTPG010000117 DAJTPG010000000
VERSION     DAJTPG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO230_003366"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO230_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5693005.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO230_003367"
     tRNA            complement(621..695)
                     /locus_tag="OO230_003367"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO230_003368"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO230_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO230_003369"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO230_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5693006.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO230_003370"
     CDS             complement(2932..3990)
                     /locus_tag="OO230_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693007.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO230_003371"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO230_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5693008.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO230_003372"
     CDS             6068..6352
                     /locus_tag="OO230_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5693009.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO230_003373"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO230_003373"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5693010.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO230_003374"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO230_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5693011.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO230_003375"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO230_003375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5693012.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2772 a         2544 c         2745 g         3039 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
//
LOCUS       DAJTPG010000118        11050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTPG010000118 DAJTPG010000000
VERSION     DAJTPG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            410..1240
                     /locus_tag="OO230_003376"
     CDS             410..1240
                     /locus_tag="OO230_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5693013.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="OO230_003377"
     CDS             complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="OO230_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5693014.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2369..3829)
                     /locus_tag="OO230_003378"
     CDS             complement(2369..3829)
                     /locus_tag="OO230_003378"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5693015.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="OO230_003379"
     CDS             complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="OO230_003379"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5693016.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5434..6777
                     /gene="gntP"
                     /locus_tag="OO230_003380"
     CDS             5434..6777
                     /gene="gntP"
                     /locus_tag="OO230_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5693017.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="OO230_003381"
     CDS             complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="OO230_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5693018.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="OO230_003382"
     CDS             complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="OO230_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5693019.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="OO230_003383"
     CDS             complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="OO230_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5693020.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9006..>11050)
                     /gene="fimD"
                     /locus_tag="OO230_003384"
     CDS             complement(9006..>11050)
                     /gene="fimD"
                     /locus_tag="OO230_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5693021.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2685 a         2829 c         2683 g         2853 t
ORIGIN      
        1 tacgtagatg aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt
       61 ccgcataatc agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa
      121 catatcaaat acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc
      181 aatatcaaat tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa
      241 tgagcgtttt ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga
      301 tatcactaca acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat
      361 ttatctattt ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc
      421 attaagcaat gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa
      481 agtcgacagt gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta
      541 tcttctgcct cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat
      601 tagaaaactt aatattggca aacagtcctg gccatttaat tatgtgacag atacatgcag
      661 agaattcaat ggtattacaa caacaggacg tatgccctat cgaaacttga aaatcacctc
      721 agctttagac gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc
      781 agaaggttta cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac
      841 agttcatgaa ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat
      901 aggatatatt tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag
      961 aattgatatc tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt
     1021 cgcccttgcc gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat
     1081 agaagcctat cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg
     1141 tgatgaagat gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt
     1201 tgaattaaaa aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac
     1261 taatattcag gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat
     1321 tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag
     1381 tcaggcgtcg actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc
     1441 gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc
     1501 cagcttcgcc gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc
     1561 caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg
     1621 attgttttcc cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt
     1681 tgcttcagca atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct
     1741 ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg
     1801 ggtagcctgc agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa
     1861 cagctcaaaa ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct
     1921 gcctgagcta tcaagcacat agataccggc accccggcgc acttccacca gccctttgat
     1981 ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat
     2041 ttcacgctcc ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgtg
     2101 gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt
     2161 gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg
     2221 cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag
     2281 ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga
     2341 ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc
     2401 gttcgcacag ctgctgatat gccgacgtca ccgcgccaac aaagtcggca ttctgcggca
     2461 gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat
     2521 actgcgcgtt aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac
     2581 cctgctcatc cacgccctgg gtgtaaccca tccagccagc cacgcccagc gccaggtgac
     2641 gccagctgcc gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact
     2701 tctggctgcc gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac
     2761 gctcgatcag cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg
     2821 gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg
     2881 tgtcggcaat ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc
     2941 cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgtgc
     3001 ccactttatc ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga
     3061 acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta
     3121 aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt
     3181 tctcttcaat ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga
     3241 ccttcgccac atggccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca
     3301 gccctttttc acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg
     3361 cagtcgggtt ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg
     3421 ccgcatcagc gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac
     3481 gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca
     3541 tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca
     3601 gttgctgttt tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt
     3661 cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt
     3721 gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat
     3781 cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca
     3841 gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc
     3901 cggtgggtat taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc
     3961 ggccaggcct ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc
     4021 gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc
     4081 tttacgacgg tgttcttctt caacaatcgc tttcaccact ttgtacatat caacgtcacc
     4141 gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag
     4201 atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc
     4261 acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc
     4321 aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg
     4381 cggcggatcg tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat
     4441 cgctttcagg aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag
     4501 ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc
     4561 aatgatgtta cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc
     4621 tgcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg
     4681 catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag
     4741 cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt
     4801 gcgaatgcca cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc
     4861 atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca
     4921 caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac
     4981 ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac
     5041 atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt
     5101 tcctctttca catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt
     5161 atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt
     5221 tctacctttt ggtaagacat gtcaaccaga attctgaaat tggttaaccg cgtcgcaaga
     5281 atttcactct catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc
     5341 agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg tcgcccaaaa
     5401 taacaacggt tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg
     5461 gtattcggca ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg
     5521 gctttgttgg tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg
     5581 ctgcacacca tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg
     5641 ttcggtgcgg tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat
     5701 acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt cggtgattat catcggcctt
     5761 attttcggtc tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt
     5821 gttattgccg ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct
     5881 gccactaccg cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct
     5941 tatggcgcgg atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta
     6001 atctgcgcag gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca
     6061 ttcctgaaag cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt
     6121 ctggtgccgc tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg
     6181 gtaaaagata cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg
     6241 tttattgcga tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag
     6301 tgggtgatga acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc
     6361 ggtgcgggtg gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc
     6421 atgttgatgt cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta
     6481 attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt
     6541 gctgcaatcc ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta
     6601 ctggcgacgg ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg
     6661 ttcaaaggtt actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag
     6721 ctggtcaact ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag
     6781 aacaccagag cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg
     6841 gacagtcccc tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg
     6901 cgctttgttt atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg
     6961 tgcaggtttt gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt
     7021 aattttttta ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca
     7081 cctgccctcc ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta
     7141 ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta
     7201 cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat
     7261 ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa
     7321 gaggaattgg cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa
     7381 catcacaacc gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact
     7441 gaaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac
     7501 cagctttaat agccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc
     7561 acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg
     7621 gatagctact gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag
     7681 ccgagcctcg ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa
     7741 agatttgcgt cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt
     7801 ttacataaac attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac
     7861 aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag
     7921 ctcgtttcat tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact
     7981 gcctgaatgg ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg
     8041 aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat
     8101 gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta
     8161 taatatccgg tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc
     8221 ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata
     8281 agactgaaag aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac
     8341 ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca
     8401 cttgcgagcg ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc
     8461 tccagttact gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca
     8521 ggcacctgtg tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc
     8581 agggtcgtcc acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca
     8641 ttcagaagct gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg
     8701 ttggcattgt ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta
     8761 ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta
     8821 aattgcttcg ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca
     8881 ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg
     8941 cgacttaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg
     9001 cccccttaac gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc
     9061 agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt
     9121 cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc
     9181 tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc
     9241 gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga
     9301 gtgggaacaa cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta
     9361 tccagcgcca ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc
     9421 cagtcggtac gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta
     9481 acaagcacca ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat
     9541 accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata
     9601 ttggcattgc cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta
     9661 ccatcgcctc ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc
     9721 aacgtaccgt atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg
     9781 tagctggcac tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa
     9841 ggaatattga cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc
     9901 aggctatagc tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat
     9961 tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt
    10021 gatgtgcgcc cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg
    10081 aggttgtaat agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg
    10141 ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa
    10201 taacggtaac ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga
    10261 aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc
    10321 atatccacag acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca
    10381 cgataacgat ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg
    10441 agtaatgtac tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct
    10501 cctgccgtaa tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag
    10561 ggtacggtaa aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca
    10621 ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta
    10681 taaatgccat atccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg
    10741 atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt
    10801 gcgccgcgaa agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc
    10861 agccgggaac gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta
    10921 tttttgctac ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa
    10981 cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc
    11041 ccaatccgat
//
LOCUS       DAJTPG010000119        10926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTPG010000119 DAJTPG010000000
VERSION     DAJTPG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            154..504
                     /locus_tag="OO230_003385"
     CDS             154..504
                     /locus_tag="OO230_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5693022.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OO230_003386"
     CDS             557..952
                     /locus_tag="OO230_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5693023.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OO230_003387"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OO230_003387"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5693024.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OO230_003388"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OO230_003388"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5693025.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO230_003389"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OO230_003389"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5693026.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OO230_003390"
     CDS             complement(5323..6423)
                     /locus_tag="OO230_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5693027.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO230_003391"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OO230_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5693028.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OO230_003392"
     CDS             complement(7571..8137)
                     /locus_tag="OO230_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5693029.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OO230_003393"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OO230_003393"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5693030.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="OO230_003394"
     CDS             10263..10751
                     /locus_tag="OO230_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5693031.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2669 a         2788 c         2605 g         2864 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAJTPG010000120        10772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTPG010000120 DAJTPG010000000
VERSION     DAJTPG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            152..1606
                     /gene="amn"
                     /locus_tag="OO230_003395"
     CDS             152..1606
                     /gene="amn"
                     /locus_tag="OO230_003395"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5693032.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            1949..2665
                     /locus_tag="OO230_003396"
     CDS             1949..2665
                     /locus_tag="OO230_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5693033.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3121..3196)
                     /locus_tag="OO230_003397"
     tRNA            complement(3121..3196)
                     /locus_tag="OO230_003397"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3161..3163),aa:Asn,seq:gtt)
     gene            complement(3297..4924)
                     /gene="yeeO"
                     /locus_tag="OO230_003398"
                     /pseudo
     CDS             complement(3297..4924)
                     /gene="yeeO"
                     /locus_tag="OO230_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4929..5004
                     /locus_tag="OO230_003399"
     tRNA            4929..5004
                     /locus_tag="OO230_003399"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4962..4964,aa:Asn,seq:gtt)
     gene            complement(5042..5992)
                     /gene="cbl"
                     /locus_tag="OO230_003400"
     CDS             complement(5042..5992)
                     /gene="cbl"
                     /locus_tag="OO230_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5693034.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6094..7011)
                     /gene="nac"
                     /locus_tag="OO230_003401"
     CDS             complement(6094..7011)
                     /gene="nac"
                     /locus_tag="OO230_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5693035.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7339..7414
                     /locus_tag="OO230_003402"
     tRNA            7339..7414
                     /locus_tag="OO230_003402"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7372..7374,aa:Asn,seq:gtt)
     gene            complement(7470..8405)
                     /gene="ldtA"
                     /locus_tag="OO230_003403"
     CDS             complement(7470..8405)
                     /gene="ldtA"
                     /locus_tag="OO230_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5693036.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8467..9546)
                     /gene="cobT"
                     /locus_tag="OO230_003404"
     CDS             complement(8467..9546)
                     /gene="cobT"
                     /locus_tag="OO230_003404"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5693037.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9558..10301)
                     /gene="cobS"
                     /locus_tag="OO230_003405"
     CDS             complement(9558..10301)
                     /gene="cobS"
                     /locus_tag="OO230_003405"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5693038.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10298..>10772)
                     /gene="cobU"
                     /locus_tag="OO230_003406"
     CDS             complement(10298..>10772)
                     /gene="cobU"
                     /locus_tag="OO230_003406"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5693039.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3012 a         2743 c         2322 g         2695 t
ORIGIN      
        1 tgatttctgc aatgaccgct ttattgatga aagacaatca acgctcgtga tagcctggcg
       61 aagatcatcc gatcttcgcc ttatactttt gtttcacatt cctgtgacat actatcggat
      121 gtgcggtaat tgtatggaac aggagacaca catgaataat aagggctccg gtctgacccc
      181 agctcaggca ctggataaac tcgacgcgct gtatgagcaa tctgtagttg ctttacgcaa
      241 cgccattggc aaatatatta caattggcga attacctgat gaaaacgccc gtaaacaagg
      301 tctttttgtc tatccatcac tgaccgtaac ctgggacggt agcacaacaa atccccccaa
      361 aacgcgcgca tttggtcgtt ttactcacgc aggcagctac accaccacga ttactcgccc
      421 tactctcttt cgttcgtatc tgaatgagca actaacgttg ctttatcagg attatggtgc
      481 gcatatctct gtgcaaccct cgcaacatga aatcccttat ccttatgtca tcgatggctc
      541 tgaattgaca cttgatcgct caatgagcgc tgatttaact cgctacttcc cgacaacaga
      601 actggcgcaa atcggcgatg aaactgcaga cggcatttat catccaacgg aattctcccc
      661 gctatcgcat tttgatgcgc gccgcgtcga tttttccctc gcacggttgc gccattatac
      721 cggtacgcca gtagaacatt ttcaaccgtt cgtcttgttt accaactaca cacgttatgt
      781 ggatgaattc gttcgttggg gatgcagcca gatcctcgat cctgatagtc cctacattgc
      841 cctttcttgt gctggcggga actggatcac tgccgaaacc gaagcgccag aagaagccat
      901 ttccgacctt gcatggaaaa aacaccagat gccagcatgg catttaatta ccgctgatgg
      961 tcagggtatt actctggtga atattggcgt gggaccgtcc aatgctaaaa ccatctgcga
     1021 tcatctggca gtgctacgcc cggatatctg gttgatgatt ggtcactgtg gcggattacg
     1081 tgaaagccag gccattggag attatgtcct tgcacacgct tatttacgcg atgaccacgt
     1141 tcttgatgcg gttctgccgc ccgatatacc tattccgagc attgctgaag tgcaacgtgc
     1201 gctttatgac gctaccaagt tagtcagcgg caggcctggt gaggaagtca aacagcggct
     1261 gcgtacaggt actgtggtaa ccacagatga caggaactgg gagttacgtt actcagcttc
     1321 tgcacttcgt tttaacttaa gccgggccgt agcaattgat atggaaagtg caaccattgc
     1381 cgcgcaagga tatcgtttcc gcgtgccata cgggacacta ctgtgtgttt cagataaacc
     1441 gttacatggt gagattaagc ttcccggcca ggctaaccgt ttttatgaag gcgctatttc
     1501 cgaacacctg caaattggca ttcgggcgat cgatttgctg cgcgcagaag gcgaccgact
     1561 gcattcacgt aaattacgaa cctttaatga gccgccgttc cgataataag ttatgaacct
     1621 tcttatcaga aatagatgtg aaggattaag taagacatct ggcaaatagc ctgcaataaa
     1681 cgcgggctgc ttgcttcctt gggcagatgc gagttttatt atcgtcttaa tgatttccac
     1741 atattaaaag caagtatgct ttcaaaacac aattataaaa aatcccgcca acaacattag
     1801 tttttataaa attaaatata agattatggc tttagaatat ttttatttct aatagacgag
     1861 atttttcctg ttatgatata atatgccgaa ttaacacatg ttaacgattt accagtaatg
     1921 taaataaatt ttcgaggaga tcattccagt gggacgtaaa tgggccaata ttgttgctaa
     1981 aaaaacggct aaagacggtg caacgtctaa aatttatgca aaattcggtg tagaaatcta
     2041 tgctgctgcc aaacaaggtg aacccgatcc agaattaaac acatctttaa aattcgttat
     2101 tgaacgtgca aagcaggcac aagttccaaa gcacgttatt gataaagcaa ttgataaagc
     2161 caaaggcggc ggagatgaaa cgttcgtaca gggacgttat gaaggctttg gtcctaatgg
     2221 ctcaatgatt attgctgaga cattgacttc aaatgttaac cgtacgattg ctaacgttcg
     2281 cacaattttc aataaaaaag gcggcaatat cggagcggca ggttctgtca gctatatgtt
     2341 tgacaatacg ggtgtgattg tatttaaagg aacagaccct gaccatattt ttgaaatttt
     2401 gcttgaagct gaagttgatg ttcgtgatgt aactgaagaa gaaggtaata ttgttattta
     2461 tactgaacct gctgaccttc ataaaggaat cgcggcgcta aaagcagctg gaatcactga
     2521 gttctcaaca acagaattag aaatgattgc tcaatctgaa gttgagcttt ccccagaaga
     2581 tttagaaatc tttgaagggc ttgttgatgc ccttgaagat gacgacgatg tacaaaaagt
     2641 ttatcataac gtcgcaaatc tctaattata ttttaaagaa atctgtcttt atggcagatt
     2701 tctttaatct catataattc ttataaaaaa tataatattt aactcgtcat attgcttata
     2761 aaaaatcccc gtccccagag aaataatatt tattaaaatg gcaggtcttc tttttctgat
     2821 taccgaagac aaagtggcaa ttacgtatag tttcccgata aagacgcgac agctacgtcc
     2881 cgcataaggc atttttctct ttatctttgt acggtacttc atggaacaga atttttgacc
     2941 ttgtgaatcg tgatatctgt tggggagggg caatttgctc actgaagcgt gagactcgat
     3001 taagcgcacg aaacacagaa atcaaaaaac ccggccactt ttttacaaag tggccgggta
     3061 aaaataattt ttatttttta actgtcctga gtctcataga gatgtctcaa aactaaaatt
     3121 tggctcctct gactggactc gaaccagtga catacggatt aacagtccgc cgttctaccg
     3181 actgaactac agaggaatcg tgagaacgag gcgaatatta gcgatgccca cccacaatgt
     3241 caaagcctgt tttttaaatt tgaaatcgtt tgctgaaata atctgcattt tgtcgtttat
     3301 tccgacacaa ctggcttttt ttcacacttt tgcggctcgg atcgagggta tttccacagc
     3361 caacgtccag taaccattcg ccagtaaaac agcacggccc gcacagccca gtcggcaaac
     3421 attcccatcc agacaccaac cacaccccag ccaagcatga ttccctgcac ataaccgact
     3481 acaacccggc aaccccacat gctcaacata gaaacccaca tggcgtaacg ggcatcacga
     3541 gcacccttaa atccagcggg tagcacccat gaggcggacc aaataggcat aaataaagca
     3601 tttagctaaa tcagaatcac aacgacgtgt ttaacctgtg gatcctgggt gtaaaacgat
     3661 gccataaccc cggcaaaggg agccgttagc caggcgatgg ccgttaatcc aagagtggac
     3721 agccagaaca catgccgcaa ctgaatctct gcttgcgcta tctgccctac ccccaacctt
     3781 cggcctgtaa tgatcgtaga agcagaaccg agcgcacttc cgggtaagtt gataagagcc
     3841 gcaattgaga acgcgataaa atttccggca ataacgctgg tccccatccc ggcaacgaac
     3901 atttgggtta ataaccgacc actggtaaat aacactgatt cgacactcgc tggaatacca
     3961 atccccatga cttcccagat aatgctaaaa ttcagcggtt taaaatagct ctttaacgaa
     4021 atccttagcg caggattaaa accaatcgcc agcacccaca aaattgcaac tgcgccaata
     4081 taacgagaaa tggttaaacc caacccagcc ccgaccagga gaaaaggccg taaatcaata
     4141 tgccgctaat aataatatta agaatattca ggctaccgtt aatcagtagc ggtattttcg
     4201 tattccctgc accacgaagt gccccgctac caataagagt gatggcagcg gctggataac
     4261 tgagtacggt cagctccaga taagtcaacg ccagtgcttt aacttctgtc gtggcatcac
     4321 ccgcgacgaa atcaataatt tgttcgccaa aatgatgaat tagcgttgcc aacagtacgg
     4381 caaacaacgt catgatcacc aatgactgcc gcgtcgccac cctcgctcgt cgtcgatccc
     4441 gcttaccgag actaaatgct acaacgacag tagtaccaag atcgatagct gcaaaaaaag
     4501 ccataatgac catattgaag ctgtccgcca accccacgcc ggccatcgca tcttttccca
     4561 gccaactgac cagaaaagtg ctcaggaccc ccatcaacag gacacaggcg ttctccatga
     4621 agataggaac agcaagcggg gttatctcgc gctagaacaa cactttgtag ctcttgcgtt
     4681 tagagtgcca gcgagtgccg tgaacaacct ggcgtaaagc agaggagata ttcaaagccg
     4741 accttaattg cagaaagtga aaccacattt caaatgatga gggagaatca gcaaagctgc
     4801 aaagattttc gccaacaaat tgtctgcaaa tgcaacaaac tgttgataga aacggcaaac
     4861 agttgggaaa tttaaaaatc gggtttgaca aaatattttt cgccgttaag atgtgcctca
     4921 acagcgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg
     4981 gttcgagtcc agtcagagga gccaaatttc tgaaaagccc gcttttatag cgggcttttt
     5041 gctatatctg ataatcaatt tcctcttcac tgttttccat cacctggcgc ttgatatcct
     5101 caactgacag tcctgcatta caaagttcca gaaagcgcca aacatagtta cgctgaagtt
     5161 gtcctcgctt cagtcccaac cagacagtat tagcatcgaa aagatgccgc gtatccaggc
     5221 ggattaaatt ctcttcctct tgttcgccgc tggattgttc ggcaactaat ccgatcccaa
     5281 gcccaagagc aacataggtt ttaatgacat cagaatcctg tgcacttaat acaatatctg
     5341 ccggcaaacc tttgcgggta aatgcgtcat cagtgcgtga gcgccccgta attccctgtc
     5401 ggtaagtaat taacggccac ttcgctattg attccagcgt caatggtgta atttgcgtta
     5461 agggatgatc gagtggaaca agcaaactat ggtgccaaca aaaccatggg aaggcgacga
     5521 gctgcgggtc attactcaaa cgctcgctgg cgataccaat atcagcttcg ccattttgca
     5581 acaacgtcgc aatttcctgt ggcgaaccct ggattagctc gagccgaacc tccgggaaaa
     5641 gttcgcgaaa agctttaatg acctctggca agctataacg tgcctgagta tgcgtcgttg
     5701 caatagtgag aacaccagac gtatcgttgg taaacaggtc tgcaagccga cgaacattac
     5761 tggcttcatt cagaatacgt tctgcaatga ccagtaatgc tttgcccggt tcagtcatcc
     5821 ccagcagtcg cttacctcgt cgaacaaata tttcgatgcc aaattcatcc tccagttccc
     5881 gaatatgacg gctaacgcct gactgtgagg taaaaagcat attcgcaacc tctgtcaggt
     5941 tgtaatcctg acgtgcagcc tcgcggatta tctttagttg ttggaaattc acggtaaact
     6001 ccgggcagtt cagatatccc gttattgtta aagtctaatg cccggcataa caaataataa
     6061 aaacccgcat cttattccat cccgatataa cgcttagctc accaattgcc actgcctttt
     6121 ttccatcact ggagaactaa tcactgacat taacaactct ttcactgcct gtgcctgtgg
     6181 tgacaagttt gctctggcgg gtaaatttaa ggacaaagag agactcattg aaggagtggt
     6241 aatgcgtgac atccacccat ttactgcgcc acataacgaa cgcgcagccg attcaggtaa
     6301 tactgcaacg cccatgccgc tggcaatcgc tgcggtaagc gtggcaatag actcaatttc
     6361 accaatgact tttgccgtga gccgccgtag ggaaaaagcc tcatcaacac gaagtctaac
     6421 agcgctgtaa tcactgggga gaaaaaggtt catttgcgca atagcattaa catcaacgtt
     6481 ttgccccggg caatcttgag ttcctaccag gaaaagatct tctttcagca aagcctgact
     6541 ggatacccca gccacagggg aatgctcata aatcaccgcc atatcgagtt ggtgatttat
     6601 cagtttttcg ttaagcactg caccactatt ttcatgaaga tagataacga cctccgggaa
     6661 ttcagcgcga accgcctgta ataagggcat ggtgatggat gacgcagcgg ttcctggcgc
     6721 aaagccaatc gagacttgcc ccgataatga ctgaccaacg ttatgcaccg ccagttgggc
     6781 ctgctcacac tgacgtaaaa tggcccgtgc atgggtatag agaatttttc cggcgtctgt
     6841 tggcgtaacg ccccgttttg tgcgaatcaa aagctgttga tttaactcac cttccagtat
     6901 ggcaacctgt tggctgagcg ctggttgtgc gatatgcaat acttcagcag cctgggtcag
     6961 gctaccaata tctacaattt ttacgaagta tttcaggcgt ctgaagttca tgttgcctcc
     7021 ggtttttaag aatcggccca agtgccgcca ttacttacaa ccagtatgca agatgcctgc
     7081 cagttctatt ttggcgttga tgcgcaagct aaccaactgt caaataagag attatgatag
     7141 attcgtcatt ggcccttttt atcagcagtc gcattcacct gccctataaa aggagggtat
     7201 gcaccacgat ggttcattac ccaataagat tgaaagctca ccactttgtt gaaattggca
     7261 gcaaacaaac aaaaaaaatg catttcaccc tttgacatca ccatgcactg ccattaatat
     7321 gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg
     7381 tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg ctttcgagca
     7441 ggctttttac tttctaatta ccaacgctct taaaacatct gtcttgaacc agaactaatt
     7501 tgcacaggca ttcccgatcg atgttgcaac gcagcatttg cgcgatttac atcaacttct
     7561 tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt tcggtcagac
     7621 tcatattctg cacgattgcg cgacagcggc tcatgaactt ccagccactt tgaaccatcc
     7681 ggttccgttg tgtatttcac tggctgatca ataatttgta cacgagtgcc tacaggaaca
     7741 ttatcaaaca gatatttgat atcgtcatta cgcagacgaa tacagccctg acttacacgg
     7801 agcccaatac caaaattggc attggtaccg tggatggcat acaacctgcc aatataaatc
     7861 gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa atgcgggcaa actctcccct
     7921 cgtttcgcat attcgcgccg gatgttcggc gttggcgtcc aggttggtgc ttcttgttta
     7981 cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc gataccaata
     8041 ggaaagactt ccactgtatt actgtctggc ggatagtaat aaagacgcat ttcagcgacg
     8101 ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg aatggtgagt
     8161 tgtgagccag gcttcggcaa aaaaacatca gcgcccggat tcgcttccag catattactt
     8221 aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gagtattgtg atcaggaaca
     8281 gttacagtaa gcgactgccc cactaaacgg ctaccctctg gaggtaatgg ataagttacc
     8341 gccaggctat tttggctggc aaaaagcaga gcaaatgagc aaagaatatt tacacgacgc
     8401 atcatgtccc tttcctaagt cgcgaagact atccgataag tatagctttt atcagacttt
     8461 tcgtttttag ctgttcaaat cagaagtcgt attccccggt agaacaatat tactggcagc
     8521 aagttcgccc atgttgttgt atatcgcaca agcagcttcg atgatggaca tcgccagagc
     8581 tgcaccactc ccctcaccta aacgcatatc catattgaga taaggctcca gccccaaatg
     8641 cgagagcgct atacgcgcgc ctttttctgc cgacaagtga gaaggaatga gatacggttt
     8701 gattgcagga gacatctggc aggctgcgag cgcagcagca taagaaagaa atccatccag
     8761 caacacgggt aaaccgcagg aagcagcacc taacatcact ccagctattc cgaccaaatc
     8821 aaatccaccc acttttgcca ggacatcaac accatcctga ggatttggtt gattcaacgt
     8881 aatcgcccga cgcacaacat caattttatt agccagttta tctgtcggca ggtttgcgcc
     8941 aatcccaacc acttcttcag gatcccggcc agtgattgtg ctgactattg ccgctgccgg
     9001 tgtcgtgttt gccatcccca gttcacctac accaaacagc gtgacaccgt ttttcgccag
     9061 ctcccgcgta taacatatga cgtccaaaag caacttttca gcctgacggc gactcattgc
     9121 cggagctgaa gcaatattgc cgctacctcg tgcgacacgc atgttgataa gcccggggat
     9181 aggctcagca gtatcaatac caacatcaat tacgtggacg ttagcgcccg cttgtgctgc
     9241 cagcacacac acgccggttg ttccacgggt catattttca gcctgtatgg ctgtcacttc
     9301 ttttggagaa atagcgaccc cttcctccca gacgccgtga tcggcacaca taaccagtac
     9361 cgcttttttg cccacatgcg gtatgccatt cacccccggc attcctgcca gttgtatggc
     9421 aagcgcctcc agctttccta ggctaccaac aggtttgaat aacccgtcaa catgccgttg
     9481 tgcacgcgac atagcggcag aattgatggc tgggatcgta ttcagtaaat cggcaattat
     9541 ttgcatgtca cgtttcttta tagcagtgcc agcaggaaga ccagttcacc aagctctatg
     9601 gctgctccca gcgtatcgcc cgtttgcccg cccagcgtac gtttgagaag ctgaccgagg
     9661 atgaaaatcg ccaccatcgt caccaccata gcagccacac catgcatacc cagcaataat
     9721 acggcagcaa aaatagcggc taagccgagt gtgacgcagg tttgtcgccc atcaattttg
     9781 ccaatgaaca cattgccaag cccttcttcc cgcgcataac gatgacgata catcaataat
     9841 gcggcagtcc cgcggctgac cgcacatgct gccgccagcg atgcaaggat cggttcgcca
     9901 cgcagagcca actcgcttaa caccagaatc tttgccagta ccacaaaaat caatgccaga
     9961 ccgccgtggg tgcctaaacg gctatcacgc atgatttcca acatgcgatc gcgactacgt
    10021 gcagaaaata cgccatcaca ggtatcagcg aggccgtcca gatggaattc cccggtcatc
    10081 agcgccagca caagtacgct aaacagtgcc gccagtggtg cgccacacca tgcctgcagc
    10141 accatgaaga ccagcccgct aatcgcgcca agcaataatc caatcaaagg aaaagtaata
    10201 ataccgcgag aataatgttc gaaatccagt ccctgggacc agcgacgcgg tacaggcaga
    10261 cgcgtaataa atgagagcat cgcccaaaat aatttgctca tttgattttt actccaatac
    10321 ccgaaaccac cagccatact tcatttgccg cagcggccaa ctgctgattt acccgcccgg
    10381 caatatcacg aaaatgtcgt gccagacgac tctccggcac aatccccatt cccacttcgt
    10441 tagtcactaa tacaaccttt gcggggcaac gttggcaggc agcaatcaac gactgaatct
    10501 cagcattaat cgcctgttcc atcgcctgat aatcccattc atcagggtct ttatcgccgc
    10561 cataatcaaa caacagatta gtcaccattg tggtaacgca ttccagcaac acagcctcat
    10621 tcgggttaat gtctgcatga attaattcat caagatgttg ccagcgctcc actgtgcgcc
    10681 agtgctccgg gcggctttgc cgatgatgtt ctatccgtgc agccatctca tcatcaagga
    10741 tttgcgaggt agcgatatac agaacctgtg aa
//
LOCUS       DAJTPG010000121        10555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTPG010000121 DAJTPG010000000
VERSION     DAJTPG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            598..795
                     /locus_tag="OO230_003407"
     CDS             598..795
                     /locus_tag="OO230_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693040.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(745..1938)
                     /locus_tag="OO230_003408"
     CDS             complement(745..1938)
                     /locus_tag="OO230_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5693041.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2074..3798
                     /gene="iucA"
                     /locus_tag="OO230_003409"
     CDS             2074..3798
                     /gene="iucA"
                     /locus_tag="OO230_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5693042.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3799..4746
                     /gene="iucB"
                     /locus_tag="OO230_003410"
     CDS             3799..4746
                     /gene="iucB"
                     /locus_tag="OO230_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5693043.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4746..6488
                     /gene="iucC"
                     /locus_tag="OO230_003411"
     CDS             4746..6488
                     /gene="iucC"
                     /locus_tag="OO230_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5693044.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6485..7822
                     /gene="iucD"
                     /locus_tag="OO230_003412"
     CDS             6485..7822
                     /gene="iucD"
                     /locus_tag="OO230_003412"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5693045.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7828..10023
                     /gene="iutA"
                     /locus_tag="OO230_003413"
     CDS             7828..10023
                     /gene="iutA"
                     /locus_tag="OO230_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5693046.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2587 a         2679 c         2782 g         2507 t
ORIGIN      
        1 tggagtgaaa caaaggcagc ttatcgttgt ttttcaaacg ataaagtctc tgctaataaa
       61 atcatgactc ctcacaaaga aaatatcact gaaagagctc agcagtttaa aagagtttta
      121 gttttacagg atacaacaga acttaattat tctggtcaga aagaaaaaca aggagttggt
      181 cccaaaaaac ataaagatga aagaaactta tttcttcatc ctcaactcgt catcagtgag
      241 tcaggtgttt gcctcggtgt ttatgatgac tatcagtggt tcagggatga attaaaaacc
      301 agtaaaaata cccgacaaga aatctgcaat gatttacttc ataaaaaaca tgtcagtgaa
      361 aaagaaacct ggcgttgggt tgaaggatat aataaagcaa cagaactggc tcgccaatgc
      421 cctgataccc atgtattgtc aatttctgat cgtgaaggcg atttttatga cctgtttgaa
      481 cgcgcagcgc agactcccgg aataaaagct gactggcttg tcagaatgaa atttaataac
      541 agagcaacgc tgaatattaa tggtaaacgg gatcataggt tgttgcacga aagaatcatg
      601 gagattacac cgcagcaact ggttgaattc actatacctg atgggcgggg gcagcaagcc
      661 agaacactgc ctgacatgct ttcatcagac gcaggtcctc tctcatatcc tctgatttac
      721 tccagtaagg tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga
      781 cacaatggta aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc
      841 tgttttcgat accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt
      901 agcgagccag cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc
      961 gggtgttgcc agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag
     1021 ctgttcggag agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag
     1081 ggccaccgga gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac
     1141 tgcggacaga cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtagcgaa
     1201 ctggcgagta agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa
     1261 tattgcgcaa agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg
     1321 tgtgggctgt ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggggc
     1381 tgccatcagc agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag
     1441 cggcccgaat agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg
     1501 attcccttct ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac
     1561 ggtcagcccg taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat
     1621 ggcgctggta gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc
     1681 tcccagagcc cacaacacca caggtttact gccagtacgg gaaatttgct ttccccacca
     1741 cggggacgat ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag
     1801 actcagattt gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat
     1861 accgagaagc ccggcactga acgcaagtgg ccagcaggac agtggttttt ggggcgtatc
     1921 ttcgatccct ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa
     1981 aattaattta tctgacaata acatttattg ttgataatga gaatcattat tgacataatt
     2041 gttattattt tactgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc
     2101 acagatgtgg ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa
     2161 ctgacagaat acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat
     2221 ttcagagtgg cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg
     2281 gtcacggcat caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat
     2341 aagctacgcc atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg
     2401 ctggaaagcc atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg
     2461 cgtgaaaaag cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc
     2521 caccctgcgc ctaagtctca tgaaccgttt aaccggcggg aggctgaacg ctacctgcct
     2581 gatatggcac cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt
     2641 gaaagtttgc atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct
     2701 cagttactca acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg
     2761 gaatatcttt tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac
     2821 ttaggtgagg ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc
     2881 accagccgcg atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact
     2941 ctgtccgtga aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc
     3001 tggcagatgc tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc
     3061 gggctgctcg atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat
     3121 ctgctggtgg atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca
     3181 cctgatggcg gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt
     3241 atcactgtgc aacaggccgc ccatgcatgg gttgatgcat actgtcagca agtgctaaag
     3301 ccgctgttta cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt
     3361 cttgtccaga tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc
     3421 gcttttatgc ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat
     3481 attttcaccc atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact
     3541 tttgctgtga ccgctgcgct gggggctgcc gggctggaca gcgaatcgaa tctgatggct
     3601 cgtgtacgag catcgctggc tgaagtgcgt gatcaggtga ctcataaaac ctgtctcaac
     3661 tacgtgctgg aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat
     3721 cataacgaga acaccattgt tgacccttcg gtgatctatt tcgatttcgc taacccgctg
     3781 caggctcagg aggtctgaat gtctgaggca aacattattc acagcagata tggactgcgc
     3841 tgtgaaaaac tcgacaagcc cctgaatctt ggctggggac tggacaatag cgcggtgttg
     3901 cactgtcccg gggagctgcc gacagggtgg ctgtgcgatg cgctggatca gatatttatc
     3961 gccgcaccac aactgtcagc agtggctctc ccctgggccg aatggcgtga ggagccacag
     4021 gcgctgacgc tttttgggca ggtaaaaagc gacattatcc atcgtactgc cttctggcag
     4081 ttaccgttat ggttgagttc accggcaaac cgggcctccg gtgaaatggt ttttgatgca
     4141 gagcgtgaga tttatttccc gcagcgccca ccccgtccac agggtgaggt ttaccgccgt
     4201 tacgatccac gtattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca
     4261 gaacgtttca cccgctggat gaatgatccg cgcgttgagt atttctggga gcaaagtggc
     4321 tcactggagg tacagaccgc ctatctggag cgccagctca ccggtaaaca tgcattcccg
     4381 ctgattgggt gcttcgacga tcggccattc agctatttcg aaatctactg ggcggcggaa
     4441 gaccgcattg gtcgtcacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt
     4501 ggtgaacagc aatggcgcgg ggcccactat gtgcaaagct ggctgcgcgg gctgacacat
     4561 tacctgctgc tggatgagcc ccgtacgcag cgcacagtac tggagccacg caccgataac
     4621 cagcgcctgt tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc
     4681 ccacataagc gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt
     4741 ctgtaatgaa tcacaaggac tgggattttg ttaaccgtcg gctggtggca aaaatgttgt
     4801 ctgagatgga gtatgagcag gttttccacg cggaatctca gggcgatgac cactactgca
     4861 ttaacctgcc gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctgt
     4921 ggattgatgc ccaaacgttg cgctgcacgg acgaaccagt actggcgcag acgctgctga
     4981 tgcagctaaa gccggtgctg tcaatgagtg atgccactgt tgcagagcat atgcaggatt
     5041 tgtatgccac gctactgggc gacctgcaac tgctgaaggc ccgtcgcgga ctgagtgcca
     5101 gtgacctgat taatcttgat gccgatcgtc tgcaatgcct gctgagcggt catcctaaat
     5161 tcgtttttaa taaaggtcgc cgtggctggg gcaaagaggc gctggaacga tatgcgccag
     5221 agtataccaa caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc
     5281 gctgcgataa cgatctggat attcagcagt tgttgactgc cgcaatggat ccgcaggagt
     5341 ttacacgctt cagtcaggtc tggcaggaaa acgggctgga tcacaactgg ctgccgctgc
     5401 cggtacatcc gtggcagtgg cagcaaaaaa tcgccacaga cttcatcgct gattttgccg
     5461 aaggcaggat ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc
     5521 gtaccctgac caacgccagc cgccggggag ggctggatat caagctgccg ctgaccatct
     5581 acaatacctc atgctaccgg gggatccctg gcagatatat cgctgccgga ccactggctt
     5641 cacgctggct gcaacaggtt tttgcgaccg acgccaccct ggtgcaaagc ggcgcagtga
     5701 tccttggtga accggctgca ggctatgtgt cccatgaagg ctatgccgcg cttgcccggg
     5761 ctccctatcg ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc
     5821 tgaaaccgga tgaaagtcct gttctgatgg caacactgat ggagtgcgac gaaaacaatc
     5881 agccgctggc aggcgcatat atcgaccgct ccggactgga cgctgaaacc tggcttacgc
     5941 aattgttccg ggtggttgtg gttccgctgt atcacctgct ttgccgctac ggtgtcgcgc
     6001 ttattgcaca cggacaaaat ataacgctcg ccatgaaaga gggggtacca cagcgtgttc
     6061 tgctgaaaga cttccagggc gatatgcggc tggtgaaaga agcgtttccc gaaatggact
     6121 ctttgcctca ggaggtgcgt gatgtcacat cccgtctgag tgcggactac ttaatccatg
     6181 atttgcagac gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc
     6241 ttggcgtacc tgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga
     6301 acaaacatcc acaaatggca gagcgttttg cgcttttctc actcttcagg ccacaaatca
     6361 ttcgcgtggt gctgaacccg gtaaaactga cctggccgga cctggatggc ggcagtcgca
     6421 tgctgccgaa ttaccttgag aatctgcaaa atccgctgtg gctggtaact caggaatatg
     6481 aatcatgaaa aaaagtgtcg attttattgg tgtcgggaca gggccattta atctcagcat
     6541 tgctgcattg tcacatcaga tcgaagaact gaactgtctc ttctttgacg aacatcctca
     6601 tttttcctgg catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa
     6661 agatctggtc agtgcagttg cacctacaaa tccctacagc tttgttaact atctggtgaa
     6721 gcacaaaaag ttctatcgct tccttacaag caggctacgt acagtatccc gtgaagagtt
     6781 ttctgactat ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt
     6841 tgaaaatatt gatttcgaca aaaaaagtcg attgtttctg gttcagacca gtcggggaga
     6901 atattttgcc cacaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt
     6961 gaagcatgtg acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct
     7021 gagtggaaaa cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa
     7081 tgcattacgc ggggaatggg gagaagcggc ggaaataaac tgggtctcac ggcgtaataa
     7141 ttttaacgca ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc
     7201 aggcttctcc ggactgaagg aagatattcg ccatcagtta ctggatgagc agaaaatgac
     7261 atcggatggc atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt
     7321 tgaagttctg agaaaaccaa gaaatatccg tctgctaccc agtcgctcgg taacaactct
     7381 ggaaagtagt ggtccgggct ggaggttatt gatggaacat catctggatc agggcagaga
     7441 gagcctggaa agtgatgtgg tgattttcgc cacaggttac cgttctgcat tgccacaaat
     7501 acttccctca ctgatgccct tgatcaccat gcacgataag aacaccttta aagtgcgtga
     7561 tgacttcact ctggaatgga gtggcccgaa agaaaataac atctttgcgg tcaacgccag
     7621 catgcaaact catggcatcg ccgaacccca gctcagcctg atggcctggc gatctgcacg
     7681 tattcttaat cgcgtactgg ggcgtgattt attcgatctc agtatgccgc ccgcactgat
     7741 tcagtggcgc agcggcagcc ggaaaaaacc acagccggag gctgcttctt taactcacta
     7801 tacagcaaat attcaggaat aacgatgatg cgcaaaaagt atatgccccg ggctcttggt
     7861 ccgctgcttc ttgtcgtgct gtcaccagct gtcgcccagc aaaacgatga taatgagatc
     7921 atagtgtctg ccagccgcag caatcgaact gtagcggaga tggcgcaaac cacctgggtt
     7981 atcgaaaatg ccgaactgga gcagcagatt cagggcggta aagagctgaa agacgcactg
     8041 gctcagttaa tccccggcct tgatgtcagc agccagagtc gaaccaacta cggtatgaac
     8101 atgcgtggcc gcccgctggt tgtcctgatt gacggtgtgc gcctcaactc ttcacgttcc
     8161 gacagccgac aactggactc tgtcgatcct tttaatatcg accatattga agtgatctcc
     8221 ggcgcgacgg ccctgtacgg tggcgggagt accggagggt tgatcaacat cgtgaccaaa
     8281 aaaggtcagc cggaaaccat gatggagttt gaggctggca caaaaagtgg ctttaacagc
     8341 agtaaagatc acgatgagcg cattgccggt gctgtctccg gcggaaatga ccatatctcc
     8401 ggacgtcttt ccgtggcata tcagaaattt ggcggctggt ttgacggtaa cggcgatgcc
     8461 accctgcttg ataacaccca gaccggcctg cagcactcca atcggctgga catcatggga
     8521 accggtacgc tgaacatcga tgaatcccgg cagcttcaac tgataacgca gtactataaa
     8581 agtcagggag acgacaatta cgggcttaat ctcgggaaag gcttttccgc catcagcggg
     8641 agcagcacac catacgtcag taaggggctg aattctgacc gcattcccgg cactgagcgg
     8701 catttgatca gcctgcagta ctctgacagt gatttcctgg gacaggaact ggtcggtcag
     8761 gtttactacc gcgatgagtc gttgcggtac tacccgttcc cgacggtaaa tgcgaataaa
     8821 caggcgacgg ctttctcctc gtcacagcag gataccgacc agtacggcat gaaactgact
     8881 ctgaacagcc aactgatgga cggctggcaa atcacctggg ggctggatgc tgagcatgag
     8941 cgctttacct ccaaccagat gttcttcgat ctggctcagg caagtgcttc cggagggctg
     9001 aacaaccata agatttacac caccgggcgc tatccgtcat atgacatcac caatctggcg
     9061 gccttcctgc aatccagcta tgacattaat gatattttta ccgttagcgg tggcgtacgc
     9121 tatcagtata ctgagaacag ggtagatgat ttcatcgact acacgcagca acagaagatt
     9181 gctaccggga aggcgatatc tgccgacgcc attcctggtg gttcggtaga ttacgataac
     9241 tttctgttca atgctggtct gctgatgcac atcaccgaac gtcagcaggc atggttcaat
     9301 ttttcccagg gggtggcatt gccggatccg gggaaatatt atggtcgcgg catctatggt
     9361 gcagcagtga acggccatct tcccctgaca aagagcgtga acgtcagcga cagtaagctg
     9421 gaaggcgtga aagtcgattc ttatgaactg ggctggcgct ttatcggtga caacctgcgg
     9481 actcaaatcg cggcatatta ctcgctttcc aataagagcg tggaaaggaa taaagatctg
     9541 accatcagtg tgaaggacga caggcgccgt atttacggcg tggaaggtgc ggtggactac
     9601 ctgatcccgg atactgactg gagtaccggt gtgaacttca atgtgctgaa aaccgagtcg
     9661 aaagtgaacg gtcaatggca aaaatatgac gtgaaggaat caagtccatc gaaagcgaca
     9721 gcttacatta actgggcgcc ggaaccgtgg agtctgcgtg tacagagcac cacttctttc
     9781 gacgtaagcg atgcagaggg taacgatatt aatggttaca ctaccgtcga ttttatcagt
     9841 agttggcagc ttccggtggg aacactcagc ttcagcgttg agaacctctt cgaccgtgac
     9901 tataccactg tctggggaca gcgtgcacct ctgtactaca gcccgggtta cggccctgct
     9961 tcactgtacg actacaaagg ccggggccga acctttggtc tgaactactc agtgctgttc
    10021 tgacggggat gcttttgcaa caagggtatg ctgtaaacaa catggtcctt ggtggccatg
    10081 ttgtttacag ccagtataat atacagcctt aacaagatat tccgtctccg gaatgggctg
    10141 ttcaccactg aataacatta tgtctgtttt atggatcttt gactgaaatt tatcgcatat
    10201 cctcatttca ttgtccgtct gaaaactaac cttacaagta agcgtcgtct cagcaccgtc
    10261 tggcagatcc tgaaattcct gagaggatga tctgcgccca atatcctgag ccggaacttt
    10321 gctgcaatga aggccaacga aaaatggggg agtatgttat agagtacttg ctacagggta
    10381 aaaagaggta cctgtcgctg gttctccgtt cagtttaacc gggagatttt ctcctgcgcc
    10441 tgtcataaag tccggtaatg gagattgtta atacgatggt gcacgatgca tcgtcagtgc
    10501 ccggcccgga ggatgccccg tagctacata cggatcaggg ttggcaatat cgaat
//
LOCUS       DAJTPG010000122        10294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTPG010000122 DAJTPG010000000
VERSION     DAJTPG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..401
                     /locus_tag="OO230_003414"
                     /pseudo
     CDS             1..401
                     /locus_tag="OO230_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="OO230_003415"
     CDS             484..741
                     /locus_tag="OO230_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693047.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO230_003416"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="OO230_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5693048.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO230_003417"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="OO230_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5693049.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO230_003418"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="OO230_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5693050.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO230_003419"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="OO230_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5693051.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO230_003420"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="OO230_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5693052.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO230_003421"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="OO230_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5693053.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO230_003422"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="OO230_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5693054.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9142..9324
                     /locus_tag="OO230_003423"
                     /pseudo
     CDS             9142..9324
                     /locus_tag="OO230_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9318..9422
                     /locus_tag="OO230_003424"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="OO230_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="OO230_003425"
     CDS             9762..10019
                     /locus_tag="OO230_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5693055.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2401 a         2735 c         2822 g         2336 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaacgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agca
//
LOCUS       DAJTPG010000123        10202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTPG010000123 DAJTPG010000000
VERSION     DAJTPG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            415..546
                     /locus_tag="OO230_003426"
     CDS             415..546
                     /locus_tag="OO230_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5693056.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            806..1801
                     /gene="pgl"
                     /locus_tag="OO230_003427"
     CDS             806..1801
                     /gene="pgl"
                     /locus_tag="OO230_003427"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5693057.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1842..2795)
                     /locus_tag="OO230_003428"
                     /pseudo
     CDS             complement(1842..2795)
                     /locus_tag="OO230_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2979..4031
                     /locus_tag="OO230_003429"
     CDS             2979..4031
                     /locus_tag="OO230_003429"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5693058.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4107..5540
                     /locus_tag="OO230_003430"
     CDS             4107..5540
                     /locus_tag="OO230_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5693059.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5723..7903
                     /locus_tag="OO230_003431"
     CDS             5723..7903
                     /locus_tag="OO230_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5693060.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8044..9327)
                     /locus_tag="OO230_003432"
     CDS             complement(8044..9327)
                     /locus_tag="OO230_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5693061.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9462..10154)
                     /locus_tag="OO230_003433"
     CDS             complement(9462..10154)
                     /locus_tag="OO230_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5693062.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRA
                     AKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         2504 a         2365 c         2556 g         2777 t
ORIGIN      
        1 gccataaggg aatgagtgtt atatcatgct ttctacccta gtaagtatat ttgctttttt
       61 cgtatatttg gttcatcttt ttttgatgtt ttacatattc acaatgcaga ggatttatac
      121 cagaggacat tagtcttgag taagatacta caacatctcc aactatttta gccagcctga
      181 ctacttgact tggtaatatt tattgctaat aatcatgtat aaaataagct tatatacata
      241 gtaacttcag caaaggattt agtaatgatc aaaatcatat ctcaatgtac tgtttttttc
      301 tttaaaaaaa cagtacattc tattttataa tgactgtgta ttcccatgga aggagatcac
      361 ccaattctcc cccccgactt ttgacgcagt gtcttgttaa cagggggatt gaccatgctt
      421 attggctatg tacgcgtatc aacaaatgac cagaataccg atctacaacg taatgcgttt
      481 atttgtgccg gaagtgagtt gattgttgcg cgaatgggaa gttggccaga agttgttatg
      541 gcatagtgtt tacggtcgtt gatacaggta aacaaaagcg gggagagtca tactccctga
      601 atagtcgcga aaatatctgt ggcattgtca aattcgtgtc ggtgactaat attttagtaa
      661 tatgccattc gaaatggagt gctaacttat tgatttaatt gatgtgcgat attgtatttt
      721 atcgtgtttt gttgtctgtt ttttatccag taagtgttgt tatatgtatg aaagataagg
      781 gatttatcat ttttgaggtg tgttcatgaa gcaaacagtt tatatcgcca gccctgagag
      841 ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt
      901 cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt
      961 tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatt gccccggacg atggcgcact
     1021 gacctttgcc gcagagtctg tgctgccggg tagtccgacg catatttcca ccgatcacca
     1081 ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga
     1141 agatggcctg ccagtgggcg tcgtcgatgt agtcgagggg ctggacggtt gccattccgc
     1201 caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg
     1261 cctgtttacg gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac
     1321 cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat atgcgtattg
     1381 cgtcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc acggcaatat
     1441 cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc
     1501 tgatattcat atcaccccgg atggtcgcca tttatacgcc tgcgaccgta ccgccagtct
     1561 gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag gcttccagcc
     1621 aacggaaacc cagccgcgcg gcttcaatgt tgattacagc ggcaagtatc tgattgccgc
     1681 tgggcaaaaa tctcaccaca tctcggtata cgaaattgtt ggcgagcagg ggctactgca
     1741 tgaaaaaggc cgctatgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta
     1801 accgctgatt taaccggcgc agtctctcct gcgccggtgt attaacttat ctcctgtaac
     1861 gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa
     1921 ttaatcagca aggaaaaaaa gcgttcagct gccggagaaa gcacggcatc tttgcggcga
     1981 ataatcccca atgtccgacg tataacgggt tccaccagtg ggataccaat aatcgaagaa
     2041 tagggcgcat ggggcattgc cagaccagga agcgccgaaa tccccagtcc tgcctccact
     2101 agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg
     2161 tcggctagct gttgttctat caacaatcgg ttgccgctgg aggaacgtac gccaatcatt
     2221 ttgtaaccca ctaactcctg ccattctaca agctactttt tggccaatgg atggtcacgc
     2281 cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg
     2341 acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc catgcaatta
     2401 ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg
     2461 attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaatgt caccatacca
     2521 cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat
     2581 tcccgcgcct ccgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta
     2641 cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg cgttaatgca
     2701 ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg
     2761 acaaatgcct tcatacttga taattcatgc ttcattatta ctccggaaaa tggaagcgac
     2821 gattttgggg ggctggtcgt taaaaatttt aactgcattt agccaactta aattaatgaa
     2881 aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgtcctta atgtaaccat
     2941 atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga
     3001 tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc
     3061 aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg
     3121 acggtattgg cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg
     3181 atccgcgtgc tgatgtcgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg
     3241 ataccacgcc taactgcggc aatatgctgt ctggcgtagg ggcattcgcc attgaaaatg
     3301 gtttgattgc agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta
     3361 cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca
     3421 gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg
     3481 gaacgaaaac tggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc
     3541 cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa
     3601 aaacaggtta tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg
     3661 aatctatccg gctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta
     3721 tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt
     3781 ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta
     3841 gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga tatggcaata
     3901 tcaatattga acaccccagt ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg
     3961 ccacgacgtt acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt
     4021 atcttccctg aaaaaattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga
     4081 gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga
     4141 tattagcgat cccatgtatt attggtttta tgccagctcc ggcaggatta agcgaactgg
     4201 cgtgggtgct ttttggtatt tacctggcgg ccattgtggg tctggttatc aagcctttcc
     4261 cggaacctgt cgtactgtta attgccgttg ctgcctcaat ggtggtggtc ggtaacttat
     4321 ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc
     4381 tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaactta
     4441 ttgcctatct gctgattggt aaaatcggta acaccatgct gggtctgggt tacgttacgg
     4501 tattcctcga tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca
     4561 ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc
     4621 cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct
     4681 atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc
     4741 tgcacctgca aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta
     4801 tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg
     4861 ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa
     4921 aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg
     4981 gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg
     5041 ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg
     5101 gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag
     5161 tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt
     5221 tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt
     5281 tacctgtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg
     5341 cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc
     5401 cggtcatctt tggcgtgggt tacaacgata ttaaatcctg gtggttggtc ggtgcggtac
     5461 tgacgatatt aaccttcttg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga
     5521 tcggctggaa catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc
     5581 gttcgctcac cccagtcact tacttatgta agctcctggg gattcactca cttgtcgcct
     5641 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
     5701 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
     5761 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
     5821 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
     5881 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
     5941 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
     6001 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
     6061 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
     6121 tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg
     6181 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
     6241 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
     6301 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
     6361 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
     6421 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
     6481 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gtcaggatta
     6541 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
     6601 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     6661 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     6721 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     6781 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     6841 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
     6901 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
     6961 cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa
     7021 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     7081 agccaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
     7141 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     7201 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     7261 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     7321 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga
     7381 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg
     7441 gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca
     7501 tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg
     7561 ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc
     7621 cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct
     7681 gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa
     7741 agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc
     7801 ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa
     7861 ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg
     7921 taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa
     7981 atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag
     8041 ctcttacttc ttctcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca
     8101 catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct
     8161 gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt
     8221 gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc
     8281 aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt
     8341 tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc
     8401 ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg
     8461 accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg
     8521 acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg
     8581 accgagaatg ttaacgttgt taatctgtac ttggtcaccg tcagtacgca gtgctaccgc
     8641 cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca gattttgcag
     8701 ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct
     8761 gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc
     8821 gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc
     8881 aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc
     8941 agggacatac accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt
     9001 ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg
     9061 agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt
     9121 ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc
     9181 ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg
     9241 ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc
     9301 cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa
     9361 tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat
     9421 ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg
     9481 tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg
     9541 agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg
     9601 cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg
     9661 cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt
     9721 atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg
     9781 agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg
     9841 acataaagat atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt
     9901 tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct
     9961 gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt
    10021 gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg
    10081 aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc ctcgtctatg
    10141 tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc
    10201 ag
//
LOCUS       DAJTPG010000124        10044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTPG010000124 DAJTPG010000000
VERSION     DAJTPG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            37..225
                     /locus_tag="OO230_003434"
     CDS             37..225
                     /locus_tag="OO230_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5693063.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            200..430
                     /gene="ymcE"
                     /locus_tag="OO230_003435"
     CDS             200..430
                     /gene="ymcE"
                     /locus_tag="OO230_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5693064.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            420..593
                     /locus_tag="OO230_003436"
     CDS             420..593
                     /locus_tag="OO230_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5693065.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(642..1715)
                     /locus_tag="OO230_003437"
     CDS             complement(642..1715)
                     /locus_tag="OO230_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5693066.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1817..4528)
                     /gene="torS"
                     /locus_tag="OO230_003438"
                     /pseudo
     CDS             complement(1817..4528)
                     /gene="torS"
                     /locus_tag="OO230_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4611..5639
                     /gene="torT"
                     /locus_tag="OO230_003439"
     CDS             4611..5639
                     /gene="torT"
                     /locus_tag="OO230_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5693067.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5612..6304)
                     /gene="torR"
                     /locus_tag="OO230_003440"
     CDS             complement(5612..6304)
                     /gene="torR"
                     /locus_tag="OO230_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5693068.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6391..7563
                     /gene="torC"
                     /locus_tag="OO230_003441"
     CDS             6391..7563
                     /gene="torC"
                     /locus_tag="OO230_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5693069.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7563..10044
                     /gene="torA"
                     /locus_tag="OO230_003442"
                     /pseudo
     CDS             7563..10044
                     /gene="torA"
                     /locus_tag="OO230_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
BASE COUNT         2417 a         2583 c         2675 g         2369 t
ORIGIN      
        1 cggcagcgaa cgttgttacg ctctaaggtt gccgttatta ctcaacatct ccatttccgc
       61 tgtccatgtt gtcatggttc acagtaccgt acatcggcat tcgatgtgac ggagcgaaac
      121 cctttgggcg ctaagtgtat tttttgtaaa tcgactatga tcacctttga taacgtcgcg
      181 ctgcaaatac gcactgacca tgcgccgctg gatttcacaa aataatatca ggctccctcg
      241 tggagccttt tttatatctg ccttattttt cttcaacgct gtatgtatag taagcgataa
      301 cctgttgatt attgaatctt tcgggaagat ggcttataac atttcttacc tgaccagggt
      361 accgggaacc aacaccttac tggcgtgttg ctgtctttca agaccagacg aggttaacag
      421 tgaatattga agagttaaaa aaacaagccg aaacggaaat cgccgacttt atcgcgcaaa
      481 aaatcgccga gctgaacaag aatacaggga aagaagtctc tgaaattcgc ttcatcgcac
      541 gagaaaaaat gaccgggctt gaaagttatg atgtcaaaat caaaataatg tgattttgtg
      601 aacatcaccc cgtgcgaggt gatgttccgc ttgttgctaa tttagtgacc aatcattggc
      661 gcttgtggaa ttaagcgtcg gtacaattcc tccggcaccg ggctttgcca tactcccgca
      721 tacattgcgt aaccaatcac cgcaaacata atccccagaa ccagtagcgt cattaaccag
      781 ccagacaacg caaaggcttt tttatttgct gcaggttttt gcagtgaaaa ggtcaatgtt
      841 gaggctaccg gacatgactc tacgcaagtc atacagccgg tacattccac tgttcgtacc
      901 tgaattaatt tatctaccgg gatccgtgat gggcactttt ttgcgcattt gccacaatcg
      961 atacaacttt cggcattgcg acgaatctta aacggcgaca atagcgaaac cacgcccatc
     1021 agcgcgccat atgggcaaag ataacgacac caggcatggc gaataaacag gctggcaatc
     1081 agcaaaacgg tcacgctgat taatgtcgcg gtccccatat gacgaaagaa atcgagcatt
     1141 ttaacgtcca tcaccacgct atagggcgac aacataaaat agtgaatcgc ctgagcgggc
     1201 atcaataacg cgatatagag aaataaactc aacagcaaat acttcacgcc gcgcagagga
     1261 atatccagcc agcggggaag gacacattgc cgaccaaaca gtttgttacc gagatcgccg
     1321 attaattcag aaagcgtacc aaccgggcat aaccatgagc aaaaggcctt tttgagtaat
     1381 agactgatga cgataaaagc gaccaataac agcatcgcgg cggcgtggac ggacggtaac
     1441 tgacctgttg caaggctata tttcagattc atcagaccgg caatcggtag ccagccttcg
     1501 atacctcccg gtctggcgac aaatgtcgta ctacctgccg tttcgtaata gcgcacccaa
     1561 taccagaacg tgatggcaat ataaatattc attgccaata gtaagaattg cgtcgcttta
     1621 cgccaggtcg tggcattgcg ccagtcattc cacggtaatt tgccgcccgt cgtgcctggc
     1681 cgccgctgcc agcgggttct ttttttctct gccatgattt tgccagtccg ttaagttgca
     1741 taccaaatgc cactattcta gttattctta actggctgat attgattcaa atcgcgttca
     1801 ggtctttctt attcaatcat gcttccagag cggcaacact gcgtgtaatt tcttcgtgtg
     1861 gaaggggggc agataatggc tgctgctcca gttgtgcgca tagctgactg gcgctacgca
     1921 ttcccagact cgagcaactg ctttttagct gatgcgcggc acgctttatt ttctcgctgt
     1981 cctggctggc gcgggcaatg tcgatttcat cgagaagcgg cagggcatgt tgtttaaata
     2041 atgccagcca ttcgtggatc ttctccgtcc ccattaactg agcgtcttca ttgagttgcg
     2101 agacatccag tggtagatcg ttattggctt gcagttggag atagtgcgcc agtaactgac
     2161 cgagcacttc acgcggcacc ggtttaggga taatcccgcg aaatagtgaa ctggtacgct
     2221 ggcgcagcgt ttcgtcaatg acatgggcgc taaagccaat caaaaccagc gacggatatt
     2281 gctgtgccag ttgccgggca agggtaatgc cgtcgatatc cggcagatca aaatccacca
     2341 gtgcggcagc aaacggttcg ctattttgca gtgtctctaa agcctgcgcg gcattgccaa
     2401 tagcaacaac ctgcgcacca ctggtgttca gcatctcaac ggtaattcgc tgggttagcg
     2461 ggttatcttc aattaacagc aaacgtaaac cgtcaagacg caccgcctga ttggctgttt
     2521 ttggcacggg tgcagtggca acacgtaacg gcaagcgtaa acaaaaacag cttccaacct
     2581 ccggcgtgct ggtggcgctc agttcgccgc ccatcgcctg ggccagacgg ctactgatag
     2641 tcagccccag cccggtgccg ccgcgtttgc cgcttacctg cacaaatggc tggaagattt
     2701 ctgccagttt cgcgggatca ataccgcagc cgctgtcttc cacttcgacc agccattgct
     2761 cgccatcagt gcgactacgc aggataatgt gcccttcgtc agtaaaacgc agggcattgc
     2821 tcaataggtt ggtaatgacc tgacgaatac gtcgtggatc gcccattaac gcgctcggta
     2881 catcatcggc aattgccgtt gccaggcgaa tcgggcgacc tttcacccgt ccgctcatta
     2941 attgcagggt actttccagc agcgggcgcg gttcaaaggg ctcatcgctg actgaaacat
     3001 ttttgccacc tgcttcgata gccgaataat cgagaatatc gttgaggatg gtcagcaacg
     3061 attcgccaga gtcggtaatc gcctgcaaat catcacgctg ggcgttaagt gcggggttat
     3121 ctgccagcag ttgagcagtg ccgagaatac cgtacagcgg tgtgcggatc tcatggctca
     3181 tcgccgtcag aaacgccgat tttgcctggc tggctttttc tgcttccgcc cgtgcctgtc
     3241 ggtgttctat caccagttcc tgcaattcag ctgtacgcgc tttgacctgc gccgccagct
     3301 gttcgcggtg gcgattcagt gcatgaacac tgctgcgaaa cgcatccatc agccgcccga
     3361 tggtatccag ctcccgtacg ccagcggttt ccgggaaagg ggagtcaata tcaccgtcca
     3421 gcagccgttg cagcgcctgc gtttgttcgg caagtggacg cgtgaatgag cgataaacca
     3481 cgcgccagag gatcataatc agtgcgcaaa gtgaaaccat ccccagcaat aacaggctgt
     3541 attgcccgcg tgcactggct ttttccagat gtgccagtcc atgctgattg tgcagctcaa
     3601 tggtgtcgac cagctgactg acttcgctac taaactgcgc gaactgggcg atattatttt
     3661 gggcgagagt ttgcaggtga ttgctgattt cactgtcctg ctggaacagc gccaacagat
     3721 cgctatattg gctaacggta gttaacgttg ttgcgacctg cgcacgaact cccggatctt
     3781 caatgcgtat ttgccgacgt tgcagaattt tcaccgcatt attgagctgc ttttccagcg
     3841 ttggtgcatt tttctggatc tgctccagcc ccagattcat caccatttgc tgcacccgca
     3901 gagcgctaag gcgcagttca ttcatctggt taacatactc aagatcgata tcaatcagcc
     3961 gatcgagtgc actttcagca gcctgacgct gatcttgttc gatcaaatcg taaatcccgg
     4021 cctgggtcgc tccagcggaa gttgtcgcat tattcgcctg accttgctcc aggcgtgcga
     4081 tctcatcggc ggcagctatc tgctgactga gttgccgttg ttgctggcgt agttgcagac
     4141 gccgccccac cagttcccct tgctgacgta atgaacggga gatctcctgc tcctgttgtt
     4201 caatagcggt ggtatcaaaa ccttgttccc gtaacgcttg cagcaacgca ttaatcttca
     4261 ggctttatgc ggtgagcatt cgcccctgcg cctgccacat cttttcgtta tcggcactgg
     4321 tcaggttctg cgcggcgaaa agttcccagg cgctggcttc gctcaactgg cgcgccatat
     4381 tcatggtagg aatcaatgcc tgagtattgt ctttttccac ctggctgata aagcgcaggt
     4441 tgtaccatcc caccagggta ctggtcaggg ttaacagcgc catcagggca aagcccatcc
     4501 agagtcttcg ggtcagggtt aaattcacgg tcggtgcact taaggtgaaa aaggttgagt
     4561 cgcaaagtgg aatgcatcta gcataaagcc ttattattga tgaggctatc atgcgcgtac
     4621 tgctattttt acttctttcc cttttcatgt tgtcggcatt ttcggctgat aacctgttgc
     4681 gctggcatga tgcgcagcat ttcacggtgc aagcctctat gccgcttaaa gccaaacgcg
     4741 tatggaaact gtgcgcgctt tatcccagcc tgaaagattc atactggtta tcgttgaact
     4801 atggtatgca ggaggctgct cgccgctacg gtgtggattt aaaagtgctg gaggcaggcg
     4861 gctacagcca gttggctacc cagcaagcac aaatcgacca gtgtaaacag tggggcgcag
     4921 aggccatttt gctcggtagt agcacgacgt catttcccga cctgcaaaag caggtagcaa
     4981 atctgccggt gatcgaactg gtaaatgctc ttgatgatcc ccaggtgaaa agccgcgttg
     5041 gtgtaccctg gtttcagatg ggctatcaac ctgggcgata tttggtgcaa tgggcgcacg
     5101 gtaaaccact gaacgtgctg ctgatgcccg gacccgataa cgccgggggc agtaaggaga
     5161 tggtcgaggg ttttcgcgca gccattgccg gaagcccggt gcgtattgtt gatattgcgc
     5221 ttggtgataa cgatattgaa atccagcgca acctgttgca ggagatgctg gaacgccacc
     5281 cagaaatcga cgtcgttgcc ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc
     5341 gtaacctgaa aacgccgctt accgtggtgt cgttttatct ttcacatcag gtgtatcgcg
     5401 ggctgaagcg gggaagagtg attatggctg ccagcgatca aatggtctgg cagggggaac
     5461 tggcagttga gcaggccatc aggcaattac aggggcaatc ggtgtctgat aatgtcagcc
     5521 caccgatttt agttctgacg ccgaaaaatg ccgaccgaga acatatccgc cgctcgctgt
     5581 caccgggggg atttcgtccg gtctattttt atcagcacac atcagcggct aagaaataac
     5641 cttcaccatg ttgcgtcacc agtaaatccg cgctgagttt atgacgtaaa cgacgaatta
     5701 acacatcgac ggtgcgcagg tcagggtttt ccacccgacg cgcagaaagc atacgcagca
     5761 gacgctcacg gctgagaatt tcgcccggat tagtcacaaa tgccaccaac atttcatact
     5821 ctgcgcgggt cagtttaatc ggctcgccat cccgctccag cgtatggcgc gacacattca
     5881 ggcaataacc cgcaaagcga tagcagtagt cctgagtgtg cggttgagct tgtcgcgcga
     5941 ggtcgattcg ccagagcaga tttttcaccc gtactaccag ttcgcgcagt tccagcggtt
     6001 tggtgacgta atcgtctgcg cccatttcca gcccaacaat acggtcaatt cgatcgctgc
     6061 gtccggtaac cagaataatc cccaccgttg agcgttctcg cagggcgcgg gttaacatca
     6121 ggccattttc atcgggtaag ttgatatcca gcagaattaa atctaccggc tgattctgca
     6181 taatttcccg tagcccggca ccgctcgccg taacggaaac ggtatacccc tcctgagtga
     6241 agtaggattg taatcgcgcc tgggtaaccg gcttatcttc aacaataaca atgtgatgtg
     6301 gcatcagagg gttttactca ttctgttcat atctgttcat atttgctcat taagatcgct
     6361 tcactaaacc ataattctac aggggttatt atgcggaaac tctggaacgc gctacgccga
     6421 cccagtgctc gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg
     6481 ctgattgtat tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt
     6541 tgccacagta tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc
     6601 tccggcgtgc gagctgaatg ccatgactgt catatcccgc cggatattac aggcatggtg
     6661 aagcgcaaac tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat
     6721 acacctgaaa aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg
     6781 aaagaaaaca actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc
     6841 aggcagcatc ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc
     6901 atcgactgtc ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag
     6961 cagttcgatg agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat
     7021 attaagccaa tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct
     7081 tcggaagtga aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg
     7141 acggaaagcg ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt
     7201 gcctcgattc gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc
     7261 gacaccaata ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg
     7321 gtgaacgata tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac
     7381 gagtgccacg gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc
     7441 aacggcatga ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat
     7501 ctgcaaatga atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa
     7561 taatgaacaa taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg
     7621 gcttaaccgt cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg
     7681 cgcaagcggc gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg
     7741 gggctatccg cgcgatggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg
     7801 ataaatatcc gtcgaaaatg atcgccggat tgccggatca cgtacacaac gcggcacgta
     7861 ttcgttatcc gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc
     7921 agcgcggtga taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg
     7981 aagaactgga acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt
     8041 ggcaatcgac ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc
     8101 atggtaatag cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc
     8161 cgcgcgtagt cggttcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc
     8221 agaacagcaa aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact
     8281 ggtggtgccc ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg
     8341 ccggtgaagt tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg
     8401 ggcgcgagca tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg
     8461 cgctggcaca tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact
     8521 gtgtgggttt tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag
     8581 atgccgcatg ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc
     8641 ggcagatggc ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc
     8701 acggtgaaca gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc
     8761 tgccaggtgg tggttttggc tggcactaca acggcgcagg cacgccgggg cgtaaaggcg
     8821 ttattctgag tggtttctcc ggctctacgt cgattccgcc tgttcacgac aacagtgact
     8881 acaaaggcta cagcagcact attccgattg cccgttttat cgatgcgatc ctcgaaccgg
     8941 ggaaagtgat caactggaac ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt
     9001 ttgccggaac taacccattc catcgccatc agcagatcaa ccgcattatt gaaggctggc
     9061 gcaagctgga aacggttatc gccatagata accagtggac ctcaacctgc cgctttgccg
     9121 atatcgtgct gcctgcgacc acgcagtttg agcgtaacga tctcgaccag tacggcaacc
     9181 actccaaccg tggcattatc gccatgaaac aggtggtgcc gccgtagttc gaggcgcgca
     9241 acgactttga tattttccgc gagctgtgcc gtcgctttaa tcgtgaagaa gcctttaccg
     9301 aagggctgga cgaaatgggc tggctgaaac gcatctggca ggaaggtgta caacaaggca
     9361 aaggacgcgg cgttcatctg ccagcgtttg atgacttctg gaataacaaa gagtacgtcg
     9421 agtttgacca tccgcagatg tttgttcgcc accaggcatt ccgcgaagat ccggatctcg
     9481 aaccgctggg cacgccgagt ggcctgattg agatctactc gaaaaccatc gccgatatga
     9541 actacgacga ttgtcagggg catccgatgt ggtttgagaa aatcgaacgc tcccacggtg
     9601 ggcccggctc gcagaagtat ccgttgcatc tgcaatctgt gcatccggat ttccgacttc
     9661 actcgcagct atgtgagtcg gaaacgctgc gtcagcaata tacggtagcg ggtaaagagc
     9721 cagtgttcat taacccgcag gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac
     9781 gcgtctttaa cgctcgcggt caggtgttgg caggagcagt agtttctgac cgctatgcac
     9841 ccggcgtggc gcgaattcac gaaggggcat ggtacgatcc agataaaggc ggcgagcctg
     9901 gtgcgctgtg caaatacggt aaccccaacg tgctgaccat cgacatcggg acttcgcagc
     9961 tggcgcaggc gaccagtgcg cacactacgc tggtggaaat tgagaagtac aatggaacag
    10021 tggagcaggt gacggcgttt aacg
//
LOCUS       DAJTPG010000125         9925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTPG010000125 DAJTPG010000000
VERSION     DAJTPG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..336
                     /locus_tag="OO230_003443"
     CDS             <1..336
                     /locus_tag="OO230_003443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693070.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            506..1639
                     /locus_tag="OO230_003444"
     CDS             506..1639
                     /locus_tag="OO230_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693071.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKS
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2318..2536
                     /locus_tag="OO230_003445"
     CDS             2318..2536
                     /locus_tag="OO230_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5693072.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2754..2996
                     /locus_tag="OO230_003446"
     CDS             2754..2996
                     /locus_tag="OO230_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693073.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO230_003447"
     CDS             complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="OO230_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5693074.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO230_003448"
     CDS             complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="OO230_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5693075.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO230_003449"
     CDS             complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="OO230_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5693076.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO230_003450"
     CDS             complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="OO230_003450"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8262..8264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5693077.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9043..9876
                     /gene="fdhD"
                     /locus_tag="OO230_003451"
     CDS             9043..9876
                     /gene="fdhD"
                     /locus_tag="OO230_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5693078.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2489 a         2395 c         2542 g         2499 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaatc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac
      901 cgcaatgatg agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc
      961 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
     1021 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
     1081 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
     1141 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
     1201 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
     1261 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
     1321 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
     1381 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
     1441 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat
     1501 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
     1561 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
     1621 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
     1681 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
     1741 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
     1801 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
     1861 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
     1921 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1981 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     2041 cctggtgctg ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat
     2101 atccctgaat ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat
     2161 ttttgagtaa taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa
     2221 aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc
     2281 acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct
     2341 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
     2401 acgatctgca attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt
     2461 gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa
     2521 ggagcataag gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt
     2581 ctggtattaa tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta
     2641 tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc
     2701 ggcaagggta cttttctctc catagattca aaaatgatat tggcgagata tttatgaact
     2761 ctctggcagg tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc
     2821 aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg
     2881 tagatcaata cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc
     2941 aagggtgtga ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg
     3001 aaaaaatgat gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt
     3061 gaacgcctta tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg
     3121 cctgataagc gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc
     3181 ttatcgatgt gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg
     3241 ggtaacgcgg aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc
     3301 tgcgccaccc ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa
     3361 tggaactacg ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt
     3421 catctgccac ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc
     3481 cgcagtcatc gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat
     3541 gcagtttgcc gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt
     3601 cacacaggtt gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg
     3661 acaccggcat agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg
     3721 ctttgccggg gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa
     3781 acggcgcttt atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat
     3841 cttccagctc ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc
     3901 tcatttcagg tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac
     3961 gcggcagaac gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg
     4021 cggtcagatc catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa
     4081 gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct
     4141 cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg
     4201 ccgtacgttt ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc
     4261 tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca
     4321 ggcgctggta acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc
     4381 gtagatatgc accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa
     4441 cgcgaatcgg atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac
     4501 gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc
     4561 gaagttataa cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt
     4621 cgcccaaaag atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat
     4681 gatgaacgag gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg
     4741 tgtgcccatg atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac
     4801 cgccgccagg atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta
     4861 gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta
     4921 ttctcttcct catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca
     4981 aaggtagccg caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg
     5041 gtttcgctga tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg
     5101 tgcagcacgt acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg
     5161 tcgtaaccgc gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct
     5221 ttcgtgccga agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca
     5281 accaccacgc ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg
     5341 cgcggaatgt cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac
     5401 tggaagtcga cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc
     5461 aggcagcctg gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg
     5521 tcgttctgct ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg
     5581 gggttgtcgt acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa
     5641 cacgccacct gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact
     5701 tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga
     5761 atgatatctt gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca
     5821 aggaaggact taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta
     5881 ttggcaataa agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg
     5941 gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg
     6001 gctttgatat agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt
     6061 ttattcgcca gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac
     6121 agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac
     6181 gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg
     6241 ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag
     6301 tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc
     6361 tgctgcatga taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata
     6421 tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg
     6481 ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc
     6541 catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc
     6601 tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg
     6661 tcatcgcgca attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg
     6721 gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc
     6781 gacttcggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc
     6841 gcgccaccct gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca
     6901 ccgtcagtca gcgcaatccc cggggcgtcc gcacctttcc agtgccactg caaccagcga
     6961 cctgagttaa cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact
     7021 tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta
     7081 gaggtttcag tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg
     7141 atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca
     7201 ttgaccttgc cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac
     7261 ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc
     7321 ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc
     7381 agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc
     7441 agcgtcatgt aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata
     7501 ttggagtgac cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc
     7561 agctggatca tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg
     7621 agggcataca ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag
     7681 actttcagga acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag
     7741 cgggaaacgt gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg
     7801 cgtttggcga agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt
     7861 ttttccgcgt cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc
     7921 aggctggcgt tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg
     7981 tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag
     8041 tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg
     8101 tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg
     8161 cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg
     8221 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg
     8281 tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta
     8341 ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg
     8401 gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca
     8461 aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga
     8521 aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt
     8581 gcaccgcggt tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg
     8641 ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg
     8701 caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct
     8761 acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc
     8821 ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc
     8881 ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg
     8941 gccgcaaagg tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat
     9001 accataatgt tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga
     9061 aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt
     9121 gatgatttac aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc
     9181 tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg
     9241 ctcggttttt cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat
     9301 gtcgttcctt cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg
     9361 gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg
     9421 gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat
     9481 ctcaacaaac tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg
     9541 actggttgta ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat
     9601 gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg
     9661 gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa
     9721 aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct
     9781 gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca
     9841 acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata
     9901 ttaaattgca ggacttattc atttc
//
LOCUS       DAJTPG010000126         9857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTPG010000126 DAJTPG010000000
VERSION     DAJTPG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1013)
                     /gene="bglB"
                     /locus_tag="OO230_003452"
     CDS             complement(<1..1013)
                     /gene="bglB"
                     /locus_tag="OO230_003452"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5693079.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQI"
     gene            complement(1032..2909)
                     /gene="bglF"
                     /locus_tag="OO230_003453"
     CDS             complement(1032..2909)
                     /gene="bglF"
                     /locus_tag="OO230_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5693080.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3043..3864)
                     /gene="bglG"
                     /locus_tag="OO230_003454"
     CDS             complement(3043..3864)
                     /gene="bglG"
                     /locus_tag="OO230_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5693081.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4151..4876)
                     /gene="phoU"
                     /locus_tag="OO230_003455"
     CDS             complement(4151..4876)
                     /gene="phoU"
                     /locus_tag="OO230_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5693082.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4891..5664)
                     /gene="pstB"
                     /locus_tag="OO230_003456"
     CDS             complement(4891..5664)
                     /gene="pstB"
                     /locus_tag="OO230_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5693083.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5756..6646)
                     /gene="pstA"
                     /locus_tag="OO230_003457"
     CDS             complement(5756..6646)
                     /gene="pstA"
                     /locus_tag="OO230_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5693084.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6646..7605)
                     /gene="pstC"
                     /locus_tag="OO230_003458"
     CDS             complement(6646..7605)
                     /gene="pstC"
                     /locus_tag="OO230_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5693085.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7692..8732)
                     /gene="pstS"
                     /locus_tag="OO230_003459"
     CDS             complement(7692..8732)
                     /gene="pstS"
                     /locus_tag="OO230_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5693086.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8980..>9857)
                     /locus_tag="OO230_003460"
     CDS             complement(8980..>9857)
                     /locus_tag="OO230_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5693087.1"
                     /translation="SYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHSNINYYDLN
                     PNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKAIVYFYIKK
                     TFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINNLKPVCFD
                     FKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVV
                     TSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAP
                     GQFTATATITVEIVR"
BASE COUNT         2542 a         2568 c         2280 g         2467 t
ORIGIN      
        1 tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat attcagtatg
       61 ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt catgtaataa
      121 ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc agtcatctca
      181 atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg gccacgcgcc
      241 tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa catatcctgt
      301 ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat tttcgcttcg
      361 gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg atgatggatc
      421 gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt gaatggcgcg
      481 tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg ttggtagcgg
      541 gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt agcccaaccg
      601 ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac cagcggcttg
      661 atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc cgcttcattc
      721 ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat acgcagacag
      781 gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata aaaatcgatg
      841 gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc cattacgcca
      901 tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc accttccacc
      961 tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt cataattaac
     1021 tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat ctgcgccgtg
     1081 ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg cgtcgtcaga
     1141 tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc gcctgtattg
     1201 accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt tacggtgtcg
     1261 ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat ggcgtgtaat
     1321 gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc aaccgagggc
     1381 agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc gacgtgaatg
     1441 agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg tggtgtaata
     1501 acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact ggtgatgaaa
     1561 tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc aataacgctg
     1621 gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt gaaaatactt
     1681 ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc ccccaatgct
     1741 ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc atataccgct
     1801 ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac ttttttctgc
     1861 gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat aatggcgggc
     1921 attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat acacagcggc
     1981 accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc cattaccgcg
     2041 cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat catttcgctt
     2101 atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac catcagacat
     2161 agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag tcggcgttcc
     2221 agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga gtaattcaac
     2281 aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg cccgttctcg
     2341 aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc agtaaatgga
     2401 ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa cccaaacaag
     2461 gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt ccactgaaaa
     2521 gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag accgatcaga
     2581 ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc tttctcatca
     2641 ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac cgccaggaag
     2701 acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc caccaccata
     2761 ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc ctttaactta
     2821 aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc cccgactcct
     2881 gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg cggcaaaact
     2941 ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt tcaggttttg
     3001 cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt ctttgcgcac
     3061 gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac gttgatactg
     3121 caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt aattttgttt
     3181 tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa gaatacgcca
     3241 ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt cctggtaatt
     3301 aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg tgacacctgc
     3361 aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa taaagcccac
     3421 ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg cttcttcccc
     3481 tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt tttgctgaaa
     3541 gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt cctgtaattt
     3601 tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca cctcaagagg
     3661 aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca aggcatactc
     3721 tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc caattccgcg
     3781 ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat tattgttgag
     3841 aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa aaaaaccgac
     3901 ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac aatccagtca
     3961 tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat tgcgtgcaga
     4021 gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt aactttgtgt
     4081 aattttagga atttataaag ttatatataa caaatcccaa taattaagtt attgggattt
     4141 gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc tcatcaccgc
     4201 ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa atattctggc
     4261 agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt acgctcggaa
     4321 tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct tcgtattcct
     4381 gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc atccgcgcga
     4441 acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac tccagactta
     4501 ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag attttgtccg
     4561 ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata accagtcgca
     4621 ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca tcgatcgcca
     4681 cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga tcgctgtcct
     4741 ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg cccatggtca
     4801 tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata tgtttattaa
     4861 gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga tgtagtcttc
     4921 tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact caatcaattc
     4981 gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca tgttgtgggt
     5041 gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa tacgcccggt
     5101 agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg ggcgaatggc
     5161 gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt aaccgctctg
     5221 gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc actgcacgcg
     5281 ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg cgatgttgtc
     5341 gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt tcgcacgcag
     5401 cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa tttcaccttc
     5461 cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca gcgtcgattt
     5521 accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat ccaggttgat
     5581 gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct gaattttact
     5641 cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga tgcttttcgc
     5701 taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa aagtgttaac
     5761 cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg acgcacaggg
     5821 taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg ctcatcgcaa
     5881 acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg ctccagaact
     5941 ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg gcaatcgcca
     6001 gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg gagatcatct
     6061 tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc accagtttca
     6121 gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc gcaattacgc
     6181 ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca aacagaccaa
     6241 ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact tctgccagcc
     6301 aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc ggcgtaccga
     6361 ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc agaccaccac
     6421 cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata ccgtcgatac
     6481 cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag gccatcgtcg
     6541 ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc attttgcggc
     6601 gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc gtgccccctc
     6661 attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga aggtaatcac
     6721 aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt ccgcttccgc
     6781 aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg aggcgctgtc
     6841 gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga gcgcgcggcc
     6901 cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa cgatacgcca
     6961 gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca tcaccggggt
     7021 ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga tggcgaggat
     7081 cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg cgccaacaat
     7141 cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga acagcggcgc
     7201 aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca gcagctcaat
     7261 ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga acagggcgat
     7321 accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca acgtaccgta
     7381 gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg tccataggaa
     7441 agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga caataatgcc
     7501 acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga aaattatgtc
     7561 gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt actcttcagt
     7621 taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac cgtacccggc
     7681 ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt cttccacgca
     7741 gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc gttcgcctgt
     7801 ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc ttgttctggt
     7861 ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca tgcatcttcg
     7921 cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc ttttgctgca
     7981 ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat cagtttggtg
     8041 tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc acccggcaga
     8101 cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat cggccatttt
     8161 acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc caggtagctg
     8221 gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttttgtgaa
     8281 ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt cttgattttg
     8341 cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga cttcagccct
     8401 ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa cagaccttcc
     8461 tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac ggtattagcg
     8521 ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt
     8581 tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt tgcacctgca
     8641 cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt cgcggcgaca
     8701 acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat tcataaagca
     8761 ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata tataaggaat
     8821 atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa atgtgaagat
     8881 gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa gaaatgggtg
     8941 agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa tctcaacagt
     9001 tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa ggctcgcagg
     9061 agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt tatccatgtc
     9121 tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt aaatttttat
     9181 ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt cggtagcata
     9241 aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact caatagtgac
     9301 tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa aatctgcagc
     9361 tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc aactttgcgg
     9421 agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat ccaccggggt
     9481 gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa tcaattttcc
     9541 tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg atatacttaa
     9601 aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag catgatattc
     9661 aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa catcaaggtt
     9721 cggattaaga tcataataat taatattaga gtgaacccca gcataagtta atgcctggtt
     9781 tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat cacaatgaca
     9841 gcgtccaaga tatgagg
//
LOCUS       DAJTPG010000127         9839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTPG010000127 DAJTPG010000000
VERSION     DAJTPG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            383..1501
                     /locus_tag="OO230_003461"
     CDS             383..1501
                     /locus_tag="OO230_003461"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5693088.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="OO230_003462"
     CDS             1567..1815
                     /locus_tag="OO230_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5693089.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="OO230_003463"
     CDS             1880..2248
                     /locus_tag="OO230_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5693090.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="OO230_003464"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="OO230_003464"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5693091.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="OO230_003465"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="OO230_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5693092.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="OO230_003466"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="OO230_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5693093.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="OO230_003467"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="OO230_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5693094.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="OO230_003468"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="OO230_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2410 a         2723 c         2382 g         2324 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttatggc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccagtatc acttatttaa gtgatagtct taatactagt ttttagact
//
LOCUS       DAJTPG010000128         9769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTPG010000128 DAJTPG010000000
VERSION     DAJTPG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            387..2018
                     /gene="oppA"
                     /locus_tag="OO230_003469"
     CDS             387..2018
                     /gene="oppA"
                     /locus_tag="OO230_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5693095.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2104..3024
                     /gene="oppB"
                     /locus_tag="OO230_003470"
     CDS             2104..3024
                     /gene="oppB"
                     /locus_tag="OO230_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5693096.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3039..3947
                     /gene="oppC"
                     /locus_tag="OO230_003471"
     CDS             3039..3947
                     /gene="oppC"
                     /locus_tag="OO230_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5693097.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3959..4972
                     /gene="oppD"
                     /locus_tag="OO230_003472"
     CDS             3959..4972
                     /gene="oppD"
                     /locus_tag="OO230_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5693098.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4969..5973
                     /gene="oppF"
                     /locus_tag="OO230_003473"
     CDS             4969..5973
                     /gene="oppF"
                     /locus_tag="OO230_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5693099.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6026..6355)
                     /locus_tag="OO230_003474"
     CDS             complement(6026..6355)
                     /locus_tag="OO230_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5693100.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6390..7850)
                     /gene="cls"
                     /locus_tag="OO230_003475"
     CDS             complement(6390..7850)
                     /gene="cls"
                     /locus_tag="OO230_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5693101.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7993..8166
                     /locus_tag="OO230_003476"
     CDS             7993..8166
                     /locus_tag="OO230_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5693102.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8221..9474)
                     /gene="kch"
                     /locus_tag="OO230_003477"
     CDS             complement(8221..9474)
                     /gene="kch"
                     /locus_tag="OO230_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5693103.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2560 a         2285 c         2432 g         2492 t
ORIGIN      
        1 gatgtttgtt aaggcatgga tgcaagctat agattctgat acggtcaata aaagagaatc
       61 gcttaacaat tttgcaaaat gtattggcga gtaagaaccg catttggtac tttccgggca
      121 accgccagac gattctttat tggtaatgag aataattaac aattaaagag cgtcgcgaaa
      181 gaataatgtg tctcgacagg ggagacacag tacgaatcga cataaggtga tcgtctgaat
      241 caccagaata aataaagtcg gtgatagtaa tacgtaacga taaagtaacc tgacagcaga
      301 aagtctccga gcttgtgcag ggtcccaatc cgggattaca catgctggtt aataccagta
      361 attataatga gggagtccaa aaaacaatga ccaacatcac caagagaagt ttagtagcag
      421 ctggcgttct ggctgcgcta atggcaggga atgtcgcgct ggcagctgat gtacccgcag
      481 gcgtcacact ggcggaaaaa caaacactgg tacgtaacaa tggttcagaa gttcagtcat
      541 tagatccgca caaaattgaa ggtgttccgg agtctaatat cagccgagac ctgtttgaag
      601 gcttactggt cagcgatctt gacggtcatc cagcacctgg cgtcgctgaa tcctgggata
      661 ataaagacgc gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg tctgatggca
      721 cgccagtcac agcacaagac tttgtgtata gctggcaacg ttctgttgat ccgaacactg
      781 cttctccgta tgccagttat ctgcaatatg ggcatatcgc cggtattgat gaaattcttg
      841 aagggaaaaa accgattacc gatctcggcg tgaaagctat tgatgatcac acattagaag
      901 tcaccttaag tgaacccgtt ccgtacttct ataaattact tgttcaccca tcaacttcac
      961 cggtgccaaa agccgctatc gagaaattcg gcgaaaaatg gacccagcct ggtaatatcg
     1021 tcaccaacgg tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc gttcttgaac
     1081 gcagcccgac ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc tatttgccta
     1141 ttgcttctga agttaccgat gtcaaccgct accgtagtgg tgaaatcgac atgacgtata
     1201 acaacatgcc gatcgaattg ttccagaagc tgaaaaaaga gatcccggac gaagttcacg
     1261 ttgatccata cctgtgcact tactattacg aaattaacaa ccagaaaccg ccattcaacg
     1321 atgtgcgtgt gcgtaccgca ctgaaactag gtatggaccg cgatatcatt gttaataaag
     1381 tgaaagcgca gggcgacatg cccgcctatg gttatactcc accgtatact gatggcgcaa
     1441 aattgactca gccagaatgg tttggctgga gccaggaaaa acgtaacgaa gaagcgaaaa
     1501 aactgctggc tgaagcgggt tataccgcag acaaaccgtt gaccatcaac ctgcggtata
     1561 acacctccga tctgcataaa aagctggcga ttgctgcctc ttcattgtgg aagaaaaaca
     1621 ttggtgtaaa cgtcaaactg gttaaccagg agtggaaaac gttcctcgac acccgtcacc
     1681 agggcacttt tgatgtggcc cgtgcaggct ggtgtgcaga ctacaacgaa ccaacttcct
     1741 tcctgaacac catgctgtcg aacagctcga tgaataccgc gcattataag agcccggcct
     1801 ttgacagcat tatggcggaa acgctgaaag tgactgacga ggcgcagcgc acagctctgt
     1861 acactaaagc agaacaacag ctggataagg attcggccat tgttcctgtt tattactacg
     1921 tgaatgcgcg tctggtgaaa ccgtgggttg gtggctatac cggcaaagat ccgctggata
     1981 atacctatac ccggaatatg tacattgtga agcactaatg gcaatacgtg gggcaggagt
     2041 gtcctgctcc acggtgtctg atttttatcg cattacagaa ggcacaggcc agaaggtagg
     2101 gcaatgttaa aatttattct acgtcgttgt ctggaagcga ttccgacgct atttattctt
     2161 attactattt cgttctttat gatgcgcctc gcaccgggaa gcccttttac cggcgaacgt
     2221 actttaccgc cagaagtgat ggccaatatc gaagcgaaat atcatcttaa tgatccgatc
     2281 atgacacagt atttcagcta cctgaaacaa ctggcgcatg gcgatttcgg tccatcgttt
     2341 aaatataaag attattcggt caatgacctg gtggcatcca gttttcccgt ttctgccaaa
     2401 ctgggagccg ccgcattttt ccttgcggta atactgggtg ttagtgctgg cgttattgcc
     2461 gcattaaaac aaaacaccaa atgggactat accgtgatgg ggctggcaat gaccggggtt
     2521 gttatcccca gttttgtggt tgcgccatta ttagtcatga tatttgcgat cattttgcat
     2581 tggctgccgg gcggtggctg gaatggtggg gcgcttaaat tcatgatatt gccgatggtg
     2641 gcgttgtcac tcgcctatat cgccagtatt gcgcgtatta cccgtggctc tatgattgaa
     2701 gtattacact ccaactttat tcgtactgcc cgggcgaaag ggttacctat gcggcggatc
     2761 attttacgcc atgcattaaa acctgctctg ttacccgtgc tctcctatat gggccccgca
     2821 tttgtcggca ttattaccgg ttctatggtc atcgaaacca tttatggttt gccggggatt
     2881 gggcaactgt tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt aagcctgacc
     2941 atcctggttg gtgcgttaac cattttgttt aatgccattg tcgatgtgct atatgcggtt
     3001 atcgacccga aaatccgtta ctgatactgg agctcgcgat gatgttaagt aagaaaaaca
     3061 gcgagacgct ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc ttgtggcagg
     3121 acgcacgtcg acgttttatg cataaccgtg cggcggttgc cagtctgata gtgctggtgc
     3181 tgatcgcgtt atttgtaatc ctggcaccga tgctttccca gtttgcctat gacgatactg
     3241 actgggcgat gatgtccagc gccccggata tggagtccgg tcactacttt ggtactgact
     3301 catccggtcg cgacctgctt gtgcgcgttg cgattggcgg gcgtatctca ctcatggtcg
     3361 gtgttgctgc ggcactggtg gcagtggtcg tggggacact ttatggttcg ctttccggtt
     3421 atctgggcgg taaagtggat tcggtaatga tgcgtctgct ggaaatcctc aactccttcc
     3481 cattcatgtt cttcgtcatt ttgctggtga cctttttcgg ccaaaacatc ctgctgattt
     3541 tcgtggcgat tggcatggtt tcctggctgg atatggctcg tattgtgcgt gggcaaaccc
     3601 tgagtctgaa gcgcaaagag tttattgagg cggcacaagt tggcggtgta tcgacgccgg
     3661 gcattgttat tcgccacatt gtgccgaacg tactcggtgt ggtggtggtc tacgcatcgc
     3721 tactggtgcc cagcatgatc ctctttgaat ctttccttag cttcctgggg ttgggtacgc
     3781 aagagccgtt aagcagctgg ggggcattgc tgagtgatgg cgcgaactcg atggaagtct
     3841 ctccatggtt actgttgttc ccagcgggat tccttgtggt gacactgttt tgtttcaact
     3901 ttatcggcga tggcttgcgt gatgccctcg acccgaaaga tcgttaagga gtgcagccat
     3961 gagcgtaatt gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg cactgctgaa
     4021 cgtgaaagat ttgcgtgtca cctttagtac cccggacggc gacgtcacgg cggtcaatga
     4081 tttaaatttt tccctacgtg ccggagaaac gctgggcatt gtaggtgagt ctggttcggg
     4141 taaatcgcaa actgcatttg cgttgatggg cctgttggct gccaacggac gcattggcgg
     4201 atcggcaacc ttcaatgggc gtgaaatcct caatttgcca gagcgtgaac tcaataaact
     4261 gcgcgctgaa caaatctcaa tgatttttca ggacccaatg acttcgttga atccctatat
     4321 gcgcgtcggt gagcagttga tggaagtgct gatgctgcat aagaacatga gcaaagctga
     4381 ggcgtttgaa gagtcggtgc ggatgctcga tgcggtaaaa atgccggaag cacgtaaacg
     4441 catgaaaatg tacccgcacg aattttctgg cggcatgcgt cagcgagtca tgattgcgat
     4501 ggcattgcta tgtcgaccta agttgctgat tgcggatgaa cccactacag cgctggacgt
     4561 caccgtacag gcgcagatca tgacgctatt gaatgaactg aagcgggaat ttaatactgc
     4621 catcattatg atcacccacg atcttggcgt ggtggcgggg atctgtgaca aagtgctggt
     4681 aatgtacgcc gggcgcacga tggaatatgg caatgcgcgc gatgtctttt atcaacccgt
     4741 tcatccttat tctatcggtt tgctcaacgc ggtgccgcgt cttgatgcgg aaggtgaaac
     4801 aatgttgacc atcccgggta atccgccaaa cttgctgcga ttaccgaaag gttgcccgtt
     4861 ccagccacgt tgtccacatg cgatggaaat ttgtagtagc gcaccgccgc tggaagagtt
     4921 tacgcctggc cgtctgcgtg cttgctttaa accggtggag gaactgttat gaatgctgta
     4981 actgaaggaa gaaaagtcct ccttgaaatc gccgatctta aagtgcactt tgaaatcaaa
     5041 gatggcaaac agtggttctg gcaaccgccg aaaacgctca aagccgtcga tggtgtcact
     5101 cttcgcctgt atgaagggga aacattaggt gtggtagggg aatcgggatg cggtaagtcc
     5161 acctttgctc gcgccatcat cggtttggtc aaggcgaccg acggtcatgt tgcctggtta
     5221 ggtaaagagt tgctgggcat gaagcccgat gaatggcgtg ccgttcgcag tgatattcag
     5281 atgattttcc aggatccgtt ggcatcgcta aacccgcgta tgaccatcgg cgagatcatc
     5341 gctgaaccac tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg cgagcgcgtg
     5401 aaggcgatga tgctgaaagt cgggttattg cctaacctga ttaaccgcta tccgcatgag
     5461 ttctccggtg ggcagtgcca gcgtatcggg attgctcgtg ctcttattct tgaaccgaag
     5521 ctgattatct gcgatgagcc ggtttcggcg ctggacgtgt caattcaggc gcaggtggtt
     5581 aacctgctcc agcagctgca acgtgagatg ggattgtcat taatttttat tgctcatgac
     5641 ctggccgtgg taaaacacat ttccgatcgt gtgttggtga tgtatctcgg ccatgcagta
     5701 gaactgggga cctatgatga ggtctaccac aatccactac atccttacac cagggcattg
     5761 atgtcggcag tccccatacc tgatccggat ctggagaaga acaaaaccat ccagttactg
     5821 gaaggggaat taccgtcgcc gatcaacccg ccttccggtt gtgttttccg tacccgttgc
     5881 ccgattgccg gtccggagtg cgccaaaaca cgtcctgttc tggaggggag tttcagacac
     5941 gccgtttctt gcctgaaagt cgatccgctt taaaaacaat aagggctgac agttgtcagc
     6001 cctttttcac gctaaaagcg attatttatt cccgccagat gatatggcaa agtttgtgat
     6061 ctttttcgcg gcataacaga atgcgggcga aaacatcgct gatttcacca tcttcactgt
     6121 ccgccagacc aatcaccact tcggcaaaaa agtccgggtt caaatcgaaa tcaacatgtt
     6181 cctgccagtc ttccgccgga tcgaataact ccgcgccgcc gcgctcttca aactgaagat
     6241 tgaacagcag aacatcagct ggatcgagat tgtcggcagc cagttcgaga aaaatatcgt
     6301 aggcctgctc aagcgtttca tcttcagtca ggcgattgtt tagatccata tccatagtca
     6361 ctacctgttt aacctctgtt ggcgacgttt tacagcaacg ggctgaagaa gtaaaacagt
     6421 cgctcggcga cacgttgcca taatggacgt tttagccata aacgggcatc gagcagacgt
     6481 gaacgcgaaa tataatcgtc ctgaacggcg gcaaggtctg caccaaaacc tttatcgtcg
     6541 attgccaagg taatctcgaa attcagccac agactacgca tatcaaggtt aacggtgcca
     6601 accagactta gttcgccatc gaccagcacg ctcttggtat gcagtaaccc gccttcaaac
     6661 tgataaattt taaccccagc agcaagcagt tccgtaaaga atgcgcgact ggcccagccg
     6721 accagcatcg agtcattttt tcgcggaagg ataatactga catccacccc gcgctgcgcc
     6781 gccgtgcaaa tcgcatgaag taaatcatcg cttggcacaa agtagggcgt ggtcatgatc
     6841 aaatattcat gcgccgaata agccgcagtc aataatgcct ggtgaatgag atcttccgga
     6901 aagccggggc cagaagcaat tgtgtgaatg gtgtgaccgc tggcctgttc aaacggcata
     6961 atattgacat ctggtggtgg cggcagaata cgttttccgg tttcaatctc ccagtcgcag
     7021 gaataaataa tccccatcgc ggtggcgatg gggccttcca tacgcgccat cagatcaatc
     7081 cattgcccta cgcccgcatc ttgtttgaag tagcgaggat cgaccatatt catgctgccg
     7141 gtgtacgcga tgtaattatc gatcatgatc atcttgcgat gttggcgcag gtccatacgg
     7201 cgtaaaaaca cacgcatcag attgaccttt aaggcttcga ccacttcaat accggcatta
     7261 cgcattagct cgggccacgg gctgcggaaa aaagccacac tcccggcgga gtcgagcatc
     7321 aatcggcaat gaataccacg ccgtgcagcc gccattaatg attcagccac ctggtccgcc
     7381 atgccgccgg gctgccagat ataaaacacc atctcaatat tatggcgcgc gagctggatg
     7441 tcgcggatta acgcctgcat cacatcatct gactcggtca tcagctgtag ctgattccct
     7501 ttgaccccag cgatcccctg acgacgctcg caaagcttga ataatggcgc agcgacactg
     7561 ctattttctt cggcgaagat atgcttacag gctttaaggt cgttaagcca ttttgcggtg
     7621 gaaggccaca tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag ctcgccaacg
     7681 gcaagatagg caataattcc gactaacggc agaatgtaaa taatcaacag ccaggccatc
     7741 gcggagggaa ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc aatgagcaac
     7801 cagtatccca gaatggccaa ccaactcacc aacgtataaa cggttgtcat agattagaaa
     7861 tccttttgaa agcgcattgt tatgagttta cgcatatgga ttcatctggc aaataaaaac
     7921 gcgggaaaag cactggtcag cgtcgggggc gggggtataa tgaccattct gttattgcat
     7981 agagtagtta acatgaagcg gagtagaacg gaagtggggc gctggcgcat gcagcgtcag
     8041 gctagccgac gtaaatcgcg ttggcttgag gggcaatcgc gccgaaatat gcgtatccac
     8101 agcatcagga agtgcattct aaacaaacag cgtaactcgt tattgtttgc gatctacaat
     8161 atctaaatgt actgggcacc attgcggtgc ccaataagtt tactcttccg tctttaaact
     8221 ctatttttgc gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctgaa
     8281 aataccatga cctgagttca acaacattga aacgagcata tcattattaa tctcttcacc
     8341 atttaaaact cgcgccagaa tttcgctgcc aaacagttgc ggtgagagaa tgatatccgg
     8401 atgtaccatc ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac
     8461 atcactgctc atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact
     8521 cagcgccaga atggcccggc aacgatcgat tcccgctttc tttaatactg aactgtcatt
     8581 actgtcaccg gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc
     8641 atcttcaggc aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat
     8701 cgtattgatg gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt
     8761 atgattgttt ccttttaaaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga
     8821 ggtcatagat gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg
     8881 tgctgattca gaaacaggga caatatctcc atagccgacg gttgacatgg tttctatcga
     8941 aaaatagaac gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag
     9001 cgcaccgtag gttgagtaaa acagtaatgt cgtgaaacta ataaatgcaa aaattgtccc
     9061 ggctgcggcg ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa
     9121 aatgcaaaat ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc
     9181 tatcaacaac aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat
     9241 ggagttcagg acaagaaaga cgccgagcat aaatagtggg gcgtttgcca gcaaggagag
     9301 atccaactct gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata
     9361 gattgtttta aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg
     9421 tagggtcacc cataaatttg ttgctgtttg tttgaatgta gcccagtgac tcactgatac
     9481 ctctgtaacc acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca
     9541 gcgtgtgaat gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag
     9601 tgagcaactc tgcgtcagga aagggctttt ttcctgaaca tgctcacatc atataaagaa
     9661 atacagataa agttattatc tgcttgtggt agtgaatgca ctgaccggct ataaggaaag
     9721 gccaaacaag aacacggttg caaaaaccgt gcccttaaat attggatct
//
LOCUS       DAJTPG010000129         9579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTPG010000129 DAJTPG010000000
VERSION     DAJTPG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..310)
                     /gene="psiF"
                     /locus_tag="OO230_003478"
     CDS             complement(<1..310)
                     /gene="psiF"
                     /locus_tag="OO230_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5693104.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     K"
     gene            complement(429..1844)
                     /gene="phoA"
                     /locus_tag="OO230_003479"
     CDS             complement(429..1844)
                     /gene="phoA"
                     /locus_tag="OO230_003479"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5693105.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQNAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1945..2205)
                     /gene="iraP"
                     /locus_tag="OO230_003480"
     CDS             complement(1945..2205)
                     /gene="iraP"
                     /locus_tag="OO230_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5693106.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2387..2590)
                     /locus_tag="OO230_003481"
     CDS             complement(2387..2590)
                     /locus_tag="OO230_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693107.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2668..3762
                     /gene="ddlA"
                     /locus_tag="OO230_003482"
     CDS             2668..3762
                     /gene="ddlA"
                     /locus_tag="OO230_003482"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5693108.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3786..3998)
                     /locus_tag="OO230_003483"
     CDS             complement(3786..3998)
                     /locus_tag="OO230_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5693109.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4258..4566
                     /locus_tag="OO230_003484"
     CDS             4258..4566
                     /locus_tag="OO230_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5693110.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4625..5719)
                     /gene="yaiW"
                     /locus_tag="OO230_003485"
     CDS             complement(4625..5719)
                     /gene="yaiW"
                     /locus_tag="OO230_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5693111.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5732..6952)
                     /gene="sbmA"
                     /locus_tag="OO230_003486"
     CDS             complement(5732..6952)
                     /gene="sbmA"
                     /locus_tag="OO230_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5693112.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7304..8461
                     /gene="ampH"
                     /locus_tag="OO230_003487"
     CDS             7304..8461
                     /gene="ampH"
                     /locus_tag="OO230_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5693113.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8462..8965)
                     /locus_tag="OO230_003488"
     CDS             complement(8462..8965)
                     /locus_tag="OO230_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5693114.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9053..>9579)
                     /locus_tag="OO230_003489"
     CDS             complement(9053..>9579)
                     /locus_tag="OO230_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5693115.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2378 a         2445 c         2278 g         2478 t
ORIGIN      
        1 tcttgaggca ggcactcata aacttattac gatcatcacc tttcagagct tgttgcgttg
       61 cttgattatt acattcacgc atcttttgct gctgtggcgt caaacttttt tcaccaggcg
      121 cagacttgct gttcttcagg caatcactca tgtaggtctt acgagcatcc cctttcaacg
      181 cctgcgccgt cgcctgctga ttacaggagg tcatacgctg ttgttgtggg gttaaggttc
      241 tctcggcagc gccgacggtg gttaaaaaaa ccagaccaaa aagcaaggta accagtaatg
      301 ttattttcat agcaccatcc ctcttcatgt tttaaccatg agcgtttgcg cccgtgatct
      361 gccattaagt ctggttgcta acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg
      421 gcgcagtttt atttcagccc cagagcggct ttcatggtgt agaagagatc ggtctggtcg
      481 gtcagtccaa caacattggc ggcatgcggg ccatacgccg caatacgcaa ctgactgccg
      541 gtatgttctt gtgaatcctc ttcggaattc ccgtaactca tcaccatcac tgcgccatct
      601 ttagtattta gcgcctgagt gaggcccggc gctttggtat ccggcgcaac aacctggctg
      661 gcgtgggcgt gatcagcggt gactatgacc agcgtgttac cctccttttt agcgaattcc
      721 agcgcccgtt gtacggcttc atcgagatcg accgtctcgc caatttgccc acaagggttc
      781 gcagcgtgat cctgtttatc gattgacgca ccttcaactt gcaggaaaaa tcctttctca
      841 tttttactca acaattcaat ggctttgtcg gtcatctgtg ccagggttgg tacactgtca
      901 ttacgttgcg gattaggcgt ataggtgact gcgggcttat cgatattgcc gtggtacgtt
      961 gctttcggcc ctagccagcg cactggcata ttgccgtcag caaacaggcc aagcaggggt
     1021 ttttgctgat tcgcttccgt caccgaattc agtgaggcag catcgttcac caactgataa
     1081 ccacgcgcct gtgcctgttc acgcagcgtt tttccctgcc attcaccagc ggttgccgtt
     1141 tcagcaaagg tttttgcgcc gccgccaagc gtaacgtcgg cacgagcgtt aagcagctgt
     1201 tcggtaatcg atccttttcc gcctttttcc agagcgttac ccggacattt ttcactggtc
     1261 gcgctcggac cgtagcattt gcgcgaagtc acatgtgcca ccagcgcagc gggcgtggca
     1321 tcctgcaact ctgcggtaga aacgttaccg gtcgccagac ctgcggcttt tgccatttcc
     1381 agaatcgttg ggtgatcttt ttcgtgaata tcgacgccca gcgcgccgtt ataggttttg
     1441 acatcggttg accaggcggt tgctgatgca gccgagtcgg tgacgtagtc cggtttgcca
     1501 gtttttttat tcagcgcata gtgagtgtat tgcccggtaa gcggtaaggc atcgatacct
     1561 ttaaaaaagc cgcccgcacc ttcggcataa ttacgtgcgg cagtaatttc cgaatccccc
     1621 atcccatcgc caatcagcaa aataatattt tttgcaggtt tatcgctaag agaatcacgc
     1681 agagcggcgt tctgatcacc cgttaagcgg cgagcaccgc cgggtgcagt aatatcgccc
     1741 tgagcagccc ggttttccag aacaggcatt tctggtgtcc gggcttttgt cacaggggta
     1801 aacagtaacg gtaagagtgc cagtgcaata gtgctttgtt tcactttatt ttctccatgt
     1861 acaaatacat taaaaaataa aaacaaagcg actataagtc tcggccgtga caactttatg
     1921 acagctgttg aaaagattaa ctttttactg acaaggatgc ttcaataact tctttacgta
     1981 atcgcgcagc agctctgtat cgtcgtcagg aatgctggca tcgggcttta cctcgtacag
     2041 cgccccctct acctgatcaa tcaaccgctg ttggtcattt tgcgccatat tgcgaagcat
     2101 tgcagtgacg ataatctcca aagcttctac ctgcgcacac agttctttcg actcttcttc
     2161 tttttgggca agcttaaata acaactcagc aatgagattt ttcatgtctg tatttcctta
     2221 tccaaagtat ggagaagtta acattatgtt tcacaacttc atagtgtctg gaagtattat
     2281 ttttatgaca aaggaaatac tttgcgcaaa tattttttgt ttgattgcca gcgaaacgtt
     2341 tctcttattt aaataattac ccacacaaaa tataggcttt taattatcaa cctttattat
     2401 aaataaaagc tatgaaatat attctcttcg ttaacttata attaagagaa aaaaactcac
     2461 cgcattgcca tttaagccag gaataaatta gcttcccgtt catcattttt gtgatcttgc
     2521 gcacatcttt gagataacgc actttcttca ccagtacgtt gcacgctttg tcagcagaag
     2581 attgcaacaa tctttcagaa ggccgcgaat cccccgtttt gtcgtggtaa tgtatgcact
     2641 ttgtgttaga cgggataggt ttttaagatg gaaaaactgc gggtaggaat cgtttttggt
     2701 ggtaaatcag cggaacatga agtgtctctg caatcggcaa aaaacattgt cgatgccatt
     2761 gataaaagtc gcttcgacgt tgtgctgctg ggcattgata aacaagggca atggcacgtc
     2821 agcgatgcca gcaattatct gctaaatgca gacgatcctg cccatattgc gttgcgccct
     2881 tcggcgacca gccttgcgca ggtgccaggt aaacatgagc atcagcttat cgacgcgcaa
     2941 aacggtcagc cgttgccgac ggtggatgtc attttcccga ttgtccacgg tacgctgggc
     3001 gaagatggtt ccttgcaggg aatgctgcgg gtcgccaatt taccgtttgt aggttctgat
     3061 gttctggctt cagcagcctg tatggataaa gatgtcacca aacgtctgct acgcgatgcc
     3121 gggctgaaca ttgcgccatt tattaccctg acgcgcgcta atcgtcacaa catcagtttt
     3181 gccgaagtgg agtctaaact ggggttacca ctgtttgtaa aaccggctaa tcagggctct
     3241 tctgttggtg tcagcaaagt aaccagtgaa gaacagtacg caattgccgt cgatctggcg
     3301 ttcgagttcg atcataaagt gatcgttgag caagggatca aaggtcgtga gatcgaatgc
     3361 gcagttctgg gcaacgacaa tccgcaagcc agcacctgtg gcgagatcgt actcaccagc
     3421 gatttctatg cctacgacac caagtacatt gacgaagatg gcgcgaaagt ggtagttccg
     3481 gcagccattg caccagagat caacgataag atccgggcga ttgccgttca ggcttatcag
     3541 acgttgggat gcgcaggcat ggcgcgtgta gacgtgtttt taaccccaga gaacgaagtg
     3601 gtgatcaacg agatcaacac actgcctggc ttcaccaaca tcagtatgta tccgaagctg
     3661 tggcaagcca gcggtctggg ttacaccgat ctgatcacac gtttgattga gctggcgctg
     3721 gagcgtcacg ctgcggataa cgcattgaaa accacaatgt aatattttaa cgcccgataa
     3781 tattcttatt gggcgttttc agtttcttca tcgcgacgac gaataatcaa ccccgccagc
     3841 cagaaactaa tcacccacgt caccagaccg acggcatagg tctgccaacc ttttgcttca
     3901 aatcccagta cccccaccac gccattaaga atgaatataa ggccaatggc gaacgcatag
     3961 tagtgccagt cacggcggat ttttacaggc agattcatgg cagctccgtt aacgaaaaac
     4021 aacatccttg catattctgt aacaaccgtc tgtggtgtag acaacatttt cgtaattatt
     4081 aataaattta acttaaggtt gccattttgt gactcacaac gaagattcac gcttcgggaa
     4141 tattcccaac atcaatttac catgattctg atattgacaa tctaaatgac ctgtcagact
     4201 tgccataagc cgctggtatc acaccagaag agaagcagat gacttccgga ggtttttatg
     4261 gctgatttca ccctgtcaaa atcgctgttt agcggaaaat atcgcaatgc ctcttcaacg
     4321 cctggcaaca ttgcctatgc gttgtttgtg ctgttttgct tttgggctgg ggcgcaattg
     4381 ctgaacctgt tagtgcatgc gcccggcgtc tatgagcgtt taatgcaggt ccaggaaaca
     4441 ggtcgcccac gggtggaaat tggtttaggt gtcggcacca ttttcgggct gatcccgttt
     4501 ttagcaggct gcctcatttt tgcagtggtg gcgctatggc tgcactggcg acatcgccgc
     4561 cagtaaaatt tacgttgcca gatgcggcct gaaacgcctt atctggcctg acattcctgt
     4621 ttgatcactg tttcatacaa cgcgcccgcc gttcgtctac gcgcttcgca aaccaggccg
     4681 tagtcaggtt gcgggtgatt ttcgggcttt ccagttgaat gccaggcaac atttcgcggg
     4741 gtagtgattt acccgttttc gcttcggcaa gttgataaac tttcttgtac agcgccgtct
     4801 cctcaaaaga gaagctgtca cctttctcta actgacggcg aatttcgctg tcgctcatcc
     4861 ccagttttcc cgccagtttg cgcgttgcca gttcggtttt cccgggttct ttactgtcgt
     4921 aacgaattaa atcaccgtcc agtgccagct tcacgccgct ggccttactg actgcgttct
     4981 gaaacgcggc attacggctg gcgtaccagc cagcgttaaa atcagcaaag cggtatatcg
     5041 gtgcgctata gctggcggga taattcagta aatggtaagt accaaaccac aacccgccgc
     5101 ggcgactgaa gacttcctga cgaactgtac cgtccatttt ccacggatac cctttggtat
     5161 gctgttcggc aaaagcaatg ctgacctgca tcggcccacc ggtgcgcacc ggattaagcg
     5221 aaccaaacag cgtctgcccc attggtacca tgctgatcag atcgtcaaaa atcgcgctca
     5281 attgcttttc tgtacgcacc gaatctaacc gttcgctata acttttgccg tttggcgatt
     5341 tgatttttag cgcggtatga accagaaaag caggaatgtg catccgttca gcacgacggt
     5401 caatctcttg ccaggcgatc ttgcttaagc ccggaacggc cggatctgcc tgataatttg
     5461 actcctgctg cgccaccgcc agcaccgagt agacgttttc cagcgtcggt gccagcccct
     5521 gactttcaaa ggtggtggcg agatctttcg cccaggcatc gcggtctttc acgctcgcag
     5581 gcatcttctg ccgcacgacg ctcgctacat ctatcgcttt ttcgcccttt ttcaacggtt
     5641 gcggagcctg actactacac cccgctaaaa ccagaaccgc caacaatgac agcgaagata
     5701 gatgattgac gcgcgacatt acttctcctt tttagctcaa ggtatgggtt acttcctgaa
     5761 ttttgtcacc atccagctca tgttcaaagc tgcgcagacg tttgtagata gacatcaact
     5821 caaccagtgt ggtccatgag ttaatcaggt actggaaagc accgcgaacc tgaccaaaaa
     5881 cgttggtaat ctgcgtcatc aggccgagcg taatcgtacc ggcaacaatt gacggaaaca
     5941 gcaagaacaa accgaaaacg ttatcgacct gcaaatagag aatgcgggcg atgttgaaat
     6001 acatatagtg aaaatagagg cgaaaatagt ttttccgtac ggcgctaaac agctcgcgta
     6061 ccgtaggcgg cgtcgcgcgc gtggcatcgt cttcaccata aaccagctct ttacggtagg
     6121 cggcctctac tcgctggttt ttaaactcca gccccggcag tttgatccct accactgcca
     6181 gcaatccggt ccccatcagc gaccagacga tagcggcaat caccagacca tacggaatgt
     6241 gcccgataat cggcagctcc ggcacatgcg cggagagcgt taccagcacc ggcaggaagg
     6301 cgatcaacgt catgatggcg ttgataaaac tgacgcccat attctccagc gttgaagcaa
     6361 aacgcatggt gtcttcctgc acacgctgtg cggcaccttc gatatgacgc agttgttgcc
     6421 agttcgccat gtaatattcg ttcatcgccg tacgccagcg gaacacgtag tgactgacaa
     6481 agaagttgtt cagtacactg atcaccaccg cgatcagcgc aatccccaga aagacgccca
     6541 cttcgcggta aaattgttcg atagtgactt tatgcggcga acttagcgcg gtttgaatca
     6601 gatcatagaa cggcgcatac caggcgttga cggcgacccc gacttccacc aaaaaccaag
     6661 tgacgaagat aatcagcgca gtaccgagta ttgaccagta ttgccaacgg tgcgggctgt
     6721 agataaacca gaaaaatgca aaaagtccta cgcaaacaat gtagtaagcg taaaaaatca
     6781 ggaaatccaa cgaccagaaa cgcgcggcgc taatcgggat ctgcccggaa gcgccggtga
     6841 tacgtgccac ccagtcaccc ccaccggctt gccagaagat aacggcaatc aatgcccaaa
     6901 caaaggccga gagaaaaaac gttcccggct ttgggaaaaa agacttaaac atatgcactc
     6961 ctgctaactt cttatcgttt taacaactgc tgtgtattaa ccgcatgacc gctacaaagc
     7021 gtcagccaaa tgaaaccgcc cattgtgacc catagcaagg cgcttagttc gccagcctga
     7081 ccataagaat tgtttcgacg gatttcacaa accaaagatt gtttacataa gcggattaac
     7141 acgcgcagga tcacaaatgc aggcattggt gagcaggaaa aagatcgtgt ttacgcgccg
     7201 ttacgttttt aacactatta ttgcactaat tcgccctttg caatccatca atgagtagta
     7261 taaatacgct cagttacctt cattcaatct atggacacca ccgttgaaac gtagtctgct
     7321 tttttctgcc gtgctgtgtg cggcgtcatt gacctccgtc catgcggcgc agccgatcac
     7381 tgaaccggag tttgcctctg atattgtcga tcgttatgcc gatcatattt tttacggcag
     7441 cggtgccacg gggatggcgc tggttgttat cgacggtaac cagcgcgtct ttcgtagtta
     7501 tggtgaaacg cgacctggta ataacgttcg cccgcagctg gattcggtcg tgcgtattgc
     7561 ttccctcacc aaactaatga ccagtgaaat gctggtgaaa ttgctcgacc aggggaccgt
     7621 aaagttaaac gatccgctaa gtaaatatgc tccgccaggc gcacgcgtgc cgacctacaa
     7681 tgggacgccc attaccctag tgaatctggc aacccatacc agcgccctgc cccgtgaaca
     7741 gcccggtggc gcggcacatc gtccggtgtt tgtctggcca acgcgcgagc aacgctggaa
     7801 atacctttct acggcgaagc tgaaagccgc gccaggcagt caggcggcgt actctaacct
     7861 tgcgttcgac ctgctggcag atgcactggc gaatgcgtcc ggcaagcctt atacccagtt
     7921 gtttgaagag cagattaccc atccattggg gatgaaagac accacctaca ccccctcacc
     7981 ggatcagtgc cgccgtttaa tggttgctga gcgcggtgcc agtccgtgca ataacacgct
     8041 ggcggcaatt ggcagcggcg gcgtctattc cacgcctggc gatatgatgc gctggatgca
     8101 gcagtatttg tcatctgatt tttatcagcg cagtaaccag gcagatcgca tgcagacgtt
     8161 gatttatcag cgcgcgcagt ttacgaaagt gattggcatg gatgtccccg gcaaagcgga
     8221 tgcgcttggt ttaggctggg tatatatggc cccgaaagag ggtcgtccga ggattattca
     8281 gaagacaggc ggtggtggcg gtttcattac ctatatggcg atgatcccgc agaagaatat
     8341 tggcgcgttt gtggtggtca ctcgttcgcc gttgacgcgc tttaaaaata tgagcgatgg
     8401 catcaatgat ctggtgaccg agctaagcgg gaataaaccg ttggttatcc ccgcatcctg
     8461 attaatactc tgaaggcaaa cggttgatag cgaccagttt gcctttattc atttcgatat
     8521 agccgccttt acgcaaagca gcgagaactt ctgcgacgac agaacgcgat atgcgtgtac
     8581 gttgatggat ataattcatc acaccaatac gtgatcgcaa ttcttcattc cagtcaatca
     8641 ttgataataa tgttgcgcgg gtttgtttgg ctggtagatg atatcccgta caatttcctt
     8701 ctgatattaa tttgtatggt atatcgtttt tcattaaacc atcagccagc cccataatat
     8761 aaggagcctg ggtaataccg ataagtacgt tttcttctct acgcagagag ataactcctt
     8821 ctaaaataac gaaagtatct tcgttattga catcactgga aaatataact tgcttttcat
     8881 tattaaactc gaagcgcgta ccgtatctgg acaaacattt atcgagctta ccaaattcct
     8941 gaagaggttt aactacagat aacatttgcg cgtcctttgc agtaatgccc gtcaaatcct
     9001 tgacgggcat tatttagatt aaattaccag tatttcttcg gagtgaagaa tattaccaag
     9061 tatatttaac acccacgttc gcggaccagt cttgatctac gtcaccacca ccgaggtagt
     9121 tagcatcggt ataggcgctg aagttcttgg tgaagctgaa ctgagtaccc agaccaacac
     9181 gtaccgcaga cccttcagta ccgttatcga tggaatcacc gttcacatcg ttatcgttgt
     9241 tagagtcgtc gtagacgtaa gccagtttga agtacggagt cagagcctga tcttcgctgt
     9301 aggtgaaggt ataacctgca tctacaccca gttcataacg catgctgtcg taaggctgac
     9361 cgtcaacttt catgtcgttg ctcagctggt agtcatcacc agactggaac agaccagaaa
     9421 tgctgccgta aggagtcacg taaccagcat cacccagttt gaagtcgtaa ccggttttca
     9481 aaccgaagcc ccaagcgtcg gagttggtgc taccgtcaac gtaagtaccg ttgctcatgg
     9541 ttgcagacag gtcgttgttg aagtgagagt agctcaagc
//
LOCUS       DAJTPG010000130         9536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTPG010000130 DAJTPG010000000
VERSION     DAJTPG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(286..1263)
                     /gene="epmA"
                     /locus_tag="OO230_003490"
     CDS             complement(286..1263)
                     /gene="epmA"
                     /locus_tag="OO230_003490"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5693116.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1588..3396
                     /gene="frdA"
                     /locus_tag="OO230_003491"
     CDS             1588..3396
                     /gene="frdA"
                     /locus_tag="OO230_003491"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5693117.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3389..4123
                     /gene="frdB"
                     /locus_tag="OO230_003492"
     CDS             3389..4123
                     /gene="frdB"
                     /locus_tag="OO230_003492"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5693118.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4134..4529
                     /gene="frdC"
                     /locus_tag="OO230_003493"
     CDS             4134..4529
                     /gene="frdC"
                     /locus_tag="OO230_003493"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5693119.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4540..4899
                     /gene="frdD"
                     /locus_tag="OO230_003494"
     CDS             4540..4899
                     /gene="frdD"
                     /locus_tag="OO230_003494"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5693120.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4962..6095
                     /locus_tag="OO230_003495"
     CDS             4962..6095
                     /locus_tag="OO230_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5693121.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6184..6717
                     /gene="blc"
                     /locus_tag="OO230_003496"
     CDS             6184..6717
                     /gene="blc"
                     /locus_tag="OO230_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5693122.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6714..7031)
                     /gene="sugE"
                     /locus_tag="OO230_003497"
     CDS             complement(6714..7031)
                     /gene="sugE"
                     /locus_tag="OO230_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5693123.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7207..7353)
                     /gene="ecnB"
                     /locus_tag="OO230_003498"
     CDS             complement(7207..7353)
                     /gene="ecnB"
                     /locus_tag="OO230_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5693124.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7464..7589)
                     /gene="ecnA"
                     /locus_tag="OO230_003499"
     CDS             complement(7464..7589)
                     /gene="ecnA"
                     /locus_tag="OO230_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5693125.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7641..8207)
                     /gene="efp"
                     /locus_tag="OO230_003500"
     CDS             complement(7641..8207)
                     /gene="efp"
                     /locus_tag="OO230_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5693126.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8249..9277
                     /gene="epmB"
                     /locus_tag="OO230_003501"
     CDS             8249..9277
                     /gene="epmB"
                     /locus_tag="OO230_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5693127.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2403 a         2458 c         2438 g         2237 t
ORIGIN      
        1 caaatacgga agtaaagatc atcaatatga gtcctatcag actcatcttt tttatcgtgt
       61 gaggcatgaa accatccttc atttcagcgc gtgtataaca cgtgcccact gaactgcact
      121 gcgcaacatt gagcaggcag cgtaaaaacc aatacagaat tgatacttgg atgatatcaa
      181 atatccagtc ttaaattgtg gcctttaacg gaaaaatgaa aacatatgct gcagaatggc
      241 gcttatcacg ccattcttcg ctgttaattc agtaattttt cagaattatg cccggtcaac
      301 gctaaaggcg atgacttcag ccagtgtctc cgcgcccagc gccaacatca ccagacgatc
      361 aacacctaat gccacgccgg aacagtcagg cataccgact ttcaaggctt caatcagatt
      421 ctggtcaatg gggtgctgcg gcaaaccgcg cgccgcgcgc ttacggttat cttgttcaaa
      481 gcgttgttgc tgctcacggg catccgttaa ttcatggaaa ccattcgcca gctcaatacc
      541 tttataataa acctcaaagc gttcagcgac ccgatgatct tcggtactga tttgcgccag
      601 tgatgcctgg ctggctggaa agtggtacac aaaggtcggt ttttctttac caatatttgg
      661 ctctacgcca aaggtaaaca gcaattgtag caacgtgtcg cggtcttctt cggtatcagc
      721 aacattgctc aaatccagtt tcgctgcgac ttcccgcagt tgcgttttgt cggcagagag
      781 cgggtcaatt tccagataac gcaagaaagc ttgttgataa gaaaggcttt ctgctgccgg
      841 gcagtccagc acctgttgta agagatcgtc cacctcgttc atcaaccggt acatatcata
      901 gtgcggtcga taccactcca gcatagtgaa ctcagggttg tgataacgcc ccatctcttc
      961 attacggaag ctgcggcaca gctggaatac cggcccacaa ccagcaacca gcaggcgttt
     1021 catatggtat tccgggctgg tcattaacca gagattcatc ccctgcgaat gcccggggcc
     1081 aacgaaacgt gtctcaaacg ggaccaaatg aatatcggtt accgtcgcct ggctcataca
     1141 aggcgtctcc acctccagca ctccacgatc ggcaaagaaa cgacggatct ccgccataat
     1201 cgccgcgcgt tttaataagt taggaatgga tgcgctcggc tgccaggatg ccgtttcgct
     1261 catagttaaa tctccagttt ttgacaaggg cacgaagtct actcgcaacg cgacggcgag
     1321 acaaatttta cgcaggaatc aaacagcggt gggcagtgac taaaaaaagc acgatctgat
     1381 ggtttagtaa ttaaattaat catcttcagt gataatttag ccctcttgcg cactaaaaaa
     1441 atcgatctcg tcaaatttca gacttatcca tcagactata ctgttgtacc tataaaggag
     1501 cagtggaata gcgttcgcag accgtaactt tcaggcactt accctgaagt acggggctgt
     1561 gggataaaaa caatctggag gaatgtcgtg caaacctttc aagccgatct tgccattgta
     1621 ggcgccggtg gcgcgggatt acgtgctgca attgctgccg cgcaggcaaa tccaaatgca
     1681 aaaatcgcac taatctcaaa agtatacccg atgcgtagcc ataccgttgc tgcagaaggg
     1741 ggctccgccg ctgtcgcgca ggatcatgac agcttcgaat atcactttca cgatacagta
     1801 gcgggtggcg actggttgtg tgagcaggat gtcgtggatt atttcgtcca ccactgccca
     1861 accgaaatga cccaactgga actgtgggga tgcccatgga gccgtcgccc ggatggtagc
     1921 gtcaacgtac gtcgcttcgg cggcatgaaa atcgagcgca cctggttcgc cgccgataag
     1981 accggcttcc atatgctgca cacgctgttc cagacctctc tgcaattccc gcagatccag
     2041 cgttttgacg aacatttcgt gctggatatt ctggttgatg atggtcatgt tcgcggcctg
     2101 gtagcaataa acatgatgga aggcacgttg gtgcagatcc gtgctaacgc ggtcgttatg
     2161 gctaccggcg gtgcgggtcg cgtttatcgt tacaacacca acggcggcat cgttaccggt
     2221 gacggtatgg gtatggcgct aagccacggc gttccgctgc gtgacatgga attcgttcag
     2281 tatcacccaa ccggtctgcc aggttccggt atcctgatga ccgaaggctg ccgtggtgaa
     2341 ggcggtattc tggtcaacaa aaatggctac cgttatctgc aagattacgg catgggcccg
     2401 gaaactccgc tgggcgagcc gaaaaacaaa tatatggaac tgggtccacg cgacaaagtt
     2461 tctcaggcct tctggcacga atggcgtaaa ggcaacacca tctccacgcc gcgtggtgat
     2521 gtggtttatc tcgacctgcg tcacctcggc gagaaaaaac tgcatgaacg tctgccgttc
     2581 atctgcgaac tggcgaaagc gtacgttggc gtcgatccgg ttaaagaacc gattccggta
     2641 cgtccgactg cacactacac catgggcggt atcgaaaccg atcagaactg tgaaacccgc
     2701 attaaaggtc tgttcgccgt gggtgaatgt tcctctgttg gtctgcacgg tgcaaaccgt
     2761 ctgggctcca actccctggc ggaactggtg gtcttcggcc gtctggccgg tgaacaagcg
     2821 acagagcgtg cagcaactgc cggtaatggc aacgaagcgg caattgaagc gcaggcagct
     2881 ggcgttgaac aacgtctgaa agatctagtt aaccaggatg gcggcgaaaa ctgggcgaag
     2941 atccgcgacg aaatgggcct ggcaatggaa gaaggttgcg gtatctaccg tacgccggaa
     3001 ctgatgcaga aaaccatcga caagctggca gagctgcagg aacgcttcaa gcgcgtgcgc
     3061 atcaccgaca cctccagcgt gttcaacacc gacctgctct acaccattga actgagccac
     3121 ggtctgaacg ttgctgaatg tatggcgcac tccgcaatgg cacgtaaaga gtcccgcggc
     3181 gcacaccagc gtctggacga aggttgcacc gagcgtgacg acgtcaactt tctcaaacac
     3241 accctcgcct tccgcgatgc tgatggcacg actcgcctgg agtacagcga cgtgaagatt
     3301 actacgctgc cgccagctaa acgcgtttac ggtggcgaag cggatgcagc cgataaggcg
     3361 gaagcagcca ataataagga gaaggcgaat ggctgagatg aaaaacctga aaattgaggt
     3421 ggtgcgctat aacccggaag tcgataccgc accgcatagc gcattctatg aagtgcctta
     3481 tgacgcaact acctcattac tggatgcgct gggctacatc aaagacaacc tggcaccgga
     3541 cctgagttac cgctggtcct gccgtatggc gatttgtggt tcctgcggca tgatggttaa
     3601 caacgtgcca aaactggcat gtaaaacctt cctgcgtgat tacaccgacg gtatgaaggt
     3661 tgaagcgtta gctaacttcc cgattgaacg cgatctggtg gtcgatatga cccacttcat
     3721 cgaaagtctg gaagcgatca aaccgtacat catcggcaac tcccgcaccg cggatcaggg
     3781 tactaacatc cagaccccgg cgcagatggc aaagtatcac cagttctccg gttgcatcaa
     3841 ctgtggtctg tgctacgccg cgtgcccgca gtttggcctg aacccagagt tcatcggtcc
     3901 ggctgccatt acgctggcgc atcgttataa cgaagatagc cgcgaccacg gtaagaagga
     3961 gcgtatggcg cagttgaaca gccagaacgg tgtatggagc tgtactttcg tgggctattg
     4021 ctccgaagtc tgcccgaaac acgtcgatcc ggctgcggcc attcagcagg gcaaagtaga
     4081 aagttcgaaa gactttctta tcgcgaccct gaaaccacgc taaggagtgc aacatgacga
     4141 ctaaacgtaa accgtatgta cggccaatga cgtccacctg gtggaaaaaa ttgccgtttt
     4201 atcgctttta catgctgcgc gaaggcacgg cggttccggc tgtgtggttc agcattgaac
     4261 tgattttcgc gctgtttgcc ctgaaaaatg gcccggaagc ctgggcggga ttcgtcgact
     4321 ttttacaaaa cccggttatc gtgatcatta acctgatcac tctgacggca gctctgctgc
     4381 acaccaaaac ctggtttgaa ctggcaccga aagcggccaa tatcattgta aaagacgaaa
     4441 aaatgggacc agagccaatt atcaaaagtc tctgggcggt aactgtggtt gccaccatcg
     4501 taatcctgtt tgttgccctg tactggtaag gagcctgaga tgattaatcc aaatccaaag
     4561 cgttctgacg aaccggtatt ctggggcctc ttcggggccg gtggtatgtg gagcgcaatt
     4621 attgcgccgg tgatgatcct gctggtgggt attctgctgc cactggggct gtttccgggt
     4681 gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga gcttcattgg tcgcgtattc
     4741 ctgttcctga tgatcgttct gccgctgtgg tgtggtttac accgtatgca ccacgcgatg
     4801 cacgatctga aaatccacgt gcctgcgggc aaatgggttt tctacggtct ggctgctatc
     4861 ctgacagttg tcacgctgat tggtgtcgtt acaatctaac gcatcgccaa tgtaaatccg
     4921 gcccgcctat ggcgggccgt tttgtatgga aaccagacct tatgttcaaa acgacgctct
     4981 gcgccttatt aattaccgcc tcttactcca catttgctgc ccctcaacaa atcaacgata
     5041 ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa gatccccggt atggcggtgg
     5101 cggtaattta tcagggtaaa ccttattact ttacctgggg ctatgcggac atcgccaaaa
     5161 agcagcccgt cacacagcaa acgttgtttg agttaggttc ggtcagcaaa acatttacgg
     5221 gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa gttaagcgat cccgcaacaa
     5281 aatactggcc tgaacttacc gctaaacagt ggaatgggat cacactatta catcttgcga
     5341 cctacaccgc tggcggcctg ccattgcagg tgccggatga agtgaaatcc tcaagcgact
     5401 tgctgcgctt ctatcaaaac tggcagcctg catgggctcc aggaacacaa cgtctgtatg
     5461 ccaactccag tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt ttgagttttg
     5521 agcaggcgat gcaaactcgt gtcttccagc cactcaaact caaccatacg tggattaatg
     5581 tacctccccc agaagaaaag aattacgcct ggggatatcg cgaaggtaag gcagtgcatg
     5641 tttcgccagg ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt gaagatatgg
     5701 cccgctgggt gcaaagcaat ttaccccccc ttgatatcaa tgagaaaacg cttcaacaag
     5761 ggatacaact ggcacaatct cgctactggc aaaccggcga tatgtatcag ggcctgggct
     5821 gggaaatgct ggactggccg gtcaatcctg acagcatcat taacggcagt gacaataaaa
     5881 ttgcactggc agcacgcccg gtaaaagcga ttaagccccc aactcctgca gtacgcgcat
     5941 catgggtaca taaaacgggg gcgaccggcg gatttggtag ctatgtcgcg tttattccag
     6001 aaaaagagct gggtatcgtg atgctggcta acaaaaacta tcccaatcca gcgagagtcg
     6061 ccgccgcctg tcagattctc aacgctctac agtaaaattt catcgggtcc gaattttcgg
     6121 accttttctg cgcttttcct tgctgtcatc tacacttaga aaaaaaccag taaggaaaca
     6181 tttatgcgcc tgctccctct cgttgccgca gcgacagctg catttctggt cgttgcctgt
     6241 agttttccta cgccgccgcg tggcgtgacc gtagtgaata atttcgacgc caaacgttat
     6301 ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg actggaaaaa
     6361 gtcaccgcaa catacagcct gcgtgatgac ggcggcctga atgtcattaa taaaggctat
     6421 aaccctgaca gaggaatgtg gcagcagagt gaagggaaag cgtactttac cggcgcacca
     6481 actcgcgctg cgctgaaagt gtcattcttt ggtcctttct atggcggtta taacgttatt
     6541 gcactcgatc gggaataccg ccatgcgctg gtttgcggcc cggaccgcga ctacctgtgg
     6601 atactctccc gcacgccaac catttctgac gaagtgaaac aggagatgct ggcagtcgcg
     6661 acccgggaag ggtttgatgt cagtaagttt atttgggtac agcagcccgg tagttagtga
     6721 gtgctgagtt tcagaccaat aatccccaat acgattaacg ccagactcgc cagacgcatc
     6781 gggttagcgg actcaccgag cagcacaatg ccggtgatgg ccgcgccgac tgcgccaata
     6841 cccgtccaca cggcataagc cgtccctacc ggtaacgatt tcatcgccca ggcaagtagc
     6901 gccatactga ctatcatcgc cgtcacggta ataacactcg gcgtcaaacg gctaaagccg
     6961 tgggtatatt tcaggccaac ggcccatacc acttccagca gaccagcaat aactaagata
     7021 atccaggaca tatcaggctc cggaaatttg gggccgtccc cggtgaaaag aggcgtttgc
     7081 aggtcgtcct gcaaagctga tggttgaacg gatattttgc caggtggcag gggaatttca
     7141 actattacct ttatgacgat cgcagaaaaa acaaacggca cgacactgct gtcgtaccgt
     7201 tattgcttat tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat
     7261 gtcttcacca acgccacgcg tggtgttgca ggcagttaat actgttgaaa gcaccagaac
     7321 agaaaagatc gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt
     7381 tatgtttgcc tatagcaaat taataataga caacatcgcc aaaaatgatg gaaatctgat
     7441 gatttttcgg aagagaagaa aaattagctg gcagcgcgag agatggagtt accgagatgt
     7501 ttgatgtctt cgccgaaacc gcaagcggtg ttacagcccg tgagcagcgt gctggcaagc
     7561 aaaacaagaa cgataaggcg tttcatcatc tctgacctga ataagtgatg gtgcagcctg
     7621 caggccgcac cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc
     7681 actttgatga cttcgccgat ttgtacgaac agcggaactt taaccacagc gccagtagac
     7741 agggtagccg gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatca
     7801 acgatttcca gttcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga
     7861 gttacgatac actctgcctg atccagcagc catttagcat tgtcgccaat tgcttttgca
     7921 tcagcagaca gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg
     7981 tacaggtaag tcaggttcat atcgacaaca tcagcgcctt cagcggaatc agtagatttg
     8041 aaggtttttt ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc
     8101 tggcctttac ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac
     8161 atgattttaa gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa
     8221 atttgttaat tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc
     8281 cagagaagat tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg
     8341 tcttttgaat atagacgcgg acgaaaaact gttggccgga cgcagcgcca aaaagctgtt
     8401 tgccctgcgt gtgccccgct catttatcga tcgcatggag aaaggcaatc cggacgatcc
     8461 tcttttgcgt caggtactta cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac
     8521 cgacccactg gaagaacagc acagcgtagt gcctggtttg ttgcataaat accacaaccg
     8581 ggcgcttttg ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca
     8641 cttcccctat gccgaaaatc agggcaacaa gcgtaactgg cagactgcac ttgagtatgt
     8701 tgctgcgcat ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa
     8761 agagcacgag ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct
     8821 gcggattcac agccgtctgc cgattgtgat cccggcacgt atcaccgagg cgctggttga
     8881 acgctttgcc cgttctacgc tgcaaatctt gctggtgaat cacatcaatc atgccaatga
     8941 ggtagatgaa acgttccgtc aggcgatggc taaattgcgc cgtgtcggtg tcaccctgct
     9001 taaccagagc gttctgttac gtggtgtgaa cgataacgca caaacgttgg caaacctgag
     9061 taatgcgttg ttcgatgccg gcgtgatgcc gtattacctg catgtgctag ataaagtaca
     9121 gggcgcggcg cattttatgg tgagtgatga cgaagcacgg cagattatgc gtgagttgct
     9181 gacattggtg tcgggatatc tggtgccgaa actggcgcga gaaattggcg gcgaacccag
     9241 caaaacgccg ctggatctcc agctacgcca gcagtaactg attaaacagg gaagttaaac
     9301 attaaaattg cctgatgcgc tacgcttatc aggcctacat cgtgcattga aattgaatgg
     9361 atttgcaatg aactgtaggc cggataaggc gtttacgccg catccggcaa gagcaacaaa
     9421 cgcaagtaca gcaaccactg agaaatttaa tctttgcacg ttgcataacc tttaattatt
     9481 catatgcatt gctgttgtta ctttttatat agctaacacc aataattaac ctttct
//
LOCUS       DAJTPG010000131         9497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTPG010000131 DAJTPG010000000
VERSION     DAJTPG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..200)
                     /locus_tag="OO230_003502"
     CDS             complement(<1..200)
                     /locus_tag="OO230_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693128.1"
                     /translation="MCYEYDAYYLYRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGV
                     VCDPYICVNSDGISPELTRKYLG"
     gene            713..1408
                     /gene="minC"
                     /locus_tag="OO230_003503"
     CDS             713..1408
                     /gene="minC"
                     /locus_tag="OO230_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5693129.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1432..2244
                     /gene="minD"
                     /locus_tag="OO230_003504"
     CDS             1432..2244
                     /gene="minD"
                     /locus_tag="OO230_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5693130.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2248..2514
                     /gene="minE"
                     /locus_tag="OO230_003505"
     CDS             2248..2514
                     /gene="minE"
                     /locus_tag="OO230_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5693131.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2886..3230)
                     /locus_tag="OO230_003506"
                     /pseudo
     CDS             complement(2886..3230)
                     /locus_tag="OO230_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3265..3384)
                     /locus_tag="OO230_003507"
     CDS             complement(3265..3384)
                     /locus_tag="OO230_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5693132.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3345..3530)
                     /locus_tag="OO230_003508"
     CDS             complement(3345..3530)
                     /locus_tag="OO230_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693133.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3631..3804
                     /locus_tag="OO230_003509"
     CDS             3631..3804
                     /locus_tag="OO230_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693134.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3806..4150
                     /locus_tag="OO230_003510"
     CDS             3806..4150
                     /locus_tag="OO230_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5693135.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4154..4489
                     /locus_tag="OO230_003511"
     CDS             4154..4489
                     /locus_tag="OO230_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5693136.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4546..6867)
                     /locus_tag="OO230_003512"
     CDS             complement(4546..6867)
                     /locus_tag="OO230_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5693137.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6984..7193)
                     /locus_tag="OO230_003513"
     CDS             complement(6984..7193)
                     /locus_tag="OO230_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693138.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7944..9467)
                     /locus_tag="OO230_003514"
     CDS             complement(7944..9467)
                     /locus_tag="OO230_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5693139.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2666 a         2205 c         2078 g         2548 t
ORIGIN      
        1 ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca aatatacggg
       61 tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc agacgccatt
      121 gatgagaaca tcatccccac acctgacaat ccgatataaa taatccgata taaataatac
      181 gcatcatatt catagcacat cctgataatc caaccgtaca acacttatgc tcatttcgtc
      241 atcaatccgt aatcaaaacc gctgacctgg acgagttacg atgacctgac agaagtatca
      301 gacgtcttct gcggtttcta tgatagagca attctgtggc ggatattaag ataacgggta
      361 tctgttcttt aatgatgctg caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa
      421 atacacttgc ctcaatactg gatatttaaa caggtctgga gatgctgtca cgattagggt
      481 gcggaaaaag gaagcgtaaa taagaagaat caaaagtacg acggcaatgg gttgattgac
      541 aagggtattt tttaagctat gaatcagcgc catttatcac agaatagact tttactctga
      601 ataaatggga gggtgacttg cctcaatata atccagacta taacatgcct tatagtcttc
      661 ggaacatcat cgcgcgctgg cgatgattaa tagctaattg agtaaggcca ggatgtcaaa
      721 cactccaatc gagcttaaag gcagtagctt cactttatct gtggttcatc tgcatgaggc
      781 agaacctaag gttatccatc aggcgctgaa agacaaaatc gctcaggccc ccacattttt
      841 aaaacatgcc cccgttgtac tcaacgtcag tgcactggaa gacccggtaa actggtcagc
      901 gatgcataag gcggtttcgg caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga
      961 tgcgcaactt aaagccgaaa ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga
     1021 aaaagcgcca cgtccagctc ccgcaccgca ggctccagcg caaaatacaa tgccggtcac
     1081 aaaaacgcgt ttaatagata ccccggtgcg ttccggtcag cgtatttatg ctccacaatg
     1141 tgatctgatt gttacaagcc acgttagcgc tggggccgaa ttgattgccg atgggaacat
     1201 tcatgtctat ggcatgatgc gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac
     1261 gcaaatattt tgtacgaacc tgatggcgga attggtgtcc atcgcaggtg aatactggct
     1321 gagtgatcaa atcccagcag aattttatgg caaagcggcg cgactgcagt tagtcgaaaa
     1381 cgctttgacc gttcaaccgt taaattgatc cctttttaac aaggaatttc tatggcacgc
     1441 attattgttg ttacttcggg taaagggggt gttggtaaga caacctccag cgcggccatc
     1501 gccactggtt tggcccagaa gggaaagaaa actgtcgtga tagattttga tatcggcctg
     1561 cgtaatctcg acctgattat gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc
     1621 attcagggcg atgcaacgct aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc
     1681 tatattcttc cggcatcgca aacacgcgat aaagatgccc tcacccgtga aggggtcgcc
     1741 aaagttcttg atgatctgaa agcgatggat tttgaattta tcgtttgtga ctccccggca
     1801 gggattgaaa ccggtgcgtt aatggcactc tattttgcag acgaagccat tattaccacc
     1861 aacccggaag tctcctcagt acgcgactct gaccgtattt taggcattct ggcgtcaaaa
     1921 tcacgccgcg cagaaaatgg cgaagagcct attaaagagc acctgctgtt aacgcgctat
     1981 aacccaggcc gcgtaagcag aggtgacatg ctgagcatgg aagatgtgct ggagatcctg
     2041 cgcatcaaac tcgtcggcgt gatcccagag gatcaatcag tattgcgcgc ctctaaccag
     2101 ggtgaaccgg tcattctcga cattaacgcc gatgcgggta aagcctacgc agataccgta
     2161 gaacgtctgt tgggagaaga acgtcctttc cgcttcattg aagaagagaa gaaaggcttc
     2221 ctcaaacgct tgttcggagg ataagttatg gcattactcg atttctttct ctcgcggaag
     2281 aagaacacag ccaacattgc aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc
     2341 agcgatgcag aaccgcatta tctgccgcag ttgcgtaaag atattcttga ggtcatttgt
     2401 aaatacgtac aaattgatcc tgagatggta accgtacagc ttgagcaaaa agatggcgat
     2461 atttctattc ttgagctgaa cgtgacctta ccggaagcag aagagctgaa ataagcccgc
     2521 tgtaaaaacg catttatctt caaggcagag tcatttctgc cttgagtttt tcatccctct
     2581 catccacgtt gtggtaaagc ggcgagtatt cttgctgata ctcctcattg ctatttccac
     2641 ctcccccttt acaccttaag gctgttccca cgcacagaac aaaatgttta tgcctggttg
     2701 agtaaataac catgttatta gtgtgtatat actgggagat gataaccccg caatttcatt
     2761 attcattgtt ctcccatagg atgaggtttc ccgccgtaac tggcgggctt tttttgcccc
     2821 agaattttct ctttcaacat cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt
     2881 gatggtcaga acgaccagtt cacaccagcc accgcgttcc acggggactc cacaccggca
     2941 ccatggctat accccacccc gaaatgcccg cttaacgtac tgctgaatga ggctttaata
     3001 cctgcctggt atattccacg tctgcccgac aaatcattga caaaattacc gtcactattc
     3061 actttcaccc gattatcatc gacaaattct ttgcgcacag ccgccttcag gcagggttca
     3121 atttccatac cgttccccag acgcatgttg taactcagcg ttgcacccag ttcacgatat
     3181 atactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata gtagcgtcta
     3241 agtgacctaa gctctgctgc aacatcagcc cgcaggcacc agaccagggg attcatcctg
     3301 aagagacagc gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg
     3361 atatcccgca taagaaagag gcatttttta cacgcagtgc gctgaagtgt agttggataa
     3421 aaaatcactc ttttcagata atactgtcgt acgcttatct ttagacaatg aggccgtcgg
     3481 aaaaataaaa tgactaatgt aatcgttgct gatgaatatc ttctgctcac ataaaaatca
     3541 cacaataata ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg
     3601 acacttcttg aataaaaata ttgtcaatca atgagttatt cctcatttaa gattattctc
     3661 attcacgtaa aaaacataat tcctatcatc acagccacgc tcattttgaa ttatctgaat
     3721 aatagcgagc gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca
     3781 acttacttaa taccggacat ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc
     3841 agcattgttc tgctccacgc cagcgatggc ggatatgaac cgcaccacga aaggtgcatt
     3901 gctaggcgca ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc
     3961 agctgttggc gctggtgtta gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg
     4021 taaaggtgca aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg
     4081 cctcgaaggg gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa
     4141 aatgaaataa ggaatgatga tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc
     4201 accaatgacc tgggcgcagg actacaatat taaaaacggt ttgccgtcag aaacctatat
     4261 tacttgcgca gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt
     4321 tgcggtaatg ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga
     4381 actgagcgcc aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct
     4441 gttgatcacg gcaattgatg acaccatgcg tgctatcggc aaaaaataat atttcatatg
     4501 taccgcgtcg atgaatacga cgcggttttt tagctaacgg cgggatcaga acttatattt
     4561 caggcccacc atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt
     4621 cccccagacg ctggccgctg gattcaggtt gccatttacg ccaagtttaa tttcccccaa
     4681 attactcacc ccatcttgtt cgactttcac accgttcatt gagacagcaa aatctttact
     4741 gttatggatc cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt
     4801 tttctcctga gtacgaataa aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc
     4861 gtcattctca atacgtgagc cattggcttc cgtgtgcgca ttatgatcga cgcccatcca
     4921 gatagcctgt gcctgtggct ggaagatcca cgtatatttc gccgcttcca gaccaaagga
     4981 tttattcagg gcaaaaccgt aacccgcttc cagcgagacg gttgcacctt tcgcgctata
     5041 ggattcatag gctaattcat cccctttcac ggagttttta aaccagctat attgcgccca
     5101 ggagtcaatg tatgcgcctt ttttactgat gtcatcggca aaccaagtgg catacagccc
     5161 tgcgctatag cctctgacac tccctttcga acgataatcc gacacgctgg aatgagttaa
     5221 gttgtagtcg cgggcataac cagccatcac tcccaaacgc caactgtcac tatcggtaaa
     5281 accaccggtt aacagatcgc cccctaactg cgagacgtag cgatgcgagg tcgttctcaa
     5341 ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaagtcggct
     5401 tgaacgctcc tgttcagtta caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag
     5461 acgcataaca aaaagagtgt tagcggctgc caggttggcg acgtaacttc cagcttcggg
     5521 gcgcaggacg ggggtaggcg caacagtagg cgctgaactt cctccggagt ttgtttcacc
     5581 gccgctcgtt gcctggaagt tagtcagata ccagttttta ttggtcgctt cggtattacc
     5641 tcgggttaat gaatattcgt aggcaccggc gaaaatcctc gaatccttaa taaattcccc
     5701 atttgattcc ccctcaacgc tgataatctc tatcccattt aacgtgtaag ctccgctgcc
     5761 tcctgcattg gtaactttaa ctcgggttgt tccagaggta tttccattaa taacgacttt
     5821 atccgtagct gaattatcat catccaattc agttctgaga tgcaatacgc cattattacc
     5881 aacgtaattt tcagttattg ttaatcttgt tggttcgaca tcccgtccat ctgcccggga
     5941 aatatagacc gtactattat caaccgtgag gttgctcacc gttgagtcag ctttaatatc
     6001 ccaggtacta ttatctgaaa gcgacagata ggtatgagtg ttatcatctg ttgaaatatt
     6061 agctgaaccc gttatttttg aattagttgc atcgacggtc aaatgacctt taatattatt
     6121 gataaaaaca tctgcagccg atgatacctc actatccgta atggtcacgt tactcgtctc
     6181 atctttatgg tagaggattt catttgctga ccgtagtgag ctaccagata aaatatctac
     6241 cgttgcttcc tgaccattta tggaagactc cagcacaatt ccagacccct gatgcgcgac
     6301 tgcagatgta tttttcagcg tcaccgtgcc gccattataa attttagcaa tcgcggcgtc
     6361 aggacccgtt gtataaattt ctccctggtt aaatgaaact gtcccctggc gggcagcgat
     6421 ggcatgagca ttgtccccat gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt
     6481 ggtataatca agcgtgtcac cagccttgac gacagtgcca ttagcgtacg cggcataact
     6541 ttctgttccg tgggttgtga tctccccttt tgccatatcc agttttgcta tggcattatt
     6601 ttcattactt cctgtatcgg cagtactttc ttcatcaaga gaagatacac acgcacacgc
     6661 gcttccggga gatgcacacg caagaacgcc gactgcgtga ttacccgcag taacaataaa
     6721 tgtatcatct ccgtcagaaa aagagggggt aaccgaaccg tcagagttga ctcttattat
     6781 ggtgtcagta ttcaggtttc cactcgtttg acttagcaaa ccaattgagt aatcccccgt
     6841 tgtgacaatc gtgtttttac cctgcaaaat aacagcaccg ccaaattcac ttacgattgc
     6901 gctagctgtt aatgctgggc ttagaggact attgtcagaa ggatataaac cgcccgttgt
     6961 tacaatgacg ccattgttaa tggttaactt actaccctct cctgcaacgg atacccgtaa
     7021 cttccattac ccgttgttat aactagcgca ttatcaatat cttttacttt tccatctttt
     7081 attgccacag catgtttata ttcatcgtgc tgaataaact gttcagcata tgcattaccc
     7141 accgccagag caataagtcc caaactaaac ccggggagtt ttttcagttt catagattaa
     7201 ctatccattc attggattaa caaacattcc tggtcaaata acctatgagc tcagattacc
     7261 aaaacggagg gtaaagacaa aaacaaaagc acaaaaaatt taaatagata aacagcgaca
     7321 atatataaat gataaataag tcggcattat catttttcac cttattttca tgacattgat
     7381 cactttgggg tgactatttt gtttaacaaa taactcttcg acatcaaaat tattaatagt
     7441 tgccatggta attatttcgg tgatatataa aaacaactgt gataacacca aggttgacaa
     7501 ctatttgcgc aataattcct ttccttcaat gatccaggcg ttcactgcca gcaacccatt
     7561 cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg gctaacgagt
     7621 ctttctctta aatcgattaa ccagattgat cgccagccaa acaagggcat taagcaaatt
     7681 tatcgtcaca agcacttctc ccagcccggt actgggtttt gcaataagga tgaccaggag
     7741 ggtgatcagc ataaatgcaa tggaaaagat gatatagaga gtgaaataat ccagattatt
     7801 actattattc atgctactaa tggcaatcct gtcaaaactt tcaaacaatt atagcaaaca
     7861 aaataatgaa caatagtaac aagaggcaac gagctaatta gctgtctata attacaccgc
     7921 actatggcct tactgatcaa ttttcactca accacaacct tcaccttcgg tttagaaagg
     7981 ataattttga ccaggtcgat gtatgtaaca ggtttataga aataataacc ctgctgaaat
     8041 gtgatattat tttggttcag atagccaagt tgttctttcg tttcgacacc ttcagcgacg
     8101 atactgatcg aaagtttacg cgccagatcc aatacacaat ccagaattcg ggttgattct
     8161 gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt tgatataatc aatatgcagg
     8221 tcatgaagat aagaaagccc tgagtagcca gtaccgaaat catccagcgc gatgacaaaa
     8281 ccattttcat gcagaatgtt caaccgctga accagacttt catcaacatt caatggctca
     8341 cgctcggtga cttccagaac aaggtttaaa tcgcggcggg taaaactgtc acggaaattt
     8401 aaacactcgt cgacaaacgt cggcgaaata atatgcgaag cgctaaaatt aattccaata
     8461 tgaaaacctt ccggcagttt actcgcgata gcgttcatct gtctggcaac ctgattcatc
     8521 aggctttgtg taagcggaac gattaatccg gatttttcag caagtggaat aaatgccgcg
     8581 ggtgaaatat atccaccgtg aggttgtttc cagcgggcta acacctcaac tccccgcaat
     8641 gtcccttccc gaccatttac cacaggttgg taaaaaggga cgatctcccc attatttatt
     8701 gcccgtcgca ggatctcttc aggcgttgcg cttttattga aatatctatc aagcaggaag
     8761 gcagcggcgc tggcgattaa cagaataaat attaaaatcc caaagccttt atcgataagt
     8821 ctgtagaagc taaagagtgg cggcgggtta aagataatag tgaaatgata ttttgtggag
     8881 tgaaccttct cggtataggg ataatgtcca ttaagcgtca tcacatcagc cgttggtcca
     8941 ataatgtcat ccgccacgcg taatacatac cttacccctt tcaagggtac attaagcgcc
     9001 ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg tatctgcaaa ttggctctga
     9061 tagagcagga taggtaatct attaacggtg tcgattgccg gagccaacag taaattactg
     9121 tccgggacaa caggaatacg agacagcagg acccgattcc caggcaggga tgtacaccag
     9181 actattccct gtttgagaat gatgattgtg cgcaggtgag gtttcagagc tgcttcagtg
     9241 ccaagctgat attgctcctc gaggtcgcat tccttcccgg caatgttaac cgctgtttga
     9301 gtcgcctgtg acgcttcttc aagtatatga ttgatattgt tggcggcata tcttgctccc
     9361 gccaggtact ctgcttttgc ggaataccag agctggatgt tcaaaatagc gacaccggta
     9421 ataaacaagc atatcgcaac gaggatgggt atgagtgtat tgcgcatttg cttgtcccgg
     9481 ccaaagggtt taataca
//
LOCUS       DAJTPG010000132         9457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTPG010000132 DAJTPG010000000
VERSION     DAJTPG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="OO230_003515"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="OO230_003515"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5693140.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO230_003516"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO230_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5693141.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO230_003517"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO230_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5693142.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="OO230_003518"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="OO230_003518"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5693143.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="OO230_003519"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="OO230_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="OO230_003520"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="OO230_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5693144.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="OO230_003521"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="OO230_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5693145.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7297..7566)
                     /locus_tag="OO230_003522"
     CDS             complement(7297..7566)
                     /locus_tag="OO230_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5693146.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7623..8291
                     /locus_tag="OO230_003523"
     CDS             7623..8291
                     /locus_tag="OO230_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5693147.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8607..>9457
                     /locus_tag="OO230_003524"
     CDS             8607..>9457
                     /locus_tag="OO230_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5693148.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEE"
BASE COUNT         2719 a         2137 c         1932 g         2669 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actttggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta
     7201 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt
     7261 cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg
     7321 ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt
     7381 ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg
     7441 aagtggcgca atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca
     7501 gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
     7561 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
     7621 atatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
     7681 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
     7741 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
     7801 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
     7861 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
     7921 aacataaaat tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg
     7981 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
     8041 gtgtaatacg caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa
     8101 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
     8161 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     8221 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     8281 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta
     8341 cgatttgggg ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat
     8401 gaacgtacga tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat
     8461 ctaagaaaat agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac
     8521 aataaatttc aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta
     8581 tcaggatatc cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg
     8641 gaaacgtctc tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg
     8701 aatgggttga atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc
     8761 ggctccaatt atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca
     8821 gatctctacc acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat
     8881 caatgctccc cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat
     8941 ctgcacttcc tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt
     9001 tcgcacttcc tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt
     9061 ctgtacttcc gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg
     9121 aagtaatacc agattttcca gaaagagatg atcatattat aagaatattc tggcttaatc
     9181 agaaccggat cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca
     9241 atcttagaga aaatcaacta tctcccagaa taatgcaaac tttgttacaa caaaccgccc
     9301 aaccggacta ccacggccca cggatttact tctccatgag tgacggacaa cagaatacac
     9361 tccatcgccc cctggctgat gccgtgacag catggttccc ggaaaacaaa caatctgatg
     9421 tatcacagat atggcatgct tttgaacatg aagagca
//
LOCUS       DAJTPG010000133         9331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTPG010000133 DAJTPG010000000
VERSION     DAJTPG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(139..1041)
                     /locus_tag="OO230_003525"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="OO230_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="OO230_003526"
     CDS             1101..1379
                     /locus_tag="OO230_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693149.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="OO230_003527"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="OO230_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5693150.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="OO230_003528"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="OO230_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5693151.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="OO230_003529"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="OO230_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5693152.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="OO230_003530"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="OO230_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5693153.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..5017
                     /locus_tag="OO230_003531"
                     /pseudo
     CDS             4371..5017
                     /locus_tag="OO230_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5059..5589)
                     /locus_tag="OO230_003532"
     CDS             complement(5059..5589)
                     /locus_tag="OO230_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5693154.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5745..6311)
                     /gene="slp"
                     /locus_tag="OO230_003533"
     CDS             complement(5745..6311)
                     /gene="slp"
                     /locus_tag="OO230_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5693155.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6558..7781)
                     /locus_tag="OO230_003534"
     CDS             complement(6558..7781)
                     /locus_tag="OO230_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693156.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8362..>9331)
                     /locus_tag="OO230_003535"
     CDS             complement(8362..>9331)
                     /locus_tag="OO230_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5693157.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2719 a         1858 c         1941 g         2813 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gttggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggttacgca cacatgtatt aattggcatg ggaagcgccc
     4501 tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac
     4561 tcgaccccag ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta
     4621 acattctcgt tcgtaaccaa aatattgtag gtctgacgac ggcagcggat atctgggtga
     4681 ccgccgcata ggtatggtta ttggcagcgg tatgtacgaa ctgggtattt atggttcagt
     4741 gatgaccttg ttggtgctgg aagtcttcca tcaattaacc ttccgcctga tgaacaaaaa
     4801 ttaccattta cagctgacat tagtgaacgg taatacggta tcaatgctcg actggtttaa
     4861 gcagcaaaaa ataaaaactg atttagtttc gttgcaggaa aatgaagatc atgaagtggt
     4921 cgccattgat atccagcttc atgccacaac gttgatagaa gacctgttgc gattattaaa
     4981 aggtatggca ggggtgaaag gcgtttcaat tagctaatct gtgtggcagc gtagccagac
     5041 tcaccgtaag cctgaaattc acaccagata atcaatatgc tgatggcgta ccagctcatt
     5101 gatccgctta agattgagga tcatcatgat attgtgttta tggcagtaga acgtttttag
     5161 cgatattttc agtttctcca gaatatcttc agttgtcatt tgattcgcaa tgcagctcag
     5221 aacctgatgt tgcttgcgcg aaagtgacac attcgcttca ggcacagagc agtaactgcc
     5281 attgatgagt gcgacaaaat caatgatagt gatagtccgc ggaacaaagg tcaccggtac
     5341 tttccccaga cagcgtttaa tattaaaggg ggagaaaaca atgacatgga caggttttaa
     5401 tcgttcaatt tgagtgagaa aattagaatg aaatacatta ttcattaatg tatcaataat
     5461 gacgaaggca ttctgatgca acattacgtc gatctcttct tcgttctgta tatgaacgac
     5521 attaccttta ctcagaatgt ttttcatcgc ggtgaagaac atcgtatccc tggtaattat
     5581 aagaaacata accgactcct ggtgcggaca ttaactttat aatcagtttg ccaaaggtaa
     5641 ttaacaggac agggcttata ataataatag ttagcttact gtatattaat tttatgtaat
     5701 acatgatgca gatgctgaga aagcatctgc atctttcggt ggtgttattt gaccagctca
     5761 ggtgttacct gactcaccgc attggtgtag taaggcgcac cccagcccgg ttccggccag
     5821 aatgcaccat agccgtaatc ccacaggtta taggtggtat taaccacttc tctcaaatgc
     5881 cacacctgga tgccctgcat attcacttcc aggaagttat acgggacttt attgataaag
     5941 cctggttgtt caccctgaat ggtaccgagg atggtaacaa agtgattacg atagttcact
     6001 ggatcaagga agccgctttg tctggcgagc agtcggccct gatagttggc ttcaatatca
     6061 ggcttcgcat agctatccaa cggtaataca gcgatttcta acaacgtatc cgttttgcca
     6121 ttgataacgt tgataacctt cccaccaaag cgcgcttgtt gaccaacata taaccccggc
     6181 tggttatgaa cagcaacaaa acttttttga atatcaggtt ggttattgcc tttgatattt
     6241 tgcggaattg aactacatgc ggcaagcaaa aatgaaaggc tgaggatgag tgcacctttt
     6301 gtcatgttca tgttactatc cttatcaaca aatattaaaa ccataataga tatttcttta
     6361 tagttttcat ctgattctga ggtgatatgc ctgatagtaa cgtaaacctg tctgatgttt
     6421 atatcccttc tgtttagttc aattccgtta ttttggggcg tggtatgagt atccatcgcc
     6481 caataatata accctttgtt ttaaaattac ataaaatcta tttttattca atgattataa
     6541 agtgctaata atcaatatta tggtattata ttctgataat gatttgataa gcgaattctt
     6601 ttgccagatg gtatttctat ttccacccca taatttatat ctttttcatt aggcagatct
     6661 ctctttaaat aaaaggggta tgaaatcttt tcgggttgaa gtgtctcgta atgaaattct
     6721 atgaattcat ttttagacat tgttgcccat ttttgaattt tattgtatag agagggtgtt
     6781 gtaaactctt gaagttgttt ataacatatt tttttgaact cttgcttata ttttaatatg
     6841 tcatcaaatg aaagattcac tacctttgcc tctaagaaat caattattct cgaagcaatt
     6901 tccgctttcg cgcgtaaaga gttgtcatta atagttaaca atgtagaaaa caaaccattg
     6961 atttgtttgt caagatgttg ttcatattta tgtagtttgg ggatgggatt ttccttgtgt
     7021 gaaaaaatag tgttaaataa atttgcgtca gtgaaattta tattaagtag atcttcaaaa
     7081 taaaattttg ataaggttag gactagcttg gacatatctt gccctgtcat gttgaaatca
     7141 ataaagtcgc atccagcaag ggcttccatc ctattttcat ttttactgtg ctcaatatac
     7201 agcttcgcca tttttaagta caagctttga atgtctctta tatcagttgg taagtcagct
     7261 cgttgcccag caaaattttt attatcggaa gaaacggttt ggacactgaa tctttcgggt
     7321 attccttctt ctacgagaat tttagttatg attggattgt tgggttctgg attaacgaca
     7381 acaatgattt gacatacaga atccttatcc ttttcgtatc taatgttatg aatctgatta
     7441 agtattgatt gtgtctgttg cgtaagtggt gtcgaactta tcgtttcaat gttattaata
     7501 agacttacaa gttgaaccac aacttttgag tagtctgcat ttgtatatgt ggtgttcgtg
     7561 tggcatgcat taatttgttg gagttgttgt tgcgctaaat caaattgttc agggttgtat
     7621 ccttctccag agaatgtgga attaatgggt tgttgttctt cccaattatc acaaggccac
     7681 ttcggtgtgt ctggcgtttt aacattatcg gtatttgttg tgcagttata aatatcgccg
     7741 tgatatgtat ttattatacc tggggtaaag tcaattgaca taacattcct tattcatcat
     7801 ctgttttatg ctaatgtttt taaattggat aattagaaca ggaatggatt ttcttctaca
     7861 cacaataaat ataatgcgtt atatcttcct gatataatta cctttaaatt ccctttcatt
     7921 tccacccggt aatatttgtg tataaaaaaa gggcagtgat agtatcgtaa aggaaaacgt
     7981 taacaactat caaaaaacgc tcattttaaa aagttattat gttctccgat agtgtgtgaa
     8041 gtaatatttt tgtgatgttt tagatgtgta ataatgaacc aggttaaagt gtatttaaaa
     8101 atgtgttgta ttagcgactg aaatctgcct tttaaaaagg aggagggtac tgagacaaag
     8161 agtcattgct tctccgataa aatgtcagag aagcatgatt acgtggttat tgtctgttga
     8221 tgacttgttg accgagccgt catcattctc aatgcattag ttaaatcatt tgtgattttt
     8281 aaggattatc ctgtctatga ttttctactt agaaggatat ggcactaact atgcatctta
     8341 tagtttactg ctggcatcta tttaaaatag caattgagtc aaatagccca tcaagatagc
     8401 catacctaat ataaccgtca ggaatgcagc tatcatcggc attctgaaca ttgatttaag
     8461 taatatcact tccgtcaggc ttgcgccggc actgccgatg attaatgcca ttaatgctcc
     8521 cagacccatt ccttttgtca tcagaacaga tgccagaggg ataaccgcct cagcccggat
     8581 atacagcgga ataccgacaa cggcgcttaa tgggatggcg aagggattat ctgctcctgc
     8641 atgagcggca atccacgctg agggaataaa gccataaata aaagatccta ttaatactcc
     8701 taacaaaaga tatggcagta catctttaaa ctgttgcaac gcatctttgg tggctagttt
     8761 tatgggatta atacttacta cagctttggt actgcaacag gtaactacgg ggttctcaat
     8821 ggctttagcc gctggactac aacaactcac ttttatgggc gtatatgtcg ttcccggcga
     8881 agtttttgct ggagcacaac aatttgctga tgagctttta ctggcaatga tatgacgttc
     8941 aaaccccaga gaatccagga taatactggc aagaacggag acgccggctg cgataatcgc
     9001 gtacaacaag gtgactttcc aaccaaaggt catccacatt agtccgacga taatgggatt
     9061 aagtaatggt gaaacaaata aaaaagtgag agtcggacca aacccggctt tcgctgacaa
     9121 caatccacgt aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag
     9181 agcagccagg agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc
     9241 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
     9301 ctcgacggcg agaaacacaa acatctctgc g
//
LOCUS       DAJTPG010000134         9125 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTPG010000134 DAJTPG010000000
VERSION     DAJTPG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..554)
                     /gene="pflA"
                     /locus_tag="OO230_003536"
     CDS             complement(<1..554)
                     /gene="pflA"
                     /locus_tag="OO230_003536"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5693158.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLG"
     gene            complement(746..3028)
                     /gene="pflB"
                     /locus_tag="OO230_003537"
     CDS             complement(746..3028)
                     /gene="pflB"
                     /locus_tag="OO230_003537"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5693159.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3083..3940)
                     /gene="focA"
                     /locus_tag="OO230_003538"
     CDS             complement(3083..3940)
                     /gene="focA"
                     /locus_tag="OO230_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5693160.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4346..6106)
                     /locus_tag="OO230_003539"
     CDS             complement(4346..6106)
                     /locus_tag="OO230_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5693161.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6236..6862
                     /locus_tag="OO230_003540"
                     /pseudo
     CDS             6236..6862
                     /locus_tag="OO230_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7061..8149
                     /gene="serC"
                     /locus_tag="OO230_003541"
     CDS             7061..8149
                     /gene="serC"
                     /locus_tag="OO230_003541"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5693162.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8220..>9125
                     /gene="aroA"
                     /locus_tag="OO230_003542"
     CDS             8220..>9125
                     /gene="aroA"
                     /locus_tag="OO230_003542"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5693163.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELN"
BASE COUNT         2307 a         2190 c         2296 g         2332 t
ORIGIN      
        1 ccgaggcgat gtgccgaatc gtcatcgtca gaccagcctg ggacaacaac gtagcggatc
       61 cacaccttca catttttgtt cgccagatat ttagcgaact ccagcgtgcg gtggttggaa
      121 actccaacca gattttggtg gatctcgtcg ttcatctgtt tgagatcgag cattaccagg
      181 tcggttactt ccagcagttc atcaatcacc ggatcgtaac gacgaacaaa accgttggta
      241 tccagacagg tatgaatgcc ttctttttta caggcgcgga accagtcacg aacaaactca
      301 gcttgcagga ttgcttcacc gccggatgcg gtaacgccgc cgccggaagc gttcataaag
      361 tggcgatagg tcaccacttc cttcatcaaa tcttcaacgg taacttcttt accgccatgc
      421 gtgtcccagg tgtcgcggtt atgacaatac aggcagcgca tcaggcagcc ctggaaaaag
      481 gtgataaagc gaatacctgg gccgtctacg gttccacagg attcaaagga gtgaatgcga
      541 ccaataactg acattgcggt gtttctccag atgtggccca tctgaggccg tgttggtgcg
      601 cagctcgaag gctacgtcga gtctgttttg gcagtcacct taaagtatag atagctgaca
      661 aaaaaggctc tcgtgctaaa aaaggcccca ctttcgtgga gcctttattg tacgcttttt
      721 actgtacgat ttcagtcaaa tctaattaca tagattgagt gaaggtacga gtaataacgt
      781 cctgctgctg ttctttagtc agcgagttga aacgtactgc gtagccagat acacggatgg
      841 tcagctgcgg atatttttcc gggttttcca tcgcgtcgag cagcatttca cggttcatca
      901 cgttaacgtt caggtgctga ccaccttcga tggatgcttc gtggtggaag taaccatcca
      961 tcagaccagc caggttggtc ttacgaactt cgtcgtcttt acccagtgcg ttcggaacga
     1021 tagagaaggt gtaggagata ccatctttag cgtaagcaaa cggcagttta gcaacggaag
     1081 tcagagacgc tacagcacct ttctggtcac gaccgtgcat cgggttagca cccggtccga
     1141 acggcgcgcc agcacgacga ccgtctgggg tgttaccagt tttcttacca tacacaacgt
     1201 tagaagtgat ggtcagaaca gactgagtcg ggatagcgtc acggtaggtg tgcagtttct
     1261 gaattttctt catgaaacgt tctaccaggt caacagccag gtcatctaca cgcggatcat
     1321 tgttaccaaa ctgcgggtat tcgccttcga tttcgaagtc gatagccaga ccgtcttcgt
     1381 cacgaatcgg tttaactttc gcatatttga ttgcagacag ggagtcagca gcaacggaca
     1441 gaccagcgat accacacgcc atggtgcgga taacgtcacg gtcgtgcagc gccatcagag
     1501 aggcttcgta gctgtacttg tcgtgcatgt agtggatgat gttcagtgca gtgatgtact
     1561 gtttagccag ccagtccatg aagtgatcca tgcgctccat cacttcatcg tagctcagga
     1621 gatcgccttt gatcggttca gacttcggac caacctgcat tttcagtttt tcgtcaacgc
     1681 cgccgttgat tgcgtacagc atggttttcg ccaggtttgc acgcgcaccg aagaactgca
     1741 tttgtttacc aacgatcatc gggcttacgc agcaagcgat agcgtagtca tcgttgttga
     1801 agtccggacg catcaggtca tcgttctcat actgcagaga agaggtgtcg atggacactt
     1861 tagcggcgaa tttcttgaag ttcagcggca gtttttcaga ccacagaatg gtcatgttcg
     1921 gttccggaga cggccccata gtgtacaggg tgttcaggaa acggaagctg tttttggtaa
     1981 ccagggtacg accatcgagg cccataccac cgatagattc ggttgcccag attgggtcgc
     2041 cagagaacag ttcatcgtat tccggagtac gcaggaagcg aaccatacgc agtttcatga
     2101 ccaggtggtc aaccatttcc tgcgcttctt gttcggtgat cttgccagct ttcaggtcac
     2161 gttcgatgta cacatccagg aaggtggagg tacgaccgaa ggacattgca gcaccgttct
     2221 gagacttaac agcagccagg tagccgaagt aagtccactg gatagcttcc tgagcgttgg
     2281 tagccggacc agagatgtcg tagccgtatt tcgcagccat ttctttcatc tgacccagag
     2341 cgcggtgctg ttcagcgatt tcttcgcgca gacggatagt ctgttccagg tttacgccgt
     2401 tttccagatc agcctgtaga gaggtgaact gagcgtattt gtctttcatc aggtagtcga
     2461 taccgtacag cgcaacgcga cggtagtcac cgatgatacg gccacggcca taagcatctg
     2521 gcagaccggt cagaacaccg gatttacggc aacgcaggat gtccggagtg taaacgtcga
     2581 acacgccctg gttgtgagtt ttacggtatt cagtgaagat ttttttgatc atcgggtcca
     2641 gttcgcggtt gtacgctttg caggaacctt cgatcatttt gataccaccg aacgggataa
     2701 gagcacgttt cagcggagct tcagtctgca gaccaacaac tttttccaac gctttattga
     2761 tgtagccagc gtcgtgagag gtgatggtgg aagcaacagc ggtgtcaaaa tcaactggcg
     2821 cgtgagtgcg gttttccagt ttaacacctt ccattacttt gtcccacagg gtggtggtcg
     2881 cttcagtagc gccagccagg aaggactcgt caccctcgta cggagtgtag tttttctgaa
     2941 tgaagtcacg gacgtttact tcattctgcc agtcaccttt ggtaaaacct tcccaggctg
     3001 tggctaactt ttcattaagc tcggacatgt aacacctacc ttcttaagtg gattttttat
     3061 ttactgcgta cttcgacaac cattaatggt gatcgttttc acgcaggtaa atgacccagt
     3121 atgtcaaccc aaccaacaaa ccaccgccga taatattacc gatcgtaacc gggatcaggt
     3181 tatcagtgat gaagttcatc acggtcaggt gagaaaaatt ttccggtgca gaaccgacag
     3241 cggtccagaa ttccggagat gcgaagtcgc ggattacaat acccatcggg atcataaaca
     3301 tgtttgcgat actgtgctca aaaccgctgg caacaaacat cgcgaccggc agcaccataa
     3361 tgaacgcttt gtccatcagg ctgcggccag aataactcat ccatactgcc agacatacca
     3421 tcaggtttgc caggatacca agacagacgg cctcaataaa agtatggtgc actttgtggt
     3481 cggcggtttg taggacgttt agtccccatt gaccatttgc ggtcatatac tcgccggaaa
     3541 gccacattaa aagtacaaac agcagtgcgc cgaccaggtt gccaaaatag acatttagcc
     3601 agtttttagc caactgacac caggtgatgc gcccactcgc cttagcaaca acaatcaaca
     3661 ctgtggaagt aaagagatcg gctccgcaga caacacaaag aatcagcccc agagagaagc
     3721 aaatgccgcc aaccagtttt gccatgccga agggcattgt gcctgtgcca gtggttgctg
     3781 tgatatagaa gacgaatgcg attgagatga aaacaccggc ggtaatcgcc agatagaaag
     3841 tcttaagcgg atgtttcgtt gctttataga cacccgcctc ttcggccact ttggccattg
     3901 cagcaggaag taaaagatca aaagggttgt cagctttcac actaactctc tctttattaa
     3961 gtcggcgacg agatactaac aaagcattat agatgagaaa ttgatataga tcatatctcg
     4021 cctggcttat aggcccgtaa ctcgcatggt ttttatgcaa atacggagta actatttgat
     4081 tatccaaata aaaataaatt ttaaaaatta acaaatgagt tgaatttttt ccgcatcctc
     4141 cgctaaaaca gttaattaaa agggagcatc aggcgaataa agtaacaata tcgatcgtat
     4201 ttattaaata caaattaccg atatttaacc ttataattac aattatttta ttaatgcaaa
     4261 tatatgtaaa gcggggcatt aaaaaaacgc cccgtaatat aactcagact aatcattaag
     4321 cctacattgc gtaggctatt tgattttatt ttgcccagaa tgctgctttg gcgcgctgca
     4381 gcttttcgta ggccttcaac aacgactgat gtgcagcaaa cgcgtgcaga tcgctatcta
     4441 ccggttgcag gccgtaaaac gccgcttcgc cgctcattgc cgcactggcg gcttctacgg
     4501 catctgcgcc gtacatgcga acaaacgcat tcagatattg cagcggctgg cgatcttctt
     4561 cctgtgccag taataacaac gtttgcaggc agcgataata gttggcgcgt tccggactaa
     4621 atactgatga gttaaactcc atcgtccatt cggtccagac cagagcctgt tccagatcgc
     4681 caccagccag cgccagcatg gcttttaatt caccgatacg cagggtgtac caaccgttat
     4741 ccgacccggt cgccagaccc aacagctcac gcacgcgggt aaagtcatca aaaccttctt
     4801 catccagttg ctcgatgagg ttcaggtaat cttctttttc ccactcgctg cctggtagcg
     4861 aaagaatcgt ttcacgtaaa tggctgccca tactgttatt cgcgagccac agatcttcag
     4921 ccggataaat atcggacatg ccaggcacga taatacggca agcataaacg cccagatgct
     4981 cgtaatcggc aatataaact tctttatctt ctttgttgaa gatagccatc agcgtagcaa
     5041 actcttcttc cgtggtgccg gagaaattcc agtccacaaa cggataatcg gcatcctgct
     5101 tgaacaggtc ccaggagatt aaaccgctgg aatcgataaa gtgcgtttcg aggttggtat
     5161 gttcagcgac ttcttcatca tcgaaggttg gcggagtaaa cacatccaaa tctttcaggc
     5221 cacgaccttg cagcagctcg gtcacggtac gttccagtgc tacgccaaaa tcaggatgcg
     5281 caccgaaaga ggcaaagcag gtgccgtttg caggattaaa cagtaccacg caaatcaccg
     5341 gatactggcc gccaagggaa ccatcatatg cgaagatcgg gaaaccttct gtttccagtg
     5401 tttcgatcgc ttcaaccacc gctgggtaac gcgccagcac gtctgccggg atttccggca
     5461 agctgatgct ttcagcaata atgcggtttt tcacgtagcg ttcgaaaact tcggacaacc
     5521 cctgaacgcg tgcttcgttg cgggtattgc cagcggacat accgttagaa acgtacaggt
     5581 taccaatgat attcatcgga atataaacgg tctgattgtc ggactggcgc gtaaacggca
     5641 gaccgcaaat accacgatct tcgttaccgg attgtaggtc aatcagcata ctgccggtca
     5701 attcattctc cggatcgtaa aatgcgcgca gacggtcatc gagcagccct tctggcacat
     5761 cgtcattttc gctcagtggg aaccattttt cgttgggata atgcacaaat ggaccgttgg
     5821 cgatggtttc gcccagccag aaatcagcaa aaaagtagtt ggttgagaga cgctcgaaat
     5881 attcaccgag tgcagaagcc agcgccgctt tcttggttgc gcctttaccg ttggtaaaac
     5941 acagtgcgca ctctttgtcg cgaatatgta cagaccagac gttaggcacg ggattcagcc
     6001 aggaggcctc ttcaatctga aagccgaggt ctgaaagttt ttgctggaag cgagcgatgg
     6061 aatcttccag agcggcatct ttgccgggga taaatgtttg cgtcatgaaa atcactttag
     6121 tcgtacggaa agcgcgcaat aatacgggtt ttatctcaaa ggcgctatca ccgccgccat
     6181 gccgatgaac tgttgactat gcttttagcg gataaaccac gttaagagca taaaaatgaa
     6241 ggcgttcgat ctccaccgta tggcatttga taaagtgcct tttgatttcc ttggcgaagt
     6301 tgcactacgt agtctttata cctttgtact ggttttttgt tcctcaaaat gaccggaaga
     6361 cgcggtgtgc gacagatgtc gctgtttgaa gtgttaatca ttctcacgct gggttcagcg
     6421 gcgggagatg tggcgtttta tgatgatgtg ccgatggtgc cggtacttat cgtctttatc
     6481 actctggcgt tgttataccg cctggtaatg tgatggcgag ctggcctggt cgaaactcaa
     6541 taactccaac atgacggaat ttgagttctt tatggagcta cgattgcgtg gcgtggagca
     6601 gctggggcag gtacgtctgg cgattctcga aaccaacggg caaatcagtg tctatttctt
     6661 tgaagatgac aaggtgaaac cgggtttact tattttaccc agtgattgta cgcagcgtta
     6721 caaagtggtg ccggagtcgg cggactatgc ctgcattcgt tgtagtgaaa tcattcatat
     6781 gaacgcgggg gaaaaacaat tatgtccgcg ctgtgcaaat ccagaatgga cgaaggcaag
     6841 tcgggcaaaa cgggtgacct gacagtaaaa acatcggctt tttgctaata atccgagaga
     6901 ttcttttgtg tgatgcaagc cacatttttg ccctcaacgg ttttactcat tgcgatgtgt
     6961 gtcactgaat gataaaaccg atagccacag gaataatgta ttacctgtgg tcgcaatcga
     7021 ttgaccgcgg gttaatagca acgcaacgtg gtgaggggaa atggctcaaa tcttcaattt
     7081 tagttctggt ccggcaatgc taccggcaga ggtgcttaaa caggctcaac aggaactgcg
     7141 cgactggaac ggtcttggta cgtcggtgat ggaagtgagt caccgtggca aagagttcat
     7201 tcaggttgca gaggaagccg agaaggattt tcgcgatctt cttaatgtcc cctccaacta
     7261 caaggtatta ttctgccatg gcggtggtcg cggtcagttt gctgcggtac cgctgaatat
     7321 tctcggtgat aaaaccaccg cagattatgt tgatgccggt tactgggcgg caagtgccat
     7381 taaagaagcg aaaaaatact gcacgcctaa tgtctttgac gccaaagtga ctgttgatgg
     7441 tctgcgcgcg gttaagccaa tgcgtgaatg gcaactctct gataatgcgg cttatatgca
     7501 ttactgcccg aatgaaacca tcgacggtat cgccatcgac gagacgccag acttcggcaa
     7561 agatgtggtg gtcgccgccg acttctcttc aaccattctt tcccgtccga ttgacgtcag
     7621 ccgttatggt gtaatttacg ctggcgcgca gaaaaatatc ggcccggctg gcctgacaat
     7681 cgtcatcgtt cgtgaagatt tgctgggcaa agcgaatatc gcgtgtccgt cgattctgga
     7741 ttattccatc ctcaacgata acggctccat gtttaacacg ccgccgacat ttgcctggta
     7801 tctatctggt ctggtcttta aatggctgaa agcgaacggc ggtgtagctg aaatggataa
     7861 aatcaatcag caaaaagcag aactgctata tggggtgatt gataacagcg atttctaccg
     7921 caatgacgtg gcgaaagcta accgttcgcg gatgaacgtg ccgttccagt tggcggacag
     7981 tgcgcttgac aaattgttcc ttgaagagtc ttttgctgca ggccttcatg cactgaaagg
     8041 acaccgtgtg gtcggcggaa tgcgcgcgtc tatttataat gccatgccgc tggaaggcgt
     8101 taaagcgctg acagacttca tggttgagtt cgaacgccgt cacggttaat gccgaaattt
     8161 tgcttaatcc ccacagccag cctgtggggt ttttatttct gttgtagaga gttgagttca
     8221 tggaatccct gacgttacaa cccatcgctc gtgtcgatgg cactattaat ctgcccggtt
     8281 ccaagagcgt ttctaaccgc gctttattgc ttgcggcatt agcacacggc aaaacagtat
     8341 taaccaatct gctggatagc gacgacgtgc gccatatgct gaatgcatta acagcgttag
     8401 ggttaagcta tacgctttca gccgatcgta cgcgttgcga aattatcggt aacggcggtc
     8461 cattacacgc agaaggtgcc ctggagttgt tcctcggtaa cgccggaacg gcaatgcgtc
     8521 cgctggcggc agctctttgt ctggatagca atgatattgt gctgaccggt gagccgcgta
     8581 tgaaagagcg cccgattggt catctggtgg atgcgctgcg cctgggcggg gcgaagatca
     8641 cttacctgga acaagaaaat tatccgccgt tgcgtttaca gggcggcttt actggcggca
     8701 acgttgacgt tgatggctcc gtttccagcc aattcctcac cgcactgtta atgactgcgc
     8761 ctcttgcgcc ggaagatacg gtgattcgta ttaaaggcga tctggtttct aaaccttata
     8821 tcgacatcac actcaatctg atgaagacgt ttggtgttga aattgaaaat cagcactatc
     8881 aacaatttgt cgtaaaaggc gggcagtctt atcagtctcc gggtacttat ttggtcgaag
     8941 gcgatgcatc ttcggcttct tacttcctgg cagcagcagc aatcaaaggc ggcactgtaa
     9001 aagtgaccgg tattggacgt aacagtatgc agggtgatat tcgctttgct gatgtgctgg
     9061 aaaaaatggg cgcgaccatt tgctggggcg atgattatat ttcctgcacg cgtggtgaac
     9121 tgaac
//
LOCUS       DAJTPG010000135         9060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTPG010000135 DAJTPG010000000
VERSION     DAJTPG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..913
                     /locus_tag="OO230_003543"
     CDS             <1..913
                     /locus_tag="OO230_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5693164.1"
                     /translation="EAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPPALV
                     LMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQLQF
                     SEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLA
                     AAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFIS
                     MALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSF
                     NLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1165..2082
                     /gene="oxyR"
                     /locus_tag="OO230_003544"
     CDS             1165..2082
                     /gene="oxyR"
                     /locus_tag="OO230_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5693165.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2065..3465)
                     /gene="sthA"
                     /locus_tag="OO230_003545"
     CDS             complement(2065..3465)
                     /gene="sthA"
                     /locus_tag="OO230_003545"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5693166.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3786..4436
                     /gene="fabR"
                     /locus_tag="OO230_003546"
     CDS             3786..4436
                     /gene="fabR"
                     /locus_tag="OO230_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5693167.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4436..4795
                     /locus_tag="OO230_003547"
     CDS             4436..4795
                     /locus_tag="OO230_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5693168.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4835..5935)
                     /gene="trmA"
                     /locus_tag="OO230_003548"
     CDS             complement(4835..5935)
                     /gene="trmA"
                     /locus_tag="OO230_003548"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5693169.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6049..6239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6304..8148
                     /gene="btuB"
                     /locus_tag="OO230_003549"
     CDS             6304..8148
                     /gene="btuB"
                     /locus_tag="OO230_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5693170.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8093..8950
                     /gene="murI"
                     /locus_tag="OO230_003550"
     CDS             8093..8950
                     /gene="murI"
                     /locus_tag="OO230_003550"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5693171.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2065 a         2196 c         2443 g         2356 t
ORIGIN      
        1 cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg cacaaaaaga
       61 acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg ctatcgcgcc
      121 tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg ttatcatcgg
      181 cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc gggcgcagtt
      241 tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc gtccacagct
      301 gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga tgattttagg
      361 tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg gttatttgca
      421 agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc ctttcctcgc
      481 ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga aaaaaggcta
      541 cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt cggcattagg
      601 cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta tctcaatggc
      661 gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg tgatggtgtc
      721 agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg tttttgcttc
      781 ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta atctggcgct
      841 ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta ttgtcaaaga
      901 tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga ataaaaaaaa
      961 gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt tgagaaactc
     1021 tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct gtgagcaatt
     1081 atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat cgccatgaac
     1141 tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct ggtggcattg
     1201 gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca gccgacgctt
     1261 agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga gcggaccagc
     1321 cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg taccgtgctg
     1381 cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat gtccggaccg
     1441 ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat tatcccgatg
     1501 ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac ccaccagtta
     1561 ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt gaaagagagc
     1621 gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat ctatgaagat
     1681 cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga aaaactgctg
     1741 atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt tgaagccggg
     1801 gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa catggtggcg
     1861 gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg caaacgcgat
     1921 ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg cctggtttat
     1981 cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat ccgcgcaaga
     2041 atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta acgcagctac
     2101 ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt actcaatagt
     2161 gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt cggcagcgcg
     2221 ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca aaattttcag
     2281 cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact gggcgcggcc
     2341 cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca cagagctgat
     2401 ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg tggcttcgcc
     2461 ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg ccaggctcgg
     2521 ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat acatgctgtt
     2581 gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg ccagcgaatc
     2641 ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt ttttacccga
     2701 cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact cttcgttgtg
     2761 acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca tctcttgatc
     2821 gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat ccataccgcg
     2881 gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta catggcgcgg
     2941 ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg tgaaatcaac
     3001 atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag cggttagtgt
     3061 ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa agcgagcgtt
     3121 cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc gcgtttgttg
     3181 attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga gcagtcggga
     3241 atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg cgtgacggag
     3301 agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt gataacgctc
     3361 gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt cgccgccggg
     3421 gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag ggcttacctg
     3481 ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc aattgatccc
     3541 cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt acttttgccc
     3601 tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg tttccgctgt
     3661 acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc acaatactgg
     3721 agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc aaggattcag
     3781 acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg ctggtggaag
     3841 ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg cgtgaagtgg
     3901 cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac gtagacgaac
     3961 tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg cgccaggcgc
     4021 gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca tttatggagt
     4081 tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc ggcacctccg
     4141 ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa cttgcggact
     4201 atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc gaagcaatgg
     4261 tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa caacgtcggc
     4321 aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct tattactggt
     4381 atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac gagtaatgaa
     4441 acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt tatcgattaa
     4501 tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc cgattatttc
     4561 cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca tgtcggtagg
     4621 cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca gcacggtagt
     4681 tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct ccgtcattat
     4741 ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg agtaatcggt
     4801 atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt aatacgccgc
     4861 actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg accttgtgcg
     4921 tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag atatacagaa
     4981 tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg cgcggtgggt
     5041 cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg cggttaaact
     5101 cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata atttgtacgt
     5161 tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt ttggcgattt
     5221 cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta ccgttgccgc
     5281 agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc cattccagca
     5341 tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga cgatagatca
     5401 tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc gcgattttgg
     5461 ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt gcatcgcgca
     5521 gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc agggaaacca
     5581 ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg cgcagaacgg
     5641 gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt tcactggcgg
     5701 cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg atgtgataca
     5761 ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga ctgaccggcg
     5821 agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc aaacgtacca
     5881 ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg gtcatgatgt
     5941 atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg tccagatctt
     6001 gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct gtagcatcca
     6061 cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga cgcgcagcgg
     6121 taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga agtcatcatc
     6181 tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt cgcatctggt
     6241 tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg tggatgcttt
     6301 acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt ttccgcttgg
     6361 gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga acagccgcgc
     6421 agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg ctggcagtcg
     6481 acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca aaacggcggt
     6541 tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt gctggtgtta
     6601 attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct tagccagttc
     6661 cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc tgtttatggt
     6721 tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc cggaacggaa
     6781 atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac gcagcaacaa
     6841 ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca tggttatgat
     6901 gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg ttttttaagt
     6961 aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg ctttgttcgc
     7021 ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg ttcaccgttg
     7081 ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta taacggcgaa
     7141 ctgattaaat cacaactcat taccagctat agccatagca aagattacaa ctacgatccc
     7201 cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata caccgtccag
     7261 tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga ctggcagaaa
     7321 cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg taataccggc
     7381 atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc agcacgcagc
     7441 gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg ttgggaattc
     7501 atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact aaatctgggg
     7561 caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa acagtgggaa
     7621 ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata tcgtaacgat
     7681 gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga agggaaagcg
     7741 cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac gcatactgtg
     7801 agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt acgccgtgct
     7861 aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg gggtattact
     7921 tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta tcaaaccgtt
     7981 aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac ctctcacctg
     8041 acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt ctatggctac
     8101 caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac cacgtcccac
     8161 cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga tccggcatct
     8221 cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt atggcgaaaa
     8281 aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc aagaacgtta
     8341 tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac ttcctgcatt
     8401 acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac ctgctgcacg
     8461 tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta aacgttctta
     8521 tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc tgggctcggc
     8581 agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc tggatgcact
     8641 aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg ttgtattggg
     8701 ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag agggaacccg
     8761 gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag aacatgaagc
     8821 cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga cgccagaagc
     8881 tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa aactggcagt
     8941 tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt tcctcttgtc
     9001 aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc aaacacgccg
//
LOCUS       DAJTPG010000136         9019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTPG010000136 DAJTPG010000000
VERSION     DAJTPG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO230_003551"
     CDS             complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO230_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5693172.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO230_003552"
     CDS             complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO230_003552"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5693173.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO230_003553"
     CDS             complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO230_003553"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5693174.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO230_003554"
     CDS             complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO230_003554"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5693175.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4456..5580
                     /gene="dprA"
                     /locus_tag="OO230_003555"
     CDS             4456..5580
                     /gene="dprA"
                     /locus_tag="OO230_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5693176.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5552..6025
                     /locus_tag="OO230_003556"
     CDS             5552..6025
                     /locus_tag="OO230_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5693177.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6054..6596
                     /locus_tag="OO230_003557"
     CDS             6054..6596
                     /locus_tag="OO230_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5693178.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6601..7173
                     /gene="tsaC"
                     /locus_tag="OO230_003558"
     CDS             6601..7173
                     /gene="tsaC"
                     /locus_tag="OO230_003558"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5693179.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7178..7996
                     /gene="aroE"
                     /locus_tag="OO230_003559"
     CDS             7178..7996
                     /gene="aroE"
                     /locus_tag="OO230_003559"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5693180.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7993..8250
                     /locus_tag="OO230_003560"
     CDS             7993..8250
                     /locus_tag="OO230_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5693181.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8226..8780)
                     /locus_tag="OO230_003561"
     CDS             complement(8226..8780)
                     /locus_tag="OO230_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5693182.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2253 a         2190 c         2252 g         2324 t
ORIGIN      
        1 ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa agggaaataa
       61 taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg gaagagtctt
      121 tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca
      181 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
      241 gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca
      301 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga ggaaacacca
      361 acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa
      421 attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
      481 accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcctcg
      541 tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
      601 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg
      661 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
      721 gccagccctg caccgatatt accgccacca accagcatga tccgtttata cggtttttcc
      781 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
      841 tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc gtggcggaaa
      901 attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta
      961 ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt accctcagcg
     1021 aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt atcgataacc
     1081 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagcttatcg
     1141 gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt gttgaaaagt
     1201 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc
     1261 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
     1321 acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca
     1381 acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
     1441 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
     1501 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt acccggttgc
     1561 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
     1621 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
     1681 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttccgggata
     1741 tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc cgttgctgaa
     1801 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtaa
     1861 cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt gtcgtaaatg
     1921 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
     1981 aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg ttcaccgttt
     2041 tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt aacccatccg
     2101 tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac agcatcaggg
     2161 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
     2221 cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt attggcttcg
     2281 gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
     2341 tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg ccgacgctgg
     2401 aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat tgcgatagcg
     2461 ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag cagttgatac
     2521 aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg acgggccatt
     2581 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
     2641 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
     2701 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
     2761 cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag agtggactat
     2821 cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag gtcttgcgcg
     2881 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcaccagtc
     2941 gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc gttggttgcc
     3001 gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
     3061 attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga aagtgaccag
     3121 tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt ttcgtcctga
     3181 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
     3241 ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt aatcgggcag
     3301 gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat aatggttaca
     3361 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
     3421 ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag cacagcttta
     3481 ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc ggcgaccagt
     3541 tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc ttcttcctca
     3601 gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
     3661 tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag atgacgcgct
     3721 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
     3781 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt cgttgttgtt
     3841 tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc tcatgctgaa
     3901 tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg cggtcaaggg
     3961 cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg atcgacaggc
     4021 aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
     4081 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcaacct
     4141 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
     4201 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
     4261 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
     4321 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc acttgattga
     4381 cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag gcttgcttat
     4441 ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag catcagcagc
     4501 ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc gcatattgat
     4561 gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt tctttcattt
     4621 ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca tcatttaatc
     4681 cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta ccccggcgca
     4741 ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt agtggggagt
     4801 cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac tctggcgacg
     4861 tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt gcataaagcg
     4921 gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa taccattcat
     4981 ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc tctcgtctcg
     5041 gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa tcgcattatc
     5101 agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg
     5161 acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg tccaataggg
     5221 aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct tgtgacggaa
     5281 ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga cgcccctgaa
     5341 aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga gctcctggct
     5401 aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg
     5461 ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc agctgtaccc
     5521 ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt atttgtttga
     5581 aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg aacaggatct
     5641 taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc tggaaaaact
     5701 tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc ctctctccat
     5761 gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg ggtttctgct
     5821 tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga tagagcgagt
     5881 gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga tcctgatggt
     5941 gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat tactctttga
     6001 agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt gttatggcaa
     6061 aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg gctgaactgg
     6121 ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg gcgtgtgact
     6181 acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg gaggggcagg
     6241 tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt atgtttattg
     6301 gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg gacgaaacag
     6361 caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc cgttatggca
     6421 aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa
     6481 tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa accgcgcagg
     6541 gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg gaataataac
     6601 gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct caatgaaaaa
     6661 cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc tgatagcgaa
     6721 acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg gctgatttta
     6781 atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt gactgacgtg
     6841 cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt tcccgcgcct
     6901 gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg agtcaccgac
     6961 catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc taccagtgcc
     7021 aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg
     7081 gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga aatccgcgat
     7141 gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg ctgtttttgg
     7201 taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc agcaactgaa
     7261 tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca acacactgaa
     7321 cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc
     7381 ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag ttaataccct
     7441 caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag gcttgttaag
     7501 cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta tcggcgctgg
     7561 tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg tgacaatcac
     7621 taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca ctggcagtat
     7681 tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta ttaatgcaac
     7741 atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc atccaggcat
     7801 ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat ggtgtgagca
     7861 gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg cggctcatgc
     7921 ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc aattgcagga
     7981 ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg ggacgagaat
     8041 aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg cgcgatctct
     8101 ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt agcgagtttt
     8161 cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga acaaagtgaa
     8221 gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca tttcacgtaa
     8281 ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg gatctgtttg
     8341 acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg gaccagacta
     8401 cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat tgagcccatc
     8461 ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg accaacagtg
     8521 acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt atgagtgaca
     8581 tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata atgtacatct
     8641 ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac gtcaccaatc
     8701 acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag atcgcggtat
     8761 gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat gactttgggg
     8821 gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgatt
     8881 gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat acttgtgcaa
     8941 aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacagga
     9001 tggtcggaac aacgaagag
//
LOCUS       DAJTPG010000137         9001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTPG010000137 DAJTPG010000000
VERSION     DAJTPG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="OO230_003562"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="OO230_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5693183.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="OO230_003563"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="OO230_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5693184.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO230_003564"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO230_003564"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5693185.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO230_003565"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO230_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5693186.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="OO230_003566"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="OO230_003566"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5693187.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO230_003567"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO230_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5693188.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..>9001
                     /gene="entF"
                     /locus_tag="OO230_003568"
     CDS             5545..>9001
                     /gene="entF"
                     /locus_tag="OO230_003568"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5693189.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITLQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLL"
BASE COUNT         1946 a         2379 c         2479 g         2197 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcttggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat taccctccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 g
//
LOCUS       DAJTPG010000138         8790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTPG010000138 DAJTPG010000000
VERSION     DAJTPG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OO230_003569"
     CDS             complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="OO230_003569"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5693190.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OO230_003570"
     CDS             complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="OO230_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5693191.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="OO230_003571"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="OO230_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5693192.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO230_003572"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO230_003572"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5693193.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO230_003573"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO230_003573"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5693194.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="OO230_003574"
     CDS             complement(7826..8137)
                     /locus_tag="OO230_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5693195.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO230_003575"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO230_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5693196.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8649..>8790)
                     /locus_tag="OO230_003576"
     CDS             complement(8649..>8790)
                     /locus_tag="OO230_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5693197.1"
                     /translation="WNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAH
                     QY"
BASE COUNT         2200 a         2278 c         2081 g         2231 t
ORIGIN      
        1 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgtggcgt gaacgcctta
       61 tccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
      121 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
      181 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatcttt caggggaatt
      241 ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg
      301 atcgcgattc agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc
      361 gatttccagc accttactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt
      421 aaatgacggg ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat
      481 ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
      541 gaacagtttc cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac
      601 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc
      661 cagccacgcc atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt
      721 gaacgcttcg ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
      781 ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag
      841 cagcacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta
      901 ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag
      961 caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg
     1021 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa
     1081 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     1141 cgtcaggaac accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt
     1201 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     1261 caattcttca taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg
     1321 catcaacgcc gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc
     1381 cggcagcgga gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg
     1441 tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     1501 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     1561 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca
     1621 taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     1681 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg
     1741 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     1801 ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg
     1861 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg
     1921 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     1981 ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag
     2041 gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag
     2101 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc
     2161 ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt
     2221 catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     2281 gatggtacca acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     2341 cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     2401 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg
     2461 atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg
     2521 ttccagacgc gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg
     2581 ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc
     2641 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
     2701 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
     2761 tgattttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
     2821 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
     2881 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
     2941 cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
     3001 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
     3061 tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt
     3121 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
     3181 ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat
     3241 cgagatcttc aaagcgggag aaatcacgac ctcgttgcaa cagaccaaag cctcgcgggt
     3301 tttccatgga gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc
     3361 attcgccatt accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt
     3421 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc
     3481 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
     3541 gccctggcat aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat
     3601 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
     3661 gaaattcttc acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat
     3721 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
     3781 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
     3841 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
     3901 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
     3961 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
     4021 gattaaactg gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt
     4081 tgcttttagg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
     4141 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
     4201 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctaacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt
     4921 accgcaattt attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt
     5281 tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa catcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgcaccaaa ataggcatca ccaatatttc gtcctcccac tagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg
     6841 atatatccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cgtggatgac tgtcaagcaa gcgcaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgccccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta actataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttccccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc
     7921 tcattaacgc tttgctggag taggttttgc ccggaataga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg aaggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 tccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca
     8521 tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa
     8641 atgatgtatt aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg
     8701 acggaggagt tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta
     8761 acagtcattt cagaattttt gccgttccac
//
LOCUS       DAJTPG010000139         8771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTPG010000139 DAJTPG010000000
VERSION     DAJTPG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO230_003577"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO230_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5693198.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="OO230_003578"
     CDS             complement(984..1814)
                     /locus_tag="OO230_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5693199.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2024..3037
                     /gene="intI1"
                     /locus_tag="OO230_003579"
     CDS             2024..3037
                     /gene="intI1"
                     /locus_tag="OO230_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5693200.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3240..3590
                     /locus_tag="OO230_003580"
     CDS             3240..3590
                     /locus_tag="OO230_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5693201.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3716..4276
                     /locus_tag="OO230_003581"
     CDS             3716..4276
                     /locus_tag="OO230_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5693202.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4279..7245
                     /locus_tag="OO230_003582"
     CDS             4279..7245
                     /locus_tag="OO230_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5693203.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7312..7689
                     /locus_tag="OO230_003583"
     CDS             7312..7689
                     /locus_tag="OO230_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5693204.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7890..8549)
                     /locus_tag="OO230_003584"
     CDS             complement(7890..8549)
                     /locus_tag="OO230_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5693205.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2480 c         2348 g         1993 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc ccaactttgt tttagggcga ctgccctgct gcgtaacatc
     1921 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1981 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     2101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg
     2161 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     2221 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     2281 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     2341 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     2401 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg
     2461 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat
     2521 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc
     2581 cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg
     2641 ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc
     2701 ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt
     2761 cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga
     2821 acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc
     2881 cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt
     2941 ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc
     3001 gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg
     3061 gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt
     3121 cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct
     3181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa
     3241 tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg
     3301 gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg
     3361 gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct
     3421 gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gccggaaggg gccgagtacg
     3481 tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga
     3541 ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct
     3601 aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat
     3661 tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac
     3721 tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact
     3781 ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg
     3841 tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag
     3901 catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca
     3961 acgcggcgtg catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc
     4021 gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat
     4081 ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag
     4141 gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga
     4201 gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt
     4261 gagaacggat cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc
     4321 ctgctggcgt tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc
     4381 gacctctcga tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag
     4441 ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc
     4501 ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc
     4561 cagcgggagc agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg
     4621 cccttcacca tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa
     4681 accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc
     4741 attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac
     4801 cggcgcatct acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac
     4861 gatctgctca agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct
     4921 ccggccaagc caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag
     4981 gcactcgatc tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt
     5041 gcccgcgagg gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc
     5101 tacgccactc tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc
     5161 gacctgcacg accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag
     5221 cagttccagg cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt
     5281 caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc
     5341 atgtcctggg attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat
     5401 gacttcgatt tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg
     5461 gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc
     5521 attgaggtgc tgcgcggcat gaacaccgac aacgcccgca agctgccagc cgatgcaccg
     5581 accggcttca tcaagccgcg ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg
     5641 cgctactacg aactgtgcgc gctgtccgag ttgaagaact ccctgcgctc gggcgacatc
     5701 tgggtgcagg gttcacgcca gttcaaggac ttcgaggact acctggtacc gcccgagaag
     5761 ttcaccagcc tcaagcagtc cagcgaattg ccgctggccg tggccaccga ctgcgaacaa
     5821 tatctgcatg agcggctgac gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg
     5881 gcagccaacg acctgccgga tgccatcatc accgagtcgg gcttgaagat cacgccgctg
     5941 gatgcggcgg tgcccgacac cgcgcaggcg ctgatagacc agacagccat ggtcctgccg
     6001 cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt ggacgggctt cacccggcac
     6061 ttcacgcact tgaaatcggg cgatctggcc aaggacaaga acctgttgtt gaccacgatc
     6121 ctggccgacg cgatcaacct gggcctgacc aagatggccg agtcctgccc cggcacgacc
     6181 tacgcgaagc tcgcttggct gcaagcctgg catacccgcg acgaaacgta ctcgacagcg
     6241 ttggctgaac tggtcaacgc tcagtttcgg catccctttg ccgggcactg gggcgatggc
     6301 accacatcat catcggacgg acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg
     6361 cacatcaacc caaaatatgg cagcagccca ggacggactt tctacaccca catctccgac
     6421 caatacgcgc cattccacac caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg
     6481 ctcgacggcc tgctgtacca cgaatccgac ctgcggatcg aggagcacta caccgacacg
     6541 gcgggcttca ccgatcacgt cttcgccctg atgcacctct tgggcttccg cttcgcgccg
     6601 cgcatccgcg acctgggcga caccaagctc tacatcccga agggcgatgc cgcctatgac
     6661 gcgctcaagc cgatgatcgg cggcacgctc aacatcaagc acgtccgcgc ccattgggac
     6721 gaaatcctgc ggctggccac ctcgatcaag cagggcacgg tgacggcctc gctgatgctc
     6781 aggaaactcg gcagctaccc gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc
     6841 cgcatcgagc gcacgctgtt catcctcgac tggctgcaaa gcgtcgagct acgccgccgc
     6901 gtgcatgccg ggctgaacaa gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc
     6961 aaccgccttg gtgaaatccg tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc
     7021 ctcaacctgg tgacggcggc catcgtgctg tggaacacgg tctacctgga gcgtgcggcg
     7081 catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc tattgcagta cctgtcgcca
     7141 ctcggctggg agcacatcaa cctgaccggt gattacctat ggcgcagcag cgccaagatc
     7201 ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg cttagcgtgc tttattttcc
     7261 gttttctgag acgaccccta ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat
     7321 tatctctgta aaaagttatt caaccggctt tccggtaccc tggtgatcag agcgcgctgc
     7381 ggcaataaca ttactggcct ggcatgttgc aatattcttt atccctcgcc ccgatacagc
     7441 ggtcagctgc atattaaaga gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt
     7501 aaagcgataa tgcgctttat agcccggctt gcgcttgaac aggaatgcct tagccttagc
     7561 tggaacgctg aaaaatccaa ccccggcgct aaccgttttt atcaggctct gggaggcaga
     7621 ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca
     7681 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     7741 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     7801 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
     7861 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     7921 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     7981 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     8041 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     8101 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     8161 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     8221 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     8281 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     8341 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     8401 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     8461 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     8521 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     8581 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     8641 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     8701 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     8761 caaagtgcgt c
//
LOCUS       DAJTPG010000140         8762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTPG010000140 DAJTPG010000000
VERSION     DAJTPG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..591)
                     /locus_tag="OO230_003585"
     CDS             complement(<1..591)
                     /locus_tag="OO230_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5693206.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(658..1383)
                     /gene="fimC"
                     /locus_tag="OO230_003586"
     CDS             complement(658..1383)
                     /gene="fimC"
                     /locus_tag="OO230_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5693207.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1420..1959)
                     /locus_tag="OO230_003587"
     CDS             complement(1420..1959)
                     /locus_tag="OO230_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5693208.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="OO230_003588"
     CDS             complement(2024..2572)
                     /gene="fimA"
                     /locus_tag="OO230_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5693209.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3053..3649)
                     /gene="fimE"
                     /locus_tag="OO230_003589"
     CDS             complement(3053..3649)
                     /gene="fimE"
                     /locus_tag="OO230_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5693210.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4127..4729)
                     /gene="fimB"
                     /locus_tag="OO230_003590"
                     /pseudo
     CDS             complement(4127..4729)
                     /gene="fimB"
                     /locus_tag="OO230_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6183..6899
                     /gene="nanC"
                     /locus_tag="OO230_003591"
     CDS             6183..6899
                     /gene="nanC"
                     /locus_tag="OO230_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5693211.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6919..8025
                     /gene="nanM"
                     /locus_tag="OO230_003592"
     CDS             6919..8025
                     /gene="nanM"
                     /locus_tag="OO230_003592"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5693212.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8090..>8762
                     /locus_tag="OO230_003593"
     CDS             8090..>8762
                     /locus_tag="OO230_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5693213.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFC"
BASE COUNT         2571 a         1913 c         1819 g         2459 t
ORIGIN      
        1 ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca
       61 taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag
      121 gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca ttgtggttaa
      181 tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg tattcagccc
      241 catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt
      301 attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata
      361 cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg
      421 atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag gtgcctgtgc
      481 ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat gcttacgaat
      541 atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc
      601 ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta
      661 ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt
      721 aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa
      781 tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt aatagggtgt
      841 cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg
      901 atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc
      961 gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt
     1021 catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg
     1081 taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc
     1141 atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac tattttcatc
     1201 attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc
     1261 acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat
     1321 acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact
     1381 cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg ttaaagagaa
     1441 ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc
     1501 gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt
     1561 aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg aggctatccc
     1621 tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac cgtgaaacgc
     1681 cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac
     1741 cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc ccatattgac
     1801 cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa
     1861 ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag caaacattgg
     1921 cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta tcgcacaagg
     1981 gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga tactgaacct
     2041 tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca taataacgcg
     2101 cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta aatgtcgcac
     2161 cgtccagcgt cagagcattg cctgttctgt ctaggatctg cacgccaacg tttgttgcac
     2221 tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca attgccgtac
     2281 ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc agctgaatgt
     2341 taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta cgaacctgtc
     2401 ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt
     2461 cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga gccgctgcgg
     2521 aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt
     2581 cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc
     2641 aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca
     2701 ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg ttggcttaat
     2761 attctattat tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa
     2821 gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg gaggtgatgt
     2881 gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt tttgtcgttt
     2941 tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg acagtttggc
     3001 cccaattgtc ttgtgtttat ttgtttttta actttattat caataagtta aatcaaacct
     3061 cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag
     3121 cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgatgcccg agataatcct
     3181 gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca
     3241 tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga ataatgcgat
     3301 aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg tcggtccggt
     3361 cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca cgctcatcaa
     3421 aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt
     3481 cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc atcccatgcc
     3541 gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct
     3601 gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac
     3661 tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc
     3721 ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa ttacattaaa
     3781 catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg atttgattca
     3841 atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa gaaaaaagtg
     3901 tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt atttattttg
     3961 aagaacataa atcattagat tttgtatggg tatggttaac ttaatgttta ttatgataat
     4021 taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt
     4081 aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcat
     4141 gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg
     4201 tataccagac agtatgacga atattgcgat gcccaagata atcctagata agtcgcgtat
     4261 ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc ggatgaatct
     4321 ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac tgttgccgag
     4381 aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc ggataagaag
     4441 tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc gggtgcgttg
     4501 ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga tcaatatccg
     4561 aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaaatca
     4621 gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt gactcgattt
     4681 cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc gttttccctt
     4741 ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt tttatcctga
     4801 ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca gttcttctga
     4861 gcgaacttaa atggagtagc gatagttact tataataact ttgccataca aacgccatgc
     4921 tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa taataaccct
     4981 catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta acaagtaaaa
     5041 tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc aaatatgaca
     5101 ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt aatttcttag
     5161 tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg ttcgtcagct
     5221 taggccatga gattgttaca ttccttttta tcctggagat atgggggaac gcaggcgacg
     5281 ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta agccttcctc
     5341 aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc atcctgaaaa
     5401 caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac aagccacaaa
     5461 ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta aacccttatt
     5521 ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct aaataaatta
     5581 tttataaaac gtatagttaa aagaaaatct ttagtttttg aagaccaccg caagtgttcg
     5641 tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt gacgcgaatt
     5701 gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac aggtataaag
     5761 atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac atcacttcag
     5821 ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga aaatgagatc
     5881 aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat ataacttacg
     5941 aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta atctggatct
     6001 ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac aaaaaatata
     6061 aaatacagtt atccgttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat
     6121 aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat
     6181 ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa
     6241 tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgcctatg
     6301 agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta
     6361 atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg
     6421 ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa
     6481 cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga
     6541 caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag
     6601 acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc
     6661 atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaatgatt
     6721 atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta caataccata
     6781 tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag ggtgtttaca
     6841 acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt
     6901 agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat gatggcttca
     6961 tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag tggtaccgga
     7021 gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc atggtacaag
     7081 ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc tggtggaccg
     7141 agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg cggcattggc
     7201 aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc
     7261 aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca tgtgactttt
     7321 gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt caatggctat
     7381 tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat caacgcccac
     7441 tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc
     7501 tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc tggtgcggcg
     7561 gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga
     7621 acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgat
     7681 cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa tgattctctt
     7741 atatttgctg gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac
     7801 tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg gcataacggg
     7861 aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg
     7921 aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca
     7981 gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt cagggccccg
     8041 gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata tgaacgcaat
     8101 aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta
     8161 tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa ttaaacaatt
     8221 agcgagattt gcgcatacgc atcccggagg cccgccatgt cactttaacg acattattcc
     8281 actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac
     8341 gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact
     8401 gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc
     8461 ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg
     8521 tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc gagccgcact
     8581 ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg aatttgactt
     8641 aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg
     8701 tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg
     8761 cg
//
LOCUS       DAJTPG010000141         8759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTPG010000141 DAJTPG010000000
VERSION     DAJTPG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            7..438
                     /locus_tag="OO230_003594"
     CDS             7..438
                     /locus_tag="OO230_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5693214.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO230_003595"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(585..2747)
                     /gene="uvrD"
                     /locus_tag="OO230_003595"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5693215.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO230_003596"
     CDS             complement(2831..3547)
                     /gene="yigB"
                     /locus_tag="OO230_003596"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5693216.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO230_003597"
     CDS             complement(3547..4443)
                     /gene="xerC"
                     /locus_tag="OO230_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5693217.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4440..5147)
                     /locus_tag="OO230_003598"
     CDS             complement(4440..5147)
                     /locus_tag="OO230_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5693218.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO230_003599"
     CDS             complement(5144..5968)
                     /gene="dapF"
                     /locus_tag="OO230_003599"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5693219.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6005..6208)
                     /locus_tag="OO230_003600"
     CDS             complement(6005..6208)
                     /locus_tag="OO230_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5693220.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6397..7875
                     /locus_tag="OO230_003601"
                     /pseudo
     CDS             6397..7875
                     /locus_tag="OO230_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7872..>8759
                     /locus_tag="OO230_003602"
     CDS             7872..>8759
                     /locus_tag="OO230_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5693221.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2031 a         2388 c         2251 g         2089 t
ORIGIN      
        1 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
       61 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt
      121 aaagatattc agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat
      181 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
      241 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
      301 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat
      361 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
      421 gtcgagcgga aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc
      481 ggatgcggcg taaacgcctt atccggccta catggcgttg caatttattg aatctgaatg
      541 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
      601 cgggcgtaag ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg
      661 ctatgttcac cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
      721 acacgctggc cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga
      781 tggctgaccg ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc
      841 aactcgccaa taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt
      901 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg
      961 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct
     1021 tccataccga cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc
     1081 agttgtaccg catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg
     1141 gataggaacg cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc
     1201 gttgccgtca ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc
     1261 tgctcgtaca tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     1321 ggcatatcgg cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg
     1381 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     1441 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     1501 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     1561 cgcagatacg agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     1621 atacggtacg gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta
     1681 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     1741 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     1801 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     1861 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     1921 atttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     1981 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     2041 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     2101 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     2161 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac
     2221 gcctgataca ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc
     2281 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     2341 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg
     2401 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     2461 cggtgcgcca gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt
     2521 tgtccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc
     2581 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     2641 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     2701 tcgcgttgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     2761 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     2821 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca
     2881 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct
     2941 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     3001 tctcgccgat cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt
     3061 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     3121 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     3181 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa
     3241 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     3301 cttgttcaat cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt
     3361 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     3421 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     3481 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     3541 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     3601 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     3661 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     3721 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     3781 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     3841 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     3901 aatcggcagg cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag
     3961 gtcgaggtgt ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat
     4021 cacttccagc attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag
     4081 ccgattcata tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga
     4141 aactcctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     4201 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     4261 tacagcaaaa ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc
     4321 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct
     4381 aagctgacgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     4441 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     4501 aactgcgttc cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     4561 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc
     4621 ggtagcacca ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     4681 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     4741 gacggcgcac ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg
     4801 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     4861 agactgcgct gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc
     4921 atcaacaacg ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc
     4981 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     5041 cgcgcattgc ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     5101 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     5161 catcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     5221 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct
     5281 gaatccctac cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     5341 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     5401 tggcgcgctc cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg
     5461 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     5521 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     5581 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     5641 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     5701 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca
     5761 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     5821 gctcaaccac cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa
     5881 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     5941 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     6001 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     6061 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     6121 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6181 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6241 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6301 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6361 agtttcgtga tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6421 ttgttgtctt gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa
     6481 cataatattt accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca
     6541 aatctttctc tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata
     6601 gacccgacaa cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac
     6661 tacctctatg agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc
     6721 aatatcctct acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc
     6781 gcctcgttca aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc
     6841 aagcgcaccg atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg
     6901 gaattccggc ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc
     6961 ttgtatatca atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag
     7021 tcctgggatc agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt
     7081 ggcccctggg ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt
     7141 gatgccgacc cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg
     7201 cgtgataaaa acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa
     7261 gatttaacaa agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa
     7321 ggacctgctt gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc
     7381 agtgatgctg tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt
     7441 aaccgtaagc tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc
     7501 aacgccggga aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc
     7561 ggtttacacg tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat
     7621 gaacgcgaag cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac
     7681 cgctacattt acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa
     7741 caagttcgct taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa
     7801 acgggatatc tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat
     7861 gaggcgacaa aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat
     7921 ttctgacgcc tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt
     7981 cttacggtaa aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca
     8041 gggccggttt gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg
     8101 aaataagcat agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc
     8161 gtggcaaaat cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg
     8221 ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa
     8281 gtaatagcgg cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta
     8341 ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg
     8401 atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca
     8461 gaaattcata ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg
     8521 ttcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc
     8581 tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag
     8641 cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta
     8701 aacagctcac gcccgaccgt cagtggagca agtggataga cctaccaggt attgaacct
//
LOCUS       DAJTPG010000142         8758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTPG010000142 DAJTPG010000000
VERSION     DAJTPG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO230_003603"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO230_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5693222.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO230_003604"
     CDS             1828..3147
                     /locus_tag="OO230_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5693223.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO230_003605"
     CDS             3445..3486
                     /locus_tag="OO230_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693224.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO230_003606"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO230_003606"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5693225.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO230_003607"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO230_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO230_003608"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO230_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO230_003609"
     CDS             5815..6711
                     /locus_tag="OO230_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5693226.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO230_003610"
     CDS             6708..7682
                     /locus_tag="OO230_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5693227.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO230_003611"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO230_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..>8758)
                     /locus_tag="OO230_003612"
     CDS             complement(8657..>8758)
                     /locus_tag="OO230_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HCR5693228.1"
                     /translation="ASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2133 a         2134 c         2216 g         2275 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggc
//
LOCUS       DAJTPG010000143         8678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTPG010000143 DAJTPG010000000
VERSION     DAJTPG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO230_003613"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO230_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5693229.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3216)
                     /gene="hemX"
                     /locus_tag="OO230_003614"
     CDS             complement(1969..3216)
                     /gene="hemX"
                     /locus_tag="OO230_003614"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5693230.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQGDTPAAAPQGE"
     gene            complement(3238..3978)
                     /gene="hemD"
                     /locus_tag="OO230_003615"
     CDS             complement(3238..3978)
                     /gene="hemD"
                     /locus_tag="OO230_003615"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5693231.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWYFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3975..4916)
                     /gene="hemC"
                     /locus_tag="OO230_003616"
     CDS             complement(3975..4916)
                     /gene="hemC"
                     /locus_tag="OO230_003616"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5693232.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5303..7849
                     /gene="cyaA"
                     /locus_tag="OO230_003617"
     CDS             5303..7849
                     /gene="cyaA"
                     /locus_tag="OO230_003617"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5693233.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7889..8209)
                     /gene="cyaY"
                     /locus_tag="OO230_003618"
     CDS             complement(7889..8209)
                     /gene="cyaY"
                     /locus_tag="OO230_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5693234.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8257..>8678)
                     /locus_tag="OO230_003619"
     CDS             complement(8257..>8678)
                     /locus_tag="OO230_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5693235.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1974 a         2241 c         2240 g         2223 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtt tagcttccgt
     2101 tgtccccgct gccggttgtg ccagcaggtt acgcacgcga gtctgcatca gtttttccag
     2161 catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct ggcttaactg
     2221 gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt aagcacgtac
     2281 ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctgatgac gcggtacagc
     2341 ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat cctgatttgg
     2401 cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt tgtccataaa
     2461 gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg aaagctcttc
     2521 accgtctgaa tccatcggcg aaccatcgct gtcattatcg gccagacgca ggttatctac
     2581 ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct gcgatactgc
     2641 agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac tcgggtcatt
     2701 catatccgcc aggctggcgt ctgcactttt cagcaacgct gcagaggtcg tgacgtcctg
     2761 atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag ccagcagcca
     2821 ggttttagca tcgctgccgg aaatggtggc gatcttttgt tggacttcat ccaactgttt
     2881 tgccagcgtt tcttgctgac gattcgcctg cttaagctgc gcagcttgtt gcttaataat
     2941 gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca gttggttagc
     3001 cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc catacaaacc
     3061 gacacccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg cagtattgtt
     3121 cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct ccctggtctc
     3181 ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt atgagagtta
     3241 ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa tgtcttgcca
     3301 gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc gacagtgtag
     3361 taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca acatttcacc
     3421 gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt cttctgcacc
     3481 atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag caccgcgcac
     3541 cgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca gcgcacgttt
     3601 gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt cccgatcctg
     3661 tgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg tgcgtccaat
     3721 ggcgaaataa gtaggtagtt ggggccattt acgatcttgc tgatgcagct gtgattgggc
     3781 aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca gcgctgccag
     3841 ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg gaaaatacca
     3901 ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg gagacgggcg
     3961 ggtgaccagg atactcatgc cggggcctct ccgttataga cttcagcgag gatctcgcgc
     4021 gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc atcttgcggc
     4081 gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac cagcgcacat
     4141 agccagattt cgccatcaat aagctcggcg tagctaccaa ttggcacctg acatccgcct
     4201 tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc gtggtgattc
     4261 agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc ggcattcaat accgactgca
     4321 ccttgtccta ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat gcgggactcc
     4381 agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc gccgttatct
     4441 agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag atccgggcgg
     4501 cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact gcctgctggt
     4561 aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc ttcacgctcg
     4621 caaatagtga ccagccccag accttgcggg aattcaaccg gcacatcttt cattgaatgt
     4681 acggcgatat cagcgcgatt ctccaggagc gcgacttcca gctcttttac aaataagcct
     4741 tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt caccatcggt
     4801 accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac atagtgtgcc
     4861 tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc taacatgctt
     4921 gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga tgtcagtgtt
     4981 gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg gatcctgaca
     5041 ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta cacttgtatg
     5101 tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt tttagaccat
     5161 tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta cctttgacat
     5221 acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca gtcatgacgg
     5281 gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac agagactgga
     5341 tgccataaat caattgcgtg tggatcgcgc gcttgctgct atggggcctg cattccaaca
     5401 ggtctacagt ctactgccga cattgttgca ctatcaccat ccgcgaatgc cgggttacct
     5461 tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc aacgccacta
     5521 cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga aaggtgagct
     5581 tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa gttgttcctc
     5641 tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc gccagttact
     5701 acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtgg aagtcagctt
     5761 cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcctgg ggggcgaaga
     5821 ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtactg ccgtgcgtct
     5881 cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc attacgacga
     5941 ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc tggatctcgg
     6001 tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc agctctacaa
     6061 gagtatcgat tccccataca aagcggtgct gaaaacactg ctgctggaag cctattcctg
     6121 ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc acgacggcga
     6181 gattgtatcg tttggcctcg atccatactg catgatgctg gagcgtgtta ctgaatacct
     6241 gacggcgatt gaagatttca cccgtctgga tttagtacgt cgctgcttct atttaaaagt
     6301 gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag tgttgagcca
     6361 gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata accgtgctaa
     6421 ctggaagatt gatcaggtgc gtgaggcgca caacgagctg ctcgacgcga tgatgcagag
     6481 ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg ccagtccgca
     6541 ggatatcggc gtactgacgc gtaaactgta tgccgcgttt gaagcattgc caggtaaagt
     6601 gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga cctttattta
     6661 tgtgccgccg ggccgggcta accgttcagg ttggtatctg tataaccgcg cgccaaatat
     6721 tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaata aactggtggc
     6781 gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta aaggtaacgg
     6841 cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc atttcccgct
     6901 gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagattc gccatctggc
     6961 gattatcgtt aacctggaat atgacccgac agctgcgttc cgcaatcagg tggtgcattt
     7021 tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc tggtaggtag
     7081 cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact tcaacggcga
     7141 gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg acgccgcacc
     7201 gccagatagc gtggaagtct tctgttatag ccagcatctg cgcggcttaa tccgtactcg
     7261 cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc gccaggaaac
     7321 cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct tcgaacgcct
     7381 gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta tttcgcataa
     7441 caaactgcac ggcttgtcag tgcaggttga aaccaatcac gtcaaattac cggcggtggt
     7501 ggacggcttt gccagcgaag ggatcatcca gttctttttc gaagaaacgc aagacgagaa
     7561 tggctttaat atctacattc tcgacgaaag caaccgggtt gaggtgtatc accactgcga
     7621 aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc tactcgtcat cgcacgaccg
     7681 cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc agattgtgaa
     7741 ggttgatggt cgcgaacagg tgattccgtt ccgaacaaaa tcgattggca acctgccgcc
     7801 cgccaatcag gatcacgata cgccgctatt acagcagtat ttttcgtgat gagcgtgccg
     7861 gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca cccgcctgtt
     7921 gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg caaatccatt
     7981 catcgccttt caggtcaaaa tggtagccgc cctgtttggt tgccagccac acctggtgca
     8041 gcggctcctg gcggttaata atgattttgc tgccgttttc aaaggtaatg gtcagtacgc
     8101 cgccgttgat ttcgcagtcg atatcgctgt cgccatccct gtcgtccaga cgctcttcaa
     8161 tggtcagcca cagttgatca gccaggcgat gaaattcact gtcgttcatt gttgtatcct
     8221 gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag tttgcacctc
     8281 tgctgctgcg ttccggcatg attcatccgt caccggaata atgatgtctc tgttttgaac
     8341 ttcacgtacg ttctcctctc tgacacgagt tttcgccggg cagggggagg gcgtaatcct
     8401 tacctctgta tcggtccatg tataaacatt gcgatcgtca taagcgaagc gatcgttttt
     8461 agttagcgaa ccgttaactg gaataatttg caagctcttt gaccgtaatt ggcggataaa
     8521 gtaagcatgc tgtttgccgc tattaaatga gcgatcaatg accaggtctg gcgtatccag
     8581 cgggattatc tcaagctggt cttcaccaaa tgtcgataat tttgttacat acagccgatc
     8641 tttatattgc gcaatattgc cagtaatcag atggaatt
//
LOCUS       DAJTPG010000144         8542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTPG010000144 DAJTPG010000000
VERSION     DAJTPG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..723
                     /locus_tag="OO230_003620"
     CDS             <1..723
                     /locus_tag="OO230_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5693236.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRT
                     ALRAVRDTVVSVARAVWNWIFF"
     gene            1057..4179
                     /gene="ag43"
                     /locus_tag="OO230_003621"
     CDS             1057..4179
                     /gene="ag43"
                     /locus_tag="OO230_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5693237.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            4300..6816
                     /locus_tag="OO230_003622"
     CDS             4300..6816
                     /locus_tag="OO230_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693238.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            6892..7347
                     /locus_tag="OO230_003623"
     CDS             6892..7347
                     /locus_tag="OO230_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693239.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(7466..7677)
                     /locus_tag="OO230_003624"
                     /pseudo
     CDS             complement(7466..7677)
                     /locus_tag="OO230_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7758..>8542
                     /locus_tag="OO230_003625"
     CDS             7758..>8542
                     /locus_tag="OO230_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5693240.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT         2027 a         2254 c         2417 g         1844 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcacaccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcagcgctt ccgtctgagt ggtcacggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaagccct gtaccgtgac
      181 attctgcctg aactggacct ggtgctgtgg ctgattaaag ccgatgaccg ggccctgtct
      241 gtggatgagt atttctggcg acacatcctg cagtgcggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ctgacaaaac ggaaccctgc catgaatggg atatggcagg tattcagcct
      361 tctcccgcac aggaacagaa cattcgtgag aaaacggagg cggtattccg tctgttccgg
      421 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      481 agtgcgctca tgacagcgct tcccgaccat gccgccagcc ccctgatgac ccgactgcag
      541 gacgagctgc gcacggagtc tgtccgctct caggcccgtg aacagtttac cggtgcggtg
      601 gaccggatat ttgacacagc ggagagcgtc tgtgttgcct ctgttgtccg tacggccctg
      661 cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg gatcttcttc
      721 tgaacctgtt gtggatgatg tcctccctgc ctctgagtct gctcacaaaa gcgctgtttt
      781 cgttactgtc tctcttgtcc gtgcaataga ctgataatag aataaaacga tcgataccta
      841 ttttaccgat cgttaatatc gatcgttatg ctaataataa ctcctgttag caacgtgcgc
      901 agatacacac agacatgaga ttcagggaac aacagagcca cacgtcagaa acttccgtca
      961 gccggacctc cggcactgta accctttacc tgccggtatc cacatctgtg gataccggct
     1021 tttttattca ccctcactct gattaaggaa atgctgatga aacgacatct gaatacctgc
     1081 tacaggctgg tatggaatca cattacgggc gctttcgtgg ttgcctccga actggcccgc
     1141 gcacagggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcggt cacgtcactc
     1201 ccggtgctgg ctgctgacat cgttgtgcac ccgggtgaaa cagtgaatgg cggaacactg
     1261 gtaaaccatg acaaccagtt tgtatccgga acagctgatg gcgtgactgt cagtaccggg
     1321 cttgagctgg ggccggacag tgacgaaaac accggcgggc aatggataaa agcgggtggc
     1381 acaggcagaa acaccactgt caccgcaaat ggtcgtcaga ttgtgcaggc aggaggaact
     1441 gccagtgata cggttattcg tgatggcgga gggcagagcc ttaacggact ggcggtgaac
     1501 accacgctgg ataacagagg tgagcagtgg gtacacgggg gagggaaagc tgccggtaca
     1561 attattaacc aggatggtta ccagaccata aaacatggcg gactggcaac cggaaccatc
     1621 gtcaacaccg gtgcagaagg tggtccggag tctgaaaatg tgtccagcgg tcagatggtc
     1681 ggagggacgg ctgaatccac caccatcaat aaaaatggcc ggcaggttat ctggtcttcg
     1741 gggatggcac gggacaccct catttacgcc ggtggtgacc agacggtaca cggagaggca
     1801 cataacaccc gactggaggg gggtaaccag tatgtacaca acggtggcac ggcaacagag
     1861 acgctgataa accgtgatgg ctggcaggtg attaaggaag gaggaactgc cgcgcatacc
     1921 accatcaacc agaaaggaaa gctgcaggtg aatgccggcg gtaaagcgtc tgatgtcacc
     1981 cagaacacgg gcggtgcact ggttaccagt acagctgcaa ccgtcaccgg cacaaaccgc
     2041 ctgggagcat tctctgttgt ggagggtaaa gctgataatg tcgtactgga aaatggcgga
     2101 cgcctggatg tgctgaccgg acacacagcc actaataccc gcgtggatga tggcggaacg
     2161 ctggatgtcc gcaacggtgg caccgccacc accgtatcca tgggaaatgg cggtgtactg
     2221 ctggccgatt ccggtgccgc tgtcagtggt acccggagcg acggaaaggc attcagtatc
     2281 ggaggcggtc aggcggatgc cctgatgctg gaaaaaggca gttcattcac gctgaacgcc
     2341 ggtgatacgg ccacggatac cacggtaaat ggcggactgt tcaccgccag gggcggcaca
     2401 ctggcgggca ccaccacgct gaataacggc gccatactta ccctttccgg gaagacggtg
     2461 aacaacgata ccctgaccat ccgtgaaggc gatgcactcc tgcagggagg cgctctcacc
     2521 ggtaacggca gcgtggaaaa atcaggaagt ggcacactca ctgtcagcaa caccacactc
     2581 acccagaaag ccgtcaacct gaatgaaggc acgctgacgc tgaacgacag taccgtcacc
     2641 acggatgtca ttgctcagcg cggtacagcc ctgaagctga ccggcagcac tgtgctgaac
     2701 ggtgccattg accccacgaa tgtcactctc gcctctggtg ccacctggaa tattcccgat
     2761 aacgccacgg tgcagtcggt ggtggatgac ctcagccatg ccggacagat tcatttcacc
     2821 tccacccgca cagggaagtt cgtaccggca accctgaaag tgaaaaacct gaacggacag
     2881 aatggcacca tcagcctgcg tgtacgcccg gatatggcac agaacaatgc tgacagactg
     2941 gtcattgacg gtggcagggc aaccggaaaa accatcctga acctggtgaa cgccggcaac
     3001 agtgcgtcgg ggctggcgac cagcggtaag ggtattcagg tggtggaagc cattaacggt
     3061 gccaccacgg aggaaggggc ctttatccag gggaataagc tgcaggccgg tgcctttaac
     3121 tactccctca accgggacag tgatgagagc tggtatctgc gcagtgaaaa tgcttatcgt
     3181 gcagaagtcc ccctgtatgc ctccatgctg acacaggcaa tggactatga ccggattctg
     3241 gcaggctccc gcagccatca gaccggtgta agcggtgaaa ataacagcgt ccgtctcagc
     3301 attcagggcg gtcatctcgg tcacgataac aacggcggta ttgcccgtgg agccacgccg
     3361 gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg acctgctcag aacagaggtt
     3421 gccggtatgt ctgtgaccgc gggggtatat ggtgctgctg gccattcttc cgttgatgtt
     3481 aaggatgatg acggctcccg tgccggcacg gtccgggatg atgccggcag cctgggcgga
     3541 tacctgaatc tgatacacaa cgcctccggc ctgtgggctg acattgtggc ccagggaacc
     3601 cgccacagca tgaaagcctc atcggacaat aacgacttcc gcgtccgggg ctggggctgg
     3661 ctgggttcgc tggaaaccgg tctgcccttc agtatcactg acaatctgat gctggagccg
     3721 caactgcagt acacctggca gggactctcc ctggatgacg gccaggataa cgccagttat
     3781 gtgaagttcg ggcatggcag tgcacaacat gtgcgtgccg gcttccgtct gggcagccac
     3841 cacgatatga actttggtaa aggcacctca tcccgtgaca ccctgcgcgg cagtgccaaa
     3901 cacagtgtgc gtgaactgcc ggtgaactgg tgggtacagc cttctgttat ccgcaccttc
     3961 agctcccggg gggacatgag catgggtaca gccgcagccg gcagtaacat gacgttctca
     4021 ccgtcacaga atggtacgtc actggacctg caggccggac tggaagcccg tgtccgggaa
     4081 aatatcaccc tgggcgttca ggccagttat gcccacagca tcaacggcag cagcgctgaa
     4141 ggttataaca gtcaggccac actgaatgta accttctgat aattctgcat tgcctctctg
     4201 tggtcccggt catgatgacc gggacccgga cgggggaaaa cgtttcagtg ccacattcac
     4261 tggcattcac aataacatga tattcatcac ggagtgacta tgttacagat agtcggcgcg
     4321 ctgattctgc tgatcgcagg atttgccatt cttcgccttt tgttcagagc attaaccagc
     4381 acagcgtctg cgctggcagg gttcatattg ctgtgtctgt tcggtccggc tttactggct
     4441 ggctatatca ctgaacgcat aacccggtta ttccatattc gctggctggc aggcgtattt
     4501 ctgacgattg ccggaatgat catcagcttc atgtggggac ttgatggtaa acatatcgca
     4561 ctggaggctc atacttttga ctctgtaaaa tttattctga ccaccgctct cgccgctggt
     4621 ctgctggctc ttcccgtgca gataagaacc attcagcaga acgggctcac acctgaagat
     4681 atcagcaagg aaattaacgg gtattactgc tgtttttata ctgctttttt ccttatggcg
     4741 tgttctgcat acgcaccatt gatcgcattg cagttcgata tttcaccctc actgatgtgg
     4801 tggggcgggt tgttgtactg gctggctgca ttagtgacgc tgctatgggc ggccagccag
     4861 atccaggcgc tgaaaagact gaccagtgcc atcagccaga cactggaaga acaaccggtg
     4921 ctcaacagta aatcgtggct gagcagtttg caaaacgatt acagccttcc tgaaacgctg
     4981 acggagcgca tctggctgac actcatttca caacggattt cccggggaga actgagggaa
     5041 tttgaactgg cagacggaaa ctggttactg aacaatgcct ggtatgaaag aaacatggca
     5101 gggtttaacg aacagttgaa agagaacctg tcattcacac ctgatgaact gaaaacgctc
     5161 ttccggaacc gcctgaattt atcaccggaa gcgaatgacg attttctcga tcgttgcctg
     5221 gacggcggtg actggtaccc cttttcagaa ggccgccgtt ttgtatcatt ccaccacgtg
     5281 gatgagcttc gtatctgtgc ctcctgcggg ctgacagaag tacatcatgc cccggaaaat
     5341 cataagccgg ctccggaatg gtactgctcc tctctttgtc acgaaacaga aacactgtgt
     5401 caggacattt atgaacgttc ttacaccggt tttatttccg atgcaacggc gaatggtctg
     5461 attctcatga aactgccgga aacctggagt acaaatgaga aaatgtttgc ttccggaggg
     5521 cagggacatg ggtttgccgc tgaacgggga aaccatattg tcgacagagt ccgtctgaaa
     5581 aacgcacgga tcctcggtga taataatgcc agaaatggag cagacagact ggtcagcgga
     5641 acagaaatcc agacgaaata ttgttcaact gcagcccgta gcgtcggtgc ggcattcgac
     5701 ggacaaaacg gacagtatcg ttacatggga aatcatggtc ccatgcaact ggaagtcccc
     5761 cgtgatcagt atgccggcgc tgtggaaacc atgaagaata agatccgcga aggtaaagta
     5821 cccggtgtaa ccgatcccgc agaagcgtcc cggctgattc gtcggggaca tctgacttat
     5881 acccaggccc gtaatatcac ccggttcggg accatcgaat cggtcactta tgatattgcc
     5941 gaggggtcgg ttgtcagtct ggcggccgga gggatcagtt ttgccctgac ggcatcggtc
     6001 ttctggctca gcaccggcga tcgcgatgct gccctgcaga cagctgctgt ccaggcagga
     6061 aaaaccttca cccgcacact ggctgtctac gtcacaaccc agcaacttca ccggctcact
     6121 gttgttcagg gtatgctgaa gcatattgat ttttcgacgg ccagcccgac tgtccggcag
     6181 gcgcttcaga aggggaccgg tgcaggaaat atcagtgccc tgaacaaagt gatgaagggt
     6241 acgctggtga catctctggc actggtagct gtcacaacca gccctgacat gatcaaaatg
     6301 ttgcggggac ggatctccgg tgcgcagttc atcaggaatc ttgccgtggc atcttccggt
     6361 gtggcaggtg gtgctgtcgg gtcagtggcg ggcgggatat tgttcagtcc actgggacca
     6421 tttggtgcac tgacagggcg tgtggttggc ggtgttctgg ggggaatgat tgcctccgct
     6481 gtatcaggaa aaattgccgg agcgctggtt gaagaagatc gcgtcaaaat tctggcaatg
     6541 attcaggagc aggtgacatg gcttgccggc agtttcctgc tgaccggaca tgagattgaa
     6601 aatctgaacg cgaatctggc ccgtgttatc gatcagaatg ctctggagat cattttcgcc
     6661 gccggtatac aacaacgggc cgcgaccaat atgttaatca aaccactggt ggtcagtatc
     6721 atcaggcaac gccccgtcat ggaatatgat gcatcccatc tcggcaagat ggttaaccgg
     6781 ctggaagaag cattcccccc ggagctcccg gcataacgtg tgttctgctc cttttaggtt
     6841 ttatcacctg atttgccagt gtcatcgtca tgactcagag aggtaaatac catgattcac
     6901 ctgttcaaaa cctgcatgat taccgccttc attctggggt taacgtggtc tgccccactc
     6961 cgggcacagg atcaacgtta catcagtata cgcaatacag atacgatatg gctcccggga
     7021 aatatttgtg cttaccagtt ccggctggat aatggcggaa acggtgaagg atttggcccc
     7081 ctcaccatca ctctgcaact caaagacaaa tatggtcaga cgctggtgac cagaaaaatg
     7141 gaaacggaag cttttggtga cagtaatgcc acgcgaacca cagacgcatt tctggaaacg
     7201 gagtgcgtgg aaaatgtcgc cacaaccgaa atcattaaag caactgaaga aagtaacggc
     7261 catcgtgtca gtctgccgtt atcggttttc gatccccagg actaccatcc actgctgatt
     7321 accgtttccg gaaaaaatgt taactgatcc ctgaagtcag tttcatgctt cagcctctgc
     7381 cacaacgtat ggcagaggct atttatcagg agaaaccaga tatgccgggt tacacacaga
     7441 gtatgtcctg gctgaaggat ctttctcata cgggcaggcg gttgctgtca taaccgctta
     7501 ccgcaatgtg tttatccagg atgacccggg aatgcatttc cgtcgggtta tccgcaacgc
     7561 ggaaggtcag cgacgctgac gcagccggaa ctctgaagct gatgccggaa aacagcttaa
     7621 tgcgtggctc gccagtggag ggcttctcag acaataaact tcattgattt catccatcag
     7681 gccgcgtctt ctccgggaga cgcggctttt ttcatttgtt gcaccagtaa atcttaacca
     7741 ccgataagga gcaaagtatg caattagtca gtcgttttgg ttatgcgaac cagatacgcc
     7801 gtgaccgtcc gctgacacat gaagagctga tgcaccatgt gccggggatt ttcggggaag
     7861 agaaacacac gtcccgaagc cagaactata cgtacatccc caccatcacc gtactggaaa
     7921 gtctgcagcg ggaaggcttc cagccctttt tcgcctgcca gacccgtgtg cgcgacccgg
     7981 gccgccgggg atacacaaaa cacatgctgc gtctgcggcg ggccggagag ataaacggag
     8041 aacatgtccc tgaaattatt ctgctcaact cccatgacgg tacctccagc taccagatgc
     8101 tgccgggtta cttcagattc gtctgccaga acgggtgtgt ctgtggtcag tctctggggg
     8161 aagtgcgtgt tccgcaccgg ggaaacgtgg tggagaaagt tattgaaggg gcttacgaag
     8221 tggtgggcgt gtttgaccgg attgaggaga aacgtgatgc catgcagtcg ctggtcctgc
     8281 cgccaccggc acgccaggcg ctggcacagg cggcactgac ttaccgttat ggtgacgaac
     8341 atcagcccgt caccaccgcc gacattctga cgccacgacg ccgggaggat tacggtaagg
     8401 acctgtggag tacttatcag accatccagg agaatatgct gaaaggcggg atttccggcc
     8461 gcagtgcaaa aggaaaacgt atccacaccc gtgccattca caacatcgac accgatatta
     8521 agctcaaccg cgcattgtgg gt
//
LOCUS       DAJTPG010000145         8468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTPG010000145 DAJTPG010000000
VERSION     DAJTPG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            74..398
                     /locus_tag="OO230_003626"
                     /pseudo
     CDS             74..398
                     /locus_tag="OO230_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(428..3340)
                     /locus_tag="OO230_003627"
     CDS             complement(428..3340)
                     /locus_tag="OO230_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693241.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3343..3459
                     /locus_tag="OO230_003628"
     CDS             3343..3459
                     /locus_tag="OO230_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5693242.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3823..4665
                     /locus_tag="OO230_003629"
     CDS             3823..4665
                     /locus_tag="OO230_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5693243.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4668..5756
                     /gene="capU"
                     /locus_tag="OO230_003630"
     CDS             4668..5756
                     /gene="capU"
                     /locus_tag="OO230_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5693244.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5761..6711
                     /gene="virK"
                     /locus_tag="OO230_003631"
     CDS             5761..6711
                     /gene="virK"
                     /locus_tag="OO230_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5693245.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6776..7720
                     /gene="lpxM"
                     /locus_tag="OO230_003632"
                     /gene_synonym="msbB"
     CDS             6776..7720
                     /gene="lpxM"
                     /locus_tag="OO230_003632"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5693246.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7874..>8468)
                     /locus_tag="OO230_003633"
     CDS             complement(7874..>8468)
                     /locus_tag="OO230_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5693247.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT         2237 a         1921 c         2008 g         2302 t
ORIGIN      
        1 acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg aacagtccac
       61 aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc agttccgtca
      121 gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca cgcctgacgg
      181 atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca agcctgcgcc
      241 gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac cgcgcacatg
      301 gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt ccgaaccaga
      361 agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat cctgtcagca
      421 cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat ccgacagact
      481 tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat tccggtgaca
      541 tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc aactcataaa
      601 ccgcgcgcca cacatcaccc ttccttattt taagccccgg gttaagaata tactgtccgt
      661 ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact tcatgttgag
      721 agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaac gcttcaggaa
      781 gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac
      841 ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt gccatctgcc
      901 ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca ggtttcagca
      961 gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc gccgctgaca
     1021 gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct gcaccacgct
     1081 ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc agcggagtaa
     1141 gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac acggcaaccg
     1201 ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga gtcttcgttc
     1261 tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc gggaacaggg
     1321 aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct gtacctgcga
     1381 ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat ggcatctcag
     1441 atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa
     1501 gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc agattcagag
     1561 gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc tgctgttcaa
     1621 caagatacac attgtgttta gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt
     1681 tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg gcctcgccct
     1741 caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta
     1801 ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga accgaaaaga
     1861 ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc atcacaatga
     1921 ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc tcttccctat
     1981 cgtccagcaa ccggatggtc ccttctgcct gaccgcagct tctgacaaaa tggtgacttt
     2041 cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta gccagtgcag
     2101 tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg tcgtttccct
     2161 gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc ggatgaacta
     2221 tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt ttcagtacca
     2281 gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca tcataacggg
     2341 gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga tgcgactgtt
     2401 tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc tccatgccat
     2461 agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc ccattcccgg
     2521 aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca gcccccagtg
     2581 cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc acctccctgc
     2641 cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca atattctgta
     2701 cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc agaaatgagg
     2761 aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt accagcgccg
     2821 tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg tacagtgcct
     2881 gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca tgagacggat
     2941 tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt tgttgttgta
     3001 agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta taggcatgat
     3061 tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac agcgaaaaca
     3121 gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt ttacgaacat
     3181 cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa cccgggatcg
     3241 tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc cgtcgtgcaa
     3301 aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg aatacccccc
     3361 gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga aggtggggtg
     3421 gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg tcatgatgca
     3481 ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa ctctgcgctg
     3541 aaattccttt ttttcattgg agtacctgtg ttgttctgag gtgaacatat cacctctgtt
     3601 caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt taaaagatat
     3661 gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat atcaaatatg
     3721 ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca gctttattgg
     3781 gttttccttt tgtctctgct tgctaggtgc cagaaacttt aaatgttaaa cgaagggggc
     3841 attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag tcattgcccc
     3901 ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt ggctgaaaac
     3961 aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg aaaactcccc
     4021 cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt tcaggtatat
     4081 cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg ttttattggg
     4141 aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg cgaacatcaa
     4201 atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa cgaaatgttg
     4261 caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg ggataaaaag
     4321 ttcagttcac gagaagaaca gtgtaaacat cttcggcagg acttgttgag cgggagggaa
     4381 tatttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga aggttattat
     4441 aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata tacaacagaa
     4501 agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag caccaaagaa
     4561 cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacccc gtttttttca
     4621 tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg aatatactat
     4681 ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct caaatgaagg
     4741 ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc aaaattgctt
     4801 ttgaagccag taaattgggg attgatatca cattcgcgtt atttcgaaac agtcttcaca
     4861 tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat gcaatcgttt
     4921 gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact aggaaacatc
     4981 cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt ttttcaataa
     5041 atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat ctggagcagg
     5101 aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag aaattatacg
     5161 ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc cgaaggggat
     5221 gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa tttatgttga
     5281 atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt gtggggtctg
     5341 gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc atgcatgatg
     5401 atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt gccagtctgg
     5461 tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca tcggcatttt
     5521 ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag aataaccaga
     5581 ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg aatgattttt
     5641 ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat atagaagagc
     5701 ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac aagtaacaat
     5761 atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt tttttcctca
     5821 gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat tttgcgatgt
     5881 gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt gcagtgcatt
     5941 gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc ttatttgcat
     6001 aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg ctttgtgcaa
     6061 aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc attagtccaa
     6121 ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg ttttgaccgg
     6181 gaaggagagt tgatgttgtc attgtttttc aataaaatag tgattgcccg gttaacattt
     6241 tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca gggagccccc
     6301 aaaaataccg gacctgatat tattcggtgc gcaacccggg cctgttatgg gcttttccca
     6361 aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt ctctgagtgt
     6421 ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta tcagaaacgc
     6481 aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa aacctgtggt
     6541 gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat agctagtaaa
     6601 aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga aactgctatc
     6661 cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta aggtttgatg
     6721 tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact aaataatgaa
     6781 aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc ctgtttactg
     6841 gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc cttcattcag
     6901 agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa aagctcgtcg
     6961 cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag aacgggaaat
     7021 aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg cagaactggc
     7081 aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg aaattcttga
     7141 ggaaattcgg cacaataaca gaaatgtgat ttttctggtt ccccatggct ggagcgtgga
     7201 tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt ttcatcagca
     7261 acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg gggggcgctt
     7321 acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg gatactgggg
     7381 gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg atttttttgc
     7441 gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc aggctatgat
     7501 tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg aagttcggcc
     7561 accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac aaatgaacaa
     7621 aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt taaaattgtt
     7681 aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag tacaggtatt
     7741 tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg tgtgttgccc
     7801 gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatgc ctaaatagct
     7861 gcgcttaata ccgctacact tttgccagcc catgtttgcc tccggggaat gggctgacgg
     7921 tttccataaa atggcgaact tttttcaaca gttgccacat tgagcggcac tgatgattac
     7981 gcgttattgt gtcgtgaagt gcctgccata gccgttcaac atgattcacc catggcgagt
     8041 aaaccggctg ataaatgccc ctgaacttcg ggttctcctt cagccagctc tgtgtttccc
     8101 ggcttttgtg gataatgtag ttgtccacga tcagcgtgat ggttttcgca cgacggtatg
     8161 tcgctttaag ccgcttcagc aggctgatga acagcgccga acttttgctg ttgccgccca
     8221 catagctgac tttacctgtc ccgctgtgca gcgctccggc cagataatat ttttcattct
     8281 gtcccggcgt gaccacccgt ttttgctgtc cgcgcagttg ccagtccgca ccgattttgg
     8341 gatgaagatg gatatccact tcatcttcat aaaagaccgg atgctctgcg ctgcattcgt
     8401 ccagtgcttt atggattgct gccatctttt catctttatg cgggtcacgg atacgcagag
     8461 ttggcgca
//
LOCUS       DAJTPG010000146         8332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTPG010000146 DAJTPG010000000
VERSION     DAJTPG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..158
                     /locus_tag="OO230_003634"
     CDS             <1..158
                     /locus_tag="OO230_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693248.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO230_003635"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO230_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5693249.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO230_003636"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO230_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5693250.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1245
                     /locus_tag="OO230_003637"
     CDS             874..1245
                     /locus_tag="OO230_003637"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5693251.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1266..6127
                     /gene="traI"
                     /locus_tag="OO230_003638"
                     /pseudo
     CDS             1266..6127
                     /gene="traI"
                     /locus_tag="OO230_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6147..6893
                     /gene="traX"
                     /locus_tag="OO230_003639"
     CDS             6147..6893
                     /gene="traX"
                     /locus_tag="OO230_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5693252.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6948..7508
                     /locus_tag="OO230_003640"
     CDS             6948..7508
                     /locus_tag="OO230_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5693253.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7639..7851
                     /locus_tag="OO230_003641"
     CDS             7639..7851
                     /locus_tag="OO230_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5693254.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8095..>8332
                     /locus_tag="OO230_003642"
     CDS             8095..>8332
                     /locus_tag="OO230_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5693255.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1912 a         2085 c         2671 g         1664 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag
     1261 aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc
     1321 aggaaccaca gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat
     1381 ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta
     1441 atcagattgc ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg
     1501 gctatgaaac tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg
     1561 aggccttttc cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga
     1621 aatcccggga tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag
     1681 tcagaatggc cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc
     1741 gtgacgcagc ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg
     1801 acgggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag
     1861 tgcagtttat gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg
     1921 gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc
     1981 ccctcgatcg tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag
     2041 tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa
     2101 gtgtcccccg gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt
     2161 cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac
     2221 tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct
     2281 cgcagatgaa cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc
     2341 agttgctgga aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa
     2401 aactctccct gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg
     2461 tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg
     2521 atgccggggt gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca
     2581 gtgaaccgga ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga
     2641 agagagcgtg gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg
     2701 cagtgagctg aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc
     2761 accggtctgg ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt
     2821 gatggagcag tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac
     2881 ggcgcagagt cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat
     2941 ttcttccctg gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg
     3001 tgagcgtctg agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct
     3061 gcaggtggca tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc
     3121 ggccaccctg cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt
     3181 ggaaacgccc gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat
     3241 ggcaatggac aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta
     3301 ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt
     3361 ggtttctgag cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct
     3421 gcagaaagcc gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct
     3481 ggaaagtaaa aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc
     3541 tgcagaagga accggttttg ctgacctggg aggggaaatc aatgtgcaga taaaacgggg
     3601 tgatttactg tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc
     3661 gtcgtatgag gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac
     3721 gccgctgatg gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc
     3781 cgccacccgt atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg
     3841 tgtgggtaag accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag
     3901 tgaacgtccc cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag
     3961 cgccggcgtg gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg
     4021 cagcggagaa actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt
     4081 gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt
     4141 ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca
     4201 gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga
     4261 actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct
     4321 tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc
     4381 cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat
     4441 gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga
     4501 ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga
     4561 ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg
     4621 gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg
     4681 tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca
     4741 ccggattgcc ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa
     4801 cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga
     4861 caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg
     4921 ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc
     4981 tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga
     5041 cctggcctat gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc
     5101 gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc
     5161 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     5221 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     5281 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     5341 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     5401 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     5461 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     5521 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     5581 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     5641 ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa
     5701 tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     5761 gggagccgga aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     5821 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     5881 caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga
     5941 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga
     6001 accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg
     6061 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     6121 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     6181 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     6241 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     6301 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     6361 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     6421 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt
     6481 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     6541 cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt
     6601 ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc
     6661 gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg
     6721 cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga
     6781 ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt
     6841 tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt
     6901 ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga
     6961 agcgaccggt actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga
     7021 aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg
     7081 agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
     7141 ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc
     7201 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
     7261 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
     7321 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
     7381 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc
     7441 gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
     7501 agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat
     7561 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     7621 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     7681 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     7741 gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt
     7801 gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     7861 cagccttcat tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa
     7921 tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa
     7981 gtcgattttt tatattttgt gattagtttc acaaaataag gtgttattca atgtgtgctg
     8041 cactattcag gggggctgaa atacaggtgg catttatctg atatggagaa taacatgagg
     8101 aaatatattc cactggcatt atttatcttt tcatggccgg tattgtctgc agatattcat
     8161 ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg
     8221 gtgcggatca ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg
     8281 tcaacagaca tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac at
//
LOCUS       DAJTPG010000147         8299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTPG010000147 DAJTPG010000000
VERSION     DAJTPG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(1..268)
                     /locus_tag="OO230_003643"
                     /pseudo
     CDS             complement(1..268)
                     /locus_tag="OO230_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(319..894)
                     /locus_tag="OO230_003644"
                     /pseudo
     CDS             complement(319..894)
                     /locus_tag="OO230_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            898..1298
                     /locus_tag="OO230_003645"
                     /pseudo
     CDS             898..1298
                     /locus_tag="OO230_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1701..2282)
                     /locus_tag="OO230_003646"
     CDS             complement(1701..2282)
                     /locus_tag="OO230_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693256.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2329..6186)
                     /gene="sigA"
                     /locus_tag="OO230_003647"
     CDS             complement(2329..6186)
                     /gene="sigA"
                     /locus_tag="OO230_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5693257.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(6547..7551)
                     /locus_tag="OO230_003648"
                     /pseudo
     CDS             complement(6547..7551)
                     /locus_tag="OO230_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            7536..7913
                     /locus_tag="OO230_003649"
                     /pseudo
     CDS             7536..7913
                     /locus_tag="OO230_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7952..>8299)
                     /locus_tag="OO230_003650"
     CDS             complement(7952..>8299)
                     /locus_tag="OO230_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5693258.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         2036 a         1953 c         1907 g         2403 t
ORIGIN      
        1 accagaccgc cgggggcatc tgtgaaccgg cgttacggtc atcacggaca tagacccaca
       61 gccgggcagt tcttgtttta ccactgcccg gctcctggac cggaacgggg atatcatccg
      121 catgaacttt accgggcatc agtacatact ggcgcaggac gtcatacagc ggctccagca
      181 gttcagcaac ggcaccggtc cagcgaccca gtgtggcacg gctcagctcc acgccctgac
      241 gacggtatat ttctgactgg cggtataact gtcaacgacg gatgaaaagt ggctcttccg
      301 caacctgggt ggagattttc atgccagtgg actcattacc ctctgcctga gtagttcaaa
      361 tcctgctcga ccatacatct ggcgtttcag catcttcttc aggcgattta catgaccttc
      421 gacaacacca ttactccagc gactactgat tgcctcacat atagctgcag catcagcttc
      481 catccccgcc gccacgcgcc gaaattctgc tgaaccgctt tcgtggactc gagtgaacca
      541 actgcttagc tgtgatttat tttgggtttt cagaatacgg tagaactcga gtgccagttg
      601 ctgcgctatt ttcagctcag gttctttttc acacatcaga ctaataaatc gggaagcata
      661 attttcttca tccctggtta ttctccaggg catcaaccag cggcttaccc gggagactga
      721 aggaagtctg acggcagtag taaccggtgg gttccagcct ttacgccatt tggcaaccgc
      781 atccctgacg atggtttcac tgcctgtaaa tccccgggcc accatttccc gccatatccg
      841 gctggcgtta taattaccac tttctcgttg ctcttttaac cactccctcc atggtgtcag
      901 cgaaaaacga aaccgctgtt gaaatccctg gaaagctggt tgcggaaaag atgaagaccc
      961 agtcgcgaca ctcagagttg gcgaaggcgt tcgcgtacgc acttaaccag tggccggcac
     1021 tgacgtacta tgcgaacgat ggctgggtgg aaatcgacaa caacatcgct gaaaatgcct
     1081 tgcgggcggt cagtctgggt cgtaaaaact tcctgttctt cggctctgac catggtggtg
     1141 agcggggagc gctactgtac agcctgatcg ggacgtgcaa actgaatgac gtggatccag
     1201 aaagctacct tcgccatgtg cttggcgtca tagcagactg gccggtcaac cgggtcagcg
     1261 aactgcttcc gtggcgcata gcactgccag ctgaataaca catccccgtc aatacggccc
     1321 tcgctgtacg cttactcatc acctgctatt aatccccgag tcagtacttc gcggcctgac
     1381 gcgatcaccc tactcagtac ttcagagccc gcctccggta cctgcctggc gatcgcccct
     1441 gtgcaacaaa ataaatcaac cgttatcaaa ccacgatcgt tcgtatgcgc cgccgatgac
     1501 gcgcgcctga aaaacgcgtc aatatgagtt ccccggaagc ttacgataat acgcttatga
     1561 acattacaac acgctcatgc ggaacctgga tgggggaagg aacggtgaag ctgttagcga
     1621 atgaccggcg tttatacaaa cgccggtcat gatgaatgat atttcagaat atacctcggg
     1681 ctaaaatacc gggacagaga tcaggcgtga ttaatctgca gcatttttaa taacagaagt
     1741 actgagctta ctttaccttt gatgatgtaa tagttatttc cgtcggtact catttttccc
     1801 tgaaataatg tcacggatgt ttcttcctgc tgatcctgca ggcgcagtcg taaggtcata
     1861 tttttaatga gctgagatcc gggtggcaag gggtatttgc tgtcagatac tgggcgactg
     1921 agtctgatgt ggcgcgtata cagcccgttt tcgtacaggg tggcctctgt tatggtgtca
     1981 gtgaccagca tgtgggagac aatgctgtat tcttttttaa agcacccacc agaaacggtg
     2041 attgtaatga taatggtctt ctcgatcgag atgtaaatac ccgtttcgtt atactgtccg
     2101 tatcggaacc atgagaactc agttgcattc cagccatgct ccagaatatg tgcatttttt
     2161 tgcctgatag tttctttttt tattcgctca tgtttaaggc ataacaggca gataaataat
     2221 gtcagaaaaa gaaagctaat aatgagagca agagcgatat agtctgtcac agggtaatac
     2281 atattctctc cggtagaaga agggccgcaa acgcggcccg ggctgttatc agaaagagta
     2341 acggaagttg gcgttgatcg cgttatccac gttgtattta ccaaatgccg atttctcaaa
     2401 ctcaagaccg aagcgaagat tatcgcgaat ttcggcgttg agaccaacat tcatgagaat
     2461 acgaccgtct ttttcacctt tgatacgttt ctcaccggac gcatcacgca gtacggtttc
     2521 accgttggca aacaggtcaa actggtagcc aaggccggcg cgggctgtga ctttccagtc
     2581 cttaccggag aaggatttac ccacatcaac accggtacgc ccaatcagcg gattaaagtc
     2641 cttatccttc atggtcacgc tcattccgcg atcctgccag tcgaaccgtt tacctgatac
     2701 ggccccataa accagttctg cctgcggctc aatccaggag tcttctgtta catgatagcg
     2761 gtagcctgct tcagcaccca catacaagga atgagaactg tagtcttttg ttccgagtcc
     2821 agcaaaggtc gccgagtact cattatcatg gtgaacatac ttactaatca ggtcgatata
     2881 ggcaccagag tcaaatatag cggaagcata cagacctgcc ccgacggact tcgtcttgcc
     2941 actgaatgca ttactgctgg catgactgtc ggtatacgtc atagtcagac cagtgaaaag
     3001 gtcaagtcca tccagctcat gttttttatc cacaccaatc tgcacatggg tgtagttgtc
     3061 actgtatcca ccagaagctg aacctgctcc gctcatgatg cgggcccatg caccggcctc
     3121 gccattggtg tcacgcagat cccccattcg tttattcagg ttgttgactt ctgtcaggaa
     3181 ggctttgtaa ttaccggaca tatacgattt tgctgcaccg gaagatgctg tattttcagc
     3241 caccttatat ccggtcaggg tccacgtggt atgttcctga tcctctttcg tgctgatcac
     3301 aggagatatg ttgctgaaac ctatggcctg agtactggct ttaaatatct ctttattgct
     3361 tccttttggg gctgttatca ggtcaacgtt taatcctgag ttattatttc ccactttctg
     3421 tatatcgacc gacagactgt tattgcttcc ggttaactga tttttaacaa tgagtttatc
     3481 cgattgattt acagactggc tcagatcagc ccgcataatc acagcagtat tagtggcatc
     3541 cagtgtgttt actgtcacag tatggaaacc cgaattctct atgttactaa acaacaaaag
     3601 actgtcgcgg gtttttaatg attgcagggc tgactgtccg tttactctcc ataaggtgtt
     3661 atacatgctg gctgaggctg caggagcatc aatacctccg gtataagaag tatcaaagcc
     3721 acccagcaaa cctgtagcca gcggtgtgtg tgtggttttg tccaggttat cttctttata
     3781 ctggtcaaca ccaaacagaa tagatgcttt tttgtctgat tttatattac cattgaccca
     3841 ggcataattt tgagccttta acgtggcacc atcttctgtc atattatatc catctgacat
     3901 atagattgcc gggctgtatt tattattttc tgtaggggtg cctgttaatg tcagggttcc
     3961 ggccagttca atagctttat ctacatacat ttttccctgg gcggtaacag tagctccttt
     4021 gttgataatc aggtctgtat taattaagga taatgcacca gtcatagtaa cagttgattt
     4081 atcaccaaca gttattttgc tgttttgagc actcagactg ccgttgaaac tactttcgcc
     4141 tgctgaatcc ccggagcgaa gctgttgtct gaatccaaaa ccttcgccag taatgtttct
     4201 tcctgagaac atgtcaatgt atgcagtttt atttcccaga acaatttgag agtttttagc
     4261 ctctatgtgt ccttttacat tagcatcccg gcctatactc agggttgcgt tttctaaatt
     4321 taatttccgg aaattaaatt tacgactttc ccagtcgggc tgggaaaaag aggctatctg
     4381 attgttggac ttatattccg tattgtaacg tttatttaca gtgctttcct ggtcctttat
     4441 ggcagcagaa tagtcttttt gacataaaaa aggaattgga cagttgttat tgccttcttt
     4501 aaatatggca tgttctgtcg catgtccttg taatgttaac tgtgcattcc ggacagagat
     4561 atcgtttttg atatcgactg agccatcaaa gatcagattg gcattattgt tatgttgctg
     4621 ggtagtattg ataatatgat ttatgcttat attacctgat acattcccat ggtacatata
     4681 tgcatcagta ttagtaagtg ataatgttga ttgcttcacg tttgagttcg ttacggttgt
     4741 tcccgcatct gttgctgcga ttttctgaaa tgactggtca tagccgttta gatccagtgt
     4801 tccgccattt ctggtaaaat agatttcgcc attaccgctt tcgcttaaag cgtctttggc
     4861 atttattttt accgtacctt tacctccggc catgtaaatt ttattgaagg ctttttcagc
     4921 actaaggatg acagtcccat tacctatttt aaggttattt ccctgtgcta tttttacatc
     4981 cagagtacca ctacctattt tatgcagatt atctccggca acgcccttaa cattccattc
     5041 aacggtggta ttattatcaa tatctatccc ggcaccttta taagacttat cttttccgta
     5101 cactttatat gtctggttct tatcgaatat aagcccgcca atgccaagat cggtatcctg
     5161 cttgagcgtt aatacccctc ctcctgagac agacaggtcc ttatctttta taatcgatga
     5221 taactcttgt tttttaccat ccagctgcat agtgtttgtg aaattctttt ttaaattgtt
     5281 taccagaata ttattaaata acgagtagtt tgtccaggct atattggtac tactgataaa
     5341 accaactgta ctggtaacac catggaaaac ccatttttgt tgttctttat catatagata
     5401 agaaccagaa ccactatcgc caaaaattgg tgagttttta aaaggggttc tgttggtata
     5461 aatgtaactc ttgtttttcc agtccagatt aaaaagactt gcgcttaaca agtcgggatt
     5521 atagttttgt ccaaacagat attgtttgcc gtttaatatc gttgaggttg ttccggcacc
     5581 tgctctgaag ccaattattt gttccttacc gttgtagttt acgccatatc ttttcatcgc
     5641 atcttcggga gaaagtgaga agtcggcact ctccgtaacc cctgttgttt ccacgacgaa
     5701 tttattcaga cgatgaaccg aaaagtcgcc agatgatact ctgttacttg ctttatatgc
     5761 tgtctgatcc catgactgtg ttgttattgc atgatgctgt gtaccgttat gagtcgcagt
     5821 aacactgtac gcacctccaa ttgatgttgt tgcacctttg ttggaaacag cagaaaaatc
     5881 aggtacaggt agttctggaa aatgaaatat ctttccatct ttcatcatta attgaacatt
     5941 tgttgcaccc ggtctgaact ctcctctgtt ttgagcaagg tcaagataat cgcgggtcca
     6001 tatattattt atatccagca ttgctgcaaa tgctgtttcg ccataacttc catatattga
     6061 ggataaaata ataccgagga taactttctt tcttgatgta ccgacactaa ctttccgggt
     6121 cagttcagaa acagcaacta atccacctgt aatatgacta tatttcagtg aataaatttt
     6181 attcatacgt gaactccgat atttctgagt tgtggttatg ggatagcatt taattacaac
     6241 ggggaactat cagtcatggt acgtaactga taagggtata atgataatca ctgtcagtta
     6301 tatttgtatg acagatgtaa caaaatgaaa aaaaagataa gcacaagtga ttgattggtt
     6361 agatattgat cacttggtgt ttgtattttg aattttgcga tcaaaatttg atctttgttg
     6421 tttccggtca atcctttaca ggttttctgt attatccggg ggagttaagt ctgtcgatgt
     6481 ccgacagact tttgatgatg gaaacaacat gcagcgataa tgtgcatgct gttttgcagg
     6541 tggaactcag aaggtgaact ggaactgtgc gaccgcaccg taagtgttgt ccgtgccgat
     6601 caggcctgca acatcaaggt gaaccacatc aaacggtgag atccccaccc cggccgtgaa
     6661 tgcactgccg tcgttaccgg ccagattctg acggtaaccg gcgcgcagct gtgcccattt
     6721 ccaggcatta aattctgtgc caatggcggc aaactgacgg ttgctgtcgg aggtgaaacc
     6781 gctggccggg gtcagatcca catcaaatgc agtggtgaac atcgcgttgt gccaggagac
     6841 accggccgtc acctgcgggc ggatcctgaa ggtttccgtg ataccgttca cttcttttgt
     6901 ctcaatacta cgggagatca ggttctgtgc gaccagtccc agcgtccagt tgtcatccag
     6961 gtccgtactg gcaccgatgt cggcgttgat gccatttttc gtgttgtggt agcggtcgcc
     7021 tttgaaggcg ctgctgtcat aatttctgac cagcacgttg tagttgaaca ggtcgacgcg
     7081 ctggaatttg ggggggaagc ccagagacca tttctgcccg gccgtttcca gttccgtagc
     7141 cagagcaata cccacatcag tgatcagtgc cgtgcgggca caggcctgtg atgtcaggcg
     7201 gcttttgtcc acctcctgcc cggtgctgtt tgccacctct tccaggtaag tgaggtcagc
     7261 atcagagact ttaccgtcga cactgacggt gccgtgtgat ttaaccatca gtgcggcaga
     7321 gaggcgatca ccgggaaggg ccgccacagc agaaacgccc agctgtgcgg cggcatgtgt
     7381 gttacggaag tcccggaggt gttctttcag gcgtgcggca gcctcaggaa caccgtggtt
     7441 actttcgatg gcgcggtcga aggccttcca ctcatcactg atccggtcgg cgttgtcggt
     7501 gatattatcc ggattggaga gctgcgcgcc gacggacgga agcaggatgc tgggctcatg
     7561 cgcgacgcaa aatccacgat gtatatatca gtactaaaag cgcgacggcg gaagaagctc
     7621 tgaaactaat cggtgaactg tacgccatag agcacgatat acgcgggtta ccggtgtctg
     7681 aacgcctggc ggtcaggcaa atgcagagta gaccgctcct gacttccctg tataagctga
     7741 tgcaggagaa agaacacacg ttatcgaaga aatgtcgtct gggagatgcg ttccggtata
     7801 tcaggaagca ctgggctgcg ttgtgcaact tctgtgatga tggcctggct gaggtagata
     7861 ataatgcagc agaaagagcg cttcgtgcag tctgtctcgg gaagaaaact ttttcgagga
     7921 tcactcattc cgtgtctgac tgcttcaggg tttaattttg cttctgctga ctgaatgatg
     7981 attgggcagc aagtgcaata tcccacataa atccagtcaa ctcacgagcc accgccgtaa
     8041 tggctacttg tgattttttc ccttttgctg tcagagttcg gtaacgagaa cacaatcgtt
     8101 gttgagcttt ccatgcaata tctttggaat attgtgttac agagtccggt cggtaatata
     8161 taagccatgc tccaactttt gcaggtgtac gataagacca ggcggcttcg taaagcagac
     8221 gtctcagttc gctgtttcca acttttgtta ttcctctggg acgaatactg tttccactgg
     8281 aatattcacc tggcaccag
//
LOCUS       DAJTPG010000148         8222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTPG010000148 DAJTPG010000000
VERSION     DAJTPG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="OO230_003651"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="OO230_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="OO230_003652"
     CDS             1127..1234
                     /locus_tag="OO230_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693259.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="OO230_003653"
     CDS             complement(1287..2045)
                     /locus_tag="OO230_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5693260.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="OO230_003654"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="OO230_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5693261.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="OO230_003655"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="OO230_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5693262.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="OO230_003656"
     CDS             3758..4618
                     /locus_tag="OO230_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5693263.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="OO230_003657"
     CDS             complement(4659..5195)
                     /locus_tag="OO230_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5693264.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="OO230_003658"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="OO230_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5693265.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="OO230_003659"
     CDS             6173..6763
                     /locus_tag="OO230_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5693266.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..8222
                     /gene="tcyP"
                     /locus_tag="OO230_003660"
                     /pseudo
     CDS             6896..8222
                     /gene="tcyP"
                     /locus_tag="OO230_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2135 a         1968 c         1971 g         2148 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccattatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     8041 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     8101 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     8161 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     8221 tg
//
LOCUS       DAJTPG010000149         7987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTPG010000149 DAJTPG010000000
VERSION     DAJTPG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..348
                     /locus_tag="OO230_003661"
     CDS             <1..348
                     /locus_tag="OO230_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5693267.1"
                     /translation="PITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLG
                     ISKRGDKNLRRLLVQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARI
                     AWAITTQKKEYQA"
     gene            630..911
                     /locus_tag="OO230_003662"
                     /pseudo
     CDS             630..911
                     /locus_tag="OO230_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(1061..1148)
                     /locus_tag="OO230_003663"
     tRNA            complement(1061..1148)
                     /locus_tag="OO230_003663"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1112..1114),aa:Ser,seq:gga)
     gene            1383..2321
                     /gene="ghrA"
                     /locus_tag="OO230_003664"
                     /pseudo
     CDS             1383..2321
                     /gene="ghrA"
                     /locus_tag="OO230_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            2376..3113
                     /gene="ycdX"
                     /locus_tag="OO230_003665"
     CDS             2376..3113
                     /gene="ycdX"
                     /locus_tag="OO230_003665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5693268.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3137..3691
                     /gene="ycdY"
                     /locus_tag="OO230_003666"
     CDS             3137..3691
                     /gene="ycdY"
                     /locus_tag="OO230_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5693269.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3793..4284
                     /locus_tag="OO230_003667"
     CDS             3793..4284
                     /locus_tag="OO230_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5693270.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4417..5123)
                     /locus_tag="OO230_003668"
                     /pseudo
     CDS             complement(4417..5123)
                     /locus_tag="OO230_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(5150..5566)
                     /gene="csgF"
                     /locus_tag="OO230_003669"
     CDS             complement(5150..5566)
                     /gene="csgF"
                     /locus_tag="OO230_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5693271.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5591..5980)
                     /gene="csgE"
                     /locus_tag="OO230_003670"
     CDS             complement(5591..5980)
                     /gene="csgE"
                     /locus_tag="OO230_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5693272.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(5985..6635)
                     /gene="csgD"
                     /locus_tag="OO230_003671"
     CDS             complement(5985..6635)
                     /gene="csgD"
                     /locus_tag="OO230_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5693273.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            7388..7843
                     /gene="csgB"
                     /locus_tag="OO230_003672"
     CDS             7388..7843
                     /gene="csgB"
                     /locus_tag="OO230_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5693274.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            7884..>7987
                     /locus_tag="OO230_003673"
     CDS             7884..>7987
                     /locus_tag="OO230_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5693275.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         2166 a         1736 c         1918 g         2167 t
ORIGIN      
        1 ccgattactg ccagcttact atcgtcgcaa cttggtgacg ggaaacagtt ctcatgcagc
       61 agggattttg cagcttccac aggacttgtt ccccggcagt atagcactgg aggaaaatcg
      121 actctgttgg gtatcagtaa gcgaggtgat aaaaatttac gccggttact ggttcagtgt
      181 gcccgctcat tcataatgcg acttgaacgc aagcatggaa aacttgctga gtgggttcgg
      241 gagcaactca acaagaagca ttcgaatgtg gttacctgcg ctctggcaaa taaactggct
      301 cgaattgcct gggcaatcac tactcagaaa aaagaatatc aggcctgata tagatgctga
      361 tataactcaa ttagtttatt ttaaacaata agatacaagt tacctttctg gttttgcgaa
      421 gactgattat tgatgacatg aacggcaaat cgacctgatt aaaaacctaa cagatgaaat
      481 ggctcactga agccgagggg atttttagga ttatcaggtg cggaactcat cggggcgcgg
      541 gcatccttgc ccacagagac gccggataga tttaagcaag ccaaccatta atcaaaaatc
      601 agtgttgcaa aaacgggggt gaccatagat ttcatcttat caccggacaa caggctgacc
      661 gactcgccga tctggaaaga atgttgcacc tgttcagcgg taaaccaatc cccgacaacc
      721 ggggaaacat cacccttcgt ctggatgatc acattcagtc tgttcagggt aaagagagct
      781 atgaagatga gatgttcagc atcagatact ttaagaaggg ctccgcgcat atcacgttca
      841 gaaaaccaga actggttgac agactgaatg atattatcgc aaaacactat cctgatatgc
      901 tggcagtata gctttgccag tatgctcaaa gatcggaaat tgtccgataa gatatgatcg
      961 cggggcatat aaaccggttt cagatggcat acccgatggc atactgtctg gaaggaaaaa
     1021 taaaaaagcg catgttaaac aataacatgc acttataatc tggcggtgag ggggggattc
     1081 gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac
     1141 acctcaccaa attgtcgttc ctgtcttgct ggaacgggcg ctaatttagg gaaatcatga
     1201 cctgaggtca acaaactttt tgaaaaaatc gcgcgtttat tcaaacattc aatcaatgtg
     1261 tggttttaat aagcgaaatc tgcttttttt gccaccgacc acggatttgt tatgctggtg
     1321 gcctttgtag atcataacga taagtgcgaa taaatttcgc acaacgcttt tcgggagtca
     1381 gtatggatat catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc
     1441 gcaaagctat tcctcaggca agagtcaggg catggaaaag cggagataat gactctgctg
     1501 attatgtttt agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg
     1561 tgttcgcact cggggccggt gttgattcta ttttgagcaa gctacaggca caccctgaaa
     1621 tgctgaaccc ttctgttcca ctttttcgcc tggaagatac cggtatgggc gagcaaatgc
     1681 aggaatatgc tgtcagtcag gtgctgcatt ggtttcgacg ttttgacgat tatcgcatcc
     1741 agcaaaatag ttcgcattgg caaccgctgc ctgaatatca tcgggaagat tttaccatcg
     1801 gcattttggg cgcaggcgta ctgggctgta aagttgctca gagtctgcaa acctagcgct
     1861 ttccgctgcg ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg
     1921 gacgggaaga actgtctgca tttctgagcc aatgtcgggt attgattaat ttgttaccga
     1981 atacccctga aaccgtcggc attattaatc aacaattact cgaaaaatta cctgatggcg
     2041 cgtatctcct caacctggcg cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc
     2101 tggatagcgg gaaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa cccttaccgc
     2161 ctgaaagtcc gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta
     2221 cccgtcccgc tgaagctgtg gagtacattt ctcgcactat tgcccagctc gaaaaagggg
     2281 agagggtctg cgggcaagtc gaccgcgcac gtggctacta ataaagcatc aggattcctg
     2341 ctatccttgg cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc
     2401 ataccgttgc cagcacacat gcatatagca cattgagtga ttacattgcc caggccaaac
     2461 aaaagggcat taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgcgccgc
     2521 atcactggca cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc
     2581 tgcgcggcat tgaagctaac attaaaaatg ttgatggtga aattgactgc agcggtaaaa
     2641 tgtttgactc gctggatctg attattgccg gttttcatga gccggttttt gcgccacatg
     2701 acaaagcgac caatacacaa gcgatgatct ccactatcgc cagcggcaat gtgcatataa
     2761 taagccatcc cggaaatccg aaatatgaaa tagatgttaa agccgttgct gaagcagccg
     2821 cgaaacatca ggtggcgctg gaaatcaata attcctcatt tttacactca cgtaagggca
     2881 gtgaagacaa ctgtcgtgcg gtagctgcag cggtacgtga tgctggaggt tgggtggcat
     2941 taggctcgga ttctcacacc gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc
     3001 tcgacgcggt agattttccg ctagagcgca ttttgaatgt ttctccgcgc cgcttactga
     3061 acttccttga atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag
     3121 taatggaaat atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta
     3181 ttaccgccaa ccgcaagatc ctttactggt gccgctgttt actctgattc gtgaggggaa
     3241 actggctgcg aactggccac tggagcagga tgagttactg acgcgtttac agaaaagctg
     3301 tgatatgacg caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgccgt
     3361 gccgccatat cgtagcgcat gggttgagga cgcaacggaa gcggaagtgc gcgcttttct
     3421 ttccgaacga gggatgctac tggcggatac gccagccgat cacatcggca cattgctgct
     3481 tgcggcttcc tggctggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact
     3541 gttcagtgag tatctgttac cctggtgtgg tgcgttcctc ggcaaagtgg aggcccatgc
     3601 aaccacgcct ttctggcgca ccatggcacc gctaacccgt gatgccatta gtgcaatgtg
     3661 ggacgagctg gaagaagatt ctgaagagta aatgtgatca tcgtcactca taactgcaac
     3721 agataatttg ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct
     3781 atcatgcgcg gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt
     3841 atctggggat gggtggctgt ttccctgggc ttactaagct gggcgggctt cctgggctgt
     3901 acggcctact ttgcttgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc
     3961 ctgcttagtg gcgtggtgtg ggcgatggtc attatttacg gtagtgcact ggcaccacat
     4021 ctggaaattc tcggttatgt cataaccggt attgtcgcgt ttctgatgtg tattcaggcc
     4081 aaacagctgc tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc
     4141 gggcagggtg actggaaact ggtgttacct tcgctggcgc ttgggctggt atttggttac
     4201 gcaatgaaaa acagcggcct gtggctggcg gcgcgtagtg caaagacggc tcaccgtgag
     4261 caggaaatca aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca gtaaatgccg
     4321 gattattaat tccggcattt ttatctgtca ggattccggt ggaaccgaca tatggcggta
     4381 tttcaccaga atgtcattct gccgttctgc tttattttac aaatcccaca gaccacggtc
     4441 gataccatca ttaatcagga aaatgacccc tgtttcgata gccgacatca gacacagcat
     4501 aacgggttcg tttgaggtgt aacccacttc cccttccagc aagcgctggt agtcaataaa
     4561 gcggaaaacc ccggcctgaa cttcatagga aagtatcgtc ttactggtgt tcaccgagga
     4621 gaggatctca ccggtactca cattgacgac acgcaggttc acggcaatct gatcgagctg
     4681 gtattgcgtg tctgcaccga tcccaaaata tcttgcccca acaccgcccg atttaacgtt
     4741 gctttcataa ccgataatcg aaccttcaac cataatattt gccgccgtta aagattgcag
     4801 cgggattcgg ttattaatgg caaccgtgcc gttttcttgt gccgcacgaa taaccttgcg
     4861 ctcgttaagc aggttttgta agccctggcg ctttgcggaa cagcagtgga gaagttactt
     4921 gccgggtagg gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc
     4981 ttacccgtcg gcgctggcag atgggtcaaa tctttgtagc tctgagcacg aggcattaat
     5041 gtcggtctgg cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca
     5101 accaaaagaa ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg
     5161 ttgagttatt ttgtaaaccc gaaacctgga tggtcgaggt ttgtccggtt ttacgatctg
     5221 tcacgttcaa ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca
     5281 ccatgcggcc cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact
     5341 ggatggcctg agtaaagtta tctaacgctg agggtgtttc aataccaaag tcatcgttat
     5401 agctcggatc tttataagag ttttgggcct gagcgctatt taataaaaaa gcgccattat
     5461 ttgggttacc accaaagttt ggattacgga actggaaagt catggttcca gcccaactta
     5521 atggcgaaat aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac
     5581 aattttttat ttagaattca tcatgcgcca aatcgtccgt acttaataac gcctgattta
     5641 tctggcgacg atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttctcgg
     5701 agtctctttt caacgggaat aaaaaagtct ggaaaataac atcctgattg accgttattg
     5761 tgatccagct tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt
     5821 cactttccca tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat
     5881 ggtcagttaa caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca
     5941 gaaattctgc cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta
     6001 tcgtttgccc aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta
     6061 taaagatgcg ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta
     6121 gacgcgccga tacgcagctt attcaggatc tctttttccc gatgagtaag gagggctgat
     6181 tccgtgctgt tataacgata gttaccagaa tgtgtaatca ggtaactggc aagcttttgc
     6241 gtaaagtagc attcgccacg caggacgcct tgcaacccat tgacaacacg ttcttgatcc
     6301 tccatggcat aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa
     6361 tcttcaggcg tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc
     6421 tgccaataat ggataagctt tttatccgct tccatcatat ccagaagaat aatagagcct
     6481 gaagagatat cgtccagaga acgttgaata ttatgtaatt ttcctgtaat tgctagcgat
     6541 tgtttaaggt gctgcaagag agctgtcgcc tgcaaagaag gtttagtgat caacaataat
     6601 gtatgaccat gaatactatg gacttcatta aacatgatga aaccccactt ttttattgat
     6661 cgcacacctg acagctgcct ctaaaataga agcaccagaa gtactgacag atgttgcact
     6721 gctgtgtgta gtaataaatc agccctaaat gggtaaaata taaaactaat ggattacatc
     6781 tgatttcaat ctagccatta caaatcttaa atcaagtgtt aaacatgtaa caaaatgtaa
     6841 ctcattatat taaaatgtta acattaaggt tttattaagt ttagaaatga tagaaaagtt
     6901 gtacatttgg tttttattgc acaattttaa aaaatcatac aaatggtgct aacttactaa
     6961 taatacatat aaaaaatatt tcggtgtagt cctttcgtca tgtaaaacgt ttttgttttt
     7021 tctccacacc tccgtggaca attttttact gcaaaaagac gaggtttgtc acggcttgtg
     7081 cgcaagacat atcgcagcaa tcagcgacgg gcaagaagaa tgactgtctg gtgatttttg
     7141 atagcggaaa acggagattt aagagaaaac aaaatatttt ttgcgtagat aacagcgtat
     7201 ttacgcgggt tttaatactt tggtatgaac aaaaaaagaa aaatacaacg cgcgggtgag
     7261 ttattaaaaa tatttccgca gacatacttt ccatcgtaac gcagcgttaa caaaatacag
     7321 gttgcgttaa caaccaagtt gaaatgattt aatttcttaa atgtacgacc aggtccaggg
     7381 tgacaacatg aaaaacaaat tgttatttat gatgttaaca atactgggtg cgcctgggat
     7441 tgcagccgca gcaggttatg atttagctaa ttcagaatat aacttcgcgg taaatgaatt
     7501 gagtaagtct tcatttaatc aggcagccat aattggtcag gctgggacta ataatagtgc
     7561 tcagttacgg cagggaggct caaaactttt ggcggttgtt gcgcaagaag gtagtagcaa
     7621 tcgggcaaag attgaccaga caggagatta taaccttgca tatattgatc aggcgggcag
     7681 tgccaacgat gccagtattt cgcaaggtgc ttatggtaat actgcgatga ttatccagaa
     7741 aggttctggt aataaagcaa atattacaca gtatggtact caaaaaacgg cagttgtagt
     7801 gcagagacag tcgcaaatgg caattcgcgt gacacaacgt taattaccat tcgactttta
     7861 aatcaatccg atgggggttt tacatgaaac ttttaaaagt agcagcaatt gcagcaatcg
     7921 tattctccgg tagcgctctg gcaggtgttg ttcctcagta cggcggcggc ggtaaccacg
     7981 gtggtgg
//
LOCUS       DAJTPG010000150         7926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTPG010000150 DAJTPG010000000
VERSION     DAJTPG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..517
                     /locus_tag="OO230_003674"
     CDS             <1..517
                     /locus_tag="OO230_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693276.1"
                     /translation="ELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGT
                     VKTALENLGLGEAAKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGA
                     PNVSSTYGMRFPMTFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGS
                     SADAYYIAMGY"
     gene            489..923
                     /locus_tag="OO230_003675"
     CDS             489..923
                     /locus_tag="OO230_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5693277.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1696..2745)
                     /locus_tag="OO230_003676"
     CDS             complement(1696..2745)
                     /locus_tag="OO230_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5693278.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINAINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3196..4386
                     /locus_tag="OO230_003677"
     CDS             3196..4386
                     /locus_tag="OO230_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5693279.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4776..6236)
                     /gene="gtrII"
                     /locus_tag="OO230_003678"
     CDS             complement(4776..6236)
                     /gene="gtrII"
                     /locus_tag="OO230_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5693280.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6233..7162)
                     /locus_tag="OO230_003679"
     CDS             complement(6233..7162)
                     /locus_tag="OO230_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5693281.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7159..7521)
                     /locus_tag="OO230_003680"
     CDS             complement(7159..7521)
                     /locus_tag="OO230_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5693282.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2453 a         1499 c         1597 g         2377 t
ORIGIN      
        1 ggagctttgc agcgtggtgg aggcatccgg tgccagcctg gagaaggggc ggcacgatca
       61 gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag aatccgtttg gcgatatcaa
      121 atcggatggc acggtgaaaa cggctctcga aaaccttggt ttgggagaag cagctaaaag
      181 gaatgtaggt acaggggcga atcagatacc tgatatgagc ctgttcgcgt caattaatac
      241 cgtaacggct gctgcgcaaa aatttccgtc tggattaatt ttacagtgcg gtcagttgaa
      301 tggtgccccg aatgtatctt caacatacgg gatgaggttc ccgatgacgt tctcaagagt
      361 aattgctgtc gtagttacat tgaacgttac tggcgcggct gggcagccga ctgtatcggc
      421 gacaagtgtc cagaacactg gatttaatat tactgtgtcg cccggttcag gatacggttc
      481 atctgctgat gcgtattaca ttgcaatggg atattaacaa aatgtcatat ttttattctg
      541 catcgacaaa cggattttat tcgactgaat ttcacggcac caatattcct gatgatgcag
      601 tggaaatctc ggaatcagag tggaaaacac tgattaatgc acagagtgta acaaaaatga
      661 ttacctgtgg tgagaacggt catcctgtca ttgttgaccg tccttctcca acaccagaac
      721 aattagcctt aataaatgat gaaaagaaat ctgcactgat agcagaggca acgaatgtaa
      781 tagctccgct tcaggatgcg gttgatttag gtatggcaac agatgatgaa acgaaactgt
      841 tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt taatgtagta aaacccgagt
      901 ggcctatgca tccaaataaa tagacttgtt aatctaggat aattgttgga ataatactcg
      961 tttttcagta ggttcaggta gcctgggtat aatgctcact tctacaatga tttcaactta
     1021 ttttatttaa ttgatattac gaaacttaac tcatacataa acaatatgac tcaatactgt
     1081 cctaaatagc tgcgcagaaa agtgctccac tttcgctcag cccttgctgg gcgaagcatc
     1141 aggaaggcgt tctcagctag gttgtgttat atctggcttg agttttgttg cccatacgca
     1201 acacattacc catctgtaaa cgacaagtgt ggcagaatta ggtgcagcta tttagactat
     1261 atcgacgcag taacgataat agatatcgaa actgtactag atattagcta cttatggtta
     1321 ccggggggag tcttttgagc actggtgtta tcaattggct tcaaataatt cgccaataaa
     1381 ttatattgcg atagctcatc accttccagg ttgtttatag ccagtttatc agcgtcacca
     1441 cctactgttt atgcttttat ttctttgatt aataagtata tgatatttta attcatctgg
     1501 tgtatttctt tctcatgtgt tggtcgactg aatatctgtt cagtgaaggg gtaattttat
     1561 gtttgcactt cgaacattcc cttcagttgc taggaaagaa aaccgtaaca tttgcgcatt
     1621 aattttaaca gtataatctg agcataaata tgcgctatat atctataaaa aggcgcaatt
     1681 aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg actgtcctcg atagccaatg
     1741 gccaatttta tctgatacat atacacaaat acaggtatat atgattgcgc agataggatt
     1801 tggaatagtc acgctgaagc ctataattgc taaagccata gggataaata atttggtgat
     1861 atactccatt ccacatatgt taagagtatt tcttccagct cttacaataa agtcatttcc
     1921 acaaattatt tttgctatga aaatattaaa gattatcaag cctaccgtgg tgataatagt
     1981 attaatagct tgaatataac tacttgatat tatgtctgca ttgaatattg agataatctt
     2041 aaaaagtaaa ggtttctggt taagtagttg atacgcactc attatagcgg taattgagaa
     2101 gacaatgaaa ccaattttag attttccaaa aaacctgtca cgtgttaatt ctaaaaacat
     2161 acatctaccg agaggcaata accaccagta tgccatagcg gagtctatgt tccaaaacca
     2221 ttgaggatct agtaatgggt tatgattgag cactgtttgt gacaacatga acgataacag
     2281 tgatatgatt aaaatcacta tattgttctt gactatttct ctaaagattg catcaattgc
     2341 tattataaca aaaaggcagt ttataaacca tattgttcct acgaattggt tatttctaac
     2401 accatatatt atatcataaa tgtggttgta tatataatca atcgtctcac ctgagttaat
     2461 tgcgtttatt attaatatag aaaatgcaaa agtgaaatat ggaatcatta atctgtaaaa
     2521 ctttgacttg atataatcga aaacagataa gtcatttttt ttgatagtaa aaaaaccagc
     2581 agcgaaaaag aatagtggga cgtgataact aaaaacaaaa ggatacaatt tgccagcccc
     2641 caacccaagg tgacctaaat atatagcaaa aataccgaga aattttaaag catcaaccca
     2701 atcatgtctt atttttctgt tgctgtcgat tttgttatta tacatttaaa gtaggctctc
     2761 cagttttgtg tgatttttaa acactcattg caaatatgaa tgcctgaaag tttatgatta
     2821 ttaatcaacg agttcaatat taatttatta tttttcaaag tcgcactata taggcattta
     2881 cttccgcagt accaccgata gtatcgatat caaatcagcc tgcggccgcg ataactttcg
     2941 taacattctg ccagcggctt tgttgaataa atcaggttca agataatcgt ctctgcatca
     3001 ggttagagtt tcaggcaata catcctcttt ctggcatacc tgccactctc gcaccatggt
     3061 catagccttt acgatctgca tactcaccag accagagccc gctccctttt aggctgagga
     3121 atccccagaa aagaagacag gcatagagtt ttgtgatcta ctgattgcgc taacaaattc
     3181 aatgagatca cctctatgcc tgcccgtaaa gtatgccaga cttttttccg taatgcttta
     3241 gcttccacgc atcagtatcg acaaaatgcc attattgatt ctgctgctgc tttagtcggt
     3301 ggcgcatctc tttctcttac cagcattggc cggcatttac ccggtcctgc tcgcgtgaaa
     3361 gataaaataa aacgcgttga ctgtctgttg ggtaataaac gacttcataa tgatatccct
     3421 ctgatattca aaaatattac atccatgatg acaaataaac tgtcgtggtg tgttatcgct
     3481 gttgactgga gcggttaccc ttttcaggaa tatcatgtcc ttcgtgccag cctcctctgc
     3541 gacggtcgct ctatccccct gatgagtcag gtcttcccgt caaaaaaaaa taacgaggcg
     3601 gtggaaatcg cttttctgga tgcgctttcc ggggccattt ctccccggac tcgtgttgtt
     3661 attgttactg atgctggttt tcaaagtgca tggttccgtc atatcaaatc acagggatgg
     3721 gattttattg gtcgtatcag gggggtagtg aagttcaggc tggatagtga caaagataag
     3781 tggctggaca taaaaatgtg cagggggagt tcagaggcaa aatacctggg gacgggaacc
     3841 ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt atctttataa acattcacct
     3901 aaaggcagaa aaagcaggcg tgccagaggg aggccgggct taccaacaac ggaaaaagaa
     3961 caaaaagccg caggcagaga accatggtta atattcagca atacagctga gtttaatgcc
     4021 aaaaaaatca tgaaactgta cagtcgaaga atgcagatag agcaaaactt tcgggatgaa
     4081 aaaagtgaac gctttggttt tggccttcgg gcgagcagaa gtcggagggg agagcgtttt
     4141 ctggtattaa gtctactggt tacactggcc agtatcgtac tctggctact tggttatcat
     4201 tttgaaaata aaggatttca tttaaagtat caggctaata gtctcaaaaa acgaagagta
     4261 ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta accctgaact tctacgacga
     4321 gcacaacctg aaaagatatt gtgccggttg gccagcactt accgaagtat ggtgctggct
     4381 tactgatgaa aattttctgg ggatccctca gcctttcggg gaggaatgag ggctctgata
     4441 ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat ttggcctgag tgacccatgc
     4501 ctcccaaaag cagagtcgct agtgttatgt aattaggcgg ttaaacttca attctcagtg
     4561 ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt tttaattgac taatattaga
     4621 aacggttagt gtgattgtat atagatattt gagtgtaagg tatttaaagg ggcattcagc
     4681 ccctccgtct atttgtgatt gcatccattg atgtgtgtat tattttgagc ttgaaaaagg
     4741 gaggcgctct ttaataataa ataaatctat caaagctaaa tattaaatgg aagccaccct
     4801 tttttctctg atagttttat tatcaccaaa ccatttattt cagctatcga atctggattc
     4861 ggccaggaag gtaaactctt cacaattgga taaatcttct ctatttgttc tttatttgct
     4921 ggtttgcaat tacagattcc atagtaattc ataaatgcaa ccatcctaaa atagttgcca
     4981 ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc tcatggagag catatttcct
     5041 gagtcgtttg aaccatatat atatatatat tttccgttaa gggaaatgcc ttttgtttga
     5101 gatgtgtatg atatttcttt agctaaagag atatctttct gtcttgcgag agtatcattc
     5161 aaaaatagat ttttggatgt tgaaacgcca ttaaatataa taaataaaaa aaacatgcag
     5221 tttaggtatt ttatagagcg aaaattgctt aagcaagaaa acaaaattac tgctactata
     5281 ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa caacaacgat aaatataacc
     5341 ggaagtatat aaatgaaaat gattgatgaa atcaaataaa ttgatcttgt ttttaattta
     5401 tatgttaggg taaacatcag aattaataaa ggaatcagta accacttgta taggtttaaa
     5461 ccagtgggag gattgttata gaagttatat atattatcta tggctgattt aacaagccat
     5521 ttaatatcgc ttgcattttg tatataattt gagatgtact cgttcgattc aagcgaaaaa
     5581 taatgcttga tagctttggt taatagcagg taaattaagg tagataaagc tatgagggat
     5641 agagataaac atgaactata aaaagattca cgaatatttt tctcatttcg tattatcgaa
     5701 tttatctgca acccaatgac gaatgcaata atatatgtta cgaatgtttg gtagattgcc
     5761 attgaaagaa tggacagtac tataccagaa aatatcacaa tcctattttt ttgcgagtga
     5821 aaaataattg ctgatatcgc tgccagtaga aaagcaattc ccacagtatc agcttggtta
     5881 agaaactcta attgatagga gatctgaggg aaggtaataa atactaacat acctatcaat
     5941 aattcataag attctagctt tagcgatctg cagattataa atgctgaaat gatgataaaa
     6001 gataaggcta ttaatggtgt tatataaagt gaaaaaggct cagggagaaa gtaatgtcgc
     6061 aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat aaaaattatt tgtaaactcg
     6121 ccatcaatat taagtgttcc atcgtacaac cacccccaat aacaataaat tgatattgca
     6181 aaacatgata tgaaggcaag gagatttgca tttttaaata aattaatttt aatcatgggt
     6241 tacctcgatg agattttttt atgatgtatt tgggtctatt ttttacttca atatagattc
     6301 tacctatata ttctccgaga acaccaatcc cgatcagttg cactccaccc aagaaaagta
     6361 ttgatacaag cagggaggga tacccgcgta ctgggttacc aaagacaagg gtgtctataa
     6421 tcatccatgc accatataaa aatgaaatgc ttgcaacaaa caagcctata taagtccata
     6481 cgcgaagagg aaaggttgaa aaacttgtga taccttccag tgccagattc cataatttcc
     6541 agccattaaa ttttgagatg ccagcaacac gctctgcgcg tacatattca acgacatccg
     6601 tcggaccacc cacccagctc agtatgcctt tcatgaaaag attgcgctca ggcaacagtt
     6661 taatgttctc cacaacctca cgagacatga gtcgaaaatc tccgacattt tcctcgatct
     6721 ttggggtgct tattttgttg tgtaatttat agaaccactc agctgtctta cgtttcagtc
     6781 gtccatcagt tgagcggtct gaacgtttag caagcaccat gtcagcacct gcctgccact
     6841 tttcaataag atgagggata acctcaattg ggtcttgcag gtcgacatca ataggaatta
     6901 cagcatcgcc gcttgcatgg tctaaccctg caaataaggc tggttcttta ccaaagttgc
     6961 gtgtaaatga cagcggaaca actagcgggt ctgaaacagc cagcgcgtta ataattgact
     7021 ctgtggcatc tttacttccg tcatttatga atacaatttc tacttcatat ggcttcaact
     7081 cttggaattc acgtaccgtt ttatagaaaa caggtatcgc ttcttcttca ttgaagacag
     7141 gaacgacaag agatattttc atttcgcatc cctaaagaca atgaactttg aatagacgaa
     7201 accgcacacc agactgatgg cggagaaggt gataagagtg actattggag gaagtgagca
     7261 tttatcagct gcccaaccaa caatcacact caatattccc ataaatccca cgtataacat
     7321 gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt gcattcgcga agaagctaaa
     7381 gctcacagcc actacgaaac ctgtgaagtt tgccagagcc tgactggtat gtgcggcata
     7441 gatacataca ccaaaaacca cccagtgtat aagtgtgttc agcacaccta tagatgtgta
     7501 ctttacaaat aactttaaca tttatttaat caatgagctc tgaaaggcat gaagtctatc
     7561 atccaagtct caattgatcg atacttgcta tgtctgatga gacaaaactg agacacataa
     7621 ggcctcacaa tggcttgcaa ggctttacat gttttgatgt ggtgggacgt gtgagcgcag
     7681 tgttgatggg ataatccttt gaattacaag cggattctta taattcgtaa tgcgaaggtc
     7741 gtaggttcga ctcctattat cggcaccatt taaatcaata agttacctcg catttaagta
     7801 aactacgttc tcctcttgtg ccgtatttgt gccattgcga cttataatcg catcgatttt
     7861 gctcgcgtgc tcggtaagat gcccggctga aaggtgggcg tatctttgaa ccatttcgag
     7921 agtttc
//
LOCUS       DAJTPG010000151         7868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTPG010000151 DAJTPG010000000
VERSION     DAJTPG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..1185
                     /gene="yjeM"
                     /locus_tag="OO230_003681"
                     /pseudo
     CDS             1..1185
                     /gene="yjeM"
                     /locus_tag="OO230_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1336..4647)
                     /gene="mscM"
                     /locus_tag="OO230_003682"
     CDS             complement(1336..4647)
                     /gene="mscM"
                     /locus_tag="OO230_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5693283.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4669..5637)
                     /gene="psd"
                     /locus_tag="OO230_003683"
     CDS             complement(4669..5637)
                     /gene="psd"
                     /locus_tag="OO230_003683"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5693284.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5734..6786)
                     /gene="rsgA"
                     /locus_tag="OO230_003684"
     CDS             complement(5734..6786)
                     /gene="rsgA"
                     /locus_tag="OO230_003684"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5693285.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6881..7426
                     /gene="orn"
                     /locus_tag="OO230_003685"
     CDS             6881..7426
                     /gene="orn"
                     /locus_tag="OO230_003685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5693286.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7637..7712
                     /locus_tag="OO230_003686"
     tRNA            7637..7712
                     /locus_tag="OO230_003686"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7670..7672,aa:Gly,seq:gcc)
BASE COUNT         1796 a         2045 c         2134 g         1893 t
ORIGIN      
        1 cgcgaaggtt tgggtaccgt tctcaacatt cctctatggt agtgacatga cccagcactg
       61 gcgtattgcc ggactggagc ctacgcaggt ggttggtctg ctggcagtag catggatgat
      121 tctggtcaca gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg
      181 cggtattgca gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt
      241 attaaatggt gggcatttcg cgcaggatat taatttcctt gcatcaccga acccgggtta
      301 tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat
      361 tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg
      421 tattgttttt gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg
      481 cgtcagcaca aactggcagc aggtattaag taatggttcc gttaaccttg gcaatattac
      541 ctatgtgctg atgaagagcc tcggggtgat gctgggtaac gcactgcatt tgtcacctga
      601 agcgtcattg tcgctgggcg tatggtttgc gcgtattacc ggactttcga tgttcctcgc
      661 ttataccggt gcgtttgcta ttcaccgctg aaagccatca tccaggggac gccgaaagcg
      721 ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccgtctat cgccatgtgg
      781 atgcagtgcg ggttggttac tgtcttcatt ctgctggttt cgtttggtgg cggtaccgca
      841 tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct tccttacctg
      901 ttcctcgcgc tggctttccc gttttttaaa gcacgtcagg atctcgacag accatttgtg
      961 attttcaaaa cgcgtatgtc ggcaatgatt gcgacggtgg ttgtcgttct ggtggtgaca
     1021 tttgcgaacg tcttcaccat tattcagcct gtggttgaag ccggagactg ggacagcaca
     1081 ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat ttaccagaac
     1141 tattgcagtc gcatggcgaa taaacctgag ttagctctcg actgatcata tgaaatcctg
     1201 caactcatcg tctctccggc gatgagttgt caggaagtaa tgcgtatgga tgatactctc
     1261 cagggccggt gataactgaa gatgatgtta agctgctaaa tgagccacgt tgacatcatt
     1321 tggcagcttc atgcattaca aacttcccgc ctgacgacct ttgcccgcag acgtcagcgt
     1381 tctccccgtt tgtttaccgt tgaggctttc cagacgcatc tggaagggcg ggaatggcat
     1441 atcgataccg tgggcgtgga agcctgccag aatcagctgg tggatctcat ggcgtagcgg
     1501 catacggtga cccatctcag cggcgtaaat acgcagctcg aaaatctgaa tcccctgttg
     1561 cagatccacc aggaagactt ccggtgccgg gttgtcgatc accagcgagc agcgacgcgc
     1621 tgcggtgagc aggatttccg tcacttcttc actgttggca tcggcagggg ccggaatcgt
     1681 caacactacg cgcgtgaccg agtcagagag cgaccagttg ataaactgct cggtaataaa
     1741 cgccttgttc ggcacgatta tctctttacg gtcccagtcg ctgatggtgg tggcgcgggt
     1801 gttaattttt gtcacgctac cagtaagatc gcggatcgtt accgtatcgc caatgcgaat
     1861 cggtttttcg aacaggatga tcaggccaga gataaagttg gcgaaaattt cctgcaaacc
     1921 aaaaccgaga ccaacaccga gcgcggcaac cagccactgc aatttcgacc actcaatacc
     1981 aatcattgag aagccgacca gcccgccaat cagcatcagc agatattttg tgatggtggt
     2041 gatggcgtaa cccgtacccg gcgttaaatc caggtgctgc aaaatcgcca gttccagcag
     2101 cgcgggcagg ttgcgcacca gctgcgtggt gatgataaat accagaatgg caatcagcac
     2161 cgcaccgagg gtaattggct ccagactttc tacgccctgt accgtggagg tgacatccca
     2221 cagcgaaata ttttcgagga agccgaaagc ggaatggatt tctgaccaca gcacaatgac
     2281 cgaaagcagg gcgatcaaca tcaaaattga gcgcaccagc cgcaaggatt gcgcactgat
     2341 ggcatcgaga tcgacttcgc tttcatcgac ttcaattgct ccttccgggc tactgtgatg
     2401 atgcgcttcc tcttcgccac gcgcacgttg cgctaacatc tctgcccggc gatgcttcgc
     2461 ccggtcaaac gccagcctgc gacgctggat cagcatccag cggcggataa cgtgataaac
     2521 caccagtagc aggaaccaga tggcaaccga ggtttcaagc ctcgctaaca gtgcctgcgc
     2581 cgttgccaga taacccaccg ccgacgccag aatggcaacc agtggtgcgc caatcatcat
     2641 gttccacagc atatggttgg taatgttgtc gccgctgcct tctttgttga gatacaacgg
     2701 aataccggct tttttcaggc ttagggtgac caccgccagc gcaccgcaaa tgaggataaa
     2761 gcaaagccgt cccagcgaac cggaaaattc gcggtcgtcg aggttatcga acatcatcag
     2821 cgccataatc agcccgatgc tcatcaggta gtagcgcatc ccacgggaaa cacgttcgcg
     2881 cggccagcca aaatgagcga taaacaagcc gttcgggcgg gcaaaggtgg cgcaaatcat
     2941 cactacccac agcagcggca cggtggccgt tacaccatcg ccaatcgcga ccgccagcgg
     3001 ataaggccac gcctcgcgca agccgtaacc cagcgtcatc cacagcaccg gtaacggtga
     3061 cgcgacgaga atcgaccaga aaagagtgcg caacgtcagc cagaagtgat cctgagtcac
     3121 tttgccgact ttcgccgccg aacgttcaag aaaacgggtg aaatagcggc gcgagtaaat
     3181 actgcaaccg accagaatca acgcgccaaa cagcggcaaa attgtctctt tgctggtcag
     3241 catcatcaca ctggctttgc ccaactgact gaaggtgtcc agcgaaatga gacgacgcag
     3301 atcctgggcg atttccagcg gccaggcgat ggtcatcggg cgcacgtcag aggcccagaa
     3361 cagatagcgg tgcgttgctt cgttcacctc tttcagcgca tcctccagtt gcctgttgga
     3421 gactttcagc ttggtcagtt ccaatagtag cgtgtcgcca ccctgcaaca atgagttcag
     3481 caactcacgc tgagtgcgca gttgtgcttc cagaatacgg ttttgctcgg cggtcagcgg
     3541 ctgaccgtcg gcctgatgga tttgccgtag cagcggctgt ttattaagta aatcctcata
     3601 acgtaaccgt tgcacacgca actgcgccat ttcggtatca agctgttgtg gttttggcat
     3661 ttctggtagc cgtgccacct gcgcccgcag cgcttcgccg agcaggttgg acgatcccag
     3721 ccattgcgac tgttcacgca gcgtattcaa cgcctgccgg acctgtaacg tctggctggc
     3781 agcctgacgc tgttgcgagg caacgagctc catccgctgc gcctgttgat tcagcgctgc
     3841 cgatagttcg cggttaattt tgaattgcgc gacgatatct ttcggcaaat cggcactgtt
     3901 ttctgccagt aattcggtac tttccagcgc ccgctccgcc tcaagctgac gttggctatt
     3961 caactgatta cgtaaggcct gcaaatacgc atccagttgc tggctctctt tttccgccag
     4021 ctctgagcgt aagcgcgcta attcctggcg gttattggca gacagctgcg ccagctccag
     4081 ttcatcaacg agcgccttaa gacgtgcaga gtcagactgc aacgcgaaat tttgtgcctg
     4141 attgagcgga gtattgccgg taagcgttcc caggcggcgc tcgatctcat ttaactgacg
     4201 gcgggcgtcg gtttgctgtt gcggcagttg attcagcgaa tcggcaatct cgcgggcgcg
     4261 ctcctgctct tgctgggcct gacggctttt atccagcaac tggctgctga cctggagaat
     4321 ttcctgattc agcgcgtcgg tagacattcc cggcgacacg ctgcgcggct cgtcacgaat
     4381 gttgtttaat tgtgcgcgca gagtagcgga gagtttcgga taattatcga taacttgctg
     4441 atattgtttg atgcgctcaa gggaaccttt tcgttcctca agcgcattta aggcagactg
     4501 gagcgcctct acgacttccg gctgtgcggg tttcgccgct tttgcctgct ccagttcctg
     4561 agtgatttgt ttgctatcgg gggccgtcgc ggcgtacgcc ccccaactga ggcaccaggc
     4621 catcagaaaa gtgataatca ggcgcacgtc agcgtttcct ttgatggatt agacctggtc
     4681 ttttttatcg tcaaccaatg ggctggcgtc gtgttctgct tcgatctctt cagcaggaag
     4741 tggggcgggt tcagcgtctg gcgtaacaaa ggtttcggta gatactgcca gcggctggcc
     4801 aattttcgtg acagacaggc tttccagttg ctcaaccaga ttcactttac ccggtgcaaa
     4861 caggttgata acggtggaac cgagtttaaa gcgacccatt tcctggcctt tcagcagtgc
     4921 cacagaaccg tcgttttccc cggcaggcca ggtccagcgc ttgatgatac cttcgcgcgg
     4981 cggcgtaatg gtgcccgccc agaccgtctc aatgctgcca acaatcgtcg ctccgaccag
     5041 aatctgcgcc attgggccaa attcggtatc gaaaaggcaa atcacgcgtt cgttacgggc
     5101 aaacagattt ggcacgttct gagcggtgag atggttaacg gaaaagagat cgcccggcac
     5161 gtagatcatc tcacgcagaa taccgttgca cggcatgtgt acgcgatggt agtcacgcgg
     5221 ggagaggtaa gtggtcacaa acgtaccgtt gcggaacagg tccgccatca gatagttgcc
     5281 tgccagcagg gcttcgaggc tgtagttgtg gcctttggct tgcaggattt tatcttcttc
     5341 gattttaccc aactggctga taacgccatc ggcaggcatg accagtacat tcggatcggt
     5401 atcgattggg cgtacttcgt cacgcagcgg acggacaaag aattcgttaa aggtgcggta
     5461 gctggcggtg tccggctttt gcgcctcttt catgtcgacc ttgtagtatt taacgaacag
     5521 atcgataacc agttttgtca gccatcctgc ccgcttgctt gcgccccaac ccgccaggcg
     5581 agtaagccat agtttcggca gaatgtactg tagcgaaagt ttaaatgaat ttaacaaggt
     5641 agcctccagg ccattgtttt gtcgttcctg atccggccaa catgccggat cctgaaaaaa
     5701 aggggacgat tctaacgacg gttagcttaa ttgtcagtca tccgtatcag aaaagttttt
     5761 acgcgttttt acctgcgcca tgctttccag aatacggtga tagttttcga aacgggtttc
     5821 cgcgattttc ccttcctcaa ccgcttcccg gatagcgcag cccggatcgg tatcgtgttt
     5881 gcaatcgcga tatttacaca gacctaaata gtcatggaat tcgacaaagc cctgagtgat
     5941 ttgttccggc tccaggtgcc agaggccgaa ctcacgcaca ccaggggaat caatcacatc
     6001 accgccgtgc gggaagtgat acagccgagc ggcggtcgtg gtgtgctgac cgagccccga
     6061 gttgtcagag acatcgttgg tcaggatctc tttttgcagc cccagcaacg cgttcagcag
     6121 gctggattta ccgacgccag actgcccggc aaaaatgctg atgcgcccgg tcaacgcctc
     6181 ttccagcggt ttcagcccat cctgagtatg gctggaaacc atcaatacgc gataaccgat
     6241 gttgcggtag atatccatct gctcgttgac gaacgccatg ccttcgtcgt ccagcaggtc
     6301 tatcttgttg agcacgataa tcggctcaat ctgcaaggtt tcgcaggcca ccaggtaacg
     6361 gtcgataata ttgagcgaca gctccggcaa aatggcggag acaatgacaa tctggtcgat
     6421 gttggcggct atgggtttca cgccgtcgta gaaatccgga cgcgttaaca ccgaggtgcg
     6481 ctcatgcacc gcttccacga tccctttgac atttacgcct tccgccgccg gtttacccgg
     6541 acgccagact acgcggtcgc cggttaccag cgaacggatg gtacggcgaa tattgcagcg
     6601 gtgaacgtcg ccatcggcgg attccacatc agcgtgcata ccaaagcggc tgatgacgat
     6661 accttcatca ggctcgccaa acagattgtc gtcgtagtcg ggcttctcct tagacgtttt
     6721 aagacgacgc tggtgattgg cgttcacgcg gcgctgctgg cctttggaga gtttattttt
     6781 actcaatcgt actgactcct ggtcgcccct gatgggcaaa acatctatga tacacgcaat
     6841 tgtggatcaa tatagtcact gtgaatgggt ggaaaatagc atgagtgcca atgaaaacaa
     6901 cctgatttgg atcgatcttg agatgaccgg tctggatccc gagcgcgatc gcattattga
     6961 gattgccacg ctggtgaccg atgccaatct gaatattctg gcagaagggc cgaccattgc
     7021 agtacatcag tccgatgaac agctggcgct gatggatgac tggaacgtgc gcacccatac
     7081 cgccagcggg ctggtagagc gcgtgaaagc gagcacgatg ggcgatcggg aagctgaact
     7141 ggcaacgctc gaattcttaa aacagtgggt gcctgcggga aaatcgccga tttgcggtaa
     7201 cagcatcgga caggaccgtc gtttcctgtt taaatacatg ccggagctgg aagtctactt
     7261 ccactaccgt tatctcgatg tcagcaccct gaaagagctg gcgcgccgct ggaagccgga
     7321 aattctggat ggttttacca agcaggggac gcatcaggcg atggatgata tccgtgaatc
     7381 ggtggcggag ctggcttact accgcgagca ttttatcaag ctgtaaaatt tttcctggtc
     7441 acgtaagcgc cggaatccac ttgcggcggc gtgattttga cgctaaatcg aacgtcttgc
     7501 tgataatttc agcacttgag ataaaaacgc aaaaaaaact tttttggggg gttgcagagg
     7561 gaaagatttc tcgtataatg cgcctcccgt aacgacgcag aaatgcgaaa attacgaaag
     7621 caaaattaag tagtacgcgg gaatagctca gttggtagag cacgaccttg ccaaggtcgg
     7681 ggtcgcgagt tcgagtctcg tttcccgctc caaaatttga aaagtgctgc aaagcacaga
     7741 ccacccaatc gcagtatttc gaattgcgac aaggcggcaa ctgaacgaat ccttgggaac
     7801 ttacaaaagt atgtgactaa ggtgagtgaa ggcagccaag gcagtagcgg ttcgaaagac
     7861 gaagatta
//
LOCUS       DAJTPG010000152         7829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTPG010000152 DAJTPG010000000
VERSION     DAJTPG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(60..1274)
                     /gene="rspA"
                     /locus_tag="OO230_003687"
     CDS             complement(60..1274)
                     /gene="rspA"
                     /locus_tag="OO230_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5693287.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1480..1806)
                     /locus_tag="OO230_003688"
     CDS             complement(1480..1806)
                     /locus_tag="OO230_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5693288.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1941..2282
                     /locus_tag="OO230_003689"
     CDS             1941..2282
                     /locus_tag="OO230_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5693289.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2348..2877
                     /gene="speG"
                     /locus_tag="OO230_003690"
                     /pseudo
     CDS             2348..2877
                     /gene="speG"
                     /locus_tag="OO230_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2880..3590)
                     /locus_tag="OO230_003691"
     CDS             complement(2880..3590)
                     /locus_tag="OO230_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5693290.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3698..4003
                     /locus_tag="OO230_003692"
     CDS             3698..4003
                     /locus_tag="OO230_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5693291.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4202..4522
                     /locus_tag="OO230_003693"
     CDS             4202..4522
                     /locus_tag="OO230_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5693292.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5082..5660
                     /locus_tag="OO230_003694"
                     /pseudo
     CDS             5082..5660
                     /locus_tag="OO230_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6346..7335)
                     /locus_tag="OO230_003695"
     CDS             complement(6346..7335)
                     /locus_tag="OO230_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693293.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7420..>7829)
                     /locus_tag="OO230_003696"
     CDS             complement(7420..>7829)
                     /locus_tag="OO230_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693294.1"
                     /translation="YPLSLAIKTLIPGYLSGLPSTSREAMELLEALAEAGVTIGNFFS
                     NDLMTAYGRRQMNKRAEAERIAKEQRLQAERMREENMTDAEWQKELQRREQVKAERRT
                     YGESLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT         2040 a         2002 c         1890 g         1897 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcaatta tcttactcct
       61 tacctgttcc acagcgtgcc atcttccaga cgtgcgactg gtagataagc aggttcatag
      121 ggatatttcg ccgccagctt ttcatcgaat tcgataccaa gacccggttt gtctcccgga
      181 tgcatatagc cgttatcgaa agtccagttg tgcgggaaga cttcgagcat ttgttcggaa
      241 taacccatgt attcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca
      301 gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc
      361 gaagcaaaat cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg
      421 cggatataat cgatgagttg ctcttcaatc agttgtttgc agtcccagat gctgttgaag
      481 acttcaccca ctgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcttgg
      541 ttttccgctg gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca
      601 aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta
      661 aaaccaaact tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc
      721 gtcgaccaca gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct
      781 ttacctttcg acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg
      841 gctttgaatc ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga
      901 ccggtggtat ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa
      961 agcggcatgt tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg
     1021 gccgacatcg taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata
     1081 tcttcgatac ggtgcgcatc gcgaccaata agccgcggac aaagatgatc ctgcaaataa
     1141 gaggccacgg aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc
     1201 tcagtggtga tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc
     1261 tttacgatct tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa
     1321 ctgagctact gccatacaag tatgaagatc gaaaaaagcc ggagtgatca caaaaaaagg
     1381 cgtatatttg cgctgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga
     1441 cacttcctcg tatcttattg ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc
     1501 aacaatgatc aacattccgc aaagcgcaat caacgcaccc gtccagtcat aaagacttag
     1561 tttcacgcca tccacaacgc gcagccacat caatgccgtg cagacataaa cgccaccata
     1621 agccgcgtaa acacgcccac tcgccgcagg atgcaacgtt aacaaccaga caaacagcgc
     1681 cagtgaaatc cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca
     1741 gggcagaaag catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt
     1801 aatcatcttt gtcacttatt gacatcatgt atagttatag ggcgacataa tatcatcaat
     1861 ataaacatcc tcctggtacg ttttgcgttc gcagtggatg gtgtagaatc acctttaatc
     1921 attcatacag ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt
     1981 ttctgctgcc ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg
     2041 tgattgagtc tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat
     2101 ggaatgacac gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata
     2161 aagccgacgc cgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct
     2221 actgggagcc caatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct
     2281 aacctgttat tgatttaagg aatgtaagga cacgttatgc caagcgccca cagttaagct
     2341 acgcccgctg gagcgtgaag atttacgcta tgtacatcaa ctcgacaata acgccagtgt
     2401 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga
     2461 taagcgcata ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa
     2521 gccggtctgg tggagctggt ggaaattaac catgttcatc gccgggcaga atttcagata
     2581 attatctccc cggagtatca ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg
     2641 gactatggct tcaccgttct caatctctat aagctgtatc tgatcgtcga taaagagaat
     2701 gaaaaagcga ttcacattta ccgcaagctt ggctttacgg ttgaaggtga attgatgcat
     2761 gagtttttta ttaatggtca atatcgtaat gccattcgca tgtgtatatt ccagcatcag
     2821 tatctggcag agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat
     2881 taataataat cgatcgtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct
     2941 tcatcaacaa tgatcagctg acagtccact ggattggcgt gactgtcata ttcacagctc
     3001 tgttttacat taccaactcg ttgattattt agtaaagtaa ccgctgtgta atctaatttt
     3061 ttgatcggat ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt acttttcgtg
     3121 gttttaccca gcggataccc ctgatcatca tagcgatatt ccatctgcat ttgtttgctg
     3181 ctggctttaa tcacgaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat
     3241 tctgctagct ggcattttcc ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg
     3301 taataattgg cgtccagtac cagagcgacc acggtattat tttccagatc cagtaattcg
     3361 agtgaatcaa aacagccttc ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct
     3421 tgctcatcca ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg
     3481 aattcattag agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca
     3541 catcctgtga ggaatatcgc gagtaagcat ggtagtaatt tgtatttcac aacagtcacc
     3601 agagagtaga gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct
     3661 atgcttaaag aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc
     3721 cgcgcttctg ctcgccctcg cctcacccgc ggtactcgcc gcgccgggat cttgtgagcg
     3781 catacagagc gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac
     3841 gttaagtatt gtacccaatg accaggttga tcagcctgat tcccaggtcg tcgggcattg
     3901 cgctaatgat acgcataaaa ttctctacaa ccgcaccacc agcggtaacg tctctgctcc
     3961 cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag taacacatta tcgactgaac
     4021 gccggatatg acacatccgg cgatttgaac atacaatata atctcacctt attactcata
     4081 cccttctatt gatgtggatt aataattctt aacccaaaat gggtagactc cctctattgt
     4141 tagcgcgcta aatattcaat gtataaacat ttatataaca ataaagaaca gggagtgagt
     4201 tatgtccaaa aatgaccgaa tggtgggcat cagccgcaga acactcgtta aatcaaccgc
     4261 gacaggttct ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc
     4321 agcggcagcg gtacaacagg ccagcgaaaa agtgatctgg ggttcctgtt ccgtcaactg
     4381 tggtagccgc tgtgcacttc gtctacatgt taaagataat gaagtgacct gggtggaaac
     4441 tgacaatacc ggcagcgatg agtacggcaa ccatcagggt acgcgcctgt ttgcgcggtc
     4501 gctccatccg ccggcgtatt aatcatcccg atcgcttgaa tgacccaatg aaacgcgtgg
     4561 gtaaacgcgg tgaaggcaaa ttcgaacgga ttagctggga tgaagccctg gatactatcg
     4621 ccagtagcct gaaaaaaacc gtcgaacaat atggcaatga agctgtatat attcagtact
     4681 cttcggggat tgttggcggc aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac
     4741 gcctgatgaa ctgctacggc ggttcactca atcagtatgg ctcctacagc accgcgcaaa
     4801 tttcctgtgc catgccctac acctacggca gtaatgatgg caacagcacc acggatattg
     4861 aaaacagcaa gctggtcgtg atgtttggta acaacccggc agaaacccgc atgagcggtg
     4921 gtggcatcac ttatcttctt gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta
     4981 tcgatccgcg atataccgat acggctgccg gtcgtgaaga ggaatggctc cctattcgcc
     5041 cgggcaccga tgccgcgctg gttgcgggta ttgcctgggt attgattaac gaaaatctcg
     5101 ttgatcaacc ttttctcgat aaatactgcg tcggctatga cgaaaaaacc ttacccgcag
     5161 atgcacccaa aaatggtcac tataaagcct atattcttgg tgaaggtgac gataaaacag
     5221 caaaaacgcc gcagtgggct tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac
     5281 tggcgcgtga aattggcaca acaaaacccg cttatatctg ctagggctgg gggccgcaac
     5341 gccaggcaaa cggcgagctg actgcacgcg ctattgctat gttacctatt ttgacgggca
     5401 atgtcggcat cagcggcgga aatagtggcg cgcgtgaatc gacctatacc attacgatag
     5461 aacgcctacc ggtgctggat aatccggtca aaacgtcaat ctcctgcttc agctggacgg
     5521 atgctatcga tcatggcccg caaatgacgg cactccgcga cggtgtccgg ggcaaagata
     5581 aactggatgt gcccattaag ttcatctgga actacaagga cttgaagcgg acccttagta
     5641 ggcctgaggt cctgcattga gtcaagacct tgtgtcattc tttgtgccag acttgtgtca
     5701 ttagacggga tgtctagacg gctggtcagg accctccggt ggcctcggtc tggacttgag
     5761 gcgggctatg ggggaagtct gctggcttaa tgtattagat ggtcattcac atttttctgt
     5821 cagaaaccat cggtcccact gtgagtacaa acctaagccc ccagaagccc acccacagcc
     5881 ttagacagag tgtcgaatac attcacaccg agtcgaccgg agaggaccat atagaggccc
     5941 acagagagga acacgagttt atagaccaga gtcaggaggg accttaggtc ttcggaagtc
     6001 atggacataa agcccccccc tatcttctat tgcactaccg cactagacaa ctagtacaaa
     6061 acaaaggccc agaccattgg tccagaccgt agttagttaa tcacagtgac aagccaccac
     6121 ctgaagtcct gctgatattc agtccagact tacctctgct catggtctta cctccctcgg
     6181 tctcctgacc ttgggtatcg actacagctt gctgagcgtc atctggagga gctacaggag
     6241 gagctacagg aggcgggacc gaagccccac cttgagtccc accaccacct gtcaggccct
     6301 tggcgatgga cctgaagcct ttactaatgc tcttacccat tggtcttacg cttcggtctt
     6361 ggtgaacttc aggcgtttga ctacaggcag gacggcagta gctgagccag tgcccatctt
     6421 gatggtcgta gcagcttcag cgacaacgaa cactttaccg aggatttcca agtcgtggga
     6481 ctgagagtcg ccatcacgtg aaaggacgac cgtaatattc tcaccgtcgt gacggaagca
     6541 gccgaagctc agggccgtga agcgtttagc cagcatgaat acacccttgt cttcaccgtt
     6601 gtaataggcg atgtctgtcc ccagcatatc gacgatagcg tcttcaccgg agaagccttg
     6661 cttagcagcc agacgggaca gtgctttcag ttggctctca tgagcgataa taccgtagtc
     6721 agacacctca gaaccgaaag aagactctgt gttctcatac agagcgtcca tgatagcgtc
     6781 tgccttagcg gtcaggctac cagtcaggac aggaagctct acggtcttca gcagcttaga
     6841 ggattccaga acgtcgacca tgcgagcgtt gatggtacgg accacattct cacagatgtt
     6901 catagcgtcc tgctgaagtg tgccctgagt gaatccggtg gatgacttgg cgtcccagag
     6961 cttggtctgc tcagagatga gaatagcctg ctcgattggt tcgaccacga tttcttgtcc
     7021 agacggaacg gtcacgactt caggtcgctt gccaccacgg acaggaacga ccacagagcg
     7081 gtcagccaga gtgtgagcaa tgattggggt cggtaagctg ttacgggtcg gcaggacttc
     7141 tacagggaac gggatgttct cttgagcgtc tttaagtaca ccgttcagca tagccttaac
     7201 cgtcttagct aactggaggg catagacctg cgggtcagcg ttagtcggca gaccttcatt
     7261 gacctgaatg ttgtcggtat cggtgatgcg tactggctca atgccaaact ggaaagcgtc
     7321 tttaatcgta gtcataataa tcaatctcct aaattagttt aaatactgtg agtcctcaaa
     7381 actgaggagt ccctaacatc gggtcaggga ctgtgtgttt tacaagctgt ccatccagtt
     7441 accgcctgac tcaaggtcag cgacaatagc ggcacgggag cgaccagcgg aatgggtagc
     7501 actgcggagg ctctcaccat aggtccggcg ttcagctttg acctgttcgc ggcgctgtag
     7561 ctctttctgc cactctgcat cggtcatgtt ctcttcacgc atacgctcag cctgaagtcg
     7621 ttgctcttta gcgatacgct cagcttcagc tcgtttgttc atctggcggc gaccataggc
     7681 ggtcatcaag tcattactga agaagttgcc aatggtcacg ccagcttcag ccagagcttc
     7741 cagtaactcc attgcctcgc ggctggtaga cggcaggcct gacaggtagc ccggaatgag
     7801 ggtcttgata gcgagcgaca gcggatagg
//
LOCUS       DAJTPG010000153         7541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTPG010000153 DAJTPG010000000
VERSION     DAJTPG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO230_003697"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO230_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO230_003698"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO230_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5693295.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO230_003699"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO230_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO230_003700"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO230_003700"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5693296.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO230_003701"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO230_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5693297.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO230_003702"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO230_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5693298.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO230_003703"
     CDS             complement(6510..7202)
                     /locus_tag="OO230_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5693299.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1832 c         1986 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc cccccccccg cgccgctgag aaaaagcgaa g
//
LOCUS       DAJTPG010000154         7488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTPG010000154 DAJTPG010000000
VERSION     DAJTPG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..1236
                     /gene="waaA"
                     /locus_tag="OO230_003704"
     CDS             <1..1236
                     /gene="waaA"
                     /locus_tag="OO230_003704"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5693300.1"
                     /translation="PLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSV
                     GETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNR
                     FLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGYAKLGKFVRRLL
                     RRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHR
                     PVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYIT
                     RSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLM
                     GPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRSFYGRHAVEVLYQNQ
                     GALQRLLQLLEPYLPPKTH"
     gene            1244..1723
                     /gene="coaD"
                     /locus_tag="OO230_003705"
     CDS             1244..1723
                     /gene="coaD"
                     /locus_tag="OO230_003705"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5693301.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1762..2571)
                     /gene="mutM"
                     /locus_tag="OO230_003706"
     CDS             complement(1762..2571)
                     /gene="mutM"
                     /locus_tag="OO230_003706"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5693302.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2669..2836)
                     /gene="rpmG"
                     /locus_tag="OO230_003707"
     CDS             complement(2669..2836)
                     /gene="rpmG"
                     /locus_tag="OO230_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5693303.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2857..3093)
                     /gene="rpmB"
                     /locus_tag="OO230_003708"
     CDS             complement(2857..3093)
                     /gene="rpmB"
                     /locus_tag="OO230_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5693304.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3310..3978)
                     /gene="radC"
                     /locus_tag="OO230_003709"
     CDS             complement(3310..3978)
                     /gene="radC"
                     /locus_tag="OO230_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5693305.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4150..5370
                     /gene="coaBC"
                     /locus_tag="OO230_003710"
     CDS             4150..5370
                     /gene="coaBC"
                     /locus_tag="OO230_003710"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5693306.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5348..5806
                     /gene="dut"
                     /locus_tag="OO230_003711"
     CDS             5348..5806
                     /gene="dut"
                     /locus_tag="OO230_003711"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5693307.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            5913..6509
                     /gene="slmA"
                     /locus_tag="OO230_003712"
     CDS             5913..6509
                     /gene="slmA"
                     /locus_tag="OO230_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5693308.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6546..7187)
                     /gene="pyrE"
                     /locus_tag="OO230_003713"
     CDS             complement(6546..7187)
                     /gene="pyrE"
                     /locus_tag="OO230_003713"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5693309.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7253..>7488)
                     /gene="rph"
                     /locus_tag="OO230_003714"
     CDS             complement(7253..>7488)
                     /gene="rph"
                     /locus_tag="OO230_003714"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="RNase PH; tRNA nucleotidyltransferase; forms
                     hexamers in Bacillus subtilis; phosphoroltic 3'-5'
                     exoribonuclease; involved in maturation of tRNA precursors
                     and removes terminal nucleotides near CCA acceptor arms of
                     mature tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5693310.1"
                     /translation="VSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAE
                     GEPFTHEELLTLLALARGGIESIVATQKAALAN"
BASE COUNT         1747 a         2047 c         1868 g         1826 t
ORIGIN      
        1 ccgctgatct ggatacggct ctgggtgcgc ggacgtaagg ctccggccta tcgaaaacgc
       61 tggggtgaac gttacggttt ttaccgccat ccgctaaaac caggcggcat tatgctgcac
      121 tccgtctccg tcggtgaaac tctggcggcg atcccgttgg tgcgcgcgct gcgtcatcgt
      181 tatcctgatt taccgattac cgttacaacc atgacgccaa ccggttcgga gcgcgtacaa
      241 tcggctttcg ggaaggatgt tcagcacgtt tatctgccgt atgacctgcc cgatgcgctc
      301 aaccgtttcc tgaataaagt cgaccccaaa ctggtgttga ttatggaaac cgaactatgg
      361 cctaacctga ttgcggcgct acataaacgt aaaattccgc tggtgatcgc taacgcgcga
      421 ctctctgccc gctcggccgc aggttatgcc aaactgggta aattcgtccg ccgcttgctg
      481 cgtcgtatta cgctgattgc tgcgcaaaat gaagaagatg gtgcacgttt tgtggcgctg
      541 ggcgcaaaaa ataaccaggt aaccgttacc ggtagcctga aattcgatat ttctgtaact
      601 ccgcagttgg ctgctaaagc cgtgacgcta cgccgccaat gggcaccaca ccgcccggta
      661 tggattgcca ccagcactca cgaaggtgaa gagagcgtgg tcattgccgc acatcaggca
      721 ttgttacagc aattcccgaa tttattgctc atcctggtac cccgtcatcc agaacgcttc
      781 ccggatgcga ttaaccttgt ccgccaggct ggactaagct atatcacacg ctcttcaggg
      841 gaagtcccct ccaccagcac gcaggttgtg gttggcgata cgatgggcga gttgatgtta
      901 ctgtacggca ttgccgatct cgcctttgtt ggcggttcac tggttgaacg tggtgggcat
      961 aatccgctgg aagctgccgc acacgctatt ccggtattga tggggccgca tacttttaac
     1021 tttaaagaca tttgcgcgcg gctggagcag gcaagcgggc ttattaccgt taccgatgcc
     1081 actacgcttg caaaagaggt ttcctcttta ctcaccgacg ccgtttaccg tagtttctat
     1141 ggccgtcatg ccgttgaagt actgtatcaa aaccagggcg cgctacagcg tctgcttcaa
     1201 ctgctggaac cttacctgcc accgaaaacg cattgaggtt gttatgcaaa aacgggcgat
     1261 ttatccgggt actttcgatc ccattaccaa tggtcatatc gatatcgtga cgcgcgccac
     1321 gcagatgttc gatcacgtta ttctggcgat tgccgccagc cccagtaaaa aaccgatgtt
     1381 taccctggaa gagcgtgtgg cactggcaca gcaggcaacc gcgcatctgg ggaacgtgga
     1441 agtggtcggg tttagtgatt tgatggcgaa cttcgcccgt aatcaacacg ctacggtgct
     1501 gattcgtggc ctgcgtgcgg tggcggattt tgaatatgaa atgcagctgg cgcatatgaa
     1561 tcgccactta atgccggaac tggaaagtgt gtttctgatg ccgtcgaaag agtggtcgtt
     1621 tatctcttca tcgttggtga aagaggtggc gcgccatcag ggcgatgtca cccatttcct
     1681 gccggagaat gtccatcagg cgctgatggc aaaattagcg tagcgtttat gccggatggt
     1741 atgccatccg gctcgcatga attacttctg gcactgccga caataaaacg ttgcccgctg
     1801 cgcatgttta gtcgccacaa tcggcgtacc gcacacccga cacggctcac cttttcgccc
     1861 gtaaacctgc aattcctgcg cgaaatagcc cggtttacca tcactttgca gaaaatcttt
     1921 cagcgttgta ccaccctgct caatcgaacg cagcaacacc gctttaatca cccgagctaa
     1981 caattcacac tctgccagcg acagtgatga cgccagccga tccggatgga tccccgccgc
     2041 aaacagtgat tcgctggcat agatattccc tacccctacc accagcttgt tatccatcag
     2101 ccacggttta atcgccgttt ttttcttcgc gcacttctga tgcagatact caccattgaa
     2161 atcgtcgcta agcggctccg gtccaagatg ggccagcaca ttatgccctt ccagctcttt
     2221 ggtccacagc caggcaccaa agcggcgcgg atcggtgtag cgcagcactt taccgttgct
     2281 catcaccaaa tccacatggt catgcttttc agggggaagt tcttctggaa ggatgcgcag
     2341 gctgccagac atccccaaat gaatgataat ccagccctca ggcagctcca gcagcagata
     2401 tttagcccgc cgctgcacgc taagcactgg ttgatcgctt aatcggtaga tctcttctga
     2461 aaccggccag cgcaagcgtc cgttgcgcac caccgcatga agaatggttg caccaacgag
     2521 atgcggttct atgccgcgac ggctggtttc aacttcgggt aattcaggca tagcatctcc
     2581 aggaatgaac agatgcaatc aatatggggg caagcagata acaaaaaacc ccgccggagc
     2641 gaggtttttc gttacatcaa agcgagaatt atttgatttt cgcttctttg tagatcacgt
     2701 gctgacgaac aactggatcg aattttttca gttccagttt ttccggctta gtacgtttgt
     2761 tcttcgtagt ggtatagaag tgaccagtac cagcagaaga aaccagcttg attttctcac
     2821 gaataccttt agccatgatt tatttcctct aagtacttag tacttttcgc cacgggcacg
     2881 cagttcagcc agaactgtat cgatgccttt tttatcgatt acacgcatac ctttagcaga
     2941 tacgcgcagg gtgacaaaac gcttctcgct ctcaacccag aaacggtgag agtgcaggtt
     3001 cggcaggaaa cggcgtttag tcgcgttcag tgcgtgggaa cggttgttac cggtcaccgg
     3061 acgcttgcca gtaacttggc agactcggga catgtctatt ctccaaaaat caaattagct
     3121 cgagcttcgt atggggtatt ggcgcctcgt caggctttac agcccggtca tcgcagttct
     3181 atgtgaactc tcgattgcca ggcccaaatg ccaaacccga gattctcaaa ggtggcgtag
     3241 tatacgctga ctcagcgatg tgctcaagtc ccgaacagac aaagatcccg aaggatcgcg
     3301 catagcgggt taaatccagc cgcgttcggc aaaagaaaca tactctccac gcccaatcac
     3361 gatatggtcg agcacgcgta aatccatgaa ctgacaactc tttattatcc gttcagtaat
     3421 gagtttatcc gctttactgg gttcagcaca acccgaaggg tgattatgtg caaggatcag
     3481 cgccgaggcg tttattttta tcgcttcgcg gataatttcc cgaggatgga cttcaacatg
     3541 gtttagcgtg ccggaaaaaa gacggctatg cgttataacc cggtgttgtg agtcgagaaa
     3601 gatcaccata aagatctccc gctcctcacc cgtgagctgg ctttgtaaaa attcccgcgt
     3661 catctccggg ctgagtaaag ggctttcttc acgcatccgc acgttgtagt aacgtcgcgc
     3721 cagttcagca atccccttta actgggcaaa tttcgctacg ccaattccat gaacaccact
     3781 aaattgttca tattcagagg ttaacaagcc ataaagagag ccgaaattct ccagcatctc
     3841 ttttgccagg gttaatacat ctttaccgcg cgttccggta cgcagaaata gcgccagcag
     3901 ctcgacatcc gttaaggcgc taataccaaa cttcagcatt ttttcgcgcg gcatcaacag
     3961 ctgtgcattg tttttcacct tcacctcctt tgtggtgctc gcatcctgtc atagccgttc
     4021 cgttaaatcg acggccagtt ttcaatcctg gaaagagcct cgcaaagtga atctcaggtg
     4081 aaggcacgac aacaaaagta ttgtgataaa atcgccaact tctggtgtca cccaacagga
     4141 aaaatcatca tgagcctggc cggtaaaaaa atcgttctcg gcgttagcgg cggtattgct
     4201 gcctataaaa cccctgaact ggtgcgtcgt ttgcgcgatc gcggggccga cgtccgcgta
     4261 gccatgaccg aagcggcaaa agcctttatc accccactta gcttgcaggc ggtttctggt
     4321 tatcccgttt ccgacagtct gcttgacccg gcagccgaag ccgctatggg ccatattgag
     4381 ctgggtaaat gggctgattt agtgattctc gcccctgcca cggcagattt gattgcccgt
     4441 gttgctgccg gaatggcgaa tgacctggta tcgacgattt gtctggctac acctgcgcct
     4501 gtagccgtgc tccccgccat gaaccagcag atgtaccgtg ccgctgccac gcagcataat
     4561 ttagaggtgc ttgcttcccg tggtttgctc atctgggggc cagacagtgg cagtcaggct
     4621 tgtggtgata tcggtcctgg acgaatgctc gatccgttaa ccattgtgga tatggcggtg
     4681 gcgcattttt cgcccgtcaa cgacctgaaa catctgaaca ttatgattac cgccggtccg
     4741 acgcgtgaac cgctcgatcc ggtgcgttat atctctaatc acagctccgg caagatgggt
     4801 tttgctatcg ccgccgctgc tgcccgtcgt ggcgcgaacg tcacgctggt atcaggtcca
     4861 gtttcactac cgacgccacc gtttgttaaa cgtgttgatg tgatgaccgc gctggaaatg
     4921 gaagccgccg tgaatgcttc tgtacagcag caaaatattt ttatcggttg cgccgccgtg
     4981 gcggattatc gcgcagctac cgtggcccca gaaaaaatca aaaagcaggc cacgcagggt
     5041 gatgaattaa caataaaaat ggttaaaaac cccgatatcg tcgcaggcgt tgccgcacta
     5101 aaagaccatc gaccctacgt cgttggattt gccgccgaaa caaataatgt ggaagaatac
     5161 gcccggcaaa aacgtatccg taaaaacctt gatctgatct gcgcgaacga tgtttcccag
     5221 ccaactcaag gatttaacag cgacaacaac gcattacacc ttttctggca ggacggagat
     5281 aaagtcttac cgcttgagcg caaagagctc cttggccaat tattactcga cgagatcgtg
     5341 acccgttatg atgaaaaaaa tcgacgttaa gattctggac ccgcgcgtta ggaaggaatt
     5401 tccgctcccg acttatgcca cctctggctc tgccggactt gacctgcgtg cctgtctcga
     5461 cgacgccgta gaactggctc cgggtgacac tacgctggtt ccgaccgggc tggcgattca
     5521 tattgccgat ccttcactgg cggcaatgat gctgccgcgc tccggattgg gacataagca
     5581 cggtatcgtg cttggtaacc tggtaggatt gatcgattcc gactatcagg gccagttgat
     5641 gatttccgtg tggaaccgtg gtcaggacag cttcaccatt caacctggcg aacgcatcgc
     5701 ccagatgatt tttgttccgg tagtacaggc tgaatttaat ctggtggaag atttcgacgc
     5761 caccgaccgc ggtgaaggcg gctttggtca ctctggtcgt cagtaacaca tacgcatccg
     5821 aataacgtca taacatagcc gcaaacattt cgtttgcggt catagcgtgg gtgccgcctg
     5881 gcaagtgctt attttcaggg gtattttgta acatggcaga aaaacaaact gcgaaaagga
     5941 accgtcgcga ggaaatactt cagtctctgg cgctgatgct ggaatccagc gatggaagcc
     6001 aacgtatcac gacggcaaaa ctggccgcct ctgtcggcgt ttccgaagcg gcactgtatc
     6061 gccacttccc cagtaagacc cgcatgttcg atagcctgat tgagtttatc gaagatagcc
     6121 tgattactcg catcaacctg attctgaaag atgagaaaga caccacagcg cgcctgcgtc
     6181 tgattgtgtt gctgcttctc ggttttggtg agcgtaatcc tggcctgacc cgcatcctca
     6241 ctggtcatgc gctaatgttt gaacaggatc gcctgcaagg gcgcatcaac cagctgttcg
     6301 agcgtattga agcgcagctg cgccaggtat tgcgtgaaaa gagaatgcgt gagggtgaag
     6361 gttacaccac cgatgaaacc ctgctggcaa gccagatcct ggccttctgt gaaggtatgc
     6421 tgtcacgttt tgtccgcagc gaatttaaat accgcccgac ggatgatttt gacgcccgct
     6481 ggccgctaat tgccgcgcag ttgcagtaat atgacgccgg atgacttttc atccggcgag
     6541 tttctttaaa cgccaaactc ttcgcgatag gccttaaccg ccgccagatg ttccgccatt
     6601 tccggcttct cttccagata ggcaatcaga tctttcaggg tgatgataga gatcactttg
     6661 cagttgtaat cacgctcaac ttcctgaatc gccgaaatct cgccgcgccc gcgttcctga
     6721 cgatcgagcg aaatcaacac gccagcaagc gtcgcgccat tggcctgaat aatctccatc
     6781 gactcgcgaa tcgccgttcc ggcggtgatc acatcatcta ccagcattac gcgtccttgt
     6841 aacgcgctac caaccagatt gccgccttca ccgtggtctt ttgcttcttt gcggttaaag
     6901 cagtacggca ggtccaggtc gtgatgctcc gccagtgcca cagcggttgt ggtggcaatc
     6961 gggatccctt tgtaagcagg gccaaacagc agatcgaact caatgccgga atccaccaac
     7021 gcttcagcgt aaaaacggcc taacagtgcc agatcgcgcc cggtattaaa cagcccggcg
     7081 ttgaagaaat aggggctttt gcgcccggat ttcagcgtaa actcgccaaa ctttaacacc
     7141 tgcttgctaa gcgcaaattc aataaactgg cgctgatatg atttcatgcc ttcgctcctc
     7201 atcttacttt tctacagaca aaaaaaaggc gactcatcag tcgccttaaa aatcagtttg
     7261 ccagcgccgc cttctgcgtc gctacaatgg attcgattcc ccctcgggcc agcgccaaca
     7321 aggtgagtag ctcttcatgg gtgaacggct cgccttctgc cgtcccctgc acttcaatga
     7381 tgcgcccgtc ttcggtcatc actacgttca tgtcggtctc tgcggctgag tcttcaacgt
     7441 attccagatc gcaaattgct tcaccgttta caataccgac agaaactg
//
LOCUS       DAJTPG010000155         7199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTPG010000155 DAJTPG010000000
VERSION     DAJTPG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            53..1543
                     /locus_tag="OO230_003715"
     CDS             53..1543
                     /locus_tag="OO230_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5693311.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1595..2752
                     /locus_tag="OO230_003716"
     CDS             1595..2752
                     /locus_tag="OO230_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5693312.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2718..2840)
                     /locus_tag="OO230_003717"
     CDS             complement(2718..2840)
                     /locus_tag="OO230_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693313.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3043..4728
                     /locus_tag="OO230_003718"
                     /pseudo
     CDS             3043..4728
                     /locus_tag="OO230_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4766..7126
                     /locus_tag="OO230_003719"
     CDS             4766..7126
                     /locus_tag="OO230_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5693314.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         2120 a         1572 c         1526 g         1981 t
ORIGIN      
        1 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga
       61 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      121 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa
      181 atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta acggctatgt
      241 ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
      301 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
      361 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
      421 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
      481 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
      541 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
      601 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
      661 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
      721 accaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac
      781 agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
      841 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
      901 tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac
      961 ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg
     1021 taattatgac aagctgattt ccgcaatgga tttctaccca acagctcttg atgcagccga
     1081 tatcagcatt ccaaaagacc ttaagctgga tggtgttccc ttgctgccct ggttgcaaga
     1141 taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt
     1201 tgatgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga
     1261 cgattacccg cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa
     1321 taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac
     1381 ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca
     1441 aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgagg taaatcagga
     1501 gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc
     1561 ggatttttcc gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag
     1621 ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac
     1681 tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat atatcgaagc
     1741 gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga
     1801 ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa
     1861 tacattacaa acgaatggca ttttattgaa taatgaatgg tgtgcctttc tcaaagaaca
     1921 tgaatttctg gttggtatct cgatcgatgg cccccaggag ttacatgacc gttacagacg
     1981 cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc
     2041 atatcaagta gagtttaata cgttaaccgt cattaataac gttaatgtcc attatcctct
     2101 tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct
     2161 agaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat
     2221 tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg
     2281 ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt
     2341 tttggggaat gggcatacaa gttgtatttt ccaggagtcc tgcaaggata atctggttgt
     2401 tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg
     2461 aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa
     2521 acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc
     2581 taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa
     2641 aatccttttt tcaaccatgg taccttatat gaacgccatg gttgagttag ctaagaacag
     2701 agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc
     2761 tcttttaact ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt
     2821 cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg
     2881 cgtcagggca ttagcaatac ccttgattat caatagcata accataacca ctgaaaatta
     2941 aaaatttcta tatatttcat attgttaaat gatgcttaaa taaatttcat cagtaaatca
     3001 ttgccataga taatcattgg atggcacaat caattttcgc taatgataac cattcccatt
     3061 acgttttgta tgttgatagc aaagtatctc tgtctgctga agcccttttg gctgcgtaag
     3121 aataataaaa cttcagtgtt gttaatcatt attatcctcg caatgatcct tggcgtggtt
     3181 aaaattcagg tctggttaaa cgactggaat aatgattttt tcaatgcgct aagccagaaa
     3241 gaaaccgata agctttggca gctcgttctt tggttccctg ccctgctggg gatctttgta
     3301 ttaatatccg caaacaaaac atggttaatt aaactactga ctattcgctg gcgcgaatgg
     3361 ttaacagatt actaccttaa tcgctggttt gcagataaaa actactactt cacgcagatc
     3421 tacggcgagc ataaaaatac agataaccct gaccaacgta tcgctgaaga cattctctta
     3481 ctgatcaaca aaacgctcag tctttcattt ggcttcatcc agtcacttag catgctgatc
     3541 acctttaccg ttattctttg gcaaagcgcg ggtacgctct cattcactgt tggtggaaca
     3601 gaatggaata tccaggggta tatggtctat accgtcgtgc tcattgtgat cggtggaact
     3661 ttatttaccc ataaggtggg taaacgtatt cgtccgctta atgtggaaaa acaacgtagc
     3721 gaagcgacat ttcgaactaa tcttgtgcag cataataagc aggcagaact gattgcgcta
     3781 agcaatgctg agtctctaca acgccaggag ttgcgcgaca attttcatac cattaaagag
     3841 aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt actggcaaaa tatttattcg
     3901 cgctcgctta gcgttcttcc ctactttctg ttattgccgc agtttattag cgggcagatc
     3961 aatctgggcg gactgatgaa atcgcgccag gcatttatgc tagtatcgaa caatttaagc
     4021 tggtttattt ataaatatga cgaacttgct gaactggctg cggttatcga tcgcttgtat
     4081 gagttccatc aactcactga atagcgccct acgaataagc ctaaaaattg ccaacatgcg
     4141 gtacaagtgg ctaatgcgag tattcgtacg cctgataata agatcatatt agagaacttg
     4201 aactttcatg tttcgccagg caaatggcta ttactgaaag gctactctgg tgcgggaaaa
     4261 accacactgc ttaaaacatt atcccactgc tggccgtggt ttaaaggtga tatttcttct
     4321 cctgctgaca gttggtatgt gtcacaaaca ccgttaatca aaagcggctt actgaaagag
     4381 attatttgta aagcacttcc cctgcccgta gacgataaat cgttgagcga agtactgcat
     4441 caggttggtc tgggaaaatt ggctgcgcgt attcatgacc acgatcgctg gggagatatt
     4501 ctttccagcg gcgaaaaaca acgtatcgcc ctggcacgat taattttacg acgtccgaaa
     4561 tggatatttc ttgacgaaac tacctctcat cttgaggaac aagaggctat acgcttactg
     4621 cgtttagtgc gtgaaaaact acccacaagc ggcgtcatta tggttacaca tcaacccggt
     4681 gtctggaacc tggctgatga tatttgtgac attagcacgg ttttataaca acaataaaag
     4741 ccctgctgta cggagatata aataaatgaa gcgagttctt attcctggcg tcattttatg
     4801 tggcgctgat gtggcgcagg ccgtcgatga caaaaacatg tacatgtatt tttttgaaga
     4861 gatgacggtc tatgctcctg tccctgtacc cgtaaacggc aacacgcatt acaccagtga
     4921 aagcatcgag cgtttaccga ccgggaatgg caatatcagc gatctgctga gaaccaaccc
     4981 tgcggtacgc atggattcaa cgcaaagtac ctcgttgaac cagggagata ttcgccctga
     5041 gaaaatctct attcacggtg cgtcgcccta ccagaatgcc tatttgattg acggtattag
     5101 tgcaactaat aacctgaacc cagcgaatga gtccgatgcc agtagtgcaa ccaatattag
     5161 cgggatgtca caggggtatt atcttgatgt cagcttactg gacaatgtga cgctttatga
     5221 cagttttgtg ccggttgaat ttggtcgctt caatggcggg gtaattgatg caaagatcaa
     5281 acgcttcaac gctgatgata gcaaggtgaa attgggttat cgcactacgc gtttggactg
     5341 gttaacatcg catatcgatg agaataacaa gagcgcattt aatcaaggtt cttcaggaag
     5401 tacctatttc tctccagatt ttaaaaagaa cttttatacc ttgtcgttta atcaggagct
     5461 ggctgataac tttggcgtta ccgccggttt atcgcgccgc cagtctgata tcacccgcgc
     5521 ggattatgtt tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa acgttatcga
     5581 tactgcattg agcaaattta cctggtttgc cagcgaccgc tttacccacg atttaacctt
     5641 aaaatatacc ggctccagcc gtgattataa taccagcacc ttcccgcagt ctgatcgcga
     5701 aatgggtaat aaatcctatg gtctggcatg ggatatggat acgcaactcg catgggccaa
     5761 actacgtacc accgttggtt gggatcatat tagtgattat acccgtcacg atcatgacat
     5821 ctggtacacc gaactttcat gtacatatgg tgatattaca gggcgttgta cccgtggcgg
     5881 attaggacac atttcccagg ctgtagataa ttacaccttc aaaacacgcc tggactggca
     5941 aaaattcgcc gtgggtgatg tttcgcatca accctacttc ggcgcggaat acatctattc
     6001 cgatgcatgg actgaacgcc ataaccagtc tgaatcctat gtgattaatg ctgcaggaaa
     6061 gaaaactaac cataccattt accataaagg taaaggcagc ctgggaattg acaactacac
     6121 gctgtatatg gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg gtgtgcgtta
     6181 tgactatgac aactatctgt caaaccacaa tatctccccg cgctttatga cggaatggga
     6241 tatttttgct gatcaaacct caatgattac cgccggttat aaccgttact atggcgggaa
     6301 tattcttgat atgggattac gtgatatccg caatagctgg acggaatcgg tatcaggtaa
     6361 taaaaccctg acgcgttatc agaatttgaa aacgccttat aacgatgaac tggcaatggg
     6421 attgcagcaa aaaatcgata agaacgttat tgcacgcgca agtgaagcgc atgatcaaat
     6481 cagcaaaagc agtcgtaccg acagcgcgac taaaaccacc attactgaat ataacaacga
     6541 tggcaaaacc aaaacgcatt cgtttaacct cagttttgaa ctggccgaac ccctgcatat
     6601 ccgccaggta gatattaacc cgcaaattgt ctttagctat atcaagagca agggcaactt
     6661 gtcgttaaac aatggttatg aggagagcaa taccggtgat aaccaggtgg tttataacgg
     6721 taatctggtc tcttacgata gcgttccagt ggcagatttt aataacccat taaagatctc
     6781 cttaaacatg gatttcacgc atcaaccgag cgggttagtg tgggcgaata cgctggcctg
     6841 gcaagaagcg cgtaaagctc gcattatcct tggtaagaca aatgcgcaat acatcagcga
     6901 atattcagat tacaagcagt atgttgacga aaaactggat agcagcctga cctgggacac
     6961 ccgcttgtcc tggacgccac aatttctgaa acaacaaaac ctgacgatca gtgccgatat
     7021 tctcaatgta ctggatagca aaaccgctgt tgatacaacg aacaccggtg tggcgaccta
     7081 cgccagtggc cgtactttct ggcttgatgt cagcatgaaa ttttaatagt tcggatttta
     7141 cttaaccggg taaattcgcc cggttttcgc atggagataa ttatgagaaa cctctgttt
//
LOCUS       DAJTPG010000156         7154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTPG010000156 DAJTPG010000000
VERSION     DAJTPG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..264)
                     /locus_tag="OO230_003720"
     CDS             complement(<1..264)
                     /locus_tag="OO230_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR5693315.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFE"
     gene            445..1869
                     /gene="sbcB"
                     /locus_tag="OO230_003721"
     CDS             445..1869
                     /gene="sbcB"
                     /locus_tag="OO230_003721"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5693316.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1912..2139)
                     /locus_tag="OO230_003722"
     CDS             complement(1912..2139)
                     /locus_tag="OO230_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5693317.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2153..3211)
                     /gene="yeeE"
                     /locus_tag="OO230_003723"
     CDS             complement(2153..3211)
                     /gene="yeeE"
                     /locus_tag="OO230_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5693318.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3390..4748)
                     /gene="plaP"
                     /locus_tag="OO230_003724"
     CDS             complement(3390..4748)
                     /gene="plaP"
                     /locus_tag="OO230_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5693319.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4738..4800)
                     /gene="yoeI"
                     /locus_tag="OO230_003725"
     CDS             complement(4738..4800)
                     /gene="yoeI"
                     /locus_tag="OO230_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5693320.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4961..5944)
                     /locus_tag="OO230_003726"
     CDS             complement(4961..5944)
                     /locus_tag="OO230_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5693321.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5990..6814)
                     /locus_tag="OO230_003727"
     CDS             complement(5990..6814)
                     /locus_tag="OO230_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5693322.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6897..7151)
                     /gene="yoeB"
                     /locus_tag="OO230_003728"
     CDS             complement(6897..7151)
                     /gene="yoeB"
                     /locus_tag="OO230_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5693323.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1940 a         1863 c         1676 g         1675 t
ORIGIN      
        1 ttcaaaataa gtgcgtggat ccgttcctga gaaatagagc tttcgcaagt cagcagtgat
       61 ctgtctggca tccttcaaag acagggatgg gtatcgacca agcccaagtc gattaggctt
      121 gccatgccag cgatagcggt actggaactg gatgaccccc ttcggtgaaa ttcgtacgct
      181 gaggccatcg gcatcagcca cttcttgtgg gcccgaatat ggtttaccat aaatagtacg
      241 cagttttgtg tcgcttatag ccataaataa tatttggtac gctcagaaat aatagtttgg
      301 cacacatctg gtacacaata tcacatggac aacagcgcac agtaaacaac tatatttgac
      361 gttcgtagac atactcaggt gaaaaaaggg ttgctttcga gttataacag acagttaatt
      421 aactacagtg gacaatgcta gacaatgaca gacacagata aacaacctac cttcctcttt
      481 cacgattacg aaacctttgg cacgcacccc gcgttagatc gccctgcaca gttcgcagcc
      541 attcgcaccg atgacgaatt caatgtcatc ggcgaacccg aagtctttta ctgcaagccc
      601 gcggatgact atttaccccg gcctggagca gtattaatta ccggtattac cccgcaggag
      661 gcacgggcga aaggagaaaa cgaagccgcg tttgccgccc gtattcactc gctttttacc
      721 gtaccgaaga cctgtattct gggctacaac aatgtgcgtt tcgacgacga agtcacacgc
      781 aacgtttttt atcgtaattt ctacgatcct tacgcctgga gctggcagca tgataactcg
      841 cgctgggatt tactggatgt tatgcgtgcc tgttatgccc tgcgcccgga aggaataaac
      901 tggcctgaaa atgatgacgg tctaccgagc tttcgccttg agcatttaac caaagcgaat
      961 ggtattgaac atagcaacgc ccacgatgcg atggctgatg tgtacgccac tattgcgatg
     1021 gcgaaactgg taaaaacgcg tcagccacgc ctgtttgatt atctctttac ccatcgtaat
     1081 aaacacaaac tgatggcgtt gattgatgtt ccgcagatga aacccctggc tcacgtttcc
     1141 ggaatgtttg gggcatggcg tggcaatacc agctgggtgg caccgctggc gtggcatcct
     1201 gaaaatcgca atgccgtaat tatggcggat ttggcaggag acatttcgcc attactggag
     1261 ctggatatcg acacattgcg cgagcgttta tataccgcaa aagccgatct tggcgataac
     1321 gccgccgttc cggttaagct ggtgcatatc aataaatgtc cggtactggc ccaggcgaat
     1381 acgctacgcc cggaagatgc cgaccgactg ggaattaatc gtcagcattg cctcgataac
     1441 ctaaaaattc tgcgtgaaaa tccgcaagtg cgtgaaaaag tggtagcgat attcgcggaa
     1501 gccgaaccgt ttacaccttc agataatgtg gatgcacagc tttataatgg ctttttcagt
     1561 gacgccgatc gtgcagcaat gaaaattgtg ctggaaaccg agccgcgtaa tttaccggca
     1621 ctggatatca cttttgttga taaacgaatt gaaaagttgt tgttcaatta tcgggcacgc
     1681 aacttcccgg ggacgctgga ttatgccgag cagcaacgct ggctggagca ccgccaccag
     1741 gtcttcacgc cagagttttt gcagggttat gctgatgaat tgcagatgct ggtacaacaa
     1801 tatgccgatg acaaagagaa agtggcgctg ttaaaagcac tttggcagta cgcggaagag
     1861 atcgtctaat ttgaagccgc cgccgctggt acggcggagg acaatgttga gtcaggcttt
     1921 ttgaacggtg atgctccacg ctgcatcgcc aatctgctga taatcggtga tggcatgtcc
     1981 ttcttctgcc gcccactgtg ggattgcttc agttgcctgg gtgcaatcaa actcaattac
     2041 caactcatcg ccgctggcca tttctgccag tgctgctttt gcttctatga gtggaaacgg
     2101 acagacctgc gtcaccacat ccagcttttt aatcgccatc tttacacctt acttaatttg
     2161 ccgcagcagt tgccagtcgc gccttacgct gcggtcgaac atacacaagc caggacgcag
     2221 tccagactcc gagaatcata aatacaaggc caatccagcc ctgccaggtc atcattgcag
     2281 tcataaccag tccattaccg atagagcaac cacctgcaat actggcaccg aagcccatca
     2341 gtacaccacc taacccgcta cgtaatgttg tttgtgcatc agctgcgcga acgcggaact
     2401 cacggctcgc tttggctgca ataaatgcct ccacgaagat ccctaacacc aggaaaacgc
     2461 cccagttaat gtatttcacg tcacccgcga ccagaaattg aagaatattg gccgttgggg
     2521 aagtgactcc taacccaaac atgcgtccgg ttgcttcact caggggccag gctaaaagcg
     2581 caatcaaacc gatgagtaca gcagtgacaa agggatgcca gcgtttttca aacagaatat
     2641 gagcgatccc ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc ttcttcaact
     2701 ctttcatcac cacccagagc gttatcacca gcaaaacggt aaccaacggc cacacagaca
     2761 aattaaaagt ctcagcaata gagttatgtt cagtagtgta gtgctgcaag gtttgattta
     2821 aaccactggc gtgtggagaa cgcatcaccg cactcatcac catataagtg aaaagcgcga
     2881 tccaactgcc gatcaatccc tcacccgcgc gataccaggt cccggtggca catccacccg
     2941 ccagaacaat tcccagcccg aaaagatacc cacctataac agtaccaagc cacgggaacg
     3001 ccccggcttc gtaagtcagt aggcccgcct gaattaacgc aaaaaccccc acgctttgtg
     3061 ccgaaattgc aatcagcaag gcgtaaaaca tgcgattatt tttcgcgata tacatatcgc
     3121 gaaaaccacc agtcaggcaa aaacgcccac gctgcatgac aaatcccagc agagcaccac
     3181 aaattagccc gcttaatatc attgaaaaca taaattaatt aaccagatga atgttaatgg
     3241 ggaaattttt catgactggt ggaatgcaga ccaataacca aattctctaa attagaacaa
     3301 atggttatta atgaggattt cgataaaaaa gaataaaaaa accggagcca aatgttcatt
     3361 cgactccggt ttattattag aaaagatgat tatgctacgt cttcgtactg cggaaccgga
     3421 ttacggaagc tcttggtgac gcaagccagg taaatcagac cgatagcagc ccagatcaga
     3481 cccagaacca ttgagctttc ttccaggtta acccacagcg caccaacggt cagcgcacca
     3541 cacatcggca ggaacagata ctggaagtga tctttcagcg tcttgttacg cttctcacgg
     3601 atccagaact gcgagatgac cgacaggtta acaaaggtga acgccaccag cgcaccaaag
     3661 ttaatcagcg ccgttgccat taccaggtcg aagttgattg ccagcagggc aatcgcgcca
     3721 accaggatga tgttcatcgc cggagtacgc catttcgggt gtacataacc gaagaagctt
     3781 ttcgggaaca cgccgtcacg acccattacg tacatcagac gcgctacgcc tgcgtgcgcc
     3841 gccataccgg acgccagtac ggtaatggtg gagaagatca gcgcgccaac ctggaacgct
     3901 ttacccgcaa cgtacagcat gatttcaggc tgtgacgcat ccggatcttt aaagcgagag
     3961 atatccggga agtacagctg caggaagtaa gttgcaaaga tgaagatcat gccgccaatc
     4021 agcgcggtca ggaaaatcgc acgcgggatc acgcgctctg catctttggt ttcttccgac
     4081 agattgctga tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc cccggtaatc
     4141 atcgggataa catgcgcatc gccagaccag aacggacgcg tgctcgccag cgtaccagcg
     4201 ccttcacctt caaatacgcc ataaacaacc atgcccagaa tcaccgcgat cagcactacc
     4261 tgcaacacga cgattacagt gttgacgttc gctacggatt tcagactacg caggttaaag
     4321 gcggtcataa aggccaccag cgccaccaca aacatccacg atggaatgga aggcaccaga
     4381 gcttcaaaat agattttcgc cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc
     4441 agagaagacc aacccaccat aaagccgaca gtcgggctaa tggatttctg ggcgtaagtg
     4501 tatgcagagc cagcagaagg atagcggcga accagcttcc cgtagctcag agccgtaaac
     4561 aggatcgcaa tcaatgcgaa cgcataggct gtcggcacat gaccatccgt gaggcctgaa
     4621 acgataccaa atgtatcaaa cagcgtcatc ggctgcatat aggctagacc catcattaca
     4681 accggaacta acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg agtaacgtta
     4741 tgcgacatgg ttattctcct ttacggtgat aaccgtcgcg taagcaaaaa attgccccat
     4801 ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta tccggtacga
     4861 agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac gctaattaaa
     4921 cgtcggtttt taatcatttt ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac
     4981 aagagaatga gaggcttgtc ataataattt ttctttgaat ggctgatttt ccgtcatcag
     5041 attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatctcgc caccagccgc
     5101 tggcaagccc tgcggtctgt tcattccagc caatccagac aggttcaaag agagtttggg
     5161 cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgcgcc agataccgtg
     5221 gtaaatagcc gcagccaagg ccgctaattt gcagctccag tttggtttta aaatcgaaaa
     5281 cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag
     5341 tatcccccac cacaatcgcc cgatatcgtt taatgatacg acggtttaac ggttcttctt
     5401 cctgggctaa cggatgatga ggcgcgaccg caaagacttg ttcaagatcg cccaaccgcg
     5461 agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacgata atatccgctc
     5521 gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac ttcaggcgcg
     5581 tcacgctatg atgttgataa aaagcttcaa tgagaggcgc aagcagagaa aaaggaaacg
     5641 tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg atcgcctgct
     5701 tttccagttc tcgcacggta tgcagaactt cccgcccttt ctctaataac atttttccgg
     5761 tgcgggtgaa tttagcgcgg tgtccgctac gatcaagcaa ttgaatattt aggtcgcttt
     5821 ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg gctgacgccg
     5881 ccgcaaaact gccctctttt tctatggcat cgaggatcat caacacatcc agcagtggtt
     5941 tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt tactccaacg
     6001 gcattaccag cggatcggga tactggtatt caaatcccag ttcattacaa atccgactgc
     6061 catcaataat cttacctttg ccgctgtcca gactatttct gaactgcgga ggttgcagcc
     6121 ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc agggtgagct ggcgcacata
     6181 tattatagat gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg ccaataacat
     6241 cttctaaatg gactaaatta acaccatgtt caccatcagg ggcggttttt ccggcaaaga
     6301 agcgtcccgg atgacgtccc ggtccgacca ggcccgcaag acgcagaata tcgaccgaag
     6361 taccgggtaa attgtgcagc cagtcttcga gttcttctaa tactcgtcca ctgttggtta
     6421 ccggattacg cggggtagtt tctttaaccg tgccttgcgc gtcgccatag acagatgttg
     6481 agctggtaaa aataattcgg ggaatacgat gggccagcgc gctatccact aactcttgta
     6541 ccgcttgtaa atagaactca tcgccggggc cgctacgacg tgccggaagc gtaatgacca
     6601 gcgcatcggc atccataagg gcatccagat cgtcagaatc gcaaactaac tcaggctcca
     6661 tgcgaagcag atagctatca atgccactca ttcgggccgc ttcgacacca tcttgtgtgg
     6721 ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc ggcataccta
     6781 accaccctaa cccgacaatt gcgacctttt tcatccgtta tctcctgata caccttagat
     6841 ctataaggct acgctagcgt atcaaaactg acaattcatt ctatgaatga atctgttcaa
     6901 taatgataac gacatgctgc aatgagcagt gaatcatcag taaccgcgta taccagacgg
     6961 tgctcctctg taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag gggttctggt
     7021 ttccccttac cttcaaatgg tgttctgcgg gtatctttga taagttcatt gatcttttta
     7081 acaattcgct tatctgtttc ctgccagtac agataatcat cccatgattc ctcagaccag
     7141 attagtttca ctca
//
LOCUS       DAJTPG010000157         7133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTPG010000157 DAJTPG010000000
VERSION     DAJTPG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..1003
                     /gene="ydfJ"
                     /locus_tag="OO230_003729"
     CDS             <1..1003
                     /gene="ydfJ"
                     /locus_tag="OO230_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5693324.1"
                     /translation="TLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCG
                     TLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDS
                     NQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDA
                     LMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMV
                     ALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCT
                     MTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            1092..2552
                     /locus_tag="OO230_003730"
     CDS             1092..2552
                     /locus_tag="OO230_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5693325.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2588..2791)
                     /gene="ydfZ"
                     /locus_tag="OO230_003731"
     CDS             complement(2588..2791)
                     /gene="ydfZ"
                     /locus_tag="OO230_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5693326.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2969..3655)
                     /gene="ydfH"
                     /locus_tag="OO230_003732"
     CDS             complement(2969..3655)
                     /gene="ydfH"
                     /locus_tag="OO230_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5693327.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3744..4490)
                     /gene="ydfG"
                     /locus_tag="OO230_003733"
     CDS             complement(3744..4490)
                     /gene="ydfG"
                     /locus_tag="OO230_003733"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5693328.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4627..6672
                     /gene="dcp"
                     /locus_tag="OO230_003734"
     CDS             4627..6672
                     /gene="dcp"
                     /locus_tag="OO230_003734"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5693329.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6717..>7133)
                     /locus_tag="OO230_003735"
     CDS             complement(6717..>7133)
                     /locus_tag="OO230_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5693330.1"
                     /translation="LTTAESCTGGKLASALCAAEDTPKFYGAGFVTFTDQAKMKILSV
                     SQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYGGPEGGEDGTPAGTVWFAWH
                     IKGQNYTAVMHFAGDCETVLALAVRFALAQLLQLLL"
BASE COUNT         1762 a         1700 c         1839 g         1832 t
ORIGIN      
        1 gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg ccggtacgat
       61 gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg tggctatggg
      121 aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct tcattctcag
      181 taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg ttgtcgtgat
      241 ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg agaaggttaa
      301 cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc agagcaaatc
      361 cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt taattcagac
      421 tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc caacagatgc
      481 attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt ggttatccga
      541 taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg tgctggcgtg
      601 gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta tggttgcact
      661 gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca ttaccatggc
      721 agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag aaattggtgg
      781 tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca tgacggaatc
      841 ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa tctctgcgct
      901 gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg cggaagatca
      961 accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca atcccttctc
     1021 ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat atttaaattt
     1081 aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct gtttatgatc
     1141 gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac cgtgcgcatc
     1201 agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga tattatgagg
     1261 tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat aatctttata
     1321 ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc gttaaaaaag
     1381 ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt gaaccgcaaa
     1441 tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg gcgaccggac
     1501 agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac cagccgaaaa
     1561 cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga cttcccgcat
     1621 ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt gacgttgtca
     1681 cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat aacgtgactt
     1741 tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg ctggcgaaaa
     1801 tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct ttccgccagt
     1861 gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg ggagccgaac
     1921 tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac ggcagtcatt
     1981 cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac tgtatggaag
     2041 atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg ccgacgctga
     2101 aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc tatagcaacc
     2161 cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa ttgccacagc
     2221 ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat ctgctggcgc
     2281 tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga aatccgatag
     2341 aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt gccgaaggga
     2401 aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat ttgccagaca
     2461 atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg catggcgcga
     2521 aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat cggcggcatt
     2581 cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc tcttcacaac
     2641 cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct tcagtatcaa
     2701 tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg ccagtggtga
     2761 ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg atttacagtc
     2821 gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt ttttgttttt
     2881 gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg aaaagcctct
     2941 catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg ctgttttcct
     3001 ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt gcacgttcta
     3061 ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg cgtaacagca
     3121 tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg gtcgctttca
     3181 ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg agttgatgga
     3241 agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca atgcgttgct
     3301 ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc gccgcccgac
     3361 gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc tgggccatgg
     3421 aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg ttttccgcca
     3481 gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca gaaacttctt
     3541 tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga cgaagaatac
     3601 gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg gtcattcgtt
     3661 cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa gcaataaccc
     3721 ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc atagctttgg
     3781 gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt tgacacccac
     3841 cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata ggttttttcc
     3901 gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc caccagaccc
     3961 ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag attcaggcta
     4021 aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata cggccagctg
     4081 cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat acccggtaag
     4141 acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt ttcccagtct
     4201 tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt atttaccagg
     4261 atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat agcggcgcgg
     4321 ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt taactcctgc
     4381 aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat aaaacgacga
     4441 gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat ctcactgttc
     4501 ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg taatactggt
     4561 ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg cgttcaggag
     4621 aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta tctggctcct
     4681 cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg aatgcaacaa
     4741 aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt caacaatact
     4801 attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt cttttttgcg
     4861 atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt ttccgctgaa
     4921 ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg ggtagatgct
     4981 gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct ggtggaggtt
     5041 attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa agcaaaatta
     5101 aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg gttacttgca
     5161 gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg aatgagtgag
     5221 caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa caaatggctg
     5281 attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga tcgtgcgacg
     5341 cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc caatgatacc
     5401 cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact gcttggtttt
     5461 cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga agcagcactc
     5521 aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga attagcctcc
     5581 atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg ggactgggca
     5641 ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca gctcaagcca
     5701 tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa tcagctcttc
     5761 ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt tcgtgtgtgg
     5821 gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt cttcgcccgt
     5881 gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac gcttaatgaa
     5941 acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg tgagcctgcg
     6001 ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac gctgcacggc
     6061 ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg tgattttgtc
     6121 gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt cgctcgctac
     6181 gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa aatgcgtaat
     6241 gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc acttctcgat
     6301 atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga ttttgaattg
     6361 cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta tcgcagcagt
     6421 tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta tctgtggacg
     6481 caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt aacgcgtgaa
     6541 aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga tctggaacgc
     6601 ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca acatcgtggc
     6661 ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca gcctggttat
     6721 agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa taccgtttcg
     6781 cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg ccacgcgaac
     6841 cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta gccggtaatg
     6901 gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc tgctgccact
     6961 ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag gattttcatc
     7021 tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt atcttcagct
     7081 gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt taa
//
LOCUS       DAJTPG010000158         7085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTPG010000158 DAJTPG010000000
VERSION     DAJTPG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(238..1140)
                     /locus_tag="OO230_003736"
     CDS             complement(238..1140)
                     /locus_tag="OO230_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5693331.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1219..2172)
                     /gene="lsrR"
                     /locus_tag="OO230_003737"
     CDS             complement(1219..2172)
                     /gene="lsrR"
                     /locus_tag="OO230_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5693332.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2421..3956
                     /gene="lsrA"
                     /locus_tag="OO230_003738"
     CDS             2421..3956
                     /gene="lsrA"
                     /locus_tag="OO230_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5693333.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3950..4978
                     /gene="lsrC"
                     /locus_tag="OO230_003739"
     CDS             3950..4978
                     /gene="lsrC"
                     /locus_tag="OO230_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5693334.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4978..5970
                     /gene="lsrD"
                     /locus_tag="OO230_003740"
     CDS             4978..5970
                     /gene="lsrD"
                     /locus_tag="OO230_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5693335.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5982..7005
                     /gene="lsrB"
                     /locus_tag="OO230_003741"
                     /pseudo
     CDS             5982..7005
                     /gene="lsrB"
                     /locus_tag="OO230_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1651 a         1789 c         1851 g         1794 t
ORIGIN      
        1 ccaggctatt tctccatgca caggccaatt atgaaagagg agaggcttaa gttcttgaac
       61 cctgaacgaa taacgactac cagaaaagca cttcgcatca ctcagacatg atttaacata
      121 atatacatta tgcgtagtaa tattgtagta gctaaattga gatgtccgtc tctgactaaa
      181 tagacatgtc cgcgctacag tctcaccaca gcgatcactg ctttcaaagg atgggactca
      241 tgacggcata cggaacggag taatcggcaa ctgatgccgg gcctcgctga gcgtgcactg
      301 cttattatcc gtgaacgtgc cgccaagaac cgcggtttgt gccggacgca caacgcctaa
      361 cccaaggcaa ccaagctgca cgtcgcctcc tccaacgacc accagagtgc ccgccttcag
      421 accgcagagt tccgccgctt gtgaacttac cacgcccagc aatgtgccgg tttctttgac
      481 aggagaaaga atatcggcac gcaggccagc catatccagc aatgcaggtt tccaggcacg
      541 ggtggttaga tcaagaagtc ccgtggtgcc agcgttagag ggatccaccg ccagttcgcc
      601 gctgagcata taggccagcc agtcgctgat catggtgatg gttgatgcct gacggtaaat
      661 atcggaacga tggtgcgcca gccaaagtaa tctggggatg gcacttaaag ccagtgtttg
      721 tccggtcgct cgataaactt cgttttcaaa ggtattgttg tgcagttctt taagttcgct
      781 aacttcgcgt gccgctctgg catccacatt ggcgcaggcc cagatcggtg ttccttcatt
      841 gttatataaa acaatgcctt cacgcataga acatgccgaa acggcagcga tatactccgg
      901 gtctatgccg gcgttgtgca gcgcctggcg catacactca cacgccagtt gccagttttt
      961 gttgagatca aattccatag aaccaggaac gtccggtact gccagatgcc gccactccgc
     1021 ctgtcccact gctatttgat tgccttccag gtcgaatatc acagcccgaa tacttccggt
     1081 gcctgcatcc agcgccatca ggtagtactt tgattctgaa ggggtaaaga gtcgagccat
     1141 tagattatcc tcggcttatg cgttctgtct gttcctctat acgttctcca tcattcccgg
     1201 taataaggtc atgcaaattt aactacgtaa aatcgccgct gctgtgtcct gatcggtaac
     1261 cagtgcgttg atataaccgc ctttcattgc agcggcaatt gcttcagctt tattttctcc
     1321 ccctgccacg ccaacccgga cgggtatggt cttcagcgcg cttaaaggta agccaatcag
     1381 ttcgttatgt attttgatat ccgtgacaac gtcacctttt gcatcaaaaa agtagcctaa
     1441 aatgtcgcca accgcccctt ttcggccaat cattaactgt tcgccctggc tgatataacc
     1501 ggagcgaatg attgtcgcgt cgtcttgttg actcacggct ccaatgccga caatcgccac
     1561 atccgctgct tgcgcggcta gcagaacatc tttgacgcaa ttttcatttt ttagcgtacg
     1621 ggcaatgtca gcggaggatg cccgcaacgg agccggaata atattcacac tgcacgccgc
     1681 gttaagctgc ccgatacccg tcatataaga accgacgcca ccggagagcg tgaccaggcg
     1741 aatttgctgt gacgaaataa aaccacttaa gcgttgcagc gtattcatgg ttgcctcgcc
     1801 aaaaccaatc gccagcatct gttgtggttg aagtaaactc atcaacatat gcgccgcgcc
     1861 tatccctagt cgcccgccga catcagcatc cgcaagccca gggatcaccc ggacatgttg
     1921 cagcgaaaac tgacgacgta attgagtttc atattccaga cagccttcaa agcgagaatt
     1981 aatctgtacg cgaataatgc cggactgatg ccctttctcc agcaagcgcg acactttcaa
     2041 acgggtcagg ccgagacgat cgctgatctc gctctgggtc agcccgtcgt gatagtaaaa
     2101 ccacgcgatc cgcgcgacct gttcttcttc acacattccc tgttctgaaa ttgccgaatc
     2161 gttgattgtc ataattcatt cttcactttg aacatattta aatctttaat gcaattgttc
     2221 agttcttgcg catttatatc tgtgatggca accacagttt gactctacga gcatgaacaa
     2281 acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta ttcgtcggaa atatgtgcaa
     2341 tgtccaccta aggttatgaa caaattaaaa gtagaaatac atttgttcaa aactcacctg
     2401 caaaactgaa cgggggaaat atgcaaacga gtgatacccg cgcgttaccg ctactttgcg
     2461 cccgctcggt ttataaacag tattcagggg tcaatgtcct gaaaggcatc gattttacgt
     2521 tgcatcaggg ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa
     2581 tgaagattat tgccggtatt acccctgctg atagcggtac gctggagatt gggggcaaca
     2641 actacgccag attaacgcca gttcatgctc atcagctggg catttatctc gttccccagg
     2701 aaccgctgct tttcccaagc ctgtcgataa aagaaaacat cctgtttggg ctggcaaaaa
     2761 aacagctctc catgcagaaa atgaagaact tgctggcggc gctgggctgc cagtttgatc
     2821 tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg
     2881 ggctgatgcg cgactcgcgg attctgatcc tcgatgaacc taccgcctcg cttacccctg
     2941 cggaaaccga acgcttgttt agtcgcttgc aagagctgct tgctactggc gtgggtattg
     3001 tttttatctc gcataagctg ccggaaattc gccagattgc cgatcgaatt agcgtgatgc
     3061 gcgacggaac catcgcctta agcggcaaaa ccagcgaact gtctaccgac gacattattc
     3121 aggccatcac cccagcggta cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg
     3181 agttacctgg taaccgccca caacatgccg tcggaacgcc ggtgctgaca ctggaaaatc
     3241 tgactggcga aggcttcagg aatgtcagcc tgacgctcaa tgccggagaa attctgggcc
     3301 tggctgggct ggtgggagcc ggacgcacag aactggccga gacgctctat ggtctgcgta
     3361 ctttgcgtgg cggacgcatt atgctgaatg gtaaagagat caataaatta tccactggag
     3421 aacgtttact gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc ggactgaatc
     3481 tcgatgcttc gctggcctgg aacgtctgcg cccttactca taaccttcgt ggattctggg
     3541 cgaaaaccgc gaaagataat gccaccctgg aacgttatcg tcgggcgctg aatattaaat
     3601 ttaaccaacc ggaacaagct gcacggacat tatccggtag caaccagcaa aaaatcctca
     3661 ttgccaaatg cctggaagct tcgccgcaag cattgattgt cgatgagccg acgcgcggcg
     3721 tggatgtctc ggcccgtaat gatatctacc agctgttgcg cagcatcgcc gcacagaatg
     3781 tggctgtgct gtttatctcc tctgatatgg aagagatcga actgatggca gatcgtgtgt
     3841 atgtgatgca tcagggcgaa attacccact ctgcactgac cgggcgcgat attaatgtcg
     3901 agactattat gcgcgttgcc ttcggcgata gtcagcgtca ggaggcatca tgctgaagtt
     3961 tattcagaac aaccgtgaaa tcacggcatt gctggcggtg gtgctgctgt ttgtattacc
     4021 cggttttctc gaccgccagt atttaagtgt gcaaacgctg accatggttt atagcagcgc
     4081 gcaaatcctg atcctgctgg caatgggcgc gacgctggta atgcttacgc gcaatattga
     4141 tgtttcagtg ggttcgatta ccggaatgtg cgcggtgctg ttggggatgt tactgaacgc
     4201 aggatattca ctacctgttg cctgtgtcgc gactttactg cttggtttgc tcgcgggatt
     4261 tttcaacggt gccctggtcg cgtggctaaa gatccctgcc attgttgcca cccttggcac
     4321 gttagggttg tacagaggca tcatgttgct gtggactggc ggtaaatgga ttgaagggtt
     4381 acccgccgaa ctgaaacagc tctccgcccc gctgctgctt ggcatttcag caattggttg
     4441 gttgacgata attctggtgg catttatggc ctggctgctg gcgaagacgg cgtttggacg
     4501 cagtttttat gccacgggcg ataatttaca gggcgctcgt caactgggcg ttcgtacgga
     4561 agccattcgc attgtggcat tttcgttgaa cggctgcatg gcggcactgg cgggaattgt
     4621 gtttgcttcg cagattggtt ttatcctcaa ccagaccggt accgggctgg agatgaaagc
     4681 aattgcagcc tgcgtgctgg gcggcattag tttgctcggt ggttccggtg cgatcatcgg
     4741 tgcggtactc ggcgcatggt tcctgacgca gatcgatagc gtactggtgc tgttgcgcat
     4801 tccggcatgg tggaatgatt ttatcgcggg tctggttctg ctggcggtgc tggtgtttga
     4861 tggacgcctg cgttgtgcgc tggaacgtaa tctacggcgg caaaaatatg cccgctttat
     4921 gacgccaccg ccatccgtta aacccgcttc gtcaggtaaa aaacgggagg ccgcataatg
     4981 cgtattcgct acggttggga actggctctt gccgcactgc tcgttattga gattgtcgca
     5041 tttggtgcaa ttaacccgcg aatgttagat ctcaatatgt tgctgttcag caccagtgac
     5101 tttatctgca ttggcattgt cgctctaccg ctgacgatgg tgattgtcag tggcgggatc
     5161 gatatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt actgtttcaa
     5221 agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc attgtgcggg
     5281 ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat tacgcttggc
     5341 acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg agcgacgggg
     5401 tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct ggatgtgctg
     5461 ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg gctctggctg
     5521 cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg cgtggcgctt
     5581 tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac ggggctggcg
     5641 tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc cgatctcggt
     5701 gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa tatttatggt
     5761 ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata tttgcagcaa
     5821 ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg tgcgctgctt
     5881 atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa agagtggctg
     5941 gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt catcgcttta
     6001 agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat gtgcaggccg
     6061 cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc agcggtggca
     6121 acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac gggccgacag
     6181 aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa ggttataacg
     6241 ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa acgcgccatg
     6301 caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg ccgctcttac
     6361 tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat ggcggcgcgt
     6421 caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac cgttacggac
     6481 caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc aggctgggaa
     6541 attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac cgcagaagga
     6601 atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa cgccctgccc
     6661 gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt cggattcagt
     6721 acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt tggactgtgg
     6781 gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgtattatt gaaaaaagga
     6841 tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga agtctcgcca
     6901 aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact gttaccggag
     6961 cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc attacttaac
     7021 cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt cgtaccgatc
     7081 aaccg
//
LOCUS       DAJTPG010000159         7083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTPG010000159 DAJTPG010000000
VERSION     DAJTPG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(8..886)
                     /gene="xdhB"
                     /locus_tag="OO230_003742"
     CDS             complement(8..886)
                     /gene="xdhB"
                     /locus_tag="OO230_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5693336.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(897..3194)
                     /gene="xdhA"
                     /locus_tag="OO230_003743"
     CDS             complement(897..3194)
                     /gene="xdhA"
                     /locus_tag="OO230_003743"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5693337.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            3609..4364
                     /locus_tag="OO230_003744"
     CDS             3609..4364
                     /locus_tag="OO230_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5693338.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4443..4516
                     /locus_tag="OO230_003745"
     tRNA            4443..4516
                     /locus_tag="OO230_003745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4475..4477,aa:Gly,seq:ccc)
     gene            4651..6261
                     /locus_tag="OO230_003746"
     CDS             4651..6261
                     /locus_tag="OO230_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5693339.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6293..6900
                     /locus_tag="OO230_003747"
                     /pseudo
     CDS             6293..6900
                     /locus_tag="OO230_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1819 a         1712 c         1833 g         1719 t
ORIGIN      
        1 ctgtgattca ttgcaatttt ccccccgccg cggcgacggc ttcgctaatc acttttttgg
       61 tcatcgtctg gatgagatgc agacgaaact ctttactggc ccgccatgaa gaacgcgggg
      121 cgacatcttg caggacagat tcgctgatag cttccagcgt ttgcaggttt aatggcgcat
      181 tttgtgcagt ctgttcggca tgttggcagc gaatcggcgt tggcgcggca acaccaaatg
      241 ccaggcgtaa ttcgctgaaa ttgccgttat ccagtcggca atgtgcggcg cagccaatcg
      301 ttgaaatatc cattgcgtcg cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt
      361 tcggctgtgg cggaaaatga aaggcgacga ggatttcgtc atgctcaaga gacactttgc
      421 ccggcccggt gtgaaagcca ttaatcggga cgaaacgaac accgcgcggg gagtggatct
      481 ccagtttcgc gtcataaatt agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc
      541 aaatatttcc accgtaggta gcgacgttac ggatctgcgg tccagcaatg aacgtggccg
      601 cagcacataa cgccgggaga tgacgttgag ttataggatc ttctattagc tgggtaaatg
      661 tcgttgcaga gccgatacgt agcgagccat cttccgccag cgtaattccc cgcagctccg
      721 ccagattatg aatatcaaca atatggcgat aacggtcatt gtgatggtgg agctgaatca
      781 gtacgtcagt gccaccggcg agcagtttgg cctgcgggtt gtcagccagc aggttgatgg
      841 catcggcaag ggttgctgcg cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa
      901 atcaatcctg ccagatggaa ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg
      961 attgcaacac cggtagccat cttcaccgcg ttacgaatag cagcggcaac aggaattatg
     1021 ggtggctcac ccagtgactt atgtccgtat gcggattgcg gctcattgat ttcgacgaac
     1081 gcgctttcca gttgtggcag atccggcatg gtcggcattt tgtaatccag cagattgggg
     1141 ttacggacca cgccgctttt agcatcgatg atcatctctt caaatagcgc ccagccaatg
     1201 cccattccca ttccgccgtg tacctgacct tctgccagca gtggattaag aatatgccct
     1261 gaatcatgaa cgttgaggat gcggttgatg gtgactttgc acagcgcaat atcgaccgtc
     1321 agatcaacaa aggtacagcc aaacgccggt gggttagtgg tggttttgat ggagctttca
     1381 gcagagagct gcccgccgcg ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac
     1441 gacattaacg gctcttccgg tcgttcaacc agcacgatat ggccttttat cagggtcaaa
     1501 ttcatcgctg actgatgtag catgactgcg gcgtgagcga tgattttctc ttttaataat
     1561 agtgccgcac tgcgcagcgc aggcgcggca acatagctct ggcgtgaggc aaatgcgccg
     1621 ggatcgaacg gcgtaacgtc ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga
     1681 acccccacgg tttctgccac catttgcgag aagacggtgt cggcaccctg accgatttcc
     1741 gtcgcgccgc tttgcacgtt gatggttcca tcctgattca tcagaaggcg cgcgcctgct
     1801 atttctacgc cgacaggcca ggtgttagag gtgtagctaa aacaggcgac gccaacgccg
     1861 cggcgcaaat tgccttgctg gttctggcat tctgcacggc gtttttccca ttcaaagatt
     1921 ttccggcctt tttcaagaca ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc
     1981 ggattagcat ctccttcgcg ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct
     2041 aacgctgtcg cggcgtcatc aagcatagac tcaacggcaa atacgacttg tggcgcgcca
     2101 taaccacgca tcgcaccagc cgaggggagg ttggtatagc aggtctttga actgtaagcg
     2161 taggcacaac gaggataaag gtaagcgact ttattccccc ccgcagaagc gatggagtgc
     2221 ccgtgagatg cataagcgcc ggtgttagac agaacatcca gactataacc tttcaaggtt
     2281 ccgtcgcggt tcacgcccat ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg
     2341 aggaaacact cttcacggct aagggaaact ttcaccggaa tgccgccaag cttgctggtc
     2401 aggaatgccg ccattggctc ttccagtaca tcctgtttat taccaaaacc gccaccgaca
     2461 aacggtttga tgactcgtac gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg
     2521 cgaacaatgt gcgggatctg ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc
     2581 cacgccagcg atgttacgct ttccatgtga caatgttgaa taacgggagt ctgatagtgc
     2641 ccctgtacct ggtagtcggc ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg
     2701 ctttgtttca gtaaattgcc gccgttatgg attggtgcag cgtcttctgt cagcgccgct
     2761 tctggcgtgg tgataacggg taattcttgc cactcaatgc tgaccaattg cgccgctttt
     2821 tctgccgtga gttcatcgcg ggccacgacg atggcaacgg cgtcaccatg atgacgaaca
     2881 tggcgagtta gcagtgcgcg atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca
     2941 tgcccagctg tagcgaatgg aatatcaggc acatcttccc aagtaaaaat cgccagtacg
     3001 cccggtaaac ttctggcttg ttcatcatta atacttacgg cataaccatg tgcgataggg
     3061 ctacgtacat atttcgcgta acacatgccc gccataacat aatcgtcagt atatcgtgcc
     3121 cgcccggtga ccttagcaat ggcatcgacg cgcatgcatg attcacccgt agcggttgct
     3181 tcccgcgctt ccatagaaat cccctcgatt tgaacatttg gttcttatgg caaagattta
     3241 gtctaagcag gcaagattta cgccagaaac gacgataagc gttaggggag gaaggaagtg
     3301 tgatgacgtt aaaaaaacac agatatgata cgaaacagga ctctgaatag ggacagtgat
     3361 acgtatcaat atgagtggcg ataaaatcaa tgtgataaat caggattatt acgcatcagg
     3421 atgggaaata gggtaaaaag tcaggaactt cgatacctct acagcgaata ttttgctgat
     3481 gatacttccc tttgccaaat gaaattaacc tctttctcta ttaccacgtt tttccagaag
     3541 caagagattg ggtatcaaga ggctggctgc tatgatatgg cgtcttgttt tttaagcgag
     3601 gaaagatttt gagtgcggga cgcctgaata aaaaatctct gggtatcgtg atgtttttat
     3661 cggttggact gctattggcg ggctgttcgg gtagcaaatc atccgataca ggaacgtatt
     3721 ccggctccgt ttacaccgtg aaacgggggg atacgctata tcgtatttcg cgcaccacgg
     3781 gaaccagcgt aaaagagctg gcgcgactga acggcatttc ccccccttac accattgaag
     3841 ttggtcagaa actaaaactg ggtggggcga aaagtagcag tagtacacgt aaatcaaccg
     3901 ccaaatcaac gactaaaacc gcatcggtta caccgtcatc agcggtaccg aaatcttcct
     3961 ggccgccagt agggcaacgt tgttggttat ggccaacgac agggaaagtt atcatgccgt
     4021 attcgacagc agatggcggc aataaaggga ttgatatctc agctccacgg ggtacaccta
     4081 tttacgccgc gggtgcagga aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta
     4141 atctcatcat gattaaacac agtgaagatt acattacggc ttacgcccat aatgacacga
     4201 tgctggtaaa taatgggcaa agcgtgaagg ctgggcaaaa aatcgccact atggggagca
     4261 cggatgcggc atctgttcgc ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc
     4321 cgctacgtta cttgccgccg cagggcagca agccaaaatg ctgatggcga attaatcagc
     4381 agtcagcagc gcggcacttg ctaaggagag cgtaaggttt ataatgcctt acgcatctcg
     4441 aagcgggcgt agttcaatgg tagaacgaga gcttcccaag ctctatacga gggttcgatt
     4501 cccttcgccc gctccatgac acttcttaaa gtctactaac ttactgaatc ctaagggata
     4561 cggcttaacg ccttccgata gttttactca ctgtaaccaa cactgtgact aactttgttg
     4621 aagtagtgag cagacctaag gtctcagtac atgctcaagt caagaaccta cctttatcag
     4681 cgtaatggtg tcttttacat ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg
     4741 cttccttctc acaaccgtta taaactagcg tcagtatctt tacggaccaa ggatagacgt
     4801 accgctatgg ctcactcacg gcacatcaag tcagcactta aagcaatcca tgcagacaac
     4861 cctaacgcct cttatgagga gctacgggag cacctgaaga ccattgtcga gtgggaactt
     4921 agtgtcagcc gtgatgacct gaacgacccg gagtcctacc agctttacgt tgaccagtac
     4981 gatgacatta agtccaacct tcgggaagct gtagcgaccg agcgtctgac tgtagaccag
     5041 catcgctata ttaatgacgt tatcggtgtg cttaaggcct gtcaggatag actcaatggc
     5101 gatagctctg gcctattgtc ttaccttgag cctgagactg gcagtctaag accgtccgtc
     5161 tccctatctg tattggcgga acctgaagtc cctgaaccta aagcactgac cctcgcgtct
     5221 ctcattgagc agtacgagca ggagaacgcc cagaactgga agccagcgac cctaagcgaa
     5281 aaccgagcgt cacactctac gctaatcgag atattcgact atctggatat tcaggatgtc
     5341 ggcaaggcga cccgtgcaga tatgctcaga gttcgtgaag tcctccagca gctaccgaag
     5401 aaccgcaagc aacgctttaa gtctatgccg ctgtctgact tgctgaaccg ggagtctaag
     5461 actgactgtc tggacgtcgt taccattaac aataaatatc ttatcaagat ggctgctgtg
     5521 tttaagtggg cggtacgcaa cgacttgata gctaagaacc tgaccgaagg tcttgagctg
     5581 aaagtccctc agcgtaaagc ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg
     5641 caactactgg tcgcagctaa agcgtactcg cagaagacct caggtaagcc ataccattat
     5701 tacgtcaccg ctctggctgc gattacaggt gcgagactta acgaggtggc ccaacttcag
     5761 gttaaagatg tcaggaccac tgaggctggg accgttttta tccatatcaa cgaagacgat
     5821 agcagcctgc cgggtaagag tatcaagaac gcccacagtg accgctgtgt cccgttggta
     5881 gatggtgctt atggttttgt attggctgac tttatgtcac tcgtggaaga ccgtagaaag
     5941 actgagggtg ataatgctat ggtgttcaat ggcctcaagc taatgaagaa cggctacggt
     6001 gagcaggtta gcaagtggtt taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc
     6061 ggcttagctt ttcactcgtt tagacataca gttgcgaccc agttaaaaca acatggggtc
     6121 gagttagcgt atgctcaggc gattatgggt catagctcag ggtctataac ttacgaccgt
     6181 tacgcgaaag aggtcgaggt ggaaacgctg aaagaaaaat tagcggagtc attgtcagta
     6241 aaaaaaatag acggaaagta atcgcatagg gatagcggaa tgtgaggacg taatggtcaa
     6301 agaattttta gttagcaaag aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac
     6361 ttgtttgggg ctggctggaa tggcttcgga agattggcaa cgtgagcatc attgccgcac
     6421 aggtataata tttactgctt ttgctattga ggcaatgttc atcttctata gaaaacaggt
     6481 tgaccccggc tacgataaga cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt
     6541 aaaattgtgc ggtataaaca attatatggg gacaaaacct taccaaatta tcaaagagtg
     6601 tcttgaggta agagacgcta ttgctcacgg cgattcgtat acttcgagct ttaatttctc
     6661 ggcggatcat ttggataacc aagacgatat tgcaaaagtg tcctggcggt aaactctgaa
     6721 caatttcggg gattaactgt tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt
     6781 gaccatgaga tttctgataa tggttacagg ccttctgaag gttaccttcc agcagccgag
     6841 cgcagaagtc tgatgttagc ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga
     6901 gcgataatac acctcactat agaggaatgg acccttaact atcactatag gggagagacc
     6961 ctaagtcccc accttaatag cagtcacgac ccatacacac attcattaag tctcaggctt
     7021 ccggtagcgg accgattgga aaccatccgc agacgcctag tgttaaatca ccttggcctg
     7081 aga
//
LOCUS       DAJTPG010000160         6790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTPG010000160 DAJTPG010000000
VERSION     DAJTPG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO230_003748"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO230_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5693340.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO230_003749"
     CDS             2089..2238
                     /locus_tag="OO230_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693341.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="OO230_003750"
     CDS             complement(3005..3445)
                     /locus_tag="OO230_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5693342.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="OO230_003751"
     CDS             3663..4052
                     /locus_tag="OO230_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693343.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4500
                     /locus_tag="OO230_003752"
                     /pseudo
     CDS             4096..4500
                     /locus_tag="OO230_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4607..5443)
                     /locus_tag="OO230_003753"
     CDS             complement(4607..5443)
                     /locus_tag="OO230_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5693344.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5443..5718)
                     /locus_tag="OO230_003754"
                     /pseudo
     CDS             complement(5443..5718)
                     /locus_tag="OO230_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5793..6266)
                     /locus_tag="OO230_003755"
     CDS             complement(5793..6266)
                     /locus_tag="OO230_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5693345.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6287..6790)
                     /locus_tag="OO230_003756"
                     /pseudo
     CDS             complement(6287..6790)
                     /locus_tag="OO230_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1585 a         1758 c         1771 g         1676 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     4321 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     4381 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     4441 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     4501 ggggtcgttt gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     4561 ggtgagattg atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct
     4621 gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg
     4681 cgttccaagc tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat
     4741 cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac
     4801 aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac
     4861 cgaccagacc gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat
     4921 gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta
     4981 taatcgccgc gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct
     5041 gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag
     5101 atgcggcata cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt
     5161 gctcggcaac gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt
     5221 tctcacgacc gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc
     5281 cgcgcagttc atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc
     5341 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
     5401 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
     5461 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
     5521 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
     5581 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
     5641 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
     5701 atggcaaaca accatatcgt cgctatcaat cgcggaaaaa ggcgtagtgc gcgaagcgaa
     5761 cgaagctttt tgccgtcgat tgcatagctt tgttaaccct ttttccaaat ttgatagcaa
     5821 tagttaatgt ttgaagtaaa gtgttgctca aaaacaactt cgaaggtatt tggaatactc
     5881 gggaagaaaa catccccctc tggctcgatg tcgatcgtcg ataagtggag cgtagaggcc
     5941 atgggtaatg tttctcggta aatttctccg ccaccagaca ctataacgtg accggtgaat
     6001 tcagctagcc tgtccatggc ctcttcgatt gactgaaata caactacatt gtcatcattt
     6061 gatgtccaac ctgagcgggt aacgaccgcg tatttcctat tggggagtgc gcccatagat
     6121 tcaaacgtct tgcgacccac cagaagccac tgattgtagg tcaatgcttt aaaaagtagc
     6181 tgctcccctt tcgcggacca gggtatgtct ggaccgcaac caatcacgcc gtttttcgct
     6241 ttcgcagcta tcaatgatac tttcaaggtt cttatcctgg gttaacggtg cgctcttggt
     6301 cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc ggcgtactct
     6361 tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca tcaacggcgc
     6421 gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat aggctgtgaa
     6481 cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct ccagacagat
     6541 cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc tgttcctcct
     6601 gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg aggcggtcac
     6661 gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag gcatgcccgt
     6721 caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac cagtcagaat
     6781 gcgattcacc
//
LOCUS       DAJTPG010000161         6727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTPG010000161 DAJTPG010000000
VERSION     DAJTPG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(376..810)
                     /locus_tag="OO230_003757"
     CDS             complement(376..810)
                     /locus_tag="OO230_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5693346.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(798..1199)
                     /locus_tag="OO230_003758"
     CDS             complement(798..1199)
                     /locus_tag="OO230_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693347.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1459..2028)
                     /locus_tag="OO230_003759"
     CDS             complement(1459..2028)
                     /locus_tag="OO230_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5693348.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(2773..2916)
                     /locus_tag="OO230_003760"
                     /pseudo
     CDS             complement(2773..2916)
                     /locus_tag="OO230_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(3250..3516)
                     /locus_tag="OO230_003761"
     CDS             complement(3250..3516)
                     /locus_tag="OO230_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693349.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3657..3863
                     /locus_tag="OO230_003762"
     CDS             3657..3863
                     /locus_tag="OO230_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5693350.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            3958..4560
                     /locus_tag="OO230_003763"
     CDS             3958..4560
                     /locus_tag="OO230_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693351.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNINYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            5760..6677
                     /locus_tag="OO230_003764"
     CDS             5760..6677
                     /locus_tag="OO230_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693352.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
BASE COUNT         1663 a         1754 c         1585 g         1725 t
ORIGIN      
        1 gggagtcaca tttttctgca tatctgacac tttggttccc actacttatt tggtggacac
       61 cactttgtct aattcgtcag attctgacca gacggttcag gctgtacgct taccactatt
      121 ttatccctgt tgaatttggt taagccggtg cagacgcgcc gggaaacgca cacctgaacc
      181 ggcacaataa aatacaatac aaccccctgt tactggagat acgaatggaa tgacgaggca
      241 gcctgtcagt cacagggggc tgttggcata aaatcaacgg aaaggccacc aacaccggaa
      301 ttaagctcca cagtccccgc actggcaatc cattcccggg aaaggcatca ccgcaaactg
      361 ctccggaggc aaccattaca gcctctgtaa cggattatca gccctggcat cttccgacgg
      421 cacctcctgc cgcccccacg ccctgctgta taccccggta ccgcagcgtg ggcagcactt
      481 ttccccctca taatcttcgt cacacatcac acaaaaccac tttctcacct tcaccgtata
      541 atccggaacc gtcatgccct gcagatgcaa acgacgggcc tctttctctg ccgggtcaag
      601 ccaggtacat cgcagcagga ttttttcagc gacagtcatc tggtcatgtt caaaccacgg
      661 tggttgtccg tcattgcccc gctgaagtgt taacgggcat ccacaggaca cacagcacaa
      721 aacgtcctgt cggttgtgct tcgcagcttc tttcgccgta acaaaatggc ctttgctgtc
      781 attcgccata taacagttca gaattcgcat atttcctcct gagttgtccg gggaatactg
      841 aatcctccgg tctggcagtg tgtgcagtac tgctccccat aataatcgtg gtgacattgt
      901 ctgcagtgcc agctggcttt acgcaccacg ggtaaggcat ccggtacgaa ttgctgcaga
      961 cgtttaatca actgtatttc tctgcgctcc ggcctgacat aagggcactg ttgaccgtgc
     1021 tctgtcagcc tgtcgtcagt gtgttcaaac cagggaagtt cagtttcgta ttgcggatgg
     1081 tatctgagtg cactgccaca caagtggcag gtgtagttag cataaggtgc ggcctgtgca
     1141 gtacgggcgc ccgtcagacg tccgttgcca tcaagagcga taaaggattt tgcgtacata
     1201 gtcatatacc tcagccgcca gacgacacgc aggcaacagc cgtcccggta cgggcagcgt
     1261 ggtcagggtg tgaatggcgg ttcagttaaa atctggactg gagtttgggt aaagcaggct
     1321 ggttgctgtt acagcactgt ggttgtcaca caatggaaca ctgacgtaaa aaacagtcag
     1381 tgggcgagtg gatacatcgt tgccgccaga caacaaccca tacttttggt ttgccggata
     1441 ttggcgcgcc agtcgtgctc aactccggct acagccaaaa ttgcgctcac cggtgcagtg
     1501 agctttggta tgctccttcg ccagatagtc agcacgttcc agcacttgct gaaagccggt
     1561 gtcatcatta cgcgccagcc acaccgccgg ccaggccgga aaatgcacca gcgtggcatg
     1621 gcacccgaca tccactccca gggcactgca ccacgcctgt ttaatcatcc cggccagaga
     1681 ctccggagcg cggtaatcac cggcacgaca ccaggtatcc cggttgacca gcagcatcag
     1741 gtgatagtgt tttttacctt tgcactcccc aaactcccgg gcccagacgt aatgcagggt
     1801 tgtgggatgc acgcgtttac cttcccgatg cttacgtttc tggtaagcgt cgattcgggc
     1861 tttcagggca ttgatgaagc gggatatcac agctgcgtcc gttgctgccg gtacatccgg
     1921 gagacgcagg tcaacccgca atgccgtcag gcggggatga acattcagtg cgtgccgcac
     1981 cgtctcacga atacgttgct gccagaaggg gttgtatttg taggtcatgg ttaaatctcc
     2041 gtatggttca tacggaatag ccacgtcgta aaaaatacgc agagtccctg aagtgacctc
     2101 cggcaggaca ctgtctcagg cactctgtga taacccgaac ggtcgtctcc ggactgacgg
     2161 ttgaattttc agcgtcgttt tttaatgtaa aaaaccggtt acgggtaagg ctgtgaggag
     2221 ggagtgatgg tgatacgcaa aaagaagtgc agggactgtg gaaacgcgat tactcataat
     2281 acagtgtgtt gcccgtattg tggttctgtc gaccccttcg gctactaccg gaatacagac
     2341 cgtatcgtta ccatactgct ggcgctgatt attgtggtcc tgctgactac tgtcagcgtc
     2401 agtgtgtgta tactgtgtag ctggtgagat aacggttcat gtatcccctg agctgacagt
     2461 gtaccgtgta tattaagcgt aaccgacagg ttctccttca taccatatta aattttccag
     2521 ggattgccgc tgccttattt cgtcccatac aggaaaactc atctgggttt ctgacaaata
     2581 cgcgaaactc acagcccctc aatatcaacc acacgttgta agccggtaat acctgaaaat
     2641 cagcctatta ccgcccacgc ctccggcacc attccctttt gatttcgaat tctttgttgt
     2701 cgctataccg cttaatgttc ccggtcctcc ggtgcagata acaccggagt catcgtagcg
     2761 ctgcttcaat attcaggtga cgtatttcca gacggaagcc ggcgcgcgat cgtaaagttt
     2821 gttcattgtc agttctgcca gccggttgtc aacggcagag ttaaaggctt ccagctccgc
     2881 aagggtaagt ttataacgcg tgtgggaatt cacttttccc gcgtctcctg acgtattacg
     2941 ggctgccact tacctggtgt cccgcaatac agccttagtg ttccgttatc ggggatccgg
     3001 acgcaccgca acaaccgggg aagtatttct ggtttagagc agtgtcaaga gggatttctc
     3061 agcccctcac tcaccggctc agtctcaggg caggagcatt aatacgctca gttcatgtct
     3121 tatacaggaa tgggcacagg gggagctggc gtgtcactgc tgattttacg catcagcgtt
     3181 catgtatctg cactctgata catgaacgat tcacagcgct ctgttaccgg agagatttac
     3241 tctcttagct tactctggca aatcctttac gttactctct gatgacttct tatatttata
     3301 ataccaatca gacagcctta tcccatcggt gatatcagtc tcctcctcgc caggaacggg
     3361 ctgactcact gtaactacct ccgaaaacaa ctctgcaatt atcagaacgc ctgcttctct
     3421 ccctgtcctc acgaaaacta tcccctcttt atcgcgcgtg cgtgcggaag catcttttcg
     3481 caacaaccac ccggtatacc ggtacggctc agccaccgca acctccccgt ttatctcctg
     3541 gcggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg gtgaaggtgg
     3601 cgcgctgttt tcatgaccac accggctggt ttaacaacat ccggaggaac attctcatgg
     3661 ctaccccagt ttcactgatg gatgaccaga tggtcgacat ggcatttatc actcaactga
     3721 ccggcctgac agataagtgg ttttacaggc tcatcaggga tggggccttt ccggctccca
     3781 tcaagctggg ccgcagctcc cgctggctga aaagtgaagt ggaagcctgg ctacaggcgc
     3841 gtattgcaca gccccgcccg taatttctga ccgttatccg ttcacccgca gcagacgtaa
     3901 tccccggcct gccggcggca ttctgctgcc tgttatatcc ccgtgaggaa tatgaaaatg
     3961 aaacaacagt atcagacccg ctacgaatgg ctccacgaaa gctaccagaa atggctgacc
     4021 ggcttcaccc ggcacgccgt atcctggggc gtgtgtcatc cgaatatcaa ctacttccat
     4081 aatctgacgc ccggttgggt gtcattcaat ggtgaaaaac cggagattgc catcgtaccc
     4141 cagagcctgc accggctgat ttatggccct gacaaacggg ccacgccgcc cctggatgat
     4201 gatctgatag tgaatttatg caccagtgaa catctgctgg ttcatcatcc gatgctggaa
     4261 ggcattctcc tgtctgaatg tgaacgtctg aggcagcgtt ctctggcaaa taaactgatc
     4321 agcctcttcc gtcagtttgg tggcacggag ttgcgcctca aactggtctg gctttgctgg
     4381 cttgatttaa tgaccggaaa cagccttgaa gactggaaag agaacctgaa acggaaatca
     4441 gaaaaagagc tggaggaatg gatcatcaac cgtcagaggc agagcgcagc actgacggat
     4501 ctgatggatc agtacgtgct cctggcatac cgcacaacgg ttgacgatag ccgcaactga
     4561 tgccggttgc ttccggaagg tgtgacaaaa tacggaaatg atgatgctcc cggcccccat
     4621 gctcacctgc agagcgtgtt cgtcgtcatc atactgagcc atgacgggcg tcttaagggc
     4681 atcctgtcat tgctgagcag gcaacaccac cagaggagcc acggacaggg atgttcttta
     4741 cagcccgata cacgccaggt tctcagcaga ctgaatgtgc aggtgcggtt attcaccaga
     4801 aatggcatgc tgcggtacat acccatcctg gtgacaatat gtacgtggcg acagcgatcc
     4861 cggcactgtg gaagagactc actcagcctg caggatattc tttccggcat ccgggaatgt
     4921 ccgcctgaat gcaaacagga tgatatttga atccgtaata ctgttctttc atcataaata
     4981 atttatgcag atacagttaa ccgttacagt agccggtttg ttttaccggg aaataatatc
     5041 cgtcagactg ataccggaac atatctgaaa cacgttatga tgttttgtta tcagaaatgt
     5101 tgtatattct tatccgtggc gacagtttaa tgccctctgg tatcacctga taatgtatat
     5161 cctgcgatga ttcgttatat ttacgggaca cgcctctttt cattgtgctc cttttcagac
     5221 ttcattttcc gtaagtgttc cccggcataa aggaggtacc tttctggcgt ttataactct
     5281 ctttatcggt gacgccggta atacagggaa taccacaccg acaaacgagg tattaacaga
     5341 cgatatttca gtgtccataa tttttctgtc cggttaaata acgtcagtct gctgccctct
     5401 gactgtaatc atcaaagccg gaatgcatac cgggttacaa cgtaaaaaaa gtgatgctgg
     5461 cccccggtgc agctggcggg cgaaacatgt ttacctttca ataccccctg tctgcagtct
     5521 ccactcttcc atcctcttaa gctacgtcgg ccacatgagt gcacttccat gataacaggt
     5581 attcctgtca gcataacgaa gccggttacc ttcccttcct cctgcatttc ctgtcagaat
     5641 gactatacag cgcgcagatt gcctttttat tgaccatctt aatataccgt tataaacttc
     5701 tttttatcat attattatca ccttattatg tttaattttt aagcagtcgg agaaagagga
     5761 tggttcagaa aattttgtcg gataaggtta tgaatgaaag aacaaatgcg tattactctt
     5821 attatcttgg agagcgtaat atctccgtac tgccccttaa tgtttatgat cccccggaac
     5881 gttttatcgc ttacataaaa aaaaacagag aaaatctgaa catcacactt tcagattttg
     5941 aacttgaaca aataatttct ggtatgcgtc tgaaggccct ggccttcctg gtccccctgg
     6001 aaaaaatatc ctggatcgcg ggcagtgaac gcgcatgctt attttcctgg tatctgttga
     6061 tgcagtttat ccagaataac agggcaaaaa taagcgcaga cttgcttcaa aagaataaac
     6121 tgtatcttaa agaagagtat ctggaaggga atgccttccc gtcggactca tcgacacagt
     6181 tcagacaaat actcagagtt ctcgatattc tttctgataa gaatttgcgg gatgagtgga
     6241 taatccagac caaagacaga tggatgcggg catttaaatc aaagagtcct ttcagttacc
     6301 tcctcccgga gaacgaacat gaatgcatat ggacatggaa ttatttgaag ggaaaaaata
     6361 ttgcactgga aaaactggcc agttttcctg gttctgctga tatttaccac gcaattcacc
     6421 tttcatttga catatgggtt acctgtccac tcacctcacc tgacgatata aaaaatttca
     6481 gaaacagttt caataaggcc aaggcgcagc gcaagtacaa aaaaatgcag gaagataaag
     6541 tcaatgtaca gttttttctt gacgcagaaa ccagggcgca gctcaaagaa ttatcgaggg
     6601 tcagaagact cagtaccggc gaaatgctgc atgacctgat tgttgaagag tataaaaggt
     6661 accgacattc cagataattt cagacatata ttatctccgt gagtcccccg ccacctttcc
     6721 ggtgtgc
//
LOCUS       DAJTPG010000162         6448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTPG010000162 DAJTPG010000000
VERSION     DAJTPG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..220
                     /locus_tag="OO230_003765"
     CDS             <1..220
                     /locus_tag="OO230_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5693353.1"
                     /translation="RQYSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKL
                     AEWVREQLNKKHSNVVACALANKLARIA"
     gene            complement(559..1329)
                     /locus_tag="OO230_003766"
     CDS             complement(559..1329)
                     /locus_tag="OO230_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693354.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1374..1808)
                     /locus_tag="OO230_003767"
                     /pseudo
     CDS             complement(1374..1808)
                     /locus_tag="OO230_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1820..2041)
                     /locus_tag="OO230_003768"
     CDS             complement(1820..2041)
                     /locus_tag="OO230_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693355.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2042..2725)
                     /locus_tag="OO230_003769"
     CDS             complement(2042..2725)
                     /locus_tag="OO230_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5693356.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2789..3100)
                     /locus_tag="OO230_003770"
     CDS             complement(2789..3100)
                     /locus_tag="OO230_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693357.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3097..3999)
                     /locus_tag="OO230_003771"
     CDS             complement(3097..3999)
                     /locus_tag="OO230_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5693358.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4439..5398
                     /locus_tag="OO230_003772"
     CDS             4439..5398
                     /locus_tag="OO230_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5693359.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5398..5793
                     /locus_tag="OO230_003773"
     CDS             5398..5793
                     /locus_tag="OO230_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5693360.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5976..6356
                     /locus_tag="OO230_003774"
     CDS             5976..6356
                     /locus_tag="OO230_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5693361.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1763 a         1674 c         1561 g         1450 t
ORIGIN      
        1 ccggcagtat agcactggag gaaaatcgac tctgttgggt atcagtaagc gaggtgataa
       61 aaatttacgc cggttactgg ttcagtgtgc ccgctcattc atgatgcagc ttgaacgcca
      121 gcatggaaaa cttgctgagt gggttcggga gcaactcaac aagaagcatt cgaatgtggt
      181 tgcctgcgct ctggcaaata aactggcccg aattgcctag gcaatcacta ctcagcaaaa
      241 agaatatcag gcctgatata tagatgctga caaaactcaa ttagcttatt gcaaacaata
      301 agatacaagt taccgtctgg ttttgcgaag attgattatt gatgacatga acggcaaatc
      361 gacctgatta aaaacctaac aggggaaaat ggctcactga agccgagggg atttttagga
      421 ttatcaggtg cggaactcat cgaggcgcgg gcatcccttg cccacagaga cgccggatag
      481 atttaagcaa gcccaccatt aatcaaaaat cagtgttgca aaaacggggg tgaccataga
      541 ttttccccgc catcgtcatc atttccggat ttttgccagg gcatccgcca tgctgcggat
      601 ttcggcccgg gcttcccgga caacttcccg cagaacacca cggtcaaacc accggcgggg
      661 cggttcatcc ggtatacgca gcacatcgca ggccagccca ctggacagga tatgctcaat
      721 acaatcgctg accagataca tttccaccgc cgaaaaacgt gcgccccggt tcaggatggc
      781 atcgagcacc acgggcaggt tttcatcgtc ttcatcgagc cagcaggggt gatcgctttc
      841 cttcacgacc aggcgaaagc ggcgcgtctg gcgggtcagc aacgtctgac cgcttgtggt
      901 cagcgccgcc cggatacgcc gcgcatcttc tgccccccgg caactgacac tgcatactgc
      961 atcgcgcacc gcatcaagaa ctgccctgat ttcgtcttcg gtcagccatt caccggcata
     1021 cgggaaagac acctgcgttc cgtccgccgg aagtaccggc agcgactgcc actgactgcc
     1081 ggacagaaca agttccgctc cggttccggt cagtttctga atggcggagg ccaggtgcgg
     1141 cggcagcacg ccatcgtggc ggttaacgag taacgtcgcc agccggggcg catccagccg
     1201 cacgtaaatg ctgatccagc ccggatagtt cgggttacat tggtcaacat ccgtctcttc
     1261 cggccagccc tgcgccagca gcaggttacg ggcgtgacga caaatcagag cattcagcgt
     1321 ttcattcata attcatgaac tccctgtatc gccgcccaca ggcggcgata acatcagtcg
     1381 ttttcgtgtg tgttgttttt cagcaggctg cgggcctgat caaccatcgc ctgtcggcgg
     1441 gtaacgtatt ccgggagggg gaaaccgtga tcccgggcag cccattcacg tttccacagt
     1501 gcctgttcag cgatctgagt cagggtctcc agcagttcag cagggacggt cacctggcgg
     1561 ttttcgcgca cgttgctgat aatctccatc gccgcggcgg aagaaatacc ggactcaatc
     1621 cgctagtctt ccgggatgtc ctccaggtgc gccaggacgg tcctgatttc atgatcggac
     1681 agggaaatat ccattccgtc agcgagggcc tgaatctctt ccggggacca gataaccagc
     1741 atcagcggcg tgtctcccga atactttgct gccagcgccc ggcatagcgt ttcgcatgtt
     1801 ccgtacatac tctctcccct caatctttga agcaggaatc cagttccaga atttcggtat
     1861 atgcctccat caagaggcgg tttgcgcgaa acaacgtctg acgcaccaga cgttccggca
     1921 gggtgtcagc attaaagctg taccgccgct ccatctcatt catggttgta cgcaggtcgc
     1981 acatggcatt ggccacctcc gccacttcgg cgcgaagttt ttcgtgtccg gcatagttca
     2041 tttacgcagc ctccggcata aaccagtcat cattcgcggc cccctgctgc atggcccgct
     2101 gtacggcagc cagtcgttgc tgtccggcac ggtgatactg ctcaagcagc tcgataccga
     2161 tataccggcg tccggactgg agggcggcga cgcaggttga accgctgcct gcaaacgggt
     2221 ccagcactat cgcattcggg tgtgtgaagc tctcaatcag cggttgcagg ctggtaacgg
     2281 gcttttccgt cgggtgatgg cggttgcccg aatatttcca gcccaacacg tccggcagcg
     2341 ggttttgtgg cagacgtgga cggccttttg ccaggatgta ggcgcattcg tggcgatagc
     2401 ccacatatgc ggccttcgat gtgtagtttt tggtgaagac caggtgacca acaacgctga
     2461 atcccgcatt tttccaggca gacataaagc gatcgacgcg gttccagcca tagaagctca
     2521 ccatcagcgc gtcttttttg agtacgcggt acatttcatt acaggccggt tgcagccatt
     2581 catcagtttt atcaccagca atggtacgcc cctgacggtc acgaaaaccg acgagatatg
     2641 gcggatcggt gaggatgaaa tcaatggcgt tatcaggaat acgggccata acatcgatgc
     2701 agttaccgag gacaaaacgg gacataacag atccttctct tacagagggc acccctgtgg
     2761 tgcccgttcc ccgctgcctg tgggctgttc atcgcttccg gtaacgggtc aggccgaccg
     2821 tggaataccg cagccgtcgt ctttttgaca gcaaggttat gccgcgaaag cggcttgaag
     2881 ggcgttcatc agaaaagggc gacgggctgg cgtacactgt caccgacacc agccgggagc
     2941 ggtaaaagcg cccgtcccgt gcccgccgga cgcgggacac tgaccacagg cagcgtttat
     3001 gccgcgccgc gtcagccggc gcagtaaaaa gaggagcacg gcgacatcct ttgtcggaaa
     3061 agaaacgggc ggttgcccgc ccgtctccgt catacttcat ccgccataat gcgccacatt
     3121 gtcgactatc cacgcaggtc cggtgacttc cggctgctca tcgtcatctg tcggggaaga
     3181 ccattcaagt tccccccaca gcacattgac gagctctcca cgctcataca actgccagcc
     3241 acagaaatca cagccctgtt cggcatacca gtgctccagc gtgcagctga aacggcggct
     3301 taattctgca ataacgtcgc tttccggctg acaccacgga gtgtcgaaat ccacctgaat
     3361 gaagttttct ccctgactgc tgatattcag gtcatatcca caaggccact tcacgccata
     3421 ccgttccgta taccagtgat atgcagaagg aacaccgttc agaacaccac cattaaagcc
     3481 gttcacttca acatccaggc gggtcggcag aaccgtcagc aggtcaaacg gcatggtttt
     3541 ttccggcact acgttgtcac acagacggtt ccaccacacc gatacatcct cgttacccca
     3601 tatgtcacac cagtcacctc tttttgcggt gaaatgcaca gtgatggttt ccctggcatc
     3661 atccggtaat ccttcccaac gacgctggcc agtaccgctc tgtaaccaga gttcatgcag
     3721 catcaggcag ttttgttcat ccagcaacac accgttctgc aggtgtgtca gccagcgggt
     3781 gaacgcgata ttttccgtcg atacagcacc acgtccggca gccgtcactc cggggcactg
     3841 ttcagagcgg atattttcag tgatctgcag aagtccggca cttccggcca gaaacagctg
     3901 aataccttca tttgtggcgc ggcgataaag cggtgtgact gcaccgctgg ccagtcgttt
     3961 aatctcagcg atctgtgccg gctcaccagg aaaatacata cgattcgagc accagtttgg
     4021 catgggaatt ctcctctcac atgcggcctc catcaggccg tgccgcacct gtaaccgggc
     4081 gaccgcatac gaatgcaggt gcggccccga agccgcagcg cgtggaggag gaatgacgaa
     4141 acgtgaccgg gtgaggaggc cggcgacagc gacacacttg catcggatgc agcccggtta
     4201 acgtgccggc acggcctggt taaatagtat ccgtctcagt tcgtggcgca aaatgagaaa
     4261 ccaataagaa ctcaaaatga accacaacaa gaacataaag agactctaaa agagcaacac
     4321 cgtttgacat agactcaaaa tgagtcacaa taagaaacaa taaaaaactc aaaaagatgc
     4381 accgacataa aggatgctca atttgagata aacacaaact caaaacgagg caaaaagaat
     4441 gctaaaagta tcatgcgatg atggttcaac taacgtaaaa ctggcctggc ttgaagatgg
     4501 cgaggtaaga accagtctgt caggaaacag ctttaaagaa ggctggaacc cgggactgtt
     4561 taatgccggc aaagtttaca attacgtcgt ggatgaaaaa aaatacacct atgatttggg
     4621 aagcactgct gtcataggta caacacatgt cagttatcaa tacagcacca ccaatctgct
     4681 tgccatacat catgcactgc tgacatccgg ccttcagcca caggatgtag aactgacagt
     4741 aacattacct gtgaccgaat tctttgataa tgacaatcaa ccaaatgaag aaagaataga
     4801 aagaaaaaag gcaaatgttc tccgtgagat atctctgaat aaaggggaaa catttaaaat
     4861 taaaaaagtt aatgtaatgc ctgaatcttt gccagctgca tttgaatcac taaaaaaaga
     4921 taaagtaaac aaacttgaac gcagcctgat tatcgatctt ggaggaacca ctctggattg
     4981 tggtttaatt ctcggtgcat ttgaaggcat ttctgaaatc cgtggctatt cagaaatcgg
     5041 gacatcccgc atcactcata ccgtcatgaa tgcgctgacg aaagcctcca caccatgtaa
     5101 ttattttatt gctgatgagt taataaaaaa cagacacgat aatgaatatc tccagacatt
     5161 aataaatgat gtagctgaaa taaaaaacat ttctcatgta atagacagag aagtgaaatc
     5221 tctggcagaa agcatcaggc aggaaatttc aacattctca ggaatgaacc gaatctatct
     5281 cactggcgga ggagccgagc ttatttaccc tcacataaag caatatttcc cgaatctgaa
     5341 agtgaacaag gtggatgaac ctcagtttgc attggtgaaa gcaatggtgc atgcgtaatg
     5401 gaaagcagtg atccaaaaaa aagaaagaaa gttgtggcat acctgcaccc ggcactttat
     5461 ccgcaggaca acctgacaca acaaacaata gactctctcc cagtacaaat gcggggagat
     5521 ttctacagac aatcccttat ttgcggagca gctctttatt ctgttgcccc ccgactgtta
     5581 acgctaatca gcgttttttt cagtgagaaa atcactgcag aaaatctggt gaaacttata
     5641 gaacaaacaa caggatacac atccacatca attgatataa gtgtgttaaa aaacatcata
     5701 gaggcatctt cagaaaacaa atcagagagc atcacttcaa aagatgattt cgaagaacaa
     5761 accagacgca atctttctat gttaaaaaaa taataaaaga agagcaggtt cagtcaatta
     5821 cctgctcttc ttttatacat actaaaaaat cagattttta cgaatattca gattatcata
     5881 aaaaatgcaa cagacaggaa tgcaataatt ttgcattatc tcgttgcttg taatgcaaat
     5941 atgcattatt gtcagtgtgt gacaataaca gagagatgac gatggcgacc attaacgcac
     6001 gtattgatga tgatataaag aaccaagcag atgaagtgct gaagctgatg aatatcagtc
     6061 aaactcaggc tattgcagct ttttatcagt atattactga acaaaagaaa ctgccatttg
     6121 tgatcacctc aattgtaaaa acgccgcatg atttattacg tgaaagtaca gacatgttag
     6181 ctgaagctct tgcagtgatt tccaatctac aggtgtggac tgaacagcag gatgggattg
     6241 gaaaagcaaa gctaatggag tattaccgcc gcctcgacgc actgtactgt tgtgcaaaag
     6301 agaaaatagg tcttctttca gataaccggg atgctgaact aggctgtgtc ccttaatcat
     6361 ctgagttata gtaactgtct gtttacacaa catattgaac tatggctcgc tacgaccttc
     6421 ccgatgaggc atggaccatc atcaagcc
//
LOCUS       DAJTPG010000163         6260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTPG010000163 DAJTPG010000000
VERSION     DAJTPG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..2137
                     /locus_tag="OO230_003775"
     CDS             <1..2137
                     /locus_tag="OO230_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5693362.1"
                     /translation="VPFVFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKF
                     CLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTG
                     KTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAY
                     EIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSAT
                     QIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPT
                     KGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDI
                     DHAERMRRALVNLNPQQVKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSEL
                     MTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFAD
                     ERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDD
                     VGPFPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASL
                     DDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKE
                     RAENVHKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEII
                     KTGFGDRNGYNQALSELENEIYQLPPRSA"
     gene            2204..3673
                     /locus_tag="OO230_003776"
     CDS             2204..3673
                     /locus_tag="OO230_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5693363.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            3673..5334
                     /locus_tag="OO230_003777"
     CDS             3673..5334
                     /locus_tag="OO230_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5693364.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5555..5896
                     /gene="symE"
                     /locus_tag="OO230_003778"
     CDS             5555..5896
                     /gene="symE"
                     /locus_tag="OO230_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5693365.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(5943..>6260)
                     /locus_tag="OO230_003779"
     CDS             complement(5943..>6260)
                     /locus_tag="OO230_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693366.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1834 a         1494 c         1439 g         1493 t
ORIGIN      
        1 cgttcctttt gtttttgcca ccaacggtga tggctttatc ttccgcgatg ccaccgcagc
       61 cgaaggtgaa tgccttgaaa agcaaataac gctggatgac ttcccctccc ccgctgaact
      121 ctggcaaaaa ttctgcctct ggaaaggata cacacaagct cagcttccgg tgattactca
      181 ggactattac gacgatggca gcggcaaatc gcctcgttat tatcaacttc aggcaatcaa
      241 caaaaccatt gaagccgtct ccaacgggca aaaccgcgtt ctgctggtca tggcgaccgg
      301 aacagggaaa acctataccg cattccagat catctggcgc ctgtggaaat caaaaaataa
      361 aaaacgcatt ttgttccttg ccgatcgcaa cattctggtc gaccaaacca aaaataatga
      421 tttccagcca tttggtacgg caatgaccaa agtcagcgga cgcaccattg atcccgctta
      481 tgaaattcac ctcgcgctct atcaggctat aactggcccg gaggaagacc aaaaagcgtt
      541 taaacaagtc gcaccagatt tcttcgatct gatcgtgatc gacgaatgcc atcgcggcag
      601 cgcatccgaa gacagcgcct ggcgagagat ccttgattat ttcagttccg ccacccaaat
      661 tggcttaacc gccacgccaa aagagacgca tgaagtctcc agcacggatt acttcggcga
      721 tccggtttac gtctactcac taaaagaagg gatcgaagac ggcttcctcg ctccttataa
      781 agttgtccgt gttgatattg atgtcgacct gcaaggctgg cgcccaacca aagggcaaac
      841 tgacttaaac ggcgaagtga ttgacgatcg tatctataac cagaaagatt tcgatcgcac
      901 gatggtgatc gacgaacgca cagaactggt tgccagaacc attaccgact acctcaagcg
      961 caccaatccg atggataaaa ccatcgtctt ctgtaacgac atcgatcatg cagaacgtat
     1021 gcgccgcgcc ctggttaatc tcaacccgca gcaggtgaaa aagaacgaca aatacgtcat
     1081 gaaaatcacc ggcgatgatg aaattggcaa agctcagttg gataacttca tcaacccgaa
     1141 aaaaccgtac ccggttatcg cgaccacttc agagctgatg accactggtg tggatgctaa
     1201 aacctgcaaa ctggtagtac tagaccagaa catccagtcg atgaccaaat tcaagcaaat
     1261 tatcggtcgt ggtacacgca tcgacgaacg ttacggcaaa ctctggttta ccatcctcga
     1321 ctttaaaaaa gccaccgaac tgtttgccga tgagcgtttc gatggcattc ccgaaaaagt
     1381 catggatacc acaccagagg atatcgccga tccagaatct gattttgaag agaaactcga
     1441 agaaatcagc gaacatgacg acgaacaggt aacaggcgtt gatgaaccgc ctgcgccacc
     1501 ataccaggtt accgataccg atgatgtcgg cccatttccg gaagaagacg agaagaaaat
     1561 ccgcaagttt cacgtcaacg gtgtagcggt gggcgttatt gcacagcgcg ttcagtatta
     1621 cgacgccgac ggtaaactgg ttaccgaatc ctttaaagat tacacccgca aaacactgct
     1681 caaagaatat gcctcgctgg atgactttac ccgcaagtgg caggacgccg atcgcaaaga
     1741 agcgatcatt cacgagctgg agcaacaggg gatcatctgg gaagtactgg cagaagaagt
     1801 cggtaaagat ctcgacccgt tcgacatgct ttgccacgta gtgtatggtc agccgccgtt
     1861 aacccgcaaa gagcgcgccg agaacgtgca caagcggaac tacttcacaa aatactctga
     1921 agcagcgcaa gccgtgctcg ataatctgct ggataaatac gccgatgcgg gcgtacagga
     1981 gatcgaaagt attcaggtgc tgaaacttaa accattcgac agcatgggca ccttaccgga
     2041 gattattaaa accggatttg gcgaccgtaa cgggtataat caggcgctca gcgagctgga
     2101 aaacgaaatc taccaattac cgccccgctc tgcttaacaa ctctcttttg cgccttctgc
     2161 aaccatcatg ttgcagaagg ccttgttttt catggaatac aacatgtcta tcagctcagt
     2221 aatcaaatca ttacaagata ttatgcgcaa agatgccggt gtggacggcg atgcgcagcg
     2281 tctcggtcag ctctcctggc tgctgttttt gaaaatcttc gatgcccagg aagaggcgct
     2341 ggaactggag caggataact atcaatatcc gatcccacag cgttatttgt ggcgcagttg
     2401 ggccgccaac gcgcagggca ttaccggtga ttccctgctg gaattcgtta atgatgatct
     2461 gtttccggcg ttgaaaaacc tcactgcgcc tatcgataaa aacccacgcg gctacgtggt
     2521 gaagcaggcg ttcagcgatg cctataacta tatgaaaaac ggtacgctac tgcgccaggt
     2581 gatcaacaag ctgaacgaaa ttgactttac cagcgccagc gaacgccatc tgtttggtga
     2641 tatttacgaa cagatcctta aagatctgca atctgcgggc aatgcgggcg aattctatac
     2701 tccacgcgcc gtcactcgct ttatggtgga tcgcgttgat ccgaaactcg gcgaatccat
     2761 tatggacccg gcctgcggta cgggcggttt tcttgcctgc gcgttcgatc atgtaaagaa
     2821 caaatacgtg aagagcgtcg ccgatcatca gacactgcaa caacagatcc acggtgttga
     2881 gaaaaaacag cttccgcacc tgctggcgac caccaatatg ctgctgcacg gcattgaagt
     2941 accggtacaa attcgtcacg acaacaccct gaataaaccg ctttcctcct gggatgagca
     3001 actggatgtc attgttacca acccgccgtt tggtggcacg gaagaagacg gtattgagaa
     3061 gaactttccg gcagagatgc aaacccgcga aacggcggat ctgtttctgc aactgattgt
     3121 ggaagtactg gcgaaaaacg gtcgtgcggc ggtggtattg ccggatggca cactatttgg
     3181 cgaaggtgtt aaaaccaaaa tcaaaaagct acttaccgaa gagtgcaacc tgcataccat
     3241 cgtgcgttta ccgaatggtg tgtttaatcc ctataccggc attaaaacca acctgttgtt
     3301 ctttaccaaa ggtcagccaa ccaaagagat ttggttctat gaacatccgt atccggcggg
     3361 cgtgaaaaac tacagcaaaa ccaagccgat gaagtttgaa gagtttcagg cggagatcga
     3421 ctggtggggt aacgaggccg atggttttgc cagccgcgtc gagaatgagc aagcgtggaa
     3481 agtcagcatt gatgacgtga ttgcgcgcaa cttcaatctg gatattaaaa acccacatca
     3541 ggcggaaacc gtcagccatg atccggacga actgttagcg cagtatgcaa aacagcaggc
     3601 ggagatccag acgctgcgta atcaactgcg cgatattctt ggcgctgcgc tgtctggcaa
     3661 ggaggttaac taatgagtgt ggaaaagctg atcgttgatc atatggaaac ctggacctcg
     3721 gcgttgcaaa cccgttccac cgccgggcgc ggcagctccg gtaagattga tttgtatggt
     3781 attaagaaat tgcgtgagct gattctggaa ctggcggtgc gcggtaaact ggtgccgcag
     3841 gatccgaatg atgaaccggc gtcggacctg ctgaagcgta ttgcggcgga aaaagcagag
     3901 ctggtgaagc aaggcaaaat taaaaagcaa aaaccactgc cagaaattag tgaggaagag
     3961 aagccgtttg aattgccgga gggatgggag tgggtgcacc tccctgatat ttattgctca
     4021 atttctgagt catctagaaa aataaaatcc tccgaaatac tacctgaggg aaaatatcct
     4081 gttatcgagc aaagccaaga atttatttct ggttattgca acaatgaatg tctgttaata
     4141 aagttaaata atccagttat agtttttggt gatcacacaa ggaatattaa atttattgat
     4201 tttgattttg ttgtcggcgc tgacggtgtg aagattttat cacctatatt aatttgtgag
     4261 cgtttctttt tttggcagtt acgatcattt aaattagatg taagaggata cgcgagacat
     4321 tttaaagtat taaatagctg cttatttgca ttacccccca ttgcagaaca agagcgaata
     4381 gttgaaaaag ttagctcttt gatgtccctc tgcgaccaac tagaacagca atccctgacc
     4441 agtctggacg cacatcagca actggttgaa accctgttgg gaacgcttac agacagccaa
     4501 aacaccgcgg aactggctga aaactgggcg cgtattagcg agcatttcga caccctattt
     4561 accaccgaag ccagcgtgga tgcgttaaaa cagaccattc tgcaactggc cgtaatgggc
     4621 aaacttgtgc cgcaggatcc gaatgacgaa cccgcctctg aactgctcaa acgtattgcg
     4681 caggaaaaag ctcaattggt gaaagaaggg aaaataaaaa aacaaaaacc actgccgcca
     4741 attagcgatg aggaaaaacc gtttgaactt ccggaagggt gggaatggtg tcgtattggt
     4801 aatatagtta atattaaatc tgaattagta tcaccaaaag attatttaaa tttatatcaa
     4861 gtagcacctg atattattga aaagggcact gggagagtga tatcaaagag aacagtgaaa
     4921 gaatcaggag tcaaagggcc aaatagtcgc ttttataaag gacaaattgt ttattctaaa
     4981 atccgcccgt cattatccaa agttttttta gcagagtata atggtctttg cagtgctgat
     5041 atgtatccat tagactgtta cattaatcca aattacttat tgaaatatat cctttccatt
     5101 ccatttctta tgcaagtcaa aaaagcagag aacagaataa aaatgccaaa acttaattct
     5161 gacagttttt acaatattat tgttgcgatt cccccctata atgaacaaca agcaatattt
     5221 gataagataa attctattga agcagtatgc aatggtctta tttcttatat tgggatttat
     5281 cataaaaccc aactgcatct tgcagacgca ctcactgacg cagcgataaa ctaattccct
     5341 cgccctcaca aaaccaataa agcggctaaa tagccgcttt attcacatca ccaaaattta
     5401 tatttcacgc taattttcat cttattttca ttaattttta gctggacttt ccctgtattt
     5461 actgatgatg tatccaggta tttagcacgg tgcggatgtg cgccaacaca ctcgcaccgc
     5521 taatcacaat cactatttat agagaatagt aatcatgact gacacgcatt ctattgcaca
     5581 accgttagaa gcagaagtct ccccggcaaa taatcgtcaa ttaaccgtga gttatgcgag
     5641 tcgttacccg gattacagcc gtattcccgc catcaccctg aaaggtcagt ggctggaagc
     5701 cgccggtttt gccaccggca cggcggtaga tgtcaaagtg atggaaggct gtattgtcct
     5761 caccgcccaa cccgccgccg ccgaagagag cgaactgatg cagtcactac gcaaggtgtg
     5821 taagctgtcg gcgcgtaaac aaaggcaggt acaggaattt attggggtaa ttacaggtaa
     5881 acagaaagtc gcctgataca ttgacccggt agcgccagtg ctaccgggta ttattcgccg
     5941 cgttatacca acgcgctctc catctgtttt tcaaatgcct gacgaattaa tcccataatg
     6001 taaggaagtt gtgcgaggtc gctgaaacca atttctacat cgccattacc ccagcgccca
     6061 acatttgtca catctttggc aatacctttc ggatcgacca gttcatgaaa ctgcatatta
     6121 agcgacaatc gcagtcggct tttttgcggc acaacatcaa caaaattcgt ttccgcttta
     6181 aacgcaatat atagcttcaa tacttcctgc gtgatccctg cgtccaggcg cataacttca
     6241 tcgcgaagat gatcgaataa
//
LOCUS       DAJTPG010000164         6256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTPG010000164 DAJTPG010000000
VERSION     DAJTPG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO230_003780"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO230_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5693367.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO230_003781"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO230_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5693368.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO230_003782"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO230_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OO230_003783"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OO230_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1659 a         1458 c         1477 g         1662 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgatg
//
LOCUS       DAJTPG010000165         6151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTPG010000165 DAJTPG010000000
VERSION     DAJTPG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..171
                     /locus_tag="OO230_003784"
     CDS             <1..171
                     /locus_tag="OO230_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5693369.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO230_003785"
                     /gene_synonym="chbF"
                     /pseudo
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO230_003785"
                     /gene_synonym="chbF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO230_003786"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO230_003786"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5693370.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO230_003787"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO230_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5693371.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO230_003788"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO230_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5693372.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6151
                     /locus_tag="OO230_003789"
                     /pseudo
     CDS             5430..6151
                     /locus_tag="OO230_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1576 a         1309 c         1557 g         1709 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcatg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 agaattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac g
//
LOCUS       DAJTPG010000166         6060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTPG010000166 DAJTPG010000000
VERSION     DAJTPG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..145)
                     /locus_tag="OO230_003790"
     CDS             complement(<1..145)
                     /locus_tag="OO230_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5693373.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQM"
     gene            381..653
                     /locus_tag="OO230_003791"
     CDS             381..653
                     /locus_tag="OO230_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5693374.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(619..1026)
                     /gene="nudG"
                     /locus_tag="OO230_003792"
     CDS             complement(619..1026)
                     /gene="nudG"
                     /locus_tag="OO230_003792"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5693375.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALLRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1113..1733
                     /locus_tag="OO230_003793"
     CDS             1113..1733
                     /locus_tag="OO230_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5693376.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1742..3049)
                     /gene="ynjE"
                     /locus_tag="OO230_003794"
                     /pseudo
     CDS             complement(1742..3049)
                     /gene="ynjE"
                     /locus_tag="OO230_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3116..3769)
                     /locus_tag="OO230_003795"
     CDS             complement(3116..3769)
                     /locus_tag="OO230_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5693377.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3769..5240)
                     /locus_tag="OO230_003796"
                     /pseudo
     CDS             complement(3769..5240)
                     /locus_tag="OO230_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5213..6060)
                     /locus_tag="OO230_003797"
                     /pseudo
     CDS             complement(5213..6060)
                     /locus_tag="OO230_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1428 a         1621 c         1600 g         1411 t
ORIGIN      
        1 acatctggcg atattttgga ttttgttcaa gaaaaggcca gagtgtggtc attacttcac
       61 gaacggcttg cgcgaactcg gtttgattcg ggtcgcgctt ttggacatgg ttgaggaatg
      121 actccagaga atatgtctga tccatagata taaaaacctt atatattaat acgattgtgc
      181 ttatgttgtt tatgtcatgt gcttttgcag ttttcgacta taccatcaag aatgtgacgg
      241 taagcaagaa aaaaatgacc caggaaagca aattaataac gagagtaatc tcataaccaa
      301 atgcgataac tcagacgcta aagttttgcc acccggctac cgttatcagt agcagagaca
      361 ataacgttaa ggagttccct gtgagtcgag cattgttcgc cgtagtatta gcatttccat
      421 taattgcact ggctaacccg cattatcgtc cggacgtgga ggtaaacgta ccgccagaag
      481 tgttcagttc aggcgggcag tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc
      541 aaaattattc tgaaggggcg gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg
      601 atgacaaaac ccttagcact aatccgctgc tctggcggcg cgtaaagcca taaacgcctc
      661 taataatgga atgtcagcag gggccagcgg atattgcagc gcctcttcag gtgagcacca
      721 gaccagcgcc tgatgttcat gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc
      781 atgaagatgg ataatccggc ccgaaacttc tcgctgatgg ctggcaacat actcacccac
      841 agttgcttcg atgtccaatt cttcgtttaa ctcacgcaac agcgcctgct gctggctttc
      901 atccagctcg actttacctc cggcaaactc ccataatccc gcctgatcgc tctgggcggg
      961 gcgttgcgcg agtaaaattt tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat
     1021 tttcataaat tacacacggg cagataagcg aaaaatgcca taatatccgc actgttgctc
     1081 aatcagaaag gctttatatt taaggagtgc ctgtgctaga ccgccatctt catccccgga
     1141 ttaaaccgtt gttgcatcag tgcgtgcggg ttcttgataa accgggcatt acgccagatg
     1201 gtttaacatt agtgggattt gccatcggcg tgctggctct gccgtttctg gcgctgggct
     1261 ggtatctggc ggcgctagtc gtcattttgt tgaacaggct gcttgatggt ctggacggtg
     1321 cgctggcgcg gcgcagaggg cttaccgacg cgggcggctt tcttgatatt tctctcgatt
     1381 ttctctttta cgcgctggtg ccgtttggct ttattcttgc tgcaccggag caaaatgcgc
     1441 tggcaggcgg ctggttgttg tttgcgttta tcggcacggg cagcagtttt ctcgcctttg
     1501 ctgcactggc agcaaaacat cagattgata accccggtta tgcacataag tcgttttact
     1561 atctgggggg attaaccgaa ggcacagaaa cgatcttact gtttgtgctg ggatgtttat
     1621 tccctgcatg gtttgcgtgg tttgcgtgga tcttcggtgc gttgtgctgg atgacgacct
     1681 ttacgcgggt gtggagtggg tatctgacgc tgaagtcgct ccagcgtcag taattgcaat
     1741 gttatttgct actgcccggg ccgcgttcac cggttgcaac cggatttttt ggatcgctgc
     1801 tccattcgta ccagccgccg tcatacacgg aaacattctt ccaacccatg gcgcgtgcgt
     1861 acataaaggt ttcggacgcg cgccagccgg tgccgcagta gaatgaaact tgctgatctg
     1921 gtttgatatt ccatgctttc cacatagcgg taatatcatc ggcgctacgc atggtgccat
     1981 ccgggttata gaaatcttcc atatgcgtcg agtcgctacc agcgtgtccc caacgtgctc
     2041 cggctatttc accttttggt ttaatatagc tgtaaccgct ggtcgtaccg ataaattctg
     2101 gccacgaacg aatgctcacc agcgatgcat cctggcgatg cagcagtcca cgggcctgtt
     2161 ccatatcgag catcaactgc ggttgtgccg ggatcttcac gccgaaatcc ggttccgctt
     2221 tcactttcgg tggcgttcct cgctcaacag gcagtcccgc gtcggaccag gtttgccagc
     2281 cgccatccag cagacgcaca tctttcacgc ctgcgtagag cataatttgc gccacacgcg
     2341 ctccagcgta tacgtcacgc ccgtacagaa tgaccgtggt gtcatggcga atgccgtgtt
     2401 ttgccagcat cgcttttagt tgttcatcag aaactttgtt ccacagcggt tcgctttcca
     2461 cttcgttggt atcgatgtag tcagcgccag gaatatggct gataaggtaa agcttaggag
     2521 cgccctaggc cgcttcaatg actttccagt caccgacagg tttcgccgta acctctttac
     2581 cttgttgcag gtcatgcagc cattgcggat aaaccagctg ctcaaagtgc ggcagttttt
     2641 gtagacggga aggttcgctt agcgcgtcac tcagaatgga gatatgcgta aaacctgctt
     2701 tttgcagtcg cgttttgacg gcgtcaacat ctttgccatt accgtacagc gccaccgggg
     2761 catcggcttt caggttatgt tgcttgatcc acgcgttgag ctgttcggtg ctcattttgt
     2821 ccagccagct agcagagagg tttaaggcgg caggttcatg accagaaggg ccatttaagg
     2881 tttgtggcca gccgttataa aacgcgctgg ggcgagtatc tatcgctttg ccattttgtt
     2941 gctgaagttg gtcaagtgta agaggcttcg ccagttcagc ggcccacgaa gaagcgcaag
     3001 ccagccctaa agccattgcc agcgcggtca tttgagaaac acgtttcatc gaaaatcccg
     3061 acattgtagt tttgagtgcg ccattctgaa ctgacgcatt gagaaaaact ttgcgttatc
     3121 gcagtttgtt gtaattttct gaccactgcg ccatatccag aacagaacta tcagcaggaa
     3181 catcctggag atcgtgcgtt acctgaacga cggggatcgc cagggcgcga acttcgctga
     3241 acacccactg gcgaaaatta tcgcgcagag ccacatcaag acggctgaat ggctcatcca
     3301 ggagtaacgc ttttggctgg gcgagaaggg cgcgtagtag agcaacgcgc gctcgctgac
     3361 cgccagacaa agtggcagga tcctgatgga aagctccgtc aaggcctgaa cgctcaagtg
     3421 catcattcac ggcattacgt cgggcattcc ccttaagtgt cgccggtagc gccagcagta
     3481 aattttgccc gacgctgaac tggtcaaata acagtgcatc ctgaaaaaga atgccaatct
     3541 gacgctgtgc ggtgggtagc atgtcaatcc gttgctcatt gagccatagc tcacctgtac
     3601 aagaaaactg tccggccagt gcaccaatca tccatgaaaa cagagtggat tttccacagc
     3661 cagacggccc cattaacgtg acgatgtcac ctttatcaac cgtaaagtta acgtttgtca
     3721 gcaagcggct ttctggtaaa cgtagcgaaa catttttcac gcagagcatt agcggattcc
     3781 ttgtctgaca taacctaccc attttgcgac taacgcggtc agggcaaaaa taataagcgg
     3841 taacaacagt tgccataacg cctgggcggc gagaataccg ttgctgccgc cgctgcttaa
     3901 tgccaccgcc tcagtggtga gcgtcggaaa acgccccgcg cccagccaca gcgtcggcat
     3961 atactgcgca atactgactg aaaatcccac cgcgaaggca atcagcacag ggcgcaacat
     4021 gagtgggcat ttcacgtaaa agaagatttt ggcccgcgac cagcccagtg tttgcgcaat
     4081 caaaattaac cgtgaatcaa tgcgctgcca ggcgggttgc aggataaaca gcatccacgg
     4141 cattacccac agcagatgcc cccagaccac cgccgtccag cttccatcca ggttcagcca
     4201 tagcgccagc gtgtactggc ccgccaccag tggcagagcg ggcaatagaa tgggcaacca
     4261 tagccacaac tggcgacgct gtgcccccca ttccagccac agcagtaaca ggagcaaagc
     4321 gatgaatgtt gctaccagcc ccattgtcag actgttgatg agtgcttcac tattgatcgt
     4381 cgactgatcc gcgaggatcg ccagcagaac cacacacagc acaccggtta agggtaaaaa
     4441 actcgccagc gtagtgcctg gtaataaagg tgtggcaggc ttgcgaatgc catctacgcg
     4501 gggaatagtg cgccgccagc tgcgccaaaa cgtaggcggc gagtaacagc atcagcagca
     4561 gactggcgag tgcgcctttc gtttgttgat ccgcgtcgcc ctgagttaac cactgccagc
     4621 taattactgc cagcgtcggt ggattacctg gcccaagaat aatcgccaca tccacgaccg
     4681 aaagcgacca ggcaacaatc gccagcatcg ccattgccag cggctgtagc ccagcgaatc
     4741 cagcacaatg acctgctgca acagccgttt ttcgctcaat actgccacta agatccacag
     4801 cagaaatgcg ctttctttca ctgcaagggt gaggcccaga ccgatccaaa tcggtccatt
     4861 ggcggagtga aatacgggaa atagtcatat agcagccctc cgtcagcaaa gagcagcagg
     4921 gcgctggtgg caaaagccac atggggaatg gcgagcagcc acggcagacg ggcgcacata
     4981 cgctgccatt tcggcccagg ccatagcgcc acaatcacca gcagggcaat caacaatgcc
     5041 ccgaccgccg cgatggttgt cgacaccagc gttgccagta atgcctgcgg taactgtgga
     5101 tcggcaaata acgcctgcca gcgtgtcaac gacaacgcag gcgatatcag cgtgagagct
     5161 gccgggatca gcggtgcata aatcaccgcc accatcgccc acagcaaaaa gattaatgcg
     5221 taccgtaacg gcgtaaccat tcttgttcca gtgcattcac ccatcctgcg tgcggttcag
     5281 ccagtaccgg cggcagatcc tgcggcattc ttgattgcaa tatttcgcgc tgcccgtcag
     5341 gcagtttttg cggatcgaga acagaaggat cgccccagac aacgggatct gctttacgca
     5401 gttgcgcatc gggtgagagc aggaaattgg caactacctt cgccgcagca ctggcattcg
     5461 cgttggctgg aatggtgacg aaatgcacgt tgccaatcat cccctcgcga aagccaaaac
     5521 tgtaactgct tgccggcaaa tcgccgctgg cgattttttg ctgcgcatgt gcggggttaa
     5581 aggtcagcga caggcgcaat gtgccggctt tcagcagagc atccatccgc gcgggcgaag
     5641 gcgggaaatc ctttccttcg cgccacaaat acggatgcgg tgcatcaaga tattgctaca
     5701 agggcgcagt gacacgggcg aaagtcgcat cgtccggcgc ttcttttaat gcggcgggat
     5761 ctggcgtcag cataatcagc aactgttcaa gaaacgccgt gccggtaaag tccggtgggc
     5821 gcggataagt aaccgtgccg ggattggctt tagcaaactc cagtaaggct tgcggcgttt
     5881 gtggtggctg tggcgtaaca tcgcggcggg cgataaacgt cagttgtgcg ccgccccagg
     5941 gcgattccgc accttgtgtc ggcactgaaa aatctccccg caccggcagc tgtgtgtcga
     6001 catagcgcca gttgggcaga gtctccgccc agcccgtttg taataaattg gcctctttta
//
LOCUS       DAJTPG010000167         5997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTPG010000167 DAJTPG010000000
VERSION     DAJTPG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(1..407)
                     /locus_tag="OO230_003798"
                     /pseudo
     CDS             complement(1..407)
                     /locus_tag="OO230_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(593..3901)
                     /gene="icsA"
                     /locus_tag="OO230_003799"
                     /gene_synonym="virG"
     CDS             complement(593..3901)
                     /gene="icsA"
                     /locus_tag="OO230_003799"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5693378.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4430..5632
                     /gene="virA"
                     /locus_tag="OO230_003800"
     CDS             4430..5632
                     /gene="virA"
                     /locus_tag="OO230_003800"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5693379.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5811..>5997
                     /locus_tag="OO230_003801"
     CDS             5811..>5997
                     /locus_tag="OO230_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5693380.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1841 a         1358 c         1025 g         1773 t
ORIGIN      
        1 cggggcggtt cagttcaggc tgtacgcttc cccatgtgat ttgcctcgcc acttcaccgg
       61 gctcaggacc ccgtaggatc ttcaccgaaa aaaatgcatc accagttgct ttattatttg
      121 ttttaggtag acctgttact aatttatacc tactctgacc aagtctggat aagaaaggca
      181 cacctgcaca tttctacttg gaaatgtttt cttttaccat tttgctcaaa aatttcaaca
      241 tccaatattc ctcgtacaga actactcaag tcctgaatag aaaaagttcc agctggtaca
      301 gtagagtcat atattactcg accttgttga gatacaacaa cacgtgcatt agtttctgca
      361 ataccagtaa tctgaggcgc ataaccacga atatttggcg gtagcattcg ttcatcgctt
      421 tctaatgcaa ttcctgtgta acgccaagca acagcgcatt ctggcgcatg atctggtaaa
      481 cacgtttggc attgatcgca ggcataccat catgtgcaca aaaacccacc gagcttttac
      541 acggtgggtc ccagagaaat gcaggacatc aacacgccct gcatttttat tatcagaagg
      601 tatatttcac acccaaaata ccttgggtgt ctctgtaact gttattaccc aattgctgag
      661 atacgtttcc ccagatgctt aggttatctg caagacgccc ccccacacca gttttgagtt
      721 caatcacatt acggttgcct atctggtgat ttgacatgcc attcataatt acaccatatt
      781 gctttgagct ccatttccaa ttcacttcca aaaatggttc gaacttacga agagtctcct
      841 tatcgataca actctgacta attaaggatg tacgcaatcc caattttgct tgaatattat
      901 tgcccccaga ggatgtgacc atagtgccat tgtgttcaat atagtcgttt gcttttaccc
      961 catgcctagt tatctggact tgtggattga gatacaatgc gttgtcaata ttattatgag
     1021 cagtccagcg tatggttggg tagatatagc cagcttccaa agctcctgtt attcctttgc
     1081 tggaatattt ctcaccagtt aacccatctc ctttgactgt attattaaac caactgtact
     1141 gcaaccaagc atccatatat agacctgttc gttctttttc atcctgaaac caagaactgt
     1201 acaaaccggc agtataaccg ctcacagttc ctcgtgaact ataactagta tacttattat
     1261 gagttttgct atcctgatat ccataagctc ccataatacc cacatgcaag ccatcatgat
     1321 cagtgaagtt tgtcttcaaa atatctccac caatctgata aaccatcctg ttagctgtgg
     1381 tttttaattg tctatcagct aaattatgct cattatgtcc accaacagta cgaatccaca
     1441 tggtggtttc gctgagctga cctgtagtag gatcaacaat agtgctaccg tgtcggtcat
     1501 agagagaagg actaaacatc gtgttcattg ctatcaggtt agcaatataa ctgccagctt
     1561 cagggcgcca tgtgtgaata ctaccttcac ccaaattaag tgaagataca agctgtgaac
     1621 tagctcggcg actactcatt tgagtagact cttgattgct catctgtttt gattcttgat
     1681 tatccatctg actagttaga taccacttat tggtattcag tccagataca gtgccctgtt
     1741 tcaggcgata gtcataacta ccagcaacaa tacggccttt ctgaataaaa gcattcttat
     1801 cagaagaatc tgtcgaaata atttgaacac cttcaagagt tttatctcca gagcctccct
     1861 catttgtcac tgcaacatag gtaataccag acgcatggcc tgcaatttcc agccggtcag
     1921 ttttactgtt gtcattgccg agaaccgcgc ccaaagataa tattccgccc tttccatgcc
     1981 aattaccttt ctgaacatag gtctgaccga catttgacga actattattg agaataacat
     2041 gaccgttttt ctccagagtc atgttacctg tgacaatgac ggggtcaggc aaaaaagggg
     2101 cattaatatt attgattagc acagtcccac tatttaataa agtgttaaca gtttggttca
     2161 tgcctgaaaa attcaaggta gcacctttat taacaataac accagcggta cgtgtcatag
     2221 cttcaactgt ccccattttt agaatgcctg cattgatgtt ggtgaaacca gagtaggtat
     2281 ttttttccgc caaaatgaga gttccctccc ctgattttgt tagggatttt ccgtcccagc
     2341 tatttatatt atgatggctg agattatctg ccagaatagt actaacagta agttcagcgt
     2401 tttctttcag attgaactct ccatgactgt cgccatctgt atcattccag ctcaaaccgt
     2461 agatcaattt attctggtca ctgatatagc tttttgtata gtcaatagca ccagtagtga
     2521 gtaacggatt gatgatagat atattagaaa cactgaaacc tgttatccct ttatctgcac
     2581 ttagcagttt atagtccttc tgcgcccagt tatttataat agcctccact tttacattgt
     2641 tgccaaatga catatttcca gaagcttcca gactactgat tccagctaaa tttaaagaac
     2701 tgttctcacc aaaagaaacg tcacctgaaa cagtgaattt gttacctgat agtgaaacag
     2761 ttgtacaatc accagcacat aaatcacctt caatagttgg tgtggtccca gtgtaatcat
     2821 tgttgataat tttcagaatg gatgaattgt taacctgtac atcaccagta atgacagaac
     2881 cttcatggag tattaatgaa ttgtttccac cagtgatgtg gatagcatta cctgctacat
     2941 tttgtgttcc atcatcttct ttacctgaaa ttatatatcc gctattagca atggtcatat
     3001 tgctaccatt tatagcatcc ccccaatgtc caccattacc gcctaaaata gctccattgt
     3061 tgattacaga caggttatct cctgtgatag cattaccacc gtagccatca taaccataac
     3121 cgtaagcagc acctccgttg cctccagcga acgtgccctt attgattaca gagaggctgc
     3181 ttcctgtgat ggcatcacct ccattaccgc catcaccctc accataattg ttacctccat
     3241 taccaccgga aatagtccct ttgttgatta tgggtaaatt gactcctgtg acggcatcac
     3301 ctccattccc cccatcacta tcactataac tatcgccacc atgtccacct gaaataattt
     3361 ctccatttat tataaacaga ttgtctcctg taacagcctc accaccatca ccgttatggt
     3421 cagcaccgct accgccgcta ccaccaagaa tcatgccttg attgattata gacaagtcag
     3481 atccggtaat agagtcacca ccattaccgc cacaactatt accatcatta ttatcaccag
     3541 caccaccatg acctgcgcta atcataatat cagagttaat ccgtagctta gttctagatg
     3601 catgagaggg gttggaagaa gttatatacc acgcatccat tccatcttca ccagctccca
     3661 agggagaaag tccatcaaca ggtgttaata atttttcata attgtcctct gaaaaatgaa
     3721 gttcttgagt acccgaaaga ggagtagcaa aagctattgg ccccccgagc aacagggatg
     3781 caccaacaac aaaaggagaa aaagacaatt tgcatgtttt ttcctttacc gtaggtaatt
     3841 ctccggcccc cccctgtgaa cattgggtca tattacaaaa aaatttgtga atttgattca
     3901 tgcactatat tatcagtaag tggttgataa acccctgaaa agattccgtt ttatccggaa
     3961 taaagggacg atatatgcaa aaacatatta aacaaagcct caaccaaaac atgagagctg
     4021 tgttatatat caatgcaacg ggagaaattt acctgtgtct tgctttgaaa aaaagagggg
     4081 gaaagctata tgttcgataa taaattaata attttactat gatagtctgc ttcagaccgc
     4141 aacttttcag ttctttctgt acgcttgccc aaagtttaac cactttgttt ttctatacac
     4201 ccaatggttc tatctgcccc ttgatgttaa tccccaaagc caataacatg aatgtattca
     4261 tatcacaact ttcctaccag actctccaat gcaataactg cataattgtt tttagtggaa
     4321 atgtatcgtt ttcttaaaga gaagaataac attccatttt attatgttcc caatttaatt
     4381 atattaatat atattggcat taataggaaa atacatcagg agaaatcaaa tgcagacatc
     4441 aaacataact aaccatgaaa gaaatgactc atcatggatg tcaactgtca aatcaactac
     4501 agaagtaagt tggaataaat tgagtttttg tgacatttta ctgaaaataa taactttcgg
     4561 catatattct ccacacgaaa ctctagccga aaaacatagc gaaaaaaaac taatggatag
     4621 tttctcacca tcactatcac aagataaaat ggacggagag tttgcccacg caaacataga
     4681 tggcatttca atacggctat gccttaacaa aggcatatgt tcggtttttt atcttgatgg
     4741 agataaaata caatccactc aactttcatc aaaagaatat aataacttgt taagttcact
     4801 cccccccaaa caattcaact taggaaaagt acatactatt acagctccgg tatcagggaa
     4861 ttttaaaact cacaagcctg caccagaagt tattgaaaca gccattaatt gctgtacatc
     4921 gataatacca aacgatgatt acttccacgt taaagacact gattttaata gtgtttggca
     4981 tgatatttac agagatattc gagcatctga cagcaatagt acaaaaatat atttcaacaa
     5041 tattgaaata ccactaaaat taattgcaga tctaattaat gagttaggaa ttaatgaatt
     5101 cattgatagc aaaaaagagc ttcaaatgct aagttacaac caagtaaata aaataatcaa
     5161 ttcaaacttc ccccaacaag acctatgttt tcaaacagaa aaacttttgt ttacttcact
     5221 ctttcaagac cctgcattta tatctgcact aacatctgca ttctggcaat ctcttcacat
     5281 cacgtcttcc tctgtggaac acatatatgc ccaaattatg tccgaaaaca tagaaaacag
     5341 gcttaatttc atgcctgaac aacgagttat taacaattgt ggacatatta taaaaatcaa
     5401 cgcagttgtc cctaaaaacg acactgcaat ctctgcctct ggggggaggg cttacgaagt
     5461 tagctcatca atattaccat cgcacataac atgtaatggt gttggtatta ataaaataga
     5521 aacaagctat ctcgttcatg ctggaacact tccaagcagt gaagggcttc gcaatgctat
     5581 ccctccagaa agtcgtcaag tatcatttgc catcatatct cctgatgttt aagccaatat
     5641 tatattggct gccagactgt aaaaaaacgt tcttcagaac agctctataa actattaaac
     5701 caaagctaaa aggatgggga ccaatagaat ctcagcccat cctttttata tcattatgaa
     5761 ccgccccgga aatcctggag actaaactcc ctgagaaaga ggtaaacagg atgactaaaa
     5821 atacacgttt ttcccctgaa gtccgtcaac gggcagttcg tatggttctg gaaagtcagg
     5881 gcgaatatga ctcacaatgg gcagcgattt gttccattgc cccaaagatt ggctgtatac
     5941 tggaaactct gcgtgtctgg gtacgccagc atgagcggga taccgggggc ggtgagg
//
LOCUS       DAJTPG010000168         5785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTPG010000168 DAJTPG010000000
VERSION     DAJTPG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(1..308)
                     /locus_tag="OO230_003802"
                     /pseudo
     CDS             complement(1..308)
                     /locus_tag="OO230_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(647..1201)
                     /locus_tag="OO230_003803"
                     /pseudo
     CDS             complement(647..1201)
                     /locus_tag="OO230_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1344..1466
                     /locus_tag="OO230_003804"
                     /pseudo
     CDS             1344..1466
                     /locus_tag="OO230_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1728..2294)
                     /gene="ipgB2"
                     /locus_tag="OO230_003805"
     CDS             complement(1728..2294)
                     /gene="ipgB2"
                     /locus_tag="OO230_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5693381.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2576..2890)
                     /locus_tag="OO230_003806"
     CDS             complement(2576..2890)
                     /locus_tag="OO230_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693382.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2968..3078)
                     /locus_tag="OO230_003807"
                     /pseudo
     CDS             complement(2968..3078)
                     /locus_tag="OO230_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3402..4079)
                     /gene="ospD1"
                     /locus_tag="OO230_003808"
     CDS             complement(3402..4079)
                     /gene="ospD1"
                     /locus_tag="OO230_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5693383.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4451..4588)
                     /locus_tag="OO230_003809"
                     /pseudo
     CDS             complement(4451..4588)
                     /locus_tag="OO230_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4670..4968)
                     /locus_tag="OO230_003810"
                     /pseudo
     CDS             complement(4670..4968)
                     /locus_tag="OO230_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5557..5716)
                     /locus_tag="OO230_003811"
                     /pseudo
     CDS             complement(5557..5716)
                     /locus_tag="OO230_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1589 a         1247 c         1148 g         1801 t
ORIGIN      
        1 aagggcaaca tcagcgtgag cgactggcgc agatgttatc cgccggggcg ggagcttttt
       61 aatgtccgta tgacggtatt gatgccgatt ttcagcgtac ggccagtatc gcgaactccg
      121 gcaccgttga aagccatttc agtgatttgg tttttaactc ccggtttacg ggcctcgtaa
      181 cagtaagtga gctgaaacac ccggtgacag tcacggcaac ggaatcgatc atgtcctttt
      241 gggttctgcc cgtgacggta aacctgaacg gactggcaac gggggcaatg aaagttaact
      301 gtgaccatga aagaacctaa aagcgggtat tataacccct aattcaacta attggaggca
      361 ttgtcatttt taatctatgg atacacttaa aaatcctctc accttacgtt aaaaaccttc
      421 tcatgtttag agttttttat tatgtttatc ataatgatat gacgatctga gtttttaaaa
      481 tcaagaagaa aagataagtt ttttaaatgc acagtgcctg tggataactt tgcgtaaatc
      541 cggaggacag atcactctga agcaagacaa cagcatctga aaacaatatg ctcacgtttc
      601 aaccacaggg cgtaagtgat cctacccacg taatatggac acaggcctaa gcgaggttct
      661 ggttttcaaa ttgttccgga ctgaggccgc cacaccaact gtgccgtcgc cagcgattgt
      721 aatcacattc gatataatta aacactgttg ccctcattat ttcccggctg ataaagtgtt
      781 ctccgtggag acattccact ttcagcgaat gaaagaagct ttccacgcag gcattatcgt
      841 agcagcagcc ctttgcgctc atactgccac gcagattatg ccacttcagc agcgcctgat
      901 aatctgctga acagtactga cttcctcggt ccgagtgaac gataacgttc cggggtctct
      961 tacgccgcca cagcgccatc tgcagggcat cgcaggccag ttgcgccgtc aggcgtggtg
     1021 acatggacca gccaataacg gcacgtgacc acaggtcaat gaccacagcc agatacagcc
     1081 agccttcatc tgtacgtaag tacgtgatgt ctcccgccca cttctggttc ggaccactgg
     1141 cgtaaaaatc ctgctccagc agattttctg acacaggcag gccatgtgcg cggtaactga
     1201 ccgtattgct gggtaagatc aatttgctca ctggtattta ctacgtgcat cgaatgagtg
     1261 atcaagagcc gtatgacgcg agagtgtcac gtacggttcc gtaagcagcc ggagagtgaa
     1321 actctctccg gctgactcga ctcgcaggtt ccgacagcgg tgacgaacat gcagcggtgt
     1381 tgttctcgct gatcggcaca tgccgtctga acaatgtgga gccagaaaaa tggctgcgtt
     1441 acgttattga acatatccag gactgaccgg caaaccgggt acgcgatctg ttgccttgga
     1501 aagttgatct gacttctcag taaatatcaa tatgattctg acgagccgct tacctttgag
     1561 cagagaaagg ggagagggat cccacatcca tgagctagga aaatacaaaa actggctata
     1621 caacaattca atatattagg acttacttgc gaggagaaat atagcaccgc tcaggcaatt
     1681 cctgggtatt tacttttctt agtttcttta ctcgggatgt tctgtcatca gaaaggcgat
     1741 tctaaatttg taatatagtc actcatattc ccacgtaatt tctttagatc aatatattgc
     1801 acatttttct caaaaaactg atcggaaata agatttgcaa cattgcttgt tgtatcattt
     1861 ttaacgcttc tattcatccc ttcacaaagt tcgtccattt tctttttgaa ataactgctt
     1921 cctgatacaa gatgatttat ggaactttgt gcagcattga tgtctagaga aatgcgataa
     1981 tctttctcaa cctgtttaaa tatctgttta cggagattat cgccgatttt tcggctgtgt
     2041 tctgcacaaa acttatcaat acattggttt accacccggg atacgtagac ttttttttca
     2101 tcatttttcc aatctgtaat attttcaccg atttgttctg aaacagagct caaaatacta
     2161 gtatttattt tggttgatga tatttttact atccttttcc ccattgtaca gcggataatc
     2221 tcatccacct ttcctgaaaa atatcttttg tgagataaac tgattccaaa attattaaaa
     2281 gatgttccaa gcattataat caccttgtca tgtaaaatag taacattgtc attattttag
     2341 ctgattcaaa caattctgta aatgctttga gttctttact cccttggctc aatgatggac
     2401 cgtctggaat cccagagact aaactctctg agaaagagag tatgaaaaaa cttggggcag
     2461 tccctaaatg aagggaccaa cagcggtaaa ataaccggca tcattcataa tgatgccctg
     2521 ttagttgtag ttatgaagat ttacgtcatt aaaattgctg ttcgtagtgt cagtcctatg
     2581 gtctggcatc agctgaggat cgtagccgat acatcactgg cttcacgaca ttcgtgttga
     2641 ggctattcat gaaaactcca cgctgaaaac gccgttttgt ataaacggtc acggtatgcc
     2701 cggagccact gttgctgata aattcgataa aaccttggcg tttcttgaag ctatcgttaa
     2761 tgcggataat gaaacaacga ttggtgagat tcgctctttt gccgacgccc tggacgctgt
     2821 ccggttcaat cgcaacaaaa tcaaccggca gctgagcaaa cccaaccttg cctctctggc
     2881 gctggagcat gaagtgattt ggctgggacg tagccgctga cagcttctat aaaagtatga
     2941 tcctacccac gtaatatgaa cacaggctta agtgaggttc tggttttcaa attgttcagg
     3001 actgagacct ccacaccaac tgtgccgtcg tcagcgattg taatcgcact tgatataatt
     3061 aaacactgta tatttcattt gtggagtctc cagtctgaga gggctttatg cattccgtat
     3121 tgggcactat gatgccagaa atctgcaagg ctccacacct tcctgtattt cccttcctct
     3181 atcacagtgc actttactta atagggtggg atgtgttcat tacatttatt gtgtttttta
     3241 tgattgtttt tgcgtccagt gtcgttcgtc atgggaaact ggttctatgg gtagtatcgc
     3301 tcagtctagt taactgtttg tttcttttta gaaattaagc agatcacaca attcggggag
     3361 agatccttct aagtgcaatt ctttgcagct atttagcaca cttaaaacaa ctcttgtatt
     3421 ttgtcagaaa taatattaaa catgtcttgt tcattattgt taagtctgtc tgctatttct
     3481 gtaagtttta tcccactatg atggcatata tttatatatg ccatcatagc ctcgatattc
     3541 cctttccctg cggccagaaa taatcctggt aacccctcat tatctcttac atccaatagt
     3601 ttaatcgttt cttttggtga taaatcaaaa gttttaataa aatctccata agcttgaatc
     3661 gcagcggaat gaccttcttg aagagctatg aacaaagcag attccccagc attatttcgt
     3721 gctgcaagaa cttctagttt ttcctctttg ggtatttcgg ctgccaacag cgattcaccg
     3781 tatttacgcg ctgcatcggc atttccatgc caaatcaatg aaaacaaatt atggcggtta
     3841 tactccttat tattctttgt cggaatggat atgttctgta ttttggccaa ttttctgaaa
     3901 gaaacaccac tatatccccc ccctgatttt tcttcattga tggcgtggga tatagcggta
     3961 tttgtcacgt taatgatatt atttttcatt cctttatttt cattagcagt attgctgcct
     4021 attattaagt gcatattttt gttgtttgct ggatgtaatc catagttatt tattgacatt
     4081 tttgttcatc ctttttgcta aaaaaaaaca ataacctatt tttgtaatgt aatagatatt
     4141 agtctattgc taatctaaaa aaaccattgc cacattgaat ggctatattc accaataaat
     4201 tctttcgatt tcgtatattg acatatcgta gatatatctt atccctaaga ttcaataatc
     4261 gagtccgttt accattctgt gcaagcgcct tcactcagaa gtgaccgtca aggcgatcac
     4321 caaacttgat aataaagcag ctcattgcca tacgccagtc cttaaggggc gttgtccatt
     4381 tctgtgatgc tgcctagatt taactgccag ctccggctta tctacatcta aatatgtgag
     4441 cctaataccg ctacactttg tccagactat gtttttctcc gggaagtggg ctaaccgtct
     4501 ccataaagtg acgaactttt ttcaacagtg gccatattga acggcactga tgattacggg
     4561 ttattgtctc atgaagagcc ggccacatca ctgacagcag tgacttcagg ccttcagcac
     4621 cagacagcct gaaccagtta atccatcgcc cgacaaattt agtaatctag ggctggtttt
     4681 tcttcagctg ttgcggttcg aaagtgccat tacgatcacg cagagtacgg atttccagag
     4741 gttcatctcc agtagtgata atcttcgtta agcaaccatt gcgggaattg ctgccagatc
     4801 taagctgatt tttctcgcac ccaagatgat gagtcatatt agcattgagt acggtttcat
     4861 tttcagcatc caatcaaact gactgagatc ttcaggattt ttgagacttt tggctagttc
     4921 attagctaaa gcccgcaact tattagatat caactgtttt tcattcataa attcaccttc
     4981 atttgatatc aaattaaata gttcaacatc agagaattat acaaacttat gtacaggctc
     5041 ctatgatctt cgaatcctga agattatagt gaaaataagt tttcacgttg aaatcccaca
     5101 tagttagtat atgataaata atcatggatg acctgattaa cctcttgggg tagtgctttt
     5161 atagaagaat aaatattcta ctaatttaat acatcaccta aaatagtatt gttttttatg
     5221 gttggttgaa ggtcgtggtg atgatgttag tccttttgag aatattccag tgccagatct
     5281 tacttataat aaaggggctt ggcagtttgg ataaactatt gttcttattc cactaataca
     5341 aactttttgt aaagtatatt aaatatactt catttcatac tgaaatcccc ggaagtattc
     5401 aatcaaacaa ttacaccata aaaggaagag ggggccctaa ttaagttgtc aagcatgttt
     5461 atgatacctg cagggtataa attttctggc tgcaagcaga cacatataaa acgtctttat
     5521 tgatcctacc cacataatat ggacacagtc gtaagtgagg ctctggtttt caaattgttc
     5581 cggactgaga ccaccacacc aacggtgccg tcgccagcga ttgtaatcgc actcgatata
     5641 actaaacact gttgcccgca ctatttcccg atggataata gtgttctccg tggagacatt
     5701 ccattttcag cgaatgaatt ttcatctgtt gctgtatcca gtcatacagt gactgcatca
     5761 gtggcgcggc tctggctttt cttgc
//
LOCUS       DAJTPG010000169         5783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTPG010000169 DAJTPG010000000
VERSION     DAJTPG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            441..1829
                     /gene="narU"
                     /locus_tag="OO230_003812"
     CDS             441..1829
                     /gene="narU"
                     /locus_tag="OO230_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5693384.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIAMIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1911..5651
                     /gene="narZ"
                     /locus_tag="OO230_003813"
                     /pseudo
     CDS             1911..5651
                     /gene="narZ"
                     /locus_tag="OO230_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5648..>5783
                     /locus_tag="OO230_003814"
     CDS             5648..>5783
                     /locus_tag="OO230_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693385.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     E"
BASE COUNT         1366 a         1519 c         1484 g         1414 t
ORIGIN      
        1 accttagatg aaataaacaa tacttactat aataacgatt attacaatca ggcaataaga
       61 gaggaaccga tagactttct ggacagatcg ttttcctcca attcatggcc tttttagtga
      121 ataaatcatc actaattcta taagtgtctg gtcttgttga gaatatagtt aatatgtaac
      181 cctttagatt cacgatctga agggttgaaa catgacatac gttcaaagat taacccccgt
      241 ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta atccccattc
      301 tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc
      361 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat
      421 gtgtgcatat gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
      481 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa
      541 gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta
      601 gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt
      661 tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg
      721 tgcctatatt cggtggacgc cgatggccgg tttttagtac tgcaatcctg attattcctt
      781 gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta
      841 tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt
      901 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
      961 taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct
     1021 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     1081 catggatttg ggtaccgcta ctggcgattg ccatgatcgc cgcctggtca gggatgaatg
     1141 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     1201 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     1261 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     1321 ttggcccatt tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg
     1381 gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt
     1441 tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg
     1501 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     1561 ttcgccagat aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga
     1621 gagaagccgt caccgaaacg gcggcaacgc tgggctttat ctcagccatt ggcgcagtgg
     1681 gcggcttttt tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg
     1741 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt
     1801 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca accatgtcgc
     1861 atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac
     1921 ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac
     1981 aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca
     2041 aaatcgtgcg ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg
     2101 ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg
     2161 acctgcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt
     2221 acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg
     2281 aagccctcaa acaacacagc gatccggtac tggcgtgggc atcgattatg aatgatccgc
     2341 aaaagtgcct gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc
     2401 aggaactaaa ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg
     2461 atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa
     2521 cgcgttatct gtcgctgctt ggcggcacct gttaaagttt ctacgactgg tattgcgacc
     2581 tgccgcccgc ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact
     2641 ggtataactc cagctacatc atcgcttggg ggtctaacgt accgcagaca cgtacgccgg
     2701 acgcccactt ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg
     2761 actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata
     2821 gcgccctggc gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca
     2881 gcgactactt tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg
     2941 agcctcgcga cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg
     3001 atggactggg cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg
     3061 aattggtagt gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc
     3121 tggaatccat tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg
     3181 acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca
     3241 gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg
     3301 atggcaacac ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg
     3361 atcgcgggct ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc
     3421 cagcctgggg tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg
     3481 aatttgccga taccgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg
     3541 ttaaccactg gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct
     3601 gcggttgtgt cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc
     3661 gcccacaaac cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc
     3721 agatgaacag cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct
     3781 ctgctcagga gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg
     3841 atttcaacgt tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta
     3901 acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg
     3961 cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca
     4021 atcatccgcg taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc
     4081 acgagtatat acagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg
     4141 gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca
     4201 agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata
     4261 tcgttctgcc caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc
     4321 catttattca tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg
     4381 aaatctacaa aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag
     4441 aaaccgacgt ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt
     4501 gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga
     4561 atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc
     4621 cattgatgga caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa
     4681 tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc
     4741 tgattgacac cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg
     4801 gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc
     4861 atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc
     4921 gtaaaattat ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg
     4981 cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc
     5041 tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc
     5101 ctatcgacac ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa
     5161 aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa
     5221 acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg
     5281 cccgagaact gaccattgtc gataacgact gggtggaagt atttaacgcc aacggcgcgc
     5341 tgactgcccg cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg
     5401 cccaggaacg cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc
     5461 ataactcggt cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc
     5521 tggcctgggg ctttaactac tacggcaccg tcggttcgaa ccgcgacgaa ttcatcatga
     5581 tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg
     5641 cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt aacctcgata aatgtatcgg
     5701 ctgccatacc tgttcggtga cctgtaacaa cgtctggacc ggacgcgaag gcatggagta
     5761 cgcatggttt aacaacgtcg aaa
//
LOCUS       DAJTPG010000170         5590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTPG010000170 DAJTPG010000000
VERSION     DAJTPG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..400
                     /locus_tag="OO230_003815"
     CDS             <1..400
                     /locus_tag="OO230_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5693386.1"
                     /translation="QRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYY
                     SDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGIDPEA
                     YLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            432..1046
                     /gene="istB"
                     /locus_tag="OO230_003816"
                     /pseudo
     CDS             432..1046
                     /gene="istB"
                     /locus_tag="OO230_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1215..5309)
                     /gene="sepA"
                     /locus_tag="OO230_003817"
     CDS             complement(1215..5309)
                     /gene="sepA"
                     /locus_tag="OO230_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5693387.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1376 a         1398 c         1329 g         1487 t
ORIGIN      
        1 gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga agaatggtac
       61 gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc agtgggatgc
      121 cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag cagaaagagc
      181 gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg atcatggagg
      241 cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg gtatcgatcc
      301 agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca accgagttga
      361 cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac ggccttccgt
      421 tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat aaaaaggagg
      481 cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg ccggaactga
      541 atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct ggagaaaacg
      601 ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg atcgtggatg
      661 gcgtagtagg ccagggctac cgggcccggt tctacagcgc aggagagctg ttgcaggaac
      721 tacgtaaagc cagagctcag ttgaaactga atgagctgct actgaaactg gatcgctacc
      781 gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa acgggtgtgc
      841 tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc agtaaccatc
      901 cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg gcagcagacc
      961 gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg aaaaagacag
     1021 cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt atagttggcg
     1081 ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag aaaaggcatt
     1141 tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc cgaatggcag
     1201 acctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt atccacatta
     1261 tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc cttaatttcc
     1321 gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa acgtttctct
     1381 ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg ataacccagc
     1441 ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac gtcaacacca
     1501 gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat tccccggtct
     1561 tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg tggctccacc
     1621 caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggcata ccaggaatga
     1681 gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt atcatgatgg
     1741 agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga ggcatacaga
     1801 ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt gctgtccgta
     1861 taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt tctgtcgaca
     1921 ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc ggtaccattc
     1981 attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc catacgtttg
     2041 ttcaggttat tgacctctgt aaggaaggac ttataacctg atgccataaa gtcttttgcg
     2101 gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca ttttgtggca
     2161 tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat ctgctgcgta
     2221 ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt cacgctcatt
     2281 tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc tgacagtttg
     2341 tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa cgtggtatta
     2401 tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc cagttcaatt
     2461 cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta ccacagagca
     2521 ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac agcgttatat
     2581 cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc atcactgtct
     2641 cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag atagcccata
     2701 tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt agaaattacg
     2761 ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag agaagcattt
     2821 tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag ttttgctcct
     2881 tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac agcattctga
     2941 gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct ctccatgatt
     3001 tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg gtcaccaact
     3061 gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc gtcactcctg
     3121 tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc gttaatatct
     3181 ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt cagtttttcc
     3241 agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt tccagcatga
     3301 ataactggat gaccctggaa tatcagcgtg ccggacttct tggttatcga accttctggc
     3361 aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg aatattcaca
     3421 tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc ctcaattttt
     3481 tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg ccagacactg
     3541 ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat gaaatattgc
     3601 ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa aatactgaca
     3661 gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag tgttaccgtt
     3721 gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg aagacgggta
     3781 aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca cgaaatatta
     3841 tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct ggcaatacca
     3901 acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag aataacggta
     3961 ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt cagggtacct
     4021 tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag aacgtgggtt
     4081 cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc ggacaccctg
     4141 taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg cagaacgacc
     4201 tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt tcctttcttt
     4261 ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt agccgcatca
     4321 tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc aaaatcattt
     4381 tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc atagttagtt
     4441 cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata agcaaacagg
     4501 ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc gggattaaaa
     4561 atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag cgcgctgacg
     4621 gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc atttttgtct
     4681 ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc gccggcctga
     4741 taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc tgtcaccagt
     4801 ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc aaccgcagta
     4861 taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg tttggcggta
     4921 accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc ggtagtaata
     4981 gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag attgccctgc
     5041 ttgtcgtaaa tggaaatatt cgtggtcccc ggcgtaaact gacctttatt ctcggcaaag
     5101 tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc tggccatgcc
     5161 gaggaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag tctgcgatgg
     5221 cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt ggttatatgg
     5281 caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt tgacaaagat
     5341 taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat tattaagcat
     5401 ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac aaccaaaaaa
     5461 aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga tactcttctc
     5521 ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat aaggggcagt
     5581 ttggatatcg
//
LOCUS       DAJTPG010000171         5584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTPG010000171 DAJTPG010000000
VERSION     DAJTPG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            431..1339
                     /locus_tag="OO230_003818"
     CDS             431..1339
                     /locus_tag="OO230_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5693388.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1385..3376)
                     /gene="tkt"
                     /locus_tag="OO230_003819"
     CDS             complement(1385..3376)
                     /gene="tkt"
                     /locus_tag="OO230_003819"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5693389.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3654..4412
                     /gene="loiP"
                     /locus_tag="OO230_003820"
                     /gene_synonym="yggG"
     CDS             3654..4412
                     /gene="loiP"
                     /locus_tag="OO230_003820"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5693390.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPADLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4618..5538)
                     /gene="speB"
                     /locus_tag="OO230_003821"
     CDS             complement(4618..5538)
                     /gene="speB"
                     /locus_tag="OO230_003821"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5693391.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1379 a         1461 c         1440 g         1304 t
ORIGIN      
        1 gctgaatgta taactgagat tgatgattct ggaaaataat cactaagccg cataaattat
       61 ccttatttta tttttagggt accgtccggc ggtaaaaata aaatactcgt gcggtttaat
      121 gaaagcaatt gggtacgcga gaagaaaaac cccacaaacc aaaataataa aaccttaaat
      181 aacaacaata tgaaattgat atttttaaag tcaactttgt atttgttatg ttcatcacaa
      241 aatcaacatt ttgtttgtta ttgtatttgt gatgcatatc tctatttatt tattgaatgt
      301 tcgaaaacat acttttctaa tgcaatgttt tttcgctata cccctttata gattctgaat
      361 tctcacctgt cggctgtttt ctcctcatct catgtgacat attcccatac tttactcaag
      421 gaagattgtc atgaaagcac ctgtttcatt aacctcattt cgcccacaaa aatctcttgc
      481 tatcgccatt ggcgtgcttg ccgtggttgt gctgccattt ctctcgtatt acaccgttaa
      541 tgaaggtgaa cgcggcattt tgctgcgcta cggaaaaatc gtaaaagttg ccgaaccggg
      601 gctgggtttt aagatccctt ttatggaaag cgtggagaaa atttccacgc gtaatcaggc
      661 tgtggtctat cagggattac aggcctacag ccgcgaccag caaccggcac aaatgacagt
      721 atcagtcagc ttccacatta aaccttcaga agcaggcgcg gtttatacca cctacaacac
      781 tattgaagca ttaaaagatc gtctaattgt ccgtcagttg ccaactcagc tggagaacat
      841 tttcggccaa tataccgcca tcagcgccgt acaggatcgt acaaaattgg ttcaggatct
      901 gcaaaatgcc atgcgcaaag ccgttgttgg gccagtggta attgacgggg tccagattga
      961 aaatattgat ttttctgacg cttatgaaaa gtcgattgaa aaccgaatga aagcagaagt
     1021 cgccattgcc acgcgtaagc aaaatctgga aacagagaaa atccaggcgc aaatagcggt
     1081 aactcaggcc caggcagaag cagacagtaa actggcggca gccaaagcag aagcggaaac
     1141 tattcgcgta agaggcgcgg cagaggctga aactattcgt ttaaaaagtg cggctgaagc
     1201 agaagcaatc cgcctgcgcg gtgaagctct gcgggataat cccggacttg tagccctcac
     1261 cactgccgaa cgttgggacg gtaaactgcc cgataccatg atccccggca gcacagttcc
     1321 gtttatctct actaagtaaa aaaagggccg ctttcgcggt ccttctcaca aaacgtctta
     1381 ctgattacag cagcgctttt gctttcgcaa caacgttatc aacagtgaag ccgaactctt
     1441 caaacagcag ctctgccgga gcagattcac cgaaggtggt catgccgacg atagcgccgt
     1501 tcaggccaac atacttgtac cagtagtcag caatacccgc ttctaccgca acgcgtgcag
     1561 taaccgcttt cggcagtacg gattcacggt aagccgcatc ctgcttgtca aatgcgtcgg
     1621 tagacggcat ggacaccacg cgcgctttca cgccttcggc agtcagtttt tcgtaggcag
     1681 caaccgccag ttcaacttca gaaccggtgg cgatgaagat cagttccggc tgaccagcac
     1741 agtctttcag cacataacca ccgcgcgcga tgtttgccag ctgctcttca gtacgttcct
     1801 gctgcgccag gttctgacgg gagaggataa gcgcagtcgg gccgtcctga cgctcaacgc
     1861 cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg acgccatgta gacatgttcg
     1921 gggtcacgcg cagagaagcg acctgctcta ccggctggtg agtcgggcca tcttcgccca
     1981 gaccgatgga gtcgtgggtg taaaccatca cctggcgctg tttcatcagc gcagccatac
     2041 gtacggcgtt acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaagc
     2101 caccgtgcag ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt
     2161 ggatgtagtt acctgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt
     2221 tagacggtgc caggtcagca gagccgccga ggaattcagg caacagcggg ccgaacgctt
     2281 cgatagcatt ctgcgacgct ttacggctgg cgattttcgc cggattagcc tgcagtttag
     2341 cgataaactc tttcgctttg gcgtcgaagt cagacggcat ttcgcctttc atacggcggg
     2401 taaattcagc cgcttcctgc ggataagctt tcgcgtaagc cgcaaacttc tcattccatg
     2461 cagattcttt cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga
     2521 tttcgaacgg tgcatatttc cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc
     2581 ccagcggcgc accgtgggag tcgtgagtac cggctttgtt cggggaaccg aaaccgatga
     2641 tggttttgca catcagcagg gacggtttgt cagtcactgc gcgcgcttct tcaactgcgc
     2701 gtttgatgga tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt
     2761 cgaaacgcat tgctgtgtcg tcggtgaacc agccttcaac gtgaccatcg atagaaatac
     2821 cgttgtcatc gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa
     2881 cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt
     2941 cgacaatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca
     3001 taccgactgc gttggcaata ccctgaccca gcggaccggt ggtggtttcc acaccagcgg
     3061 tataacctac ttccggatgg cccggagttt tagagtgcag ctgacggaag tttttcagtt
     3121 cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt
     3181 ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt
     3241 tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt
     3301 gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct
     3361 ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt
     3421 gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc
     3481 gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt
     3541 gcgcaatctg ctcgcccgtc cgctgcgctt ttccttatac tgagactgag cgtcgattca
     3601 cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa
     3661 ttcgcgcctt attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg
     3721 actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg
     3781 cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg
     3841 cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca
     3901 atatcaacgg tcagccggtg aattacaaag tgtatatggc gaaggatgtg aacgcctttg
     3961 caatggctaa cggctgtatc cgcgtctata gcgggctgat ggatatgatg acagataacg
     4021 aagtcgaagc ggtgatcggt cacgagatgg ggcacgtggc gttaggccat gtgaagaaag
     4081 ggatgcaggt ggcgcttggt acaaatgccg tgcgagtagc tgcggcctct gcgggcggga
     4141 ttgtcggcag tttatctcaa tcacaacttg gtgatctggg cgagaaatta gtcaattcgc
     4201 aattctccca gcgccaggaa gcagaagccg atgattattc ttacgatctt ctgcaccaac
     4261 gcggcatcag cccggcagat cttgccacca gctttgaaaa actggcgaag ctggaagaag
     4321 gtcgtcaaag ctcaatgttt gacgaccatc ctgcatccgc cgaacgtgcc cagcatattc
     4381 gcgatcgcat cagcgcggat gggattaagt aaagcctagt ggtgtcgaac gtaggtcaga
     4441 taaggcgttc acgccgcatc tgacattaat ggcacatttg ttaccttgtg cgcattgccg
     4501 gatgcgatgc tggcgcatct tatccggcct acgggtgccg aacgtaggtc ggataaggcg
     4561 ttcacgccgc atccgacatt aatggcacgt tttacccgtg cgcatcgcat ctggtgctta
     4621 ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg ccgcagccag
     4681 agcggtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacgat
     4741 gttgagatct ttcaggccgc gtaccagttt aatggcgcga tcggaggtca ggccgccaat
     4801 cactggcgta ccggttcccg gcgcgaaagc aggatccagg cagtcgatat caaaagtcag
     4861 gtaaaccggc atatcaccca caatctgttt cacctgggcg ataacgtcat ccacgctgcg
     4921 atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctttatcaa actcggtacg
     4981 aataccaatc tgcacggaat gattcgggtc gatcagacct tctttcggcg cggtatagaa
     5041 catagtgccg tggtcaaatt cacaaccgtt cgcataggta tcggtgtggg cgtcaaagtg
     5101 taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
     5161 gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
     5221 ctgcagcttt tcgctcatct cacgggcatc gccaaaggca tataccagat cgccgcagtc
     5281 cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc
     5341 cagattcgtc gaaacctgac ggatcgctgc cggaccgtgg cgaccacccg cacgaccaga
     5401 agtggccata tcgaacggca cgccagtaat cacccagtct gcatcgctgt catacggctg
     5461 gaagttcatc ggcaggcgta aaaaaccaaa ggcattggaa accagtgagt tatcgtattg
     5521 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
     5581 gcgt
//
LOCUS       DAJTPG010000172         5582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTPG010000172 DAJTPG010000000
VERSION     DAJTPG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            27..242
                     /locus_tag="OO230_003822"
     CDS             27..242
                     /locus_tag="OO230_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5693392.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            235..621
                     /locus_tag="OO230_003823"
     CDS             235..621
                     /locus_tag="OO230_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5693393.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            629..1315
                     /locus_tag="OO230_003824"
     CDS             629..1315
                     /locus_tag="OO230_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5693394.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="OO230_003825"
     CDS             complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="OO230_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5693395.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1998..3203
                     /gene="tet(B)"
                     /locus_tag="OO230_003826"
     CDS             1998..3203
                     /gene="tet(B)"
                     /locus_tag="OO230_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5693396.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="OO230_003827"
     CDS             complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="OO230_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5693397.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4042..4413
                     /locus_tag="OO230_003828"
     CDS             4042..4413
                     /locus_tag="OO230_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5693398.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4423..>5582)
                     /locus_tag="OO230_003829"
     CDS             complement(4423..>5582)
                     /locus_tag="OO230_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5693399.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1029 c         1215 g         1796 t
ORIGIN      
        1 cactattaag tcatgtagct ggttttattt caattgaacg ttttcaaagt ctagctacag
       61 aaggaaaaat gttatcgcta tcttggtggg aaaacgaata cgcagttctg caatggaaaa
      121 atcatgtttt acatgcgaaa gctcaacaag aagggcgaga gtcaatattt gatttttaca
      181 aaattagtat tgctcatatt actcgcgaat attcatttaa aaaggacaag gataatgttt
      241 gatgttcacg ttgttttaga taatcaaata ggacaattag cattactagg aaaaacatta
      301 ggtaataaag gtattggatt ggaaggggga gggatattta cggttggtga tgaatgccat
      361 gctcattttc ttgttgaaca aggaaaggaa gctaaaatag cgctagagca agctggactg
      421 ttagtacttg cgatccggac accattaatt cgtaagttaa aacaggaaaa accgggggaa
      481 cttggcgaaa tagcacgagt attggcggag aataacatta atattttagt gcaatacagt
      541 gaccatgcta accaactgat attaataacg gacaatgata gtatggctgc atctgttacg
      601 ctcccttggg caataaagtg aacttgcgat ggctaattta atacgaaaag aggttacctt
      661 tgagtcctca atagccgcga taggggcggc tatgtctgac atttcacgag ttaaaatact
      721 cagtgctttg atggatgggc gagcttggac ggccactgag ctaagttctg tggcgaatat
      781 atcagcttca acggcgagca gtcatttatc taaattatta gattgccagc taatcacagt
      841 agtagctcaa ggcaagcatc gttattttcg gctagcagga aaagatattg ctgaattgat
      901 ggaaagtatg atggggatct ccttaaacca tggcgtacat gccaaagttt ccacgccagt
      961 gcatttacga aaagcacgta cttgctatga tcatttagct ggcgaagttg ccgttaagat
     1021 ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga tcacattaag
     1081 tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt cacgtaaaat
     1141 ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt acgttggagc
     1201 cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg gttaccgtga
     1261 agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca tttaagttgt
     1321 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
     1381 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
     1441 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
     1501 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
     1561 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
     1621 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
     1681 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
     1741 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
     1801 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
     1861 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt
     1921 aattcctaat ttttgttgac actctatcat tgatagagtt attttaccac tccctatcag
     1981 tgatagagaa aagtgaaatg aatagttcga caaagatcgc attggtaatt acgttactcg
     2041 atgccatggg gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg
     2101 cttcggaaga tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg
     2161 ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt
     2221 tgttgtcatt aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt
     2281 ggatgctgta tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg
     2341 catcggtcat tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag
     2401 gggcaagttt tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga
     2461 tttcaccgca tagtcccttt tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg
     2521 ttatgttttg gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg
     2581 ttgagacgca atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga
     2641 ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta
     2701 ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc
     2761 ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa
     2821 aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta
     2881 tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt
     2941 tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac
     3001 agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg
     3061 ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt
     3121 tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga
     3181 gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc cgcgaaatat
     3241 aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt tagcttcaaa
     3301 taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt aaatagcaat
     3361 aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg cgatatgcat
     3421 aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttaa gttggttctc
     3481 ttggatcaat ttgctgacaa tggcgtttac cttaccagta atgtattcaa ggctaatttt
     3541 ttcaagttca ttccaaccaa tgataggcat cacttcttgg atagggataa ggtttttatt
     3601 attatcaata atataatcaa gataatgttc aaatatactt tctaaggcag accaaccatt
     3661 tgttaaatca gtttttgttg tgatgtaggc atcaatcata attaattgct gcttataaca
     3721 ggcactgagt aattgttttt tatttttaaa gtgatgataa aaggcacctt tggtcaccaa
     3781 cgcttttccc gagatctcat ctattgaaac agcttgatag cctttttcaa caaacaatat
     3841 tcgtgctgag ttaaccagtg attgataggt actcttaaaa ttttcttgtt gatgattttt
     3901 attttccatg atagatttaa aataacatac cgtcagtatg tttatggtat catgatgatg
     3961 tggtcgtgac aatcttaaga acatttaggt tattttatgt atattgaaca gcattctcgc
     4021 tatcaaaata aagctaataa catccaatta agatatgatg ataagcagtt tcatacaacg
     4081 gttatcaaag atgttctatt atggattgaa cataatttag atcagtcttt actgcttgat
     4141 gatgtggcga ataaagcggg ttataccaag tggtattttc agcggctgtt caaaaaagta
     4201 acaggggtca cactggctag ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag
     4261 ttgaggttga cgaaaaaaac tatccttgag atcgcattaa aatatcaatt tgattcccaa
     4321 caatctttta cacgtcgatt taagtacatt tttaaggtta caccaagtta ttatcggcgt
     4381 aataaattat gggaattgga ggcaatgcac tgagagatcc cctcataatt tccccaaagc
     4441 gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct
     4501 tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac
     4561 gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg
     4621 aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa
     4681 acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa
     4741 gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg
     4801 tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg ccgacgctga
     4861 gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc
     4921 tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct
     4981 cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt
     5041 ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta
     5101 ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat
     5161 gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc
     5221 tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc
     5281 gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa tatcagacca
     5341 gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta
     5401 tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat
     5461 gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt
     5521 tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa
     5581 tt
//
LOCUS       DAJTPG010000173         5303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTPG010000173 DAJTPG010000000
VERSION     DAJTPG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..877)
                     /locus_tag="OO230_003830"
     CDS             complement(<1..877)
                     /locus_tag="OO230_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5693400.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTY"
     gene            complement(889..1378)
                     /locus_tag="OO230_003831"
                     /pseudo
     CDS             complement(889..1378)
                     /locus_tag="OO230_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1382..2602)
                     /locus_tag="OO230_003832"
     CDS             complement(1382..2602)
                     /locus_tag="OO230_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5693401.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2606..3349)
                     /locus_tag="OO230_003833"
     CDS             complement(2606..3349)
                     /locus_tag="OO230_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5693402.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3240..4706)
                     /locus_tag="OO230_003834"
     CDS             complement(3240..4706)
                     /locus_tag="OO230_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5693403.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4706..4939)
                     /locus_tag="OO230_003835"
     CDS             complement(4706..4939)
                     /locus_tag="OO230_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5693404.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(4927..>5303)
                     /locus_tag="OO230_003836"
     CDS             complement(4927..>5303)
                     /locus_tag="OO230_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693405.1"
                     /translation="GSVVFWADEWKAKEDELLKSCQRTYQAALEREADIVYDSIGVGA
                     SAGAKFSEINADRKSENAYARRVNYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAW
                     WLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1132 a         1498 c         1332 g         1341 t
ORIGIN      
        1 agtaagtgac gatctgatac agaccgcgat actgcaccgg cacgctctga agcggaacca
       61 gcgggtagcg gacgtatttt ttatcgttgg tgtacgcaac tatgcgatcc ttattcccca
      121 caccacggcc tttcagccat ttaaccgcgc ggatattcag cggaacaccg ttctggtgat
      181 agctgatggt gttggtctga aggttcgtca acagggactg gttacctgca gatgaaacga
      241 tgatgctgga caacagagca aactgctcag gcgggatcag caaatcacgc ggaaccacag
      301 agtaaccaga agcggcccac gcatcagaca gcacctggtt aatgcttgcg cggatttcgt
      361 ccggtgttga ggttgcccac gttttggcag cgttgttgac aggcacgccg tccagggtaa
      421 caaggccttt caggtttaat gcggaatcgc caacatatac ctgttcatcg ttatccatct
      481 gccatttcag ttgcataccg tcatacttct gcgtatcaat cgggcggccg acctgctgag
      541 cagcctgcaa ttctatgacc gtccagccaa gttccatccc ccacaggttc agcgggttac
      601 cggatttgcc gatatccacg ttcacgccag caatagcggt tgagtctttg cctacccagt
      661 tttttccatt cggatttgcg ccagtacccg cagcggcgaa gctggtattc gtccagctgg
      721 aaatgtcatc tgcgatggag gcgtcttcac gcagttgaat atcgcggctc caggtgtacc
      781 ccaccagtgg caggttcagc gtctggtcga gtctctccag ctccccgatg agaaaggcac
      841 cagagctgtc aacggttgcc tgatcaaaag taatcattcg tctgttcctt aaatcttcca
      901 ggaaatttct gcattgccgt cagcatcacc ggcacctgtg aattcagcgt tggtcagcac
      961 cacgtttttg ccactgactg acgtggacat gaatccaccc agaggcactt tgatggattc
     1021 atcagtggag atcgcctttt ttgatagtgc tggcatcaaa atcagaaccg agattaacgg
     1081 tcacgtagcc acgcttcatg gcgtcgcccg ggaagttctt gcctgttccc acctggcgaa
     1141 ccatgtccgg ctgcgacgtg gtcggataag ggcgcacgta gatccccttc accttgtctg
     1201 cggtatcacc atctgccagc ggcacgaaaa aaccgtcatc atcgtattta ccagccaggc
     1261 cataggcagc gaaggcgtta tcggatttaa ggaccaccgg ttcgacggtt aagtcctgcg
     1321 ggcgagagac agccccggca atgccaacag gcatccggta cagaaataca ttattcattt
     1381 tttacccttt acggtttgcc cagaattcag cgttttgttt gttcagggaa gcgatactgg
     1441 tcatgcccat gtttaggcgc tgtgcatcgc cggtggtggc gcgggtgttt cgccctttgg
     1501 caacctcaga cacggcatta aacgccatgt cgaccgattg tttcggtaat ttgcggatat
     1561 ccgcatcacc gactatctgg cgaaccagcg ttttgtcagc agaagccaga acctcgcgtt
     1621 tgaacgcggt cggtttcatc ttacggctca gatcgatacc cgggacgata acttcggcac
     1681 gccaggctga gtcaccagta atcgtggttt cctcttcatc gtcctcgccg tcaccggtcg
     1741 gattatcgtc aggcttatta tcgttatcgc ccgtggcatt tctttccagc ttagccagca
     1801 gggctttcag taatgttttg aggtcatcat cactgtcgcc ggttggatct ccgcccatct
     1861 ctggtgcttt gtccggtagt ggttgctgcg gggacaggtt gatgttgaga ttaacgccct
     1921 gcggcaaatc cccctcatct cctgtaaccg atgcgggagc cgactccacc agttcgttca
     1981 tggtgtcggc atctcctgtc ttgatggccg cacgcatgcg gttccaccag tttttctttt
     2041 gatttgccat tgtgtctctg tctccaattg cacaacgatt tccggctctg cctttaggga
     2101 caagagccac atggtttccg gtaatatcga cctgctcggc tttacctggc tcggtctgct
     2161 cgtactccgc gtcatagccg cacgacactt cgcgcaggcc atcttcgata agctgaatgg
     2221 cgctttcgtc tttgacgata aggtcagcca gcatcaaatc agactgctca cccgtcccgc
     2281 gccggacatt ctggaggtgc ccgacagcaa gcactttcca gttctcggga tttaccagcc
     2341 gcacattccc gttttcatct tcaggatgca ggatcgtgat gctcatccct tcgaatgagg
     2401 caagcgtggc cggatggaat acctgctcag gagaacgcgt gacgactatt tcaacgaact
     2461 tatcgggttt cagttttggc aggtcatcag caccatagag ctgcttacct gttcgtccta
     2521 tcggcacgtc tttgcacagc aacgagccgt cagccaggcg gtatcgggtt tccccgagcc
     2581 tggtctcaaa aaagtatttc atgggttatg ctgccctttt cattgcgaat ggaacgcgaa
     2641 caaacacgat ctctttatag cagcggcagt ttggcagctc gcccgcgtgg ccggtcatgc
     2701 cgtccaaagt tggaggtgaa tcccagcgga cgaatttacc tttcatgtga tcgtgggaat
     2761 ctcggacatc gccatcatcg gctgtacgcc aaatataacc atccgagcca atagctatcg
     2821 ctcgggccat atccagcgcg cccgttgctc ttcccagttc cgttcgggca atcagatcgg
     2881 ccctagactt agcaacatcg ccggtagatg caatctcttt agcaaactgc tccgcccgac
     2941 caccggtgac aacagcctct atcgctttgt tctggatgtc gtacaccctg tctgcggcct
     3001 cgagaggcag agatttgatg tacttgacct gttcggcgat gatggattgc atcacctggc
     3061 ccacaggagc gctttccaca agattgcgga gctcgcgact gatgttcttg ctgtgttgcc
     3121 gccaaacttt ctcgttctgc cgggttaggt ccgcagtaaa gttttccgcg acctttgtcg
     3181 cccagggggt gatgatttca ctgtaacgct ccagcgccgc aataatttcc gtgatactgt
     3241 catttgaacc atcgtagcga ccatttacga tgtctccgac cgccctcgct atcctgcgta
     3301 ggctggttcg ataccggatt tccgcctgac ggctcctgcg gttcgtcatc aggttcgccg
     3361 atgccgggcg gcgcttcatc ttcggcattc tcgatgtcct cgttggtaat ggatgcccct
     3421 atgccggtta cgtcagaatt ttctcgcaaa tcagtcatag cggctttcag tgtcatcaga
     3481 ccatcaccca gcgccgtact gattgcgttg gtggtgttta acgccaccgt tgagcgatcg
     3541 acatcagaca tttgccagag cgggttaaac tcaaacgtga aatcgtccgg cagcggcttg
     3601 ccaagttccg aacgatgcat gatgtccagt atccgccgca ccggaagacg taaacgcctc
     3661 tcctgcaacg aactgatgcg atcgtaatag ttggcaaggt ctgcatcacc ggtagaaaat
     3721 cctttcgggg actgtccgaa caaccgcacc agtgggatac caacagcgcc actaatctgt
     3781 tctgcaaact gcgaaaggat gtcatccaga ccactgaagc tgtactgatg ggtttcaaac
     3841 ttatcccgcg agtccatgag cgtcatgcct tcattactct ggaactgtcg aatcaggtcg
     3901 atattcttca gcaacgcttc atacgcagga ccaccaagtg cgataagctc gcgtagcttc
     3961 tccacgctgt aggtacgcaa atgcgccttg tagaccagct gcgccgcgcc gacagtagcg
     4021 ctgtcgaacg cggtaagacg atcccagata cgctctacaa ccgacattcc ccattcgttc
     4081 tcggtcatct tctgctgaaa tggcaacgtg acgccatcaa agcgaatcag gcgactgtga
     4141 tgaatgcgcc aggcaggaat tcccgttgct gtggtcacca catcgtaaaa ctcaggttta
     4201 cccaggtccg gccccatatc tttaatgcgg cgggtcagtg ccgggtcgac catccagcgg
     4261 tcgagcggga gaatcccctt aaacttgccc ttaccgatgg tttcgggtcg cagcggggtc
     4321 attggtgcct gcccctcaat catgatgaaa cccaccgcgc cgccgtagag gcgcgaccat
     4381 ttcagcacgt cattcagcgc atcccagatt tgcaactcat ccagttgtga ttcgagaatg
     4441 ccacgatctt ttgcatcaat ttccgaagtg atgcgaatgc ctttgcgggt catatcatcc
     4501 gggatagcat cgactgcttc gccgatgatc caggatgaac gataggacca ttccaccagc
     4561 atgcggttac gactggtgaa attagcccgg taggtggatg ctgagtgctg gttaggtgtc
     4621 tgcatcccta ctcgggcaat aaaattctca taaccatcag ctgtggcctg cacagttcgc
     4681 cgcagggctt gtttgtttcg tgccatcagg cctgtctccc cagctgttcc cagatatcca
     4741 gcgatgtatc gattggcgcg aaggccataa tgaatgcatc agcaacgttt ggtgacggta
     4801 tctcgcgttt tgcgaggtct tttttacttt cgaccatcac acgtccgtta cggtcgaaat
     4861 cacgatgagg tgttgtcagt tccagtttca gcttttcaag caacggacaa cgagaatcta
     4921 tgctgatcag ctcatccaca ggatactgtt ctccgttgtt aatggcgtta aacgtatttc
     4981 tgaaacggtc agccaccatg cctgagcctt aagatttgcg aaaaagtctt tgttggggat
     5041 gccgttgtac tcgtcatctg gctcatgcac accagcaccg gcgttaaacc tctggtaatt
     5101 cacacgtcgc gcgtatgcgt tctcgctctt ccggtcagcg ttaatttcag agaatttagc
     5161 accggcagac gcaccaacac cgatagagtc gtaaacaata tctgcttcac gctccagcgc
     5221 cgcctgataa gtacgctggc agctcttcag taattcatct tctttggcct tccattcgtc
     5281 ggcccagaaa acaacggatc cgt
//
LOCUS       DAJTPG010000174         5277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTPG010000174 DAJTPG010000000
VERSION     DAJTPG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            112..1170
                     /gene="rsxD"
                     /locus_tag="OO230_003837"
     CDS             112..1170
                     /gene="rsxD"
                     /locus_tag="OO230_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5693406.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1174..1794
                     /gene="rsxG"
                     /locus_tag="OO230_003838"
     CDS             1174..1794
                     /gene="rsxG"
                     /locus_tag="OO230_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5693407.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1798..2493
                     /gene="rsxE"
                     /locus_tag="OO230_003839"
     CDS             1798..2493
                     /gene="rsxE"
                     /locus_tag="OO230_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5693408.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            2493..3128
                     /gene="nth"
                     /locus_tag="OO230_003840"
     CDS             2493..3128
                     /gene="nth"
                     /locus_tag="OO230_003840"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5693409.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            3739..5241
                     /gene="dtpA"
                     /locus_tag="OO230_003841"
     CDS             3739..5241
                     /gene="dtpA"
                     /locus_tag="OO230_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5693410.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
BASE COUNT         1250 a         1293 c         1347 g         1387 t
ORIGIN      
        1 aatgcggaac cagaagaaca ggtcgatccg cgcaaagccg cagttgccgc ggctattgcc
       61 cgcgttcagg ccaaaaaagc cgcccagcaa aaggttgtaa acgaggacta aatggtattc
      121 agaatagcta gctcccctta tacccataac cagcgccaga catcgcgcat tatgctgttg
      181 gtgttgctcg cagccgtgcc aggaatcgca gcgcaactgc ggttttttgg ttggggtact
      241 ctcgttcaga tcctgttggc gtcggtcagt gctctgttag ccgaagctct cgtactcaaa
      301 ctacgcaagc agtcggtagc cgcaacgttg aaagataact cagcattgct gacaggctta
      361 ttgctggcgg taagtattcc ccccctagcg ccatggtgga tggtcgtact gggtacggtg
      421 tttgcggtga tcatcgctaa acagttgtat ggcggtctgg ggcaaaaccc gtttaatccg
      481 gcaatgattg gttatgtggt cttactgatc tccttcccgg tgcagatgac cagctggtta
      541 ccgccacatg aaattgcggt caacatccct ggttttatcg acgccataca ggttattttc
      601 agcggtcata ccgccagtgg tggtgatatg aatacgctac gcttaggtat tgatggcatt
      661 agtcaggcga caccgctgga tacattcaaa acctctgtcc gcgcaggtca ttcggttgaa
      721 cagattatgc aatatccgat ctacagcggt attctggcgg gcgctggttg gcaatgggta
      781 aatctcgcct ggctggctgg tggcgtgtgg ttgctatggc agaaagcgat tcgctggcat
      841 attcccctca gcttcttagt aacgctggcg ttatgcgcaa cgttgggctg gttgttctca
      901 ccagaaacac tggcagcacc gcaaattcat ctgctgtctg gtgcgactat gctcggcgca
      961 ttctttattt tgactgaccc agttactgct tctacgacca atcgtggtcg gctgatgttc
     1021 ggcgcgctgg cgggcttatt agtctggttg atccgcagtt tcggcggcta tcctgacggc
     1081 gtggcttttg ccgtcctgct ggcgaacatc acggttcctc tgatcgatta ctacacgcgt
     1141 ccgcgcgtct acggccatcg caaagggtaa accatgctga aaactatccg aaaacacggc
     1201 attacactgg cactatttgc agcgggttct acagggttaa ctgcggccat caaccagatg
     1261 accaaaacga caattgctga acaggccagt ctgcaacaaa agacgttatt tgatcaggtg
     1321 ctgccagccg aacgctataa caatgcgctc gcacagagtt gctatctggt aactgcgcca
     1381 gagttaggta aaggtgagca tcgggtttac atcgccaaac aggatgacaa accggtagcc
     1441 gccgttctgg aagcaaccgc gccagatggc tattccggtg cgattcagct gctggtggga
     1501 gccgatttta atggcacggt acttggcacg cgcgtgacag agcaccacga aacgccaggg
     1561 cttggcgata aaatcgaatt gcgcctttct gactggctca cccattttgc gggaaaaaaa
     1621 atcagtggtg cggatgatgc gcactgggcg gtgaagaaag atggtggtga tttcgaccag
     1681 ttcaccggcg cgacgattac tccccgcgcg gtggttaatg cggtaaaacg cgccggattg
     1741 tacgctcaga cgttaccggc acaactttct caacttcctg cctgtggaga ataaagcgtg
     1801 agcgaaatta aagacgttat tgttcagggg ttgtggaaaa acaactctgc actggtccaa
     1861 ctgctcggcc tttgtccgct attggcggtc acatccactg ccactaacgc tctgggttta
     1921 ggacttgcga ctacgctggt actgacgctg accaacctga ccatttcgac gctgcgtcac
     1981 tggacgccag ccgagatccg cattcccatt tacgtgatga tcatcgcctc ggtggtcagc
     2041 gctgtacaga tgctgatcaa cgcctacgcc tttggcctgt atcaatcatt agggattttt
     2101 attccgctga ttgtcactaa ctgtatcgtt gtgggccgcg ctgaagcctt cgccgccaaa
     2161 aaaggtccgg cgctttcggc actggacggc ttttcaattg gtacgggcgc aacctgcgcc
     2221 atgttcgtgc tgggttcact acgcgaaatt atcggcaatg gcacgttgtt tgacggtgca
     2281 gatgcgctgt taggtagctg ggcaaaagta ttacgcgtgg agattttcca caccgactcc
     2341 cctttcctgc tggcgatgct gccaccaggt gcatttattg gcttgggact gatgctggca
     2401 ggaaaatacc tgattgatga aagaatgaaa aagcgccgtg ctgaagcagc tgccgaacgt
     2461 gcattgccaa acggtgaaac agggaatgtc tgatgaacaa agcaaaacgt ctggagatcc
     2521 tcactcgcct gcgtgagaac aatcctcatc ccaccaccga gcttaatttc agttcgcctt
     2581 ttgaattgct gattgccgta ctgctttccg ctcaggcgac cgatatcagt gttaataagg
     2641 cgacggcgaa actctacccg gtggcgaata cgcctgcagc gatgcttgaa ctaggcgttg
     2701 aaggggtgaa aacctatatc aaaacgattg gcctttataa cagcaaagca gaaaatatca
     2761 tcaaaacctg ccgtatatta ctggaacaac ataatggcga ggttccggaa gatcgtgctg
     2821 cgcttgaagc cctgcccggc gtaggtcgta aaacagccaa cgtcgtatta aacactgcat
     2881 tcggctggcc gactattgct gtcgacacgc acattttccg cgtttgtaat cgtactcaat
     2941 ttgcgccggg gaaaaacgtc gaacaggtag aagaaaagct actgaaagtg gttccggcag
     3001 agtttaaagt cgactgccac cattggttga tcctgcacgg gcgttatacc tgcattgccc
     3061 gcaagccccg ctgtggctct tgtattattg aagatctttg tgaatacaaa gagaaagttg
     3121 acatctgaag aaaaggggta acaccgatta ccccattgat aacctttctt tatcctcttt
     3181 taaaacatct ttttaacgtc aatgatgcca ttgcttagcg ttatcatcag gtaatccgtt
     3241 tgcggataac caaaaatgca ggttaattgt ttttttaata gcgaaatttt ctattcattc
     3301 gtgatcaaga tcacgatgct gtggcaatgt aaaaacattg caagaatcag gttaaaatga
     3361 tttaaattta cacacgcaac aaatattgac ctgcaaaaca ttacactggc tatttttcag
     3421 aaactggact atctcactag gaaaaacgct aaatagcaga acatatcgcc gccaagccat
     3481 catccccctc tcaatgcagt gaaaaaatct gccgttacgt tttttgaaaa atttaacgct
     3541 ggataacatt tcccggaatg gttgaattcc ccgcctcagt tatatgtaac agattattac
     3601 aaaggacttg tctgaaagtg caagatagtg aacattacct gccgtttccc ctcccactat
     3661 aacaattgcg cgtatgtttt ttagacataa cgcgagaaag cacccccgtt aatatgggat
     3721 gtaaaaaaag aggtaaaagt gtccactgca aaccaaaaac caactgaaag cgtcagtttg
     3781 aacgctttca aacaaccgaa ggcgttctat ctcatcttct cgattgagtt atgggaacgt
     3841 tttggttatt acggcctaca aggaattatg gctgtttacc tggttaaaca actgggtatg
     3901 tctgaagcgg attcaatcac ccttttctct tcctttagtg ccctggttta tggtctggtc
     3961 gctatcggcg gctggttagg tgacaaggta ctgggtacta aacgcgtaat tatgctcggc
     4021 gctattgtgc tggcgattgg ttatgctctg gttgcctggt ctggtcacga cgccggtatt
     4081 gtttatatgg gtatggcggc tattgcggtc ggtaacggcc tgtttaaagc taacccgtct
     4141 tctctgcttt ctacatgcta tgagaaaaac gacccacgtc tggacggtgc attcaccatg
     4201 tactacatgt ccgtcaacat cggctctttc ttctctatga tagccacccc gtggctggcc
     4261 gcgaaatacg gctggagtgt tgcgtttgcg ttgagcgttg taggcctgct gatcactatc
     4321 gttaacttcg ccttctgcca acgctgggtt aaacagtacg gttcaaaacc agactttgaa
     4381 ccagtcaact accgtaacct gttgctgacc attattggtg ttgtggcgct gatcgctgtt
     4441 gccacctggc tactgcacaa tcaggaagtt gcgcgtatgg cgctgggcgt tgttgccttc
     4501 ggtattgtgg ttatcttcgg taaagaagct ttcgcgatga aaggtgctgc gcgtcgtaaa
     4561 atgatcgttg ccttcatcct gatgctcgaa gcaatcatct tcttcgtgct gtacagccag
     4621 atgccaacgt cactgaactt ctttgcgatt cgtaacgttg agcactccat tctgggtctg
     4681 gccgtagaac ctgagcagta tcaggcactg aacccgttct ggatcatcat cggtagtccg
     4741 attctggccg ctatctataa caagatgggt gataccctgc cgatgccaac caagtttgca
     4801 atcggcatgg tgatgtgttc tggtgcgttc ctgattctgc cgctgggtgc gaaattcgcg
     4861 tctgacgcag gtatcgtgtc tgtcagctgg ctggtcgcaa gctatggcct gcagagcatc
     4921 ggggaactga tgatctctgg tctgggtctg gcaatggttg cacaactcgt tccgcagcgt
     4981 ctgatgggct tcattatggg tagctggttc ctgaccactg ccggtgcaaa cctgattggt
     5041 ggttatgttg cgggtatgat ggctgtgccg gataacgtta ccgacccgct gatgtcactg
     5101 gaagtctatg gtcgcgtatt cttgcagatt ggtgtcgcta ctgccgttat tgcagtactg
     5161 atgctgctga ccgcgcctaa actgcaccgc atgacgcagg atgacgctgc agacaaagcg
     5221 gcgaaagcta ctgtagcgta aaattcaggg aaactctttt acaagccgct aactttt
//
LOCUS       DAJTPG010000175         5266 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTPG010000175 DAJTPG010000000
VERSION     DAJTPG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..375
                     /locus_tag="OO230_003842"
     CDS             <1..375
                     /locus_tag="OO230_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5693411.1"
                     /translation="RLHLIANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWV
                     AKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQP
                     EGEHSAHEGMEGMDMSHAESAH"
     gene            397..2388
                     /gene="cyoB"
                     /locus_tag="OO230_003843"
                     /pseudo
     CDS             397..2388
                     /gene="cyoB"
                     /locus_tag="OO230_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2378..2992
                     /locus_tag="OO230_003844"
     CDS             2378..2992
                     /locus_tag="OO230_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5693412.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            2992..3321
                     /locus_tag="OO230_003845"
     CDS             2992..3321
                     /locus_tag="OO230_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5693413.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3333..4223
                     /gene="cyoE"
                     /locus_tag="OO230_003846"
     CDS             3333..4223
                     /gene="cyoE"
                     /locus_tag="OO230_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5693414.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4407..4718
                     /locus_tag="OO230_003847"
     CDS             4407..4718
                     /locus_tag="OO230_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5693415.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4853..5251
                     /locus_tag="OO230_003848"
     CDS             4853..5251
                     /locus_tag="OO230_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5693416.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1150 a         1304 c         1393 g         1419 t
ORIGIN      
        1 cgcctgcatc tgatcgccaa cgaacccggt acttatgacg gtatctccgc cagctacagc
       61 gggccgggct tctcaggcat gaagttcaaa gctattgcaa caccggatcg cgccgaattc
      121 gaccaatggg tcgcaaaagc gaaacagtcg ccgaacacca tgtctgacat ggctgcgttc
      181 gaaaaactgg ccgcgcctag cgaatacaac caggtggaat atttctccaa cgtgaaacca
      241 gacttgtttg ccgatgtgat taacaagttt atggctcacg gtaagagcat ggacatgacc
      301 cagccagaag gtgagcacag tgcacacgaa ggtatggaag gcatggacat gagccacgcg
      361 gaatccgccc attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat
      421 gcagtcccgt tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt
      481 ctggcgctcg ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg
      541 ctgacctccg tcgaccataa acgcctcggt atcatgtata tcatcgtggc gattgtgatg
      601 ttgctgcgtg gttttgctga cgccattatg atgcgtagcc agcaggctct tgcctcggcg
      661 ggtgaagcgg gcttcctgcc acctcaccac tacgatcaga tcttcaccgc gcacggcgtg
      721 attatgatct tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg
      781 ctgcagatcg gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt
      841 actgttgttg gcgtgattct ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc
      901 ggctggctgg cctatccacc gctatcggga atagagtaca gtccgggagt cggtgttgat
      961 tactggatat ggagtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc
     1021 ttcgttacca ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat accagtattt
     1081 acctaggcat cactgtgcgc gaacgtactg attattgctt ccttcccaat tctgacggtt
     1141 accgtcgcgt tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg
     1201 ggtggcaaca tgatgatgta catcaacctg atttgggcct ggggccaccc ggaagtttac
     1261 atcctgatcc tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa
     1321 cgtctgtttg gttatacctc gctggtatgg gcaaccgtct gcatcaccgt gctgtcgttc
     1381 atcgtttggc tgcaccactt ctttacgatg ggtgcgggcg cgaacgtaaa cgccttcttt
     1441 ggtatcacca caatgattat cgccatcccg accggggtga agatcttcaa ctggctgttc
     1501 accatgtatc agggccgcat cgtgttccat tctgcgatgc tgtggactat cggttttatc
     1561 gtcaccttct cggtgggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc
     1621 gttctgcata acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg
     1681 gtcttcggct gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg
     1741 aacgaaacct ggggtaaacg cgcgttctgg ttctggatca tcggcttctt cgttgccttt
     1801 atgccgctgt atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac
     1861 ccgcagttcc acaccatgct gatgattgca gccagcggtg cagtactgat tgcgctgggt
     1921 attctctgcc tcgttattca gatgtacgtt tctattcgcg accgcgacca gaaccgtgac
     1981 ctgactggcg acccgtgggg tggccgtacg ctggagtggg caacctcttc cccgcctccg
     2041 ttctataact ttgccgtagt gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa
     2101 gagaaaggcg aagcgtataa aaagcctgac cactatgaag aaattcatat gccgaaaaac
     2161 agcggtgcag gtatcgtcat tgcagctttc tccaccatct tcggtttcgc catgatctgg
     2221 catatctggt ggctggcgat tgttggcttc gcaggcatga tcatcacctg gatcgtgaaa
     2281 agcttcgacg aggacgtgga ttactacgtg ccggtggcag aaatcgaaaa actggaaaac
     2341 cagcatttcg atgagattac taaggcaggg ctgaaaaatg gcaactgata ctttgacgca
     2401 cgcgactgcc cacgcgcacg aacacgggca ccacgatgca ggcggaacca aaatcttcgg
     2461 attttggatc tacctgatga gcgactgcat tctgttctca atcttgtttg ctacctatgc
     2521 cgttctggtg aacggcaccg caggcggccc gacaggtaag gacattttcg aactgccgtt
     2581 cgttctggtt gaaactttct tgctgttgtt cagctccatc acctacggca tggcggctat
     2641 cgccatgtac aaaaacaaca aaagccaggt tatctcctgg ctggcgttga cctggttgtt
     2701 tggtgccgga tttatcggga tggaaatcta tgaattccat cacctgattg ttaacggcat
     2761 ggggccggat cgcagcggct tcctgtcagc gttctttgcg ctggtcggca cgcacggtct
     2821 gcacgtcact tctggtctta tctggatggc ggtgctgatg gtgcaaatcg cccgtcgcgg
     2881 cctgaccagc actaaccgta cccgcatcat gtgcctgagc ctgttctggc acttcctgga
     2941 tgtggtttgg atctgtgtgt tcactgttgt ttatctgatg ggggcgatgt aatgagtcat
     3001 tctaccgatc acagcggcgc gtcccatggc agcgtaaaaa cctacatgac aggctttatc
     3061 ctgtcgatca ttctgacggt gattccgttc tggatggtga tgacaggggc tgcctctccg
     3121 gccgtaattc tgggaacaat cctggcaatg gcagtggtac aggttctggt gcatctggtg
     3181 tgcttcctgc acatgaatac caaatcagat gaaggctgga acatgacggc gtttgtcttc
     3241 accgtgctaa tcatcgctat cctggttgta ggctccatct ggattatgtg gaacctcaac
     3301 tacaacatga tgatgcacta agagcggcgg ttatgatgtt taagcaatac ctgcaagtaa
     3361 cgaaaccagg catcatcttt ggcaacctga tctcggtgat tgggggattc ctgctggcct
     3421 caaagggcag cattgattat cccctgttta tctacacgct ggttggggtg tcactggttg
     3481 tggcgtcggg ttgtgtgttt aacaactaca tcgacaggga tatcgacaga aagatggaaa
     3541 ggacgaagaa tcgggtgctg gttaaaggcc tgatctctcc tgctgtctcg ctggtgtacg
     3601 ccaccttgct gggtattgct ggctttatgc tgctgtggtt tggcgcgaat ccgctggcct
     3661 gctggctggg ggtgatgggc tttgtggttt atgtcggcgt ctatagcctg tacatgaaac
     3721 gccactcagt ctacggcaca ttgattggtt cgctctccgg cgctgcgccg tcggtgattg
     3781 gctactgtgc agtaaccggc gagtttgata gcggcgcagc gatcctgctg gctatcttca
     3841 gcctgtggca gatgcctcac tcctatgcca tcgccatttt ccgctttaag gattaccagg
     3901 cggcaaacat tccggtattg ccagtggtaa aaggcatttc ggtggcgaag aatcacatca
     3961 cgctgtatat catcgccttt gccgttgcca cgctgatgct ctctcttggc ggttacgctg
     4021 ggtataaata tctggtggtc gccgcggcgg ttagcgtctg gtggttaggt atggctctgc
     4081 gcggttataa agttgctgat gacagaatct gggcgcgcaa gctgttcggc ttctctatca
     4141 tcgccatcac tgccctctcg gtgatgatgt ccgttgattt tatggtaccg gactcgcata
     4201 cgctgctggc tgctgtgtgg taacaaaacc tctctattaa aaaggtgcta cggcaccttt
     4261 ttattgtctg agatcagaga tacggctaat aaccgtatct cttgacagag tgagcagatg
     4321 cacataaatt aatcatacgg ttgatagccg tattatgtat gagatcgact ccctacgatt
     4381 tcattcagtt aatcaaagaa gtcataatgc ctggaaaaac agccgctcta tacgatgtcg
     4441 ataaaacgct gaaaaatgcg cgggtcgaac tcaaaactag tcccgacgcc aaaaacaaac
     4501 ttcgtgaagc tgcgcaagcc gttggggtag atttaagcgc ttttattttg agcgctgcta
     4561 tggaacgcgc cgaaagtgtg cttgataacc aacgtcgccg tgagctttcg aatcaaagct
     4621 gggaactgat gaaccaactc atcgctgaac ctgctcaacc gacgctcgcc ctcaaggcgt
     4681 taatgaaaag gaaaaacagc gatggtcgac aagcatgaag agattactct gcccatagtc
     4741 ctctcctgta attatcagtc tgatattact tatcctgggc aaaaacagtt tgattgcggt
     4801 aaccctgtta tcgataaatt tgtacgcgct acgcgcatca ctaaagaaaa gtgtgcgtaa
     4861 tagcgactgt gcggctaaag cacttattga cagacaaagt ggtgaactga ttggcatctg
     4921 tacttttacg gcatattcgc tggaaagaca acgcgtttct ggcgtccttc agggttcaca
     4981 accttcagaa attggtgttg tcagattagt catgttgggg ggagcgcgga agtatcaaaa
     5041 gcggggcttt ggtcaggacc tactatgtga tttttttgaa catgtaaaaa aaattcaccc
     5101 ggcattacca attaaagggg tttatcttga tgctgaccct gccgccatta acttttatgc
     5161 tcgtctcggc tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt
     5221 tttggcgatt cagcatattc tcgcggctta gattattatc gccacc
//
LOCUS       DAJTPG010000176         5263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTPG010000176 DAJTPG010000000
VERSION     DAJTPG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1631)
                     /locus_tag="OO230_003849"
     CDS             complement(<1..1631)
                     /locus_tag="OO230_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5693417.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWV"
     gene            complement(1647..2531)
                     /locus_tag="OO230_003850"
     CDS             complement(1647..2531)
                     /locus_tag="OO230_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5693418.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2542..3147)
                     /locus_tag="OO230_003851"
     CDS             complement(2542..3147)
                     /locus_tag="OO230_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5693419.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3318..>5263)
                     /locus_tag="OO230_003852"
     CDS             complement(3318..>5263)
                     /locus_tag="OO230_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR5693420.1"
                     /translation="VTDSPLLITGSINASGLVFIDSYYGNPSTIKGSINARGVFINDI
                     IAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIFKRSNI
                     RMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMRTLPRL
                     KGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDREPLSVE
                     IMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPESIIDGY
                     IRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRMTPQES
                     DKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVIHSDAA
                     LQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETSADMIL
                     SELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPRNDIWA
                     KFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFG
                     ALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPELDLVR
                     QLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1514 a         1316 c         1101 g         1332 t
ORIGIN      
        1 acccacggtt taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg
       61 tgccacggct gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggctagacct
      121 gcgagtgagt tttttacctg ctcattttcg ggaaaaagct catataggcc agagacaatc
      181 gccagcgtag gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt
      241 tcaaaacgca cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca
      301 tttaactcac tgtaatcacg taacaaaata tcgcttacgg caggctcgtt tgccagcgca
      361 tccagtacat agcgtccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg
      421 gcgtggagat cggcaacggc ttgtttaatc aatatttgca aatgggtttt gcgctggcga
      481 ataccgcgcc atccaacact gttcaggtag attttgtcga ttaaacgccc gaatgcgtta
      541 ctgccttgcg gttgattttg atagacataa tccagggaac tgcctgaatc aaagccggta
      601 ctcattccga gatgcaggcc cgcagagtgc gcaccgaaca atttcatcgc ctttcgcata
      661 aagcgatacg ctaaatcaac cggcgataat ggcacgggtc caccagaaag gagccgccat
      721 cgatccgctg atggtcctgt gcggtcttca tgctgataat caaatttttg cgatttgtta
      781 gcatataagc ggcagataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca
      841 cccaatgtgt cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat
      901 ctctgataaa aatcaatctg cggttggcga tgcaccacat agtcatcacc tgatatcaga
      961 agttgcgtgg ggagcgtaat cgccgccgca tcgctaacaa tacgttcaga cgttttatag
     1021 agatcgagca agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc
     1081 gccacccgtt gccgatcgtg ggtcaaatag cgccctttca cataggaatt aataaaaaat
     1141 agaccacgca gacgatgcca taacgccagc gcaggacgtg ccagcggcac atacaattta
     1201 accttaaagg ccggagaagc cagcaccagc ccacgaattg caggtgcata atcatgaacc
     1261 catgtggcaa caagcactgc gccgacgctt tgcgcgatca ctaccacctc ttccagtccg
     1321 acctggctgt cgctggcagc aaaacggaca aattcatcga catcctgcac tgagcgcata
     1381 agagacgggc tataaccacg cggccccgaa ttttttccat gcccccgggc atcccaggca
     1441 taaaaagcag tatcaggcat cgccagttca tcaacgagat gttgtagacg accagaatgt
     1501 tcatgcccgc gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agtgggccag
     1561 tgccgataat acaatgctgt attatcactg gtggtaaaaa aatgttccct agggatgcgt
     1621 gaattttcca ttttctctat tttccttcag taacagcagt agcgaataaa ataaaaaaat
     1681 accggagcgg tgaaaattaa tgagtcaatc ctgtcaagga gtccgccatg tcctagtaat
     1741 agttttccac tatctttaac accaatatct cgtttgatgg ctgacatcac tacatcgccg
     1801 caaaaaccac taataccaat taacaaaccc gctaataatg cctgtaatgt atttagcggt
     1861 gtcagcaacg gtccaataat cagtgacgca atcatggtgg tgattacgcc ccccagcaga
     1921 ccttccagcg tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc
     1981 ccccataaat actgtgcaat atcgtttgac tcggttaacg ccaaaagaaa taacaccagt
     2041 aacgccccgc cctggatatt tatggttggc aacattaata gccaggcggc atgactcaag
     2101 gcaaaaacgg tcattatcca tccccaaaaa atggcgctca cggtatgctg aaaaccggag
     2161 gggtcaccca caaaaactcg ccaggtggct aatatcagaa acccagtcaa aggtataaat
     2221 aacagaaaca gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa
     2281 tataaccaac gcgaaaaatg tacggatata agcgtacaat attctttcaa tgccagaaaa
     2341 cttatcagcg taaaaaacgt caacgtcatc cagcgtgggc ctgaaatcac cagtgaaaaa
     2401 caaatgacga tgccccacca ggtacgaata cgtaatgtaa cttcatcccc ttttctctca
     2461 ggtaaccgcc atagcagaaa acggtttatc agcttgctaa aattaacaac gcgaaaagtg
     2521 ttgccagaga tttttccagc attatctttc agcgctcata agaagcacac tccgacaacg
     2581 attaatcgca gtacagataa gcagaattga agcaatgctc cataaaagat tattccattg
     2641 catccactgt ggataaatgg caacggccag accccacaga ccaaatatta acgcgcgatc
     2701 acttttgcca aatggaccgg cataactgcg aacaccgtta atcgtctgcg cgagtaaacc
     2761 gcaaaactcg gtcaatatgg tgcaaaataa catgagtatc acgagtgatg cgttactttc
     2821 cggtaaaaat aaaaacggta aatagagcgc aatatcggaa ataacatcgc cagtctcatt
     2881 caaaatcgcc cccagacgtg tttgctgatt gcactcacgc gccaacatgc catccagcgc
     2941 attgagcgcc atacggataa aaagcacgat gggcaatagc aaaaagagga tgggttgtgc
     3001 caccaacacc aacagcaatc cggtaaaaag agaaagcgcc agtgccgtaa gagtgatgtg
     3061 attcgctgta acgtggtgct tataaagcca aaacatcgtc ggccttaaca gcgactgaaa
     3121 gagcggtttt atctggtaga gcgtcattat caatccttga taattcggtt tgcgctatgg
     3181 tgcatcaatt cccctgacgg agatatcgca acacgccgct tcattaaaaa ttcagaatat
     3241 atcaaggtat tgggggctca ctgtcaggaa tgattatccc caataaatca agtagttaaa
     3301 aattattacg ctgttattca atacaactca tcaagtttcc tcatcgcttg ttcccgtatt
     3361 gcgcgcgtct taccgttaag cgccagctgg cggactaaat ccagttcagg gttaacaagc
     3421 tctaacgcca gcaaaggatc acgtttcagc ctcgccttca atacatcaac tggaaagcgg
     3481 ggattattca tcgccacaat ccaccactcg ggatcaattt ctgccgccag tgccgcagag
     3541 ggggtgtggc ggttgctaag tgcgccgaac ttgaaaaatg tgagattgct tttcgccagt
     3601 gtactccacc atatacgatc cggttgtttt gccagtacat ccagttgtgc taacgcatcg
     3661 gggtcgagtg cataataaac acgtaatgct tcatcaacct gcgcacggga gctattttcc
     3721 tgcctcagaa aaaacttcgc ccaaatatca ttgcgtggcg tgttttgctg taattccttg
     3781 agaacaacag cgggtaaatt atcccaacta actaatgcac tctctaaaaa gtcccagtct
     3841 tccaaacgta tcagggcggc ggcatttaat agcatctggt catcaacttc gccgtggcta
     3901 atcagctcag acaaaatcat atcagcgctg gtttcatatg catctgaatc tgctttcgga
     3961 ttatttttcc gatgcgataa agcacgttgc caaatttttt gtttcatcga cgtgctcaga
     4021 tactgatttt tcgccagatt actccacccc tcaggatcgt tgaaacgtgt caataaccgt
     4081 tgtaaagcag cgtcagagtg aattacctgc atatcccgac agcaaaagta ggcaggggat
     4141 tttttctcca tccttttaac aaaattaaac tgtaagttat ctggcaatgc taaaaataac
     4201 acacgtatta gatcatcgtc attctcgaat ttttggtata aataggctgc aactttgtca
     4261 ctctcttgtg gcgtcatccg caataattga ccatggtgtt tcatctctgc cagcttgaac
     4321 gctaagcttg tgacaacatt gtttgatgaa tcgttcgcca actgcattgc ctgattgtaa
     4381 gtgagttgtc ctccagcacc gacctgacga atagaggaat catcactgcg gatatagcca
     4441 tcaataatgg attcgggcaa taaccacgca tctttataaa gctcctgaat gatcatatcc
     4501 caatctcttt tcgccagagt catgacgata tccagaggga gattttcacg tagcgcaaga
     4561 ttcaacggac cgacaccatc aggtagacga ctcattcgcg tcattatttc gacagagagc
     4621 ggttcacggt cgagcaaccc cagaacaaca ggctggtaac ccaattgctg atcggtcaac
     4681 tccagaatct cattgtcagg catatatctc gccatacttt caatcgtttg ttcatcattg
     4741 ctattaataa gttgtttgcg taggttagct gaaattcctt taagccttgg gagagttcgc
     4801 attatggcaa gatcaaatgt cgcaatatct cgtccttcaa atgcagcaac gttgccttca
     4861 tccagcattt gcaattcctg cactaactca ggagaaggct tagggcgaaa cagcgcttct
     4921 tttttcatct gattacagac attccctgcc atgcgaatat tgcttcgctt aaaaatttct
     4981 ttcagagcat cttcatcatt tattagaccc caataatatg catcaggatt tattatcatc
     5041 agcgcttttt tgacattttc agtgtcattt ttgtcactcg cacggaccat gaattcacta
     5101 tttgtcgaag acgcaacaac aggtgcaatt atgtcattga taaatacccc acgcgcatta
     5161 atactcccct taatcgtaga cggattaccg taatatgagt cgataaatac cagtccactt
     5221 gcatttatcg aaccggtaat taacagtggt gaatccgtaa cta
//
LOCUS       DAJTPG010000177         5253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTPG010000177 DAJTPG010000000
VERSION     DAJTPG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..944
                     /gene="tauA"
                     /locus_tag="OO230_003853"
     CDS             <1..944
                     /gene="tauA"
                     /locus_tag="OO230_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5693421.1"
                     /translation="TLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGA
                     SVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALV
                     VKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQR
                     GDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFA
                     KSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELT
                     GPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            957..1724
                     /gene="tauB"
                     /locus_tag="OO230_003854"
     CDS             957..1724
                     /gene="tauB"
                     /locus_tag="OO230_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5693422.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1721..2548
                     /gene="tauC"
                     /locus_tag="OO230_003855"
     CDS             1721..2548
                     /gene="tauC"
                     /locus_tag="OO230_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5693423.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2545..3396
                     /gene="tauD"
                     /locus_tag="OO230_003856"
     CDS             2545..3396
                     /gene="tauD"
                     /locus_tag="OO230_003856"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5693424.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3502..4476)
                     /gene="hemB"
                     /locus_tag="OO230_003857"
     CDS             complement(3502..4476)
                     /gene="hemB"
                     /locus_tag="OO230_003857"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5693425.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            4999..>5253
                     /locus_tag="OO230_003858"
     CDS             4999..>5253
                     /locus_tag="OO230_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693426.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGV"
BASE COUNT         1211 a         1321 c         1468 g         1253 t
ORIGIN      
        1 acacacttct tgccgcactg gcattcatcg cttttcaggc acaggcggtg aacgtcaccg
       61 tggcgtatca aacctcagcc gaaccggcga aagtggctca ggccgacaac acctttgcta
      121 aagagagcgg ggcgagcgtg gactggcgta agtttgacag cggagccagc atcgtgcggg
      181 cgctggcttc tggcgacgtg caaatcggca acctcggttc cagcccatta gcggttgcag
      241 ccagccaaca ggtgccgatt gaagtcttct tgctggcgtc aaaactgggt aactccgaag
      301 cgctggtggt aaagaaaact atcagcaaac ccgaagattt gattggtaaa cgcattgccg
      361 taccgtttat ctccaccacc cactacagcc tgctggcggc gctgaaacac tgggggatta
      421 aaccagggct agtggagatt gtgaacctgc agccgcccgc gattatcgcc gcatggcagc
      481 ggggtgatat tgatggtgct tatgtctggg caccggcggt taacgccctg gaaaaagacg
      541 gcaaggtgct gaccgattct gaacaggtcg ggcagtgggg tgcgccaacg ctggacgtct
      601 gggtggtgcg caaagatttt gccgagaaac atcctgaggt cgtgaaagcg ttcgccaaaa
      661 gcgccatcga tgcacagcaa ccgtacattg ctaaccctga cgcgtggctg aaacagccgg
      721 aaaacatcag caaactggcg cgtttaagcg gcgtgcctga aggtgacgtt ccggggctgg
      781 tgaagggaaa tacctatctg acgccgcagc aacaaacggc agaactgacc ggaccggtga
      841 acaaagcaat catcgacacc gcgcagtttt tgaaagagca gggcaaggtc ccggctgtag
      901 cgaatgatta cagccagtac gttacctcgc gcttcgtgca ataaaaggag gcgcagatgc
      961 tgcaaatctc tcatctctat gccgattatg gcggcaaacc ggcactggaa gatatcaacc
     1021 tgacgctgga aagcggcgag ctactggtgg tgctggggcc gtccggctgc ggtaaaacca
     1081 ccctgctgaa tctgattgcc ggttttgtgc cttatcagca tggcagcatt caactggcgg
     1141 gtaagcgtat tgagggaccg ggagcagagc gtggcgtagt ttttcagaat gaagggctgc
     1201 taccgtggcg caatgtacag gacaacgtgg cgttcggcct gcaattggca ggtatagaga
     1261 aaatgcagcg actggaaatc gcgcaccaga tgctgaaaaa agtggggctg gaaggcgcag
     1321 aaaaacgcta tatctggcag ctttccgatg gtcaacgtca gcgggtgggg attgcccgcg
     1381 cgctggcggc gaatccccag ctgttattac tcgacgaacc gtttggtgcg ctggacgcct
     1441 tcacccgcga ccagatgcaa actctgctgc tgaaactctg gcaggagacg ggcaagcagg
     1501 tgctgttgat tacccacgat atagaagaag cggtgtttat ggcgactgaa ctggttctgc
     1561 tttcatccgg ccctggccgt gtgctggagc ggctgcggct caactttgct cgccgctttg
     1621 ttgcgggaga gtcgagccgc agcatcaagt ccgatccaca attcatcgcc atgcgcgaat
     1681 atgttttaag ccgcgtattt gagcaacggg aggcgttctc atgagtgtgc tcattaatga
     1741 aaaactgcat tcgcggcggc tgaaatggcg ctggccgctc tcgcgtcagg tgaccttaag
     1801 cattggcacg ttagcggttt tactcactgt atggtggacg gtggcggcgc tgcaactgat
     1861 tagcccgctg tttttgccgc cgccgcaaca agtactggca aaactgctca ccattgccgg
     1921 accgcaggga tttatggacg ccacgctgtg gcagcatctg gcagccagcc tgacgcgcat
     1981 tgtgctggcg ctactcgcgg cggtggtgat cggtattccg gtcgggatcg ccatggggct
     2041 aagcccgacg gtgcgcggca ttctggatcc ggtaatcgaa ctttatcgtc cggtgccacc
     2101 gctggcttat ttgccgctga tggtgatctg gtttggtatt ggtgaaacct cgaagatctt
     2161 actgatctat ttagcgattt ttgcgccggt ggcgatgtcg gcgctggcag gggtgaaaag
     2221 cgcgcagcag gttcgcattc gtgccgccca gtcgctgggt gccagccgtg cgcaggtgct
     2281 gtggtttgtc attttgcccg gcgcgctgcc ggaaatcctc accggattac gcattggtct
     2341 gggtgtgggc tggtctacgc tggtagcggc ggagctgatt gccgcgacac gcggtttagg
     2401 atttatggtt cagtcagcgg gtgaatttct cgcgactgac gtggtactgg cggggatcgc
     2461 ggtgattgcg attatcgcct ttcttttaga actgggtctg cgcgcgttac agcgccgtct
     2521 gacgccctgg catggagaag tacaatgagt gaacgtctga gcattacccc gctggggccg
     2581 tatatcggcg cacaaatttc gggtgccgac ctgacgcgcc cgttaagcga taatcagttt
     2641 gaacagcttt accatgcggt gctgcgccat caggtggtat ttctgcgcga tcaagctatt
     2701 acgccgcagc agcaacgcgc gctggcccag cgttttggcg aactgcatat tcaccctgtt
     2761 taccctcatg ccgaaggggt tgacgagatc atcgtgctgg atacccataa cgataatccg
     2821 ccggataacg acaactggca taccgatgtg acatttattg aaacgccacc cgcaggggcg
     2881 attctggcag ctaaagagtt accttcgacc ggcggtgata cgctctggac cagcggtatt
     2941 gcggcctatg aggcgctctc tgttcccttc cgccagctgc tgagctggct gcgtgcggag
     3001 catgatttcc gtaaatcatt cccggaatac aaataccgca aaaccgagga ggaacatcaa
     3061 cgctggcgcg aggcggtcgc gaaaaatcct ccgttgctac atccggtggt gcgaacgcat
     3121 ccggtgagcg gtaaacaggc gctgtttgtg aatgaaggct ttactacgcg aattgtcgat
     3181 gtcagcgaga aagagagcga agccttgtta ggctttttat tcgcccatat cactaaaccg
     3241 gagtttcagg tgcgttggcg ctggcaaccg aatgatattg cgatttggga taaccgcgtg
     3301 actcagcact atgccaatgc cgattacctg ccacagcggc ggattatgca tcgggcgacg
     3361 atccttgggg ataaaccgtt ttatcgggcg gggtgatacg agtgcggacg gttccctcgc
     3421 ccccttgggg agagggttag ggtgaggggg cgttcactgt actttcaaca ggttaactcc
     3481 ccctttctga gaggaaaaaa attaacgcag aatcttcttc tcagccaaat ccagcgcaaa
     3541 gtagctgaaa atcagatccg cacccgcacg cttaattgaa cctaagcttt cgagcacgac
     3601 tttctcttca tctatagcac ccgccagcgc ggcgaactta atcatcgcgt actcaccgct
     3661 cacctgatac gcgccaatcg gcaattcagt acgttcacgc agctcacgca cgatgtcgag
     3721 gtacgctcca gcaggtttaa ccatcaggca gtctgcgccc tgggcttcat ccagcagtga
     3781 ctcacgaatc gcctcgcgac ggttcattgg gttcatctga tagcttttgc ggtcgccttt
     3841 taatgcgctt cctgcagctt cacggaacgg gccataaaag gaagaggcga acttggtcga
     3901 atacgacata atcgccgtat ctttaaagcc tgcagcgtcc agcgcctgac gaatcgcctg
     3961 tacctggccg tccatcgcgg cggaaggggc gatgaagtct gcgcctgcag cagctgcaac
     4021 cacggcttgc ttgcctaaat tttccagagt cgcgtcgttg tcgacgccat gctcgcacag
     4081 cacaccgcag tgaccgtgag aagtgtattc acagaaacaa gtgtctgaca tgacgatcat
     4141 ttctggcaca gtctgcttgc agatgcgcga catacgcgcc accagtccat cttcccgcca
     4201 ggcgtcgctg ccggtttcat cggtatggtg agagatgcca aaagtcatta cggaacggat
     4261 accggcgttg gcgatgcgtt caatttcgcg tgccagatgt ttctctggaa tgcgcatcac
     4321 gcctggcatg gcttcaacgg ctttgtagtc gtcaatttct tcttcaacaa agatcggcaa
     4381 caccaggtcg ttaaggctaa gtgttgtctc ttcaaacata gcgcgcagcg caggagattt
     4441 gcgcaggcga cgagggcgtt ggattaagtc tgtcatggtc tgcctgatgt ttgtggaata
     4501 aggtgcacag tatacctgaa gcggggcagg agtgttttac gaaagttgtc gcgatgttga
     4561 caagaagaga atgaaagaga gaccaggaca tctttgtaat agaaatagca ataatttcat
     4621 attcactgaa aatattttta atctttattt atagctcttg gttattattt tttggcgttt
     4681 ggttgtgctg ctataaattg aataattcaa actttgttgt atattgataa gatatttatc
     4741 tggtttgtat ttttgatatt tataacgcgt atgcatcaaa aataacatta tctgcaacat
     4801 actgaaatat cgtcctttta tcaatttgat gcgtgttttt ctccataaat tatatatgtc
     4861 cacattcgga cttaggggaa agaataattg aaccattcgt ctgtaacgca gcataatcgt
     4921 tagcgcgaaa cataatatgt tttctatgca ttgataattg atggatcaac ttattacgtc
     4981 cctgaggagg gatgacaaat gcactcctgg aaaaagaaac ttgtagtatc acaattagca
     5041 ttggcttgca ctctggctat cacctctcag gctaatgcag cgaccaacga tatttctggt
     5101 caaacttaca atactttcca tcactacaac gacgccacct atgctgatga cgtttactat
     5161 gatggttatg taggctggaa caactatgcc gctgatagct attacaacgg cgatatctac
     5221 ccggtcatta ataacgctac cgttaacggc gtg
//
LOCUS       DAJTPG010000178         5212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTPG010000178 DAJTPG010000000
VERSION     DAJTPG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            508..1299
                     /locus_tag="OO230_003859"
     CDS             508..1299
                     /locus_tag="OO230_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693427.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1685..1891
                     /locus_tag="OO230_003860"
     CDS             1685..1891
                     /locus_tag="OO230_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5693428.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1977..2549
                     /locus_tag="OO230_003861"
     CDS             1977..2549
                     /locus_tag="OO230_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693429.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2551..2835)
                     /locus_tag="OO230_003862"
     CDS             complement(2551..2835)
                     /locus_tag="OO230_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693430.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2806..2949
                     /locus_tag="OO230_003863"
                     /pseudo
     CDS             2806..2949
                     /locus_tag="OO230_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4118..5137
                     /locus_tag="OO230_003864"
     CDS             4118..5137
                     /locus_tag="OO230_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5693431.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
BASE COUNT         1672 a         1149 c          987 g         1404 t
ORIGIN      
        1 agatttatat tcaggtgttg cgggactaca ttacagcaga gaggggtaag gacagagtta
       61 caagtactac tagagagaat atcactgcac agatacttct atgagtatat ctttatgaga
      121 gatccgtcta caggattggg ttataaggtg tatttacaag gtataatcac aggatcattt
      181 acatgaccat acttctgtaa ataatactaa ttaaccttaa ttattttttt atggtacatg
      241 aactacctgt agctgtaacg ataaacaaat cacccaaaat gaaaaatact ggttatcaca
      301 gttcagagaa ctcgtatgag tgtccccctg agcaatgctt cattttctgt gattctgtgt
      361 gatagagtta tactgagtac agtggcagta acactgcttt ccatcacttg aggataaaat
      421 tcattaaaaa attaattgca tgaattaaca ggttttttta tgaaatgtta tgcatctcaa
      481 ccaatatgat taacagaaaa cttcatcatg acaacacgta taacgttcag tatgaccagt
      541 gatgagacgc tgcgaattgt ggatgagtac tgtcataccc acaaactttc gcgctctaag
      601 gtaatcaacg cattactcag cgccacagca cctgtcctta atgatattaa ctgttattat
      661 caacttgcag gcgaactgca gtcacgctta ttaaatggag tgtatcaacg ggatcttcct
      721 cgcaaacgca atgtcgtcag tgcagagaag tattgtctgg aaatctggga aaacaaactt
      781 ttcaccagaa gaatcctgga gtttgacagt tcaaatggtg ttctgtacgc gctgaaacat
      841 aaaagacact atcgccggga taagatgatt ggaagggttg aaagtcggtg tatcaaagac
      901 atttgcgaat accagatgca gctatccggg gagaagacga aatatgcctg ttttatttat
      961 atcgaaagaa caatatacaa tcacgataat ccatctgata aaatacctgt aaaagcggct
     1021 gttggtaatg cagtcatcct gctggcaaag gatgtgatat ataacgagta cttttttgat
     1081 ttgcggcagt cattttttgt gagcgttaca gatctgatgg tctctggcgc aaaaggtatt
     1141 ccggagacac agacatatcc ggacgtttat tgctggatcc ctcttttttc cataaattcc
     1201 ggcgtgctca ttacccctgt atataaaatt gaccctctga aacccgtaac ggtaaaaaaa
     1261 ccggataaga tcactgttgt ttgtaattac agagaataat ttcagccttc taccttcacc
     1321 gcgacagtgt ttacctctgc tttatcttcc gttcagtaaa tacaaaccac cgctctggca
     1381 ggtacagggc gtggtttatc tccgatactc ccctgtgcag taataatcat cactgaaaat
     1441 actgatcccg tcacggcaaa acgccatccc atattccggc aacacaaaat catcttccgt
     1501 ttataccgga ttcactgaca aaacagacaa cctgtattgc ctgtacacaa cagtttctct
     1561 cctggcagga atattcccct actcattttt tacgtttctc cccggttgtt atgccggtga
     1621 aggtggtgcg tcgttttcat caccaacgcc ggttgagatt aacaacatcc ggaggaacat
     1681 tctcatgaat accccagttt cactgatgga tgaccagatg gtcgacatgt cgtttatcac
     1741 tcagctgacc ggcctgaccg ataagtggtt ttataaactc atcaagcatg gagcctttcc
     1801 gccccccatc aaactgggcc gtagctcccg ctggctgaaa agcgaagtgg aagcctggct
     1861 gcaggcgcgt attgcacagt cccgtccgta attcctgacc gttatccgtt ccaccgcagc
     1921 aaaggcatga tccggccccc ggaggcattt tgctgcgcgc taaaggagag aatattatgc
     1981 aacaacttga aaacaggtgt gacctgctgc tcatccagca tcagaaatgg atgacaagcg
     2041 tgacgaggct tatcgtcgct catggaatgg ggtctccgca tcttcatggt tatcaccgtc
     2101 tgaccctggc acactttttt ctgccggaaa aaggctccgt catttcggtg gccccccagg
     2161 gactctatca ggtcgtcaac ccgggaacac caccgttcat tcctgccatt caggaagggt
     2221 taatgaccag cattcagacc catgaaatca tgctgcttac acattttaat ctgggcggcg
     2281 ttttactcag tgagctacat cgtctgggtg aaaaccggct ggcaaacagg ctgaacagcc
     2341 tgctccggcg tttcgacgat cgtgatctct accacacgct gatatggctg tgctggtatg
     2401 atctgatgtg tgcccactcc atgcagccct ggactgaaga gttaaagcat aaatcacatg
     2461 cagagcttga aaactgggct gtggcgcgca aacgggaaaa gcgcgagctg gaactgatga
     2521 ttgacgagta cctgttatac gcctgctgat tcataacgtt cccggggttc cttcctcatc
     2581 gttacacaca gccggacggc tatctatggc tgtccgccaa atgaccatcg ccgcatttcg
     2641 ttccgctgaa aacggtacgt agtcagtgat accggaattt ccggagaggg ccgcaggtat
     2701 attgcttctg tgcaattgat gcttatttac attggatgta gttatttctg catccaatgc
     2761 tatcggaaag agattaacca gcaaatggtt cctcaaatcg tattgatatt tacccggcgt
     2821 attgagcacc accatctgac actccgttcc cgcatcaaac gactggaccg aaaaacgatt
     2881 tacttttcac gtgctgtgga aattcacgaa aaaattattg ggtcattcat cgaaaaacat
     2941 atgttctgat gactggagtc atcctccaga aatatacgtc aactgaacaa tatcgatttt
     3001 ttatgggatt ctatacactt ttccatatcc ctctgaaaat cagtcatcag ctcaccaaca
     3061 cctcttctga aacttgtatt aaacttattg cttaaataga tgatataaca tctcttccat
     3121 tgcactgaat cggcgaaaac tgccgtttca ataatctcaa caatattatt tatcggctaa
     3181 ctttatcagt aaacaatgga atgtcacact gatgaaatga acattcagag taagatgata
     3241 aaattcatat aaacgccatt gctgaatatt atttatttca tacagataaa cgataaaata
     3301 caaaagggta tatttctcaa tgtaacagtc aattattcat ctccactggc gagcacggtc
     3361 agcacacaaa caaagttagt cctcctgctt aatcattgcg tatcagaaac taaaaaagaa
     3421 aaaattctgc agacaactta acaatcttca tcacatttcc tgaagacaga catatccaca
     3481 aacggtagtc agcgttgaat taacaagtct tacagagcta tacgtagctt ataaagccac
     3541 tcaatgttca tcatcacccg actacttatc tggaatacca ccagtataaa ctcatcaata
     3601 aaaacacctg ttgttaaaat aagaatactg tcagcagtaa attattaaaa caacatctgt
     3661 ccacactatc tgttctttta acgccaacgg cagcgaaaat agtgcatcac agccttcttt
     3721 tttctttcgc caccactcag acaaacacaa cactcacacc cacaccacaa aacagaatca
     3781 aaacataaaa ttaacaataa cagtcaaaag gaaaacacag aatattgagg tggactgtca
     3841 ccaggacagc gcatacgacg catgtgatca gcacatcccg ttcttcgctg gcatccccaa
     3901 aatgcagaac aaacaatcat aacattatga atataaaaga aaatatgatt tttcaccatg
     3961 aaaaaaacat caatgtatct ctgtataaaa ataatgataa catgacataa acatttaaaa
     4021 acataaataa acagcaacaa aaacatttga taatcactta gtgattaaat acaattaaga
     4081 cgattttgtt ataagagagg caccatggag tgagataatg aataacgaac agatgcttat
     4141 caaatgcaga gatccccgta aatgtcggtt ttatgccgaa tatctgaatc acaaataccc
     4201 taatgaattc cctcactttg cgcctgacaa cccacaaaat tgtatcagga atgttaattt
     4261 atttttaaag gaaaagtatt gtaatgtttc acctcccccc tttggtactt ttggtactac
     4321 tgaagaagaa gcactcaata acgaaattaa tgagcttgaa attattgccg ggcaggcaat
     4381 actgcctgaa gagagttttc tctggttgaa aactgacgaa cttgcgactt tctttacctg
     4441 gactactatt tatctgtcga aggaagacca tacaaaattc ccgaaaataa accataattc
     4501 atcaggaata aaaaacggaa ttgaagtcag gaaaatacga acagttaccg atgaaacgtt
     4561 aaacacttac aaaaaaagta accttccgga taatccatca tcccataacg aacgaataaa
     4621 agtaatcatt tcatattttg actcctgcca ttctttaatt aataaaaatg caaaaagcat
     4681 atacctcgag gaactcaggc aggcgtggct taaaaagaaa agcgcttgtc aaaagtttaa
     4741 gtggctcctt cctgacgatg atgaaatgtg tcagtggttc tggacaagac tgaaacaaaa
     4801 acaggatgaa aaacatccag tacataaccc agtgacccgg tggttcatac catcttcaac
     4861 gaaagaatat tatcttgcct ctcttgtcgc ctttgcttta tggaaagaag cgcctgatac
     4921 cattgaactg ttcagaacac gaataaatca tgcctggcat cagaaaaaac aaaaggacaa
     4981 atatcggagt gatggtaaaa aggccattaa tatacatata agaagcaatg tgaaagaaat
     5041 gcttgatgag ctcagaactg tttacggtct gagaacagga gagttttttg aaatgctgat
     5101 tacagagaaa tatcttgaac acaagcgaag gaagtaattc attacctgtt acattctaaa
     5161 taatctcaga cagatattat ctccgtgaat cccccgccac ctttccggtg cg
//
LOCUS       DAJTPG010000179         5205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTPG010000179 DAJTPG010000000
VERSION     DAJTPG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..529
                     /locus_tag="OO230_003865"
     CDS             <1..529
                     /locus_tag="OO230_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5693432.1"
                     /translation="GLGAITTGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPV
                     SKMTAGVYLRAFPRHIPAQNAVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQ
                     AVAMLETLREEQGSVLVHCALGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIV
                     LKEEHKAMLKLWENR"
     gene            complement(583..1188)
                     /gene="azoR"
                     /locus_tag="OO230_003866"
     CDS             complement(583..1188)
                     /gene="azoR"
                     /locus_tag="OO230_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5693433.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1389..>5205
                     /gene="hrpA"
                     /locus_tag="OO230_003867"
     CDS             1389..>5205
                     /gene="hrpA"
                     /locus_tag="OO230_003867"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5693434.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELR"
BASE COUNT         1281 a         1297 c         1458 g         1169 t
ORIGIN      
        1 tgggcttggc gcgataacaa cagggaaaga tagtcagggg aaactcccgc ccgccgttta
       61 ctggctgaca ttgccctggc gcatcgggat gtggctatct atgcgttggt tttgtcgtcg
      121 cctggagccg gtgagcaaaa tgactgctgg tgtttattta agggcgtttc cacgacatat
      181 tccggcacag aatgcggttc tggacgtcac ctttgaattc cctcgtggac gagcgacaaa
      241 agatcgactc tatttctgtg taccgatgtt ggatctggtg gttccggaag agggggagct
      301 ccgacaggcc gtggcgatgc tggaaacatt acgcgaagag caaggcagcg ttctggtcca
      361 ttgcgcattg ggattatcgc gcagtgcgct ggtagtggcg gcatggctgt tatgttacgg
      421 acactgtaaa acagttgatg aagcgattat ctttattcga gccagacgct cgcatattgt
      481 gcttaaggaa gagcacaaag cgatgttgaa attatgggaa aacaggtaag tggattgaga
      541 tgtggactga atatctacag tccacatcaa gaccgtgtcc ggttatgcag caacaatgct
      601 gtcgatggct gcttttgcgt cagactgtgc tttcgctgcc atttccggac cgtatgcgat
      661 cccttcggcg aagacaaatt tcacatcggt aatgccgata aagccgagga acgtggacag
      721 atacggcgtc accaggtccg ttggtccatc tttgtggatc ccaccgcggc tggtaataac
      781 gatggctttt ttacccgtta ccagaccttc cggaccgttc tcggtatagc ggaaagtaac
      841 gcctgcgcgt gcaaccaggt caaaatagtt tttcaactga gttgagatgt tgaagttata
      901 catcggtgcc gcaataacga taacgtcgtg ggctttcagc tcggcaatca gctcatcgga
      961 aagcgccaga gcttcctgct gacgcggagt cagcggcgca tcgctcggac gcagagcgcc
     1021 aaccagttcg ccatccagta ccggaatcgg atttgcagct aggtcgcgaa cggtgatttc
     1081 atcagcggag tgcttttcgc tccattgttc aacaaaataa tcggacaact gattagactg
     1141 agagtaccct gccaggatgc tggatttaag aactaatacc ttgctcatgg tgtttcctta
     1201 tagatgtttg aatggacgat gccccgttgc ttgctgacac tttattcaca atcctgccac
     1261 agagatcgcg caataaatcg aagtctatgt tcgaatttat tgaacaacgc atagaaagcc
     1321 gcgatgtggt actctatatc tatcatttaa aagaaaatta atcaggcaga ctactgccca
     1381 ctaacgttat gacagaacaa caaaaattga cctttactgc cttgcaacag cggctggatt
     1441 cgctgatgct gcgcgacaga ctgcgttttt ctcgccgtct gcattgtgtt aagaaggtta
     1501 aaaatcctga tgcacaacag gccattttcc aggagatggc gaaagagatt gaccaggcgg
     1561 cagggaaagt cctgctgcgt gaagcggcac gaccggaaat cacttatcct gacaatttac
     1621 cggttagtca gaaaaaacag gacatactcg aagcgattcg tgatcaccag gtggtgatcg
     1681 tcgccgggga aaccggctcc ggtaaaacga ctcagctacc gaaaatctgt atggagctgg
     1741 ggcgcggtat taaagggctg atcggtcata cccagccgcg tcgcctggcg gcacgaacgg
     1801 tggccaaccg tattgcggaa gagctgaaaa cggagccggg cggttgcatc ggttacaaag
     1861 tgcgtttcag cgatcacgta agtgataaca cgatggtcaa gctgatgacc gacgggatcc
     1921 tgctggcgga gatccagcaa gaccgcctgc tgatgcagta cgacaccatc attattgacg
     1981 aagcgcacga acgcagcctg aatatcgatt ttctgctcgg ctatttgaaa gagttgctgc
     2041 cgcggcgtcc tgacctaaaa atcattatca cttccgcgac tatcgacccg gaacgctttt
     2101 cgcgccactt taataatgcg ccgattattg aagtctccgg tcggacctat ccggtggaag
     2161 tgcgctatcg cccgattgtt gaagaagccg atgacaccga gcgcgatcag ttgcaggcga
     2221 tttttgacgc cgtagacgaa ctaagccagg aaagccccgg cgacattctg atctttatga
     2281 gcggcgagcg ggaaattcgc gataccgccg atgcgctgaa caagctgaac ttacgccata
     2341 ccgaaatctt gccgctttat gcgcggcttt cgaacagcga acagaaccgc gtgttccagt
     2401 cgcacagcgg acggcgcatt gtgctggcga ccaacgtcgc ggaaacctcg ctgaccgtac
     2461 cggggattaa atacgtcatc gaccccggta cagcgcgtat cagccgctac agctatcgca
     2521 ccaaagtgca gcgtttgccg attgaaccca tttcgcaggc gtctgccaat cagcgtaaag
     2581 gccgctgtgg tcgtgtgtcc gaagggatct gtattcgtct ctattccgaa gacgatttcc
     2641 tctcgcgccc ggagtttacc gatccggaga ttctgcgtac caacctggcc tcggttattt
     2701 tgcagatgac cgcgctgggg ctgggcgata tcgctgcgtt cccgtttgtc gaagcaccgg
     2761 ataaacgcaa tatccaggat ggcgtgcgtc tgctcgaaga actgggcgcg atcaccacgg
     2821 atgaacaggc cagcgcctat aaactgacgc cgctcggtcg ccagctctcg cagttgcctg
     2881 tcgacccacg tctggcgcgt atggtgctgg aagcgcaaaa acatggctgc gtgcgtgagg
     2941 cgatgattat cacgtccgcg ctctccatcc aggatccgcg cgaacgtccg atggacaaac
     3001 agcaggcatc ggacgaaaaa catcgtcgct tccacgacaa agaatccgac ttcctcgcgt
     3061 ttgtaaatct gtggaattat cttggcgagc agcaaaaggc gctttcttcc aacgccttcc
     3121 gtcgcctgtg tcgtaccgat tacctcaact atctgcgcgt gcgcgaatgg caggatatct
     3181 acacccagtt gcgccaggtg gtgaaagaac ttggcattcc ggtcaacagc gaaccggcgg
     3241 agtatcgtga aattcacatc gccttactga ccggtttgct ttcccatatc ggcatgaaag
     3301 atgccgataa acaggaatat accggtgcac gtaacgcgcg tttctccatc ttccccggtt
     3361 ctggtttatt caaaaagccg ccgaaatggg taatggtggc ggaactggta gaaaccagcc
     3421 gcctgtgggg gcgcattgct gcgcgtatcg acccggaatg ggtagaaccc gttgctcagc
     3481 atttgattaa acgcacctac agcgaaccgc actgggaacg ggcgcagggc gcggtgatgg
     3541 caacggaaaa agtcactgtt tatggtttgc cgattgttgc tgcgcgcaag gtcaactaca
     3601 gccagatcga ttcggcgtta tgtcgtgaac tctttattcg ccacgctctg gtggaaggtg
     3661 actggcagac gcgtcacgca ttcttccgtg aaaacctgaa actgcgggcc gaagtggaag
     3721 agctggaaca caaatcacgt cgccgcgata ttctggttga tgacgaaacg ctgtttgagt
     3781 tctacgacca gcgcatcagc cacgatgtaa tctccgctcg tcacttcgat agctggtgga
     3841 aaaaagtcag ccgcgaaacg cctgatttgc tcaactttga aaagagcatg ttgatcaaag
     3901 aaggggcgga aaaaatcagc aagctggatt acccgaactt ctggcatcag ggcaatctca
     3961 agctgcgttt gagctatcag tttgagccag gcgcggacgc ggacggtgtg accgtacata
     4021 ttccgctgcc gttacttaac caggttgagg aaagcgggtt tgaatggcag atccccggtc
     4081 tgcgccgcga attggtgatt gctctgatta aatcgttgcc gaaaccggta cgccgtaatt
     4141 ttgtgcctgc accaaactac gccgaagcgt ttttaggccg cgtcacaccg ctggagttac
     4201 cgttgctcga cagccttgag cgcgagttac ggcggatgac cggcgttacc gttgaccgcg
     4261 aagactggca ctgggatcag gtgcccgatc acctgaaaat caccttccgc gtggtggatg
     4321 acaaaaacaa gaagctaaaa gaagggcgct cgctacaaga tctgaaagat gcgctgaaag
     4381 gcaaagtgca ggaaacgctg tccgcggtgg cggatgacgg tatcgagcag agcggcttac
     4441 atatctggag ttttggtcag ctgccggaaa gttacgaaca gaagcgtggc aactataaag
     4501 tgaaggcgtg gccggcgctg gtggatgaac gcgacagcgt gacgatcaaa ctgtttgata
     4561 acccgctgga gcaaaaacag gcaatgtgga atggtcttcg ccgtctactg ctgctaaata
     4621 tttcctcgcc gatcaagtat ttacatgaga agttaccaaa caaagccaag ctggggctgt
     4681 actttaaccc gtatggcaaa gtgctggagc tgatcgacga ctgtatctcc tgcggtgtgg
     4741 atcaactgat cgacgccaat ggtggcccgg tctggacgga agaaggcttt gctgcgctgc
     4801 atgaaaaagt gcgcgccgaa ctgaatgaca cggtggtgga tattgcgaag caggtcgagc
     4861 aaatcctcac tgcggtgttc aatatcaaca aacgtctgaa agggcgggtg gatatgacca
     4921 tggcactggg gctttctgat attaaagcgc agatgggcgg gttggtatat cgcggttttg
     4981 tcactggtaa cggcttcaaa cggctgggcg acacgctgcg atatttgcag gcgattgaaa
     5041 aacgactgga aaaactggcg gttgatccac atcgcgatcg tgcacagatg ctgaaagtcg
     5101 aaaacgtcca gcaggcgtgg cagcaatgga tcaacaaact gccgcctgca cgtcgtgagg
     5161 atgaagacgt gaaagagatc cgttggatga tagaagagct gcgcg
//
LOCUS       DAJTPG010000180         5155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTPG010000180 DAJTPG010000000
VERSION     DAJTPG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO230_003868"
     CDS             complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO230_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5693435.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            895..1875
                     /gene="rluD"
                     /locus_tag="OO230_003869"
     CDS             895..1875
                     /gene="rluD"
                     /locus_tag="OO230_003869"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5693436.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1872..2603
                     /gene="pgeF"
                     /locus_tag="OO230_003870"
     CDS             1872..2603
                     /gene="pgeF"
                     /locus_tag="OO230_003870"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5693437.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2733..>5155
                     /gene="clpB"
                     /locus_tag="OO230_003871"
     CDS             2733..>5155
                     /gene="clpB"
                     /locus_tag="OO230_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5693438.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENG"
BASE COUNT         1263 a         1247 c         1407 g         1238 t
ORIGIN      
        1 cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg attttcgcta
       61 ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc agcggcagcg
      121 catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg cggttaacga
      181 cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca tatttcgcca
      241 gacgaccttt caggaatacc agacgtttgg tggcatcggt agtgtactga ctgttcggat
      301 agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga ggatcgcgat
      361 cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc atattggtca
      421 ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg cgaataaaac
      481 gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg tagatgagat
      541 ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac gcttccagtt
      601 gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc gcgtaaattt
      661 catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc aaaaacaggc
      721 ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga ctttcctcaa
      781 aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac aatagcacac
      841 tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt atatatggca
      901 caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg cttagatcag
      961 gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg gatcctcgac
     1021 cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt attgggtggc
     1081 gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc gcaggatatc
     1141 cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc gcgcgaccta
     1201 gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt gcttcattac
     1261 tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga taaagacacc
     1321 actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt cgaatctttg
     1381 caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat gaccgctggc
     1441 ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat ggcggtgcat
     1501 ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg tgtgcacacg
     1561 cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca tatggcccat
     1621 atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc gccaaaaggt
     1681 gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct tcatgcaacc
     1741 atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc gcctattcca
     1801 caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca taaggatgaa
     1861 gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa ggtgttgcgg
     1921 cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca ctcaacctcg
     1981 gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga ctttttgctg
     2041 cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa gatgtgctta
     2101 agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc aatacgcctg
     2161 gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc aatcgagcgg
     2221 gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc gtgctggaag
     2281 agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta gggccggcaa
     2341 ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg gcagtagacg
     2401 ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat atttatcagc
     2461 ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc gaccgttgta
     2521 catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc ggtcgtatgg
     2581 caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg cgtgattttc
     2641 ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca tttaatctcc
     2701 agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt actaataaat
     2761 tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac caattcatcg
     2821 aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt agtcctttat
     2881 taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag gcattaaatc
     2941 gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat ctggtgcgcg
     3001 ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc tcgtcagaac
     3061 tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg aaagcaacag
     3121 gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt gaaagcgtga
     3181 acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc gaccttaccg
     3241 aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa attcgccgta
     3301 ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt gaacccggcg
     3361 tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc gaagtgccgg
     3421 aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg gctggggcga
     3481 aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt gccaaacagg
     3541 aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg ggtaaagccg
     3601 atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt gaattgcact
     3661 gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat gctgcgctgg
     3721 aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc attgcgattc
     3781 tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact gacccggcaa
     3841 ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg ccggataaag
     3901 ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac tcaaaaccag
     3961 aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa caggcgttaa
     4021 tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa gaactgagcg
     4081 acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag gcatcgcttt
     4141 ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt gaacaggctc
     4201 gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc ccggaactgg
     4261 aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata
     4321 aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt ccggtttctc
     4381 gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg
     4441 tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg
     4501 ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca acgggtgtgg
     4561 ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac gaggcgatgg
     4621 tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg gttggtgcgc
     4681 ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg cgtcgtcgtc
     4741 cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc ttcaatattc
     4801 tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg gtcgacttcc
     4861 gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag gaacgcttcg
     4921 gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc cataacttcc
     4981 gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg ggtgaacagc
     5041 acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg gaagaacgtg
     5101 gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac ggtta
//
LOCUS       DAJTPG010000181         5152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTPG010000181 DAJTPG010000000
VERSION     DAJTPG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..220
                     /locus_tag="OO230_003872"
     CDS             <1..220
                     /locus_tag="OO230_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5693439.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="OO230_003873"
     CDS             complement(392..721)
                     /locus_tag="OO230_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5693440.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="OO230_003874"
     CDS             complement(726..911)
                     /locus_tag="OO230_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5693441.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="OO230_003875"
     CDS             complement(908..3547)
                     /locus_tag="OO230_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5693442.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="OO230_003876"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="OO230_003876"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5693443.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5152
                     /gene="hslJ"
                     /locus_tag="OO230_003877"
     CDS             4855..>5152
                     /gene="hslJ"
                     /locus_tag="OO230_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5693444.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLN"
BASE COUNT         1302 a         1320 c         1202 g         1328 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tg
//
LOCUS       DAJTPG010000182         5151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTPG010000182 DAJTPG010000000
VERSION     DAJTPG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..345
                     /locus_tag="OO230_003878"
     CDS             <1..345
                     /locus_tag="OO230_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5693445.1"
                     /translation="IAMLLNSLVGIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLL
                     GSFFVFASISGYQNVGAATTIAVLVASQLIGGLVLDIFRSHGVPLRALFGPICGAILL
                     VVGAWLVARRSF"
     gene            complement(346..579)
                     /locus_tag="OO230_003879"
     CDS             complement(346..579)
                     /locus_tag="OO230_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5693446.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(665..838)
                     /gene="ortT"
                     /locus_tag="OO230_003880"
     CDS             complement(665..838)
                     /gene="ortT"
                     /locus_tag="OO230_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5693447.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1033..1128
                     /gene="yncL"
                     /locus_tag="OO230_003881"
     CDS             1033..1128
                     /gene="yncL"
                     /locus_tag="OO230_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5693448.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1530..2339)
                     /locus_tag="OO230_003882"
     CDS             complement(1530..2339)
                     /locus_tag="OO230_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5693449.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(2549..3973)
                     /gene="patD"
                     /locus_tag="OO230_003883"
     CDS             complement(2549..3973)
                     /gene="patD"
                     /locus_tag="OO230_003883"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5693450.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3995..4789)
                     /locus_tag="OO230_003884"
     CDS             complement(3995..4789)
                     /locus_tag="OO230_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5693451.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4779..>5151)
                     /locus_tag="OO230_003885"
     CDS             complement(4779..>5151)
                     /locus_tag="OO230_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5693452.1"
                     /translation="RSRQAAYSMILPVQAALERLPPSLLQASADLGARPRQTFRYVVL
                     PLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLV
                     PIILIALYLAFVKRLGAFDAL"
BASE COUNT         1376 a         1320 c         1112 g         1343 t
ORIGIN      
        1 atcgccatgt tgctgaactc actggtgggg attgtgctct ttgtttctat tttatggttt
       61 aaacaaggca tggcggggtt tggcgaactg gtgtccagcg tgcgctggtg gacacttata
      121 cctggcttgc tgggttcgtt ttttgtcttc gccagtatca gcggatatca gaatgtggga
      181 gcggcgacaa ctattgcggt actggtcgcc agccagctga ttggcggtct ggtgctggat
      241 atcttccgca gtcatggcgt accactacgg gcgttgttcg gcccaatatg tggcgcaatt
      301 ttacttgtgg ttggcgcctg gttggtggcc agacgctcat tttaatcaga ggatggtgcc
      361 gcctttggtt aactgttcgt ggcgcgcttc catatcttct ttatgcttgc gaccgtgatg
      421 agcaatagcg gtgcgcaaac gctgctgctg ggtgtagcga tcttcccgac ttaactccgc
      481 gtcatcgctc agggcgatta ataattcgtt catatgggca atgacgctct cttcaatcgc
      541 ggcatccacg cgcgcgatga cttcatccag atgcgacata ctttctcctt agccacttgt
      601 gaaatttatc gattaatcca aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta
      661 agatttaaaa cagtgaaaac aacagcgcca ccgggaaact catcggccat gtggccccca
      721 ccagaaaggc gcttaagaaa cggatgcgtt tcttatcgtg agaaagaaac caggtgataa
      781 gaaatgcgat tgctgccata accgcataaa aaacaagcat atgttgatag agaaacatgt
      841 acgggcgtcc agccatatga ataaaacgcg cgcaaatatg cgccattttt tgacgtgcat
      901 caagtttttt caccagtttg agaaaaaatc atcaaacagc aacttgccct tatccatctt
      961 cagaatgagt attattgcgg attttcttaa cccgtactat acacataggg gcaatgataa
     1021 aaggtggcaa aaatgaatgt ttccagtaga actgtagtac tgataaatgt ctttgctgct
     1081 gttggtttgt ttactcttat ctctatgaga tttggctggt ttatttgatg tagtgatttt
     1141 gcacgcagca cgtactgtca gagaaatcaa ctctctgaca gtacgcaaga tcttctcata
     1201 ctctccaagt ttgcaacatt atccttaata tttacttctt tttgctggca tctacattga
     1261 aatgagtaga aaacatcccc ctactgtcgt ctatacaaac agcttactga ttcaaacgga
     1321 atcttgtttt cacatttttc tatcacgaat actttatacg cgcgggagtc tataaatgct
     1381 ttatactttc aaggaataac gtaaatataa aaagatggcg taaagtatat gcatggcagg
     1441 atgtttgcaa cctccttgat gacgctgccc cattgaaatt gcaaaccccg ccctggttat
     1501 cttgcccatt tatcatgcaa aacagataat tataggcctc tgaatacctc agaaagattg
     1561 aattttttat agtccctctc ttgaatattt ccatttgata tcaatcgatt catctcttca
     1621 acaaaataat ttatatcatc acacttatta ttatccggaa gatctgagta gttataattc
     1681 caccatttta ttctcaacag ttcatcaata acatttgaat caaacctaaa tctgattatt
     1741 tttgcaggaa ctcctgcaac tatggcatat gggggcacat ctttggtaac aacggaattg
     1801 gcagcaataa cagcaccatc acctattgcg atgccacctt tcaatacaac atcatttcct
     1861 atccagacat catttcctat aatcggattt tcaggtttgg taatgaatgg tttcaatgtc
     1921 cagctacgtt caaattcact catagcaaac ttatcaaatt caccgttata agtcatcatg
     1981 tgagtagtaa aacgattcaa aggatgattt ccacccatta tttttacatt tgatgcaata
     2041 ctacaaaatc gacctatacg tatattccct gaaaaatggc aggtcggaaa tgaaaaagcg
     2101 ccgacactgg agaaattttt cacaggcatt gttgaatatt cttcagcaat aacattatct
     2161 tcaattgtta tcttgtcacc ttttgagaaa agaaataaat tcctttcttt atacgacttc
     2221 ttccccttat gatttaaaaa gattttatta tccatacaaa actcaaagtg ttttttcgtc
     2281 catctgaagg taagggtttt atttttatct tccgtgaatt tgtacaattg aactttcatt
     2341 gttaatactt ctacttataa agtattagtt taataacgat actctaaagc cttatgctaa
     2401 tcaactgtga caggatgaaa aatcgccatc aaacatttag cctaattttt acgccagtta
     2461 ttaatgtaat cattctaata cttaaccgcc attaaactca gaacctaaaa cgatagtggc
     2521 cgtcctataa cagccactaa catctaactt aatgtttaac catgacgtgg cggacgacgg
     2581 tgtaatcctc cagcccataa agtgacatat ccttgccgta accagaaagt ttctgcccac
     2641 cgtgcggcat ttcacttacc agcatgaaat gggtattgac ccaggtacaa ccatattgca
     2701 gccgtgcgct gacgcgatgc gccctgccca catctttcgt ccataccgaa gatgcaagtc
     2761 cgtactggct gtcattcgcc cagttcacca cctgttcttc gttgtcgaag ggcgtaacac
     2821 tcactactgg accaaatacc tctttttgca cgatggcatc gtcctgtaat gcgccagcca
     2881 gcagcgtcgg cgcatagtaa tagccattac ccttgcgctt ttcaccgcca gtgatcactt
     2941 tgatgtgccc tgtcgctttc gcctcttcta ctgccttgct gacgcgttcg agatgcgcca
     3001 gcgagcttaa aggtccaagc tccgtagact cgtcatccgg cgcaccagat tttaacgttg
     3061 ccaccgcagc acccagtttt tccaccagcg tatcgtaaat gcctttttgc gcgtagatcc
     3121 gacaagccgc agtacaatcc tgtccagcat tgtaatagcc aaatgtacgt acaccttcga
     3181 ccactgcttc aatatccgca tcatcaaaaa caatcactgg cgctttgcca ccaagttcca
     3241 tatgagtacg cttaatggac gacgcggtat ggctgatgat gtgctcgccg gtggcgatag
     3301 agcccgtcag cgacaccatc cgcactttgg gatgaccggt cagcggatca cccaccgttt
     3361 tgcctctgcc aaacagtatg ttaatcacgc ctgccgggaa gatatctttc gccagctctg
     3421 ccaacttcaa cgcggtcagc ggggtaattt ctgatggttt aagcactacg cagttccctg
     3481 ccgccagcgc cggagcaagt ttccacgcgg ccatcatcag cggataattc cacggtgcga
     3541 tagaagccac gacccccaac ggatcgcgac ggatcatcga agtatgacct tcaagatatt
     3601 cacctgccgc cagaccattc agacagcgcg ccgcacccgc gaaaaagcga aaaacatcga
     3661 caatcgccgg gatttcatca ttgaacgcac tatgcagcgg tttgccacaa ttacgggatt
     3721 ccagttcggc aaaaacctga ccattttctt cgataacatc cgccagtttc agcaggcatt
     3781 ccgcacgcgc tttcggtgtg gtttgccccc attcagcaaa tgctgcatct gccgcacgca
     3841 cagcagcatc gacctgctct gcggatacct cggcaatctc cagtaaaacg tcccccgttg
     3901 ccggattata gacaggctgt ttttcccctt cgccgctaac ccgttctccg ttaatcagta
     3961 acttatgttg catagcttat tcctgtatca gtggttattg accattgtcg ccttcgcggg
     4021 ttagccacca ggcccccagg atcggcaagg ttgttaccaa cataaccagc agtgccacca
     4081 cgttggttac cggtacatca cgcggtcgcc caagctgatt gagcaaccac aacggtaacg
     4141 ttcgttcatg acctgccgta aaggtcgtaa cgatgatttc atcgaacgac aaggcaaacg
     4201 ccagcattcc tcctgccagt aacgccgaac tgagattcgg caacactacg tagcggaagg
     4261 tttgccagcc attggcccca agatccattg acgcctcaac cagactccac gaggtgcggc
     4321 gaaaacgggc gatgacattg ttaaacacca ccactacgca aaaagtcgca tgaccgacca
     4381 cgatggtgaa aaatcccggc tccagattga tggttttaaa ggcggttaat agcgccagac
     4441 cagtgacaat gcccggcagc gcaatgggca gcagcaataa cagcgaaatg gcgtttttgc
     4501 cgaaaaagtc tcgtctccac agcgcggcag ctgccagcgt ccctaacacc agcgcaatta
     4561 atgtcgccag cgccgccact ttaagtgaca gcgtcacggc atcaagaata tcactacgct
     4621 gtgctgccac gctaaaccag cgcagcgtca ggccctgcgg tggaaaacta aacgccgcat
     4681 cttcagtgtt aaacgcatag gcggcgatta tcaggatggg aaaatgtagg aaaacaacgc
     4741 cgccccaggc cgccagtttg aggaaaaacg gtgcgcgttc agagtgcatc gaacgctccc
     4801 agacgtttca cgaacgccag gtacagtgcg atgagaataa tcggcaccag ggtgaatgcc
     4861 gccgccatcg gcatattgcc aatcgccccc tgctgggaat aaaccatatt gccgataaaa
     4921 tatcctggag gaccaaccag ctgcgggacg ataaaatcgc ccagtgtgag tgagaaggta
     4981 aagatagagc cagcggcaat acccgggatt gccagcggca gcaccacata gcgaaaggtt
     5041 tgtcgtggac gtgcgccgag atcagccgac gcctgcaaca atgacggcgg caaacgctca
     5101 agcgccgcct gaacgggcag gatcatcgag taggcagcct gacggctgcg g
//
LOCUS       DAJTPG010000183         5035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTPG010000183 DAJTPG010000000
VERSION     DAJTPG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(684..1100)
                     /locus_tag="OO230_003886"
     CDS             complement(684..1100)
                     /locus_tag="OO230_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693453.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1127..1267
                     /locus_tag="OO230_003887"
                     /pseudo
     CDS             1127..1267
                     /locus_tag="OO230_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1267..2292
                     /locus_tag="OO230_003888"
     CDS             1267..2292
                     /locus_tag="OO230_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5693454.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2345..2434)
                     /locus_tag="OO230_003889"
     tRNA            complement(2345..2434)
                     /locus_tag="OO230_003889"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2398..2400),aa:Ser,seq:cga)
     gene            2528..3325
                     /gene="mtfA"
                     /locus_tag="OO230_003890"
     CDS             2528..3325
                     /gene="mtfA"
                     /locus_tag="OO230_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5693455.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3426..3501
                     /locus_tag="OO230_003891"
     tRNA            3426..3501
                     /locus_tag="OO230_003891"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3459..3461,aa:Asn,seq:gtt)
     gene            3663..4925
                     /locus_tag="OO230_003892"
                     /pseudo
     CDS             3663..4925
                     /locus_tag="OO230_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1392 a         1184 c         1128 g         1331 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta attgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1021 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1081 tcttttgtta attcagtcat tttcattacc gccctggtaa tgctgccacc agcgaaaatg
     1141 gttaatggaa aacgggcttc cgttctatac caatcgtagt ggcaaaccga ttgtcagccg
     1201 ggatctattt acctgcaata aaactttacc accacgcgag gtagagccga attttggtgc
     1261 gatctgatgg gaagacgaag gaaaaatcct gaacacgaaa aattacctcc aaatgtatac
     1321 ccaaataaat atagttatgt atggaaacca acatccagag aatctgtaac actaaccgcc
     1381 atcaaggatg gtttagctgc tttatggaaa aagtatgagg aaactgtaaa taatcgcgat
     1441 cgtgcaatga cattcggtcg cttgtgggaa aaattcctcg ccagcgccta ttacagtgac
     1501 ctcagtccaa gaacacaaaa agattatctg caacatcaaa aaaagttgct tgccgtattc
     1561 ggtaaggtgc cggcagattc cataaaacca gaacacatcc gtcgatacat ggacaagaga
     1621 ggggaacaga gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg cgtttacagt
     1681 tgggggtatg agcgagggta cgtgaaggct aacccatgtg caggtgtaag taaattcaag
     1741 gccaaaaacc gcgaacgata tgtaaccgac aaagaatacc aggcagtatt aagcgttgca
     1801 cctcttcctg tttttatcgc aatggaaatt gcctatctgt gtgcagcgag ggtttccgat
     1861 gtgttatcgc tgaaatggga gcagattgga aacgacggga tctttatcca gcaagggaaa
     1921 acaggaaaaa aacagataaa agcatggagt ccacgattac aggcggcgat cgaaaaagca
     1981 aaacagttac caacatccgc ctatgtaatc agcaatcaat acggcaaccg atatatgtac
     2041 aaaggcttta acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt
     2101 ttaaccgact tcacctttca tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc
     2161 agtcgggata agcaactttt ctctggtcac aaaaccgagg ggcaagtgct aatctatgac
     2221 aggaaggtta aagtttcacc gacacttgat gtcccgttac ctgaaaatat tccaagaaaa
     2281 tattccaagt aattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga
     2341 attttggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg
     2401 agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca tgatgccagg
     2461 aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag
     2521 caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc
     2581 ctggcaggaa gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa
     2641 attggtcgct cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt
     2701 tgagctgaat tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga
     2761 gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt
     2821 ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc
     2881 aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt
     2941 tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa
     3001 cggcgatcgc gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca
     3061 cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc
     3121 ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga
     3181 atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt
     3241 ctgtcaattt tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc
     3301 gttttcagcg acgaatgttc attaattaac aactttgaag attaattaac caattgaaat
     3361 ggcttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc
     3421 acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     3481 cgagtccagt cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga
     3541 tgttgattca acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc
     3601 atcgggaaaa attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc
     3661 atatatccct taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag
     3721 tctccgattc tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc
     3781 tcaaataccg tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct
     3841 ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca
     3901 acccggtaca gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa
     3961 acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc
     4021 tgcttgccag cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac
     4081 ttaaaccccg tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg
     4141 ttgcgtcccg cacacggcag cacctgagta acataatgcg ccatgcggtc catcaggagt
     4201 taatcgatac gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc
     4261 actatcctgc cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc
     4321 atcagggccg tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct
     4381 ccagtgaact gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga
     4441 tacccgcgac gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc
     4501 gaatgccgca tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg
     4561 atatcaccgg taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt
     4621 gtgaaaacac ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct
     4681 gcggtcacgg cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa
     4741 aggacgcagt agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata
     4801 ttcataaggc agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc
     4861 tggaagcatg ccgagaatct tatgcaccgc cttatacaat tggtaaaaat aagtttatcc
     4921 catagtatac taacgaatcc cacactctgg caaattacca ccacctctac aggaggtggt
     4981 tctatataga cttttatcaa cttcatgacg aaaaacacct atttgaagtg gtcaa
//
LOCUS       DAJTPG010000184         5018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTPG010000184 DAJTPG010000000
VERSION     DAJTPG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(241..486)
                     /locus_tag="OO230_003893"
     CDS             complement(241..486)
                     /locus_tag="OO230_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693456.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1263..2105)
                     /locus_tag="OO230_003894"
     CDS             complement(1263..2105)
                     /locus_tag="OO230_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5693457.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2190..2387)
                     /locus_tag="OO230_003895"
     CDS             complement(2190..2387)
                     /locus_tag="OO230_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693458.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2407..2895)
                     /locus_tag="OO230_003896"
     CDS             complement(2407..2895)
                     /locus_tag="OO230_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693459.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2892..3269)
                     /locus_tag="OO230_003897"
     CDS             complement(2892..3269)
                     /locus_tag="OO230_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5693460.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3316..3693)
                     /gene="cbtA"
                     /locus_tag="OO230_003898"
     CDS             complement(3316..3693)
                     /gene="cbtA"
                     /locus_tag="OO230_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR5693461.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3773..3994)
                     /locus_tag="OO230_003899"
     CDS             complement(3773..3994)
                     /locus_tag="OO230_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5693462.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4081..4557)
                     /locus_tag="OO230_003900"
     CDS             complement(4081..4557)
                     /locus_tag="OO230_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5693463.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4573..>5018)
                     /locus_tag="OO230_003901"
     CDS             complement(4573..>5018)
                     /locus_tag="OO230_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5693464.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1164 a         1220 c         1334 g         1300 t
ORIGIN      
        1 aagtatgtca ggcttttttc cgtaatgctc taacttccac gcattggttt ctgacatcaa
       61 ataaggctat gactttctct cccttttaat ctcattctga tctaaaacca tatcttaaaa
      121 aaatgcatgg gtattttaat tattttgatg ttgatctaac gcactcaagg accattttat
      181 taccgattat ccattctatt taactgttgc gatataaatg tttgttttag gtgctttaat
      241 tcagtacaag tcatcaaacc gtaccagacc ctgactgtca gtgacaccga caggtggggg
      301 cggcggtgtg gggtcaaact gagtgtgtct gcctctggcc cacagagcca gctctgccgc
      361 cattgcccgt ggatgatact ccagctgcac gttgagaccg gaaactgggt tgttgttctc
      421 atccacgaac tgcagcgcca gccagtgcat ccgctgctta ccgcaggcga cttctttgta
      481 cttcattttg tgactcaggc aggtgactgt cgtgttgcct gttgcgtcca ttttgcggtt
      541 aaaccctttt aattcaattt gttaaattgc tttccacctt ttgcggggcg cgacttaacc
      601 aaaatattca ctgttatcat aatatgcaat gggctggagg ttgcaggtca tttagggatt
      661 ttctgtaagc cccgttttgc agaacgttct atctccgatt gagatcctgt ctctcggtat
      721 taccggtagt ctggccataa tcccctatgg atcatattga tatagtaggc ataacaccca
      781 aaagttgact aaagctaaat caatcagagt gaaaaatgtt cggtttgatg ttggggggga
      841 ctgaggaggg gccaacgcag gtggtatgcg ttttacttca gaccggacaa agaccggatg
      901 tatccgtgca gtaatgaatc cgttagccag gtgctcatgt ggaaatagtg tctattgggg
      961 atttgaagtt tgacagaaaa ggccacagtg accagaaggg ggctacttag aagaaatgtt
     1021 caggctgggt ggaagaggta aagcagtgac atcagcattc aaagtgaccg gatatctacc
     1081 agaaccccag aaagcaaaaa accagcccgt aggctggttc ttctaaatag tggtgccgga
     1141 ctcggaatcg aaaatttctt ttatcatatt gatattaatg caatttgttt ggtttgatta
     1201 aaaatgtacc ctcaattata cccgcagtcc cttttgatat cgaaagggaa tcactctcgg
     1261 gatcaggctg agagcacaga aggaaagtac ctggctatta tatcgttaat tctgtcaatc
     1321 agttccagcc ttttgaaagt gatgtgtgca gatcccttct taaagtattt aatgatgaac
     1381 atctcgtctt catagcgctc tttaccctga acggactgga tgtgaccatc cagacgaata
     1441 gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acagatgtag cattctttcc
     1501 aggtcgacga gtcggtcagt ctgttgcccg gtgataagat gaaaccccca ccggtcccac
     1561 ctcaccaggt tgttgacgat aattttactg ccaaatttac agggtgagtt ggttttgtaa
     1621 tcccagctca gccctttgaa gacgttgatc actccccgtt caaacacttc atctttgttc
     1681 tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatca
     1741 tattccagtg acctgtacca tgtgtcccgg gtctgcgcat ccatcagact cagcatcccg
     1801 gaacgttgca tcagatcacg ccagagttcg cgatcaagat tgcgtgtgat ggctttcatt
     1861 gcggtttccg gtttctccat cagccagcaa ccgcagcgaa aatcctgttt catggcccag
     1921 tccagagccg ttttacctct aatactttct gtcattgctg atacttcagc cagttgtcgg
     1981 ataagtgtgt cgatttgcag caatgtactc tttcgtacag ccagaatgtg gcggatactg
     2041 gttgagcaga taacgtccgt atgcccgttg accacttccg gacgagaggc tgtgatttca
     2101 gacataatcg atattccttt ttgcagataa acgaaaacgc cagccgtatt caggctggcg
     2161 tcagtggaat gaagtctggt gagtaagact caccggttct gttgcagaaa ctgcagctga
     2221 cgcagggtac ggatatcttc cctggcctgt tcaatacagg gcagcagtgc tgccgaatcc
     2281 gttttatcct catcgttgtc gaagataatc cctgattcgc agtcaatatt gtcctgcagc
     2341 cacgcaatca gaatatccag cgccgtttct gtggttaata atttcatcca gtgtcttttg
     2401 tcctgattac cagtcgaaag tggggaaccc cggcagacat tctgcgccgg cgtccagatg
     2461 aatgagactg acgccataac gccgggtgag agtgacgacc agacgacgga acgttctgga
     2521 caggcccagg cgtttaagcc gcagaaccgg gtacgaccat gcatcggtac gtaacagata
     2581 gccgctgcct gtaaaatgaa tccattctgt ttcgccgtcg tcattagtct ggtgtgacag
     2641 cgagtacagc caggcgttgt ccgtttccgt gatatgtgcg gtgctgcaac gtatgcaggt
     2701 aagggttgtt aacggtggtg gtgtactggt tgcgtgttgg tcggatttat cgactacgcg
     2761 gagtgaataa ccgttttcag agaccttgtt aatcagttca ctgagattaa caccatcgac
     2821 gtcaacgaca atgcgcccca tgttcagtgc ctgcacgtta acgctgtcgg cttccagcgt
     2881 cagggccagt ttcattgttt cgcttccgga tgcttccccc gggtaatgtt atttaccgtt
     2941 ctgtagttat cgcgggtcat caggccggtt gcccggcgtg cccggagaat atcaatgctg
     3001 ttgattaact gcgacggggc tcctgcgctg aatcccggct ggtcggtacg taccagcgcg
     3061 tatttttcaa cgagaaagtt caccgcgtca cacagtgaaa ttcctgcctc aatatgctgc
     3121 tcaatcacac gttcatcagc gaacggtgtg tcattcagcg tgaggccata gtgctggtcc
     3181 agcagtcggg tgagcagtgt ctgccagatg gtgatgggag acgggcagcc cgatgcctcc
     3241 cgtacgtgag tgtcgggtaa tgtattcatc ttaagttctc tgtaacaggt ggttataaag
     3301 gggagatatc cggtgttata cggtggttgc gggcgctgcc ggtgtcggat aaacagccag
     3361 ataaacgtaa ccacaggagc cgagggtgtc ggcttcgcag gtcagccctt tcgcatacag
     3421 tgtgacggta tgctgatggc ggggattcag ttcaccgcca gtgagcatga gttccagttg
     3481 tttcatcagc agcggaaagg cctggtccag gtggtacgca tctacgtcgc tgaacctgcc
     3541 tctgataccg gcgcggtctg caaggtaatg caggcggtta ccctcctgta ccagtcgggc
     3601 cccgaaacag ggcgtcacgg tgcagggcag cccccaccag gggcggtcgt gattgtcgtc
     3661 gggaggcgtt gtcccgggga gtgtgtctga cacgataaag tcctcacaga aaatcggtta
     3721 agaatggtcc ggtagtggcg ataattctgc tcatcagaat tcccttccag actcagttca
     3781 gggtgacgct catcagccgg acatacggac caaaactgtc cttacggcgt tctgcaaaca
     3841 cagccagtac accgggaata tcctgcactt cacggccggt atatgtatcc gtgctaccgt
     3901 gccagcggta tttaccggta cagaacggaa aaagacggga tgcaggatgc tgacggtgaa
     3961 tacgcatggc ttcaccacgg gtgatgattt tcatgagaat agtcctcctg aatgtgaaag
     4021 taagaaacag ctgagaaaga gaaactgaag tgatatgaag gtgattgtga cggtgacggg
     4081 ttaaagcaga ccgtgttccg caaaggagaa aacctggctg ccaccgacta tcagatggtc
     4141 cggtacccgg atatcaacca gggccagtgc ctgtaccaga cgctcagtga taaggcggtc
     4201 tgccttgctg ggggggactt caccggacgg gtgattgtgc gccagcacca cggcagcggc
     4261 attgtggtac agggcgcgtt taatcacttc ccggggatgg acttccgtac ggttgatggt
     4321 gccggtgaag agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg
     4381 gaactcttca cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ccgcatgcgt
     4441 ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggttttca gggcccgcag
     4501 aatgaggctg cgctctctgg ttgtcatttc tccgggcaga aaggaaagct gctgcattgt
     4561 ccctgccacc attcagtcga tgatgcgcat aatggcgctg cattccggat gctgcagggc
     4621 gtaatcccgc agccggtaat aatggaccgt catggcataa cactccgtac gacaggcatg
     4681 atgactgtac gtcatcagac aggcagcaat gccggcagct tccggactca tttcagcgcg
     4741 gttaccgttc atggcattga acagtaccca tgtttcgtca tcatcgtcat ccggttcggg
     4801 tgccataaat gccccgccgt tgttcagggt gtacagattc cagataccac cgcagtagtc
     4861 ttcgcacaga cggtccatcc agccgaagac acggggctcc agggtcaccc actgtggaat
     4921 gaggccaaag tgctgcggcc agaagctgat gcgctgttca tcagggacga gggtggcaac
     4981 cagttgttgc aggtcagtct ccggtgcaca ggccgaag
//
LOCUS       DAJTPG010000185         5011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTPG010000185 DAJTPG010000000
VERSION     DAJTPG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..586
                     /locus_tag="OO230_003902"
     CDS             <1..586
                     /locus_tag="OO230_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5693465.1"
                     /translation="TNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHM
                     YKIATLGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCS
                     SEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNK
                     SKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            920..3199
                     /gene="ydeP"
                     /locus_tag="OO230_003903"
     CDS             920..3199
                     /gene="ydeP"
                     /locus_tag="OO230_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5693466.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3447..3650
                     /gene="safA"
                     /locus_tag="OO230_003904"
     CDS             3447..3650
                     /gene="safA"
                     /locus_tag="OO230_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5693467.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3718..4479
                     /gene="ydeO"
                     /locus_tag="OO230_003905"
     CDS             3718..4479
                     /gene="ydeO"
                     /locus_tag="OO230_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5693468.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4881..>5011
                     /locus_tag="OO230_003906"
     CDS             4881..>5011
                     /locus_tag="OO230_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5693469.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVA"
BASE COUNT         1437 a         1060 c         1119 g         1395 t
ORIGIN      
        1 gacaaatacc aacgtattgg atattggtga taagacaccg atgccattac cgttaaaact
       61 ctatattacc ccagttgggg cggccggtgg agttgtcata aaagcggggg aagtcattgc
      121 ccgtattcat atgtataaaa ttgcaacatt aggaagcgga aatccgcgta attttacgtg
      181 gaatattatt tcaaataaca gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg
      241 taatgtcacc gtggatctcc cggactttcc tggaagcgca gaaatacccc ttggcgtata
      301 ttgctcaagc gaacaaaagc tctcatttta tctttcgggt gccaccactg acagtagccg
      361 acaagtgttt gctaacacag cacctgatgc aacaaaagca tcgggagtgg gtgtcactct
      421 aatgcgtaat ggaaaaatac tggctacggg agaaaatgtc tccctgggga cagtaaataa
      481 aagcaaagta cctctgggtt taagcgcaac ctatggacaa acaggaaaca aggtttcagc
      541 cggaaccgtc cagtcagtca ttggtgtcac ttttatctat gaataattgt gattttgacc
      601 ttagtctatg cgctccattc acgaaaattg cgcggataat tattttgtga aggctattat
      661 cctacacctg taagaaaatc cgcgcacaat tgaagccaga gtcttatttt ttatattgga
      721 aaatatgtta atttaaacac tatgagagat gatgcttaac ttatcgtatt taatctattg
      781 tttaacgata atagattaaa aatagaaaga gagtagtccc ttactcttat atatccatgt
      841 tggcgataat ccctttgtat gtactgtgca tcatcgctat tacaaatcct aataattcat
      901 ttccacacag gataagtaga tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg
      961 ttggggtgct gttaaatccg tagcgaatgc agtacgtaag cagatggata tacgccatga
     1021 tgttattgcc atgtttgaca tgaataagcc agagggcttt gactgtccgg gttgtgcatg
     1081 gccagatcct aagaacagtg cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc
     1141 ttgggaagtc acggataagc aggtaaatgc ctctttcttt gctgagaata cggttcaatc
     1201 attacttacc tggggagatc acgagcttga ggctgcgggg cgtctcactc agcctttgaa
     1261 atatgatgcc gtcagcgact gttacaagcc attaagctgg caacaagctt tcgacgaaat
     1321 tggcgcacgc cttcaaagct atagtgatcc caatcaggtt gaattctata cttcgggccg
     1381 cacttccaat gaagctgcct ttctttatca gctttttgcc cgtgaatacg ggagcaataa
     1441 ctttcccgac tgctccaaca tgtgccatga accgacaagc gtgggtttgg cagcgagtat
     1501 cggtgtaggt aaagggaccg tgttgctgga agactttgag aagtgcgatt tagtcatttg
     1561 cattgggcat aaccctggta caaaccaccc tcgcatgctg acttcgttgc gcgctttagt
     1621 gaaacgggga gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc tggagcgatt
     1681 taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt tggccagtgc
     1741 ctactacaac gtgcgcattg gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt
     1801 aattgagcgc gatgatgctg caagtgccgc aggtcggccc tcattgcttg atgacgaatt
     1861 tattcaaacg cataccgtcg gctttgacga gctacgccgt gacgttctca attccgagtg
     1921 gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac tggctgacgc
     1981 atatgccgct gccgaacgca ccattatctg ttacggaatg gggatcactc agcacgaaca
     2041 tggtacccag aacgtgcagc aactggtcaa tttgctgttg atgaaaggta acattggcaa
     2101 gcctggtgcg ggtatctgcc cactacgtgg tcactctaat gtacagggcg accgaaccgt
     2161 cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc ctgggtgagc gctatggctt
     2221 ccccccacct catgcacctg gacatgctgc aattgccagc atgcaagcaa tatgtacggg
     2281 gcaggctcga gcattgatct gcatgggggg caactttgca ctggcaatgc cagatcggga
     2341 agcgagcgct gtaccgttaa cgcaattaga tttggcggta cacgtagcca ctaagcttaa
     2401 ccgctctcat ctgctgaccg cacggcatag ctatattctg ccggttctgg ggcgtagcga
     2461 gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat
     2521 tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt
     2581 ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg gtagcctggg agtatctggt
     2641 ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt tcgccgacta
     2701 taaccagcgc atccgtcatc ccggtggttt tcacctgata aatgcagctg ctgaaaggcg
     2761 ctggatgacg ccgtcaggta aggctaattt cattaccagc aaagggctgt tagaagatcc
     2821 ctcttcagcg tttaacagta agctggtcat ggcgacagta cgcagccacg atcagtacaa
     2881 cacgacgatt tatggtatgg atgatcgcta tcgaggggta ttcggtcaac gagatgtggt
     2941 ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct
     3001 tattgcgctt acgccagacg gtaagcgcag ctcacgccgc atggatagat taaaagtggt
     3061 catttaccct atggctgacc gctcactggt gacctatttt ccagaatcga atcacatgct
     3121 aacacttgat aaccacgatc cattaagtgg tattcctggc tataaaagta ttccggttga
     3181 attagaacca tcaaattaat gcctcttctc atttcttctg ctgtcatccg cacagcagaa
     3241 gaattcctca ttgactatta ttgcgcaatt tgctcacatg gattaaatta aactacatac
     3301 tataagatat aaacttctgc ctacagctgt aagaaactcc gctcagtact gaagcaccag
     3361 tcctatttct tcttttctcc agcctgttat attaagcata ctgattaacg acttttaacg
     3421 ttatccgcta aataaacata tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc
     3481 aaagagataa ctataaagaa atcatgtctg taattgtggt tgtcttatta ctgacactta
     3541 cgttgatagc cattttttcg gcaattgatc agctgagtat ttcagaaatg ggtcgcattg
     3601 caagagatct tacacatttc attatcaata gtttgcaaga ctggaaataa acagcaaatt
     3661 ataaatgtga aatgtttaaa aggtatcgat aaaactttat tgttttaagg agataaaatg
     3721 tcgctcgttt gttctgttat atttattcat catgctttca acgctaacat tttagataaa
     3781 gattacgcct tctccgacgg cgagatcctg atggtagata acgctgttcg tacacatttt
     3841 gaaccttatg agcggcattt taaagaaatc ggatttaatg aaaataccat taaaaaatat
     3901 ctacaatgca ctaacatcca gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct
     3961 tcaaatgtac cgactggatt gcttaatgaa atgattgctt atctcaactc ggaagaacgc
     4021 aatcatcata atttttcaga acttttgctt ttttcttgcc tgtctatttt tgccgcatgc
     4081 aaaggtttca ttacactatt aactaacggt gtgctatccg tttccgggaa agtgagaaat
     4141 attgtcaaca tgaagctggc gcacccatgg aagctgaaag atatttgtga ctgcctgtac
     4201 atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc aaacgacatt ctcacagatt
     4261 ctattagatg ccagaatgca gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat
     4321 aaaattgccg aacaatgtgg ttatgccagt acatcttatt ttatttatgc gttccgcaaa
     4381 catttcggca acagtccgaa gagagtttct aaggagtacc gttgtcaaag gcacacgggt
     4441 atgaatacgg gcaacacgat gagtgcttta gctatttgat tatttgctaa cgagtagtca
     4501 accacgcacg ctacgtaaga attatatggg gcagccattc cctgccccgc gttgttttta
     4561 ggcgatatat ttattgaaat aaataagtga catccatcac atatttatgc acttgcataa
     4621 cctgttgcat gattatttat gatctcaatt cggcattttg tcagtaaaat gcaataattt
     4681 attaaatatc aataaattag ttgtttatcg gcgagaaatt acttaataga acagaaagta
     4741 atgtcaacgc tttatggact gttatttccc tttttttagc taaatctgct atctctttat
     4801 gtgactaact tcacttacat ccacttattt ctcttcgtaa aattacttcg gaattaagta
     4861 caataagaag aggaacattt atgaagtctg cattaaagaa aagtgtcgta agtacctcga
     4921 tatctttgat actggcatct ggtatggctg catttgctgc tcatgcggca gatgatgtaa
     4981 agctgaaagc aaccaaaaca aacgttgctt t
//
LOCUS       DAJTPG010000186         4902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTPG010000186 DAJTPG010000000
VERSION     DAJTPG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..318)
                     /gene="nanK"
                     /locus_tag="OO230_003907"
     CDS             complement(<1..318)
                     /gene="nanK"
                     /locus_tag="OO230_003907"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5693470.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDA"
     gene            complement(315..1004)
                     /locus_tag="OO230_003908"
     CDS             complement(315..1004)
                     /locus_tag="OO230_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5693471.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(1052..2542)
                     /gene="nanT"
                     /locus_tag="OO230_003909"
     CDS             complement(1052..2542)
                     /gene="nanT"
                     /locus_tag="OO230_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5693472.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(2652..3545)
                     /gene="nanA"
                     /locus_tag="OO230_003910"
     CDS             complement(2652..3545)
                     /gene="nanA"
                     /locus_tag="OO230_003910"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5693473.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(3667..4458)
                     /gene="nanR"
                     /locus_tag="OO230_003911"
     CDS             complement(3667..4458)
                     /gene="nanR"
                     /locus_tag="OO230_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5693474.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1237 a         1303 c         1248 g         1114 t
ORIGIN      
        1 cgcgtcgtta atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt tgactaacgg
       61 aaagtgtagc aatccaccga gattatgcgg attcagcgcc agcaaactgc cgtcgcgaat
      121 aattccggtt gaagcgatgg caacccgctg cgcatgagct tgcaacggag agactaatgc
      181 ggataaggca tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa gttcacgacg
      241 atcgcggatc tgcccgtcag cgccaatcag cgcggcggca agtttagtac cgccgatatc
      301 aatcgccagt atggtcatag caccgccttt ttcatcgctg tgttgtacca ctgacaaatg
      361 tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg gcgcatcgca
      421 tctgccgcct gagcaggcgt gttgtaacgc ccttcggcaa tcacccgaca tccggcttcg
      481 ctcaacgttt tcaccagcgc cagatccggc tcttctggcg tttcaggcgt ggtatagcca
      541 gaaagcgtag tgccaataat ttcggctccc agcttttggc atgccaggcc gtcttccggc
      601 gttgagcagt cggtcatcgc cagtaaaccg tgatgatgga tacgtgccag cagcgtttca
      661 acaggcaccg gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc gcccgcctgc
      721 gccagcgcat cgacatcttc aatataggcc gtgatgcgta ccggagaatc ctccagatcg
      781 cgtttcacaa ttccaataat cggcacgctc accaccgcac gcgtggcttg cagatttgcc
      841 acaccttcga tgcgaatggc aaccgcgccc gcctgctctg ccgctaatgc catggctgcg
      901 acgatttcgg gtttatcgag cgggctgtca ggaaccggct ggcaggagac aatcaggcca
      961 ccgttagcag cgattttttg atccagttgt gcaagtaacg acatacatct tcccttagcg
     1021 aaaggcccgg tacatagacc gggcaacagg attaactttt ggttttgact aaatcgtttt
     1081 tggcgctgcc aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg tcatgagtac
     1141 gcaatgcttc cgggcgcagc caacgctgaa cgcgggaagg catatccaga ccaatcagca
     1201 ggatcaccac aaatgtcagg ctgaacgaga gcgatgccag cgcagtaccc agatccagac
     1261 gttgagcgat caacgcgccg atgattgggg ccagtgcgcc acccaatgcg ccaacgttgt
     1321 aggtaaagcc caggcccgct gcacgctggt cggtatcgaa ataaccgcca atcagtttcg
     1381 gtaagatccc ggcgatccct tgtccaagca tttgctggaa gaacagtaac agaccgagca
     1441 cccagacgtt tgcgccgcca atcgcaaata ccggaataat cagcagctgc gaggccagca
     1501 ggctacaaac gtacgctttg cgggttccca gccagtcacc gaggaagcca cctacgcagc
     1561 atcccacggc cgcgccaaag ccactaaaga acagcacatt ggcgacagta tgcgggttat
     1621 aagccagatc ggttttcaga tacgttggca gcagcgcctg aatcggccat gagtagagga
     1681 aagcaaacaa cacgaccacc atcagcatta cgcccgttgg ccagcgttta cccgtacttt
     1741 gcaccataaa gctgataaag attgcggcgc ataacagccc gagaacagcg acgattgcag
     1801 cattttgcag gttaccggcg aagcagaacc acagcgcagt agccgccgcc agtgtcatta
     1861 cgatattggc aatgcgatgt tcaccacggt agagaatatc caccattgtg cgtactggtg
     1921 ctttacctgc gtgtttctct ttccagtctt ccgcttccgg gatgttttta cgcagccaga
     1981 gagcaaagat gattggcaaa atgccgataa agaacagcgc acgccaaccc cagaccggaa
     2041 ccaccaggct atagacctga gcggcaacga cggcccccac agagaagcct gaaatcaaaa
     2101 aaccactggc tttgttacgc agatgttttg gccagctttc aatgacatag gtggcgctgg
     2161 aaccgtattc acccgccatc cccatgccga tgaccagacg agcgataaac atggtgatgt
     2221 agcctggcgc aaagccgcag gccagcgtcc cggccgagaa gagaacgatg ctggtgacca
     2281 ttgccagacg acgcccgtag cggtcaccca tagcgccgag catcaggccg ccgaaccagc
     2341 gagagataaa ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc ccgaattcac
     2401 cctgtacttc ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca agcagatatc
     2461 ccaaccaggc agcggaaaat gcgcgccatt gtgcacggtt gagatggcga taccacggga
     2521 tattctgggt tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc acacgctttg
     2581 gtatgaaaat tgtagggtac agatgcgttt atttcccctc acccggtagg ggcgagcgag
     2641 gggaaaacaa ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt cagttctggc
     2701 agatattttt catctaccgg tccaaacggt ttgcggcaca gcggcacaga aacgacatcc
     2761 atataatgca ggacagtttt caggccgcgg aatacgcccg ttttgatcag taaatcaatg
     2821 actttgttgc actcagtttg cagtttctgc gcggtctgga tatcgccttc tttcagcgcc
     2881 ttcacgatcc cctgatatcg ccagcccatg atgttgtaag tactgccgat accaccatca
     2941 gcgcctgcca gcaagccgga agcgaagatt tcgtcgtaac cgttatagag caccagatca
     3001 ggatgttcac gacggatctg ttccatctga tagagatcgc cagaagtctg tttcagcgca
     3061 ccgacgccag gcaacgtaac cagggtgttg atctgatcca gggtcagttt taccccactc
     3121 agggctggaa tgttgtacac caccatcggc aaaccatccg ctgaatcaat aattgcccga
     3181 tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga acggcgtgac ggcggagacg
     3241 gcatcgaagc cataacgttt agccgatgcc gcaagttgtt ggctttcggc ggtgctgacg
     3301 caaccgacgt gggcgatgag tttaatctta cctttcgcct cttcggcgac gatttccagt
     3361 acctgttcac gctcggaaag gctttgtaca aaggcttcgc cggtcgaacc acccacgtat
     3421 aaaccgtcga tgccctgctg aatattgaac tgaaccaggc ggcgcagact cgctttatcc
     3481 agtgcttgtt gttgatcaaa aggagtcagg agtgcagcca ttacgccacg taaattcgtt
     3541 gccataaata cctctgaagt gatgcttgtc tgataaacga tataccttta tacctgttat
     3601 accagatcaa ttaagcaaca ccccatacag aaagcttata atgcgatctg cttcactaaa
     3661 gtggcattat ttctttttgt tggtggtctg accgaaagcg tgccaggtag cagagacgct
     3721 gttgagatgc gattgcaacg cacgatcggc ttcgtcagga tcatgacggc ggatcgcatc
     3781 aacgatcgca atatgctgtt gataactaac gttgttatgt tcgtgcagtg cctgatcggt
     3841 aaccgttggg cgtgcggcaa taagccagtc gagcagggca acgtggatcg ccatgaagat
     3901 tgggttaccg gggatctccg ccagcacgcg gtggaaatca acgtctgaac gaatgaatgc
     3961 cgcgttgtta tccagcgact gactgttgat ttccagtgct tttgccagca aatcgatttg
     4021 ctcatcggtg gcgtgttcag ccgcatagcg caccaggctg gattcaaaga acagacgtaa
     4081 ttgttcgaaa tgggcaatcc caccaggatg agaaaggaaa tctttcgcca tgccggaaag
     4141 ctcgccgatg atagtgtccg cagaaggtcg agagacacga gcgcgttcgc cgttgtttat
     4201 ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttca cgcacagaag gacgcccgac
     4261 gttaaagaac gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc caaattcacg
     4321 acggcggatc atctgttcca gctcttcttc caccatttcg gagagttttt tacgcgccag
     4381 cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt gcgaatcaaa
     4441 tgcgttcata aggcccattc tgtaaggtca gtgtgattaa catcatcagt gacatcctat
     4501 cacaggattg aaagtagggg aaaatggcag gtttttctct ttgtgcctca tcattaccat
     4561 aattaacgga ataattaact attgcgaaaa attaatgtaa cgcagataaa cacatcccga
     4621 ttgaattatt cataagacta ttcacaagca ttatgaatat tatgaatgtg ttcttacaaa
     4681 ataatcataa tcgcatattt tttaatgaaa aatcacctca cctacaatta aaaacacgac
     4741 atccgcacca taaatagact tgcaaaaaat ataacatcat tgttttcaat ctgccattta
     4801 tgggattgac cgttttcttt tgacacggag ttcaacaatg ggtaatgctt ccaacttact
     4861 gatttagtgt atgatggtga ttttaaggtg cttgcgtggc tt
//
LOCUS       DAJTPG010000187         4861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTPG010000187 DAJTPG010000000
VERSION     DAJTPG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            187..2748
                     /gene="mutS"
                     /locus_tag="OO230_003912"
     CDS             187..2748
                     /gene="mutS"
                     /locus_tag="OO230_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5693475.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2854..3506
                     /gene="pphB"
                     /locus_tag="OO230_003913"
                     /pseudo
     CDS             2854..3506
                     /gene="pphB"
                     /locus_tag="OO230_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3557..4324)
                     /locus_tag="OO230_003914"
     CDS             complement(3557..4324)
                     /locus_tag="OO230_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5693476.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4520..4861
                     /locus_tag="OO230_003915"
                     /pseudo
     CDS             4520..4861
                     /locus_tag="OO230_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1205 a         1267 c         1246 g         1143 t
ORIGIN      
        1 tacttgcttc ataagcatca cgcaaaagct gcaaaacagc atctttcccg gaaccagcat
       61 caagaactcg ccgttcgctt cttcccctga aatgattaac tccggtatca tgtgcgcctt
      121 atgtgattac aacgaaaata aaaaccatca caccccattt aatatcaggg aaccggacat
      181 aaccccatga gtgcaataga aaatttcgac gcccatacgc ccatgatgca gcagtatctc
      241 aagctgaaag cccagcatcc cgagatcctg ctgttttacc ggatgggtga tttttatgaa
      301 ctgttttatg acgacgcaaa acgcgcgtcg caactgctgg atatttcact gaccaaacgc
      361 ggtgcttcgg cgggagagcc gatcccgatg gcggggattc cctaccatgc ggtggaaaac
      421 tatctcgcca aactggtgaa tcagggagag tccgttgcca tctgcgaaca aattggcgat
      481 ccggcgacca gcaaaggtcc ggttgagcgc aaagttgtgc gtatcgttac gccaggcacc
      541 atcagcgatg aagccctgtt acaggagcgt caggacaacc tgctggcggc tatctggcag
      601 gacagcaaag gtttcggcta cgcgacgctg gatatcagct ccgggcgttt tcgcctgagc
      661 gaaccggccg accgcgaaac gatggcggca gagctgcaac gctctaatcc ggcggaactg
      721 ctgtatgcag aagatttcgc cgagatgtcg ctgattgaag gccgtcgcgg cctgcgccgt
      781 cgcccgctgt gggagtttga aatcgacacc gcgcgccagc agttgaatct gcaatttggc
      841 acccgcgatc tggtcggttt tggcgtcgag aacgcgccgc gcggactttg tgctgccggt
      901 tgtctgttgc agtatgcgaa agatacccaa cgcacgaccc tgccgcatat tcgttctatc
      961 accatggaac gtgagcagga cagcatcatt atggatgccg cgacacgtcg taacctggaa
     1021 attactcaga acctggcggg cggtgcggaa aatacgctgg cttctgtgct cgactgcacc
     1081 gtcacgccga tgggtagtcg tatgctgaaa cgctggctgc atatgccagt gcgcgatacc
     1141 cgcgtgttgc ttgagcgcca gcaaactatt ggcgcattgc aggatttcac cgccgagttg
     1201 cagccggtac tgcgtcaggt cggcgacctg gaacgtattc tggcacgtct ggctttacga
     1261 actgctcgcc cacgcgatct ggcccgtatg cgccacgctt tccagcaact gccggagctg
     1321 cgtgcgcagt tagaaactgt cgatagtgca ccggtacagg cgctacgtga gaagatgggc
     1381 gagtttgccg agctgcgcga tctgctggag cgagcaatca tcgacacacc gccggtgctg
     1441 gtacgcgacg gtggtgttat cgcatcgggc tataacgaag agctggatga gtggcgcgcg
     1501 ctggctgacg gcgcgaccga ttatctggag cgtctggaag tccgcgagcg tgaacgtacc
     1561 ggcctggaca cgctaaaagt tggctttaat gcggtgcacg gctactacat tcaaatcagc
     1621 cgtgggcaaa gccatctggc acccatcaac tacatgcgtc gccagacgct gaaaaacgcc
     1681 gagcgctaca tcattccaga gctaaaagag tacgaagata aagttctcac ctcaaaaggc
     1741 aaagcactgg cactggaaaa acagctttat gaagagctgt tcgacctgct gttgccgcat
     1801 ctggaagcgt tgcaacagag cgcgagcgcg ctggcggaac tcgacgtgct ggttaacctg
     1861 gcggaacggg cctataccct gaactacacc tgcccgacct tcattgataa accgggcatt
     1921 cgcattaccg aaggtcgcca tccggtagtt gaacaagtac tgaatgagcc atttatcgct
     1981 aacccgctga atctgtcgcc gcagcgccgt atgttgatta ttaccggccc gaacatgggc
     2041 ggtaaaagta cctatatgcg ccagaccgca ctgattgcgc tgatggccta catcggcagc
     2101 tatgtaccgg cacaaaaagt cgagattgga cctatcgatc gcatctttac ccgcgtaggc
     2161 gcggcagatg acctggcttc cgggcgctca acctttatgg tggagatgac tgaaaccgcc
     2221 aatattttac ataacgccac cgaatacagt ctggtgttaa tggatgagat cgggcgtgga
     2281 acgtccacct acgatggtct gtcgctggcg tgggcgtgcg cagaaaatct ggcgaataag
     2341 attaaggcat tgacgctgtt tgccacccac tatttcgagc tgacccagct accggagaaa
     2401 atggaaggcg tcgccaacgt acatctcgat gcgctggagc acggcgacac tatcgccttt
     2461 atgcacagcg tgcaggacgg cgcggcgagc aaaagctacg gtctggcggt tgctgctctg
     2521 gcgggcgtac cgaaagaggt tattaagcgc gcacggcaaa aactgcgtga gctggaaagc
     2581 atttcgccga acgccgccgc gacgcaagtg gatggtacgc agatgtcttt gctgtcagtg
     2641 ccagaagaaa cttcgcctgc ggtcgaagca ctggaaaatc tcgacccgga ttcactgact
     2701 ccgcgtcagg cgctggagtg gatttatcgc ttgaagagtc tggtgtaata acaattcccg
     2761 atagtctttt gctatcggga atattaacga caactgacga atcaaataaa aataccctgt
     2821 ataataggaa agcttatttt acagggtaaa accatgccat ctacacgcta ccaaaaaatc
     2881 aatgcccatc actatcgcca tatatgggtc gttggtgata ttcatggtga atatcagtta
     2941 ttacaatccc gcttacatca actctctttt ttccccgaaa ccgacttact tatttctgtc
     3001 ggcgataata ttgatcgtgg gccggagagt cttgacgtcc tgcgcctgct aaaccagccc
     3061 tggtttatct ccgttaaagg caaccacgaa gcaatggcgc tggatgcgtt cgcgaccggc
     3121 gatggcaata tgtggcttgc cagcggtggt gactggtttt tcgatttaaa tgattgcaac
     3181 aagaggcaat agttctgttg ctgaaattcc atcaccttcc acatattatt gaaatcacta
     3241 acgacataat aaaatatgtc atcgcacatg cagattatcc gggtgatgaa tatctctttg
     3301 gcaaagaaat agcggagagc gaattactct ggcctgttta tcgtgtgcag aaatcgctta
     3361 atggcgagtt acaacaaata aacggcgctg attattttat atttggacat atgatgtttg
     3421 ataacattca gacgttcgct aaccagattt atattgatac cggatcgccg aaaagtgggc
     3481 ggctgtcatt ttataaaata aggtagtctc atgcttcttc tgtgaagcat gagtaaccag
     3541 atgttatttc aggccattat tcatttttcg ctaccagcaa agagagatcc tgcttcgcca
     3601 gcgcgcgact ggcactctcc ggcaaaccgt cgtctgtaat aatctgatca aactcgctta
     3661 atggtaacgc cagccatgtc gccacctgac catatttcgt cgcatcacag accaaaactc
     3721 gctggcggct ggcactggca atcgcccgtt tcaccgtgac tttatcttcc gctggcgtag
     3781 aaatcccccg cacactccat gacgatgcag aaataaaagc ctgatcaatc atcaggctgc
     3841 acagcatggt cgcagcggct tccccgacac aggaacggtt ttcccgacac actgcaccgc
     3901 cagtgtgaat aattgtgcaa ttactgttgt cgagcaagta gttcgcaata acgaaatcgt
     3961 ttgtgaccac agtcagtgac tccatgtgaa tcagatgctg tgctatcgct aacgtggtcg
     4021 ttcccgcatc cagatagata caacttcccg gcagaacaag acttgccgcc agcttgccaa
     4081 tagccgcttt ttgcgtcatt gccagcgcag tttttacctg atgagaaggt tcatgcgcca
     4141 cgcgtcccgg agactggacg cctccggaca ccagcacaac ggctccctgc tgctccagtt
     4201 tttgtaaatc ccgacgaatg gtcatatgtg acacattaat tctgtccgtt agttcagcaa
     4261 tactgacaat gcctttttca gctaccatct caaggatgat ttggcgacgc tctacgggta
     4321 tcaacttttg ctccttcctt tgtcctgctg acattctacg ctatttgcct gcgaaacgtg
     4381 cgcggcgcaa ctaacgctta gttcacataa aataacacaa aatgttaatt tatgtgaatc
     4441 atctcaccat atcgttatct cccagcgctt atgttcacaa tatcaaacaa aatatcactt
     4501 aaattaacaa ggagagcaga tgaaaacggg atctgagttt catgtcggta tcgttggctt
     4561 agggtcaatg ggaatgggag cagcactgtc atgtgtccgc gcaggttttt ctacctgggg
     4621 cgcagacctg aacagcaatg cctgcgctac gttgaaagag gcaggtgctt gcggggtttc
     4681 tgataacgcc gcgacgtttg ccgaaaaact ggacgctctg ctggtgctgg tggtcaatgc
     4741 ggcctaggtt aaacaggtgc tgtttggtga aacaggcgtt gcacaacatc tgaaacccgg
     4801 tacggcagta atggtttctt ccactatcgc tagtgctgat gcgcaagaaa ttgctaccgc
     4861 t
//
LOCUS       DAJTPG010000188         4730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTPG010000188 DAJTPG010000000
VERSION     DAJTPG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            219..4337
                     /gene="pic"
                     /locus_tag="OO230_003916"
     CDS             219..4337
                     /gene="pic"
                     /locus_tag="OO230_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5693477.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4488..>4730)
                     /locus_tag="OO230_003917"
     CDS             complement(4488..>4730)
                     /locus_tag="OO230_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR5693478.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1271 a         1073 c         1250 g         1136 t
ORIGIN      
        1 gaagcagaaa aagcttatta tgcttccatc ggaaatgatg atctggcagc ctgagttcac
       61 agataaaaca ctctccagga aacccggggc ggttcagttc acaaaaacac attaatgcag
      121 taactatatt ttcctttctg gtgataacgt cgggttatca ttagcttctt cagctatttt
      181 acttttatat cccttgtaaa catcatggag aatccatagt gaataaagtt tattctctta
      241 aatattgccc cgtcaccggg gggcttattg ctgtctctga acttgcccgc agggtaataa
      301 aaaagacatg ccgaagatta acgcatattc ttctggctgg cattccagca atctgtctgt
      361 gttactctca gatatcccag gcgggtattg tccgttccga tattgcctat cagatttatc
      421 gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc caatgatatt ccggtatatg
      481 ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc aatggccgat ttcagcagtg
      541 tgagctcaaa tggcgttgct acgcttgtat cgcctcagta tatcgtcagc gtaaagcata
      601 acggaggata tcggagtgtg agctttggta atgggaaaaa tacatattcc cttgttgacc
      661 gtaataacca cccttctatt gacttccatg ctccacgtct gaataaactg gttacagaag
      721 ttattccctc agcggtaaca tcagaaggaa ccaaagccaa tgcttataaa tacactgaac
      781 gttacaccgc tttttatcgg gtgggtagtg gtacgcagta cactaaggac aaggacggaa
      841 atttagttaa ggttgccggt ggatatgctt ttaaaacagg aggaaccaca ggagttcctc
      901 tgatatctga tgcaacaata gtctctaatc ccgggcaaac ttataatcct gtaaacggcc
      961 ctttacctga ctatggagcc cctggggata gtggttctcc tttgtttgct tatgataaac
     1021 aacaaaaaaa atgggttatt gttgctgtat taagagcata tgcaggtatt aatggtgcta
     1081 cgaactggtg gaatgtcata ccaacagatt atctgaacca ggttatgcag gacgatttcg
     1141 atgcccccgt agactttgtt tccggactgg gccccctgaa ctggacatac gacaaaacat
     1201 caggcacagg taccctgagc cagggcagta aaaactggac catgcacggg cagaaagaca
     1261 atgacctcaa tgccggtaaa aatctggtat tcagcgggca gaatggtgca attatcctga
     1321 aagacagtgt gactcagggt gccggttatc tcgaatttaa agacagttac accgtatctg
     1381 ctgaatccgg aaaaacatgg acgggtgccg gcattattac tgacaagggg acgaatgtaa
     1441 cctggaaggt caacggcgtt gccggtgaca acttgcataa gctgggggaa ggaaccctga
     1501 ccataaacgg aacaggtgta aacccgggag gactgaaaac gggagacggt atcgttgtac
     1561 ttaaccagca ggcagacact gcaggtaata tccaggcctt cagttcagtg aacctcgcca
     1621 gcggacgtcc gaccgtggtg ctcggggatg cccgtcaggt caatccggat aacatttcat
     1681 ggggataccg gggaggtaag cttgacctta atggtaatgc cgttaccttc acccgactgc
     1741 aggctgctga ttacggggcg gtgattacaa ataatgcaca gcaaaaatcc cagcttttac
     1801 tggatcttaa ggctcaggat acaaatgtca gtgaaccgac gattggaaat atatccccct
     1861 ttggtggtac cggcactcca ggaaacctgt acagcatgat actcaacagc cagacccgct
     1921 tctatattct gaaatctgcc agctatggta acactctgtg ggggaacagc ctgaatgatc
     1981 cggctcagtg ggagtttgtt ggcatgaaca aaaacaaagc agttcagaca gtaaaagata
     2041 ggatcctggc cgggcgggca aaacaacccg ttatctttca tggtcagctg accgggaata
     2101 tggatgtcgc cattccacag gtgccggggg gaagaaaggt aatctttgat ggtagcgtga
     2161 acctgccgga aggtaccctg agtcaggaca gtggcaccct gatattccag ggacatccgg
     2221 ttatccatgc ctccatcagt ggcagtgcac cggtcagcct gaaccagaaa gactgggaaa
     2281 accgtcagtt tacaatgaaa acactgtcgc tgaaagacgc tgacttccat ctttcacgta
     2341 acgcctcgct gaacagtgac attaagtcgg ataacagcca tatcacactg ggaagtgaca
     2401 gggcatttgt ggataaaaat gacggaacag gaaattatgt cattccggag gaaggtacct
     2461 ctgtcccgga caccgtgaat gacaggagcc agtatgaagg gaatattacg ctgaaccata
     2521 actcagccct ggatatcggc agcaggttca ccggggggat tgacgcttat gacagtgccg
     2581 tcagcatcac ctctccggac gtcctgttga cagccccggg tgcttttgcc ggcagttcac
     2641 tgacagtgca tgatggcggt catcttacag cactgaacgg tcttttcagc gacgggcata
     2701 ttcaggccgg taagaacggc aaaatcaccc tgagcggtac accggttaaa gatacggcta
     2761 atcagtatgc ccctgctgta tatctgacgg acggatatga cctgaccggc gataacgcag
     2821 cactggaaat tacccgtgga gcacatgctt ccggtgatat tcatgcctct gcggcatcaa
     2881 cagttaccat cgggtctgac acgccggcag aactggcttc tgcggaaacg gctgcatcgg
     2941 cgtttgccgg cagtcttctt gagggctata acgcagcatt caatggtgcc ataaccggtg
     3001 gcagggctga tgtcagtatg cataatgcac tgtggactct gggtggggac tctgccatcc
     3061 acagtcttac cgtcagaaac agccgtatta gttctgaagg agaccgtaca ttccgtaccc
     3121 tgacggtgaa taaactggat gcaacaggca gtgattttgt tttgcgtacg gacctgaaaa
     3181 atgccgataa aattaatgtg actgaaaaag ccactggttc agataacagc ctgaacgtca
     3241 gctttatgaa taatcctgct cagggacagg ccctgaatat tcctctggtc acggcaccgg
     3301 cgggaacttc agcagagatg tttaaggccg gcacccgggt gacaggtttc agtcgggtga
     3361 ccccaaccct gcatgttgat accagtggtg gcaatacgaa gtggatactg gatggtttta
     3421 aagcggaggc tgataaagcc gctgccgcga aggctgacag tttcatgaat gccgggtata
     3481 aaaacttcat gacggaagtt aacaatctga acaaacgtat gggtgacctg cgtgacacaa
     3541 acggtgatgc cggtgcctgg gcgcgcatca tgagtggtgc cggttctgca gacggtggtt
     3601 acagtgataa ttacacccat gttcaggtcg gctttgacaa aaaacatgaa ctggacggtg
     3661 tggacctgtt taccggtgtc acgatgacct ataccgacag cagtgcagac agccatgcat
     3721 tcagcggaaa gacgaaatcg gtggggggcg gtctgtatgc ttcagcattg tttgagtccg
     3781 gtgcctatat cgatttgatt ggtaaatata ttcaccatga caatgattac acaggtaact
     3841 ttgctagcct gggaacgaaa cactacaaca cccattcctg gtatgccggt gctgaaacgg
     3901 gttaccgcta tcacctgaca gaggacacgt tcattgagcc gcaggctgaa ctggtttacg
     3961 gcgccgtgtc cgggaaaaca ttccgctgga aagacggtga tatggacctg agcatgaaga
     4021 acagggactt cagtccgctg gttggaagaa caggggttga actgggcaag accttcagtg
     4081 gtaaggactg gagtgtgacg gcccgtgccg gaaccagctg gcagtttgac ctgctgaata
     4141 atggagagac cgtactgcgt gatgcgtccg gggagaaacg gataaaagga gagaaggaca
     4201 gccggatgct gtttaatgtt ggtatgaatg cgcagataaa ggacaatatg cgctttggtc
     4261 tggagtttga gaagtcagcc tttggtaaat ataacgtgga taatgcggta aacgcgaatt
     4321 tccggtatat gttctgatgt gggggtaaag ggctgccgga aggcagcctt tctcagagac
     4381 tcttaaaata taaaactgaa ggtcttcggt aagggggagt gtattagacg tcaactagtt
     4441 ttgccgactg gcgcgagcct gtttaagatt ctgtgtaaat gcctttctca gaagtgaccg
     4501 tccaggcggt caccgaactc gataataaag cggctcattg ccattcgcca gtccttcaac
     4561 ggcatcgtcc atttctggga cgcagactgg attgccagcc acaccacttt tttcaccgag
     4621 tcgtctgtcg ggaacacttt acgctttttg atcgcatggc ggatcacgct gtttagcgac
     4681 tcgatggcat tcgtcgtata gatcactttg cggatgtccg ttggataagc
//
LOCUS       DAJTPG010000189         4621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTPG010000189 DAJTPG010000000
VERSION     DAJTPG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..370
                     /gene="bglB"
                     /locus_tag="OO230_003918"
     CDS             <1..370
                     /gene="bglB"
                     /locus_tag="OO230_003918"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5693479.1"
                     /translation="RVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLN
                     DHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTR
                     KKSFGWYAEVIKTRGLSLKK"
     gene            456..2072
                     /gene="bglH"
                     /locus_tag="OO230_003919"
     CDS             456..2072
                     /gene="bglH"
                     /locus_tag="OO230_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5693480.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2099..3274
                     /locus_tag="OO230_003920"
     CDS             2099..3274
                     /locus_tag="OO230_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR5693481.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3276..3998
                     /locus_tag="OO230_003921"
     CDS             3276..3998
                     /locus_tag="OO230_003921"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5693482.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4060..>4621)
                     /locus_tag="OO230_003922"
     CDS             complement(4060..>4621)
                     /locus_tag="OO230_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5693483.1"
                     /translation="TFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVDEVE
                     HLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERT
                     PGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCL
                     QKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1282 a         1021 c         1128 g         1190 t
ORIGIN      
        1 acgggttctg ttaaatacgc tttgggatcg ttatcaaaaa ccgttattta ttgtcgagaa
       61 tggattaggc gtaaaagaca gcgttgaagc ggatggttcg atacaggacg attatcgaat
      121 tgcctattta aacgatcacc tggtacaggt aaatgaagcg attgccgatg gtgtggatat
      181 tatggggtac accagttggg ggccaattga tttagtcagt gcatctcatt cacaaatgtc
      241 taagcgctac ggctttattt atgtggatcg tgatgataat ggcgaaggaa gcctcacaag
      301 aacgcgtaag aaaagcttcg gatggtatgc agaagtgatc aaaacgcggg ggctgtcatt
      361 aaaaaaataa ccaataaagc accttaatta tcgtcgcatt cagaacagtc tggatgcgat
      421 acgttaattc tttctttgca ccataaaggg atattatgtt tagacaaaat cttattacct
      481 ctgccatctt attaatggca ccgttagcct tttctgcaca atcattggct gaatcattaa
      541 cggtggaaca acgccttgag ttattagaaa aagcgttaag agaaacgcaa agcgaactca
      601 aaaagtataa agatgaagag aagaaaaaat atacgccagc gacggtgaat cgtagcgtaa
      661 gtacgaatga tcaagggtat gccgccaatc cgttcccgac cagtagtgcc gcaaaacctg
      721 atgctgtact ggtcaaaaat gaagagaaaa atgccagtga gacaggctcg atttattctt
      781 ccatgactct gaaagatttc agtaagtttg tgaaagatga aattggcttt agttacaacg
      841 gctattaccg ttctggttgg gggaccgcct ctcatggttc acctaaatca tgggcgattg
      901 gttctctggg ccgctttggt aacgaatact ccggctggtt tgatttgcag ttaaaacaac
      961 gtgtctacaa cgaaaacggc aaacgggttg atgccgttgt gatgatagat ggtaacgttg
     1021 gtcagcagta ctctaccggc tggtttggcg ataatgccgg tggcgagaac tttatgcagt
     1081 tctccgatat gtacgttacc accaaaggtt tcctgccctt tgcgccagag gctgatttct
     1141 gggtgggtaa acacggtgcg ccgaaaattg aaatccagat gctggactgg aaaacgcagc
     1201 gtactgatgc tgcagcgggt gtaggtctgg aaaactggaa agtcggtccg ggtaaaattg
     1261 atatcgcgct ggttcgcgaa gatgtcgatg attacgatcg cagcctgcaa aacaaacagc
     1321 agattaatac ccatactatt gatttacgct ataaagatat cccgttatgg gataaagcga
     1381 ccttaatggt gagtggtcgt tatgtcacgg caaacgaaag cgcatcggaa aaagataatc
     1441 aggataataa cgggtattat gactggaaag atacctggat gttcggcaca tctttaacgc
     1501 agaaatttga taaaggtggc ttcaacgaat tctccttcct ggttgcgaat aactctatcg
     1561 ccaggaactt tggccgttat gctggcgcaa gtccatttac cacctttaat ggtcgttatt
     1621 atggtgatca caccggcgga acagcagttc gtctgacctc gcagggcgaa gcctatatcg
     1681 gcgatcattt tattgtggct aacgcgattg tttactcctt cggtaacaat atttatagct
     1741 acgaaacagg cgcacactct gatttcgaat ctattcgtgc ggttgttcgc ccggcctata
     1801 tttgggacca atataaccag acaggtgttg aactgggcta tttcacccag caaaacaaag
     1861 atgcgaatag taataaattt aatgagtctg gttataaaac cacgctcttc cataccttta
     1921 aagtcaatac cagtatgttg acctcgcgtc tggaaattcg tttctacgcc acgtatatca
     1981 aagccctgga aaacgaactg gatggcttca ccttcgaaga caataaagac gcccagtttg
     2041 ctgtcggtgc ccaggctgaa atctggtggt aatggaactt aatgaaagga atattacgat
     2101 gaatataaaa attgctgctt taacgctggc tattgccagc ggtatttctg ctcagtgggc
     2161 catagcagcg gatatgccag ccagcccggc acccactatc ccggtgaaac agtatgtgac
     2221 tcaggtcaat gccgataaca gcgtgacctt tcgctacttt gcccctgggg caaaaaatgt
     2281 ctctgtagtg gtgggtgttc cggttccgga caatattcac ccgatgacca aagacgaagc
     2341 aggagtctgg tcgtggcgca cacctatcct gaaaggcaac ctgtacgagt attttttcaa
     2401 tgttgatggt gtacgcagca ttgatacagg caccgcaatg actaagcctc agcgccaggt
     2461 taactccagt atgattctgg tgccaggcag ttatctggat acgcgttctg ttgcgcatgg
     2521 tgatttgatc gccataactt accactccaa cgcattgcaa tctgaacgtc agatgtatgt
     2581 ctggaccccg ccaggataca ccggcatggg cgagcctttg ccagtgctct atttctatca
     2641 cggttttggt gataccggac gttccgctat cgatcagggg cgtatcccgc aaatcatgga
     2701 taacctgctg gctgaaggga aaattaaacc gatgctggtg gtgatcccgg ataccgaaac
     2761 cgatgcgaag ggcattattc ccgaagattt cgtgcctcag gaaagacgta aagtctttta
     2821 tccgctgaat gctaaagcgg cagatcgcga actgatgaac gatattatcc cgctgattag
     2881 caagcgtttt agtgtccgta aagatgccga tggtcgcgcg ctggcagggc tttcacaagg
     2941 cgggtaccag gcgctggttt ccggaatgaa tcatctggaa agctttggct ggctggccac
     3001 attcagtggt gttaccacga caaccgtacc ggatgaaggt gtcgcggccc ggctgaacga
     3061 tccggcagct attaaccagc aactacgtaa ttttactgtg gtcgttggtg ataaagatgt
     3121 cgtaaccggc aaggatatcg ccgggctgaa aactgagctt gagcagaaaa aaattaagtt
     3181 tgattaccag gaatatcccg gcctgaacca tgaaatggat gtctggcggc ctgcctatgc
     3241 agccttcgta cagaaattta aaataaggca ttaagatgaa attaatcatt accgaagatt
     3301 accaggaaat gagccgtgtc gcggcacacc atctgcttgg ttatatgtcg aagacgcgtc
     3361 gtgttaacct ggcaattacc gccggtagca cgcccaaagg catgtatgaa tacctcacca
     3421 ccctggttaa aggtaagcct tggtacgata actgctattt ctataatttc gatgaaattc
     3481 cctttcgcgg caaagaggga gaaggcgtaa cgattaccaa tctgcgtaat ctgtttttca
     3541 cccctgcggg gatcaaagaa gagaatatcc agaagctcac tattgataac taccgcgagc
     3601 atgatcagaa actggcgcgt gaaggcggcc tggatttggt ggtgctggga ttaggtgcag
     3661 atggtcattt ttgtggcaat ttgccgaata cgacccattt ccatgagcaa accgtggagt
     3721 tcccgatcca gggagagatg gttgatattg tggcgcatgg tgagttgggc ggcgactttt
     3781 cgttagtgcc tgacagctac gtgacgatgg ggccgaaaag catcatggcg gcgaaaaact
     3841 tgctgataat tgtcagtggc gcaggtaaag cgcaggcact gaaaaatgtg ctgcaaggtc
     3901 cggttaccga agatgttcca gcttccgtgt tgcagctgca tccttcgctg atggtaatcg
     3961 ctgataaagc cgctgccgca gaattagcgt tcggttaagc tgaagaaata aggccggatg
     4021 tatgaattac gctccggcct gttctcatta tttaaataac tacgaaaaat caccccacag
     4081 tctcagcttg ccgcagtgga gacagcggaa gagataaccc tgacaatcac cacccttttg
     4141 caggcattta gctagatcag aatttctaat accgaaattc tcacaatctt cttcaaggtt
     4201 ggcaaattca tcgaggcgat cttttatatc attccagccc acatagccga taaaagcaca
     4261 gaaatcgcca caatgcgcaa gccagaattc ctgttgccat ccatgataac ctggcgtgcg
     4321 ttcaaccaac tctttcagca tttcatcagg atatgtgttc ttaataccgg caaattcgtc
     4381 ctcttcatca tactcaaatt caacaccttc tatgctggca tcatcctgaa aactacctgc
     4441 aaacttttct gcagcagaac cgtccgcaat acacaacgga cagagatgtt caacttcatc
     4501 aacgcaataa aagggacccg agtaataaac tgacgtctgt tgttcacagc aatcgcactc
     4561 tacggtttta tcctgttcaa atgcgcctgt ttccagtggc ttgggatgat atttgaatgt
     4621 a
//
LOCUS       DAJTPG010000190         4602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTPG010000190 DAJTPG010000000
VERSION     DAJTPG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO230_003923"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO230_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5693484.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO230_003924"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO230_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5693485.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO230_003925"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO230_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5693486.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /locus_tag="OO230_003926"
     CDS             complement(3929..>4602)
                     /locus_tag="OO230_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5693487.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAJTPG010000191         4593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTPG010000191 DAJTPG010000000
VERSION     DAJTPG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            190..447
                     /locus_tag="OO230_003927"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO230_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO230_003928"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO230_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5693488.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO230_003929"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO230_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5693489.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO230_003930"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO230_003930"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5693490.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO230_003931"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO230_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO230_003932"
     CDS             complement(3605..4420)
                     /locus_tag="OO230_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5693491.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>4593
                     /locus_tag="OO230_003933"
     CDS             4517..>4593
                     /locus_tag="OO230_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5693492.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFI"
BASE COUNT         1192 a         1063 c         1082 g         1256 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctg
//
LOCUS       DAJTPG010000192         4558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTPG010000192 DAJTPG010000000
VERSION     DAJTPG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            278..457
                     /locus_tag="OO230_003934"
                     /pseudo
     CDS             278..457
                     /locus_tag="OO230_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(487..969)
                     /locus_tag="OO230_003935"
                     /pseudo
     CDS             complement(487..969)
                     /locus_tag="OO230_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1225..2205)
                     /locus_tag="OO230_003936"
     CDS             complement(1225..2205)
                     /locus_tag="OO230_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5693493.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(2205..3404)
                     /locus_tag="OO230_003937"
     CDS             complement(2205..3404)
                     /locus_tag="OO230_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5693494.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(3726..4064)
                     /locus_tag="OO230_003938"
                     /pseudo
     CDS             complement(3726..4064)
                     /locus_tag="OO230_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4131..>4558)
                     /gene="istB"
                     /locus_tag="OO230_003939"
     CDS             complement(4131..>4558)
                     /gene="istB"
                     /locus_tag="OO230_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5693495.1"
                     /translation="AIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGV
                     MAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAAL
                     TSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1226 a         1065 c         1007 g         1260 t
ORIGIN      
        1 gagtactacg gaaagttctg gtccagaaat ttcaactatc gactgtggag tgcaatgcag
       61 tcacgtctgc tcaagtggat gcagtctaaa tacagacttt cgaaccggaa ggctcagcga
      121 aagctgacgc tggtaaggaa ggagtatccg aagctatttg ttcactggta tttactacgt
      181 gcatcaaatg agtgatcaag agccgtatga cgcgagagtg tcacgtacgg ttccgtgagc
      241 agccggggag tgaaattctc tccggctgac tcgactcgcg ggttccgaca gcgatgacga
      301 acatgcggcg gtgttgtact cgctgatcgg cacatgccgt ctgaacaatg tggaaccaga
      361 aaaatggctg cgttacgtca ttgagcatat ccaggactgg ccggcaaacc gggtacgtga
      421 tctgttgccc tggaaagttg atctgagctc tcagtaaata tcaatacggt tctgacgagc
      481 cgcttacttt tggcgtaatt ctgctgcgcc tttactgaac acaatgccgt attcagcaag
      541 aagtccacga atgtgattta tcagttgtgt cctttccatt gaaaccaacc gacgcatatt
      601 caaaaaagtc tggacgtcct gttgctccag agtttttatt ggtacgggtc gtatagttcc
      661 atgttggcag gcctcagcta ttgcctgtgc gtcgttataa tcatttttct ggcaccgctg
      721 ataagcttta acatgttgtg ccggaagcaa tattacatca tggtcgagtt tcctgatttc
      781 ccgggcccag taatgagatg ccccgccagc ctccatcgca acagtcattt taggctgttg
      841 caccagccag ttaagcagtt gattgcgaga aagtttggtc tttcctgcgg gtttgccatg
      901 agcattgata ctgaaaagat aaaagttcgt tttagccaga tcaatgccgg tgattttctg
      961 ttcagacatg gtatcccgct tacttttgtt gttaaagttt atccatcatg gcgcaattga
     1021 aggctgccgt gaaagagggg gaagactatc tcatcggtta ctgttaaacg gctaagagaa
     1081 actatctttt tcaagcaaag aagatacggt gaaacaggcg atttcaccgt gaaatactgt
     1141 tcaaaaaaaa cgctaataaa agcaaaattt cttttaaaac agccaattaa aaactggcga
     1201 gttgctggcg aaaaacttga attttcacag cgaattcaga ttatctttta gtacaagagc
     1261 aatcatacga tctaacttat cctgaacttc caacggtaat ctgccgaatt catatgagaa
     1321 cgtacgtccc tttactcgtt ttcgagcaaa tttatcttta ctatcaaatt tccataacaa
     1381 agttacaacc gctttatcct ttactcctga atcaagcaat gaagacattt ccttggttat
     1441 aagccgcata atactgtttt taacctcgtc ttcagaaaga ttgtcatcag cagtaataag
     1501 ctcaatactc ggcgagatat tcttcacaac ttcatcaata gatatatcac ccagcctcag
     1561 tcgttcactt aattcagcca gttgcctgta atcggcgtat gtcagctcag actgaacagg
     1621 aaaaagagac acaaaatctt taggtacgga cgctgcttgg agggcacgag tcaccttagc
     1681 tgccgacaat ccctctcttt ccgcaatttg cttctggttc ataccggcct ctttgagacg
     1741 tactaaacgc aacccaattt cacgaatatt atgttcaagt gacgtctgca aatctttcgc
     1801 cagatgctga gcttcagaaa cagtaagctc atcatccgtt acaagtacac gtaatccaac
     1861 cttgcatagc aaagctgctg cccgacgacg ggaaccatcc aaaatctcaa taagatcgcc
     1921 agtccgaatc ccaatacatg gatagaattg ttgtaaacgt atagttctgg tgatgtcttt
     1981 tagtgagtcc ggcgtcaggg ctgtctgttc gcggccattc acctcttcaa ccacaaaagt
     2041 ttgagtatcc actttgtcat gatcaacctt gatctcagta aattttgcct ttcggccagt
     2101 attgagggta aatacatgaa ctggagccga tatttcttca gtattattca ggatatttgt
     2161 attcagcgta cggccaatag tcggtcggtg ctttctgttt tccatcagta gttggccctt
     2221 ataaattcaa tacgatcaaa aactgcttta gcgaaatctt ctgctgccat acgtgcattt
     2281 ttcaaagctt caccactgcc aacataagta acaggattcg cagatatcac ggtatcaaac
     2341 gactcgccgc ttctctcaaa accatcaaga cgtggcattg aaacatcaag catgtcacca
     2401 ccgaaaatct cttttgtcag gctatggcag tatttatgat ctgacttatt cgccagtttc
     2461 gacatgaagc caatatttgc ctgcaaacgg catgaacatc catcctgttc aataatctgt
     2521 acaagttcag gtaaacgagc caaatattta agcgtggaat ggaaatcaac ttgtgctggc
     2581 ggcaccggtg taaacatgat atctgcggca gcaatggcat ttttcaaaaa tgcgtccaga
     2641 tgcgggcctg tatcaataag gatgaagtcg aaatcatgct tcagcttatc aataatattc
     2701 tcacgcaata tcgcgtgttt gttttgtcct aatagatgct cctcgcacag agtatcccag
     2761 tttgatgcaa taaaggcgtc gtcaatagaa gccggcataa cataaacgcc agggataact
     2821 gacggaacga taaaatcttc cagtaattcc tcacgactca cgttctgcaa catagcctgc
     2881 ggtgcggttg tatcaaccag ccccaccgca tgcaagtagt tcagaaacat agtggctgat
     2941 gactggggat ctaaatcaag gagcagaata cgtaaatctt ctgacagcag gtgaggatga
     3001 gcacgcaggg cgtgagctac ggaaacacta ctcacggttt tagacacacc gccttttaga
     3061 ctaccgatga atatggaata tgcctcacta taacgatcgc gatatttagg aatgccacga
     3121 tgtgcataga tatcgataat gttctggatg gttaatgcat attttgtcgc ggttcctgct
     3181 gggcgttttt caaactgata gccctgagcc tccatctcac cgactgcacc atccacgctt
     3241 gcgcgcgtta attttggaag cttagctaca gcagactttg aatagacttg ataaaaaaca
     3301 tcagcctgaa actcctgctt ttgctcctga atctgttttg ttagggcgag caacatttta
     3361 tctgcacgct gcgcaacctt acttaactgc tcaaaactag tcattccccc tccgtgctat
     3421 gtaggatttt aacttattac aaaaaaaact gcattagcag ggtaatgcat gcaagtacat
     3481 attgtacatg cttatattaa aaatgcgcgc attgctgatt tattaatcat cggaatgaat
     3541 attactgtaa gatagtatgc ctttccggga gaaagacgct gatttccccc gaataatcca
     3601 gttcaccacc acactccgga caacagcttt cttcaggctc cagatggtgt atttcgcggg
     3661 aaacatgttc cggtaacgga cggcgatgac gtgactgtcg caacaggcgg gggtagtgct
     3721 tccaatcagt agaacatgtg cttttcaatg aaggagccga tgactttttc gtggatttcc
     3781 acggaacgcg agaagcagat tgttttacgt gccagccgct tgatgcgggt gcggagggtc
     3841 cggttgttac gtgcaatacg ttgcgtgaag attttgccgg tcaggtgttt ctccttcggc
     3901 acttctctgg cgtagctgcc ccagtcatcg ctggtcacca tgccgataca gaacggagtc
     3961 aacagggcta acagttcccg acaggtctca tcattccgtg gcccgaaagt gtaagccaac
     4021 acaccgccag ttttagtgtt gtacgcatac cagagccagt gttgtgtcaa cgacggatga
     4081 aaagtgatcc acttatatct ccaccaacgg cccaatattg atccaccgtt ttactcagga
     4141 ttagcttctg ctataacccc ggcctttcgt ttctgtctga gtcgatagct ttctcctttg
     4201 atttgaacga catgtgagtg gtgtaagata cggcccagca tcgctgaggt cagtgctgca
     4261 tcaccggcga acgtttgatc ccactgcccg aacggtagat tggatgtcag gatcattgcg
     4321 ctcttttcgt aacgtttagc gatgacctgg aagaacagtt ttgcttcttc ctgactgaac
     4381 ggcagatagc ctatttcatc aatgatgagc aggcgggggg ccattactcc acgctgaagc
     4441 gtcgttttat aacggccctg acgttgtgcc gtagataact gaagtaacag atctgctgct
     4501 gttgtgaagc gaactttgat acctgcacgg actgcttcat agcccatcgc tattgcca
//
LOCUS       DAJTPG010000193         4553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTPG010000193 DAJTPG010000000
VERSION     DAJTPG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..285
                     /gene="ydcF"
                     /locus_tag="OO230_003940"
     CDS             <1..285
                     /gene="ydcF"
                     /locus_tag="OO230_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein"
                     /protein_id="HCR5693496.1"
                     /translation="APRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGEL
                     PRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            482..1921
                     /gene="aldA"
                     /locus_tag="OO230_003941"
     CDS             482..1921
                     /gene="aldA"
                     /locus_tag="OO230_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5693497.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(1963..2963)
                     /gene="gap"
                     /locus_tag="OO230_003942"
                     /pseudo
     CDS             complement(1963..2963)
                     /gene="gap"
                     /locus_tag="OO230_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3152..3682
                     /gene="cybB"
                     /locus_tag="OO230_003943"
     CDS             3152..3682
                     /gene="cybB"
                     /locus_tag="OO230_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5693498.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3927..4100
                     /locus_tag="OO230_003944"
     CDS             3927..4100
                     /locus_tag="OO230_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693499.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4172..4321)
                     /gene="hokB"
                     /locus_tag="OO230_003945"
     CDS             complement(4172..4321)
                     /gene="hokB"
                     /locus_tag="OO230_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5693500.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4422..>4553)
                     /locus_tag="OO230_003946"
     CDS             complement(4422..>4553)
                     /locus_tag="OO230_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5693501.1"
                     /translation="SNISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV"
BASE COUNT         1157 a         1035 c         1136 g         1225 t
ORIGIN      
        1 gcaccacgct ggttaagtta tcccggattc gttcctcagt taggaaataa cgcagacagt
       61 gtaatcttta ttaatcagtt acaaggatta tggccggttg agcgttatct ctcactactc
      121 actggcgagc tgccgcgttt acacgatgat agcgatggct acggtccccg cgggcgagat
      181 tttatcgttc acgttgattt tccggcagaa gtcatccatg catggcaaac gctgaaacat
      241 gatgcggtgc tcatcgaggc gatggaaagt cgctcgttac gttaaaaatt gcccgtttgt
      301 gaaccacttg tttgcaaacg ggcatgactc ctgactttta tttctgcctt ttattccttt
      361 tacacttgtt tttatgaagc cctccacaga cttgtccttt cccgatgccg tctctctgat
      421 gattgatgtt aattaacaat atattcaccg aaaacaaaca tataaatcac aagagtcgcc
      481 catgtcagta cccgttcaac atcctatgta tatcgatgga cagtttgtta cctggcgtgg
      541 agacgcatgg attgatgtgg taaaccctgc tacagaggct gtcatttccc gcatacccga
      601 tggtcaggcc gaggatgccc gtaaggcaat cgatgcagca gaacgtgcac aaccagaatg
      661 ggaagcgttg cctgctattg aacgcgccag ttggttgtgc aaaatctccg ccgggatccg
      721 cgaacgcgcc agtgaaatca gtgcgctgat tgttgaagaa gggggcaaga tccagcagct
      781 agctgaagtc gaagtggctt ttactgccga ctatatcgat tacatggcgg agtgggcacg
      841 gcgttacgag ggcgagatta ttcaaagcga tcgtccggga gaaaatattc ttctgtttaa
      901 acgtgcgctt ggtgtgacta ccggcattct gccgtggaac tttccgttct tcctcattgc
      961 ccgcaaaatg gctcccgctc ttttgaccgg taataccatc gtcattaaac ccagtgaatt
     1021 tacgccaaac aatgcgattg cattcgccaa aatcgtcgat gaaataggcc ttccgcgcgg
     1081 cgtgtttaac cttgtactgg ggcgtggtga aaccgttggg caagaactgg cgggtaaccc
     1141 aaaggtcgca atggtcagta tgacaggcag cgtctctgca ggtgagaaga tcatggcgac
     1201 tgcggcgaaa aacatcacca aagtgtgtct ggaattgggt ggtaaagcac cggctatcgt
     1261 aatggacgat gccgatcttg aactggcagt caaagccatc gttgattcac gcgtcattaa
     1321 tagtgggcaa gtgtgtaact gtgcagaacg tgtttatgta cagaaaggca tttatgatca
     1381 gttcgtcaat ctgctgggtg aagcgttgca ggcggttcaa tttggtaacc ccgctgaacg
     1441 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
     1501 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
     1561 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggaaa tgtcgattat
     1621 gcatgaggaa acctttggcc cggtgctgcc agttgtcgca tttgacacgc tggaagatgc
     1681 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
     1741 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
     1801 aaacttcgaa gctatgcaag gtttccacgc cggatggcgt aaatccggta ttggcggcgc
     1861 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtctta
     1921 atgagtgaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1981 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     2041 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacactga
     2101 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     2161 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     2221 ccgagaatgg acaccagctc agtgaccgaa cctgttttca ccggcacgcg ttgcgcatga
     2281 cctttcagtt tgccgctcag ttccgggatc accagaccaa tggcttttgc cgcccccgta
     2341 gtgtggggaa tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt accacgcggg
     2401 ccatccacca gtgactgggt gccagtatag gcatgaatgg tcgtcatcgt gccgacttct
     2461 atcccgaaac tgtcatgcaa ggctttggcc atcggcgcaa ggcagttagt ggtgcatgac
     2521 gccacggaaa caatggtgtc gttgccatcc agagtgtcgt cattaacgtt ataaacgatg
     2581 gttttcattt caccggcagg agcggaaatc aacaccttct tcgcaccagc atcaagatgc
     2641 gcctgcgatt tatcggcgga ggtataaaag ccagtacatt cgacaatgat ttctgcccct
     2701 ttcgctttcc acggaatatt tttagcctct ttttcggcgt aaaccgcgat acttttccca
     2761 tcaacgataa gtgaatcttc cgtaaaatca acgctccagg ggaacggacc atagtttgaa
     2821 tcatgtttca gcagataggc gagaattttt ggggaagtaa gatcattaat agcgacaacg
     2881 tctatgttgc ttttgacttc aagtaaacga cgcaacacca gtcgaccgat acgaccaaaa
     2941 ccgttaatac caactttact catggttttc tcctgtcagg aacgttcgga tgaaaattga
     3001 tcctttccag gcttagacca ggattgtggg atgggcaatc tccattctta cagtgaaacg
     3061 taacgtactg aaaacgggtg aacaatattt aatgaaattt tgagaaaagc ccgttatgtt
     3121 aacggaaaat tatgttaaag caggaaatgt tatggaaaat aaatactcaa ggttacaaat
     3181 cagcattcac tggctggtct ttttgctggt tatcgcagcg tattgcgcaa tggagtttcg
     3241 tggtttcttc ccacgtagcg atcggccgct catcaacatg attcatgttt cctgtggcat
     3301 ctcaattctc gtgctgatgg tggttcgtct tttgttaaga ctgaaatacc caaccccgcc
     3361 gattatacct aagccgaaac cgatgatgac gggactggcg catttgggac atttggtgat
     3421 ttatctcctg tttattgcgc tgccagtgat tggtttggtg atgatgtata accggggcaa
     3481 cccgtggttt gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt tcgaacgggt
     3541 tgatagctta aagtcgtggc atgagacgct ggcgaatctg ggatattttg tcatcgggtt
     3601 gcacgctgcg gctgcactgg cacaccacta tttctggaag gacaacacac ttctacgcat
     3661 gatgccgcgt aaacgttcct gaaggatatt taaagaaaac gcctgtacta aaaccgaccc
     3721 gtggtacagg cgaagaatac gggtctacat cggaagcgcc tattatattt atttgtatga
     3781 caaataaaac cataatcctt gcccatacgt ccatctggct tatttttaat caattcaccc
     3841 gatctttgat ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca aatcgcacat
     3901 aagattcgat aaaccatgga ggttatatga aaaaactggc acttattttg tttatgggaa
     3961 cgcttgtttc cttttatgcc gatgccgggc gcaaaccctg ttctggttcg aaagggggga
     4021 tctcacactg tacggcaggc ggcaaatttg tctgtaatga tggttctatt agtgcatcga
     4081 aaaaaacatg cactaactga agtgtaaaag gggtgccatg agaaaatgaa tctgctagtc
     4141 agatgcgcgg ggaaaatccc cgcgcttgcc cttacctgga cgtgcaggcc atgagcgcag
     4201 caacctcctt atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg gtcaggagtg
     4261 tgaatgccag aatcgtaatg cagataatga gcagacacac caccagaggg ttgcgcttca
     4321 tagcctttct ccttgccgga tggcgggtaa gaggctaaca atctgaattg cttggttcat
     4381 tcgttggcct cggtttgtac tgaccccaaa aagttggaca gttaaacacg aggcatatag
     4441 gtctgattcc gatattcatt tggagtcaga ccttttaatt tcaggctaat tcttctgctg
     4501 ttgtagtatt caatatattc cgtaacagca tccttcagtt cgcttatatt act
//
LOCUS       DAJTPG010000194         4544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTPG010000194 DAJTPG010000000
VERSION     DAJTPG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            129..1217
                     /locus_tag="OO230_003947"
     CDS             129..1217
                     /locus_tag="OO230_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5693502.1"
                     /translation="MSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLI
                     FVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSF
                     EATFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRL
                     KMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQ
                     VRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKY
                     LTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRR
                     GDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1217..1951
                     /locus_tag="OO230_003948"
     CDS             1217..1951
                     /locus_tag="OO230_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5693503.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2419..3180
                     /locus_tag="OO230_003949"
     CDS             2419..3180
                     /locus_tag="OO230_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5693504.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3224..4303
                     /locus_tag="OO230_003950"
     CDS             3224..4303
                     /locus_tag="OO230_003950"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5693505.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4300..>4544
                     /locus_tag="OO230_003951"
     CDS             4300..>4544
                     /locus_tag="OO230_003951"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5693506.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1247 a          862 c         1119 g         1316 t
ORIGIN      
        1 atggcatttt ccagcagcga aatgttcttg cgagctggct ggcaacaggt tgcggcgtag
       61 cgttgtatct ggctttaacg gcgcgtactc gttctctgac gttgatatgt ttgttgtcgt
      121 tataccctct gtctacagct ttagtcctta cacaaagccg tgtcggcctt cttggtttat
      181 tttgcgtcat cgtgctggcc gctctcgcgg acagaccccg ctggcgcgga cgtcccctta
      241 gcggtctgtt gcgcttgatc ttcgtcgcct ccttgctgtg ctggtgtgtc ggtatctcaa
      301 actatgccat gccaggggag acaacggctg actttacgca ttcatcatcg aatgaccagc
      361 gtatacgtat tctgaaaggt gccataagtc ttattcaaca gcgcccctta accggttggg
      421 ggttaggcag ctttgaagcg accttcccgc aagccttaga ggatgtgggc gtgacaaaca
      481 tagagagtga tacggctacg catccccata acgaactgct gttcgtaacg gctgaaggtg
      541 gcatcgtcgc cttgtttggt tttttgctgc tatcaggcgt ttggctgtgg ccgtgtatat
      601 gccgcctgaa aatgacaaac aataacgcgc accatcaatg tgaaaagtgg tttattgcct
      661 taatggggtt gcctttagtc attcatatga tgaccgaata tcctctctat acgtctgttc
      721 cccatttgat gctgttgctg ctccttttcc gcatcggaat gccggaaaac gtgatgaatc
      781 aggtacgtat acctaaaaca gtccggttgg gtattctgcc tgtcattgtg ctcattatga
      841 ccgccgcgct ggtcatcctg gtagcgggat ttgatgcaca gcgggaactg accagagctg
      901 aagctgatat gaacgaagga tttatccctc gccttccgga acatagctgg aaatacctga
      961 cgcaggcaga acgtcttgag cgcgatcagc atctgcttat tgcgaataaa ccggggttcg
     1021 ttcgcgatac ggaaagcatg tctcttttca ctgtgtgggg aaataaatgg ttggcaatcc
     1081 acaatgatgc agacgtaagc gctgcaatga tattcattgc gcacaggcgc ggtgattatc
     1141 tgacagctga aaaaataaga aaacgcgcag ctcgggtatt tgtaaatgac gaacgtttta
     1201 ctcgcggggg aaactaatgg ttaacgatct gacgtataag aaattagaaa gcacaaacat
     1261 caactgtaat attctctcgc cagaaaatac cacatggaca aatgtcacgg tgattggcct
     1321 ggatcttttt gccatttgcg gcgtgtgcag tttgctcaag cgtgttgggg gagtgaaact
     1381 tcaggttttc acttccttac ccactgatat gaatgttctg actgacagta gcatcgtgat
     1441 atgggtaaaa atgcgtcacg atggtttgcc tgaattagcc ggacatattg tgaaggtttg
     1501 tcgtcgatac cgtctgatgc ggcaactcgt tattagcgat gctattcctg aaggaatgac
     1561 gaatgatatc gggcctcttt cccgagtatg gcttactcat gggagtgcaa aagtggatat
     1621 tttattatct tcattacgga aggtgttaaa tgctcattgt ttgcctggtc ctttgtttat
     1681 aaaacgcctg ggaagagtgc aatggagggt tcttttactc cgcgcgaagg ggattagcac
     1741 acgtaaaatt gcagaaatct gtggtattgg cctcaagacc gttagcgccc atgagtccgc
     1801 aattcgagaa cgattaggca ccaggaataa aagtgagtac gtctggttag tacgtaatgc
     1861 tcaacttata caacaggcga tacccgcgtt gggaagagat gcgtcaaggt atgaaagatg
     1921 taacaatatg cgaacaaagc aaaaagtata attgatgcgt catagataac tctctttttc
     1981 gatgacgaaa tcgctgagca tgcgtggtga ggttagtaat agatgggtgc tatgttttgc
     2041 cacgggtttg tagctgtgaa acctggaaaa aaataagaat taaaaatatt taaagagagt
     2101 taataaggtt ctggttgtaa ttgtcttggt caaaaaaaag atattgtcaa tgttgttttt
     2161 taaattttca ggttttggtt aaatatttgc ttgcaaatat aggtgatttt tttattgtag
     2221 gcttttgaag gctattataa ctgtgcgcta aattaatgcg ttgaataaaa gaaataacaa
     2281 aattaagaaa agccatattt acgatatggg caacataaat ataatctaaa aaagagtaag
     2341 gcgttgtagg ttggttccgc gttcattacc ttgttagtga acgcggattt atcagttgtc
     2401 gaaatggttt ttataataat gaaaaatttt aatgtactaa ccaaagtcgt attttgcttg
     2461 tcgtttttat caagcgcatt tcccacctat gctgcggatg atgtcaaagt gactcaaaac
     2521 acttcagttt ttaaactgaa gcttagtgca acgcgattaa tctatgaaca agggtcggat
     2581 ggcgcagcga taactgtgac aaatccgcag gattggccga tgcttgtgca gagtaaagtc
     2641 tttgaagaag ataaagtaac tgcagcgccc tttattgtca ctccgccact gttcaggctt
     2701 aatccaggcc agcaaaataa aatgtgggtt gtgcagactg cgccagcttt acaagacagg
     2761 gaaaaattac actggctatg cgccaccggc attacgccta aaaagagtga tgtttgggcc
     2821 gagaaagata aagaaaaaat tgctgaaaat gtgaacctga acatccaggt taacgtacat
     2881 acctgcataa aacttattac caggccatct ggtattgaag gcaagatggt taatgcgagt
     2941 gagaagttat cgtggaaaaa gcagggtgat aaaattgtcg cggtcaatca gtcgccgttc
     3001 tacatcaacc tttcttcttt acaggttggc ggtagcaaga ttgagaaact agattatatt
     3061 gcgccgtttt cgcaacatga gtttgttatg ccaaaaacta gcggccagga tattaaatgg
     3121 tctgtgatta ctgatgaagg tggtgagagt cgtttgttta tatctcattt gcaatcataa
     3181 tgtattacat ttttattgaa tatttttatg ccatgcatat tccatgtttt atcgactgtc
     3241 attaattttg ataatggcct tacttgcggg tcagttaagt gcccaggagt ggtcatttga
     3301 ttcttcacag ttggaaggaa acgttagtgc ggacactgtg gcaatgttta atcagggaga
     3361 gcaactcccc ggaaattatc gtgtggaaat ttatcttaac ggtgaaaaag ttgatgttgg
     3421 ggagtttccc tttcatcgtc cggaatcccc tgaagaaaaa gagcttgttc catgtttaac
     3481 cgttgatgac cttatccact atggcattaa aattgataaa agcagcagtg atactgacaa
     3541 taaaaagaat cagtgtttta aatggaactc tattgaaggt ttgaaagtaa attatgattt
     3601 tgatagccag cgcgtacaga taaccgttcc acaactgtat ctgcaagata aaaaaagtag
     3661 tttagcacca gtgtcgttat ggaatgaggg tgtagctgca ttccgcatgg tgtatcagac
     3721 aaatattgat atttccaaac aaaatgataa tcaatctacc acgcgtaatt ctcgttatgg
     3781 caggttcact ccagggttta atcttggtgc ctggaggttt cgtagttcag tgacatggag
     3841 caaggaactt ggccagtcag aaagatggca aaggggttac atgtggtttg agcgaggcat
     3901 aaatgcgatt aaaagccgat taacgcttgg agaatcttat acgtcgtctg aggtgtttga
     3961 cagcatacct tttcgcggcg ggatgttagc gaccgacgat gcgatgactc cgccggagga
     4021 cagctactac acacctgtgg tgcatggaat agcacaaagt gaggcgcagg ttattattaa
     4081 acaaaatggg caaattattt ttacccgttc tgtgccgcca gggccttttg cgctggataa
     4141 tttaccaacg ctggccgttg gcggggagct tgatgttaca gtgagagaga gtaatggcga
     4201 ggagcagtat ttttccgtgc catttcaaac gccggccatc gctctgcatg aaggatattt
     4261 caaatatagt gtgatggggg ggaatataaa gaagaaggta taaaaagctc ccctgtctcc
     4321 gtgggcgaaa tcacggcgat gtacgggctt ccttatggcg tcacggtgta tggagggatt
     4381 caaaacgcca ctcattttaa tgcaatatct acgggtattg gcatttctct ggggctgtta
     4441 gggagtttat cgacagatat tacgcgttcg attgcgaatt tatattatgg gaataaatat
     4501 cgtattcgtt atagtaaatc cattagtgat tttggtactc aatt
//
LOCUS       DAJTPG010000195         4530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTPG010000195 DAJTPG010000000
VERSION     DAJTPG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..345)
                     /gene="kptA"
                     /locus_tag="OO230_003952"
     CDS             complement(<1..345)
                     /gene="kptA"
                     /locus_tag="OO230_003952"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5693507.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEI"
     gene            595..1278
                     /locus_tag="OO230_003953"
     CDS             595..1278
                     /locus_tag="OO230_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5693508.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1275..1736
                     /locus_tag="OO230_003954"
     CDS             1275..1736
                     /locus_tag="OO230_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5693509.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1749..2921
                     /gene="iadA"
                     /locus_tag="OO230_003955"
     CDS             1749..2921
                     /gene="iadA"
                     /locus_tag="OO230_003955"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5693510.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            2986..3897
                     /gene="hypT"
                     /locus_tag="OO230_003956"
                     /gene_synonym="qseD"
     CDS             2986..3897
                     /gene="hypT"
                     /locus_tag="OO230_003956"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5693511.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(3890..4282)
                     /gene="iraD"
                     /locus_tag="OO230_003957"
     CDS             complement(3890..4282)
                     /gene="iraD"
                     /locus_tag="OO230_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5693512.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4279..4362)
                     /gene="idlP"
                     /locus_tag="OO230_003958"
     CDS             complement(4279..4362)
                     /gene="idlP"
                     /locus_tag="OO230_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5693513.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1068 a         1183 c         1150 g         1129 t
ORIGIN      
        1 tatctcgtca aggaaacggc ttgccgtacc gtggtagaga aactgtggcg gtgttttttc
       61 ggcaaaggaa atagcgacct gcgaagtcga atgcccctga accgcgcgga tgcacctacc
      121 atcactggaa taactaaaac gctttttatc gctggttgcg accacggtat ccagcaatgc
      181 acgggttaat cgcttgcccg ctttttgggc acaaagtatg agcttattga tatccgccca
      241 tccttcacgg tccagtacga taccaatcgc ctccggtttg tgtcggagga caaaacttaa
      301 aaacttgctc gtatccgcca gctctttttc gttgtacttt gccatcgcgt tttcgtccct
      361 ggattttctg tttttattta ctaatgtcat tgtcactggg tttatgaatt ttctgcaact
      421 caatcctgcg cagaaccttt tccacacagc gtaatttttc ccctatgcaa aaaacgcata
      481 gccagaaaca tacttgcatt ttccccatac ccacatggct gataaggttt ttcagttcca
      541 ggaattttcc tggctcaagc aacacacaaa cacaaaaaaa caatggggat tgttatgaca
      601 caacaagggg atgctgtagc cggggagctg gcgacagaaa aagtgggcat taaagggtat
      661 ctggcttttt ttctcaccat aatctttttt cccggtgttt tttccggaac agacagctgg
      721 tggcgcgttt ttgatttttc cgttctgaac ggatcgtttg ggcaactgcc tggtgcgaat
      781 ggagccacca cctcatttcg cggggtgggt ggcgcggggg ctaaagatgg ctttctcttt
      841 gcgctggagc tggcaccgtc agtcattctc tctctgggga ttatttcaat taccgatggt
      901 cttggcggat tacgcgccgc gcagcagtta atgacacctg tgctaaaacc gttgttgggc
      961 attccaggga tctgctcgct ggcactgatc gctaacctgc aaaacacgga tgccgccgcc
     1021 ggtatgacca aagagctggc gcaggaaggt gaaattaccg aacgcgataa agtgattttc
     1081 gccgcatatc aaaccagcgg tagcgccatc atcaccaact acttctcttc cggcgtcgcg
     1141 gtgtttgcct ttctgggcac gtccgtaatc gtacctttag ccgtcatcct ggtgtttaaa
     1201 tttgtcggag ccaacatttt gcgcgtctgg cttaacttcg aagaacgtcg caacccaacg
     1261 caaggagcgc aagcatgaca actcaggtcc gcaaaaacgt catggatatg tttatcgatg
     1321 gcgcgcgccg tggctttact atcgccacga ccaacctgct gccaaacgtg gtgatggcgt
     1381 ttgtcatcat tcaggcgctg aaaattaccg gcctgctcga ctgggtgggg catatctgtg
     1441 aacccgtaat ggcgctgtgg ggattacccg gtgaagcggc aaccgtcctt ttagccgcat
     1501 taatgagtat gggcggcgca gtcggtgttg ccgccagtct ggcaaccgct gacgcattaa
     1561 ccggccacga tgtcaccgtt ttactgcccg caatgtattt aatgggcaac ccggtgcaaa
     1621 atgtcggtcg ctgcctgggg acagccgaag tgaatgccaa atattacccg catatcatta
     1681 cggtctgcgt gattaacgca ttgctgtcga tctgggttat gcagcttatt gtttaacaag
     1741 gagttaccat gattgattat accgcagccg gttttaccct gctgcaggga gcgcatttgt
     1801 atgcgccgga agatcgggga atttgcgatg tcctcgtcgc taacggcaaa attatcgccg
     1861 ttgccagcaa tatcccttct gacattgtac cgaactgcac ggttgtcgat ctcagcgggc
     1921 agatcctctg cccaggtttt attgatcaac acgtccattt gattggcggt ggcggcgaag
     1981 caggtcccac gacgcgcacg ccggaagtgg cgctaagtcg cctgacggaa gcgggcgtca
     2041 cgtcagtggt tggtctgctg ggcaccgact ctatctctcg ccacccggaa tccctgctcg
     2101 ccaagacccg tgcgctcaat gaagaaggca tcagcgcctg gatgctgacc ggcgcttatc
     2161 atgtcccttc ccgcactatt acgggttccg tggaaaaaga cgtggcgatt atcgatcgtg
     2221 tgattggcgt gaaatgcgcc atctctgatc accgttccgc cgcaccggac gtttatcacc
     2281 tggctaatat ggcggcagaa tcccgcgttg gcggtttgct cggcggtaaa cctggcgtca
     2341 ccgtgttcca catgggcgac agtaaaaagg cgttaaagcc tatttatgac ctgctggaaa
     2401 actgcgatgt gccgatcagc aagctgctgc cgacccacgt taaccgcaac gtaccgttgt
     2461 ttgagcaggc gctggagttc gcgcgcaaag gcggcaccat cgatatcacc agcagcattg
     2521 acgaaccggt cgcccctgcc gaaggtattg cccgcgccgt tcaggcgggt attccgctgg
     2581 cacgcgtcac cctcagctcc gacggcaacg gtagccagcc gttcttcgat gacgaaggga
     2641 atttaaccca tatcggtgtt gcaggttttg aaacgttgct ggaaaccgtg caggtgctgg
     2701 tcaaagacta tgatttcagt atcagcgatg ccctgcgccc gctcaccagt agcgtagccg
     2761 gtttccttaa catgagcggg aaaggcgaaa ttctgccagg caatgacgca gacttactgg
     2821 tcatgacgcc agaattgcgc attgagcagg tatacgctcg cggcaaactg atggtcaaag
     2881 acggcaaagc ctgcgtgaaa ggaacgtttg aaacggctta aacggttgtc agccgcccta
     2941 atggacgtag aatgccccca agggcggctg acagagtaaa acgtaatgga tgactgtggt
     3001 gcggttttgc ataatattga aaccaaatgg ctttatgatt ttctgactct ggaaaaatgc
     3061 cgcaattttt cccaggcggc agtcagtcgc aacgtctcgc aaccggcatt cagccgccgc
     3121 atccgtgcac tggaacaggc gattggcgtt gaattgttta accgccaggt gacgccactg
     3181 caactctcag aacaagggaa aatcttccat tcgcagatcc gccatctgtt gcaacagtta
     3241 gaaagcaacc ttgcagagct gcgtggcggc agcgattacg cgcaacgtaa aatcaagata
     3301 gccgctgcac actctctttc cctcggtctg ttaccgtcca ttatcagcca gatgccgccg
     3361 ctctttacct gggcaattga agccattgat gtcgatgaag cggtcgataa actgcgtgaa
     3421 gggcaaagtg actgtatttt ttcctttcac gacgaagatt tgctggaagc gccgtttgac
     3481 cacattcgct tatttgaatc tcaattgttc cctgtctgcg ccagtgacga acacggagaa
     3541 gcacttttta acctcgcgca gccacacttt ccgttactga attacagccg caactcctac
     3601 atggtgcgat tgattaatcg caccctgacg cgccacagtg agttaagttt cagcaccttt
     3661 tttgtctctt cgatgagcga gcttttaaag caggttgccc tcgacggctg tgggattgcc
     3721 tggctgccgg agtatgccat ccaacaagaa attcgcagcg ggcagctcgt tgtgctgaat
     3781 cgggacgaac aggtgatccc gattcaggcc tacgcatacc ggatgaatac ccgcatgaat
     3841 ccagttgccg aacgcttctg gcgtgaactg cgcgagctgg agattgtgct tagctgacat
     3901 tctcaagcgt cgcactgcgc caaccgccat tatcccagac cagatcaaac gttagcggtt
     3961 catcgtcgtg gtacagcatt atctcaaggc tgatcgccag agtgtggcag tacgcttctt
     4021 tcaaattaac ctgcaacgcc gcaatccgtg gctcatgaaa taacagtgca gaacgcagct
     4081 gacggcaata ccagctctgg agatgtttgt cgctcgggtt atgcacttgc cactcgggca
     4141 tgcctgaaac aagtaatgac ggtaatgccg aatgaggaga attaaaaaga gtgaggagat
     4201 cgctgcatac cgatttacgc agcaatgacg ttttattgcc tgaaatttca ggtaaaacag
     4261 cctgaagtga ttggcgcatc attttgcgca ctcctgacgt ttagcaacat atgcggcttg
     4321 ccctgaacac cgggcaccac tgtattgatg ctcattttcc atagccattc ccttttgttc
     4381 attacatcca tgtcagtctg agacaggcct gaatcctgtc agcaaagtat aaaaccttac
     4441 ttgcctatga atatctactg acttttcagg aaaaaagtca tgatttgata aaaatcatca
     4501 aactcaaaaa aaaaacgcta gtatattctg
//
LOCUS       DAJTPG010000196         4315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTPG010000196 DAJTPG010000000
VERSION     DAJTPG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1761)
                     /locus_tag="OO230_003959"
     CDS             complement(<1..1761)
                     /locus_tag="OO230_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5693514.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKR"
     gene            complement(1846..2181)
                     /locus_tag="OO230_003960"
     CDS             complement(1846..2181)
                     /locus_tag="OO230_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5693515.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2181..2537)
                     /locus_tag="OO230_003961"
     CDS             complement(2181..2537)
                     /locus_tag="OO230_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5693516.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2537..4033)
                     /locus_tag="OO230_003962"
     CDS             complement(2537..4033)
                     /locus_tag="OO230_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5693517.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4030..4191)
                     /locus_tag="OO230_003963"
     CDS             complement(4030..4191)
                     /locus_tag="OO230_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5693518.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
BASE COUNT          982 a         1310 c          962 g         1061 t
ORIGIN      
        1 gcgtttatcg ggagataaat acccatcacc acgtggcatc tgccatccgg tcatgtcata
       61 gacaaaatca tggaactgtt tgccccactc gtcgaactta tcattcagac caaatccact
      121 gttcagtgca tcagccacaa aatttttaac aagccaggga tgttcttttt caaactcctg
      181 cgccatcatg cccagttcaa gcaatccacc aatcagactg atctttccga ggccctttaa
      241 tttcagccct gtttttcctg ccgccgttgt ccattgattc tgggcgactg ttgccgcacg
      301 aatacttgcc accgccttca caccaatata cagggcagaa accgttgcta ttgtttttac
      361 tgcctgttcc catcccccca ttgctctgac aacctgatca acctcctgcc agactttttt
      421 cacaaccgga ccgaccgttt cccagttatc aattatgaga tatgcaccac cgacgagaag
      481 cgcaattaat ccctttgccg gcgtcatatt catcacgcta cccatgattt ttgttatgcg
      541 tgtcaatgtg ccaatcgcca cgcccatcgt cagtaatgcc gcaccagttt tcgcgatggt
      601 ttttacgacc tcaggatttt ctctgacaaa cgtgcgcact tcttccagaa aaggcttcat
      661 ctcttttatg ccttcattga gtgaaggcag gaaggtttcc cccagcgtgg aagaaatcgc
      721 attgatctgg ttctgcagca gcaacaactg attttctgtc gtcgcggcac gggcggcata
      781 ttctttctgc attgagccgc catactgctg cgcatccgcc acacgattaa agttggttcg
      841 caacaaatcc agattcgtga gcagcggcgc aatggccccc agggattcct tcccgaacag
      901 cgctttcagc acagcagcct gtttttcttt cggcactttt gccagagaat ccagtacgtg
      961 cagcatggcc ccgcgggcgt ctttctgcat atccgccgcc agttttttcg ggctaatgcg
     1021 caacgagcgg agcacttttt tctgcgattt ggtggcagaa tccctcgctg tcagcgacaa
     1081 cataaaattc tttatccccg tcgcagcaat ttctgactcc acccccattc cggcaatggt
     1141 tgcccccatt gcggcaatct ctccggaggc cacacccgca acactgccta aagggccaat
     1201 acgggtcaca acatcagaaa tctttttcgc acttgcagga ccggtattgc caagatagtt
     1261 aatcttatcc gcaagccctg ccacctctcc ctgtgtcagt ttaaaggcag tgcgccactg
     1321 cgccatcatc tgaccggatt cttctgccgt ggtgtcaaag gccacgccca tcttcacggc
     1381 atcgtcagta aactgcatca gctcgtcacg ggcgatacca gcctgaccac ccgccgccac
     1441 aatttcggcg ataccttccg ccgacatggg cagttctgtt gacaggtcgc gcacctgctc
     1501 cgtcattgcc ttaaacgctt ccggcgtatc cagaccatcc accactttcc ggacatccgc
     1561 catttttgat tcaagggcaa tggcagattt gaccgggagc gccagcgccc ccagtactgc
     1621 ggttccggca cccgctgcgc ccagtgacag gctggcaaac tcctttttaa aacctttcag
     1681 ctggcgctgc attcccttca gcggtgccga taccttatcc acggcagtga tgatcgcctt
     1741 cagctgaaaa ctgtcagcca tgcttcatct cctcgttaat gcggacggcc tcggcttcca
     1801 gttctgtaaa ctgcgaaaca gccacccgtc gcagctccag tggattcagt ttccagaacc
     1861 aggcaacatt gtagagtcgt ttccggaggt gcttcccgtc tccgactggg taaaaaaacg
     1921 caggatctgc atgctggtct taaaaatatc cagttttgcc atctgcgccg ccgatgaaca
     1981 cgggatcccc gccagcaacg ggatatattt cagtgccacc tggctgtcca gtttaatact
     2041 gccttcaccg gaaataatga aaggaaaacc cagcgcctcg atttcgtcat acgtgggctc
     2101 gcgcaactcc agcacatgca gcatttcgtt atgcgctgtc accggttttt ttaaaactat
     2161 ttctgtaacg ttcattgcca ttactgatac cctccctctt cgccgtggaa ttcaagatct
     2221 gccgtgcctt cttcggcatt atgattagcc tctccgtgca gccatgccgc cgaaagcaca
     2281 tacaccatgc cgtttgccag ctcagcggta atcgtcatct ggtctgaggt ggtaattttg
     2341 tcgaccggaa agtttttggg cactttaaac gtgcccttaa cgtaaggtga ccgccaggtc
     2401 tccttgtaat ccacatcccc tgccatgccg acgatgtcat cccggacatt ggtgttcatc
     2461 ggcacttcaa tgccacctgt cagcgataac tgctgaccat ccactttaaa aaaacaggtt
     2521 ccggcgatct ttgccattat gctgactcct ctgaatactg aagacggaac tggttaacca
     2581 ccgcaaagac acgtaactga ttaacataat ccggcgggaa cagcgtattc agtcggttcg
     2641 ggttatctgc atcacgctca actatcagat actgtttaaa cagatcgtaa ttttccacaa
     2701 taccggcacg ctccatctga cgatatgtcg ccagaagttc ccctttgata acggcaggag
     2761 tgacaatcgc ctgccccgga ccaaaacggg taccatcatt tgccagcttg tgacgtccgt
     2821 acttgcttgt gatgaccgat ttcagtttgc gaagaacata tgcactggta tgcagagttt
     2881 cactgtccag atagctgttg tctgccacgc catacgcatt ctttttgtag gtggttacgg
     2941 aacgctggat ctgcaacgtg ccgccttcca cataagccgt cgccacgccg tgagataaaa
     3001 gggtctgctg ctctgtcatg atgaatcgct tacctttcgg tgccggaagc atccccacca
     3061 gctcccccgt ctgtgtcgga cgggcaggat cattccggat aaataccgct tcacgggcaa
     3121 ggcgactggc aaccagttca tcgacaggcg actgggtttc tttttcgtaa ccggcaagcg
     3181 tgatatgttg ctgattatgc atatctccgg caccaaccag ctctgacagc gttcccagtt
     3241 ttgcggtata gacatgcccg tataactggc gcgcataact ccagcgaccg ctgctgtcat
     3301 tcatttcggt catcatcata ttgatggagg cggcatcgtt gaacggcaga ccgataaagt
     3361 cgaatgcctc atcgcccata gcagcaacag cggcggtaag atcaggcgct ccgctaccgg
     3421 cagcaccgac ttccgtcacc acccgaagcc ctgctggcaa aatctcacca ccaccagaac
     3481 cataataatt cagacagacc ggtaactcat taccatacag ccccttatga cgggcagtca
     3541 gcgtcaccac gcctgcatca gatgacgccg taaacggcag ggtgataacc ccgtttaccg
     3601 cttccttaat cgcggtggca accgcagtgg catcatcgcc attcaccaca ggcacctgta
     3661 cacgggagcg cccgacatac agactcaggg tgccgctttc ctctgcttct ccggcaaccg
     3721 tcaccctgac cgtcgccgcc gcccctctgg cttccggtac tgcaataaca tacagttcac
     3781 cgaaaggatc tgtctgacgg tagacttcca ccatacgcgc cagctggctc cccgccccac
     3841 aaatctggcg ggcataatct gccgatggca tcagcaccag gctgttaacc tcaatggctg
     3901 catcgttgct ggcatgcccg atcagtaatg caggcgcgct ggtcaccgct gtatttgccg
     3961 cagaattgtt catctcggcg tagaacaacg gcaccagcgt attcgacgga acagtattaa
     4021 aacttatcgt catgattttt tagccttatc ctgtttaaca cgtaccacat cacccgccgc
     4081 tatacggcgg aaccagtagc tgctttcttc cacatttcgc ccttcggaag gcaaaaggtc
     4141 tccacgggca gggtcaggaa ccgaccgccc tttcaggggt ttcacaaaca tgagggactc
     4201 ttaattctga gggaaacgca tttccagatg gtgctcaatg tcaccatccg ggccagtacc
     4261 aggatcgatg taatccacat caatactcag cagggataaa tccggcaggg cattc
//
LOCUS       DAJTPG010000197         4307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTPG010000197 DAJTPG010000000
VERSION     DAJTPG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            139..888
                     /locus_tag="OO230_003964"
     CDS             139..888
                     /locus_tag="OO230_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5693519.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            910..1476
                     /gene="yieF"
                     /locus_tag="OO230_003965"
                     /gene_synonym="chrR"
     CDS             910..1476
                     /gene="yieF"
                     /locus_tag="OO230_003965"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5693520.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1530..2867)
                     /gene="adeP"
                     /locus_tag="OO230_003966"
     CDS             complement(1530..2867)
                     /gene="adeP"
                     /locus_tag="OO230_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5693521.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3032..3688
                     /gene="yieH"
                     /locus_tag="OO230_003967"
     CDS             3032..3688
                     /gene="yieH"
                     /locus_tag="OO230_003967"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5693522.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            3755..4228
                     /locus_tag="OO230_003968"
     CDS             3755..4228
                     /locus_tag="OO230_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5693523.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1152 a         1071 c         1059 g         1025 t
ORIGIN      
        1 gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa tcgaactttt
       61 agccgcttta gtctgtccat cattccagta aatgattact cttgtattca taatggacca
      121 ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac ggaaggacat
      181 ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat tccccctcat
      241 cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt catgctgtat
      301 ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt tttcagtgat
      361 aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt ggcgttaaca
      421 accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg gtttcatagc
      481 ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat cagtaaacaa
      541 aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt gctaaaacta
      601 accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat tgccggacgc
      661 ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg
      721 tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt agatggcaaa
      781 cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac cagccggatg
      841 atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata gacaaccaca
      901 ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct gcgcaaaggc
      961 tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag catggaagtc
     1021 aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca gcaggaagaa
     1081 ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga cggtgtggtg
     1141 atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc catcgactgg
     1201 ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca gaccagctca
     1261 atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct ggttttcctc
     1321 gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa caaagttgat
     1381 ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg gcaattgacc
     1441 gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc agtaatcatg
     1501 catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac aatcttcagg
     1561 ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg cccggtacca
     1621 atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat cgaaaagctg
     1681 aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc ctgccagttc
     1741 acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc agctgcagcg
     1801 tagtctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa cagcagacca
     1861 acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga ggactcaata
     1921 taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat actgtcgaca
     1981 tacagcgcct gcttcatgcg cgggaatttc cccttctcat ccgccagacc tgctttatcg
     2041 gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa agagaaaatc
     2101 acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac aactgtcatt
     2161 acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat ccagcccagc
     2221 agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga ggccagaata
     2281 gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag attaccgatg
     2341 ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttcagccc catcatgccg
     2401 ataaacaggc cgataccgct ggtaataccc acacgcagac ttaccggaat gttagcaatc
     2461 atccagtaac gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc ccagaagatt
     2521 gcgcccatac cgatctgcca cggcagcccc attgcctgga caaccacaaa agcgaaaaag
     2581 gcgttaagcc ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa tcccatcata
     2641 atgctaccga aagcagcaat cagacaggtt gttacgaaga cggcactggt gtccatgcca
     2701 gcaacgccca gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag gaaggtggtg
     2761 aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt aaaaacacgt
     2821 tccagcatcc tctgaccaga agtctgggtg gtatgttgat gactcattat ctatttccga
     2881 acaaggaggg aaaatccgtc gctatcgtat accaaaatgc gacaataggc gcgtttgtga
     2941 gagacttttt tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac
     3001 gaaaacgtta aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga
     3061 ctgcgacggt acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt
     3121 tcaggaattt ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa
     3181 actgtacgaa attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga
     3241 agctgaacac gtttaccgtg cagaagtcgc tcggctgttc gattcagcca tcgaaggggc
     3301 tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg gtatctaacg gcccaaataa
     3361 caaaatgcag cattctatgg gcaagctgaa tatgttgcac tacttcccgg ataaactgtt
     3421 cagcggctac gatattcagc gctggaagcc agacccggcg ttaatgttcc atgcggcaaa
     3481 agcgatgaat gtaaatgtag aaaactgcat tctggttgat gactcagttg ccggtgcgca
     3541 atctggtatc gacgcaggta tggaagtgtt ctacttctgc gccgacccgc acaataagcc
     3601 gatcgttcac ccgaaagtca ccacctttac ccatctttcg cagttaccgg aactgtggaa
     3661 agcgcgtggt tgggatatta cggcatagtt cttcacactc ccttcactta ccccgcttaa
     3721 attggcgctc aaaggtaagt aaagggagtt tgatatgtct gtttcacgtc gggtaataca
     3781 tcacggactt tattttgcgg ttttaggacc gttaattggt gttctgtttc ttgtcctcta
     3841 catattcttc gcaaaagaac cgctggttct tttggtaata atacaggtac ttccattgtt
     3901 tctcttattg tcgattacta cgggagctat tcctgcattg ttaaccggcg tgatggtcgc
     3961 ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt tgtctggctg gtggcatagg
     4021 tggcgtcgtt atcaccgaga tctattgtgc agttattgta catattaagg gcatggcttc
     4081 ctcggagttg tttgaaaaca ttctttctgg tgacagtctc gttgtccgca tcattcctgc
     4141 attgctggca ggtgtggtga tgagcagaat cattacccgt ctgcccggat tggatatttc
     4201 atgtcctgaa acagactctt taagttaagc gggatacttt atctttgggc tactcaaaag
     4261 cagacaggat gttactatga ctcaaaatat caggccgtta ccccaat
//
LOCUS       DAJTPG010000198         4211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTPG010000198 DAJTPG010000000
VERSION     DAJTPG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO230_003969"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO230_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5693524.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO230_003970"
     tRNA            complement(324..399)
                     /locus_tag="OO230_003970"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO230_003971"
     tRNA            complement(406..480)
                     /locus_tag="OO230_003971"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO230_003972"
     tRNA            complement(597..681)
                     /locus_tag="OO230_003972"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO230_003973"
     tRNA            complement(690..765)
                     /locus_tag="OO230_003973"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO230_003974"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO230_003974"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5693525.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO230_003975"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO230_003975"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5693526.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO230_003976"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO230_003976"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5693527.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1047 c          937 g         1138 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggcttt c
//
LOCUS       DAJTPG010000199         4199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTPG010000199 DAJTPG010000000
VERSION     DAJTPG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            85..753
                     /locus_tag="OO230_003977"
     CDS             85..753
                     /locus_tag="OO230_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693528.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(996..1691)
                     /locus_tag="OO230_003978"
     CDS             complement(996..1691)
                     /locus_tag="OO230_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5693529.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1684..3111)
                     /locus_tag="OO230_003979"
     CDS             complement(1684..3111)
                     /locus_tag="OO230_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5693530.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3122..3841)
                     /locus_tag="OO230_003980"
     CDS             complement(3122..3841)
                     /locus_tag="OO230_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5693531.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1126 t
ORIGIN      
        1 tttgctgata tttaacccga acattcctga tacagactat taatacaaca ttatctgcga
       61 cagattttat tttctggata tattatgagt gaacaaatca aacaggatat cgatctgatt
      121 gagattttat ttcatctgaa gaaaaagatt cgtgttatcc tttttattat ggctatctgt
      181 atggcaatgg tgctgttgtt tctgtatatc aataaagaca atacaaaagt gatttacagc
      241 cttaaaataa accagacaac gccaggtata cttgttagct gtgatagcaa taataatttt
      301 gcctgtcaga ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcagacc
      361 agcccagata tcaagaacag agaaataagg ctggaatggt caggagataa gagagattta
      421 ccaactgctg aagaggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca
      481 gaataccata atggcaggca agttctcgat gagatacaaa cgccttcagc aattaacagt
      541 gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat
      601 ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc catttgcctg
      661 gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg
      721 gtagatgaat accaacaaaa ctctgggcag taataaacag cattctgata gagagtgcct
      781 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
      841 ctttttcact gtaaatatcc tggcattact aatgcctgga tatttacaga agagcagcat
      901 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
      961 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     1021 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgag
     1081 ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg cgctttctga
     1141 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     1201 agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc
     1261 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctct
     1321 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     1381 tgcaaacgtt cactaatccc caattcctcc agcctcgtgt caccgctaat cacgaccgac
     1441 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     1501 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     1561 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     1621 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgctc
     1681 cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac ggaaactctc
     1741 tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag
     1801 tggtgttttg ccgccattga taaaccagct tgccgcatga gcgccggcca tcatcccgac
     1861 tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc gttgctctgc
     1921 ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca gttttgacag
     1981 cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa
     2041 atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa ttggccctgg
     2101 ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag tattcataca
     2161 agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca cctcagaacg
     2221 cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg
     2281 cggccctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag
     2341 catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca ttcccatcac
     2401 tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat tggtcaccag
     2461 gcataccgaa ccggtctctg ccaccgcgaa attacagccg gtaataccta tttcagcact
     2521 gaggaaatct tcacggattt tttgccggat gaataaggtc atcgcttcag gcgtttccgg
     2581 cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat ctttatgaat
     2641 tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat attcacccag
     2701 atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac caatctcttc
     2761 ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg caacctgtaa
     2821 aatgtagcgg gtagcgtctt ctttggtttt tgcaaaatag acgtgaccgc cgttttgcgt
     2881 cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat gatcacgtat
     2941 ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca ttttttgccg
     3001 atttgcccca atacgctgct gcgcgcttgc caccgctttg cgcatgatcg gatcttcaat
     3061 ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca tctttatatc
     3121 ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc ccttcccgtt
     3181 gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa tactcagggc
     3241 ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt tcggccattt
     3301 tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag gtcaacagct
     3361 ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc agcttacggg
     3421 ccaggctaca agatgggtga tacacagctc tcccttgcaa actggcacct acatcgacta
     3481 cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca accttttcgg
     3541 cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt atggcatagg
     3601 tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg gcgatcagat
     3661 ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc tgaccgcagc
     3721 atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc agcacggagt
     3781 ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa ttgacattca
     3841 caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc caccggggga
     3901 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt
     3961 tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     4021 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     4081 taaaaatggc tacatccctt atacctattc agagctactt attcaggggt tattgagata
     4141 tacactgatg gtgtacaaaa aaatcaccga caataatcac gtatcaagta atagtcgga
//
LOCUS       DAJTPG010000200         4159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTPG010000200 DAJTPG010000000
VERSION     DAJTPG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..335)
                     /gene="flhe"
                     /locus_tag="OO230_003981"
     CDS             complement(<1..335)
                     /gene="flhe"
                     /locus_tag="OO230_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5693532.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGG"
     gene            complement(335..2412)
                     /gene="flhA"
                     /locus_tag="OO230_003982"
                     /pseudo
     CDS             complement(335..2412)
                     /gene="flhA"
                     /locus_tag="OO230_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2405..3552)
                     /gene="flhB"
                     /locus_tag="OO230_003983"
                     /pseudo
     CDS             complement(2405..3552)
                     /gene="flhB"
                     /locus_tag="OO230_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3788..>4159
                     /locus_tag="OO230_003984"
     CDS             3788..>4159
                     /locus_tag="OO230_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5693533.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNP"
BASE COUNT         1037 a         1206 c         1018 g          898 t
ORIGIN      
        1 ccaccgcctg gcacttccca gataaatcgc aacggttctg ctgccgctat accggaaaag
       61 gccacggtgg taccgctctg cccctctaat tcgacacaac gagattgcga acacaagcga
      121 acccgcagtc ctgcaggtgt cgggccgata agctgataac gccacgccac cagcgtcatc
      181 aatcctgaag caggttgtcg cgcagaaaga ggtgcagacg acatcgactc accgcgatga
      241 tttagcgtaa ttccgacact acttgcctgc cacatcccct ccccggcggc ttgcaccagc
      301 agcggaaaca ataatatcgc taataaggtt ctcatcattt accgccaatt gtcgccgtca
      361 tgcggatatg tcggttatca gacagttcca gattcgacag gaccactaac tgcggcaagc
      421 tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg gttcaccaac aatactggcg
      481 gcgcacccag catctcctga cgggatagag cttcctgagt ttgcgccagt aaacgatccg
      541 ccagccctgg ctccagtcct cccccgccct gcagcgcctg tagtaacaaa cgttccagcg
      601 gtgtatcgag gccaataaca tggacttcat ctttgccagg aaaccactgc tgggtaatcg
      661 cccgtcccaa cgccacgcgc acgacggcgc ttaattcatg tggatcgctt tggatgggcg
      721 catgttccgc cagcgtttcg agaatggtgc gcatatcgcg aatcggtact ttttcatcga
      781 ggagattttg cagcacttta tgcagtgtgg tgagcgtgac gacgccagga acgagatctt
      841 ccgtcagctt tggcatctcc tgggcgacgc gatccaacag ctgttgcgcc tcctgacgac
      901 caaacagctc tgcggcatgc tggctaatga ggtggttaag atgcgttgct accaccgtgc
      961 tggcctcaac cactgtgtac ccctgaatct gcgcctgttc ttttagcgca ctttcaatcc
     1021 agatagcatt caggccaaat gccggatcga cggtcgcctc accaggtaac gtcccggcag
     1081 cggttccagg gttaatcgcc agccagcgcc ccggataagc atcaccactg ccaatctcca
     1141 cgcctttcat caaaatgcga tagcgggcag gttgcagatc catattgtcg cgaatgtgca
     1201 ccactggcgg cagaaatccc atctcctggg caaatttctt gcggatactg cgtatacggc
     1261 ccaacaactc accatcctgc tggaaatcga ccatcgggat cagtcgataa cccacttcca
     1321 ttcccagaga atcttccagt tgtacatcgt tccacgtcgc ttcgacaacg gtattattct
     1381 ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg cgtatccacc
     1441 aggccagccc cagcaatccg gcagtgaaca gcaaaaatac caggttcggc attccaggca
     1501 ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg ttactgaaaa
     1561 gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta acgatgcccc
     1621 ccgcggcggt agaaatcacc agcgccggaa tttgtgccac cagaccgtcg ccaatggtca
     1681 atagcgtata actttccgcc gcgtgtccca tgctcatgcc agttgcagca cgccgaccag
     1741 caacccgccg acaacgttaa tgaccatgat gaggatcccg gcaatggcat cgccgcgaac
     1801 aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca cttcggagcg
     1861 gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt caatcgccat
     1921 ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcta tacgcccggc
     1981 acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca cgataccgat
     2041 agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc cagccgccgc
     2101 cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa gtgccagacg
     2161 caacaacata gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg tgcgctgggt
     2221 aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa acaacaggtc
     2281 gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg
     2341 tccggcaagg atctgccatt atgtcgattt caggtttgcg ggcaggcgca gcatcgcggc
     2401 cagattactc atgggtcggt ttctcgttaa taaaatccag ggctgccggc accggaagat
     2461 gagtaggtgt acagggcgct gtccaccagc cagacgccag cgtttcagtt gccagaccca
     2521 ggccagcact tccgccaccg cggcgtacag ttgacccggg atttgttgac caatctccgc
     2581 atgtcgatac agcgctcgcg ccagcggcgg cgcttcaagc gtcgggacgt tattttcagc
     2641 gccaatttca cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca ctttcggtgc
     2701 gctcattttg ttttcgtcat actgcaacgc taccgaatag tgggtcgggt tattgacaat
     2761 gacatccgct ttcggcacat cggccatcat ccgacgccgt gcggcagctc gctgcatctg
     2821 acagatccgt cctttaacat gggggtcgcc ttcgctttgt ttgaactcat cacgaatatc
     2881 ctgccgcgac attcgcagct ttttcaggtg gctgaagatt tggaaaaaga cgtcaaatcc
     2941 caccattgga atgacaccaa gcaccaccag cagtgcgcat agccctacca aatccatcgc
     3001 attacccatg gcggtaatcg gtgactcggc cattaagcgc atcatctgcg gccagtgatg
     3061 ccagagaaaa aaccccgtca cgctgccaac caggatggtt ttcaaaattg ccttaagcaa
     3121 ctccgcgcta gtctgtgccg agaatatccg tttaatgccc ggtagcgggt tgagtttgga
     3181 aaacttcggc tgcaaggatt tgccgctaaa taccagcccc cccagcataa ccggagaaat
     3241 gagcgccacc agcaccacgc cgctaatcag cggtagcagc gccagcatgg cttctctgat
     3301 cagcagaata atttgcccga ggatcagatt cgggtctttg ataatactgt gatcaaaatg
     3361 cagcccagcg gagagcatgc ccgacaatcg acgggccagc gacacaccgc caaaccagat
     3421 aacactaacg cccaccagca aaatcagcag tgaggtcagt tcacgggaac gcgggatttg
     3481 cccctcttcc cgcgcttttt ctagtcggtg aggtgtgggg gcttctgttt tgtcgtcgct
     3541 ctcgtcagac acgtcgccaa tcctggaaag agtattaagc gtaaatgatg ccagagcgca
     3601 aagcgttcaa tggtttaagt aaggggcaaa acaggcggga tttagggcgt ttatgcaaaa
     3661 ataggttcag ttgcagaacg ttttgcgaac cattttccag ttctgatatt tagccattta
     3721 gcctatagaa tcgcactccg cctctctgtt agtttgctaa taattaaatt attacaaagg
     3781 tgatcaaatg gaatggcaac aattgcttaa ctgcaaccgt cggaaagata atctaaaaaa
     3841 aaccaacata gcatttttac atccccttgc tagcggaaga caagagatcg agcgggatta
     3901 cgaccgatta ctttttgctg caccaactcg tcggcttgcg gataaaacac aagttttccc
     3961 gttagatcaa aatgacagca tacggacgag gctgactcat tctcacgaag tagcaaactt
     4021 tgctcgcggg ataggaatga ggctcgcttt cgagatgaag gagcacatct ttggcgaaat
     4081 acccacgggg attgtcgttg aacgcgacgt tcctgctctg ttagcagcga tcggactggc
     4141 acacgatctc ggtaatcca
//
LOCUS       DAJTPG010000201         4148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTPG010000201 DAJTPG010000000
VERSION     DAJTPG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..446
                     /locus_tag="OO230_003985"
     CDS             <1..446
                     /locus_tag="OO230_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5693534.1"
                     /translation="SACAPETGLQQLVATPVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            462..938
                     /locus_tag="OO230_003986"
     CDS             462..938
                     /locus_tag="OO230_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5693535.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1001..1222
                     /locus_tag="OO230_003987"
     CDS             1001..1222
                     /locus_tag="OO230_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5693536.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1385..1759
                     /locus_tag="OO230_003988"
     CDS             1385..1759
                     /locus_tag="OO230_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5693537.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1806..2180
                     /locus_tag="OO230_003989"
     CDS             1806..2180
                     /locus_tag="OO230_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5693538.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            2177..2668
                     /locus_tag="OO230_003990"
     CDS             2177..2668
                     /locus_tag="OO230_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693539.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2680..2877
                     /locus_tag="OO230_003991"
     CDS             2680..2877
                     /locus_tag="OO230_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693540.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2962..3522
                     /locus_tag="OO230_003992"
                     /pseudo
     CDS             2962..3522
                     /locus_tag="OO230_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3594..>4148
                     /locus_tag="OO230_003993"
     CDS             3594..>4148
                     /locus_tag="OO230_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5693541.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHY"
BASE COUNT         1003 a         1133 c         1041 g          971 t
ORIGIN      
        1 cttcggcctg tgcaccggag actggcctgc aacaactggt tgccactccc gtccctgatg
       61 aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg
      121 aaccccgtgt cttcggctgg atggaccgtc tgtgtgaaga ctactgcggt ggtatctgga
      181 atctgtacac cctgaacaac ggcggggcat ttatggcacc cgaaccggat gacgatgatg
      241 acgaaacatg ggtactgttc aatgccatga acggtaaccg cgctgaaatg agcccggaag
      301 ccgccggcat tgctgcctgt ctgatgacgt acagtcatca tgcctgtcgt acggagtgtt
      361 atgccatgac ggtccattat taccggctgc gggattacgc cctgcagcat ccggaatgca
      421 gcgccattat gcgcatcatc gactgaatgg aggcagggac aatgcagcag ctttcctttc
      481 tgcccggaaa catgacgccc ggcgagcgca gtctcattct gcgggccctg caaaccctgg
      541 accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcggca cgggaatggc
      601 tgattctgaa catggcggga ctggagcggg aagagttccg ggtgctgtat ctgaacaacc
      661 agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgt acggaagttc
      721 atccccggga agtgattaaa cgcgccctgt accacaatgc cgctgccgtg gtgctggcgc
      781 acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc
      841 tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca
      901 accaggtttt ctcctttgcc gaacatggtc tgctttaacc cgtcacaacc acatcacacc
      961 tgttttcact tttatcttct gtcttcagag gtatcacatc atgagaatca tcacccgtgg
     1021 tgaagccatg cgtattcacc aacagcatcc tgcatcccgt ctttttccgt tctgtaccgg
     1081 taagtatcgc tggcacggca gtgctgaagc gtatactggt cgtgaagtgc aggatattcc
     1141 cggcgttctt gccgtgtttg cagaacgccg taaggacagt tttggcccgt atgtccggct
     1201 gatgagtgtc accctgaact gatgttccgt cacccgggaa acgagtacga ccagatgacc
     1261 tcagacgccg ctgtggatga ggccattctc atcaatgcat acatcttcac cgaaaccggt
     1321 cagcgcgccg gctgacctgt catcgttaaa ccattcttaa ccacttttca gagaggattt
     1381 tatcgtgtca gacaaactct ccgggataac tcatcccgat gacaatcacg accgcccctg
     1441 gtgggggctt ccctgcacag tgaggccctg ttttggcgct cgtctggtgc aagagggtaa
     1501 ccgcctgtat taccttgccg accgcgccgg tatcagaggc cgcttcagcg acgcagatgc
     1561 ataccatctg gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag
     1621 cggtgaactg aatccccgat atcagcatac cgtcacgctg tatgcaaaag ggctgacctg
     1681 cgaagccgac accctcggct cctgtggtta cgtttatctg gctgtttatc cgacaccagc
     1741 acccgcaacc acctcataac cccgaagctc tcacctttag cactaccagt tacagagaac
     1801 ttaagatgaa aacattatcc gacacacatg tacgggaggt atctcgctgc ccgtctcccg
     1861 tcaccatctg gcagacactg ctcatccgac tgctggacca gcattatggc ctcacactga
     1921 atgacacacc gtttgtcgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac
     1981 tgtgtgatgc agttaatttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg
     2041 gattcagcac ctgtccccgc tctcagttaa taaacagtat tgatatcctc cgggctcgcc
     2101 gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta
     2161 agtatccgga ggcgaaatga tgaaactggc cctgacactg gaagccgaca gcgttaacgt
     2221 acaggcactg aacatagggc gcattgtcgt tgacgtcgat ggtattgagc tcgctgaact
     2281 gattaacatg gtctgcgata acggctactc ccttcgtgtt gttgatgaat ctgaccagac
     2341 ctcagcagaa tgcacgccac catttgctac ccttaccggc atacgctgca gtaccgcaca
     2401 tatcacggaa acggacaacg cctggttgta ctcgctgtca caccagacca gtgacttcgg
     2461 tgaatcagaa tggattcatt ttacaggtag cggatatctg ttacgtaccg atgcgtggtc
     2521 atacccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc gtctggtcgt
     2581 cacactcatc cggcgttatg gcgtcagtct cattcatctg gatgccagcg ctgaatgcct
     2641 gccgggttta cccactttcg actggtaacc aggaacaaca tgaaatcatt aaccacggaa
     2701 acagcgctgg atattctgat tgcgtggctg caggacaata tcgactgcgg atcgggaatt
     2761 atctttgaca acgatgagga taaaacggat tcagcagcac tgttgccctg tatcgaacag
     2821 gcccgggagg atgtccgtac cctgcgtcat ctgcagcttc tgcaccagaa ccggtgaagc
     2881 tcactcacca gacttcattc ctctgacgcc agcctgcaca gggctggcgt tttcatttat
     2941 ctgcaaaaag gaatatcgat tatgtcagat atcacaatct cccgtccgga agtggtcaac
     3001 gggcatacgg acgttatctg ctcaaccagc attcgccaca ttctggctgt acgaaagagt
     3061 acactgttgc aaatcgacac acttatccga caactggctg aaatctcggt gctgacggaa
     3121 agcatcggag gtaaaactgc cccggactgg gccatgaaac aggattttcg ctgcggctgc
     3181 tggctgatgg agaaacccga aacagcaatg aaagccatta cccggaatct tgatcgcgaa
     3241 atctggcgtg acctgatgca acgttccggg atgctgtcat taatggatac gcaggcccgt
     3301 gatacatggt accggtcact ggagtacgat aattttccgg aaatcagcga agcgaacatt
     3361 ctgagcacat ttgaacaact gcaccagaat aaggatgagg tgtttgagcg aggggtgatc
     3421 aacgtcttca gagggctgaa ctggaattac aaaaccaatt gcccctgtaa atttggcagt
     3481 aaaattatcg tcaacaatct ggtgagatgg gaccggtggg gataatgata tggtcacccc
     3541 caccttaaca gcagtacgct gttaaggtaa atccctttaa cggagagacc atcatgcaga
     3601 acatcacact tattggtatc gatctcggaa agcactcctt ccacattcat tgtcaagaca
     3661 agtctggtaa ggctcttctg cgtaaaaaat ttacccgtac taaactgatg cagtttctgg
     3721 cgacatgtcc gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttatggctc
     3781 gctgtatctc tgattttggg catgaaacta agctgatttc tccacagttc gttcgcccgt
     3841 ttgtcaaaag taataagaat gattttgttg atgcagaagc catatgcgaa gcggcttcac
     3901 gaccatcaat gcgttttgtc cagccaagaa ctgagaccca gcaggccatg agggcgttac
     3961 accgggtcag agaatcgctc atcagagata aggtgaaaac aaccaaccag atccacggtt
     4021 tcttgctgga atttggaatc agcctgccga taggtgatgc tgtaataaaa cggttgtctc
     4081 tcgtgttggc tgaacacgaa atccccgaat atctgagccg ccttttggtg aggttgcata
     4141 cccactac
//
LOCUS       DAJTPG010000202         4043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTPG010000202 DAJTPG010000000
VERSION     DAJTPG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(886..1077)
                     /locus_tag="OO230_003994"
     CDS             complement(886..1077)
                     /locus_tag="OO230_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5693542.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1142..1465
                     /locus_tag="OO230_003995"
     CDS             1142..1465
                     /locus_tag="OO230_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5693543.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO230_003996"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO230_003996"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO230_003997"
     CDS             2143..2673
                     /locus_tag="OO230_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5693544.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTHWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO230_003998"
     CDS             2680..2913
                     /locus_tag="OO230_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5693545.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3460..3852)
                     /locus_tag="OO230_003999"
     CDS             complement(3460..3852)
                     /locus_tag="OO230_003999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693546.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          918 c         1208 g          951 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcagc ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 cactggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggg caccgggaac gcgcttcagt cagaccttgc
     3121 tgaaaacaca ggaacgagtt aaaacgcata cgggcgcgat tctggtgtgt cttttgtcgc
     3181 tgtgttttgc ctctattgcc agatcgcgct cattcccctg aacttctgtt ttcccgctca
     3241 ggtccccgtc ttcttcgctt ccgaaagcat gaataaaatc cgtttcagct gtctctggct
     3301 ggcaattcct gcgtgattca catggctgca tagctatgca tgtggttaaa atttaccggg
     3361 tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     3421 cgcgccgaac gcgtttcagc ggtgcgtaca attaagatat tacgttaaat tgtgagggaa
     3481 tggtccccat atactgactt tatcataggg atagggttgg tagtgggtcg attgggtttc
     3541 ggcattgttt ccgttaatgc caattgcatt tttgtatttg atgcaacaga aggcgtgaga
     3601 ttctcctggg cctggaaagg ctaagtgttt agaattgatg gcaaaatatc ttccgtccga
     3661 tttttgaagt agttgttgtt tggctgtctc ccaaagggtg ctgttacaaa cttcttgaat
     3721 ttgtagtttt tgcaggactc ttattactgc gttgtcatgt tccaattctt taggggagtg
     3781 tattattctc tcttttaata agctgtttac cacagggttt agttgttgtc taaagcacgc
     3841 agataatgcc attgctacac aattttgttc ccgaggaagc atgtttggcg gggtatgccg
     3901 tgtttgatgt gatatgtcta ttctcataga aggtctctgg cttatagcat caacaaatac
     3961 ggctataaaa caaaattttc atagagtgat tcaagatctt cttgagatcc ttttttgcgg
     4021 cgcgtaatct gctgcctgca aac
//
LOCUS       DAJTPG010000203         3982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTPG010000203 DAJTPG010000000
VERSION     DAJTPG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            38..643
                     /gene="gstA"
                     /locus_tag="OO230_004000"
     CDS             38..643
                     /gene="gstA"
                     /locus_tag="OO230_004000"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5693547.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(687..1550)
                     /gene="pdxY"
                     /locus_tag="OO230_004001"
     CDS             complement(687..1550)
                     /gene="pdxY"
                     /locus_tag="OO230_004001"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5693548.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1609..2883)
                     /gene="tyrS"
                     /locus_tag="OO230_004002"
     CDS             complement(1609..2883)
                     /gene="tyrS"
                     /locus_tag="OO230_004002"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5693549.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(3012..3668)
                     /gene="pdxH"
                     /locus_tag="OO230_004003"
     CDS             complement(3012..3668)
                     /gene="pdxH"
                     /locus_tag="OO230_004003"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5693550.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3727..>3982)
                     /gene="mliC"
                     /locus_tag="OO230_004004"
     CDS             complement(3727..>3982)
                     /gene="mliC"
                     /locus_tag="OO230_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5693551.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
BASE COUNT          969 a         1057 c          972 g          984 t
ORIGIN      
        1 ctaccatact ttaaaccaca gacaaaagga gttaccgatg aaattgttct acaaaccggg
       61 cgcctgctct ctcgcttccc atatcaccct gcgtgagagc ggaaaggatt ttacgctcgt
      121 cagtgtggat ttaatgaaaa aacgtctcga aaacggtgac gattactttt ccgttaaccc
      181 taaggggcag gtgcctgcat tgctgctgga cgatggtact ttgctgacgg aaggcgttgc
      241 gattatgcag tatcttgccg acagcgtccc cgaccgccag ttgctggcac cggtaaacag
      301 tatttcccgc tataaaacca tcgaatggct gaattacatc gccaccgagc tgcataaagg
      361 tttcacacct ctgtttcgcc cagatacacc ggaagagtac aaaccgacag ttcgcgcgca
      421 gctggataag aagctgcaat atgtgaacga ggcactgaag gatgagcact ggatctgcgg
      481 gcaaagattt acaattgctg atgcctatct gtttacggtt ctgcgctggg catacgcggt
      541 gaaactgaat ctggaagggt tagagcacat tgcggcattt atgcaacgta tggctgaacg
      601 tccggaagta caagacgcgc tgtcagcgga aggcttaaag taatagccat cggcccacag
      661 gtcgttgtgg gccgaaatga gatatttcag agctttgttg cgctgaagta atgttctggt
      721 ttggcaatac gatcctgagc agccaccact tgcagctcat attcctgcat tgctttggtg
      781 gtcaccatga tttcgtagac tgcagcggtc acatgttcca gggcctcctg cagcgttgcc
      841 ccctgaagca gtttcaccag cagtaaaccg ctcgtcacat caccaacacc taccggctgg
      901 cgcataccaa aatccaccag tggacggctg atatgccaga cttcatcggc ggtgaccagc
      961 agcatttcaa aacggtcacg gctgtagcca gctcgcgcca ggtgtttaac caacacaatt
     1021 tgtggccctt gctcaatgag ttcgcgcgct gccagaaccg cttcttcgac gttatttacc
     1081 gcatgctcac agagtatttc cagctcaacc agattcggcg caatgatatc gctggcaggc
     1141 aaaccgtgcc gcacatgaaa ctctgcgaca cccggtgcaa cgatacagcc tttttccgga
     1201 tgacccatta ccggatcgca aaaatatttc gcctgcggat tcgctgcttt cacctgacgg
     1261 acgataccga ggatatgttc accctgctcc gccgatccca gatagccact taatacggca
     1321 tcacaggtgt gtaatttatc aatggcggca atgccttgca caatttcggt taaatggctg
     1381 ggcggcatca cgcagccagt ccatttgccg tattgggtgt gattagaaaa ttgaacggtg
     1441 ttcagcggcc agacgttcgc gcccaggcgg cgcatcggaa actctgccgc actgttaccc
     1501 gcatgaccat aaacaacgtg agactggata gcgagaatat ttttcatcat gttccctgta
     1561 taaaaaccag ggggagtgat ttctcactcc ccctttccac ttaatgcatt atttccagca
     1621 aatcagacag taattctttt tgccgcgacg cagtaaggta aaacgaccaa acagacgatc
     1681 ttcttcttta aagaagtatt caggatcgga ctgtttttca ccgttaatgg tgatggcatt
     1741 ggaggcgata gttttacgcg cctgaccacg ggaaggttgc agttcagaat cgaccagcgc
     1801 ctgcatcagg tctgcgccct tttccatctc aaccatcggt acgccgtcct gcgccagctg
     1861 ttcgaagtcc gcttcactca gcgcactcaa agaaccgctg aacaggcatt cggtaatacg
     1921 ttttgccgcc tgtaaacctt cttcaccgtg aaccagacga gtcacctgct ccgccagtac
     1981 atactgggcg cgcggtgctt taccgctgtt tttatcttct tcttctaggg cgttgatctc
     2041 ttcaatgctc ataaaggtga agaacttcag gaagcggtaa acgtcggcat ccgcagtgtt
     2101 gatccagaac tggtagaatt tgtacgggct ggttttcttc ggatccaacc agactgcgcc
     2161 gccttcagtt ttaccaaatt tggtgccgtc tgctttggtg atcagcggaa cggtcaggcc
     2221 aaacacctga ttctgatgca gacgacgggt caggtcgata ccagaagtga tgttacccca
     2281 ctggtcagaa ccaccaattt gcagcaccac atcgtactgt ttgttcagac aggcgaagtc
     2341 ataaccctgc aacaggttgt aggaaaactc agtgaacgaa atcccctgat cttcacggtt
     2401 gagacgctgc ttaaccgctt ctttgttgat catctggtta acggagaagt gtttgccaat
     2461 atcgcgcagg aaggtcagca cattcatatt gccgaaccag tcatagttgt tcgccgcgat
     2521 agcagagttt tctccacagt cgaaatcgag gaacggggca acctgcttac ggattttgtc
     2581 cacccactcc tgaacagttt cttcggtgtt cagcttacgc tcggcagctt tgaagctcgg
     2641 gtcgccaatc agacccgtcg cgccgcctac cagcgcaacc ggcttgtggc ccgcctgctg
     2701 gaagcgtttc aggcataaca atggaacaag atgccccaaa tgcaagctgt cagcggtagg
     2761 atcgaagccg caatagagcg cgatcgggcc ttgcgccagt cgctctgcta acgcttcctc
     2821 gtccgtcacc tgggctacca gcccccgctc ttgcaattgt ttaatcaagt tactgcttgc
     2881 catcaaaatc tccatgtata taacgactgc acctttgccg gtacacgact tttcgccaga
     2941 tgcgaaagag acatagaata aagtgccaga atcaggagta ccagcgatta aagcaagatt
     3001 tttgcatctt ttcagggtgc aagacgatca atcttccacg catcattttc acgctggtac
     3061 aaaaagcggt catgcaggcg atgctcaccg ccctgccaga actcaatctg ttcaaggctg
     3121 acgcgaaaac cgccccaaaa gctcggcaat ggcacttcgc cctgttgaaa cttctgcttc
     3181 agctccagga atttactttc aaggataccg cgggcagaaa tgcgactgga ctgcttcgaa
     3241 acccatgcac caatctggct atcacgcggg cggctatgaa aatatttcat cacttcgaga
     3301 gtcgaaagtc gttctgcttt accgatcacc atcacctggc gttcaagggt atgccacggg
     3361 aacagcaggc taacgcgcgg attattttca atttgatgtg ctttacggct accgaggttg
     3421 gtgtaaaaca ccatgccttt ttcgtcgtaa tgtttgagta aaacgatgcg ctgataaggc
     3481 tgaccatgtt catccacggt agcgaccacc atcgcggtag ggtccgccag tttggcttca
     3541 caagcctgag agagccaacg ttcaaaaagg gttaatggat cggcgggaag atcgcggcgg
     3601 cgtaacccgc ctttggtgta ttcacggcgc agatgcgcga tttgctgcaa ttcgtcgtta
     3661 tcagacatgg ttttctttac ggattgtcag tgggtgacgc tattgtgcgc cgcccctgga
     3721 aaaatctcaa cgctgtggat tttgtaactg acagttattc aagacgatgc ggtcgcgttt
     3781 atagacagtc gcttcctcac ctttcgacca gaaaacatag atcccgtcgg agtaacgcgc
     3841 gccagaggct gaaaggccct gtttgagatg cagtagttga ttatcgtaaa caaaactgac
     3901 ttcctggcgc ggattattca gtttgaccgt caacggtttt tcatcacact ggtattccag
     3961 cgtatcggtt tgcattcgct ca
//
LOCUS       DAJTPG010000204         3869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTPG010000204 DAJTPG010000000
VERSION     DAJTPG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(626..1573)
                     /gene="icsP"
                     /locus_tag="OO230_004005"
     CDS             complement(626..1573)
                     /gene="icsP"
                     /locus_tag="OO230_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5693552.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2954..3106)
                     /locus_tag="OO230_004006"
                     /pseudo
     CDS             complement(2954..3106)
                     /locus_tag="OO230_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3162..3855
                     /gene="ospZ"
                     /locus_tag="OO230_004007"
                     /pseudo
     CDS             3162..3855
                     /gene="ospZ"
                     /locus_tag="OO230_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
BASE COUNT         1201 a          734 c          712 g         1222 t
ORIGIN      
        1 ttatcgctcg ccacgggaat atctgcggca gcgggccaat gataacaggt gtctggaaat
       61 ataggggcaa agccacccac gaacgatcca gcatataatc aagaaattag atttcattta
      121 acataagata gattatgtac accaagaggc gtttccttaa tttctgaatt tacaggctct
      181 tttgctctct ttgctatgct gtggatttgg aaagatagag tgctggctga ttatttaggt
      241 attttcatca gcatgtccct gatagcactg ttccatcagc gctttcaggc gataatagta
      301 gaccttgggt agcctctgcc catgtgtaac ttgatacccc gagaacagcc acgatgatct
      361 aagcggttct tatttctccg gtctcagggt cggtaatgcc aacagtaagt ccacagtaat
      421 caacgaagag ttttttgaac cgccccggaa atcctggaga ctaaacgccc tgagaaagag
      481 gtaaacagaa tgactaaaaa tacacgtttt tcccccgaag tccgtcaacg ggcagttcgt
      541 atggttctgg aaagtcaggg cgaatatgac tcacaatggg cggcaatttt tagccatgaa
      601 aacgtaatca actcggtgaa ccaattcaaa aaatatactt tatacctgcg gaagtgagaa
      661 aaccaatata ttcaatactt gcactatttt taatggtgcc agatatattg gtactttgct
      721 cataaagaga tgtatcacct tttttattag aaatgtaatt tctggagcct tctatataaa
      781 attttgcact gggggtgaca tagtatccaa tattcgcagc aacagagcag taattttggt
      841 tttttatttc atctttaaaa attgtctcag tctgatagtg tttatctgta tcagatgaaa
      901 gaacccaacc actatatttc agttctgctc caaactcaaa attctcatgg cggtaattag
      961 cagttaatcc aatataaggt attttgaaga gctgtttgta acctattgtt ctttcaccac
     1021 taggatgtgc ccctttttta ttcctgctcc ccccgttttc actataaata taactccctc
     1081 ccattgcatt aaaactgtaa cgactctctt ggtaacctgc tattagtcca agtcgataat
     1141 ccaaattatt taataaccaa cctttaagat tcagctcgaa ttcgttagca tcacggagat
     1201 gcgtattggg atgccagctc tggtcggtcc atacctgagg ggtattggaa ttgttccagt
     1261 ctttatcaac catgcttgct ttctggttac ctaaagtagt ccaaccggaa actccgaaag
     1321 acacttttgg aattagcttc caatcgatgc cacctctaac aatagtcgca ttactgtatt
     1381 tccagtccag ttgactaatt tttctccccc cttccttggg atgataaacg cgttcttttg
     1441 ttttgccact cagagatcct agactaatat ccgtactgat gttgtccggt atgaaaagtg
     1501 gatagttagt ggtagcatgt gtagtaaaga tcgcaggtac acaaagtgca agtacaaaga
     1561 attttaattt catcgagaac tctacttttt tggttgaaat gtccatgatc ttcttacttt
     1621 ataagataaa cgtcaatgcc aagcattatt ttaattaaat atttgtttat gttaccaaga
     1681 acatatcttt tattataata tttattgaac ttttgtaaaa ataaaaaaat acctcattgt
     1741 gcgaataaag taacgggggc catagatttc tctacctacc ttttggtgtt catacctgag
     1801 gctgatagtt tcaggattcc gcgacaattc tactcacctt cttgttctct cgccaatatg
     1861 aaatcaatca gaaaagcgtt ccataagaac aacgataaga caataaattg tcatcacggc
     1921 tgcaatagcc aacgtgcctt tcagtaccaa catgacaacc tcctgtattt gacgtaacgt
     1981 gtgctgataa atatgtattt gtatctcaac tgactcaata caacacttaa gtatagtttt
     2041 tcagattttg tttttcccgt gaaatattat ggaaatggca gaagcaatac attgaattcc
     2101 gattcgagtg aatacggcta gcgggcacct cactttagca ctgaagccat caacttttat
     2161 ttgacttatt tcaatggaaa gctttctcac tatcagtaca ggtaattccc atccttttaa
     2221 agtcgagagt cagactgcga ctaatacagt taaagtgacc aaactcagtt atcctctaca
     2281 cattttatct catgtttact tggacaattc attggtgctt ttataaaaga ataaatattc
     2341 tactaattta atgcatcacc taaaatagta ttttttataa tagaactgta tctatagtat
     2401 attattaaag cttaattatt tattatgctt taatgtggtt gtccgattaa ggactaatcc
     2461 ttgaaaacgc cgtatagggc gagagttcac tctattgcga taacgtaacc gtacaatatt
     2521 gtgcgtttcc caaattgacc actactgaac tattctggga tttaaatgca ataaaatggt
     2581 tggttgaagg tcgtggtgat gatgttagtc cttttgagaa tattccagtg ccagatctta
     2641 cttataataa agaggcttgg cagtttggat aagctattgt ccttattcca ctaatacaaa
     2701 ctttttgtaa agtatattaa atatacttca tttcatactg aaatccccgg aagtattcaa
     2761 tcaaacaatt acaccataaa aggaagaggg agccccaatt aagttatcaa gcatgtttat
     2821 gatacctgca gggtataaat tttctggctg caagcagaca catataaaac gtctttattg
     2881 atcctaccca cataatatgg acgcagtcgt aagtgaggct ctgtgagcct acccacgtaa
     2941 tatggacaca ggcctaagcg aggttctggt tttcaaattg ttccggactg aggccgccac
     3001 accaacggtg ccgtcgccag cgattgtaat tgcactcgat ataattaaac actgttgccc
     3061 gcactatttc ccgatagata aagtgttctc cgtggagaca ttccacttag gaacaattat
     3121 attgttcatg gtaatgttta tatttgagta tagagattaa aatgattagt cccatcaaga
     3181 atattaagaa cgtatttcca ataaatactg caaatactga atatatagtg agaaatattt
     3241 atcctagagt tgaacatggt tactttaatg aatcaccaaa catatatgac aagaagtata
     3301 tctctggtat aaccagaagt atggcacaat taaaaataga ggagtttatt aatgagaaat
     3361 ccagacgact taattatatg aagacaatgt attctccatg tccggaagat tttcagccta
     3421 tttccagagc cgaagccagt acaccggaag gaagctggtt gacagttata tctggaaaac
     3481 gtcctatggg gcaattttct gtggatagtt tgtaccatcc tgacttacat gcattatgtg
     3541 agctcccaga gattagttgt aagatcttct ctaaagaaaa tagtgatttt ttgtatataa
     3601 ttgttgtatt cagaaatgat tcttcccaag gagaactacg ggcaaatcgg tttatagaac
     3661 tatatgatat aaaaagagag attatgcaag tgttaagaga tgagtcgcca gagattaaag
     3721 tctattaaat ctgagattat tattgctcgt gaaatgggag aactttttag ttatgcatca
     3781 gaggaaatag atagctacat aaaacaaatg aatgacaggc tttctcaaat caaggctcga
     3841 atgcctgtta cttaatgtat attaattgt
//
LOCUS       DAJTPG010000205         3841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTPG010000205 DAJTPG010000000
VERSION     DAJTPG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..153
                     /locus_tag="OO230_004008"
     CDS             <1..153
                     /locus_tag="OO230_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5693553.1"
                     /translation="REFWCRGDYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPCPGS
                     PFTDRK"
     gene            complement(271..1215)
                     /gene="hypT"
                     /locus_tag="OO230_004009"
                     /gene_synonym="qseD"
     CDS             complement(271..1215)
                     /gene="hypT"
                     /locus_tag="OO230_004009"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5693554.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1560..2279
                     /locus_tag="OO230_004010"
     CDS             1560..2279
                     /locus_tag="OO230_004010"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5693555.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2345..3643
                     /locus_tag="OO230_004011"
     CDS             2345..3643
                     /locus_tag="OO230_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5693556.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT          953 a          844 c          942 g         1102 t
ORIGIN      
        1 agggagttct ggtgcagggg ggactacgtc gatacagtgg gtaagaacac ggcgaagata
       61 caggattaca taaagcacca gcttgaagag gataaaatgg gagagcagtt atcgattccc
      121 tgtccgggca gcccgtttac ggaccgtaag taacgaagtt ggatgcaaat gtcagatcgt
      181 gtgcgcctgt tagggcgcgg ctggtaagag agccttatag gcgcatttga aaaacctccg
      241 gctatgccgg aggatattta ttgcacgtcg tcaattgcgg cgtgctggct cttccattga
      301 tatctgctgg attaaatcgt ctggcatatg ttcctgtaaa atacgccaga ggttctcggc
      361 ggttctattc agtcgggtgt tcattcggta aatataagcc tgaatgggga ctgtcaattt
      421 agcagcatca aggcatatca gacgtttggt ctgtagttca tcgacgatcg accagatcgg
      481 taaccaggca atgccgtatc cgttgagcgc catattcttt aataactcgc tcatcgaaga
      541 gataaatatc gtgcgcccgg taatacctcc aacctctgcc agatgacgat tgaccaacct
      601 gcccatataa gatgtggcgg tatagttgag caaaggaacc tggggctgag tgatatcgaa
      661 tacagggtgc ccctgtgcgt cggcggcaca taccgggtac aactcagatt caaacagttt
      721 caggcagcaa aaaggcgatt gcataagatc ttcatcgcga aacgaaataa taaagtcact
      781 cttgccttcg cgtaacgtat ttacggcctg gacaacatca atggcttcaa catgatagac
      841 aaattctccg ccataggcgg taagtgaatg caccagtttg ggtaatacgc tcagggatag
      901 tgagtgcgcg gctgcaattt taatgttggg aacgccgagt aaactctggc cgtttaactc
      961 ccccaaattg cattcaagct gctgtaataa gctgcgagtc tgggaatgaa agagcttgcc
     1021 ctcttcggtt aattgtaaag gggtggtcgt gcgatcgaat aaaacaacgc cgatcgctgc
     1081 ctccagcgcc ttaattcgtc ggctgaatgc gggttgcgaa agattacgct cttttgccgc
     1141 ctgagaaaaa tggcgacagg cttccagtgt caggaaatcg tataaccatt ttgtctcgat
     1201 attcttcatt accatggcac ctgtattttc aatccaaaga cataataaaa catcccggca
     1261 ataaaaataa tgttctcaat tagcacaacc tggcctttat cattcgtttt gtgcataacc
     1321 ccttcatttc cggtattgac gttgatatca catttcgcca aaaaaacgaa atttttccgt
     1381 cagataacca gaatacatca tcactatggt gaataagcgg aatgttccat agctgcatca
     1441 tgcgtgtctt gcatcgcctt gtacaacctt gcatttctca tcgcccattt cgactgttaa
     1501 aaaagagagc agcagttatt taccgcgtat tgtcattaac gttttgagag gcaggtattg
     1561 tgaaaggttt aattggtgta ctcggtggaa tggggccggc agctaccgtc gacctgttta
     1621 ataaatttgt aacttttacc gctgcacaat gtgatcagga acatattcct ttaattattt
     1681 cttcgatccc ggatattcct gaccgtaccg atgcattaat gcatcatggg cgttcaccat
     1741 tacccgttat gcgtgattat atgcataagc tggaagatgc cggggcagaa tgtattgtta
     1801 ttccctgtaa taccgcgcat tactggttca aagaattaaa agatgcttgt catattgata
     1861 tattaagtat cgtagaaact acgatcaatg aagttcgcgc ctgtggtaaa acccgaattg
     1921 gcttgttagc caccaatgcc acgctatata tgggtttata ccaaaaagga attgagtcgc
     1981 tgggatttac ctgtgtcagc cccgatgccg acggtcagaa aaaagtgatg gaaagtattt
     2041 attccttgaa agctggcaat atagcgcacg cacaaaagtt gatgaatgag caggctgaaa
     2101 tattattttc ccgcggtgca gaaatccttg ttcttggttg tacagaaatt ccggttattc
     2161 tggcgcaagc cgttaaagag cagccttccc gctatattga ctcaacggcg tcactcgttc
     2221 gtgccggaat aaaatggtac gaaaatcgtg tcggtaaaca ttatctttta acccaataat
     2281 catttaaatc gcagccagaa agttattcgc ttttaactga attatattta taacggagaa
     2341 cattatggtt tggctggaaa ttatcgtagt acttggtgca atattttttg gtattcgcca
     2401 ggggggaatc ggtattggtt tatgtggcgg gcttgggctt gccattctga ctctgggatt
     2461 tggtctgcct atggggtcgc cgccagtcga cgtgatcctg attattatga ccgttgttgt
     2521 ggcggcctca gccttacagg ccgcaggcgg aatggattat ctggtacgcg tcgctggtaa
     2581 ttttatgcgg cgtaacccga aatatatcaa cataatcgcg ccgattatca cctggtctat
     2641 gaccattatg gccggtactg ggtttattgt tttttccacg ctgccggtga ttgctgaagt
     2701 ggcgaaagag tccggtatcc gtccttcccg cacgcttgcc ggttcggttg tcgcttcgca
     2761 ggttgcgatt tccggttcgc cgatcagcgc cgcaatggcg gctatgctca ctattatgga
     2821 ggtaaacggt gtcagtttta ttcaagtgat gtcggtatgt cttcccacct cttttgctgc
     2881 cgcgatggtc gccgctttta ttgcttcccg acagggatgt gagttgcagg atgacgaggt
     2941 ctaccttgag cgcctgcaaa aaggactggt gcaaaaatac gagaataata gcagcatcaa
     3001 gccaggtgcg acgctctccg tcggattgtt cctgctggcg acggtcgcta ttgttattct
     3061 tgctgccttc ccccaactgc gtccgggttt tgatatcggt aaaccgatgg aaacacgcga
     3121 tatcattatt atctgtatgc tctctgccgc ctgcctgatg gtggtgctgt gtaagacggc
     3181 gaccgatgcc attattctca cctccacatt ccgtgcgggg atgagttcgc tggcggtaat
     3241 tctcggcatt gtaaccttag gcaccacttt tattgatgcg catttaactg agattaaaga
     3301 tattgctggt gatattttgc aggcgtatcc gatgttgttg gcgctggttc tttttggcac
     3361 ttgcgcgctg ctctactcgc aaggtgcgac cacgccgtta attatcccgc ttgccgtcgc
     3421 gcttgacgtg cccacctggg caattctggc gtcctatgtg gccgtcaccg gagtatttgt
     3481 actgccaacg tatccaacat cactggctgc catggaattt gatactaccg gcacaactcg
     3541 tgtaggcaaa tatgtgctga atcatccatt tatgctgcct gggttggggg gaattattgc
     3601 tggagtggca ttaggctttg tgattgcgcc aatgattatt tagcactatc aaacatcagt
     3661 atcgatggtt tatacacaat aaagacagat atggcggaat atagtctgtt gtaagcgcca
     3721 acaaagaata ggggaattcc gccatgtctg aacatcttca caatatggta ttaagtgttg
     3781 tcgtttaatg atttatttct tggttatgtt gtgtattagc tgcatctatc gctttttgat
     3841 a
//
LOCUS       DAJTPG010000206         3823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTPG010000206 DAJTPG010000000
VERSION     DAJTPG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..222
                     /locus_tag="OO230_004012"
     CDS             <1..222
                     /locus_tag="OO230_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5693557.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            268..477
                     /gene="hha"
                     /locus_tag="OO230_004013"
     CDS             268..477
                     /gene="hha"
                     /locus_tag="OO230_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5693558.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            516..1106
                     /locus_tag="OO230_004014"
     CDS             516..1106
                     /locus_tag="OO230_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5693559.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1346..1606
                     /gene="repA"
                     /locus_tag="OO230_004015"
     CDS             1346..1606
                     /gene="repA"
                     /locus_tag="OO230_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5693560.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1830..1904
                     /gene="tap"
                     /locus_tag="OO230_004016"
     CDS             1830..1904
                     /gene="tap"
                     /locus_tag="OO230_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5693561.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1897..2754
                     /gene="repA"
                     /locus_tag="OO230_004017"
     CDS             1897..2754
                     /gene="repA"
                     /locus_tag="OO230_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5693562.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3455..3646
                     /locus_tag="OO230_004018"
     CDS             3455..3646
                     /locus_tag="OO230_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693563.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1046 a          929 c          955 g          893 t
ORIGIN      
        1 tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggaatgaat
       61 attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta caacacagat
      121 cccgtgctac ctgtccttca gaacgaagcc agacaacaaa aacgtgggct ctggtcagat
      181 gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg caatcctatc
      241 attcatctgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg gctgtatcag
      301 ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa cagagacagt
      361 ttatcaaaca gtgaacgtga atcatttaat tcagcagcag accaccggct agcggaatta
      421 atcaccggaa agttatatga caggatacca aaagagatat ggaaatatgt aaggtaataa
      481 ttcagcaata attaattgcc aggagcagat aaataatgaa gttgattatt tttattctca
      541 ttgttctgat tattgcagcg ctacttatcc ggattatcct cagaagtgta aatcagcatt
      601 ctcctttgct gatgcaattg catgcggcag gtatacggac tggtgatgca gagcgaatac
      661 tgtccagcgg tgaatactgg caacgtcaga agacgctgct gacagaaagg gaagtcagtt
      721 ttatgaaagg actgttcaga attgtggata tgaagcggtg gtatctgtgt ccgcaggtac
      781 gggtagcgga tatcgtccag ctgaacggga atatccggcc acgatcacgc cagtggtggc
      841 agttattcag gatggtgtct cagtggcatg ttgatgtggt tatcgttgag ctgcgttcgt
      901 tcagtattgt tgcagcagta gagctggatg atgccagcca tttacgaccg gaacgcagac
      961 gccgggatat tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc
     1021 acgatgccag aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc
     1081 agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa
     1141 acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt
     1201 cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc
     1261 ttcgccacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc
     1321 cctgtttgag tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt
     1381 gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc
     1441 cgctgttgca agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct
     1501 gaaagatctc atggttgagt actgcgagag agaggggata acacaggctc agttcgttga
     1561 gaaaatcatc aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt
     1621 ggcgtagcat gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg
     1681 tggcagggaa ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa
     1741 tcttctttaa ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa
     1801 caatcaagct atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt
     1861 gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat
     1921 taccgccagg taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt
     1981 ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc
     2041 attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt
     2101 cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac
     2161 cgcgtccagt gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct
     2221 gccggaaaac tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga
     2281 ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc
     2341 acgttcacat ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg
     2401 cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg
     2461 ggtatggatg aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac
     2521 cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg
     2581 cagcgtcagg atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg
     2641 cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc
     2701 atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtta
     2761 cctcctcaga ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc
     2821 acaaaaaatc agcgctacat acaaaaaaca acctcatcat ccaccttcag gtgcatccgg
     2881 tcccccctgt ttttgataca aaacacgcct cacagacggg gaattttgct tatccacatt
     2941 aaactgcaag ggacttcacc ataaggttac aaccgttcac gtcataaagc gccagacgtc
     3001 aaccctacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta
     3061 attacattca tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acaaatatgc
     3121 acctcccacc gcaagcggcg ggcccccaac cgaagccggt ttagttacaa catgcaaaca
     3181 taagccccta aaaaacccaa gtccaacgca aaactgaaac cacaacgccc ccctcataac
     3241 tgaaaagcgc cgccgccccc gcccaaaggg cgggaacaga gtcgctttta attatgaatg
     3301 ttgtaactac atcatcatcg ctgtcagtct tctggctgga cgtatcgaat acacgctcgt
     3361 aagcggccct cacggcccgc taacgcggag atacgccccg acttcgggta aaaccctcgt
     3421 cgggaccact ccgaccgcgc acagaagctc tatcatggct gaaaacgggt atggcttggc
     3481 agggctgggg atgggtaagg tgaaatctgt caatcagtac cggctgacgc cgggcttcgg
     3541 cggttttact ccggtatcac atgtaaccac agcgtgccgc cttccatgcc gctggcgcgg
     3601 catcagaata atacaagcag catttaatgc atttgcaaaa gtgtgagtaa tattaagtgg
     3661 tcgtttcagg gatatcgtta agacttgagc gataggaatt aaaaccccaa aaagattaaa
     3721 aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga acggaaccga
     3781 cgattaaact ggatggctct gattgattca gggtatgaat ggc
//
LOCUS       DAJTPG010000207         3723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTPG010000207 DAJTPG010000000
VERSION     DAJTPG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(75..263)
                     /locus_tag="OO230_004019"
     CDS             complement(75..263)
                     /locus_tag="OO230_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5693564.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            523..858
                     /gene="iraM"
                     /locus_tag="OO230_004020"
     CDS             523..858
                     /gene="iraM"
                     /locus_tag="OO230_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5693565.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1139..1247)
                     /locus_tag="OO230_004021"
                     /pseudo
     CDS             complement(1139..1247)
                     /locus_tag="OO230_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1770..1846)
                     /locus_tag="OO230_004022"
     tRNA            complement(1770..1846)
                     /locus_tag="OO230_004022"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1810..1812),aa:Arg,seq:tct)
     gene            complement(1948..2023)
                     /locus_tag="OO230_004023"
     tRNA            complement(1948..2023)
                     /locus_tag="OO230_004023"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1988..1990),aa:Ile,seq:cat)
     gene            complement(2168..2989)
                     /locus_tag="OO230_004024"
     CDS             complement(2168..2989)
                     /locus_tag="OO230_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5693566.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3004..3360)
                     /locus_tag="OO230_004025"
     CDS             complement(3004..3360)
                     /locus_tag="OO230_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5693567.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3373..>3723)
                     /locus_tag="OO230_004026"
     CDS             complement(3373..>3723)
                     /locus_tag="OO230_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5693568.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1057 a         1000 c          716 g          950 t
ORIGIN      
        1 cgtcagtgca tttagtccca ctgccagact gtcttttatg gcctggaatg ccgggccagt
       61 acgatgacgt agtatcactc cggctcagtt gcaccactga ccaccacatc accttctgct
      121 gcaatcgcct gcatcagggt ataaggggtt atggccaccg gactaccaaa cggctgccag
      181 ccctctttca gtttatgtgt cagcttttcc gcaagatctg acggcggcgc cgccctgaca
      241 acatcatagt gtttaaatgc catggttctt tccaacatct gaaaaataat tctttaaaat
      301 acctgacatg taatacagaa aaaacacaaa accatacctt aaataaaaac ctgattatca
      361 agcagatatg catggataaa ctacaagacg agatataaac caccctgcat ttaaataaac
      421 aataaacaac atcagaaaaa taattctgtt ctatggttta attcaaaaat atcatttata
      481 cttttcagaa catcaccagc aaggcataaa caaggaaact aaatgaagtg gattgtgatt
      541 gatacagtta tccagccatc atgcggaata tctttttcag tcatatggag taaaataaaa
      601 ttaataatct ggtatcaatc ggatgctttc ttacctcctg aaagtatatt tacactgact
      661 cacacaggca tcatgctcaa taacaaagtg ctacctgtaa ccatttacaa cgtagtacca
      721 ttcaataaaa cattctggaa tttaatcaaa aacagccagg aatgccctac aaatacagat
      781 aacgtattga atgaatgctt taataaccgt tgcactctgc aaatatgccc ttatggacta
      841 aaacaacaaa gtccataagg agtttactca catctgacaa aatcaatata aacagcccct
      901 ccggagaggg gctggagagt ggcgctatgt gccattccat ggtgccgggt gcctcccggt
      961 gaattcagta ccagcacctg aatccgcgat tatcccatat acctactcgc tgattgcccc
     1021 tccgcacagg gggattcacc atgccagttt cttttaacaa actccccgca aaccagacaa
     1081 cagtcaaccg cctgaattgt gaagtattta aaaatttctc ccgctaactg atacccggct
     1141 aacagtctgg cgttttcttt ttcagcaacg ggaaagcaac aaccaccaca cccgccacca
     1201 gcacaccgtc agccggcact gacattatcc ggctgctgca atgccattaa caaaaacagt
     1261 aagcaatcac tttttaccgt aacaggtgat aatccagata tgtatctacc ccagatgagt
     1321 aatccgaagt tcatccatac cacaggtcct ggctattctg ttgtactcct gaacaagagc
     1381 aaataattct gaattagcaa ccatgaactc atcgcaaacc ctctgtatag catcactatt
     1441 cagaataata acgtctcttc ccgaaagacg atcaggagta cagaacaaaa ctgtcaaacg
     1501 gctgaaggcc tttgctcgtg ctgcattgac tatatcaata cgctgcctaa ggatgaaaca
     1561 ccccgacgcc tcatcaatat tcactctacc cacaccatat gaatgataaa tacttaatgc
     1621 tgaaaaaacc attagaccgt ataacaaata ctcaatcaat acttaacaga acttttattt
     1681 ttgacaaata tataatattt tcaacaatat cctgagccag gtatatttca gtataaggct
     1741 ctgccggaag gaatctggaa gaatgaatat ggcgcgctgt actggattcg aaccagtgac
     1801 cgattgctta gaaggcaatt gctctgtccg gctgagctaa caacgctgaa taccgataat
     1861 ggaccgccat cggggacccg ccccccgcac caacaaccct gttatcgtgt cgtctgctct
     1921 tcctgataag ctaatggcgg tttgtgatgg tggcccttgc tggatttgaa ccagcgacct
     1981 ggcgattatg agtcgctcgc tctcaccact gagctaaagg gccgggagca gaataataac
     2041 ggtccgtaat taatttcgca ataaaaaaac ccgctcagcg gcgggttgta gaaactcttc
     2101 taacgccagg cataaaacgc ccatcattat gacgaattta ccacagattc cggaaaaatc
     2161 aaccttgtta cctagttacc ttttttaact gccgctcagc ccatgcttct tcaatatcaa
     2221 accgggtcac cagcgcatca taaaatttct taactgtttt ttcccatgac gcgcgtgtta
     2281 tctggtttgt cacctcgcat atagcattaa atgcctccgt tgatggtagt ctttcatagc
     2341 cacgaccacc acaacgctgg cagtctctga taacaggcat accacgtttt accgactctt
     2401 cacgatgaat ggcgacacca cgcccacggc aatccttaca ggcggtggaa acctcaccct
     2461 ttccgccaca ctccggacag gcaactttta ccacctccct gacttttttc cattcttccc
     2521 agtaaaacgg atacacacct ttcgtacact ttgcccatac cggcggctta ccatccggat
     2581 actggatctt gtttgtaaaa actatgcttt caataaattt ttccccatag caacaagggc
     2641 actgcttttt actcgctgcg ctgcgggcat aatcctcaaa agcgtacgaa gccataatgc
     2701 gcatcactac cggttttatt tctgccggaa gttttctcaa cgccgccaca cgatcgcacc
     2761 gactgagtgc ataatctgcc agtaattctg ttgcccgcgc cctgtcattc atactgatgc
     2821 ccattttccc caggaacgca gaaaaaccca tctcagcccg attctgtgtc atgccctgcg
     2881 cggccatcac atcagtgata ctcagcgcat cttttgacgt tgaggccgat gcatcggtca
     2941 ggccagggga ttttggggag tagtatttcg gtaaatcttc cagtttcatt ttttgacctg
     3001 ctcttaatgc attatggggt aaatcttcac ccccagacgt ccaccagata ctggctgacc
     3061 acgaacgata ttgatttcat caaactgctc atcgtccatt aacactcccg catgcgtcag
     3121 cgcatccagc ggtgctttca ggatattgtc caggtcgcga cgacgcttat ccggtggctc
     3181 tgcaatcacc tttatcgcca gccttccgga caggcttaat ttcagccgct gctggcgaac
     3241 aataagcgcc acagcccggc gataacgctt tccctcctcc gagataaaat atgtgctgcc
     3301 acggcgtcgc cagtaagtgt tcaccgtcgg cgggtaaggt aaaaccaaat ctatgagcat
     3361 cagtcacctc ttttacccaa gcacgccagt tgcaaaggcg tgatcaagaa aacgaaaaat
     3421 taaatcaacc tgagaaccat gcttttcttc gaacgccaga ggatccgcat gaagctcgtt
     3481 gtgatgctcc cgacacagcg gtagcgtgaa aatatcgtga gatttggtcc ccattccgcc
     3541 ctgaccatga ccaatcaggt gatggggatc gtcggctggc ttaccacaac acgcacacgg
     3601 ctgtgtcttc acccagcgtt tgtatttctc gttaacccag cggcgacgtt taggtcgttt
     3661 catgaaagat tccggagact caggatcaac ggcaatgctg accaccgtct tttcctgttg
     3721 tgg
//
LOCUS       DAJTPG010000208         3715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTPG010000208 DAJTPG010000000
VERSION     DAJTPG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..723
                     /locus_tag="OO230_004027"
     CDS             <1..723
                     /locus_tag="OO230_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5693569.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVART
                     VLRAVRDTVVSVARAVWNWIFF"
     gene            1095..>3715
                     /gene="ag43"
                     /locus_tag="OO230_004028"
     CDS             1095..>3715
                     /gene="ag43"
                     /locus_tag="OO230_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5693570.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIR"
BASE COUNT          852 a          959 c         1120 g          784 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcagcgttt ccgtctgagt ggccatggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacaga gatgcagagt atgaagccct gtaccgtgac
      181 attctgcctg aacttgacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct
      241 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
      361 tctcccgcac aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg
      421 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      481 agtgcgctaa tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag
      541 gacgagctgc gcacggaatc tgtccggggt caggcccgtg aacagtttac cggtgcggtg
      601 gaccggatat ttgacacggc ggagagcgtc tgtgttgcct ctgttgcccg tacggttctg
      661 cgtgccgtcc gtgacacggt ggtctctgtt gcccgcgctg tatggaactg gattttcttc
      721 tgaacagtct gccagggaaa tgcctgataa cattacgtta accaactgaa aacatgacac
      781 aaacccctca tactcctcct gtttctcttg cgtgaccgcc ttgtcgttaa tagaataaaa
      841 ctatcgataa aacagatatc gatcgttgct atcgatcgtt tatagtggtt aatggtctgc
      901 gaaaaggcag ctgaatctct tcatcatgca gaacggaatt gcacacaacg gactgatact
      961 tctctggcgg atgagagagg ggaagcattt atgtccggga aaaaccactt tatctgacca
     1021 tgctgttttg tacctgccgg tatccacttt tgtgggtacc ggctttttta ttcaccctct
     1081 gtaaggaaaa gctgatgaaa cgacatctga acaccagcta caggctggta tggaatcaca
     1141 ttacgggcac cctggtggtg gcttccgaac tggcccgctc acggggaaaa cgcgccggtg
     1201 tggcggttgt gctgtctctt gctgctgtca catcagtccc ggcactggct gctgacacga
     1261 ttgtacaggc gggagaaacc gtgaacggcg gaacactgac aaatcatgac aaccagattg
     1321 ttctcggtac ggccaacgga atgaccatca gtagcggtct ggagtatggg ccggataacg
     1381 aggccaatac cggcgggcaa tggatacaaa atggcggtat cgccaacaac actactgtca
     1441 ccggtggtgg tcttcagaga gtgaatgccg gaggaagcgt ttcagacacg gttatcagtg
     1501 ccggaggcgg acagagcctt caggggcagg cagtgaatac cactctgaac ggcggtgagc
     1561 agtgggtaca tgaaggcggg attgcaacgg gtaccgtcat taatgagaag ggctggcaga
     1621 ccgtcaaatc cggcgcaatg gcaaccgaca cggttgtgaa taccggcgcg gaagggggac
     1681 cagatgcaga aaatggtgat accgggcagt ttgttcgcgg aaatgccgta cgtaccacta
     1741 tcaatgaaaa tggtcgtcag attgtggctg ctgaaggaac agcaaatacc actgtggttt
     1801 atgccggcgg cgaccagacg gtacacgggc atgcgctgga taccacactg aatggcggtt
     1861 accagtatgt gcacaacggc ggtacggcgt ctggcactgt tgtaaacagt gacggctggc
     1921 agattatcaa ggaaggtggt ctggcggatt tcaccaccgt taaccagaaa ggcaaactgc
     1981 aggtgaacgc cggtggtacc gccacacatg tcaccctgaa acagggtgga gcactggtta
     2041 ccagtactgc ggcaacggtt cttggcagca accgtctggg caatttcacg gtggaaaacg
     2101 gtaaggctga cggagttgtt ctggagtccg gcggtcgcct ggatgtactg gaaggccatt
     2161 cagcgcagaa aacacgggtg gatgacggcg gaaccttggc agtgtctgcc ggtggtaagg
     2221 caacaggtgt caccatgaca tccggtggtg ccctgattgc agacagtggt gccactgttg
     2281 aggggaccaa tgccagcggt aagttcagta ttgatggcac atccggtcag gccagcggac
     2341 tgctgctgga aaatggcggc agctttacgg ttaatgccgg aggactggcc agcaacacca
     2401 ctgtcggaca tcgtggaaca ctgacgctgg ctgccggggg aagtctgagt ggcagaacac
     2461 agctcagtaa aggtgccagc atggtactga atggcgatgt ggtcagtacc ggcgatattg
     2521 ttaacgcagg ggagattcgc tttgataatc agacgacacc ggatgccgcg ctgagccgtg
     2581 ctgttgcaaa aggcgactcc ccggtaacgt tccataaact gaccaccagt aacctcaccg
     2641 gtcagggtgg caccatcaat atgcgtgttc gccttgatgg cagcaatacc tctgaccagc
     2701 tggtgattaa tggtggtcag gcaaccggca aaacctggct tgcgtttaca aatgtcggaa
     2761 acagtaacct cggggtggca acctccggac agggtatccg ggttgtggat gcacagaatg
     2821 gcgccaccac agaagaaggt gcgtttgccc tgagtcgccc gcttcaggcc ggcgccttta
     2881 actacaccct gaaccgtgac agcgatgaag actggtacct gcgcagtgaa aatgcttatc
     2941 gtgctgaagt ccccctgtat acatccatgc tgacacaggc aatggactat gaccggattc
     3001 tggcaggctc ccgcagccat cagaccggtg taaacggtga aaataacagc gtccgtctca
     3061 gcattcaggg cggtcatctc ggtcacgata acaacggcgg tattgcccgt ggagccacgc
     3121 cggaaagcag cggcagctat ggcttcgtcc gtctggaggg tgacctgctg agaacagagg
     3181 ttgccggtat gtctctgacg acaggggtgt atggtgccgc aggccattct tccgttgatg
     3241 ttaaggatga tgacggttcc cgcgccggca cggtccggga tgatgccggc agcctgggcg
     3301 gatacctgaa tctgacacac acgtcctccg gcctgtgggc tgacattgtg gcacagggaa
     3361 cccgccacag catgaaagcg tcatcggaca ataacgactt ccgtgcccgg ggctggggct
     3421 ggctgggctc actggaaacc ggtctgccct tcagtatcac tgacaacctg atgctggagc
     3481 cacaactgca ctatacctgg cagggactta ccctggatga cggccaggat aacgccggtt
     3541 atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc
     3601 acaacgatat gagctttggt gaaggcacct catcccgtga cactctgcgc gacagtacaa
     3661 aacaccgtgt gagtgaactg ccggtgaact ggtgggtaca gccttctgtt atccg
//
LOCUS       DAJTPG010000209         3703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTPG010000209 DAJTPG010000000
VERSION     DAJTPG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            19..897
                     /locus_tag="OO230_004029"
     CDS             19..897
                     /locus_tag="OO230_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5693571.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="OO230_004030"
     CDS             894..2285
                     /locus_tag="OO230_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5693572.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="OO230_004031"
     CDS             2282..2539
                     /locus_tag="OO230_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693573.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="OO230_004032"
     CDS             2634..2834
                     /locus_tag="OO230_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5693574.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="OO230_004033"
     CDS             2827..3207
                     /locus_tag="OO230_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5693575.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            3407..3483
                     /locus_tag="OO230_004034"
     tRNA            3407..3483
                     /locus_tag="OO230_004034"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3441..3443,aa:Ile,seq:cat)
     gene            3485..3561
                     /locus_tag="OO230_004035"
     tRNA            3485..3561
                     /locus_tag="OO230_004035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3519..3521,aa:Arg,seq:tct)
     gene            3564..3639
                     /locus_tag="OO230_004036"
     tRNA            3564..3639
                     /locus_tag="OO230_004036"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3597..3599,aa:Thr,seq:tgt)
BASE COUNT          963 a          819 c         1066 g          855 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaacac gctggtggtc
     3361 agatgagttt gcagatgtga tgacatatgg ttattattct gcctccggcc ctttagctca
     3421 gttggtcaga gcgagcgact cataatcgcc aggtcgctgg ttcaagtcca gcaagggcca
     3481 ccaaccacca ctagctcatc cggatagagc atcaaccttc taagttgacg gtgcgaggtt
     3541 cgagtcctcg gtggtgggcc agcgccgact tagctcagca ggcagagcaa ctgacttgta
     3601 atcagtaggt caccagttcg attccggtag tcggcaccat atgcgggcat cgtataatgg
     3661 ctattacctc agccttccaa gctgatgatg cgggttcgat tcc
//
LOCUS       DAJTPG010000210         3529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTPG010000210 DAJTPG010000000
VERSION     DAJTPG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..442
                     /locus_tag="OO230_004037"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO230_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1027..1743
                     /locus_tag="OO230_004038"
     CDS             1027..1743
                     /locus_tag="OO230_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693576.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2295..2699
                     /locus_tag="OO230_004039"
     CDS             2295..2699
                     /locus_tag="OO230_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693577.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2762..3268
                     /locus_tag="OO230_004040"
                     /pseudo
     CDS             2762..3268
                     /locus_tag="OO230_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          962 a          760 c          804 g         1003 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaatactgg attgtgggcc ataaatattc caaaaagata
      481 gcccgctccg tttattaccg gaacgggcga gagcatgaca ttgtctgtta ttataatgag
      541 actgctgatt ctacttattt ttaatttcaa gaaaagcgtt ttctacaaag aattatgaga
      601 tttaatgttt tgtttagaca atattatcat tctgttcaat aatgaaagtc ttcatcaaca
      661 acaagtaaaa acaacgtccc tctcatcgaa ccggacttgc agatttcccg catccggctc
      721 tcttgcaaca catcagacct tcacgttcgg gcggttatgg ggaaggtcca gcaagctgta
      781 tgagccctaa ctgtttgaat accagtagat taccagaaac ttctatcgat tgaagacatt
      841 accaactagc gttactaaat gttgaccaag ttttgttcac ttgtctggtt ttgatagtag
      901 cattctgcag agtattttga tatgataaat atgcattaac aatgatatta ttgttaagtt
      961 cctagcatga aaattaaaag atcaactttt atctcgaata ttttttacat tatcagttag
     1021 tttcttatga atgacaattc attgcttaga aattcttcat tattcatcgc ctatatggga
     1081 tgtgtagggt gggtcagtgc ttattcttat ggctggggaa cctcttttta ctatggattt
     1141 ccatggtggg ttgttggtgc aggtttggat gatgttgcca gaagtttact ctatgctatc
     1201 atagtaatgg gtatactttt taccggttgg gggattggta ttttattttt tcttcttata
     1261 aagaaaagaa gtaaaataca agacttgagt ttttttagac tattttttgc tattacccta
     1321 ctgtttttcc ctgtaatatt cgaactttta attctcaaac aatatttcat acttccatta
     1381 tcactttcat tcattattag ttcattggtt atttcaataa taattcgtat atatggacgg
     1441 atcttttctg tatcatgttt ttcagatatc ccttttgtta gagaacaccg cattaagtta
     1501 attatggctg gttttttggt ttatttttgg tttttttcct ttcttgttgg ctggtataaa
     1561 cctcaattaa aaaaagaata tcagatgctt tgttataata atagttggta ttatgttctt
     1621 gctcgttatg attcacggct cgttttatct agttcattta aagatgacag taacagattc
     1681 ttaatattta atacagaaca gtcgggattt tatgaaatca atgatgttta tgtcagaaag
     1741 taataatcct ctcccacaaa gtgaattaaa tgaatatata atgcgttcga agtatatgta
     1801 ttgagctgtc atactgagaa acaaacctta gggcagcaca attaaagcca gaaacaacta
     1861 tgcaagaaag ttactaaata agtgctcgga acagtaaaac actcaggggg gactcagtcc
     1921 ggattatgta atcagataaa ttgccaaaca tcacaaatca ccagccggac tgagtgatgc
     1981 cgagcattgc accaataccc cgtgatgaac gatgcctgat gcagaaagct ccctataaaa
     2041 caaagaacaa aaactacgac cggcgattca ccgtcatact gatgctgcat cgtggtgaca
     2101 ctgtctgtca ctttcatgtt gactgaacaa ctctgctgcg aagaaggtgt accgccggcg
     2161 ggatttgatt gtccggcgtc tagcttccac ttccctgatg gaactgaaag ttgttatggc
     2221 cggtcagctt gccctgtccg caggacatga ggtgctgtga cggacagcac cagtaacgga
     2281 aagtgacaat gaaaatgaat actgcgctga tagttgcact aatgtgtatg tggtatgcgg
     2341 tacccgctgc tgcaaaagag actttactgg ctatgccacg taattcaaca gaacattgct
     2401 atgcggagat taatgtgcac ggaccatatg gggtgtattt ccgagtggtc ccgcatcccc
     2461 ccgggggaaa gagctgggtc gaatgtaaca gtgactacta ttactctgat aaaccaccgg
     2521 gagtacagat actcggaacc cgtgcagggt gtcgggtata cggaatctgc gggaccacct
     2581 cgacactgca tgtggctgga agaggggttg tgtgtattaa aaatatatgc agtccacgtg
     2641 ggatgataat tcacaggata cggaaacgtc ctgtagtggc tgtaagcgat gagatgtgat
     2701 cttacccagc aatagtagac acgcggctaa gtgagtaaac tctcagtcag aggtgactca
     2761 catgacaaaa acagtatcaa ccagcaaaaa aacacgtaaa cagcattcgc ctgaattttg
     2821 cagtgaagcc ctgaagcttg ctgaacgcat cggtgttgct gccgcagccc gtgaactcag
     2881 cctgtatgaa tctcagctct atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc
     2941 tgagcgtgaa agtgaactgc ccgcctgaag cggcaactgg cggagcaggc agaggaactg
     3001 gctatcctcc aaaaggccgc gacatacttt gcgcagcggc tggtatacgt ggtgtcagcg
     3061 tcggacagga ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg ttgtcctcgc
     3121 ggcggccttt acccggtcaa aacagcgtta cggtgcacca cgcctgacgg atgaactgcg
     3181 tgctcagggt taccacttta acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct
     3241 gagggcaaag gcctcccgga agttcacgta taggaaattg aaaaaccaga cgataccgct
     3301 gtccacgcct tatgccacgt gagcttttct gcttttccct ccactccggt gttttctctc
     3361 tctgcatggt cgattcttat accgcaacag gctgtttttt atacttgcac cgattcccgg
     3421 gcacacttag cctgtgtcag gccggcatat acctcagcag actgatatcc cgcgcgtttt
     3481 tcttcagccc ctccacattc agtcccgcga tggcccggcg gtataccat
//
LOCUS       DAJTPG010000211         3486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTPG010000211 DAJTPG010000000
VERSION     DAJTPG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..1394
                     /gene="tnaA"
                     /locus_tag="OO230_004041"
     CDS             <1..1394
                     /gene="tnaA"
                     /locus_tag="OO230_004041"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5693578.1"
                     /translation="EPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSEDVFIDLLT
                     DSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYI
                     PVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFK
                     GNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVM
                     DSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKD
                     DSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLV
                     DGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
                     LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEP
                     KVLRHFTAKLKEV"
     gene            1485..2732
                     /gene="tnaB"
                     /locus_tag="OO230_004042"
     CDS             1485..2732
                     /gene="tnaB"
                     /locus_tag="OO230_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5693579.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2864..>3486
                     /gene="mdtL"
                     /locus_tag="OO230_004043"
     CDS             2864..>3486
                     /gene="mdtL"
                     /locus_tag="OO230_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5693580.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRV"
BASE COUNT          794 a          849 c          865 g          978 t
ORIGIN      
        1 ctgaaccgtt ccgcattcgt gttattgagc cagtaaaacg tactactcgc gcttatcgtg
       61 aagaagcaat tattaaatcc ggtatgaacc cgttcctgct ggatagcgaa gatgtgttta
      121 tcgatttact gaccgacagc ggcaccgggg cggtaaccca aagtatgcag gcagcgatga
      181 tgcgcggcga cgaagcctac agcggcagcc gcagctacta tgcgttagcc gagtcagtga
      241 aaaatatctt tggttatcaa tatactattc cgactcacca gggccgtggc gcagagcaaa
      301 tctatattcc ggtactgatt aaaaaacgcg agcaggaaaa aggcctggat cgcagcaaaa
      361 tggtggcatt ctctaactat ttctttgata ccacgcaggg ccatagccag attaacggct
      421 gtaccgtgcg taacgtctat atcaaagaag ccttcgatac gggcgtgcgt tacgacttta
      481 aaggcaactt tgacctcgaa ggattagaac gcggtattga agaagttggc ccgaataacg
      541 tgccgtatat cgttgcaacc atcaccagta actctgcagg tggtcagccg gtttcactgg
      601 caaacttaaa agcgatgtac agcatcgcga agaaatacga tattccggtg gtaatggact
      661 ccgcacgctt tgctgaaaac gcctatttca tcaagcagcg tgaagcagaa tacaaagact
      721 ggaccatcga gcagatcacc cgcgaaacct acaaatatgc cgatatgctg gcgatgtccg
      781 ccaagaaaga tgcgatggtg ccgatgggcg gcttgctgtg catgaaagac gacagcttct
      841 ttgatgtgta caccgagtgc agaacccttt gcgtggtgca ggaaggcttc ccgacatatg
      901 gcggcttgga aggcggcgcg atggagcgtc tggcggtagg tctgtatgac ggcatgaatc
      961 tcgactggct ggcttatcgt atcgcgcagg tacagtatct ggtcgatggt ctggaagaga
     1021 ttggcgttgt ctgccagcag gcgggcggtc acgcggcatt cgttgatgcc ggtaaactgc
     1081 tgccgcatat cccggcagac cagttcccgg cacaggcgct ggcgtgcgag ctgtataaag
     1141 tcgccggtat ccgtgcggta gaaattggct ctttcctgtt aggccgcgat ccgaaaaccg
     1201 gtaaacaact gccatgcccg gctgaactgc tgcgtttaac cattccgcgc gcaacatata
     1261 ctcaaacaca tatggacttc attattgaag cctttaaaca tgtaaaagag aacgcggcga
     1321 atattaaagg attaaccttt acctacgaac caaaagtatt gcgtcacttc accgcaaaac
     1381 tgaaagaagt ttaattaata ctacagagtg gctataagga tgttagccac tctcttaccc
     1441 tacatcctca ataacaaaaa tagccttcct ctaaaggtgg catcatgact gatcaagctg
     1501 aaaaaaagca ctctgcattt tggggtgtta tggttatagc aggtacagta attggtggag
     1561 gtatgtttgc tttacctgtt gatcttgccg gtgcctggtt tttctggggt gcctttatcc
     1621 ttatcattgc ctggttttca atgcttcatt ccgggttatt gttattagaa gcaaatttaa
     1681 attatcctgt cggctccagt tttaacacca tcaccaaaga tttaataggt aacacctgga
     1741 acattatcag cggtattacc gttgccttcg ttctctatat cctcacttat gcctatatct
     1801 ctgctaatgg tgcgatcatt agtgaaacga tatcaatgaa tttgggctat cacgctaatc
     1861 cacgtattgt cgggatctgc acagccattt tcgttgccag cgtattgtgg ataagttcgt
     1921 tagccgccag tcgcattacc tcattgttcc tcgggctgaa gattatctcc tttgtgatcg
     1981 tgtttggttc tttcttcttc caggtcgatt actccatcct gcgcgatgcc accagcacca
     2041 ctgcgggaac gtcttacttc ccgtatatct ttatggcttt gccggtgtgt ctggcgtcat
     2101 ttggtttcca cggcaatatt cccagcctga ttatttgcta tggcaaacgc aaagataagt
     2161 taatcaaaag cgtggtattt ggttcgctgc tggcgctggt gatttatctc ttctggctct
     2221 attgcacgat ggggaatatt ccgcgcgaaa gctttaaggc gataatctcc tcaggcggca
     2281 acattgattc gctggtgaaa tcgttcctcg gcaccaaaca gcacggcatt atcgagtttt
     2341 gcctgctggt gttctctaac ttagctgttg ccagttcgtt ctttggtgtc acgctggggt
     2401 tgttcgatta tctggcggac ctgtttaaga ttgataactc ccacggcggg cgtttcaaaa
     2461 ccgtgctgtt aaccttcctg ccacctgcgt tgttgtatct gatcttcccg aacggcttta
     2521 tttacgggat cggcggtgcc gggctgtgcg ccaccatctg ggcggtcatt attcccgcag
     2581 tgcttgcaat caaagctcgc aagaagtttc ccaatcagat gttcacggtc tggggcggca
     2641 atcttattcc ggcgattgtc attctctttg gtataaccgt gattttgtgc tggttcggca
     2701 acgtctttaa cgtgttacct aaatttggct aaatccttca agaagccagc cattcgctgg
     2761 cttcttgcct ctcaggaaat cacttatgtc caaatggcaa ctcgccttat cctccttcac
     2821 cacgtatgct ttgcgtcacc ttactatcag gacgctttag cccatgtccc gctttttgat
     2881 ttgtagtttt gccctggttt tactttatcc cgccgggatt gatatgtacc tcgttggttt
     2941 accgcgcatc gccgccgatc tcaatgccag cgaagcgcag ttgcatattg cgttctccgt
     3001 atatctggcg gggatggcag ctgcgatgct atttgccggt aaagtggccg atcgttcagg
     3061 gagaaagccg gtcgccatac ccggcgcggc gctatttatt attgcctcgg tgttctgttc
     3121 actggctgaa accagcacgt tatttcttgc aggccgattt ctacaggggt tgggcgcagg
     3181 ctgttgttac gtggtggcgt tcgccatttt gcgcgacacg ctggatgatc gacgtcgggc
     3241 taaagtgctg tcattactca acggtattac ctgcatcatt ccggtgttag cgccagtgct
     3301 cggacatctg attatgctta aattcccgtg gcagagtctg ttctgggcga tggcaatgat
     3361 gggcatcgcg gtactgatgt tgtctttgtt tattttaaaa gaaacgcgcc cagcgtcccc
     3421 cgctgcttcg gacaaaccac gagaaaatag cgagtcgctg cttaatcgtt ttttcctcag
     3481 ccgtgt
//
LOCUS       DAJTPG010000212         3417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTPG010000212 DAJTPG010000000
VERSION     DAJTPG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..681
                     /gene="ospC1"
                     /locus_tag="OO230_004044"
     CDS             <1..681
                     /gene="ospC1"
                     /locus_tag="OO230_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5693581.1"
                     /translation="DKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIR
                     KSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAV
                     TASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYDI
                     EYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKYG
                     ATSDNKYI"
     gene            complement(785..935)
                     /locus_tag="OO230_004045"
                     /pseudo
     CDS             complement(785..935)
                     /locus_tag="OO230_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1011..2708
                     /gene="ospD3"
                     /locus_tag="OO230_004046"
     CDS             1011..2708
                     /gene="ospD3"
                     /locus_tag="OO230_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5693582.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            2825..2944
                     /locus_tag="OO230_004047"
                     /pseudo
     CDS             2825..2944
                     /locus_tag="OO230_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
     gene            3111..3383
                     /locus_tag="OO230_004048"
     CDS             3111..3383
                     /locus_tag="OO230_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693583.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT         1140 a          634 c          686 g          957 t
ORIGIN      
        1 gataaatttt cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt
       61 atagatgtac caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt
      121 gactttatta gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt
      181 gcccctgtgg atctggatag aatcataaac tttgtttttc agccagagta ccatatacct
      241 aggatggtaa gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag
      301 gctgttacag catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct
      361 ctccaggctc tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta
      421 tctaattttg agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt
      481 gagtatctac ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag
      541 ggattggttg atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat
      601 aacgcaataa aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca
      661 tctgacaata aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca
      721 caaaatatcg atatattgtt tattgtcagt atggctcaat gtgataatag ttggaaagtt
      781 tgatgggttt cgccccgttg tagcggtagt cgaactggaa ggtcttcagg cactgcttac
      841 agcgatagag cagccccccc tgaactggaa tggccgttcc ggataccccc ctgagtttca
      901 gagtaacggt gacaaaccac atcaatcttt gccatcaatc atccaaaggg caaagagtac
      961 aacaacacta agtctgcgtc acaacccatc aatgaaagga atatatacat atgccatcag
     1021 taaatttaat cccatcaagg aaaatatgtt tgcaaaatat gataaataaa gacaacgtct
     1081 ctgttgagac aatccagtct ctattgcact caaaacaatt gccatatttt tctgacaaga
     1141 ggagtttttt attaaatcta aattgccaag ttaccgatca ctctggaaga cttattgtct
     1201 gtcgacattt agcttcctac tggatagcac agtttaacaa aagtagtggt cacgtggatt
     1261 atcatcactt tgcttttccg gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa
     1321 aggctattaa tgtgcctgct attatttatt ttgttgaaaa cggttcatgg ggagatatta
     1381 ttttttatat tttcaatgaa atgatttttc attccgaaaa aagcagagca ctagaaataa
     1441 gtacatcaaa tcacaatatg gcattaggct tgaagattaa agaaactaaa aatggggggg
     1501 attttgtcat tcagctttat gatcccaacc atacagcaac tcatttacga gcagagttta
     1561 acaaatttaa cttagctaaa ataaaaaaac tgactgtaga taattttctt gatgaaaaac
     1621 atcagaaatg ttatggtctt atatccgacg gtatgtctat atttgtggac agacatactc
     1681 caacaagcat gtcctccata atcagatggc ctgataattt acttcacccc aaagttattt
     1741 atcacgcgat gcgtatggga ttgactgagc taatccaaaa agtaacaaga gtcgtacaac
     1801 tatctgacct ttcagacaat acgttagaat tacttttggc agccaaaaat gacgatggtt
     1861 tgtcaggatt gcttttagct ttacaaaatg ggcattcaga tacaatctta gcatacggag
     1921 aactcttgga aacttctgga cttaaccttg ataaaacggt agaactacta actgcggaag
     1981 gaatgggagg acgaatatcg ggtttatccc aagcacttca aaatgggcat gcagaaacta
     2041 tcaaaacata cggaaggctt ctcaagaaga gagcaataaa tatcgaatac aataagctga
     2101 aaaatttgct gaccgcttat tattatgatg aagtacacag acagataccc ggactaatgt
     2161 ttgctcttca aaatggacat gcagatgcta tacgcgcata cggtgagctc attcttagcc
     2221 cccctctcct caactcagag gatattgtaa atttgctggc ctcaaggaga tatgacaatg
     2281 ttcccggact tctgttagca ttgaataatg gacaggctga tgcaatctta gcttatggtg
     2341 atatcttgaa tgaggcaaaa cttaacttgg ataaaaaagc agagctgtta gaagcgaaag
     2401 attctaatgg tttatctgga ttgtttgtag ccttgcataa tggatgtgta gaaacaatta
     2461 ttgcttatgg gaaaatactt cacactgcag accttactcc acatcaggca tcaaaattac
     2521 tggcagcaga aggcccaaat ggggtatctg gattaattat agcttttcaa aataggaatt
     2581 ttgaggcaat aaaaacttat atggaaataa taaaaaatga aaatattaca cctgaagaaa
     2641 tagcagaaca cttggacaaa aaaaatggaa gtgattttct agaaattatg aagaatataa
     2701 aaagctgaat attatctcgt ggtgatacac tgaatttgag gtaaccggct catttaaacc
     2761 gtctggtctg ttacctccgg ttccacaaaa ataatgtcca tcattttgaa gtggacacta
     2821 tcgtatggaa taccggacct ggatcactga agctttacgc cttcatttcg aagaacattt
     2881 accacgggtt gtggccggac gtcgcctggg cgtaccaaaa tcaactgtct gtggtatgtt
     2941 cgtgtgaacc gcccccggta tcccgctcat gctggcgaac ccagacacgc agtgaaccgc
     3001 cccgggtttc ctggagagtg ttttatctgt gaaccacggc agcctggcgg ctgcaaatcg
     3061 gggtgtggca gaatatgagc tttcagagta accatccaga atgagttccc atgtcacaca
     3121 acctggagca ccagaaagtc cacaccagaa tggtgaaaga ggttctgaaa gccgtcgcca
     3181 gagccaataa tcacccttat aagtccgtat ttgcagactt tatcaccggt catccctcct
     3241 gcacggtctg tttctgggaa accttccaca aaatgtaccc ggattcaccg tacgaatacg
     3301 tcacgttctg ccatacctgc cgtcgttttg atttatacga aacagaagcg gagatgaagg
     3361 cggatgaccc gaagtggtgg tgattttcag ccccgaccgc tgaaacgcct gttcaca
//
LOCUS       DAJTPG010000213         3399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTPG010000213 DAJTPG010000000
VERSION     DAJTPG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(169..372)
                     /locus_tag="OO230_004049"
     CDS             complement(169..372)
                     /locus_tag="OO230_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693584.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLDGV"
     gene            complement(374..529)
                     /locus_tag="OO230_004050"
     CDS             complement(374..529)
                     /locus_tag="OO230_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5693585.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(732..1139)
                     /locus_tag="OO230_004051"
     CDS             complement(732..1139)
                     /locus_tag="OO230_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5693586.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1216..1443
                     /locus_tag="OO230_004052"
     CDS             1216..1443
                     /locus_tag="OO230_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5693587.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1427..1849
                     /locus_tag="OO230_004053"
     CDS             1427..1849
                     /locus_tag="OO230_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693588.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1927..2704
                     /locus_tag="OO230_004054"
                     /pseudo
     CDS             1927..2704
                     /locus_tag="OO230_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2711..3399
                     /locus_tag="OO230_004055"
                     /pseudo
     CDS             2711..3399
                     /locus_tag="OO230_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          866 a          752 c          926 g          855 t
ORIGIN      
        1 atcccccatg gagatggttc gtataatcaa ctcccggacg gaacttatgt ttccgaaaag
       61 ggcggtgata tttatcaaat tcgcagccag atatctgact atgcagaccg actatccggg
      121 tatcgcgcgt ctgtttttgt ttgcgaattc agtcaatgcg catggtattt ataccccgtc
      181 aagacccggt gaatatcctg cacggccttc tgctttgtag aaggcttctt cgctatcata
      241 atcgccagat tccagcgctt ctttagccag ccagatgcgt gccccagtgc ctgcatttgg
      301 ctccagttgc tggaggcgtt ttgcatcttc taggagtaga gcgataacgt gttttaattc
      361 tgtctcgttc attttactca cctgaatgtc ttcccaacca acgacgtgcg ccagcttcgg
      421 ttttaaacgt tttgcttttg gtatacgtta tggcggtgaa tgtgccgtcc tggttgggga
      481 acacgccgta caccggagat tcgttgttgc caagatcgat agtattcatg ttgaccccat
      541 ttccccttaa cgccggggta gcggaactgt ttgctgagaa caccgtgcgg tgtcttgatg
      601 aataaaattt agaataacct aaggagggtg atcaagattt ttgtgtagaa aaacctaagt
      661 tttttgatgt aaaaaacaca agtgtttgaa agtttgtgct ttttattaca gggtgtggag
      721 aaaaaagggg attatttgtt tgcgcttctt ttgcgagctt tgagtagttc ttcaaaaagt
      781 ttgttgaaat tttcaactcg agcacgcatc tctgacaaca gggccttttg ctctgactca
      841 ggcagtgcgt cgaacagttg aagtaactct ttttgatctt ctgtcagaat ggctggctga
      901 ttatccggga tcggttcgcc tggttgttta tcttcatccc caaaaagaag ccaagtcggt
      961 gagcactgaa gcgcttggct cagtgcgaat aatcttttcc ccgccggctg tgtttcatct
     1021 ctttcccatt gagaaattgt tacgtgagcc actttgacca gcttacctaa tgcggcctga
     1081 gacagtttta attttttacg cctatgtaag aggcgagcac cgaaggtttc gtttttcata
     1141 ttagggaatt ctaatttttc ttgacttagg tttctctacg gcctggtttc cttaggaaaa
     1201 tctaaggagc tcgatatgtt gaaaattgat gctatagcgt tttttggcag caaaacaaag
     1261 cttgccaatg ccgcaggagt taggctggca agcattgctg catgggggga actggttcct
     1321 gaaggtcgcg cgatgcgcct gcaagaggca tctggcgggg aacttcagta cgaccccaaa
     1381 gtttatgacg aatatcgtaa ggcaaaacga cctgggaagg tgattcatga aaatcaggca
     1441 tgagcacatc gaatcagtgc tgttagccct ggcagccgaa aaagggcagg cgtgggtcgc
     1501 taacgcaatt actgaagaat atctgcggca ggggggcggc gaattgtctc tggtaccagg
     1561 caaggactgg aacaatcagc agaatatcta tcaccgttgg ttgaaaggtg aaacgaaagc
     1621 gcaaagggaa aaaattcaga aactgatccc tgcggttctg gcaattcttc cgcgtgagct
     1681 gcgtcaccga ctctgtatct tcgataccct ggaacgccgt gcattactgg cggcgcagga
     1741 agcgttgagt acggcaattg atgcgcatga tgatgctgtc caggccgttt accggaaagc
     1801 acatttcagc ggtggtgggt cgcccggcga ttctgtcgta gtgcattgat tgaaattaat
     1861 cgtgccggat tgttttgttc ggtatcagtt aaatgtaacg ctgcgagcgt tacaaggtga
     1921 aaacaaatgg cttcaaactg gataaagctc gaggttatta cgccggataa gccggaaata
     1981 ttcaggcttg ctgagattct gaatattgat ccagatgccg cattagggaa ggttattcgc
     2041 ttctgggcat gggcggatca acaaatgata gacggtaatg cagattgtaa cgctcgcggc
     2101 gttacaaaaa gtgcaataga tcgcatcact tttatgtctg gttttgctga tgcgttaatt
     2161 caggttggat ggctggtcga aaatgacggt gggctttctc tacctaactt tgaacgtcat
     2221 aacggaaaaa gctctaaaaa acgggcggtt acaaacgagc gagtaacaaa aatacgcgaa
     2281 ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc gttaccagtg
     2341 gaagaggaag aggaagatct aaatactgat ctccccctaa atccccctcg ccaaaaacga
     2401 gcgtctaaaa aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc
     2461 tggcatgagt gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc
     2521 gatacgggaa ctggaaaaat tccgtcagca gggttttaca cctgagcagg tgattcgaca
     2581 cagcatcgcc aatgaatacc agggcctgtt cgcgccgaaa ggtgttcggc ctgagacgtt
     2641 gctccgacag gttaacaccg tctcgttgcc ggacagtgcg atcccgccag gcttcagggg
     2701 gtaacggacc atgaaaaata ttgcgacagg cggcgttctg gaacgcatcc gccgactgac
     2761 cccgcaacat gtaaccgccc cattcagaac ggtagcggag tggcgcgagt ggcaacttgc
     2821 tgaaggccag aaacgtagcg aggagatcaa ccgcctgaat cgccagttgc gggtggaaaa
     2881 aattctgaat cgctcaggca tccagccgtt gcaccgtaaa tgctcgtttg cgaattacca
     2941 ggtgcagaac gacggccagc gatacgcgtt aagccaggcg aaatccatcg ccgatgaact
     3001 gatgaccggg tgtacaaatt ttgcgttcag cggaaaacct gataccgggt agaatcactt
     3061 agcggcagct atcgggaatc gcctgctgaa agacggtcag acagtgattg tggttaccgt
     3121 ggctgatgtt atgagcgccc tgcacgccag ctatgacgac gggcagtcag gcgaaaaatt
     3181 tttgcgggag ctgtgcgaag tggatctgct ggttcttgat gaaattggca ttcagcgcga
     3241 gacgaaaaac gagcaggtgg tgctgcacca gattgttgat cgccggacag cgtcgatgcg
     3301 cagcgtgggg atgctgacaa acctgaacta tgaggtgatg aaaacattgc tcggcgagcg
     3361 ggtgatggat cgcatggtca tgaacggcgg gcgctgggt
//
LOCUS       DAJTPG010000214         3368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTPG010000214 DAJTPG010000000
VERSION     DAJTPG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..238
                     /locus_tag="OO230_004056"
     CDS             <1..238
                     /locus_tag="OO230_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5693589.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO230_004057"
     CDS             248..886
                     /locus_tag="OO230_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5693590.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO230_004058"
     CDS             889..2052
                     /locus_tag="OO230_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5693591.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3368
                     /locus_tag="OO230_004059"
     CDS             2136..>3368
                     /locus_tag="OO230_004059"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5693592.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESEL"
BASE COUNT          750 a          794 c          971 g          853 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctg
//
LOCUS       DAJTPG010000215         3323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTPG010000215 DAJTPG010000000
VERSION     DAJTPG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            453..1280
                     /locus_tag="OO230_004060"
     CDS             453..1280
                     /locus_tag="OO230_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5693593.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1324..2073
                     /locus_tag="OO230_004061"
     CDS             1324..2073
                     /locus_tag="OO230_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5693594.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2070..2639
                     /locus_tag="OO230_004062"
     CDS             2070..2639
                     /locus_tag="OO230_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693595.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2763..3005
                     /locus_tag="OO230_004063"
     CDS             2763..3005
                     /locus_tag="OO230_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5693596.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3009..3155
                     /locus_tag="OO230_004064"
     CDS             3009..3155
                     /locus_tag="OO230_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693597.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3164..>3323
                     /gene="xisR"
                     /locus_tag="OO230_004065"
     CDS             3164..>3323
                     /gene="xisR"
                     /locus_tag="OO230_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5693598.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          927 a          795 c          855 g          746 t
ORIGIN      
        1 cgccatcctg acaagcgttt tatcttcaac gggaatgaga tatggaaacg tggagaatca
       61 accacagagt atacccgcgc acaccagcga acaatcagac gactgcgcag actcatcgcc
      121 aggaaacagt atacagaaaa atggttcaga aaatatgaca catggagcgc cggatattac
      181 gaactgatgg caacaaaacc attctgacgt aaacgaaatt aaccatgacg caattaaata
      241 aggcaagccg aatacatcag gaggaccatg aacatttatt tcagaatagt tatatcactg
      301 gcaattatcg catgtattta cggattaatc gttccggccc tcatatcaat gaaggatacg
      361 gtagcagtga tttctggctt tgctctggcg tgtctgaccc cgccctgcat ttatgccatt
      421 tataagggtc tttctttttc taaggataaa agatgaaaaa agttattcct gctttagccc
      481 ttgttctgct gaccaccgga cttgtcggtt gcgatcgcgt tgagcctggt aatgttggca
      541 tcaaggtaaa caaactgggc gacgataaag gcgtcggaga agtggtcggt gttgggcgct
      601 actggacggg atggaacact gaggtttaca tcttcccgac cttcaaacaa atgaagacct
      661 acgatgagcc attcagcttc cagatgagtg acggtacaac catcggctat cacatcggcg
      721 tggcctacaa agttgatcca tccaaagtta ccacagtctt tcagacctac cgcaaaggcg
      781 tggacgacat taccgacacc gacctgcgcc agaagatagc cgacgcactc aaccgactgg
      841 ccagcaaaat gaccaccgac aaatttatcg acggcggcaa gtctgagctg ctggatgcag
      901 ctcttaaaga cattcaggaa gaaatgacgc ccatcggtat tcaggtaatg agcctctcat
      961 atgtgggtaa gccggagtac ccgcctactg ttatcgacag cattaatgcc aaagtcacgg
     1021 cgaaccagaa aaccctgcaa tgcgagcagg aagtaaaaca gcgcgaagcg gaagccaaca
     1081 tgttgcgcgc ggaagctgcc ggacaggcag atgcgattcg cacaaaagcc caggccgaag
     1141 ccgatgctat tcgtttacgc ggtgaagctc tgcgccagaa cccaaacgtt atggagctgg
     1201 aagccatcaa caagtggaac ggtacactgc cgcaatacat gaccagcggt gccaacacac
     1261 catttattca ggttaagtaa tacatatgcc cggtattaca cgccggggtg tctggagata
     1321 aaaatgaata ctgtaaccat caacaacaaa cagtttccgg taatcgaata tcgcggtcag
     1381 cgtgttgtga cgctggcgat gattgatgaa gtccaccagc gaccggaagg aaccgcgcgt
     1441 gcagcattca accgaaaccg tgagcatttc atcagcggtg tggattatgc cgaattaggt
     1501 gcggacgtaa tacgtacgga cctcccggaa ggaacattct ctaaatttgc accatcgggg
     1561 attgtacttt ttgaatcagg ttacctgatg ttgacgaagc cattcaatga cgcccttgcc
     1621 tggcaggttc agcgcgaact ggttaacagc tacttccgca ctcgcacacc gctaacggaa
     1681 atcgagatga tcgctgcgat agccgccgac gctgttcgtc aacagaagcg cctgaatcat
     1741 gtggaagaac agctcgaaac agtcacagaa gcggtggaaa ccatcaaacg cggaaatatg
     1801 cgcgccggat atgtcggtta ccgccaggtg gtagccaaaa gtggaatgag tgacaccaag
     1861 tgtcggaatc tggtgaacgc atatcaaatt cctacagaca cccacgaatt catgactccg
     1921 gacggtctgt tgtctcgcag ggctatcgtc gagcttgagc catttatggc ggcgttccac
     1981 caaatgatgt cagaggctga cccacgcggc acacgctggt atcacccgaa gatggggctt
     2041 ttccaggtga tcggatggga ggataaagca tgatcatcca gtcaaaactt attcgcgccg
     2101 ctctggtgtg cgctgctaaa aacgacgttc gttattacct gaacggtctt cacatcacgc
     2161 cgaaacatat tgaggcaacc aatggttccg tggcactgcg catggctcac ggcatccgga
     2221 cgaagaaaaa catcattgtc cagttcgaag ggggcgtccc ggccaaagcc gaaacgacag
     2281 agctgatttt tagtaaagag ccgatcgctg ttcatcgcga ccagtttcag cgacgactgt
     2341 ccattaccgg cattaaactg gtggacggtt gttttccgga tttggatcgc atcattccga
     2401 aaaagtttga ccgctgtact cacccggtgc tccaggcggg ttacctgagt tatccagaga
     2461 agatgtttgg tcgtgagcgt aaatttattc ccgtccagtt acgtccctcc ggtgacgggc
     2521 aagcggtcag aattcagttt gattccatca tcaactcaat gtatggcaat cctgaatttg
     2581 ttgtgatgcc ttgtcgtgat catggcgatt tcaacgtggc tcaggagcat ccggaatgaa
     2641 aatcgaatac caggacgccg caggaggtga atcaatgagc tggcctgatg caatcgtaac
     2701 tctggggatg gtattcgcag tagcgtttgt tgtgtactcg atttgtcgat ggggataacc
     2761 acatgttcgc tttgattcaa cgcgggcaga tatacacaga cagagccgga tacccagtag
     2821 aaattactcg cagtactgag cactcagtgt tctttcggag gatggacgga cgtaccgggc
     2881 gagtacgcat cggtgaattc agtagcctgt tcgagcatat tgaccacctg gaatatcaca
     2941 agattctggc tgaaacagaa caagaaaagc acctgaagaa attacgcgca atgcaaagga
     3001 agtaaaaaat gaataaagcg tttgagctat gggtccacca gcgttacggc aaccactacg
     3061 acctgacgcg agatgttgac ggtttctact gtcgtgaaat tgtgaaacga atgtttgagg
     3121 tctggtgcca ctgccgtggg ctgagtgttg tgtgaggtaa tgcatgggca atgtgattca
     3181 actggctccc aatgaatggg tttgtgaaag cgttctaatc gcaattaccg ggctcaaacc
     3241 aggcacaatt cttcggaccc ggaaagaatg ctggatggtt ggaagagagt atattcacgt
     3301 atcaccagac ggtaatccaa agc
//
LOCUS       DAJTPG010000216         3239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTPG010000216 DAJTPG010000000
VERSION     DAJTPG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(1..229)
                     /locus_tag="OO230_004066"
                     /pseudo
     CDS             complement(1..229)
                     /locus_tag="OO230_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            473..592
                     /locus_tag="OO230_004067"
                     /pseudo
     CDS             473..592
                     /locus_tag="OO230_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            842..1708
                     /gene="ospB"
                     /locus_tag="OO230_004068"
     CDS             842..1708
                     /gene="ospB"
                     /locus_tag="OO230_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5693599.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            2037..2777
                     /gene="phoN2"
                     /locus_tag="OO230_004069"
     CDS             2037..2777
                     /gene="phoN2"
                     /locus_tag="OO230_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5693600.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(3137..>3239)
                     /locus_tag="OO230_004070"
     CDS             complement(3137..>3239)
                     /locus_tag="OO230_004070"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5693601.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         1015 a          621 c          632 g          971 t
ORIGIN      
        1 tttgggcatg atactgatgt agtcacactt tattgttttc acgaagctct ttgctattct
       61 gttactctcc ggactccgca ccgccgtgtt cttcggttca acaaagtccc aacatccggg
      121 tgaactggcg tgtttcatta gctcggtagc atgaaccatt atccgtcagt cacttcactg
      181 gagacgccgg aagctcgttg ccgaagcggt gttccaccgc tcccagcatg tcgccaacgg
      241 gagagcatct acggtctgat tgaactgttt attcaaattg tccaccgtct gtcagtacgc
      301 tctgaacgtc ggctggttaa aacactgctc gctgattttc agagggttca cggtaaaaca
      361 gcacttttat tccggattgc ggaagcggct ctgaataatc cggatgggct ggttaaagag
      421 gttgtgtacc atctgcatat tgttgaacca gatcgcagcg gtaagagaag ttcatcatac
      481 ttggcgcagt tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg cagaacatta
      541 ccggaacaag atagcggtct acctgcactg gtatcagacc cggggcttcc ccggatgatc
      601 tccacggtac tttaggtact gtgtctatat tatgtaggta ggatcaatta actttgtagt
      661 tgaaattctt tcattgaaat aatgaataaa aaagacaaac cgcattaaac aatgccagaa
      721 tataattgca tttttgttcc gtttttttaa atcatagtat acaaagaagc ttgcatacta
      781 tatgaaaata taacaccctt tactatgatg tttataaaac aatatatgga gtcatgtagg
      841 tatgaattta gatggtgtta gaccatactg tagaatagtc aataaaaaga atgaaagcat
      901 atcagatatt gcatttgcac atataataaa aagggtaaaa aattcatcat gtactcaccc
      961 aaaagcagca ttggtttttt taggagagaa aggtttttgt gatagcaatg atgttctatc
     1021 tattatggga caacaaatac caagagtatt taagaacaag atgttatatg attatgtttt
     1081 taaaaatgaa aaaagtaaaa atgattttct aaaaatggct gaatcatggc taccacagag
     1141 tgaaccaata gtaataaata atgatgatga cgcattgaat gctgctgctt atttttctgt
     1201 aaaaaaagcg aaaataaaaa cagtaaacga tactgatttt aaagagtata ataaggttta
     1261 tattcttggg cacggtagtc ctggttctca tcaattaggc cttggttcgg aacttattga
     1321 tgtacaaaca atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg
     1381 ttttacttca tgcggctccg ctgataaagt ggctcctaaa aattttaaca atgcccctgc
     1441 tgaaagtctt tcttgtatcc ttaactctct gccttttttt aaggaaaaag aatctttgct
     1501 agagcagata aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc taaaaatatc
     1561 cggctatcat ggatatggag ttcactatgg tcaagagctt tttccctact cacattatcg
     1621 ttcaacttca attcctgctg atccggagca tacagtaaaa agaagctctc agaaaaagac
     1681 ttttattatt aataaagaac tggattagta taaaattttt aacctatagt taattgtttg
     1741 catttaagat gaagttattt tatctttagt agaaaagtgc ttccctatga gggaaaatca
     1801 caaatcatca taatacagag acaaaacgat acgaaaaaat agataaaaaa catcattctt
     1861 ttaccacatt attttccgtg aatatgaaaa ataatgttat tactttaata taagactatt
     1921 ttttgttttt ccatcactct gttcaaattt ttccgcatga cttgtgtttt ttgtaataca
     1981 gctcgttttt tacagctgac caaaatcatc aattaattat gctaaggaaa taaattatga
     2041 aaaccaaaaa ctttcttctt ttttgtattg ctacaaatat gatttttatc ccctcagcaa
     2101 atgctctgaa ggcagaaggt tttctcactc aacaaacttc accagacagt ttgtcaatac
     2161 ttccgccgcc tccggcagag aattcagtag tatttcaggc tgacaaagct cattatgaat
     2221 tcggccgctc gctccgggat gctaatcgtg tacgtctcgc tagcgaagat gcatactacg
     2281 agaattttgg tcttgcattt tcagatgctt atggcatgga tatttcaagg gaaaataccc
     2341 caatcttata tcagttgtta acacaagtac tacaggatag ccatgattac gccgtgcgta
     2401 acgccaaaga atattataaa agagttcgtc cattcgttat ttataaagac gcaacctgta
     2461 cacctgataa agatgagaaa atggctatca ctggctctta tccctctggt catgcatcct
     2521 ttggttgggc agtagcactg atacttgcgg agattaatcc tcaacgtaaa gcggaaatac
     2581 ttcgacgtgg atatgagttt ggagaaagtc gggtcatctg cggtgcgcat tggcaaagcg
     2641 atgtagaggc tgggcgttta atgggagcat cggttgttgc agtacttcat aatacacctg
     2701 aatttaccaa aagccttagc gaagccaaaa aagagtttga agaattaaat actcctacca
     2761 atgaactgac cccataaagc tggacagcct gtatcaggct atggagggcc catagacaaa
     2821 tctaccctat atgagcatag gaggagtcta tgggcacacc acgttttacc cctgaattta
     2881 agggattact ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa gcttattatg
     2941 ctgccatcgg aaacgatgat ctggcaacct gagttcacag ataaaacatt ctctaggaaa
     3001 ctcggggcgg ttccgttcac cacatgcaat gtggtgttgc aggggaacgg tctgcaccat
     3061 cccctatgtc gatcaatata acagaaatga caacttcaga ttcagggcac aacctaaata
     3121 tattttaggt cacctctcaa atcgtttgcc tgataccgct ccatttttta ataaaaaaat
     3181 caatcatttt tgaatctttg cttttcattg cattgtcaag catagtgtcc ccactattt
//
LOCUS       DAJTPG010000217         3201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTPG010000217 DAJTPG010000000
VERSION     DAJTPG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1660)
                     /locus_tag="OO230_004071"
     CDS             complement(<1..1660)
                     /locus_tag="OO230_004071"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5693602.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKA"
     gene            1792..2901
                     /gene="apbC"
                     /locus_tag="OO230_004072"
     CDS             1792..2901
                     /gene="apbC"
                     /locus_tag="OO230_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5693603.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          765 a          802 c          838 g          796 t
ORIGIN      
        1 tggctttcac ttcttcttta gaggcttcca ccagagcttc aacctgtttc atatcgatgc
       61 ggttatacag cgccttgaac ggattcactt tgtggcccag cagcggttgc tggataccat
      121 cccaggtcag ttccgtattg aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag
      181 gcttcaggta agtcatcagc acgcggaaca ggttgatgcc cattgagcaa atcgcctgca
      241 ggtcagcatc gcgaccttcc tgttttgcga ctacccacgg agcctgctcg tcgacatagc
      301 ggttagccag gtcagccagt gccatgattt cgcgcactgc tttaccaaat tcacggcttt
      361 cccacgcttc accaatcact tcagcggcat cggtaaaggt tttgtacagc tcagggtcag
      421 ccagctcgct tgccagcaca ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg
      481 ccaggttaac cactttgttc acgatatcgg cattcacacg ctgaacgaaa tcttccaggt
      541 tgagatcgat atcatcaatg cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt
      601 cagcgtcaaa atgattcagc caggtgctgg ctttaataaa ggtgccgcga gacttggaca
      661 tcttcgcgcc gttcaccgtc acatagccat gaacaaacag gttggtcggc ttgcggaagt
      721 tgctgccttc cagcatggca ggccagaaca ggctgtggaa gtaaacgata tctttaccga
      781 tgaagtggta cagctcggcg gtggagtctt tcttccagta ttcatcgaag cttacgctgt
      841 cgccgcgctt gtcgcacaga ttcttgaaag aacccatgta gccaatcggt gcgtccagcc
      901 agacgtagaa atatttgccc ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc
      961 gggagatatc ccactgttgc aggccggatt caaaccactc ctgcattttg tttgccacct
     1021 gctcctgcaa cgcaccgctg cgggtccatg cctgcagcat ttcgctgaaa gagggcagat
     1081 caaagaagaa gtgttcagaa tcacgcatca ctggtgtagc accagaaacc accgatttcg
     1141 gctcgatcaa ttcagtcggg ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt
     1201 gatccgggga tttacatttc gggcaggtgc ctttcacaaa acggtccggc aggaacatgc
     1261 ctttttccgg atcgtacagc tgagagatgg tgcggttttt aataaaaccg ttttctttca
     1321 ggcgagagta gataagttct gacaactggc ggttctcttc gctgtgcgtc gagtgatagt
     1381 tgtcatagct gatgttaaag cctgcgaaat cagtctgatg ctcctgactc atttcgccaa
     1441 tcatctgctc cggggtgata ccgagctgct gagctttcag catgatcggt gtaccgtggg
     1501 catcgtcggc gcagatgaag ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga
     1561 catcagcctg gatgtgctcc agcatatggc cgaggtggat tgagccgtta gcgtacggca
     1621 gtgcgcacgt caccagaatt ttcttcgcga cttgagtcat agtaggcatt acttcttaat
     1681 agtgaaaagg ggttagatag taccaaatgg gaaaatgtta agtaagtact tctctatatt
     1741 cttcaggtaa actatcagct gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa
     1801 caatcccagg ccaaatcacc agaagccttg cgcgcaatgg tcgccgggac actggccaat
     1861 tttcagcacc caaccctgaa gcataacctc accacgctga aagcgttgca ccatgtcgcc
     1921 tggatggacg acacgttgca tgtggaactg gttatgccgt ttgtctggca tagcgcgttc
     1981 gaagagttaa aagagcaatg tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc
     2041 gactggaaac tgtcgcataa catagccact ctgaaacgcg tgaagaatca gccgggaatt
     2101 aacggtgtga agaacattat cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc
     2161 acggcggtaa acctggcgct ggcactggcg gcggaagggg cgaaagtcgg cattctggac
     2221 gccgatatct acggcccatc aattcccacc atgctgggcg cagaaaatca gcgtccaact
     2281 tcgccggacg gtacccacat ggcaccgatt atgtctcatg gcctggcaac caattcaatt
     2341 ggttatctgg tcactgacga caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg
     2401 ttaatgcaga tgttgcagga aaccttgtgg ccggatctcg actatctggt gcttgatatg
     2461 ccgccaggca ccggtgacat tcaattgacg ctggcgcaaa acattccagt aactggtgca
     2521 gtcgtggtca ccaccccgca ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg
     2581 ttcgagaaag tcgaggttcc ggtgctcggt attgtcgaga acatgagtgt gcatatttgc
     2641 agtaactgtg gtcaccatga accgattttc ggtaccggtg gtgcagagaa actggctgaa
     2701 aaataccata cacagctgct ggggcagatg ccgttacaca tctccttacg tgaggatctc
     2761 gataaaggca cgccaacggt tatcagccgt ccggagagcg aatttaccgc tatctaccgc
     2821 cagctggctg accgcgttgc agctcagctc tactggcagg gtgaagtcat tcctggcgag
     2881 atttccttcc gcgcggtcta acgcctatta accattctgg ttattcttca tacaccgcag
     2941 agatgttata ttgatgcggt gtatttgttt ttgccgcatt aatgaacact tatttaatac
     3001 tgcgaatatt ctgcggacct tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa
     3061 tgcttcatta tcatccttcg tgctgtgtag tggatgtaca gctatagttt gttatgcact
     3121 cattctatcc aggcataacc tttactacag ggataacgtt caatgaataa gtattggctg
     3181 tcaggaatta tttttcttgc c
//
LOCUS       DAJTPG010000218         3178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTPG010000218 DAJTPG010000000
VERSION     DAJTPG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO230_004073"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO230_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5693604.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO230_004074"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO230_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          773 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggaccct gagcaggtgg taattgtaag acgttgcggg
     2821 cgacggtatt acaattgcac atcctgtcct gtttctttag gacgtatcat ggcacagcat
     2881 atcaactgat aaataatccc tgtgcagtgc agccgctgtt gcataaaatt tcgctctgcg
     2941 tcgagtgttt cttaatcaat aattccgctg atactccgga aaaacgcgag tctgttctca
     3001 tgcctgaaat gcccacaccc cacgcaaaaa caagtttttg ctgatttttc tttataaata
     3061 gagagttatg acaaattagt tcttcttgct ctctttgtga tatttaaaaa agcggtgtcg
     3121 gcgcggtgtt gtagctgcgc caacaccgct ttttaggggt ggtactgact attttcat
//
LOCUS       DAJTPG010000219         3099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTPG010000219 DAJTPG010000000
VERSION     DAJTPG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(106..1815)
                     /locus_tag="OO230_004075"
     CDS             complement(106..1815)
                     /locus_tag="OO230_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5693605.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO230_004076"
     CDS             complement(2247..2966)
                     /locus_tag="OO230_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5693606.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1039 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgc
//
LOCUS       DAJTPG010000220         3093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTPG010000220 DAJTPG010000000
VERSION     DAJTPG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            2..2905
                     /locus_tag="OO230_004077"
     rRNA            2..2905
                     /locus_tag="OO230_004077"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3001..>3093
                     /gene="rrf"
                     /locus_tag="OO230_004078"
     rRNA            3001..>3093
                     /gene="rrf"
                     /locus_tag="OO230_004078"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          815 a          683 c          966 g          629 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     3061 cgtagcgccg atggtagtgt ggggtctccc cat
//
LOCUS       DAJTPG010000221         3064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTPG010000221 DAJTPG010000000
VERSION     DAJTPG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..785
                     /locus_tag="OO230_004079"
     CDS             <1..785
                     /locus_tag="OO230_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5693607.1"
                     /translation="KKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAP
                     IIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDM
                     QRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPT
                     VRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEM
                     VRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1134..2306
                     /locus_tag="OO230_004080"
     CDS             1134..2306
                     /locus_tag="OO230_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5693608.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2360..2983)
                     /locus_tag="OO230_004081"
     CDS             complement(2360..2983)
                     /locus_tag="OO230_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5693609.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          954 a          643 c          606 g          861 t
ORIGIN      
        1 gaaaaaaagg gaaacctgtt ccagaataca cgccaaaacc agcgtccgtt gcaacgaagg
       61 ctacgcatct ttcatttata aaggccctac taagagccgc agagcgtgaa tggaaaatgc
      121 tggataaggc accaattatt aaagtgcctc aaccaaagaa taaacggatc cgctggctgg
      181 agccccatga agcacaaagg ctgattgatg aatgtccgga gccattaaag tctgttgttg
      241 aatttgcact ggcaacaggc ttaagacgct cgaacatcat caaccttgaa tggcaacaaa
      301 tagatatgca gcgccgggtg gcatggataa acccggaaga gagtaaatca aaccgcgcaa
      361 ttggcgttgc gctgaatgat actgcatgtc gcgtattgaa aaaacaaatc gggaatcatc
      421 accgttgggt atttgtgtac aaggaaagct gtaccaaacc agacggaacg aaagcgccaa
      481 cggtcaggaa gatgcggtat gacgcaaaca cagcctggaa agcggcgctg agacgagcag
      541 gtattgatga tttcagattt cacgacttga gacacacctg ggcaagttgg ctggttcaag
      601 ccggagtccc gttgtcagtg ttacaggaaa tgggaggctg ggagtctatc gaaatggttc
      661 gtcgatatgc tcaccttgca cctaatcacc ttaccgaaca cgcacggcaa atagactcga
      721 tcctgaaccc atcggtccca aattcgtccc agtcaaaaaa taaggaaggt actaatgatg
      781 tgtaacttat tgatttaaat ggtgccgata ataggagtcg aacctacgac cttcgcatta
      841 cgaatctgta gcaccaatca taactatctg ttttagcaag cattaaccgc attcactaag
      901 ctatagttga tgacacaaac agaaagttga tgcatgatgt tgccatatat atgtcacaaa
      961 tacggcacaa cgatcttcaa acatatagcc acacattcac agaagagcac aaagccttgc
     1021 aatccagtgc aaagcttggt gtgtctccgt tttgtttcat cagttacaca taactatatg
     1081 atcattaact aaaagtcagt atacttggag aaattttaat tgcgtaatag agcatgcata
     1141 tgattgaaat taacagttta ttattaataa catccgtgat attgatgtcg ttattagctg
     1201 tggggttatt tgataaaatt tccccaataa atcttgttga gcacggcagg aataatcaaa
     1261 tagatggaat gcgggggttc ttagcaattt tcgtgcttat tcatcacgca gcaatttgga
     1321 atggatactt gtcatctgga gtatgggaag caccttcatc aaatctgtta gcaaatttag
     1381 gccaagttgg tgtgtcattc ttttttatga ttactggtta tctgttcttt tcaaagatta
     1441 tctctggaga tcaggactgg acaaggcttt atgtatcaag attactacga ttaaccccaa
     1501 tgttcatagt tagcctatgt ctcattttta tcattgtagg ttttaagtct ggatggagaa
     1561 tgcaggtatc cacagaagag ctttttgtgt caataatgaa gtggttgcca ttcactgcgc
     1621 taggtatgcc gaacattaat gacgtaaaag attcatttac tatcaatgcc gctgtaacat
     1681 ggacacttgt atatgaatgg ttcttttatt tttctcttcc ggtaatttcc gcgctcataa
     1741 aaagaaaagt cagtatttac atggttatga ttagcgcaat atcgctattt gttttcattt
     1801 tatttttcag taaaatacac atagcatcat ttctttttgg gctcctggca tttttgctaa
     1861 ataaatcaaa gatagtcaat ggaattgcca aagcaaaagt aacaccaatt ataataactg
     1921 cgataatggt ttttgaaatg acgtatttca aaacaactta cgcaccgcta ccactgattc
     1981 tttgtggaat aacatttatc attatcgcat caggttgtga tttgtatgga atattaagat
     2041 taaatataac cagaaagcta ggagaaacta cctacagcgt ttaccttctg catgggatat
     2101 tcctttattg cctaatgacg tggattattc ctaataatta cactgaaaat acatttatca
     2161 tactggtaag cacaactgca tttcttatta cgttcacatc atgcctaact tttaaattaa
     2221 tagagacgcc attcatcaaa ttaacaaaac aaaccacaac tttagtaaaa gaattaatac
     2281 ccacattaac aaacaacaat caataaacaa taaacataag ccgccctaca tatatgtcca
     2341 aatggggcgg cgaatgtatt tatattagtc tggtaatgct aaatgcaaac gaactggaga
     2401 ttcctgcccc gctaagcgta ttgcgcaggg tcaccctaaa tgtatctacg ccaatataag
     2461 taacatcaca actaactgaa tcaggcgcag atataggtgt tatctgcaca atataaccgc
     2521 tagcaactgc tgcgagcgga tgagaaaagc taacatcata cacacctgta gaaaccacgt
     2581 taaccgatga aacaccagta gcaacgtcga tactaccatt ggcgttaatt tttcccgagc
     2641 gtgtgactga caatgcagaa gccgatgtta ccttactggt tgtttctgta aaaacacacc
     2701 caatgtcaac tacctcgcaa ttagctcctg ttatccagta tggtcgttct gcacttggct
     2761 gataatcccc tgcgctctgg aacgttgtgc cgttgagtat gactgtaact tttcccccac
     2821 ctgagcttgt tacttgaata ttcgtgtgcg tgtatcttgc actgctcacg cgatgaaaat
     2881 ttccaccgtt tataaccaca gttacagggc gcgtcccagt attatctata gcaaggtctg
     2941 cccccccggc attagcctca aagtatgggt tgtttaatac caaagcgcac gaaccgttaa
     3001 ggccatttat agctaattgc gcaccacctg tactggaatc cccctgagta ccatttcctt
     3061 caca
//
LOCUS       DAJTPG010000222         3060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTPG010000222 DAJTPG010000000
VERSION     DAJTPG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO230_004082"
     CDS             complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="OO230_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5693610.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     H"
     gene            complement(919..1014)
                     /locus_tag="OO230_004083"
                     /pseudo
     CDS             complement(919..1014)
                     /locus_tag="OO230_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="OO230_004084"
     CDS             complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="OO230_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5693611.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="OO230_004085"
     CDS             complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="OO230_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5693612.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2111..3060)
                     /locus_tag="OO230_004086"
                     /pseudo
     CDS             complement(2111..3060)
                     /locus_tag="OO230_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          764 a          743 c          736 g          817 t
ORIGIN      
        1 atgggatgat aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat
       61 gctttccggg atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt
      121 atttccctca gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt
      181 aagagcctgt gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa
      241 taacgaatcg ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc
      301 cagcgcagga ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt
      361 tagcgtaaca ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt
      421 tatctgagct ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc
      481 atcggaatta ttgataagac attctttaag tcgggagaca gcctcatcac gctcttcacc
      541 ggggagcgct tgtttttccc atttatccca tgctgaaaag taatcagcat atgtaccgga
      601 aatagtgtta taaaaagaat tttggggcaa tgaaaagtta ttatttatcg gtaacatgat
      661 gggcatccag ttaaagccgg tggacaaatg gctttgttaa aagaaataag taggagtttt
      721 tatgaagttc attccatgaa atgaacttca gcactttttt attataataa ggataaacaa
      781 aattggctgt aaaaaaacga tactgtttca gttaaccgtg aatggcctgt ttgcaccgta
      841 ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacggt aagcggctcg
      901 tcagaaccgt attgatattt actgagaggt cagatcaact ttccaaggca acagatcgcg
      961 tacccggttt gccggccagt cctggatatg ctcaatgacg taacgcagcc attttgtcaa
     1021 cgccacgatt tttgacccta gcctgacatt tatattcccc agaacatcag gttaatggcg
     1081 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacgaagatc
     1141 ggcacactgg ccatatcagt ggtcatcatg cgccagcttt catccccgac atgcaccacc
     1201 gggtaaagct cacgggagac tttatctgac agcaggcggg cactggccag ggggatcacc
     1261 atccgtcgtc cgggcgtgtc aataatgtca ctctgtacat ccacaaacag acgataacga
     1321 ctctctcttt tataggcgta aaccttaaac tgcatttcac cagtccctgt tctcatcggc
     1381 aaaagagccg ttcatttcaa taaaacgggc gatctcagcc atcccctgct gattttcagc
     1441 ctgccagcgt tccgcacgca gacggcgggc ctctttttgc atggccgtat ttaccaggcc
     1501 tgagatattc acgtttgcgg atttaagtaa ctgatagctg tcactgtcta tagtgacagt
     1561 aatacgctgc ttcatgataa gccctcaaaa acatacttat gatatgtata tgagtatata
     1621 ttaatggtac aaaccatcaa tatccataaa aagtacacca ttaattcgct ataagttttt
     1681 aatacaattt tttctgctgc gattttttta tactataatt agcatactta taacaccgta
     1741 agtaaattac atgaaagcag gcacagcgtg atccgatttt tctgcgtata acgattttcc
     1801 ggaagtgtaa tacggttgcg ccccgggccg ggcacccttt tctgttgttg tgtacgggcc
     1861 cacagagaat ggtaccggag gcgggctcag aagtactgag tcgggtgatc gcgtcaggcc
     1921 gcgaagtact gacacgggta ttggtgactg gtgatgaagt actgccccgg gtatgatttc
     1981 aggctgattc aggttctgat tctctgtcag tccgcgtggt tgctggcctg gctgatttcc
     2041 tgttgtcagg cgttcaacaa ccgactgcag gttatagccc ggttcacgaa cacctgacgg
     2101 caggtatcac tcacccggtg aaggtaaatc cctcaagagg cagcaactct gccagtcgct
     2161 cctccggcca ttccggcaga cgcgtcagga cgtccgtcag ccaggcatgt ggctccagac
     2221 cattacgttt tgctgtttcc agaaggctca ttatctgtgc cgcccggttt ccggccatca
     2281 gtgaaccggc gaacagccac gctttccgtc ccataacaac cgggcgaatg gcacgctcgc
     2341 agatattgtt gtccaggggc acagctccgt cacccaggaa gcagctcagt tcatcgcgcc
     2401 ggttcagaat atagttaatc gccttattta atggcccgtc gggcggacag cacggctcct
     2461 gctcttcaag ccaggcaaac aggtcattca ctatcggccg ggagtaccgt tatcgccact
     2521 gccgtatttt ttccacaggg cgctcacgga tgagtttctc aatgcggtaa agacccgcga
     2581 tttttctcag ctccacgcct gcacggggct ccctgctggc cttatacaga tttgcgaagc
     2641 cgcgccctgc gtgactccag caacaggctg atgtgatgcc ggggtgatta tccgccagag
     2701 atttgtacac gctgtatccg tcactcacca gcgtactgcc acaccagcac tgcagccagg
     2761 tctcagggta tctgagggcc cgtccggtct gcgagtcaaa acagacgacc ggcggaccgc
     2821 ttctttcacc gctgacgtaa gcacacagcc agccggagca ggatttaccg cctttccggg
     2881 tatcaaggag cctcagggac gtttcatctg catgaataac atcccgtgtc agaagctcat
     2941 ggcgtagcag tttcgccagc ggggccagtg cagctccggc aacaccgacc atatccgcca
     3001 ttgtactgac ggggagctcc acacccattc gggagaaaat gtgctgctgg cgatacaggg
//
LOCUS       DAJTPG010000223         3052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTPG010000223 DAJTPG010000000
VERSION     DAJTPG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..187
                     /locus_tag="OO230_004087"
     CDS             <1..187
                     /locus_tag="OO230_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5693613.1"
                     /translation="GGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGV
                     NVFIQDVPGDQKEQIPD"
     gene            226..1029
                     /gene="agaC"
                     /locus_tag="OO230_004088"
     CDS             226..1029
                     /gene="agaC"
                     /locus_tag="OO230_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5693614.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1019..1810
                     /gene="agaD"
                     /locus_tag="OO230_004089"
     CDS             1019..1810
                     /gene="agaD"
                     /locus_tag="OO230_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5693615.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1811..2566
                     /locus_tag="OO230_004090"
     CDS             1811..2566
                     /locus_tag="OO230_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5693616.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2967..>3052
                     /locus_tag="OO230_004091"
     CDS             2967..>3052
                     /locus_tag="OO230_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5693617.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIV"
BASE COUNT          792 a          683 c          723 g          854 t
ORIGIN      
        1 aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg aagggaaaaa
       61 gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac gttttattaa
      121 acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag aacaaatccc
      181 tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca tgaaataacc
      241 ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga tttttggctg
      301 gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc tattctcggt
      361 gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc cggattaacc
      421 cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac caccgtcatt
      481 gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc gtttagtttg
      541 ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat gaccaaagcc
      601 gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg gacaacgatg
      661 ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct ggcacaaggg
      721 gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt tgaagtggct
      781 ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt caaagcgcaa
      841 tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt cagcaacctg
      901 ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa tgcgaaatcc
      961 cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga ctacagcaat
     1021 gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt cgctgctgca
     1081 agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga tgttgccgat
     1141 cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga aagataacct
     1201 cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat taatttcgat
     1261 ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac tgtttggccc
     1321 aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta tggcgggaat
     1381 ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt tcgccgttta
     1441 cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag tcggcgtgaa
     1501 ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa ccatcctcgg
     1561 gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg tgacatcgtt
     1621 tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata aagtcttccc
     1681 gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg tgaaaaaagc
     1741 gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt ccgcattcgg
     1801 cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta aggaatttca
     1861 tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac gagccagcga
     1921 atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg cgaccggagc
     1981 cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc aggttgatat
     2041 cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa cgatgcctgg
     2101 cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac gtgaagacca
     2161 gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg taacgaacct
     2221 gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa acggtcatct
     2281 tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc aacttgatgc
     2341 cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc gtgggatcac
     2401 cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga ctggcgaagg
     2461 aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta tcccggcttc
     2521 atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag atgaaaaatt
     2581 tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa taacatttga
     2641 ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta attttagata
     2701 tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc ctggcgcagc
     2761 aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata taataaaaac
     2821 ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt tcaatgaaat
     2881 atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa tatgattatg
     2941 tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct attgtgggct
     3001 tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt tt
//
LOCUS       DAJTPG010000224         3052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTPG010000224 DAJTPG010000000
VERSION     DAJTPG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..507)
                     /gene="ychE"
                     /locus_tag="OO230_004092"
     CDS             complement(<1..507)
                     /gene="ychE"
                     /locus_tag="OO230_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5693618.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFR"
     gene            984..>3052
                     /gene="adhE"
                     /locus_tag="OO230_004093"
                     /gene_synonym="adhC"
     CDS             984..>3052
                     /gene="adhE"
                     /locus_tag="OO230_004093"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5693619.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPAS"
BASE COUNT          835 a          753 c          702 g          762 t
ORIGIN      
        1 gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag
       61 atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc
      121 cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg cggtttctga
      181 tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat
      241 cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg
      301 tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc
      361 tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac
      421 gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac
      481 gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg gataatgtta
      541 gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct
      601 cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa
      661 tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg
      721 ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg
      781 agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa
      841 actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcattggtta
      901 agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag
      961 tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg
     1021 tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca
     1081 aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg
     1141 ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt
     1201 ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg
     1261 acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga
     1321 ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag acccgtaacg
     1381 ccattatctt ctccccgcac ccacgtgcaa aagatgcaac caacaaagcg gctgatatcg
     1441 ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac
     1501 cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga
     1561 ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag
     1621 gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt gcagttgcat
     1681 ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg
     1741 ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggcggctatc
     1801 tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat ggtgcgctga
     1861 acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac
     1921 cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg
     1981 cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg
     2041 tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca
     2101 ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacagctc
     2161 gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac
     2221 tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg
     2281 ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt
     2341 ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg
     2401 aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca
     2461 atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct
     2521 tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa
     2581 actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga
     2641 agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct
     2701 ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga
     2761 tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg
     2821 acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg gatatggcga
     2881 ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg
     2941 acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg
     3001 gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc ta
//
LOCUS       DAJTPG010000225         3039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTPG010000225 DAJTPG010000000
VERSION     DAJTPG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..546
                     /locus_tag="OO230_004094"
     CDS             <1..546
                     /locus_tag="OO230_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5693620.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
     gene            958..1326
                     /locus_tag="OO230_004095"
     CDS             958..1326
                     /locus_tag="OO230_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693621.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            1757..2272
                     /locus_tag="OO230_004096"
     CDS             1757..2272
                     /locus_tag="OO230_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693622.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            2262..2762
                     /locus_tag="OO230_004097"
     CDS             2262..2762
                     /locus_tag="OO230_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693623.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
BASE COUNT          726 a          793 c          766 g          754 t
ORIGIN      
        1 tatcagttca cacgacacac ggctcacacg acctctgagg cccgtagatg ggcgtctctg
       61 gtgtctgtgg tcactggcgc tcggtctgct gaggtctgcc acctgactaa acgtgacatt
      121 gtaaccttgg acaatggact ggtctgtatc gacatcaatg aagatgggga cgggaagtct
      181 gtgaagaaca aacattcagt ccgacttgtg ccgttaaccg atggcgctta tgggtttgac
      241 ctgacgtcct tcctgtcgtg ggtagacatg cagcctgatg agggtcctct cttcgggatg
      301 actccgagcg cctactctag ctggtttaac tcaagggtcc tgactgaggc cctaggagac
      361 tctcaggatg tctctctcca cagtctgaga cattggctgg cgacccgaat gaaagagcgt
      421 ggagtcaatc tggtggacgc tcaaggcatc ttaggtcaca gcagtcagtc aattacgtat
      481 gacctgtacg ggaaaggaca cgctgtgggt aggctggctg atgtgcttaa gacagcttta
      541 ctttaagtag ggagacccaa ggtggagacc atatacggct tgatggttga gaccttgggt
      601 cactgaagtc tgactgaagt tagggagggt gataataatc ataatccctc ctgactatag
      661 gcccactggt ccgccttcgg ctgtccgact ctaagtctgt ctgtggtctt atttaaactg
      721 cgtcctaaac tccagtccct ccaaattcct atggtctcct cttgacattc ctgtttaact
      781 gtgagtcgcc tctggtctct ctttatatat acttccttaa ttcctgaggc gtctttagtc
      841 cttgatattc ggttcaacct gtgagtcaca tcctgtcgcg ctactgtatg catcaccagt
      901 gatggacttg attatcacta taggggaacc aactaattca actacaggag atactctatg
      961 gcacgaccaa tcgcaactca cgacaacacg ttcaccaaag cctacttgca gcagcactgc
     1021 ggggacctgc tgtcattcga tggtcagggc gacctgagtg gttggctgga tgatgtcctt
     1081 actggtgctg gtcgcctgaa cgaatcaatg gcaagcaata ccaagcctgt cagcccatac
     1141 ttgattctga cccaactact aacgcacgac acattaactg taagcgctgt gcaggagtcc
     1201 ctgagccgta agcgtgttgc cttgggtgaa cctatggttt caactcgcta cgctcgctac
     1261 gtctatgcta ccgtcgtcag cgccagtaaa tccgtccagt accacgccag taaagctggt
     1321 tcatgattac ggaccctaaa ctatcactat agggagacca gcctccgcct ctgagtctga
     1381 tgcgtcagaa catgcgagca tctggtcaac cgacattaag tatctctcct aagtaatcaa
     1441 ggcccgtgac cgcctctgac agacctcctg tatcgacctc cagctcgtta gcgttcttta
     1501 gtcgtctcgg ttagtagctc gtctactcac tacattggct taagtctatc accgctcacc
     1561 atctggagtc cttacagctc gtcctgacat gcggtcgcac ctttatacgt acatggagaa
     1621 catggtcact ccagaccgtc aacatttaac cattcacttt cgttaagcac tttctgatgg
     1681 gtccgtttag gatgcgtctg aatggtgcgg actccatgtg gagacagaaa gtgctttacc
     1741 aaaggagcat ctttgtatga ataccgaatt accagtaact aaccgccagt tcttaatcat
     1801 cctgctgacc aatgcagagt ccagtgtcga ttgctgtcgc tataacttag acctgctgaa
     1861 gttatcccga tggtatgccc aggcggaaga ctgggactat gagaaaatcc gggagatgta
     1921 cgcggagcct atccagtcat acctccagac acacaccgag gaagagctga agcgtggcct
     1981 gcaggacatc ttctatttca acaatcctta cacctgtgag ggtgactcag aagagatgga
     2041 ttggatagcg tcacgctctg atgccgactt gaagcacatc tctaacgcga tgatgcaggt
     2101 ggagagcaaa tggtcccgcc gccttcaggt cctaactgaa gattacgaaa acggcacctt
     2161 tgataagctg gtcccacctg atgccgaacg ccttcaggcg aaagctgacc aagaacagcg
     2221 cttcaagtcc tttgccgaag ctctggcggg aggtgcctct tatgacttct gacaataagc
     2281 aggaatacac cattcatatc ttcggtcagg acatcaccag tcaggacgga ttgtttcgac
     2341 tggatgagat acgccgggta ggcattcaga ttggtaaggt agaagataac cgtagtaccg
     2401 aggttgctcg cttcatgagg accaaggctg gtagaagtct aaccaaccca gaaaacgggt
     2461 ttgttaaaaa gattaacatg ggtcacttag ggacctcgtg gctggctgac ccagtagccg
     2521 ctatagagtt cggtcggtgg ttggaccttg ggtacggact ggcagtaacc cgagccttcg
     2581 tgtctatgac ctccagcgcc accgtgcagt ctgctgtagc gtctgctgag gacatcaagc
     2641 cggagaccaa ggaattcatc cagcgtcatg gcacaggcct ctacatcgcc ggacgtgaag
     2701 tctcacaggc tgagtattca gactatcaag ctgtgaccgc tggtcgctgt gagtggaggt
     2761 aagtaatcat ggctatgatt gaccctcgta cacctatcgg caaagcgacc cttcgttacc
     2821 gtggcctgcc cacacgtcat ctgctgtctc tgctgagact tggagtcgaa gaccctgagc
     2881 gcccttacta cagtcgagac gagctgatag ctatgctggt ggaccgtgat ttagacaatc
     2941 agttacgccg agcctttgct aagcagtcct gaccatacca aacctaattt taaacctttt
     3001 gagatgaccg atgcggggag acccgtaggc gctactgcg
//
LOCUS       DAJTPG010000226         2950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTPG010000226 DAJTPG010000000
VERSION     DAJTPG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..157
                     /locus_tag="OO230_004098"
     CDS             <1..157
                     /locus_tag="OO230_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5693624.1"
                     /translation="VERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDR
                     LKYKGAV"
     gene            494..2107
                     /gene="mppA"
                     /locus_tag="OO230_004099"
     CDS             494..2107
                     /gene="mppA"
                     /locus_tag="OO230_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5693625.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2158..>2950)
                     /gene="ynaI"
                     /locus_tag="OO230_004100"
     CDS             complement(2158..>2950)
                     /gene="ynaI"
                     /locus_tag="OO230_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5693626.1"
                     /translation="ICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKI
                     TIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIG
                     DWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTT
                     IGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTV
                     WAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          832 a          653 c          689 g          776 t
ORIGIN      
        1 ggttgagcgg gctattaaag aaaaaaaact gatacgtgtt ttggataaat tcactccaga
       61 tttacccggt tatcacctgt actatccaca ccgtcgacat gctggctcgg cattctcatt
      121 atttatagat aggctgaagt ataaaggtgc tgtttagcac tacttgctga tacattaatt
      181 taattcttct cttaacgtat tctcagttcc tttcaacgtt ttggttattt tttattcttc
      241 gtacaatggc gacagatgct gattatgata ccgaaaactg gtttgaacgt gcgaagcccg
      301 aacgtagtgt tcggtgttct atgtgctttg ccacattttg gcgtctatta tttacactaa
      361 atctgatttg atatattgat acttaaaaca tttgatgctt cctttgtcac ttttttgatg
      421 gaagttgttt gcatttcttt aaggcgaaac aaataattac gcatcaattt taatgtcggt
      481 tagagggaaa cttatgaagc actctgtttc agttacgtgt tgtgcgctgt tggtcagcag
      541 catttctctt tcgtatgctg cagaagttcc gagcggcaca gtactggcag agaagcagga
      601 gctggtgcgc cacattaaag atgagcctgc gtcgctggat cccgctaaag ccgtgggcct
      661 gccagagatt caggtcattc gcgatctgtt tgaaggtctg gtgaatcaga acgaaaaagg
      721 ggagattgtc cccggcgtcg cgactcaatg gaaaagtaat gacaaccgta tctggacttt
      781 taccctgcgc gataacgcaa aatgggcgga tggcacaccg gtaacggcgc aagattttgt
      841 ctacagctgg caacgtctgg tggacccaaa aacattgtcg ccatttgcat ggtttgccgc
      901 gctggcggga atcaacaacg cacaggcgat tattgatggt aaagctacgc ctgatcagct
      961 tggcgtcacc gcagttgatg cccatacttt gaaaattcag cttgataaac cgttgccgtg
     1021 gtttgtgaat ttaaccgcta actttgcctt cttcccggtg caaaaagcca acgtagaaag
     1081 cggtaaagag tggacgaaac ccggaaatct gatcggcaat ggcgcttatg ttcttaaaga
     1141 gcgcgtagtc aatgaaaaac tggtcgtggt accgaatacc cattattggg ataacgccaa
     1201 aacggtgctg caaaaagtga ccttcctgcc aattaatcag gaatccgcag ccactaagcg
     1261 ttacctcgcg ggggatattg atatcaccga atccttcccg aaaaatatgt atcagaagct
     1321 gttgaaggat attccggggc aggtttatac gccaccgcag ctcgggacct attattatgc
     1381 ctttaacacg caaaaagggc cgacggcaga tcagcgcgtt cgcctggcat taagtatgac
     1441 gatagatcgc cgcctgatga ccgaaaaagt gttggggacg ggcgaaaagc cagcgtggca
     1501 ttttatacca gatgttaccg ccggatttac gccggaacct tcgccgtttg aacaaatgag
     1561 tcaggaagaa ctgaatgcgc aggcaaaaac tttgttgagc gcagctggtt atggtccgca
     1621 aaaaccgctg aagctgacgc ttttgtataa cacttcagaa aaccatcaaa aaattgcgat
     1681 tgctgtagca tcgatgtgga aaaagaacct tggcgtagat gttaaattgc aaaatcagga
     1741 atggaaaacc tatatcgata gccgtaacac cggcaatttt gatgttatcc gcgcctcatg
     1801 ggtgggggat tataatgaac cctccacttt cctgacatta ttaacgtcaa tgcattcagg
     1861 aaatatttca cgctttaaca atccggcata cgacaaagtt ctggcccagg catcgacgga
     1921 aaataccgtt aaagcgcgta atgccgatta caacgcggca gaaaaaatcc tcatggagca
     1981 agcaccgatt gcaccaattt atcaatatac caatggacga ttaatcaaac cgtggctgaa
     2041 aggttatccc attaataacc ctgaagatgt agcgtacagt cggactatgt atattgtgaa
     2101 gcattgatgt gatgggaact ggcgttaccc ttgtggataa cgccagtgat gtctgattta
     2161 gcgcccctgt tccggcggtg taatgttatc catatacagc gtctggctgg ggaaggcaaa
     2221 gtccgcgccg tgtgactgta caatatcgat aatcttcaaa taaacgtctt gctgtgcagc
     2281 aagccactca gcccataccg tggttttggt aaagcaataa accataatat tcaatgacga
     2341 gtcagcaaac tggttgaaat aaaccagtaa ggtttgtcgc tggtcgatgg ccgggtgatt
     2401 tttcagcatc tcacgtacgg cttcgacaat aacgcccact tttgccgcat cctcataacg
     2461 taaaccaatg gtcgtggtaa tgcggcggtt ggtcattcgt cctgggtttt ctacgctgat
     2521 cgacgaaaac agcgagttcg gtacgtacaa tggacgatta tcaaaggtcg taattttggt
     2581 aattcgccag ccaatttccg ctactgtacc ttcgatattt ctgtccggtg aacggatcca
     2641 gtcgccaata ctgaaaggac ggtcgaaata gagcatgatc ccggaaaaga aattactcag
     2701 aatatcttta ccggccatac cgacagccag accaccaata ccaccaaagg tcagcaagcc
     2761 agaaaggctc atgccgaaat gttcgccata aagcagaaca agcactacaa taatggtgat
     2821 tttgataatg cgcgacataa tccgcgcact ggtgatatcg cgaccttttt taatctgctg
     2881 tttttcaaac tgattaatca gcagaaatag cttaatcgtc agaataaccg caatcaggga
     2941 cgtacagata
//
LOCUS       DAJTPG010000227         2913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTPG010000227 DAJTPG010000000
VERSION     DAJTPG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            156..434
                     /locus_tag="OO230_004101"
     CDS             156..434
                     /locus_tag="OO230_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693627.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            436..1494
                     /locus_tag="OO230_004102"
     CDS             436..1494
                     /locus_tag="OO230_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5693628.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            1495..1860
                     /locus_tag="OO230_004103"
     CDS             1495..1860
                     /locus_tag="OO230_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5693629.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            1857..2540
                     /locus_tag="OO230_004104"
     CDS             1857..2540
                     /locus_tag="OO230_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5693630.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            2739..2814
                     /locus_tag="OO230_004105"
     tRNA            2739..2814
                     /locus_tag="OO230_004105"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2772..2774,aa:Ile,seq:cat)
     gene            2816..2892
                     /locus_tag="OO230_004106"
     tRNA            2816..2892
                     /locus_tag="OO230_004106"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2850..2852,aa:Lys,seq:ttt)
BASE COUNT          747 a          703 c          793 g          670 t
ORIGIN      
        1 gggagaaatc cctcgccacc gctgatgtgt caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg ggttttgttt tttctggtcg ttctggttta caatccatcc gtcagcctga
      121 acaactggca cctgctgcgc cagcagagaa aacagatggc gcacgatacc aaattttaca
      181 attcggataa ctctgccgcc cctgccagca ggcacgggcg gcgttctcat gcattcaaat
      241 ctgacgggta tcagcacgac ccctgcaccg aagaacaggc tgaatggctg attcagcgct
      301 accgcagaca cggatacgag attaagaaag ccctcagtct cgattatcgt cactggataa
      361 tcttcgtcag gctcccttat tccgaacggc caccgcgtcc gtcctgcaca ttccagcaac
      421 ggatctggag gtaatgtgcg ggtattactt cgacctgttc tggttccgga actcgggctg
      481 gtggtcctta agcccggtcg tgaatccatg ccggtattcc acaatacccg ggtactggtg
      541 gagccggaac cgaaaagcat gcgtaatctg ccgtccgggg tcgttcctgc cgttcgccag
      601 ccgctagtgg aagacaaaac attgctgccg tttttcagta acgcacgggt gattcgtgct
      661 gctggtggtg ctggcgcatt gtctgactgg ctgttgcgcc atattaaatc ctgccagtgg
      721 ccacacggcg attatcatca cagcgaaacc gtcattcacc gttatggtac cggcgcaatg
      781 gtattgtgct ggcactgcga caaccagctg cgtgaccaga catccgaatc actcgagcaa
      841 cttgctcatc aaaacctgtc agcatggatg attgacgtca tcggtcacgc aataagcggt
      901 acgcaggagc gtgaattatc tctggctgaa ttatcctggt gggcggtccg caatcaggtg
      961 gcggacgcgc taccggaagc ggtattacgt cgttcgctgg ggttgcgtgc ggaaaaaatc
     1021 cgctcaatgt accgtgaaag cgacatcgtg ccgggagagc agaccgccac cagcatactg
     1081 aagcagcgca caaaaaatct tgcgccgctg cctcacgccc accagcaaca gaatccacca
     1141 caggaagaga cggtggtcag cattgccgtt gatccggaat ctccggctca gtatctccag
     1201 cgccagaaac cacaacggga agagatgcct gtatacacgc gctgggtaaa aacgcagaaa
     1261 tgcatgacgt gtggcaatca ggcagatgat ccgcaccaca tcattggtca tggactggga
     1321 gggatgggaa caaaggctga cgatttgttt gttattccgc tgtgtcgtaa atgccataac
     1381 gaactacacg ccggggtaaa agattttgaa gaaaaacacg gtagtcagtt gttgttgctg
     1441 attcgttttt taatgcacgc gagaaattcg ggtgttctga agtggaaagc ataaatgacc
     1501 gaacgcatag aatttgtttt gccttacccg ccaacggtga acacctactg gcgacgtcgt
     1561 ggcagcacat attttgtatc aaaagccggt gagcgttatc gccgtgatgt ggcgctaatt
     1621 gttcgccagc agcgactgaa attaaacctg tccggaaggc tggcgataaa gattattgca
     1681 gagccaccgg ataagcgccg tcgtgacctg gacaatatcc tgaaggcacc gctggatgcg
     1741 ctgacgcatg ctggactact tatagacgac gagcagtttg atgaaatcaa tattgtgcgc
     1801 ggtcagctcg ttcctggtgg gcggctgggg ataaaaatca cagaactggg gtgcgcatga
     1861 ataaccagta tttacagttt gtgcgtgagc agctcattat cgccaccgct gatttgagtg
     1921 gggcaacaaa aggtcagctt gaagcctggc aagagaatgc catgttcgat acagggcgtt
     1981 acaggcgtaa aaaaatccgg taccgcgatg aagtgactgg aaaaatgata acgcgggata
     2041 atccaccaat cccgggaaag caatcgctgg cgaaggggac gtcaattcct ctggtcagtc
     2101 cggttgagtt ttcgacatca tcgtggcggc gggctgttct gtctcttgaa gagcactata
     2161 aagcctggtt gttgtggtgt tacagcggga gtatttgttg ggaatatcag atcgcgataa
     2221 cacagtgggc gtggaatgaa tttaatgctc aatccggtac cagaaaaatt gcagggaaaa
     2281 cgcaggaacg cctgaaaaaa ttaatctggc tggcggcgca ggcagtaaaa gcagaacttt
     2341 ttggtgggga aggttatgaa taccaggagc tggcattact ggcgggagtg acaactaaaa
     2401 actggtccaa aacatttact cgtcactggg ttgcaatgaa acacattttt caccgactgg
     2461 atagtgaggc tttattgttt gtaatgagaa cacgttcaaa aaaaagacgg cattttcaaa
     2521 gcaaagtgtt gcaaaagtag attgaaaggc atatattcat gcaaatctga tactttgccg
     2581 attttgtacg tgatggcaaa agcaaacaaa acccgcccac aagcgggttt tttgtgccac
     2641 ttatctcgga tagacatggt gaatgcgctg gtggaggaga taagggtgat ttttgaatgc
     2701 ttgcaacatt gatttcgtaa cgttattatc ctgcgcccgg ccctttagct cagtggtgag
     2761 agcgagcgac tcataatcgc caggtcgctg gttcaaatcc agcaagggcc accaaccgcc
     2821 actagctcat caggaaagag cgtcaaccct ttaagttgag tgtgcgaggt tcgagtcccc
     2881 ggtggcggtc cagtgccgac ttggctcagt agg
//
LOCUS       DAJTPG010000228         2890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTPG010000228 DAJTPG010000000
VERSION     DAJTPG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="OO230_004107"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="OO230_004107"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5693631.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="OO230_004108"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="OO230_004108"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5693632.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="OO230_004109"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="OO230_004109"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5693633.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="OO230_004110"
     CDS             complement(1897..2811)
                     /locus_tag="OO230_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5693634.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          773 g          690 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc
//
LOCUS       DAJTPG010000229         2881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTPG010000229 DAJTPG010000000
VERSION     DAJTPG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO230_004111"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO230_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5693635.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO230_004112"
     CDS             complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO230_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5693636.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO230_004113"
     CDS             complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO230_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5693637.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
BASE COUNT          785 a          749 c          704 g          643 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatgt
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1501 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1561 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
     1621 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
     1681 gaagatcagg ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
     1741 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1801 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1861 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca ttgccgagac
     1921 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1981 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg
     2041 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     2101 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     2161 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtt
     2221 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2281 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat tgctctcgcc
     2341 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgacgatg gcgaaaatcc
     2401 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2461 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
     2521 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2581 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2641 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2701 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2761 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt
     2821 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatag gtaggtaccg
     2881 g
//
LOCUS       DAJTPG010000230         2847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTPG010000230 DAJTPG010000000
VERSION     DAJTPG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            37..1905
                     /gene="kup"
                     /locus_tag="OO230_004114"
     CDS             37..1905
                     /gene="kup"
                     /locus_tag="OO230_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5693638.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2072..2491
                     /gene="rbsD"
                     /locus_tag="OO230_004115"
     CDS             2072..2491
                     /gene="rbsD"
                     /locus_tag="OO230_004115"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5693639.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2499..>2847
                     /locus_tag="OO230_004116"
     CDS             2499..>2847
                     /locus_tag="OO230_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5693640.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          640 a          683 c          746 g          778 t
ORIGIN      
        1 aagcacacat ttcatatttc aacgaaagac tagtctatga gcactgataa taagcaatca
       61 ttgcccgcga ttaccctcgc ggcgattgga gttgtctacg gcgatattgg taccagcccg
      121 ttatatacac ttcgtgaatg tttgtccggc cagtttggtt ttggcgttga acgcgatgcc
      181 gtgtttggct ttttatcgct gatcttctgg ctgctaatct ttgtggtttc cattaaatat
      241 ctcaccttcg tgatgcgggc agataacgcc ggtgaggggg ggatcctgac gttgatgtcg
      301 cttgccgggc gtaatacgtc ggcgcgaacc acatcaatgc tggtgattat ggggctaatc
      361 ggcggcagct ttttctatgg tgaagtcgtc ataacacccg ctatttcggt gatgtcagcc
      421 attgaaggtc tggaaatcgt cgccccgcag ctggatacct ggatagttcc cctctcaatt
      481 atcgttctca ctttattatt tatgattcaa aagcatggca cggctatggt cggtaagctg
      541 tttgcgccga tcatgctgac ctggtttttg attctggcag gactggggtt acgtagcatt
      601 attgctaacc cggaagtgct gcatgcgctg aatccgatgt gggcggtgca tttcttcctt
      661 gaatacaaaa cggtttcttt tattgcatta ggggcagtgg tgctgtcgat tacggggggc
      721 gaggtgctgt atgctgatat ggggcacttt ggtaagttct ctattcgcct ggcgtggttc
      781 accgtcgtat taccttcctt gacccttaat tacttcggtc agggagcgtt gttgttaaag
      841 aacccggaag cgattaagaa cccgttcttc ctgttggcac cggactgggc gctgatccca
      901 ctgctgatca tcgccgcact ggcgacggta attgcctcgc aggcggttat ctctggcgtc
      961 ttctcattga cgcgtcaggc ggtacgtctg ggatatttgt cgccgatgcg cattattcac
     1021 acctccgaaa tggagtcagg gcaaatctat attccgtttg tgaactggat gctctatgtc
     1081 gcggtcgtga ttgtgattgt cagctttgag cactccagca acctggcggc ggcgtacggg
     1141 attgcggtga ccggaaccat ggtactgacg tctattctct cgactaccgt ggcacgtcag
     1201 aactggcact ggaataagta ttttgttgcg ctgatcctga ttgctttcct ttgtgtcgat
     1261 attccattgt tcaccgctaa cctcgataaa ctgctctccg gcggctggtt gccattgagc
     1321 ctcggtactg tgatgtttat cgtgatgacc acctggaaga gcgagcgttt ccgcttgctg
     1381 cggcggatgc atgaacatgg taactctctg gaagcgatga ttgcttcgct ggagaaatca
     1441 ccgcctgttc gcgtgcccgg gaccgcggtg tatatgtcgc gtgcaatcaa cgtcattccc
     1501 tttgcgctga tgcataacct taaacataac aaggtattgc atgagcgggt gattctgtta
     1561 actctgcgca ccgaagacgc gccatatgtc cataacgtcc gtcgggtaca gattgaacaa
     1621 ctgtcgccca ctttctggcg cgtggtggca agttatggtt ggcgagaaac gccaaacgtg
     1681 gaagaagttt tccaccgctg cggtctggaa ggattaagtt gccggatgat ggaaacctcc
     1741 ttctttatgt cgcatgagtc gttgatcctc ggcaaacgcc cgtggtattt gcgtctgcgc
     1801 ggcaagctgt acttgctgct gcaacgtaat gcgctgcgtg caccagatca atttgaaatc
     1861 ccgccaaaca gggttatcga actgggtact caggtcgaaa tctaacgcca gacgcctcct
     1921 ttcttcataa gggggcgttt ttgttttcat ggttaatcac catgtaaaac gtttcgaggt
     1981 tgatcacatt tccgtaacgt cacgatggtt ttcccaactc agtcaggatt aagctgcgga
     2041 tcagcgaaac gtttcgctga tggagaaaaa aatgaaaaaa ggcaccgttc ttaattctga
     2101 tatttcatcg gtgatctccc gtctgggaca taccgatacg ctggtggtgt gtgatgctgg
     2161 tttacccatc cccaaaagta caacgcgtat cgatatggca ttaacccaag gtgtaccttc
     2221 ttttatgcag gtgctgggcg tcgtcacaaa tgaaatgcag gtcgaggctg ccattatcgc
     2281 ggaagagatc aaacaacata atccgcaact ccacgaaacg ttgctcactc accttgagca
     2341 gctgcaaaaa caccagggaa ataccattga aattcgttac accacgcatg aacaattcaa
     2401 acaacaaacc gcagaaagtc aggcggtaat tcgcagcgga gaatgttctc cgtatgcgaa
     2461 tatcattctc tgtgctggcg tgacgttctg aggccgtcat ggaagcatta cttcagctta
     2521 aaggcatcga taaagccttc ccgggcgtaa aagccctctc gggcgcagcg ttaaatgtct
     2581 atccgggccg tgtgatggcg ctggtgggcg aaaacggcgc gggtaaatcc accatgatga
     2641 aagtgcttac tggtatctat actcgcgatg ccggcacgct tttatggctg gggaaagaaa
     2701 cgacatttac cgggccgaaa tcttcccagg aagccgggat tgggattatc catcaggaac
     2761 tgaacctgat cccgcagttg accattgccg aaaacatttt cctcggtcgt gagtttgtta
     2821 atcgctttgg caaaattgac tggaaaa
//
LOCUS       DAJTPG010000231         2759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTPG010000231 DAJTPG010000000
VERSION     DAJTPG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            406..2058
                     /locus_tag="OO230_004117"
     CDS             406..2058
                     /locus_tag="OO230_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5693641.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2069..2239)
                     /locus_tag="OO230_004118"
                     /pseudo
     CDS             complement(2069..2239)
                     /locus_tag="OO230_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2372..2491)
                     /locus_tag="OO230_004119"
                     /pseudo
     CDS             complement(2372..2491)
                     /locus_tag="OO230_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          824 a          563 c          634 g          738 t
ORIGIN      
        1 tccctccggc agaagcagaa aaagcttatt atgcttctat cggaaacgat gatctggcag
       61 cctgagttca cagataaaac attctccagg aaacttgggg cggttcacgt cagtatcaga
      121 ccatattaat tgctgaaact gataaacatc aaaccttata gaagggtgaa tgctgaaagg
      181 gggctatggt gatcgacgtg atgacaacga caggtaagac cgtgacctga taaacctgaa
      241 ttgtgtccta gacataaagt ccgtgaaggg aataaggctc aggtcagcga atcttatagt
      301 ggcccgcagc tctgactgca ataaggccgg atacagagca gcaactactc gtcgatgtca
      361 gaacaacgga agccttgcaa acggggatgc gttcatacag gaacaatgat accgttaaaa
      421 aaaaacatta cgctaataat gttcaccctg tcattactga caggaaatcc tgctatagcg
      481 tatgaaacag ataaagtata taaaataacc gttctgcata caaatgacca ccatggacac
      541 ttttggcgta acaatcatgg tgagtacggg ttatcctccc aaaaaactct ggtggacaat
      601 atccggcaga aagtaataaa taatggcgga agtgttctat tattatctgg tggtgatatt
      661 aacaccggtg tacctgaatc agaccttcag aaggctgaac ccgatattcg tggaatgaat
      721 ctaattggtt atgatgcaat ggccgtcggt aatcatgaat ttgataatcc tctaaatata
      781 ttacgtcagc aggaaaaatg ggccacattt cctttccttt ctgctaacat atatcaaaaa
      841 agtacaggga ggcgtttatt cagtccatgg aaaattttca tccgtcagaa tttgaaaatt
      901 gcagtcattg gcttgacgac agatgatact gcgaaaacag gaaattctga atattttact
      961 gatattgagt tccgtcaacc tgctgctgag gcgaggagtg tcattgatga acttaatcag
     1021 caggaaaagc cagatattat tattgcggca acacatatgg gtcactatga caatggcgag
     1081 tcaggttcga atgctcctgg tgatgttgaa atggctcgca gtttgccgac tggctcatta
     1141 gcaatgattg tgggaggaca ttcacaagct cctgtctgca tggccagcga taataaaaaa
     1201 cagtggaatt atattccggg gacaacctgt gtaccagata aacaaaatgg catctggatt
     1261 gtacaagcgc atgagtgggg aaaatatgtc ggtcaggctg attttgaatt ctgtaacggt
     1321 acaatgaaac tggttaatta tcagttacac ccagttaatc tcaaaatgag aattaccaga
     1381 gaagatggaa aaacggaatt ttctttttac acccccgaaa ttactgaaga tccacagatg
     1441 ttatccttac tgacaccatt ccagaataaa ggcaaagcac aactggatgt gaaggttggg
     1501 gttgtgaacg gacgtctgga aggggacaga agtaaagttc gttttgttca gaccagtatg
     1561 ggccatctga ttctgagcgc tctgacagaa cgtattgacg cagattttgc tgttgtcagt
     1621 ggtggggaaa ttcgagattc catagagagt ggtaacatca catacaaaga tatactgaaa
     1681 gttcagccat ttggtaatac ggtagtaagt attgatttga cggggaaaga agtagctgac
     1741 tacctagcta cagtggcaca gatgaaaccg gattccggag cctacccaca atttttgaat
     1801 accagctttg tagtcaaaaa aggaaaaatc gaaatgctaa aaataaaagg taaatcggtt
     1861 gaccttaaca aaaaatatcg tatgacaaca tttagcttta atgctactgg tggggatggc
     1921 tatccacgta ttgataatag acccggatac ataaataccg gatttattga tgctgaagta
     1981 cttatcgaat atatccggaa acattcaccg ctggatgctg cttcatatga accaaaaggt
     2041 gaagtcagtt ggcagtgata acgtaattga cgatcatcac gtgttttttg ctgacagtcg
     2101 ctgtgacttt gtgaaagtag tctctgcgta tgtttgcgat atgggaatgc agttgctgta
     2161 ttttgtgttt ctgcttctgc cagttgttgc tgaatttaac cttgcggctt aactgatgtt
     2221 ggagcgctgc cagcttcttc gtcaatttcg ctgattaaag tttattcatt atggcatgat
     2281 ctgcgtaagc ggctcgtcag aaccatattg atatttacta agagctcaga tcaactttcc
     2341 agggcaacag atcgcgtacc cggtttgccg gtcagtcctg gatatgctca atgacgtaac
     2401 gcagccattt ttctggctcc acattgttca gacggcatgt gccgatcagc gagtacaaca
     2461 ccgccgcatg ttcgccaccg ctatcggaac cccgccggag agagtttcac tctccggctg
     2521 ctcacggaac cgtacgtgac actctcgcgt catacggctc ttgatcactc attcgatgca
     2581 cgtagtaaat accagtgggc aaatagcttc ggatactgtt tccttaccag cgccagcttt
     2641 cgctgagcct tccggttcga aagtctgtat ttagactgca tccacttaag caaacgtgac
     2701 tgcattgcac tccacagtcg atagctgaag tttctggacc agaactttcc atagtactc
//
LOCUS       DAJTPG010000232         2703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTPG010000232 DAJTPG010000000
VERSION     DAJTPG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            50..2572
                     /locus_tag="OO230_004120"
     CDS             50..2572
                     /locus_tag="OO230_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5693642.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2703
                     /locus_tag="OO230_004121"
     CDS             2583..>2703
                     /locus_tag="OO230_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5693643.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTL"
BASE COUNT          725 a          583 c          676 g          719 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttg
//
LOCUS       DAJTPG010000233         2699 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTPG010000233 DAJTPG010000000
VERSION     DAJTPG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..138
                     /locus_tag="OO230_004122"
     CDS             <1..138
                     /locus_tag="OO230_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5693644.1"
                     /translation="IYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVTLDY
                     R"
     gene            218..913
                     /locus_tag="OO230_004123"
     CDS             218..913
                     /locus_tag="OO230_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5693645.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            942..>2699
                     /locus_tag="OO230_004124"
     CDS             942..>2699
                     /locus_tag="OO230_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5693646.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLL"
BASE COUNT          789 a          637 c          593 g          680 t
ORIGIN      
        1 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
       61 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      121 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      181 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      241 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      301 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      361 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      421 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      481 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      541 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      601 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
      661 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
      721 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
      781 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
      841 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
      901 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
      961 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1021 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1081 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1141 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1201 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1261 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1321 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1381 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1441 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1501 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1561 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1621 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     1681 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     1741 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     1801 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     1861 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     1921 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     1981 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2041 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2101 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2161 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2221 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2281 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2341 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2401 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2461 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2521 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2581 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     2641 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgtta
//
LOCUS       DAJTPG010000234         2679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTPG010000234 DAJTPG010000000
VERSION     DAJTPG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..535)
                     /locus_tag="OO230_004125"
     CDS             complement(<1..535)
                     /locus_tag="OO230_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693647.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKR"
     gene            complement(1197..2186)
                     /locus_tag="OO230_004126"
     CDS             complement(1197..2186)
                     /locus_tag="OO230_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693648.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2270..>2679)
                     /locus_tag="OO230_004127"
     CDS             complement(2270..>2679)
                     /locus_tag="OO230_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693649.1"
                     /translation="YPLSLAIKTLIPGYLSGLPSTSREAMELLEALAEAGVTIGNFFS
                     NDLMTAYQRRQMNKRAEAERIAKELASQKERTREMFMTEDEWQKELQRREQVKAERRT
                     YGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          696 a          621 c          655 g          707 t
ORIGIN      
        1 gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc gtagacatat
       61 aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg tctgatacta
      121 atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg gtttctgccg
      181 ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt ttctccgtca
      241 acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg tctttggtat
      301 agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga taattaggat
      361 acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg gaattaagaa
      421 tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg ccgcacgaaa
      481 atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa tccatcaatc
      541 gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt attttacctg
      601 agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa atggggaagt
      661 gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga aaatcagata
      721 cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag ggagcctacg
      781 acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat ccagttggtt
      841 gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt aatattccct
      901 ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct caggtcaggc
      961 tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt cagtcaaaac
     1021 tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga agaccacgag
     1081 atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa ctagattact
     1141 agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg ttattgttag
     1201 ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt agccgagcca
     1261 gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc gagaatctca
     1321 aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct ctcgccatcg
     1381 tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa cacgccacgg
     1441 tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc gtcttcacca
     1501 ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc atgggcgata
     1561 atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta cagggcatcc
     1621 atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc tacggtcttc
     1681 agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg aatcacgtta
     1741 tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt ggtcgacttc
     1801 tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg ctcaacggtg
     1861 atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg gacagggacc
     1921 agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct gttacgggtc
     1981 ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac accgttcagc
     2041 atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc gttagtcggc
     2101 agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc aatgccaaac
     2161 tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa tacttgaagt
     2221 ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct tacaagctgt
     2281 ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag cgaccagcgg
     2341 aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg acctgttcgc
     2401 ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg gtacgctctt
     2461 tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc atctggcggc
     2521 gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg ccagcttcag
     2581 ccagagcttc cagtaactcc atcgcctcgc ggctggtgga cggtaagcct gacaggtagc
     2641 ccggaatgag ggtcttgata gcgagcgaca acggatagg
//
LOCUS       DAJTPG010000235         2653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTPG010000235 DAJTPG010000000
VERSION     DAJTPG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..318)
                     /locus_tag="OO230_004128"
     CDS             complement(<1..318)
                     /locus_tag="OO230_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5693650.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAIGNDSNLLIVFYVQI
                     MPDD"
     gene            511..1014
                     /locus_tag="OO230_004129"
     CDS             511..1014
                     /locus_tag="OO230_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5693651.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1060..1476)
                     /gene="rraB"
                     /locus_tag="OO230_004130"
     CDS             complement(1060..1476)
                     /gene="rraB"
                     /locus_tag="OO230_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5693652.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1638..2651
                     /gene="argF"
                     /locus_tag="OO230_004131"
     CDS             1638..2651
                     /gene="argF"
                     /locus_tag="OO230_004131"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5693653.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          752 a          615 c          607 g          679 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccgattgc
       61 gaaatagaaa aaaacaaaaa caagacaact aacaaaaaca ttcccaccgg taattccata
      121 agaaaatcgt tgtccgttaa cttccatatt agcataaaga taacgcttac atttcattaa
      181 tgcccatggt aaatagatac ctagcgttat aatcgttaac aacacattca ccacacaaat
      241 aagaaaatat ctctcacctg taccatgaaa aacaaaagaa tgggacggaa catccatttc
      301 attaataact tgagccataa aaatcccctg aattaaaaaa tccattgata taatttatag
      361 atacagacag taaaatgttc tgccacgtta caatactctt atcagtaata ggctggcaaa
      421 aactttattg ctttataaga cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt
      481 ataattactt tatttttatt gggttaaact atgaataaca ttgcgccgca atcacctgta
      541 atgcgtcgcc tgacgctgca ggataatcct gctatcgcca gcgtcatccg tcaggtttac
      601 gcagagtatg gtcttaccgc cgataaaggc tacaccgttg ccgatccgaa tctcgacgaa
      661 ctgtatcaag tgtacagcca gcctggccat gcttattggg tggttgaata caatggcgaa
      721 gtggtcggcg gcggcgggat tgcgccatta acaggtagtg aagcggacat ttgcgaactg
      781 cagaaaatgt actttcttcc cgctattcgc ggtaaagggc tagcgaaaaa actggcctta
      841 atggcgatgg agcaggcacg agagatgggc ttcaaacgct gctatctgga aacgaccgct
      901 tttttaaagg aagccattgc gctttatgag catttgggct ttgagcatat cgactatgcg
      961 cttggctgca cgggccatgt cgattgtgaa gtgcggatgc tacgcgaact ctaaaaaaat
     1021 gcccgctagt atgtgctatc gggcatttta atcaattaat tagtggcgaa ccccgtcatc
     1081 gtcttcatcg acaaaatctt catcgtcgcc atcttcgccg ttaggatctt caaagtaagt
     1141 gccccaaccg tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg
     1201 ggcatcgatc agatcggcat tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat
     1261 atcaccatct tcaacttcca gctcttctgg atcggtcact tcgtaaccga gtttaaacgc
     1321 ttcaactgcc gctttttcca gagtttccag atcgtctgcg gaaagatggt gttcgatggt
     1381 gtacagcgcg tccggatcgc tgccatcttc cagtaattct tcaataatca aacgtgtttc
     1441 ttcacgctgt tcttccagtt gttccgggtt tgccatggct cattcctctt aatgtcctgc
     1501 cgatactctt attgtcacac accactgaca ttgcctccac ctttgtgaca aagatttatg
     1561 ctttagactt gcaaatgaat aataatacac ataaattgag ttttaattca ttgaggcgtt
     1621 agccacagga gggatctatg tccgggtttt atcataagca tttcctgaaa ttactcgatt
     1681 tcacgccagc tgaactcaac agcctgctgc agttagccgc gaagctgaaa gctgataaga
     1741 aaagcggtaa agaagaagcc aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag
     1801 actcgactcg tacccgatgc tctttcgaag ttgccgcata tgaccagggg gctcgcgtta
     1861 cttatcttgg cccaagcggt agtcagattg gtcataaaga gtcgattaaa gacactgccc
     1921 gcgtgcttgg tcgcatgtat gacggtattc agtatcgcgg ctatggtcag gagattgtcg
     1981 aaacactggc ggaatacgct ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc
     2041 ccacgcagtt gctggcggat ctcctcacca tgcaggagca tttacccggt aaagcattca
     2101 acgaaatgac gctggtctat gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg
     2161 aagctgcggc gcttaccggt ctggatttgc gtctggtcgc gccacaggca tgctggccag
     2221 aagctgcgct ggttacggaa tgccgcgccc tggcacaaca aaatggcggg aatattacgc
     2281 tgacggaaga tgtagctaaa ggagttgaag gtgctgactt tatctatacc gatgtatggg
     2341 tgtcgatggg tgaagcaaaa gaaaaatggg ctgaacgcat tgcattactg cgtgattatc
     2401 aggtgaacag caagatgatg cagttgaccg gtaacccgga ggtcaaattc ctccattgcc
     2461 tgcccgcgtt tcatgacgac caaacgacgc ttggcaaaaa aatggcggaa gagtttggcc
     2521 tacatggcgg aatggaagtg actgatgagg tcttcgaatc tgccgccagc attgtatttg
     2581 atcaggcgga aaaccgcatg cataccatca aagcggtgat ggtcgcgacg ctcagtaaat
     2641 taaataatta ata
//
LOCUS       DAJTPG010000236         2639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTPG010000236 DAJTPG010000000
VERSION     DAJTPG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            174..1715
                     /gene="istA"
                     /locus_tag="OO230_004132"
     CDS             174..1715
                     /gene="istA"
                     /locus_tag="OO230_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCR5693654.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1730..2476
                     /gene="istB"
                     /locus_tag="OO230_004133"
     CDS             1730..2476
                     /gene="istB"
                     /locus_tag="OO230_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCR5693655.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          752 a          623 c          633 g          631 t
ORIGIN      
        1 gatcactcaa taacttccat cgagatcggg taataacatt tgaacagatc gctgaataac
       61 atcgatggag atcacttttg actcattttg ttattcagtg atctccatca atgttattgg
      121 aacttcacag gtgtgttgat ctgtatcttt tgccattccg gtaaaggata cctatgccaa
      181 cagttccaat ttctatgaga aaacttaaag aaattcttag gcttaaatac ggtgttggac
      241 tcagccatcg acaaattggt cgtagtcttg caatctcccc ttccgttgta tccagatatg
      301 ctaatcgggc ggctcaactt ggcataaagc agtggccctt acctacagga tgggatgata
      361 caaaactaaa acatgcgttc cttcagaccc aggttaagat gaagaagcac tctctgcctg
      421 actgggctac agtacaccgg gaactgcgta ataaatgcgt gacgctgcag ctactctggg
      481 aagaatactg tgagcgtaat ccaggcggtt tttacagcta taaccattac tgccggatgt
      541 accgtgaatg gctcaaaacc acttcaccat caatgcgtca ggtacataaa gctggcgaaa
      601 aacttttcgt tgattactgt ggacctaccg ttggcgttac cgaccctgag accggagaaa
      661 taagaactgc tcaggtcatc gtagctgttc tcggggcatc aagttacaca tgggcagagg
      721 ccacctggtc tcagcagctt gaagactggg tgatgagtca tgttcgctgc ttccagtggt
      781 tgggtggcgt tcctgaactt gttgttccgg acaatctgaa aagcgccaca tccagggcat
      841 gtaagtatga tcctgacgtt aaccctacct accagcagat gcttgagcat tataatgtcg
      901 cagttttgcc tgcgcggcca cgtaaaccga aagataaagc caaagctgaa gttggcgttc
      961 aggttgttga acgctggatc atggcccgaa tcaggcatga gatcttctac agccttgcat
     1021 cgcttaatca gcgcattcgg gagttgctgg aaagactgaa taacaaaata atgcagaagt
     1081 tgggttattc acgtgcagaa ctcttcatcc agcttgataa acccgcactg aagcctcttc
     1141 ctgaagccag ttacagttac accctggtga agaaagtcag agttcatgcc gattaccacg
     1201 tggaaatcga caaacattac tactcggttc catgttcgct gttaggccag caactggaag
     1261 catggatctc cggagaactg gtaagactct tcaatcaggg gcaggaggtt gctgtgcacc
     1321 cgcgcaagcg tacttatggc tacagtaccc gcaacgagca catgcctgaa gctcatcgac
     1381 agcatgccac ctggacgcca gagcgtcttc tggaatgggc ggggcacata ggcagtgaaa
     1441 ctcatagtta tgtgcttcat atactgaact ctcgtccaca tccggaacaa agctatcgct
     1501 tctgccttgg actcctgaac cttcataaaa aatacagtaa agccagactt aatgcagcat
     1561 gtgcaagagc tctgaaaaca aaggtatggc gtctgtcagg tattaaatcg atcctggaaa
     1621 aaggtctgga taaacaacct gttcaggatc caaaaccaga tctgttatcc acgatggaac
     1681 acgaaaacgt acgcggcagt gagtattacc actgatacgg gatccaatga tgaatcatct
     1741 ttacgaacaa ctgaccgcac ttaaactcac cggcttccgt gatgcgctta aaaagcaact
     1801 tgctcagccg ggcacatacc aggagctggg cttcgaagaa cgcctgtcat tactgacagc
     1861 agaagaacta acctgccgtg aaaacaggaa ggcagagcgt ctgatcaaac atgcacggtt
     1921 cagacttaat gctgagttat caaagctgga ttatcgtaac aatagagggc tggacagggc
     1981 cctcatccgt tcactcagtc agggaaactg gttaaccctg aaacaaaata ttttactgac
     2041 cggggccacc ggcagcggta aaacgttcct ggcatgtgca cttggtcata atgcctgccg
     2101 acagggatac aaggtctact attatcgcct taaagcgctg atggaacagt gctatcaggg
     2161 gcatgctgat ggaagataca gcaaactttt gaccaggctg aataatagcg atctgctgct
     2221 tctggatgac tgggggctgg aacctctctc atcagaacag cgtagcgacc tgctggaaat
     2281 agtggatctg atgtaccaac gaggctcaat catcgtagtg agccagttgc cggtggaaaa
     2341 ctggtacaaa atgatcggag actccacaca tgcggatgcc atcctagatc gactggttca
     2401 tggcagtatc aagatcgaac ttaaaggaga atcaatgcgg aaaatacaat ctccgttgac
     2461 cgaaggagat cagtgaaggt aatttaaaaa cggttctgtg aaagtgacac gaaccgatct
     2521 ccatcgatgt tactcaccga tctccttcac ggtaatacgc accaggctcc cgcacagtgg
     2581 ctgagctgga atctgaagtt atgcaactgc gtaaggcttt aaatgaagca cgacttgag
//
LOCUS       DAJTPG010000237         2612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTPG010000237 DAJTPG010000000
VERSION     DAJTPG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO230_004134"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO230_004134"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5693656.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2269..2612
                     /locus_tag="OO230_004135"
                     /pseudo
     CDS             2269..2612
                     /locus_tag="OO230_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          666 a          640 c          768 g          538 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactctact cagcacatgg
     2281 cagggcttcg tctatgtggc gttcatcatt gatgtgtttg ccggatacat cgtgtggtga
     2341 cgagtctcat cgtctatgga aacgacattc gtgctggatg cactggagca ggcgttgtgg
     2401 gcccgtcgtc cgtctggcac catccatcac agcgataaag gctctcagta tgtgtcactg
     2461 gcctataagg cgcgactaaa agaagcaaaa ctgctggcat caacagggag tactggtgac
     2521 tcgtatgaca acgcgatggc agagatcatc aagggccttt acaaagcgga ggtaatacac
     2581 cgtaagagct ggaaaaaccg gacagaagtg ga
//
LOCUS       DAJTPG010000238         2585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTPG010000238 DAJTPG010000000
VERSION     DAJTPG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            10..687
                     /locus_tag="OO230_004136"
     CDS             10..687
                     /locus_tag="OO230_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5693657.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            687..1034
                     /gene="tnpB"
                     /locus_tag="OO230_004137"
     CDS             687..1034
                     /gene="tnpB"
                     /locus_tag="OO230_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5693658.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1054..>2585
                     /locus_tag="OO230_004138"
     CDS             1054..>2585
                     /locus_tag="OO230_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5693659.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVR"
BASE COUNT          623 a          712 c          723 g          527 t
ORIGIN      
        1 acaaaaataa tgtccatcat ttttaatgga cactatcgta tgaaacaccg gacctggatc
       61 actgaagctt tacgtcttca ctttgaagaa catttacccc gggttgtggc cgggcgtcgc
      121 ctgggtgtac caaaatcaac agtttgtagt atgtttgtgc gctttcggag agctggcctt
      181 tcgtggcctt tgccctcagg catgtcggag caggaacttg atgcctgcct ttacggacaa
      241 ttttccacgg taccagtcgt acgtcctgaa agcaccgtta tatccgaagc ccccgtggta
      301 aaaaaacgtc cccggcggcc caacttccct tatgagttta aaatcgcctt agtggagcag
      361 tcactgcagc ccggagcctg tgtggcgcag atcgcccggg aaaacggaat caacgataac
      421 ctgctcttca actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat
      481 atgccggcac tgcttcccgt gacgttaacg ccggagccgg ataataaaat cccggctccg
      541 gcacaggaac cagagcagat aaatacaccg tccgacagtc tgtgttgtga gctggttctg
      601 ccggccggaa ctctcaggct taaaggtaaa ctgacgccgg cgttattaca gacacttatc
      661 cgcgaaataa aagggagcag ccactgatga tatctctccc tgcaggttcg cgtatctggc
      721 tggttgccgg tatcaccgat atgcgaaatg gctttaacgg cctggcctca aaagttcaga
      781 acgtcctgaa ggatgacccg ttctccggac acctgttcat cttccgcgga cgccggggtg
      841 accagataaa agtgttgtgg gctgacagtg acggactgtg cctcttcacc aaacgcctgg
      901 agcggggccg cttcgtctgg ccagtcaccc gtgacggcaa ggtgcacctt actccggctc
      961 agttatccat gcttcttgaa ggtatcaact ggaagcaccc gaaacgaacg gaacgcgctg
     1021 gaatccgcat ataacccgtt gtaaagtgag gatatggaca cctcacttgc tcatgagaat
     1081 gcccgcctgc gggcactgtt gcagacgcaa caggacacca tccgccagat ggccgaatat
     1141 aaccgcctgc tctcactgcg ggtggcggct tatgcttccg aaatcaaccg gctgaaggcg
     1201 ctggttgcaa aactgcagcg catgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa
     1261 accgaacgac agatacagga agcacaggag cgaatcagcg cacttcagga agaaatggcg
     1321 gaaacgctgg gtgagcaata tgacccggta ctgccatccg ccctgcgcca gtcttcagcc
     1381 cgtaaactgt taccggcctc acttccccgt gaaacccggg tcatccggcc ggaagaggaa
     1441 tgctgtcctg cctgtggtgg tgaactcagt tctctgggat gtgatgtgtc agagcaactg
     1501 gagcttatca gcagcgcctt taaggttatc gaaacacaac gtccgaaact ggcctgttgc
     1561 cggtgcgacc atatcgtgca ggcaccagta ccttcaaaac ccattgcacg cagttatgcc
     1621 ggagcggggc ttcttgccca tgttgtcacc gggaaatatg cagaccatct gccgttatac
     1681 cgccagtcag aaatataccg tcgtcagggc gtggagctga gccgtgccac actggggcgc
     1741 tggacaggtg ctgttgctga actgctggag ccactgtatg acgtcctgcg ccagtatgtg
     1801 ctgatgcccg gtaaagtcca tgccgatgat atccccgtcc cggtccagga gccgggcagc
     1861 ggtaaaaccc ggacagcccg gctgtgggtc tacgtccgtg atgaccgtaa cgccggttca
     1921 cagatgcctg cggcggtctg gttcgcgtac agcccggacc ggaaaggcat ccatccacaa
     1981 aatcacctgg ccggttacag cggagtgctt caggccgatg cttacggtgg ctaccgggcg
     2041 ttatacgaat ccggcagaat aacggaagcc gcgtgtatgg cccatgcccg gagaaaaatc
     2101 cacgatgtgc atgcaagagc gccaaccgat atcaccacgg aagccctgca gcgtatcggt
     2161 gaactgtatg ccatcgaggc agaagtccgg ggatgttcag cagaacagcg tctggcagca
     2221 agaaaagcca gagctgcgcc actgatgcag tcactgtatg actggataca gactcagatg
     2281 aaaacactgt cgcgtcactc ggatacggca aaagcgttcg catacctgct gaaacagtgg
     2341 gaggccctga acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa catcgcagag
     2401 aacgccttac ggggagtggc cgtaggccgg aaaaactggc tgttcgcggg ttccgacagc
     2461 ggtggtgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     2521 gagccagaaa agtggctgcg ttacgtcatt gaacatatcc aggactggcc ggcaaaccgg
     2581 gtacg
//
LOCUS       DAJTPG010000239         2572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTPG010000239 DAJTPG010000000
VERSION     DAJTPG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(64..822)
                     /locus_tag="OO230_004139"
     CDS             complement(64..822)
                     /locus_tag="OO230_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5693660.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO230_004140"
     CDS             complement(830..1933)
                     /locus_tag="OO230_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5693661.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OO230_004141"
     CDS             complement(1943..>2572)
                     /locus_tag="OO230_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5693662.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJTPG010000240         2569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTPG010000240 DAJTPG010000000
VERSION     DAJTPG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..365
                     /locus_tag="OO230_004142"
     CDS             <1..365
                     /locus_tag="OO230_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR5693663.1"
                     /translation="VATPVYKAAYSGALKTLLDLLPERALQGKVVLPLATGGTVAHLL
                     AVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTHLDTALETFWQALH
                     RRDVQVPDLLSLRGNAHA"
     gene            358..1317
                     /gene="ssuA"
                     /locus_tag="OO230_004143"
     CDS             358..1317
                     /gene="ssuA"
                     /locus_tag="OO230_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5693664.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1314..2459
                     /gene="ssuD"
                     /locus_tag="OO230_004144"
     CDS             1314..2459
                     /gene="ssuD"
                     /locus_tag="OO230_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5693665.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2468..>2569
                     /locus_tag="OO230_004145"
     CDS             2468..>2569
                     /locus_tag="OO230_004145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5693666.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGW"
BASE COUNT          572 a          709 c          712 g          576 t
ORIGIN      
        1 ttgtcgccac gcctgtgtat aaagccgcct attccggcgc gctgaaaacc ctgctcgacc
       61 tgctgccaga gcgcgctttg caaggcaaag tggtgctacc gctggcgacg ggcggtaccg
      121 tggcccatct gctggcggtc gattatgccc ttaaaccagt tttaagcgca ctgaaagctc
      181 aggagatcct gcacggcgtg tttgccgatg actcacaatt aattgattac catcacagac
      241 cccagttcac gccaaatctg caaacccatc ttgataccgc gctagaaact ttctggcagg
      301 cattgcaccg ccgcgatgtt caggttcctg accttctgtc tctgcgaggt aatgcccatg
      361 cgtaacatca ttaaactggc gctggcggga ttgcttagcg tctctacttt tgcggttgct
      421 gcagaatcct cgcctgaagc gttacgtata ggctatcaga aaggcagtat tggtatggtg
      481 ctggcaaaaa gccaccagtt actggaaaaa cgctatccgc aatcaaaaat ctcctgggtg
      541 gagttccccg cgggtccgca aatgttggaa gcgttaaacg ttggcagtat tgatctcggc
      601 agtaccgggg atattccgcc aatctttgcc caggctgccg gggctgattt ggtatatgtg
      661 ggcgtcgagc caccgaagcc caaagccgaa gtgattctgg tggcagaaaa cagcccgatc
      721 aaaaccgtag ccgatcttaa aggtcacaaa gttgcctttc agaaaggttc cagttcacac
      781 aaccttttac tgcgtgcact gcgtcaggcc ggacttaaat tcaccgatat ccagcccact
      841 tacctgacgc ctgccgatgc ccgcgccgcg ttccagcaag gtaacgttga cgcctgggct
      901 atctgggatc cctactactc cgctgcgtta ttacagggcg gcgtgcgggt gctgaaagac
      961 ggcaccgatc tcaatcaaac tggatcgttt tatctggcag ctcgtcccta tgcagaaaaa
     1021 aacggcgctt ttattcaggg cgtactggca acctttagtg aggccgatgc gttaacccgc
     1081 agccagcgcg aacaaagcat cgctttactg gcaaaaacga tgggcttacc ggcaccggtg
     1141 attgcctctt acttagatca tcgccctcct accaccatca aaccggttaa cgccgaggtt
     1201 gccgccttac agcagcaaac ggcagatctg ttttatgaaa accgtctggt gccgaaaaaa
     1261 gtcgatattc gccagcgcat ctggcagccc actcaactgg aaggaaaaca attatgagtc
     1321 tgaatatgtt ctggttttta ccgacccacg gtgacgggca ctatctggga acggaagaag
     1381 gttcacgccc ggttgatcac ggttatctgc aacaaattgc gcaagcggcg gatcgtctgg
     1441 gctataccgg tgtgctgatc ccaacggggc gatcgtgtga agatgcgtgg ctggttgcag
     1501 cgtcgatgat cccggtgacg cagcggctga agtttcttgt cgccctgcgt cccagcgtaa
     1561 cctcacctac tgttgccgcc cgccaggccg ccacgcttga ccgtctctca aatggtcgtg
     1621 cgttgtttaa tctggtcaca ggcagcgatc cacaagagct ggcaggcgac ggcgtgttcc
     1681 ttgatcatag cgagcgctac gaagcctcgg cggaatttac ccaggtctgg cggcgcttat
     1741 tgctggggga aaccgtcaat ttcaacggta aacatattca tgtgcgcgga gcaaaactgc
     1801 tcttcccgcc gattcaacag ccttatcctc cgctttactt tggcggatcg tcagatgtcg
     1861 cccaggagct ggcggcagaa caagttgatc tctacctcac ctggggcgaa ccgccggaac
     1921 ttgttaaaga gaaaatcgaa caagtgcggg cgaaagctgc cgcgtatggg cgcaaaattc
     1981 gtttcggtat tcgtctgcat gtgattgttc gtgaaactaa cgacgaagcg tggcaggccg
     2041 ccgagcggtt aatctcgcat cttgatgatg agactatcgc caaagcacaa gccgcattcg
     2101 cccggacgga ttccgtagga caacagcgaa tggcggcgct acataacggc aagcgcgaca
     2161 atctggagat cagccccaat ttatgggcgg gcgttggctt agtgcgcggc ggtgccggaa
     2221 cggcgctggt gggcgatggt cctacggtcg ccgcgcgaat caacgaatac gccgcgcttg
     2281 gcatcgacag ctttgtgctt tcgggctacc cgcatctgga agaagcgtat cgggttggcg
     2341 agttgctgtt cccacatctg gatgtcgcca ttccggaaat tccccagccg cagccgctga
     2401 atccgcaagg cgaagcggtg gcaaatgatt ttatcccccg taaagtcgcg caaagctaag
     2461 gaacacgatg atggcaacgc cagtgaagaa gtggttattg cgcgttgccc cctggttttt
     2521 accggtgggc atcgtggcgg tgtggcaact ggcctcctcg gttggctgg
//
LOCUS       DAJTPG010000241         2547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTPG010000241 DAJTPG010000000
VERSION     DAJTPG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..98
                     /locus_tag="OO230_004146"
     CDS             <1..98
                     /locus_tag="OO230_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5693667.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO230_004147"
     CDS             156..512
                     /locus_tag="OO230_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693668.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="OO230_004148"
     CDS             605..823
                     /locus_tag="OO230_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5693669.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="OO230_004149"
     CDS             825..1190
                     /locus_tag="OO230_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5693670.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="OO230_004150"
     CDS             1187..1852
                     /locus_tag="OO230_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693671.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="OO230_004151"
     CDS             1852..2217
                     /locus_tag="OO230_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5693672.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          716 a          513 c          684 g          634 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcaga
//
LOCUS       DAJTPG010000242         2486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTPG010000242 DAJTPG010000000
VERSION     DAJTPG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..557
                     /locus_tag="OO230_004152"
     CDS             <1..557
                     /locus_tag="OO230_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5693673.1"
                     /translation="DLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELL
                     MCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSER
                     KVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGAREL
                     NLPSVYGDAAEWNTAELRREMSHS"
     gene            515..1192
                     /gene="bglJ"
                     /locus_tag="OO230_004153"
     CDS             515..1192
                     /gene="bglJ"
                     /locus_tag="OO230_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5693674.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1230..2018)
                     /gene="fhuF"
                     /locus_tag="OO230_004154"
     CDS             complement(1230..2018)
                     /gene="fhuF"
                     /locus_tag="OO230_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5693675.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2159..2395
                     /locus_tag="OO230_004155"
     CDS             2159..2395
                     /locus_tag="OO230_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5693676.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          639 a          548 c          643 g          656 t
ORIGIN      
        1 ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat tattctttaa
       61 tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg tattcccagg
      121 ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa cccattgaga
      181 atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc gccatgctga
      241 cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt ctcactcttt
      301 cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag atagcttcat
      361 tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg cgtcgactgg
      421 caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt gcaagagaac
      481 ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa ttaagaagag
      541 aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga gtttattcag
      601 aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt catttcagga
      661 tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca tgagaagtga
      721 gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc gtacccggcg
      781 tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt cgccatcacc
      841 gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg aactcttttt
      901 gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt acattaacca
      961 aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc gtggctactc
     1021 aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag cgcataaatt
     1081 taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg ccgaagatat
     1141 tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt aatccgcatg
     1201 atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc acattgctgc
     1261 acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc gtcgcgcagt
     1321 accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc aaaaaagagg
     1381 gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc agtgagatac
     1441 cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc tccggtcgct
     1501 tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat tcgctgctgc
     1561 ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca ggcgacgcgt
     1621 ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc tttttcctgc
     1681 gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg tgcccatagt
     1741 gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat atgatcggaa
     1801 tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag cgtcatggcg
     1861 ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg atgtttggcg
     1921 atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg cgttcgccag
     1981 atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt aatctaaatg
     2041 ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc tctggcttgc
     2101 aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag atgaaaccat
     2161 gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc tgattgcagg
     2221 gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga tgggattagt
     2281 attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc taccatcgtg
     2341 cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg gatgacaaaa
     2401 cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga cttgaacccc
     2461 cacgtccgta aggacactaa cacctg
//
LOCUS       DAJTPG010000243         2483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTPG010000243 DAJTPG010000000
VERSION     DAJTPG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="OO230_004156"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="OO230_004156"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5693677.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..>2483
                     /gene="proA"
                     /locus_tag="OO230_004157"
     CDS             1283..>2483
                     /gene="proA"
                     /locus_tag="OO230_004157"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5693678.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLE"
BASE COUNT          587 a          631 c          721 g          544 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aag
//
LOCUS       DAJTPG010000244         2318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTPG010000244 DAJTPG010000000
VERSION     DAJTPG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..153
                     /locus_tag="OO230_004158"
     CDS             <1..153
                     /locus_tag="OO230_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5693679.1"
                     /translation="SSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIR
                     IQCLGG"
     gene            complement(242..754)
                     /locus_tag="OO230_004159"
     CDS             complement(242..754)
                     /locus_tag="OO230_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5693680.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            949..1713
                     /locus_tag="OO230_004160"
     CDS             949..1713
                     /locus_tag="OO230_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693681.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1664..>2318
                     /locus_tag="OO230_004161"
     CDS             1664..>2318
                     /locus_tag="OO230_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5693682.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     "
BASE COUNT          657 a          514 c          585 g          562 t
ORIGIN      
        1 tccagcgcct ccggcatggg caatgatgcc accgtcgaac tctctccagt tgctggacga
       61 aacgttctcg gtatccggga cggaatcatc agcgaccaga cagcattgag aatgcttcag
      121 gagtacatca ggatccaatg ccttgggggg tagcggtaat tttactcatt atccttcaaa
      181 tcagattctg ttgtcagagg aatgggggaa gctggattcg caagagcttt ttatgggcag
      241 tttagctaaa aaaaggcaac acgaagctaa aactaccaac ataaagtgct gaccagctag
      301 cagctgaaat cagactcact gtatacactt tcactggatg aagttgcaac attcctgcca
      361 ccaaaggagc aatgtaacgc agcacggcga taaagcgcga agtgaaaagt attgacacag
      421 agttattttg caactgaagg cgaacccgtt ctattgtatt ggaacggctc gatattatac
      481 atgcaagttg agggatatgc ccaattatta taccaagatg gtaattaacg atagtaccgc
      541 accatgcgcc actcatgacc gtgattccag cttcccatgg tgaaagagtc gtcttgctga
      601 ctgtaattac agtggccatc ataattgatg ctggtggcaa tatcgccgaa atgagtaaag
      661 tggattttgt gaacgctatt ataaaaagaa gcccccatag gcgatatgga tgaagtacaa
      721 aatagttcat caaagcattt atccactcca tcaatgtctt tccttctgtg aatttttctt
      781 tgctatttaa ccgtgatacg acttaattta ggcttaaaca ccttacattt acaaggtact
      841 cctgtggggg gccctgccac ggagcgtcgc aggagtgagt gatgaggaag aattaaagca
      901 acctattgtg acaggcagcg ataacagtaa atcacaggaa gcataatcat ggcaaaaccg
      961 gactgggagg ccatcgaatc ggcataccgg gccggagtcc ttagcctccg tgatataggc
     1021 gagaaatacg gcgttacaga aggggctatc aggaagaggg ccaaaaagct tgattgggca
     1081 cgcagtggcg gtacgcaggt ttgcaaaaat ggtacgcaaa aaaggaaagt gcgtaccagc
     1141 agaaagcctg ccattactgg ccttacacaa aaaagtacgc aactaaaaac agaatctaca
     1201 ccggatacga aaccgatacg cggaatgcgt accgatcccc cgactaaccc attccaaccc
     1261 ggtaaccagc aggcattaaa acacggtggt tatgcccgtc gccttctgct caaagatgag
     1321 gtgatagagg acgctaaagc gttgacgctc gaggacgaat tatttcgcct tcgtgctaac
     1381 aaccttgtcg ctgcagagaa tattggtcgg tggttggtgt cgctggaaga tgctaatggg
     1441 gaccaggaaa ggaagatgct gattgaaaat atcagcgctg ccgagaaagc aatgatgcgc
     1501 aatacagttc gtattgagtc catcgttggc acgcttgcga cggtaggaaa aatatttgct
     1561 gatacagcct accgcaaggc cgccactgat aaggtgtctc tggaggctga tcgtcttcgc
     1621 cgtgatgcag gtattgatga tggcaatgga gagcgtgacc tcaatgactt ctactctgac
     1681 atccaaaccg acgctgaatc cggctttacg tagtttctgg actatgcggg cacgtaacaa
     1741 agtgctttat ggtggtcggt catcgtcaaa atcatgggat gccgctggca ttgccatatt
     1801 tctgtcgaat aaatacaccc tgcgtttttg ttgtgcccgt cagatccaga ataaaatcga
     1861 agagtcggtg tataccctgc tcaaaattca gatagacagg tttggtctgc ggcaccgttt
     1921 ccgtattctg aacaacaaaa tcattaaccg ggttactggc tcggaatttg ttttttatgg
     1981 attatggcgc aacatcgaag aaattaagtc actggagggg atcgatatgt tgtggctgga
     2041 agaagcccac gcactgacgg aataccagtg gaaaattctg gagccaacga tccgtaaaga
     2101 gggttcggaa tgctggttca tattcaaccc cggacttgtt actgatttcg tctggcgcaa
     2161 cttcgttgtt gatccgcccg aaggcactct catccgcaaa attaactatg acgaaaatcc
     2221 gtttctgtct gacaccatgc ttaaggttat cgacgcggcg cgacgccgtg atccggatgg
     2281 ttttaaacat gtgtatgagg gcgttccgga gtctgatg
//
LOCUS       DAJTPG010000245         2227 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTPG010000245 DAJTPG010000000
VERSION     DAJTPG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(59..778)
                     /gene="yehT"
                     /locus_tag="OO230_004162"
     CDS             complement(59..778)
                     /gene="yehT"
                     /locus_tag="OO230_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5693683.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(775..>2227)
                     /gene="btsS"
                     /locus_tag="OO230_004163"
     CDS             complement(775..>2227)
                     /gene="btsS"
                     /locus_tag="OO230_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5693684.1"
                     /translation="GGLLGGPVVGGLVGLTGGLHRYSMGGMTALSCMISTIVEGLLGG
                     LVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRLVSNIAAPMM
                     VTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNEVNSMKVAQV
                     LYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEVVYADGNEVP
                     YRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNRLFSSINRTLGEGIAQLLSAQ
                     ILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQASQLVQYLSTF
                     FRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQQLPAFTLQP
                     IVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNASGLGMNLVDKR
                     LRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          545 a          608 c          520 g          554 t
ORIGIN      
        1 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
       61 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      121 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      181 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      241 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      301 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      361 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      421 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      481 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      541 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      601 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      661 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
      721 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
      781 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
      841 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
      901 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
      961 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1021 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1081 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1141 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1201 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1261 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1321 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1381 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1441 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1501 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1561 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1621 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1681 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     1741 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     1801 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     1861 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     1921 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     1981 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2041 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2101 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2161 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2221 gccgccc
//
LOCUS       DAJTPG010000246         2197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTPG010000246 DAJTPG010000000
VERSION     DAJTPG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(131..397)
                     /gene="ospE2"
                     /locus_tag="OO230_004164"
     CDS             complement(131..397)
                     /gene="ospE2"
                     /locus_tag="OO230_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HCR5693685.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(579..980)
                     /locus_tag="OO230_004165"
                     /pseudo
     CDS             complement(579..980)
                     /locus_tag="OO230_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1409..2197
                     /gene="virF"
                     /locus_tag="OO230_004166"
     CDS             1409..2197
                     /gene="virF"
                     /locus_tag="OO230_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5693686.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          676 a          389 c          407 g          725 t
ORIGIN      
        1 cgaggataga tggagttact cctctctgaa atagaattac tgctctactt tttctgtttc
       61 cagaaattat tagtaacaaa ccctgatata ttcttttgaa aatggagctt aaacgagtct
      121 gaaattaact tcagaaatca gaataccgtt ggctttgttt ttcatcaatt ccggcttcat
      181 ttatctttgt caaccattgc atttctgtag cagtgaattt caacgttttc gtccggttgg
      241 cagcaggtat ttgtaattcg tatatagctg cttttttatt gaaaacagta tatccatctg
      301 tagtaacagt agaagataat aatacaggat ttgatacttc aaggatgata ttttcttgtt
      361 tttgtggaag gcaagggaat atagtttgtg taagcatatt gcttccttat gttatatttt
      421 agtaaagaat ttatttttta cattatagtc ggctaaatga agtttactgt aaaaaaacga
      481 tacttttttc gtaagcgatg agatgtggag ccagaaaaat ggctgcgtta cgtcattgaa
      541 ctgtccttga cccacgatat tcagacagtc gttgtatctt aagttgacat tacccgctga
      601 cgtcgatatt cccttggtga gagataaccc aacgcactat gcggatggtt ttcattgtaa
      661 tgctcgatcg ccactgcaag gttatgcaat gccgttcttg gtttcggcat gaacgcgatg
      721 cagtcttcct tcatcgtttt catgaaccgt tctgctattc cattactctg agggctactg
      781 actgccgtcg tgcagggctc cagattcaac tctctggcga actgccgtgt ttcatgagcc
      841 gtataagccg aaccgttatc ggccactgga ttggccgttc cggtccttta tcgccatatc
      901 gctcctgaca tgacatcctg caccgttgct ttgtcataat ctcccgtgcc tgctgcccag
      961 tctatggctt cacgatcgca atatggaacg taacgctgtc atcgggagaa tcgtcaatgc
     1021 ggcattcggt taacgcttac caatatcaac accaaccgga tttgtggagc ctccagtctg
     1081 aaggggcttt atgcgttccg tataggatat tatgatgctg gagttttgcg aagctccact
     1141 cctttccgaa catttacaca gatattgcct aagaaaagtg aaaccataat atggttatag
     1201 tccctttcag tgcaaatact tagcttgttg cacagagaaa tagaagctgc ataagctctt
     1261 tcttcaaaaa atgtaaataa agttaaatat aggaaaaatt acttaatcta tcttaataac
     1321 ggaaagattt tgtatacaat cactgttaca gaaatttctt agttactctg taaacactaa
     1381 atatagtttg gtatattctg ttgaatttat gatggatatg ggacataaaa acaaaataga
     1441 tataaaggtt cgcttgcata actatattat tttatatgca aaaaggtgtt caatgacggt
     1501 tagctcaggc aatgaaactt tgactatcga tgaagggcaa attgctttta tagagcgaaa
     1561 tatacaaata aacgtctcca taaaaaaatc tgatagcatt aatccatttg agattataag
     1621 ccttgacaga aatttattat taagcattat tagaataatg gaaccaattt attcatttca
     1681 acactcctat tctgaggaga aaagggggtt aaacaaaaaa atattcctcc tctctgagga
     1741 ggaggtttct atcgatttgt tcaaatctat aaaagagatg cctttcggca aaagaaagat
     1801 ctatagttta gcttgccttt tatcagctgt ttctgatgag gaagctttat atacttcgat
     1861 atcgatagct tcttctctta gtttttctga tcagataagg aagattgttg aaaaaaacat
     1921 cgagaagaga tggcgtcttt ctgatatttc aaataacttg aatttatcag aaatagctgt
     1981 tagaaaacga ttggagagtg aaaaattaac atttcaacaa atccttcttg atattcgcat
     2041 gcatcatgca gcaaagcttt tattgaatag tcaaagctat attaatgatg tatcaagact
     2101 tatcggaata tcaagcccat cttattttat aaggaaattt aatgaatatt atggtataac
     2161 tccaaagaaa ttttacttat atcataaaaa attttaa
//
LOCUS       DAJTPG010000247         2112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTPG010000247 DAJTPG010000000
VERSION     DAJTPG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            271..575
                     /locus_tag="OO230_004167"
                     /pseudo
     CDS             271..575
                     /locus_tag="OO230_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            744..2108
                     /locus_tag="OO230_004168"
     CDS             744..2108
                     /locus_tag="OO230_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5693687.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          548 a          417 c          483 g          664 t
ORIGIN      
        1 ttggggagca atggaacagt aaacgccgtt aagaagtcaa tttaaatagc caaaaattaa
       61 ctagccaatt tacattgcag gttgtaattt ttaaccacct tttttacgat agccaaatta
      121 cctataccca tcccggccct gccaagccat acccgctatc ggcgatgaaa atgcttttgt
      181 gcgcggtcgg agtggtcccg acgagggttt tacccgaagt cggggcgtat ctccgcgtta
      241 gcgggccgtg agggccgctt acgagcgtgt atgagagacg tacagtggtt ttgtccgctc
      301 acaccagtac atacaggtac ggaaataaaa ccagtcgaaa tgccggtaac gacgattact
      361 cctgtctctg atgccggtgg actacaagat tttatttact ggcgtcctga tgcggacagg
      421 gctggtatgg aacctgttta tgtgatactt aatgatcctc tggattccgg gcgatttacg
      481 cgtaaacaac taaataaaaa tatcttaaac atgcaagtaa ttttggtatt ctcgatacaa
      541 gaataaatag tagtaactac tatagctgca aataatgtta aaaacatttc tatgatcatc
      601 tttacatttt tcatgattga caattgtttt tggttaaaaa aagacaacta atgagtgtga
      661 taatgcaata ctttgagtga ttatataatg ataagtaaac aggtttgcga caattggtta
      721 tcagtattac agaaggatta aatatgtcat tattcacaga acctaaggaa attgaaagat
      781 taccctctga agagattgaa cgattatatc ccgtcctgcg atacagggtc tttatcagca
      841 ttttcctcgg atatatgggg tattattttg tccgaaacac caccagtgtg ttatccggtg
      901 tgctgcacat gtctgcaacc gagatcggca ttatctcctg tgccggtttt ttatcctatg
      961 gtatatcaaa gtttgtttcc ggacttattt ctgacagaag taattcaaaa gtatttttgt
     1021 ctcttggttt attcctgtcc ggactggtaa attttctgat aggatatatt cccgggatca
     1081 tcacatccgt tacgttattt tcaacaatgt atttacttaa cggctggatc caaggtatgg
     1141 gatatcctcc tggtgccaaa acactggtgt tctggtatga acatcgggaa aggatcacct
     1201 gggccacact gtggaattta tcgcacaatg tcgggggagc tctggccccc gttctgattg
     1261 gtttcagttt tggttttttc ggcgacagtg ctctggatca tgcccgggcc gcatttatct
     1321 tcccaggagt actgtgtatg gcaatgagtg tgctgattta ctttattcag gtggacagac
     1381 ctgtatcagt ggggcttcct cccattgaag aatggaaagg taacgtcgtt tcacacccgg
     1441 ctaaaggccg tgaacaaggt ccgagactct ccattcctga tatcatcaga aaacatatca
     1501 tccgaaataa taaactcatt tactgctgta tctatggttc gtttgtttat attctgagat
     1561 acggtattgt ttcctgggct cctaagtttt taagtgacag tctggatgtc ggtggtaagg
     1621 atatgggaaa actggcatca atgggggggg gttctgtttt tgaaatcggt ggtgtggctg
     1681 gtatgcttct tgccggatac ctgtctgtca gactgttcag aaactcaaaa ccattaacaa
     1741 atactttatt tctggcactt accattattt tattaatcgc gtactggtac gtgccatcag
     1801 gcaatgaata tctctggcta aactatacaa tccttattct gttggggctg gcagtgtatg
     1861 gtccggtgat gtttattggt ctttattcaa tggagcttgt tcctaaagaa gctgccggag
     1921 ccgcatcagg gctgtcaggc acattttcct atatcttcgg ctcaattgtt gcgacactgg
     1981 gaatgggact tgttgtcgat tatctgggat ggggggccac atttatcgta ctgatactgt
     2041 ctgctgtttt tgccatcatt ttcacactga tgagtcgtga acgctcactg gagttcgaaa
     2101 aagagtaatt tt
//
LOCUS       DAJTPG010000248         2109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTPG010000248 DAJTPG010000000
VERSION     DAJTPG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..234
                     /locus_tag="OO230_004169"
                     /pseudo
     CDS             1..234
                     /locus_tag="OO230_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OO230_004170"
     CDS             655..1233
                     /locus_tag="OO230_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693688.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="OO230_004171"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="OO230_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..2109
                     /locus_tag="OO230_004172"
                     /pseudo
     CDS             1584..2109
                     /locus_tag="OO230_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          679 a          385 c          521 g          524 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatga catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag cagaccatgg agcgaggaaa gacgcaacga ctggcagaga tggctgcggc
     1921 caacagtgac gtccccatga tgaagaatgt ggctaaaacg atagggaaaa ggttgtacgg
     1981 tatcctgaac gccatgcgcc atggcgtatc aaacggaaat gcggaagcac tgaacagcaa
     2041 gatcaggctg ctgagaataa aagccaaggg ctaccgcaac cgggagcgct ttaagctggg
     2101 agtgatgtt
//
LOCUS       DAJTPG010000249         2092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTPG010000249 DAJTPG010000000
VERSION     DAJTPG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..799
                     /locus_tag="OO230_004173"
                     /pseudo
     CDS             1..799
                     /locus_tag="OO230_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OO230_004174"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OO230_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="OO230_004175"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="OO230_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          506 c          632 g          438 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc ctggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc tt
//
LOCUS       DAJTPG010000250         2086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTPG010000250 DAJTPG010000000
VERSION     DAJTPG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..352)
                     /locus_tag="OO230_004176"
     CDS             complement(<1..352)
                     /locus_tag="OO230_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5693689.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDAT"
     gene            complement(349..882)
                     /locus_tag="OO230_004177"
     CDS             complement(349..882)
                     /locus_tag="OO230_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5693690.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(948..1577)
                     /locus_tag="OO230_004178"
     CDS             complement(948..1577)
                     /locus_tag="OO230_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693691.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1581..1796)
                     /locus_tag="OO230_004179"
     CDS             complement(1581..1796)
                     /locus_tag="OO230_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5693692.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          533 a          555 c          457 g          541 t
ORIGIN      
        1 cggtggcatc attgcccatg ccggaggcgc tggaggtttc ggctgaggat ggcacagggc
       61 attttccttt gacgagcacc cgaccaccat tatcaagctt gcgccgaaga gcatcatttt
      121 cagctttcgc atcagctaac tccttcgtgt atttagcatc gagtgcatca gcatcacgct
      181 ggcgctgctg catgtcagta atggttgcgt tcgccagctt cagttctccg gcgtttttgt
      241 cgcgctgctc tttgtaggta atggcgttat cacggtaatg attaacagcc catgacaggc
      301 agacgatgat gcagataacc agagcggaga taatcgcggt gactctgctc attgatctat
      361 cccccaacag gctaatgcgc tttcctggtc acgacgaata acctgtccat agcagttatt
      421 tgaacgtatg cggcaatcgc gcccaacatc ttttatccac cagcgaatcg cctcgcatgc
      481 gcccttacga tcaccggcat tcagccgctt ataaaacgtc gacgggaaac acttaccggg
      541 gccaatgtta taggggcaaa atgacgctat acccgctttc tggggttcgg tcagaggtac
      601 tttaatattg cgctccaccc atgccagcgc cttatcacgt tcaatagcgt taacctggtc
      661 acatttttcc ttcgacagct tcattcccgg tatgacaggc ttaccatcca ccattgtggc
      721 accacgacag atggtccata tgccagaacc atcacggtat gccgttgtgt gattaccctc
      781 tttttcgttc agaaactggt caagtatctg gggagcagac gcgcctgcgg caatcagcgc
      841 cagaacagca gccgacaggc cgtatttgat ttttgcgctc atggatattt atcaggattt
      901 atcggcaaca aataacgagc caacttatat acgcctttta agataagtca gccccggctg
      961 gatccagtca tcaggctctt tcttaaaggg ggagtattga aatcacgaag aagagcctcc
     1021 cgcaccgcag catccatgtc tgcaccactg gcaagtgctt caatctctgc cgccacctgc
     1081 aaatacccca tgcaacggcc aatgcgtttc ataactctgg tgttttcgtc tttcaggcat
     1141 ctgatagcga gattaataat tttcaggcag tgatgatgcg atgcccggac aggttttccg
     1201 ttctcataaa cgacgaactc accctcttca caggaaaccc tgaaaggccg atggggttcc
     1261 tggtctttat ccggtgcaga cgcaataaaa tcacttttat gctcttcctc tccggcactg
     1321 ttaaccgtaa tgacgtactc aggccgcagg taagccgcct ctttttctga caatgcggcc
     1381 ggtaaaaact tttcctgcaa aaacttcata aaagtctcgt gagcgacttt gtggcagtaa
     1441 tcgtaaatcg ccgcataatg ctcatcgcgg cgtttgtttt catcttcaga actcaccaac
     1501 gccgacaatt ttttattaag ctcagtgatt tcattttcca ggtgattgaa tcgctgattc
     1561 atttcttcat gcttcattat ttactctccc cgtgcagcct tacgcttgtc ttctcttatt
     1621 ttgaaataca gattcgtcag ataagtcaga agccccagaa gcagacttcc cagcacacca
     1681 atcgcagccc actgtgatgg cctgacctga tccaaccact gcaaaaacca gtagccggca
     1741 ctgccggcgg aggtgccgta ggcaatgccc gttgaaattt tgtccatgga tttcatagcc
     1801 tcacctccgc aaataacgga tggcgtagtt ttacactgag aaatgaaagg gatttgaaaa
     1861 gaaaaaaacg caaaagcggg cgaaacgata tatacagtaa ggaaagcact ctatccaaca
     1921 aaccacccac agttaatcgg aataaaagca gagtgcttat gaatgatcgc acgcccgaag
     1981 gttagtattt ctgcacagca attttgcaaa aaaaggcgat cattcataac ttaaacgtct
     2041 ttcagtcact ccgggatttc ccatcatcgc agactgaaag actctg
//
LOCUS       DAJTPG010000251         2049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTPG010000251 DAJTPG010000000
VERSION     DAJTPG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..366)
                     /gene="arsC"
                     /locus_tag="OO230_004180"
     CDS             complement(<1..366)
                     /gene="arsC"
                     /locus_tag="OO230_004180"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5693693.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKG"
     gene            complement(379..1668)
                     /gene="arsB"
                     /locus_tag="OO230_004181"
     CDS             complement(379..1668)
                     /gene="arsB"
                     /locus_tag="OO230_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5693694.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1722..>2049)
                     /gene="arsR"
                     /locus_tag="OO230_004182"
     CDS             complement(1722..>2049)
                     /gene="arsR"
                     /locus_tag="OO230_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5693695.1"
                     /translation="FKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRHLAL
                     LRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNCSGD
                     SKNICS"
BASE COUNT          549 a          567 c          517 g          416 t
ORIGIN      
        1 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
       61 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      121 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      181 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      241 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      301 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      361 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      421 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      481 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
      541 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
      601 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      661 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      721 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      781 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
      841 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
      901 tgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
      961 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1021 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1081 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1141 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     1201 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1261 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1321 aagcgccgcg ccatcattgg caaacagggc cgcaacggcg gcaccgagca ggacaatcca
     1381 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
     1441 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     1501 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
     1561 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1621 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     1681 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
     1741 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
     1801 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
     1861 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     1921 gccagatggc gggatatctt gggctgcgac tgatcgaggg cagtgcagag atcgcagacg
     1981 cataactctc ccagttcgct aagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2041 attttgaac
//
LOCUS       DAJTPG010000252         2025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTPG010000252 DAJTPG010000000
VERSION     DAJTPG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..353)
                     /locus_tag="OO230_004183"
     CDS             complement(<1..353)
                     /locus_tag="OO230_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5693696.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMR"
     gene            complement(420..1634)
                     /locus_tag="OO230_004184"
     CDS             complement(420..1634)
                     /locus_tag="OO230_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5693697.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          629 a          490 c          412 g          494 t
ORIGIN      
        1 cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg cattaatgac
       61 cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag caaaataccg
      121 gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac accaaactct
      181 gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa tgacgaacgg
      241 tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt ctgaatacga
      301 cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc catttttagc
      361 tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc gaaccgttat
      421 taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc cgggtcatac
      481 ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc atcgcctggg
      541 tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat ttgttcggaa
      601 tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg cagcgccgcc
      661 gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac ctgataaagc
      721 gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt gatcgtcgtg
      781 cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat actgttaaac
      841 acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa acattcctgg
      901 ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat gctcttccca
      961 aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag atgttcgtta
     1021 aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag atacttttct
     1081 gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata cgccagtcct
     1141 ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg cacacgaatc
     1201 gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc aatcgtacgc
     1261 ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag cagttgatac
     1321 aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat ggcagaaatg
     1381 gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa ttgccagata
     1441 tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc tttcagataa
     1501 gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca aagtccgctt
     1561 tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa aacatctgca
     1621 gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa acctaatttt
     1681 acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac attaaaaaca
     1741 aaaatttcta ccgaggagaa aaaataccct caaaaaccac ctgaataatt aaccattatt
     1801 gttaaaacaa cacgttgaat aatcacataa caaactccat taatcattga aaatgagcac
     1861 agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat gtatttaaaa
     1921 atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa ggaatgtgac
     1981 aaataatggt tctggttggg cgttaaattt aaggtcacca gaatg
//
LOCUS       DAJTPG010000253         1996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTPG010000253 DAJTPG010000000
VERSION     DAJTPG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..787)
                     /locus_tag="OO230_004185"
     CDS             complement(<1..787)
                     /locus_tag="OO230_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5693698.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSL"
     gene            complement(1386..1634)
                     /locus_tag="OO230_004186"
     CDS             complement(1386..1634)
                     /locus_tag="OO230_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5693699.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1862..1927)
                     /locus_tag="OO230_004187"
                     /pseudo
     CDS             complement(1862..1927)
                     /locus_tag="OO230_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT          522 a          359 c          466 g          649 t
ORIGIN      
        1 gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt gaacattcat
       61 tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca aaataaccgt
      121 tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt gcccatatgg
      181 agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt gcgctaatct
      241 cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat acatctaatg
      301 tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag acatgaagct
      361 ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac gcattcaata
      421 atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg acattaagtt
      481 gtgtaaggga cgcaggcaat ggggagatta agcttaaatt atttttactt atattaattg
      541 atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac aagtccaggt
      601 tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct cgttgttctt
      661 cttgtatgcg gttatttttc cattcagtcc aatgggttag ataattttca tatgcgcggc
      721 tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg ttatttgtcg
      781 ggagcataaa tatcagggtg tattctttat gttgagaggt gttggattct tttttatctg
      841 ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg aggcggatat
      901 atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc tgctttacac
      961 tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg gggcaatttt
     1021 tccttaaggt atgaaaaaag gagctgaagc tatataatag ctattaatgc cactggtatc
     1081 agtgaatcat gtatataaaa aactgtgtta aggaggctgt ccattagcgg ttttttattc
     1141 ttctggaacc gcggcatctt tgcgtatctt tttgtcttct caccgtctgg tcggtgtttt
     1201 gctgagactg ctaatttcct gtctttgcta gtgctgtact ggtactgccc aatcatgatt
     1261 ggcgggggcg ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt gctgcggctg
     1321 gctggtgaac ttccgatagt gcgagtattg aatgatttcc agccgttact gattttacgt
     1381 gctaattagt gaacaaacca ctcgtcagca gactcccagg tatctttcag agtttcctga
     1441 acaaaagttt tagctgaatc tttatcggcg gtgcgcgtaa cagaaaggcc atcgttgctg
     1501 gtggctttta cgatcacctc tacatcgtca taacgtttac tgatgcgtcg ggttaattct
     1561 tcctttaacg catccacagc accgtttggc attttagtga ttttttcttt agcgatacag
     1621 atctcaacac gcataacacc ctcctgtaac tgtagttatg tacaggtgtt atttttatct
     1681 gtatggataa ccagtgtcaa ttcctgatat tgtttatggg gcatcaatgg ggcatgtatg
     1741 ggacactttt tatcggcgaa attcgtcgaa gttcgtcgac atggtaaacg aatcatctat
     1801 ccaacccttg aaaaacggcg ctcctggacg atcttcgtcg atttttaaaa atgttgcgtc
     1861 acgcgcgtaa cgtgacagga ttaatatcac aaagcaacgc cacttcacca attgtgtaaa
     1921 gcgccatcgt ctcacccttg ctcgcgaggt cccagtttaa ctttagacgc agttttgcga
     1981 accaggtagt tttgcc
//
LOCUS       DAJTPG010000254         1979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTPG010000254 DAJTPG010000000
VERSION     DAJTPG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            44..1979
                     /gene="sat"
                     /locus_tag="OO230_004188"
                     /pseudo
     CDS             44..1979
                     /gene="sat"
                     /locus_tag="OO230_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          729 a          342 c          390 g          518 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tactcatacg gcatgatgat
       61 ttataaaaat gataaaacat ttcgtaatct tgaaatattc ggagacagcg gctctggagc
      121 atacttatat gataacaaac tagaaaaatg ggtattagtc ggaacaaccc atggtattgc
      181 cagcgttaat ggtgaccaac tgacatggat aacaaaatac aatgataaac tggttagtga
      241 gttaaaagat acctatagtc ataaaataaa tctgaatggc aataatgtaa ccattaaaaa
      301 cacagatata acattacacc aaaacaatgc agataccact ggtactcaag aaaaaataac
      361 taaagacaaa gatattgtgt tcacaaatgg gggaaatgtt ctgtttaagg ataatttgga
      421 ttttggttgc ggtggtatta tctttgacga aggccatgaa tataacataa acggtcagag
      481 atttacattt aaaggagcag gaattgatat cggaaaagaa agcattgtaa actggaatgc
      541 attgtattcc agtgatgatg ttttacacaa aataggcccc ggtactctga atgttcaaaa
      601 aaaacagggg gcaaatataa agataggtga aggaaatgtt attcttaatg aagaaggaac
      661 atttaacaat atataccttg caagcggaaa tggtaaggta atactaaata aagataattc
      721 ccttggcaat gatcaatatg cggggatatt ttttactaaa cgtggtggca cgctagattt
      781 aaatggacac aatcagactt ttactagaat tgccgccact gacgatggaa caacaataac
      841 taactcagat acaaagaaag aagccgttct ggcaatcaat aacgaagact cctacatata
      901 tcatgggaac ataaatggca atataaaact aacgcacaat attaattctc aggataagaa
      961 aactaatgca aaattaattc tggatggtag tgtcaacaca aaaaatgatg ttgaagtcag
     1021 taatgccagt cttaccatgt aaggccatgc aacagagcat gcaatattca gaagcacagc
     1081 gagtcattgc tccctggtat ttctttgtgg aacggactgg gtcaccgttt tgaaagaaac
     1141 agagagttca tataataaaa agttcaattc tgattacaaa agtaataatc agcagacctc
     1201 atttgatcag cctgactgga aaaccggggt gtttaaattt gatacattac acctgaacaa
     1261 tgctgacttt tcaatatcac gcaatgccaa tgttgaagga aatatatcag caaataaatc
     1321 agctatcaca atcggcgata aaaatgctta cattgataat cttgcaggga aaaatattac
     1381 taataatggt tttgacttca aacaaactat cagtactaat ctatccatag gagaaactaa
     1441 atttacaggt ggcatcactg cacataacag ccaaatagcc ataggtgatc aagctgtagt
     1501 tacacttaat ggtgcaacct ttctgaataa tactcctata agtatagata aaggagcaaa
     1561 agttatagca caaaattcca tgttcacaac aaaaggtatt gatatctccg gtgaactgac
     1621 tatgatggga atccctgaac agaatagtaa aactgtaacg ccgggtctcc actacgctgc
     1681 tgatggattc aggttgagtg gtggaaatgc aaatttcatt gccagaaata tggcatctgt
     1741 caccggaaat atttatgctg atgatgcagc aaccattact ctgggacagc ctgaaactga
     1801 aacaccgact atatcgtctg cttatcaggc atgggcagag actcttttgt atggctttga
     1861 taccgcctat cgaggcgcaa taacagcccc caaagctaca gttagcatga ataatgcgat
     1921 ctggcatcta aatagccagt catcaattaa tcgtctagaa acaaaagaca gtatggtgc
//
LOCUS       DAJTPG010000255         1971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTPG010000255 DAJTPG010000000
VERSION     DAJTPG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(149..313)
                     /locus_tag="OO230_004189"
     CDS             complement(149..313)
                     /locus_tag="OO230_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693700.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1215..1499
                     /locus_tag="OO230_004190"
     CDS             1215..1499
                     /locus_tag="OO230_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5693701.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1487..>1971
                     /locus_tag="OO230_004191"
     CDS             1487..>1971
                     /locus_tag="OO230_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5693702.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          506 a          417 c          565 g          483 t
ORIGIN      
        1 taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc tgcaataccc
       61 cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag ctgatggagt
      121 tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg tttccggcgt
      181 aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta tgagagatga
      241 ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta agagcggtgg
      301 tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg cggagatgaa
      361 cgaaagtgaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg aaaatgtgct
      421 atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg gcatccggca
      481 tataggcagc atgaacgcaa cacaggctct cgtacggaac gtgggaacct gtcgtctcga
      541 tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga tgcgagacac
      601 aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga gcgaaggggg
      661 tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc atgagtcagc
      721 caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg gtcaaagcta
      781 atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt accaatcgaa
      841 gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg cccgcaaaca
      901 caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa ttcgtcacga
      961 ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg ttcaacgaca
     1021 agagccgtat gacgcgagag tgtcacgtac ggctccgtgg gagcgtaggg gggcggtgcc
     1081 cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa atgccgctga
     1141 cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt caatatgcta
     1201 ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag ctcaatctca
     1261 gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt cataagtcgc
     1321 gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt ctcgaccggc
     1381 gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt gatgctccgg
     1441 ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg gatgtgtaac
     1501 tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagca gtgaagctgt
     1561 acttgatgac tggttaaagc agaaggagct aaaaaatcag gcaatcggag ccacccggac
     1621 ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac tggccactgg
     1681 tagtgtcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag accccattcc
     1741 tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag ggcttggcgc
     1801 ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata tcggtgtgcg
     1861 tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc atcatggttt
     1921 taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat a
//
LOCUS       DAJTPG010000256         1953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTPG010000256 DAJTPG010000000
VERSION     DAJTPG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..353
                     /locus_tag="OO230_004192"
     CDS             <1..353
                     /locus_tag="OO230_004192"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5693703.1"
                     /translation="KGCPNYPPEFKQLLVAASCEPGISISKLALENGINANLLFKWRQ
                     QWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGNV
                     SEKLLTLLIQELKR"
     gene            350..697
                     /gene="tnpB"
                     /locus_tag="OO230_004193"
     CDS             350..697
                     /gene="tnpB"
                     /locus_tag="OO230_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5693704.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            747..>1953
                     /locus_tag="OO230_004194"
     CDS             747..>1953
                     /locus_tag="OO230_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HCR5693705.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLF"
BASE COUNT          444 a          551 c          565 g          393 t
ORIGIN      
        1 gaaaaggctg ccctaattat cctcccgaat ttaaacagct gctcgttgct gcctcctgtg
       61 aacccgggat atccatctca aaacttgctc ttgaaaatgg cattaacgcc aatctgttgt
      121 tcaaatggcg acaacaatgg cgcgagggaa agctgctatt accttcttca gagagccccc
      181 agctacttcc tgtgactctc gatgcagctg ccgaacagcc agaatcgctc gcagaggacc
      241 cggaaaccct cagtatcagc tgtgaggtaa cgttccggca cgggacgctc cgcttcaatg
      301 gcaatgtcag cgaaaagctc ctgactctgc tgatacagga actgaagcga tgatcccgtt
      361 accttccggg accaaaattt ggctggttgc cggtatcacc gatatgagaa atagcttcaa
      421 cggcctggct gcgaaagtac aaacggcgct gaaagacgat cccatgtccg gccatatttt
      481 cattttccgg ggccgcagcg gcagtcaggt taaactgctg tggtccaccg gcgacggact
      541 gtgcctcctg accaaacggc tggagcgtgg gcgcttcgcc tggccgtcag cccgtgatgg
      601 caaagtgttc cttacgcagg cgcagctggc gatgctgctg gaaggtatcg actggcgaca
      661 gcccaagcgg ctgctgacct ccctgaccat gctgtaaatc tctttatcct ggttgtcaca
      721 gaataagccc ggtaaaatac gggcttatga acgacatctc ttctgacgac atcttcctgc
      781 tgaaacagcg cctggccgaa caggaagcgc tgatccacgc cctgcaggaa aagctgagca
      841 accgggagcg cgaaatagac catctgcagg cgcagctgga taaactccgc cggatgaact
      901 tcggcagtcg ttccgaaaaa gtctcccgcc gtatcgcaca aatggaagcc gatctgaacc
      961 ggcttcagaa agagagcgat acgctgactg gtagggtgta tgacccggca gtacagcgtc
     1021 cgttgcgtca gacccgcacc cgtaagccgt tccctgaatc actaccccgt gacgaaaagc
     1081 gactgttgcc tgcggcgccg tgctgcccga actgcggcgg ttcactgagc tatctgggcg
     1141 aggataccgc cgaacagctg gagttgatgc gtagcgcctt ccgggttatc cggacggtac
     1201 gggaaaaaca tgcctgtact cagtgcgata ccatcgtgca ggcacctgca ccttcgcggc
     1261 ccatcgagcg gggtatcgcc ggaccggggc tgctggcccg cgtgctgacc tcgaagtatg
     1321 cagagcacac cccgctgtat tgccagtcag aaatatacgg ccggcaaggt gtggagctga
     1381 gccgttcact gctgtcgggc tgggtggatg catgctgccg gctgctgtct ccgctggaag
     1441 aggcgcttca tggctatgtc atgactgacg gcaaactcca tgccgatgat accccggtcc
     1501 aggtactgct gccgggtaat aagaagacga agaccgggcg gttgtgggcg tatgttcgtg
     1561 atgaccgcaa tgccgggtca gcgttggcac ctgcagtgtg gttcgcttac agcccggaca
     1621 gaaaaagcat ccatccgcag actcatcttg cttgcttcag cggtgtgctg caagcggatg
     1681 cgtacgccgg gttcaacgag ctgtatcgca atggtgggat aacggaagct gcctgctggg
     1741 ctcatgcccg ccgaaagatc cacgatgtgc acgtccgcat cccgtcagca ctgacggaag
     1801 aagccctgga gcagatcggt cagttgtacg ccatagaggc ggatataagg ggaatgccgg
     1861 cagagcagcg gcttgctgaa cgtcagcgaa aaacgaaacc gctgttgaaa tccctggaaa
     1921 gctggttgcg tgaaaagatg aagaccctgt tct
//
LOCUS       DAJTPG010000257         1953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTPG010000257 DAJTPG010000000
VERSION     DAJTPG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            96..290
                     /locus_tag="OO230_004195"
     CDS             96..290
                     /locus_tag="OO230_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693706.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(881..1024)
                     /locus_tag="OO230_004196"
     CDS             complement(881..1024)
                     /locus_tag="OO230_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693707.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            1263..>1953
                     /locus_tag="OO230_004197"
     CDS             1263..>1953
                     /locus_tag="OO230_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5693708.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEV"
BASE COUNT          625 a          412 c          404 g          512 t
ORIGIN      
        1 ttgacgacaa ttccaatgcg gataaaacac gtccaccaca ctgattacat ttttcattag
       61 cacagtggtg tgatgcagca tacgaggaaa accggatgaa tgagaacaaa ataaaaaggc
      121 tggagcaact gttacaggca cgccaacggg agtttgccac taaatgggga cgtgccgcgc
      181 tgccacatga acgcctaacc tattttccac ttgaagacct cattaaaaat aatggcaaaa
      241 aaatcaggtc tgataagaaa cacttcttag aatgcaatga agacaaataa caacaatctg
      301 catattgcca ccaaccacag atgtggcatc gttatcagcg cacttgattt tctttatatt
      361 cactgacgag cttgccgggg cacctttgtt tgccggtcat tctggtatca tgcagatatt
      421 tacggcgtac aagcccacta gcatgacatt gtaataaggc ctgagcctaa cgctttaatg
      481 tgtgtcatac actcagtggc gtaatcgttg acaatatggt gcatgtaatc gtaagcgtcc
      541 agcgaaatcc atctggtcat aatctgaagc atccgacaaa gtggtgtcca ccaaataagt
      601 gatctgcgcc caatatcctg agccggaact ttgacaaagg ccaacgaaaa atgggagcgg
      661 atgttacaga gttctcggta caggggaaaa gtggtacctg tcgccagttc tccgatctgt
      721 ttaaccggga aattatctcc tacagcttgt cagaaaggtc ggtgatggag catcgttaat
      781 acgatgctat acgatgcatt cacagtgccc ggccagagga tgccccgtcg ctgcatatgg
      841 atcagggttg gcaatatcga atcgcaggct ataggcaaag ttaaagcccc atggagtagc
      901 acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga acttcttcgg
      961 cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact gaacgatgtt
     1021 ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc ccacattggg
     1081 caaaaaaaaa caaaaaacga tcaattagta ttgattcaca taatctctca tcattaatga
     1141 gaacattgtc acatatttac aatatagaac tgttactttt agcaagctgt tagtaaccaa
     1201 ccgttactaa taattttaat tcttaatact ttgttcattc aataaatgag ttgagagaat
     1261 atatgaataa aatatactcc cttaaatata gtgctgccac tggcggactc attgctgttt
     1321 ctgaattagc gaaaagagtt tctggtaaaa caaaccgaaa acttgtagca acaatgttgt
     1381 ctctggctgt tgcaggtaca gtaaatgcag caaatattga tatatcaaat gtatgggcga
     1441 gagactatct tgatcttgca caaaataaag gtattttcca gcccggagca acagacgtaa
     1501 caatcacttt aaaaaacgga gataaattct ctttccataa tctctcaatt ccggattttt
     1561 ctggtgcagc agcgagtggc gcagctaccg caataggagg ttcttatagt gttactgttg
     1621 cacataacaa aaagaaccct caggccgcag aaactcaggt ttacgctcag tcttcttaca
     1681 aggttgttga cagaagaaat tccaatgatt ttgagattca gaggttaaat aaatttgttg
     1741 tggaaacagt aggtgccacc ccggcagaga ccaaccctac aacatattct gatgcattag
     1801 aacgctacgg tatagtcact tctgacggtt caaaaaaaat cataggtttt cgtgctggct
     1861 ctggaggaac atcatttatt aatggtgaat ccaaaatctc aacaaattca gcatatagcc
     1921 atgacctgtt aagtgctagt ctatttgagg tca
//
LOCUS       DAJTPG010000258         1941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTPG010000258 DAJTPG010000000
VERSION     DAJTPG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            29..1426
                     /gene="ccp"
                     /locus_tag="OO230_004198"
     CDS             29..1426
                     /gene="ccp"
                     /locus_tag="OO230_004198"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5693709.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1637..>1941
                     /locus_tag="OO230_004199"
     CDS             1637..>1941
                     /locus_tag="OO230_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5693710.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          538 a          400 c          490 g          513 t
ORIGIN      
        1 aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta ccgcgatcgg
       61 cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt accacgataa
      121 taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata ataaggtttt
      181 aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag aattacccgc
      241 ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc ttggatataa
      301 atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg tttcgcaaag
      361 cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa cgcgttatac
      421 cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac tggcctggat
      481 tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc atcgcaatga
      541 accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg cgttgggctt
      601 tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg ctcattgcca
      661 tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg gtggcgcagt
      721 tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc agttctggga
      781 tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc cgattgaaat
      841 ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc agcttaaagc
      901 gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg atgccattgc
      961 tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt tgcgtggaga
     1021 tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag ataataaatg
     1081 tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt tggggctgaa
     1141 aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga atgtgactaa
     1201 agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg ctttaacggc
     1261 accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac tgatgctgcg
     1321 ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag ctttcctgca
     1381 cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa tttgatcgcc
     1441 cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt aatattatca
     1501 tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt atttaaatta
     1561 agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc cttcaaataa
     1621 atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag aactactcga
     1681 ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat ttccgctgca
     1741 cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc ttgatggcaa
     1801 cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg tccataaatt
     1861 gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc aatccgcagc
     1921 catcgacctg cgttgcgtaa a
//
LOCUS       DAJTPG010000259         1910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTPG010000259 DAJTPG010000000
VERSION     DAJTPG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            353..1711
                     /locus_tag="OO230_004200"
     CDS             353..1711
                     /locus_tag="OO230_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5693711.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT          505 a          473 c          514 g          418 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa
//
LOCUS       DAJTPG010000260         1887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTPG010000260 DAJTPG010000000
VERSION     DAJTPG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(43..1692)
                     /locus_tag="OO230_004201"
     CDS             complement(43..1692)
                     /locus_tag="OO230_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5693712.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          444 a          499 c          485 g          459 t
ORIGIN      
        1 attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt cgccgtacaa
       61 accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca acggatactc
      121 gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga tcattttgtg
      181 ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt catcacccag
      241 aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg gtgcccagcc
      301 gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc cgccaggtgt
      361 cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt tcgccatgcc
      421 gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc gtcgccagtc
      481 gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg catcgcgacg
      541 ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca gcgccgagat
      601 gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg ggatgaactg
      661 ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag agtaatccca
      721 accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc gaccagaatg
      781 tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc gatcgtccca
      841 gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg cctgattagg
      901 gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt ggtcgagata
      961 gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg caaaaaccgg
     1021 tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac cgtagttttc
     1081 gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac cactttccgc
     1141 cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac ggccgcccgg
     1201 gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat cctgtggttc
     1261 gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt gcgggtccga
     1321 tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa acttgcgcaa
     1381 gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca gcgggtccat
     1441 tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg actgaacatg
     1501 ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg gcagcccttc
     1561 aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt catacgggtc
     1621 cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt tttgaatttt
     1681 ctgattgagc ataaggatcc agcctccgaa attcgtcgct aattatcggg cttttcttta
     1741 agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca cgtttattaa
     1801 cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg gatttggtgt
     1861 tgtttttcat tttcagaaca ttcaatc
//
LOCUS       DAJTPG010000261         1849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTPG010000261 DAJTPG010000000
VERSION     DAJTPG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(52..408)
                     /locus_tag="OO230_004202"
                     /pseudo
     CDS             complement(52..408)
                     /locus_tag="OO230_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            488..1681
                     /locus_tag="OO230_004203"
     CDS             488..1681
                     /locus_tag="OO230_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5693713.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1701..1849)
                     /locus_tag="OO230_004204"
                     /pseudo
     CDS             complement(1701..1849)
                     /locus_tag="OO230_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          499 a          420 c          458 g          472 t
ORIGIN      
        1 gttgcaactg cccggcgagc gccatcagtc gttgatgttg cagttccatc atcacgccac
       61 tcctctgcag aatgagtcgt agatggagag tggatgatgc tggggggggt tgtcgaagct
      121 caccagattt tcaccaggat gcacgtcata ctcttttttc tccggaggca gtgccagcat
      181 ggactgctgc tcttcgagcc agcgatcaca gggacgggcc tggattgttt catgctttcg
      241 ttggttagcg acatcgtgca gccagcgcag accgtggcgg ttggctgttt caacatcgac
      301 agtgctcccc atcgggcgca ggcgagtcat tagtgggatg taaaaactgt tacgggtgta
      361 ctgcaccatc cgttccacct tacctttagt ctgtgccctg aagggctgag gaatccccag
      421 aaaacgagac aaacatagca ttatgtgatc tactgatttg tgccaacaat tcaatgagat
      481 cactgctatg cctgttcata aagtatgtca gaatttcttc cgggatgcct tagcaccatt
      541 tcatcagtac cgacaaaatg ccctgatgga tgccacaatg gcgttaatca acggagcatc
      601 tctgacgcag accagtatcg gacgtttttt gccgggtaat gcacaagtta agaataagat
      661 caaacgtatt gatcgcctca tgggtaatga ggcgctccat cgcgatattc caatgatttt
      721 caggaatatc acttcaatgc tgacacggca gttatcttta tgtgtaatag ccgtagactg
      781 gagtggctat ccctcccagg agcatcatgt tcttcgtgcc agccttctgt gtgacgggcg
      841 ttccattccg ttattgagta aggttgttcc ttctgaaaaa caaaataatc cattgataca
      901 acatgatttc cttgattccc ttgctcaatc cctgcccccc gatgccaggg ttattattgt
      961 cacggatgct ggatttcaga gcgcatggtt tcaccatatt acttctctgg gctgggattt
     1021 tattggtcgg atcagaaata acgttcagta ttgcctcgat aacgcgcctg aaagatggtt
     1081 aaaagtgtcg gatagccctg aatgtaaaac accggaatat atgggggctg gaaggctggt
     1141 taaagaaagg aaaaaaagca tccgggggca tttttacacc tataagaaat cggcaaaagg
     1201 gcgaaaaaag aaacgttcga aaggtcagag tggtttaaac aaaacagaca aagaacagag
     1261 caaatcagca aaagaagcct ggttgatctt cagcagtaca aatgacttca gagcgcgaga
     1321 aatcatcaag ttatacagtc ggcgaatgca gatagagcaa aactttcgtg atgaaaagaa
     1381 cgggcgcttc ggatttggtc ttcgggccag caaaagtcgt tcaacaggaa gaattctggt
     1441 tctgagtctg ctggcaacgt tgagcactat cgtaatgtgg ttactcggtt atcacgctga
     1501 aaataaaggg ttacacctga aatatcaggc taacagcatt aaatcccggc gggttatctc
     1561 atatctgacg ttagcgaaga atgttcttcg acactcgcca cttattttaa gacgaacagt
     1621 actgagcaca gttctaaacc atctgtccag aacctaccga aatatggtgc tggtttatta
     1681 gcagtaattt tgtggggatc cctcagcctg aaggggcgac acagtcgggg agagaagccc
     1741 atctccttgc cgaactgcca cagcgaagga tggaaccggt gctgaccggt ctgatatgcg
     1801 tcacgttgca gaaccacagt tttcatattg tcatacaaca cttcgcgcg
//
LOCUS       DAJTPG010000262         1812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTPG010000262 DAJTPG010000000
VERSION     DAJTPG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            109..293
                     /locus_tag="OO230_004205"
                     /pseudo
     CDS             109..293
                     /locus_tag="OO230_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            436..657
                     /locus_tag="OO230_004206"
                     /pseudo
     CDS             436..657
                     /locus_tag="OO230_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..740)
                     /locus_tag="OO230_004207"
                     /pseudo
     CDS             complement(678..740)
                     /locus_tag="OO230_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1190..>1812
                     /gene="ospC1"
                     /locus_tag="OO230_004208"
     CDS             1190..>1812
                     /gene="ospC1"
                     /locus_tag="OO230_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5693714.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGA"
BASE COUNT          626 a          362 c          329 g          495 t
ORIGIN      
        1 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
       61 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      121 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      181 gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      241 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      301 agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac
      361 tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg
      421 ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg
      481 ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag
      541 cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag
      601 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca
      661 ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg
      721 attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc
      781 caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      841 tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      901 atcagagaaa gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata
      961 gttattaaat tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga
     1021 ttttagatat tttgaaatat ctggcatcac ataacatcaa aagtatataa tactcttatc
     1081 taattacatg cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc
     1141 ttgtagggaa agatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc
     1201 agaaacactg aactcagcaa atacccaatg caatatagat tctatggata acagattaca
     1261 tacattgttt ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga
     1321 aaaaaattta aaagatagtg caaatattat taaaagtttc tttaggaaaa ctatagcagc
     1381 acagagttat agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat
     1441 tgatgcacca tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc
     1501 gaagcattat atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa
     1561 tttgctttcg ctaattatta attctgattt aatcttcaga catcaaagta attctgattt
     1621 gtctgataaa attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac
     1681 aaaacgaaac acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt
     1741 tggcgttgaa atctctaacc atcagaaaaa acaccccctg aatacaaaac atcacactgt
     1801 tgattttggt gc
//
LOCUS       DAJTPG010000263         1809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTPG010000263 DAJTPG010000000
VERSION     DAJTPG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            173..1198
                     /locus_tag="OO230_004209"
     CDS             173..1198
                     /locus_tag="OO230_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5693715.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="OO230_004210"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="OO230_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          420 a          422 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggcccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAJTPG010000264         1801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTPG010000264 DAJTPG010000000
VERSION     DAJTPG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(84..1688)
                     /locus_tag="OO230_004211"
     CDS             complement(84..1688)
                     /locus_tag="OO230_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5693716.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          446 a          456 c          410 g          489 t
ORIGIN      
        1 cccttgacaa gtagagaaaa ctatgagtca aaaacatgac cacacaggtc tgtagtaagt
       61 gtcatcctag actatggtct cctctaggtc tccttataaa catcagccat cacattgacc
      121 agcctatcaa cctcgacctc tttagcataa cggtcgtacg taatagaccc tgaactatgc
      181 cccatgatcg cctgtgcata cgccaactcg accccatgtt gttttaactg ggccgcaact
      241 gtatgtctaa acgagtgaaa cgctaagccg ctacggtcta cgaggacttt aggcaacaac
      301 gtccgattaa accatttact cacctgctca ccgtaaccgt tcttcatgag acgcagacca
      361 tcgaacacca tagcgtcatc accgtcagcg cctctacggg tctctacaag cgccataaag
      421 tcagccaata taaaaccata ggccccatcg accagaggca cacagcggtc actatgagcg
      481 ttcttgatgc tcttaccggg tagactacta tcgtcttcat tgatatgaat ataaacggtt
      541 cccgcctcag tggtcctgac gtctttgacc tgtagttggg ctatttcatt gagtctcgcc
      601 ccagtgatag cagccaaggc ggtaacataa taatgatacg gcttaccaga ggtcttctga
      661 gagtatgcct tagctgcgac cagtagttgc cccacctgct ccgtagagaa ggcgttacgg
      721 gcccctgagg ctttacgctg agggacttta agctccagac cctcagtcat gttcttttta
      781 atcaagtcat tgcgcacagc ccacctaaag acagcagcca tcttaataag atacttgttg
      841 ttgatggtaa cgacatccag acaatctgtc ttatcttcac ggctcaacag gtcagcaagt
      901 gggacatcct tgaagcgctg cttacggttc ctaggtaact gctggagcac atcacggacc
      961 ctaagcatgt cagccctgtt agcgtctgca ttgagaccca gatggtcgaa tatttcgatt
     1021 aacgcagcgt gtgacgcctt gttttccctt aaggttgcag gcttccagtt ctgatagttc
     1081 tccgcttcgt attgttcaaa gaggctcgct atggtaatgc taggcgtaac ttctggtttt
     1141 acttctggag cattaacaga tagggacaca gagtctgctt ggttatcttg cctatcaata
     1201 tcattaaaaa gatcgacgta atctatcaga ggctgactgt ctcccgcttc gatacgcctc
     1261 atacaggctt ttagcacctt cagggcctca ttgatgtaac gatgctggtc aatgcttagc
     1321 ggctcactag ccagagcatc ggtaaggctc tcaccaagtt ctccgtattg gtcccggtag
     1381 atatccctca tgtcaggctc aaagagatct gaacggccca tagacagttc acactcagcg
     1441 atgtccttaa ggtgttccct catttcctca taggtagcgt tgggtctgtc tgcgtgtatt
     1501 gccttgagtg ctgcctttat gtgccttgag taagccatag cggtgcgtct atccttggtc
     1561 cgtaatgata ttgaggtcat tctatcattg tttgattgtc tgaccctaag acggaagtag
     1621 tagacgccgt tacgctggta gagatagtag actgctggaa gtttgtgact actggatagc
     1681 tgaatcatat gtctgcatgc cctgagaggg ttacacagtg ggttacagta agtaactctg
     1741 caaggaggct taaagccgta tcccttagga ttcagtaggt tggagtactt taagaaaggt
     1801 c
//
LOCUS       DAJTPG010000265         1800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTPG010000265 DAJTPG010000000
VERSION     DAJTPG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..198)
                     /locus_tag="OO230_004212"
     CDS             complement(<1..198)
                     /locus_tag="OO230_004212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693717.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNK"
     gene            complement(951..1424)
                     /locus_tag="OO230_004213"
                     /pseudo
     CDS             complement(951..1424)
                     /locus_tag="OO230_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1352..1718)
                     /locus_tag="OO230_004214"
                     /pseudo
     CDS             complement(1352..1718)
                     /locus_tag="OO230_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          408 a          501 c          430 g          461 t
ORIGIN      
        1 tttgtttccg gcatccacaa agaacggaat attcaggtct gcgcaacact caacggcatc
       61 gtcaaaatta tcaaagcgca gaacctcagc ttcttcttcg tcaaaaaaat catcttcgtg
      121 aagttttacg acatagcgga ggagtttgct tctttgaggt gcaggttttc gtttgcgtgg
      181 tttagctgaa cgggccatat aactacctga aagacaatga tattgtctgt ttttataacg
      241 gtaatcacag gccatgacaa gccgcagccg tcaggccgcc tactcggggc ggtttagaac
      301 gcttatttag taccagatct tacttataat aaaggggctt ggcagtttgg ataaactatt
      361 gtccttattc cactaataca aactttttgt aaagtatatt aaatatactt catttcatac
      421 tgaaatcccc ggaagtattc aatcaaacaa ttacaccata aaaggaagag ggggccccaa
      481 ttaagttgtc aagcatgttt actgcgcaat cgcttggtgc tccgtccctg taccccgcat
      541 tggtactctg gcgctcatgg ggcttccact tgcgcttctc ccttcacatc ggggtgacag
      601 gtccacgttc cccacaagag cctgaatcga cttcacgccg cttttatgcc ggatgccgcg
      661 caggcagtaa acatatccca caggaacgac tccaccattg ttttgacatc atctatatgc
      721 tttcgacaca tcatcagcgg ttcactttcg ttcgtctctt cgaaccatac ctgatcggct
      781 cacgtccgac cttttcccat ctcgctcacc accacatctc ttaaatgcag cagcagtgga
      841 tggtttggtg cctgcgcctg cacgccgaca ccaaggggcc ttccctcatc tcttgtgctg
      901 catggctgca cagggactca cgtccccagg acgcgccttc gtggcacaca gtcatcggca
      961 tagcaacagt aagcaacggc aggtttccat tgccagtttt ctttgacagc agtacttcgg
     1021 cctcgctgga tactgtgatt ccagtaccag cgatcctttc tggcttttcc actcaggtag
     1081 cgtttattca gatactggtc gaactcattc agtatgatgt tcgacagcaa cggcgagatc
     1141 acaccgccct gtggcatact ttcactgacc gcccggaaga gaccaacatc aacacggcgt
     1201 tttccacagc agtctcataa aacgtgtatc actgatcctg cggcagacgg ctttcatcag
     1261 cagccggtga tgtactgtgt cgaagtagct ggacaggtca ccttcaataa cccggcgccc
     1321 cgtgtttccc cgcagtcggt gagctgtaat agtacgtgat gtctcccgcc cacttctggt
     1381 tcgggccact ggcgtaaaaa tcctgcgtca gcagattttc tgatacaggc aggccgtgtg
     1441 cacggtaact gatcgggctg aacttctggg aggcttttgc gctcagttcc tgacggcgca
     1501 gactggctgc cacggttttt acgttaaagt ggtaaccaga gcacacagtt catccgtcag
     1561 gcgtggggca ccgtaacgct ggactgggta aaagccttgc ggacaacgct gtcgcagtgc
     1621 tgacggaact gcggacgtgt gtttatcctt gtccgccgct gacaccacgt ataccagccg
     1681 ctgcgggcca cccggagtac gcggcacatt gccttgatga cggtttacgc ggtcgcctgc
     1741 ggcaacggct ttccggctga cggtagtgtg gaccttttta ccccggagaa caccggcaag
//
LOCUS       DAJTPG010000266         1731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTPG010000266 DAJTPG010000000
VERSION     DAJTPG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            193..462
                     /locus_tag="OO230_004215"
                     /pseudo
     CDS             193..462
                     /locus_tag="OO230_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            459..685
                     /locus_tag="OO230_004216"
                     /pseudo
     CDS             459..685
                     /locus_tag="OO230_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="OO230_004217"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="OO230_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5693718.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1643..1731
                     /locus_tag="OO230_004218"
                     /pseudo
     CDS             1643..1731
                     /locus_tag="OO230_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          500 a          360 c          411 g          460 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgactaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagacgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggaccatt ccctcgcgat attaacgtaa
     1381 tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga tcacggcagc agacaggtaa
     1441 aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt atgataaatt ttaaccacat
     1501 gcatacctgt gcaacaaggc gaatcacgca cggatttcca gccagagaca cctgaaacgt
     1561 aaccggctca tttaaaccgt ctggtctgtt acctccggtt ccacaaaaat aatgtccatc
     1621 attttgaagt ggacactatc gtatggaata ccggacctga atcactgaag ctttacgcct
     1681 tcatttcgaa gaacatttac cacgggttgt ggccggatgt cgcctgggcg t
//
LOCUS       DAJTPG010000267         1716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTPG010000267 DAJTPG010000000
VERSION     DAJTPG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..237
                     /locus_tag="OO230_004219"
     CDS             <1..237
                     /locus_tag="OO230_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693719.1"
                     /translation="SVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLT
                     VSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            239..844
                     /locus_tag="OO230_004220"
     CDS             239..844
                     /locus_tag="OO230_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5693720.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            831..>1716
                     /locus_tag="OO230_004221"
     CDS             831..>1716
                     /locus_tag="OO230_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5693721.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQYKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANS"
BASE COUNT          405 a          387 c          485 g          439 t
ORIGIN      
        1 tccgtgctgg acgcatttaa tggtacagat ggtggtctgc gggcaagagt tgcctctgtt
       61 gtctctgccg ggcgctacta tgccggcatt tacaaaaccg atccggaaca tattgatatt
      121 ctgggcctta ctgtgagccg tgatggttcg tcatggacaa cggctgtcac tttcgggata
      181 gatgagattc cggttctgga tgtgtcggat atcagtgtga aactccagga ggcataacgt
      241 gcagaatgtg gctgccactg tgcttgcgca gtatgctgcc agcccccgac tcaatgccct
      301 cattaacagc tttaacgcag cgctttcccc cgacagtttt atcagtgatt tttatggtct
      361 tatctggaac atcgataccg cagaaaagta tggtcttgat gtctggggaa agattgtggg
      421 tgtcagtcgc tggctgacgg taaaggacga ttttaattac ctgggcttca gcgagtccag
      481 gatggacacc ccggtaatgg atgatccctg tccgtttaat caggcaccgt tttacaacgg
      541 aaaatcggat acccggactg ttgacctgtc tgatgctgta taccggcggc tgatactgat
      601 gaaagccatg tcgaacatta ctgactgttc cgttccggat attaacctga tgctgagatt
      661 tatgttcgga aaaaaacgcc gggcttatgt tctgaataat ggcgggctga ggatgagtta
      721 cgtctttgag tccgcgctct cgttggcaga actggcgatt atccagtcgt cgggtgcact
      781 gccatccccg ccgggtgttt atgtttcagt agttttaaag gagtcccgta atgaaggcca
      841 gtgataaacc ccgccagctg gcggtcccct ttgcgagtac cggagataaa aaccgtatcc
      901 cggacaaggc gacacagcag accagagaga gcggtaatgc tgcgtatgat tcaggttttc
      961 ctccggtgac catgacagcg gtctcagcgg gaggtatacc gccacacggc aaggatttta
     1021 acggtctgat gtacgatatt accgcagcaa tacggttcgc ccaggctggc ggtttgtaca
     1081 cgtataatgc cggttttgcg ggggccattg gtggatatgc aaaaggagcc attctcgccg
     1141 gagtcgcaac aacagcggtc tggctgaata ccacggacga taacctgacc gatcctgaag
     1201 gctccgacag tgcgggctgg gtaaatcttc ttgaggatcc gaaaaggata ttcctgcggc
     1261 agaagaacaa tctgtcagac cttcagtata aagggacggc acgggataat cttcaggttt
     1321 acagtaaaga gcagtcagat caacgctatg ttcatcggga aggcgataaa ataaccggag
     1381 agctgaaaat ccgtggtgtt aatgcgctga ggattttcaa cgaagctttt ggcctgattt
     1441 ttcgtcgttc ggaagagtgc ctgcacctta ttcccaccag tgaaggtcag ggggaaaatg
     1501 gcgatattgg tcccctgcgc ccgttcacca ttaatttgcg gacgggtgaa atatccatgt
     1561 cgcataaagt gtctgttggc ggtggttcgc aggtcaatgg tgcgctgggt atcggcgttc
     1621 agaacgccct gggggggaat tcaattgttc ttggtgataa tgacaccgga tttaaacaga
     1681 atggagacgg tattctggat gtttatgcta atagtc
//
LOCUS       DAJTPG010000268         1707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTPG010000268 DAJTPG010000000
VERSION     DAJTPG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(110..577)
                     /locus_tag="OO230_004222"
     CDS             complement(110..577)
                     /locus_tag="OO230_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5693722.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO230_004223"
     CDS             complement(643..882)
                     /locus_tag="OO230_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5693723.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO230_004224"
     CDS             complement(1017..1622)
                     /locus_tag="OO230_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5693724.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          544 a          377 c          303 g          483 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacatt
//
LOCUS       DAJTPG010000269         1690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTPG010000269 DAJTPG010000000
VERSION     DAJTPG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            173..918
                     /gene="nfeF"
                     /locus_tag="OO230_004225"
                     /pseudo
     CDS             173..918
                     /gene="nfeF"
                     /locus_tag="OO230_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(972..1047)
                     /locus_tag="OO230_004226"
     tRNA            complement(972..1047)
                     /locus_tag="OO230_004226"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1012..1014),aa:Ile,seq:cat)
     gene            1172..1678
                     /gene="mug"
                     /locus_tag="OO230_004227"
                     /pseudo
     CDS             1172..1678
                     /gene="mug"
                     /locus_tag="OO230_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT          404 a          419 c          456 g          411 t
ORIGIN      
        1 gccggataag acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca
       61 tacttcagac atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc
      121 atttgcgaat tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa
      181 caccccccgc tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct
      241 acgcgttgag cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga
      301 cggttttaca tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc
      361 tcactttgtg ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc
      421 accgtcgcgt gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt
      481 ctttattcac gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg
      541 cgcggttcgc tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc
      601 ggaatgcccg cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa
      661 gttagtgcgc tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat
      721 ggttttaata tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag
      781 atgcaaatcc ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag
      841 aatttaagcc gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct
      901 tactggcacg caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc
      961 tttgctatat ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc
     1021 ctgctctaac cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc
     1081 aatccagcca ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata
     1141 cttttcctgg tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt
     1201 acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt
     1261 tgctcatccg gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca
     1321 gttgaagccg caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt
     1381 agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg
     1441 taagctgatt gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca
     1501 agcatatgaa cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat
     1561 tggttcgacg cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga
     1621 gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa
     1681 aacgtcaccg
//
LOCUS       DAJTPG010000270         1632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTPG010000270 DAJTPG010000000
VERSION     DAJTPG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..853
                     /gene="curA"
                     /locus_tag="OO230_004228"
     CDS             <1..853
                     /gene="curA"
                     /locus_tag="OO230_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5693725.1"
                     /translation="SYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGWQDYD
                     ISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATEPVGA
                     TVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKGIDIY
                     YENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQ
                     GFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNFGKVV
                     IRVAGDD"
     gene            1051..>1632
                     /gene="mcbR"
                     /locus_tag="OO230_004229"
     CDS             1051..>1632
                     /gene="mcbR"
                     /locus_tag="OO230_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5693726.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKA"
BASE COUNT          440 a          341 c          448 g          403 t
ORIGIN      
        1 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
       61 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      121 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      181 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      241 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      301 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      361 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      421 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      481 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      541 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      601 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      661 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
      721 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
      781 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
      841 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
      901 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
      961 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1021 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1081 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1141 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1201 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1261 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1321 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1381 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1441 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1501 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1561 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1621 gcgttaaaag ca
//
LOCUS       DAJTPG010000271         1587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTPG010000271 DAJTPG010000000
VERSION     DAJTPG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..626)
                     /locus_tag="OO230_004230"
     CDS             complement(<1..626)
                     /locus_tag="OO230_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5693727.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1312)
                     /locus_tag="OO230_004231"
     CDS             complement(740..1312)
                     /locus_tag="OO230_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5693728.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          472 a          334 c          316 g          465 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
     1321 cattgatata atatatcctt taccatgaat catcacttat attaataagt gtgtttttga
     1381 ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa
     1441 aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca agtttgttat
     1501 cagacattaa tgataattca gaatatattt atatagaaag atagatgaat ttattgatgt
     1561 cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTPG010000272         1576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTPG010000272 DAJTPG010000000
VERSION     DAJTPG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..879)
                     /gene="ltrA"
                     /locus_tag="OO230_004232"
     CDS             complement(<1..879)
                     /gene="ltrA"
                     /locus_tag="OO230_004232"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5693729.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAM"
     gene            complement(1404..1576)
                     /locus_tag="OO230_004233"
                     /pseudo
     CDS             complement(1404..1576)
                     /locus_tag="OO230_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          342 a          451 c          356 g          427 t
ORIGIN      
        1 cattgctgca tttgacgcac ccggcatgca ttttcggtac cgttcgccgt tgaaattctt
       61 cagcgtacgc actttgaagt catatccgag aaaggtgaaa ctcgttgcga cgtttctcct
      121 tttaaacgtg tcaatgtagg cgatattcgt tttccctgca ttcaggacca gcccgacttc
      181 cgagaagcgc tccgataacc tgttctttag ccttgttgca tcactcattc gtgaacaatg
      241 tactacaata tcgtcagcgt acctctcaaa cggtaccccc cgatattctc tttccatcca
      301 caaatcaaat gcatagtgaa ggaacaagtt agcaagtagc ggactaatga ccccgccctg
      361 cggagtgcct cgtgttcggg ttattaattc tccattttca caactctgca ttggcgcttc
      421 catccagcgt cgacagtaca ggatgaccca tttaggcatc ccgtggtgtt ccagagcttt
      481 gagcaccaga tcatgtctca catggtcgaa aaacgcactg atgtccactt ccagtatcca
      541 gctataacgc cagcaccgga tggcacattg tttcagagcg tcatgagctg atttgcctgg
      601 tcggtaacca tatgaatcag catggaaaat cgggtcaagc ttttcttcca tgaaaagttt
      661 tatcgctccc tgagcaattc gatcggatac tgttgggatc cccagaatgc gttccttgcc
      721 attcgacttc gggatccgtt tttccagcac tggtggcgga aaccatgttc ccgaacatag
      781 ccggttccag atcttgtata gattgccatc acgttgctgg tcgaacattt tcaaagtctg
      841 cccgtcgcat ccggggggct cccttgttgc gacgcacatc cagatatgat gcccagacca
      901 gtgcttttgg tatttcaaat gattttcgtt gcatcagtta cgtcatcccc ttttgggttg
      961 tgtcttctgc aatgaatgtg atccccatcc cttcgctcca gacccattac agatctttca
     1021 tcactactac gaatgggtcc gcccctgttt cccgatacgt gccggaaaca ggttctcacg
     1081 ttccctgaga tggtctggcc cgggatcctg ctgccttcac accggatgcc gcgcagtcag
     1141 taagcaggtg tcttctgcgc ttatcagggg acggctgtac ggccccattt tggcatcatc
     1201 taagatttcg atgcttcatc ggacagttta ctttcgttca gcttcccgaa cccacgtgac
     1261 agtagatctc tgccttttcc cgagtcgctc accacctcat tccttagaat gaagcagcac
     1321 cgggtcgttt ggtctgtgct cctgtaaacc ctgaccggag ggcccacctc tacccacctc
     1381 agagcatgga taacttatgc tgcaatttcc gaaccggcgt tacggtcatc acgaacgtag
     1441 acccacagcc gggcagtcct tgttttaccg ctgcccggct cctggaccgg aacggggata
     1501 tcatcggcat ggactttacc gggcatcagc acatactggc gcaggatgtc atacagcggt
     1561 tccagcagtt cagcaa
//
LOCUS       DAJTPG010000273         1570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTPG010000273 DAJTPG010000000
VERSION     DAJTPG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..818)
                     /gene="ipaH7.8"
                     /locus_tag="OO230_004234"
     CDS             complement(<1..818)
                     /gene="ipaH7.8"
                     /locus_tag="OO230_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5693730.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGPQIYFSMSDGQQNTLHRP"
     gene            1147..1326
                     /locus_tag="OO230_004235"
                     /pseudo
     CDS             1147..1326
                     /locus_tag="OO230_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1343..1570)
                     /locus_tag="OO230_004236"
                     /pseudo
     CDS             complement(1343..1570)
                     /locus_tag="OO230_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          473 a          254 c          349 g          494 t
ORIGIN      
        1 gggcgatgga gtgtattctg ttgtccgtca ctcatggaga agtaaatctg tgggccgtgg
       61 tagtccggcg aagaggttaa tctttgcagg gattgcagaa cgcgagtcga caatggattc
      121 cctgaaatat taatcctgta acttgaatct aattgtgaaa aactctccgg aagagtggtt
      181 atctgattaa aatgaaaata atattccttt tgtctgatat tgtttctatc agaaaattga
      241 ggaaaagtaa ccagattatt gcgctgagca cagagaattt ccagtttatc cggtaagtcg
      301 ggtaatgttg tcagcctgtt ataataaaca tgtaaaaatt tcaatttttc gggaagagcc
      361 ggtaagttat atagcctgtt attttcaaca aataatgatg ttaaatggtt tggcaaaaca
      421 ggaaggtttt ccagttgatt agaacgaaca cttaaagttg ttaatgactc aggaagttct
      481 ggtatcacag aaagcctgtt attatcagca ttaagttctt taagaaatgc aggcagcttt
      541 ggcaatgatg ttaatttatt attttctaca atcaaagcag aaatatgctt tggtaattcg
      601 ggaagagacg tcaaatttag gtctgataaa tttaatactg cttcttgatt ctgaaagcac
      661 tgggagagtc tgttaaaagc aatgcctcgc tcttccccgg gagaagagtt cttttcccat
      721 tcagtcagga ttctcagata atgttcatta ctggttgagt ttgagttaat agagggggag
      781 caagaaactg atgagtgtgt attatttaca gagaacataa tgctaggtca accttatatt
      841 tgtgagaata aatatttaaa attgtatatt tatacaaatt tcacttacca tttccgatat
      901 taagttttaa tagtttccat tttacacata acagaagtta gttgtaattt atttatggag
      961 gttttctatg gaattctttt tttattgtgg ctgcagtcaa taataacact gaattcaaca
     1021 ttccagtgag caaggaaatg taagccgagt aagtatattt gttttacatc atattttagt
     1081 caagctcatt tattcctatt atagaatgac aaattaaaat tactgtaaaa aaacgataca
     1141 catttaatac gagggaggcc ggcagaaaaa cggcttgcgg tcaggcagga gaaatgtgtg
     1201 ccgcgaatga agtccttgta tgactggata aagctccaga ggaaaacttt gtcaaaccat
     1261 gctgaaatgg taaaggcgtt caactatatc ctgaatcact ggaatgcact gaatgagttt
     1321 tgccgggtag tacttccaat cattagaata tgtgcttttc aatgaaggaa ccgatgactt
     1381 tttcgtggat ttccacggaa cgcgagaagc agattgtttt acgtgccagc cgcttgatgc
     1441 gggggcggag ggtcaggttg ttacgttcat acgttgcgtg aagattttgc cggtcaggtg
     1501 tttctcctcc ggcacttctc tggcgtagct gccccagtca tcgctggtca ccatgccgat
     1561 acagaacgga
//
LOCUS       DAJTPG010000274         1564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTPG010000274 DAJTPG010000000
VERSION     DAJTPG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..402
                     /locus_tag="OO230_004237"
     CDS             <1..402
                     /locus_tag="OO230_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5693731.1"
                     /translation="GAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATC
                     RTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKS
                     VLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            402..692
                     /locus_tag="OO230_004238"
     CDS             402..692
                     /locus_tag="OO230_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5693732.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQVAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            689..1243
                     /locus_tag="OO230_004239"
     CDS             689..1243
                     /locus_tag="OO230_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5693733.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            1384..>1564
                     /locus_tag="OO230_004240"
     CDS             1384..>1564
                     /locus_tag="OO230_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5693734.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
BASE COUNT          399 a          355 c          436 g          374 t
ORIGIN      
        1 ggggcgatca cgcctcgcga acgaaaactg gtatcaggct tcgttgatta cctgtgcgaa
       61 tcagtaggtc gggaacacac tccagccctg agtgatgccg cagagcaata ccttaataca
      121 gttgcgacat gcagaacccg ggatacggca ttgctaaagt cgtttgacgc tttccgcgag
      181 tgggtaacca ttcaggccgg attttacacc gagcatattt atcctgatgg tagtcgtggg
      241 cgcagggcaa aatctatcgc atttgcgaac atggacgaaa ccgagtttca gcaggtttat
      301 aaatctgtac tgaatgtgct gtggaactgg atcctgttcc gtaaattttc ctctccggag
      361 gaagtcgaaa atgtggccgc gcagttactg gagtttgcgt aatggtggat ttacgtaaag
      421 cggcgcgggg gcagatgtgc accgtcagaa ttcctggcta ctgcaatcac aatcccgaaa
      481 cttctgtgct ggcgcattac aggctggcgg ggacgtgcgg aacagcgaca aaaccacacg
      541 atatgcaggt agcgattgcc tgtagctcat gccacgattt aatcgacggg cgggtaaaaa
      601 ccagcgatta caccaaagaa gaattacgcc tgatgcatgc agaaggtgtt tttcgcacac
      661 aagaaatctg gagaaaggaa ggttatttat gatttaccca acaaatacag gcaaaagcgg
      721 ggaacacctt cgtctcacca cgctggaaag tgtctggatt cagggaaaac tgcgcatgtg
      781 ggggcgctgg tcgtatattg gcggcggtaa gacgggaaat atgtttaacc agttgctggc
      841 atccaaaaaa ttgacgaaaa cagccgtcaa tgaagccctg cgcaggatga aaaaagcggg
      901 aatagagaaa cctgagctgg aagcgttttt gcgagagatg atcaatggca agcaaaagac
      961 ctggctggcg cattgtactg atgcagaggc gttatgtatt gatagagtca taagtgaggt
     1021 gctggcagag catcctggat tgattagtgt cctccggcaa cggtatgaag ggcgggggat
     1081 gactaagcgt aaaatggctg aattgctgaa tgacgcacac cctgagtggt gtttccggac
     1141 gtgctgcagt cgggtagatg tatggctaaa tcttgctgaa tatatgctct atctaccgat
     1201 gcgtgatgca ttctcttccg gggatctaaa aaccgtttgt tgactcaatc tgttatccgg
     1261 ggctatattc ctcacgcgcc agcaaaatct ggcgtcggga ttggcgtccc ggatgtttac
     1321 ggagcgatat gagacgcgcc cgcgtctttt ttcatatcgt ttgcacagtc acattcgcga
     1381 tttatggcgg gctgtgtggg ggagccgaaa ggctcgccgg tttccgtacc cggttacgcc
     1441 aaccctgcac agttcgccac cagttcgatt ggcgtcgtcg gtggcgataa tttccaaatg
     1501 tacggagtta tcgttatgac cactcaaatc tctgttgaaa ctctctcccc gatcacccat
     1561 aacc
//
LOCUS       DAJTPG010000275         1506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTPG010000275 DAJTPG010000000
VERSION     DAJTPG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..226
                     /locus_tag="OO230_004241"
     CDS             <1..226
                     /locus_tag="OO230_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA esterase"
                     /protein_id="HCR5693735.1"
                     /translation="VEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(266..994)
                     /gene="tonB"
                     /locus_tag="OO230_004242"
     CDS             complement(266..994)
                     /gene="tonB"
                     /locus_tag="OO230_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5693736.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            1209..1505
                     /locus_tag="OO230_004243"
     CDS             1209..1505
                     /locus_tag="OO230_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5693737.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          353 a          359 c          394 g          400 t
ORIGIN      
        1 ggttgaagga atgactttct tacggccggt tgcggtcggc gatgtggtgt gctgctatgc
       61 acgctgtgtc cagaaaggga cgacatcggt cagcattaat attgaagtgt gggtgaaaaa
      121 agtagcgtct gaaccaatcg ggcaacgcta taaagcgaca gaagcattat ttaagtatgt
      181 agcggttgat cctgaaggaa aacctcgcgc cttacctgtt gagtaatagt caaaagcctc
      241 cggtcggagg cttttgactt tctgcttact gaatttcggt ggtgccgtta attttaaaca
      301 ggatattcac cacaatccca ctgcctggct tacccggctc ataacgccat ctgcgcatcg
      361 catttttcac ctcacgctca aacatgttcg caggcttggc tgagaggatt tgtacgttat
      421 ccacgcgacc atccggcgtg acgtcaaatt taactttaac ctgcccttca atgcgcaatg
      481 cctgtgctcg tgccggatac tgcggctgat tacggcttaa tgcgcgtggt cctgaagcca
      541 cactggtaac cggcttgctg gttgcagccg ttgccgtact tgatgtgggg cgcgcgggtg
      601 ccgtattttc aaacggtgat gccggacgcg actctacggg tttgacatcg cgttttggct
      661 gctcctgtac ctttttcacc ggcttcggtt ttggcttagg tttcggcttc ggcttttcaa
      721 tgaccaccgg tgcttctttt ggcggttcgg ggatcggctc aggttccggt tctggctcta
      781 ccaccggctc cggtggcggc tgaacggctt gtggcggttc gagatcagca ggcgcaacca
      841 tcgtgacaga aattggttgc gcaggcgcag gtagttcaat aacctgatgt accgaggtat
      901 agagcagccc cgccacaaca gcaccatgaa tgcagaccga aagtaacgtc ggccagggga
      961 agcggcgagg taaatcaagg gttattgaag tcataatcat ttcagtagaa aaactaggcc
     1021 tcgattttaa atgcaaatag caatcatatt caataaggca agacgatcag gatcagtgaa
     1081 aatgtcattt ttggcgtaat tacccttact ttatgcatga ccttaacaaa gacatcatct
     1141 ttccgttgca gatcttcgcc ctttcaaata acgtacttta caactttccc gaacaaggag
     1201 ttgtgcccgt gttgtatgtt atttacgctc aagacaaagc tgatagcctc gaaaaacgcc
     1261 tttccgttcg tccggcacat ttagcacgtt tacagttact gcatgatgaa ggtcgtttgt
     1321 tgacagctgg tccaatgcca gcggtagaca gtaacgatcc aggggctgcg ggatttactg
     1381 gctcaaccgt tattgctgaa tttgaatcgc tggaagcagc tcaggcctgg gccgatgctg
     1441 acccgtacgt agcggcagga gtctatgaac acgtttcagt gaaaccattt aagaaagtgt
     1501 tctgag
//
LOCUS       DAJTPG010000276         1497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTPG010000276 DAJTPG010000000
VERSION     DAJTPG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..586
                     /locus_tag="OO230_004244"
     CDS             <1..586
                     /locus_tag="OO230_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5693738.1"
                     /translation="TNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYRKNTLS
                     YRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHRSSYLV
                     SRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYLAKREQ
                     KECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            959..1342
                     /locus_tag="OO230_004245"
     CDS             959..1342
                     /locus_tag="OO230_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693739.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          369 a          397 c          386 g          345 t
ORIGIN      
        1 caccaatccc gcttacgaaa tggctcccca gctcctggag cactttaacc agcatctgga
       61 ttcattattc ggggtctatt cgaaactgtt accgttcaga atggattttg cttaccgaaa
      121 aaacaccctc agctacagat gtgcctgcag gtacgccatg tgcgcggaga tgctgcaact
      181 gattaacgag gtgggagaga aactggtcgg gtatgcctgg gtgatggaat atacagaacg
      241 taaagggctt cacattcatt ttgtgggcta tctgaacggc cagagccaca ggagttcgta
      301 tctggtatcc agattgatgg gagatatctg gaggcgggtt accgagggta atggttacta
      361 ccactggtgc cggtttaata aaaattatcc ggtaaacatt aaccacgtca ttcactattc
      421 agatcacaaa gcggtgaatg atttacgcta tgccatcagc tacctggcca aacgggaaca
      481 gaaagaatgt ggcatcatcc tgaaatgcag cgggcttcct gaaaaaagta accggggtcg
      541 accacgactg gacagccccc tgccggggat atgtgctctg gtgtaaaccc ctgaacgaca
      601 agtacgggct ggcgctaatc tttgctgtcg gttccggcgg ccacttcagc ggcactgcgc
      661 attttttacg gcgtggtcat tccgtatgaa ccatacggag atttaaccat gacacattct
      721 ttaccattcc ttccggcaac cgatacagca catcccccaa tctccatctc tgcttactga
      781 ctgtttttta cgtcagtggg ccattgtgtg acaaccacag tgctgtaaca gcaaccagcc
      841 tgctttaccc aaactccagt ccagatttta actgaaccgc cattcacacc ctgaccacgc
      901 tgcccgtacc gggacggctg ttgcctgcgt gtcgtctggc ggctgaggaa catatactat
      961 gtacgcaaaa tcttttctcg cacttgatgg caacggacgt ctgaccggtg cccgtaccgc
     1021 acagactgca ccttacgata gctacacctg ccacctttgc ggcagtgcac tcagatacca
     1081 cccgcaatac gacactgaac ggccctggtt tgaacacgct gacgaagggc tgacggagca
     1141 cggtcatgag tgcccttatg tcaggccgga acgcagagaa atacggttga ttaaacgtct
     1201 gcagcaattc gtcccggata ccttacccgt agtacgtaaa gccagctggc actgcaggca
     1261 atgtcaccac gattattatg gggagcagta ctgcacacac tgccagaccg ggcgcttcag
     1321 tgaggagggg ggagcagaat gaacggtata aaaacggggc tggggatcac gccgggtgag
     1381 cacattatca gtgccgactc tgcgctcagc agaaatatcc ggcagtgttt ctgcctttcc
     1441 tgccatggcc ggttgattct gcagacagat gctcaggggg cgtggtttga gcatgat
//
LOCUS       DAJTPG010000277         1474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTPG010000277 DAJTPG010000000
VERSION     DAJTPG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(5..184)
                     /locus_tag="OO230_004246"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="OO230_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="OO230_004247"
     CDS             708..992
                     /locus_tag="OO230_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5693740.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="OO230_004248"
     CDS             992..1267
                     /locus_tag="OO230_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5693741.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          402 a          369 c          361 g          342 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga catt
//
LOCUS       DAJTPG010000278         1442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTPG010000278 DAJTPG010000000
VERSION     DAJTPG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..169
                     /locus_tag="OO230_004249"
     CDS             <1..169
                     /locus_tag="OO230_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5693742.1"
                     /translation="TRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMND
                     EQIAMLNARLS"
     gene            601..>1442
                     /gene="sitA"
                     /locus_tag="OO230_004250"
     CDS             601..>1442
                     /gene="sitA"
                     /locus_tag="OO230_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5693743.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPT"
BASE COUNT          429 a          333 c          301 g          379 t
ORIGIN      
        1 tacgcgacat acttttgcct gctggctgtt gacggcagga gcgaacccgg catttatagc
       61 cagccagatg gggcatgaaa ctgcgcaaat ggtgtatgaa atttacggta tgtggattga
      121 tgacatgaac gacgaacaga tagccatgtt gaatgcgcgg ttatcgtagt tgcaaagttt
      181 gcccccaatt tgccccattt agtaccagag aactgaaata atgcaagaaa atcaacaaat
      241 tacaaagaaa gaacaataca acctgaacaa gtaagggcaa aaatcacaac tatctgactc
      301 ataagtattt tatttatttt tcaatatgtt aaacattatc agcgacaaca ataagctacg
      361 ataacccact cttttttgcc ccatttttgc catcaacaat tttcctcata aagtcatttc
      421 aaagcctaat agctgctcta cttttttcct caatatcatg tcgccagcag gaaacaacct
      481 aatccctgtt ttaggcctca aagctgttaa tgataattat tttcattttg tataggttat
      541 gctataaaac atagccgttg ctatgtttgt tctaattttg ctcactatag gtactaaatt
      601 atgcactcaa taaaaaaagt aaccatgctc ttggggtgcc tcgccctcac ctgctcgatc
      661 gcatttcagg caagtgcaac tgaaaaattc aaggtcatta caacatttac catcatcgcg
      721 gatatggcca aaaacgtggc tggagatgct gcagaagtct catccataac caagcctggt
      781 gcagaaattc atgagtatca gcctacccct ggcgatatta aacgtgcgca gggggcacaa
      841 ctgattctcg ccaatggtat gaatctggaa ttgtggttcc aacgctttta ccagcatctc
      901 aatggggttc cagaagtaat tgtctcttcg ggtgtgacgc cagtagggat caccgaagga
      961 ccctatgagg gcaaacctaa tccccatgca tggatgtcgc cagataatgc tctgatttac
     1021 gtcgataata ttcgtgatgc gttgataaaa tacgatccgg caaatgcaca aacctaccaa
     1081 cgtaatgccg atacttataa agccaagatt acccaaaccc ttgcccccct gcgtaagcag
     1141 attacggaac tccctgagaa tcagcgatgg atggtcacca gtgaaggggc tttttcttat
     1201 ctcgcacgcg atttggggct aaaagagctt tatctgtggc cgattaatgc cgatcaacaa
     1261 ggaacaccgc agcaggtacg taaggttgtt gatatagtta agaaaaatca tatcccggca
     1321 gtctttagcg agagtacgat ttccgataaa ccagcgcgtc aggttgcgcg tgaaaccggc
     1381 gcacactacg gcggtgtgct ctatgtcgat tccctgagca cagaaaacgg cccggtacct
     1441 ac
//
LOCUS       DAJTPG010000279         1429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTPG010000279 DAJTPG010000000
VERSION     DAJTPG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..378
                     /locus_tag="OO230_004251"
     CDS             <1..378
                     /locus_tag="OO230_004251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693744.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO230_004252"
     CDS             complement(454..918)
                     /locus_tag="OO230_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5693745.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..>1429)
                     /gene="nanK"
                     /locus_tag="OO230_004253"
     CDS             complement(915..>1429)
                     /gene="nanK"
                     /locus_tag="OO230_004253"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5693746.1"
                     /translation="TDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLADPHGPV
                     CGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSARTLAR
                     LIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHDAGLLG
                     AALLAQGEKL"
BASE COUNT          377 a          396 c          321 g          335 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggtta
//
LOCUS       DAJTPG010000280         1406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTPG010000280 DAJTPG010000000
VERSION     DAJTPG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..169
                     /locus_tag="OO230_004254"
     CDS             <1..169
                     /locus_tag="OO230_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5693747.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            154..792
                     /locus_tag="OO230_004255"
     CDS             154..792
                     /locus_tag="OO230_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5693748.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..>1406
                     /locus_tag="OO230_004256"
     CDS             1020..>1406
                     /locus_tag="OO230_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693749.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          348 g          394 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttc tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg actctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tgcaggaaaa cgcctgaggg gctggttgcc tgtagcctgc
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata agatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagt
//
LOCUS       DAJTPG010000281         1385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTPG010000281 DAJTPG010000000
VERSION     DAJTPG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            400..1149
                     /locus_tag="OO230_004257"
     CDS             400..1149
                     /locus_tag="OO230_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5693750.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          392 a          283 c          277 g          433 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagaccta
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctc
//
LOCUS       DAJTPG010000282         1291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTPG010000282 DAJTPG010000000
VERSION     DAJTPG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(1..264)
                     /locus_tag="OO230_004258"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="OO230_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO230_004259"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO230_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5693751.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1170..1291)
                     /locus_tag="OO230_004260"
                     /pseudo
     CDS             complement(1170..1291)
                     /locus_tag="OO230_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          379 a          318 c          205 g          389 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt t
//
LOCUS       DAJTPG010000283         1282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTPG010000283 DAJTPG010000000
VERSION     DAJTPG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..398
                     /locus_tag="OO230_004261"
     CDS             <1..398
                     /locus_tag="OO230_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5693752.1"
                     /translation="IAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYW
                     FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAK
                     SLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            422..>1282
                     /locus_tag="OO230_004262"
     CDS             422..>1282
                     /locus_tag="OO230_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5693753.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSETAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT          329 a          311 c          371 g          271 t
ORIGIN      
        1 aaatagctta caccggagat ggtcgcgtgg tgtgggtcgg taaggatgtg acaggcatcg
       61 agccacgcaa tgccagcgtt atcgaagttc cggatattac cgctaaccga cggattaccg
      121 cgccgggtta ctggttttac cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg
      181 aagatgagcg tgatgccctt ctggctcagg tcagtgcccg gacaggggaa tgggaagaag
      241 acctgctgct ggggttaatc agcgacgaag ataaagaaaa gctgaaagcc tgtcgtattt
      301 acgcgaaatc gctgcaggcg atggatttca gcaccatcac tgataaatcc tcatacaacg
      361 ccattgaatg gcccgcctct ccggaaggtt cttcctgatt taatttatcg cgagaaaaac
      421 aatgtctgta gtgatatcag gtgcgctgac tgatggcgca ggtatcccca tgtccggata
      481 ccatattatt ctgaaatccc gggtaaacac cccggaagtg gtgatgaaca ctgttgctga
      541 tgtgatggca ggaaacgatg gtgaatactg tttccatgcg cggactggaa aatatggtgt
      601 gtatctgaaa caggactggc gcaacgagta caacgttggc gacattgctg tatatgagga
      661 ctcaaagccc ggcacgctga atgactttct gattgctcct gatgagggcg acctgaaacc
      721 ggatgtcgtc aaacgctttg aggaaatggt ggcgcaggcg cagcagagcg ccggggccgc
      781 agccggaaac gcacagcaga cggcgcagga tgtggcggca gccgcaggtt atgcccgcgc
      841 agcagagcag gccaaaaatg acattgatgc tgcgctgacc ggcactctga aaacggctaa
      901 ccatctgtct gaaaccgcag cagcaggcga aaaggcacaa cagaagtccc gggataatct
      961 ggggctgaaa agtgcggcca cgatggaagc acagagcgac atttacgacc ggacaaaagg
     1021 ccgtctggcg atacccggcg cattcggctt tgggtgtgct tttctgcctg aagatgttat
     1081 ccgttttgac actaagagtg atttcctggc ctgggtaagg aatgcgctgc caggtgaata
     1141 ttccgttgct ggcccctacg gcatcatcat acccgacaca cggtttgaag gggtgctcag
     1201 catccggtgg actgatgcac gccctgagac aacagaaccg cggtacagag ccaaatccct
     1261 tactttttac ggcattaacg gc
//
LOCUS       DAJTPG010000284         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTPG010000284 DAJTPG010000000
VERSION     DAJTPG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO230_004263"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO230_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5693754.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAJTPG010000285         1218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTPG010000285 DAJTPG010000000
VERSION     DAJTPG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>1218
                     /locus_tag="OO230_004264"
     CDS             <1..>1218
                     /locus_tag="OO230_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5693755.1"
                     /translation="IGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALAL
                     IKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQS
                     FVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNM
                     QDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSS
                     LNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALS
                     PAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGAK
                     VILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWS
                     AENSVTMSSKVSGM"
BASE COUNT          366 a          279 c          297 g          276 t
ORIGIN      
        1 atcggcctga tggatactgt cgccacggtc acgccggcac aattgcgcca attttatcaa
       61 cgctggtatc aaccaaataa tatgaccttt atcgtggtcg gcgatatcga cagtaaagaa
      121 gcgctggcgc tgataaagga taatttaagt aagcttccgg ctaacaaagc agctgaaaat
      181 cgcgtctggc cgacaaaagc cgaaaaccac ctgcgcttta atatcattaa tgataaagaa
      241 aaccgggtga acggcatcgc actctattat cgcctgccaa tggtacaagt gaacgatgag
      301 caaagctttg tcgaacaagc tgaatggagc atgttagttc agctgttcaa tcaacgtctg
      361 caggaacgca tacagtcggg cgagttgaag actatttctg gcggcactgc gcgcagcgtt
      421 aaaattgcac ccgattatca gtcgctgttt ttccgtgtaa atgcacgaga cgataatatg
      481 caggatgctg cgaatgcatt aatggcagag ttggcaacca ttgatcagca tggtttttct
      541 gctgaagaac tcgatgatgt caaatctacc cgcctcacct ggctgaaaaa tgcggttgat
      601 cagcaagctg aacgtgattt acgtatgctg accagtcgcc tggcatccag ctcattaaat
      661 aatacgccgt tcttgtcgcc ggaagagaca tatcaacttt cgaaacgtct gtggcagcaa
      721 attaccgtgc aaagtctggc ggaaaaatgg cagcagttaa gaaagaacca ggacgcattt
      781 tgggagcaaa tggtaaacaa tgagcttgcc gccaaaaaag cattgtctcc tgcggctatc
      841 ctggcgctgg aaaaagagta cgccaacaaa aagctggcgg cttacatctt cccaggcaga
      901 aatttatcgt taacagtaga cgctgaccca caggcggaaa ttagcagcaa agaaacgctg
      961 gctgagaatc tgacatcatt aacactttcc aatggtgcca aggttattct ggcaaaatcc
     1021 gcgggtgaag agcaaaagct acaaattatt gccgtatcga ataaaggcga tttaagtttc
     1081 cctgcgcagc aaaaatcact tatcgcgctg gcaaataaag cagttagcgg aagcggcgtt
     1141 ggcgaactct cctcttccag cctgaaacgc tggagtgcgg aaaactcggt aaccatgagc
     1201 agtaaagtca gtggcatg
//
LOCUS       DAJTPG010000286         1154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTPG010000286 DAJTPG010000000
VERSION     DAJTPG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..648)
                     /gene="yidZ"
                     /locus_tag="OO230_004265"
     CDS             complement(<1..648)
                     /gene="yidZ"
                     /locus_tag="OO230_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5693756.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTW"
     gene            complement(623..>1154)
                     /gene="mdtL"
                     /locus_tag="OO230_004266"
     CDS             complement(623..>1154)
                     /gene="mdtL"
                     /locus_tag="OO230_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5693757.1"
                     /translation="SVILTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTP
                     FALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQAL
                     GPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFV
                     APGRPVAAHEEIHHHA"
BASE COUNT          305 a          323 c          292 g          234 t
ORIGIN      
        1 ccaggtgtcg ctctgttccc agcaaatgct gatatgcgga taacgtaaaa aggtgtccag
       61 attccacgtt tcatgcagtg ccggatgatc tttgcgtaac cagacgcagg gtacatcact
      121 aaacagcact tcataatcaa tggctaacgg tagcgagctt aacagctccc gcgagcgagg
      181 atggctttcg cgaccggaaa aaccaatatc cacttcacca cgagtaatgg catctaagga
      241 atcgtaatcc cagttacgta gtttgatggt cgcctgcggg taacgttggt agatccgttt
      301 cgacagcgca ttaagcatga tcatcatcag cggcgattcc gccgccaact caaacttcag
      361 gccgcgcggt gtctggtggt gcggtttatc cagcagcaga ttgctcattt gcatccattc
      421 cgccagattt tgctccatgc tgaccattag ctgtgtgggc gacagcccca gcggtgagtt
      481 cacaaagagc gggtcgtcaa accacgctct taactttgcc agcgacttac tcaccgctga
      541 aggggtcacg ttcatccgct tcgccgcttt ggtcacgctg cgctcctgca tcagcagttg
      601 cagacagagc agcagattga gatcaagcgt ggtgatggat ttcttcatga gcggcaacgg
      661 ggcgtccagg cgcgacgaac ataatcagca acaggctcac tatgctacag gcaatcagaa
      721 tcccgatcag catattccat gcgccgatac caaccaccgc ggccagccaa atccacagtg
      781 acgaaccgca aacctgcgca atacctaagg tcgagctggc tacgcccgcg cgtaatgaaa
      841 atggtcctaa cgcctgactc atcgctacgc caaaacctac cgagaaaccg gcgcaaatca
      901 gcgtgatacc aaacagagaa accgcatggg aaggtgaaac ggcaagggtg atccccgccg
      961 caaggaataa cacctgcgag gtgatcatca acgtacgtgg cttaaaaatt cccagcgcaa
     1021 atggcgtcga gaatgaaaag gtcatgctga cgccagcggt cagcgccata atggtggcgt
     1081 attctccgcg ctcaaacccc atgatttcca tcagcaatac cggcgatgta ttgacgaaag
     1141 tgaggatcac cgaa
//
LOCUS       DAJTPG010000287         1151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTPG010000287 DAJTPG010000000
VERSION     DAJTPG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            353..>1151
                     /gene="ipaH1.4"
                     /locus_tag="OO230_004267"
     CDS             353..>1151
                     /gene="ipaH1.4"
                     /locus_tag="OO230_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HCR5693758.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          398 a          233 c          194 g          326 t
ORIGIN      
        1 aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaaata gagcagtaat
       61 tctatttcag agaggagtaa ctccatctat cctcgatacg tactgaacgt tatcgtgata
      121 tattcatcat gctttcttca ccattattcg agtataggga gagacgcaat gaccttaaat
      181 acctttttat ggctgaatta ccattctcag gaataacagc agactccttc ccatagacgg
      241 cttaggggga aagcaatacc cggagaaagg gtacaaagca aactgtttta aaattaataa
      301 gccatattct atttaactag aattcttatc gttgataaga attctagtta taatgattaa
      361 atcaaccaat atacaggcaa tcggttctgg cattatgcat caaataaaca atgtatactc
      421 gttaactcca ttatctttac ctatggaact gactccatct tgtaatgaat tttatttaaa
      481 aacctggagc gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa
      541 taggctgaaa atatgtttac aaaatcaaga ggcagaatta aatttatctg agttagattt
      601 aaaaacatta ccagatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt
      661 aacacatctc cctgatttac caccaatgct taaggtaata catgctcaat ttaatcaact
      721 ggaaagctta cctgccttac ccgagacgtt agaagagctt aatgcgggtg ataacaagat
      781 aaaagaatta ccatttcttc ctgaaaatct aactcattta cgggttcata ataaccgatt
      841 gcatattctg ccactattgc caccggaact aaaattactg gtagtttctg gaaacagatt
      901 agacagcatt cccccctttc cagataagct tgaagggctg gctctggcta ataattttat
      961 agaacaacta ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct
     1021 gacaacactt ccggaaagtg tcctgagatt agctcagaat gccttcgtaa atgttgcagg
     1081 taatccattg tctggccata ccatgcgtac actacaacaa ataaccaccg gaccagatta
     1141 ttctggtcct c
//
LOCUS       DAJTPG010000288         1122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTPG010000288 DAJTPG010000000
VERSION     DAJTPG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            127..>1122
                     /locus_tag="OO230_004268"
     CDS             127..>1122
                     /locus_tag="OO230_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5693759.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRRE"
BASE COUNT          358 a          260 c          177 g          327 t
ORIGIN      
        1 atatacttac cagggtagaa agcatgatat aacactcatt cccttatggc aaatattctg
       61 aaacaccctc ctcattagga gagatacatt aaatacaatt tattcacaat ctaattccat
      121 atccatatgc gagaaattaa tatgctcaga aacatttcat cctgtttatt tccacatatc
      181 agcacaatta catcccccaa ccattatttg tccgaatggg atgattggga gaaacagggg
      241 ttaccggaag aacagcgtac tgaggcggta agaagacttc gtgcatgtct tacctctaag
      301 gggcataaac tggacctgcg agccttggcg ctttcctcgc tacctgtact ccctgcttgc
      361 attaaaaagc ttgatgtgag ctgtaataaa ttaaccatcc ttactgattt acctgaaaat
      421 attaaagaac ttattgcaag agataatttc ttaacacata tatctgcatt accacattat
      481 ctaataactt tggatgtgtc cgaaaatcaa ttagagaatc tgccgttatt accagacacc
      541 atcaaatcac taagcgcaga gtataatagg ttatccacac tgccttcatt acccttgaat
      601 ttaaaaaaac ttgaggttag gaacaacgaa ctgcaaactc ttccatctct gccttctaat
      661 cttaagatac ttaaggttgc gcacaaccat cttactgaac tgcccccttt acctaggaga
      721 ctgcaacttc tttttgcata tagcaataga ttaagcaact taccaaacat ccaagaaaat
      781 attatcatga gaagattttt ttattttgaa aacaaccaaa taactacaat cccgacaaat
      841 ctttttcgtt tagatcctca tataactatt gagattgcaa ataacccctt atcagatcaa
      901 actctgctat tcttaataca gcaaacttcg gttccaaatt ttaacgggcc tcagtttcgt
      961 atttccctgt cagaccaaaa cagactgttt ttacgccaga tgttgccgca aaatttacat
     1021 tcgcgccata tcagagtcat cactgaaggg gggcagaact ttcagatccc ccctcttccc
     1081 gaaactgtgg cagcctggtt tcctgaagca gatcgtcggg ag
//
LOCUS       DAJTPG010000289         1112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTPG010000289 DAJTPG010000000
VERSION     DAJTPG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            57..554
                     /locus_tag="OO230_004269"
                     /pseudo
     CDS             57..554
                     /locus_tag="OO230_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            595..786
                     /locus_tag="OO230_004270"
                     /pseudo
     CDS             595..786
                     /locus_tag="OO230_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(880..>1112)
                     /locus_tag="OO230_004271"
     CDS             complement(880..>1112)
                     /locus_tag="OO230_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR5693760.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT          296 a          279 c          297 g          240 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttggag catcatgctt
      601 catgaagaaa aactggcacg tcatcaacgt aaacaggcga tgtatacccg aatggcagcc
      661 ttcccggcgg tgaaaatgtt cgaagagtat gacttcacgt tcgccaccgg agcaccgcag
      721 aagcagctcc agtcgttacg ctcactcagc ttcatagagc gcaatgaaaa catagtctta
      781 ctggggacat ctgacatcac aaatccccgg gtggggattt gtgtataaga gacagcccat
      841 ggaggaagga gatatatcgc agctctgacg actcactcct tatttacccg ggaaatactt
      901 ctccagcaat gcatacagga tggcagcggt ttcagcatcc attatgccgt cataattttt
      961 ctgccggaaa tgaagctgaa aagcgcggac aagcgcctta tatccgtcat ccgtctccac
     1021 accagaaata ctgtatccat aggtcttcag ccgtgacacg atatcaacct tatccggaat
     1081 gccctgcgat tcaaaaaata aatatttagt ca
//
LOCUS       DAJTPG010000290         1099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTPG010000290 DAJTPG010000000
VERSION     DAJTPG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..1009
                     /locus_tag="OO230_004272"
                     /pseudo
     CDS             1..1009
                     /locus_tag="OO230_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          310 a          269 c          257 g          263 t
ORIGIN      
        1 caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg aagagtttta
       61 ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac ccgaatggca
      121 gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga taaccgctcc
      181 tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac gtgagtttgc
      241 cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg cggatgagtc
      301 ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg acgataaacc
      361 atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt acgactggaa
      421 agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc cactgtataa
      481 cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac cttttggccc
      541 ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct gggctgctat
      601 gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg acaatcattt
      661 ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat atgcattatt
      721 gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa tcgccattta
      781 tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag gggacattgg
      841 cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata acgattactg
      901 gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt ataacgaacg
      961 gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac gcaggattta
     1021 acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa tgaaatccgc
     1081 atggcgatcc atcaggtta
//
LOCUS       DAJTPG010000291         1088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTPG010000291 DAJTPG010000000
VERSION     DAJTPG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO230_004273"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO230_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5693761.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          347 a          158 c          194 g          389 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gggtaatgct gccaacttac tgatttagtg tatgatggtg attttaaggt
     1081 gcttgcgt
//
LOCUS       DAJTPG010000292         1082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTPG010000292 DAJTPG010000000
VERSION     DAJTPG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(21..824)
                     /gene="dkgB"
                     /locus_tag="OO230_004274"
     CDS             complement(21..824)
                     /gene="dkgB"
                     /locus_tag="OO230_004274"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5693762.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(987..1063)
                     /locus_tag="OO230_004275"
     tRNA            complement(987..1063)
                     /locus_tag="OO230_004275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Asp,seq:gtc)
BASE COUNT          283 a          253 c          247 g          299 t
ORIGIN      
        1 ggaggagctg tcagagaggc ttaatcccat tcaggagcca gaccttccgg gctaaccagg
       61 cggtcgttgc aatccagtgc ggcgatcgct tttttatctt cggcatcaag ctgtaaattt
      121 tgtgccttaa gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa
      181 ccttccccca tagcccacgc cagaatcact tgtgccggaa tcgcattgtg tttagctgca
      241 atacgagcaa taacctcatc tttcagtgcc ttaccataag ccagcgtcat ataggaagtg
      301 atatgaatac cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag
      361 agttcaatct ggttggtagc gatgttttca gcgccaacag cagcaatcac tttttccatc
      421 aatgggatcg tgaagttgga aataccgatc tcacgcgtca gcccttgttt tttggcttcc
      481 agcagcgcct gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt
      541 agcgtcagat caacataatc ggtacgcaat ttttgcaggc tctctttcag actcgggatc
      601 aatttgtctt tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt
      661 ggcacgccac tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgcg
      721 gtatcaattg cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg
      781 tctttcagac ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa
      841 tgctattttc gttaactgag gtgattatgg gcttcagact gttgcgggaa aagagcgtaa
      901 aacgcagagg atttttgcga ttctagcaat aatagatatc cagacgcaaa aaaacatcga
      961 gcgttaactc gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc
     1021 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa
     1081 cc
//
LOCUS       DAJTPG010000293         1081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTPG010000293 DAJTPG010000000
VERSION     DAJTPG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..185)
                     /locus_tag="OO230_004276"
     CDS             complement(<1..185)
                     /locus_tag="OO230_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5693763.1"
                     /translation="MLTSEQHRCHNEKRNFSAEFKRESAQLVVDQKYTVADAASAMDV
                     GLSTMTRWVKQLRDERQG"
     gene            371..>1081
                     /locus_tag="OO230_004277"
     CDS             371..>1081
                     /locus_tag="OO230_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5693764.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTT"
BASE COUNT          314 a          200 c          225 g          342 t
ORIGIN      
        1 ccctgccgct catcacgtaa ttgtttcacc catcgcgtca ttgtggaaag gccgacatcc
       61 atagcgctgg ctgcatctgc cacggtgtat ttctggtcaa cgaccagttg agcggattcg
      121 cgtttaaact ctgcgctgaa atttcttttt tcattatggc acctgtgttg ttctgaggtg
      181 agcatatcac ctctgttcag gtagctaaat ttagtgtgcc acctcaaggt tcttagtgag
      241 ccgtttttaa aagatatgat aatatattat tgtttataaa ttgttgatgt ttgatttagt
      301 agtatataaa gttcaccacc tttattaggc tttcctttta tctctactta ctgggtgcca
      361 gaaactttaa attttaaacg gagggggcat tttgtttaaa gcaaatcatg ttccggtatt
      421 aatgtatcat catgtcagcc attgcccagg actggttaca ctttctcccg agacattccg
      481 gaaacaaatg aaatggttgg ctgaaaacaa ctggaaaaca ctgtcatcag atgaactgga
      541 gtttttttat cggggcggaa aacttccccg taaaagcgtt atgttgacat ttgatgatgg
      601 ataccttgac aactggtttc aggtatatcc tttattaaac gagtttaatc tcaaggcaca
      661 tattttttta attaccagtt ttattggaaa tggtccggtt cgtcactctc ctggaaagga
      721 atattctcat cgcgattgcg aacatcaaat agcaaccggg aatgctgata atgttatgct
      781 tcgctggtct gaagtgaacg aaatgttgca atctggtctg gtcgaatttc atgttcatac
      841 acatactcat acacgttggg ataaaaagtt cacttcacga gaagaacagt gtaaacatct
      901 tcggcaggac ttattgagcg ggagagaata tttaaaaaaa atgacaggaa aatgcagtaa
      961 acatttgtgt tggccggaag gttattataa caaggattat attcagatcg ctgaagaact
     1021 tggattccat tatttatata caacagaaag aagaatgaat gctcctgcta aaggaactac
     1081 a
//
LOCUS       DAJTPG010000294         1064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTPG010000294 DAJTPG010000000
VERSION     DAJTPG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..534
                     /locus_tag="OO230_004278"
                     /pseudo
     CDS             1..534
                     /locus_tag="OO230_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          331 a          232 c          218 g          283 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatacaaa aacaaataat
      721 accgttgcaa taattccttt tagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca aggaaatatt
      841 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      901 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
      961 ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaatatatta cgcgtaacaa
     1021 actcatcttc ttctggcata agtgaaaaac atttagatca ctgt
//
LOCUS       DAJTPG010000295         1050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTPG010000295 DAJTPG010000000
VERSION     DAJTPG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..76)
                     /gene="hokD"
                     /locus_tag="OO230_004279"
     CDS             complement(<1..76)
                     /gene="hokD"
                     /locus_tag="OO230_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5693765.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVT"
     gene            complement(304..969)
                     /locus_tag="OO230_004280"
     CDS             complement(304..969)
                     /locus_tag="OO230_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5693766.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          265 a          261 c          237 g          287 t
ORIGIN      
        1 tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg attaacatcg
       61 ccttttgctg cttcatagcc tgcttctcct tgcctttcgg catgtaagag gctaacctac
      121 atgtgcaaag catgaaattg gcctcagatt aatgttaagc gtcttgccgg acgcgtaatg
      181 ttaactgggg cttttctcta tctgcctttt ggtgttcatg cctgaagcag atagcctcaa
      241 gcacccgcag tcattctact tacctcgcca atatgaaatc aatcagaaag gtgccccata
      301 aaatcactcc ttctcttctt taccgtagtg gagttgacca attttgataa gagggcgtcc
      361 ctgagatttg cggtgtagat tggtatcgcg cagagaatac acacagccac aatattcctg
      421 ctgatagaat ttttcgcgct tgctgatttc aatcatacgg gacgagccgc cctgcttgcg
      481 ccagttataa tcccagtaca ccatgcccgg ataatgcgcg acggctcgcc gcccacagtc
      541 gttaacctgc tgcatatttt tccagcgtga aatgcccagt gaactgctga tcacactgaa
      601 accattttca gcggcgtaca gcgctgtccg ctcaaaacgc atgtcaaaac acatggtgca
      661 acggatccct cgttcgggct cccattccat tcctttggct cgttcaaacc agttgtctgt
      721 gtcgtaatca gcatcaataa acggcacgcc gtgttgttca gcaaagcgaa tattctcatc
      781 cttacgaatt aaatactctt tctgaggatg aatgttcggg ttgtagaaaa agatggtgta
      841 gtcgatttcc gaggcctgaa gcgcctccat cacctcaccg gaacatggag cacagcaaga
      901 gtgcagtagt agtttgtttg ccccgtttgg gagctccaat ttgggccgtt tgaaatcagc
      961 aactgtcata aatatgttta ttgaggtcat aaaaatatca aagagtgtag cattagaacg
     1021 gggctatcgg aaacagatgt gtgactgctc
//
LOCUS       DAJTPG010000296         1008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTPG010000296 DAJTPG010000000
VERSION     DAJTPG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..168
                     /locus_tag="OO230_004281"
     CDS             <1..168
                     /locus_tag="OO230_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5693767.1"
                     /translation="NMDDLVTVVALVTLHTDALHATRDEPLAFVPPGMAFRVFAGVAA
                     GMTERGLARMQ"
     gene            180..551
                     /locus_tag="OO230_004282"
     CDS             180..551
                     /locus_tag="OO230_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5693768.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            538..>1008
                     /locus_tag="OO230_004283"
     CDS             538..>1008
                     /locus_tag="OO230_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5693769.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          228 a          226 c          312 g          242 t
ORIGIN      
        1 aatatggatg atctggttac agtcgtggca ctggtgacgc tgcataccga tgcacttcat
       61 gccacgcggg atgaacctct ggcatttgtg ccgccgggaa tggcgttccg tgtctttgcc
      121 ggtgtggcag ccggaatgac agaacgtggc ctggccagaa tgcaataacg ggaggcagcg
      181 tggctgattt cgataatctg tttgatgttg cacttgatct cgcagacaag gccattattc
      241 gcaatatggg gattagagcg gtcattacgt caggccggct aaaagggatc atgatttccg
      301 gggtttttga tgatcctgaa aatatttctc tggtggccgg cggtgtgcgt attgaagact
      361 ctttaccatc cctgtttgtg aaaacagcag atattttacg gctgtgtcgc aatgattcgc
      421 tgatgattgg tcgtgagtct ttctgtgtgg atcgtatcac ccctgatgat ggcggatgta
      481 gttatatccg gttgcggcgt gaggggctgc cgggaaacgt aagggcagga cgatattatg
      541 aaggggcttg agaatgccat ccgcaatctg aacagccttg atacccgtat ggtgccacag
      601 gccagcgcat gggcgataaa ccgtgtggca cagaaagcgg tctcggttgc cacccggcag
      661 gttgccggga ataccgttgc gggcgataac caggtgaaag ggatccccct gaaactggta
      721 cgtcagcgtg tccgggtgtt taaagccagt ccgtcaggaa aaatgacggc caggatccgc
      781 gttaaccggg gcaatctgcc cgccatcaaa ctgaacacaa cacggcggcg tgctggtgaa
      841 ggactgagag tgggaaaata ctttttccgg ggggcatttg ttcagcaact ggcgaatggc
      901 cgctggcatg ttctgaggtg tcttcctgaa gcgcgttttg caacagggca tgaccatcag
      961 ggcaggctaa gaaaaaatcg tcttcctgtg gaggtagtga aaatcccg
//
LOCUS       DAJTPG010000297         1001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTPG010000297 DAJTPG010000000
VERSION     DAJTPG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..847
                     /locus_tag="OO230_004284"
     CDS             <1..847
                     /locus_tag="OO230_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5693770.1"
                     /translation="LNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLCV
                     KHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATIL
                     TLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTDA
                     KGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIP
                     GDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQ
                     LLSLK"
BASE COUNT          270 a          254 c          229 g          248 t
ORIGIN      
        1 gttgaatgta gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa
       61 aacgggtgcc gggattgcta ccccgacgat gactactgcg cataaccgcg ctcaggcggt
      121 ttttttatgc gtcaagcaca gccacattca gattatggtg gggcgtgcag ggcagccgca
      181 aggctggccg gtttcggtag tcaccggttg tagcaaccct gtgcgtctca ccacccatga
      241 gattgctacc tctggtggtg agtcatttaa actgactatc gaggctgcca tcatggctac
      301 tatccttacc ctttctcacc ctgacgcaac catcgaaaat ggtcgcgctg tcactacgtc
      361 tgttgcggtt gcggagttct tccggaaaat gcacaaaaac gttattcaga agatcgaaac
      421 tctcgaatgc tcgccggagt ttaaccggct taattttaag cccgtcacct ataccgacgc
      481 caaaggcgaa aaacgcccaa tgtaccaaat caccaaaaac ggcttcgttt tcctggtgat
      541 gggcttcact ggcaaaaaag ccgccgcatt caaggaagcc tacattgctg agttcgaccg
      601 catggaggcc gaactgcgcc agaataacac cccacctgca gacaagatga ttccgggtga
      661 tggtcgcact ctggttgttc acttcgacaa attcggcaat gtcgaattca ccgaaaccgt
      721 tcctgatggc gcactggtct gtaccctgga gactttccgg ttttatctgg aaaaacaggg
      781 atggactctt gttaaccggg gcgcaattaa aaatatgact gtggaacaat tactttcttt
      841 aaaataaaaa tggagctacg gatatgaacc ctatgcttga ttattgtttt acctctgcac
      901 ctcagcagac cataacacac accggaatca aaaatttgtt atggataatc aaaaaaaacc
      961 tccagtagaa aggtgatccc gatttaattc cctgtgctta t
//
LOCUS       DAJTPG010000298          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTPG010000298 DAJTPG010000000
VERSION     DAJTPG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(132..468)
                     /locus_tag="OO230_004285"
                     /pseudo
     CDS             complement(132..468)
                     /locus_tag="OO230_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          329 a          178 c          177 g          306 t
ORIGIN      
        1 cagcacactg ctaaaacgca tagtgatcga gcgctgattc tggcgaacaa ctgaactaat
       61 acatcagaat ctgcattatg ttaaataaat atgaaaagat ggtttaaata ccccgttact
      121 tttgacttac actatacggt atcgcatcgt ttaatattcg caccggccag atttttattt
      181 ctattagttg tcacaatact gaatgcgtac gaccacagta ttctggctcc tgtgtggtta
      241 tgctttaatt ctgcgttccg ggcagataag cagttgcttg caggaatcct tcttgtgtta
      301 atgtcagttc ccctttttac cagtgctgat ttccacattc cgtccaacag agcttatagc
      361 ctttccctgg attatagcat tgtccggctg aagttctttt tgaataataa tagaagcact
      421 gctggcagat ccagtccgtt tttcataacc cactgtactg ataaccataa tctaatcagt
      481 agaaattgag tcgaaaataa gcactactct atacaggata attagaggtc agtttgatta
      541 ttcacaattc atcatcagca ttttctattt ctgacgaaat caacatgaaa ataaccatat
      601 atgataatta ttataataac ggctttaatt ggaatacata tattacaacg tattatatat
      661 aattggtatt ctgggaacta tattctcaaa atacagtaga aacgaggtat gtttctggtg
      721 gaaaggacag tgggattaaa aagtaagaat tgataaaaaa aacgccagca acacatagtg
      781 ccggcggagg gaatacccca tggagaaaat gtgatgccta gagcatcatg aatatataat
      841 taaaaatagt tagcgttgtc aatactttca caaaaataat tttcgtagta tagaaagata
      901 tttttatgca tgacctacct gaatttgctc cgggtagagg ttataaataa aaattgaatc
      961 acgacaaaca caatattcat agtatggcga
//
LOCUS       DAJTPG010000299          979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTPG010000299 DAJTPG010000000
VERSION     DAJTPG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..311)
                     /locus_tag="OO230_004286"
     CDS             complement(<1..311)
                     /locus_tag="OO230_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5693771.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT          312 a          184 c          226 g          257 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa gacgtcgctg atggggcgct tacattaatc ccgtactctc tggccagttg
      421 ggccacgcag gcccctagct ggtaagattt ttcaataaga tcaagcttaa actccataga
      481 gtcatagagt aagaggcacg aatcctgcgg gggcgaagag aatctgacat tgatagagtc
      541 cattaacgtt ataagaggac tctatcatcg ccaatcatca gacattgaaa tagacgacac
      601 tgagatgacg cttacaattc ttttggtttc tgtaataagt aatctgacat cccccgtgcc
      661 cagaagagga gagggatatc aagcccaagg gttaataatt gatactacgg ttaaaattgc
      721 cttcagtaaa ggaaacgtga ttaaattcag gggatttcag agtgaaaacc atacaacata
      781 ttaaaatctg ttcatttagc tataatacat acaagaaagt gtccagttag ataatgttat
      841 ctaaataacc acagataaaa acgcacataa ttgggggggg ggggatgaaa atacctgaag
      901 cagtaaatca tattaatgtt cagaataata ttgatcttgt tgatggaaaa acgaatccga
      961 ataaagcaac aaaagcatt
//
LOCUS       DAJTPG010000300          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTPG010000300 DAJTPG010000000
VERSION     DAJTPG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(3..485)
                     /locus_tag="OO230_004287"
     CDS             complement(3..485)
                     /locus_tag="OO230_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5693772.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO230_004288"
     CDS             complement(476..778)
                     /locus_tag="OO230_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5693773.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          294 a          236 c          195 g          242 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgc
//
LOCUS       DAJTPG010000301          911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTPG010000301 DAJTPG010000000
VERSION     DAJTPG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            29..820
                     /gene="ltrA"
                     /locus_tag="OO230_004289"
                     /pseudo
     CDS             29..820
                     /gene="ltrA"
                     /locus_tag="OO230_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          225 a          212 c          279 g          195 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc gttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tctcggatat gacttcaaag
      841 tgcgtactct gaagaatttc aaaggcgaat tgtacagaaa atgtatgccg ggtgcatcaa
      901 atgcagcaat g
//
LOCUS       DAJTPG010000302          877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTPG010000302 DAJTPG010000000
VERSION     DAJTPG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(41..100)
                     /locus_tag="OO230_004290"
     CDS             complement(41..100)
                     /locus_tag="OO230_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5693774.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..>877)
                     /locus_tag="OO230_004291"
     CDS             complement(339..>877)
                     /locus_tag="OO230_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693775.1"
                     /translation="RAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAE
                     ATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIK
                     IIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSL
                     AALTSPLISTTPVAENVK"
BASE COUNT          190 a          235 c          210 g          242 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgat
//
LOCUS       DAJTPG010000303          858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTPG010000303 DAJTPG010000000
VERSION     DAJTPG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>858)
                     /locus_tag="OO230_004292"
     CDS             complement(<1..>858)
                     /locus_tag="OO230_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5693776.1"
                     /translation="RTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRP
                     AEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVAE
                     DKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRT
                     PVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQP
                     ERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIINSLIQ
                     YDDPAAWTE"
BASE COUNT          209 a          197 c          207 g          245 t
ORIGIN      
        1 gctcagtcca tgctgcagga tcgtcatatt gaataagact atttataata gtgttcgcta
       61 attgttgaac gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcgt
      121 tcagttcttg ctcactgatc cccttagtca aacgcttaac catcacttca ttcgctaacg
      181 ttaactgttc atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt
      241 tagcctgagg atcaaccgag aggcgagaag aaacgctgta tgccccagat gcctgttcac
      301 gaatattaac gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg
      361 tcgccagatt aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag
      421 gttcattttg ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac
      481 ctgcggctaa tggcgaatca gagtgtttga ttgagcctaa gtaacgcgta attaacgcca
      541 cgagtttgtc ttctgcgaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa
      601 acaactggcg atcggcagcc agcgcatctg cggcagtaaa ctgtgcaatc tgattttctt
      661 gcagtaattt cgtgcggtca tcagcatagc gcgtctcata catcagctgg gcgaatttct
      721 ccgctggacg ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc
      781 aaatattgtc gttaatcgtg ctgtgggtga ttcgctggtt aatcaactgg aaaccaggtt
      841 cagggttatt agtccgcg
//
LOCUS       DAJTPG010000304          831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTPG010000304 DAJTPG010000000
VERSION     DAJTPG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          258 a          182 c          174 g          217 t
ORIGIN      
        1 agggaaaccc ataaccaccc gatacgtggg aactaatcgc tttttaactg ttccgcgtgc
       61 gctcagccgc attcaccgca tcacaaaatt cactttaaaa agggcggaca tcagccagca
      121 atgaaactga tgccgccaaa ggtaccaatc aacatggagt gttgtggcgt ggttgtcact
      181 taagcgtatg gtcaacctga caacccggtg tcctcaacgg ggaaggaata actccgccat
      241 acttaccgcc gcgtcatttc gcgttgtgtg cctgctttta actacgtcag gcgaggtggt
      301 tcctgttatt ccccaacaac aagaaatttg tataatctga ataccccaac aataagaaga
      361 gtgagcaagt gatgtattag cgatgtcatc tctcaaagag actgcatcaa tgttaaaatt
      421 attcaatgaa gcaaaaactc aggctgaggt tgatcgcgct gtgtctgaaa taaactctaa
      481 gttgtcaagc attcagcttg aatatttgtc atctcattga tgagctgaca atctctaaaa
      541 gcaatgaggc tgcgctcaaa gcaaaactca cggagataga aaagtttaag ttccaaagtg
      601 aagactgcga acctcgctaa ttgattactg gaacgattgt gtatgccaga cagtgcaata
      661 ctagctctgg caagattacc atgtattttt gtccagaatg ttttgcgaag agagcaatat
      721 ctatattgca acctttccct atcgaggaag gcgataaata tacgaaaact caatgcccct
      781 cctgtaaaaa tgagtatctc atggactaca atttcaataa tgatttttga g
//
LOCUS       DAJTPG010000305          802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTPG010000305 DAJTPG010000000
VERSION     DAJTPG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            206..457
                     /locus_tag="OO230_004293"
     CDS             206..457
                     /locus_tag="OO230_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693777.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            524..802
                     /locus_tag="OO230_004294"
                     /pseudo
     CDS             524..802
                     /locus_tag="OO230_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          215 a          190 c          194 g          203 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg ggtttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctctatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatcgg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aa
//
LOCUS       DAJTPG010000306          784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTPG010000306 DAJTPG010000000
VERSION     DAJTPG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>784
                     /locus_tag="OO230_004295"
     CDS             <1..>784
                     /locus_tag="OO230_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693778.1"
                     /translation="GTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWESLADKF
                     QFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVTGI
                     FPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSETKRHK
                     NGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAELPDYN
                     FSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRV"
BASE COUNT          203 a          169 c          221 g          191 t
ORIGIN      
        1 tcggcacggc atcctacttc gatcctgaag aactctacag tgcgcgagag ttttaccagg
       61 acactatcaa cgctttctac ggcaagcagc agtatctgtt taatccaccg tgggaatctc
      121 tggcagataa attccagttc cgtgaggcag agttgacgct ggtcaatggt gtgcacggtc
      181 acggaaaggc atgcccactg aatgagccta ttcttttagc tgatgggaca tggactactc
      241 acgggaatgt aaaaattggc gatcaggtgg cgtcagtaga cggcaatccg tcaactgtca
      301 ctgggatatt cccgcagggt gttagagatg tttaccgagt cacatttgaa gatggtcgtt
      361 atgttgattg cgcaggcgat cacctatggg aggtcactag tcgtggattc acgaaaggcg
      421 agaaacgccg cgtgatagat accttcgggt tgaagcggtt gagtgaaacg aagaggcaca
      481 aaaatggcgt taggattcct gaaataactg gtgactttgg cgaccactca gagccattag
      541 catgggttat cggctccctt ctcggggatg gtagtcttag caatgggagc gtgaagtttt
      601 caaacgtcga gccatacatg atcgagcgta tgaaggctga actgcctgat tacaacttct
      661 ctggagatgg taaggactgg ctgatatcaa cggcgcgtgg tcaggtaaat ccactcatgg
      721 agaccctgcg aggttatgga ctaatggggt gcacagcaaa aaacaaattc atccctcgtg
      781 tgtt
//
LOCUS       DAJTPG010000307          771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTPG010000307 DAJTPG010000000
VERSION     DAJTPG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..312
                     /locus_tag="OO230_004296"
     CDS             <1..312
                     /locus_tag="OO230_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5693779.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
     gene            315..>771
                     /locus_tag="OO230_004297"
     CDS             315..>771
                     /locus_tag="OO230_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5693780.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPP"
BASE COUNT          134 a          207 c          215 g          215 t
ORIGIN      
        1 gtggctttta tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc
       61 gcatggcaac gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca
      121 ctggcctggg gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc
      181 ggtttgccca tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc
      241 tttgaactgc gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg
      301 caaggagctt aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta
      361 tcggtttgcc tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg
      421 acatgtttga tgtccagatc atggcacaaa cgctggtgaa cggagccgat agcttctccc
      481 tcctggcgat tccgttcttt gtgctggcgg gtgaaatcat gaatgcgggc ggcttgttaa
      541 aacgcattgt tgacctgccg atgaaactgg tggggcataa accaggcggg ctgggctacg
      601 tcggcgtgct ggcagctatg attatggcga gcctttctgg ttctgccgtt gcggataccg
      661 ccgccgttgc cgctctactg gtgccgatga tgcgcagcgc caactacccg gtcaaccggg
      721 cggcggggct gattgcctct ggcggcatta tcgcgccaat tattccccct t
//
LOCUS       DAJTPG010000308          760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTPG010000308 DAJTPG010000000
VERSION     DAJTPG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            56..>760
                     /locus_tag="OO230_004298"
     CDS             56..>760
                     /locus_tag="OO230_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5693781.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPYLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVN"
BASE COUNT          166 a          203 c          225 g          166 t
ORIGIN      
        1 actgcccgga gtgtggcacg gttcactggc cggacggagt cgccccgccc ttctgatgct
       61 cccccgtttt gccgatattt ttcagcaggg aaaccgctgg cttaactggc tggagaaaca
      121 accggaaggg gcagtccgtc cggtggtaat tgaatcagtg acaaaaatca tggcctgcgg
      181 gaccacactg atgggctaca cgcagtggtg ttgttcatca ccggactgtt gccatacaaa
      241 aaaggtctgc ttccggtgta aaagccgctc ctgcccgcac tgcggggtga aggctggcgc
      301 acagtggata cagtatctgc tgagcctggt cccctactgc ccgtggcagc atattgtgtt
      361 cacacttccc tgccagtact ggtccctggt gttccacaac cggtggttac tggcagagat
      421 gagccgcatt gcagcggatg tgatactgga aatctgccat caggcagatg tggagccggg
      481 gatattcacg gtgatccaca catgggggcg tgaccagcag tggcatccgc atatccattt
      541 atcgacaact gccggtggtg tgacgtcggg ccacacctgg aaaaatcttc atttttacgc
      601 ccgtaaggtg atgagcatgt ggcgctaccg gataacacgg ttgctgtcac ggaaataccc
      661 ttacctggtg atgccggatg cgctggcagc agaaggaagc agtaaacggg aatggaatcg
      721 cttcctggac acgcattacc gccgtggctg gaatgtcaac
//
LOCUS       DAJTPG010000309          738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTPG010000309 DAJTPG010000000
VERSION     DAJTPG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(560..>738)
                     /locus_tag="OO230_004299"
     CDS             complement(560..>738)
                     /locus_tag="OO230_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5693782.1"
                     /translation="YKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLLDKSDYVLAHGL
                     NTDDQLAVGIVYQF"
BASE COUNT          202 a          118 c          191 g          227 t
ORIGIN      
        1 cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat tgagtcaata
       61 tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac tggtgttgaa
      121 tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc tcaatgattt
      181 tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg aataattgtg
      241 gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat gcactatttt
      301 tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa agaaatgcat
      361 ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg ggcaggggaa
      421 tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg aaatgccaat
      481 aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt acagtaacac
      541 gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt tggtcgtcgg
      601 tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc tgataagcgg
      661 tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata tagttcagac
      721 gatcagaatc tttgtaac
//
LOCUS       DAJTPG010000310          737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTPG010000310 DAJTPG010000000
VERSION     DAJTPG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            140..569
                     /locus_tag="OO230_004300"
                     /pseudo
     CDS             140..569
                     /locus_tag="OO230_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          183 a          148 c          234 g          172 t
ORIGIN      
        1 cgacaattcc tgccggtatc aacgccgacg gcatgtattg ccctgaatgc ggtacggttc
       61 actggccgga tggcgttatt ccgcctttct gatgcttccc cgttttgccg atatttttca
      121 gcagggaaac cgatggctta actggctgga gaagcagccg gtgcagatga gccgcctgga
      181 acattatgcc ggtcaggatg aaattggcct gcgttacaac agccaccgga caaaacgaga
      241 agaaaacctt gtgatgagtg gcgacgagtt tatggaaagg ttctcctggc atgtggcgga
      301 taaggggttc cggatggtga tacggggtcc tgagtccggt gaagcggcga ttactggaag
      361 atgtggtgta cgccataacg gagacagtga gaaaaacggc gaggcaaatc acaaggagag
      421 ggatgtatca gcagtgtctg aaggttgacc cgctgaagtg cgagctgcgc ggaggtcaga
      481 tgcggtttac ggggctgaag aggggttacc gtctggcaga gcaggttatg atgcatgagc
      541 cgctggctcg aatacggtgg tgcggctgag agtcgcagag gggaagttgc gtccatgttg
      601 aggggaacgg agcaaaaaac catcagagat acattgaatc aatcaatgta agcaatatta
      661 agtgtctata ccgattactt gatgatcagg ttgtgttgaa taaacgcgca ttcttcggtg
      721 cttttttaat cttttgg
//
LOCUS       DAJTPG010000311          735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTPG010000311 DAJTPG010000000
VERSION     DAJTPG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>735
                     /locus_tag="OO230_004301"
     CDS             <1..>735
                     /locus_tag="OO230_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5693783.1"
                     /translation="DQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDY
                     YEPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDR
                     NFEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKC
                     RGWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLY
                     DADRIEEASSTEREVDLYRVGSLTAA"
BASE COUNT          187 a          194 c          209 g          145 t
ORIGIN      
        1 aagatcagga agagtggcaa ggcggctggg tccgcgatgt gaatggcaag atccgtccgc
       61 gtctgggcag caagatgggc gtaataacca aaatcttcgc caacccggtg gtgccgcagc
      121 agattgatga ttactacgaa cctttcacct tcgactacga acatttgcat agcacaccgg
      181 aaggcaaaca tatccctact gctcgcccgc gttcactgat tgacggcaaa cggatggaca
      241 aagtgatctg gggcccaaac tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc
      301 gcgaccgcaa cttcgaggcc atgcaaaagg agatgtacgg gcagtttgaa aacaccttca
      361 tgatgtacct gccgcgcctg tgcgaacact gcctcaatcc cagctgcgtg gcgacctgcc
      421 caagcggcgc tatctacaaa cgcgaagaag acggcattgt gctgattgat caggataaat
      481 gccgtggctg gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac ttcaactgga
      541 aaagcggcaa gtcagaaaaa tgtatcttct gttatccgcg aattgagtca ggccaaccca
      601 ccgtgtgctc agaaacctgc gtgggtcgca tccggtatct gggcgtgctg ctttacgacg
      661 ccgaccgcat cgaggaagcg tcgagcaccg agcgcgaagt tgacctctat cgagtaggca
      721 gcctgacggc tgcgg
//
LOCUS       DAJTPG010000312          720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTPG010000312 DAJTPG010000000
VERSION     DAJTPG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..279)
                     /locus_tag="OO230_004302"
     CDS             complement(<1..279)
                     /locus_tag="OO230_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5693784.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINL"
BASE COUNT          191 a          195 c          149 g          185 t
ORIGIN      
        1 taagttaatt aactcaccac cgaggttttc cacgaccata agggtggtga gacgtacagg
       61 ggtggaaata ccggtcactc gagaacccgg ccagtcttgc gactgccctg cacatcccac
      121 cataatttga atggagctgt gcattacgca taaaaaaacc gcttcagcgc ggctatgcgc
      181 tcgagtaacg ttcgggtttc cacgcccggc ccccgtttta tgaggtgcag atgcactata
      241 attccacccg ttctggtttt caatagctac attcaacatt ttctcttacc tttcatcacc
      301 gaagtgaact ttgttgatgc ggtgcctggt gcctccaggt gacgttaacc agttaacaat
      361 tacagttggc tttcccaccc aaaccaataa ggactaacat gacttttaac tgtgccgcgt
      421 gcgcatagcc gcattcaccg catcacaaaa ttcactttaa aaagggcgga catcagccag
      481 caattaaacc gatgccgcca actggtactt cacacagcaa tgtcgttatt tacaaccgga
      541 agcgcactcc caccatttaa atttcacaga caagaccgac tctttatgga taccggaaat
      601 gcgccttcgt gttgtgcccg gttttatttc accacttccg ggctttggtg gtatctttac
      661 tgaagttctc acacaaccag taaggaaatg aatatgccaa cgtatctcgc cagagtagaa
//
LOCUS       DAJTPG010000313          705 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTPG010000313 DAJTPG010000000
VERSION     DAJTPG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            24..705
                     /locus_tag="OO230_004303"
                     /pseudo
     CDS             24..705
                     /locus_tag="OO230_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          207 a          174 c          184 g          140 t
ORIGIN      
        1 ctcagaacaa cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg
       61 aatccgctca actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg
      121 atgttggcct ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca
      181 aaacaccaaa agcctccccc attaccccgg aacaaattga aatccgtgag ctcaggaaaa
      241 agctacaacg cattgaaatg gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt
      301 cagactccct gaacagttct cgataatcgg gaaactcaga gcgcattatc ctgtggtcac
      361 actctgccat gtgttcgggg ttcatcgcag cagctacaga tactggaaaa accgtcctga
      421 aaaaccagac ggcagacggg ctgtattacg cagtcaggta cttgagctac atggcatcag
      481 ccacggttcg gccggagcaa gaagcatcgc cacaatggca acccggagag gctaccagat
      541 gggacgctgg cttgctggca ggctcatgaa agagctgggg ctggtcagct gtcagcagcc
      601 gactcaccgg tataaacgtg gtggtcatga acatgttgct atccctaact accttgaaag
      661 gcagttcgcc gtgaccgagc caaatcaggt gtggtgcggt gatgt
//
LOCUS       DAJTPG010000314          693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTPG010000314 DAJTPG010000000
VERSION     DAJTPG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            5..>693
                     /locus_tag="OO230_004304"
     CDS             5..>693
                     /locus_tag="OO230_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5693785.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          173 a          162 c          216 g          142 t
ORIGIN      
        1 gaccatgaaa aatattgcga caggaggcgt tctggagcgt atccgcagac tgaccccacc
       61 acatgtaacc gccccattca gaacggttgc ggagtggcgc gagtggcaac ttgctgaagg
      121 ccagaaacgt tgcgaggaga tcaaccgcct gaatcgtcag ttgcgggtgg aaaaaatcct
      181 gaaccgctcc ggcatccagc cgttgcaccg gaagtgttcg tttgcgaatt accgggcgca
      241 gaacgacggt cagcgacatg cactgagtca ggcgaaatcc atagctgacg aactgatgac
      301 aggctgtacg aattttgtgt tcagcggtaa gcccggcacc ggaaaaaatc atcttgcagc
      361 cgcgattggc aaccggctga tggcgaaggg gcgtagcgtg attatcgtca ccgtgtccga
      421 tgtcatgagc gtgttgcatg agagctacga caacggcaaa tccggtgaaa aatttttaca
      481 ggagctttgt ggtgttgacc tgctggtcct ggatgaaatt ggcatgcagc gggatacgaa
      541 aaacgagcag gtggtactga accagattgt tgatcgccgg acggcatcgt tacgcggtgt
      601 ggggatgctg acaaatatta accatgcagc gatgaataca cttctcggcg agcgggtgat
      661 ggatcgcatg gtcatgaacg gcgggcgctg ggt
//
LOCUS       DAJTPG010000315          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTPG010000315 DAJTPG010000000
VERSION     DAJTPG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(135..265)
                     /locus_tag="OO230_004305"
                     /pseudo
     CDS             complement(135..265)
                     /locus_tag="OO230_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(263..358)
                     /locus_tag="OO230_004306"
                     /pseudo
     CDS             complement(263..358)
                     /locus_tag="OO230_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          200 a          125 c          166 g          200 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt
       61 tattttttta cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg
      121 ggtggcgctt ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt
      181 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
      241 ggagggtcag gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg
      301 tcacctgttt atctttcgtg gccgacgggg tgacactgtt aaaatgtaat gaacgcatcc
      361 cacacaatta aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
      421 ctcgcagctt tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg
      481 agactccaca aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga
      541 ttcacttcat caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg
      601 acgatttcct ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct
      661 attgagcttt atttcaaata tggtaagaag c
//
LOCUS       DAJTPG010000316          690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTPG010000316 DAJTPG010000000
VERSION     DAJTPG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>690
                     /locus_tag="OO230_004307"
     CDS             <1..>690
                     /locus_tag="OO230_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5693786.1"
                     /translation="KGNSLKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQE
                     AKAFLGIKDDDHHFDARREKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYE
                     VVSGKVWNGERELDALVLPYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQA
                     LDAGVRVVVLCEGEIDCMSYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFI
                     SMDVDDVGREAA"
BASE COUNT          179 a          140 c          212 g          159 t
ORIGIN      
        1 aagggaaaca gccttaaggt caaccttatc ggcaagaaaa aatgggctga tttcgctgag
       61 ggagacggcg gtgacatgct tgatttgtgg atggcatgtc gtggaattaa cctgcatcag
      121 gctatgcagg aagcgaaagc atttctcggt atcaaggatg acgatcacca tttcgatgcc
      181 agacgtgaga agaaattctc cagacctgat cgtaagaaaa tcgcccgcta cgttaccaga
      241 acagaatccc atcttgagta cctgcaatcg cgtggcatat cgccagaagt cgtaaagcgc
      301 tacgaggttg tcagcggcaa ggtgtggaat ggagaacgag aactggatgc tctggtgctt
      361 ccgtacaaac gcgatggtga gttgttgcag gtcaagcgaa tcagcactga gcgcccggac
      421 gggaagaaag tcattatggc agaaggtgat tgcgaacctt gcctgttcgg atggcaggct
      481 ctggacgctg gcgtgagggt ggttgtgctt tgcgaaggcg aaattgattg tatgagctat
      541 gcgcaatacg gcatctcggc gttatccgtg ccgtttggtg gcgggaaagg cgctaagcaa
      601 cagtggattg agtttgagta tcacaacctc gacaggtttg aggaaatatt catctcgatg
      661 gacgttgatg atgttggtcg tgaagccgca
//
LOCUS       DAJTPG010000317          686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTPG010000317 DAJTPG010000000
VERSION     DAJTPG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            20..289
                     /locus_tag="OO230_004308"
     CDS             20..289
                     /locus_tag="OO230_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693787.1"
                     /translation="MRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWLI
                     GWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            385..594
                     /locus_tag="OO230_004309"
                     /pseudo
     CDS             385..594
                     /locus_tag="OO230_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            510..>686
                     /locus_tag="OO230_004310"
     CDS             510..>686
                     /locus_tag="OO230_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5693788.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRL"
BASE COUNT          215 a          144 c          172 g          155 t
ORIGIN      
        1 aacagatgct gaatacctgg tgcgtaaatt cgatgagctt gaagctaaat gtgcagcaca
       61 ggaaaacaaa gtaataccag tgtcaactga actgccacca gcaaatgaaa gtgttttgtt
      121 attcgatgct aacggagaag gctggctaat tggctggcgt tctctctggt acacctgggg
      181 acaaaaagaa accggagaat ggcagtggac atttcaggtc ggggaccttg aaaacgtcaa
      241 tatcactcac tgggcagtaa tgccaaaagc accggaggct ggagcataat gaccactttt
      301 accgacaaag aactgattaa agaaattaaa gagcgtatca gcagccttga cgtgcgagac
      361 gatattgagc gccgtgctta tgaaatcgca ctcctatctc tggaagtaga accagatgaa
      421 cgcgaagctt atgaattatt catggaaaag cgttttggtg acttagtaga tcgtcggaga
      481 gcaaaaaacg gcgataacga atacatggca tgggatatga ctctcggttg gatcgtctgg
      541 cagcaacgag ctggtatcca tttctcaaca atgtcacagc aagaggtgaa ataatggagc
      601 catacagcct cacactcgat gaggcctgtc attttctcaa gatatccaga ccgactgcca
      661 ttaactggat acgcacaggg cgtctt
//
LOCUS       DAJTPG010000318          686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTPG010000318 DAJTPG010000000
VERSION     DAJTPG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>686
                     /locus_tag="OO230_004311"
     CDS             <1..>686
                     /locus_tag="OO230_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5693789.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKRALFLSA"
BASE COUNT          133 a          236 c          183 g          134 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgggc gttgttcctg tcggcc
//
LOCUS       DAJTPG010000319          682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTPG010000319 DAJTPG010000000
VERSION     DAJTPG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..233
                     /locus_tag="OO230_004312"
     CDS             <1..233
                     /locus_tag="OO230_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5693790.1"
                     /translation="LANIIFVDFQQQGERGLTNAPDEDPDDLSTGYYGSAYRSPENWT
                     TALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          190 a          149 c          163 g          180 t
ORIGIN      
        1 ttttagccaa tattattttc gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg
       61 cgcctgatga agacccggac gatttaagca cgggatatta cggttcagcg taccggtcac
      121 cggagaactg gacgacggca ctgcgaagca gtcatttcag cacggcagcc cgtcggggga
      181 ttatttctga cagatttgta gaagcaattt tgcagttttg gcgcgaaagg tgagcgggga
      241 gtttattcat gggggatgtc cttgttcgtt ctcagagtag acaacaaaaa acaaatgaga
      301 tcaaggtaat atctcttcac ttgcgggctc gaacagcagg caattgccaa tataataatc
      361 tggcacaagg gtgaaaaacg cgcaccgcat aaaccgttgt tattgcttac agattagccg
      421 gatgccttaa tagacatccg cgccttttcg attatggctc ggaaatctac gaacatctac
      481 acagtttact ggaacgtttt gggccacagc gttcgcagca ccgtcctgat atgccgttct
      541 ggcgattata agtcgacgga ttctggcaaa tagacgatga tgatatgctt aatgatgttc
      601 aaagaaatta ggccattatt atattattgc ttgcattttc tttcattcct aaaagcaccc
      661 gccattaatc ccaggcgcat ta
//
LOCUS       DAJTPG010000320          678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTPG010000320 DAJTPG010000000
VERSION     DAJTPG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..678
                     /locus_tag="OO230_004313"
                     /pseudo
     CDS             1..678
                     /locus_tag="OO230_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          146 a          168 c          215 g          149 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatcgt gaggcactgc actgggcggt gactaccggc ggcttcaaca gtgaaacagt
      541 acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgatcttc cgtcgtctcc
      601 agtggagtgg ctgacggata atggttcatg ctaccgggct aatgaaacac gccagttcgc
      661 ccggatgttg ggacttga
//
LOCUS       DAJTPG010000321          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTPG010000321 DAJTPG010000000
VERSION     DAJTPG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            103..>659
                     /locus_tag="OO230_004314"
     CDS             103..>659
                     /locus_tag="OO230_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5693791.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAJTPG010000322          659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTPG010000322 DAJTPG010000000
VERSION     DAJTPG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>659
                     /locus_tag="OO230_004315"
     CDS             <1..>659
                     /locus_tag="OO230_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5693792.1"
                     /translation="SLGCDVSEQLELISSAFKVIETQRPKLACCRCDHIVQAPVPSKP
                     IARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQGVELSRATLGRWTGAVAELLEPL
                     YDILRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYVRDDRNAGSEMPPAVWFAY
                     SPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAACMAHVRRKIHDVHARVP
                     T"
BASE COUNT          143 a          175 c          198 g          143 t
ORIGIN      
        1 tctctgggat gtgatgtgtc agagcagctg gagcttatca gcagcgcctt taaggttatc
       61 gaaacacaac gtccgaaact ggcctgttgc cggtgtgacc atatcgtgca ggcaccggtg
      121 ccttcaaaac ccattgcacg cagttatgcc ggagcggggc ttctggccca tgttgttgct
      181 ggaaaatatg cagactatct gccgttatac cgccagtcag aaatataccg tcgtcagggc
      241 gtggagctga gccgtgccac actggggcgc tggacaggtg ctgttgctga actgctggaa
      301 ccgctgtatg acatcctgcg ccagtatgtg ctgatgcccg gtaaagtcca tgccgatgat
      361 atccccgttc cggtccagga gccgggcagc ggtaaaacaa ggactgcccg gctgtgggtc
      421 tacgttcgtg atgaccgtaa cgccggttcg gaaatgcctc cggcggtctg gttcgcgtac
      481 tcaccggacc ggaaaggtat ccatccacag aatcatctgg ccggttacag cggagtgctt
      541 caggccgatg cttacggtgg ttaccgggcg ttgtacgaat ccggcagaat aacggaagcc
      601 gcatgtatgg ctcatgtccg gagaaaaatc cacgatgtgc atgcaagagt gccaaccga
//
LOCUS       DAJTPG010000323          653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTPG010000323 DAJTPG010000000
VERSION     DAJTPG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..83)
                     /locus_tag="OO230_004316"
     CDS             complement(<1..83)
                     /locus_tag="OO230_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA-like transporter family protein"
                     /protein_id="HCR5693793.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARIT"
     gene            complement(80..598)
                     /gene="mddA"
                     /locus_tag="OO230_004317"
     CDS             complement(80..598)
                     /gene="mddA"
                     /locus_tag="OO230_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5693794.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
BASE COUNT          174 a          183 c          149 g          147 t
ORIGIN      
        1 gtgatgcgcg ccatcagcgt gttctggact accagaccaa ttccggcggc aatcaaaaaa
       61 gcaagggtga gcgactgatt catccaatcg cgtccggttc agtgcgctcg tcgagttgca
      121 actgcataaa tgtcagatcc agccaatgac caaatttagt gcctacctgc ggcatttgtg
      181 cggtgacgac aaatcccagc gactggtgca gatgcagcga agcctgattt tgcgattcga
      241 tcccggcgac catgacatgc ttcccgcaat cccgcgcttc atcaatcaat cgacttaaca
      301 atttacgccc cagacctttg ccctgatgat cgggatggac ataaaccgaa tgttccacgg
      361 tatggcgaaa accatcgaaa ctacgccagt cgccaaacga ggcatatccc gtaactacgc
      421 catcttcttc acttaccagc accggataac ctgctatggt ccgcgcttca aaccaggcaa
      481 tgcggttatc tgcatccacc gtttggtcat tccagatagc cgccgtatac aacacggcgt
      541 ggttataaat ttccgcaatg gcagcacagt cggctttgcg ggcaaaacgg atggacatgt
      601 ctggcctcaa taaaataatg atgatgatgt tatcaatact atcgaaaaca gcc
//
LOCUS       DAJTPG010000324          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTPG010000324 DAJTPG010000000
VERSION     DAJTPG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(24..>649)
                     /locus_tag="OO230_004318"
     rRNA            complement(24..>649)
                     /locus_tag="OO230_004318"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAJTPG010000325          646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTPG010000325 DAJTPG010000000
VERSION     DAJTPG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>646)
                     /locus_tag="OO230_004319"
     rRNA            complement(<1..>646)
                     /locus_tag="OO230_004319"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          214 c          145 g          166 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcagaccag ctagggatcg tcgcctaggt gagccgttac cccacctact
      541 agctaatccc atctgggcac atccgatggc aagaggcccg aaggtccccc tctttggtct
      601 tgcgacgtta tgcggtatta gctaccgttt ccagtagtta tccccc
//
LOCUS       DAJTPG010000326          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTPG010000326 DAJTPG010000000
VERSION     DAJTPG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..209)
                     /locus_tag="OO230_004320"
     CDS             complement(<1..209)
                     /locus_tag="OO230_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5693795.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALS"
     gene            complement(206..616)
                     /locus_tag="OO230_004321"
     CDS             complement(206..616)
                     /locus_tag="OO230_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HCR5693796.1"
                     /translation="MGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMY
                     DVVTNAAGNSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTA
                     LNMFTSASNAGYGKEDDSAVIKIFSGITLPGAKS"
BASE COUNT          147 a          176 c          158 g          154 t
ORIGIN      
        1 cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga gcgcgttttc
       61 aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac accgttaatt
      121 tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc gccggtaaaa
      181 tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg atgccagaga
      241 aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg gcgctggtga
      301 acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg gctgtatcgg
      361 caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg taatcgccat
      421 ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca ttggtcacga
      481 cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc gcttcggctc
      541 cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc gaacctagtc
      601 ccggttctgc acctatgcga taaacttttc cggca
//
LOCUS       DAJTPG010000327          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTPG010000327 DAJTPG010000000
VERSION     DAJTPG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            18..491
                     /locus_tag="OO230_004322"
     CDS             18..491
                     /locus_tag="OO230_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5693797.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTPG010000328          628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTPG010000328 DAJTPG010000000
VERSION     DAJTPG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>628
                     /locus_tag="OO230_004323"
     CDS             <1..>628
                     /locus_tag="OO230_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5693798.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTT"
BASE COUNT          197 a          113 c          152 g          166 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacg
//
LOCUS       DAJTPG010000329          606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTPG010000329 DAJTPG010000000
VERSION     DAJTPG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            61..360
                     /locus_tag="OO230_004324"
                     /pseudo
     CDS             61..360
                     /locus_tag="OO230_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
     gene            complement(468..>606)
                     /locus_tag="OO230_004325"
     CDS             complement(468..>606)
                     /locus_tag="OO230_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693799.1"
                     /translation="FTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTTTKMLFMCR
                     E"
BASE COUNT          195 a          144 c          111 g          156 t
ORIGIN      
        1 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
       61 catagcagcg ctcagtactc cggcgaaata aagggatacg attacgatac atataccttc
      121 tatgccaaaa aaggtcagaa agtgcatgtg agtatctcta atgaaggcgc tgatacttat
      181 ctcttcgggc caggaatcga tgattctgtt gatttatcca gatattcccc agaactcgat
      241 agtcacggac aatactcact ccctgcttct ggcaaatatg agctgcgagt gctgcaaact
      301 cgaaacgacg cccgtaaaaa caaaactaaa aaatataacg tcgatattca aattaagtaa
      361 atgtcaaccg acggggacta tccccgtcat agacgttaac gccccactcc tctctgtcgc
      421 tcaagaggtt aatgacgaat agcagagaac ataatgtaaa caaggcatca ctcacgacac
      481 ataaataaca tctttgtggt ggtttgattg atttttccac tccgcttacc atctacacca
      541 aaataacgat catcacgaca taatttttct ttaacatcgc aaaatacacc gttagcgaat
      601 gtaaat
//
LOCUS       DAJTPG010000330          601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTPG010000330 DAJTPG010000000
VERSION     DAJTPG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..580)
                     /gene="aqpZ"
                     /locus_tag="OO230_004326"
     CDS             complement(<1..580)
                     /gene="aqpZ"
                     /locus_tag="OO230_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5693800.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAV"
BASE COUNT          191 a          176 c          132 g          102 t
ORIGIN      
        1 caaccgcggt gctgcgcgcc gggttaacag aagtgttagt caccggaata ctgattaagt
       61 gaatcagggt taaggccaga ccaatagcga tcggcgcaaa acctgccgga gcgaatttgt
      121 cggttgcacc gtggatcact aacaggaagc ccgcactcaa taccagttca actaccagcg
      181 cggaaagcat ggaataaccg cctggtgaat gctcgccata accgttagaa gcaaaaccgc
      241 tggctgccgc gtcaaaaccc gttttaccac tggcaattaa atacagcagc gccgctgcaa
      301 caataccgcc gacaacctgg gcaattacgt agccaacgac ttcttttgcc ggaaaacgtc
      361 cgccagccca taaaccaata gtgaccgccg ggttaaaatg accaccagaa atatgaccaa
      421 cagcgaaggc catcgtcaga acggtcaaac caaatgccag cgccacgccg gcaaaaccaa
      481 tgcctaattc cgggaagcct gcggccagta cagcactacc acagccacca aaaacaagcc
      541 agaaagtacc aaaacattca gctgctaatt ttctgaacat atccacctca attaaaaatt
      601 g
//
LOCUS       DAJTPG010000331          600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTPG010000331 DAJTPG010000000
VERSION     DAJTPG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(244..>600)
                     /locus_tag="OO230_004327"
     CDS             complement(244..>600)
                     /locus_tag="OO230_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5693801.1"
                     /translation="SICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQ
                     AVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTR
                     SEQKAAFSKQSIAKVD"
BASE COUNT          152 a          156 c          112 g          180 t
ORIGIN      
        1 ttgctggact tgaaccagcg acctggcgat tatgagtcgc tcgctctcac cactgagcta
       61 aagggccgga ggcagaataa taaccatatg tcatcacatc tgcaaactca tctgaccacc
      121 agcacgttta acgtcctgtg ccatttttca ggcataaaaa aaccgctcgg cggcgggttt
      181 tgcttacttt accatcgcgt acaaaatcgg caaagtatca gatttgcatg agatatatgc
      241 cttttaatct acttttgcaa tactttgctt tgaaaatgcc gctttttgtt ctgaacgtgt
      301 tctcatcaca aacaataaag cctcactatc cagtcggtga aaaatgtgtt tcattacaac
      361 ccagtgacca gtaaatgttt tggaccagtt tttggttgtt actcccgcca gtaatgccag
      421 ctcctgatat tcataacctt ccccaccaaa aagttctgct tttaccgcct gcgccgccag
      481 ccagattaat tttttcaggc gttcctgtgt tttctctgca attttcctag tgccagaatg
      541 agccttaaac tcttcccacg cccagtgggt tatggtgatc tgatactccc agcaaatact
//
LOCUS       DAJTPG010000332          597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTPG010000332 DAJTPG010000000
VERSION     DAJTPG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          149 a          150 c          140 g          158 t
ORIGIN      
        1 cattcctttt aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc
       61 gactttaagt tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt
      121 actgaggtct gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg
      181 tctgaggttg gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg
      241 ttatggattg tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg
      301 ccaacccgaa gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttactgg
      361 aagtactgaa cactttcgcg tcaagttctc cgaagttcat tcctgtttga ctaaactatc
      421 actataggga ccatatctcg cctctgacct cctgagagac ggcaagcgag aaaccgtggt
      481 ccacgctagc aagctagcca ccagcctaag gcaagacagc ctctggtcag cctgtgtgtt
      541 ctttggtctc gtgacttaag tctatcacca cacacgctcg gacatgctgt cgaacct
//
LOCUS       DAJTPG010000333          597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTPG010000333 DAJTPG010000000
VERSION     DAJTPG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(151..309)
                     /locus_tag="OO230_004328"
     CDS             complement(151..309)
                     /locus_tag="OO230_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5693802.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(380..>597)
                     /locus_tag="OO230_004329"
     CDS             complement(380..>597)
                     /locus_tag="OO230_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HCR5693803.1"
                     /translation="SRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLG
                     VQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          139 a          157 c          164 g          137 t
ORIGIN      
        1 tgctccttat cggtggttac gatttactgg tgtaataaat gaaaaagctg cgtctcccgg
       61 agaagacgcg gcctgacaga tgaaatgaat gacgtttatt gtctgagaag cccttaactg
      121 gcgagctgag tattaagctg tgttccggca tcaccagcgc aactgacctt cagcattacg
      181 gataaccagc cgggaatatg ttccctggtc atcttcagta aacacattgc ggtaagctgt
      241 tttgacggca acagcctgtt cgcgtgagaa agggccttcg ggcaataaac gtgatgtaca
      301 acccggcata tctgttgctc cctgaaagta aaaagccccg gtcatgatga ccggggcctg
      361 aaggagagtg acctgattat caaaaagtca cattcagcgt ggcctgaccg ttataacctt
      421 cagcgctgct gccgctgacg ctgtgggcat aaccggcctg aacgcccagg gtgatatttt
      481 cccggacacg ggcttccagt ccggcctgca ggtccagtga cgtgccattc cgggacggtg
      541 agaacgtcat gttactgccg gcggcggctg tccccatgct catgtcaccc cgggagc
//
LOCUS       DAJTPG010000334          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTPG010000334 DAJTPG010000000
VERSION     DAJTPG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          158 a          121 c          125 g          190 t
ORIGIN      
        1 atggcttcac ccgtgttaat atcctggata actgtgtttg aaggatttct gttaataatc
       61 tgctcctgca actcacctga tgagtattag tggtaataca taatcttact cgtaaaagag
      121 tataccttat gtcattaaac agtcttccgg cgtgttgttc aatcctgtac ctctgttact
      181 tatctggtac gttgatatgt gtaatgctac gttaattgca atatctggtt atggctttat
      241 accttcagca tttaaaatat tttattaccg gagaaacagg gaagtggaaa gacttttggg
      301 ataatgtcag gccattctga cgttaatcac agtaccaaat aacgagaggg taatggaagt
      361 taaaacctgc gtaagttcct ctgttgttca ctggaaaaag tgattgccca cacgcgttat
      421 aaaggccttt ccgctgcagc ctggttaaca gagcttcagg cgttatatgc gctgtatggc
      481 tgttacgtat gctgttcttt gcctggttct gatacccgtg gaagtcgtta ccggaggaaa
      541 cacgttcacc ctactacctg agaacatctt cttcaaaaat tacggcaacg cttc
//
LOCUS       DAJTPG010000335          587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTPG010000335 DAJTPG010000000
VERSION     DAJTPG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..530
                     /locus_tag="OO230_004330"
     CDS             <1..530
                     /locus_tag="OO230_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5693804.1"
                     /translation="RIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNARTSD
                     ISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITYDA
                     YHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKAYV
                     AEAISVLAPFFEETL"
BASE COUNT          162 a          141 c          148 g          136 t
ORIGIN      
        1 gccgcatcaa ggccatcggt accgttagtg cggtcaatat tggttcgatg ttccgcaatg
       61 gttgggaaaa caatgtgaaa tctattgatg ctctgccaca cattgaagct ggctcaaacg
      121 cccgaaccag cgatattagc agcgaagaat acgccattat gccactggct ccaatgaagg
      181 aggccgatgc gccgaacgaa gaactgcgtc aggcctggga gtattaccac acacctcgtg
      241 cgcagtatcc aacagcaccg ggttacgcta ctttgcgcag ccttaaccag attattacct
      301 atgatgctta ccatatggcg gaagtgtacc tgactcagcc gatgcagatt gtggcgggca
      361 gccaggcagg gagcaaatgg atgagtgacg acctgtacga tcgggcatca agccaggata
      421 aacgctatca cattgttgaa ggcgcaaacc atatggatct ttatgacggc aaagcctatg
      481 tggctgaagc aatttcagta ttagctccgt tctttgagga aactctgtaa tgaaacatat
      541 tcaaatccgc aatagtgata tggcctggca tattgctgcc aatattc
//
LOCUS       DAJTPG010000336          585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTPG010000336 DAJTPG010000000
VERSION     DAJTPG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>585)
                     /locus_tag="OO230_004331"
     CDS             complement(<1..>585)
                     /locus_tag="OO230_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5693805.1"
                     /translation="QTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIILLNSHDGTSS
                     YQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFDRIEEKRDAM
                     QSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENM
                     LKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT          111 a          174 c          159 g          141 t
ORIGIN      
        1 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
       61 atacgttttc cttttgcact gcggccggaa atcccgcctt tcagcatatt ctcctggatg
      121 gtctgatatg cgctccacag gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg
      181 tcagcggtgg tgactggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
      241 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttttcctca
      301 atccggtcaa aaacccccac cacttcgtaa gccccttcga taactttctc caccacattt
      361 ccccggtgtg gaacacgcac ttcccccaga gactgaccgc agacgcagcc attctggcag
      421 acgaatctga agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc
      481 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
      541 tgttttgtgt atccccggcg gcccgggtcg cgcacgcggg tctgg
//
LOCUS       DAJTPG010000337          579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTPG010000337 DAJTPG010000000
VERSION     DAJTPG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(6..>579)
                     /locus_tag="OO230_004332"
     CDS             complement(6..>579)
                     /locus_tag="OO230_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCR5693806.1"
                     /translation="EAGAFVLMSLESGPLMTMIILGTAGIASFEPHVFVGAVLPFLVG
                     FALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGLLGILLGVAVIIVTGI
                     PLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEMVPAFKPMAPAATSLVATAVIV
                     TSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT          163 a          168 c          133 g          115 t
ORIGIN      
        1 caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt
       61 gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc
      121 agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca
      181 ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca
      241 ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat tactgccaca
      301 cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc
      361 agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat
      421 tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa
      481 acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc
      541 ccggactcca acgacatcaa cacaaatgcc ccagcttct
//
LOCUS       DAJTPG010000338          576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTPG010000338 DAJTPG010000000
VERSION     DAJTPG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>576)
                     /locus_tag="OO230_004333"
     CDS             complement(<1..>576)
                     /locus_tag="OO230_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5693807.1"
                     /translation="GTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVT
                     DVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVL
                     HSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNI
                     SELKDAVTEYIEYYNSRRISLKLKGLTPIEY"
BASE COUNT          154 a          131 c          108 g          183 t
ORIGIN      
        1 gatattcaat tggagtcaga ccttttaatt tcaggctaat tcttctgctg ttatagtatt
       61 caatatattc cgtaacagca tccttcagtt cgcttatatt actgaactca tcaagataaa
      121 aacactccga ctttaaggtt ccaaagaaac actccaccac agcattatcc agacaattgc
      181 cttttctgga catgctttgt ttaataccat gttctttaag gatattttga tatcttctca
      241 tacgatactg ccatccctgg tcagagtgca gaacaggatg ctcgtgagga ttaagctttt
      301 tgaatgcctg atcgagcata ttctcaacca tgttcatcac tggtctttcc gaaaggctgt
      361 aagaaataac ttcgttgttg aagagatcta ttactggaga caaatacagc ttgcgcccat
      421 tgactgcaaa ttcagtaaca tcggtaaccc acttctcgtt tggccgcgta gccttgaaat
      481 ctctttggag aacattaggg gcggtttgcc ctacctctcc tctgtaagag cggtatcgct
      541 tgaccttaat cgctgcttta agtgagaggg ttccca
//
LOCUS       DAJTPG010000339          569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTPG010000339 DAJTPG010000000
VERSION     DAJTPG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(255..>569)
                     /locus_tag="OO230_004334"
     CDS             complement(255..>569)
                     /locus_tag="OO230_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5693808.1"
                     /translation="YGLPAQKLMTALLQHRGLFRKNPISNEWSVNPKYSDCFRIIKPS
                     DQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIAFTGTDQQKQEHLLKLAQSGKVE
                     GI"
BASE COUNT          126 a          145 c          154 g          144 t
ORIGIN      
        1 cacagtaagg cctacgggtc tccccgcatc ggtcatctca aaaggtgttt aaattaggta
       61 ttgagtaggt gagctactaa cggttaagcg gcagtttcat agcgaccagt tgggctatta
      121 gctcgtctcg ggattggtgc tggcgggtct catcttccgg gtctagacca agctctctga
      181 ggaggacttc agtccggtag ccacggtaac ggagagtcat gcgcccctca ggtgtgcgcg
      241 ggtcaatcat catcttagat tccctccacc ttaccggatt gtgccagctt caggaggtgc
      301 tcttgcttct gctggtcggt gccagtgaat gcaataagga tgcctcgcat cttgtcaacc
      361 atctcaggct taccgaagac ctccagcccc ttagcgttga accggaagtt atacccacct
      421 gctgagaact tctggtctga cggcttaatg atacgaaagc agtcggaata cttcggattt
      481 acagaccact cgttactgat tgggttctta cgaaagaggc cacgatgctg gagaagtgcg
      541 gtcattagct tctgagcggg cagaccata
//
LOCUS       DAJTPG010000340          569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTPG010000340 DAJTPG010000000
VERSION     DAJTPG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            39..>569
                     /locus_tag="OO230_004335"
     CDS             39..>569
                     /locus_tag="OO230_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5693809.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKL"
BASE COUNT          173 a          146 c          140 g          110 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacccga
      361 tcgccagtaa gcctgaatcc ccgggacgaa aatcttcacg aaaaccgctg ccggcagaac
      421 ttccccggga gacacatcgc cttctgcctg ctgaaaccag ttgcccggcc tgtggaggtg
      481 ttctgaaaga aatgggggaa acaatctcag agcaactgga tatcattaat accgccttta
      541 aagttatcga aaccatacgt cccaaactg
//
LOCUS       DAJTPG010000341          552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTPG010000341 DAJTPG010000000
VERSION     DAJTPG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..552
                     /locus_tag="OO230_004336"
     CDS             <1..552
                     /locus_tag="OO230_004336"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693810.1"
                     /translation="GDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQ
                     AIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGL
                     TGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNE
                     VETALFNARALALKIYSMSGWNL"
BASE COUNT          164 a          104 c          135 g          149 t
ORIGIN      
        1 ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag aactcagcat
       61 gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga tggtgtgaca
      121 gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc ctccggcggg
      181 gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc cactttgctt
      241 attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg agcaaaaagc
      301 ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg ccgttatctg
      361 agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa cattacagca
      421 gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat ggtgaatgaa
      481 gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag catgtcaggc
      541 tggaacctgt ag
//
LOCUS       DAJTPG010000342          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTPG010000342 DAJTPG010000000
VERSION     DAJTPG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..151
                     /locus_tag="OO230_004337"
     CDS             <1..151
                     /locus_tag="OO230_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5693811.1"
                     /translation="ALFDEVASASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITP
                     DSEDE"
     gene            complement(153..379)
                     /locus_tag="OO230_004338"
                     /pseudo
     CDS             complement(153..379)
                     /locus_tag="OO230_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          140 a          140 c          121 g          150 t
ORIGIN      
        1 cgcgttgttc gatgaagtgg cctctgccag cctcgcaccg atctctgcat ttgacgttct
       61 gattagcgat cgtccgttac cggcagatta tgttacgcac tgccagaatg gttctgtaaa
      121 gatcattaca cctgattcag aagacgaatg acttactgaa aaaacaccac aatcttgtta
      181 aacatcgtcg ggttggactg attacgttgc actttcacca catattccag cttatctatt
      241 tggcttatca cctactccag acgctggtca tccttgacca gtagccagat atggcttttg
      301 tcgcagtcct gaatcggcag gcagagaatg tcttcaacgt taagagcgcg acgggcaaaa
      361 gcccacaaag gtgagtcatt acacccggat ggtaaacagg cacttccttg ttgtagaagc
      421 cgttatattg tccataatgt ctgggttttt aatgcatact gggtaacaac cggacgaatc
      481 ttcgaatatc actggcgctg ccagccggtt tgaattcatc tcacaaccct gcatagagcg
      541 aatctcctgc c
//
LOCUS       DAJTPG010000343          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTPG010000343 DAJTPG010000000
VERSION     DAJTPG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..490)
                     /locus_tag="OO230_004339"
     CDS             complement(<1..490)
                     /locus_tag="OO230_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5693812.1"
                     /translation="MRQRELAATAKAFMLDRPEVSLQELTEHLRSMAEQFLTDASDDY
                     WNGLEVATLVDEKSNLKELAATQALSLDQQKGIRLALEVLTAAQQRVDTGDTSGLIKL
                     IDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQERQSSVVFSSLVSSLLAEKVQTLKTS
                     SYK"
BASE COUNT          119 a          146 c          138 g          145 t
ORIGIN      
        1 ccttgtagct ggatgtcttc agggtctgga ccttctcagc cagcagcgag gacactaggc
       61 ttgaaaaaac tacagatgac tggcgctcct gtgtgaaaac tgccggtcta tccccctgct
      121 cattattcag aattgacgta gagtcgccta tagttgagtc atcagtaagg ttattatcat
      181 caatcagctt aatcagacca gaggtatcac cagtgtcgac tctctgctgt gctgcggtca
      241 ggacttccag cgctaacctt atgcccttct gctggtccag actcaaggct tgcgtagcgg
      301 ctagctcctt caggttggac ttctcgtcga ccagagtagc gacctcaagt ccattccagt
      361 aatcgtcact agcgtctgtc aggaactgct cagccataga ccggagatgc tctgtaagct
      421 cctgtagtga gacttcaggc ctatccaaca tgaaggcctt agcggtggct gcaagctctc
      481 tctgacgcat tgtggctact ctcctgtctg tgctgcgggt cgatactgaa agaaacggtg
      541 tggtctgt
//
LOCUS       DAJTPG010000344          540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTPG010000344 DAJTPG010000000
VERSION     DAJTPG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>540
                     /locus_tag="OO230_004340"
     CDS             <1..>540
                     /locus_tag="OO230_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5693813.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKA"
BASE COUNT          117 a          144 c          167 g          112 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
//
LOCUS       DAJTPG010000345          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTPG010000345 DAJTPG010000000
VERSION     DAJTPG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>538
                     /gene="tuf"
                     /locus_tag="OO230_004341"
     CDS             <1..>538
                     /gene="tuf"
                     /locus_tag="OO230_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5693814.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTC"
BASE COUNT          122 a          133 c          157 g          126 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgta
//
LOCUS       DAJTPG010000346          538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTPG010000346 DAJTPG010000000
VERSION     DAJTPG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..502
                     /locus_tag="OO230_004342"
     CDS             <1..502
                     /locus_tag="OO230_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR5693815.1"
                     /translation="AHKLGSQFHIPHGLANALLICNVIRYNANDNPTKQTAFSQYDRP
                     QARRRYAEIADHLGLSAPGDRTAAKIEKLLAWLETLKAELGIPKSIREAGVQEADFLA
                     NVDKLSEDAFDDQCTGANPRYPLISELKQILLDTYYGRDYVEDETAAKKEAAPAKAEK
                     KAKKSA"
BASE COUNT          144 a          145 c          138 g          111 t
ORIGIN      
        1 ggcgcacaaa ctgggttccc agttccatat tccgcacggt ctggcaaacg ccctgctgat
       61 ttgtaacgtt attcgctaca atgcgaacga caacccgacc aagcagactg cattcagcca
      121 gtatgaccgt ccgcaggctc gccgtcgtta tgctgaaatt gccgaccacc tgggtctgag
      181 cgcaccgggc gaccgtactg ctgctaagat cgagaaactg ctggcatggc tggaaacgct
      241 gaaagctgaa ctgggtattc cgaaatctat ccgtgaagct ggcgttcagg aagcagactt
      301 cctggcgaac gtggataaac tgtctgaaga tgcattcgat gaccagtgca ccggcgctaa
      361 cccgcgttac ccgctgatct ccgagctgaa acagattctg ctggatacct actacggtcg
      421 tgattatgta gaagatgaaa ctgcagcgaa aaaagaagcc gctccggcta aagctgagaa
      481 aaaagcgaaa aaatccgctt aatcagtagc gctgtctggc aatataaacg gccccttc
//
LOCUS       DAJTPG010000347          536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTPG010000347 DAJTPG010000000
VERSION     DAJTPG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>536)
                     /locus_tag="OO230_004343"
     CDS             complement(<1..>536)
                     /locus_tag="OO230_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5693816.1"
                     /translation="AVAAFYALFVATVIYREMTFATLWHVLIGAAKTTSVVMFLVASA
                     QVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTPTVLILTPVL
                     MPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDAVRGVFPYVL
                     VLYSLLVVFVFIPDLIIL"
BASE COUNT          186 a          117 c          125 g          108 t
ORIGIN      
        1 ggaggatgat gagatcggga ataaaaacaa atacgactaa taatgaatat aaaaccagga
       61 cgtaagggaa aacgcctctg actgcatcat cgaatttgag ttttgccacc cctgaaataa
      121 cgttaagtac attaccgata ggcggtgtaa ttaagccgat tgagcagtta atgataaaca
      181 tgacaccgaa ataaatcgga tcgattcctg cctctttaac taaaggcatt aataccgggg
      241 taagaattaa taccgtcggc gttaaatcca tgaccatgcc gacaatcagg atagccacca
      301 taatgacgat aaacagcaga cgcggtgaat cgaccaacgg ttgcagtaaa tcagaaacca
      361 tcatcggcag ttcagcaatg gtaatcagcc aggcggaaac ttgtgccgag gccaccagaa
      421 acataaccac tgaggtggtt ttcgccgcgc caatgagtac atgccaaagt gtggcgaagg
      481 tcatttcacg gtaaataact gtggcgacaa acagcgcata gaaagcagca acggcc
//
LOCUS       DAJTPG010000348          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTPG010000348 DAJTPG010000000
VERSION     DAJTPG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>533)
                     /locus_tag="OO230_004344"
     CDS             complement(<1..>533)
                     /locus_tag="OO230_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR5693817.1"
                     /translation="AKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKNTDA
                     VLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKTATF
                     DFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQIVQ
                     EGSLEYATDVIIHLQQI"
BASE COUNT          128 a          113 c          134 g          158 t
ORIGIN      
        1 tcaatctgtt gaaggtgtat tattacatca gtagcatatt ccagactgcc ttcctggacg
       61 atctgatcca taacatcctc acgcacttta tatcttgtgc atttcatttc aagtgccgtg
      121 agtacagcca ttactggtga tggtagctgt gttgttttca ggccgtcgat ctgactgatc
      181 gcttgggcga cggtgttttg ccattctaaa gaacaggtgc tgaagtcaaa tgtggctgtt
      241 ttattatccc gttgcgacac gctacaggct aagcgacgta attgggtcaa tttgtctgct
      301 tgctgaacag gaactccggg aaagcggcga tgacttaacg tggtgtgtac aacttcgggc
      361 ggaaaaacga cagcaatatc tttcgccagc caaggtaaaa cagcatctgt atttttgtca
      421 gccgcacggg tttctgctac gaaagagggt tgttcctgaa taatggtgga taagtccgct
      481 accttgagag agtccgttac cgtttgggaa ggttgcaaag aattatcttt cgc
//
LOCUS       DAJTPG010000349          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTPG010000349 DAJTPG010000000
VERSION     DAJTPG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          173 a           78 c          107 g          175 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc
      361 tcttattttc cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga
      421 tctcatttcg agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg
      481 attggggttt tatgagaaat aaagagacgt attgtctttg tttaaacggt acg
//
LOCUS       DAJTPG010000350          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTPG010000350 DAJTPG010000000
VERSION     DAJTPG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..366)
                     /locus_tag="OO230_004345"
     CDS             complement(<1..366)
                     /locus_tag="OO230_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693818.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQ"
BASE COUNT          165 a          116 c           93 g          158 t
ORIGIN      
        1 ctggcctctt ggcatcttga acgcaaatgt aaaaccaaca gtatctgaat caaaatcatc
       61 atctttatat attgggatca aaaatgtcat cgtcgctggt gatgcattgt ttgcaaaatc
      121 aaatacacct atacttttgc cgaattcata agaagctttt ctcaatataa tcgcaccgct
      181 ggcagaaggg gatgggcacg atatcctcag ttgtctgaat ccatctgtat ccaagtcaac
      241 gtcattatct gcagatgttt taacgttact aataaatcgt gaaacaatct cctcatttgt
      301 tagtattttc gcctgagcac agcaagaggt taatattgca accgtcatta aaaatggctt
      361 tatcacaaaa caaactccat tgttagcatg ttacgcgata attttgcctc atctttcacc
      421 acaaaaaaag aactcacctt ttaaatggag aatacccaac atcatgggtg attttcataa
      481 agggatcgcc tgttttcggc aaatcgataa cgcgcattcc aggcggcgcg tt
//
LOCUS       DAJTPG010000351          526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTPG010000351 DAJTPG010000000
VERSION     DAJTPG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>526
                     /gene="ospC2"
                     /locus_tag="OO230_004346"
     CDS             <1..>526
                     /gene="ospC2"
                     /locus_tag="OO230_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5693819.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFISNGLVDVNK"
BASE COUNT          200 a           63 c           96 g          167 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattag taatggattg gttgatgtaa acaaaa
//
LOCUS       DAJTPG010000352          514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTPG010000352 DAJTPG010000000
VERSION     DAJTPG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(25..498)
                     /locus_tag="OO230_004347"
     CDS             complement(25..498)
                     /locus_tag="OO230_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5693820.1"
                     /translation="MAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAEL
                     GDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSP
                     GSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT          133 a          128 c          108 g          145 t
ORIGIN      
        1 taatattttt taaatgcaac attgttaaat actcgctacg ttataccagg tgccattaat
       61 cagtttttgc agtggtcggt aatacacacc gtcaacgtta tccgctgagt tacggccggt
      121 atccgatatc gccatccctg acagcccgtg tcccgaaggt gcgcaaaatc tccacgaaac
      181 ggtgttactg ccggggctgt aatacatttc atggccatac cgtacatcct gtaccccctc
      241 tgttcgatat ttatagcggg catcgaagtt tccgtaattt aacggaatta cctgtccgtt
      301 aacagtgaac actatgctgt tatccgggtt tctctggctg aaaaaatgcc agcctgaatc
      361 atcaccaagc tctgcaacaa caggcctgga tggattaccc cacaaataaa acgctgcatt
      421 tttcgtggag ttgttggcgc tggataacgt gaattttctg gcagttccgg cctgaatatt
      481 cttaaaagca atagccactc cattctgaaa gcgg
//
LOCUS       DAJTPG010000353          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTPG010000353 DAJTPG010000000
VERSION     DAJTPG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..330
                     /locus_tag="OO230_004348"
     CDS             <1..330
                     /locus_tag="OO230_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5693821.1"
                     /translation="KHAEMAKAFDYILNHWNALNEFCRDGRVEIDNNIGENALRSVAV
                     GRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLP
                     WNFSSVK"
BASE COUNT          138 a          114 c          146 g          113 t
ORIGIN      
        1 aaacatgcgg agatggcgaa ggcgttcgac tatatcctga atcactggaa tgcgctgaac
       61 gagttctgtc gtgacggccg ggtggaaata gacaacaaca tcggtgaaaa cgcgttacga
      121 tcggtggcgg ttggaagaaa aaattatctc tttttcggct cagacaaggg aggagaaagt
      181 gcggcgatca tctacagtct gctggtcacc tgcaaacaga acgaagtgga gccggaggac
      241 tggttgcgcg aagtgatcga gaagctcaat gactggccgt cgaaccaagt gcatgaactg
      301 ctgccctgga acttctcgtc tgtaaaataa tccttacgct acgtacttct cggggcgctt
      361 accatacggt ggtcggttta cgcttaccgc aggacaggtg gtaactgaaa aatagtttgc
      421 tgaggcgact ggaaacagaa gggcaagatc gattccgaca gcagtgatgg tcagctacct
      481 catcgataac tttgcaacag tgcccttctt g
//
LOCUS       DAJTPG010000354          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTPG010000354 DAJTPG010000000
VERSION     DAJTPG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..511
                     /locus_tag="OO230_004349"
                     /pseudo
     CDS             1..511
                     /locus_tag="OO230_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAJTPG010000355          505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTPG010000355 DAJTPG010000000
VERSION     DAJTPG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>505
                     /gene="ospC4"
                     /locus_tag="OO230_004350"
     CDS             <1..>505
                     /gene="ospC4"
                     /locus_tag="OO230_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5693822.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYK
                     VLEYFINN"
BASE COUNT          199 a           60 c           87 g          159 t
ORIGIN      
        1 aaaactgatg ttccaatatt taacactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa ggattcagag agttttgtta taacaaaaat
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagtacaga taacttcaaa aaaataaaac tcagagacat atctttggag
      241 gatgcgatta aagcatctaa ttatgaagag attaacaata aagtgactga taaaaaaatg
      301 gctcatcagg cccttgctta ttctttaggc aataaaaaag ctgatatagc actttattta
      361 ctatctaaat ttaattttac aaaacaagat gtcgcagaga tggagaaaat gaaaaacaat
      421 agatattgta atctgtatga tgttgaatac ttacttagta aagatggtgc caactataaa
      481 gtgctagaat attttatcaa taatg
//
LOCUS       DAJTPG010000356          502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTPG010000356 DAJTPG010000000
VERSION     DAJTPG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>502
                     /locus_tag="OO230_004351"
     CDS             <1..>502
                     /locus_tag="OO230_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5693823.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASG
                     VHNHFGV"
BASE COUNT          137 a          119 c          105 g          141 t
ORIGIN      
        1 aaaaccgaca gaatccattt gaaagacgca gcgcgaatgc tggaagtttc tgtaatgact
       61 attcgtcgcg atctccatca ggaagatgaa cctctgccac tgaccctact gggtggctat
      121 attgtaatgg tgaataaacc cgcgccatcc atgccagtaa tccatgacgt tccaaaaaat
      181 catcgtgatg acttacctat tgcaattctg gctgccggaa tggttaatga aaatgatctg
      241 atcttctttg ataatggcca ggagatacca ctcgttataa gcatgatccc ggatgcaatc
      301 accttcaccg gcatctgtta ctcacatcgc gtctttgttg cgttgaatga aaagcctaat
      361 gtaacagcaa tactttgtgg tggtacgtat cgtgccagaa gtgatgcttt ttacgatgcc
      421 agtaactctt cgccattaga ctctctcaat ccgcgaaaaa tatttatttc cgccagcggt
      481 gtgcataatc actttggcgt ca
//
LOCUS       DAJTPG010000357          492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTPG010000357 DAJTPG010000000
VERSION     DAJTPG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>492
                     /locus_tag="OO230_004352"
     CDS             <1..>492
                     /locus_tag="OO230_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5693824.1"
                     /translation="AWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQGRD
                     NRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYISALG
                     AHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRADSKE
                     PLVV"
BASE COUNT          117 a          135 c          128 g          112 t
ORIGIN      
        1 gcgtgggcgg cagtgagcta catctggcct gactggaagt ttcgcaatct gccgcgaatg
       61 ctcgaacgcg ccacagaggc caactgtcgc tatctcgatg ccatactgga gcaataccat
      121 caggggcgtg ataaccgtct ggcgtatcgt attgcccgcc gcgatgcaca caaccgtgat
      181 gctgaactgg cgtcggtggt atcaaatatg tccagcgaac cgaacgttac cccgcaaatt
      241 cgcgaagccg cgtttcgctt gctgtgcctt aaccatacgt ttaccagcta tatctcagcc
      301 ctcggtgctc accgggagca gttaactaat cctgaaattc tggcgtttct tgatgacgca
      361 gtttgctatg ttgatgacgc gttacatcat caacctgctg atgaagaacg cgtcaatcag
      421 gcattagctg gcctgaaaca gcggatgcag caacttgaac cacgggcaga tagcaaagaa
      481 cctctggtcg ta
//
LOCUS       DAJTPG010000358          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTPG010000358 DAJTPG010000000
VERSION     DAJTPG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>490)
                     /locus_tag="OO230_004353"
     CDS             complement(<1..>490)
                     /locus_tag="OO230_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5693825.1"
                     /translation="SCCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQ
                     FWLESGCVYGYRKIHLDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIV
                     SPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDI
                     VLN"
BASE COUNT          112 a          137 c          126 g          115 t
ORIGIN      
        1 gttcaggaca atgtcctttg tcatccggga ttgcatggac cagccgataa ttttgcgtga
       61 gaacagatca acaacaacgg caagatacag ccagccttcg tgggtcctga tgtaggttat
      121 gtccgttacc caacgctcat caggagcatc cggattgaac tgtcgctgga gcctgttggg
      181 tgacacgata ctggcctcgc ctttacgtgc ccgcgggctt cggtatccga cctgagcctt
      241 tattccgaca cgtttcatca gtctccagac tctgtttact ccgcactgtt gcccgctgtc
      301 acgcagatcc agatggattt tgcgataacc atagacgcat cccgattcca gccagaactg
      361 tttaatctgt cctgtcagtc tcaggtctgc ctgatggcgt tgtgaatgcg gctgctgaag
      421 ccaggcgtaa aaaccactgg gatgaacatc cagcacccga cagagcaggc gaacaggcca
      481 gcaacaggag
//
LOCUS       DAJTPG010000359          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTPG010000359 DAJTPG010000000
VERSION     DAJTPG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..271)
                     /locus_tag="OO230_004354"
     CDS             complement(<1..271)
                     /locus_tag="OO230_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5693826.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(365..>490)
                     /locus_tag="OO230_004355"
     CDS             complement(365..>490)
                     /locus_tag="OO230_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5693827.1"
                     /translation="ILDINDPNFPEQYQRLLVRLDYLTKLDTKASGQRNFGYSGF"
BASE COUNT          155 a           97 c          104 g          134 t
ORIGIN      
        1 tacaccagac atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca
       61 cagcttccct actccagagg taatataaag gggtgggctc gactgattta tctttacgtc
      121 gcttacactg caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca
      181 gagaaggaat tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa
      241 ttttaggatg tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg
      301 cgttacagga aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa
      361 taggtcaaaa accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg
      421 tcaaataatc cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa
      481 tatccaggat
//
LOCUS       DAJTPG010000360          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTPG010000360 DAJTPG010000000
VERSION     DAJTPG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(263..>489)
                     /locus_tag="OO230_004356"
     CDS             complement(263..>489)
                     /locus_tag="OO230_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5693828.1"
                     /translation="QLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSA
                     ASAEQHNNSINHIYPRIARVRSVEEILHAL"
BASE COUNT          122 a          110 c          123 g          134 t
ORIGIN      
        1 atgcatgggg caaaaatatt tgttgtgggg cattttgggg catgattggg acactttgtt
       61 acatatgaac tttgccgatt ttcatatgac atggcaatcc aacgctttga ttattctaaa
      121 gaaaacacat gctcttgggc gttctttagt gatttttaaa aatcccgtat cacgcagtta
      181 aagtggcggg catactcttc aaggctggtg atccccaacc gcacccattt aggatgtgac
      241 cattgcggta gaccgatgta aatcataacg cgtgaaggat ctcttccacg ctacgcacac
      301 gggcgatgcg cgggtagata tgattaatgc tgttattgtg ttgctcggcg ctggcggcac
      361 tacaggcgtc ttcggcaatc accagattaa agccaagttc ccaggcattg cgggcggtgg
      421 attcaacacc gatattggtc gagatcccac ataacactat tgtatcgata cctcggcggc
      481 gcagttgca
//
LOCUS       DAJTPG010000361          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTPG010000361 DAJTPG010000000
VERSION     DAJTPG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..100
                     /locus_tag="OO230_004357"
     CDS             <1..100
                     /locus_tag="OO230_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5693829.1"
                     /translation="YPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(101..484)
                     /locus_tag="OO230_004358"
     CDS             complement(101..484)
                     /locus_tag="OO230_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5693830.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
BASE COUNT          145 a          132 c          101 g          107 t
ORIGIN      
        1 ttacccaaac cgccagtata tgtcctgcgg cttgcgcgca tttctcgatt atgtaaaaac
       61 gggtcagatc tgccagagtc agcgtcaccg accacaataa ttatttgcta ttcgctttac
      121 gcgccagcag cgggaatatc agtcctaatc ctaccagcac aaacggcgtt gcaacattca
      181 gcgccaactg gaaggtccac tctgcagtga aggcttccat tttcgggaag atccccgtca
      241 aacaggcaca ggcggtaaag gcaaaacacc aaatcccgac ggtcattgcc agcggcttat
      301 tacgaataaa gacatattct ggtttatatt tctgcgccag gcgaacgact gcaataaatg
      361 caacaaatac ccataaataa cgcagcggca tcactaccga gttaagattc aacagccatt
      421 tatagagatt gttcatatca ccgatgccga gagtcggcag cataatcaga attgccacca
      481 gcacc
//
LOCUS       DAJTPG010000362          477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTPG010000362 DAJTPG010000000
VERSION     DAJTPG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..322
                     /locus_tag="OO230_004359"
     CDS             <1..322
                     /locus_tag="OO230_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HCR5693831.1"
                     /translation="RLGNMHQQTIAEMIDSPQQQVFGEDKFKQLPAQCRSCNVLKACW
                     GGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLLAHGRPASDIMQAHLM
                     VVNK"
BASE COUNT          115 a          108 c          135 g          119 t
ORIGIN      
        1 tcggctgggg aatatgcatc agcaaacaat tgcagaaatg atcgattccc cgcaacagca
       61 ggtgtttggt gaagataaat ttaagcaatt accggcgcag tgtcgcagtt gtaacgtgtt
      121 aaaagcatgt tggggaggct gcccgaaaca ccgcttcatg ctcgatgcca gcggcaaacc
      181 ggggctgaat tatttgtgtg ccgggtatca gcgttatttc cgccatctac cgccatatct
      241 taaagcaatg gctgatttgc tggcgcacgg tcgcccggcc agtgacatta tgcaggcaca
      301 tttgatggtg gtgaataagt aaaaaagcgg ccatttggaa gccgcctgcg gttgattgcc
      361 ggatgcggcg taaacgcctt atccggccta catgatcgtg caaattcaat aaattgcagc
      421 gttctgtgga tactggtttg ggtgatcgta tccggcaaag cctttacgtg cggtggc
//
LOCUS       DAJTPG010000363          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTPG010000363 DAJTPG010000000
VERSION     DAJTPG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          170 a           83 c           67 g          150 t
ORIGIN      
        1 aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc cggtaaatac
       61 ttataccgga gtattattgc caaaataata acagccccgt caacacatcg ttggcgggga
      121 ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa aataataacc
      181 aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg aggcacagaa
      241 agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct ctattacatt
      301 gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat taggattttg
      361 ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta cttttttaat
      421 gcgatccaat cattttaagg agtttaaaat ggataagaag caggtaacgg
//
LOCUS       DAJTPG010000364          468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTPG010000364 DAJTPG010000000
VERSION     DAJTPG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..380
                     /locus_tag="OO230_004360"
     CDS             <1..380
                     /locus_tag="OO230_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693832.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT          120 a          120 c          114 g          114 t
ORIGIN      
        1 tcggaatgaa ttatcaatta tatgtccgta attacagtcg taacaccaac tacgagatcg
       61 ttgctacggc tgatggcctg gatgtgctga acggcaagca aggttcgctg aataacaacg
      121 gctatattgt gaatgccgga gattcgttag tgattaaagg attccgcaaa gataaacata
      181 ctgaagcggc ttttcagttc gccaacgttg ctgattccta cgcagccaat tcggcgcaag
      241 gcgatgtgcg taataccggg gtaatcggct ttgccgcctt tgaattacaa ggcccggcgc
      301 aaaacgcgct gccgccgtgc tctggacaag cctttccggc agataacaat ggctatgcgc
      361 cgccaccgtg tcggaaatag caacctcgct tgatgcctcg cccatttaag gcgataatta
      421 gcacaaactt gtgtttcgca aatccaaccc ttcctcgttg ggcctaac
//
LOCUS       DAJTPG010000365          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTPG010000365 DAJTPG010000000
VERSION     DAJTPG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..237)
                     /locus_tag="OO230_004361"
     CDS             complement(<1..237)
                     /locus_tag="OO230_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR5693833.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKA"
BASE COUNT          116 a           97 c          105 g          148 t
ORIGIN      
        1 ggcttttgct tcaatcacgg ctaagggaat gcctggcttg tggtacagca cgatgtcagc
       61 agattttacc gttctgcgtg ccgcaacttt accacgtacg atgactttac cgtcccggag
      121 tttgacctcc tgtctgattt gcgttgtgtt gtcccagcct gcattgagaa tggctggcat
      181 aacgcatttg gtaatgatgt ctgcttccgt caggttactt aggttcagtt ccgccatggg
      241 gggttctcgc ttttatcagg acgattctga ttgtacataa aatcaatagc ctggctatga
      301 tttagggcgg tgtttcaacg taaagtacgt agcagagatc acattgatat ctctgttata
      361 tcttgcttga attaatctgg gcatatcaga gacagataaa cagaagttac aggaaccata
      421 tcaactctaa caaaatatat aaaaatctta tagttgcgtt tctttc
//
LOCUS       DAJTPG010000366          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTPG010000366 DAJTPG010000000
VERSION     DAJTPG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            103..>464
                     /locus_tag="OO230_004362"
     CDS             103..>464
                     /locus_tag="OO230_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5693834.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRL"
BASE COUNT          156 a           99 c          115 g           94 t
ORIGIN      
        1 atctccttac ctcacaacac gacaagcctg cattgcggct cttcagtctc cgctgcatac
       61 tgtccaggtg agcgcgggtg atggcataac agaggaaaga aaatgtcact cttccgcaga
      121 ggtgaaatat ggtacgccag tttcacattg ccgaacggta aaagatttaa acagtctctt
      181 ggaacaaagg acaaaaggca ggcgacagaa ctccatgaca agctaaaggc tgaagcatgg
      241 cgggtcagca aacttggtga aatacctgat ataacgttcg aggaagcgtg tgtcaggtgg
      301 cttgaagaga aagcacataa aaaatcactg gacgatgaca aaagccggat cggattctgg
      361 cttcaacatt tcgcaggaat gcaactaaga gacattactg aatcaaaaat ttattcagca
      421 atgcagaaaa tgacgaaccg gcgtcatgag gaaaactgga gact
//
LOCUS       DAJTPG010000367          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTPG010000367 DAJTPG010000000
VERSION     DAJTPG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>463
                     /locus_tag="OO230_004363"
     CDS             <1..>463
                     /locus_tag="OO230_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5693835.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTPG010000368          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTPG010000368 DAJTPG010000000
VERSION     DAJTPG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            108..>457
                     /locus_tag="OO230_004364"
     CDS             108..>457
                     /locus_tag="OO230_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5693836.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGI"
BASE COUNT          141 a           99 c           99 g          118 t
ORIGIN      
        1 taaatgatag gtctaatcat gatttagggt ctaatcaact taatgatcaa tatctatagt
       61 gatatcacca accgtcaaaa cgaaccgaca actccatgag gaactgaatg atgaataata
      121 aagtcagctt cactaacagc aataatccaa ccatctcttt gtctgcagtg atctatttcc
      181 ctccaaaatt tgatgaaacc cgtcagtatc cggcaattgt ggtatctcac ccaggtgggg
      241 gcgttaaaga gcaaacggcc ggaacctatg ccgaaaagct ggcggaaaag ggatttgtca
      301 ctattgctta tgacgcatct tatcaaggtg aaagtggcgg cgagccgcgt cagttagaaa
      361 acccatacat tcgtactgaa gacgtcagtg ccgtgattga ttacctgaca acgctttctt
      421 atgtcgacaa tacccgaatt ggtgcgatgg gaatttg
//
LOCUS       DAJTPG010000369          456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTPG010000369 DAJTPG010000000
VERSION     DAJTPG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..76
                     /gene="relB"
                     /locus_tag="OO230_004365"
     CDS             <1..76
                     /gene="relB"
                     /locus_tag="OO230_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5693837.1"
                     /translation="AELVEIVKERLRNPKPVRVTLDEL"
     gene            76..363
                     /gene="relE"
                     /locus_tag="OO230_004366"
     CDS             76..363
                     /gene="relE"
                     /locus_tag="OO230_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5693838.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          125 a           88 c          129 g          114 t
ORIGIN      
        1 tgctgaactt gtggagatag tgaaagaacg gcttcgtaat cctaagccag tacgtgtgac
       61 gctggatgaa ctctgatggc gtattttctg gattttgacg agcgggcact aaaggaatgg
      121 cgaaagctgg gctcgacggt acgtgaacag ttgaaaaaga agctggttga agtacttgag
      181 tcaccccgga ttgaagcaaa caagctccgt ggtatgcctg attgttacaa gattaagctc
      241 cggtcttcag gctatcgcct tgtataccag gttatagacg agaaagttgt cgttttcgtg
      301 atttctgttg ggaaaagaga acgctcggaa gtatatagcg aggcggtcaa acgcattctc
      361 tgaaccaaag catgacatct ctgtttcgca ccgaaggtga cacttctgct ttgcgttgac
      421 aggagaagca ggctatgaag cagcaaaagg cgatgt
//
LOCUS       DAJTPG010000370          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTPG010000370 DAJTPG010000000
VERSION     DAJTPG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            53..>452
                     /locus_tag="OO230_004367"
     CDS             53..>452
                     /locus_tag="OO230_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5693839.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANR"
BASE COUNT          133 a          107 c          114 g           98 t
ORIGIN      
        1 agtaggtcag tggggtgcga cttcagggtg caaacaaaag gagtacacgt aaatgtccca
       61 acaagaaatc agcatcatta acctcgacca gcttgtgtcc atgacctcag ttgaaattgc
      121 tgagctgaca ggaaaagaac ataaacacgt tttacgagat attcgtaata tggtcgaaga
      181 gcttaacggg gccaaaactg agcactgctc aactttgagc agcgagctta acgggtccaa
      241 atttgggctc gttggcgagg aagtatacaa ggacgcgaaa ggtgagagcc gaacaatgta
      301 tcgtctcgac cgtaagcata ctttcattct ggtcgctggc tattccgttc acctccgcgc
      361 caagtgttac gaccatattc agacgttaga gcgtcgagtc cttcaactgg aagaccagaa
      421 gaagcgagca gccattcagt ccgctaaccg cc
//
LOCUS       DAJTPG010000371          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTPG010000371 DAJTPG010000000
VERSION     DAJTPG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            230..>440
                     /gene="ydeI"
                     /locus_tag="OO230_004368"
     CDS             230..>440
                     /gene="ydeI"
                     /locus_tag="OO230_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5693840.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNL"
BASE COUNT          142 a           83 c           83 g          132 t
ORIGIN      
        1 gtatgctcgt ccaatataat aattttcaaa catataaatc cgattattct gaaaacatgt
       61 ttaagactct tcaatcttct taactttagt gagtcaaatt ctctgaatat ttgacagcta
      121 gtgggctgat tttaaatgcc ttataacaat tggcagtatt cttatttggc aattataaaa
      181 atagcaacca gacttaataa gtcaaaccaa acgcaattaa ggagttagta tgaaatttca
      241 gtccattgta ttagcaagtt ttcttgtcat gccttatgcg ttggcagatg accagggcgg
      301 tttaaaacag gatgcagcgc caccgccgcc tcatgcaata gaagatggct atcgcggaac
      361 cgatgatgca aaaaaaatga ccgttgattt cgcaaaaaac atgcacgatg gcgcttcggt
      421 ttcactacgc ggtaatttga
//
LOCUS       DAJTPG010000372          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTPG010000372 DAJTPG010000000
VERSION     DAJTPG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            35..434
                     /locus_tag="OO230_004369"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO230_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTPG010000373          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTPG010000373 DAJTPG010000000
VERSION     DAJTPG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>429
                     /locus_tag="OO230_004370"
     CDS             <1..>429
                     /locus_tag="OO230_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693841.1"
                     /translation="TGQVWRTARILKDRSTRIDGQVFSNPTFKNFLPHIESLNLGGEH
                     PGIDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGTSNDAKRAKADLL
                     EKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDP"
BASE COUNT          137 a          105 c           97 g           90 t
ORIGIN      
        1 accggtcagg tgtggcgcac cgccagaata ctaaaagatc gctccacccg tattgatgga
       61 caggtatttt caaacccaac atttaaaaac tttcttcctc atatcgagtc tcttaacctg
      121 ggaggtgaac accccggcat cgatacctca cgggataaca gtgaattatt tgccaacgac
      181 ggtacaccga catggctaaa agaaaagcgt gcaaaagaaa tcgcacttga tgaaattatc
      241 gagttgctca acaagcacca tgggggcacc agtaacgacg ccaaacgcgc aaaagctgat
      301 ttgctggaaa aaacattcac ctcccgctca tgggaaagaa tcaaaggtat ggactggccg
      361 acaatcaaag ctggtcgtaa tgcactatgg attgaactgg aaggcgttga atatgcattc
      421 ccggatcca
//
LOCUS       DAJTPG010000374          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTPG010000374 DAJTPG010000000
VERSION     DAJTPG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>429
                     /gene="ipaH3"
                     /locus_tag="OO230_004371"
     CDS             <1..>429
                     /gene="ipaH3"
                     /locus_tag="OO230_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5693842.1"
                     /translation="SELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASLEYL
                     DACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLE
                     VLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHH"
BASE COUNT          129 a          115 c           69 g          116 t
ORIGIN      
        1 agtgagcttc aactgaatcg tttaaatctg tcctcgctac ctgacaactt accacctcaa
       61 atcactgttc tggaaattac tcagaatgcc ctaatatcat taccagaatt gccagcatcg
      121 ctggaatacc ttgacgcctg tgacaatcgc ctgtcaacac ttcctgaatt acccgcatct
      181 ctgaaacatc ttgatgtaga taacaaccaa ctaaccatgc ttcctgaatt gcctgcattg
      241 ctggaatata ttaatgcaga taacaatcag ctaaccatgc ttcctgaatt acctacatcg
      301 ctggaagtgc tctcagtaag aaataaccag ctgacatttc tccctgagtt acctgaatca
      361 ctggaagcgc tcgatgtaag tactaatctt ctggaaagcc taccagccgt acctgtaaga
      421 aatcatcac
//
LOCUS       DAJTPG010000375          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTPG010000375 DAJTPG010000000
VERSION     DAJTPG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..423
                     /locus_tag="OO230_004372"
                     /pseudo
     CDS             1..423
                     /locus_tag="OO230_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          111 a          111 c          116 g           85 t
ORIGIN      
        1 attgaaatgg aaaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga
       61 acagttttcg ataatcggga aactcagggc gcgttatcct gtggccactc tctgccatgt
      121 gttcggggtc catcgcagca gctacaaata ctggaaaaac cgtcctgaaa aaccagacgg
      181 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
      241 cggagcaaga agcatcgcca caatggcaac ccagagaggc taccagatgg ggcgctggct
      301 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
      361 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
      421 aac
//
LOCUS       DAJTPG010000376          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTPG010000376 DAJTPG010000000
VERSION     DAJTPG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>421)
                     /locus_tag="OO230_004373"
     CDS             complement(<1..>421)
                     /locus_tag="OO230_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5693843.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNT"
BASE COUNT           99 a          122 c          103 g           97 t
ORIGIN      
        1 gtattgttgg agagcttttt catcatcccc gccataaagc cgacttcacg accaccaacg
       61 ccgatatcac ctgccggaac gtcggtatcc gcgcccaggt ggcgatacag ttcagtcatc
      121 agcgtctggc aaaaacgcat cactttacct tcgctttttc ctttcggatc gaaatcgctg
      181 ccgcctttac caccgcccat cggcagggta gtcagggcat ttttgaaggt ttgttcaaag
      241 ccgaggaatt tgagaatgga aaggttaact gacggatgga agcgcatacc gcctttgtac
      301 gggccgatgg cagagctgaa ctgcacacgc catgcacggt tgacctgtat ctggttgcga
      361 tcatcaaccc ataccacgcg aaactggatc acgcgctccg gttcaaccag acgctccagt
      421 a
//
LOCUS       DAJTPG010000377          419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTPG010000377 DAJTPG010000000
VERSION     DAJTPG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(226..>419)
                     /locus_tag="OO230_004374"
     CDS             complement(226..>419)
                     /locus_tag="OO230_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693844.1"
                     /translation="DMAMFLSPLPVVLEWHCVMCEQFFHGKKFCEACGTGIYCRAVCT
                     KSVYSYQPDLFEDCGGAST"
BASE COUNT          116 a          121 c           93 g           89 t
ORIGIN      
        1 tcgcgtatgg ttgattgaca ccacagtgcg aattcggcga atgcgagatc acggtacgat
       61 tccgcaccac actaccaatc aataccccgg gttttattca ggtaagcagg actgcggata
      121 tccggtgtcg cgcctttgtg tcacgaacgg ggtgggtgcg aaacaccaga taaaatacag
      181 gttggcaata cctctgaacg ccctgagcag gtcgggcatt tcggattaag tactcgcacc
      241 tccgcagtcc tcaaacaagt ctggctggta gctgtaaaca gacttcgtac ataccgctct
      301 gcaataaatc cccgtaccac aggcctcaca gaacttcttc ccatgaaaaa actgctcaca
      361 catcacacaa tgccactcca gcacgaccgg taatggcgat aaaaacatcg ccatatcct
//
LOCUS       DAJTPG010000378          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTPG010000378 DAJTPG010000000
VERSION     DAJTPG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          127 a           81 c           67 g          138 t
ORIGIN      
        1 cctcgtatca gggggctggt ttaggtgttc aacatcaacg tcttccatga taattaacat
       61 ggaagacgtt tccatcgtat tattcatttc atcactgttt tagcatcacg tttcaatagt
      121 atcctttcat agggctaccc aggatgcggt caccttcatt gtcgctctaa gcgactagat
      181 catcagaaat ctccagttag attgcatact tccttacaca ctttcgtgaa acttcagggt
      241 aaggtgagaa agccgtcatc cctttttcta attccagcat ctaaataaat tccgaatttt
      301 gttattagta tcgctaccgc aataaaaaca gatagtaaag agagtaatat atactttatt
      361 gatatattaa gaataattaa ttgttaacat atctaatcca ataagatgtt atg
//
LOCUS       DAJTPG010000379          399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTPG010000379 DAJTPG010000000
VERSION     DAJTPG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..254)
                     /locus_tag="OO230_004375"
     CDS             complement(<1..254)
                     /locus_tag="OO230_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693845.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..>399
                     /gene="relB"
                     /locus_tag="OO230_004376"
     CDS             279..>399
                     /gene="relB"
                     /locus_tag="OO230_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5693846.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIAD"
BASE COUNT          114 a           82 c           93 g          110 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgaca
//
LOCUS       DAJTPG010000380          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTPG010000380 DAJTPG010000000
VERSION     DAJTPG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..342
                     /gene="metG"
                     /locus_tag="OO230_004377"
     CDS             <1..342
                     /gene="metG"
                     /locus_tag="OO230_004377"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HCR5693847.1"
                     /translation="DPIQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGE
                     KRNVFSGIRSAYPDPQALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSP
                     DAGAKPGHQVK"
BASE COUNT           89 a          105 c          102 g          100 t
ORIGIN      
        1 gatccgattc aggaaaccat cacctttgac gacttcgcca aagttgacct gcgcgtggcg
       61 ctgatcgaaa acgcagagtt tgttgaaggt tctgacaaac tgctgcgcct gacgctggat
      121 ctcggcggtg aaaaacgcaa tgtcttctcc ggtattcgtt ctgcttaccc ggatccgcag
      181 gcactgattg gtcgtcacac cattatggtg gctaacctgg caccacgtaa aatgcgcttc
      241 ggtatctctg aaggcatggt gatggctgcc ggtcctggcg ggaaagatat tttcctgcta
      301 agcccggatg ccggtgctaa accgggtcat caggtgaaat aatccccctt caaggcgctg
      361 catcgacagc gccttttctt tataaattcc taaagt
//
LOCUS       DAJTPG010000381          392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTPG010000381 DAJTPG010000000
VERSION     DAJTPG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>392
                     /locus_tag="OO230_004378"
     CDS             <1..>392
                     /locus_tag="OO230_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5693848.1"
                     /translation="YRANVARLEAKIKSSKANLEGIQATITMQQSIIQSASETWQAVK
                     HEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQQVAAKERKAANDFLVEKQRLAVL
                     SAQEENVRASIEEVQAALTQALLDLEYT"
BASE COUNT          131 a           87 c           98 g           76 t
ORIGIN      
        1 ttatcgtgca aatgtcgcca ggcttgaggc aaaaataaaa agtagcaaag ccaatctcga
       61 aggcattcaa gccactatta cgatgcagca gtcgataatt caaagtgcca gtgaaacatg
      121 gcaggcagta aaacatgagg agcaaaagcg cctgcgagat actgaacgtt atgaaaaact
      181 cgcacaatca gcggcaatat cgcaacaaat tattgataac gccaggtttg actaccagca
      241 ggtcgcagca aaggagcgca aagccgcgaa cgatttcctg gtggagaagc aacggctggc
      301 agttttatct gctcaggaag aaaacgtacg tgcgagtatc gaagaagttc aggccgcttt
      361 gactcaggca ctactggatc tggaatatac cc
//
LOCUS       DAJTPG010000382          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTPG010000382 DAJTPG010000000
VERSION     DAJTPG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(63..>388)
                     /locus_tag="OO230_004379"
     CDS             complement(63..>388)
                     /locus_tag="OO230_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5693849.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
BASE COUNT          112 a           82 c           83 g          111 t
ORIGIN      
        1 agctggagtt agagcactga tataacgggc ctgatggccc gttttagtgt ttgtattaga
       61 tgctagttat aggtgatttt gaggattacg tctttactaa acggtccctc aacaacagtc
      121 tctgccgggt tcgcactaag ttctgccctg aatggtgcgg tgaccgaagg tgcactagac
      181 gatgaaaaag aagcaaattg tgagaagcgg ttaaattccg ttgctgttcc atctttatga
      241 aatatcttca atagcagtcc attacccata tccagatggc tataatcact caaaatatcc
      301 gtagtgaaaa agctccctaa aatattgaat tgctcagtac aggccgcacc aggatctttt
      361 gtcatgacta cactaaacgt ctcactaa
//
LOCUS       DAJTPG010000383          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTPG010000383 DAJTPG010000000
VERSION     DAJTPG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            1..387
                     /locus_tag="OO230_004380"
                     /pseudo
     CDS             1..387
                     /locus_tag="OO230_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT           88 a          115 c          101 g           83 t
ORIGIN      
        1 atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag acactcacca
       61 cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact ctccaggcgc
      121 tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg gcttcggcac
      181 gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat accacccgtc
      241 tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc ctcgctcgcc
      301 cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct ggtgtggtgc
      361 ggtgaaaatc gaacctccgc ccaacag
//
LOCUS       DAJTPG010000384          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTPG010000384 DAJTPG010000000
VERSION     DAJTPG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          133 a           64 c           71 g          116 t
ORIGIN      
        1 caattcaatc aaatagagat tggctaagca tgtctatctt ttctgattac agttccagta
       61 gtgaaatgca caataattta acaattgatt attatttagc actttcctct accaaaggca
      121 gtggaattac taatatcatt tcaattattc ttcagcaagc ccaggattat gatgtggcga
      181 aaataacatg atggtgagga gaagcagtta aggaaatcct tataactaac tgtaatttga
      241 gaagaatatt tttacctatt ccttttgctt ttgataactg cgcgggaaca aagtaaaaat
      301 aatgacgaag taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag
      361 gccggggaaa atccccttaa tacc
//
LOCUS       DAJTPG010000385          380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTPG010000385 DAJTPG010000000
VERSION     DAJTPG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(272..>380)
                     /locus_tag="OO230_004381"
     CDS             complement(272..>380)
                     /locus_tag="OO230_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5693850.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          110 t
ORIGIN      
        1 cccgtaaaga caggagtcaa gttcccgctc cgacataact gcgtgcaatg gattttgaag
       61 gaaccgtcag cccattcccc ggaggcaaac atgggctggc aaaagtgtag cggtattagg
      121 cacagctatt taggaaagag ggaccatctc catctcatta aatggaccac gccagacaat
      181 tcactgtcat gctgatgctg cactgtgggg acagaatcac tcatgatgac ttcgataatc
      241 gacgacatta ttatttggcc gagcttttag gtcatatctc aaatcgtttg cctaatattg
      301 ctccattttt taataaaaaa tcaatcattt ttgaatcttt gcttttcatt gcattgtcaa
      361 gcatagtgtc cccactattt
//
LOCUS       DAJTPG010000386          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTPG010000386 DAJTPG010000000
VERSION     DAJTPG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.392"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..326)
                     /locus_tag="OO230_004382"
     CDS             complement(<1..326)
                     /locus_tag="OO230_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HCR5693851.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLL"
BASE COUNT           95 a          110 c           87 g           86 t
ORIGIN      
        1 tcgagcaggt cagtcagtgg cagcaaatca tgaatgcaag gctcaccacc cgtaatcacc
       61 acatgccgcg cggtatatcc ctgacgacca atgacagcca gcaagtcttc actgctcgcc
      121 gccccccact tatcactctc tttggtcttc gccagaatgc tgaaaaggga gacttcccga
      181 tcctcaagct tttcccaggt gtgtttggtg tcgcaccagg cacagccaac cgggcatccc
      241 tgtaaacgaa taaaaatggc gggaacgccg gtaaagtaac cctcaccttg cagggtctgg
      301 aacatctcgt taatcgggta ctgcatagca ttctctgtga agtggataat tgttaattat
      361 tgcagatcct gccgcaac
//
LOCUS       DAJTPG010000387          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTPG010000387 DAJTPG010000000
VERSION     DAJTPG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>365
                     /locus_tag="OO230_004383"
     CDS             <1..>365
                     /locus_tag="OO230_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5693852.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAJTPG010000388          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTPG010000388 DAJTPG010000000
VERSION     DAJTPG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.394"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT           76 a          102 c           90 g           91 t
ORIGIN      
        1 agcctgcgct gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg
       61 ccccggaggc gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      121 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg
      181 catctggcca tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc
      241 gaatatcatt catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc
      301 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaa
//
LOCUS       DAJTPG010000389          358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTPG010000389 DAJTPG010000000
VERSION     DAJTPG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..346
                     /locus_tag="OO230_004384"
     CDS             <1..346
                     /locus_tag="OO230_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693853.1"
                     /translation="NGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGNYADAVRL
                     LTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDIISPGAFV
                     KIGPIIRATLNR"
BASE COUNT           82 a           83 c          105 g           88 t
ORIGIN      
        1 taatggtctg gacccggaag tgctggcgga gattaaccgc gaacgcgagg ccttcctggc
       61 ggcacagcag ggaagtactt caacggagtt gtttaccacc attgaaggca actacgctga
      121 tgctgtgcgc ctgctgacga ctgctcatag cgtaccgttt gatggtaaag cgacgctgtt
      181 tgttgctgaa cgcacgcttc aggaaggtat gagccccgaa cgcgcctggt cgccgtggat
      241 tgcggagctg gatatctatc gtcaggattg tgcgcatgtg gatattatct ctccaggggc
      301 gtttgtaaaa attgggccga ttattcgcgc aacgctaaac aggtaaatta atattatc
//
LOCUS       DAJTPG010000390          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTPG010000390 DAJTPG010000000
VERSION     DAJTPG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.396"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          102 a           69 c           70 g          115 t
ORIGIN      
        1 ggaagtaatc cattacgact ataactgaca ggtcatcacg acgtatgact gcgtatttgc
       61 tactaaatag tttctttatt ttttccacga tgcccccagg tttataagta caaacggtta
      121 tatccacata gagacaaaaa tattaatctg ataaatattt atttcacgcc gtatatttga
      181 ttgtttaatg tgcaagtaca atgactttta ttttttgttg tgtatataat caaatatatg
      241 gttatttttc accctgcgta ttcagcacgc aacaaaaaac ccgccgaagc gggtttagtg
      301 cgggtgcgtt gaggatgcct gacacatgag aggtggcgag ggatttctcc cccgcc
//
LOCUS       DAJTPG010000391          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTPG010000391 DAJTPG010000000
VERSION     DAJTPG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.397"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..316
                     /locus_tag="OO230_004385"
     CDS             <1..316
                     /locus_tag="OO230_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5693854.1"
                     /translation="PSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLLLDNLS
                     DYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGEMKNGE
                     AK"
BASE COUNT          109 a           83 c           87 g           74 t
ORIGIN      
        1 gccttcattc caggacgtac tggaatttgt tcgtctgttc cgtcgtaaga acaaactgca
       61 acgtgaaatt caggacgttg agaaaaagat ccgtgacaac cagaagcgcg tcctgttgct
      121 ggacaacctg agcgattaca tcaagccagg gatgagcgtt gaagcaatcc agggcatcat
      181 cgccagcatg aaaggtgact atgaagatcg cgttgacgat tacatcatca aaaatgccga
      241 gctctccaaa gaacgccgcg atatctccaa aaagctgaaa gctatgggcg aaatgaaaaa
      301 cggcgaagcg aagtaattcc cgttttattc aatgagggtt gcccggcaac cct
//
LOCUS       DAJTPG010000392          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTPG010000392 DAJTPG010000000
VERSION     DAJTPG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.398"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          111 a           82 c           81 g           73 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgag
//
LOCUS       DAJTPG010000393          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTPG010000393 DAJTPG010000000
VERSION     DAJTPG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.399"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..149
                     /locus_tag="OO230_004386"
     CDS             <1..149
                     /locus_tag="OO230_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="in yeast cells this enzyme removes the 2'-phosphate
                     from RNA via an intermediate in which the phosphate is
                     ADP-ribosylated by NAD followed by a presumed
                     transesterification to release the RNA and generate
                     ADP-ribose 1''-2''-cyclic phosphate; functions in tRNA
                     splicing; the function and substrate of bacterial enzymes
                     in organisms that do not perform splicing is unknown;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA--NAD 2'-phosphotransferase"
                     /protein_id="HCR5693855.1"
                     /translation="PVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEWSFMPTG
                     VQKQ"
BASE COUNT           89 a           68 c           92 g           85 t
ORIGIN      
        1 cgccggttgt cttaaccgtc aaagcacagg aaatggcgaa acgaggcatc cctttctggc
       61 aagcggaaaa cggggtctgg ctgacatcaa cggttgctgt tgagtttctt gagtggtcgt
      121 ttatgccgac tggggtacaa aaacaataaa ttcaatatat tgcaggggtt atataggcct
      181 ggtaagcgca gcacatccgg caatgtagcg tttgttatca gtctgaaggg ataccctcgt
      241 aaaagggtat cctttaataa caactgctga aattaaagtg atttacggca ggcggtcagt
      301 tggggactgg aatcagcgtc gcttccggtc atac
//
LOCUS       DAJTPG010000394          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTPG010000394 DAJTPG010000000
VERSION     DAJTPG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.400"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            51..>332
                     /locus_tag="OO230_004387"
     CDS             51..>332
                     /locus_tag="OO230_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5693856.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAJTPG010000395          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTPG010000395 DAJTPG010000000
VERSION     DAJTPG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.401"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>331
                     /locus_tag="OO230_004388"
     CDS             <1..>331
                     /locus_tag="OO230_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5693857.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRT"
BASE COUNT           90 a           68 c           84 g           89 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaatcgtac c
//
LOCUS       DAJTPG010000396          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTPG010000396 DAJTPG010000000
VERSION     DAJTPG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.402"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            28..>327
                     /locus_tag="OO230_004389"
     CDS             28..>327
                     /locus_tag="OO230_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693858.1"
                     /translation="MTVAQCLDYWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPA
                     SSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDF"
BASE COUNT           94 a           78 c           79 g           76 t
ORIGIN      
        1 tattttgaag agaaggtgga gaactccatg acggtcgccc agtgtctcga ctactggttc
       61 gacaactacg tctctacaac tctcagagaa aagacccagg cactttatcg atcaacggtt
      121 atgaagcgca tgcatgacgc cttccctaat cgtccggcat cttctatcac ggttaagcaa
      181 tgggttgacc tgcttaccga agaagaaaaa gataatccac gccgagcaag gcaggtgcta
      241 agtcaactaa gatcagcaat tagttggtgc atgcggcgtc agttgataga tagttgcgca
      301 attatgagca tccagccaag ggacttc
//
LOCUS       DAJTPG010000397          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTPG010000397 DAJTPG010000000
VERSION     DAJTPG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.403"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(43..264)
                     /locus_tag="OO230_004390"
     CDS             complement(43..264)
                     /locus_tag="OO230_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693859.1"
                     /translation="MPRVFMEAMPPEWQEKMAQLLFEYSDTIKTDVCGVHSCFVTAKD
                     GNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           89 a           81 c           66 g           85 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt c
//
LOCUS       DAJTPG010000398          320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTPG010000398 DAJTPG010000000
VERSION     DAJTPG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.404"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>320
                     /locus_tag="OO230_004391"
     CDS             <1..>320
                     /locus_tag="OO230_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693860.1"
                     /translation="FMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQ
                     ETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARAN
                     LALA"
BASE COUNT           96 a           79 c           90 g           55 t
ORIGIN      
        1 gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac agaatgcacg
       61 tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa ttctggctga
      121 acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa gaaaggttga
      181 ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg aaatcgccaa
      241 ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg cagaagcgcg
      301 tgctaacctt gcactcgcag
//
LOCUS       DAJTPG010000399          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTPG010000399 DAJTPG010000000
VERSION     DAJTPG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.405"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..175)
                     /gene="osmC"
                     /locus_tag="OO230_004392"
     CDS             complement(<1..175)
                     /gene="osmC"
                     /locus_tag="OO230_004392"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5693861.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAA"
BASE COUNT           81 a           75 c           69 g           94 t
ORIGIN      
        1 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
       61 gcgtgttaaa tccatacggc tgttggttca gcacgccgct ctcggtggat actgttccct
      121 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
      181 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
      241 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
      301 aatagataac cgggaatat
//
LOCUS       DAJTPG010000400          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTPG010000400 DAJTPG010000000
VERSION     DAJTPG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.406"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>316
                     /locus_tag="OO230_004393"
     CDS             <1..>316
                     /locus_tag="OO230_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5693862.1"
                     /translation="LSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTS
                     LGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSA
                     GG"
BASE COUNT           58 a           82 c           86 g           90 t
ORIGIN      
        1 tgctttccgg tatgtatccg tcttctgtaa tggcggcttc cggtgctccg tttgcaatca
       61 gtgcttcaac tatcctcggt aactgggctg cgccgctggt ttctgcattc accgcctttg
      121 cgtgcctgac ttctctgggc tcctggatga tgttggtagg ccaggcaggt gtacgtgccg
      181 ctaacgacgg taacttcccg aaagtttatg gtgaagtcga cagcaacggt attccgaaaa
      241 aaggtctgct gctggctgca gtgaaaatga ctgccctgat gatcctcatc actctgatga
      301 actctgccgg tggtaa
//
LOCUS       DAJTPG010000401          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTPG010000401 DAJTPG010000000
VERSION     DAJTPG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.407"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
BASE COUNT          121 a           49 c           48 g           97 t
ORIGIN      
        1 gacaccaagc gttctactta aagtgaatgc ttcctgtgtg ctctgggtca gtcgataagt
       61 gaatagcaat aagacacaat catcttaatt atggtctcta gtccctacag gggcttaact
      121 taaagttaac ttaaagacac aattaaagat taacttatag tattacttaa agttaactta
      181 aagacacaat tagagattaa ctttaagtat taaataaaag atttaattaa agaccaatta
      241 aagattaact ctaagggaat accttaaggg gtgatgatat ttataataat caatcaccaa
      301 acctataggt cttct
//
LOCUS       DAJTPG010000402          314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTPG010000402 DAJTPG010000000
VERSION     DAJTPG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.408"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>314
                     /locus_tag="OO230_004394"
     CDS             <1..>314
                     /locus_tag="OO230_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5693863.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IP"
BASE COUNT           89 a           62 c           79 g           84 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccga
//
LOCUS       DAJTPG010000403          313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTPG010000403 DAJTPG010000000
VERSION     DAJTPG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.409"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            153..>313
                     /locus_tag="OO230_004395"
     CDS             153..>313
                     /locus_tag="OO230_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5693864.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCAR"
BASE COUNT           90 a           90 c           71 g           62 t
ORIGIN      
        1 gctgcatccg actcttttat caacgcttac gtaattaagg gacacagcct aaatagctgc
       61 gcggaatagt agatcccttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc
      121 aaatcaaaac aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg
      181 acgaacgacg cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca
      241 gactgactgc catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc
      301 tctgctgcgc ccg
//
LOCUS       DAJTPG010000404          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTPG010000404 DAJTPG010000000
VERSION     DAJTPG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.410"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..254
                     /locus_tag="OO230_004396"
     CDS             <1..254
                     /locus_tag="OO230_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5693865.1"
                     /translation="LFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMA
                     MCFSLVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT           85 a           74 c           80 g           72 t
ORIGIN      
        1 gcctgtttgc gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg
       61 attacattcg tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg
      121 atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc
      181 ttgatcccaa atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta
      241 gccaggcagc ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt
      301 cggcacattg t
//
LOCUS       DAJTPG010000405          307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTPG010000405 DAJTPG010000000
VERSION     DAJTPG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.411"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(196..270)
                     /gene="tnaC"
                     /locus_tag="OO230_004397"
     CDS             complement(196..270)
                     /gene="tnaC"
                     /locus_tag="OO230_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5693866.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
BASE COUNT          109 a           57 c           53 g           88 t
ORIGIN      
        1 tacagtgatc cctgtgaata ttacatctgc aatatccatt aattcaccat gtatatatac
       61 aagcaccatc atttactaaa ataagtaaat aaacgagaga tggcctttgc aaaaggcaaa
      121 attaagatat agcaaactaa tcgatcacat tgaatctaat cggtttggct ctggtgatgg
      181 ctacagaagg gtaaattaag ggcggtgatc gacaatctta ttgtcgatat tgaaccattt
      241 tgaggtcaca catacatgta agatattcat aatgcactta tcctcgaaag acacgccgct
      301 acattat
//
LOCUS       DAJTPG010000406          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTPG010000406 DAJTPG010000000
VERSION     DAJTPG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.413"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(57..251)
                     /locus_tag="OO230_004398"
     CDS             complement(57..251)
                     /locus_tag="OO230_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5693867.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT           77 a           81 c           56 g           86 t
ORIGIN      
        1 tcatcgtagc ctttaaaaac gaattttaaa ggcaccctaa aggcaggtca atgaagttaa
       61 cccgccttcg cgtttacact ctctgagaga atactagctt caatctcttc caaaatctct
      121 ctgacttgct cgtcgggcat atcaacgagc atcaacgctg ccaggcgttt acggccctct
      181 agttgcagta gctcttgagt ttccttatct aacaagcgta gcacagtctc aacacctttc
      241 cttgcgagca tgtttgtaag ggcttctcgt tcctcactgg taagaaacga caacatttct
//
LOCUS       DAJTPG010000407          295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTPG010000407 DAJTPG010000000
VERSION     DAJTPG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.414"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..>295)
                     /locus_tag="OO230_004399"
     CDS             complement(<1..>295)
                     /locus_tag="OO230_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5693868.1"
                     /translation="YWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLSLSGRLAIKII
                     AEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGLPVPGGRLGIKI"
BASE COUNT           70 a           87 c           66 g           72 t
ORIGIN      
        1 gatttttatc cccaaccgac caccaggaac aggcagtccg cgcacaatat tggtttcatc
       61 aaactgctcg tcgtcgataa gcaaccccgc atgtgtcagt gcatccagtg gtgctttcag
      121 gatattgtcc aggtcacgac gccgcttatc cggtggctct gcaataatct ttatcgccag
      181 tcttccggac aggcttaatt tcagtcgctg ctgacggaca ataagcgcca catcccggcg
      241 ataacgctca ccggcttttg atacaaaata tgtgctgcca cgacgtcgcc agtaa
//
LOCUS       DAJTPG010000408          293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTPG010000408 DAJTPG010000000
VERSION     DAJTPG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.415"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..124)
                     /locus_tag="OO230_004400"
     CDS             complement(<1..124)
                     /locus_tag="OO230_004400"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5693869.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLT"
BASE COUNT           97 a           58 c           72 g           66 t
ORIGIN      
        1 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
       61 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
      121 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
      181 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
      241 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taa
//
LOCUS       DAJTPG010000409          289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTPG010000409 DAJTPG010000000
VERSION     DAJTPG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.denovo.417"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..>289
                     /locus_tag="OO230_004401"
     CDS             <1..>289
                     /locus_tag="OO230_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5693870.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DAJTPG010000410         5818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.guided.168, whole genome shotgun sequence.
ACCESSION   DAJTPG010000410 DAJTPG010000000
VERSION     DAJTPG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.guided.168"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO230_004402"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO230_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5693871.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2123
                     /locus_tag="OO230_004403"
                     /pseudo
     CDS             1596..2123
                     /locus_tag="OO230_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2144..2617
                     /locus_tag="OO230_004404"
     CDS             2144..2617
                     /locus_tag="OO230_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5693872.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2692..2967
                     /locus_tag="OO230_004405"
                     /pseudo
     CDS             2692..2967
                     /locus_tag="OO230_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2967..3803
                     /locus_tag="OO230_004406"
     CDS             2967..3803
                     /locus_tag="OO230_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5693873.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3910..4314)
                     /locus_tag="OO230_004407"
                     /pseudo
     CDS             complement(3910..4314)
                     /locus_tag="OO230_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4358..4747)
                     /locus_tag="OO230_004408"
     CDS             complement(4358..4747)
                     /locus_tag="OO230_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5693874.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4965..5405
                     /locus_tag="OO230_004409"
     CDS             4965..5405
                     /locus_tag="OO230_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5693875.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1398 a         1539 c         1518 g         1363 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgttaacc caggataaga accttgaaag tatcattgat
     2161 agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct ggtccgcgaa
     2221 aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg tgggtcgcaa
     2281 gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta cccgctcagg
     2341 ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg ccatggacag
     2401 gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt accgagaaac
     2461 attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag agggggatgt
     2521 tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact ttacttcaaa
     2581 cattaactat tgctatcaaa tttggaaaaa gggttaacaa agctatgcaa tcgacggcaa
     2641 aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga ttgatagcga cgatatggtt
     2701 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
     2761 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
     2821 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
     2881 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
     2941 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
     3001 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
     3061 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
     3121 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
     3181 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
     3241 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
     3301 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     3361 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     3421 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     3481 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     3541 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     3601 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     3661 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     3721 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     3781 aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc
     3841 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     3901 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3961 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4021 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4081 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4141 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4201 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4261 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4321 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4381 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     4441 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     4501 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     4561 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     4621 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     4681 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     4741 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     4801 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     4861 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     4921 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     4981 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     5041 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     5101 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     5161 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     5221 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     5281 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     5341 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     5401 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     5461 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     5521 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     5581 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     5641 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     5701 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     5761 acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa gtgatgta
//
LOCUS       DAJTPG010000411         5092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.guided.184, whole genome shotgun sequence.
ACCESSION   DAJTPG010000411 DAJTPG010000000
VERSION     DAJTPG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.guided.184"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            264..923
                     /locus_tag="OO230_004410"
     CDS             264..923
                     /locus_tag="OO230_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5693876.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="OO230_004411"
     CDS             complement(1124..1501)
                     /locus_tag="OO230_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5693877.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="OO230_004412"
     CDS             complement(1568..4534)
                     /locus_tag="OO230_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5693878.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5073)
                     /locus_tag="OO230_004413"
     CDS             complement(4537..5073)
                     /locus_tag="OO230_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5693879.1"
                     /translation="MRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQLEALISFA
                     RTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSPMANLMLSV
                     MGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAGEQKTKLA
                     REFGISRETLYQYLRTDQ"
BASE COUNT         1023 a         1393 c         1528 g         1148 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc ca
//
LOCUS       DAJTPG010000412         3801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.guided.209, whole genome shotgun sequence.
ACCESSION   DAJTPG010000412 DAJTPG010000000
VERSION     DAJTPG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.guided.209"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(171..521)
                     /locus_tag="OO230_004414"
     CDS             complement(171..521)
                     /locus_tag="OO230_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5693880.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(724..1737)
                     /gene="intI1"
                     /locus_tag="OO230_004415"
     CDS             complement(724..1737)
                     /gene="intI1"
                     /locus_tag="OO230_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5693881.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1947..2777
                     /locus_tag="OO230_004416"
     CDS             1947..2777
                     /locus_tag="OO230_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5693882.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2890..3681
                     /gene="aadA1"
                     /locus_tag="OO230_004417"
     CDS             2890..3681
                     /gene="aadA1"
                     /locus_tag="OO230_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5693883.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1002 a          986 c          979 g          834 t
ORIGIN      
        1 gtcgaaggtg ctgaccctga tatacccaat gcgctgtcca gtcatggaat tccctgcaaa
       61 atgtcaggga agactctatg accttcaacg agatatgtca ataaattcaa aattcaatcc
      121 tatcctgacg caatttacac atggcatctg acatcaggtt agggtatgcc tcaacctgac
      181 ggcggcgaat cacaagcgtc cggtttgacg ctggtttcgg tcgcagtgct ggcccgcgcc
      241 tggaagcgct gatagcactc cagcccgcag aaatgctcca cgtactcggc cccttccggc
      301 gtgaaggcgg catcgagcgg gatttccttg cagcacacgc agcaactggt gctcggttca
      361 ttggcgttca tggtggtgtt cctccattgg ttgacgaagc cgacgaaggc cgccgccggc
      421 atcggcctgg cgaacaggaa accctgcacc gtgtcgcaac ccgcctgccg caaccacgca
      481 aggcagtcgg gtgtttccac accctcggct accacctcca ttgtcgtttt cagaagacgg
      541 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tccatcaggc
      601 aacgacgggc tgctgccggc catcagcgga cgcagggagg actttccgca accggccgtt
      661 cgatgcggca ccgatggcct tcgcgcaggg gtagtgaatc cgccaggatt gacttgcgct
      721 gccctacctc tcactagtga ggggcggcag cgcatcaagc ggtgagcgca ctccggcacc
      781 gccaactttc agcacatgcg tgtaaatcat cgtcgtagag acgtcggaat ggccgagcag
      841 atcctgcacg gttcgaatgt cgtaaccgct gcggagcaag gccgtcgcga acgagtggcg
      901 gagggtgtgc ggtgtggcgg gcttcgtgat gcctgcttgt tctacggcac gtttgaaggc
      961 gcgctgaaag gtctggtcat acatgtgatg gcgacgcacg acaccgctcc gtggatcggt
     1021 cgaatgcgtg tgctgcgcaa aaacccagaa ccacggccag gaatgcccgg cgcgcggata
     1081 cttccgctca agggcgtcgg gaagcgcaac gccgctgcgg ccctcggcct ggtccttcag
     1141 ccaccatgcc cgtgcacgcg acagctgctc gcgcaggctg ggtgccaagc tctcgggtaa
     1201 catcaaggcc cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa
     1261 atccagatcc ttgacccgca gttgcaaacc ctcactgatc cgcatgcccg ttccatacag
     1321 aagctgggcg aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc
     1381 cggggtcagc accaccggca agcgccgcga cggccgaggt cttccgatct cctgaagcca
     1441 gggcagatcc gtgcacagca ccttgccgta gaagaacagc aaggccgcca atgcctgacg
     1501 atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc
     1561 gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     1621 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     1681 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     1741 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     1801 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     1861 tcgccctaaa acaaagttgg gcgaacccgg agcctcatta attgttagcc gttaaaatta
     1921 agccctttac caaaccaata cttattatga aaaacacaat acatatcaac ttcgctattt
     1981 ttttaataat tgcaaatatt atctacagca gcgccagtgc atcaacagat atctctactg
     2041 ttgcatctcc attatttgaa ggaactgaag gttgtttttt actttacgat gcatccacaa
     2101 acgctgaaat tgctcaattc aataaagcaa agtgtgcaac gcaaatggca ccagattcaa
     2161 ctttcaagat cgcattatca cttatggcat ttgatgcgga aataatagat cagaaaacca
     2221 tattcaaatg ggataaaacc cccaaaggaa tggagatctg gaacagcaat catacaccaa
     2281 agacgtggat gcaattttct gttgtttggg tttcgcaaga aataacccaa aaaattggat
     2341 taaataaaat caagaattat ctcaaagatt ttgattatgg aaatcaagac ttctctggag
     2401 ataaagaaag aaacaacgga ttaacagaag catggctcga aagtagctta aaaatttcac
     2461 cagaagaaca aattcaattc ctgcgtaaaa ttattaatca caatctccca gttaaaaact
     2521 cagccataga aaacaccata gagaacatgt atctacaaga tctggataat agtacaaaac
     2581 tgtatgggaa aactggtgca ggattcacag caaatagaac cttacaaaac ggatggtttg
     2641 aagggtttat tataagcaaa tcaggacata aatatgtttt tgtgtccgca cttacaggaa
     2701 acttggggtc gaatttaaca tcaagcataa aagccaagaa aaatgcgatc accattctaa
     2761 acacactaaa tttataaaaa atctaatggc aaaatcgccc aacccttcaa tcaagtcggg
     2821 acggccaaaa gcaagctttt ggctcccctc gctggcgctc ggcgcccctt atttcaaacg
     2881 ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     2941 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     3001 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     3061 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     3121 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     3181 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     3241 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     3301 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     3361 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     3421 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     3481 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     3541 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     3601 cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc
     3661 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg
     3721 cggcttaact caagcgttag atgcactaag cacataattg cggtgatgct gccaacttac
     3781 tgatttagtg tatgatggtg t
//
LOCUS       DAJTPG010000413         3298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.guided.219, whole genome shotgun sequence.
ACCESSION   DAJTPG010000413 DAJTPG010000000
VERSION     DAJTPG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.guided.219"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            68..283
                     /locus_tag="OO230_004418"
     CDS             68..283
                     /locus_tag="OO230_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5693884.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            276..662
                     /locus_tag="OO230_004419"
     CDS             276..662
                     /locus_tag="OO230_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5693885.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            670..1356
                     /locus_tag="OO230_004420"
     CDS             670..1356
                     /locus_tag="OO230_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5693886.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1334..1957)
                     /gene="tetR(B)"
                     /locus_tag="OO230_004421"
     CDS             complement(1334..1957)
                     /gene="tetR(B)"
                     /locus_tag="OO230_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5693887.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2039..3244
                     /gene="tet(B)"
                     /locus_tag="OO230_004422"
     CDS             2039..3244
                     /gene="tet(B)"
                     /locus_tag="OO230_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5693888.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          902 a          610 c          734 g         1052 t
ORIGIN      
        1 tgaacataaa acactatcaa taagttggag tcattaccga ccactattaa gtcatgtagc
       61 tggttttatt tcaattgaac gttttcaaag tctagctaca gaaggaaaaa tgttatcgct
      121 atcttggtgg gaaaacgaat acgcagttct gcaatggaaa aatcatgttt tacatgcgaa
      181 agctcaacaa gaagggcgag agtcaatatt tgatttttac aaaattagta ttgctcatat
      241 tactcgcgaa tattcattta aaaaggacaa ggataatgtt tgatgttcac gttgttttag
      301 ataatcaaat aggacaatta gcattactag gaaaaacatt aggtaataaa ggtattggat
      361 tggaaggggg agggatattt acggttggtg atgaatgcca tgctcatttt cttgttgaac
      421 aaggaaagga agctaaaata gcgctagagc aagctggact gttagtactt gcgatccgga
      481 caccattaat tcgtaagtta aaacaggaaa aaccggggga acttggcgaa atagcacgag
      541 tattggcgga gaataacatt aatattttag tgcaatacag tgaccatgct aaccaactga
      601 tattaataac ggacaatgat agtatggctg catctgttac gctcccttgg gcaataaagt
      661 gaacttgcga tggctaattt aatacgaaaa gaggttacct ttgagtcctc aatagccgcg
      721 ataggggcgg ctatgtctga catttcacga gttaaaatac tcagtgcttt gatggatggg
      781 cgagcttgga cggccactga gctaagttct gtggcgaata tatcagcttc aacggcgagc
      841 agtcatttat ctaaattatt agattgccag ctaatcacag tagtagctca aggcaagcat
      901 cgttattttc ggctagcagg aaaagatatt gctgaattga tggaaagtat gatggggatc
      961 tccttaaacc atggcgtaca tgccaaagtt tccacgccag tgcatttacg aaaagcacgt
     1021 acttgctatg atcatttagc tggcgaagtt gccgttaaga tctatgattc cctttgtcaa
     1081 cagcaatgga tcactgaaaa tggttcaatg atcacattaa gtggtattca atattttcat
     1141 gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa tctgttgtgc gtgtttagat
     1201 tggagtgaac gccgtttcca tttaggtggg tacgttggag ccgcattatt ttcgctttat
     1261 gaatctaaag ggtggttaac tcgacatctt ggttaccgtg aagttaccat cacggaaaaa
     1321 ggttatgctg cttttaagac ccactttcac atttaagttg tttttctaat ccgcatatga
     1381 tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag
     1441 cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga
     1501 cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg
     1561 catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga
     1621 gagtttcata ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat
     1681 gacttagtaa agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt
     1741 ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt
     1801 cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct
     1861 tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg
     1921 cgctgttaat cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga
     1981 cactctatca ttgatagagt tattttacca ctccctatca gtgatagaga aaagtgaaat
     2041 gaatagttcg acaaagatcg cattggtaat tacgttactc gatgccatgg ggattggcct
     2101 tatcatgcca gtcttgccaa cgttattacg tgaatttatt gcttcggaag atatcgctaa
     2161 ccactttggc gtattgcttg cactttatgc gttaatgcag gttatctttg ctccttggct
     2221 tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat taataggcgc
     2281 atcgctggat tacttattgc tggctttttc aagtgcgctt tggatgctgt atttaggccg
     2341 tttgctttca gggatcacag gagctactgg ggctgtcgcg gcatcggtca ttgccgatac
     2401 cacctcagct tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt ttgggcttgg
     2461 tttaatagcg gggcctatta ttggtggttt tgcaggagag atttcaccgc atagtccctt
     2521 ttttatcgct gcgttgctaa atattgtcgc tttccttgtg gttatgtttt ggttccgtga
     2581 aaccaaaaat acacgtgata atacagatac cgaagtaggg gttgagacgc aatcgaattc
     2641 ggtatacatc actttattta aaacgatgcc cattttgttg attatttatt tttcagcgca
     2701 attgataggc caaattcccg caacggtgtg ggtgctattt accgaaaatc gttttggatg
     2761 gaatagcatg atggttggct tttcattagc gggtcttggt cttttacact cagtattcca
     2821 agcctttgtg gcaggaagaa tagccactaa atggggcgaa aaaacggcag tactgctcgg
     2881 atttattgca gatagtagtg catttgcctt tttagcgttt atatctgaag gttggttagt
     2941 tttccctgtt ttaattttat tggctggtgg tgggatcgct ttacctgcat tacagggagt
     3001 gatgtctatc caaacaaaga gtcatcagca aggtgcttta cagggattat tggtgagcct
     3061 taccaatgca accggtgtta ttggcccatt actgtttgct gttatttata atcattcact
     3121 accaatttgg gatggctgga tttggattat tggtttagcg ttttactgta ttattatcct
     3181 gctatcgatg accttcatgt taacccctca agctcagggg agtaaacagg agacaagtgc
     3241 ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata taatgaccct cttgataa
//
LOCUS       DAJTPG010000414         2989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.guided.230, whole genome shotgun sequence.
ACCESSION   DAJTPG010000414 DAJTPG010000000
VERSION     DAJTPG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.guided.230"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            <1..487
                     /gene="frdB"
                     /locus_tag="OO230_004423"
     CDS             <1..487
                     /gene="frdB"
                     /locus_tag="OO230_004423"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5693889.1"
                     /translation="DYTDGMKVEALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRT
                     ADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSR
                     DHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKP
                     R"
     gene            498..893
                     /gene="frdC"
                     /locus_tag="OO230_004424"
     CDS             498..893
                     /gene="frdC"
                     /locus_tag="OO230_004424"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5693890.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            904..1263
                     /gene="frdD"
                     /locus_tag="OO230_004425"
     CDS             904..1263
                     /gene="frdD"
                     /locus_tag="OO230_004425"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5693891.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1326..2459
                     /locus_tag="OO230_004426"
     CDS             1326..2459
                     /locus_tag="OO230_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5693892.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2548..>2989
                     /gene="blc"
                     /locus_tag="OO230_004427"
     CDS             2548..>2989
                     /gene="blc"
                     /locus_tag="OO230_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5693893.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTI"
BASE COUNT          719 a          775 c          772 g          723 t
ORIGIN      
        1 tgattacacc gacggtatga aggttgaagc gttagctaac ttcccgattg aacgcgatct
       61 ggtggtcgat atgacccact tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg
      121 caactcccgc accgcggatc agggtactaa catccagacc ccggcgcaga tggcaaagta
      181 tcaccagttc tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg
      241 cctgaaccca gagttcatcg gtccggctgc cattacgctg gcgcatcgtt ataacgaaga
      301 tagccgcgac cacggtaaga aggagcgtat ggcgcagttg aacagccaga acggtgtatg
      361 gagctgtact ttcgtgggct attgctccga agtctgcccg aaacacgtcg atccggctgc
      421 ggccattcag cagggcaaag tagaaagttc gaaagacttt cttatcgcga ccctgaaacc
      481 acgctaagga gtgcaacatg acgactaaac gtaaaccgta tgtacggcca atgacgtcca
      541 cctggtggaa aaaattgccg ttttatcgct tttacatgct gcgcgaaggc acggcggttc
      601 cggctgtgtg gttcagcatt gaactgattt tcgcgctgtt tgccctgaaa aatggcccgg
      661 aagcctgggc gggattcgtc gactttttac aaaacccggt tatcgtgatc attaacctga
      721 tcactctgac ggcagctctg ctgcacacca aaacctggtt tgaactggca ccgaaagcgg
      781 ccaatatcat tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa agtctctggg
      841 cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg taaggagcct
      901 gagatgatta atccaaatcc aaagcgttct gacgaaccgg tattctgggg cctcttcggg
      961 gccggtggta tgtggagcgc aattattgcg ccggtgatga tcctgctggt gggtattctg
     1021 ctgccactgg ggctgtttcc gggtgatgcg ctgagctacg agcgcgttct ggcgttcgcg
     1081 cagagcttca ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct gtggtgtggt
     1141 ttacaccgta tgcaccacgc gatgcacgat ctgaaaatcc acgtgcctgc gggcaaatgg
     1201 gttttctacg gtctggctgc tatcctgaca gttgtcacgc tgattggtgt cgttacaatc
     1261 taacgcatcg ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta tggaaaccag
     1321 accttatgtt caaaacgacg ctctgcgcct tattaattac cgcctcttac tccacatttg
     1381 ctgcccctca acaaatcaac gatattgtgc atcgcacaat taccccgctt atagagcaac
     1441 aaaagatccc cggtatggcg gtggcggtaa tttatcaggg taaaccttat tactttacct
     1501 ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag
     1561 gttcggtcag caaaacattt acgggcgtgc ttggtggcga cgctattgct cgaggggaaa
     1621 tcaagttaag cgatcccgca acaaaatact ggcctgaact taccgctaaa cagtggaatg
     1681 ggatcacact attacatctt gcgacctaca ccgctggcgg cctgccattg caggtgccgg
     1741 atgaagtgaa atcctcaagc gacttgctgc gcttctatca aaactggcag cctgcatggg
     1801 ctccaggaac acaacgtctg tatgccaact ccagtatcgg tttgttcggc gcactggctg
     1861 tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac tcgtgtcttc cagccactca
     1921 aactcaacca tacgtggatt aatgtacctc ccccagaaga aaagaattac gcctggggat
     1981 atcgcgaagg taaggcagtg catgtttcgc caggggcgtt agatgctgaa gcttatggtg
     2041 tgaagtcgac cattgaagat atggcccgct gggtgcaaag caatttaccc ccccttgata
     2101 tcaatgagaa aacgcttcaa caagggatac aactggcaca atctcgctac tggcaaaccg
     2161 gcgatatgta tcagggcctg ggctgggaaa tgctggactg gccggtcaat cctgacagca
     2221 tcattaacgg cagtgacaat aaaattgcac tggcagcacg cccggtaaaa gcgattaagc
     2281 ccccaactcc tgcagtacgc gcatcatggg tacataaaac gggggcgacc ggcggatttg
     2341 gtagctatgt cgcgtttatt ccagaaaaag agctgggtat cgtgatgctg gctaacaaaa
     2401 actatcccaa tccagcgaga gtcgccgccg cctgtcagat tctcaacgct ctacagtaaa
     2461 atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt catctacact
     2521 tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca
     2581 gctgcatttc tggtcgttgc ctgtagtttt cctacgccgc cgcgtggcgt gaccgtagtg
     2641 aataatttcg acgccaaacg ttatctcggt acctggtatg agattgcccg ttttgatcac
     2701 cgttttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga tgacggcggc
     2761 ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca gagtgaaggg
     2821 aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt ctttggtcct
     2881 ttctatggcg gttataacgt tattgcactc gatcgggaat accgccatgc gctggtttgc
     2941 ggcccggacc gcgactacct gtggatactc tcccgcacgc caaccattt
//
LOCUS       DAJTPG010000415         2364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 201152
            SAMEA8899911-rid15819743.guided.249, whole genome shotgun sequence.
ACCESSION   DAJTPG010000415 DAJTPG010000000
VERSION     DAJTPG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899911
            Sequence Read Archive: ERR6005243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478157.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:24:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,430
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,977
            CDSs (with protein)               :: 3,977
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 57
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 385
            CDSs (without protein)            :: 385
            Pseudo Genes (ambiguous residues) :: 0 of 385
            Pseudo Genes (frameshifted)       :: 177 of 385
            Pseudo Genes (incomplete)         :: 106 of 385
            Pseudo Genes (internal stop)      :: 147 of 385
            Pseudo Genes (multiple problems)  :: 44 of 385
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899911-rid15819743.guided.249"
                     /serovar="S. flexneri 2a"
                     /isolate="201152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Mali"
                     /collection_date="2008-12-19"
     gene            complement(<1..1333)
                     /gene="pepD"
                     /locus_tag="OO230_004428"
     CDS             complement(<1..1333)
                     /gene="pepD"
                     /locus_tag="OO230_004428"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5693894.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEM"
     gene            1595..2053
                     /gene="gpt"
                     /locus_tag="OO230_004429"
     CDS             1595..2053
                     /gene="gpt"
                     /locus_tag="OO230_004429"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5693895.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2145..>2364
                     /locus_tag="OO230_004430"
     CDS             2145..>2364
                     /locus_tag="OO230_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5693896.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARI"
BASE COUNT          559 a          618 c          589 g          598 t
ORIGIN      
        1 ccatttccgg atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga
       61 tgttcggcgt cttgttgaac aggcgctgat aggtttcacg taccagatgc atcaccggag
      121 aattagcgtc cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca
      181 gtttacccag cgaatccagc atgctcacca cgtagtcttt accgctgtcg attagtgaac
      241 ggatcaggca gtgaatttct acgttattgt cagtcatggt caccacaccg acgttcaggg
      301 aggtttcgac cacacctttt gccacgtcgg aattgcggat cacaccgttc ggggtggcgt
      361 tcagcagacg aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta
      421 cagagtccag caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct
      481 cctgataggt attcaccaga gatttcagtg cgtcgacttt atcagctgcg acagcaatgg
      541 tcgcaaaggc ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc
      601 gcaggtccag ttcttctgca tgacccgcca ggaagcgcac cagcagtttg ttggcattac
      661 ccaggccaac gtggatttca ccgccggagt gaccgccttt cagacctttt aaggttaact
      721 tgaaggtttc aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt
      781 cgataccccc cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca
      841 gaatatcagc ctgcaaccag ttgctctgta agccgaacgc accgtccata ccggcttctt
      901 cggtcatggt cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaaccg
      961 ccagtgcaga ggccatacca atgccgttat ccgcacccag cgtggtgccg cgcgctttaa
     1021 cccattcgcc atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt
     1081 tatttttctg cggcaccata tcgaggtggg cctgtaagac gaccggttta cgattttcca
     1141 tacctgcggt agcaggttga cgaatcagga tattacctac ctgatcgcgt tcgacatgga
     1201 aacctttctc ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg
     1261 ggtgaggaat agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag
     1321 acagttcaga cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca
     1381 gggttagcag aaaatgttgt caacacaaga caggcttgcg agatatgtat tgagaatacc
     1441 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     1501 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     1561 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
     1621 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
     1681 gaaaggcatt attgccgtaa gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga
     1741 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     1801 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     1861 cctggtggat accggtggta ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt
     1921 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     1981 cccgcaagat acctggatcg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     2041 ctccggtcgc taatcttttc aacgcctggc attgccgggc gttgttcttt ttaacttcag
     2101 gcaggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     2161 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     2221 gctttaatca cggcgcacaa ccgcctgtgc agtcggctct tgatggtaaa accatccctc
     2281 actggtatcg catgattaac cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa
     2341 tcctcgacgt ccaggcacgt attg
//
